LOCUS       DAOQTR010000001        19577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_3240_length_19577_cov_3.492060, whole genome shotgun sequence.
ACCESSION   DAOQTR010000001 DAOQTR010000000
VERSION     DAOQTR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 19577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19577
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_19577_cov_3.492060"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1870)
                     /gene="recJ"
                     /locus_tag="PKN85_00005"
     CDS             complement(179..1870)
                     /gene="recJ"
                     /locus_tag="PKN85_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNT42812.1"
                     /translation="MDKGGPEGVAHGVPGEEEAVRIVREIAEGAGVPELIARILVARG
                     VRTPDEAHVFLRPRLADLSDPFLLPDMDKGVARLVEAVDRGERVCIWGDYDADGISCL
                     ALMFNFLNHLGLVPVVHIPTREEGYGLHIDKVEDLAKQGVDLIVSLDCGASNVEEIAH
                     ARSLGVDVIVVDHHEMGDEVPPAVAVINPKRRGSRFPTRDLAACGVTFFFLLGLRRVM
                     HGKGLIPAHINLKKELDLVTVGTVGDMVPLLGDNRILVRHGMEVMNAKPRAWLKSMLD
                     SRVISKGLINEFALGFIIVPRINAAGRVASPHPALDFLVAKETGSASQLLAHLQKLNN
                     VRQQREKKIHEEIVASIGRDGLSGRKTIVVFDEKWHAGVLGIVAQKLTEQFERPAIVI
                     TRVNGIWKGSGRGGDGMDLFETIGSVSHLLLKYDDHKYACGISLKKKNLPLFAEAFEK
                     TVEGNITARRRVVHVDTAAGFEELTAEVMERLEDLGPFGIGNPRPNLALEAAGVFAAP
                     NGRTRITDGDNRTWYGFYAPKGSLQREGGARIIASPVVKMEMGERFIHLNIKEVE"
     gene            complement(1863..2765)
                     /locus_tag="PKN85_00010"
     CDS             complement(1863..2765)
                     /locus_tag="PKN85_00010"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNT42813.1"
                     /translation="MGSLAVELFGKTLKNPVMNASGTLGYGVEIEPLWAVETMGAYVT
                     KGLSAAPHHGNPTPRVWEERCGMLNSIGLQNVGVERFFSHYFPFFVERRCPVVVNFFG
                     FSEEEYLSCAKTIEPHDLIVALEMNLSCPNIKKGGISFGKEASAVHAIVRSVREVTKI
                     PLIAKLTPEVKNLVEIARAAYEGGADGLTLINTIPAAVVDVKDRRVSLKGGLSGPLLK
                     PIALRAVHECSQAVPIPIIGVGGITNAEDALAFLMAGARAVQVGCATFVDPFAMPKII
                     AGLDSWLDASGLAGISAIVGAAHG"
     gene            complement(2747..3505)
                     /locus_tag="PKN85_00015"
     CDS             complement(2747..3505)
                     /locus_tag="PKN85_00015"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNT42814.1"
                     /translation="MDDIPGKITWNDCVTGSYRRLSVRLERSLGRVSPGQFVMVKIPD
                     RDVFLRRPFSIYDSRGRTLTIVYRVVGKGTDALSAAARGTPLMVLGPLGRGFTPGKAT
                     TPIVVAGGIGYAGVRMLLKRLDHRATLFFGATGKDELRLAEDLGATRVLASTMDGSFG
                     FAGDVVSLLGHHLDDYRDKKPEVFACGPSGMVRSLKALLEPGRIPCQVSVEERMACGM
                     GLCFGCVVATADETNPYKRVCKEGPVFSLWDLSL"
     gene            complement(3670..4152)
                     /gene="rimI"
                     /locus_tag="PKN85_00020"
     CDS             complement(3670..4152)
                     /gene="rimI"
                     /locus_tag="PKN85_00020"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNT42815.1"
                     /translation="MAAGPDMTEGGEVVMRQMTVGDIDGVLEIEKVSFAAPWTRGMFE
                     ETLSSPVGISFVAEENGRIIGYLIFYRAAREIHVMNIAVDKNRRGLGLASWMMSRVMD
                     LGRRNSMETCFLEVRESNMPARSLYEKLGFRQIGRRKGYYRETGEDAIVMELTLTDQT
                     "
     gene            complement(4136..4720)
                     /locus_tag="PKN85_00025"
     CDS             complement(4136..4720)
                     /locus_tag="PKN85_00025"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNT42816.1"
                     /translation="MKIGCAILAGGRSVRMGRDKATAEFRGAPLIRQVYDTVRQVFDD
                     IVIVSSVHEAIEGVEAPVIRDILPVQSPMVGIATALIHSTRPRVFVVACDMPLLCVEA
                     VRCLVDEAEGEDITIPMAEGYYQPLHAIYGRSCLAPLLRLIGLNRLKVSDVLPYAVVK
                     AVRDRPCFYTPGGTLVFSNINTPEELAGIDGSGA"
     gene            5043..>5408
                     /locus_tag="PKN85_00030"
     CDS             5043..>5408
                     /locus_tag="PKN85_00030"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT42817.1"
                     /translation="MGAILWITEKLSKWMSAIAGAALTAIMLLTVCDVFLRYFFDRPI
                     IGTFEMVGFGGAIAIGFAIPITSWMRGHIFVDFFVQKFNKVGQGIVNVITRLASIGLF
                     YLIGYNLLKYGHDLFVSGEV"
     assembly_gap    5409..5418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5521..6828
                     /locus_tag="PKN85_00035"
     CDS             5521..6828
                     /locus_tag="PKN85_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNT42818.1"
                     /translation="MNDPITVGIVGLIIVLFLFLTGIELGFAMVIMGFLGFGYLISFS
                     AAFNLLAKDMYDVLNSYSFTVIPLFVLMGQVAFNSGIAKRLFDAAHKFIGHIPGGLAM
                     ATVGGATVFKAICGSSPATSATFASVAVPEMDRFGYDKRLSTGIVATVGTLGILLPPS
                     VTLILYGIITDQSIGRLFLAGIVPGLVIAALFIGVIYGWCKMNPAIGPASPKATWGER
                     FKTIPQVIMVLIIFFLVIGGILGGVFTPTEAGSVGTFLVLVMTFAMRDLNIRGYIKSV
                     AESVRTATMVLMLIAGSTVLGHFIAVTTIPMVAADWIVSLNMPSWVIIVFIALIYLIG
                     GSFIDDLAFMILATPIFYPAILKLGYDPMWFGILIGITVMLGVVLPPMAICVFVVKQI
                     TKTPFSVVYAGVYPFLISIIVGGILVFIFPALSTWLPHWLMPG"
     gene            complement(7364..7816)
                     /locus_tag="PKN85_00040"
     CDS             complement(7364..7816)
                     /locus_tag="PKN85_00040"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNT42819.1"
                     /translation="MIIEGGFTIKAPIDKLFDFLLKPETILTCLPGAESANMIDDTSY
                     ECIVKQKVGPIKARLKFVNKMTVVNKPTHIEIEGEGEDMTKLGHFKQKTVVDLTEKGN
                     GEVEVTYKTDVSIVGKLAMFGDRIMRAKAKDTEKEFTKNLQEKLKSIA"
     gene            complement(7871..8086)
                     /locus_tag="PKN85_00045"
     CDS             complement(7871..8086)
                     /locus_tag="PKN85_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42820.1"
                     /translation="MSFKCPACKKEWPNSKQVARHMFGTGDKAHRAWIESQGYSYIEL
                     LLAQTTEPGNKSYEILADLIEKAQDKL"
     gene            complement(8117..10396)
                     /locus_tag="PKN85_00050"
     CDS             complement(8117..10396)
                     /locus_tag="PKN85_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT42821.1"
                     /translation="MSHKKFSVINTPVHNIDGIAKVTGRATYTFDVKLPGMLYGKILR
                     SPHPHAKIINIDTTRAMELPGVHAVVTGKDTLGIKQGIWRRYEELCDEQILPLEKVRY
                     IGEPVAAVAAVTEEIAEAALDLIDVEYEVLPAVYEPLEAIKKDAPTIHEGVERNINVT
                     RHIEWGDVDEGFEQADYIREDWFKCSGQHHMCMETRAAVSNFTPEGKLTVWTSTQSAY
                     YHQALLAGVFGLREGDVRILAPYVGGGFGGKFEIDSAQFCSGILSMKLHKPVKIVLTR
                     EEDFIASKRRTPMFYYVRTGMKKDGTFVARESRMFSNGGAYTGMGATALYLSGFFHSF
                     PYKWQSYRYDGYRVYTNTMPSTSMRGFGAPQAMFCSEQQIDWMCADLGLDPFEVRRKN
                     GHTEGYEVPGQAFIASCGLEQCINEIEKWVKSKGKLPANRGIGISSCGFMSGGIFNWF
                     DSPYSFSSAVITINVDGTVELHVGCQDIGQGSNTTMAIIAAETLGVKVEDIRVHSGDT
                     AHCPADLGAWGSRQTLMAGNAVKMACEDAKKQLLEFAYAEMGINIVYDLDIKDRWVHI
                     VARPERGMKYEDLVKKALRAKDGQRIMGRGYYTPHRKGMISPAYSYMMQAVDAEIDPE
                     TGKIKIHEVMTAHDCGQPINPLGLIGQLEGAASMALGYAYLEDMPFEDGKMMNPNLVD
                     YKVIRAPEMPKTDIIEIDTYEPEGPYGAKEAGEGLTNPTAAALGNAVFNALGIQMKEL
                     PITPERVRKALAEKKEAQK"
     gene            complement(10398..10883)
                     /locus_tag="PKN85_00055"
     CDS             complement(10398..10883)
                     /locus_tag="PKN85_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT42822.1"
                     /translation="MKKQVLTFRVNGDEYEILIHPKTLLADAIREHVGLTGTKRGCDT
                     VSCGACTVMVNGMSVKSCSVLAIQCEGMDITTVEGLEENGKLSPIQRAFLDNGSYQCG
                     FCTPGMLMSSTAFLNEMGDKKPSEEEIRESIEGNICRCTGYNSIVRAIDAVAQGKYKE
                     E"
     gene            complement(10895..11767)
                     /locus_tag="PKN85_00060"
     CDS             complement(10895..11767)
                     /locus_tag="PKN85_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNT42823.1"
                     /translation="MINDFQYLKPASLKEALAMYAEHDDCKVICGGQSLLIVLRQGML
                     APEYLVDIKHVNELKYIKFDEKDGLRIGATTTHREVEKSDIIAKKYPALVAMEQKLAS
                     IQTRNWGTIGGNLAHADPAGDLAPVLIALNAEIKFGNVDGEKTVPLDEFFVDYFETQM
                     GHGDLVLEAHVPAPEPKSGCAYQKFNLLDSEMGIVAAAASVVLNADGTCKNARIVLGN
                     AGPTPLRVTKAEDLLKGKKLDDKLFTEAGQIASEEAQPVADIHASEEHRRHVLGVLTK
                     RMLKKAFEQAQKAA"
     gene            complement(11837..12067)
                     /locus_tag="PKN85_00065"
     CDS             complement(11837..12067)
                     /locus_tag="PKN85_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42824.1"
                     /translation="MECAIYRGRHYYLCYSPAYRGTSFDPERIKELKKEESADESDKT
                     FGMPGNIEVGSQCIRNVEEYGIEREFAALKKN"
     gene            12379..13401
                     /locus_tag="PKN85_00070"
     CDS             12379..13401
                     /locus_tag="PKN85_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT42825.1"
                     /translation="MKRFFGVKCLLFVFFAATCLAFFPVMASAQKVITLNYSNFFPAP
                     HKNSVVAQQWCKEVEKRSGGKVKITYFPGGTLTPAAQTYDNVVKGIADIGESCFAYTR
                     GKFPMMEVIDLPLGYKSGVSATNLINAYYAKFNPKELADVKVLWLHAHGPGILLSKKP
                     VKTMDDMKGMKIRATGLAAKVVQALGGAPVGTTMPETYDALRTGVADGAMAPVEAAQG
                     WKWGEVVKYITQNFGSAYTTGMFVVMNKAKWNAMPPDVQKVFEEVSKEWVVKQGKVWD
                     EIDKEGYDFAKKRGNQVITLSKEEDAKWAKAVKPLLDDHVKTLKGKGLPGAEAVKFAV
                     DYLKKN"
     gene            complement(13571..14179)
                     /gene="hisH"
                     /locus_tag="PKN85_00075"
     CDS             complement(13571..14179)
                     /gene="hisH"
                     /locus_tag="PKN85_00075"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNT42826.1"
                     /translation="MIVVVDYGMGNLKSVLNAFAKLGAGVTVSGDPASIEGASAIVVP
                     GVGAFGRCMENLEGRGLAPAIREHIRRDKRYLGICLGMQVLFESSEEAPGVKGLGVIK
                     GTVPRFTGSMKVPHMGWNSVDIRKESPILKGINPGDFFYFVHSYYCAPDDGSVIATTT
                     EYDGPFTSAVETGSLFACQFHPEKSQRVGLALLRNFLALAGC"
     gene            complement(14180..14764)
                     /gene="hisB"
                     /locus_tag="PKN85_00080"
     CDS             complement(14180..14764)
                     /gene="hisB"
                     /locus_tag="PKN85_00080"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNT42827.1"
                     /translation="MARKAEVERTTKETSISVSWDLDGSGEYSISTGIPFFDHMLDLF
                     AKHGLFDLVVTAKGDTDVDNHHTVEDVGIAMGKALRDALGGMEGIRRYGSALVPMDET
                     LCMTAVDISGRSSFVFKGRLQGRTGGFDVDVVKEFLQAFVNEARLTLHVNLLYGSNLH
                     HKVEAVFKSFARTLRAAVEKDDRIKGVLSTKGVL"
     gene            complement(14774..15643)
                     /gene="hisG"
                     /locus_tag="PKN85_00085"
     CDS             complement(14774..15643)
                     /gene="hisG"
                     /locus_tag="PKN85_00085"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNT42828.1"
                     /translation="MKLKIGLPKGSLQESTFRLFKNAGYTIKLRERSYVPVIDDDEME
                     GLVIRAQEMARYVEDGILDMGITGADWVLEQDARVVELVRLRYGKVGFRGVKWVVAVP
                     NNSPIKKVKDLQGKKIATELVAFTKRYLKKQGIEASVEFSWGATEVKPPLLADAIVEV
                     TETGASLVANNLRIVETILESETVLIANRTAWKDPWKKRKMENLIILLKGALLAEEKV
                     GLKMNIPRNKLGRVTEILPSLHTPTVSNLSDDKWVAIEVIIDEKVVRDIIPDLKRAGA
                     QGIVEYPLKKVIM"
     gene            complement(15640..16920)
                     /gene="hisD"
                     /locus_tag="PKN85_00090"
     CDS             complement(15640..16920)
                     /gene="hisD"
                     /locus_tag="PKN85_00090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNT42829.1"
                     /translation="MKVWELDREFDGLISFVKTGREGKKRDVREAVLKIRQAVLSRGD
                     AALVDFSIAWDGWKAAHPLKLTSREIGEAASRVPARDISVLKGMIRNVTSYHKGQKMP
                     KRVFRKKGLVVEEGPVPVESAVVYVPGGTASYPSSLVMGVVPAQIAGVKRVCATTPGR
                     GGAINDHVAAAAKLLGIDEVYRVGGAQAIYAFAYGTGSVPKVDMIVGPGNAYVEEAKR
                     DVYGSVGIDMLAGPTELVVLCTEAHSPKMVALDMFSQAEHDELASVGLFSSSREHLSD
                     VMKSIEGLIDGAPRRETIRKALDDNGYMVHYTDLGRAVEAINSIAPEHMELLGDTTCL
                     PSILYPGVIYVGLNTPVAMGDYYIGTNHVLPTAGAGRFTGGLSVDRFTKRKVVVTIDR
                     TFIEKYGDRAERLARIEGLPAHGDAIKARKESPK"
     gene            complement(16901..18175)
                     /gene="murA"
                     /locus_tag="PKN85_00095"
     CDS             complement(16901..18175)
                     /gene="murA"
                     /locus_tag="PKN85_00095"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT42830.1"
                     /translation="MDKFIIEGGERLKGKVRISGSKNAVLPVLAATLLQRGTYAISNV
                     PRLMDVTTMMQLLDLLGARCVWQDEHTVEVDTAEVGNHVAPYELVKKMRASVLVLGAL
                     IGGLRKATVSYPGGCAIGERPIDLHLKGLSALGCDVAIREGYVDVTAKKLKGARIVFD
                     SVTVGGTENLLMAAVFAKGETLIENAAREPEVVDLARMLKKMGARIEGEGTAVIRILG
                     VGELTPCAWDVIEDRIEAGTFLAACGMTRGSIVLENCVPEHIKAVIDKLTETGMDIHC
                     DGRTIKASMSKRRPLAVDIRTVPYPGFPTDMQAQLMAAMTTSKGVSAITETIFENRMM
                     HAAELRRMGADIRVVGNTAIVHGVESMSGAKVMATDLRASASLIIAGLAAYGKTEVSR
                     IYHIDRGYERIEEKLKNLGARIERVKDESMGA"
     gene            complement(18168..18986)
                     /gene="prmC"
                     /locus_tag="PKN85_00100"
     CDS             complement(18168..18986)
                     /gene="prmC"
                     /locus_tag="PKN85_00100"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNT42831.1"
                     /translation="MRISQIIAGERGLSRTDVIATVCLALGLTKEQLFCRMDREIGGE
                     DLGAVRKALDERAAGRPLAYMTGTREFFSETFSVNEAVLIPRPETEVLVEEALALIGG
                     RRDLRILDMGTGSGAIGIVIAKHAGSSVVCVDISEAALAVARRNARSIGVEERTRFVC
                     GDLFAAFSDGTRFDMVLSNPPYVADDEWDELMADVRDFEPARALRGGEGGLEVYRRIA
                     AELPRRLAPGGSVLLEIGSEAQAGTVGAMLEVAGLRVKVRKDYSGRERVLAGHG"
     gene            complement(18993..>19577)
                     /locus_tag="PKN85_00105"
     CDS             complement(18993..>19577)
                     /locus_tag="PKN85_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNT42832.1"
                     /translation="VIVVIEGKDAFNTLRYESGVHRVQRVPETEAQGRIHTSTITVAV
                     LPEPEELEVTINPDEVRVDLFRSSGPGGQHVNTTDSAVRLTHIPTGMVVTCQDEKSQH
                     KNKAKAMRVLRARLKEKMEGEKEQEISDERRKQVGTGERSERIRTYNFPQGRVTDHRI
                     GLTLYKLQDILNGNIEEITGPVTAHFRSEALKKG"
BASE COUNT         3594 a         6002 c         5498 g         4473 t
ORIGIN      
        1 agaaaagccg gacgccgttt ttcctgtctt tgcctaacac gtaaaacgta aaacctaaaa
       61 cggcttttct accgttttaa gttttaagtg ttaagtttta agttaaaagg agaaaagccg
      121 gacgccgttt ttcctgtctt tgcctaacac gtaaaacgta aaacccaaaa cggcttttct
      181 attctacttc ttttatattg aggtgtatga agcgttctcc catttccatt ttgacgacgg
      241 gggaggcgat tatccttgcg ccgccttccc tctggaggga accttttggt gcgtagaagc
      301 cgtaccaggt cctgttgtcc ccatcggtga tcctggtgcg tccgttcggc gcggcgaaga
      361 cgcccgcagc ctcaagggca aggttcgggc ggggattgcc gatgccgaag gggccgaggt
      421 cttccagtct ttccatcacc tccgccgtaa gctcctcgaa tccggcggcg gtgtccacgt
      481 gcaccacacg ccttcgcgct gtgatattcc cctccaccgt cttttcgaac gcctcggcaa
      541 agagcggcag gttcttcttt tttagtgaga tgccgcatgc atacttgtga tcgtcgtatt
      601 tcagaaggag atgcgagacg gagcctatgg tctcaaaaag gtccatgccg tcgcctcccc
      661 ttccggaacc cttccatatc ccgttgaccc tggtaatgac gatggctggc ctttcgaact
      721 gctcggtcag cttttgggct acaatgccca ggacgcccgc gtgccacttt tcatcgaaga
      781 cgacgatcgt cttcctgccc gaaagaccgt cgcggcctat ggaagcgacg atctcctcgt
      841 gtatcttctt ctcccgctgc tgcctcacgt tgttcagctt ctgcaggtgc gcgagcagtt
      901 gagacgccga cccggtctcc tttgccacca ggaaatcgag ggccggatgg gggctcgcaa
      961 ccctgccggc ggcattgatc cggggaacga tgatgaaacc aagggcaaat tcgttgatga
     1021 gccccttcga tatgaccctg ctgtcgagca tcgacttgag ccatgcccgc ggcttcgcat
     1081 tcatgacctc catgccgtgg cgcacgagta tcctgttgtc ccccaaaagc ggcaccatgt
     1141 cccccacggt ccccacggtc acgaggtcga gctctttctt gaggttgatg tgcgccggaa
     1201 tgagaccttt tccgtgcatc acgcggcgca gcccgaggag aaagaagaag gtgacgccgc
     1261 acgcggcgag gtccctcgtg ggaaaccggg aaccgcggcg tttcgggttt atgactgcca
     1321 ccgccggggg aacctcgtca cccatctcgt gatggtcaac gacgatgacg tccacgccga
     1381 ggctcctggc atgggctatc tcctcgacgt tggaggcacc gcagtcgagg gaaacgataa
     1441 ggtccacgcc ctgtttagcg aggtcctcca ccttgtcgat gtgaagcccg tatccctcct
     1501 cccgggtggg tatgtggacg acgggcacga gtcccagatg gttcaggaaa ttgaacatca
     1561 aggcgaggca ggaaatgccg tccgcatcgt agtcgcccca tatgcagacg cgctcccccc
     1621 tgtcgaccgc ctcgacaagc cgcgcgacgc ccttgtccat atcgggcagg aggaaggggt
     1681 cggacaggtc ggcgagccgg ggcctcagga agacatgggc ttcgtcgggg gtcctcaccc
     1741 cccgggcaac gaggatgcgc gcgatgagct cgggcacgcc cgcgccttcc gctatctcgc
     1801 ggacgatccg cacggcctct tcctcgcccg ggaccccatg ggcgacacct tccggaccgc
     1861 ccttatccat gggcggcccc cacgatggcg gatatcccgg cgagtcccga tgcatcgagc
     1921 caggaatcaa gacccgcgat gatcttcggc atggcaaagg gatccacgaa cgtggcacat
     1981 cccacctgca ccgcgcgggc gcccgccatg aggaacgcca gggcgtcctc ggcgttcgtt
     2041 atcccgccga ccccgatgat gggtatgggg accgcctgtg aacactcgtg caccgcgcgc
     2101 aacgcgatag gtttcaggag gggcccggaa agaccgccct tcagagagac cctgcggtcc
     2161 ttcacgtcga caacggcggc gggtatcgtg tttatgagcg tcaatccgtc cgctcctccc
     2221 tcgtaggcgg cgcgggcgat ctccacgagg ttcttcacct ccggcgtcaa cttcgcgatg
     2281 aggggtatct tcgtgacctc ccgcacggaa cgcacgatgg cgtggaccgc cgacgcctcc
     2341 ttcccgaagc tgatgccgcc ttttttaata ttgggacagg aaaggttcat ctccagcgcc
     2401 acgatgaggt cgtggggttc tatggttttc gcgcaggaaa ggtattcctc ctcgctgaaa
     2461 ccgaagaagt tgacgacgac ggggcaccgc ctctctacga agaaaggaaa ataatgggag
     2521 aagaatcgct cgacgcccac gttctggagg ccgatggagt tcagcatccc gcagcgttcc
     2581 tcccagacgc ggggcgtggg gttgccgtga tggggcgcgg cggagaggcc cttcgtcacg
     2641 taggctccca tggtctccac cgcccagagc ggttctatct cgacgccgta gccgagcgtg
     2701 cccgacgcgt tcatgacggg gttcttgagc gttttgccga aaagctctac agcgagagat
     2761 cccataggct gaataccggt ccttccttgc acacccgctt gtaggggttc gtctcgtccg
     2821 cggtggcgac gacgcagccg aaacacaggc ccatgccgca tgccattctc tcttcaacgg
     2881 aaacctgaca gggaatcctc ccgggctcca ggagggcctt gaggctcctt accatacccg
     2941 aagggccgca ggcgaagacc tccggcttct tatcccggta gtcatcgagg tggtgcccga
     3001 gaaggctcac cacatcgccg gcgaacccga aggacccgtc catggtcgag gcgaggaccc
     3061 tcgttgctcc aaggtcttcc gccagacgaa gctcatcctt ccccgtggcc ccgaagaaga
     3121 gggtggccct gtgatcgagc cgtttgagga gcattctgac accggcataa ccgatgcctc
     3181 cggcgaccac gatgggggtg gtagcttttc ccggcgtgaa cccgcggccg agagggccga
     3241 ggaccatgag cggtgttccc ctggcggcgg cgctcaaggc atcggtgcct ttgcccacca
     3301 cccggtacac gatcgtcagc gttcgtcccc gggagtcata gatgctgaag ggcctgcgca
     3361 ggaacacgtc gcggtccggg atcttcacca tcacaaactg gcccggtgat accctgccga
     3421 gagacctctc gagtctcacg ctgagacggc ggtagctgcc cgtgacgcag tcgttccatg
     3481 tgatctttcc cggaatgtca tccatggctt ccctttgttc cccggtacgc cataccatta
     3541 atatatatgg gggcggtgtc ttgggtaagc gattgtaacc cgtatggcct gccgggcgca
     3601 acttaaataa tgcgggggtc gggatgaaga aacggcccgt gcgggcctga cgcttctcct
     3661 cctttcgtgt caggtctgat cggtcagtgt cagttccatc acgatggcgt cctcgccggt
     3721 ctcccggtaa tatcccttgc gcctgccgat ctgcctgaag ccaagcttct cgtagaggct
     3781 tcgggcgggc atgttgcttt ccctcacctc gagaaaacag gtctccatgg aattcctccg
     3841 cccgaggtcc atgacgcggg acatcatcca cgacgcaagg ccgagcccgc gccggttctt
     3901 atcgacggct atgttcatga cgtgtatctc ccgtgcggca cgatagaaga tgaggtaccc
     3961 gatgatccgc ccattctctt cggcgacgaa gctaatgccg acaggcgacg agagggtctc
     4021 ttcgaacatt ccccgtgtcc agggcgcggc gaaggaaacc ttttctatct caaggacgcc
     4081 gtcgatgtcg ccgacggtca tctggcgcat caccacctcg ccgccctcgg tcatgtcagg
     4141 ccccgctgcc atcgatcccg gcaagctcct ccggcgtatt gatgttcgaa aagacaaggg
     4201 ttccccccgg ggtgtagaag catggacggt ccctgacggc ttttacgacc gcgtagggca
     4261 ggacgtcgga gaccttgagt cggttaaggc ctataagcct caaaaggggt gcgaggcagg
     4321 aacgcccgta tatggcgtga agtggctggt agtagccttc ggccatggga atggtgatat
     4381 cctctccttc cgcttcatcg acaaggcatc ggaccgcctc gacgcaaagc agcggcatgt
     4441 cgcaggcgac cacaaagacg cggggcctcg tggaatggat aagcgccgtc gcgatgccca
     4501 ccatggggct ttgcacgggt agaatgtccc gtatgaccgg agcctcgacg ccttcgatgg
     4561 cctcgtggac cgaggagacg atgacgatgt cgtcgaagac ctgcctcacc gtgtcgtaga
     4621 cctgccggat gaggggggcg ccgcggaact cggccgtcgc cttgtcgcgc cccatccgca
     4681 cgctccttcc ccccgccaaa atggcgcagc ctatcttcat gcacaaggta ttaccatctt
     4741 tcgctccgaa tatcaaaggc ctgttcgact gtgcaccgca caggagagag tgaccgacaa
     4801 actgtttttt gccccttcat gcgttgcctt tatcccgtca ataaaggctt tgtccagtgt
     4861 ttacgcgacg ttatcatttc cagtcccggc gaactggggt acaaaacctg acaaaatcca
     4921 acgattctga taagttaggt tgtaactttt ttcacttgac attgctcgcc gggatgcctt
     4981 aagatgccat aatatatcca ggggtacaag agacaataca aatagtttag ttaggaggag
     5041 caatgggagc catactttgg atcactgaaa agctcagtaa gtggatgagc gccattgccg
     5101 gggcagcctt gaccgctatc atgctgctca cggtatgtga cgttttcctt cgctatttct
     5161 tcgacaggcc cattatcggt acctttgaga tggtcggctt cggcggggcg atagccatcg
     5221 gtttcgccat ccccatcacc tcgtggatga ggggccacat ctttgtcgat ttcttcgtcc
     5281 agaagttcaa caaggtgggg cagggcatag tcaacgtcat cacccgtctc gcaagcatcg
     5341 gcctttttta cctgatcggt tataacctcc tcaagtacgg tcacgacctc ttcgtgtccg
     5401 gagaggtcnn nnnnnnnngg cagatcccct tttatcctat cgcgtacgga atggccatat
     5461 gctgtttcat acagtgtctg gtcctgatct gcgacctttt caagatcttt ggaggccaat
     5521 atgaatgatc ccataaccgt tggaatcgtt ggactgatca tagtactctt tctcttcctc
     5581 accggcatcg agctcggctt cgccatggtg ataatgggtt ttctcggctt cggctacctc
     5641 atttcattca gcgccgcgtt caacctgctg gcaaaggaca tgtacgacgt cctcaactct
     5701 tacagcttca ctgtcatacc cctgtttgtt ctcatgggac aggtggcctt caactcgggt
     5761 atagcgaaac gcctctttga cgcggcccac aagttcatcg gccacatccc cggcgggctc
     5821 gccatggcga ccgtcggcgg cgcaaccgtt ttcaaggcca tctgcggttc ctcgccggcc
     5881 acgtccgcga cgtttgccag cgtcgccgtc cccgagatgg accggtttgg ctacgacaag
     5941 agactctcca cgggtatcgt ggcaaccgtc ggcacactcg gcatactcct tccgcctagt
     6001 gttaccctca tcctctacgg catcatcact gaccaatcca tcggcaggct cttcctcgcc
     6061 ggcatcgttc ccggtctcgt gattgccgcc ctcttcatcg gtgtcattta cggatggtgc
     6121 aagatgaacc ccgccatagg tcccgcgagt cccaaggcaa cctgggggga gcgtttcaag
     6181 accataccgc aggtcatcat ggtccttatc atctttttcc tcgttatcgg cggcatcctc
     6241 ggcggcgtct tcacgcccac agaggccggt agtgtcggca ccttcctcgt tctcgtcatg
     6301 accttcgcca tgcgcgacct caacataaga ggttatatca agtccgttgc cgagtccgtc
     6361 agaaccgcca ccatggtcct catgctcata gccggctcca ctgtcctcgg tcatttcatc
     6421 gccgtcacga cgatacccat ggtcgccgcg gactggatcg tcagcctcaa catgccctcc
     6481 tgggtcatca tcgtcttcat cgccctcatc tacctcatcg gcggttcatt catcgatgac
     6541 ctggccttca tgatcctggc gacgcccatc ttctaccccg cgatactcaa actcggatac
     6601 gaccccatgt ggttcggcat cctcatcggc ataacggtca tgctcggcgt cgttctccct
     6661 cccatggcca tctgtgtctt cgtcgtcaag cagatcacca agacaccctt cagcgtcgtc
     6721 tatgccggcg tctacccctt cctcatcagc atcatcgtcg gcggcatcct ggtcttcatc
     6781 ttccccgcgc tttcgacctg gctcccccat tggctgatgc cgggctgaca aaaaaggatt
     6841 gaaggcaaaa accgcctccc tcaggaggcg gttttttttc tccaatatcc tcctgatata
     6901 gttctctttt acatcattcc gcctatcgtc ccctcctcct ctctgcactc cccattcccc
     6961 gtcatcccgc cattcttcct tctccgtcat tccggcgcag gccggaatct aggaaggcct
     7021 ggcaatgaat gaacgctgaa aggttcgatg gcacctctcg tatcgtgctc ttcggtcagt
     7081 atccttatgt ttgctttctg ccgcaaccga gtttttcctg gttcccggcc tgcgccggga
     7141 tgacggactg agagtgtgga gggatgatga cgaagggcgg ggcgggaaac ggggtttgtc
     7201 aacagcctgt cgagcggtgc aagtgaaaga cggctagacc cgacggcctc tgaacacgat
     7261 tggcacaccg gtggaaggtg cccgttgcct tgttttgtct tttggtaacg aaaaaggggt
     7321 tcccgcacag ggcgggaacc ccttttcttc ttcgcattat ttactacgct atggatttca
     7381 gcttttcctg aaggttcttc gtgaattcct tttccgtgtc cttcgccttc gccctcatta
     7441 tcctgtcgcc gaacatggcg agcttgccga cgatgctcac gtctgtcttg tatgtgacct
     7501 cgacctcgcc gttgcccttt tctgtcaggt caacgaccgt cttctgtttg aagtggccga
     7561 gtttggtcat atcttcgccc tcaccttcga tctcgatgtg cgtgggcttg ttgacaacgg
     7621 tcatcttgtt cacgaatttc agcctggcct tgatgggccc gactttctgc ttcacgatgc
     7681 actcgtatga cgtgtcgtcg atcatattcg ccgactctgc gcccggaagg caggtgagga
     7741 tcgtttccgg tttcagcagg aaatcgaaga gcttatcgat cggtgctttt atggtaaaac
     7801 cgccctctat gatcatggga cttcctcctt aatgttataa ttatcaagcc tttcgcgggc
     7861 tcaattattc ttacagcttg tcctgcgcct tctcgataag gtccgcaagg atctcgtagc
     7921 tcttgttgcc cggctcggtt gtctgagcga ggaggagctc gatatatgag taaccctgag
     7981 attcgatcca tgcccggtgc gccttgtcgc cggtaccgaa catgtggcgc gccacctgct
     8041 tgctgttggg ccattctttt ttacatgcgg gacatttaaa gctcacggta tacctcctta
     8101 tttgtctttg tcagggctat ttctgggctt ccttcttctc ggccagggcc ttgcggaccc
     8161 tttccggggt gatgggaagc tctttcatct ggatgcccag ggcgttgaaa acagcgttgc
     8221 caagcgcggc cgccgtcggg ttcgtcaggc cttcgccggc ctctttcgcg ccgtagggtc
     8281 cttccggctc gtaggtatcg atctcgatga tatccgtctt cggcatctcg ggggccctga
     8341 tgaccttata gtcgaccagg ttggggttca tcatcttgcc gtcctcaaaa ggcatgtcct
     8401 cgagataggc gtagccgagg gccatggaag ccgcgccctc gagctgaccg atgagtccca
     8461 gggggttgat gggctggccg cagtcgtgtg ccgtcatgac ctcgtggatc ttgatctttc
     8521 ccgtttcggg gtcgatctcg gcgtcgacag cctgcatcat gtagctgtat gcgggggata
     8581 tcatgccttt tctgtgaggc gtgtagtagc cgcggcccat gatgcgctgg ccgtctttcg
     8641 cgcggagcgc cttcttgacg aggtcttcgt atttcatgcc tctttccgga cgggcgacga
     8701 tatgcaccca cctgtccttg atgtcgaggt catagacgat gttgatgccc atttccgcgt
     8761 aggcaaattc caggagctgc ttcttcgcgt cctcacaggc catcttcacg gcgttgcctg
     8821 ccatgagggt ctggcgtgag ccccatgcgc cgaggtcggc ggggcagtgc gcggtgtcgc
     8881 cggagtggac cctgatgtct tccactttca cgcccagggt ctctgccgcg atgatcgcca
     8941 tcgtggtgtt cgagccctgg ccgatatcct ggcagcccac atggagctcg acggtaccgt
     9001 caacgttgat ggtgatgaca gccgaggaga atgaataggg tgagtcgaac cagttgaaga
     9061 tgccgcccga catgaaaccg caggaggata tgccaatgcc tctgttcgcg ggaagcttgc
     9121 ctttcgattt gacccatttc tcgatctcgt tgatgcactg ctcgaggccg cagctggcga
     9181 tgaatgcctg gcccgggact tcgtagcctt ccgtgtggcc gttctttctt ctcacctcga
     9241 aggggtcgag gccgaggtcc gcgcacatcc agtcgatctg ctgctcggag cagaacatgg
     9301 cctggggggc accgaaaccg cgcatcgacg tcgagggcat ggtgttcgtg taaacacggt
     9361 agccgtcgta gcggtaactc tgccacttat acggaaagga gtgaaagaag cccgacaggt
     9421 agagggccgt tgcacccatg cccgtgtagg cgccgccgtt actgaacatg cgggactcgc
     9481 gggcaacgaa ggtgccgtcc ttcttcatgc ctgtcctgac gtagtagaac atgggggtgc
     9541 gcctcttgga ggcaatgaaa tcctcttcac gggtgagaac gatcttcacc ggcttatgga
     9601 gtttcatgga gaggatgccg ctgcagaact gggcggagtc tatctcgaac ttaccgccga
     9661 agcctcctcc gacatagggt gcgagaatgc ggacatctcc ctcgcgaagc ccaaagacgc
     9721 ctgccaggag ggcctggtgg tagtacgcgg actgcgtgga tgtccagacc gtgagcttgc
     9781 cttcaggggt gaaatttgaa acggcggccc gggtctccat gcacatatgg tgctggccgg
     9841 aacacttgaa ccagtcttcc ctgatgtagt cggcctgctc aaacccctcg tcgacatctc
     9901 cccactcgat gtgacgggtg acgttgatgt ttctttcgac gccctcgtgg atcgtgggag
     9961 cgtctttctt gatggcttcc agcggctcgt aaacagcggg aagaacttcg tattccacat
    10021 cgatgaggtc gagggccgct tccgcgatct cctccgtcac ggccgcgacg gccgcgacgg
    10081 gttcgccgat ataacggact ttttccaagg gaagtatctg ttcgtcgcaa agctcttcgt
    10141 agcgtctcca gatgccctgc tttatgccca gcgtgtcctt gccggtcacg accgcatgga
    10201 ccccggggag ttccatcgcc ctggtggtgt ctatgttgat gatcttcgcg tgaggatggg
    10261 ggctgcggag gatcttcccg tagagcatgc cggggagctt cacgtcgaat gtgtatgtgg
    10321 cccggcccgt taccttcgca atgccatcta tattgtggac cggcgtattg atgactgaga
    10381 attttttgtg actcatatta ctcctcctta tatttgccct gggcaacggc gtcaatggcc
    10441 cttacgatac tgttgtaccc tgtgcaccgg cagatattgc cttcgatgct ttcccggatc
    10501 tcttcctcgg aaggtttctt gtcccccatc tcattgagga acgccgttga ggacataagc
    10561 atgcccgggg tgcagaaccc gcactggtag gagccgttgt cgaggaacgc cctctggatg
    10621 gggctcagct tgccgttctc ctccaggcct tcgaccgtcg tgatatccat cccttcgcac
    10681 tggatggcga ggacggagca ggatttcacg gacatgccgt tcaccatgac cgtacaggcg
    10741 ccgcaggaaa ccgtgtcgca gcctctcttg gtgccggtga gacctacgtg ctcacggatg
    10801 gcgtccgcca gcaaggtctt cggatgtata aggatttcgt attcgtcacc gttaactcta
    10861 aaagtcaata cctgtttttt catatctctc ctccttaggc agccttctgg gcttgttcaa
    10921 aggccttctt cagcatcctc ttggtgagaa caccaagcac atgccttcta tgctcttcgg
    10981 atgcgtgaat atcggcgaca ggctgtgctt cctccgaagc gatctggccg gcctcggtga
    11041 agagcttgtc gtcaagcttc ttgcccttta aaaggtcttc ggccttcgtc actctaaggg
    11101 gtgtgggacc tgcgttgccg agaacgatcc gggcgttttt gcacgtgccg tcggcgttga
    11161 gcacaaccga ggcggcagcg gcaacgatac ccatctcgct gtcgaggaga ttgaatttct
    11221 gataagcaca accggatttc ggctccggtg cgggaacgtg cgcctcaaga acgagatcgc
    11281 catgacccat ctgcgtctcg aagtaatcga cgaagaactc gtcgagggga acggtctttt
    11341 cgccatcaac gttcccgaac tttatctccg cattgagggc gataagaacg ggtgcgaggt
    11401 cgcctgcggg atcggcatgg gcgaggttcc cgcctatggt tccccagttg cgcgtctgga
    11461 tcgacgcgag cttctgctcc atcgcaacga gggcaggata tttcttcgcg atgatatcgg
    11521 atttctcgac ctccctatgg gtcgtcgtcg cgccgatcct aagaccgtcc ttctcatcga
    11581 acttgatgta cttgagctca ttgacgtgct tgatatccac gagatattcg ggggcgagca
    11641 tgccctgcct caagacgatc aacaaggact ggccgccgca gatgactttg cagtcatcgt
    11701 gttccgcgta cattgcaagc gcttctttca ggcttgcagg cttcaggtac tgaaaatcgt
    11761 taatcatagg tctcctctcc tttctattgt ttctacttct tcaacgccga gccgagccaa
    11821 cgcttgccgg cagagatcag ttcttcttca aggcggcgaa ttcgcgctcg atgccgtatt
    11881 cctcgacatt cctgatgcac tgcgacccaa cctctatgtt gcccggcatc ccgaaggtct
    11941 tgtccgactc gtcggcggac tcctctttct tcaactcctt gatcctctcc ggatcgaagg
    12001 acgtaccccg ataggccggg gaatagcaca ggtaatagtg tcggccgcgg tagatagcgc
    12061 attccacgca agcctttccg gatatgggac ataatatttt gtgtttggcc ataggcagca
    12121 cccctgaata aagattggta atgcctgaaa agcattatgt ggcaaaatta ttgcggatat
    12181 ttgttcaaaa gtcaagttta tttttttccc ataatgaaca tatgtctgta ttgcataaaa
    12241 ttgcaaggtt ttattgcaca gcatttgttc atcatgatga actccgtgat tcttcgcccc
    12301 tgttttgtgt gcaattgtgt tgacaaatgg taccccgttt actataaaga gagaaaatca
    12361 catttcagga ggggttttat gaaacggttt ttcggggtaa agtgtttgct tttcgttttc
    12421 ttcgctgcaa cctgtcttgc cttctttcca gtaatggcgt cggcccagaa ggtcatcacg
    12481 ctcaactact ccaatttctt cccggcgccg cataagaaca gcgtcgtcgc gcagcagtgg
    12541 tgcaaggaag ttgagaagag gtcgggcggc aaggtcaaga taacctactt ccccggtggc
    12601 accctcaccc ccgccgcgca gacctatgac aacgtcgtga aaggcatcgc ggacatcggc
    12661 gagagctgct tcgcctacac caggggcaaa ttccccatga tggaagtcat cgaccttccc
    12721 ctcggctaca agagcggcgt ttccgcgacg aacctcatca acgcctacta cgccaagttc
    12781 aacccgaaag agcttgccga cgtcaaggtg ctctggcttc acgcacacgg ccccggcatc
    12841 cttctctcca agaaacccgt caagacgatg gatgacatga agggcatgaa gatccgcgcg
    12901 accggtctcg cggcgaaggt cgttcaggcg ctcggcggcg cacccgtcgg cacaacgatg
    12961 cctgagacct atgacgcgct ccgcaccggc gtggccgatg gcgccatggc tcccgttgag
    13021 gcggcacagg gctggaaatg gggtgaggtt gtcaagtaca taacccagaa cttcggctcc
    13081 gcctatacca ccggtatgtt cgtggtcatg aacaaggcaa agtggaacgc catgcctccc
    13141 gacgtccaga aggtcttcga agaggtcagc aaggaatggg tagtgaaaca gggtaaggtc
    13201 tgggatgaga tcgacaagga aggctacgat ttcgccaaga agagaggcaa tcaggtcatc
    13261 actctctcca aggaagaaga cgcgaaatgg gcaaaggccg tcaagcccct tcttgatgac
    13321 cacgtaaaaa ccctcaaggg taagggtctt cccggcgccg aggcagtcaa gtttgcggtt
    13381 gattatctga agaagaacta aacgaacaag atatagctaa aaaagggctc cgaaaccgga
    13441 gccctttttt tgtcgcaggt cgccagagat tgcggcgagg ggggtgggcc gtcacatttt
    13501 tgtggagcgg ccaaccgatt cttcttttgg cttctaactt gtgacctgaa acctgggact
    13561 tgtgacgatt ctaacacccg gccagggcaa ggaagttccg gaggagcgca aggcccacgc
    13621 gctggctttt ttcggggtgg aactgacagg cgaagaggga gcccgtctcg acggctgagg
    13681 tgaaagggcc gtcgtactcc gtggtcgtgg cgattacgga tccgtcgtcg ggggcgcagt
    13741 aataggaatg cacgaagtag aagaaatcgc ccgggtttat tcccttgagg atggggctct
    13801 ccttccggat gtcaacgctg ttccatccca tgtggggcac tttcatggag cccgtaaagc
    13861 gcggcacggt gcccttgatg accccgagac ccttgactcc cggcgcctcc tcgcttgatt
    13921 cgaagagcac ctgcatcccg aggcatatgc cgaggtaccg cttgtccctg cggatgtgct
    13981 ctctgatggc gggggccagg cccctgccct ccaggttctc catgcacctg ccgaaggcgc
    14041 ccacccccgg cacgactatg gcggaggccc cctcaatgga cgccgggtcg ccggacacag
    14101 tgacgccggc cccgagcttc gcgaatgcgt tgaggacgct tttcaggttg cccatcccgt
    14161 aatcgacaac aacgatcatt tacagtacgc ctttcgtgct gagtacgcct tttatgcggt
    14221 cgtccttctc caccgcggcc ctcaaggtcc gggcgaagga tttgaacacg gcctccacct
    14281 tgtggtggag gttgcttccg tagaggaggt tgacgtgaag ggtcagcctc gcctcgttca
    14341 cgaaggcctg gaggaattcc ttcaccacgt cgacgtcaaa tcctcccgtt cttccctgga
    14401 gacgcccttt gaagacgaag gaggaccgtc ccgatatgtc gacggccgtc atgcagagcg
    14461 tctcatccat gggaacgagc gcgctgccgt aacgccttat cccttccatg ccgccgaggg
    14521 cgtccctcag ggccttcccc atggcgatgc ccacatcctc cacggtatgg tggttgtcca
    14581 catcggtgtc cccttttgcc gtcacgacga ggtcaaagag gccgtgcttc gcgaagagat
    14641 cgagcatgtg gtcgaaaaag ggtatccctg tcgagatgct gtactcgccc gagccgtcga
    14701 ggtcccagga tacggagatg gacgtctctt tggtcgtcct ttccacttcg gccttcctcg
    14761 ccatatctcc cccttacatg atgacctttt tcaggggata ttccacgatc ccctgcgccc
    14821 cggccctttt caggtcgggg attatatctc tgacaacctt ttcgtcgatg atgacctcga
    14881 tggccaccca cttgtcgtcg gaaaggttcg ataccgtcgg cgtgtgcaga gagggaagga
    14941 tctccgtcac tctgccgagc ttgttcctgg ggatgttcat cttgagcccg accttctctt
    15001 ccgcaaggag cgcccccttc aggaggatga tgagattttc catcttcctc ttcttccagg
    15061 ggtccttcca agcggtcctg ttcgcgatga ggacggtctc ggattccaag atggtctcca
    15121 ctatcctcag gttgttggcg accagcgacg ctcccgtctc ggtcacctcc acgatggcgt
    15181 cggccagaag gggcggctta acctccgtgg cgccccagga gaactccaca gaggcctcga
    15241 tcccctgttt tttgagatac cgcttcgtga aggccacgag ctccgttgcg atcttcttgc
    15301 cctggaggtc cttcaccttc ttgatgggag aattgttggg cacggcgacg acccacttga
    15361 cgccgcggaa gcccaccttg ccgtagcgaa gacggacgag ctccaccacc ctggcatcct
    15421 gctccaggac ccagtcggcc cccgttatcc ccatgtcgag gatcccgtcc tccacgtagc
    15481 gggccatttc ctgggcccgt atgacgaggc cttccatctc gtcgtcgtct atgacgggga
    15541 cgtaggagcg ctcccggagc ttgatggtgt agccggcgtt cttgaaaagc cggaaagtcg
    15601 attcctgcag gctgcccttg ggcagaccga tcttcagttt catttggggg actcctttcg
    15661 tgcctttatt gcatcgccat gggcgggcag gccctctatc cttgcgagcc tttccgccct
    15721 gtcgccatat ttttcaatga atgtccggtc tattgtaaca acaaccttcc gctttgtaaa
    15781 ccggtcgacg gaaagccccc ctgtgaaccg tcccgcgccc gccgtgggca gaacgtggtt
    15841 cgtcccgata tagtagtcgc ccatggcaac aggcgtgttc agaccgacgt agatgacgcc
    15901 gggatagagt atcgagggca gacacgttgt gtcgcccagg agctccatgt gctccggcgc
    15961 gatgctgttt atcgcctcga cggcgcggcc gaggtcggtg tagtgcacca tgtacccgtt
    16021 gtcgtcgagg gccttcctga tcgtctccct gcgcggggca ccgtcgataa gcccttcgat
    16081 gctcttcatg acgtcgctga ggtgttcccg ggaagaggag aagagcccca ccgatgccag
    16141 ttcgtcgtgt tcggcctgtg agaacatgtc gagggccacc atcttaggcg agtgggcctc
    16201 ggtgcagaga acgacaagct ccgtcggccc ggcgagcatg tcgatgccga cggacccgta
    16261 aacgtccctc ttggcctctt ccacgtaggc gtttcccggc ccgacgatca tgtccacctt
    16321 cggcaccgag cccgtcccgt acgcgaaggc atatatggcc tgggcgcctc ctacgcggta
    16381 gacctcgtcg atgccgagga gtttcgcggc ggcggccacg tggtcattta tggcaccccc
    16441 ccggccgggc gtggtcgcgc agaccctctt caccccggct atctgcgccg gtaccacccc
    16501 catgaccagc gacgacgggt aggacgccgt tcccccgggg acgtagacca cggcgctttc
    16561 caccggcacc gggccttcct cgacgacgag gcccttcttc ctgaagaccc gtttcggcat
    16621 cttctgtccc ttgtggtagg aagtcacgtt cctgatcatc cccttgagga cggaaatgtc
    16681 ccttgccggg accctcgatg ccgcctcacc gatctcacgg gaggtcagtt tcagggggtg
    16741 cgccgccttc cagccgtccc aggcgatgga gaagtccacg agagcggcgt ctccgcggga
    16801 aaggacggcc tggcgtatct tcaggaccgc ctcgcggaca tcgcgcttct tgccttccct
    16861 gccggtcttc acgaaggata tgaggccgtc gaattcccgg tcaagctccc atactttcat
    16921 ctttcaccct ttcgatcctg gcgccgaggt ttttgagctt ttcttcgatg cgctcatagc
    16981 cccggtcgat gtggtatatg cgcgagacct ccgtcttgcc gtaggcggca aggcccgcga
    17041 tgatgagtga cgcgcttgcc ctcaggtctg tggccatcac cttcgcgccc gacatcgatt
    17101 cgaccccgtg gacgatggcc gtgttgccca cgaccctgat atcggcgccc atccgccgga
    17161 gctccgccgc gtgcatcatc ctgttctcga atatggtctc cgttatggcg ctcaccccct
    17221 tggaggtcgt catggccgcc atgagctggg cctgcatgtc cgtcgggaac ccggggtagg
    17281 gcacggtcct gatgtccacg gccaggggtc ttctcttcga catggacgcc ttgatcgttc
    17341 gcccgtcgca gtggatgtcc atgcccgtct ccgtgagctt gtcgataacg gccttgatgt
    17401 gctcgggaac acagttctcg aggacgatgc tcccccgcgt catgccgcag gcggcaagga
    17461 acgtccccgc ctctatcctg tcctctatga cgtcccaggc gcagggtgta agctcgccca
    17521 cgccgagtat ccgtatgacc gccgtcccct ctccttcgat gcgcgccccc atcttcttga
    17581 gcatccgcgc gagatcgacc acttccggtt cgcgggcggc gttctctatc agggtctccc
    17641 ccttggcgaa caccgccgcc atgaggaggt tctccgtccc gccgacggtg acgctgtcga
    17701 agacgatgcg cgccccttta agcttctttg ccgtgacatc gacataaccc tcccggatgg
    17761 cgacgtcgca gcccagcgcc gagaggccct tgaggtggag gtcgatgggc cgctccccta
    17821 tggcgcaccc tccggggtag gagaccgtcg ccttcctgag gccgccgatg agcgccccga
    17881 gaacgagaac cgaggcccgc atcttcttca ccagctcgta gggagcaacg tggttcccca
    17941 cctccgcggt gtctacctct accgtgtgct catcctgcca tacacacctc gcaccgagca
    18001 ggtcgagaag ctgcatcatc gtcgtgacat ccatgagacg gggcacgtta ctgatggcgt
    18061 acgtgcccct ctgaagcagc gtggcggcaa gaacgggcag gacggcgttc ttggagccgc
    18121 ttatcctgac tttcccctta agcctctctc cgccttctat aatgaactta tccatgtccc
    18181 gcgagcaccc tttccctgcc tgaatagtct tttctcactt tcaccctgag ccccgccacc
    18241 tcgagcatgg cgccgaccgt cccggcctgc gcttcggagc ctatctccag gaggaccgat
    18301 ccgcccgggg caagacgccg gggcagctcg gcggcgatcc gccggtacac ctcgagaccc
    18361 ccttccccgc cccgcagggc acgggctggc tcgaaatccc tcacatcggc cataagctcg
    18421 tcccactcgt cgtcggccac ataggggggg ttcgacagga ccatgtcgaa gcgtgtcccg
    18481 tcgctgaatg ccgcgaaaag gtcgccgcac acaaagcgcg tcctttcttc gaccccgatg
    18541 gagcgggcat tcctgcgcgc caccgcgagg gccgcctcgg atatgtcgac acagacgacg
    18601 ctgctgcccg cgtgtttcgc gataacgatg ccgatcgccc cggaacccgt ccccatgtcg
    18661 agaatcctga ggtccctcct gccgccgatg agggcgaggg cctcctccac gaggacctcc
    18721 gtctccggcc gcgggatcag caccgcctca ttcacggaga aggtctcgga aaagaactcc
    18781 cgcgttcccg tcatatacgc caggggcctg cccgcagcgc gctcgtcgag ggccttccgc
    18841 acggcgccga ggtcttcccc gccgatctca cggtccatgc ggcagaagag ctgttccttc
    18901 gtaaggccga gggcgaggca tacggttgcg atgacgtccg tcctggagag acccctctcg
    18961 cctgcgatga tctggctgat cctcaatgcc ctttaccctt ttttgagtgc ttcggagcgg
    19021 aaatgcgcgg tcacgggacc cgttatctcc tctatgttcc cgttgaggat gtcctggagt
    19081 ttgtacaggg tgaggccgat cctgtggtcg gtgacgcgtc cctgggggaa gttgtaggtc
    19141 ctgatgcgct cgctgcgctc gccggttcct acctgttttc gccgctcgtc ggagatctcc
    19201 tgctccttct cgccttccat cttttccttg agccgggccc tgaggacgcg catggccttt
    19261 gccttgttct tgtgctgcga tttctcatcc tggcacgtga cgaccatgcc tgtcgggatg
    19321 tgggtaagcc tcaccgccga gtcggtggtg ttcacgtgct gccccccggg cccgctggaa
    19381 cggaagaggt cgacgcgcac ctcgtcggga ttgatggtga cctccagctc ctccggctcg
    19441 gggaggacgg caacggtgat cgtcgaggtg tggatccttc cctgggcctc cgtctcgggg
    19501 accctctgca cgcgatggac gccgctctcg taacgcaggg tattgaaggc gtcctttccc
    19561 tcgatgacga cgatcac
//
LOCUS       DAOQTR010000002        14626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_4646_length_14626_cov_4.802553, whole genome shotgun sequence.
ACCESSION   DAOQTR010000002 DAOQTR010000000
VERSION     DAOQTR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 14626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14626
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4646_length_14626_cov_4.802553"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKN85_00110"
     CDS             <1..414
                     /locus_tag="PKN85_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42833.1"
                     /translation="LMQYSWPGNVRQLESVLERSYIMCEAGTIGTEYVPAEVRHYSQP
                     RAAIDGITSRLDELINAKLTAAEYRLYLYLTKLDPRDGASDHLLEPKEIAERLGISRD
                     TFFVGVSKLKELGLYDYKARVNELKHPPGRSPRKE"
     gene            599..1051
                     /locus_tag="PKN85_00115"
     CDS             599..1051
                     /locus_tag="PKN85_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42834.1"
                     /translation="MKKALMIVLAILITVAFVTTVMAQDKKAATDKPAAATDKPVKEK
                     VAEKAKKAGVKKFAGDFVSMDEAAKTIVVKGETEMTFDVSKVKKMAKFAAGDKVNVVY
                     TEADGKNVAKAVTKAGAVKEKAKEKKEKKAEEKKDEKPAEKPAAPAKK"
     gene            complement(1152..3119)
                     /locus_tag="PKN85_00120"
     CDS             complement(1152..3119)
                     /locus_tag="PKN85_00120"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42835.1"
                     /translation="MIADVALPIPVGRSFSYRVPGELAPHIFELGRVLVPFRYREAAG
                     VVIALREGEEEGLKSVAGVLDLFPLLSDELPALLRWSSSFYVTPAGIAFKHALPSAGD
                     LERFLSVESAGLPEVDGLPLRKAIRRLGRAGLLQLYREGALSLCETLTGKTFAPPPTA
                     PPGLGEIQREKALLIDSVEERLAYYNRVIPDHLGQGRNVLVLVPDYHAAGAWFAKRLR
                     EAYGPRVLWFSSGTPVKQRMETWFRVRREGGHVVLGNKNCVFLPLHDLSLVIVERCDD
                     DEYKNEEGLKFNAVRVAVERARLKGASIVLGCAASSIDAIHLARADGFRISCNEWPTR
                     GGYAERTTNVSSPSTGEIIDHLGAEAREAARQGGRVAIYTPRKDQGSYLKCHACRETL
                     SCPDCGEALTYDSKAGSLACPACRARLAYEGICPQCGSSAVGFSRIGVHFIEEHLRAA
                     LPDLSVTRITGDSLKKEIAAIRRQPQGAGSVLVGTQSLSKLYSLSIDRLLLAQWEELR
                     KMGGYRSEERTHQVLLNLLDALTPRSIISYSTRKEPADPREYLDISSFAERELRKRRD
                     AQFPPFVRVFLLEARAKTPARADAILKKVRTIIADGALEGALLGTLPLERPPYHIRKV
                     ILKGDEDLLRDTFEKFHDIPGVDIEADPTSF"
     gene            complement(3116..3940)
                     /gene="murI"
                     /locus_tag="PKN85_00125"
     CDS             complement(3116..3940)
                     /gene="murI"
                     /locus_tag="PKN85_00125"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNT42836.1"
                     /translation="MQKLAIGMFDSGVGGMTVLREVRELLPREHIIYFGDTARVPYGN
                     KSSRMVTKYALESALFLLTKGIKLLIIACNTSSAFALPILQRRMPVPVIGVIGPGAKE
                     AVRNTRNGRVGVIGTRTTIKSMAYDRSIKKLDPGIEVHSRACPLFVPIVEEGLEHDEI
                     AGLMARKYLGALKGSGIDVLVMGCTHYPVLEDVIRKTMGKDVKIVHSGKETAKEARAI
                     LEEKDLFNRRGRGKCEYFVTDAPESFHEIGGRILGEPLTHVRSLKGLDYRDLLFSS"
     gene            4178..5407
                     /locus_tag="PKN85_00130"
     CDS             4178..5407
                     /locus_tag="PKN85_00130"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNT42837.1"
                     /translation="MTRIGIACDGGRGRAISGDALVRRAMASGGVTRPDLVLAFCSGR
                     NEPEAFFRGIRRVAGAHVPVVGGSAIGVISNDFLSYRDPVAAVAVLESDLIAFRLASA
                     GQVNENEREAGRKVAAAIARGPEDRLLLVFYDSIKVPPEEGSPPIMNSSTPLIAGIGE
                     VLEDAVPVIGAGLLGDYGFNRTVQFRGSSMASQSLVALMLGGDLSVYHRITHGCAPLD
                     GVYRRVTRVQGPLLYEIDGKPIVEMINELFDSEEWQEERPLDYLTLGVHCGERYERYR
                     ENQYVNRLILGVIPDRSGVMMFESDLKEGDEVQFMVRDAGRMVESALNNSRLLVDMID
                     KDGKVPLLALYIDCAGRAKDYSNTSVEEAGQVQKVLSERNIPLFGFYSGVELAPLLGR
                     TRGLDWTGVLTILARKP"
     gene            5413..7461
                     /locus_tag="PKN85_00135"
     CDS             5413..7461
                     /locus_tag="PKN85_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT42838.1"
                     /translation="MERDVLKEKIVAYFRGIAEDRGRKYLREVSKLSRIIAKARKTEE
                     FLGRYRVLVESFSDAIFMLDEDRKIISCNRAFRSLFQLKKDEIEKKTLRIITGGDDAC
                     DRLTATIDDIIAGNNRSVFSWEFVKKDGSSLTMEVTVSQVRASLSEEAAPIGGCVAIL
                     HDITDRIAREDALRAAEEKYRNIFENAAEGIFQVAVDGHYISANPALARIFGFDSPLQ
                     MTGDGDLRARKTFVDPEDYRRLNAILKARGFAENFESERYRTDGTRMWTSTNVRIVTD
                     RDNGTHYFEGTVRDITRRKNLESQLRHSQKMEAIGTLAGGVAHDFNNLLTALIGYGNL
                     LQVKMEKDNPLRVYVDHVLSSAKRAANLTNSLLAFSRKQAVNLEPNGVNEIITGVQRL
                     LERLLSEDIELHVQTSAREVTIMADRTQIEQILMNLATNARDAMTDNRVLTISTAHAS
                     IDKAFIHKHGYGKVGTYGRVSVTDTGHGMDENVKEHIFEPFFTTKEAGKGTGLGLSTV
                     YGIVKQHDGYITVESEPGKGTTFHLYFPSVRVKREKARQVESKMPSGTETILVAEDDR
                     ETLQLMKEVLKEYGYTVIGAADGDEAVRHFTEHRHRIGLVIIDVIMPKKNGMEVYSEI
                     SKLDPSVKVLFVSGHTRDVVLDRGVVDSRSNFLSKPIAPSLLLERLRTLLDTEAHSPL
                     "
     gene            complement(7582..8919)
                     /locus_tag="PKN85_00140"
     CDS             complement(7582..8919)
                     /locus_tag="PKN85_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNT42839.1"
                     /translation="MAMERKGPVRPPTEKGTPQAVPERPLGGREAFFHRVHEELFGGP
                     SVLCVSARVAGPLDIQLLKHACHRVWERHPVLKARIVSREGKPSFLFNVPFRDIPIHS
                     FFELGRTNFHTVLEREVDTLFDVSKHLWRVLLITDKTDLKRHYLLLSLHHSISDGTSA
                     VRLAEELISCCTRILAGETPRTDPLPLRASLEELMASAGGTSRQSGTATGRPPGESGG
                     GSMPYHEFQPPGRRHTRFRVHTMEVAETAVLEEKRLRAGTSIHSALAASALTVMRKHF
                     GDDVRLSVTTPVNARGLTGTAIGDGELWCMETPVSIHGEDIGGRDTWQMARDYSDKLA
                     FATAGGMALSACANTDPAEEIEGLRAARHFPLSCLLLEEGRVGPDQKSPFVVENMGLV
                     SGRQAADHIMAISAATAGDALSLTFAYTSPLLRESWADRFVSEFLHNLERMAK"
     gene            9060..10076
                     /locus_tag="PKN85_00145"
     CDS             9060..10076
                     /locus_tag="PKN85_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT42840.1"
                     /translation="MGEVRAMVIPVFLPHSGCNERCVYCHQGYITDSGGADIKARIDG
                     ALRDRREACEVGLFGGNIFGMEPADLERLFSFFAPYKDVVKGFRLSTKPVPLRDDAID
                     ILKRNGVRMIELGIPTFNDAILAALNRAHTAKDLFRAFDRLLGEGFRLALQFMVGLPG
                     ETMVDIEETVENMVRLRPEYIRIYPLVILRNTPLYRLHETGGFTPIAFDEALERAALI
                     YRRALEEGIDVVNVGLTDNELVREMVAGGFYHPAFGSLVQSRIFRDALGSVLGEIEGP
                     KEVTVVLHRNDIPLLVGYRRENLKHFASLGIALRWEPSGAERGRFEVVSGGERVTGRM
                     IQRR"
     gene            complement(10142..11035)
                     /locus_tag="PKN85_00150"
     CDS             complement(10142..11035)
                     /locus_tag="PKN85_00150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT42841.1"
                     /translation="MKEQPLVSIIVPAYNYGTYVHRAIGSCLRQSSGNLEVIVIDDGS
                     TDATGEIVRKIADQRVVYHYQENQGVSAARNKGLRMAKGDFIAFLDADDYLTDDSVET
                     RVTVMLENPDIDFVTTTASSVDDAGRVSFRPYRGPRDIVSRELCQALLLKRIPYTTSA
                     VLVRGSHARKFEFATDLNNGEDLVFFSKVFFDRKGCFLSKPTAVTFSHPDSLRHNIAN
                     LRTQGAGLIEAIFDDPYFAGRLEHIRRPFAANRYVELFRRFYRSGDDKLAREYYRKAL
                     ALEPSRIFKIDHLFKFIRTWF"
     gene            complement(11042..12163)
                     /locus_tag="PKN85_00155"
     CDS             complement(11042..12163)
                     /locus_tag="PKN85_00155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT42842.1"
                     /translation="MKIGLAIYNFDPKKGGAERYTFDLARRLAGKGHEICVFCGRGVE
                     TEGVRLIRLRTNSFPRWRRNLSFALTHRQALDRIRPDIMLGYGNILDLDVYQSHGGVQ
                     SVWMEREIVSYGPGGAGMKAFLLRNSLNQAIQRWIEGYSVKKHRFTRIVAISDMVRDH
                     MSAHYGIDRNTFDVVYNGVDTDRFKPAPERPAGPTTILFCAGNFRLKGLSPLIAAMGR
                     AVEKGKDVRLTVMGRGKKERYRGLIDAAGIADRVTFTGERSAPEEVYREAHVLAHPTY
                     YDACSLTTMEGMASGLPIITTRWNGACAFVSPDEGFVIDEADNIEALSAAICALTDPV
                     RQEVMGRNARKKMESFTMERNADEMEAVLLGAFEEKGRR"
     gene            complement(12205..13338)
                     /locus_tag="PKN85_00160"
     CDS             complement(12205..13338)
                     /locus_tag="PKN85_00160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT42843.1"
                     /translation="MKVLHLFSDWKWTGPAEPVLSLCENLGNEGVDVVLACRKSPADF
                     RERTVDREAGKRGLRCYEGLRLNRYFSARDWWHDLISFNPYVLKEKFDIVHTHLSHDY
                     YTAVTALSFSRARPLLVRTDHKRDGLPANIFMAWAMARTDGLVTYSERLAARDRESFA
                     YPGERLRVIPPGVKPYEGPLKDLRAELGIAPDERVIGVIGRLKPDRGYDVILSAFGML
                     RSRVDRVKLVIVGRSSQIETSVMKPLKELGIEKDVVIAGYRIDDYFSMIHTFDLYIMM
                     RAGSDGSGRALREVLSMGRPAIVSDRGMLPDLVEDGRTGYVTTWDAADLAGKMELLVS
                     DAARRRAFGENARNRADSEWSFRLQAKAMKQFYDFLLTSGRRA"
     gene            complement(13335..14444)
                     /locus_tag="PKN85_00165"
     CDS             complement(13335..14444)
                     /locus_tag="PKN85_00165"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT42844.1"
                     /translation="MNVRIFDIEKDHAGIKAELVRTFEKVLSSGEYILGREVKSFEEA
                     FAACVGTRYAVGVASGTDAIKIGGLALGLTEGDKFVTTPNTYIATAMALSEGGLVPRL
                     CDIEADTYNMDPAALEELLGKEKDIKLCVPVHLYGQPCRMDEIRDICERHGVRVMEDA
                     CQAHGSRYKDRNAGTMSDVAAFSFYPTKNLGCYGDGGIVVTDREDVFRRMLMLRAHGQ
                     SDRHVHDIQGFVSRLDELQAALLSVKLPHLDGWNEGRRRNATLYDRGLEGLPVRRPVT
                     VPQAFHVYHLYVIAVEEREALRAYLSEKGVTTLVHYPTPIHLQKVYSYLGYGEGSFPK
                     AEKASRQIISLPVHPSLREEEIGFVCRTIGEFYGR"
BASE COUNT         2902 a         4198 c         4565 g         2961 t
ORIGIN      
        1 ctcatgcagt attcctggcc gggtaacgtc cgccaactgg aatcggtgct ggaacgttcc
       61 tatataatgt gcgaggcggg gaccatcggc acggagtatg ttcccgccga ggtgaggcat
      121 tactcgcagc cccgcgcggc catcgacggg ataacgtcga ggctcgacga gcttatcaat
      181 gcgaagctga cggcggccga gtatcgcctc tacctgtacc tcaccaagct ggatccccga
      241 gacggagcgt ccgaccatct cctcgagccg aaagagatag cggagcgcct gggtataagc
      301 cgggacacct ttttcgtcgg cgtgtcgaaa ctcaaggagc tcgggctcta cgactacaaa
      361 gccagggtga acgaactcaa gcaccccccg ggccggtccc ccaggaagga atagtcggat
      421 atttccgatt caaagggcat tccggagtcg gatatttccg attatcgccg cttccccgaa
      481 agacactcca aacctctttc ctttaatatc aatctgttgc gatagtggca cgacaactgc
      541 atacacagtc cccatgcaac tcaatgtgac caacaaaagg ggaggaggtg aaaatagcat
      601 gaagaaagca ttgatgatcg ttctcgcgat cctgatcacc gttgccttcg ttacgaccgt
      661 tatggcgcag gataagaaag ccgcgacaga caaaccggcc gcagcgacgg acaagcccgt
      721 gaaggagaag gtggccgaga aagccaagaa agccggcgtg aagaagttcg caggcgactt
      781 cgtctccatg gacgaggcgg cgaagacaat agtcgtcaag ggtgagaccg agatgacctt
      841 cgacgtttcc aaggtgaaga agatggcaaa gttcgcggct ggcgacaaag tcaacgtagt
      901 ctacaccgag gcagacggca agaacgttgc caaggcagtg acgaaagccg gcgcagtgaa
      961 ggaaaaggcc aaagagaaga aagagaagaa agcggaagag aagaaggacg agaagccggc
     1021 tgagaagccc gcggctccgg ccaagaagta gttccgcatc gcgaacagaa aaaagggagg
     1081 ccaaaagcct cctttttttt ggttcttttc gctcaagcca ctcaaacggc tcaagcggct
     1141 caagcggtct ttcagaagct ggtcgggtca gcttcgatgt cgactccggg gatgtcgtgg
     1201 aacttttcga aggtgtccct gaggaggtct tcatctccct tgagtatcac cttgcgtatg
     1261 tgataggggg gcctttcgag gggtaaggtg ccgaggagcg cgccttcgag ggctccatcg
     1321 gctatgatcg tgcggacctt cttgagaatg gcgtcggccc gggcgggggt cttggcgcgg
     1381 gcctcaagga gaaagacccg gacaaatggc gggaactgag catccctgcg tttcctgagt
     1441 tcccgttcgg caaatgagga aatatcgaga tactcgcggg ggtcagcggg ctcttttctg
     1501 gtggaatagg agatgatgga ccggggtgtg agggcgtcga ggagattgag aaggacctga
     1561 tgggtccttt cttccgatct gtatcctccc atctttcgca gttcctccca ttgtgcgagg
     1621 aggagtctgt cgatggagag gctgtagagc ttggagaggg attgggtacc gacgaggacg
     1681 gagcctgcgc cctggggctg tctgcggatg gcggctatct ctttcttgag ggagtctccc
     1741 gttatgcggg tgacgctgag gtccggcaag gcggcgcgca ggtgttcctc gatgaagtgt
     1801 actcctatcc gggagaagcc gacggcgctg ctgccacact ggggacagat gccctcatag
     1861 gccagccggg cccggcaggc gggacaggcg aggctgcccg ccttgctgtc gtaggtgagg
     1921 gcttccccgc agtccggaca ggaaagggtc tccctgcagg catggcattt gaggtacgag
     1981 ccctggtcct tgcgcggtgt gtagatggcg acgcgcccgc cttgccgggc cgcttccctg
     2041 gcctcggcgc cgaggtggtc gatgatctcg cccgtcgaag gggaggagac gttcgtcgtt
     2101 ctttccgcat agcctccccg ggtgggccat tcattgcaac tgatccggaa accgtctgcc
     2161 cgggcgagat gaatcgcgtc gatggaggag gcggcacagc cgaggacgat ggaggccccc
     2221 ttgagccgcg ccctttcgac ggcaacccgc acggcgttga acttcaaccc ctcctcgttc
     2281 ttgtactcgt catcgtcgca tctctcgacg atgacgagag agaggtcgtg aagagggaga
     2341 aagacgcagt tcttgttccc gaggaccacg tgcccgccct cgcgacggac cctgaaccag
     2401 gtttccatcc tctgcttcac gggcgtaccg gaggagaacc acagaacacg gggaccatag
     2461 gcttccctga gtctcttcgc gaaccacgcc ccggcggcat ggtaatcggg gacaaggacg
     2521 aggacgttcc tgccctgccc caggtggtcc ggtatgaccc tgttgtagta cgccagccgt
     2581 tcttcgacgg aatcgatgag aagggccttt tccctttgga tctcccccaa ccccggggga
     2641 gccgtggggg gcggggcaaa ggtttttccc gtaagggtct cgcaaagact gagtgccccc
     2701 tccctgtaca gttgcaggag tcccgccctg ccgagtctcc tgatggcctt cctgaggggc
     2761 agaccgtcca cttcggggag gccggcggac tcgacggaga ggaagcgttc gaggtctccc
     2821 gcggagggga gggcgtgttt gaacgcgatc cctgcgggcg tgacatagaa tgacgatgac
     2881 catcggagca atgccggaag ctcgtcggag agaaggggaa aaagatcgag cacacccgcg
     2941 acggacttga gcccctcctc ttcgccttcc ctgagggcga tgacgacgcc ggcggcctcc
     3001 ctgtaccgga aaggaacgag gacacgcccg agttcgaaga tatggggggc gagttcaccg
     3061 gggactctgt aggagaagga cctgccgacg ggtatcggga gggctacatc ggcgatcatg
     3121 aagagaaaag gagatcgcgg tagtcgaggc ccttcaggga cctcacgtgt gtcagcggct
     3181 caccgaggat cctgccccct atctcgtgga acgattcggg ggcgtccgtg acaaagtact
     3241 cgcacttgcc gcgcccccgt ctgttgaaga ggtctttctc ttcgaggatg gcgcgggcct
     3301 ccttagcggt ttcctttccg gagtgcacga tcttcacgtc cttacccatg gtcttcctga
     3361 tgacgtcctc gaggacgggg tagtgggtgc atcccatgac gaggacatcg atcccggagc
     3421 ccttgagggc gccaaggtac tttcgggcca tgaggcctgc tatctcatca tgttcgaggc
     3481 cttcctccac tatgggaacg aagagggggc acgcccggga gtggacctct atgccgggat
     3541 cgagcttctt gatcgaccgg tcgtaggcca tgctcttgat cgtggtcctc gtgccgatga
     3601 ccccgaccct cccgtttctg gtgtttctca ccgcctcttt ggcgccgggc ccgatgacac
     3661 cgatgacggg aacgggcatc cgtctttgca gtatcgggag cgcgaatgca gacgaggtgt
     3721 tgcaggcgat gatgaggagc ttgatgccct tcgtgagaag gaaaagggcg ctttccaggg
     3781 catatttcgt caccatccgg gaggacttgt tcccgtaagg aaccctggcg gtatctccga
     3841 aatagatgat gtgttcgcgg gggagaagtt ccctgacctc cctgaggacg gtcatgcctc
     3901 ccaccccgga gtcgaacatg ccgatggcca gtttttgcat ggctacagat taggagagat
     3961 gggggtgaaa tgtcaaggca ataagcccgc gggcagagga gcctttttgc ttgccttttt
     4021 aaatccaatg ctggtaaata gaataaagcg attccctgac aatctgcctt gtgtaacagc
     4081 tgtggaccgc ggaccgccac tgtagtgccc ccgtgtcgag ggggaatgtg acgccccggg
     4141 ggacggcacg ggaaacaaag gggttgaaac ggagacgatg acgaggatag gtattgcctg
     4201 cgacgggggc aggggccggg ccatttccgg agatgccctt gtcaggcgcg caatggcgtc
     4261 gggcggcgta acgaggcccg acctggtcct cgccttctgc tccggcagaa acgaacccga
     4321 ggctttcttc cgcggtataa gacgcgttgc gggagcccac gtccccgttg tcggagggtc
     4381 cgcgatcggc gtgatatcca acgactttct ctcctacagg gaccctgtgg cagcggtggc
     4441 ggtgctcgag tcggacctca tcgccttcag actggcttcg gccgggcagg taaacgagaa
     4501 cgaaagagag gccggcagga aggtcgccgc cgctatcgcc cgcgggccgg aagacaggct
     4561 tctccttgtc ttctacgatt cgatcaaggt tccacccgag gaaggatctc ccccgatcat
     4621 gaattcatcg acgccgctca tagccgggat cggagaggtc ctggaggatg ccgttcccgt
     4681 catcggggcg ggactcttgg gtgattacgg tttcaacagg acagtccagt tccgcggttc
     4741 ctcgatggca tcgcagagcc tcgtggctct catgctgggc ggcgatctct ccgtctacca
     4801 tcggataacg cacggctgcg ccccactcga cggggtctac cgcagggtga cgagggtgca
     4861 gggcccgctc ctgtacgaga tagacggcaa acctatagtg gagatgataa acgaactctt
     4921 cgacagcgag gaatggcagg aggagcggcc ccttgattat ctcaccctcg gggtccactg
     4981 tggagagaga tacgaaaggt atcgggagaa ccagtacgtg aacagactga ttctgggcgt
     5041 cattcccgac aggtcagggg tgatgatgtt cgaatccgac ctcaaagagg gtgacgaggt
     5101 ccagttcatg gtacgggatg ccggccggat ggtggagtcc gctctgaaca attcccgtct
     5161 cctcgtggac atgatagaca aggatggaaa ggtacccctt cttgccctgt acatcgattg
     5221 cgcgggccgg gcaaaggact actccaacac gagtgtcgag gaggccggcc aggtgcagaa
     5281 ggtcctcagc gagcgcaata tccccctttt cggtttctat tcgggcgtgg aactcgcacc
     5341 gctgctggga agaacgagag gcctcgactg gacgggggtt ctcacgatcc tcgcgaggaa
     5401 accctagtgt ccatggaacg ggatgtcttg aaggaaaaga tcgttgccta cttcaggggc
     5461 atcgccgagg acagagggag gaaatatctc agggaagtaa gcaagctatc gcgcatcatc
     5521 gcaaaggcca gaaagacgga agagtttctc gggcgctacc gggtcctcgt cgagagcttc
     5581 tccgacgcga tattcatgct cgatgaggac aggaagatca tctcctgcaa ccgcgccttc
     5641 cgcagcctct ttcaattgaa aaaggacgag atagaaaaaa agacactgcg catcatcaca
     5701 gggggtgatg acgcgtgcga ccgccttacc gcgaccatcg acgatatcat cgcggggaac
     5761 aacaggtctg tcttttcctg ggaattcgtg aaaaaggacg gctcttccct caccatggag
     5821 gtgacggtat cacaggtgcg cgcttcgctc tccgaggagg cggcgcccat cggcgggtgc
     5881 gttgccatcc ttcacgacat cacagaccgc atcgcgcggg aggacgcgct cagggccgcc
     5941 gaggagaagt accgcaatat cttcgagaac gcggcggagg gcatctttca ggtcgccgtc
     6001 gacggccact acatcagcgc caacccggcc ctcgcgagga tcttcggatt tgactcgccc
     6061 cttcagatga cgggggacgg ggacttgagg gcaaggaaga cattcgtcga tcccgaggac
     6121 taccggcgcc tcaatgccat cctgaaggcc cggggattcg cggagaactt tgaatcggaa
     6181 cggtaccgca cggacgggac caggatgtgg acatcgacga acgttcgcat cgtgaccgat
     6241 agagacaatg gcacccacta ctttgagggg actgtcagag acatcacccg ccgcaagaac
     6301 ctcgagtccc agctgcggca ttcccagaaa atggaggcca tcggcacgct ggccgggggt
     6361 gtcgcccacg atttcaacaa tcttctcacc gccctcatcg gatacggcaa cctcctccag
     6421 gtcaaaatgg agaaggacaa cccgctgcgc gtctacgtgg accatgtcct ctcgtcggca
     6481 aagagggcgg cgaacctcac gaacagtctc ctggccttca gcagaaaaca ggcggtgaac
     6541 cttgaaccca acggcgtcaa cgagatcata acgggggtgc agaggcttct cgaaaggctt
     6601 ctcagcgagg acatcgagct tcacgtgcag acctcggcca gggaggtcac cataatggca
     6661 gaccggacgc agatcgagca gatcctcatg aaccttgcca cgaatgcccg cgacgccatg
     6721 acggacaacc gcgtcctgac catctccacc gcccatgcgt caatcgacaa ggcctttatc
     6781 cacaagcacg gttacggcaa ggtgggaacc tacgggcgtg ttagcgtgac cgatacgggc
     6841 cacggcatgg acgagaacgt caaggagcat atattcgagc ctttcttcac aacgaaggag
     6901 gcgggcaagg gcacgggact ggggctttcg acggtgtacg gcatcgtgaa gcagcatgac
     6961 gggtacatca ctgtggaaag cgagccggga aaggggacga cgttccatct gtacttcccg
     7021 tcggtgcggg taaagaggga gaaggcaagg caggtggaat cgaagatgcc ttcaggaacg
     7081 gaaacgatcc tcgtggcgga ggacgatcgg gagaccctcc agctcatgaa ggaggtgctc
     7141 aaggaatatg gctacacggt tataggtgcc gctgacgggg acgaggcggt ccggcatttc
     7201 accgagcaca ggcacaggat cggacttgtc atcatcgatg tgataatgcc gaaaaagaac
     7261 ggcatggagg tctattccga gataagcaaa ctcgaccctt ccgtcaaggt ccttttcgtc
     7321 agcggtcaca cccgggacgt ggtgctcgac aggggtgtcg tggacagcag gagtaacttt
     7381 ctctccaagc cgatagcgcc ctccctgctg ctggagagac tgcggaccct tctcgacacg
     7441 gaagcgcaca gccctctcta gcattcgtcg aagatcctcc ggacctcggc cgggccagag
     7501 accccccttg accgcaactg gcgttcaggt ccgatcccag ccctattgac cctgctcaaa
     7561 cccaccgccg cctgtccttt tttattttgc cattctctcg aggttgtgca ggaattcgct
     7621 gacgaacctg tccgcccagc tttccctgag gagcggagat gtgtaggcga aggtcaggga
     7681 gagggcgtct cccgccgtcg cggcggagat ggccatgata tggtccgcgg cctgtcgtcc
     7741 gctgacaagg cccatgttct cgaccacgaa aggacttttc tgatccggtc cgacgcgtcc
     7801 ttcttcaagg agaagacagg aaagagggaa gtgccgggcc gcacgcagcc cttcgatctc
     7861 ttccgccggg tctgtgtttg cgcaggcgga gagggccatt cccccggccg ttgcgaaggc
     7921 cagtttgtcg ctgtaatccc gggccatctg ccacgtgtcg cggcccccga tgtcttcacc
     7981 gtgtatgctg acgggtgtct ccatgcacca gagctcgccg tcgccgatcg ccgtcccggt
     8041 cagacccctg gcgttgaccg gggtggtcac ggagagacgg acgtcatcac cgaagtgctt
     8101 gcgcatgacc gtaagagcgc tcgccgcgag agcggagtgg atactcgtcc cggcgcggag
     8161 gcgcttctct tccaggacgg ccgtctcggc cacctccatg gtgtggacgc gaaaacgggt
     8221 gtgccgcctg cctggaggct ggaattcgtg gtagggcatg gagccgccgc cgctttcgcc
     8281 ggggggtctc cccgtggccg ttccggactg cctgctcgtg cccccggcgg acgccataag
     8341 ctcctcgagg gacgcacgga ggggaagagg gtccgtgcgc ggggtctcgc cggcgaggat
     8401 cctcgtgcag caggagatca gctcctcggc caggcggacc gcggaggtcc cgtcacttat
     8461 cgaatggtgc agggacaaga ggagatagtg tcgcttgagg tcggtcttgt ccgttatgag
     8521 aagaaccctc cacaggtgtt ttgaaacatc gaaaagggtg tccacttcgc gttccaggac
     8581 agtgtggaaa ttggtcctgc ccagctcgaa gaacgaatgg atggggatgt cgcgaaaagg
     8641 tacgttgaag agaaaagaag gttttccttc cctggaaacg atgcgtgcct tgagcaccgg
     8701 gtgacgctcc cagacgcgat ggcaggcgtg tttcaagagc tggatgtcga gaggtcccgc
     8761 aacacgtgcc gatacgcaca gtacggaagg tcccccgaac aactcctcgt ggacacggtg
     8821 aaagaaggcc tcgcgaccgc ccagcggccg ctcgggaaca gcctgcgggg tacctttttc
     8881 ggtgggcggt ctcaccggtc ctttgcgttc catcgccatg gttcatccat tgtgctgtga
     8941 aagacgcatt tttgcaaccc ttttcagtat gacagccggc aaggtgcgag gcataccggt
     9001 ttcccggtcg tgcccgcgtt cataacgatt tgcttttttg gctcaaaaga gtggacaata
     9061 tgggcgaggt ccgcgcgatg gtcatacccg tgtttcttcc ccattcaggc tgcaacgaac
     9121 gttgcgttta ctgtcatcag ggctacatca ccgacagcgg gggcgcggat atcaaggcga
     9181 ggatcgacgg cgcgctcagg gacaggcgcg aggcctgtga ggtgggcctc ttcgggggca
     9241 acatcttcgg gatggagccc gcggacctcg aaaggctttt ttccttcttc gccccttaca
     9301 aggatgttgt aaagggcttc cgcctctcga cgaaacccgt tccgctccgg gacgacgcga
     9361 tcgacatcct caagcgcaac ggcgtccgca tgatcgagct cggcattccc accttcaacg
     9421 acgccattct tgccgccctt aacagggccc acacggctaa ggacctcttt cgcgcctttg
     9481 acaggctcct cggggagggt ttcaggcttg cccttcagtt catggtgggc ttgcccggcg
     9541 agacgatggt tgacattgaa gagacggtgg agaacatggt gaggctgaga ccggaataca
     9601 tccgtatcta tcccctggtg atcctcagga acacgccctt gtacaggctt cacgaaacgg
     9661 gagggttcac ccccattgcc ttcgacgagg ccctcgagag ggcggcgctg atctaccgta
     9721 gggccctgga agagggtata gacgtggtca acgtggggct cacggacaac gaactggtca
     9781 gggagatggt tgccgggggg ttctaccatc ccgcttttgg cagtctcgtg cagtcacgca
     9841 tattccgcga cgcccttgga tccgttctcg gggagatcga gggcccgaag gaggtcacgg
     9901 ttgtcctcca caggaacgac atacctctcc tcgtaggata caggagggag aacctgaagc
     9961 atttcgcgtc tctgggtatc gcccttcgct gggagccgtc gggggccgaa cggggccgtt
    10021 tcgaggttgt aagtggaggg gagagggtga cggggagaat gatacagaga agataatgac
    10081 caaattattg gtcattatct tcttctttgg tcattgtgat tcggtccttg gttattttct
    10141 ttcagaacca tgttctgatg aatttgaaaa gatggtcgat cttgaatatc ctcgagggtt
    10201 cgagagcgag ggccttccgg tagtactccc gagcaagctt gtcgtccccg gaacggtaga
    10261 accgccggaa caactccacg tagcggttgg cggcgaaggg tcttcttatg tgttccagcc
    10321 ttccggcgaa ataggggtcg tcgaagatcg cctcgatgag gcccgcaccc tgcgttctga
    10381 gatttgctat gttgtggcga agactgtccg gatggctgaa ggtgacggct gtcggtttcg
    10441 agaggaaaca gcccttcctg tcgaagaaca ccttggaaaa gaagaccagg tcctcgccgt
    10501 tgttcagatc cgtggcaaac tcgaactttc tcgcgtggct tcccctcacg aggacggcgg
    10561 atgtcgtgta gggtatccgt ttgaggagaa gtgcctggca caactccctg gaaacgatgt
    10621 cacgcggtcc cctgtagggc cggaaggata ccctgccggc atcgtccacc gatgaggcgg
    10681 tcgtcgttac gaaatctatg tcggggttct cgagcatgac ggtgacccgt gtttccacag
    10741 agtcgtcggt gaggtaatcg tctgcgtcca gaaaggcgat gaaatctccc tttgccatcc
    10801 tgagcccctt gtttctggcg gcagaaaccc cctggttttc ctgataatga tagacaaccc
    10861 tctgatcagc gattttcctt actatttctc ccgttgcgtc cgtcgaaccg tcatcgatga
    10921 ctatgacctc gaggttgccg gaggattgtc tcaggcacga tcctatggcc ctgtgcacat
    10981 atgtcccata gttgtacgcg ggcacgatga tggatacgag tggctgctcc ttcaagaccg
    11041 tctaccgcct tcccttctct tcgaatgccc ccaggagcac tgcctccatc tcgtcggcgt
    11101 tgcgttccat ggtgaaggac tccatcttct tccgcgcgtt ccttcccatg acttcctgtc
    11161 tcaccgggtc tgttagggcg cagatggcgg cgctcagagc ctcgatgttg tctgcctcgt
    11221 cgatgacgaa gccctcgtcg ggagagacga aggcgcatgc cccgttccac cgggtggtga
    11281 tgatgggaag gcccgatgcc atgccctcca tcgtggtcag cgagcaggcg tcgtagtagg
    11341 tggggtgtgc gagcacgtgg gcctcacggt agacctcttc cggcgcggag cgctcccccg
    11401 tgaaggtgac gcggtccgcg atgcccgccg cgtcgatgag gccccggtac ctctccttct
    11461 tcccccggcc catcacggtg agccgcacgt cctttccctt ttccaccgcc cttcccatgg
    11521 ctgcgatgag gggggagagt cccttgagcc tgaagttccc cgcgcagaag aggatcgtcg
    11581 tggggcctgc cggccgctcc ggagccggct taaagcggtc cgtgtcgaca ccgttgtaga
    11641 cgacgtcgaa ggtgttcctg tcaatgccgt agtgcgccga catgtggtcc cggaccatgt
    11701 cggatatggc cactatcctc gtgaacctgt gttttttcac ggagtatccc tctatccagc
    11761 gctggatggc ctggttgagg ctgttcctca gaaggaacgc tttcatgccg gcgcctcccg
    11821 gcccatagct cacgatctcc ctttccatcc agacgctctg cacccctcca tggctctggt
    11881 aaacgtcgag gtcgagaatg ttgccgtagc ccagcatgat gtcgggacgg atcctgtcga
    11941 gggcctgtct gtgcgtcagg gcgaaggaca ggttccggcg ccaccggggg aaggaattcg
    12001 tgcgaaggcg tatgagcctg acaccttccg tctcgacgcc gcggccgcag aagacacaga
    12061 tctcgtgacc tttgccggca agtctccggg caaggtcaaa ggtgtagcgt tcggcgccgc
    12121 ctttcttggg gtcgaaattg tatatagcga gaccgatctt catcctcagt cactccgcgt
    12181 cgatgagggg tccggcgagg gaggtcacgc gcgcctgcca ctggtgagga ggaaatcgta
    12241 gaattgcttc atggcctttg cctggaggcg aaagctccac tcgctgtcgg cgcggttccg
    12301 ggcgttctcc ccgaaggcgc gccgtctggc ggcgtcgctg acgagaagct ccatcttgcc
    12361 ggcgaggtcc gccgcgtccc aggtcgtcac gtacccggtc ctgccgtcct ccacgaggtc
    12421 cgggagcatt cccctgtctg acacgatggc gggccgcccc atgctgagca cctccctgag
    12481 cgcccggccg ctgccgtcgc ttcccgcgcg catcatgatg taaagatcga aggtgtgtat
    12541 catcgagaag tagtcgtcga tgcggtatcc ggcgatcacg acgtctttct cgatgccgag
    12601 ttccttgagt ggtttcatga cgctcgtttc tatttgggaa ctgcggccca cgatgacgag
    12661 cttgaccctg tccacacgtg acctcagcat gccgaaagcg ctgaggatga catcgtagcc
    12721 gcggtcgggc ttgagtctcc cgatgacgcc gatgacccgt tcatcgggag cgatcccgag
    12781 ttcggcgcgc aggtccttta agggcccttc gtaaggcttg acccccggcg ggatgacgcg
    12841 aaggcgctct ccgggatagg cgaagctctc cctgtcgcgc gcggcgagcc tttcgctgta
    12901 ggttaccagc ccgtccgttc ttgccatggc ccaggccatg aagatgttcg cggggagccc
    12961 gtcccgcttg tggtccgtcc tcacgaggag gggccgtgcc ctggagaagg aaagcgccgt
    13021 gacggcggtg tagtaatcat gggaaagatg ggtatggacg atgtcgaatt tctccttcaa
    13081 cacgtacgga ttgaaggaga tgaggtcatg ccaccagtcg cgggcggaga aatacctgtt
    13141 gagcctcaaa ccctcatagc agcgaagccc tcttttgcct gcctccctgt cgacggtcct
    13201 ttcgcgaaaa tccgcgggcg acttcctgca ggcgaggacg acgtcgacgc cctcgttccc
    13261 gaggttctcg cagagggaga gcaccggttc ggcgggcccc gtccatttcc agtcgctgaa
    13321 gaggtgaagt accttcatct tccgtagaat tcaccgatcg tccggcatac gaaaccgatc
    13381 tcttcttccc gaagcgaggg gtggacgggc agggagatga tctgccgtga cgccttttcg
    13441 gctttcggga aggagccctc gccatacccg aggtaactgt agaccttttg aaggtggatg
    13501 ggtgtcggat agtggacgag ggtggtcacg cccttttcgc tcaggtatgc cctcaatgcc
    13561 tccctctctt caacggcgat gacgtagagg tgatagacgt ggaaggcttg aggtaccgtg
    13621 acgggccggc gaacgggcag gccttcgagg cccctgtcgt acagcgtggc gttgcgcctg
    13681 cgcccttcgt tccagccgtc gagatggggg agcttgacgg aaagcagggc ggcctgcagt
    13741 tcgtcgagac gggagacaaa accctgaatg tcgtgaacgt gcctgtccga ctggccgtgc
    13801 gcgcggagca tgagcattct tctgaagacg tcttccctgt cggtgacgac gatcccgccg
    13861 tcgccatagc agccgaggtt cttcgtggga tagaaactga aggcggccac gtcgctcatg
    13921 gttcccgcgt tccggtcctt gtaccgggag ccgtgtgcct ggcaggcgtc ttccatgacc
    13981 ctgaccccgt gcctttcgca gatatcccgt atctcgtcca tcctgcacgg ctgtccgtaa
    14041 agatggacgg gcacgcagag ttttatgtcc ttctccttgc cgagaagctc ttcgagcgcg
    14101 gcggggtcca tgttgtaggt atccgcctct atgtcgcaga ggcgagggac caggcctccc
    14161 tcggaaaggg ccatcgccgt ggcgatgtac gtgttgggcg tggtgacgaa cttgtcccct
    14221 tccgtgagac cgagggcaag cccccctatc tttatggcat cggttccgct cgcgacgcca
    14281 acggcgtacc tcgtgcccac gcaggcggcg aaggcctctt cgaaggactt gacctccctg
    14341 ccgaggatgt attccccgga ggagagcacc ttttcgaatg ttctcacaag ttccgccttg
    14401 atcccggcgt ggtctttttc tatatcgaat attctcacgt tcacgatgcg gtaccgtcct
    14461 tttcggtcat ggcacgcccg acggcttacc agtattccat cctgtgcttc cggtagaaat
    14521 cgatggtcct cgagatcccc tcatcgaggg acgtcgccgg ttgccagccc gtcgtcccgg
    14581 tgatgcgcgt tatgtcggcg taataatcac cgggttccac ctcctt
//
LOCUS       DAOQTR010000003        13393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_5205_length_13393_cov_3.898111, whole genome shotgun sequence.
ACCESSION   DAOQTR010000003 DAOQTR010000000
VERSION     DAOQTR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 13393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13393
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5205_length_13393_cov_3.898111"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..311)
                     /locus_tag="PKN85_00170"
     CDS             complement(27..311)
                     /locus_tag="PKN85_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT42845.1"
                     /translation="MLEVTSLQKSFDGFKAVNNASLSVKEGEVVAVIGPNGAGKTTLF
                     NLITGVLKPDGGKVLFKGEDITGLPSYKVCRRGMSRSFQVVNVFPRRQTL"
     gene            complement(334..1272)
                     /locus_tag="PKN85_00175"
     CDS             complement(334..1272)
                     /locus_tag="PKN85_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT42846.1"
                     /translation="MKNPLLRGKLPWVIAALIVLFLLPVFLDRFYVYLAAIILLTGLS
                     ATSLNFVLGYGGVFQFHHAVFYGIGAYGTALMIIKSNLSPWLGFIVGPVVAGILGFVI
                     GVICIRLSKLYFGMLQISLGSLVWAIVYRWYSFTGGDDGIHGVPLPDIISSPNGAYYF
                     TLIVTAVSMFILYRMIRSPFGSALQGIRDNPVRSEMIGVNVKLQQLLALTIAGFFGGV
                     AGSLFVVVDNTVFPDMMFWTLSLELIIMCLLGGWFSFLGPMVGAGIIVLLRTYVSGLT
                     VYWALILGIIMMLVIFFLPNGVLGYLDRFVKKKARS"
     gene            complement(1283..2182)
                     /locus_tag="PKN85_00180"
     CDS             complement(1283..2182)
                     /locus_tag="PKN85_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT42847.1"
                     /translation="MESVLGFISPKILSQILVGLSRTVILFIVSSGLSLILGVLRIPN
                     VAHGSLYMIGAFMAYTVSHLFGGGNMGFWMALVLAPLGVAVVSFAAERSLFQFLYERE
                     HLMLLLLTFAMSLVFGDLVKMVWGGEYRSVPVPSIFQGYINLFGGLPFPIYSFFLLVV
                     GPVIAILLWLLTNKTKIGKISRAAAVDREMVGAVGINVSWVFATVFIIGCLLAGLGGV
                     LVAPTVSVTLGMDHTLIMEAFLIVIMGGLGNVWGALLGSLIFGLTQSLGILVWPQFGI
                     VFPYLAVVVVLLFRPTGLLRSTW"
     gene            complement(2207..3424)
                     /locus_tag="PKN85_00185"
     CDS             complement(2207..3424)
                     /locus_tag="PKN85_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT42848.1"
                     /translation="MKRYSFLALASVLLLSVLCVYSVSQAANTIKVGIVDTYTGPATT
                     FTNDVLDGFKLAVQKINASGGILGRKIEYTTRDEKFKPDIGLAMAKELVLKEKVDILM
                     GTINSATTLAISDFVRKEKIPFFVTFAKSDKIVAEKGHRYIFNMNENTEMAGRAAAIA
                     LAKKPYVKYWIAGDDYEYGHAIAEGVWDNLKKLKPSVQKVGETWWKVGEADFTPYITQ
                     ILAAKPDFIIVATGGSGMVNFQKAAKATGLSQKVPFYQHTATELSVLAPQGQNAPEGV
                     YGTANYFFYYPDTAQNKAFVADFKKAYNRNPRIGALYGYMTAQFIAEGYKKAGKIDKE
                     KFVTALEGMSLDSPVGKLEIRKCDHQLLLPMYFGVTKKDAKYEFLTASGIETIQGKDY
                     VPSCEEVLKHRKK"
     gene            complement(3523..4206)
                     /locus_tag="PKN85_00190"
     CDS             complement(3523..4206)
                     /locus_tag="PKN85_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT42849.1"
                     /translation="MRKLVIKKDKTIRQKVYHHIREEILKGAIAPKERLIEAKIAGEI
                     GTSRTPVREALHNLELEKLVVSIPRVGYVVRGMDMEEVEQICEIRAAIESLAIAWATA
                     KQRDRLIGALKRNIENQKKQISKGNLNGYVELDAQFHDIIARLAGSDRLLELTQTLRR
                     HMLRYRIQCIYMTETAERSMHGHERIVTAIEGGDSQEIAAAVKTHLIQARDDILYYVF
                     RDPDGDASR"
     gene            4259..5473
                     /locus_tag="PKN85_00195"
     CDS             4259..5473
                     /locus_tag="PKN85_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNT42850.1"
                     /translation="MKISLPHGLWNGERTLEADFPARWDVDVLSMEGDRARPIGGAEY
                     RAAIMPLVPLMKGAKEVCVLFDDLSRPTRTYEIVPTLLEALAGADIDDSRVRFLCALG
                     THSPHNNADFRKKLGNEVLQRFPVYNHNCYENCETLGRTALGTPVTINKEYLTCDLRI
                     GMGSFIPHQFCGFGGGYKMVFPGIAHIDAIQYHHGLLLQQNLESCWGLGNHSCNAILS
                     DMREAGRMARLDIKIDVFVNSSSRATRVFAGHPDSLYPAMIEGALAHYATNAAGKYDI
                     VFANTFGKANEAVIAASTSEEILTPDGGCLVILNDIEEGQVIHYLMGRFGKDLWGRLG
                     RGERPLHEKVKRLFLYSRHKDHAGSYWFGKKDDITWVDDLPALIGELDREYAGRPVRV
                     AVIPDGTVQMIG"
     gene            5629..6114
                     /gene="ybaK"
                     /locus_tag="PKN85_00200"
     CDS             5629..6114
                     /gene="ybaK"
                     /locus_tag="PKN85_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNT42851.1"
                     /translation="MSKDKTYVTPAVRFLRERKVEFSEHPYQYVEHGGTETFAKQYHV
                     DEHMVVKTLIMEDEDKRPLVVLMHGDREVSTKELARVMGVKKVGPCTPETAQKHSGYM
                     VGGTSPFGTRKAMPVYMEETILALPKIYINGGRRGYLIAMGPHDIVKTLQPKMVNVAI
                     E"
     gene            6329..7039
                     /locus_tag="PKN85_00205"
     CDS             6329..7039
                     /locus_tag="PKN85_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT42852.1"
                     /translation="MSPDLSALLIAAVSIGFFHTLFGPDHYVPFVMLSWSRKWSTLKT
                     ALVTFACGLGHILSSIVLGFVGVALGVALEKLELIESTRGTVAAWLLIAFGLAYTVWG
                     IRRAVLNKPHRHVHVHGTSDVHEHEHTHHGSHAHFHEDEGRAVTPWILFIIFVFGPCE
                     PLIPLLIYPAARKSFMGVALVSLAFGVVTIATMMAVVLLMRAGLSFKVLDRAERYTHA
                     IAGLTILTCGLMIQFLGL"
     gene            7047..7508
                     /locus_tag="PKN85_00210"
     CDS             7047..7508
                     /locus_tag="PKN85_00210"
                     /inference="COORDINATES: protein motif:HMM:NF008675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT42853.1"
                     /translation="MKKPGNDEFLHVINEMFSEQIPFNTLLGLSIESMSPGGVTVSFR
                     MRDELMGHRKRRMVHGGVISSVIDVTGGLVAFMGAQERMDETLGAKLERFGRVSTIDL
                     RVDFLRPGTGTAFTAIARTLRTGNKVAVTRTELTNDTGELIAVGTGSYTVS"
     gene            7759..8589
                     /locus_tag="PKN85_00215"
     CDS             7759..8589
                     /locus_tag="PKN85_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT42854.1"
                     /translation="MAHFGPHFGEEPPISGTRGSGNIFFSFCNLRCVFCQNYQISHEG
                     RGNTVTIDELVEMFFSLEAMGCHNVNLVSPTPYVPFIAAAIGEAKRRGIGIPFVYNTN
                     GYDSVDALRALAGLVDIYLPDLKYWSPAIAQKLSGAAAHAPYPEFARAALVEMKRQVG
                     HLVVEDGIAKKGLLVRHLVLPGGLAGSRPALGWIAENVGKRTWVGLMSQYLPLHRSGE
                     HPVLSRRIRQEEYDDLVSFLTTNGMDNVFTQDLESAPLYVPDFGKEQPFGEGEGEARK
                     "
     gene            8891..9373
                     /locus_tag="PKN85_00220"
     CDS             8891..9373
                     /locus_tag="PKN85_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HNT42855.1"
                     /translation="MELKTVKIDIPEGLNIVIGQSHFIKTVEDLYELLVGSSPSLKFG
                     VAFSEASGPCLVRYEGNDEALAKLAADTSFTLSCGHTFVIFIKDGFPINVLNAVKGCQ
                     EVCRIFCATANPLEVVVAETTGGRGIMGVIDGARPAGIEKDEDKRARKEMLRKFGYKL
                     "
     gene            9663..10820
                     /locus_tag="PKN85_00225"
     CDS             9663..10820
                     /locus_tag="PKN85_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT42856.1"
                     /translation="MPPYVFGIVRDLLIEARRKGEDIIDLGMGNPDLPTPKHVVSKLV
                     EAARNPRNHRYSATRGITNLRLAIANWYRRRYGVDIDPEDEVVVTMGAKEGIGHLVLA
                     TISQGEVVFVPNPAYPIHSYSVVIAGGDLRTIPLLPREEFFERLNIAVKTTWPLPKML
                     IISFPNNPTTEVVEKDFFERTVAFAKEHDMMVVHDLAYADLAFDGYRAPSFLEVPGAK
                     DVGVEFFSMSKSYSMPGWRVGFAAGNKRMINALSRIKSYFDYGVFQPIQIASIIALNE
                     GDADVPEIVEKYRARRDVLCEGLCRLGWEVEKPRATMFVWAKIPEKYAAMGSIEFSKF
                     LLTEAKVATSPGIGFGEFGEGYVRFALVENEHRIKQAVKGIRNLLRPTGRS"
     gene            10817..12103
                     /locus_tag="PKN85_00230"
     CDS             10817..12103
                     /locus_tag="PKN85_00230"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNT42857.1"
                     /translation="MIGIGIIGLGTVGTGTYRILTEHAALIRRRTGLDLRVVRVAEVD
                     ERKLRGKGLARNVVTSDAMELIHDERVGIVVELIGGMRPASDFIGAALGLGKQVVTAN
                     KALLAEKGDELFRLAAKKGCEIGFEASVCGGIPVIRAIRDGLVGNDFSYILGILNGTS
                     NYILSRMTDEGISFAEALKDAQRKGYAEKDPTFDVEGIDAAHKLSLLVRLAFASPVTM
                     DRITTRGIAKIDPVDIGFARDFGYKIKLLAIAKKRGAGIEARVEPAMLPVNHPMSSVN
                     GVYNAVYIVGDMTGPNLFYGKGAGSDPTGSAVVSDIVDIAKRISGAGGTGVRPLVAAG
                     RFKEGRASSVPYYLRVMAKDMPGVLSKISGILSKYRISISAVTQQGRKLSGYVPVVMV
                     THDAPEEGLMKAKKEIDALSLTKGESVHIRIEEGNL"
     gene            12144..13331
                     /locus_tag="PKN85_00235"
     CDS             12144..13331
                     /locus_tag="PKN85_00235"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HNT42858.1"
                     /translation="MKYIVLIGDGMADFPLEELGGKTPLMAANKPNMNAAARKGLTGK
                     VKTVPDGFPPGSDVAGMSIFGYDPALYYTGRAPIEAFGMGIPMGSDDVAYRCNLVHIG
                     TGNGGDTMGDYSGGHISTEEARILVASLNDELGSEGITFFPGVGYRHIMIWKRGAWEL
                     STTPPHDITGKQIAGYLPEGDGAAMVRSLMDRSRRVLGSHDINRKRIDAGKLPANGIW
                     LWGQGKRAGLPPFREKFGLTGATVCAVDLVRGISSLVGFTTPVVEGATGYLDTDYGAK
                     ARAALKLLEDHDIVYIHVEAPDEASHNGSIPEKIKAIERIDRDVLGVLLKETDRDTRF
                     LIVTDHATPIAMRTHYATPVPFAVYDRSDPASEEGGDYDENAGGAVISGEEMIRILIG
                     GRQ"
BASE COUNT         2864 a         3917 c         4038 g         2574 t
ORIGIN      
        1 ggtgaggaca taaccgggct tccctcctac aaggtctgcc gccggggaaa gacgttcacc
       61 acctggaagg accgcgacat accccggcgg cagaccttgt aggagggaag cccggttatg
      121 tcctcaccct tgaagaggac ctttccaccg tccggcttca atacgcccgt gatcaggttg
      181 aaaagggtcg tcttgccggc tccgttcggc cctatgacgg cgacgacctc gccttccttc
      241 acggagagac tggcattgtt gacggccttg aagccgtcga aggatttctg aagtgacgtc
      301 acttccagca cgtaaggctc cttgcgcgca tcatcagctt ctcgctttct tcttaacgaa
      361 cctgtcgagg tatccgagga cgccgttggg caggaagaag atgacgagca tcatgatgat
      421 gccgaggatg agggcccagt acacggtgag gccgctgaca taggtcctca ggaggactat
      481 gatgccggct cccaccatgg ggccgagaaa cgagaaccac ccgccgagga ggcacatgat
      541 gataagctcc agggagagcg tccagaacat catgtcggga aagaccgtgt tgtccaccac
      601 gacgaagagc gaccccgcga cacctccgaa gaaaccggcg atggtcagcg ccaggagctg
      661 ctgaagcttc acgttgacgc cgatcatctc ggagcgcacc gggttgtccc gtatcccctg
      721 gagagcgctc ccaaaggggg accgtatcat cctgtagagg atgaacatgg acacggcggt
      781 gacgatcaaa gtgaagtagt atgcgccgtt gggcgaggag atgatgtcgg gaaggggtac
      841 gccgtggatg ccgtcgtcac ccccggtgaa ggaataccac cggtagacaa tggcccacac
      901 gagggacccc agcgatatct ggagcatccc gaaatagagc ttggaaagac gtatgcagat
      961 gacgccgata acgaacccca ggatgccggc aactacgggc ccgacaatga acccgagcca
     1021 gggagacagg ttcgacttga tgatcatgag cgccgtaccg tacgcgccga tgccatagaa
     1081 gacggcatgg tggaactgga agaccccgcc gtaaccgagg acgaaattga ggctcgtcgc
     1141 gctgaggccc gtaaggagaa tgatcgccgc caggtacaca tagaacctgt ccagaaagac
     1201 gggaaggagg aaaaggacga tgagcgcggc gatgacccag ggaagctttc cgcgaaggag
     1261 agggttcttc atggacggcc cctcaccatg tcgacctcaa gagccccgtg ggccggaaaa
     1321 ggagcacgac gaccacggcc aggtaaggga agacgatgcc gaactgaggc cacacgagaa
     1381 tacccaggga ctgggtaagg ccaaatatga gcgagccgag aagggcgccc cagacgtttc
     1441 cgaggccccc cattatgacg atgaggaagg cctccatgat aagggtatgg tccattccta
     1501 gggtaacgct caccgtgggg gcgacgagga cgccgccaag accggccaga agacagccga
     1561 ttatgaaaac ggttgcgaag acccagctca cgttgatgcc cacggccccc accatctccc
     1621 tgtccacggc ggccgcgcgc gatattttgc cgatcttcgt cttgttcgtg agaagccaca
     1681 gcagtatggc aatgacgggc cccacgacga gaaggaagaa gctgtagatg ggaaagggga
     1741 gcccgccgaa aaggtttatg tagccctgaa agatggaagg cacggggacc gaacggtatt
     1801 cgccccccca caccatcttg acaaggtctc cgaaaacaag ggacatggcg aaggtgagca
     1861 ggaggagcat caggtgttcg cgctcataga ggaactgaaa gagggacctc tcggcggcga
     1921 aactgaccac cgccaccccc aggggtgcca gcaccagggc catccagaaa cccatgttgc
     1981 cccctccgaa gaggtgcgac accgtatagg ccatgaaggc gccgatcatg tagagggaac
     2041 cgtgcgccac gttggggatc ctgagaaccc ccaggatcaa ggacaggccc gacgagacaa
     2101 tgaagaggat gaccgtgcgg gacaggccca cgagtatctg gcttaagatc ttcgggctta
     2161 tgaagccaag gacgctctcc attcttctcc cttgtcttcc ttcttcctat ttcttgcggt
     2221 gtttcagaac ctcctcgcag gacgggacgt agtccttgcc ctggatcgtc tcgatgccgc
     2281 tggcggtcag gaactcatac ttcgcgtcct ttttcgtgac gccgaagtac atggggagca
     2341 ggagctgatg gtcgcacttg cggatctcaa gcttgccgac agggctgtcg aggctcatgc
     2401 cctcgagggc cgtgacgaat ttctccttgt cgatcttgcc ggccttcttg tacccctcgg
     2461 cgatgaactg cgccgtcatg taaccgtaga gggcaccgat ccggggattg cggttgtatg
     2521 ccttcttgaa atcggccacg aaggccttgt tctgggcggt gtcgggatag tagaagaagt
     2581 agttggccgt tccgtagacc ccttcggggg cgttctgtcc ctggggggcg agaacggaaa
     2641 gctccgttgc cgtgtgctgg tagaagggga ccttctgcga aagacccgtg gccttggccg
     2701 ccttctggaa gttgaccatg ccggagccac ccgtggcgac gattatgaag tccggtttcg
     2761 cggcgagtat ctgcgtgatg tagggggtga agtccgcctc gcccaccttc caccacgtct
     2821 ctcccacctt ctggacggaa ggtttaagct ttttgaggtt gtcccagacg ccctcggcga
     2881 tggcgtggcc gtactcgtag tcgtcgccgg ctatccagta cttgacgtag ggcttcttcg
     2941 cgagcgcgat ggcggccgcc ctgcctgcca tctcggtgtt ctcgttcatg ttgaagatgt
     3001 agcggtgtcc cttctcggcg acgatcttgt cgctctttgc gaaggtgacg aagaagggta
     3061 tcttctcctt cctcacgaag tcggagatgg ccagcgtggt ggcgctgttg atggtgccca
     3121 tcaggatatc gaccttctcc ttgaggacaa gttccttggc catggcaaga ccgatgtcgg
     3181 gcttgaactt ctcatccctc gtcgtgtatt cgatcttcct gcccaaaatg cctccggagg
     3241 cgtttatctt ctgcacggcg agcttgaagc catcgagaac gtcgttggtg aaggtcgtcg
     3301 cgggccccgt gtaggtgtcg acgatgccca cttttatggt gttcgccgcc tgggagacgg
     3361 agtacacaca caggacggac agaagcaaca ccgacgcgag cgcaaggaag gaatacctct
     3421 tcataccagc acctccgttt ggtttttatg cgttattctg aggtttgcac agaaacccct
     3481 ttttgtcaat caaaacgtcc tctgtggggt ccgctgctgg ggctatcggc tggcgtcgcc
     3541 gtcgggatcg cgaaagacat agtaaaggat gtcgtccctc gcctggataa ggtgtgtctt
     3601 caccgcggcg gcgatctcct gagagtcccc cccttcgatg gcggtcacga tccgttcgtg
     3661 gccgtgcatc gatctctccg ccgtctcggt catgtagatg cactggatcc ggtacctcag
     3721 catatgcctg cgcagggtct gggtgagctc cagcaggcgg tcgctgccgg caaggcgcgc
     3781 aatgatgtcg tggaactgcg cgtccagctc aacgtagccg ttgagattgc ctttcgatat
     3841 ctgtttcttc tggttctcga tgttcctctt gagggccccg atgagcctgt ccctttgctt
     3901 cgccgtggcc cacgcgatgg caagactttc gatcgccgcc ctgatctcgc atatctgctc
     3961 cacctcttcc atgtccatgc ccctgaccac gtagcccacc cgggggatgg agacaacgag
     4021 cttctccagt tcgaggttgt ggagcgcctc gcgcacggga gtccgggatg tgcctatctc
     4081 cccggcgatc ttcgcttcta taagcctctc cttgggggcg atggcacctt tgaggatctc
     4141 ctcgcgtatg tgatgataga ccttctgccg tatcgtcttg tcttttttta ttaccagttt
     4201 tctcattaat ttcgtatact gtaccctagt tgttgttatc attacaagtt ctaaatttat
     4261 gaaaatatcc ctgccccacg gcctctggaa cggtgagcgc accctcgagg cggacttccc
     4321 ggcccgttgg gacgtggacg tgctgtcaat ggaaggtgac agggcacggc ccatcggcgg
     4381 agcggaatac cgggccgcca tcatgcccct cgtcccgctc atgaaaggcg cgaaggaggt
     4441 ctgcgtcctc ttcgacgacc tctcccggcc gacacgcacc tacgagatag tcccgacgct
     4501 cctcgaggcc ctggcaggcg cggacatcga cgactcccgg gtgcggttcc tctgcgccct
     4561 gggcacccac tcccctcaca ataacgccga tttcagaaag aagctcggca acgaggtgct
     4621 tcagcgattt cccgtttaca accacaactg ctacgagaat tgcgaaacgc tgggaaggac
     4681 ggccctggga acacccgtta ccattaataa agaatacctg acctgcgacc tcaggatagg
     4741 catgggctcc ttcatccccc accagttctg cggtttcgga ggcggataca agatggtctt
     4801 tcccggcata gcccacatcg atgccattca gtatcaccac ggtctccttc tccagcagaa
     4861 cctggagagc tgctggggcc tcggcaacca ttcctgcaac gccatcctgt cggacatgcg
     4921 cgaggcggga aggatggctc ggctcgacat caagatcgat gtcttcgtca acagctcgtc
     4981 ccgcgccaca cgcgtcttcg ccggtcatcc cgactccctc tatcccgcga tgatagaagg
     5041 ggcgctcgcg cactacgcga cgaatgcggc cggaaagtac gatatcgtct tcgccaacac
     5101 cttcggcaag gccaacgagg ccgtcatcgc cgcctccacg tcggaggaga tccttacccc
     5161 cgacggcggc tgtcttgtca tcctgaacga catcgaggaa gggcaggtca tccactacct
     5221 catgggaagg ttcggaaagg acctctgggg aaggctcgga aggggcgaaa gaccgctcca
     5281 tgaaaaggtg aagaggctct tcctctactc ccgccacaag gaccacgccg gctcctactg
     5341 gttcgggaaa aaggatgaca tcacctgggt cgacgacctt ccggccctca taggcgaact
     5401 cgacagggag tatgcgggca gacccgtccg ggtggcggtg ataccggacg ggacggtgca
     5461 gatgatagga tgaagacggt gttacgtttt aggttttaag ttttaagcag aagacaagag
     5521 aagaaagggt ggtctgtgcc gtcttttgcg cgagatcgtg atccggggtt ctttacggcc
     5581 ctgcttttaa ctaaaaactt aacacttaag acttaaaacg gtatctttat gtccaaggat
     5641 aagacctatg tgacgcctgc cgtccggttc cttcgggaga ggaaggtgga gttctcggag
     5701 cacccctatc agtacgtgga gcacgggggg acggaaacgt tcgcgaagca gtaccacgtc
     5761 gacgagcaca tggtggtgaa gaccctcatc atggaagacg aggacaaaag acctctcgtc
     5821 gtcctcatgc acggcgacag ggaggtctcc acgaaggagc tcgcccgcgt catgggcgtc
     5881 aagaaggtcg ggccctgcac gcctgagacc gcgcagaaac actcgggcta catggtcggc
     5941 ggcacatcgc ccttcggcac ccgcaaggcc atgcccgtct acatggaaga gaccatcctc
     6001 gccctgccga agatctacat caacggcggc aggcgcggct acctcatagc catgggcccc
     6061 cacgacatag tgaaaacgct gcagccgaag atggtaaacg tggcgataga gtaaggacgg
     6121 ttcaagggtt caagggttta agggttcaga agaaagacgg tcaaggaagg gcggttgtca
     6181 acacgatgtg gacaatcggg tgcaaagtta ttataatgac acgaaacttc aacaggtgcc
     6241 atgcgtccca tacgtcccat acgtcctatc cctcctatag gtcctgtcgg tctcgcagcg
     6301 tcctttaaga ccacacccaa ggccccctat gtcccctgac ctgtccgccc tcctcatcgc
     6361 tgccgtgtcc atagggttct tccacaccct cttcggcccc gaccactatg tacccttcgt
     6421 gatgctgtcc tggtcgagga aatggtcgac gctgaagacc gcattggtga ccttcgcctg
     6481 cgggctcggg catatcctga gctccatcgt gctgggtttc gtcggggttg cgctcggtgt
     6541 agcgctcgag aagctcgagc tcatagaatc gacgcggggg acggtcgcgg cgtggctcct
     6601 tatcgccttc ggccttgcct acaccgtgtg gggcatacgc agggcggtcc tcaacaaacc
     6661 ccacaggcac gtgcatgtcc acggcacctc agacgtccac gagcacgagc acacccatca
     6721 cggcagccat gcccatttcc atgaggacga aggaagggcc gtcacaccct ggatcctctt
     6781 cataatattc gtcttcggcc cctgcgaacc cctcataccc cttctcatct atcccgccgc
     6841 ccgtaagagc tttatggggg ttgccctcgt gagcctcgcc ttcggtgtcg taacgatagc
     6901 cacgatgatg gccgtggtac tcctcatgag ggcgggttta agcttcaagg tccttgacag
     6961 ggccgaacgt tacacccacg ccatcgcggg cttgacgatc ctcacctgcg gcctgatgat
     7021 ccaattcctc gggctctgag ggaacgatga aaaaacccgg caacgacgaa ttcctccatg
     7081 tgatcaacga aatgttcagc gaacagatcc ctttcaacac gttgctcggg ctcagcatag
     7141 aatcgatgag ccccggaggc gtgacggtgt cctttcggat gcgcgacgag ctcatgggac
     7201 accgaaagcg ccgtatggtc catggcggcg tgatatcctc cgtcatcgac gtcacgggag
     7261 gactcgtcgc cttcatgggc gcacaggaaa gaatggacga aacgctgggg gccaagcttg
     7321 agaggttcgg gcgggttagc accatcgatc tgcgggtcga cttcctccgc cccggcacgg
     7381 gcacagcctt caccgccatc gcacgaaccc tgagaactgg gaacaaggtc gccgttacga
     7441 gaacggaact gaccaacgac acgggagagc tcatcgcggt gggaacagga tcatatacgg
     7501 tctcctgaag accaggtaat gggttccggg tcatgggtca tgggggaaaa agcaaagaac
     7561 agtggaaatc agggtttccc cggttttcct atcacccata acctataacc cattacctgg
     7621 tcttccgttt ctgctatact attgccgatg aaacccgacc tttccatgct tgcaccctgc
     7681 actgtctgcc cccgtcggtg cggggttgac cgccttgccg gggaaagggg gttctgcggg
     7741 gcaggcgggg atattgcggt ggcccatttc gggccccatt ttggggagga gccgcccatc
     7801 tcggggacga gggggtcggg gaacatattc ttttccttct gcaacctgcg gtgcgtcttc
     7861 tgtcagaact accagataag ccatgagggt cggggaaaca cggtgaccat tgatgaactt
     7921 gtggagatgt tcttctccct cgaggcgatg gggtgccaca atgtcaatct cgttagtccg
     7981 acgccatacg tcccctttat cgccgcggcc atcggagaag cgaagagaag aggtataggc
     8041 atacctttcg tgtacaacac gaacggctac gacagtgtcg acgcccttcg cgccctggca
     8101 ggcctcgtcg atatttacct gcccgacctg aagtactgga gtccagccat cgcgcagaag
     8161 ctgtccggtg cagcagccca cgcaccttac cccgagttcg cccgcgcggc ccttgtcgag
     8221 atgaagcgcc aggtggggca ccttgtcgtc gaggacggca tcgcgaaaaa gggcctcctc
     8281 gtccgccatc tcgtgctccc cggtggcctt gccggttccc gtcctgcact cggctggatc
     8341 gccgaaaatg tgggcaaacg gacctgggtg ggcctcatgt cccagtacct tcccctccac
     8401 cgctccggcg agcacccggt gctctcccgc aggatacgtc aggaagaata cgacgacctc
     8461 gtttccttcc ttacaactaa tggtatggac aacgtcttca cccaggacct cgaaagcgcc
     8521 cccctctacg tccccgactt cggcaaggaa cagcccttcg gcgaagggga gggagaagca
     8581 cgtaaatgac agaaaaaacg aaaagatcgt ctcacgcccg cttcgctcga gttcacagag
     8641 ttcacaaaga gagaagagaa gaggaaagac tgttccctga tcccggagac taccgggatc
     8701 agggaacatc ttcgattccg cccttccctt atgacgagcg gaatggaaaa gagttgtctt
     8761 tgcctcccgc cgggacggcg gcaatccttt ctctcttttc cgtctctctt tgtgaactct
     8821 gtgaactcga gcgaagcggg cgggcgtgag ataacgtttt taatcttttt taccaactaa
     8881 aggaggcaca atggaactca agaccgtaaa gatcgacata cccgaaggcc tgaacatcgt
     8941 catcggccag tcgcacttca tcaagaccgt cgaggacctc tacgagttac tcgtcgggtc
     9001 ctctccgtcc ctcaagttcg gcgtcgcctt ctccgaggcg agcgggccct gcctcgtgag
     9061 atacgagggc aacgacgagg ccctggcgaa gctcgccgcc gacacgtcct tcaccctctc
     9121 ctgcgggcac accttcgtca tcttcatcaa ggacggtttt cccatcaacg tcctcaatgc
     9181 cgtgaaggga tgccaggagg tctgccggat cttctgcgcc accgcgaacc ccctcgaggt
     9241 cgtggtggcc gaaacaaccg gcgggcgggg catcatgggt gtcatagacg gcgcgagacc
     9301 ggcaggcata gagaaggacg aggacaagag ggcccggaag gagatgctca ggaagttcgg
     9361 ttacaagctc tagaagagct gttgcgggtt gcgggttgcg ggttgcgggt tgcgggttgc
     9421 gggttgcggg ttgcgggaaa aaagaccgtg aaccaggacg ggctgtcaac acgattaccc
     9481 agaagtgcat gctgactagt gcaacagagg atgttgtctt cggcccttga ctcgcaactc
     9541 ggaacccgga acgcgcaacg atcgttttat ggaacaattc ttgcttggct tttgtgttga
     9601 ttgtgtcccc ggggatgtgg taattttcaa cataaatgga agaattttac aggatatccc
     9661 ggttgccgcc ctacgtgttc ggcatcgtcc gggacctttt gatagaggca cggagaaaag
     9721 gcgaagacat catcgacctc ggcatgggga accctgacct gccgaccccg aagcatgtcg
     9781 tttcgaagct cgtcgaggcg gcgcggaacc ccaggaacca ccgctacagc gcgacacggg
     9841 gcataacgaa cctccgcctt gccatcgcca actggtacag gaggcgctat ggggtggaca
     9901 tcgaccccga ggacgaggtg gtcgtcacga tgggcgccaa ggagggaatc ggccatctcg
     9961 tactggctac catcagtcag ggcgaggtcg tcttcgtccc caacccggcc taccccatcc
    10021 actcctattc tgtggtcatc gccggcgggg acttgaggac catcccgctt ctaccaaggg
    10081 aggaattctt cgaaaggctg aatatcgcgg tgaagaccac gtggcccctg ccgaagatgc
    10141 tcatcatcag ttttcccaac aaccccacca cggaggttgt ggaaaaggac ttcttcgaaa
    10201 ggaccgtcgc cttcgcgaag gagcatgaca tgatggtcgt ccacgacctg gcgtacgccg
    10261 atctcgcctt cgacggctac agggcgccga gcttcctcga ggtcccgggc gcgaaggatg
    10321 tgggcgtgga gttcttttcc atgtccaaga gctacagcat gcccggctgg cgggtcgggt
    10381 tcgcggcggg caacaaaagg atgataaacg ccctcagcag gataaagagc tacttcgact
    10441 acggcgtctt tcagccgatc cagatcgcgt ccatcatcgc cctcaatgaa ggcgacgcgg
    10501 atgtgcccga gatcgtggaa aagtacaggg cgcgacggga cgtgctttgc gagggcctct
    10561 gccgcctcgg gtgggaggtg gagaaaccga gagccacaat gttcgtctgg gcaaagatac
    10621 ctgagaagta cgcggcgatg gggtccatcg agttctcaaa gttcctcctg acagaggcaa
    10681 aggtggccac ctcgccgggg atcggcttcg gcgagttcgg tgaaggatac gtgcggttcg
    10741 ccctcgtgga gaacgagcac agaataaaac aggcggtgaa ggggatcagg aatctcctcc
    10801 gccctacggg aagatcatga tcggaatagg gataatagga ttgggaacag tgggaaccgg
    10861 cacataccgg atactgacgg aacacgccgc gctcattcgc aggaggacag gtctcgacct
    10921 tcgcgtcgtc agggtggccg aggtggacga gcgcaagctg agaggaaaag gccttgcacg
    10981 gaatgtcgtg acctccgatg ccatggagct catccatgac gagagggtcg gcatcgtcgt
    11041 ggagcttatt ggcggcatgc gtcccgccag cgacttcatc ggcgcggccc tcggtctagg
    11101 caagcaggtt gtgacggcaa acaaggccct cctcgcggaa aaaggcgacg agcttttcag
    11161 gctcgcggca aagaaggggt gcgagatcgg gttcgaggcc tccgtgtgcg gaggcatccc
    11221 cgttatccgg gcgatccgcg acggtctcgt cggcaatgat ttcagctaca tactgggaat
    11281 cctcaacggg acgagcaact acatcctctc gcggatgacc gacgaaggga tatccttcgc
    11341 cgaggccctc aaggacgccc agcggaaagg ctacgctgaa aaggacccca ccttcgacgt
    11401 cgaggggata gacgccgcgc acaagctttc gctcctggta aggctcgcct tcgcctcgcc
    11461 tgtcaccatg gacagaataa cgacacgggg catcgcgaag atagaccccg tcgacatagg
    11521 cttcgcccgg gacttcgggt acaagataaa gctccttgcc atcgcgaaga agcggggggc
    11581 aggcatcgag gctcgcgtcg agccagccat gctgcccgtg aaccacccca tgagcagcgt
    11641 caacggcgtc tataacgcgg tctacatagt cggggacatg acgggcccca atctcttcta
    11701 tggaaagggt gcggggagcg atcccacggg aagcgccgtc gtgagcgaca tcgtcgacat
    11761 cgccaagcgg atctccgggg cgggcggcac aggcgtgcgg cccctcgtcg cggcgggcag
    11821 gttcaaggaa ggcagggcct cttccgtgcc ctactacctg cgggtgatgg caaaggacat
    11881 gccgggtgtg ctttcgaaga tatcgggcat cctctcgaag tatcggataa gcatatcggc
    11941 ggtgacccag cagggccgga agctcagcgg ctacgtcccc gtcgtgatgg tgacccacga
    12001 tgcccccgag gaaggtctca tgaaggcgaa gaaggaaata gacgccctgt ccctgacgaa
    12061 gggggagagc gttcacataa ggatcgagga aggcaacctg tgagaggacc ggggccttcc
    12121 ggccgcgtga ggtgcggcag cgcatgaaat atatcgttct catcggcgac ggcatggcgg
    12181 attttccgct ggaggagctc ggggggaaga cgccgctcat ggccgcgaac aagccgaaca
    12241 tgaacgcggc ggcccgaaag ggtctcacgg gaaaggtaaa gacggtgccc gacggttttc
    12301 ctcccggcag cgacgtggcg ggtatgtcca tcttcggcta cgacccggcg ctgtattaca
    12361 cggggcgggc cccgatagag gccttcggca tgggcatccc catggggtcc gacgacgtcg
    12421 cctaccggtg caacctcgtt catatcggga ccgggaacgg gggtgatacc atgggcgact
    12481 actccggcgg gcatatctcc acggaggagg cacggatcct tgtcgcgtcc ctcaatgacg
    12541 agctgggttc cgaagggata accttcttcc ccggggtcgg gtaccgccac atcatgatat
    12601 ggaaaagagg cgcctgggag ttgtcgacaa cgcctcccca cgacatcacg ggcaaacaga
    12661 tcgcggggta cctccccgag ggcgacggag ccgcaatggt ccgttccctt atggaccgct
    12721 cccgcagggt gctcgggtcc cacgacatca acaggaagcg catcgacgcc ggcaagctcc
    12781 cggcgaacgg catatggctc tggggccagg gcaaaagagc cggcctgcca ccgttccggg
    12841 agaagttcgg ccttacgggg gcgacggtct gcgccgtgga cctcgtgcgg gggatctcga
    12901 gcctcgtggg gttcaccacc cccgtcgtcg aaggggccac gggatacctc gacaccgatt
    12961 acggggcgaa ggcgcgtgcg gccttgaaac tcctggaaga ccacgacatc gtgtacatcc
    13021 acgtggaggc gcccgacgag gcgtcccaca acggcagcat ccccgagaag ataaaggcca
    13081 tcgagcgcat cgacagggac gtgctcggcg tccttctcaa agaaacggac agggacacgc
    13141 ggttcctcat cgtcaccgac catgccacgc cgatcgcgat gaggacccac tacgccaccc
    13201 cggtgccctt cgccgtttac gacaggagcg atcccgcctc cgaagagggc ggtgattacg
    13261 acgagaacgc gggcggggcg gtgataagcg gtgaagagat gatacgtata ctgatcgggg
    13321 gaagacagtg agacaaaaat tcatcttcgt gacgggcggc gtcgtgtcgt ccctggggaa
    13381 gggcatagcg gcc
//
LOCUS       DAOQTR010000004        11106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_6595_length_11106_cov_3.640395, whole genome shotgun sequence.
ACCESSION   DAOQTR010000004 DAOQTR010000000
VERSION     DAOQTR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6595_length_11106_cov_3.640395"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..455
                     /locus_tag="PKN85_00240"
     CDS             21..455
                     /locus_tag="PKN85_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:66..68,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT42859.1"
                     /translation="MSDFDPQIIAFCCEYUGYTAADLAGSMRLSYPPSVKIVKVPCTG
                     RVDAIHILEAFEGGADGVCLVGCLEGDCHYISGNIRARKRVEYAKQLLDEAGIGGARL
                     EMYNLSSADGPRFARVAREITERIRELGPSPTRTVPGSRLET"
     gene            606..1274
                     /locus_tag="PKN85_00245"
     CDS             606..1274
                     /locus_tag="PKN85_00245"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNT42860.1"
                     /translation="MIVADRKPFDEIMGMIESCERILLVGCNECVTVCAAGGAKEVAL
                     LASQIRMKRKAEGRPVEVDEKTLERQCDPEYVNPLAETLEGYVAVLSIACGCGVQLIA
                     ETCRGLRVLPGLNTRFMGVTEEHGMWAERCQGCGDCVLDRTGGICPVTRCAKSIMNGP
                     CGGSQNGECELRQDTPCAWQLIIDRLKASGELSRYGEIAKPRDWSTSRDGGPRKRIRE
                     DMRI"
     gene            1271..2188
                     /locus_tag="PKN85_00250"
     CDS             1271..2188
                     /locus_tag="PKN85_00250"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNT42861.1"
                     /translation="MTAPGNLERVLTSGGFAVTAECGPPRGADPELIRKKGGYLKGFV
                     DAVNVTDNQTAVVRMSSMASCLILKEMGFDPVLQMTVRDRSRIALQSDILGAAALGIR
                     NILCLSGDHQSFGDHPGAKNVYDIDSIQFIDMARAMRDEGRLSSGAEITVRPEIFIGC
                     VENPFADPFDIRALRLAKKIGAGARFVQTQCVFNVEKFTRWMEMVRDLGLHEKAHILA
                     GITPMKSPGMARYMKNSVPGMDVPDHLVERMASVPKEGHREEGVAICLETIERVRAIE
                     GVAGIHIMAIEWEEIVPDIVRRAGLETRP"
     gene            complement(2313..3092)
                     /gene="rsmA"
                     /locus_tag="PKN85_00255"
     CDS             complement(2313..3092)
                     /gene="rsmA"
                     /locus_tag="PKN85_00255"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNT42862.1"
                     /translation="MLKRRFSQNLIKDGNILRKMVSLAGITAKDTVVEIGAGQGDLTR
                     AIASAAARVIAVELDREMIPHLEAACLAFPNIEIVSGDILGVDIAGLARGRTVKAMGN
                     IPYGITGPIIFKLIDERRSIESAYLTVQKEIGERMTANPGTRAYGSLSVASQIVADAK
                     VLMHVRAHVFIPPPKVDSIYLAMCFRDDADDVDDALLAFIRQSFSHKRKFMRHALLEV
                     FTEAEVETLYEKMGFPRSVRAETLEPRIFRKMYEVLKETRE"
     gene            complement(3103..4098)
                     /gene="tsaD"
                     /locus_tag="PKN85_00260"
     CDS             complement(3103..4098)
                     /gene="tsaD"
                     /locus_tag="PKN85_00260"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNT42863.1"
                     /translation="MLILGIDTSCDDTSVALMEEGRKIRSHIVSSQADLHSRFGGVVP
                     EIASRKHVEQIDVIYEEVLARAGASAEDVDLVGVTAGPGLIGSVLVGLCFAKGFCIAL
                     DRPLVGVNHIEAHAMSIFLEREVDFPFVALVVSGGHTIILLMEAPCRFRVLGSTRDDA
                     AGEAFDKIAKFLGIGYPGGRIIEEMARSGMRDYVAFPRPMMDDNTCDFSFSGLKTAFL
                     TYAKKHRIDDSTINHVLASFQEAICDVLVSKTVRAARLASVERIVVGGGVAANGRLRE
                     AFLERSRQEGLEVMISSPEFCTDNGAMVASLASFHAAAGMYSGLDIKGYSRMAIK"
     gene            complement(4113..>4433)
                     /locus_tag="PKN85_00265"
     CDS             complement(4113..>4433)
                     /locus_tag="PKN85_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42864.1"
                     /translation="TGFVSRTATSIPKLGEIPKDAMDLLGLTKEKPVYGKPVEMNGDY
                     YVFSFAGEQDPDRKEWEKNKEGFSRYYAARYREQFLRSFIEESKQAMLKDGKIKILKP
                     AKEL"
     assembly_gap    4434..4443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4444..5509)
                     /locus_tag="PKN85_00270"
     CDS             complement(<4444..5509)
                     /locus_tag="PKN85_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT42865.1"
                     /translation="MLKFMRKYARSYLIKIIFGVIIIVFVFYFGAGSIRERETVIAEV
                     GGFDVREEEYRVSYNQEVEMFRQLYRDKMDDKLLAELKEKVLSDIINKYILLREAKKL
                     GISVSDQEFADLIGGVEAFRKDGKFDKETYLAVLKQNRTDAEQFEKSQKTMLLLRKVV
                     SIIRDTGAPVSDAAIWAGYVREKGKVDLAYSRFDPAAYRDRVSVTDKELNDLYEKEKD
                     RFRGENTFRLKYIVIDGAGNLKDDQAYMDLIKAKDIDAYAKQKGLAVSDTGDVRQSEL
                     FRRFKSLKIETWVRDLRKGDISLPARDDGKSYIFQLVTREDGKPMEKSAALASLKARI
                     TSEKAKEMARLAAEDALKAKS"
     gene            5613..6653
                     /locus_tag="PKN85_00275"
     CDS             5613..6653
                     /locus_tag="PKN85_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNT42866.1"
                     /translation="MFESLFGFISKDLAIDLGTANTLVYVKSRGIVSNEPSVVAVHKD
                     SRGTKKVIAVGEEAKKMLGKTPGNIVAIRPLKDGVIADFEITEAMLKYFIQKIHNKKS
                     YARPRIVISVPSGITPVEKRAVKESAESAGAREVYLIEEPMAAAIGVGLPITEPSGNM
                     IVDIGGGTTEVAVISLAGIVYCNSVRVAGDKIDEAVIQYVKRKYNLLIGERTAELIKI
                     TIGSAYPSSEEEEMTMEIKGRDLVGGIPKTLEISSKEIREAINEPVNAIVEAVRIALE
                     RTPPELASDIVDKGIVISGGGAMLKNLDVLIKEVTRLPVIIAENPLTAVVEGAGKALD
                     EVSLLKEIATYF"
     gene            6666..7466
                     /gene="mreC"
                     /locus_tag="PKN85_00280"
     CDS             6666..7466
                     /gene="mreC"
                     /locus_tag="PKN85_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNT42867.1"
                     /translation="MRNSIAIIIAILVLVISFLSFTNAPFVARSYSTLKGGLGSVFGP
                     VLSVASKPVSAVGGFFDSYFNLVGAKKENEELREKYEKLYVENQKLVETERENARLRK
                     LLDFTQKRPNTMIAAGVIGEDLKNWFRCIIIDKGSKQNVRPKMPVVTPKGIVGQVVEV
                     DLWHAKVMVLNDTNSSIDVNVEGKNTRGLLEGTGQNTVKLKYVIKNDDIEIGDKLVTS
                     GKDGIYPKGIPAGIVITANKNKAGIFADIDVMPYNNFRQLEEVLLIKK"
     gene            7463..7954
                     /gene="mreD"
                     /locus_tag="PKN85_00285"
     CDS             7463..7954
                     /gene="mreD"
                     /locus_tag="PKN85_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNT42868.1"
                     /translation="MKTLVYIILGIVLLVIEAVISPHISLDVLKPELGMPIVLYVTFF
                     LGARSGLAAAVCIGLVEESLSGAPGGSLLFTTMFIYLIAMAMRRKFFVDSKYSFAYLS
                     SASVVVQSLLFLALTFFARGEARGALNILFYTIPNAIVTGFASILLFSLIEQLNETFL
                     ERN"
     gene            7954..9759
                     /gene="mrdA"
                     /locus_tag="PKN85_00290"
     CDS             7954..9759
                     /gene="mrdA"
                     /locus_tag="PKN85_00290"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNT42869.1"
                     /translation="MKDENLSSKYKWCTLVLVITMTVLLIRLWDLQIMRGSEMRKLSE
                     QNRIRVKKIVAPRGIIYDRTGKVLADTRPSFNIYLTPEDIRDFSQTVDGLARLLTCDR
                     EEIIEKMKAASGMPPSFPIKIKSDIPMDEVARVEAHRVYIPGVSIQIEPKRNYPYGAS
                     FAHVIGYVSEISDEELKNKKKYKDYSPGDYIGKYGLERSYENDLRGVDGEKRVEVDAI
                     GREVRTLDVVDPIPGHSLHLNLDLELQLSADKALEVRKGAAVALNPKTGGVLVLASRP
                     GFDPNLFASGISKKEWQKIALDKAHPLQNRAIQGGYPPGSTFKILTAMQALELGVINE
                     RTTFTCGGGFAYGNRVFKCWKKGGHGTTSVHRAIVESCDVFFYNVGLKLGVDRIHEFG
                     TAVGLGRVTGIDLPNEQKGLLPSTEWKKKRYNQPWYEGETVSVAIGQGAVWLTPVQLA
                     QLSSFVANDGKNFKPQIVNRIVSTEGKVVKTFEPVVNADVKFKTGVLKIVKDGMRGVV
                     NEPGGTAGASRLQNVLMCGKTGTAQAGSDKVKLGDHAWFIAYAPAEDASIAMAILVEH
                     GLHGSSAAAPIAKGITETLFKVKTVIKEAKADENR"
     gene            9746..10852
                     /gene="rodA"
                     /locus_tag="PKN85_00295"
     CDS             9746..10852
                     /gene="rodA"
                     /locus_tag="PKN85_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNT42870.1"
                     /translation="MRIDKRRYYHLDWYLIINGLLIFGIGLLNLLSATSSFSSGSYNF
                     MIKQLIAFAMGLVLIGVVMYYDYRVIANQSRWIYLGGLCLVILVLIIGLAAGGAKRWI
                     SLFGFSIQPSELMKPILVIFLANMLHEKKRQNIPLSLKDIGKPLLWTLIPFVLIVKQP
                     DLGTGIVIILVCFAILWYVGLKRSTYAVIFGVGAISPFFAWNYLMKPYQKMRVLSFIN
                     IDADPAGFGYHAKQAIIAVGSGRFLGKGFMNGTQHKLQFIPEHHTDFIFTVFSEEWGF
                     IGSVVLFALFISFINRCLKIAMNAHDELGSIMAFGIAAIIWVQFTINVMMAIHLAPVV
                     GIPLPFISYGGSSLLSMMISVGLLLNISTRRYMF"
BASE COUNT         2397 a         3258 c         3188 g         2253 t
ORIGIN      
        1 agtttttttg aagggagagc atgagcgact ttgaccccca gatcatagct ttctgttgcg
       61 agtactgagg gtatacagcg gcggacctgg caggttcgat gaggctttct tatcctccct
      121 cggtaaagat cgtcaaggtg ccctgcacgg gaagggtgga cgccatccac atccttgagg
      181 ccttcgaggg aggagcggac ggggtctgtc tcgtgggctg cctcgaaggc gactgccatt
      241 acatatcggg caatattcgg gcgcgaaagc gcgtggagta cgcaaaacag ctcctcgacg
      301 aagccggcat cggcggcgcc cgcttggaga tgtacaatct ctcctctgcc gacggtccca
      361 gattcgcccg ggtagcgcgg gagataacgg agaggatcag ggaactggga ccaagcccca
      421 ctaggacggt cccaggttcc aggctagaga cttgaagacg gtctcgggca gaagggactg
      481 tttcggatct cctgggaaga gtataaaatg gaaaactggt tctccaggta tgtatacagg
      541 ctatgaaata ggaccgtgag aggtttttag cgtgaaactt gaaaccgtct ttacaaggag
      601 cttttatgat cgttgctgac aggaaaccct ttgatgagat catggggatg atcgagtcct
      661 gcgagaggat ccttctcgtg ggctgcaatg agtgtgtcac cgtgtgcgcc gccgggggcg
      721 cgaaggaggt cgcacttctc gcctcccaga tccggatgaa gaggaaggcc gaggggcggc
      781 ccgtcgaggt cgatgagaag acgctcgaac gccagtgcga ccccgagtac gtcaatcccc
      841 ttgcggagac gctggaagga tacgttgcag tcctctccat cgcctgcggc tgcggtgtcc
      901 agcttatcgc cgaaacctgc cgggggcttc gggtccttcc cggtctcaac acacgattca
      961 tgggggtcac cgaggagcac gggatgtggg cggagcggtg ccagggatgt ggagactgcg
     1021 tcctcgacag gacaggcggg atatgccccg tcacgaggtg cgcgaagagc atcatgaacg
     1081 gcccctgcgg cggatcgcag aacggtgaat gcgagcttcg ccaagacacg ccctgcgcct
     1141 ggcagctcat catcgacagg ctcaaggcct cgggagagct ctcgaggtat ggcgagatcg
     1201 ccaaaccgag agattggtcc acgtcgcgcg acggagggcc cagaaagagg atacgggagg
     1261 atatgaggat atgacggccc cgggaaacct ggaaagagtg ctcacctcgg gcggtttcgc
     1321 cgttacggcg gaatgcggcc cgccgcgcgg ggcggacccg gaactcatcc gcaagaaagg
     1381 cgggtatctc aagggcttcg tcgacgccgt gaacgtcacc gacaaccaga cggccgtcgt
     1441 ccgcatgtcg agcatggcgt cgtgcctcat acttaaggag atgggattcg accccgtgct
     1501 tcagatgacc gtccgcgacc gcagccgcat agccctccag agcgacatcc ttggcgcggc
     1561 ggcgctgggt atccggaaca tcctgtgcct ttccggcgac caccagtcgt tcggcgacca
     1621 cccgggcgcg aagaacgtct acgacataga ctccatccaa ttcatcgaca tggcacgcgc
     1681 catgcgcgat gaaggcaggc tctcaagcgg cgccgagatc acggtgagac cggagatctt
     1741 catcggctgt gtcgagaacc cctttgccga ccccttcgat atacgggcac tgaggctggc
     1801 aaagaagatc ggcgccggcg cccgcttcgt ccagacccag tgcgtcttca acgtggaaaa
     1861 gtttacccgc tggatggaaa tggtccgcga ccttggcctt cacgagaagg cccacatcct
     1921 cgccggcatt acccccatga agtctccggg catggcgcgg tacatgaaga attccgttcc
     1981 cgggatggat gtgcccgacc acctcgtgga gcgcatggca tcggtgccga aggaaggcca
     2041 ccgcgaggaa ggcgtggcga tctgcctcga gaccatcgag agagtacggg ccatcgaagg
     2101 cgtggccggg atccacatca tggccataga atgggaggag atagtccccg acattgtaag
     2161 gagagcagga ctggaaacaa gaccgtgaag ggaaaagacc gtctcaggtt ccacgtttca
     2221 agttgaagat caaaagaaac acccacacgt cgcccttcgc atgcttgagg tgtctaacgt
     2281 ggaacctgag acctgagacc cgaaacggtt tcttattctc ttgtttcctt caagacttcg
     2341 tacatcttcc ggaagatccg gggctccagg gtttccgccc ttacggagcg gggaaagccc
     2401 attttctcgt agagggtttc cacctcggcc tcggtgaaga cctcaagaag ggcgtggcgc
     2461 atgaacttgc gtttgtggct gaaggactgg cggataaagg cgagcagggc gtcgtcgacg
     2521 tcgtccgcgt catcccggaa gcacatcgcc agatagatgc tgtccacctt cggcggcggg
     2581 atgaagacat gcgccctgac atgcatgagg acctttgcgt ccgcgacgat ctgcgaggcc
     2641 accgagagcg aaccgtaggc cctggtgccg gggttcgcgg tcatgcgctc gccgatctcc
     2701 ttctgcacgg tgaggtaagc actctcgatg gaccgccttt catcgatgag cttgaagatg
     2761 atgggtcccg tgatcccgta ggggatgttc cccatcgcct tgacggtccg gccccgtgca
     2821 agaccggcga tgtcgacgcc cagtatgtcg ccggaaacga tctcgatatt gggaaaagcg
     2881 aggcacgccg cctcgaggtg ggggatcatc tcccggtcga gctcgacggc gatgacccgc
     2941 gccgccgccg aggcgatcgc ccgtgtcagg tcgccctgtc cggcacctat ctccaccacc
     3001 gtgtctttgg cggtgatccc ggcgagggac accatcttgc gcaggatgtt gccgtccttt
     3061 atgaggttct gcgagaaacg ccttttcagc atggtcgtcg gatcacttta tcgccatccg
     3121 cgaatacccc ttgatatcga gaccggagta catgcctgcc gcggcatgga aggaggccag
     3181 cgacgccacc atggccccgt tgtcggtgca gaactcgggg gacgagatca tgacctcgag
     3241 gccctcctgc cgggagcgct ccagaaaggc ctcgcggagc ctgccgttgg cagcgacgcc
     3301 tcctccgacg acgatccgtt ccacactggc aagcctcgcg gccctcaccg ttttggacac
     3361 caggacgtcg catatggctt cctgaaagga ggccaggacg tggttgatgg tggagtcgtc
     3421 gatcctgtgc ttcttcgcgt aggtgagaaa ggccgtcttg agcccgctga aactgaaatc
     3481 gcaggtgttg tcgtccatca tgggacgggg aaaggcgacg tagtccctca taccgcttcg
     3541 ggccatctcc tctattattc tcccgccggg ataaccgatg ccgaggaact tcgcgatctt
     3601 gtcgaaggcc tctcccgcag cgtcgtcgcg ggtggagccg agcaccctga aacggcaggg
     3661 cgcctccatg aggaggatga tcgtgtgccc cccggagacg acgagagcaa cgaaggggaa
     3721 gtccacctcg cgctcgagaa agatgctcat ggcgtgggcc tctatgtggt tcaccccgac
     3781 gaggggcctg tcgagggcaa tgcagaaccc cttggcgaag cagaggccga cgaggacact
     3841 tccgatgagc cccggtcccg cggtgacacc gacgaggtcc acgtcttccg ccgaggcccc
     3901 ggcccgggcg aggacctctt cgtatatcac atctatctgc tccacgtgtt ttcgggacgc
     3961 tatttcggga accaccccgc cgaagcgact gtgcaggtcc gcctggctgg agacgatgtg
     4021 ggaccggatc ttccggccct cttccatcag tgccaccgac gtgtcgtcgc aggaggtgtc
     4081 gatgccgagg atcagcatgg gcgaggattc tgtcagagct ctttcgcggg cttgagtatc
     4141 ttgatcttcc cgtctttgag catggcctgt ttcgattcct cgatgaagga cctcaggaat
     4201 tgttccctgt accttgccgc gtagtaccgg gaaaaacctt ccttgttctt ttcccattcc
     4261 ttcctgtccg ggtcctgttc gccggcaaag gagaatacgt agtaatcacc gttcatctcc
     4321 acaggcttgc catagacggg cttctctttc gtgagaccca ggagatccat ggcatccttc
     4381 gggatctctc ccagcttcgg gatcgacgtg gccgttcgcg aaacgaaacc cgtnnnnnnn
     4441 nnngggactt ggccttgagg gcgtcctcgg cagcaagccg ggccatttcc tttgctttct
     4501 cgctcgttat cctggctttc aggctggcca gggccgcgct cttctccatg ggctttccat
     4561 cctcgcgcgt aacgagctgg aaaatgtagg acttcccatc gtcgcgcgcg ggaagggaaa
     4621 tgtcgccctt cctcaggtcc ctgacccacg tctcgatctt caaggacttg aatctcctga
     4681 agagttcgct ctggcgcacg tctcccgtgt ccgacacggc aagacccttc tgtttggcgt
     4741 aggcgtcgat gtccttcgcc ttgatgaggt ccatgtaggc ctggtcgtcc ttaaggttgc
     4801 ccgcaccgtc gatgactatg tatttcaggc ggaaggtgtt ctcaccgcga aagcggtcct
     4861 tttctttctc gtacaggtca ttcagttcct tgtccgtgac cgagaccctg tccctgtatg
     4921 ccgcggggtc gaagcgcgag tacgcgaggt ccacttttcc cttctccctc acatacccgg
     4981 cccagatggc ggcgtcggag accggcgccc ccgtgtccct gatgatgctg accaccttgc
     5041 gcaaaaggag catggtcttc tgtgacttct cgaactgttc cgcgtcggtc ctgttctgct
     5101 tcaatacggc aagatacgtt tccttgtcaa acttcccgtc cttccgaaag gcttcaacac
     5161 cccctatgag gtcggcgaat tcctggtcgc tcacggagat gccaagcttt ttggcctccc
     5221 tgagaaggat gtacttgttg atgatgtcgc tcaagacctt ttccttcagt tcggccagga
     5281 gcttatcgtc catcttgtcg cggtagagct gccggaacat ctcaacctcc tggttgtatg
     5341 acacccggta ctcctcctcc ctgacatcga aaccaccgac ctcggctata accgtctccc
     5401 tctcccttat ggaccccgcc ccgaagtaga agacgaagac aatgatgata acgccgaaga
     5461 tgatcttgat caggtagctt ctcgcatatt ttctcatgaa cttgagcatt ggttctatta
     5521 cctctaaaga ttattgattt tgaaacttta aaatagtata ttaaggctgt tttttcaata
     5581 acttttaaaa ttcgcgattg aggggagagt cgatgttcga atcactcttt ggatttatat
     5641 cgaaggatct cgcgatagac ctgggcaccg caaacaccct cgtctacgtg aaaagcaggg
     5701 ggattgtctc caatgagccc tcggtggtgg ccgtccacaa ggactcacgg ggcacgaaga
     5761 aggtcatagc cgtcggtgaa gaggcaaaga aaatgctcgg gaagaccccc ggcaacatcg
     5821 tcgccatcag gccgctcaag gacggcgtca tcgccgactt tgagatcacc gaggcaatgc
     5881 tcaagtactt catccagaag atacacaaca agaaatccta cgccagaccg cgcatcgtca
     5941 tctccgttcc ctcgggtatc acacccgttg aaaagagggc cgtaaaggaa tccgcggagt
     6001 ccgccggagc gcgcgaggtc tacctgatag aggaaccgat ggccgccgcc ataggcgtcg
     6061 gcctgcccat aacggagccg agcggcaaca tgatcgtcga catcggcggg ggcacgacgg
     6121 aggtggccgt cataagcctg gccggcatcg tgtactgcaa ctccgtacgc gttgcgggcg
     6181 acaagatcga cgaggccgtc atccagtacg tcaaacgcaa gtacaacctg ctcatcggcg
     6241 aaagaacggc ggagctgatc aagatcacca ttggatcggc atacccgagt tccgaagagg
     6301 aagagatgac catggagatc aagggaagag acctcgtggg cggcatcccg aagacgctgg
     6361 agatatcgtc caaggagatc cgggaggcca tcaacgaacc ggtgaacgcc atcgtcgagg
     6421 ccgtcaggat agccctggaa aggacaccgc cggaactcgc gtcggacatc gtcgacaagg
     6481 gtatcgtcat aagcggcgga ggggcgatgc tcaagaacct ggacgtcctc atcaaggagg
     6541 ttacccgtct tcccgtcatc attgccgaaa accccctgac ggctgttgtg gaaggcgcag
     6601 gaaaagcact tgacgaggta agccttctga aagagatagc gacatatttc taagatagtg
     6661 gcgacttgag aaactcgata gctataatca tagccattct cgttcttgtc atatcattcc
     6721 tctcatttac caacgcgcct tttgtggcaa ggtcctattc cacgctgaaa ggcggcctcg
     6781 gcagcgtctt cgggccggtg ctcagcgtcg cgagcaagcc cgtcagtgcc gtgggagggt
     6841 tcttcgattc ctacttcaat ctcgtcggag ccaagaagga gaacgaggag ctccgggaaa
     6901 aatacgagaa gctctacgtc gagaaccaga agctcgtcga aacggaaagg gagaacgccc
     6961 ggcttcgaaa actcctcgac ttcacgcaga aaagaccgaa cacgatgata gcggccggcg
     7021 tcatcgggga agacctcaaa aactggttcc gctgcataat catcgacaag ggaagcaagc
     7081 agaacgtcag gccaaagatg cccgtggtga cgccgaaggg catagtgggg caggtcgtcg
     7141 aggtggacct ctggcacgcg aaggtcatgg tgctcaatga caccaactcg tcgatagacg
     7201 tcaacgtcga gggcaaaaat acccgtgggc ttctcgaagg tacggggcag aacacggtca
     7261 agctgaaata cgtcatcaag aacgatgata tcgaaatagg cgacaaactc gtcacctccg
     7321 gcaaggacgg gatctacccc aaggggatcc ccgcggggat cgtcatcacg gccaacaaga
     7381 acaaggccgg gatctttgcc gatatcgacg tcatgccgta caacaacttc aggcagcttg
     7441 aagaagtcct gctcataaag aaatgaagac ccttgtttac atcatcctcg gcatcgttct
     7501 tctcgtcatc gaagcggtga tctctcccca catctctctc gatgtcctca agccggaact
     7561 ggggatgccc atcgtgctct acgtcacttt cttcctggga gcgagatcgg ggctcgccgc
     7621 cgcggtgtgc atcggcctcg tggaagagag tctttcgggg gctcccggcg ggtcgctcct
     7681 gttcaccacc atgttcatct acctcattgc catggcgatg cgcaggaagt tcttcgtcga
     7741 ttccaagtac agctttgcgt acctctccag cgcgtccgtc gttgtgcagt ccctgctttt
     7801 cctggccctc accttcttcg cccgcggtga ggcccggggt gcgctcaaca tactcttcta
     7861 caccatcccc aacgccatcg tcaccgggtt cgcgtccata ttgctcttct ccctcatcga
     7921 acagctcaac gagaccttcc tggagaggaa ttgatgaagg acgagaacct atcgagcaag
     7981 tacaaatggt gcaccctcgt cctcgtcata accatgacgg tcctcctcat ccggctgtgg
     8041 gacctgcaga taatgcgcgg ctcggagatg cgcaaactgt cggagcagaa ccgcatcagg
     8101 gtcaagaaga tcgtcgctcc acggggaata atctacgaca ggaccggcaa ggtccttgcc
     8161 gacacgcgcc cctccttcaa catctacctg accccggagg atatcaggga cttcagccag
     8221 accgtcgacg gacttgcccg ccttctcacc tgcgatcgcg aagagatcat agagaagatg
     8281 aaggccgcca gcggaatgcc cccttccttt cccatcaaga tcaagtccga tatccccatg
     8341 gatgaggtcg cccgcgtcga ggcgcaccgc gtgtacatac cgggcgtttc catacagata
     8401 gagccaaagc gcaattaccc ttacggggcg tcattcgccc acgtgatcgg ctacgtgtcg
     8461 gagataagcg acgaggagct caagaacaag aaaaaataca aggactattc cccgggcgac
     8521 tatatcggga aatacgggct cgaaaggtcc tacgagaacg acctcagggg ggtagacggg
     8581 gaaaagcggg tcgaggtgga cgccatcggg cgcgaggtac gcaccctcga cgtcgtcgac
     8641 ccgattccgg gacacagcct gcacctgaac ctggatctgg agcttcagct ctccgccgac
     8701 aaggccctgg aggtacgaaa aggtgccgcg gtggcgctta atccgaagac gggcggcgtc
     8761 ctcgtcctcg cgagccggcc gggcttcgat cccaaccttt tcgcctcggg gatatccaag
     8821 aaggaatggc agaagatagc cctcgacaag gcgcatcccc ttcagaacag ggccattcag
     8881 ggcgggtatc cgccggggtc gaccttcaag atactcacgg cgatgcaggc cctcgaactg
     8941 ggtgttatca acgagcgcac gaccttcacc tgcggtggcg ggttcgccta cggcaaccgc
     9001 gtcttcaagt gctggaagaa gggggggcac gggacgacct cggtccatcg cgccatcgtg
     9061 gaatcctgtg atgtcttctt ttacaatgtc ggcctcaagc tgggcgtgga ccgcatacat
     9121 gagttcggca ccgccgtcgg cctcggccgc gtgacgggga tcgacctccc caatgagcaa
     9181 aaggggctcc tgccgtcaac ggaatggaag aagaaaagat acaatcaacc ctggtacgaa
     9241 ggtgagaccg tttccgtcgc gatcggacag ggggccgtct ggctgacgcc ggtccagctg
     9301 gcgcagttgt cgtctttcgt tgcgaacgac ggcaagaact tcaaacccca gatcgtcaac
     9361 cgcatcgtgt cgacggaggg caaggtggtc aagaccttcg agcccgtcgt caatgccgac
     9421 gtgaagttca aaaccggggt tctcaagatc gtcaaggacg gaatgcgggg ggtcgtgaac
     9481 gagccgggag gcaccgcagg ggcctcacgc ttgcagaacg tgctcatgtg cggaaagacg
     9541 ggaacggccc aggccggttc ggataaagtg aaactcggcg accacgcctg gttcatcgcg
     9601 tacgcgcctg ccgaggacgc atccatcgcc atggccatcc tggtggagca tgggctccac
     9661 ggctcgtccg cggccgcgcc gatagccaag ggcatcacgg agaccctctt caaggtcaaa
     9721 acagtcataa aggaagcgaa ggcagatgag aatagataaa aggagatact accacctcga
     9781 ctggtacctc atcatcaacg ggctgctgat attcggcatc ggccttttga acctcctgag
     9841 cgccaccagt tcattctcca gcggctccta caatttcatg atcaagcaac tcatagcctt
     9901 tgccatgggc cttgtcctca tcggcgtcgt catgtactat gattatcgcg tcatagcgaa
     9961 tcagtctcga tggatctatc tcggagggct ctgccttgtc atactcgtcc ttatcatagg
    10021 gctcgcggcg ggcggcgcca agagatggat aagcctcttc gggttcagca tccagccctc
    10081 ggagctcatg aaacccatcc tcgtgatttt cctggcgaac atgctgcacg agaagaaaag
    10141 gcagaatatc ccgctgtccc tgaaggacat cggcaaaccg ctcctgtgga ccctcatacc
    10201 cttcgtcctc atcgtgaagc agccggacct cggcacgggc atcgtcataa tcctcgtctg
    10261 ttttgccatc ctgtggtatg tgggcctcaa gagatcgacc tacgcggtca ttttcggcgt
    10321 cggggcgata tcgccctttt ttgcctggaa ctacctcatg aagccctacc agaaaatgcg
    10381 cgtcctgagc ttcatcaaca tcgacgcgga cccggccggt ttcggatacc acgcgaaaca
    10441 ggccatcatc gccgtggggt cggggagatt cctcgggaag ggcttcatga acgggacaca
    10501 gcacaagctt cagttcatac ccgagcacca cacggacttc atcttcacgg tcttcagtga
    10561 ggaatgggga ttcatcggct ccgtagtcct cttcgccctc ttcatatcct tcatcaacag
    10621 gtgcctcaag atcgccatga acgcccatga cgaactgggc tccatcatgg ccttcggcat
    10681 cgccgccatc atctgggtcc agttcaccat caacgtgatg atggcgatcc acctcgcccc
    10741 cgtcgtgggc attccccttc ccttcatcag ttacggcggc tcgtccctcc tctcaatgat
    10801 gatatccgtc ggactcctcc tcaacatcag taccaggaga tacatgttct aaaagaccgc
    10861 ttgagtcgct tgaatggctt gaatcgcttg acaggctgtt gataaaccca ttttctacgt
    10921 cattccggcg aaggccggaa tccagaggtt tctctaaccc ctcgtttttc ctggattccg
    10981 gccttcgccg gaatgacgaa agaacacgaa tcgtcttcag aaatcacttt gtcaacagcc
    11041 tgttgagcga ttcaagccat tcaagccacc tctcttgtcc ttaagcccag atcggggcaa
    11101 taccga
//
LOCUS       DAOQTR010000005        10783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_6863_length_10783_cov_3.568139, whole genome shotgun sequence.
ACCESSION   DAOQTR010000005 DAOQTR010000000
VERSION     DAOQTR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6863_length_10783_cov_3.568139"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="PKN85_00300"
     CDS             complement(<1..1426)
                     /locus_tag="PKN85_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT42871.1"
                     /translation="MKKKLAQTVLTARGGLERKRLNMSATTETELYKTLANNLQVGVY
                     IAVNKRFQFVNLHIQEYTGYAEAELLQMDPLSVVHPDDRVTAVRETVDMLKGHRLTPF
                     EYRLITKQGQVKWIMETVTPIIFKKQKAVLGNSMDITEHKVAKESLQELDALKSSILD
                     AIPQAVVGLEDRRINFANTAVEEVFGWHPEELIGKSVTLFYRNETEAEEIGRYFYSTL
                     EHQRTFVAEFPCRRKNGRDILCRMRSARIGDALKQRRIVITYEDITEQRRAEQELANS
                     REQLRELSIYLQSVREKESTRIAREIHDELGQSLTALQMDLAWLGSSMPPGDLSLAGK
                     VQRMRTLVDGTIDSVHRISTELRPILLDDLGLIAAMEWQVGEFQGRTGVQCDARFDCK
                     DGSVDKDLATALFRIFQETLTNVARHSGATLVRVRFVEKGGNLHLEVTDNGRGITREE
                     RDNPKSFGIIGIQERVNLWGGNVSI"
     gene            1750..2322
                     /locus_tag="PKN85_00305"
     CDS             1750..2322
                     /locus_tag="PKN85_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT42872.1"
                     /translation="MDLFAAMRGRRSCRDYLPEAVGDDVIEKVLEAATWAPSPLNMQP
                     WEFFVITDAAVRERIYDEAQRCRLWAFEKSGWKWLESYRVDFLKQAPVIVAVVGDPGK
                     TGVDMFMEEGGMGYQAACAAAVQNMHLAAHSLGLGSLWFTLFDKKVLREILGIDGAKM
                     PLALVCLGKGASDIREVPRKAVKEKTTYLR"
     gene            complement(2396..2938)
                     /locus_tag="PKN85_00310"
     CDS             complement(2396..2938)
                     /locus_tag="PKN85_00310"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HNT42873.1"
                     /translation="METPQNPVHAQLEQLPRETLIELIKMYSWNWHTCDGLWFSGVED
                     RYGTEKALEIDIHMWDVSSKLEARRIKEVLSLPENAGLDAVLGAINLMSWAAKCSYII
                     QKDGERALLTVLSCPPQEARLRSGKEVFACRPTFEHGFTNVAAIIDSRVKVSCKYCPP
                     GSHPPDSWCQWEFTGPKTAQ"
     gene            3086..4780
                     /locus_tag="PKN85_00315"
     CDS             3086..4780
                     /locus_tag="PKN85_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNT42874.1"
                     /translation="MKNRNAMAILPLLAVYLVSVAAFLSMSGIAICAETVFRNVIVLV
                     ADGMGSTHTTLTRWYKGAPLSLDAMYVGGVRTYSADSLITDSAPAATALACGFKSTDK
                     SVGIMACAVTIPGVPDPGGSRCKPIASVLEGAKLSGRSVGLVATSNVQHATPAAFSSH
                     WPYRADYSEIARQQVYQDIDVVLGGGRQYLLPADKGGTRKDDENLVNVLRSKGYAFVV
                     NREEMLNAASPKLWGMFADDSLAYDLDRKSLSPDEPSLSEMTGKAMEVLSKNPKGFFL
                     FVEGSKIDWASHANDPVGVISDVLAFDEAVGTAVDFARRSGGTMVIALSDHGNGGMSI
                     GNRGTDKAYSRLRHERVVGPLRKVSLTGEGIEKALKDDRSEDNVRKVIGKYLGIDDLT
                     PGEMAAVRSAKPGKMNEATGPLVSTRSCVGWTTHGHTGEDLFFYYYGMDRPLPTMENS
                     DIARVMAKAMGFDLADADRRLFVPAEEVFKRIGASVTLDRRDPRNMVLIVESEGKRAE
                     LPLSKNTMRIEGRTKTTRTLEGITVLAPATGKVYIPRQAVEIFENVQGFKSSKVQE"
     gene            complement(4874..5623)
                     /locus_tag="PKN85_00320"
     CDS             complement(4874..5623)
                     /locus_tag="PKN85_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT42875.1"
                     /translation="MYSAPVIKKTFEVLSLIVDSKRPLGVTEVARTLALSKSTVFGIL
                     KALQEENLIVKSKSTKRYTIGEELFKIAKRVLQGGELSSIARPFLEKLVENVDETVFL
                     CVREDSVVKVIDTIETRKKLKISSPVGTRLPITASVFCKAFLAPMEDADIRDFLKEKG
                     LPRYTPNSITDLDRFIDEIRVTRKRHYSLDLEEYLMGVRAIATLVYANDYPVGAICIL
                     GFTGSMPTDKLPEMAQHLVNTARRISDRLSQ"
     gene            5873..7777
                     /locus_tag="PKN85_00325"
     CDS             5873..7777
                     /locus_tag="PKN85_00325"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNT42876.1"
                     /translation="MDRSVPAEAGENLLETAMKAGVHINASCGGRGVCGKCRVRVVGG
                     SLVSPVHPSVGEEGHAAGIRLACASSVTGDADIEIPLESQVDRSVLRRSTVMSDVDAG
                     NIDEALLGEYGREPLVERYGLEIPRPDIQDNISDAARIARALASKPECGESVALPLRV
                     MRGLGARLRDADWKVTVNVAMSGQREVTSVTAGSAEGSHYAIAVDIGTTTVAAQLLAC
                     DGGEGKRIILASSSDYNRQISFGEDVISRIMYAGKRNGLKKLQEVIVSTVNLLIGELL
                     GETGLDRDDVSHVVVAGNTTMMHLFYGIEPKHIMLAPYTPLTTVFPPVGCGEVGLETA
                     PHTRLFSVPGVSSYVGGDIVSGVALSGMTEREGVSLFMDIGTNGEIVLGNGQWLLCAS
                     CSAGPAFEGGGIQFGTRAAPGAIEKVRINPATLEPMILTVGRKKPLGICGSGLIDIVA
                     ELLLAGVIDRSGKFSADRKTKRVRDGSNGREYVLCFASETNIERDIVITEIDLDNIMR
                     TKAAVYAGCSVLLENGGLAFGDLDMVIIAGGFGHSIDIEKAIVMGLLPELPAERFRFM
                     GNASLGGARIAAVSRGFFEKAGQVARSMTNIELSNNAQFMDEFMAAMFLPHTDSRAFP
                     ETMKSLKDRQ"
     gene            7932..8702
                     /locus_tag="PKN85_00330"
     CDS             7932..8702
                     /locus_tag="PKN85_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT42877.1"
                     /translation="MPRVIAVAGKGGVGKTTIGGMLVRYLVEKVGKGPVLAVDADPNS
                     NLNEVLGVSVHATIGEAREKLKEDVPAGMSKDNWLEYKVQEAVIEAKGFDLLVMGRPE
                     GPGCYCAANSMAKKYIDALKGNYAFVVVDNEAGMEHMSRLVTQDVDILYVISDPTPRG
                     ILTVSRILSIIGELGLHIRKTVVIVNRVSGKDGGMLEKAARERGVEIGGIVRDDEDLV
                     RADAEGKSVFELPSASGSLVDAWAIFEGTMGPDGSAGL"
     gene            8716..9603
                     /gene="folD"
                     /locus_tag="PKN85_00335"
     CDS             8716..9603
                     /gene="folD"
                     /locus_tag="PKN85_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNT42878.1"
                     /translation="MTARIISGTEVAKQVRERIAREVLVLRERTGVTPGLVTILVGDN
                     PASVSYVTGKQRTARELGFHSVQEDQPAGISEERLLELIAKYNNDPAIHGILVQLPLP
                     KHIDETRVLNAIDPDKDVDGLHPMNLGRLMIGEARFYPCTPFGIRELLAMAGVQTKGA
                     EVAVVGRSNLVGKPISMMLVQKEANADATVTMCHTSTRDLASHTRRADILIVAAGRAR
                     AITADMVKDGAVVIDVGVNRIGVTPEGKAKLCGDVDFEAVKEKASVITPVPGGVGPMT
                     ITMLMQNTLKAAEMAAEGK"
     gene            9607..9822
                     /locus_tag="PKN85_00340"
     CDS             9607..9822
                     /locus_tag="PKN85_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42879.1"
                     /translation="MEGYSYEEKEDHAGRKVKVLGATFEEGKPEERGDWREKLASRDE
                     RLGYIRSAMRYWYSADWYGSEKRKQEA"
     gene            9863..>10783
                     /locus_tag="PKN85_00345"
     CDS             9863..>10783
                     /locus_tag="PKN85_00345"
                     /inference="COORDINATES: protein motif:HMM:NF003377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HNT42880.1"
                     /translation="MAFEIPKIKYTGKIREVQLGTGAKAVRIGGASCYPFHRFEGEMP
                     NLPRVAFEVWDSPPEEWQSWAMEPYGDVLSDPVAWARKCIDAYGVEMIVLQLRSADPN
                     GENKGVEEVVEVVKKVADAIDVPLLVWGTANDEKDGELLRRVADACEGKNVAVGPVSD
                     KNYKQIGAMAIGYSQVVMASSPIDVNLAKQLNILLGNLGVNEEKVVIDPTTGGLGYGL
                     EYTYSVMERDRMAALTQEDAKLQFPIICNLAPEVWKTRETRLTKDEDPKLGDEKRRSI
                     LMEAVTAMTLLVAGADIVVMRHPDSVFLVRE"
BASE COUNT         2312 a         2883 c         3449 g         2139 t
ORIGIN      
        1 tgatgctcac gttgcctccc cacaggttga cccgttcctg gatgcctatg atcccgaacg
       61 acttcgggtt gtctctctcc tcccgggtga tgcctcttcc gttgtcggtc acctcgaggt
      121 gaaggttgcc gcctttctca acaaagcgga cgcggacgag tgtcgctccg gaatggcgag
      181 ccacgttcgt caatgtctcc tggaatatgc gaaaaagagc ggtagccagg tccttgtcga
      241 cggagccgtc cttgcagtcg aaacgggcgt cgcactgaac gccggtcctt ccctggaatt
      301 ccccgacctg ccattccatg gcggcgataa gaccaaggtc atcgagaagt atgggccgaa
      361 gctccgtgga gatgcggtgc acgctgtcta tcgtcccgtc gacgagggtg cgcatccgct
      421 gcaccttgcc ggcaagcgac aggtctcccg gcggcatcga agaaccgagc cacgcaaggt
      481 ccatctggag ggccgtgagc gactgtccca gttcgtcatg tatctcccgg gctatgcgcg
      541 tgctctcctt ctcgcgcacg gactgaagat agatcgacag ttcccggagc tgttcccggg
      601 aattcgcgag ttcttgctcg gcccgtctct gctcggtgat gtcttcatag gttatgacta
      661 tcctgcgctg tttcagcgcg tccccgatgc gggcgctcct catgcggcag aggatgtccc
      721 ttccattctt gcgccggcag ggaaactcgg cgacgaaggt gcgttgatgt tccagggtgc
      781 tgtagaagta gcggcctatc tcttccgcct ccgtctcgtt ccggtagaag agggtgacgc
      841 ttttgccgat aagctcctcc gggtgccaac cgaagacctc ctcgacggcc gtgttggcga
      901 agttgatccg cctgtcttcg agaccgacaa cggcctgggg gatcgcatcc aggatggacg
      961 acttgagggc gtccaactcc tggaggctct cctttgccac cttgtgttcc gtgatgtcca
     1021 tcgaattacc gaggacggcc ttttgtttct tgaaaatgat gggcgtcacc gtctccatga
     1081 tccacttgac ctgcccctgc tttgttatga gccggtattc gaagggggtg aggcggtgac
     1141 ctttcagcat gtcgacggtt tccctgacgg ccgtcaccct atcgtcggga tggacgacac
     1201 tgagggggtc catctgaagg agttccgcct cagcgtagcc cgtgtactcc tggatgtgga
     1261 gattgacgaa ttgaaatctc ttgttcacgg cgatatagac gcccacctgg aggttgttgg
     1321 cgagcgtctt atagagttct gtttcggtgg tagccgacat gttcagccgt ttccgctcga
     1381 gaccgccccg agccgtcagt actgtctgtg cgagcttctt cttcatgatc attacctgcc
     1441 gcgtaaccgt tccgggccca aagcgccggg ttccggtcct cgggacgaga aacctcaaac
     1501 gcagaccttt cccgttgttg agccaccttc gttgtgggag tagtaaaggt gagggtacgt
     1561 ctcaacgcaa gccgatgaga gaagcaattt ggccggacgt tcatgtgcgc cccggcatca
     1621 tgttcaatat tacaatgccc cgccggctat gtcaatcttt tgccgtgatg ttaggatgtc
     1681 attgacacaa tcctgaccga ctgatactat cacggggagg agaatagaca cagcaaaaag
     1741 ggggatgaca tggatctgtt cgcggctatg cgcggaagga ggagctgccg ggattacctg
     1801 ccggaagcgg tcggagacga tgtgatcgaa aaggttctgg aggccgcaac atgggcgcca
     1861 tcaccgctca acatgcagcc atgggaattc ttcgtgatca cggacgcggc ggtgagggaa
     1921 aggatctacg acgaggcaca gaggtgccgg ctgtgggcct tcgagaaaag cggttggaag
     1981 tggctcgagt catacagggt cgatttcctg aaacaggcgc ccgtcattgt cgcggtggtg
     2041 ggtgatccag ggaagacggg cgtggacatg ttcatggaag agggcggcat gggataccag
     2101 gccgcatgtg ccgcggcagt ccagaacatg caccttgccg cccattccct gggactgggc
     2161 tcgctctggt tcaccctctt cgacaagaag gtcttgaggg agatactggg gatcgacggg
     2221 gcgaagatgc cccttgccct cgtgtgcctg ggcaaagggg catcggatat ccgtgaggtc
     2281 ccgagaaagg ccgtgaagga aaaaacgacc tacctcaggt gaggtggttc gctccggagg
     2341 gacacagaga gagggatcct cgccgcggat caggataagg gccggcccgt cgtcgttatt
     2401 gcgccgtctt cggtcccgtg aactcccact ggcaccagct gtcaggggga tgagaacccg
     2461 ggggacagta tttgcaggat acttttaccc tggaatcgat gatcgcggcg acattcgtga
     2521 agccatgctc gaaggtgggc cggcaggcga agacctcctt gcctgaccgg agtctcgcct
     2581 cctggggagg acaggaaagg accgtcagaa gcgctctttc gccgtccttt tggatgatgt
     2641 aggaacattt cgccgcccag ctcatgaggt ttatggcgcc gaggaccgcg tccaggcccg
     2701 cgttctccgg cagggaaagg acctccttta tccgccgggc ttccagcttc gaggacacgt
     2761 cccacatgtg gatatcgatc tcgagggctt tttccgtccc gtatctgtcc tcaaccccgg
     2821 agaaccatag gccgtcacag gtatgccagt tccatgaata cattttgatg agctctatga
     2881 gcgtctccct cgggagttgt tccagttggg cgtgaaccgg gttctgtggc gtttccatga
     2941 ttccctgacc tccttgttgt gatgggtgcg gaaatagtat gacacgatgt ggatgggaaa
     3001 tgaaaataat tcccaaaaag aaaggctcca aagtaatata tatgataccc gttcgggcga
     3061 tgattgtcag agggaggctg gagtgatgaa gaacaggaat gcgatggcga tactgccgct
     3121 gctggccgtg tatctcgtgt ccgtcgctgc ttttctttcg atgagcggca ttgcgatctg
     3181 tgcggaaacg gtcttcagga acgtcatcgt ccttgtggcg gatggaatgg gctccaccca
     3241 tacgactttg accagatggt acaagggagc ccccctttcg ttggatgcta tgtatgtggg
     3301 aggggtgcgg acctatagcg ccgattcgct gataaccgac tcggcacctg ccgcgacggc
     3361 cctcgcatgc ggcttcaaat ccacggacaa gtccgtgggc ataatggcct gcgcggtgac
     3421 catcccgggg gtccccgatc ccggagggtc ccggtgcaaa ccgatagcgt ccgtgctgga
     3481 aggagcgaaa ctgagtggca ggtccgtcgg tcttgtggcg acctccaacg tccagcatgc
     3541 gacgcctgcc gccttctctt cccactggcc ctacagggcc gattacagcg agatcgcgag
     3601 acagcaggta taccaggaca tcgacgtggt cctggggggc gggaggcaat acctgcttcc
     3661 cgcggataag ggtggtacgc gaaaggacga cgagaacctc gtcaatgtcc tccggtcgaa
     3721 aggctatgct ttcgtggtga acagggaaga gatgttgaac gcagcatccc cgaagctctg
     3781 gggcatgttc gcggatgact cgctggcgta cgatctcgac agaaagagcc tctcgccgga
     3841 cgaaccaagc ctgtcggaaa tgacgggtaa ggcgatggag gtcctgtcga agaacccgaa
     3901 aggcttcttc ctgttcgtgg aaggaagcaa gatagactgg gcctctcacg ccaacgaccc
     3961 cgtgggggtg ataagcgatg tccttgcctt cgatgaggcc gtcgggactg ccgtcgattt
     4021 cgcgcgcagg agtggtggta ccatggtgat cgcgctgtcg gaccatggaa acggaggaat
     4081 gtccattggg aaccggggga cggacaaggc ttactcccgt ttgcggcacg agagggttgt
     4141 cggtcccctc aggaaggtgt ccctcaccgg cgaggggatt gagaaagcgt tgaaggacga
     4201 caggtcagag gacaacgtga ggaaggtgat agggaaatac ctcgggatag acgatctgac
     4261 accaggggag atggccgctg tccgttccgc gaagcccgga aagatgaacg aggcgacagg
     4321 ccctctcgta agcacgcgaa gctgcgtggg ttggacgacc cacgggcata cgggtgaaga
     4381 cctttttttc tattactatg ggatggacag acctctgcca acgatggaga actccgatat
     4441 agcccgcgtt atggcaaaag cgatgggatt cgacctggcc gatgcggaca ggcggctctt
     4501 cgtccccgcc gaagaggttt tcaagaggat tggggcatcg gtgaccctcg accgcaggga
     4561 cccccgcaac atggtcctca tcgtcgaatc ggaggggaag agagccgagt tgccgctgag
     4621 caaaaatacc atgagaatag agggaaggac gaaaaccacc cgcaccctgg aaggcatcac
     4681 cgtcctcgcc cccgcgaccg gcaaagtcta catcccccgg caggcggtgg agatcttcga
     4741 gaatgttcaa gggttcaaaa gttcaaaggt tcaagagtag aaaggcagac tatgtgcccg
     4801 ggtcagtcct tgcgttgaca gccgaccact cttcgcggtc tttctcttga attcttgaac
     4861 tcttgaaccg tctttactgt gacagtctgt cggatatcct gcgggcggtg ttgacgaggt
     4921 gttgagccat ttccggcagt ttgtccgtgg gcatggagcc ggtgaagccg aggatacaga
     4981 tggctccgac gggatagtcg ttggcgtaga cgagggtggc tatcgccctg acacccatga
     5041 gatattcctc gaggtcgagg ctgtagtggc gtttccgtgt cacccgtatc tcgtcgatga
     5101 aacggtcgag gtcggtgatg ctgttcggag tgtatctggg caggcctttt tccttgagaa
     5161 agtcccggat gtcggcgtcc tccatggggg cgaggaaggc cttgcagaat accgatgccg
     5221 ttatcggcag ccgtgttccg acgggggacg atatcttgag cttcttgcgg gtctctatgg
     5281 tgtcgatgac cttgacgacg ctatcctcgc ggacacagag aaagaccgtt tcgtcgacgt
     5341 tctcgacgag cttctcgagg aaggggcggg cgatcgagct cagctcaccg ccctgaagga
     5401 ccctcttcgc gatcttgaag agttcctcgc ctatcgtgta gcgcttcgtg gatttgctct
     5461 tcacgatgag gttctcctcc tgcagggcct tgagtatacc gaacacggtg ctcttgctga
     5521 gggcgagtgt ccgagccacc tccgtgacac ccagcggtct tttgctgtcc acgatgagcg
     5581 aaaggacctc aaaggtcttc tttatgacgg gcgcgctgta cataattcac gatggtgaac
     5641 actatataat ataagggccc ttttgtcaac gttaatttgt tgataatact gctttaagaa
     5701 gggaggtatc caaatagccg gttggcattt atgatagtga acacgggcct tttgggaagc
     5761 cgaagcagga gcggccgccc tggaaattag ggtatgttca aagatgggag aatttggtac
     5821 aatgatgtgt ctaaagacca tgccaccaga atacagaata agatttctgc cgatggaccg
     5881 gagtgtgccg gcggaagcag gagagaactt actcgaaaca gccatgaagg cgggggtgca
     5941 tatcaatgcc tcctgcgggg ggaggggagt ctgcggaaaa tgcagggtgc gggtggtcgg
     6001 aggttcactg gtttcgcccg tccatccttc tgtcggtgaa gaggggcatg ccgcgggcat
     6061 ccgtcttgcc tgcgcctcca gtgtcaccgg tgacgccgac atcgagatcc ctctggaatc
     6121 gcaggtggac agaagcgttc tgagaaggtc gacggtcatg agtgacgtgg acgcggggaa
     6181 catcgatgag gccctcctcg gcgaatatgg cagggagccg ctggtggaaa ggtatggtct
     6241 tgaaatccct cgccccgata tacaggacaa tatctccgat gcggcgagga tagcgcgggc
     6301 cctggcgtca aagccggaat gcggggagtc cgtcgcgctt cccttgaggg tgatgcgcgg
     6361 tctcggcgcc aggctcaggg acgcggactg gaaggtcacc gtcaacgtgg ctatgagcgg
     6421 tcagagagag gtcacatccg tcaccgcggg cagcgccgag gggtcacatt acgcgatcgc
     6481 cgtcgatatc gggacgacga ccgttgccgc ccaactcctc gcctgcgacg gtggtgaagg
     6541 aaagaggatc atactggcat cctcctcgga ctacaacaga cagatcagtt tcggagagga
     6601 cgtcatcagc aggatcatgt atgcgggaaa gcgcaacgga ctcaagaagc ttcaggaggt
     6661 catcgtctcc accgtcaacc tcctcatcgg cgaactcctg ggagagacgg gtcttgacag
     6721 ggacgacgta agccacgtcg tcgtggccgg gaacacaacg atgatgcacc tcttttacgg
     6781 gatagaaccg aaacacatca tgcttgcccc ctataccccg ctcacgacgg tctttcctcc
     6841 ggtcggctgc ggcgaagttg gcctcgagac tgcgccccac acgcgccttt tttccgtacc
     6901 cggcgtctcg tcctacgttg ggggagacat cgtatccggt gtggcgcttt cgggcatgac
     6961 ggaaagggaa ggagtttcgc tcttcatgga tatcgggacc aatggcgaga tcgttctcgg
     7021 gaacggtcag tggctcctgt gcgccagctg ttcggccggt cccgcttttg agggtggagg
     7081 cattcagttc ggcacgcggg cggccccggg ggccatcgaa aaggtgcgca tcaatcccgc
     7141 caccctggaa ccgatgatcc tcaccgtagg caggaagaaa ccgctcggga tctgtggttc
     7201 cggactcatc gacatcgtgg cggagcttct tctcgcaggt gttatcgacc gcagcgggaa
     7261 gttctcggct gatagaaaga cgaagagggt gagagacggc tcgaacggcc gggaatacgt
     7321 cctgtgtttc gcctcggaaa cgaatatcga acgtgacatc gtgatcaccg agatagacct
     7381 ggacaatatc atgcgaacga aggccgcggt gtacgcgggc tgctcggtgc tcctcgagaa
     7441 tggaggcctt gcatttggcg atctcgatat ggttattatt gcagggggct tcggtcactc
     7501 catagacatc gaaaaggcga tcgtgatggg tcttctgccc gaactgccgg cagagcgttt
     7561 ccggttcatg gggaacgcgt ccctgggagg cgccaggata gcggccgtct cccgcgggtt
     7621 cttcgagaag gcgggacagg ttgcccggtc gatgacgaac atcgaattgt cgaacaacgc
     7681 gcagttcatg gatgagttca tggcggcaat gtttctcccc cacacggaca gcagggcctt
     7741 tccggagacg atgaagtccc taaaggacag gcaataagtg acaggtcata ggtaataggt
     7801 aataggaaga cagatgaaga cagacaatgg actacggcac acgcgccaag cggaggttgg
     7861 agaaagtttt ctcccatcac ctataaccca tcacccatca cctgcctccg caggaggcgc
     7921 aggaggcctc catgcctaga gtgatcgcgg tggcgggtaa aggcggggtc gggaagacga
     7981 ccataggcgg aatgcttgtc agatatctcg tggaaaaggt agggaagggg ccggtgcttg
     8041 ccgtggatgc cgatcccaac tccaatctca acgaggtgct gggcgtcagc gtccacgcca
     8101 ccataggaga ggccagggag aaactcaagg aggatgtccc cgcgggaatg agcaaggaca
     8161 actggttgga gtacaaggtc caggaggcgg tcatcgaggc gaagggcttc gatcttctcg
     8221 tcatgggaag gccggagggc cccgggtgtt actgcgcggc gaacagcatg gcgaagaagt
     8281 atatcgatgc cctgaagggc aactacgcct tcgtggtcgt cgacaacgag gcgggtatgg
     8341 agcacatgag ccggctcgtg acgcaggatg tggacatcct ttacgtgata tcggacccga
     8401 cgccgcgggg gatcctcaca gtgtcgcgga tcctgtcgat cataggcgag ctcggtcttc
     8461 atataagaaa gaccgttgtc atagtcaacc gcgtgagcgg gaaggacgga ggtatgctgg
     8521 agaaggccgc ccgggaaaga ggtgtcgaga taggcggcat cgtcagggac gatgaggacc
     8581 tcgttcgcgc cgacgcggaa ggaaagagcg ttttcgagct cccatccgca tcgggttcgc
     8641 ttgtggacgc ctgggccatc ttcgaaggga cgatgggtcc cgacgggagc gctggtctgt
     8701 gacgaaggag cgtatatgac ggcaaggatc atcagtggaa cggaggtggc gaaacaggtc
     8761 cgggagcgga tagcccggga ggtgctcgtg cttcgtgaaa gaacaggtgt cacgccgggt
     8821 ctcgtgacca tcctcgttgg tgacaacccc gcgtcggtga gctacgtcac cggaaagcag
     8881 agaacggcca gagagcttgg cttccactcc gttcaggaag accagccggc aggcatatcc
     8941 gaggaaagac tcctcgagct catcgcgaag tacaacaatg accccgccat ccacggcatc
     9001 ctcgtgcagc tgcccctgcc gaagcatatc gacgagacaa gggtcttgaa cgccatagac
     9061 cccgacaagg atgttgacgg tctgcatccc atgaacctgg gcaggctcat gataggcgag
     9121 gcgcgtttct atccctgcac gcccttcggc atcagggagc ttcttgccat ggcaggtgtt
     9181 cagacgaaag gtgccgaggt cgccgtcgtc gggagaagca acctcgtcgg caagcccatc
     9241 tccatgatgc tggtccagaa ggaggcgaac gccgacgcca cagtgacgat gtgccacacg
     9301 tcgacacggg accttgcgtc acacacaagg agggcggaca tcctcatagt cgcggcgggc
     9361 agggcaagag cgataaccgc cgatatggtc aaggatggtg ccgtcgtgat agatgtcggt
     9421 gtcaacagga taggcgtgac ccccgagggc aaggcgaagc tctgcggcga cgtcgatttc
     9481 gaggcggtga aggagaaggc ctccgtcatc acacccgtcc cgggcggtgt ggggccgatg
     9541 acgatcacca tgctcatgca gaacacgctc aaggcggctg agatggccgc ggaaggaaag
     9601 tgaggcatgg aaggatacag ctacgaagag aaggaagacc acgcgggcag gaaggtgaag
     9661 gtcctggggg ccaccttcga ggaaggcaaa cccgaggagc gcggagactg gcgggagaag
     9721 cttgccagcc gcgatgaaag gctcggctac atacgctccg ccatgcgcta ctggtacagc
     9781 gcggactggt acgggagcga gaagagaaaa caggaagcct gagggtggca tttgcatata
     9841 gctcaacaca ggggaggaag agatggcctt tgagattcct aagataaaat acacggggaa
     9901 gatccgggag gttcagctgg gcacgggggc gaaggcggtg cgcataggcg gcgcgagctg
     9961 ctatcccttc caccgtttcg agggcgagat gcccaatctc ccgagggtgg ccttcgaggt
    10021 ctgggacagt cctcccgagg aatggcagtc ttgggccatg gaaccatacg gagatgtgct
    10081 ctccgacccc gtcgcctggg caaggaaatg catcgacgcc tacggggtgg agatgatcgt
    10141 tctccagctg aggagcgccg atcccaacgg tgagaacaaa ggggtcgaag aggtcgtcga
    10201 ggtcgtgaag aaagtggccg atgccatcga cgtgcccctt ctcgtctggg gaacggcaaa
    10261 cgacgagaag gacggcgagc tcttaaggag ggtcgccgat gcctgcgagg gcaaaaatgt
    10321 tgccgtcggt cccgtttcgg acaagaacta caaacagata ggcgccatgg ccatcggcta
    10381 cagccaggtc gtcatggcat cctcacccat agacgtcaat ctggcgaagc agctcaacat
    10441 cctcctcggc aacctcggcg tcaacgagga gaaggtcgtc atcgatccca cgacgggagg
    10501 cctcggctac ggcctcgaat acacctattc ggtgatggaa cgcgacagga tggcggcgct
    10561 cacgcaggag gacgccaagc ttcagttccc catcatctgc aaccttgccc cggaggtctg
    10621 gaagacccgg gagacaaggc tgacgaagga cgaagacccg aagctgggag acgagaagag
    10681 gcgctccatc ctcatggagg ccgtcacggc gatgacgctc cttgtcgcgg gggcggacat
    10741 cgtcgttatg cgccatcccg acagcgtatt ccttgtcagg gag
//
LOCUS       DAOQTR010000006        10525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_7074_length_10525_cov_3.982617, whole genome shotgun sequence.
ACCESSION   DAOQTR010000006 DAOQTR010000000
VERSION     DAOQTR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7074_length_10525_cov_3.982617"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKN85_00350"
     CDS             <1..182
                     /locus_tag="PKN85_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42881.1"
                     /translation="LTPASLEGAEVLRRARREKTRPDLGAYQDPAVFASRYAEGRKRV
                     FFSGGADGGRIGVVG"
     gene            504..941
                     /locus_tag="PKN85_00355"
     CDS             504..941
                     /locus_tag="PKN85_00355"
                     /inference="COORDINATES: protein motif:HMM:NF039818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42882.1"
                     /translation="MKIPEYITKAEVQKFCKTLKIRDWTKLKTAKILPKEAKTVLAAV
                     NDTKMSIDPEEFRKGLEVELEHGLTFKDANVTNNHPLVTGKIVIAHLKESLDYYKLLE
                     VAELEGDLHKAIVAGDTKKIKKEYKKLALAKIALAKTEAAQVK"
     gene            1142..1552
                     /locus_tag="PKN85_00360"
     CDS             1142..1552
                     /locus_tag="PKN85_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42883.1"
                     /translation="MAWWVGVSAAGFSLPLEVYRIGSLKDVQARAASTNIPIVFVYSD
                     ENTRCGLARTATLDVMGELKGSAFVIYVSKSDSDRIPQSVIKAMNSPAAGKFIPKTVA
                     VNPKTGKVEMIIPYISDRNKRIEEIRKTKTLMPR"
     gene            complement(1927..2616)
                     /locus_tag="PKN85_00365"
     CDS             complement(1927..2616)
                     /locus_tag="PKN85_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT42884.1"
                     /translation="MWKKRSFLKVLIPVVLAGFLFSCAGAFVSPAARADFEAGLALFN
                     QGKFAEALPYFERAAAAEPDYYEAHLYLGRAYLNMKSFAKAVPPLRTAYRLSPQDFKG
                     QIVDILIDALLGAAFSEVRTGNYEGSLSYLRELMSVEPKAKKVKEDISNVLVAVATSL
                     LMKGDVRDAVKLFGEAVTENPNNAKAYLGLARALLKTGAIPDAIDAGRKALSLDPASN
                     EAFNLMKEMVR"
     gene            complement(2651..3241)
                     /locus_tag="PKN85_00370"
     CDS             complement(2651..3241)
                     /locus_tag="PKN85_00370"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNT42885.1"
                     /translation="MVKKLRHVTLGFALVFAACVTVNIYFPAAAIQKAADQIVDDVRG
                     TKDQKQPEEKKNDKQSSIIDPLRGLIGPREAHAQINVEVSTPAIRSLKQAMASNFGQL
                     KPFYDRGAIGENNMGFVEPRDTGALNLKDKADLNRVVSQENGNRNTLYNEIMKANNLG
                     GDSLPKIQRIFANSWRGKSQPGWWIQNDGGGWERKR"
     gene            complement(3261..5897)
                     /locus_tag="PKN85_00375"
     CDS             complement(3261..5897)
                     /locus_tag="PKN85_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42886.1"
                     /translation="MGKKRFILTLIILVASLAAGLVLLARTPLLLRAVAAVSGPLFGY
                     DMAAESFSFYPLKAEVKGFTIADIRGKNFIFTSSRVSVSSSPGSAFRGNVEKVLLKDA
                     KIRIRLGDRRETETDLSFIRKIPPVHLLTMENGEFTLTFKGSPGTITLKNIDLTVKGF
                     SPDHGGALAFTGTVVIDGPGGSKLRATGRLRGRLDLTGILPDMLGKGAIEIDIDEGST
                     GDVTLKGMKFTLPVVFEKDRIVISRAAALIETVTFAGEGRKADLTKGQLSMSALYNTR
                     AGTLSADNLQITMPGIGAVKGAGRLTVKGAMPFNAVLETGDLNFSSLFAMAKTLMTEE
                     EAKKWSVEGLGSLKARMEGSLGGKTRSVSGTLTMDIRKGGFSSPDGSKAAQGITGSVI
                     LNFSLPRGEDREGSVKISSQVSSGEYLWGRYYKDLRKEPSRLSSRADVTVSGKGGSRF
                     RGTFDLFNTGRYAYDGFVNAGEWGVHVTAQDVPVRRAFDLFAADYLAQTAAVLKGMEI
                     DGKLDADVSVTSAGKGLGTKGSVRLSAASLKLPAISLGVAGIDVDIPFDLASAGSGDG
                     ASLPPGPLGIVTITGFTKGAYSLPELKIPVTAYGNDVSAPGIISVPFYDGTVRVRTLT
                     AKDILGASPKLLFGVSMRDIDAQKLLSDLTGLAFPGKVKARFPEIAYQNGELLTEGKM
                     TVDIFGGTIEVADMYVKDLFLASRKIGGDILFRNIDLGKVTETIKVGRITGIVEGSVK
                     DLVVEYGQPSRFVFDVDTVRKWGQSQKVSVDAIENISILGTGSGGIGAVLKSGLNKFF
                     KEYPYSRIGIRCTLQNDNFNIRGKIVEGGTEYLIRRAFLRGIDVVNRDPQNMVSFKDM
                     QERVGRVFEKDKDGAGPTIKVN"
     gene            complement(5975..6400)
                     /locus_tag="PKN85_00380"
     CDS             complement(5975..6400)
                     /locus_tag="PKN85_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42887.1"
                     /translation="MKRERVLLLAALPVLMAAVLAMISVTADAQVFSKEVTVGELVAN
                     PSQYDQKNVTVNAYAFIVRQKKDRGGNPFILVSIFDPRDRKKVITAFGPGSTQARNGD
                     MIKLTGKFKARSKRGRYTYDNEIETTGDKVVVTAKGTGP"
     gene            6629..8896
                     /locus_tag="PKN85_00385"
     CDS             6629..8896
                     /locus_tag="PKN85_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42888.1"
                     /translation="MIASLISLLLVTGLSGIVAQTVLIRECLILYGGNELSIGVTIGA
                     WVVWEALGAFIGGRWPRGARGAAHAFIAAGLIFGIVFPASIYAIRAFKVIAGLPPEIS
                     LGITSIFGASMVIFLPAGLLHGFAFTAACRVYEGVKGPGGKAAGKVYFYEMAGTIAGG
                     ILVSYVLVTWLHSFRIAGLILVLMALACLAMAVSAATRAGKLLAALSLAIALGSAVFM
                     AAGLDGRLQLRSIAAQWHGREVVSYENSLYQNIAVTRATGQYTFFTDGLPAATIPVPD
                     IARAEEIVHIPLLTHGTPRNVLVLHGGAGGVIGEVLKYPTVRRVDYVEIDPAYLGTLV
                     RYPSALVRSELGDPRVILHYTDGRRFVKAASPDRRYDVVLLGVASPATLQANRFFTLE
                     FFREVRRILTDEGILVFTVPGSLAYYDRELRDINMSAVATAGEVFAHVAVVPGDQNIF
                     LASASARDIDLSPQRLVDRLNDLRLTTRLISLPHLTYRLDRERMEWFRRAVEPSRAEV
                     NRDLAPKGLYYTIAYINALHTPSMKGLFAAAGRRGIPVFLAALGLFAAGAFLLKNRHR
                     RIPALFVISTTGFVVMLLELSLIFVFQVAYGYVFHEIGMLLTMLMAGMAAGSMAAARR
                     VTTAGVSKAFMAAEASLASFCLLLFFLFTLTGHGAGAGGLSGRLLFFALLFVPGFFAG
                     MEFPLAVELFEDREGTGGSVGPVYAGDLAGGFIGGMLGGFFLFPLMGLAMSCLLFGAL
                     KAAGLLLLLLSGKRK"
     gene            8953..10059
                     /gene="amrS"
                     /locus_tag="PKN85_00390"
     CDS             8953..10059
                     /gene="amrS"
                     /locus_tag="PKN85_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNT42889.1"
                     /translation="MRRRDFLKVAALTPFVLAAGTNVLGQAARPAFHEALYYRKKGSN
                     KVVECLLCPRRCTLPDGATGFCRARKNISGRLYSLGYGSPCAAHIDPVEKKPFFHVLP
                     RTYSFSVASAGCNFRCQFCQNWQISQASPLETANTTLSPQHVVAAAVRKDCRSIAYTY
                     TEPTNFYEYMFDTAKTARGRGILNVAHSNGYINPEPLKALCRYMDAVNIDLKGFSEDF
                     YGKLCEGELAPVLLTIKTLKRSGVWVEITNLVIEGYNDDEKMVREMCRWVVKEVGRDV
                     PVHFSRFYPMYKLTGVSPTSVRTLERSRDAGFEAGLQFAYIGNVPGHPGENTYCPTCR
                     TMLIQRSGYNVLNVTLKNGTCPSCGTKIGGIWAT"
     gene            10056..>10525
                     /gene="amrB"
                     /locus_tag="PKN85_00395"
     CDS             10056..>10525
                     /gene="amrB"
                     /locus_tag="PKN85_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT42890.1"
                     /translation="MRKTYANKGFAARILMAALAACVLVLAACEALAQSAAPVRKPVF
                     AGSFYPADKVSLQTMVDGYLKDAQRVGDALPGVFAVIAPHAGYVYSGKTAAYSYNAAR
                     RKGITTVILLGSSHRSSFKGIALYPTGSWETPLGKVPVDSAMGRKMASLCEAVK"
BASE COUNT         1986 a         3236 c         3055 g         2248 t
ORIGIN      
        1 atctcacgcc cgcttcgctc gagggcgcag aggttttgag gagagcacgc agagagaaga
       61 ctcggccgga tctcggagcc taccaagatc cggccgtctt tgcatcccgc tacgcggaag
      121 ggagaaagag agttttcttt tcaggtggag ctgatggggg aaggatagga gttgtggggt
      181 gaggtagtgt agggcctgga ggatgattat aggacctatg actacagggg ggatgggaaa
      241 ggacaggttg ttaaggataa ggatattctt ttctctcccg ccgggacggc gggacgagaa
      301 ggccgccgga tcttggtagg ctccgagatc cggcggcaat cttttctctc ttttatcctc
      361 tctttgcggt ctctgcggac tcgagagagg agcgcaagcg acgaacgggc gagagataac
      421 gtcttctccc cttcgcatct tgtggaatcg cacgaaggtt gctacaatgc ctgtatcacc
      481 acatcctcaa ggagggggtt acaatgaaga ttccggaata tatcaccaaa gcggaggttc
      541 agaagttttg caagaccctg aagatcaggg attggacgaa gctgaagact gcgaagatat
      601 tgccgaagga agcgaagacg gtgctcgcgg cagtcaatga cacgaagatg agcatcgacc
      661 ccgaggagtt ccgcaagggt ctcgaggtgg agctcgagca tgggctgacc ttcaaggacg
      721 cgaacgtgac gaacaatcat ccccttgtca cgggcaagat cgtcatcgcc catctcaagg
      781 aatccctcga ctactacaag ctcctcgagg tggccgaatt ggaaggcgac ctccacaagg
      841 cgatcgtggc gggcgatacg aagaagatca agaaggagta taagaagctc gccctggcga
      901 agatagccct ggcgaagacc gaggcggcgc aggtgaagtg aagacggctt gagggtgaag
      961 acggcttgag ccgcttgaat ggcttgaggg ttgagggctg gtcaggtaca cggacagccg
     1021 gtggggctcg cccgggattt cggtggttag aggtctgccg gaggaaggtg actgactatc
     1081 ttcacaaagg atgtttgcga tgaagcatgt gttgcgtatt gtctgtgtga tgagttgcgt
     1141 tttggcttgg tgggttgggg tttcggccgc ggggttttcg ctgcctctcg aggtgtacag
     1201 gataggttcg ctgaaggatg tgcaggcgag ggccgcatca acgaacatac ccatcgtctt
     1261 cgtctattcc gatgagaaca ctcggtgcgg acttgcgcgg acggccacgc tggatgtgat
     1321 gggagagctc aagggcagcg cctttgttat ctacgtttca aagagcgatt cggatcgcat
     1381 ccctcaatct gtcatcaagg ccatgaattc ccctgcggcg gggaagttca taccgaagac
     1441 cgtcgccgtc aacccgaaga ccggcaaggt cgaaatgatc atcccctaca taagcgacag
     1501 gaacaagaga atagaagaga tcagaaaaac caagaccctc atgcccagat aatcgcatcg
     1561 gcagagtgaa ccacccttac cttttccctc tcagcggctt gttttgggca agcgaaaaga
     1621 gccgcttgaa tcgcttgaca gccttttgag aaagccggct cccggtcccg tttgtgttcc
     1681 gccgtcatcc cgtcacagcc tcagtccgtc atcccggcga aggccgggat ccaggagaga
     1741 ccaccaattc ggtattactc tacagactac agatgtcaga cctttgtcgc tgaccatatg
     1801 ttgtcgaatc ccgggcctct tttctgccga gagccgatct ttcctggatt ccggccttcg
     1861 ccggaatgac gtacaaaagg actttttcaa cggcctgtca agcggctcaa gcgattcaag
     1921 ccgtctttac ctcaccatct ccttcatcag gttgaaggcc tcgttgctcg ccgggtcgag
     1981 ggagagggcc tttcggcccg cgtctatcgc gtcggggatg gcgcccgttt tcaggagcgc
     2041 ccgcgcgagt cccaggtacg ccttcgcgtt gttggggttc tccgtgacgg cttcaccgaa
     2101 gagcttcacc gcgtcgcgga cgtcgccctt catgagtagc gatgtcgcca cggcgacgag
     2161 cacgttcgag atatcctcct tgaccttctt cgccttcggc tccacggaca tgagctccct
     2221 caggtacgac agggagccct cgtagttgcc cgtcctgacc tccgagaagg ccgccccgag
     2281 aagcgcgtca atgagtatat cgacgatctg gcccttgaag tcctgcggcg agaggcggta
     2341 ggcggtcctc aggggaggga ccgccttcgc gaagctcttc atgttgagat aggccctgcc
     2401 gaggtagagg tgggcctcgt agtagtccgg ctcagcggca gccgccctct caaagtaagg
     2461 gagggcctcg gcgaacttcc cctggttgaa cagggcaagg ccggcctcaa agtcggcgcg
     2521 tgccgccgga cttacgaagg cccccgcgca ggagaaaaga aagccggcca gaactacggg
     2581 gatgagaacc ttcaagaacg accttttctt ccacatctgg atgcacctcc ctctttcgcc
     2641 ggaccccttg ttatctcttc ctttcccatc ctccgccgtc gttctgtatc caccagccgg
     2701 gctgggattt cccgcgccag ctgttggcaa agatcctctg gatcttcggc agagagtccc
     2761 cacccagatt gttcgccttc atgatctcgt tgtacagggt gttgcgattg ccgttttcct
     2821 ggctgacgac acggttgagg tccgccttgt ccttgaggtt taacgcaccc gtgtcgcgcg
     2881 gttccacgaa acccatgttg ttctccccga tggcgcccct gtcatagaag ggtttgagct
     2941 ggccaaaatt gctcgccatc gcctgtttga ggctcctgat ggccggggtg gagacctcca
     3001 cgttgatctg agcgtgtgcc tctctcggtc ctatgagccc ccgcagggga tctatgatac
     3061 tgctctgttt gtcgttcttc ttttcctccg gctgcttctg gtccttcgta ccccgcacgt
     3121 cgtccacgat ctggtcggca gccttctgga tggccgcggc cggaaaatag atgttgacgg
     3181 tgacgcaggc ggcgaagacc agcgcgaaac ccaacgtgac atgacgcaac ttcttgacca
     3241 ttgtatcctc cttgagctct ctagttcacc tttatcgtcg gacccgcgcc gtccttgtcc
     3301 ttctcaaaca cccggccgac gcgctcctgc atgtccttga agcttaccat attttggggg
     3361 tccctgttca ccacatctat ccccctaagg aaggctcgcc ttatgagata ctcggtgccc
     3421 ccttcgacga tcttccccct gatgttgaag ttatcgttct gaagcgtgca ccgtatgccg
     3481 atgcggctgt acgggtattc cttgaaaaac ttgttgaggc cgctcttcaa gaccgcccca
     3541 atgccgccgg aaccggtgcc caggatggat atattctcga tggcatcgac ggacaccttc
     3601 tgggactgcc cccatttcct caccgtgtcg acgtcaaaga cgaaccgcga cggttgaccg
     3661 tactcgacga ccaggtcctt tacggagccc tcgacgatgc ccgttatcct gccgaccttg
     3721 atcgtctccg tcaccttccc gaggtcgatg ttcctgaaga gaatgtctcc gcctatcttc
     3781 cgcgaggcca ggaagaggtc cttcacgtac atgtccgcca cctctatcgt gcccccgaag
     3841 atgtcgacgg tcatcttccc ctccgtcagg agctctccgt tctggtaggc gatctcggga
     3901 aagcgcgcct tgaccttccc gggaaaggcg aggcccgtga ggtcgctcaa gagcttctgg
     3961 gcgtctatgt cccgcatcga gaccccgaac aacagcttcg gagatgcgcc gaggatgtcc
     4021 tttgcggtga gggtcctcac gcggaccgta ccgtcgtaga agggaacgga tatgatcccc
     4081 ggggccgata cgtcgtttcc ataggcggtg acgggtatct tcagttctgg caggctatag
     4141 gcgcccttcg tgaacccggt gatggtgacg atgccgagag gtcccggcgg gagggaagca
     4201 ccatctccgc tcccggccga cgcgagatcg aaggggatat ccacgtcgat gcctgccaca
     4261 ccaagggaga tcgcgggcag tttcaacgac gcggcggaaa gcctcacgct ccctttcgtg
     4321 ccgagtcctt tccccgccga tgtgaccgac acatcggcgt cgagctttcc gtcaatctcc
     4381 atgcctttca gcaccgccgc cgtctgggcg agataatcgg ccgcgaagag gtcgaaggcc
     4441 cttctcaccg gaacatcctg cgccgtcaca tgcacgcccc actcaccggc gttcacgaaa
     4501 ccgtcgtaag cataccgccc cgtgttgaag aggtcgaagg tccccctgaa ccgggaaccc
     4561 cctttgccgc ttaccgtcac atcggcccgg gaggaaagcc ttgagggttc cttcctgaga
     4621 tccttgtaat accgtcccca gaggtactcc ccggaggata cctgtgacga tatcttcacg
     4681 gagccctccc tgtcctcgcc gcggggaagg ctgaaattga ggatgacgga acccgtgatg
     4741 ccctgggccg ccttcgagcc gtcgggagag gaaaagcccc ctttcctgat gtccatggtg
     4801 agcgtccccg aaacggaccg ggtcttgccg ccgaggctcc cttccatccg ggccttaagg
     4861 gaacccagtc cttcgacgga ccacttcttc gcctcttcct cggtcatgag cgtcttcgcc
     4921 atcgcgaaga gactggagaa attgaggtcc cctgtctcaa ggaccgcgtt gaaaggcatg
     4981 gctcccttga cggtcagacg ccccgctccc ttcaccgcgc ctatgccggg catggtgatc
     5041 tgcaggttgt ccgccgagag tgtccccgcc cgggtgttgt acagggcgct catcgagagc
     5101 tgtcccttcg tgagatccgc cttgcgtcct tcccccgcga aggtcaccgt ctcgatgagc
     5161 gccgccgccc gcgagatcac gatgcggtcc ttctcgaaga ccacgggaag ggtgaacttc
     5221 attcccttca acgttacatc gcccgtcgag ccctcatcga tatcgatctc gatggccccc
     5281 ttgccaagca tatcggggag gatccccgtc agatcaagcc tgcccctcaa ccttcccgtc
     5341 gcccttagct tcgagcctcc cggcccgtcg atgacgacgg tgccggtgaa ggcaagcgcg
     5401 cccccatggt ccggcgagaa acccttcacg gtgaggtcga tattcttgag ggtgatggta
     5461 ccgggagaac ccttgaaggt gagggtaaat tccccgttct ccatggtcag aaggtggacg
     5521 gggggtatct tccggatgaa ggacaggtcc gtttcggtct ctctcctgtc gccgagacgg
     5581 atgcgtatct tcgcgtcctt gagaaggacc ttctctacgt tgccccggaa ggcggacccc
     5641 ggactggaag agacgctgac ccgggaactc gtgaagatga agttctttcc gcgaatgtcg
     5701 gcgattgtga accccttgac ctccgccttc aggggataga acgaaaagga ttccgccgcc
     5761 atgtcgtaac cgaaaagagg cccgctcacc gcggcgacag cgcgcaggag aagcggcgtc
     5821 ctggcaagga ggaccagccc cgccgcgaga gaggcaacaa ggataataag agtgagaatg
     5881 aacctttttt ttcccatcgc tcgttccgcc ggcattatag cacgaaggcc cgcgagaagt
     5941 cgcggtcagc gtacttccgg tcatgctgcg gtcattatgg gccggtgccc tttgccgtca
     6001 cgaccacctt gtcgcccgtc gtctcgatct cgttgtcgta ggtgtagcgt ccgcgcttcg
     6061 acctggcttt gaacttgccc gtaagcttga tcatatcccc gttgcgggcc tgggtgcttc
     6121 ccgggccaaa ggcggttatg accttcttcc tgtccctggg gtcgaaaatg ctgacgagta
     6181 tgaacgggtt cccgccgcgg tccttcttct gacgaacgat gaatgcatag gcgttgacgg
     6241 tgacattctt ctgatcgtac tgggaaggat tcgcgacaag ttcgcccacg gttacctctt
     6301 tggagaagac ctgggcgtcc gccgtcacgg agatcatcgc gaggaccgcc gccatcaaga
     6361 ccgggagtgc ggccagcaac aagactcttt ctcttttcac tatctgttcc cccttcgatc
     6421 tcgtttttta acctatatcc cgtttccggg gaagagtcaa taaaacctgg cttcttgcct
     6481 ttctcccctc cttccgctat ggtatgtaaa ggaaacagaa gagacgggtt atgggtgata
     6541 ggtcatagat gatgggtatg gatcaaagaa aggtctttcc cattatctat aacccattac
     6601 ctataacccg tcttctcaag gtccacgcat gatcgcatct ctcatatctc ttcttctcgt
     6661 gaccggactc tcggggatcg tggcgcagac ggtccttatc agggagtgcc tcatccttta
     6721 cgggggaaac gagctttcca tcggcgtgac catcggcgcc tgggtcgtct gggaggccct
     6781 tggcgccttt attggcgggc ggtggcctcg cggggcccgc ggggcggcgc atgcgttcat
     6841 cgccgccggc ctcatcttcg gcatcgtctt cccggcaagc atctatgcca tccgcgcctt
     6901 caaggtcatc gcgggtctcc ctccggagat atccctcggc atcacgagca tcttcggcgc
     6961 gtccatggtg atatttctcc ccgcgggcct tctccacggt ttcgcgttca cggcggcctg
     7021 cagggtctat gaaggtgtga agggccccgg gggcaaggcg gcgggaaagg tctatttcta
     7081 cgagatggcc ggtaccatcg ccggggggat actggtaagc tacgtccttg tcacctggct
     7141 ccattcgttc cggatcgcgg gcctgatact cgttctcatg gccctcgcct gccttgccat
     7201 ggccgtctcg gcggcaaccc gcgcgggcaa gctccttgcg gctttgagcc ttgccatcgc
     7261 gctcggctcg gccgtcttca tggccgcagg ccttgacgga cgcctgcagc tgcggtccat
     7321 cgccgcgcaa tggcacggtc gcgaggtcgt ctcctacgaa aattccctct atcagaacat
     7381 tgccgtgacg agggcaacgg ggcagtacac gttcttcacc gacggcctgc cggcggccac
     7441 catacccgtg cccgacatag cccgtgcgga ggagatcgtc cacatcccgc ttctcaccca
     7501 cggcaccccg cggaacgtcc ttgtccttca cggcggagcg ggcggcgtca taggggaggt
     7561 cctcaagtac cccacggtgc gccgtgtcga ttacgtagag atcgaccccg cctatctcgg
     7621 gacgctcgtc cgctatccct ctgcactcgt ccggtccgaa cttggcgatc cgcgggtcat
     7681 cctgcactac acagacggca ggcgcttcgt gaaggcggca tcccccgacc ggcgctacga
     7741 cgtcgttctt ctcggggttg cctcccccgc gacgcttcag gcgaacaggt tctttaccct
     7801 ggagttcttc cgtgaagtgc ggcggatcct caccgatgaa ggcatccttg tattcaccgt
     7861 tcccggctcc ctcgcctact acgacaggga actcagggac atcaacatgt ccgccgtggc
     7921 caccgccggc gaggtcttcg ctcacgttgc cgtcgttccg ggggaccaga acatcttcct
     7981 cgcctcggcg tcggctagag acatcgatct atcaccgcag agactggtag acagactgaa
     8041 tgacctgcgg ctgaccaccc ggctcatctc gctgccacat ctcacgtacc gtctcgacag
     8101 ggagcgtatg gaatggttcc gccgcgccgt cgagccctcc cgggccgagg tcaaccgtga
     8161 ccttgccccg aagggcctct actatacgat cgcatacata aacgcccttc acaccccttc
     8221 aatgaagggt cttttcgcag ccgccgggcg ccggggtatt cccgtattcc tcgccgctct
     8281 gggccttttc gcggccgggg cgttcctcct gaagaacagg caccggagaa tacctgctct
     8341 ctttgtcatc tccacgacgg gtttcgtcgt catgctcctt gaactgtccc tcatctttgt
     8401 cttccaggtg gcatacgggt acgtcttcca cgagatcggc atgctcctca ccatgctcat
     8461 ggccggcatg gccgccggca gcatggccgc ggcccggcgt gtcacgacgg caggggtatc
     8521 gaaggccttc atggcggcgg aagcctccct ggcatccttc tgccttctcc ttttcttcct
     8581 gttcactctc acgggccatg gcgcgggcgc gggaggcctt tcgggcagac ttctcttctt
     8641 tgccctcctc tttgtgccgg ggttctttgc cggaatggaa ttccctcttg ccgtggagct
     8701 cttcgaggat agagagggca ccggggggtc cgtagggcct gtctacgcgg gcgacctcgc
     8761 cgggggtttc atagggggca tgctgggagg cttcttcctc tttcctctca tgggcctcgc
     8821 aatgagctgc ctccttttcg gcgccctcaa ggccgccggg ctgcttcttc ttctgttatc
     8881 ggggaagaga aagtagtata atagcaagga attgggcggt attccgcagt ataccacggt
     8941 gacggtgcca cggtgagaag aagagatttc ctgaaggttg cagcacttac gcctttcgtc
     9001 cttgccgcag gaacgaatgt tctggggcag gcggcccggc cggccttcca cgaggccctc
     9061 tactaccgga agaagggctc gaacaaggtt gtcgagtgcc tcctgtgccc ccgccgctgc
     9121 acgcttccag acggtgccac gggtttctgc cgggcccgca agaacatctc cggcaggctc
     9181 tattccctcg gctacggctc gccctgcgcc gcgcacatcg accccgtgga gaagaaaccg
     9241 ttcttccacg tccttcccag aacgtattcc ttctccgtgg cgagtgccgg ctgcaacttc
     9301 cggtgtcagt tctgtcagaa ctggcagatc tcccaggcat ctcccctcga gaccgccaac
     9361 acaacgctgt cgccgcagca tgtcgtcgcg gccgccgtcc gaaaggactg caggagcatc
     9421 gcttatacgt atacggaacc gacgaacttc tacgagtaca tgttcgacac ggcgaagaca
     9481 gcccgcgggc ggggcatcct caacgtcgcg cactccaacg gctacatcaa ccccgaaccc
     9541 ctcaaggctc tttgcaggta catggacgcc gtcaatatcg acctcaaggg attttccgag
     9601 gacttttacg gcaagctctg cgaaggcgag ctcgcgcccg ttctcctcac gataaagacg
     9661 ctcaagcggt ccggcgtgtg ggtggagatc acgaacctcg tcatagaggg ctacaacgat
     9721 gacgaaaaga tggtgagaga gatgtgccgg tgggtcgtga aggaggtcgg acgggacgtg
     9781 cccgtccatt tctcccgctt ctaccccatg tacaaactga cgggggtctc gcccacatcg
     9841 gtgagaacgc tggagaggtc ccgcgatgcc ggcttcgaag cagggctcca gttcgcttat
     9901 atagggaacg tcccgggcca tcccggagag aacacctact gcccgacgtg caggacgatg
     9961 ctcatccaga gatcggggta caatgtcctc aacgtcaccc tgaagaacgg cacgtgcccc
    10021 tcgtgcggca caaagatagg aggaatatgg gcgacatgag aaagacgtat gcgaacaaag
    10081 gctttgcggc ccggatcctg atggcggcgc ttgccgcctg cgttcttgtc ctggcggcat
    10141 gcgaggcgct cgcgcaatcc gccgccccgg tgagaaagcc tgtcttcgcg ggctccttct
    10201 acccagcgga caaggtgtcc ctgcaaacca tggtggacgg gtacctgaag gatgcacagc
    10261 gcgtgggcga cgcgctcccg ggggtgttcg ccgttatcgc ccctcacgcg gggtacgtct
    10321 attcgggaaa gaccgcggcc tattcataca acgccgccag gagaaagggc atcacgacgg
    10381 tcatcctcct cggctcgagc catcgcagtt ccttcaaggg catagccctc tacccgacag
    10441 gcagctggga aacacccctc ggaaaggtgc ccgtcgactc cgccatgggc aggaagatgg
    10501 cctccctctg cgaggccgtg aaaaa
//
LOCUS       DAOQTR010000007        10481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_7120_length_10481_cov_3.566852, whole genome shotgun sequence.
ACCESSION   DAOQTR010000007 DAOQTR010000000
VERSION     DAOQTR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7120_length_10481_cov_3.566852"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..835
                     /gene="rplC"
                     /locus_tag="PKN85_00400"
     CDS             179..835
                     /gene="rplC"
                     /locus_tag="PKN85_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNT42891.1"
                     /translation="MGLGLIGKKLGMSQIFDEHGNVVPVTVIKAGPCYVVQKKTADKE
                     GYNAVQIGFDEVIKVQRVNKPDAGHFKKADVTPTQVLREFKVTPEDLEANAVGTVYSV
                     DIFEPGDFIDVTGTSKGKGFAGVVKRHGFKGAPASRGSHEFFRHGGSIGQNMTPGRTM
                     KGKKMPGHMGSKKITVQNLKIIDVRGDTNLLLVEGAVPGPAKGYVVIKRAVKKATATE
                     "
     gene            1050..1418
                     /gene="queD"
                     /locus_tag="PKN85_00405"
     CDS             1050..1418
                     /gene="queD"
                     /locus_tag="PKN85_00405"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNT42892.1"
                     /translation="MFTLCVKDSFAAAHRLVGYKDKCEELHGHNFAVEVYLSGDRLGD
                     DGMLVDFKTIKANIQKVLDVLDHKYINDIPFFAERASSAEYVAMYIFTEMEKLMGTER
                     VSVSRVTVWESEKACASYER"
     gene            1408..2217
                     /gene="folE2"
                     /locus_tag="PKN85_00410"
     CDS             1408..2217
                     /gene="folE2"
                     /locus_tag="PKN85_00410"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNT42893.1"
                     /translation="MNDRQKMADVQNMHDSREIEIDKVGVKNVKYPIIVLDRANGHQN
                     TIATIDMYVNLPHRYKGTHMSRFVEILHEYKNMINMKNFPDLLKEMKTRLDAEAAHMS
                     VCFPYFIKKEAPVSKTPSYMEYQCGFKGSMGADNTMTEFIVSVSVPINTLCPCSKEIS
                     EQGAHNQRGITKVDVYTKRFFWIEDLIDIVEKSASSDIYSILKREDEKFITEHAFDNP
                     KFVEDVVRDISERLSRDTNIRWFAIEAENFESIHNHSAYAYLERNNEGGNV"
     gene            2214..2678
                     /locus_tag="PKN85_00415"
     CDS             2214..2678
                     /locus_tag="PKN85_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42894.1"
                     /translation="MKKIMILCFALSLVCAGCAAFQKQGSEPAPASKTQGFNQAFYGF
                     PDVPVPKELDIVNDRSFVYETPTFKAGVIVFSGNVDPASVESYYKLNMAKNGWKYINS
                     FRYKDIVMNYAKEDKTCNIKISRGAFQTELEIWVGPADKGSFPKSAPPNGPK"
     gene            2665..2919
                     /locus_tag="PKN85_00420"
     CDS             2665..2919
                     /locus_tag="PKN85_00420"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNT42895.1"
                     /translation="MDRSKQVNVHTIESIFEMDMIKDALDKEDIPYTIKEHRDTAYDG
                     LFILQKGFASLYVEEGDRDRALAIVKRIRNLPYVAYSEDD"
     gene            2894..3610
                     /gene="yqeC"
                     /locus_tag="PKN85_00425"
     CDS             2894..3610
                     /gene="yqeC"
                     /locus_tag="PKN85_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNT42896.1"
                     /translation="MWHIQRTIDPSDLIEGVRFASFIGGGGKTTLIEHLARSCLERGK
                     SVAIATTTKIFIAEPFALFDDWMKTPRGAPFMHIGKTIEGPKLTALSSQEVEVLGHDF
                     DVVLIEADGAKGHPLKYPASYEPVIPSFSDRVFIVAGLDALFKPFGSAVFRHDLFSRE
                     TGTEPPRLVYPDIFIRFFQDDALLKSTAGFMRTIVLNKYDTSRPRHLAAVLMQKILDR
                     TGIEQGVITAPEYGIYYRIR"
     gene            3792..4007
                     /locus_tag="PKN85_00430"
     CDS             3792..4007
                     /locus_tag="PKN85_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42897.1"
                     /translation="MDKPVIASVTLAEIYLEQGHVERSIEVYAELVKREPFNEAYKKR
                     LAGLKKEWKTGQKKPGTLRKALGKKLW"
     gene            4001..5077
                     /locus_tag="PKN85_00435"
     CDS             4001..5077
                     /locus_tag="PKN85_00435"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNT42898.1"
                     /translation="MVMIRQARIERVQSLLEETGLDGCVLKGMDNIFYLTGFRGSEGS
                     LFITRGDVVLVVDFRYVTHAREVTRDIRIEEMRPKRDVINDLCTKYRISKLGFDGAHV
                     HYNVFKSWAENLVGISLVPMNNAIEEIRKTKEPEEITAIMAAIEAATKAFTDVLGLVK
                     PGSTEKEIADELDHAMRKRGAQGPSFDTIVASGPRGALPHAEPGDRKLKEGETVIIDY
                     GAAVAGYCSDETVTVCLGEIPDKLSEIYAIVNDARKLGIEKARAGMAIARLDAIVRGY
                     IEERGYGDFFRHGVGHGVGIAVHEAPGINSVANGILEENMVITVEPGIYLPNIGGVRL
                     EDMVLITEDSPRVLTHIRKDIIKM"
     gene            5120..5686
                     /gene="efp"
                     /locus_tag="PKN85_00440"
     CDS             5120..5686
                     /gene="efp"
                     /locus_tag="PKN85_00440"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNT42899.1"
                     /translation="MIVDTTDFRKGLRILQDGEPFTIVEFQHVKPGKGGAFVRTRMKS
                     LVTGNVLDKTFRSGDKVEVPEVEEKQMQFLYKEDTNYHFMDEATYDQIFIEEANLGDT
                     KNYLKESMVITVLFYKGRTIGVDIQNFVNLTITQTEPGIKGDTAQNATKPAVLETGYT
                     VQVPLFVEEGDVVRVDTRTGQYIERVQK"
     gene            5694..6314
                     /gene="tmk"
                     /locus_tag="PKN85_00445"
     CDS             5694..6314
                     /gene="tmk"
                     /locus_tag="PKN85_00445"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNT42900.1"
                     /translation="MLITFEGIEGSGKTTQIELLSDYLAGKGYGVVKTREPGGTRFGE
                     ALREVLLRPGMDVDSLSELLVFMAMRAQHVEEVILPALQDGKVVLCDRFVDATYAYQG
                     YGRKIDLGVIETLNRPVTKGIRPNLTILMNVTAERGLKRKAAASAMDRIEEEDISFHQ
                     RVKKAYEKLAKEDPKRFFVVDGSLKVEAIHQVVREKVGNVLKSYGV"
     gene            6304..7263
                     /locus_tag="PKN85_00450"
     CDS             6304..7263
                     /locus_tag="PKN85_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HNT42901.1"
                     /translation="MAFDGIIGHERQKRFLNFLMERGNIPHAFLFCGQEGIGKKQIAM
                     EFTRRLFCQAGTGCGVCRPCVKLDRGSHPDLVVIRNEGSIGIDDSRMIAKEVSEHPFE
                     QDKRVIIIDNAETMTNEAANALLKTLEEPPPYNHFFVITSSEREIPLTIRSRCTRVAF
                     SPLSREQLELYFRDRAGIDPDRAGLLASISFGSIGSGLFWLDEDNFSLRVRMAEALSG
                     RANGFVLASSLAEKASMTDRSASMYLTFLLSFFRDLFVRKITGGTSFITNTDLEDILE
                     GTSGDLPWIENSLKRIQETVRVMRYNVNRWLVFENLLIHVARE"
     gene            7267..8304
                     /gene="ricT"
                     /locus_tag="PKN85_00455"
     CDS             7267..8304
                     /gene="ricT"
                     /locus_tag="PKN85_00455"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNT42902.1"
                     /translation="MKSCYVNIDRLTGVLELQAVEDIKIGDFVISELDKGTCLGTVVS
                     EPVDTVREDLPKIGRKATEQEVRDHFQLKEKEKYAFDFCKRKIEDMGLPMKLLSTEYL
                     FGGTKLLFYFISENRVDFRELVKELAKEFKIRIELRQVGVRDEAKIVGGLGNCGNTVC
                     CRRFLNNFSIVSIKMVKEQGLALNPSKISGICGRLMCCLAYEYETYLSLKKNFPKIGK
                     RVKTPQGEGKVVKHNALGGTLSVQLDEGKEITLALKDISAAEQPRGAQPAAPVDKGKA
                     KTDNGRGQPKDRQQNPPDRGRQKNGGNREQTAHKQSNPGDRSIPRKNNGQKKDNGQTS
                     RPVANKEDTDQ"
     gene            8456..9988
                     /gene="metG"
                     /locus_tag="PKN85_00460"
     CDS             8456..9988
                     /gene="metG"
                     /locus_tag="PKN85_00460"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNT42903.1"
                     /translation="MDKKYYITTPIYYINDVPHIGHAYTTIAADIMARYKRLGGYRVF
                     FLTGTDEHGQKVEKAAALSGIHPREHADSMVGRFTSLWETLNISNTGFIRTTEERHRK
                     VVQHIFDKVREKGDIYLGEYEDWYCVPCETYYTEMQLKDGLCPDCLRKPEKLKEESYF
                     FRLSKYMQPLIDHLESHRDFVMPEIRYNEVASFVKGGLRDLSVSRTSFSWGIKVPDDE
                     RHIVYVWFDALTNYLTGIGFLEDEEEFRSFWPCDAHLIGKDILRFHAVYWPSFLMSYG
                     LEPPKHIFAHGWWTIEGQKMSKSLGNVVDPLDVVNTYGVDEFRFFLFREVPFGLDGDF
                     SKQAIVHRINGDLANDFGNLTSRTVTMIGKFLKGRIETPEKKGGADDHVETSAMRLIG
                     EYRKEMEVFAFHRALQNVFEIMSILNKYIDSEAPWKLAKEGDVRVRTVMFNLWNGIRI
                     AAVLLYPFMPGKSQLIWKALGIGKSIETCDLEEEMRFYTPSDIAPIDKIPPVFPRIEE
                     TV"
     gene            9989..10276
                     /locus_tag="PKN85_00465"
     CDS             9989..10276
                     /locus_tag="PKN85_00465"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNT42904.1"
                     /translation="MAFTSLQKTLAKVLKGYRMGDLESIRLFAMWDRIAGEKMASHCQ
                     PVRITRGILYVEVDDPLWLTQLKYMKIDIQAKIEEVLQKDSVKDIRFYLKQ"
BASE COUNT         2641 a         2948 c         2905 g         1987 t
ORIGIN      
        1 attgcatgag tcatagtcgt ataatcgggt tttcggtgaa aaagacaata cattaagctt
       61 tttcttatat ttttcccggg agggtcagga gccctccgaa aagcgcgtct gacggaattc
      121 atcttgactt tcggggagtt tttgactatg atgttacaat tttttggagg tgttcaccat
      181 gggattaggt cttataggca agaagctcgg catgtcgcag atatttgacg aacacgggaa
      241 cgtcgtcccc gttacggtca taaaagctgg tccgtgctac gtggtccaga aaaaaaccgc
      301 cgacaaggaa ggctacaacg ccgtccagat cggtttcgat gaagtgatca aggtgcagcg
      361 cgtcaacaaa cccgacgcgg gccatttcaa gaaggccgat gtgacgccca cgcaggtcct
      421 cagggagttc aaggtgacac cggaagacct cgaggcgaat gcggtgggta ccgtctattc
      481 cgttgacatc ttcgaaccgg gggactttat cgacgtcacc ggcacatcga agggcaaggg
      541 tttcgccggc gtcgtcaagc gccacggttt caagggggct cccgcgtcgc gcggctccca
      601 cgagttcttc cggcacggcg gttccatcgg gcagaacatg acccccggac gcacgatgaa
      661 gggcaagaag atgcccggcc acatgggttc caagaagatc acggtccaaa acctcaagat
      721 catcgacgtc cgcggcgaca ccaacctcct gcttgtcgaa ggcgcggttc ccggcccggc
      781 aaaaggctat gtcgttataa aaagagcggt gaaaaaggca acagccacgg aatagagcaa
      841 gaaggatcag gaagaacatc aggcggaagc tgtcaggctt ccgctttttt gtgcccgaga
      901 acaaggggcg atagaagcga tcggaaaaaa taggacctgg tcctattttc ctattcgtcc
      961 tatcaaaacc ccgcctctct ccccctgctc ccctgttccc tgtcccctgt ccttcttgac
     1021 tttcccccct tcagacgata tcttatggca tgttcacact ctgcgtgaag gattcatttg
     1081 cggccgctca ccgcctggtc ggatacaagg acaaatgcga ggaactccac ggccacaatt
     1141 ttgcggtgga ggtctacctg agcggcgacc ggctgggaga tgacggaatg ctcgtcgact
     1201 tcaagaccat caaggcgaac atccagaaag tcctcgatgt cctggaccac aaatatatca
     1261 atgacatacc gttctttgcc gagcgggcca gctcggctga atatgtggcc atgtacatct
     1321 tcactgagat ggaaaagctg atgggcacgg agcgggtgtc cgtaagcagg gtcaccgtct
     1381 gggaatctga aaaggcctgt gcaagctatg aacgatagac agaaaatggc cgatgttcag
     1441 aacatgcacg acagccgcga gatcgagatc gacaaggtgg gggtcaagaa cgtcaagtac
     1501 cccatcatcg ttctcgaccg ggcgaacggc catcagaaca ccatcgccac catcgacatg
     1561 tacgtgaacc ttccccatcg ctacaagggg acgcacatga gcaggttcgt ggagatcctc
     1621 catgaataca agaacatgat caatatgaaa aacttccccg acctcttgaa ggagatgaag
     1681 acgcgcctcg acgcggaagc tgcccacatg tccgtgtgct tcccttattt catcaagaaa
     1741 gaggcccccg tgtcgaagac gccgagctac atggaatacc agtgcggatt caaaggttcc
     1801 atgggagcgg acaacaccat gacggaattc atcgtttccg tctctgtccc cataaacacg
     1861 ctctgcccct gctccaagga gataagtgaa cagggggccc acaaccagag agggatcaca
     1921 aaggtggatg tatacacgaa acggttcttt tggatcgagg acctcatcga tattgtagaa
     1981 aaaagtgcga gcagtgatat atactctatc ctaaagagag aagacgagaa gttcatcacc
     2041 gagcacgcct tcgacaaccc aaaattcgtc gaggatgtgg tgagggacat atccgagaga
     2101 ttatcacggg atacgaacat tcgctggttt gccatcgagg cagagaactt cgaaagcata
     2161 cacaaccaca gcgcgtacgc gtatctcgaa agaaacaacg agggagggaa tgtatgaaaa
     2221 agatcatgat cctgtgtttt gctctgtccc tggtgtgtgc cgggtgcgcc gccttccaaa
     2281 aacaggggtc cgaacccgca ccggcctcca agacccaggg atttaaccag gccttctatg
     2341 gctttcccga tgtgcccgtt ccgaaggagc tggatatcgt caacgaccgc agtttcgtct
     2401 acgaaacgcc cactttcaag gcaggggtca tcgtcttcag cggcaacgtc gatcccgcct
     2461 ctgttgagag ctactacaag ctcaacatgg ccaagaacgg ctggaaatac atcaacagct
     2521 ttcgctacaa agacattgtg atgaactacg ccaaggaaga caagacgtgc aatatcaaga
     2581 tatcccgcgg ggcatttcaa actgagctcg agatctgggt cggtccggcc gacaagggat
     2641 cgttcccgaa atccgccccc ccaaatggac cgaagtaagc aggtaaacgt ccacaccatc
     2701 gagagcatct ttgagatgga tatgatcaag gacgctctcg ataaagagga cattccctac
     2761 accatcaagg aacacaggga caccgcctac gacggccttt tcatcctcca gaaaggtttc
     2821 gcctcgctct acgtggaaga aggcgacaga gaccgtgccc ttgccattgt caaacggatc
     2881 agaaaccttc cctatgtggc atattcagag gacgattgac ccatcagacc tcatagaggg
     2941 cgtcaggttt gcttccttca tcgggggagg cggcaagacg accctgatcg agcatctcgc
     3001 ccggtcctgc ctcgaacgag gcaagtccgt ggccatcgcc acgacaacga agatattcat
     3061 cgccgagccc ttcgcgcttt tcgacgactg gatgaaaacg ccccgtggcg cccctttcat
     3121 gcacatcgga aaaacgatcg agggacccaa gctgacggca ctatcgtctc aggaagtgga
     3181 ggtcctgggc cacgatttcg atgtcgtcct catcgaggcc gacggtgcga aaggccaccc
     3241 cctcaaatac ccggcgtcat acgaacccgt catcccctcc ttctcggaca gggtcttcat
     3301 agtggccggc ctcgacgccc tcttcaagcc attcggcagc gctgtcttcc gccatgacct
     3361 cttcagccgc gagacaggga ccgaacctcc gcgcctcgtc taccccgaca tcttcatccg
     3421 cttcttccag gacgacgccc tcctcaaatc cacggccggc ttcatgcgca ccatagtcct
     3481 caacaagtac gacacgtccc gcccccgcca tctcgccgcc gtgctcatgc aaaagatact
     3541 ggaccgcacg ggtatagaac agggcgtgat caccgcgcca gaatacggga tctactacag
     3601 gatacgatag tccccgggtc tcaggaagaa gacaggggga acagttccac atttcaggat
     3661 gaaatacagg agaactgtcc cactttggtc tttgactcga aacacgcaac ccggaacccg
     3721 caactgtctt ttgcctggac cagcctgatt ttccttgctt tttacccaat atattcatat
     3781 atttatgcct tatggacaaa ccggttatcg cgtccgtcac gctggcggag atatacttgg
     3841 agcaggggca tgtcgaaagg tccatagagg tctacgcgga actggtgaaa agagaaccct
     3901 tcaacgaggc ctacaagaag cgcctcgccg ggctgaagaa ggaatggaag accgggcaga
     3961 agaaaccggg aacactgcga aaggccctcg ggaagaaact atggtgatga tacgccaggc
     4021 acggatagaa cgggtacaga gcctcctcga agagacaggg ctcgacggtt gcgttctcaa
     4081 gggcatggac aacatcttct atctgacggg gttcaggggg tccgagggga gcctcttcat
     4141 cacccgggga gacgtcgtcc tcgtcgttga ttttcgttat gtgacgcacg ccagagaggt
     4201 gacccgtgac atccgcatcg aggagatgag gcccaagcgc gacgtcatca acgatctctg
     4261 caccaaatac aggatctcca agctcggctt tgacggggcc catgtccact ataacgtttt
     4321 caagtcctgg gccgagaacc tcgtcggcat cagtctcgtt cccatgaaca atgccataga
     4381 ggagatcaga aagaccaagg aaccggagga gatcaccgcc atcatggcgg ccatcgaggc
     4441 cgcgacgaag gcgttcaccg acgtcctcgg cctggtgaag ccgggcagca cggaaaaaga
     4501 gattgccgac gagcttgacc acgcgatgcg caaacgcggg gcacagggac cgtccttcga
     4561 taccatcgtc gcctcgggtc cgcggggcgc cctgccccat gccgagccag gcgacaggaa
     4621 gctcaaggaa ggcgaaacgg tcatcatcga ctacggtgcc gccgtggccg gatactgcag
     4681 cgacgagacg gtcacggtgt gcctcgggga gatacccgac aagctctccg agatatacgc
     4741 catcgtcaac gatgcccgca agctggggat cgaaaaggcc cgggcgggca tggccatagc
     4801 gcggctcgac gccatcgtga ggggttacat agaggaacgc ggctacggcg acttcttccg
     4861 tcacggtgtg ggccacggcg tgggaatcgc cgtccacgag gcacccggca tcaacagcgt
     4921 ggccaacggg atactggagg aaaacatggt catcaccgtc gaacccggta tctacctgcc
     4981 gaatatcggt ggcgtcaggc ttgaagatat ggtactaatt acggaagata gcccgcgggt
     5041 cctgactcac ataagaaaag acatcataaa gatgtagata cgcataagcg ggaggtttta
     5101 tgatctcagg aggaaacaga tgatcgtcga cacaacagac ttcaggaagg gtctcaggat
     5161 tctccaggat ggcgagccct tcaccattgt cgagtttcag cacgtcaagc cgggcaaggg
     5221 cggagccttc gtcaggaccc gaatgaagag cctcgtcacg ggcaacgtcc tcgacaagac
     5281 cttcagatcc ggtgacaagg tggaggtccc cgaggtggag gagaaacaga tgcagttcct
     5341 ctacaaggaa gacaccaact accatttcat ggacgaggcc acctacgacc agatcttcat
     5401 cgaggaggcc aatctcgggg acacgaagaa ctaccttaaa gaaagcatgg tcataaccgt
     5461 tctcttctac aagggcagaa cgataggcgt cgatatccag aacttcgtaa atctcaccat
     5521 cacccagact gaaccgggga tcaagggtga cacggcgcaa aacgccacga agcccgcggt
     5581 cctggagaca ggatacaccg tgcaggtccc tctctttgtc gaagaagggg acgtggtcag
     5641 ggtcgacacc cggacaggcc agtacatcga gcgcgtgcag aaataggaga cccatgttaa
     5701 tcacctttga aggcatcgag gggagcggca aaacaacaca gatagaactc ctgagcgatt
     5761 atctggccgg caaaggctac ggggtggtca agacgcgtga gccagggggc acgcgcttcg
     5821 gcgaagcctt gcgcgaggtg ctcctgagac ctggcatgga tgtcgactcc ctgtcggagc
     5881 tcctcgtgtt catggccatg agggcgcaac acgtggaaga ggtcatcctt ccggcgctcc
     5941 aggacggcaa agtggtgttg tgcgacaggt ttgtcgacgc aacctacgcc tatcagggat
     6001 acggcaggaa gatcgacctc ggggtgatag aaaccctgaa cagaccggtg accaaaggca
     6061 tacggcccaa tctcacgata ctcatgaacg tgaccgccga gaggggtctg aaacgcaagg
     6121 cggcggcatc cgccatggac aggatagagg aagaggacat ctcctttcac cagagagtga
     6181 aaaaggccta cgaaaagctg gcaaaggaag accccaagcg tttcttcgtc gtcgacggct
     6241 ctctgaaggt cgaggcgatc catcaggtcg taagggaaaa ggtgggaaac gtcctcaaga
     6301 gctatggcgt ttgacggcat cataggccac gagaggcaga aacgcttcct gaactttctc
     6361 atggagcggg gcaacatccc tcacgcattt ctcttctgcg ggcaggaagg catcggcaaa
     6421 aaacagatcg ccatggagtt cacgaggcgc ctcttctgtc aggcagggac gggttgcggg
     6481 gtatgccgcc cctgcgtgaa gctcgaccgg ggaagccacc ccgacctcgt tgtcatacgg
     6541 aacgaagggt ccataggcat agacgactcc cggatgatcg cgaaggaggt ttcggaacac
     6601 cccttcgaac aggacaaacg ggtcattatc atcgacaacg cggaaacaat gacgaacgag
     6661 gccgcgaacg ccctccttaa aaccctggag gagcccccgc cctacaacca cttcttcgtc
     6721 atcacctcct ccgagcgaga gataccgctc accatccgct cccggtgcac ccgcgtcgcg
     6781 ttctcgcccc tttccaggga acagctggaa ctctacttca gggacagggc cggcatcgac
     6841 cccgaccggg cgggacttct ggcctccatc tcctttggca gcatcggcag cgggctcttc
     6901 tggctcgatg aggacaactt ctcactgagg gtcaggatgg cagaggcgct gtcgggaaga
     6961 gcgaacggtt tcgtccttgc gtcatccctg gcagagaagg cgtccatgac ggacaggagc
     7021 gcttccatgt acctgacgtt cctcctttcc ttcttccgtg acctcttcgt gagaaagatc
     7081 accggcggga cgtcgttcat cacgaacacc gacctcgagg acatcctcga gggaaccagc
     7141 ggcgatctcc cctggataga aaactcgctg aaaagaatac aggaaactgt gcgcgtgatg
     7201 aggtataatg ttaaccgatg gctggtattc gaaaaccttc ttattcacgt cgcgagggaa
     7261 taggcaatga agagttgtta tgtaaatatc gacaggctga caggcgtcct tgaacttcaa
     7321 gccgtcgagg atataaagat cggtgatttc gtcataagcg aactcgacaa gggcacctgt
     7381 ctcggtaccg tcgtgtccga gcccgtggac accgtgaggg aagaccttcc gaagatcggc
     7441 aggaaggcca cggagcagga ggtgagagat cacttccagc tgaaggaaaa agagaagtac
     7501 gcctttgact tctgcaagag aaagatcgaa gacatgggcc ttcccatgaa gctcctgtcc
     7561 acggaatatc tcttcggcgg aacaaaactg cttttctatt tcatctccga gaacagggtg
     7621 gatttccgcg aactggtcaa ggagcttgcc aaggagttca agatacggat agagctccgg
     7681 caggtgggcg tccgcgacga ggcgaagata gtcggcggcc tggggaactg cggcaacacc
     7741 gtgtgctgca gacggttcct caacaatttc tccatcgtct ccatcaagat ggtgaaggag
     7801 cagggcctgg cgctcaaccc ctccaagata tccggcatct gcggcaggct catgtgttgt
     7861 ctcgcctacg agtacgagac gtacctgtcc ctgaagaaga acttccccaa gataggcaag
     7921 cgcgtcaaga cgccccaggg cgagggcaaa gtggtgaaac acaatgccct gggcggtacc
     7981 ctgtccgtcc agctcgacga gggcaaggag ataacgctgg cgctcaaaga catctcggcg
     8041 gcggaacaac ccagaggcgc ccaacccgcc gctcccgtgg acaaaggcaa ggcgaaaacc
     8101 gacaacggca ggggacagcc gaaagacagg cagcagaacc ctccggaccg cggcaggcaa
     8161 aagaatggcg gcaaccggga gcagactgcg cataagcagt cgaaccccgg cgaccggagc
     8221 atccccagaa agaacaacgg tcaaaagaaa gacaacggcc agacgtccag accggtagcc
     8281 aacaaggaag ataccgacca gtgaccagat tccaatgacc ggttcgccag acaaggaacg
     8341 gctggaatcc ggtcattata agtctcattg gttattgtaa tttggtcatt ggttattgta
     8401 agtttatgtg gttattgcag tctgattgct ggttattccc cctcggagat ctgtcatgga
     8461 taaaaaatac tacataacga cccccatcta ttacatcaac gacgtccctc acataggcca
     8521 tgcgtacaca acgatagctg ccgatatcat ggcccgctac aagaggctcg gcggttaccg
     8581 cgtcttcttt ctcaccggaa ccgacgagca cggacagaaa gtggaaaagg ccgccgccct
     8641 ttcgggcatc cacccgcggg aacatgcgga ttcgatggtc ggccgcttca ccagcctctg
     8701 ggaaaccctc aacatatcca acacggggtt cataagaacg acggaggaac gccacaggaa
     8761 agtggtccag catatcttcg acaaggtgcg ggaaaaaggc gatatctacc tcggtgagta
     8821 cgaggactgg tactgcgttc cctgcgagac atattacacc gaaatgcagc tcaaggacgg
     8881 cctgtgtccg gactgtctgc gcaagccgga gaagctcaag gaagagagct atttcttccg
     8941 tctctcgaag tacatgcagc ccctgatcga tcacctggag agccatcgag acttcgtcat
     9001 gcccgagata cgctacaacg aggtggccag tttcgtgaag ggcggcctgc gcgacctgtc
     9061 ggtgagcagg acaagcttca gctgggggat aaaggtcccc gacgacgaac ggcatatcgt
     9121 ctacgtgtgg ttcgacgccc tgaccaacta cctgacaggc atcggctttc tcgaggacga
     9181 ggaagagttc aggtccttct ggccctgcga cgcccacctt atcggcaagg acatactccg
     9241 gttccacgcc gtctactggc cgagcttcct catgtcctat ggcctcgagc ccccgaagca
     9301 catcttcgcc cacgggtggt ggaccattga gggccagaag atgtccaagt cactgggcaa
     9361 tgtcgtcgac ccccttgatg tggtcaacac atacggcgtg gacgaattcc gcttcttcct
     9421 tttccgcgag gtccccttcg ggctcgacgg tgatttttcg aagcaggcca tcgttcaccg
     9481 tatcaacggc gacctggcga acgacttcgg caacctcacg agccgcaccg tcacgatgat
     9541 cggcaagttc ctcaaagggc ggatagagac ccccgagaag aagggaggcg ctgacgatca
     9601 cgtcgagacc tcggccatgc ggctcatcgg agagtaccgg aaggagatgg aggtcttcgc
     9661 ctttcacagg gcactgcaga atgtcttcga gatcatgtcc atcctcaaca agtacatcga
     9721 ctccgaagcc ccctggaagc tggcaaagga aggggacgtc cgtgtcagga cggtcatgtt
     9781 caatctctgg aacggcattc ggatcgcggc ggtcctgctt tatcccttca tgcccggcaa
     9841 atcccaactc atctggaagg ccctgggcat cggaaagagc atcgagacat gcgacctcga
     9901 ggaggagatg cgcttctaca cgccttcaga catcgccccc atagacaaga taccgcccgt
     9961 ctttccccgc atagaagaaa cggtgtaaat ggcattcacg tcgctccaga agaccctcgc
    10021 gaaggtgctg aaagggtatc gcatgggcga cctcgagtcc atcaggctct tcgcgatgtg
    10081 ggacaggatc gccggcgaaa agatggcctc ccactgccag cccgtgcgga tcaccagggg
    10141 gatcctctac gtcgaggtcg acgaccccct ctggctcact cagctcaaat acatgaagat
    10201 cgatatccag gccaaaatag aggaggtcct tcagaaggac agcgttaagg acatccggtt
    10261 ctacctcaaa caatagaaac agttcgagag ttcgagagtt cgagagttcg agagttcgag
    10321 agaaagaccg caaacggatg cgggttgtca acacaactcg cgcaccagcg aaggtgtccg
    10381 ttgcctcttt tgcgcacact agctgaaggc gtttggtgcc tttttttacc cttgaactct
    10441 tgaactcttg aactcttgaa ccgcctttat cctggtctct a
//
LOCUS       DAOQTR010000008        10266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_7315_length_10266_cov_5.076682, whole genome shotgun sequence.
ACCESSION   DAOQTR010000008 DAOQTR010000000
VERSION     DAOQTR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7315_length_10266_cov_5.076682"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKN85_00470"
     CDS             complement(<1..680)
                     /locus_tag="PKN85_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42905.1"
                     /translation="MDIFIQIILLTVTTLTGYFILKELLSSGVWGLVGATCGLILGYA
                     ILKIELKLRDISLKIILGSLTGVVVSLFVANLFVSRLLLALIKDIPITLPIYILFYFI
                     MGYLGFVLGKEKSKTIDLSKVPLFDRLEEDKDIKLLDTSTIIDGRIADICETGFVQGT
                     LVIPQFVLYEIQHIADHQDSVKRTRGRRGLDVLHRLQKQTFLTVRIVDYDFPKLKDVD
                     TKLIALAK"
     gene            complement(685..1212)
                     /locus_tag="PKN85_00475"
     CDS             complement(685..1212)
                     /locus_tag="PKN85_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNT42906.1"
                     /translation="MFDVGEVAVYPGHGVGRIESIEEKEFSGTKQSFYIIRILDTDMT
                     IMIPIDGAKNAGLRSVIDACEVCKVYDILKDKNVVHDNSPWNRRYKEYMERIKSGSIY
                     EVATVLKELYNLRYWKELSFGEKKMFEMARNLITKELSMSLGTEELIVEQEIEEIFLA
                     NNNSQKGNTANHHKG"
     gene            complement(1369..1974)
                     /locus_tag="PKN85_00480"
     CDS             complement(1369..1974)
                     /locus_tag="PKN85_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT42907.1"
                     /translation="MEKRFSNELITVIEKGDLESVVQFISKGADVNNADQNGNTPLMY
                     ASMRGFADTARFLLDNGADVNARNAQGVTPLTYACRNNHVGVLRLLVSRGADVNVPDR
                     DGGTALTFASKSGNLEIVRLLVEHGADLNFRDHDGDTPLMCAINGKFAGIINFLISRG
                     ADLAVMNKHNKTALDLASEKGLLRIVNDIKFKLLERQKQVS"
     gene            2123..3526
                     /gene="gltX"
                     /locus_tag="PKN85_00485"
     CDS             2123..3526
                     /gene="gltX"
                     /locus_tag="PKN85_00485"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNT42908.1"
                     /translation="MVKSRFAPSPTGNLHIGGARTALFNYLYTKHHGGVFVLRIEDTD
                     VERSKDDYVKDILGGLSWLGIHWDEGPYYQMDRMDLYREHAYRLLAEGHAYKCYCTAE
                     MLEEKRKKALKEGGKPHYDRTCRDLLPDDASSEGKPFVIRFRSPVSGEVSFTDLIRGT
                     ITFKCEELDDLIIFRTDSTPTYNFTVVVDDALMGITHIVRGDDHINNTPRQILIYEAL
                     KYPVPQFAHVPLIHGKDRTRLSKRHGATSLLEYRSDGFLPEALMNYLARLGWAHGDQE
                     IFSQQELIEKFDLPNVGKSPSVFDMDKLTWLNGHYLKTLPAEDIAGRLLPFLQRMGIE
                     ASPDGRLVRIVVNLRERARTLKEMAQMAEYFFRDDFAFDETARVKFLTAATRPILVDF
                     AEGLKAIASTDEEESLKALIEGLTKKYNAKPVGIIQPVRVALCGRTVSPGIFEVIAIL
                     GKEAVEKRLTRAVESIQ"
     gene            3531..6866
                     /locus_tag="PKN85_00490"
     CDS             3531..6866
                     /locus_tag="PKN85_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT42909.1"
                     /translation="MKLARLEIFGFKSFLNRTVFQFGEGITSIVGPNGCGKSNIVDAI
                     VWVLGERGTKSLRVKDMGDVIFHGSNGKRPVNIAEVTLDLTEGDREFVVKRRIYRDGT
                     NEYSLNGKIVRLKDVQDAFLGTGLGTNSYAIIEQGRIEALISMKPAERRVVIEEASGI
                     TRFEEKKRDAVNRMEETTSNLERIEDIYREVTASFGKAEQEWERWKAYQELADRLHEI
                     DRQLLLDGYAKLRKRMTKVRERQADLEEEILQKEADRSTVREEFEAKESEFALTDNII
                     RQLEVDLKSKEKDMESRLLEIEYLGEEVKRLEASRKGLAEEESSIAAETARHEKEIDA
                     LKERSGALAAELEEEKGKEARTRDVAQGFKTNIEEHEKILEESRTKLFVSMSALTETR
                     NRIAQIERQAEERRKRMERQAAEVKELEMLLASLDAKRKALHDTLQEALREKESLSAR
                     ESAAAGEREGILEEMERQKALVESARNEKRVKEEFLRQLGGYIEGPEDAVRAEDRLAN
                     LIRTEESRIKALERFFSEELEYEVVKSDSVEALSLAVREREGNRVFFPPKGIFRKAGD
                     DVEMEVAWIADIGEALSRVAAGEEGIFINDDVYVDSRGIILTGGASKKIDVRQFREKL
                     KLEKELQTLSADVERHGSALAASRPRLEACDAKCRSARAAREAQEGKARALEKDLLMA
                     EAQWKSAADRLTALQAATESPDDAGPEVLESLVADRGRREEEKAAIEASMARLRETLA
                     GLKKEHEAAQSAWHAVTIEIERKMNMLKSISDNRERSEAAIRSLADKTQGLKEKYAAI
                     DRDIEGRTQKIRYLERSYEELQADLQKHVARFEELKTMLGNLHAQKQTLQESIDTLGK
                     EIERVRARKENAEKDLAVLEEKRLTIVEHLVGTYGIDQPDEMSVQLPADIEEERESLT
                     ARIAEMGEINFRAEKEYLELKERISFLDKQKEDLLTAMESLRKTITKIDQFTKELFTE
                     TFDKVGSAFSRFTDLLFKGGKGHLAINAEQGGVDLFVQPPGKKVTRMELLSGGEKALI
                     SLAFLLALMDTRPSPFALMDEIDAPLDDANLAGLLDIIRGMSLKSQIVFITHNRITME
                     YSDMIYGITMEEQGISKTVSVRL"
     gene            7345..8916
                     /locus_tag="PKN85_00495"
     CDS             7345..8916
                     /locus_tag="PKN85_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42910.1"
                     /translation="MNPITYLDFQKPSTEMAIWGSIGVGLMLLISIILGFMSKRKVAK
                     GSFEEYLVGKRDIGPVVTGCALAASYLSGWAFAGSTGVVYAVGFSGMWFAGIWSLLGL
                     IPCCWLAAVKTASFSKKLGAATLPETIGRRFESKMLQTVVAICMLYFLFMYSVGQLKA
                     AGGVWYATTGLPAVWCLLLSIFIAWLYMVLGGYVGTQWSMAFQGAFLGFVGALLGIWA
                     IIWAGGFYEISAKLYMEPKVGPALIKLIRPELPKVGPTQLFSSMVGILATPVIFFTMA
                     IGFPHNVSRFLGMGTLNKKGYWTLIIMVYLIAGVPIMLDCSSNGLVARMIYGPNLLKV
                     KPWAADLAAPELAFAVGGVPMMTLYVMGLFAAALSTLAGMVFIMSANITRDVIKLWWP
                     QVSDKSMLYLGYFLIALFLFLPFYWTLVNPPPLLSIFMGLAAMGLGAIFVFVTAASYY
                     WKGATKWGALCCVLYGTFGSMYGGYKVLFKKEVGLGTFEWWLVIGCGILYFVVSAITK
                     KPSQELLDKLFPAKS"
     gene            complement(9108..9536)
                     /locus_tag="PKN85_00500"
     CDS             complement(9108..9536)
                     /locus_tag="PKN85_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT42911.1"
                     /translation="MRYFFAVFLTCVLVICGGCSSDEENTTTARDYYRQAYQFIDAGH
                     PREAIDLLSLAIKKDPNYYEAYYNRGVVFYLLKDYRRAADDFSKAIAIDPKNSSAYAS
                     RGEVREIMKESEKAYSDYRTAARMGNSEAQKYLASKGLSW"
     gene            complement(9660..10025)
                     /locus_tag="PKN85_00505"
     CDS             complement(9660..10025)
                     /locus_tag="PKN85_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNT42912.1"
                     /translation="MRKTVMIAMFFAAVSLLCSPAGALAFNAQDLQKLNTTNKCSKCD
                     LSQANLEGIDFYGANLNGANLSGANLKGTLFNDADLRGANLKGALTDKETNFSGAKLS
                     EAVWMDGSKCESGQIGRCK"
BASE COUNT         2335 a         2893 c         2917 g         2121 t
ORIGIN      
        1 cttttcgcga gggctataag cttagtgtcg acgtccttca gcttggggaa atcgtagtcc
       61 acgatcctga cggtgaggaa ggtctgcttc tgcagcctgt gcaggacatc gagtccccgg
      121 cggccgcgcg tccttttcac ggagtcctgg tggtcggcga tgtgctgtat ctcgtagagg
      181 acgaactggg gaatgacgag ggtcccctgc acgaagccgg tctcgcatat gtccgctatc
      241 cgcccgtcta tgatggtgct cgtatcgagg agcttgatgt ccttgtcctc ttccagacgg
      301 tcgaacagag gaaccttgga gaggtcgatc gtcttcgatt tctccttccc gaggacaaac
      361 ccgaggtagc ccatgatgaa gtagaagaga atgtaaatgg gaagcgtgat ggggatgtcc
      421 ttgatcaggg cgagaaggag acgggagacg aaaaggtttg ccacgaaaag gctcacgaca
      481 accccggtga gacttccgag aatgatcttc agggatatgt cccgaagctt cagctcgatc
      541 ttcagtatgg catacccgag gatgagcccg cacgtcgccc cgacgaggcc ccagactccg
      601 ctcgaaagaa gttctttaag tatgaaatat ccggtaaggg ttgtgacggt caggaggatt
      661 atctgaatga atatatccaa ggactcaacc cttgtggtga ttggcggtat ttcctttttg
      721 actgttgttg ttggccagga agatctcttc gatctcctgc tccactatga gctcctcggt
      781 gcccagagac atggaaagct ccttggtgat gagattgcgc gccatctcga acatcttttt
      841 ttcgccaaag gaaagctctt tccagtaccg gaggttgtag agctctttca ggaccgtcgc
      901 gacctcatag atggagccgc tcttgatcct ctccatgtat tccttgtacc gcctgttcca
      961 gggcgagttg tcgtgaacaa cgttcttgtc cttgagaata tcgtagacct tgcacacctc
     1021 gcaggcatcg atgacgcttc tcaagccggc attcttcgcg ccatcgatag gtatcatgat
     1081 ggtcatgtcc gtatcaagga tgcggattat gtaaaatgac tgtttggtac ctgagaactc
     1141 tttttcttcg atagactcga tccgaccgac accatgcccc ggatatactg caacctctcc
     1201 tacatcaaac attatgcctc cgaatagttt ttcatcgttt ttcggccagg ttgccatatt
     1261 gtagcataat attgagaaaa attaaataag taatggttat tatgtggtaa tcggcaggta
     1321 atccaaggct tcgcccctct ctctcgggag agaagcggta aacggggctc aggacacctg
     1381 cttctgcctt tccagaagct tgaactttat gtcgttgacg atcctgagga gccccttttc
     1441 ggaggcgagg tcgagggcgg tcttgttgtg cttgttcatt acggccaggt ctgcgccccg
     1501 gctgatgaga aagtttatga tgccggcgaa tttgccgttt atggcgcaca tgagcggtgt
     1561 atcgccgtcg tggtcccgga aattgaggtc cgcgccgtgc tccaccagga gcctgacgat
     1621 ctccaggttg ccgctcttgc tggcaaaagt gagggccgtg ccgccatccc tgtcgggaac
     1681 gttgacgtcg gccccacggg agacaaggag cctcaggacg cccacgtggt tgttcctgca
     1741 agcatacgtc agcggggtca caccctgcgc gttccgggca ttgacgtccg ccccgttgtc
     1801 gagaagaaac cgcgccgtgt ccgcgaatcc gcgcatcgac gcatacatga gaggagtgtt
     1861 gccgttctga tccgcattgt tgacatcggc gcctttgctg ataaactgga caacactctc
     1921 gaggtcgccc ttttcgatga cggtgatcaa ttcatttgag aatcgtttct ccacctagat
     1981 cattatatac cacctccaaa aaaacggggg gtttttttga ggagctgcca gggaaggtgc
     2041 cggcgcgtct tctgaaaaat ggattgatgc cgggggattt ttcctttata atcactacaa
     2101 tctttatctg gaggacacaa taatggtcaa gtcaaggttc gcgccgagtc caacggggaa
     2161 cctccacata ggcggcgccc ggaccgctct tttcaattat ctttacacga agcatcatgg
     2221 aggggtcttt gtcctgagga tagaggacac ggatgtcgaa cggtcaaagg acgactacgt
     2281 gaaagacata ctcggcggac tgtcctggct cggtatccac tgggacgagg ggccgtacta
     2341 ccagatggac cgtatggacc tctaccggga acacgcctac agactgctgg cggaaggaca
     2401 cgcgtacaag tgttactgca ccgcggagat gctcgaggaa aagagaaaga aggcgctcaa
     2461 ggagggcggc aagccccact atgacaggac atgccgtgac cttctccccg atgacgcctc
     2521 cagtgaaggc aagcctttcg tcatccgctt ccgcagcccc gtaagcggcg aggtgagctt
     2581 caccgacctc atccgcggca cgataacctt caaatgcgag gaactcgacg acctgatcat
     2641 cttccggacc gactcgacgc ccacgtacaa cttcaccgtc gtcgtcgacg acgcgctgat
     2701 gggcatcacc cacatcgtcc gcggcgacga ccacatcaac aacaccccgc ggcagatact
     2761 catctacgag gcgctgaaat accccgtccc gcagttcgcc cacgtgcccc tcattcacgg
     2821 aaaggacagg acccgactgt ccaaacgcca cggcgccacg tctctcctgg aataccgcag
     2881 cgacggcttc ctccccgagg ccctcatgaa ctatctggca cgtctcggct gggcgcacgg
     2941 cgaccaggaa atattcagcc agcaggaact gatcgagaag ttcgaccttc ccaacgtggg
     3001 gaagtcgcct tccgtctttg atatggacaa gctgacctgg ctcaacggcc actacctgaa
     3061 gacccttccc gcggaggata tcgcagggag gctcctgccc tttctccaac gcatggggat
     3121 cgaggcctcc ccggacggcc gacttgtcag gatcgtggtc aacctccgcg agcgcgcgcg
     3181 aaccctgaag gagatggcgc agatggcgga gtactttttc cgcgacgact ttgccttcga
     3241 cgagaccgcc cgcgtgaagt tcctcaccgc cgcgacgaga cccattctcg tcgatttcgc
     3301 cgaagggctc aaggccatcg cctccacgga tgaagaggag agcctcaagg cgctcatcga
     3361 gggattgacg aaaaagtaca atgccaagcc cgtcgggatc atccaaccgg tcagagtggc
     3421 gctttgcggc aggaccgtaa gccccggcat attcgaggtc atcgccatcc tgggcaaaga
     3481 ggccgtggag aaaagattga cgcgggccgt cgaatcgatc cagtgaacca atgaaactgg
     3541 ccagactcga aatcttcggt ttcaaatcct ttcttaaccg caccgtcttc cagttcggcg
     3601 aaggcatcac gtccatcgtc gggcccaacg gctgcggaaa gagcaatata gtggacgcca
     3661 tagtctgggt ccttggcgag aggggcacaa agagcctgag ggtcaaggac atgggcgacg
     3721 tcatctttca cggcagcaac gggaagagac ccgtcaacat cgccgaggtg accctggatc
     3781 tcacggaggg agacagggag ttcgtcgtca aaagacggat ataccgggac ggcaccaacg
     3841 aatattccct caacggcaag atcgtccgct tgaaggacgt gcaggacgcc ttcctgggaa
     3901 cgggcctggg cacgaattcc tacgccatca tcgagcaggg gcgcatcgag gccctgatca
     3961 gcatgaaacc cgccgagcgg cgcgtcgtta tcgaagaggc gagcggcata acgcggttcg
     4021 aggagaagaa gcgggacgcc gtgaaccgca tggaagaaac gacgtcgaac ctggaacgca
     4081 tcgaggacat ataccgtgag gtcaccgctt cgttcggcaa ggcggagcag gagtgggaac
     4141 gctggaaggc ataccaggaa ctggccgaca ggctccacga gatagacagg cagctccttc
     4201 ttgacggcta cgcgaagctc cgaaagcgca tgacaaaggt gcgcgagcgg caggcagacc
     4261 tcgaggaaga gatactccag aaggaagcgg acaggtcgac agtcagagag gagttcgaag
     4321 cgaaggagag cgagttcgcc ctcaccgaca acatcatccg ccagcttgag gtcgacctga
     4381 agagcaagga aaaggacatg gagagccgcc ttctcgagat agagtacctg ggcgaagagg
     4441 tgaaacgtct cgaggcgtcc cgcaaaggtc tcgcggaaga ggagtcctcc atcgccgcgg
     4501 agacggcgcg ccatgagaaa gagatcgatg ccctcaagga aagaagcggg gccctggcgg
     4561 ccgagctgga agaggaaaag ggcaaagagg cgcgcacccg ggatgtagcg cagggcttca
     4621 agacaaatat cgaggagcac gagaagatcc tggaagagtc ccgtactaag ctcttcgtct
     4681 ccatgagcgc cctcacggag actcgcaaca ggatagcaca gatcgagcgg caggccgagg
     4741 agaggcggaa gcgtatggaa cgccaggcag ccgaggtgaa ggaactcgaa atgctcctcg
     4801 catccctcga cgcgaagcgt aaggcccttc acgacacgct gcaggaggcg ctcagagaaa
     4861 aagaatccct gtccgcaagg gaatcggccg ccgcgggcga gcgcgagggc atcctggagg
     4921 aaatggagag gcagaaggct ctcgtcgaga gcgcgcggaa cgagaagagg gtcaaggaag
     4981 agttcctcag acagctcggc ggctacatcg agggccccga ggacgccgta cgcgcggaag
     5041 accgcctcgc caacctgatc aggacggaag aatcgaggat caaggcgctt gaaaggttct
     5101 tctcggaaga gctcgaatac gaggtcgtga agagcgattc cgtggaggcc ctgtccttag
     5161 ccgtgcggga gcgggaaggc aaccgtgtgt tctttcctcc gaaagggata ttcaggaagg
     5221 ccggggacga tgtcgagatg gaggtcgcct ggatagcgga tatcggagag gctctctcgc
     5281 gggtcgctgc cggcgaagaa gggatattca tcaatgacga tgtctatgtc gactcccgcg
     5341 gcatcatcct taccggaggg gcgtcgaaga agatcgacgt gaggcagttc agggagaaac
     5401 tgaagctcga gaaggaactg cagaccctgt cagccgatgt ggaacgccac ggcagtgccc
     5461 ttgcggcctc aaggcctcgt ctcgaggcct gtgacgcaaa gtgccgttcc gcccgcgcgg
     5521 cccgtgaggc acaggaagga aaggcaaggg cgctcgagaa ggaccttctc atggcggaag
     5581 cccagtggaa aagcgccgcc gacaggctca cggcccttca agcagcaaca gagtctcccg
     5641 acgatgccgg ccccgaggtg ctggaatcgc ttgtcgcgga tcgcggccgc cgggaagagg
     5701 aaaaggccgc gatagaggcc tccatggcac gattgaggga aacgctggca ggactgaaga
     5761 aggagcatga ggcggcgcag tcggcatggc acgccgtcac cattgagata gaaagaaaga
     5821 tgaacatgct caagtccata agcgacaacc gggagcgctc cgaggcagcc atacggtcgc
     5881 ttgcagacaa gacacagggg ctcaaggaaa aatacgcggc gatcgacagg gacatcgagg
     5941 gacgcacgca gaagatacgg tatctggagc ggtcatacga ggaactccag gcggacctgc
     6001 agaaacacgt cgcgagattc gaagagctca agacgatgct cggcaacctt catgcccaga
     6061 aacagacgct ccaggaatcc atcgacaccc ttggcaagga gatcgaacgg gtccgggcca
     6121 ggaaggagaa cgccgaaaaa gacctcgccg tcctcgagga gaaacgcctc accatcgtcg
     6181 agcacctcgt cggcacatac ggcatcgacc agccggacga aatgtcggtg cagctgccgg
     6241 cagatataga agaggagcgc gaatcgctca ccgcgaggat agccgaaatg ggtgagatca
     6301 acttcagggc cgagaaggaa taccttgaac tgaaagagag gatatccttc ctggacaagc
     6361 agaaggaaga cctcctcacc gcgatggaat ccctgaggaa gacgataacg aagatcgacc
     6421 agtttacgaa ggagctcttc acggaaacct tcgacaaggt gggcagcgcc ttctcgcgtt
     6481 tcaccgacct tctcttcaag ggcggcaagg gccacctcgc gatcaacgcc gagcagggcg
     6541 gcgtcgatct cttcgtccag cctcccggga aaaaggtgac caggatggag cttctctccg
     6601 gcggcgagaa ggccctcata tcgctggcgt tcctccttgc cctcatggac acgaggccaa
     6661 gccccttcgc gctgatggac gagatagacg cgccgctcga cgacgcgaac ctcgcgggcc
     6721 ttctcgacat catacggggg atgagcctca agtcccagat cgtcttcatc acccacaaca
     6781 ggataaccat ggagtattcg gacatgatat acggtatcac catggaagag cagggcatat
     6841 ccaaaacggt gtccgtaagg ctctagggcg gcaaagccct atccccttgc aaatgctttt
     6901 ttgtacaatg tcgccacatc ccgttgatat actgtatact tccttttctg gtcgcttttg
     6961 acgcgcgtcg gggaagggat ttttttcttg acatttatcc ttagaacact atgatttatt
     7021 attataaagg ggaagggggc gtttggaata tcaggtctat cactatatcc ataccgttgt
     7081 tgcgacaaca acggtttcat tttttcacgt gtcctctttt tataatttgc tgaaaggagg
     7141 tgggacttac cttcagtagg ggaaatgtgt ttttaaaaac gcaaaggggg ttattatgca
     7201 gccaacagta ccagcagtac ctcccgtcac acttcccgat gcatcagtat tgatggcatc
     7261 ctggtatgtg tggtgggttc tcatcatcgg actctttctc atgaccttta tcggcacctg
     7321 gctcataaca agggggtgga aggaatgaat ccgataacgt accttgactt tcagaaacca
     7381 tcaacggaaa tggcgatatg gggcagcatc ggcgttgggc tcatgctcct catctccatc
     7441 atcctcggat ttatgagcaa gagaaaggtg gcgaagggat ccttcgaaga atatcttgtc
     7501 ggcaaacggg atatcggtcc ggtcgttacc ggttgcgccc tggcggcatc ctacctgtcc
     7561 ggctgggcgt tcgccggaag cacgggcgtc gtatatgccg tcggtttctc gggcatgtgg
     7621 ttcgcgggca tctggagcct tctcggcctc atcccgtgct gctggctcgc cgccgtcaag
     7681 acggcctcct tttcgaagaa gctcggcgcg gccacgctgc ctgagacgat cggccgaagg
     7741 tttgagagca agatgctcca gaccgtcgtc gccatctgca tgctctactt cctcttcatg
     7801 tattcggtgg gccagctcaa ggcggcaggc ggcgtatggt acgccacgac gggccttccc
     7861 gcggtatggt gtttgctcct gtccatcttc atcgcctggc tctacatggt cctcggcggc
     7921 tatgtcggca cccagtggtc catggccttc cagggcgcct tcctcggctt tgtcggggcg
     7981 ctcctcggca tatgggcgat catctgggcc ggcggcttct atgaaatatc cgcgaagctt
     8041 tacatggaac cgaaggtggg ccccgcgctc atcaagctca tacggcctga acttcccaag
     8101 gtgggcccga cacagctctt ctcgagcatg gtaggcatac tggccacgcc ggtcatcttc
     8161 ttcaccatgg ccatcggctt cccgcataac gtgagccggt tcctcggcat gggaaccctc
     8221 aacaagaagg gttactggac attgatcatc atggtctacc tcatcgccgg cgtccccatc
     8281 atgctcgact gctcgagcaa cggactcgtg gccaggatga tctacggacc gaacctcctc
     8341 aaggtcaaac cctgggcggc ggacctcgcg gcgcctgagc tagcctttgc cgtcggcggt
     8401 gttcccatga tgacacttta tgtcatgggt cttttcgctg cggcactctc cacgctcgcc
     8461 ggcatggtct tcatcatgag cgccaacatc acacgggacg tcatcaaact gtggtggccg
     8521 caggtatcgg acaagtccat gctgtacctg ggctacttcc tcatcgccct cttccttttc
     8581 ctgcccttct actggacgct cgtcaacccg ccgccgctct tgtccatctt catgggcctc
     8641 gcggccatgg gtctaggcgc catcttcgtc ttcgtgaccg ctgcctccta ctactggaag
     8701 ggtgctacga agtggggcgc gctgtgctgc gttctttacg gcacgtttgg gagcatgtac
     8761 ggcggctaca aggtcctctt caagaaagag gtcggcttgg ggaccttcga gtggtggctc
     8821 gttatcggct gcggcatact gtacttcgtc gtcagcgcca tcacgaaaaa gccctcccag
     8881 gaactgctcg acaagctgtt tccggcgaag agctgatatc ggtcgaaaca acatcgagag
     8941 gggctttcac aagcccctct tttctttcaa cctgcaaggc acaggatcgg gacgatcgga
     9001 caaacgaaaa cggaggggag atggaaacat acaccgtcgc gtggctcgct gcaggagtgg
     9061 gactcctggc atatttcatc tacagcaagt tcaagaagaa atagcgacta ccaggacaat
     9121 ccttttgacg cgagatattt ctgcgcttcg ctgttgccca ttcgggccgc cgttctgtaa
     9181 tcgctgtagg ccttctcgct ctccttcatg atctcgcgca cctctccccg gcttgcgtac
     9241 gccgacgagt tcttcgggtc gatggctatg gccttcgaga aatcatcggc ggccctccgg
     9301 taatccttca ggagatagaa gacgacgccc cgattgtagt acgcctcgta atagttgggg
     9361 tctttcttta tggccaggct gagaaggtct atggcctccc tgggatgtcc cgcgtcgatg
     9421 aactggtacg cctgcctgta ataatccctg gccgttgtcg tgttctcctc atcgcttgaa
     9481 caaccgccgc agatgacaag aacacatgtg aggaagactg caaaaaagta tctcatgggg
     9541 cctcgcagga agttaatctg ttaaaggaga ccccatgatt ctaacctgat gctgccccta
     9601 aagtccaaat ctatattcga ccagccgccc cgtccgcgag attcgtcccc gttacggtgt
     9661 tacttgcacc ttcctatctg tcccgattcg cacttcgaac cgtccatcca gaccgcctcg
     9721 gatagcttgg ccccgctgaa atttgtctcc ttgtcagtca acgccccctt tagattcgcc
     9781 cccctgaggt ccgcgtcatt gaatagcgta cccttcaggt tcgcccccga gagatttgcc
     9841 ccgttcagat tcgccccata gaaatcgatc ccttccaagt tcgcctgaga aaggtcacac
     9901 ttgctgcatt tgttcgtcgt gttgagtttc tgcaggtcct gggcattgaa ggccaacgca
     9961 ccagcggggg aacaaagcaa cgaaacagcg gcaaaaaaca ttgcgatcat cactgtcttt
    10021 ctcataaaca ccctcctttg cgaaaataga aaagccgcca ctcgcgcgcg agatggcggc
    10081 ccgaccatcc atataaccct tcaggaatgt catctccttc ctgaacccga gctatacagc
    10141 cgtttatatt tatttatttt acctttgttt ctccccgtgt caaccccttt tgtcacacct
    10201 cgtgtcacat taggcacgtg gtatggacat acttaccacc tctgtgctcc tgaggcaata
    10261 cccctg
//
LOCUS       DAOQTR010000009        10146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_7422_length_10146_cov_4.092954, whole genome shotgun sequence.
ACCESSION   DAOQTR010000009 DAOQTR010000000
VERSION     DAOQTR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7422_length_10146_cov_4.092954"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKN85_00510"
     CDS             <1..411
                     /locus_tag="PKN85_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNT42913.1"
                     /translation="GTTKEEILTELAGQLKAAGLVKDVDSVVRIIMEREALGSTGIGD
                     GVAIPHGKMKGPGGVLVAFGRSRQGVDFDAVDGQPAHIFFLVLAPEDSASLHLKVLSR
                     ISKILRDPSFRKKLLKLPDAHSLYESIVEEDGKL"
     gene            479..1417
                     /gene="hprK"
                     /locus_tag="PKN85_00515"
     CDS             479..1417
                     /gene="hprK"
                     /locus_tag="PKN85_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNT42914.1"
                     /translation="MKGIEVQQLVDDELYGLELEVLAGKKGLARKIYNPRIQKLGLVI
                     TGHMVYLHPHRVQILGNIEISYLRSLSEAESRRIIRELCKNNVVCFIVSRNLKVPEFF
                     VVSAEEKGIPLLRTRLVTSIFIERITKLLEEKLAPSTTVHGVLMDILGVGALIMGRPG
                     IGKSENALELIMRGHRLVVDDAVHVKKIGAIDLYGEAPEMIKSLLEIRGVGIVDIRHL
                     FGVSAVLDKKRIDLVIELFDWDTSLECERVGLREEKFKVLSVDLPFVKIPISSGKNVS
                     AIIELACRNFILRRQGINTAVELEKRMQRSMEGGTE"
     gene            1414..2265
                     /gene="rapZ"
                     /locus_tag="PKN85_00520"
     CDS             1414..2265
                     /gene="rapZ"
                     /locus_tag="PKN85_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNT42915.1"
                     /translation="MKDTLIVSGISGSGKTTFLRALEDAGFFCVDNFPILLLLKFLEL
                     NRDEGEKIARCAFVIDVRLRDFFENGRDIIEKAKKEFGAKVLFLESSDESLIRRYRET
                     RRSHPLFDSSNVKDALTQERKLMAWLRNMADQVIDTTHLTPHELRRLALERYGAHEQR
                     MKINIMSFGYAFGLPLEADIVLDVRFLPNPFFVEELRDQTGLDEEVREFIRSQDVFRK
                     YFLFLSEFLVYLIPLFEKEGKSYLTVCFGCTGGKHRSVFIAGELAEQLNAMGYSVSTV
                     HRDIEKR"
     gene            2275..2685
                     /locus_tag="PKN85_00525"
     CDS             2275..2685
                     /locus_tag="PKN85_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNT42916.1"
                     /translation="MIGLVVVAHFNLAREMVAATELIVGEQEQFIAVGIFPNERVEET
                     KEKIVSALKDSDTGDGVIILTDMFGGTPSNISLSFLEEGRVEVVAGVNLPMLIKLVTY
                     REGKTLDDLAAFITQYGQKNIYLATDVLKARKKD"
     gene            2692..2958
                     /locus_tag="PKN85_00530"
     CDS             2692..2958
                     /locus_tag="PKN85_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNT42917.1"
                     /translation="MLEGIFTIRNRLGLHARAAAMFVKAAAKFNSIVMVEKDGMEVNG
                     KSIMGLLMLACPLGSKIVLKAEGEDEEKAFRELGGLIDDGFNEE"
     gene            2955..4739
                     /gene="ptsP"
                     /locus_tag="PKN85_00535"
     CDS             2955..4739
                     /gene="ptsP"
                     /locus_tag="PKN85_00535"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNT42918.1"
                     /translation="MKEEILVNQTLKGTGVSSGITMGRVRLIDRDKVSITKRSISPRQ
                     AENEVGRIRNAVQGAIDQLSHIKDSIPDDDVRKHAFIIDAHILILQDPFFVDSVIDVI
                     RSEHINAEWALDIVVSRFLASFDKVEDPYLRERGQDIRYIYERLARILVVGEKPRISS
                     ETVKGKSIIIAHDLSPADTIQLNLNRVSGFATDVGGRTSHTSIVARALEIPAVVGVGN
                     VTMLVRNNDLVIIDGDEGVVIINPVKEVQKEYAAKQIHLRNQKKEHLRVARLKAETKD
                     GFRMRVGANIELLAEMDIVEKYGAVGIGLYRTEYIYLSRKHDLPSEMDHYHIYRKLAE
                     SKTLQYITIRTLDIGGDKFVSEIEMPKEINPAMGLRAIRVCIKEVPLFKAQLKGILRA
                     SQYGPLRILIPMVSGIEEVRKAKAIIAECMDELSHKKIAYNKDIKTGIMIEVPSACMI
                     SDMLAAEVDFFSIGTNDLIQYALAIDRVNEYVSYLYEPLHPAVLKMIKKAVDDAHAHK
                     ITIALCGEMAGEPRYVPVLLGLGLDEISMNPYAIPRAKKAIRGLDHGYCRELLEEIMK
                     KDSPAEAELLLKNEMARLFPGDFPKVRG"
     gene            4791..5948
                     /gene="metK"
                     /locus_tag="PKN85_00540"
     CDS             4791..5948
                     /gene="metK"
                     /locus_tag="PKN85_00540"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNT42919.1"
                     /translation="MSRFLFTSESVAEGHPDKVSDQISDAVLDAIIKDDPGARVACET
                     YVTTGLALVGGEITTSSYVNVPEIVRETVREIGYNDSSMGFDWETCAVLTAIDEQSPD
                     IAMGVNETGDHEQGAGDQGLMFGYACNETAEFMPMSIMYAHKLIRRLADVRKDGTLPF
                     LRPDGKSQVTIEYIDRQPVRVDAVVIAAQHNPDVTIEKIREGVTEEVIKKIIPLELMD
                     DNTKIFINSTGRFVVGGPKGDCGVTGRKIIVDTYGGVGSHGGGAFSGKDPSKVDRSAS
                     YMARYIAKNLVAAGLADRLELQIAYTIGVAQPVSVMIDTQGTSKISPDKIAQIVNDLF
                     DMRPRKIIEKLDLLRPIYKKTACGGHFGRNEAEFTWEKLDMVEEIRKAAGI"
     gene            5985..7310
                     /gene="ahcY"
                     /locus_tag="PKN85_00545"
     CDS             5985..7310
                     /gene="ahcY"
                     /locus_tag="PKN85_00545"
                     /inference="COORDINATES: protein motif:HMM:NF017071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNT42920.1"
                     /translation="MDYDVKDLKLADAGLEKIEWAGRRMPVLGQIAQRFAKKKPLKGV
                     RIACCLHVTSETGNLMKTLKAGGADVVVCASNPLSTQDDVAAAIVKHFKIPAFCIKGE
                     TDRQYYDHIMKALAFMPNITMDDGADLVGALHMIAFDRTEGLHDIIKKWYKKLTKKEK
                     QALIGDVVGSMEETTTGVIRLKSMEKEGVLQFPVVAVNDALTKHMFDNRYGTGQSTID
                     GILRATNVLFAGATFVLAGYGWCGKGVTMRAKGLGARVIVAEVDPLRALEAHMDGFEV
                     MEMEKAAAIGDIFVTTTGDIHVIRKEHFEKMKDGAMVCNSGHFNVELDLDALEKMKKS
                     KRRIRQFIDEYTLKNGKKIFVLGEGRLVNLACAEGHPSDVMDMSFANQALCSEYMWKN
                     AKKLEKKVYSVPKDIDEQVALLKLASAGITIDTLTDEQRRYLASWEMGT"
     gene            7292..8224
                     /locus_tag="PKN85_00550"
     CDS             7292..8224
                     /locus_tag="PKN85_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT42921.1"
                     /translation="MGDGNMIRLLVAGTVAYDSIETPFGKADNVLGGSALHFTNAASF
                     FTDVGIVATVGSDFDMDGISFLKERSVDMSGIEVDHGKTFRWEGKYGYDLNQAITLKT
                     ELNVIETFKPKVPEEYRNAEFLFLANIDPELQLHVIEQVTKPVALVLDTMNFWIENKY
                     EALMKVIGRVDMLVINEAELREITEEHNLLKGARKLIDLGPVCIVVKRGEYGVFMMNR
                     DEVFLAPAYPLEDVFDPTGAGDTFAGGFMGYLANVGEATSETVKQAIIMGTVMASFNV
                     EDFSINRIKRLTMPEIKQRYHALKGCMRFGDISL"
     gene            8896..9816
                     /locus_tag="PKN85_00555"
     CDS             8896..9816
                     /locus_tag="PKN85_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNT42922.1"
                     /translation="MQGIGSQPLAAMSTGDEVLVVHLGGLGDVCLSESVFLSLRGHFG
                     DRLVGLGNRRFFDLFGGYFLRTHGVESRHWLYLFSEKLTGPRWGRIAFIGKDRRGSIR
                     SRWKAHSVEEPLFIDMYPEGAFGSPSPGDGTAAAGETVHVEDYQLRQLSAVGIEPARK
                     EIVPKEARRVILYAEQGFSKEKWPVENFVVLREVLEEAGIDVRLMRPPELSLSTRGSI
                     FFEDLADVKDFFGIGGVFVSNDSGMAHLAGVCGLTTMTIFTAFDPAVWHPRGRNISLR
                     TGVDRTDVESLAGMIIGIMERWDGKGEGLS"
     gene            complement(9843..>10146)
                     /locus_tag="PKN85_00560"
     CDS             complement(9843..>10146)
                     /locus_tag="PKN85_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT42923.1"
                     /translation="RYYLARAYEGMGRTDLAATTYRSLIPYAPSYPEIYYRLGMIEGR
                     TGNEAAGHANLGRYYMYKGNYMLAKTNFEKAISRYGINSRESTELMRLLDSLEEKK"
BASE COUNT         2258 a         2946 c         3060 g         1882 t
ORIGIN      
        1 ggcaccacca aggaggagat actcaccgag cttgcggggc agctcaaggc tgcgggcctt
       61 gtaaaggacg ttgactccgt cgtgaggatc atcatggaac gggaggccct ggggagcacg
      121 ggcataggcg acggcgtcgc cattccccat ggcaagatga agggtcccgg cggggtcctc
      181 gtcgcattcg gccggtcacg tcagggtgtc gatttcgatg ccgtcgacgg gcagccggca
      241 cacatcttct ttctcgtcct cgcccccgag gattcggcaa gcctgcattt gaaggtcctg
      301 tcgcggatat cgaagatcct tcgcgaccct tcgttcagaa agaagctgct gaagctcccc
      361 gatgcccatt ccctgtatga gagcatcgtg gaagaggacg gaaagctgta agggcccgcc
      421 tcgagggacg ggccgggaag catgcctccc tccgggggag gacctcgcgg aaaggcacat
      481 gaagggaata gaggttcaac agcttgtaga tgacgagcta tacggattgg aacttgaggt
      541 tctggccggc aaaaaggggc tcgcccgtaa gatctacaac ccgaggatcc agaagctcgg
      601 cctcgtcata acgggccata tggtctatct ccatccccac agggtccaga tactgggcaa
      661 catcgagata tcctacctga ggtcgctgtc ggaggccgag agccggcgca tcatcaggga
      721 gctctgcaag aacaacgtgg tgtgcttcat cgtctcccgg aacctgaagg tccccgagtt
      781 cttcgtcgtc tcagcggaag agaagggcat cccccttctc cggacaaggc tcgtcacgtc
      841 catattcata gagaggataa cgaagctcct ggaggagaag cttgccccgt cgaccaccgt
      901 ccacggcgtc ctcatggaca ttctgggcgt cggcgccctt atcatgggaa ggccgggcat
      961 cggcaagagc gagaacgccc tcgagctcat catgcggggc catcgccttg tcgtcgatga
     1021 tgccgtgcac gtcaagaaga tcggggccat cgacctctac ggggaggcgc cggagatgat
     1081 aaagagcctg ctggagatac ggggcgtggg catcgtcgat atccggcatc ttttcggcgt
     1141 gtccgccgtc ctcgacaaga agcggataga cctcgtcatc gagctcttcg actgggacac
     1201 ctccctcgag tgcgagcggg tgggattgag ggaagagaag ttcaaggtgc tcagcgtcga
     1261 cctccccttc gtcaagattc cgatcagctc gggcaagaac gtctcggcga tcatcgaact
     1321 ggcatgccgc aatttcatcc tccgcaggca gggcatcaac accgccgtgg agctggagaa
     1381 gaggatgcag agatcaatgg agggagggac ggaatgaagg acaccctcat cgtcagcggc
     1441 atatccggct cggggaagac gacgttcctc agggccctcg aggacgcggg gttcttttgt
     1501 gtcgacaact ttcccatcct tctgctcctc aagttcctgg aactcaaccg cgatgagggg
     1561 gagaagatcg cgcggtgcgc cttcgtcatc gacgtgcgct tgagggactt cttcgagaac
     1621 ggccgggata tcatagaaaa ggccaagaaa gagttcggcg cgaaggtcct cttcctggaa
     1681 agctccgacg agagcctcat tcgtagatac cgggaaacga gacggtccca tcccctcttc
     1741 gactcgtcca acgtcaagga cgcgctgacc caggagcgca agctgatggc ctggctcagg
     1801 aacatggccg atcaggtcat agacacgacc catcttaccc cccacgaact gcgccgcctg
     1861 gcactcgaga gatacggcgc ccacgagcag cggatgaaga tcaacatcat gtccttcggg
     1921 tacgccttcg gtcttccact cgaggccgac atcgtcctcg acgtgaggtt cctgccgaac
     1981 cccttctttg tcgaagagct ccgggaccag accgggctcg atgaagaggt cagggaattc
     2041 atcagatccc aggatgtgtt caggaaatat ttcctttttc tttcggagtt tttggtgtac
     2101 cttataccgt tattcgagaa agaagggaaa agttatctca ctgtctgctt cggctgcacg
     2161 ggcggaaaac accggtcggt cttcatcgcg ggcgaactcg ccgagcagct caatgccatg
     2221 ggctacagcg tatccaccgt ccatcgggat atagagaaga ggtaaggagg cgaaatgatt
     2281 ggtctcgtag tggtggcaca cttcaatctc gcgcgggaga tggttgccgc caccgaactg
     2341 atcgtgggtg aacaggaaca gttcatcgcc gtcggtatat ttcccaacga acgggtggaa
     2401 gagaccaagg agaagatcgt ttcggccttg aaggactcgg acacgggcga cggggtcatc
     2461 atcctgactg atatgttcgg cggcacccct tccaacatca gcctttcctt cctggaagag
     2521 ggccgggtcg aggtggtggc cggcgtcaac ctccccatgc tcatcaagct cgtgacatac
     2581 cgggagggga agacgctcga cgatctggcc gctttcatca cgcagtacgg tcagaagaac
     2641 atatatctcg ccacggatgt cctgaaggcc agaaagaagg attagggagc gatgctggaa
     2701 gggatattta cgataaggaa caggcttggc ctccatgccc gggccgcggc gatgttcgtc
     2761 aaggcggcgg cgaagttcaa ctccatcgtg atggtcgaaa aggacgggat ggaggtgaac
     2821 ggcaagagca tcatgggcct tctgatgctc gcctgtcccc tgggaagcaa gatcgtcctc
     2881 aaggcggaag gggaggacga agaaaaggcc tttcgtgaac tgggaggcct catagacgac
     2941 ggattcaacg aagaatgaag gaagagattc ttgtcaatca gaccctgaag gggaccggcg
     3001 tttcctcggg aataaccatg gggcgggtgc gcctcatcga cagggacaag gtgtcgataa
     3061 cgaagcgctc catatccccg cgccaggcgg agaacgaagt gggccgcatc aggaacgcgg
     3121 tccagggcgc gatagaccag ctcagccaca tcaaggattc gatccccgac gatgacgtga
     3181 gaaagcacgc cttcatcatc gacgcccaca tactcatcct tcaggacccc ttcttcgtcg
     3241 attccgtcat cgacgtgatc cgcagcgagc acataaacgc ggaatgggcc ctcgacatcg
     3301 tggtctcccg gttcctcgcc agcttcgaca aggtggagga cccctacctc agggagaggg
     3361 gccaggacat caggtacatc tacgagaggc tcgcgaggat cctcgtcgtc ggtgagaaac
     3421 cgcgtataag ctcggagacg gtgaagggca agtccatcat catcgcccac gacctgtcgc
     3481 ctgccgacac catccagctc aaccttaacc gcgtctccgg gttcgcgacc gacgtgggcg
     3541 gcaggacctc ccacacgtcc atcgtggccc gggccctcga gatacctgcc gtggtcggcg
     3601 tgggcaacgt gacaatgctc gtcaggaaca acgacctcgt catcatagac ggcgacgagg
     3661 gcgtcgtcat catcaacccc gtcaaggagg tccagaaaga atacgccgcg aagcagatcc
     3721 acctcaggaa ccagaagaaa gagcacctca gggtggcacg gctcaaggcc gagacgaagg
     3781 acggcttccg gatgagggta ggcgccaata tcgagcttct cgcggagatg gacatcgtcg
     3841 agaagtacgg ggccgtcggc ataggcctct acaggaccga gtacatctac ctttcgcgaa
     3901 agcacgacct tccctcggag atggaccact accacatcta tcgcaagttg gcggagagca
     3961 aaacactcca gtacatcacc atccggaccc tcgacatcgg cggcgacaag ttcgtctccg
     4021 agatcgagat gcccaaggag ataaaccccg cgatgggcct gagggccatt cgggtctgca
     4081 tcaaggaggt cccccttttc aaggcgcagc tgaaggggat cctccgggcc agccagtacg
     4141 ggccccttcg catcctcatc cccatggtct cgggcatcga ggaggtgcga aaagcgaagg
     4201 ccatcatcgc cgagtgcatg gacgagctct cgcacaaaaa gatcgcctat aacaaggaca
     4261 taaagacggg gatcatgata gaggtcccct cggcgtgcat gataagcgac atgctcgccg
     4321 cggaggtcga cttcttcagc atcgggacga acgatctcat acagtacgcc ctcgccatcg
     4381 acagggtcaa cgaatacgtc tcgtacctgt atgaaccgct ccatcccgcc gtcctgaaga
     4441 tgatcaagaa ggccgtcgac gatgcccatg ctcacaagat caccatcgcg ctgtgcggtg
     4501 agatggcggg ggaaccacgg tatgtcccgg tgcttttggg cctcggcctc gacgagataa
     4561 gcatgaaccc ctatgccata ccgcgggcga aaaaggccat tcgcggactc gatcacgggt
     4621 actgccggga actcctcgag gagatcatga agaaggactc gccggcggag gcggaactgc
     4681 ttttgaagaa cgaaatggca cgattgttcc ccggggattt cccgaaggtc cggggttagc
     4741 aggatacact tttaccaaac accgacgtca aggaggatta tacaatggga atgagcaggt
     4801 ttttattcac gtccgaatca gttgctgaag gacatccgga caaggtttcc gaccagatat
     4861 ccgacgccgt gctcgacgct atcatcaagg atgacccggg ggctcgggtt gcctgcgaaa
     4921 cgtatgtaac gacgggcctt gccctcgtgg gcggcgagat aacgacgtcg agctacgtca
     4981 acgtccccga gatcgtccgg gaaacggtga gggagatagg gtacaacgac tcgtcgatgg
     5041 gcttcgactg ggagacctgc gcggtcctca cggccatcga cgaacagtcc cccgacatcg
     5101 ccatgggcgt caacgagacg ggcgaccacg agcagggtgc cggcgaccag ggcctcatgt
     5161 tcgggtatgc ctgcaacgag acggcggagt tcatgcccat gtccatcatg tatgcccaca
     5221 agctcatcag gaggctcgcc gatgtccgca aggacgggac ccttcccttc ctgcggcccg
     5281 acggcaagag ccaggtgacc atagagtata tcgacaggca gcccgtcagg gtggacgccg
     5341 tcgtcatcgc ggcccagcac aaccccgacg tgaccatcga aaagatacgc gaaggcgtga
     5401 cggaagaggt catcaagaag atcatcccac tggagctcat ggacgacaac acgaagatct
     5461 tcatcaactc cacgggaagg ttcgtggtcg gcggtcccaa gggtgactgc ggggtgacgg
     5521 gacgcaagat catcgtcgac acctacggtg gcgtcgggag ccacggcggc ggcgcctttt
     5581 ccggtaagga cccctccaag gtcgaccgca gcgcatccta catggcccgc tacatcgcga
     5641 agaacctggt tgccgccggc ctggccgaca gactcgagct gcagatagcc tacacgatcg
     5701 gcgttgctca gcccgtctcc gtcatgatag acacccaggg cacctcgaag ataagccccg
     5761 acaagatcgc gcagatagtc aacgacctct tcgacatgag gccgaggaag atcatcgaga
     5821 agctcgacct cctgcggccc atctacaaga agaccgcctg cggcggccat ttcggcagga
     5881 acgaggcgga attcacctgg gagaagctcg acatggtgga agagataagg aaggccgcgg
     5941 ggatatagga ctcccgcaac gatatcgatc gaaggagaac gcgtatggat tatgatgtga
     6001 aggacctaaa actggccgac gccgggctgg agaagataga gtgggcggga agaaggatgc
     6061 ccgtcctcgg ccagatcgca cagaggttcg cgaagaagaa acccttgaag ggcgtcagga
     6121 tagcctgctg cctccacgtg acgtcggaaa cggggaacct catgaagacc ctgaaggcag
     6181 gaggcgctga cgtggtggtc tgcgcgtcga accccttgag cacgcaggat gacgtggcgg
     6241 ccgccatcgt gaagcacttc aagatacccg ccttctgcat caagggagag acggaccgcc
     6301 aatactacga ccatatcatg aaggccctgg ccttcatgcc caatatcacc atggatgatg
     6361 gggccgacct cgtcggagcc ctgcacatga tcgccttcga ccgcaccgaa ggcctccatg
     6421 acatcatcaa gaaatggtac aagaagctga ccaagaagga gaaacaggcc cttatcggcg
     6481 atgtcgtggg ctccatggag gaaacaacga cgggcgtcat acgcctcaag agcatggaaa
     6541 aggaaggcgt tctccagttc cccgtcgtgg ccgtgaacga cgcgcttacg aagcacatgt
     6601 tcgacaaccg gtacggcacg ggacagagca ccatcgacgg cattctgcgc gccacgaacg
     6661 tcctgtttgc cggcgccacc ttcgtgcttg ccgggtacgg ctggtgcggc aagggtgtca
     6721 ccatgagggc gaagggcctc ggcgcccgcg ttatcgtcgc cgaggtagac ccccttcggg
     6781 cccttgaagc ccacatggac ggcttcgagg tcatggagat ggagaaggcg gcggcgatcg
     6841 gcgacatctt cgtcaccacc accggcgata tccatgtcat ccgcaaggaa catttcgaga
     6901 agatgaagga cggcgccatg gtctgcaact cggggcactt caacgtggaa ctcgacctcg
     6961 acgccctgga gaagatgaag aagtcgaaaa ggcggatccg ccagttcatc gacgagtata
     7021 ccctcaagaa cggcaagaag atattcgtcc tcggcgaggg caggcttgtc aacctcgcct
     7081 gcgcggaggg gcatccctcg gatgtcatgg acatgagctt cgcgaaccag gcgctctgct
     7141 cggagtacat gtggaaaaac gccaagaagc tggagaagaa ggtctattcc gtgccgaagg
     7201 acattgacga gcaggtcgcc ctgctcaagc tcgcatcggc gggaatcacc atcgacaccc
     7261 tgacggacga gcagaggagg tacctcgcgt cgtgggagat gggaacatga tcaggctcct
     7321 tgtcgcgggg acggtggcct acgactccat agagaccccc ttcgggaagg cggacaatgt
     7381 cctcgggggg tcggccctgc acttcaccaa tgccgccagc ttcttcaccg acgtcggcat
     7441 tgtggccacg gtggggagcg atttcgatat ggatggcata tccttcctca aggaacgcag
     7501 cgtcgacatg tccgggatag aggtggacca tggaaagacc ttccgctggg aaggcaagta
     7561 cggatacgac ctcaatcagg ccataacgct caagacagag ctcaacgtca tagagacctt
     7621 caagccgaag gtccccgagg agtatcggaa cgccgaattc ctcttcctcg cgaacatcga
     7681 ccccgagctc cagctccacg tgatcgaaca ggtcaccaaa cccgtcgccc tcgtcctcga
     7741 caccatgaac ttctggatcg agaacaagta cgaggccctc atgaaggtga taggccgggt
     7801 ggatatgctc gtcatcaacg aggcggagct gagggaaata acagaggagc acaatctcct
     7861 caagggcgcc cggaagctca tcgacctcgg ccccgtgtgc atcgtcgtca agcgcgggga
     7921 gtatggcgtc ttcatgatga accgcgacga ggtcttcctc gcccccgcgt acccgctgga
     7981 ggacgtcttc gaccccaccg gtgccggaga cacctttgcc gggggcttca tgggctacct
     8041 cgccaacgtg ggcgaggcta cctcggaaac ggtgaaacag gcaatcatca tgggaaccgt
     8101 catggcctcc ttcaacgtcg aggacttcag catcaacagg atcaagaggc tcaccatgcc
     8161 cgagatcaaa cagaggtatc acgccctgaa gggctgcatg cggttcggag acataagtct
     8221 gtaagggcgg cctgaaccgc ttgagccgct tgaatggctt gagggttagg ggcgagagaa
     8281 gaaagtagat catagatccc cttattttgc tttgcgctgt caaggtaaga taaggggttc
     8341 tttttttcac ccataacccg ctcctcgccg caacccctct cgtttgtccg ggtagcgccc
     8401 accccttcac atacgtccta tgggccctat atgtcctatt cgtcctacga tcgtcccatt
     8461 ggtcctatcc tgcccccgcc ctcatctgtc ccctttcctg gcaatccgtt ctttgcagtt
     8521 gacctggctc cgggaaaatg gttattactt agcaaaccga aaaccaggca atgcactctc
     8581 aagcgtatgt ccgcggcggc cgcggaccgg gtgtgcgccc gtgaagaagc gcggcgacac
     8641 cgtgtggtgc cgttccccgc gagattggcc cctatggagg ccgggagttc gtattgttgg
     8701 tggacgcgcg ctgcgactac ccccacgtgc gaagagcacg ttcaagcgcg gggcgggtct
     8761 gactcacccg cctcgcgcga tctttttcct ttatcgaccc tcgcttttcc cgttacaatg
     8821 gtgcacataa tatgaaaacc agggccgcca tcttcgatat gaaccgcagc atcacaggga
     8881 ccgttggtta gcgatatgca gggaataggg tcgcaacccc tcgcggcaat gtcgaccggg
     8941 gacgaggtcc tcgtcgtcca tctcggcggc cttggcgatg tatgtctctc agaatccgtc
     9001 ttcctctccc tgcgcgggca tttcggggac cgcctcgtgg gcctgggcaa cagacgtttt
     9061 ttcgacctct ttggaggcta ctttctcaga acccatgggg tggaatcacg ccactggctc
     9121 tacctcttct ccgagaagct cacggggccg cgctggggtc gcatcgcctt tatcggcaag
     9181 gaccgccggg gttccatccg cagccgctgg aaggctcatt ccgtcgagga gccgctgttc
     9241 atcgacatgt accccgaggg ggcattcggc tcaccttccc cgggcgacgg gactgccgcc
     9301 gccggggaga ccgtgcacgt tgaagactac cagttgcggc agctctctgc cgtggggata
     9361 gagccggcgc ggaaggagat agttccgaaa gaggcgcgac gggtcatcct ctacgccgag
     9421 caagggtttt cgaaggagaa gtggcctgtg gagaacttcg ttgtcctccg ggaggtgctt
     9481 gaggaggccg gcattgacgt gcgtctgatg aggccgcccg agctgtccct ttccacccgg
     9541 gggtccattt tcttcgaaga tctcgccgac gtaaaggact tcttcggcat cggaggcgtc
     9601 ttcgtgtcca acgattccgg catggcccat cttgccggtg tctgcggcct gaccacgatg
     9661 accatcttca ccgccttcga ccctgccgtg tggcatccca ggggaaggaa catctcgctg
     9721 agaaccggcg tcgacaggac cgatgtcgag tcgctcgccg ggatgatcat cgggataatg
     9781 gaacgatggg atggaaaggg agagggtctt tcctgaatcc tgcccccgga ccccttattc
     9841 tactatttct tctcttccag gctgtcgagg aggcgcatga gttccgtgct ctcccgtgaa
     9901 ttgatgccgt agcgggagat ggccttttcg aaattcgttt tcgccagcat gtagttgccc
     9961 ttatacatgt agtaccggcc caggttggcg tggcccgcgg cctcgttgcc cgtcctgccc
    10021 tcgatcatgc cgagacggta gtatatctcc ggataggatg gggcgtaggg gatgagggac
    10081 cggtaggtgg ttgcggcaag atcggtcctg cccatgccct cgtatgccct ggcgagatag
    10141 tagcgg
//
LOCUS       DAOQTR010000010         9153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_8442_length_9153_cov_3.123763, whole genome shotgun sequence.
ACCESSION   DAOQTR010000010 DAOQTR010000000
VERSION     DAOQTR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8442_length_9153_cov_3.123763"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="PKN85_00565"
     CDS             <1..1589
                     /locus_tag="PKN85_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNT42924.1"
                     /translation="TQEIRLYYEFYEVDTDRYHLEGEGYRQVMLSARELVGQLPEGAR
                     TWINERLQFTHGYGVAMAFVSEQVEGGLPRYVVKDLPPASPYLQIDRPAIYYGEMMPG
                     YRIVNTQIKEFDYPKGDDNVYTNYEGTGGVQLDTFWRRLLFAWDLFDINILISSYITP
                     ESRIQIWRRVQERMSRIAPFLRLDKDPYIVIVDGKLYWIQDAYTVSSHFPYSEPYKGG
                     INYIRNSVKIIVDAYNGTVSFYVMDPHDPVLAVYRRLFPNVFQGIDRLPVALKDHLRY
                     PQDLFEAQVDKFQTYHMTDPQVFYNREDLWTLPQEKYAGTPIPVEPYYILMRLPGERT
                     LQYLLMTPLTPRNKDNMIAWMAARCDFPEYGQLLAYQLPKERLIYGPLQIEAMIDQDD
                     VISQQLSLWDQRGSRVIRGNLLVIPVDQSFIYVEPVFLVAEKGDIPQLKRVIIVYGEK
                     VVMEPTIGEAIEAVFGVVRRPAGEVPAAPETGVIADSARRELERAQNALGRGNWAEFG
                     RAMDRLKEVLARPQDRSKSK"
     gene            1617..4007
                     /gene="ppsA"
                     /locus_tag="PKN85_00570"
     CDS             1617..4007
                     /gene="ppsA"
                     /locus_tag="PKN85_00570"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNT42925.1"
                     /translation="MTNRAKYIRWFETLGSKDVHLVGGKNASLGEMIHSLKKEGIRVP
                     DGFATTSDAYWSLLDAAHLVEKVEELLDEFERDERSLEKVGRAIRGLFLKAEFPEALS
                     GAIGKAYAELCQRYRTDDVDVAVRSSATAEDLPEASFAGQQESFLNISGEEELLEACR
                     RCYASLFTDRAIAYRKEKGFDHMKVALSVGVQKMVRSDEAGAGVMFSIDTETGFPDAV
                     VINAAWGLGENVVQGSVTPDEYTVFKPLLDKKGLSPIIDKTLGSKEKKMIYARGGTRA
                     TRNIDTSKEERRSFVLSDKEILGLARWAYIIEKHYRKPMDIEWAKDGETGELFIVQAR
                     PETVQSQKEASSLKTYKLKKRSKRLVTGLSIGEAIAAGEACKIKSAENVGRFKEGSVL
                     VTEMTDPDWVPIMKRAKGIVTDYGGRTCHAAIVSRELGIPAIVGTGNGSEVLKDGQEV
                     TVSCAEGDQGYVYEGILEYEETEVDLKKIPKIRTRIMMNIASPAAAFRWWRLPCEGIG
                     LARMEFIINNIIKIHPMALVRYDKLEDKEARQRIGELTIGYEDKTEYFVDHLSLGIAK
                     IAASRYPHQVIVRMSDFKTNEYANLIGGKEFEPREENPMLGFRGASRYYSERYREGFA
                     LECKAVKRVREKMGLRNVTIMIPFCRTLEEADRVMRVLAKNGLKRGENDLQVYVMCEI
                     PSNVILADQFARRFDGFSIGSNDLTQLVLGVDRDSAELAPLFDERNDAVKKMIQDLIK
                     TAHRESCKVGICGQAPSDYPDFAAFLVKAGIDSISLNPDSIVEVKKRIARVRGKRG"
     gene            4056..5789
                     /gene="polX"
                     /locus_tag="PKN85_00575"
     CDS             4056..5789
                     /gene="polX"
                     /locus_tag="PKN85_00575"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNT42926.1"
                     /translation="MPIHNREIADVFNRVADLLDIEGENQFRIRAYRKAARIVVDLPR
                     NVGDMVREGEDLTRFSGIGKDLAGKIKEIVETGVLGQLKELEKKTPPDLSRLMKMEGL
                     GPKRVQTLHKELGINDLDGLKKAAEENRIRKLEGFGEKTEKLILEALERMKDEGEKER
                     IKLFAAEQIANDYVDYLKKGRGIKDIAVAGSYRRRMETVGDLDILVTCKKGSQVMERF
                     VEYEDVAKVLSKGTTRSSVVLRTGLHVDLRLVPQVSYGAALHYFTGSRDHNIAVRGLG
                     VKLGLKINEYGVFRGKDEERVAGRTEEEVYRQVGLPYIEPELRENRGEIEAARNNKVP
                     ILIRRDDIRGDLHVHTNETDGRQTLEEMVQAAREQGYEYLAITDHSKRVTMAKGLDAR
                     RLAKQIDAIDTLNGKVRGIRILKGIELDILEDGSLDLPDDILKELDLTVCSIHYNQNL
                     SRKRMTERVIRAMDNPFFTIFAHPTGRLIGGRKGYEIDLEAIMKAAGERGCVLELNAH
                     PDRLDLDDHNCRMAKEMGLRVVISTDSHSSSDLDFMRFGVYQARRGWLEKDDVLNTRS
                     FSEIEGLLKRK"
     gene            6096..8789
                     /locus_tag="PKN85_00580"
     CDS             6096..8789
                     /locus_tag="PKN85_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNT42927.1"
                     /translation="MNLPRKPWSREWQEVTKDLEVSAEEGLSTQESRRRRKQFGSNRL
                     RATRRRSGPAILVDQIKNPIVLLLAVAAAVSFSFSRVLEGISILIAVVINCTIGFFTE
                     LKAVRSMEALHRLSRVTAKTLRERRLQDAPSEELVPGDIVMLEAGDLVPADLRLTEAS
                     RLQIDESAITGESVPVTKSVEVLEEQTSMAERANMALKGTAVTAGSGHGVVTATGMNT
                     ELGRVASMVEEAEGETTPLEKKLDRLAYRLLWITLGIGVIVAVTGVAAQKEIFLIIET
                     SIALAVAAIPEGLPIVATIALARGMWRMAKHNALINRLSAVETLGAVSVIFTDKTGTL
                     TENRMQVAKVILASRDGLDTVDVRADSEDASGGLFMKGQPLNISDQPVLRKVFEVGVL
                     CNNAEIDHGDVSKGVGDPMELALLGAGRKVGIDRNELLDSMPEVREESFDQKSMMMAT
                     IHKTDGGFLVAVKGAPEAVLKASRHIPGQGGPGVLEEATRDRWRQENHALAEAGLRVL
                     AVATRESPSTEIDPYEDLTLLGLIGLADPPRHDVKAAVQACRNAGITVIMVTGDQAPT
                     ARYVGQALGLIDGNGSGIIQGKDLGNPGELSDEERGRLLKARIFARVNPGQKLDLITL
                     HQDNSSVVAMTGDGVNDAPALKKADIGIAMGQRGTQVAVEAADMVLQDDAFRTISVAI
                     QQGRAIFDNIRKFILFLLSGNAGEILIVLFAILAGLPLPILPLQILYLNMIGDLFPAL
                     ALGVGKGDASKMERPPRDPEEPLFTTRHWLATGGYGLLIALSVTGSFALALKYFGMEI
                     NSAVTVSFLTLAFARIWHVFNMRDTGCGILRNDVTRNPFVWAALAVCVGMLISAVYLP
                     VVSKALRLVEPGAEGWALIIGGSVVPWLVGQFLKCLRIGKP"
     gene            complement(8867..>9153)
                     /locus_tag="PKN85_00585"
     CDS             complement(8867..>9153)
                     /locus_tag="PKN85_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42928.1"
                     /translation="EEPLIEEAVHEFVSREPARPSPSGLDVPDKTVPGQSGSHAARDT
                     DRTSTASSDRSRLKVLVLKSSFFQPALVRRLVIGAMLIGSALTALYLLLF"
BASE COUNT         2125 a         2362 c         2926 g         1740 t
ORIGIN      
        1 agacccagga gatccgtctt tactacgagt tttacgaggt ggatacggac cgctatcatc
       61 tggaaggcga agggtatcgc caggtgatgt tgtcagctcg cgagctggtc ggccagttgc
      121 ctgaaggggc caggacctgg atcaacgaac ggcttcaatt cacgcacggc tacggtgtgg
      181 caatggcctt tgtttcagag caggtcgaag ggggactgcc ccgctatgtc gtgaaggatc
      241 ttcccccggc ctcgccctat ttacagatag accggccggc gatctactac ggggagatga
      301 tgcccgggta ccgtatcgtc aacacgcaga taaaagagtt cgattacccg aagggagacg
      361 acaacgtcta caccaattac gagggcacgg gaggcgtgca gcttgatact ttttggaggc
      421 ggctcctatt cgcctgggac cttttcgaca taaacatcct gatcagttcc tacatcacgc
      481 cggagagcag gatccagatc tggaggcgcg tgcaggagcg catgtccagg atcgcgccct
      541 ttttgcggct tgacaaggat ccctacatcg tcatcgtcga tggcaagctc tactggatcc
      601 aggacgccta cacggtctcg tcacacttcc cgtattcaga gccctacaaa ggtgggataa
      661 actatatccg caattcagta aagatcattg tcgacgctta caacgggacc gtctcgttct
      721 acgtgatgga ccctcatgat ccggtgctcg cggtgtaccg gcgccttttc ccgaatgtct
      781 ttcagggcat cgacaggctg cccgttgccc tgaaagacca cctgcgctat ccgcaggacc
      841 tcttcgaagc gcaggtggac aagtttcaga cgtatcatat gaccgatcct caggtcttct
      901 acaacaggga ggacctctgg accctcccgc aggaaaagta cgccggaacg ccgatcccgg
      961 tcgagcccta ttatatcctg atgcggcttc ccggggaaag gaccctgcag tatctcctca
     1021 tgaccccgct cacccctcgg aacaaggata acatgatcgc ctggatggcg gcgaggtgtg
     1081 acttccccga gtacggccag ctccttgcct accagttgcc caaggaacgc ctcatatacg
     1141 gaccgcttca gatcgaagcc atgatcgatc aggacgacgt gatctcgcag cagctctctc
     1201 tgtgggacca gcggggctcc cgggtcattc ggggcaacct gctcgtgatc cccgtcgacc
     1261 agtcgttcat ctatgtggaa cctgtattcc tggtggcgga gaagggagac attccgcagc
     1321 tcaaacgggt catcatcgtg tacggggaaa aggttgtcat ggagccaacg atcggtgagg
     1381 cgatcgaggc tgttttcggg gtcgtccgga gacccgcggg agaagtgccc gctgctcccg
     1441 agacgggcgt catcgctgac agcgcaagga gagaactgga gagagcccag aatgcgctcg
     1501 gccgcgggaa ctgggccgag ttcgggcggg ccatggaccg gttaaaggaa gtgctcgccc
     1561 ggccgcagga tagaagtaaa agtaaatagt ggccattaca ggcctgtgga ggtgtcatga
     1621 cgaatcgcgc aaagtacatc cgttggtttg agacactggg ttccaaagat gtgcacctgg
     1681 tcggcggaaa gaacgcctct cttggggaga tgatccattc tctgaaaaag gagggcatcc
     1741 gggtgccgga tgggttcgcc accacgtccg atgcatactg gtcgttgctg gatgccgctc
     1801 accttgtcga gaaggtagag gagttgctcg acgagttcga aagggatgaa agatcgctgg
     1861 aaaaggtggg cagggcgatc cggggactct tcctgaaagc ggagtttccg gaggcgcttt
     1921 ccggggctat cgggaaggcc tacgccgagt tgtgccagag atacaggacg gacgatgtcg
     1981 atgtcgcggt gcgcagcagc gccacagccg aggaccttcc cgaagcgagc ttcgcgggac
     2041 aacaggagag cttcctgaac atttccgggg aagaggaatt gctcgaggcg tgccgcagat
     2101 gctatgcctc gctcttcacg gaccgggcca ttgcctaccg gaaggagaag gggttcgatc
     2161 atatgaaggt ggccctttcc gttggtgtcc agaaaatggt ccgatccgac gaggcaggcg
     2221 ccggcgtgat gttttccatc gacaccgaga cagggttccc cgatgccgtg gttatcaatg
     2281 ccgcctgggg attgggcgag aacgtggttc agggctcggt gacccctgat gaatataccg
     2341 tcttcaaacc gttgctggat aaaaagggcc tttctcccat catcgacaaa acactgggaa
     2401 gcaaggagaa gaagatgatc tacgccagag gggggacacg ggccaccagg aacatcgata
     2461 cttcaaaaga agaacgccgc agtttcgttc tgagcgacaa agagatcctg gggctggccc
     2521 gatgggcata catcatcgag aagcattaca ggaagccaat ggatatagag tgggccaaag
     2581 acggggaaac cggggagctt ttcattgttc aggctcgccc ggagacggtc cagtcccaga
     2641 aggaggccag ctcattaaag acatacaaac tcaagaagag gagcaaaaga ctggtcacgg
     2701 gcctgagcat aggcgaggca attgccgcag gagaagcatg caagatcaaa agcgccgaga
     2761 atgtcgggcg attcaaagag ggttctgtcc tggtcaccga gatgactgac ccggactggg
     2821 tgcccatcat gaaacgggcg aagggaatcg taacagacta tggaggtcgg acctgtcacg
     2881 ccgcgatagt gagccgggaa ctcggtattc cggcgatcgt gggaacaggc aacgggtcgg
     2941 aggtcttaaa ggacggtcag gaggtaacgg tatcgtgcgc ggagggggat caagggtacg
     3001 tctatgaggg catcctggag tatgaggaga cagaggtcga tctcaagaag atccccaaaa
     3061 tacgtacgcg gatcatgatg aatattgcca gtccggcggc cgccttccga tggtggagac
     3121 ttccctgtga ggggatcgga ctagcaagaa tggaattcat catcaacaat atcatcaaga
     3181 tccaccccat ggcgctggtt cgttacgata aattggagga caaagaagca cgtcagcgca
     3241 tcggggagct gaccatcgga tacgaagaca agacagagta ctttgtcgac catctctcgc
     3301 tgggcatcgc gaagatagcg gcatcccggt atccccacca ggtgatcgtc cgaatgagtg
     3361 atttcaagac gaacgaatac gccaacctta tcgggggaaa ggagttcgag cccagggaag
     3421 agaacccgat gctcggattt cgcggcgcct cccggtatta cagtgaacgc tatcgcgagg
     3481 gctttgctct cgaatgcaag gctgtcaaaa gggtcaggga gaagatggga ttgaggaacg
     3541 ttacgatcat gatcccattc tgcaggacgc tggaggaggc ggaccgcgtc atgcgggtcc
     3601 tggcaaagaa tggtctgaaa cgaggcgaga acgatcttca ggtctacgtc atgtgcgaga
     3661 ttccctcgaa tgtcatactg gcagatcagt tcgccagacg gtttgacgga ttctccatcg
     3721 gaagcaacga cctcacgcag ttggtattgg gagttgatcg cgactcggct gaactcgccc
     3781 ccctcttcga tgaaaggaac gacgcggtca aaaaaatgat ccaggacctc atcaaaacag
     3841 cccatcggga aagctgcaag gtcggcattt gcggccaggc gcccagtgat tatcctgact
     3901 tcgcagcttt tcttgtaaag gctggtatcg attctatctc cttgaatccg gacagcatcg
     3961 tcgaggtgaa gaaacggatc gcccgggtcc gggggaagag gggttgaacg aacctcgttg
     4021 cgctggcgat ccactcaaag gctcaggaga gcaagatgcc catacataac agggagatag
     4081 cggacgtttt caaccgcgtg gccgatctac tggatatcga aggagaaaac cagtttcgca
     4141 ttcgggccta tcgaaaagcc gcccgtatcg ttgtggacct gccgagaaat gtcggagaca
     4201 tggtaaggga gggcgaggac ctgacacgat tctcgggcat tggcaaggac ctcgcgggaa
     4261 agatcaaaga gatcgtcgag acgggtgtac ttggccagct gaaggagctc gaaaagaaga
     4321 cgccccccga tctgagccgg ttgatgaaga tggaagggtt gggtccgaaa agggttcaga
     4381 cgctccacaa agaactgggg atcaatgatc tcgacggcct gaaaaaggcg gcggaggaga
     4441 acaggatccg gaaactggaa gggtttggag agaagaccga aaagctgatc ctggaggcct
     4501 tggagcggat gaaggacgaa ggggagaaag agaggatcaa gctctttgcc gccgagcaga
     4561 tcgcgaatga ttatgtggac tatctgaaga aggggagagg gataaaggac attgccgtgg
     4621 cggggagcta ccggcgcagg atggagactg tgggggacct cgacatcctg gtaacgtgca
     4681 agaaagggtc ccaggtaatg gagcgtttcg tggagtacga ggatgtcgcg aaggttttat
     4741 ccaaaggaac cacccggtca tcggtagtgc ttcggacagg gctgcacgtc gacctgcgac
     4801 tcgtgcccca ggtgagctat ggtgcggcgc ttcactactt caccggctcc agggaccaca
     4861 acatcgcggt gcgcggcctc ggcgtgaaac tcgggttgaa gatcaacgaa tacggcgtct
     4921 tcaggggaaa agacgaggag cgggttgccg ggagaacgga ggaggaagtt tacagacagg
     4981 ttggtcttcc ctacatcgaa ccggagctca gggagaaccg gggggagata gaggcggccc
     5041 ggaacaacaa agttcccatt ctcatcaggc gtgacgatat tcggggagac ctccatgtgc
     5101 acacgaatga aacagacggc cgtcagaccc tcgaggaaat ggtgcaggcc gcgcgtgaac
     5161 aagggtatga atatctggcc atcaccgacc attcaaagcg ggtcaccatg gcgaagggac
     5221 tggatgccag gcggctggca aagcagatcg acgcaatcga cacgttgaat ggcaaggtca
     5281 gggggatcag gatcctcaag ggcattgagc tcgatatcct ggaggacggc tccctcgacc
     5341 ttcccgacga catcctcaag gaactcgatc tgacggtctg ttcgatccac tacaatcaga
     5401 acctttcccg aaaacggatg accgagcggg tcatcagggc catggacaat cccttcttca
     5461 cgatcttcgc gcatcccaca ggccgtctca tcggcgggcg gaaaggctac gagatcgatc
     5521 tggaggcaat aatgaaggca gcaggggagc gagggtgtgt gttggagctc aacgcccatc
     5581 ctgaccgcct cgatctggac gatcacaact gccggatggc gaaggagatg gggctcaggg
     5641 tggtgatctc gacagactct cacagctcgt ccgacctcga tttcatgcgc tttggagtgt
     5701 atcaggcccg gaggggatgg cttgagaagg acgacgtgct gaacacgaga tctttttcgg
     5761 agatcgaggg tcttctgaaa agaaaatagg acctcacagg cggcctgcca acagcagact
     5821 gaggaaggtt tgccctacaa cattctcggc ttccccgtcg agccacgctg cataacgaac
     5881 gtgaggtgga acagcaagac cctcgtcccg gtgatagggt tcgaattgaa agggcggggg
     5941 acgtgatccc tgaggtgcct tcgcattaaa ctgagttccg gcctgagcga gctgcaggag
     6001 caggtggtcc acgaggtcac ggagaaagag ttgggagtga gccgacggtt cccgacgggc
     6061 gagacatcat aagcaaaggg ggcgggaaga gacgaatgaa tctgccgcgc aaaccatggt
     6121 cgcgggaatg gcaggaagta acgaaagacc ttgaggtctc cgcggaggag gggctgagca
     6181 cccaggaatc caggagaagg cggaaacaat tcggatcgaa tcggctccgc gcgaccagac
     6241 ggaggtctgg tccggcaatc ctggtagacc agatcaagaa cccaatcgtg ctgttgctgg
     6301 cggtggcggc ggcggtgtcg ttctcgttca gccgcgtgct cgaagggatc tcgattctca
     6361 tcgccgtcgt catcaattgc accatcggat tcttcaccga gttgaaggcg gttcgttcca
     6421 tggaagccct tcatcgcctg agccgggtca ccgcgaaaac actgcgggag cggcggcttc
     6481 aagatgctcc gtcggaagag ctggttccgg gggatatagt tatgctcgag gcgggggatc
     6541 ttgtgccggc ggacctacgc ctcaccgaag catcaaggct tcagattgat gaatcggcga
     6601 ttaccggcga gtccgtaccc gtcacgaagt ccgtggaggt gctcgaggaa cagacgagca
     6661 tggccgagcg cgccaatatg gccctcaaag gcaccgccgt aacagccggg tccggccatg
     6721 gagtggtgac agccacgggg atgaacacag agctcggcag ggtggcctcg atggtcgaag
     6781 aagctgaagg ggagaccacc ccgctggaaa agaaactcga tcgccttgcg tacaggcttc
     6841 tgtggatcac cctggggata ggcgtcatcg tggccgtgac aggagtagcg gcgcagaagg
     6901 agatctttct catcatcgag acttccatcg cgctcgccgt ggccgccata ccggagggtt
     6961 tgcccatcgt ggccaccatc gcgctggcgc gtggaatgtg gcgcatggca aagcacaacg
     7021 cattgataaa tcgcctttcg gctgtcgaga cgctgggcgc cgtcagcgtg atcttcactg
     7081 acaagacggg cacgctcaca gagaaccgga tgcaagtggc aaaggtgatc cttgcgtccc
     7141 gggacgggct ggatacggtg gatgtccggg cggacagcga ggatgcgtcc ggcggcctct
     7201 tcatgaaagg gcagccgttg aacatatccg atcagccggt gcttcgcaag gtgttcgagg
     7261 tgggagtgct ctgcaataat gcggaaatag atcacgggga cgtctcgaag ggtgtcggag
     7321 atccaatgga gttggccctc ctgggcgcgg gccgaaaggt gggaattgac aggaacgagt
     7381 tgctggactc catgcctgaa gtgagagagg agtccttcga tcagaaaagc atgatgatgg
     7441 ctacgatcca caagaccgac gggggttttc ttgtcgccgt gaaaggggct cctgaggctg
     7501 ttttgaaggc ctcccggcac ataccgggcc agggaggccc cggggtgctg gaagaagcga
     7561 cccgtgacag gtggcggcag gaaaaccatg ccctggccga ggctgggtta cgtgttctgg
     7621 ccgtggcgac cagggagtcg ccgtccacgg agatcgatcc ttacgaagac ctgacgcttc
     7681 tcgggctcat cggattggcg gatccgcccc ggcacgatgt caaagcagcc gttcaggcat
     7741 gcaggaacgc gggtatcacg gtgatcatgg tcacgggaga tcaggctccg actgcacggt
     7801 acgtggggca agccctggga ttgatcgatg gcaacggttc ggggataatc caagggaagg
     7861 atctgggaaa ccccggtgag ttgtccgacg aagagcgcgg gcggctgttg aaggctcgca
     7921 tatttgccag ggtgaatccc gggcagaaac tggatctcat cactcttcat caggacaaca
     7981 gctccgtggt cgccatgacc ggcgacggtg taaacgacgc tcccgccctc aagaaggcgg
     8041 atatcgggat tgccatggga cagcggggaa cacaggtcgc ggtcgaagcc gcggacatgg
     8101 tgctgcagga cgacgcgttc cggacgatca gcgtggcgat ccagcaggga agggccattt
     8161 tcgataatat acggaaattt atccttttcc tgttatccgg caatgcgggc gagatcctga
     8221 tagtcctatt cgcgatactg gccggcctgc ccctgcccat tctccctctc cagatcctct
     8281 atctgaacat gatcggggac ttgtttccgg cgctggcgct gggggttggc aagggagacg
     8341 cttcaaagat ggaacgtcct ccgcgggacc ctgaggaacc tctcttcaca acaaggcact
     8401 ggcttgccac gggcggttat ggcctgttga tcgccctatc cgtgacaggc agcttcgcgc
     8461 tggccctgaa gtattttggt atggagataa actcggcggt aacggtgtcg ttcctgacgc
     8521 tcgcgtttgc aaggatctgg catgtcttca acatgaggga cacgggctgt ggcatcctgc
     8581 ggaatgatgt gactcgaaac ccattcgtct gggccgcgct tgccgtgtgt gttggcatgc
     8641 ttatctccgc tgtgtacttg cctgttgttt ccaaagcgct caggttggtc gagcccggcg
     8701 cggagggatg ggccctgata atcggaggga gcgtggttcc ctggctggtg ggacagtttt
     8761 tgaaatgcct gagaatcggg aagccatgaa acttttcaat atcgcgatgt cttcggggca
     8821 cgacgggtca aggccaatga cgatggcgga tgaatacacg ccgtacctag aataggagca
     8881 ggtacagagc ggtcagcgct gatcctatga gcattgcgcc gatcaccagg cgccgcacca
     8941 gagcgggttg aaagaaggat gacttcagca ccaggacctt tagacgggac ctgtcgctcg
     9001 aagcggtcga tgtccggtcc gtgtcccggg cagcgtggct gccgctctgc ccgggaacgg
     9061 tcttgtcggg cacgtccagc ccgctcggtg aaggtcttgc aggctcccgg gacacgaatt
     9121 catggaccgc ctcttcgatc agcggctcct cgg
//
LOCUS       DAOQTR010000011         9111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_8481_length_9111_cov_3.786440, whole genome shotgun sequence.
ACCESSION   DAOQTR010000011 DAOQTR010000000
VERSION     DAOQTR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8481_length_9111_cov_3.786440"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKN85_00590"
     CDS             complement(<1..745)
                     /locus_tag="PKN85_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42929.1"
                     /translation="MDELVRAIDSAQDLEKKVEYYFTLTGDERVSVLEGLGKERNEET
                     GVLLNAIYLQETDKEIRKLIRKTIFRLRSVGVKVEDLRPEGEPVLRKAEESRTNMGFL
                     TNFDHAGSRMVMAAYEVRKNTFVFLNGDLHFREGLRELMSSPVDRRSLNEIVAAYRSG
                     TKEPAFLVEISPSYAAFIVEEGARLSGRFTDAIQSLASFVAQLKDTVHKPDDIHSLPV
                     ADDVAPQPLHEVLRHEIFTPFSLSWEGIEG"
     gene            complement(773..976)
                     /locus_tag="PKN85_00595"
     CDS             complement(773..976)
                     /locus_tag="PKN85_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNT42930.1"
                     /translation="MAEAKLKIDGMSCQHCVMAVKKALGAVAGVDSSDVDIGSAVVRY
                     DEGKASQKDLEAAVEKAGFSVKH"
     gene            complement(1110..2720)
                     /locus_tag="PKN85_00600"
     CDS             complement(1110..2720)
                     /locus_tag="PKN85_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNT42931.1"
                     /translation="MIHYVFLFLAGLALFLLGMLKLSEQMQRVFSSRIREYIRFSVRK
                     PFYGLITGFVTTIFFQSSSATTLLTMGLVSAGLVSFYHSLGIILGADIGTTLTVQLVV
                     WKVTDISPLFLFSGIVLYFLGRDRVRTVGEALLYFGTIFYGLSLIGDAAAPLKDSPVF
                     GRYFLSAQNPLLALLLGLVFTGIVQASAIPISMLVMLGMQGLMSIDNAVPIVIGANVG
                     TTVTALIGSLASDVNGRRTALAHLIFKCSGALVCMLFLSPFVTVLKAMSSSIAQQVAL
                     CHFLFNIVIVFIFMFLLTPFSNAIRRIIPGEHAVIAMWPEYLNTKHLGNPDMALVCVR
                     NELKRQIALAQRMLLESLELFVAYRRNKRQNVMYMELVMDNLQTEITTYLWGISCGAL
                     SQPLTKKLFAFSSFAYDIERMGDRSMNLGELAEQKFRRKAHFTPAANEELRDIGTLVL
                     DNLADAGRLIEKKDIALVRLIVEREHRIDVKIRNATGKHLERFYKKLCLAEAGPIYVD
                     VLVNLEHISNHCLVIAQLTNELEEEETV"
     gene            complement(2727..3056)
                     /gene="metG"
                     /locus_tag="PKN85_00605"
     CDS             complement(2727..3056)
                     /gene="metG"
                     /locus_tag="PKN85_00605"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNT42932.1"
                     /translation="MENITFDDFIKIDLRVAEIRACEDIEGADKLYKLTIDMGEERLI
                     VAGIKQYYTKEELVGKKIAVVANLEPRKLRGIMSHGMLLAASSEDKSSVVLLTPDKAI
                     PNGSKIS"
     gene            complement(3109..3735)
                     /locus_tag="PKN85_00610"
     CDS             complement(3109..3735)
                     /locus_tag="PKN85_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNT42933.1"
                     /translation="MGISFNPSKVVAVGLNYRDHARELKMDLPDYPLIFMKPSTSVVG
                     DGDPIVFPAQTQELHYEGELGIVIGKRARNVPVGEARDFIAGYTCANDVTARDLQRMD
                     GQWTRAKSFDTFCPLGPRIVRDIDPTSLEIVTRVNGGVKQRSTTANMVFDVYDLVSFI
                     SGIMTLLPGDVIITGTPPGVGSLLPGDTVEVEIEGIGTLTNTVTAAVP"
     gene            3871..5334
                     /locus_tag="PKN85_00615"
     CDS             3871..5334
                     /locus_tag="PKN85_00615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNT42934.1"
                     /translation="MSITSFRTLFYHVLLLAVVALAAAGCAINPVTGEQELMLVSERQ
                     EIQLGNELYPNALWSGEGGGGEFRDPALKSYLSSLVVNIHRVSHRPNLPVDFAVQNTS
                     VPNAWAIPGHVVMTRGLLAALDNEAEFVYVMGHEMGHVSARHSARQMTYGMLGQFLLA
                     GAAVAMAGSEYSDAALTLGSAGAGLILLKFSRNDELEADRLGVEYMTRLGYDPRAALS
                     AHRNLEKVSDQYARSIGQSRQESSFFSELLSSHPRTSIRVEEIQNIINQTPRAAIAGD
                     GTARQRFQGAVANIRRVNAVYVNYYDKAVRSLEKNNTGEALSFVRQAIAQDPGQAPFY
                     ALYGSILVKQNNLPEAERYFSKALSIDGNYQPALRGMGAIRYAGNRYSESVQYLQRSL
                     KLFPQDPASNYFMGMNYYRTRSYRYAIGYLKPFSEAAPRHPRIHAILGQCYEAVGDYQ
                     SAYGQYTMQLKVAPNNDAGGVARSRSRAIEMQFAPKK"
     gene            complement(5486..5800)
                     /locus_tag="PKN85_00620"
     CDS             complement(5486..5800)
                     /locus_tag="PKN85_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNT42935.1"
                     /translation="MSVLFEFAMFPTDKGESVSAFVSRLLRVIDEGTAAYRLTPMGTV
                     VETDTMDQALSIIKKCYEQLEPDCGRVYSTVKFDIRKGASGRLEGKIASVEAKVGKKL
                     KT"
     gene            5908..7065
                     /locus_tag="PKN85_00625"
     CDS             5908..7065
                     /locus_tag="PKN85_00625"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNT42936.1"
                     /translation="MAARRNGFRIFVVSAIVALCLALIAAVFLVKYANMIARAGIEKA
                     MGKDFSIGSIELKWGSVRVHGVAMKDRAGKEVIRVGNLVVKASFMSLLRQKYVISSVL
                     MEKPYIYVEIDRKGDLVSPVFPAGDEIARKAGRKAGDSRRPEGKQRPVILKKIVIVNG
                     SVDYLDRKSPKVPVLTRVRSINAEVRNLTVPFTDDLTHYVLRAAIPEGRSTASVKSEG
                     GIRFQSKDMECTAAVRELDITHFKPYYHGSQSINMTRGLLDLDVRAKIVSRRIHAPGK
                     AVLKGLEFGSGHGLGDRFMGVPVTLVVAFLKKSGDQIPVDFVISGDLDNPKFNIAENF
                     ASRLSFSIAEKLGFSVKDIGESVFGLGTEGTKKIGESIEGIGEGIKKIFKR"
     gene            complement(7216..7446)
                     /locus_tag="PKN85_00630"
     CDS             complement(7216..7446)
                     /locus_tag="PKN85_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42937.1"
                     /translation="MDDILEEERKLRRLRFVVDFALEFIRTQDIPHDHAMHIVEGVRK
                     QALKLFPGKDETFDIIYAPRFKRALNEKYKRD"
     gene            complement(7491..7967)
                     /gene="lspA"
                     /locus_tag="PKN85_00635"
     CDS             complement(7491..7967)
                     /gene="lspA"
                     /locus_tag="PKN85_00635"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNT42938.1"
                     /translation="MRRYPFFALIPVIFALDRLTKALIMRDLPVMSEIKVTSFLSIVH
                     VRNLGGVFGILNESGYAKYIFTVVPVLVAAALVYILIRYTMGKAKTFALCLILAGALG
                     NIYDRIFYGSVVDFLDFHYGKYHWPAFNVADIALSIGIGLLILLELTEAKGRQPSG"
     gene            complement(7940..>9111)
                     /locus_tag="PKN85_00640"
     CDS             complement(7940..>9111)
                     /locus_tag="PKN85_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT42939.1"
                     /translation="DILDVWFDSGVSWAAVCKKRRELEYPADLYLEGSDQHRGWFHSS
                     LLTSVGNEGHAPYKAVLTHGFVVDGSGRKMSKSLGNVIAPNEVIEKFGAEILRLWVTY
                     EDYRDDIKISKDIINRLVETYRRIRNTLRFLHANINEDFEPGRDTVPYEGLSLLDRWL
                     LSRLARLIDRVRESYDNYTLHSIYHAVHNFCTVDLSALYLDIVKDRIYVEHADARKRR
                     ASQTVMYETLTTLVKLIAPVLSSTADEMWSYLKGYVAEESVFLAQFPAADRNLINGVI
                     EEKWERIWRIRELANKKIEEKRTAKEIGHSLDTKVIIDAPDEDHAALMELGDELKDVF
                     IVSQIEVRKAAALDVTVVRAEGDKCERCWQYDTNILRDGKFPNVCKRCADTLSLL"
BASE COUNT         1875 a         2558 c         2713 g         1965 t
ORIGIN      
        1 ccccttcaat gccttcccag gagagggaaa aaggcgtgaa gatctcgtgc ctgagaactt
       61 cgtggagcgg ctggggagcg acgtcgtcgg ccacggggag ggagtggatg tcatccggct
      121 tgtggacggt gtctttcagc tgggcgacaa aggacgcgag ggactgtatg gcatcggtga
      181 aacggccgga gagtctcgcc ccttcctcga cgatgaatgc cgcgtaggag ggggatatct
      241 caaccaggaa agcgggctct ttcgtgccgc tcctgtaggc ggcaacgatc tcgttgagcg
      301 accttctgtc caccggcgaa ctcatgagtt cgcgcaggcc ctcgcggaaa tgcagatcgc
      361 cgttgaggaa gacgaaggta ttcttcctga cctcgtacgc ggccatgacc attcgggagc
      421 cggcatggtc aaagttcgtc aggaagccca tgttggtccg cgactcttcc gccttcctga
      481 ggacgggctc gccttccggg cggagatctt ccacctttac gcccacggac ctcaggcgga
      541 atatggtctt tctgatgagc ttgcggatct ccttatccgt ctcctggaga tagatggcat
      601 taagaagaac gccggtctcc tcgttccttt cctttcccag gccctcgagc accgaaaccc
      661 tttcgtcgcc cgtgagggtg aagtagtact ccactttttt ttcgagatcc tgggcgctgt
      721 cgatcgccct gaccaattca tccatgatcc gccgtgtcct tttctctcga tctcaatgct
      781 tgacggagaa ccccgccttc tcgacggccg cttcgaggtc cttctggctc gccttgccct
      841 cgtcatacct gaccacggcg cttccgatgt ccacatcact cgagtcgacc ccggcgacag
      901 cgccgagggc ctttttgacc gccataacac aatgctggca gctcatgccg tctattttaa
      961 gtttcgcctc agccatcgtg tcctcctttt tgcatccatt ataacacaaa agacggcttg
     1021 aatcgcttga gcggcttgaa tcgcttgagg gctaaaagaa agaaccttac agttttgtcg
     1081 ctctcagcta ttcaagcgat tcgagccgct caaactgtct cttcctcttc caactcgttc
     1141 gtgagctggg cgatcaccag gcagtggttc gatatgtgct ccaggttgac aaggacatcc
     1201 acatatatgg gtccggcctc ggcgagacag agctttttgt agaacctctc caggtgcttg
     1261 cccgtggcgt tcctgatctt tacgtctatc ctgtgttccc gttcgacgat gaggcgtacc
     1321 aacgcgatat ccttcttctc gatgagccgg ccggcgtccg ccaggttgtc gagcaccagg
     1381 gtgcctatgt cccgaagctc ctcgttggcg gccggtgtga aatgggcttt gcgcctgaac
     1441 ttctgttccg ccagttcacc gaggttcatg gagcggtctc ccatgcgttc gatgtcatag
     1501 gcaaatgagg aaaaggcgaa gagcttcttc gtgaggggct gtgaaagggc gccgcaggag
     1561 atgccccaga ggtatgtcgt gatctcggtc tggaggttgt ccatgacgag ttccatgtac
     1621 atgacgttct gtcgcttgtt cctcctgtat gcgacgaaaa gttcgaggga ttccaggagc
     1681 atacgctgtg ccagcgcgat ctgccgtttc agctcgttcc tgacgcagac aagggccatg
     1741 tcggggttgc ccaggtgttt cgtgttcagg tattccggcc acatggcaat gacggcgtgc
     1801 tccccgggga tgatcctgcg gatcgcgttc gaaaaagggg tgaggagaaa catgaagatg
     1861 aagacgatga cgatattgaa aagaaaatgg cagagggcca cctgctgggc aatgctcgac
     1921 gacatcgcct ttaacacagt gacgaagggc gacaggaaaa gcatgcacac cagggcgccg
     1981 gaacacttga agatgaggtg agccagcgct gtccgtctgc cgttgacatc gctcgcgagg
     2041 ctgcctatga gggccgtcac cgtggtgccg acgttcgctc cgatgactat ggggaccgcg
     2101 ttgtctattg acatgagccc ctgcataccg agcatgacga gcatgctgat tgggatggcc
     2161 gacgcctgta cgatccccgt gaagacgaga cccaggagga gagccagcag ggggttctgc
     2221 gcggaaagga aatagcggcc gaagacggga ctgtccttga gcggggccgc tgcgtcccca
     2281 atgaggctca ggccatagaa tatggtgccg aaatagagaa gcgcctcacc gacggtcctc
     2341 acgcggtccc tgccgagaaa gtacagcacg atcccgctga agaggaagag gggggatatg
     2401 tccgtcacct tccacacgac gagctgtacg gtgagcgttg tcccgatgtc ggcgccgagg
     2461 atgatcccca gtgagtgata gaagctgacg agcccggcgc tgacgagccc catggtgagc
     2521 agcgtcgtcg ccgagctgct ctgaaagaag atcgtggtca cgaaccccgt gatcagcccg
     2581 tagaacggct tccggacgga aaaacggata tactcccgta tccgggagct gaatacccgc
     2641 tgcatctgct cgctaagctt gagcatgccc aggaggaaaa gggccagacc tgccagaaaa
     2701 agaaaaacgt aatggatcat cccatatcag gatatcttgc tgccgttagg tatggccttg
     2761 tcgggcgtga ggagcacgac ggaggactta tcttcgctgg aggcggcaag gagcataccg
     2821 tgggacatga tgccccgaag cttgcggggc tccagattgg ccacaacggc gatcttcttc
     2881 cccacgagct cttccttcgt gtagtactgc ttgataccgg cgacgatcag acgttcttcg
     2941 cccatgtcga tggtcagctt gtagagcttg tccgccccct cgatatcttc gcaggcccgt
     3001 atctcggcca ccctcaggtc gatcttgatg aaatcgtcaa acgtgatatt ctccatagct
     3061 gctccttggg ttcaatgacg ccggtctcag tcccggcggt cagggtagtc acggcacggc
     3121 ggccgtgacc gtgttcgtaa gggtccctat gccctcgatc tcgacctcga cggtgtcgcc
     3181 ggggagaaga gagcccaccc cgggaggcgt tccggtaatg atgacgtcgc cgggtaaaag
     3241 ggtcattatc cccgagatga aggacacaag gtcatagacg tcgaagacca tgttggctgt
     3301 tgtcgaccgc tgcttcaccc caccgttgac gcgcgtcacg atctcgaggg aggtcgggtc
     3361 tatgtccctg acgatccgcg gtcccagggg acagaatgtg tcgaaggact tggcccgtgt
     3421 ccattgtccg tccatccgct gcaggtccct tgcggtcacg tcgttggcgc aggtgtaccc
     3481 ggcaatgaaa tcgcgggctt caccgacggg aacatttcgc gcgcgtttgc cgatgacgat
     3541 tccgagttct ccttcgtaat ggagctcctg cgtctgcgcg gggaaaacga tggggtctcc
     3601 gtcaccgacg acggacgtgg acggtttcat gaagatgagc ggatagtcgg gaaggtccat
     3661 cttcagttcc ctggcatggt ccctgtaatt gagccccacg gcgacgacct ttgaagggtt
     3721 gaagcttatg cccatgatgc ctcccggatc gtgtccatgc tgttctattt tacgctttcc
     3781 ccatgttcgg ctgtcaagct atttcctttc ttgacaaagg tccgcgggga acataagaat
     3841 gtagggtaga ctacggagaa aggggcggaa atgtcaatca catcgtttcg tacgttattc
     3901 tatcatgtcc ttctgctggc ggtcgttgcg ctcgccgccg ccggctgtgc catcaatccc
     3961 gtgacgggcg agcaggaatt gatgcttgtc tccgagagac aggagataca gctcggcaac
     4021 gagctctacc ccaacgccct gtggagcgga gaaggcggcg gtggcgaatt cagggacccg
     4081 gcgttgaaat cctacctgtc gagccttgtc gtgaacattc accgcgtgtc gcacaggccc
     4141 aacctccccg tcgattttgc cgttcagaac acctccgtgc ccaatgcctg ggcgataccg
     4201 ggtcatgtcg tgatgacccg gggccttctc gcggcgctcg acaatgaggc ggagtttgtt
     4261 tacgtcatgg gccacgagat ggggcatgtt tcagcgcgcc attcggcgag gcagatgacg
     4321 tacggcatgc tcggccagtt ccttctcgcc ggtgcggcgg ttgccatggc cggctccgag
     4381 tactccgacg cggcgctgac cctggggtcc gcgggtgccg ggctcattct tctcaagttc
     4441 agcaggaacg acgagctcga ggcggacagg cttggcgtag agtacatgac gaggctcggc
     4501 tacgacccgc gggcggcctt gagcgcccac aggaaccttg agaaggtctc cgaccagtac
     4561 gcgcggtcca tcgggcagag caggcaggaa agcagctttt tcagcgaact cctgtcttct
     4621 catcccagga cctccatccg tgtcgaggag atacagaaca tcatcaacca gacgccccgg
     4681 gcggcaatag ccggcgacgg cacggcacgg cagcgcttcc agggggccgt ggccaacatc
     4741 cgtcgcgtta acgcggtcta cgtgaactac tacgacaagg ccgtccgctc ccttgagaag
     4801 aacaataccg gtgaggccct gtccttcgtc aggcaggcca tagcccagga ccccgggcag
     4861 gcgcccttct acgcgctcta tggcagcatc ctggtgaagc agaacaacct tcccgaggcg
     4921 gagcgctact tttcaaaggc gctcagcatc gacggcaact atcaacctgc cctgagggga
     4981 atgggtgcca tcaggtatgc cggtaacagg tattcggaga gcgtgcagta cctccagagg
     5041 agcctgaagc tttttcccca ggacccggca tcgaattatt tcatgggaat gaactactac
     5101 aggacgcgtt cctacaggta cgccataggc tacctgaagc ccttctcgga ggccgctccc
     5161 aggcatccca ggatccacgc catcctggga cagtgctacg aggcagtggg cgactaccag
     5221 tcggcatacg gccagtacac catgcagctc aaggtcgccc cgaacaacga cgcgggcggt
     5281 gtcgcgcgct cacgctcccg ggccattgag atgcagttcg cacccaaaaa ataaagacgg
     5341 cttgaatggc ttgaatcgct tgaatcgctt gagagtcaaa agaagaaagc catagcccgc
     5401 tgttgctccg gcggccggct aaatccgggc attgcttcta taaccctcaa gccactcaag
     5461 cgattcaagc cattcaagcc gtctttcagg ttttcagttt cttccccacc ttggcttcga
     5521 cggaggctat cttgccttcg agcctgcctg aggcgccctt gcggatatcg aacttgacgg
     5581 tggaatagac gcggccgcag tcgggttcca actgctcgta gcactttttt atgatggaca
     5641 gtgcctggtc catggtgtct gtctccacca cggtgcccat tggagtgagg cggtaggcgg
     5701 ccgtaccctc gtcgatgacc ctgaggagcc tcgacacgaa ggcgctgacg ctctcgccct
     5761 tgtctgtggg aaacattgcg aattcaaata gaacggacat gaaggtcctc ctttcccggt
     5821 tacgggaagc cgcggcggtt ctcctgtctt tccttccggg ttcccgttct gtatactagt
     5881 ataaaacatt tcgaaaagga tgggaaaatg gctgccaggc ggaatgggtt caggatattc
     5941 gttgtctctg ccatcgtggc tctctgcctt gccctgatcg cggccgtctt tctcgtgaag
     6001 tacgccaaca tgatcgccag ggccggtatc gagaaggcca tggggaagga cttctctata
     6061 gggagcatcg aactgaaatg gggaagcgtc cgtgtccatg gtgttgcgat gaaagacagg
     6121 gccggcaaag aagtgatacg ggtcgggaat cttgtggtca aggccagctt catgagcctt
     6181 ctcaggcaga agtacgttat ttccagcgtc ctcatggaaa aaccttacat atacgtcgag
     6241 atcgacagaa aaggcgatct cgtaagtcct gtcttcccgg ccggtgacga aatagcaagg
     6301 aaagccggga gaaaggccgg ggatagccgc cggcccgaag ggaaacagcg tcctgtgata
     6361 ctcaagaaga tcgtcatagt aaacgggtcc gtggactatc tcgaccgcaa gtctccgaag
     6421 gttcccgtct tgacaagggt gcgaagcata aacgcggagg tgcgaaacct gacggtgcct
     6481 ttcacggacg atctcactca ttacgtcctc agggccgcca tccctgaggg caggagcacg
     6541 gcgagcgtga agagcgaagg cgggatcagg ttccagagca aggacatgga gtgcacggcg
     6601 gcggtaaggg agcttgacat cacgcatttc aagccctact accatggaag tcagagcata
     6661 aacatgacca ggggtcttct tgatctggac gtgcgggcga agatcgtttc ccgcaggatc
     6721 cacgcgccgg gaaaggcggt gctcaagggt ctggaattcg gaagcggcca cggtctgggt
     6781 gacaggttca tgggtgtgcc cgtcacgctt gtcgtggcct tcctgaagaa gagcggggat
     6841 cagatccccg ttgatttcgt catttcaggc gatctcgaca atccgaagtt caacatcgcg
     6901 gagaacttcg ctagcagact ctccttcagc atcgccgaaa aactgggttt ttccgtaaag
     6961 gacatcgggg agtcggtctt cggcctcggc accgaaggca cgaaaaagat aggtgaaagt
     7021 attgagggta tcggcgaggg gataaagaag atattcaaac gataaagacg gcttgaatgg
     7081 cttgagtcgc ttgaacagct tgaaagtcac aggaacaacc ttttctggcc atcattcccg
     7141 cgcaccgggg aatccagagg ttgtttcctc aaccttcgaa cgattcaagc gactcaagcc
     7201 attcaagccg tcttttcagt ccctcttgta cttctcgttc agcgcccttt tgaagcgggg
     7261 ggcgtagatg atgtcgaagg tttcgtcttt tccggggaag agcttgagcg cctgcttgcg
     7321 caccccctcg acgatgtgca tggcgtggtc gtgggggatg tcctgggtgc ggatgaactc
     7381 gagcgcgaag tcgaccacga aacgaagccg cctgagcttt ctctcttctt ccaggatatc
     7441 gtccatgacg ttccctcttt atggcttccg gcaccgcctg cgatgtgcct tcagccggag
     7501 ggttgcctgc cctttgcctc ggtcagttcg aggaggatga ggagcccgat gccgatggaa
     7561 agggctatat cagccacgtt gaaggcgggc cagtggtatt tcccgtagtg gaagtcgaga
     7621 aagtcgacga cgcttccata gaatatgcgg tcgtatatgt tgcccagggc gccggcaaga
     7681 atgaggcaca gcgcgaaggt cttcgccttt cccatcgtgt accggataag gatgtatacc
     7741 aaggcggcgg cgacgaggac gggtaccacg gtgaagatgt atttggcgta cccgctctcg
     7801 ttgaggatac cgaagacacc tcccaggttc ctcacatgga cgatggagag gaaggacgtc
     7861 acctttatct cgctcatcac ggggagatcc ctcatgatga gcgccttcgt gagtctgtcc
     7921 agggcgaaga tgaccggtat cagagcaaag aaagggtatc ggcgcatcgc ttgcacacgt
     7981 tcgggaattt cccgtccctg agaatgttcg tatcatactg ccagcaccgc tcgcacttgt
     8041 ctccttcggc cctgacgacg gtcacatcga gcgcggcggc cttgcgcacc tcgatctggg
     8101 agacgatgaa gacatccttg agttcgtcgc ccagttccat gagggccgcg tggtcctcat
     8161 cgggggcgtc tatgatgacc tttgtgtcga gagagtggcc gatctccttg gcggtgcgct
     8221 tctcctcgat cttcttgttg gcgagttccc tgatcctcca tatccgttcc cacttctcct
     8281 cgatgacccc gttgatgagg ttcctgtccg cggcggggaa ctgggccagg aaaacgctct
     8341 cctccgcgac atagcccttg agatatgacc acatctcgtc agccgttgat gaaaggacgg
     8401 gcgctatgag cttgacgaga gtggtgagcg tctcatacat gacggtctgg gatgcccttc
     8461 gtttcctggc gtccgcgtgc tccacgtaga tcctgtcctt gacgatgtcc agatagaggg
     8521 cgctgaggtc gacggtgcag aaattgtgga ccgcgtggta gatggaatgc agggtatagt
     8581 tgtcgtagga ttccctgaca cggtcgatga gcctcgcaag ccgcgacagg agccacctgt
     8641 cgaggaggga gagcccttcg tagggaacgg tgtcgcggcc aggctcgaag tcctcgttga
     8701 tgttcgcgtg gaggaaacgc agggtgttgc ggatccggcg gtatgtctcc acgaggcggt
     8761 tgatgatgtc cttcgatatc ttgatgtcgt cgcggtagtc ctcgtaggtc acccacaggc
     8821 gcaatatctc ggcgccgaac ttctcgatga cctcattggg ggcgatgacg ttccccaggg
     8881 acttggacat cttcctgccc gagccgtcaa cgacgaaacc gtgggtcaac accgccttgt
     8941 agggggcgtg gccttcattg cccacggagg tgagcaaaga gctgtggaac catccccggt
     9001 gctggtcgct cccctcgaga tagaggtcgg cagggtattc caactcccgc ctcttcttgc
     9061 agacggcggc ccaactcaca cccgaatcga accatacgtc gaggatgtcc t
//
LOCUS       DAOQTR010000012         8883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_8742_length_8883_cov_3.696420, whole genome shotgun sequence.
ACCESSION   DAOQTR010000012 DAOQTR010000000
VERSION     DAOQTR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_8883_cov_3.696420"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PKN85_00645"
     CDS             complement(<1..1009)
                     /locus_tag="PKN85_00645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT42940.1"
                     /translation="MTHHSSKTNILFILQTFRTGGSERVVVDLCRRLDRGRFNCFAAA
                     LIDGDLRETLEGMGVPTVLPRIRSARQDALNVMREVSDFIGANAIRVVNAHHFTPFLY
                     GSYGARRHGCRTFYTVHSRREVEVMDRSWRVLGGVLVRLADGVIGISPDVSEAVKRQF
                     RLPDAKVFTLTNAVDHTRFAVDVDTRAKRRELGIGENDRVIGCVGNLRWDKNYPNLIR
                     AFGLLAERARDARLVIAGEGKRKDDLERLIAGTGLKGKVLLLGARDDVPEIMKTMDVY
                     CLSSFREGLPLSLLEAMSAGLPVVGTDVPGIRDVIVDGDTGLLVPSDDPARLSEALFK
                     VL"
     gene            complement(1016..2182)
                     /locus_tag="PKN85_00650"
     CDS             complement(1016..2182)
                     /locus_tag="PKN85_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT42941.1"
                     /translation="MDVPAYVTTVFWAAVLIVLYAYAGYPVVLAVLGAFRASRPVSSG
                     TDGLPKVSLLISAYNEAAVIEDKLVNSLDLDYPEELLEIVVISDASCDGTDDIVRKYR
                     DRGIVLVRCEGHIGKTACLNRAVPQAAGDIIVFSDANSRYDRRAVRCLVERFTDRKIG
                     FVTGRTEYLPPAGDGGPSPAGLYTRVEGLTKALEGRIGSCVGADGAIFAIRKSLYRPL
                     DASDINDLVIPARIVQQGYRGVLAPDAFCLEPAAPDVSGEFARHVRITTRSLRAIFRH
                     SALLNPFRYPLFSFELASHKLIRFLVPFALFTAFVLNVLIVAAGGGRVYGATLLLQAC
                     LYVLWFAGSLNIPLSFLTRPSRAAHTFCAVNLAILIGWIKFLKGEKFTTWATQR"
     gene            complement(2185..3444)
                     /locus_tag="PKN85_00655"
     CDS             complement(2185..3444)
                     /locus_tag="PKN85_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT42942.1"
                     /translation="MRIRRTLSPTAAPLRPADLWRGLKGFFAGEKYLRRLKEEITGYF
                     GVRHAWFTSSGKAALYVILRALRSLEPDRTEVLIPAYTCFSVPSAIVRAGLEVALCDI
                     DASSLDLNGTLLGKSVNEKTLCVVPDHLFGIPSAMGSIVDLCRDRGVFVLEDAAQAMG
                     GRCDGRFLGTIGDVGLFSLGRGKPVACGSGGIIITASDVIAGAIEREFSSLPFPGRAT
                     QAGEYLKAAAMSLFIHPLLYSLPAALPFLHLGQTVFDSRFPVMRFSSMQAGLCMTWRR
                     RLEAAMSVRKRQAAMLCRMLDAARIAGDVSSAGYLRLPFMTESRERRQRVCRTAKKLG
                     LGITTMYPSPVNEIPQIAHRFAGSTFPAARLVADRLITLPLHRYVRDGDREEMARLLK
                     PVRSIGVRSGVLSVDMTVPGMTGKERV"
     gene            complement(3486..4520)
                     /locus_tag="PKN85_00660"
     CDS             complement(3486..4520)
                     /locus_tag="PKN85_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNT42943.1"
                     /translation="MTVHFYNESDRDDWDRYVVDHNSASCYHLTGWKDVIEESFGHRT
                     FYLLSRDEAGAIDGILPLVHLNSLLFGNFAVSLPFFNYGGICADSDGIRDGLFEEAAR
                     FARTRGMAHLELRHSEDLLPGVPVKTSKVSMRLALPGTKEELNASFPSKLRSQVRRAV
                     KEGMVARMGGQEELGSFHSVFSRNMRDLGTPVYSRRFFRAILERFPDAARICTVYTRE
                     GMPVASGMIVGFRDTLEIPWASSLKDFNRYGPNMLLYATVLGFACDAGYRVFDFGRST
                     PDEGTYRFKEQWGAKPAQLYWHYWLRNGRPMPEINPRNPKYGLAIRIWQHLPLGLTRL
                     IGPPLVKNLP"
     gene            complement(4560..5600)
                     /locus_tag="PKN85_00665"
     CDS             complement(4560..5600)
                     /locus_tag="PKN85_00665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT42944.1"
                     /translation="MQQDLSIRRYAVGDEPGILKLFAEVFGRNRTAGEWYWQYNDNPW
                     GGGWPVVGVTGNEIVAHYGVARGDLCLRGRRIEAGQSGDAMVRSGMRRNGYCARLMDY
                     CYQLTAADGLKATYGVPNRLSYPGLVRYSGWCKIVNLSYFFFRIGFGKLWGSGPDRVF
                     KCLGGVSSALRMRARRLRHRGDFEISVSSSLPDGLEDLLRNKRTYEVLALWKDPQYLR
                     WRYESHPDVTYLFHTVRTGGIPRALLITRDCGNAIAICDLIHRDNDEVPSLLLLDHIV
                     NYYARSSAQKIEFYGHDAGFFRFVFTSCGFRSIPFSPLVFGGRVFSDPDLDKVFLLPD
                     SWNIAYGDTDVI"
     gene            complement(5603..6565)
                     /locus_tag="PKN85_00670"
     CDS             complement(5603..6565)
                     /locus_tag="PKN85_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42945.1"
                     /translation="MVKVAITIDTEEDLWGEYTPAPCPVDNIDSIPLVQDIFDRYGAI
                     PTYLVDYPVVIDDRASRLLRDIREKGGCELGTHCHPWNTPPYGDNASVKATMLCNLEE
                     DAVRRKMENLHETFVARFGMAPVSFRAGRWGFGPAVARSIQDLGYRVDSSVTPLVDWS
                     DYGGPDFFGAPRHPYRLNPASILEPAEDGSLLEAPPTTGFFQKNAELCGKVFRYVMKG
                     NLTRRLKGIGLLEHSRLINLRWLSPELSSGEDMLLLAERLVRHGTSFLNMCFHSTSLL
                     PGKSPFVRDGKDLRRFLLNIGMFLQYARDAGWNFIALKDIREDH"
     gene            complement(6613..7539)
                     /locus_tag="PKN85_00675"
     CDS             complement(6613..7539)
                     /locus_tag="PKN85_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNT42946.1"
                     /translation="MDSRQKEAKPVMVTVDLEDWFQVENLRPLFPPETWDSCELRVER
                     NVHGLLELFDACRVEATFFVLGWVAERCRGLVREVARAGHEIASHGYSHRLCRELTGA
                     SLRRDIEMSRSLLGDITGEAPAGYRAPNFSITRELTDILAELGFAYDSSYNSFGLNGR
                     YGRAEGLRWGTAGHLVADSGIVELPVSNLTVMGRTVPWAGGGYFRLWPTALFEHGVAR
                     ILQKEGQYVFYCHPWEVDPGQPRQTSGIGWVSAFRHYLNLDRTLHRLEHFLVAFRENR
                     FISCSRYLGVSEYEGAREEHGPGRLREAVPAL"
     gene            complement(7545..>8883)
                     /locus_tag="PKN85_00680"
     CDS             complement(7545..>8883)
                     /locus_tag="PKN85_00680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNT42947.1"
                     /translation="AAASEGTLGLMAIIAAIGLRVGFGWKALLHYSIGPVSTLMLYPL
                     ALYIFDLYNVERVFRSWETTFRSAIAVALGTFLAICVFYVVPLGPYGRGVMIIEASLA
                     WYFLNLWRWSYGIVFQKTGPRTPTLILGAGFCGRTICRLLRSPLSPYEVKGFLDDNLV
                     EIDAEKYPAIMGTSDQLLEAAAGAGAHTVILAIPKNRSVRLIRTILDARLKGIDVRDM
                     ADVYEALTGRIPVRGIADQWLLFAQGFYLLRADYAQKLKRLLDFFASGLILLLMSPVI
                     ALAVIAIKLDSPGPVFYTQQRVGKDRQVFTIYKFRSMEYGCEKAGARWAAVKDPRVTR
                     VGKVLRLTHIDEIPQIWNIFRGDMSLVGPRPERPEFVDLLEDELPYYYVRHTVKPGLT
                     GWAQINYRYGASVEDANIKLEYDLYYVKNMSIFLDLKILLRTIGVVLLKDGSR"
BASE COUNT         1776 a         2765 c         2705 g         1637 t
ORIGIN      
        1 cgagcacctt gaagagggcc tccgagagcc ttgcggggtc atcggacggc acgagaagac
       61 ccgtatcgcc gtcgacgatg acgtcccgta tacccgggac gtccgttccc accacgggaa
      121 gaccggcgga catcgcttcg aggagggaaa gaggcagtcc ttccctgaaa gagctcaggc
      181 aatagacatc catggtcttc atgatctccg ggacgtcatc cctggcaccc aggagaagca
      241 cctttccctt gaggcccgtc ccggcgatga gtctttccag gtcatccttc ctcttccctt
      301 cgccggcaat gaccagtctc gcgtcgcgcg cgcgttccgc gagcagaccg aacgccctta
      361 tgaggttggg atagttcttg tcccacctga ggttgccgac gcacccgata acgcggtcgt
      421 tctcccctat tccaagttcc ctccgcttcg ccctcgtgtc gacatccacc gcgaaacggg
      481 tgtggtcgac ggcattggta agggtgaaca ccttcgcgtc gggcaggcgg aattgccgtt
      541 tgacggcttc gctgacatcg ggggatatcc ctatgacccc atcggccaga cgcacgagaa
      601 cgcccccgag aacacgccag gacctgtcca tcacttcgac ctccctgcgg ctgtggaccg
      661 tgtagaaggt cctgcagcca tgacgccttg ccccgtaaga accgtagagg aagggtgtga
      721 aatggtgggc gttcaccacg cggatcgcgt tggccccgat gaagtccgaa acctctcgca
      781 tgacgttcag cgcgtcctgc cgtgccgatc gtatccgcgg caggaccgtg ggaacaccca
      841 tcccctcgag tgtttcgcgc aggtccccgt caatgagagc tgccgcgaag caattgaacc
      901 tcccccggtc gagacgacgg cacaggtcca ccacgacccg ttccgacccc ccggtgcgga
      961 aggtctggag gatgaagagg atgttggtct tagaggaatg gtgggtcatc ggtctttacc
     1021 gttgcgttgc ccaggtggtg aatttttctc ccttgaggaa tttgatccag ccgatgagaa
     1081 tggcgaggtt cacggcgcag aaggtatgtg ccgcgcggga cggacgcgtg aggaaggaga
     1141 gggggatgtt gagggacccc gcgaaccaca ggacatagag gcaggcctga aggaggaggg
     1201 tcgctccgta tacgcgccct ccgcccgcag ccacgattag gacattgagg acaaaggccg
     1261 tgaagagcgc gaaggggacg aggaaccgta tcagcttgtg cgaggcgagc tcgaaggaga
     1321 aaagagggta tctgaagggg ttcaaaaggg ccgaatgcct gaagatggcg cgcagcgacc
     1381 ttgtcgttat cctcacgtgc ctcgcgaact caccggacac gtccggggcg gcgggctcga
     1441 gacagaacgc gtcgggggcg aggactcccc ggtaaccctg ctgcactatc ctcgcgggta
     1501 tgacgagatc gtttatgtcg ctggcatcga gaggccggta gagagacttg cggatggcga
     1561 atatcgcgcc gtccgccccg acgcaggagc ctatcctgcc ctcgagggcc tttgtgagtc
     1621 cctcgacgcg ggtgtacaat ccggcgggag acggtccgcc gtcacctgcc ggtgggaggt
     1681 attccgtcct gcccgtcacg aacccgatct tcctgtcggt gaacctctcc accaggcatc
     1741 taaccgcccg gcggtcgtag cgcgaattcg catcggagaa aacgatgatg tcacctgccg
     1801 cctgaggcac ggccctgttc agacaggccg tcttgccgat gtgcccctca cagcgtacga
     1861 ggacgatccc ccggtccctg tacttccgca cgatatcgtc cgtcccatcg caggacgcgt
     1921 cggagatgac cacgatctcg aggagttcct ccggatagtc gagatcgaga gaattcacca
     1981 gtttgtcttc gatcacggcc gcctcgttgt aggcggaaat gaggaggctc accttcggaa
     2041 gaccatccgt gcccgaagag acgggccggc tcgccctgaa agcaccgagg accgcgagga
     2101 caacgggata gcccgcgtag gcgtaaagaa cgatgagaac ggccgcccag aaaaccgtgg
     2161 ttacgtatgc cggaacatcc atggtcacac cctttccttc cccgtcatgc cgggcacggt
     2221 catatcgaca ctgaggacgc cgcttcggac ccctatagac cgcaccggct tgagaaggcg
     2281 ggccatctcc tcccggtccc catccctgac ataacggtgc aggggcagcg ttatgaggcg
     2341 gtccgcgaca aggcgggccg cgggaaaggt cgatccggcg aaacggtgcg ctatctgggg
     2401 gatctcgttt accggcgagg ggtacatcgt ggttatcccc aggcccaatt tcttcgcggt
     2461 cctgcatacc ctttgccggc gctcccggct ttccgtcatg aagggaagcc tcaggtagcc
     2521 ggcggacgac acgtcgcccg cgatccgggc ggcatcgagc atccggcaga gcatcgccgc
     2581 ctgccttttc cggacggaca tggcggcctc cagacggcgg cgccaggtca tgcacaaccc
     2641 ggcctgcatg gaagagaacc tcatgaccgg gaaccggctg tcgaagacgg tctggccgag
     2701 gtgcaggaaa ggaagggccg cgggcaggga gtacaggaga ggatggataa agaggctcat
     2761 cgccgccgct ttcaggtatt ctccagcctg cgtcgccctg ccggggaatg gcagcgacga
     2821 gaattccctc tctatcgctc cggctatcac gtcggaggcg gtgatgatga tcccccccga
     2881 tccgcaggcg acaggctttc cgcgtcccag gctgaaaaga ccgacgtcgc cgatggttcc
     2941 caggaacctc ccgtcacacc tgccgcccat tgcctgggcc gcgtcctcca gaacgaagac
     3001 acccctgtcc ctgcaaagat ccacgatact ccccatggct gaagggatac cgaagagatg
     3061 atcggggacg acacagagcg tcttctcgtt gacgcttttc ccgagaagtg tgccgttgag
     3121 atcgagcgac gaggcgtcga tgtcgcacag ggcgacctcg agccccgcgc ggacgatggc
     3181 ggatggaacg gaaaagcacg tgtacgcggg tatgaggacc tccgtcctgt cgggctcaag
     3241 cgatcgcaac gcgcggagga tgacgtacag ggcggccttg ccggacgagg tgaaccaggc
     3301 gtgtctcaca ccgaagtatc ccgtgatctc ctccttgagc cttctgaggt atttctcccc
     3361 tgcgaagaaa cctttgagcc ccctccagag gtccgcgggg cggaggggag ctgcggtcgg
     3421 ggatagggtt cgccggatcc tcatcagctg tgtcttccgt ggcagggata gtccgtcatg
     3481 gtacgctagg gaaggttctt cacgaggggc gggcctatga gccgcgtgag ccccagggga
     3541 agatgctgcc atatcctgat cgccaatccg tacttcggat tgcgcgggtt gatctccggc
     3601 atcggccggc cgtttctcaa ccagtaatgc cagtacagct gcgctggttt tgcgccccac
     3661 tgctctttga acctgtaggt gccttcatcg ggagtggaac ggccgaagtc aaagaccctg
     3721 tagcccgcgt cgcaggcaaa ccccagcacg gtggcataca ggagcatgtt gggaccgtaa
     3781 cggttgaagt ccttaagcga cgacgcccag gggatctcca gcgtatccct gaacccgacg
     3841 atcatccccg aggccaccgg cattccctcc ctcgtgtaca ccgtgcatat ccgggccgcg
     3901 tcagggaacc tttcgagtat ggcgcggaag aacctcctgg aatagaccgg cgtccccagg
     3961 tccctcatgt tccgcgagaa aacgctgtgg aaactcccga gttcttcctg cccgcccatg
     4021 cgggcaacca tcccctcctt taccgctctt cgcacctggc tcctcagttt tgaagggaaa
     4081 gaggcattga gctcctcctt cgtcccggga agcgcgagcc tcatggacac cttcgatgtc
     4141 ttgacgggaa ccccggggag gaggtcctcg gaatgcctca gctccaggtg ggccattccc
     4201 cgcgtccggg cgaagcgcgc ggcctcttcg aaaagaccgt cacggatgcc gtcgctgtcg
     4261 gcacatatgc cgccgtagtt gaagaagggc agggagaccg cgaagttgcc gaaaaggagg
     4321 ctgttcaggt ggaccagggg cagtattcca tcgatcgcgc ccgcctcatc cctcgacagg
     4381 agatagaagg tcctgtgccc gaagctttct tcgatgacgt ccttccaacc ggtcaggtgg
     4441 tagcagctcg cgctgttgtg gtccacgacg taacggtccc agtcgtcacg gtcggattcg
     4501 ttataaaagt gaactgtcat gtctctcctc gatgcgtctc ggaaggaagc ggcgggcgct
     4561 cagatcacgt ccgtgtctcc ataggcaatg ttccagctgt cgggcagaag aaagaccttg
     4621 tcaaggtccg ggtccgagaa cacccgccct ccgaacacga ggggcgagaa gggtatgctc
     4681 ctgaacccgc aggacgtgaa gacgaagcga aagaaaccgg cgtcgtgccc gtagaactcg
     4741 atcttctgcg ccgatgagcg cgcgtaataa ttgacgatat gatccagcag caagagggac
     4801 ggcacttcat cgttgtccct gtggataagg tcacagatgg cgatggcgtt gccgcaatcc
     4861 cgcgttatca gaagggcccg gggtatcccg cctgttctca ccgtgtggaa gagatacgtg
     4921 acatccggat ggctctcgta ccgccatctg aggtattggg ggtccttcca cagggccagg
     4981 acctcgtatg tcctcttgtt gcgcaaaaga tcttccaggc catcgggcaa cgaagacgac
     5041 acggatattt caaagtcccc gcggtggcgc aaccttcgcg cgcgcatccg caacgccgac
     5101 gagacgccgc ccagacattt gaacacccgg tccggaccgg aaccccagag cttcccgaac
     5161 ccgatccgga agaagaaata actcaggttc acgatcttgc accatcccga gtacctgacg
     5221 agcccgggat atgacagcct gttgggaaca ccgtacgtcg ccttgaggcc atcggcagcg
     5281 gtgagctgat agcagtagtc catcaggcgg gcacaatacc cgttccgtcg catgccggag
     5341 cgcaccatcg cgtcccccga ctggccggcc tcgatccgcc gccccctcag gcacaggtcc
     5401 ccccgcgcaa caccgtaatg cgcgacgatc tcgtttcccg tcacacccac gacgggccat
     5461 ccgccgcccc agggattgtc attgtattgc cagtaccact caccagccgt cctgttcctt
     5521 ccgaagacct cggcgaagag cttcaggatg cccggctcgt caccaacggc ataccgtctt
     5581 atggacaggt cctgttgcat cgtcagtgat cctcccttat gtctttcagc gcaatgaaat
     5641 tccagccggc gtcccgcgcg tactgaagga acatcccgat gttgagcagg aacctccgca
     5701 ggtctttccc gtcccgcacg aaggggctct ttccgggaag gagcgatgtc gaatgaaaac
     5761 acatgttgag gaacgacgtt ccgtggcgca ccaatcgctc cgcaagcagg agcatgtcct
     5821 ctcccgagct cagttcggga gagagccacc gaaggttgat gaggcggctg tgctccagca
     5881 ggcctatgcc tttaaggcgc ctggtgaggt tccctttcat gacgtacctg aagacctttc
     5941 cgcaaagctc ggcgttcttc tgaaaaaagc ccgttgtcgg cggcgcctcc agtagactgc
     6001 catcctccgc aggttcaagg atgctcgcgg ggttcaggcg gtacgggtgc cgcggagcgc
     6061 cgaagaaatc cggccctcca tagtcggacc aatcgacaag gggcgtgacg gagctgtcca
     6121 ccctgtaacc caggtcctga atgcttcgcg cgacggcggg accgaagccc cacctgccgg
     6181 cgcggaaact caccggcgcc atcccgaatc gggccacaaa agtttcgtga aggttctcca
     6241 tcttcctcct gacggcgtct tcctcgagat tacacagcat ggtcgccttc acgctcgcat
     6301 tgtccccgta gggcggcgtg ttccagggat ggcagtgggt tcccagttca cacccgcctt
     6361 tctcccgaat gtctcgaagc agacggctcg cacggtcgtc gatcacgaca gggtaatcca
     6421 cgaggtatgt cggaatggca ccgtaccggt cgaagatgtc ctgcaccagc ggaatggaat
     6481 ctatgttgtc cacgggacag ggggccggcg tgtattcgcc ccagaggtct tcctcggtat
     6541 ctatggtaat ggcaacctta accatgtcgc ttcaaaggat atccaccgcg gtacccttca
     6601 ttccgcgcgg tgtcaaaggg ccggaactgc ttcccggagg cgccccggtc catgttcttc
     6661 ccgggcccct tcgtattcac tgacccccag atagcgagag catgaaatga agcggttctc
     6721 ccggaaggca acgagaaagt gttccagtct gtggagggtc ctgtcgagat tgaggtagtg
     6781 cctgaaggcg ctgacccacc ctatgccgga ggtttgacgg ggctgtcccg gatccacctc
     6841 ccaggggtga cagtagaaga cgtattgtcc ttccttttgg aggatgcggg cgacgccatg
     6901 ctcaaagagg gccgtcggcc agagtctgaa atagccgccg cctgcccacg ggacggtccg
     6961 ccccatgacc gtgaggttgc tcaccggcag ttccacgatg ccgctgtccg ccacgagatg
     7021 ccccgcggtg ccccaccgga ggccttcggc ccgtccgtac cggccgttga gaccgaaact
     7081 gttgtaactt gaatcgtagg caaaaccaag ctccgccagg atgtccgtaa gttcccgcgt
     7141 gatggagaaa ttcggcgccc ggtaacccgc cggggcctct ccggtgatgt ccccaagcag
     7201 cgacctgctc atctcgatgt ccctgcgaag ggaagcgccc gtgagctcgc ggcacagccg
     7261 gtgggagtag ccgtgtgaag ctatctcgtg gccggcacgc gccacctccc ggacaagccc
     7321 ccggcagcgc tcggccaccc agccgaggac aaagaacgtc gcctccacac ggcacgcgtc
     7381 gaagagctcg agaagcccgt gcacgttcct ttcgacgcgc agttcgcagg aatcccacgt
     7441 ttccgggggg aacaggggcc gaaggttctc gacctggaac cagtcctcga gatcgacggt
     7501 gaccattacg ggctttgcct ctttctgtcg tgagtccata atacctatcg cgacccgtcc
     7561 ttcaggagga ccacgccgat ggtgcgcagg aggatcttga ggtcgagaaa gatggacatg
     7621 ttcttgacat agtagaggtc gtactcgagc ttgatgttcg cgtcctccac cgacgccccg
     7681 taccggtagt ttatctgcgc ccaccccgtc aggcccggct tcacggtgtg acgcacatag
     7741 tagtagggca gttcgtcttc caggaggtcg acgaactccg gcctctcggg gcgcggcccc
     7801 acgaggctca tgtcccccct gaagatattc cagatctggg ggatctcatc gatgtgggta
     7861 aggcgcagga ccttgcccac ccttgtgacc cgggggtcct tcaccgcggc ccaccgcgcg
     7921 ccggccttct cgcatccgta ttccatggac ctgaacttgt agatcgtgaa aacctgccgg
     7981 tcctttccca ccctctgctg ggtgtagaag accgggccgg gggaatcgag cttgatggcg
     8041 atgacggcga gagctatcac cgggctcatg agcagcagga tcagcccgga ggcaaaaaaa
     8101 tcgaggaggc gcttgagctt ctgcgcgtag tcggcgcgaa gaaggtaaaa accttgcgcg
     8161 aagaggagcc attggtcggc gatgccgcgc accgggatcc gacccgtcag cgcctcgtag
     8221 acgtcggcca tgtcgcggac gtcgatcccc ttgaggcggg cgtcgagtat ggtgcgtatc
     8281 aatcgcacgg accggttctt cggtatcgcc agaatgaccg tgtgcgcccc cgcacctgcc
     8341 gcggcctcga gaagctggtc gctggtgccc atgatggcag ggtatttttc cgcgtcgatc
     8401 tccacaagat tgtcgtcgag gaaccctttc acttcgtacg gcgacagggg agatctgagc
     8461 agcctgcaga tcgtcctgcc gcagaacccg gccccgagga tcagcgtcgg agtcctcgga
     8521 ccggtcttct ggaaaacgat cccgtagctc catcgccaga gattgaggaa gtaccaggcg
     8581 agagaggcct cgatgatcat gacgcccctg ccatatggac ccagcggaac gacgtagaag
     8641 acgcagatgg caagaaaggt cccgagggcc accgctatgg cgctgcgaaa ggtagtctcc
     8701 caggaacgga atacacgctc cacattgtag aggtcgaaga tgtagagggc aagcgggtac
     8761 agcataaggg tggaaacggg tccgatggaa tagtggagaa gggccttcca cccgaaaccc
     8821 accctgagtc cgatggccgc gatgatggcc atgagcccga gcgtgccctc ggacgccgcg
     8881 gcc
//
LOCUS       DAOQTR010000013         8874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_8752_length_8874_cov_3.988888, whole genome shotgun sequence.
ACCESSION   DAOQTR010000013 DAOQTR010000000
VERSION     DAOQTR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8752_length_8874_cov_3.988888"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..917
                     /gene="hisF"
                     /locus_tag="PKN85_00685"
     CDS             159..917
                     /gene="hisF"
                     /locus_tag="PKN85_00685"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNT42948.1"
                     /translation="MLTKRIIPCLDVMEGRVVKGTNFLELKDAGDPVENAKAYEEQLA
                     DELCFLDITASHEKRRTIIDVVERVSHEVFMPLTVGGGIRSIDDIRDILRAGADKVTV
                     NTTAVENPEFVRESSGIFGSQCICVAIDAKRREGGGFEVYTYGGRRPTGVDAIGWAKR
                     VEELGAGEILLTSMDRDGTRIGFDIELTRTIAESVNIPVIASGGVGTLEHLYEGLATA
                     KADAVLAASIFHYREHTVVDAKRYLRDRGVNVRL"
     gene            987..1367
                     /gene="hisI"
                     /locus_tag="PKN85_00690"
     CDS             987..1367
                     /gene="hisI"
                     /locus_tag="PKN85_00690"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNT42949.1"
                     /translation="MDDLKWDEKGLMPAVVQDASSKDVLMVAYMNREALKLTLKTKTA
                     HYYSRSRQKLWLKGETSGHTQAVKEVRIDCDNDTILLLVDQKGAACHTGYWSCFYRAW
                     SDGWKIVGKKVFDEKEVYGEKKTH"
     gene            1345..1797
                     /locus_tag="PKN85_00695"
     CDS             1345..1797
                     /locus_tag="PKN85_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNT42950.1"
                     /translation="MVKRRPIDAALFFFNTCCFVGCIPGAPGTYASLLAAVLIWLFPA
                     IFGDAVFAVILIVFAVATVTMERFEGEDPGRIVIDEFAGMCVAMAGHRATLLTVAIGF
                     VLFRIFDILKPFPVGRMERLKGGWGVVGDDVVAGIFANILLLLWTRFI"
     gene            1794..2276
                     /locus_tag="PKN85_00700"
     CDS             1794..2276
                     /locus_tag="PKN85_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNT42951.1"
                     /translation="MKIEERLVRTLTARSLSLCTAESCTGGLIAGRITAVPGASACFE
                     GGVVTYSNRAKAILLGVPVEVIEDHGAVSEEVARAMAEGARERLGTDIAVAVTGIAGP
                     AGGSPEKPVGTVFISLAVAGMTSVRGFRFEGTRREIRRRSGDEVLHFVLDHLEGKVNG
                     "
     gene            2273..2821
                     /gene="thpR"
                     /locus_tag="PKN85_00705"
     CDS             2273..2821
                     /gene="thpR"
                     /locus_tag="PKN85_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNT42952.1"
                     /translation="MRSFLAFEIPPEVKAYLSQVSRAMAKTLSGVRWVRPEGQHITIR
                     FFGEISEARAREIEDIVSAAGPYPGVGATLKRIDAFPDKRRPRVIVATIDEGVDIIRS
                     IYHDIENRLTALGYEREKRGFTPHITFGRVKVPAPLLERDTPPLECPRFAIERIILYK
                     SILGREGATYEPLFEKKLGGAA"
     gene            2881..3945
                     /gene="recA"
                     /locus_tag="PKN85_00710"
     CDS             2881..3945
                     /gene="recA"
                     /locus_tag="PKN85_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNT42953.1"
                     /translation="MNPKTLKGGIDVKDDDNRGKAIDMAVSQIEKLFGKGAIMKLGAK
                     ALEAVPVISTGSIALDLALGIGGIPRGRVVEIFGPEASGKTTLALQVVAEIQKTGGSA
                     AFIDAEHALDVSYAKRIGVNTDDLLISQPDTGEQALEISEILVRSGGVDVVVVDSVAA
                     LVPRAEIEGEMGDSHMGLQARLMSQALRKLTAITSKSMTTVIFINQIRHKIGIMFGKP
                     ETTTGGNALKFYASLRLDIRRIAAIKDGQEVIGSRTRVKIVKNKLAPPFREVEFDIIF
                     GEGISREGDVVDLAVEMGIVEKAGTWYSYGDSRIGQGRENAKEYLKTHPETMKEVEGK
                     IMIQAGLKKGETGDIATDQK"
     gene            3920..6553
                     /gene="alaS"
                     /locus_tag="PKN85_00715"
     CDS             3920..6553
                     /gene="alaS"
                     /locus_tag="PKN85_00715"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNT42954.1"
                     /translation="MTSQQIRNKFLEYFARNGHTIVPSSGLLPEKDPTLLFVNAGMVQ
                     FKNLFLGLEKRPYVRGTSCQKCVRAGGKHNDLDNIGKTLRHHTFFEMLGNFSFGDYFK
                     KEAIAFAWEFLTKELGLDASKMWVTVFREDDEADELWRKTGVRPERIVRLDEKDNFWS
                     MGDEGPCGPCSEILYDLGENVGCRRPTCAVGCDCDRFLEIWNLVFMEFDRSADGTMTK
                     LPKPSIDTGMGLERVTSVMQGKIGNYDTDLFVPIIRRIEDIAGCAYGESEKNDIAIRV
                     IADHARGATFIINDGILPSKEGRGYVLRRIIRRALRYGRKLGIQKEFLHDLSKSVVDI
                     MEDAYPDVKNNHSYIVRVIRGEEERFIETLGMGMKLYEEFTGDLKKKGSTVIPGELVY
                     KLYDTYGFPVDITTDMALEDGLVLDREGFEKALSEQKERSKTGSKIKGEEWNEGHLTI
                     LGRGLTGTFTGYGTLKDEGVIGAILVGNDTVNEISEGEEGELFLDTTPFYAESGGQVD
                     DSGTITLAGGGATARVTAVTKVKEDLFAHRVVVEKGSFHGGDRVSLAVDEEKRKGTSR
                     NHTATHLLQYALRRVLGDHVKQSGSLVEAGRMRFDFTHFEAMKEEQIRAVEDIVNEKI
                     MECVPVVVNVKSREDAIREGATALFEEKYGETVRVITIGDFSRELCGGTHVANTGQIG
                     SLYILGESSLASGVRRIEATTGRGALTYKRRMEGTLKAIAKRTNTEFDRVEDRVESLL
                     GEIALKEKELDRFKQDIIAHRVDGAIAEAIDREGMKIVTLFVENASADDLRKVTDVVR
                     SKVKDCVVVVGTTGDEDKGLVVASVSKDLQKRYNAGKIIKALTAQYGGKGGGGPNIAQ
                     GGIPGAKVPESLKKVEQFIDT"
     gene            6582..7253
                     /locus_tag="PKN85_00720"
     CDS             6582..7253
                     /locus_tag="PKN85_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT42955.1"
                     /translation="MKRYADNLKSVNLFADLKDTELETISRILYVNTYHRGQLIFQEG
                     EDGNALFVVLKGRVKVCLYDEEGREYVLDVIGKDGFFGELALIDELPRSANAIAMEGS
                     DLLIVRRADFTKLLMENPSISIHILKVLAGRLRVADERIKWLAFLNVEGRILKYLLEV
                     GARLGIKMKDYVIIERGPSQIEIANSCGCSRETVSRMISSLVRKGVISVRRRQYTLYP
                     GTRTF"
     gene            7616..8845
                     /locus_tag="PKN85_00725"
     CDS             7616..8845
                     /locus_tag="PKN85_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HNT42956.1"
                     /translation="MASTVSQKLEAKEQELKILHEVAKDISDNLDLSELLHRIVGTVR
                     DFVKADSCLVYLYDEDNDELVLSASSDPKAESVGSISLKLGEGVTGWAAKEKQPVYLA
                     KEAYRDKRFKAFTSLKEDKYEALLSIPILAKNRIVGVMNLQNKKEHVYPEAQIKLLFT
                     IGRYLGSAIQNAITYDEVVKKAKQLDLLSEVSRTIVSDHYIKEILHLIVTMTAKVMDS
                     KICSVMLLDEKKEELVIAATQSLSNEYVNKPNLKVGQSISGKVVLEKRPLNVLDVTKE
                     PGYMFPDVARKEGFVSLLSVPMMIKDQVVGVINSYTTKEHVFTKEEIDILQAVANQAA
                     VAIENTNLSHEILAAKEALESRKLVERAKGILMRELGLNEDEAYRKIHKKSMDMRKTM
                     KEVAEAIILAFDIQKRT"
BASE COUNT         2022 a         2485 c         2799 g         1568 t
ORIGIN      
        1 cacggcaata tcgggaatga cacgtctgcc cgtcatagcg agcggcggcg tgtcgaagat
       61 agacgacgtc aggcgcctcg cggagatgga cgtctgggcg gcgatcatag ggaaggcctt
      121 ctacgagggg ttcataggca tcgaggaggc catgggttat gctgactaaa aggatcattc
      181 cctgccttga cgtcatggag ggcagggtcg taaaagggac caattttctc gagctcaaag
      241 acgccggcga tcctgtggag aacgcaaagg catacgagga gcagctggcg gacgagctgt
      301 gctttctcga catcaccgct tcccatgaga aaagacggac catcatcgac gtcgtcgagc
      361 gggtctccca cgaggtcttc atgccgctga cggtgggagg cggcatacgc tccatagacg
      421 atatccggga catcctgcgg gccggggcgg acaaggtgac cgtcaacacg acggcggtgg
      481 agaaccccga attcgtcagg gaatcgagcg ggatcttcgg gagccagtgc atatgcgtag
      541 ccatcgatgc caagaggcgt gaaggcggag ggttcgaggt ctacacctat gggggccgca
      601 ggccgacagg ggtcgacgcc atcggctggg cgaagagggt ggaggagctg ggtgccggcg
      661 agatactcct taccagcatg gaccgtgacg ggaccaggat cggtttcgac atcgaattga
      721 cgcggaccat tgccgagtcc gtgaacattc ccgtcatcgc ctccggcgga gtggggaccc
      781 tggagcacct ctatgaaggg ctcgccacgg caaaggcgga cgccgttctc gccgcgtcga
      841 tattccacta ccgggagcac acggtcgtcg acgcgaaacg ctacctgagg gaccgcggcg
      901 tgaacgtgag gctgtagccc cggcagcaga atattggtcc aaaaaccgat catgataagt
      961 aataggagaa agaacgagga gtcgccatgg atgatctgaa atgggatgaa aagggtctta
     1021 tgcccgcggt cgtgcaggat gcaagctcaa aggacgtcct catggtcgcc tacatgaaca
     1081 gggaggccct taagctcacg ctgaagacga agaccgccca ttactattcg aggtcgcggc
     1141 agaagctctg gctcaagggc gagacctcgg gccacacaca ggccgtgaag gaggtccgca
     1201 tcgattgcga caacgatacc atccttcttc tcgtggacca gaaaggcgcg gcctgccaca
     1261 ccgggtactg gagctgcttc taccgcgcct ggtcagatgg atggaagatc gtcggaaaga
     1321 aggtctttga cgaaaaagaa gtctatggtg aaaagaagac ccattgatgc cgcccttttc
     1381 ttcttcaaca cctgttgttt cgtggggtgc atccccggtg cgccggggac ctacgcgtcg
     1441 cttctcgccg cggtactgat ctggctcttc ccggccatct tcggcgatgc cgtcttcgcc
     1501 gtcattctga tcgtcttcgc cgtggcaacg gtcacgatgg agagattcga gggtgaagac
     1561 cccggccgca tcgtcatcga tgagttcgcc ggcatgtgcg tcgccatggc ggggcacagg
     1621 gcgacgctcc tcaccgtggc catcggcttc gttctcttcc gcatcttcga catcctcaag
     1681 ccctttcccg tcgggcgtat ggagagactc aaagggggct ggggcgtagt gggggacgat
     1741 gtggtcgccg gcatattcgc gaacatcctt cttctcctgt ggacgaggtt catatgaaga
     1801 tagaggagag attggtcagg accctgacgg cgagatccct ttccttatgc accgccgaat
     1861 cctgcacggg agggctcatc gcgggccgga taaccgccgt gcccggagcg tcggcgtgct
     1921 tcgaaggcgg ggtggtgacc tacagcaaca gggcgaaggc gatccttctc ggcgttccgg
     1981 ttgaggtcat agaggaccac ggggccgtga gcgaagaggt cgcccgggcg atggctgaag
     2041 gggcgaggga aaggctcggg accgatatcg ccgttgccgt cacgggaata gccgggcccg
     2101 ccggcggctc accggagaag cccgtcggga cggtcttcat ctcgctggcg gtggcgggga
     2161 tgacgtccgt ccgcgggttc cgtttcgagg gcacgagacg cgagatacgc cggcgctctg
     2221 gagacgaggt tctccatttc gtgctcgacc acctcgaggg gaaggtgaac ggatgaggtc
     2281 ctttcttgcc tttgagatcc ccccggaggt caaggcatat ctctcgcagg tgtccagggc
     2341 catggcgaag accctttcgg gcgtaaggtg ggtcaggccc gaggggcaac acatcaccat
     2401 caggttcttc ggggagatat cggaggcgcg ggcgcgggag attgaggaca ttgtcagtgc
     2461 ggccgggccc tacccggggg ttggagcgac cctgaagcgc atcgacgcct ttcccgacaa
     2521 gcgaaggccc cgggtgatcg tcgccaccat cgatgaagga gttgacatca tacgctcgat
     2581 ataccatgat atagagaacc gacttacagc cctgggttac gaacgagaga agcggggttt
     2641 cacgcctcat atcactttcg ggagagtaaa agtgcccgcg cctcttctgg agagagacac
     2701 ccctcccctg gaatgtccac ggttcgcgat cgagcgtatc attctataca agagcatcct
     2761 cggaagggaa ggcgcgacat acgagccgct tttcgaaaag aaactgggtg gcgcggcttg
     2821 aactcagccg cggggaagaa gggaaagaga cccgatgcag gggagagata aaggagaaag
     2881 atgaacccga aaacgctcaa gggaggcatc gacgtgaaag acgacgataa cagaggcaaa
     2941 gccatagaca tggccgtatc tcagatagag aagcttttcg gcaaaggcgc catcatgaag
     3001 ctcggggcga aggcccttga ggccgttccc gtcatctcca ccggctccat cgcccttgat
     3061 ctcgctcttg gcatcggcgg catcccccgg ggcagggtgg tggagatctt cgggcccgag
     3121 gcgtcgggca agacgacact ggcgctccag gtcgtcgccg agatccagaa gacggggggc
     3181 agcgccgcgt tcatcgatgc cgagcatgcc ctggacgtca gctacgcgaa aaggatcggt
     3241 gtcaacaccg acgacctcct catatcgcag cccgataccg gtgagcaggc cctcgagatc
     3301 agcgagatac tcgtgcgcag cggaggtgtc gacgtggtcg ttgtcgactc cgtcgccgcc
     3361 ctggtgcccc gggccgagat cgagggggag atgggcgatt cccacatggg ccttcaggcg
     3421 cggctcatgt cgcaggccct caggaagctc acggcgatca ccagcaagtc catgacaacc
     3481 gtcattttca taaaccagat ccgtcacaag atcgggatca tgttcgggaa gcccgagacc
     3541 acgacgggcg ggaatgccct caagttctat gcctcgctgc gcctcgacat ccgcaggatc
     3601 gccgcgataa aggacggcca ggaggtcatc ggatcgagaa cgagggtgaa gatcgtaaag
     3661 aacaagctcg cgccgccatt cagggaagtc gagtttgaca tcatattcgg cgaaggcata
     3721 tcccgcgagg gcgatgtcgt cgacctcgca gtggagatgg gcattgtcga gaaagcgggg
     3781 acatggtact cctacgggga ttcaaggatc ggtcaaggcc gtgagaacgc caaggagtac
     3841 ctgaagacac accccgaaac gatgaaggaa gtcgaaggaa agataatgat acaggcgggg
     3901 ttgaagaagg gagagacggg tgacatcgca acagatcaga aataaattcc tcgagtattt
     3961 tgcacgcaac ggccacacga tagtacccag ttccgggctc ctgccggaga aagatccgac
     4021 gctccttttc gtgaacgccg ggatggtgca gttcaagaac ctcttcctcg gcctcgaaaa
     4081 gaggccctac gtgcggggga cgtcgtgcca gaaatgcgtg agggccggcg gcaagcacaa
     4141 cgacctcgac aatatcggca agaccctgag gcatcacacc ttcttcgaga tgctgggcaa
     4201 cttctccttc ggcgactatt tcaagaagga ggccatcgcc ttcgcgtggg aattcctgac
     4261 gaaggaactg ggtctcgacg cgtcgaagat gtgggtgacc gtctttcgcg aggacgacga
     4321 ggcggatgaa ctctggagaa agacgggtgt caggccggaa aggatagtgc gactcgacga
     4381 gaaggacaac ttctggtcca tgggagatga agggccctgc ggcccctgct cggagatact
     4441 ctacgatctg ggcgagaacg tggggtgccg ccggccgacg tgcgccgtcg ggtgtgactg
     4501 cgaccggttc ctcgagatct ggaacctcgt cttcatggag ttcgaccgca gcgccgacgg
     4561 caccatgacg aagctgccga agccctccat cgacaccggg atggggctcg agcgtgtcac
     4621 gtccgtcatg cagggcaaga tcgggaacta cgacacggac ctcttcgtcc ccatcatcag
     4681 gcgcatcgag gacatagcgg gctgcgccta cggcgaatcg gagaagaacg acatcgccat
     4741 ccgcgtcatc gccgaccacg cgcggggcgc caccttcatc atcaacgacg gcatccttcc
     4801 ctcgaaggag ggcaggggct atgtgctccg gaggatcatc agaagggcgc tgcgctacgg
     4861 caggaagctg ggcatacaga aggagttcct tcacgatctt tccaagagcg tcgtggatat
     4921 catggaagac gcgtatcccg atgtgaagaa caaccattcc tacatcgtga gggtcatacg
     4981 gggtgaggaa gagcgcttca tcgagaccct gggcatgggg atgaagctct atgaagagtt
     5041 caccggcgat ctgaagaaga agggcagcac cgtcatcccc ggggagcttg tctacaagct
     5101 ctacgatacc tacggcttcc ccgtcgatat aacgacggat atggccctgg aagacggcct
     5161 cgtcctcgac agggagggat tcgagaaggc cctctcggag cagaaggagc gctccaagac
     5221 gggctcgaag atcaagggcg aggaatggaa cgagggccac ctcaccatac tcggccgggg
     5281 tctcaccggc accttcaccg ggtacggcac cctgaaggac gaaggcgtca tcggggccat
     5341 cctcgtcggc aacgacacgg taaatgagat atccgaaggg gaagagggcg agctttttct
     5401 cgacacgacg cccttctacg ccgaaagcgg cggccaggtc gacgactcgg ggacgatcac
     5461 ccttgccggc ggcggcgcca cggcgcgggt gacggcggtg accaaggtaa aggaagacct
     5521 ctttgcccac cgcgtcgtcg tcgaaaaggg gtcgttccac gggggagaca gggtatctct
     5581 cgccgtcgat gaagagaaac gcaagggcac gtcgcgcaat cacacggcga cgcacctcct
     5641 ccagtatgca ttgagaaggg tcctcggcga ccacgtcaag cagtcgggct ccctcgtcga
     5701 ggctggcagg atgcgcttcg acttcaccca cttcgaggca atgaaggagg agcagatacg
     5761 ggcggtggag gacatcgtca acgagaagat catggaatgc gtgcccgtcg tcgtcaacgt
     5821 gaagagccgt gaggacgcca tacgggaggg agcgacggcg ctgttcgagg agaaatacgg
     5881 cgagacggtg cgggtcatca ccatcggcga tttttcccgg gaactctgcg gcggcacgca
     5941 cgtggccaat acgggccaga tcggcagcct ctacatcctc ggtgagagct ctctcgcatc
     6001 aggcgtccgc aggatcgagg cgacaacggg ccggggggcc cttacgtaca agcgcaggat
     6061 ggaggggacc ctgaaggcca tcgcgaaacg gacgaacacc gagttcgacc gtgtcgagga
     6121 ccgtgtcgag agcctcctcg gggagatcgc cctcaaggaa aaggagctcg accgcttcaa
     6181 gcaggacatc attgcccaca gggtcgacgg cgccatcgcg gaagcgatcg acagagaggg
     6241 gatgaagatc gtcaccctct tcgtggagaa cgcctccgcc gacgacctga ggaaggtgac
     6301 agatgtcgtg cggtccaagg tgaaggactg cgttgtcgtc gtcgggacga caggggacga
     6361 ggacaagggg ctcgtcgttg cctctgtcag caaggacctg cagaagcgct acaacgcggg
     6421 gaagatcatc aaggccctta ccgcgcagta cggcggaaag ggcggcgggg gacccaatat
     6481 cgcgcagggc ggcataccgg gggcaaaggt cccggaatcc ctcaaaaagg ttgaacaatt
     6541 catcgacacc tagtatcatg tgaaaaaacg acgggggaga gatgaagcgg tacgcggaca
     6601 atcttaagag cgtcaacctc tttgcggatc tcaaggacac agaactggag acgatctccc
     6661 ggatcctcta cgtcaacacc tatcatcggg gccagcttat cttccaggag ggagaggatg
     6721 gcaatgccct cttcgttgtc ctgaaagggc gcgtgaaggt ctgcctctac gatgaggagg
     6781 gacgggaata cgtcctcgat gtcataggaa aggacggttt cttcggtgaa ctcgccctca
     6841 tcgacgaatt gccccgttcg gcgaacgcca ttgccatgga ggggtcggac ctgcttatcg
     6901 tgcgcagggc ggacttcacg aagctcctca tggagaaccc ctccatttcg atccacatac
     6961 tgaaggtcct cgcgggaagg ctgcgcgttg ccgatgaaag gatcaagtgg ctcgccttcc
     7021 tgaacgtcga aggccgcatc ctcaagtacc tccttgaggt cggggcgcgc cttggcatca
     7081 agatgaagga ctatgtcatc atcgagagag ggccttcaca gatagagata gccaactcct
     7141 gcggctgctc ccgggaaacg gtctcccgca tgatctcgtc gctggtgagg aagggcgtga
     7201 taagcgtgag gagaaggcag tatacgctat acccgggcac tcgcaccttc taggtcctgc
     7261 ttcatatcgg gtcgctgcct tcgttgcctt cgtcgggact gtcctcgacg tacggtcgta
     7321 cgcctccggg agcccctcct cgtcgcctcg gcatcatccc cgatctgaag caggacagag
     7381 aagatccttc catcctccgg ggcgctgcct tcgttgcctt cgtcgggact gtcctcgacg
     7441 tacggtcgta cgcctccggg agcccctcct cgtcgcctcg gcatcatccc cgatctgaag
     7501 caggacagac gggatcgttt accattcttc gaccacgtgt cttgttatct ccctctttta
     7561 ttgacaacgg ccgtcttctc ctctaagata acatctaaaa aaaggctatc cattaatggc
     7621 ttccacggtc agtcagaaac ttgaggcgaa ggagcaggag ctgaagatcc tccacgaggt
     7681 ggcgaaggat atcagcgaca acctcgacct ctcggagctt ttgcaccgca tcgtcggtac
     7741 ggtcagggac tttgtcaagg ccgattcctg ccttgtctac ctctatgacg aagacaatga
     7801 cgaactcgtc ctttccgcgt cgagcgaccc gaaggcggaa tcggtggggt cgataagcct
     7861 gaagctgggc gaaggtgtca ccggctgggc ggccaaggag aagcagcccg tctacctggc
     7921 aaaggaggca taccgcgaca agcggttcaa ggccttcacc tccctcaagg aggacaaata
     7981 cgaagcgctc ctttccatac ccatcctcgc gaagaacagg atcgtgggtg tcatgaacct
     8041 ccagaacaag aaggagcatg tctatccaga ggcccagata aagctcctct tcaccatcgg
     8101 caggtacctc ggcagcgcga tacagaacgc catcacctac gacgaggtag tgaagaaggc
     8161 aaagcagctc gaccttctct ccgaggtctc ccggacgatc gtctccgacc actatatcaa
     8221 ggagatcctc catctcatcg tgaccatgac ggcaaaggtc atggattcga agatctgttc
     8281 cgtcatgctc ctcgacgaga aaaaggagga gcttgtcatt gccgccacac agagcctgag
     8341 caacgagtac gtgaacaagc cgaacctgaa ggtcggacag tccataagcg gcaaggtggt
     8401 cctggagaag agacccctca acgtcctcga cgtgacgaag gaaccgggat acatgttccc
     8461 cgatgtggcg aggaaggagg gcttcgtttc cctcctgtcc gttccgatga tgatcaagga
     8521 ccaggtcgtg ggcgtcatca acagctacac gacgaaggag catgtcttca cgaaggagga
     8581 gatcgacata cttcaggcgg tggcgaacca ggcggccgtt gccatcgaga acacgaacct
     8641 cagccacgag atacttgccg ccaaggaggc tctggaatcg cgcaagctcg tcgagcgcgc
     8701 caagggcata ctgatgcggg agctgggact caacgaggac gaggcctacc ggaagatcca
     8761 caagaagagc atggatatgc gaaagaccat gaaggaagtg gcggaggcga tcatccttgc
     8821 cttcgatatc cagaaaagaa cttgacaaca gtgtgcccgc acactatcat attg
//
LOCUS       DAOQTR010000014         8654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9039_length_8654_cov_3.191883, whole genome shotgun sequence.
ACCESSION   DAOQTR010000014 DAOQTR010000000
VERSION     DAOQTR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9039_length_8654_cov_3.191883"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..3251)
                     /locus_tag="PKN85_00730"
     CDS             complement(204..3251)
                     /locus_tag="PKN85_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT42957.1"
                     /translation="MNDYDPEKEQVVSSGGDDGVRVFFDTPPDAAFIMDSQGTLITLN
                     ENLARRFNHDVDEMVGSNIFDYFSPDVARSRKALMERIITGRRPLSCTDFRDGVWLES
                     CYYPVLDRSGSVAKIAVFSRDVTAVRRAEEALRESEERYRTAIENSNDGVAILKGNIH
                     LYVNGKFVEIFGYDSPADIIGKPQSLTVHPDDYRRVVDFTQRRQRGEIIPERYEFKGV
                     KRNGDLVYIEVSATGTTYRGENVALVYMRDVTARRTSEEELWLARFSIEHASESIFWI
                     RPDGRFVYVNEAACNKLGYRKEELLALRVVDVDPDHTKRELSAIGRCMAEEGSITFRS
                     HHQAKNGRTFPVEVIANHVAYSGEDFIFCFARDVSDKELAEERLNAERQKFQILIEHA
                     PFGMAMFDREDRYLYVNPKFTEIFGYTLAEIPDRASWFERAYPDGAVRESVTDAWHHD
                     DGTKDVGEKMPRTFPVVCKDGATKLINFITVRLENGDYIVSFEDITERRLAEQALANE
                     KERLAVTLRSIGDGVIATDSDGRIVLMNAVAEELTGWKQEEATGTDLVDVFHIINERT
                     RARCESPVDKVLRMGTVVGLANHTALISKDGRELVIADSGAPIWDRDGNIAGVVLVFR
                     DVTEKKRMDEELQKMSKLESVGILAGGIAHDFNNILAAILGNVSLARMYAEPADERVL
                     KRLEDAEQAVLRAKDLTLQLLTFSKGGSPILKTTSIENVVRETASFALTGSGVRCDFM
                     FAEDLFPVDIDEGQMSQVINNLVINSQQAMSGGGVITIEAVNLAAPDEMVEHGVLLQP
                     GSYVRISVKDRGTGIAPEHVHKVFDPYFTTKDKGSGLGLATSYSIIKNHDGHITLDSE
                     PGSGTTFFIYLKASRRLAPSVVRNVRDNRITTVRARILHMDDEAMILKTTKEMLEGLG
                     YDVVCAADGREAIARYREAAAAGTPFDVVLLDITVPGGLGGKGTMRELVAIDPGVRVI
                     VSSGYSNDPVLADHRKYGFKGVLVKPCLMAELDEIIRRVLEEN"
     gene            3497..4348
                     /gene="htpX"
                     /locus_tag="PKN85_00735"
     CDS             3497..4348
                     /gene="htpX"
                     /locus_tag="PKN85_00735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNT42958.1"
                     /translation="MNRLKTFFLMTVLTIILVGLGSIIGGRSGGMIAFAVALVMNFVS
                     YWFSDKIVLKMYGATEVSPQEAPQLHSTVASLAQRASIPMPRVFIIEDDSPNAFATGR
                     NPEHGVVAVTTGIMRILSRDELEGVLAHELSHIKHRDILIQSVAATLAGAITMIANWA
                     RFAAFFGGSSDDDDGGGNIFTVIIFSVIAAFAAMLIQLAISRSREYLADDGGANLSGN
                     PLFLAGALRKLNAGVARNPMNDANPSTAPLFIVNPFSAGGIRSLFSTHPPIEERIKRL
                     EDMAYRR"
     gene            4377..5603
                     /locus_tag="PKN85_00740"
     CDS             4377..5603
                     /locus_tag="PKN85_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNT42959.1"
                     /translation="MRIIRDVESGRFLDESIDSAFSRSRLHEGERALIYQITSGVIRW
                     RGYLEIILSRYAKKPVEKDVRYLLWMTLYQVGFMKKAHYHVVKEAVEYARNEKGKFVA
                     GFVNAVLRRFVSDREDRSLSSAGAVRHAFPKWLVDRWTLRFGASETDGLLELLNREPE
                     FALRVHTLRLTVDRALEALTDGGLEVRRGRLSPSALTVDRLAPVLASSLFREGLVSVQ
                     GEASQLAGMAVAAAGGTTILDACTGSATKTKQVREMCPQARVISMDNKMKRLLLSSLG
                     DHIVCADALAPPFRAGYFDTILVDAPCSSLGIVRKHPEIKWRRSEEDISRFAAMQLSL
                     LRSLWDLVRPGGHVVYSVCSFEPEETTDVIRNLAKDKNFVLENPLPFLFNKDCFLSLP
                     HETALDGFFIARIRKL"
     gene            5600..6295
                     /locus_tag="PKN85_00745"
     CDS             5600..6295
                     /locus_tag="PKN85_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNT42960.1"
                     /translation="MKLLKILFYVVAPVLVFGLSTYLTISLLLKFQQTTVCPDVRGKT
                     VEDARELLRKKDLALVVLRYERRNDVPYNHITVQKPDANISTKKGRVVYVIVSEGPEL
                     MKTPGFVGLTLEETQPVFSDKHLVLEKAVLVPHSKAGKVISQLPAEGTDILEYGKVTL
                     FVGTTPRSYYLMADTRNINYNELADELEAKGIKYRINYARTDQAPARGGIEYSILPRT
                     IFSSTEEIVINIY"
     gene            6309..6971
                     /gene="rpe"
                     /locus_tag="PKN85_00750"
     CDS             6309..6971
                     /gene="rpe"
                     /locus_tag="PKN85_00750"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNT42961.1"
                     /translation="MKDILIAPSILSCDFLRLGQEIRDVEEAGADLIHVDVMDGHFVP
                     NITIGPMFVEALRRINRKPLDVHLMIENPAQYVKQFAEAGSDIVTIHAEADNHLFRTL
                     DVIKESGARAGISFNPATPLTLLRHVINIVDLVLIMTVNPGFGGQKYIPEMAGKIVEA
                     RKMIDKAGRYVDLEVDGGIKAENAAEVVKAGGNVLVMGTEIFHSGDYRKKIEEVHGRL
                     GR"
     gene            6974..7618
                     /locus_tag="PKN85_00755"
     CDS             6974..7618
                     /locus_tag="PKN85_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HNT42962.1"
                     /translation="MELKRQKTYKGVFDRARDELLGSAMEEKFFLAGLGHDRVDSGTR
                     VHVPFFDETILFNIPGFTFSSSRGANVTLVAKIVILHYINTASGTPLTGDKVAYGDIP
                     ACMHYDPVFEKRVLKPLVRAFGYDRHTFLDAGRNLGAKEEGFGDASFTLFAFPKVPIT
                     FVLWEGDSEFPPSARALFDPSVTGYLPLEDIVVVSKLAAGRILKTAMKNFAEEV"
     gene            7620..>8654
                     /locus_tag="PKN85_00760"
     CDS             7620..>8654
                     /locus_tag="PKN85_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT42963.1"
                     /translation="MIFRQFNFDGCLAYVIACERERKGAIIDPSHEMDPFLDFAASYG
                     LAITHIIDTHTHVDHVSLAPELADRLGAKTVMSRNTPAQRELGAKVTDLFGIEKVLAE
                     NALKRVDLYLGDGDELKIGDITLQAMETKGHTRDHMAILTGDRIFTGDVLIIGQCGRT
                     DLPGGSPRDMHESLFGRIMPLSDDLIVYPAHDYKGNINSSLGYEKVNNVCLKTERTVE
                     EFEAFLKGLFPPLDATTGKLQCGLSTMEPAPEGELGTLMKSFCISMEAFLATPHEETV
                     IEPEELLQKIVQKERLFLVDVREPKELQQTGFIEGAVNIPVREVAMRIGEFPKDLDTP
                     VVVYCASGHRS"
BASE COUNT         1803 a         2636 c         2414 g         1801 t
ORIGIN      
        1 catatgcgag aacaaatgcc cgcttgaagg ccgctcggcc gtggaggtct tcgcgaggaa
       61 aaaaggctga accccaaccg gcgggggttt gaccgcttga acagcttgaa cgttgaagag
      121 gaaagagccg tcctttctgc atggggttgt acttgccctg acgctcaagc ggttcacgct
      181 gttcaagcgg ctcaagcgat ccttcagttt tcttccagca cccgtcttat gatctcgtcc
      241 agttccgcca tgaggcaggg tttgacgagg acccctttga aaccgtactt ccgatggtct
      301 gccagaacgg ggtcgttgga atagccgctg gagactatga ccctgacccc ggggtctatg
      361 gcgaccagtt ccctcatcgt gccctttccc cccaaccctc cggggacggt gatgtcgaga
      421 aggacgacat caaagggagt acccgcggcg gcggcttcac ggtacctggc gatcgcctcc
      481 cggccatccg ccgcgcagac cacatcgtaa ccgaggccct ccagcatctc cttcgtggtc
      541 ttgagtatca tcgcctcgtc gtccatgtgg aggatccttg ctctgaccgt ggtgatcctg
      601 ttgtcacgga cattccttac gacggaaggg gcgagccgcc gggacgcctt aaggtagata
      661 aagaaggtcg ttcccgaacc gggttcggaa tcaagcgtga tatggccgtc gtggttcttg
      721 atgatggaat aggaggtcgc gagtccgaga ccgctgccct tgtccttcgt cgtaaagtag
      781 ggatcgaata ccttgtgcac gtgttccggg gctatccccg taccgcggtc tttcaccgat
      841 atccggacat agctgccggg ctgaagtaaa acaccgtgct ccaccatttc atcgggggca
      901 gcaaggttca ccgcctcgat cgtgatcacc ccgccaccgg acatggcctg ttgggagttg
      961 atgacgagat tatttatgac ctggctcatt tgaccctcgt ctatatccac ggggaaaagg
     1021 tcttcggcaa acatgaagtc gcacctgacc ccggagcccg tgagagcgaa actggcggtt
     1081 tccctgacca cattttcgat tgaggtcgtc ttcaggatgg gagaaccgcc cttcgagaag
     1141 gtcagaagct ggagggtcag gtccttggcc ctcaataccg cctgttcggc gtcctcgaga
     1201 cgtttcagca ccctctcatc tgcaggttcc gcgtacattc ttgccagaga cacgttcccc
     1261 agtatagccg cgaggatgtt gttgaaatcg tgagctattc cccccgccag gatacccact
     1321 gactcaagct tgctcatctt ctgcagttcc tcgtccattc tcttcttttc cgtcacgtca
     1381 cgaaagacaa gcaccacacc ggcgatattc ccgtccctgt cccatatcgg ggcgccgctg
     1441 tcggctatga caagttcccg cccgtccttc gagatgaggg ccgtgtggtt tgcgaggcct
     1501 acgaccgtcc ccatccgaag taccttgtcg acgggacttt cgcatcgtgc cctagtcctc
     1561 tcgttgatga tgtggaaaac atcgacaaga tccgttcctg tggcctcctc ctgtttccat
     1621 cccgtcagtt cttcagcaac ggcattcata agaacgatcc ttccgtcgga gtcggtggcg
     1681 atcacgccat ctccgatgga acgcagcgtg accgcgagtc gttccttttc attcgccagt
     1741 gcctgttcgg caagacggcg ctccgttatg tcctcgaagg agactatgta gtcgccgttc
     1801 tcgaggcgga cggtgatgaa attgatgagc tttgtcgccc cgtccttgca caccaccgga
     1861 aaggtcctcg gcattttctc cccgacatcc ttggtgccgt catcatgatg ccaggcgtcg
     1921 gtcacgctct ccctcacggc cccgtcggga taggcacgct cgaaccatga ggcccggtcc
     1981 ggtatctcgg caagcgtgta cccgaaaatc tccgtgaact ttgggttcac atagagatat
     2041 ctgtcctccc tgtcgaacat tgccatgccg aacggggcgt gttctatgag gatctggaac
     2101 ttctgccgct ccgcgttcaa tctttcctcg gccagttcct tgtcggagac gtcccgggcg
     2161 aaacaaaaga tgaaatcctc cccgctgtaa gcaacatgat tcgcgatgac ctcgacgggg
     2221 aaggtgcggc cattcttggc ctggtgatgg gacctgaaag tgatggagcc ctcctccgcc
     2281 atgcacctgc ctatggcgga gagttccctc ttcgtgtggt cgggatcgac atctacgacc
     2341 cgcaaagcga gaagctcctc cttcctgtac ccaagcttgt tgcacgcggc ctcgttcacg
     2401 tacacgaacc tgccgtcggg tctgatccag aagatcgatt ccgaggcgtg ttcgatggag
     2461 aaccgcgcga gccagagctc ttcctcgctc gtccgcctgg ccgtcacgtc cctcatgtac
     2521 accagggcga cgttctcccc cctgtaggtg gtccccgtcg cggaaacctc tatatagacg
     2581 aggtcgccgt tgcgcttgac ccccttgaac tcataccgct ccggtattat ctcgccgcgc
     2641 tgcctgcgct gtgtgaaatc gaccaccctc cggtagtcgt cgggatggac ggtgagggat
     2701 tgcggtttcc cgatgatgtc ggcggggctg tcatagccga agatctcgac gaacttcccg
     2761 ttcacgtaaa gatggatgtt ccctttcagt attgccacgc cgtcgttgga attttcgatg
     2821 gccgtccggt accgctcctc cgactccctc agggcctcct cggcccggcg gacggccgtc
     2881 acatcgcgac tgaacaccgc gatcttcgcc accgaccctg accggtcgag aacgggatag
     2941 taacagctct cgagccagac accatcgcgg aaatcggtgc aggaaagggg ccttcgcccc
     3001 gtgattatcc tctccatcag ggccttcctg ctacgggcca catcgggtga gaaataatcg
     3061 aatatgttgc tgcccaccat ttcgtccaca tcgtggttga aacggcgcgc caggttctca
     3121 ttgagcgtaa tgagcgtccc ttggctgtcc atgatgaaag ccgcgtcggg tggagtatcg
     3181 aaaaagacgc ggacaccatc atctcccccg gaggatacaa cctgttcctt ctccggatca
     3241 taatcgttca taggcacctc acaacagggg taccgtgccc caactccaag ggggacggac
     3301 acacccgcct cgttcgtatc tttcctgaat cctgcgcagg tttttatctt ttaagaagcc
     3361 tgttgactga ccccgattcc tggtcattat tctatgaacc ttttgaacca aaaggcaagg
     3421 atttcgaaca gcgccccggc ttgataaaag acataaaatg tttataatgt ataggataaa
     3481 tctttgcggg ggttcgatga acaggctcaa gactttcttt ctcatgacgg tcctcaccat
     3541 tattctggtc ggtcttggca gtatcatagg gggcaggtcc ggaggcatga tcgcattcgc
     3601 cgttgccctc gtcatgaatt tcgtcagcta ctggttctcg gacaagatag tcctcaagat
     3661 gtacggcgcg acagaggttt ctcctcagga ggcaccgcag cttcacagta cggtggcatc
     3721 cctggcgcag cgcgcctcca tcccgatgcc aagggtgttc atcatcgagg acgacagtcc
     3781 caacgccttt gccaccggcc gcaatcccga gcacggggtg gtggcggtaa cgacgggcat
     3841 catgcgcata ctctcgcggg acgaactcga gggtgttctc gcccatgagc tctcccacat
     3901 aaagcaccgc gacatcctca tacagagtgt tgccgcgaca ctggcggggg ccatcaccat
     3961 gatcgccaac tgggcccgtt ttgcggcctt cttcgggggc tcttccgacg atgatgacgg
     4021 gggcggcaat atcttcaccg tcatcatctt ctccgtcatc gcggcctttg ccgcgatgct
     4081 catccagctc gccatctccc gctcccggga gtacctggcc gatgacggcg gagcgaacct
     4141 gtcgggaaat cccctctttc tcgccggggc cctgcgcaag ctcaatgccg gcgtggcgcg
     4201 caatcccatg aacgacgcga acccctccac ggctccgctc ttcatagtga accccttcag
     4261 cgcgggaggc attcgctccc tgttcagcac ccaccctcct atcgaagaga ggatcaagag
     4321 actggaggac atggcctatc ggcgataact tcggacatct cctcagggcc atttcgatcc
     4381 gcatcattcg ggatgttgaa tccggcaggt tcctcgatga atccatcgat tcggccttct
     4441 cgcgctcacg cctgcacgag ggtgagcggg cgctgatata ccagataacc tcgggggtga
     4501 tacggtggcg gggatacctc gagatcatcc tctcccggta cgccaaaaaa cctgtcgaaa
     4561 aggacgtccg atacctcctc tggatgacgc tctaccaggt cgggttcatg aagaaggccc
     4621 attaccatgt cgttaaggag gccgtggaat acgccaggaa tgaaaaagga aagttcgtgg
     4681 ccgggttcgt gaacgccgtg ctgaggaggt tcgtgagcga cagggaggac cgctctctct
     4741 cctctgccgg ggcggtgcgg cacgccttcc cgaaatggct cgtcgaccgc tggacgctga
     4801 ggttcggggc cagtgagacg gacgggctcc ttgagctctt gaacagggaa cccgagttcg
     4861 cgctcagggt gcacacgctc agactgaccg tcgacagggc gctcgaagcc ctgacggatg
     4921 gcgggctcga ggttcgccgt ggccgtcttt cgccctcggc cctcaccgtc gacaggctcg
     4981 ccccggtgct cgccagctcc cttttcaggg aggggctcgt gagcgttcag ggcgaggcgt
     5041 cccagctcgc gggcatggcg gtcgcggcag cgggcggcac cacgatcctc gacgcgtgca
     5101 ccggttcggc gacgaagacg aagcaggtga gggagatgtg tccccaggct cgcgtcatct
     5161 ccatggacaa caagatgaag cgcctccttc tgtcgtcgct cggcgatcat atcgtctgtg
     5221 ccgacgccct tgccccgcct ttcagggccg gatacttcga caccatcctt gtcgacgcgc
     5281 cctgctcatc gctgggcatc gtccgcaagc accccgagat caagtggcgg cggagcgaag
     5341 aagacatctc ccggttcgcc gcgatgcagc tttccctgct caggtctctg tgggacctcg
     5401 tgcgaccggg agggcatgtg gtctacagcg tctgctcctt cgagcccgaa gagacgacgg
     5461 atgtcattcg caacctggca aaagataaga actttgttct tgaaaatccg ctgccgttcc
     5521 tgtttaataa ggattgtttt ctttcgttgc cgcatgagac ggctctggac ggtttcttta
     5581 tagcacggat aaggaaacta tgaagctctt gaagatccta ttctatgttg tggcaccggt
     5641 tcttgtcttc ggtctgtcga catacctgac gataagcctc ctcctcaagt tccagcaaac
     5701 cacggtctgc cccgatgtcc gggggaagac cgttgaggac gcccgggagc ttctcaggaa
     5761 aaaagatctc gccctggtgg tcctcaggta tgaacggcgc aatgatgtgc cttacaacca
     5821 catcaccgtg cagaaacctg acgccaacat atccaccaag aaagggaggg tggtctacgt
     5881 catcgtttcc gaaggaccgg agctcatgaa gaccccgggg ttcgtggggc tcactctcga
     5941 ggagacccag ccggtgttca gcgacaagca cctcgtcctc gaaaaggcgg tcctcgttcc
     6001 ccactccaaa gcggggaagg tcatctccca gctgccggca gaaggcacgg acatcctcga
     6061 atacgggaag gtcacccttt tcgtcggcac gacaccgagg tcctattatc tcatggccga
     6121 cacgcgcaac atcaactaca acgagctcgc cgacgagctg gaggcgaaag ggataaagta
     6181 caggatcaat tacgccagga ccgatcaggc acctgcgcgc ggcggcatcg agtattcgat
     6241 actccccaga acgatattca gttccacgga ggagatcgtc atcaacatct actagccatg
     6301 gaggcattat gaaagacata ctcatcgcgc cgtccatcct ctcctgcgat ttcctgcgtc
     6361 tcggccagga gatccgggat gttgaggaag cgggcgcgga cctcatccac gtggacgtca
     6421 tggacggcca tttcgttccc aacataacca tcggccccat gttcgtcgag gccctgcgca
     6481 ggatcaacag aaaacctctc gatgtccacc tcatgataga gaacccggca caatacgtga
     6541 agcagttcgc cgaggccggt tccgacatcg tcaccataca cgccgaagcg gacaaccatc
     6601 ttttccggac ccttgacgtc atcaaggagt cgggggcgcg ggcgggcata tccttcaatc
     6661 cggcgacacc gctcaccctg ctcaggcacg tcatcaacat cgtcgacctc gttctcatca
     6721 tgaccgtcaa ccccggtttc ggaggccaga agtacatccc cgagatggcc gggaagatcg
     6781 tggaggcgcg caagatgatc gataaggccg gcaggtacgt ggacctcgag gtcgacggcg
     6841 gtataaaggc ggaaaacgcc gccgaggtcg tcaaagcagg cggcaatgtc ctcgtcatgg
     6901 gcacggagat atttcacagc ggagactacc ggaagaagat cgaagaggtg cacggcaggc
     6961 tgggccggta acgatggagc tcaagagaca gaagacgtac aaaggcgtct tcgacagggc
     7021 acgcgacgag ctcctgggaa gcgccatgga ggagaagttc ttcctcgcgg gcctcggcca
     7081 cgaccgggtc gacagcggga cgcgcgtcca tgtccccttt tttgacgaga ccatcctctt
     7141 caacatacct gggttcacct tttcgagttc caggggggcc aatgtcaccc tcgtcgcaaa
     7201 gatcgtcatc ctccactaca taaacaccgc ttcggggacc ccgctcacgg gcgacaaggt
     7261 cgcctacggg gacatccccg catgcatgca ctacgacccg gtcttcgaga aaagggtact
     7321 caaacccctt gtcagggcat tcggctacga caggcacacc tttctcgacg ccggccgcaa
     7381 ccttggggca aaggaggaag ggttcggcga cgcctcgttc acgcttttcg ccttcccgaa
     7441 ggtacccata acctttgttc tctgggaagg ggacagcgag ttcccgccat cggccagggc
     7501 ccttttcgat ccctccgtga cgggttacct gccgctcgag gatatcgtgg tggtatcgaa
     7561 gctagccgcc ggaagaatac tgaagacggc gatgaagaac tttgccgaag aggtgtgaca
     7621 tgatattcag acaattcaat ttcgacggat gcctcgctta tgtcatcgca tgcgaaaggg
     7681 agagaaaggg ggcgatcatc gacccctccc atgaaatgga ccccttcctc gatttcgccg
     7741 catcttacgg gctcgccata acgcatatca tcgataccca cacccacgtc gaccacgttt
     7801 ccctcgcgcc ggagcttgcc gacagacttg gagccaaaac ggttatgagc agaaacacac
     7861 cggcacaaag ggagttgggg gcaaaggtca cggacctctt cgggatcgag aaggttcttg
     7921 ccgagaacgc cctgaaaagg gtcgacctct acctcggtga cggggatgaa ctgaagatag
     7981 gcgatatcac actgcaggcg atggaaacga agggtcacac ccgggaccac atggcgatcc
     8041 tcaccggcga caggatcttc accggtgatg tccttatcat aggtcagtgc ggaaggacgg
     8101 acctgcccgg cggaagtccg cgggacatgc acgaaagcct cttcggcagg atcatgcccc
     8161 tctccgacga cctcatcgtc tacccggccc acgactataa gggcaacatc aattcatcac
     8221 tgggctacga gaaggtgaac aacgtgtgcc tcaagactga gcgcaccgtg gaggaattcg
     8281 aggcgttcct gaaaggtctc ttcccccctc tcgacgcgac aacagggaaa cttcagtgcg
     8341 gcctcagcac catggaaccc gccccggagg gcgagctggg aactctcatg aaaagcttct
     8401 gcatctccat ggaggccttc ctcgcgacac cccatgagga aacggtcatt gagcccgaag
     8461 agttgctgca gaagatcgtg cagaaggaaa ggctcttcct cgtcgatgtc agggaaccga
     8521 aagagctgca acagacaggc tttatcgaag gcgccgtgaa catccctgtt cgtgaggtcg
     8581 ccatgaggat aggcgagttc ccgaaagacc tcgacacccc cgtcgtcgtc tattgcgcaa
     8641 gcggccaccg ctcc
//
LOCUS       DAOQTR010000015         8620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9084_length_8620_cov_3.850204, whole genome shotgun sequence.
ACCESSION   DAOQTR010000015 DAOQTR010000000
VERSION     DAOQTR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9084_length_8620_cov_3.850204"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PKN85_00765"
     CDS             <1..925
                     /locus_tag="PKN85_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT42964.1"
                     /translation="GVDAFVPSIMEHIELAGVHSGDSACAIPPRTLSEEHIATINEYT
                     KKLAIELKVVGLMNIQYAIAEDKVYILEANPRASRTVPLVSKVTGVQMARIATQLMLG
                     RKLKDMKLTQKTYPHVGVKEAVFPFNMFPEVDPTLGPEMKSTGEVLGMANSFGLAFAK
                     SQEAAGQKLPRSGNVLITVVDRDKDGIVDVAKQLAKLGFTIVATEGTHRFLSDNGVKS
                     TLIKKVHEGRPNIVDAIHNKEIHLVINTPSGKSSMYDDSYIRKTAIKYKIPYITTTAA
                     AKATAEGIKASVAKKGEIKVKSLQSYHRAIK"
     gene            complement(1084..1500)
                     /locus_tag="PKN85_00770"
     CDS             complement(1084..1500)
                     /locus_tag="PKN85_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNT42965.1"
                     /translation="MKSYRKELWFNVPARRGFVNITPQVEECLRESGVSEGLVLVNAM
                     HITASVFVNDDESGLHHDFDVWLERLAPHEPVSGYRHNVGEDNADGHLKRQIMGREVV
                     VAITDGRLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(1642..2193)
                     /locus_tag="PKN85_00775"
     CDS             complement(1642..2193)
                     /locus_tag="PKN85_00775"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42966.1"
                     /translation="MRRIVVDIDNTLWDLAPVLCERLQEASPGFPHHSQWHDWSFWKG
                     LIPADRLYGIIRDVHMEQDTFEPYRDSRDFLQRLRGKGFHIVIASHRERETYASTERW
                     LEKHGLPFDEIHLSYDKTVLFDDCWAIVDDSPVTLEKARQAGIVRAGLRNPWNESEEH
                     PLFADLGEIYRYLETQCTAGKRD"
     gene            2377..3519
                     /locus_tag="PKN85_00780"
     CDS             2377..3519
                     /locus_tag="PKN85_00780"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNT42967.1"
                     /translation="MKISQRFLAGQTASPLRGAVAPDETADLVLAFGSKELLNDGDSF
                     GALRALYPAAHLCGCSTAGEICGTGVFDDTLAATVISFEGTRIMGAGITLTAVSNSKE
                     AGALLARSIEMEGLVHVFVLSDGLNVNGSELVEGLSGSLPEKVTITGGLAADADRFEE
                     TYVFWDGPPRQNSLAIVGFYGNHLKVGYGSMGGWDPFGPDRLVTRSSGNVLYEIDGRS
                     VLDLYRRYLGDRARDLPSSGLFFPLCVRTGEGEKGIVRTLLSVNEKEGSMTFAGDIPQ
                     GSYARLMKANFERLIDGASGAAATAREAMGSVPVELAVLISCVGRKLVLHQRVEEEIE
                     ASREVLGPDAVVTGFYSYGEISPFTPGGKCALHNQTMTVTTLSEVR"
     gene            3528..5942
                     /locus_tag="PKN85_00785"
     CDS             3528..5942
                     /locus_tag="PKN85_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT42968.1"
                     /translation="MHGLLRRQLKKAFGEAFSVPPEWKGFIEMVDTAYRESDTDRDML
                     ERSLELSSQELSEMNSEMRAIFEAIPDLLFRVDREGIILDCKTGNTSDLVFARERLIN
                     RKIDDIPVPSVSRQFAEAMDAVRRTKSPANAEYSLNIDGNEQFYEARFIPSAKGQIVI
                     MVRNVTERRRAEKALMESEEKYRSIFENSIEGIFQTTRDGEYVEANPALVHMLGYGSQ
                     EEMLRESGTVTRQYVVPDDRKRFRELIERHDVLQGFETRVYRRDGSRIWISINAHVVR
                     DRKGNIVFYEGTVENITDRKRAEEALFESEEKYRSIVEGSHVGVYIIEDGLFRFVNAT
                     LCDILGYTYGEIADTMGPGDIILPEDFPAVGESLQKRLSGEIKTAELIYRIRRKDGEI
                     RSIKAIGSSILYRGRPAVIGTLLDITKEKELEAQLLQSQKLETVGRLAGGIAHDFNNM
                     LGVILGNVQMAKINLSASEHTYGFCDAIERATIKAADLVRQLLAFSRRQLLELKTFDL
                     NDLVSGFEKMIHRVIAENIDMTFILAKDLPAIKADETQMNQVLLNLVVNARDAMADGG
                     ILTISTGNVDVEAKYVQTHPDVRPGRYVMLSVADTGIGMEKDVFDRMFEPFFTTKAGG
                     SGLGLSVVYGIVKQHGGFVEVMSERGRGTTIQVFLPRAEEAVMPTVKPEMPVERGSET
                     VLIVEDDEDFRKVEAEMLTRLGYRVYAAADGMEGIALFAEKSEEIDLVILDVVMPRMS
                     GRDALEGMRRIRPDIPSLFVTGYSLDGIHVNYILDQDIEALQKPFSFDTLSRKIREVM
                     RNRGRG"
     gene            complement(6008..6259)
                     /locus_tag="PKN85_00790"
     CDS             complement(6008..6259)
                     /locus_tag="PKN85_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42969.1"
                     /translation="MKKALVLFFAVVIGLSFAFTGFAQEKKAAPATPAAPATPAAKVE
                     APAPVEKEKPAKKEKKVKKTKKTKKAKGAPAEEKPAETK"
     gene            complement(6420..6935)
                     /locus_tag="PKN85_00795"
     CDS             complement(6420..6935)
                     /locus_tag="PKN85_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT42970.1"
                     /translation="MKFDFMVKRLSPVLKRITKRLNGHHAYFDEEDLYQEALTHLWGA
                     FRKGSIDDKTDSYILQGCYYHLKNHLRKVRENAVFVSLSEPAGEDGISWEEMIASDES
                     SSFARLEDKLKFQSITDACSSDRDRQVLTLLMEDLSVREVGTRLGISHVMVLKIRNRI
                     RDNYFRRIGEK"
     gene            complement(6932..>8620)
                     /locus_tag="PKN85_00800"
     CDS             complement(6932..>8620)
                     /locus_tag="PKN85_00800"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNT42971.1"
                     /translation="GLCQMNSTVGDMTGNGERVVEWVEKAKAEEVDILALPELVITGY
                     PPEDLLLKKGFVDANIRVITEIASRIDSPATIVGFVDRSRSGIHNAAAVIYKGKIRGV
                     FRKELLPNYGVFDEKRYFLPGSRPRVFRYGKVLFGVVICEDMWFREGPIHVMAQAGAG
                     LIFNINASPYHAGKIYVREDVVRSRTREDRVWVAYGNLVGGQDELVFDGQSFVMDRSG
                     TVVAAAGAFREDLLVVDIPEKELLRKARPVSAAGQVTTVAARPGGLRPSVKKPLTPRP
                     VTRLDVVEEVYEALKLGLADYVRKNGFRGTVIGLSGGIDSALVAALAADALGSDNIVC
                     VFMPSRFTSRESSEDAFDLAKRLGVRIIEVPIDPVFSAYKASLATLFHGLGEDVTEEN
                     LQARIRGNILMALSNKFGWLVLTTGNKSEMSVGYATLYGDMAGGFAVIKDVPKTLVYK
                     LCTWRNGLGAVIPERIISKEPTAELKADQKDRDSLPPYDHLDQVLKSYVEEDTEPSAA
                     DFPNLPAEEIARVLRMVDLSEYKRRQSPPGIKITPKAFGKDRRMPITNKYKGSQ"
BASE COUNT         1823 a         2564 c         2472 g         1761 t
ORIGIN      
        1 cggcgtcgat gccttcgtac cctccatcat ggaacacata gagctcgccg gagtccactc
       61 gggagacagc gcctgcgcca tacccccccg gacactgtcg gaagagcata tcgccacaat
      121 aaacgaatac acgaagaagc tcgccatcga actcaaggtc gtgggtctca tgaacatcca
      181 gtacgccatc gccgaagaca aggtctacat cctcgaggcg aatccccggg cgtcgagaac
      241 ggtaccgctc gtctccaagg tgacgggtgt ccagatggcc cgcatcgcaa cacagctcat
      301 gctgggcagg aagctcaagg acatgaaact cacgcagaag acatatcccc acgtgggcgt
      361 caaggaggcc gtcttcccct tcaacatgtt ccccgaggtc gacccgaccc ttggcccgga
      421 gatgaagtcc accggcgagg tcctgggcat ggccaactcc ttcggcctgg ctttcgcgaa
      481 atcccaggag gccgccggtc agaagctccc ccgctcgggc aacgttctca tcaccgtcgt
      541 cgaccgcgac aaggacggga tcgtcgatgt cgcgaaacag cttgccaaac tgggattcac
      601 catcgtcgcc accgagggga cccaccggtt cctctccgac aacggcgtga aaagcaccct
      661 catcaagaaa gtgcacgagg gccggcccaa catcgtcgac gccatccaca acaaggagat
      721 ccacctcgtc atcaacacgc cctcaggcaa gagcagcatg tatgacgatt cctacatccg
      781 aaagacggcc atcaagtaca agatacccta catcaccaca acagccgccg cgaaggccac
      841 cgccgagggc atcaaggcat ccgtggccaa gaaaggcgag atcaaggtaa aatcactgca
      901 atcctaccac agggcgataa aataaaaaaa acagcccctg cccatccttt gggatgcgca
      961 ggggctgccg cttcttctgg aataggacct ataagaccta caggacgcat gggacttata
     1021 gggttcgtgt ttctttcccg gcgttcaagc gattcaagcc attcaatcgg ctcaagccgt
     1081 ctttcactct cctatgatct tgacgagcac ccgttttctt ctgcggccgt cgaattcgcc
     1141 gtagaatatc tgctcccagg ggccgaagtc gaggcggccg tccgttatgg ccacgaccac
     1201 ctccctgccc atgatctggc gctttaagtg cccgtcggcg ttgtcctcac cgacattgtg
     1261 cctgtagccc gagacgggct cgtggggggc gagcctctcg agccagacat cgaagtcgtg
     1321 atggaggccc gactcgtcat cgttcacgaa gaccgacgcc gtgatatgca tggcgttcac
     1381 gaggacaagg ccctcggaga ccccgctctc acgcaggcat tcctccacct gcggcgtaat
     1441 attcacaaac ccccgccggg cgggcacatt gaaccagagc tcttttctgt agcttttcaa
     1501 gccttcgccc tctctgcgtc ctatggaccc cgtgtctgtc ctgtaggtcc taagcgtcct
     1561 atacgtcgta ttggtcctat aggtcctatt cagaagcgat aggatacttg taggatatag
     1621 gacaccttaa gaaccctcgg gtcagtctct ctttcccgcg gtgcactggg tctcaagata
     1681 ccggtagatc tcccccaggt ccgcgaagag agggtgctct tcggattcgt tccaggggtt
     1741 cctgaggccg gccctgacta ttccggcctg cctggctttt tccagggtca cggggctgtc
     1801 gtcgacgatc gcccagcagt catcgaagag caccgtcttg tcatagctca ggtggatctc
     1861 gtcgaagggc agtccgtgct tctcaagcca cctctccgtg gaggcatagg tctccctctc
     1921 ccgatggctg gcgatgacta tgtgaaagcc ttttccgcga agcctttgga gaaagtcgcg
     1981 ggaatcgcgg tagggttcaa aggtatcctg ctccatgtgg acgtcccgga tgatgccgta
     2041 gagccggtcc gccgggatga gccccttcca gaaactccag tcgtgccact gtgagtggtg
     2101 ggggaacccg ggactggcct cctgcagccg ctcgcagagc actggcgcca ggtcccacag
     2161 ggtattatcg atatcgacga ctatccttcg cacaacgact cattgtacca caaaaagcgt
     2221 gatttccaaa acagggtggg gtatcatcat cgcttgggga agaatgctta agggctctcg
     2281 ccgttccgac gagaaaactt tatgtccggg ggcacgagcc gggtcacacc cgggaacagg
     2341 gatcatgtca tcgggaatac ctgaaggggg aagattatga agatctcgca gaggttcctt
     2401 gccggacaaa cagcttcgcc gctgcgcgga gccgtcgctc ccgatgagac cgcggacctt
     2461 gttctggcct ttggctcgaa ggagcttctc aatgacggcg actccttcgg ggcccttcgg
     2521 gccctgtatc ccgccgccca tctctgtggt tgctccactg ccggcgagat ctgcggtacg
     2581 ggggtcttcg acgatacgct cgccgccacg gttatatcat tcgaagggac gcgcatcatg
     2641 ggggcgggca tcaccctcac ggccgtctcc aacagcaagg aagccggcgc cctgctggcc
     2701 cgttccatcg agatggaggg ccttgtccac gtattcgttc tctccgacgg cctgaacgtc
     2761 aacggcagcg aactcgtcga aggactctcc ggaagcctgc ccgaaaaagt taccatcaca
     2821 ggtggtcttg cggcggacgc ggatcgtttc gaggaaacct atgtgttctg ggacgggcca
     2881 ccccgacaga attcccttgc catcgtcggc ttctacggaa accatctgaa ggtcggctac
     2941 ggctcaatgg gcggctggga ccccttcggt cctgaccgtc tcgttacgag atcctcgggc
     3001 aacgtgcttt acgagataga cggcaggtcg gtgctggacc tctacaggag atatcttggc
     3061 gacagggcgc gcgatctgcc ttcgtcgggc ctattcttcc ctctctgcgt ccggacgggt
     3121 gaaggggaga aaggcatcgt ccgcaccctt ttgtccgtga acgagaagga aggttcgatg
     3181 accttcgccg gcgacattcc gcaaggttca tacgcccggc tgatgaaggc aaactttgaa
     3241 aggctcatag acggtgcctc cggggccgcg gcgaccgccc gcgaggccat gggctccgtc
     3301 cctgtcgaac tcgccgtact catcagctgc gtgggcagaa agctggtttt acaccagcgc
     3361 gtcgaagagg agatcgaggc gtcccgggag gtcctcggcc ctgacgccgt cgtcaccggc
     3421 ttttactcct acggtgagat ctcccccttt acccctggcg ggaaatgcgc tctccacaac
     3481 cagaccatga cggtgacgac cctgtcggag gtacggtagt gacaagaatg cacggcctcc
     3541 tgcgtcggca actgaagaag gcctttggcg aggccttctc ggtgccgccc gaatggaagg
     3601 ggttcatcga aatggtcgac accgcctacc gcgaatcgga cacggacagg gacatgctgg
     3661 aacgctccct ggaactgagt tcccaggagc tctcggagat gaactcggaa atgagggcca
     3721 tctttgaagc gataccggac ctgctcttcc gggtggaccg cgaaggcatc atcctggact
     3781 gcaagacggg aaacacctcg gacctcgtct tcgcccgcga acggctcatc aaccggaaga
     3841 tcgacgacat acccgtccca tccgtaagca ggcaattcgc ggaggccatg gacgccgtga
     3901 ggagaacgaa gtctccggcc aacgccgagt actcgttgaa tatcgatggg aacgagcagt
     3961 tctacgaggc gcgcttcata ccttccgcga agggccagat cgttatcatg gtgcggaacg
     4021 tgacggagcg gcgacgggcg gaaaaggccc tcatggaaag cgaggagaaa tatcgcagca
     4081 tattcgagaa ctccatcgag ggcatattcc agaccacgcg tgacggcgag tatgtcgagg
     4141 caaatcccgc ccttgtccac atgctggggt atggatcaca ggaagagatg ctccgtgagt
     4201 ccggcaccgt gacccgtcag tatgtcgtgc ccgacgatcg gaagcgcttc agggaactca
     4261 tcgaaaggca cgatgtgctt caagggttcg agacccgggt ctatcgcagg gacggcagca
     4321 ggatatggat atccattaat gcccatgtcg tcagggaccg aaaggggaac attgtctttt
     4381 atgaagggac ggtagagaac attaccgaca ggaagcgggc cgaagaggcc ctttttgaat
     4441 cggaagagaa atacaggagc atcgtcgaag ggtcccatgt cggcgtctac atcatcgagg
     4501 acggcctttt tcgcttcgtg aacgcgacac tctgcgatat actcgggtac acctacggcg
     4561 agatagcgga cacgatgggc cccggggaca tcatactgcc agaggacttc cccgccgtcg
     4621 gcgaaagtct ccaaaaacgc ctctcgggcg agatcaagac agcggagttg atctatcgca
     4681 tccgcagaaa ggacggggag atccggtcca taaaggcgat cggaagctcc atcctttaca
     4741 ggggacgccc cgccgtgatc gggaccctcc tggacattac caaggaaaaa gaactcgagg
     4801 cgcagcttct gcaatcccag aagctggaaa cggtgggaag gctcgccggc ggtatcgccc
     4861 acgatttcaa caacatgctc ggggtgatcc tgggaaatgt ccaaatggcg aagatcaatc
     4921 tgtccgccag cgaacacacc tatggtttct gtgacgccat cgaaagggcg actataaaag
     4981 cggccgacct ggtgcgccag ctcctcgcgt tctcaagacg ccagcttctg gaactcaaga
     5041 ccttcgatct caacgacctc gtctcgggct ttgaaaagat gatacaccgt gtcatcgccg
     5101 agaacatcga tatgaccttt atcctggcga aggatctccc ggcgatcaag gccgatgaga
     5161 cacagatgaa ccaggtcctg ctcaatctcg tcgtaaacgc cagggacgcc atggccgacg
     5221 gagggatact caccatctcc acgggtaatg tggatgtcga ggcgaaatat gtccagaccc
     5281 atcccgacgt gcgaccgggc cgctatgtga tgctctccgt cgccgacacc gggattggga
     5341 tggaaaaaga tgttttcgac aggatgttcg aacccttctt caccaccaag gccggtggaa
     5401 gcggtctcgg actttccgtc gtctacggga tcgtcaagca gcacggaggt ttcgtagagg
     5461 tcatgagcga acgcggacgg ggtacgacga tacaggtctt tcttcccagg gcggaggagg
     5521 cggtaatgcc caccgtgaaa cccgagatgc ccgttgagag agggagcgag acggttctca
     5581 tcgttgagga cgatgaggat ttcaggaagg tcgaggcgga aatgctcacg cgcctcggct
     5641 accgcgtgta tgccgcagcc gacggtatgg agggaatagc gctcttcgcc gaaaaaagcg
     5701 aagagataga tctagtcatc cttgacgtcg tgatgccgcg gatgagcggc cgggacgccc
     5761 tggaaggcat gcggaggatc cgccccgaca tcccctcact gttcgtgacg gggtacagcc
     5821 ttgacggcat acacgtgaac tacattctcg atcaggatat cgaggccctt cagaagcctt
     5881 tctccttcga taccctctcc cggaagatac gggaggtgat gcggaacagg ggcaggggat
     5941 gagatgaaaa ggggagaatc tcttctcccc tttcgtgaaa cagttttccg ggtatttgct
     6001 tgcttgccta cttcgtttcg gccggtttct cctcggcggg agctcccttg gccttcttcg
     6061 tcttcttcgt cttttttacc ttcttttcct tcttcgccgg tttctctttc tcaaccgggg
     6121 ccggagcctc taccttggcg gcgggcgtcg cgggggcggc gggcgtcgcg ggggctgcct
     6181 tcttctcctg cgcaaaccct gtaaatgcaa aggaaagacc gatcacgact gcaaagaaca
     6241 gtaccaatgc cttcttcatt ctgtttcacc tccttcctct tgtattggga acattagaga
     6301 gatatataaa ccaagtcgaa aataaaagat agaaaaaaat acaggtgatg ggtcataggt
     6361 catgggtgat gggagagcgt gctttcccgg ggtctttggt ctctgcttga ggcctgaggc
     6421 tacttttccc ctattcttcg aaagtagttg tcgcggatcc tgttgcggat cttgaggacc
     6481 atgacgtggg agatgccgag gcgtgtcccc acctctctca ccgacaggtc ttccatgaga
     6541 agcgtgagga cctggcggtc ccggtccgaa ctgcaggcgt cggtgatcga ctggaacttc
     6601 agtttgtcct cgaggcgcgc gaaggacgag gattcgtcgg aggctatcat ctcctcccag
     6661 gaaatgccgt cctcccccgc cggctcgctg aggctcacaa aaaccgcgtt ctcgcgcact
     6721 ttccggagat ggttcttgag gtgatagtag cagccctgca ggatgtagct gtccgtcttg
     6781 tcgtcgatgg accccttgcg gaaggcgccc caaaggtggg tcagcgcctc ctggtagagg
     6841 tcttcctcgt caaaatacgc gtgatggccg ttcaatctct tcgttatcct cttcaagacc
     6901 ggtgagagcc tttttaccat aaaatcgaat ttcattgcga acccttatac ttgttggtga
     6961 tgggcatcct cctgtccttg ccgaatgcct tgggtgtgat cttgatgccc ggcggggact
     7021 ggcggcgctt gtattcgctt aagtccacca ttctcagaac gcgggcgatc tcctccgccg
     7081 gtaagttcgg aaaatcggcc gcggaaggtt ccgtgtcctc ttcaacgtag cttttcagca
     7141 cctgatccag atggtcgtag ggcggaagac tgtcgcggtc cttctgatcg gccttcagct
     7201 ccgccgtcgg ctctttcgat attatcctct cgggaatgac cgcaccaaga ccgttgcgcc
     7261 atgtgcagag cttgtaaacg agcgtcttcg ggacatcctt gataacggcg aaacccccgg
     7321 ccatatcgcc gtacagggtc gcgtacccca cgctcatctc cgatttgttg cctgtcgtga
     7381 gcacaagcca accgaacttg ttcgagagcg ccatgagaat gttgccgcgg atacgcgcct
     7441 ggaggttctc ctccgtaaca tcctccccga ggccgtggaa gagcgtcgcg aggctcgcct
     7501 tataggcgga gaacacggga tctatgggca cctcgatgat cctcacgccc agtcttttcg
     7561 caaggtcgaa ggcatcttcc gaggactccc gggatgtgaa cctggaaggc atgaaaacgc
     7621 acactatatt gtcgctgccg agcgcgtccg ccgcgagggc ggccacgaga gccgaatcta
     7681 tccctccgga gagcccgatc acggtccctc tgaacccgtt cttcctcacg tagtcggcaa
     7741 ggccgagctt taaggcctcg tatacctcct ccacgacgtc gagacgcgtg acgggacgcg
     7801 gggtgagcgg tttcttgacc gaggggcgaa gcccgccggg tcgcgcggcc acggtggtta
     7861 cctgtccggc cgcagagacc ggacgggcct tcctgaggag ttccttttcc ggaatatcga
     7921 caacgagaag gtcttcccgg aacgcgcccg ccgcggcgac gaccgtgccc gagcggtcca
     7981 tcacgaagct ctgtccatcg aacacgagtt catcctggcc gccgacaagg ttgccgtagg
     8041 caacccacac ccggtcctca cgggtcctgc tcctgacgac gtcctcgcgg acgtaaatct
     8101 tccccgcatg atagggagag gcgtttatgt tgaagatgag cccggcgccc gcctgggcca
     8161 tgacatgtat ggggccttct ctgaaccaca tgtcctcaca gatcacgacg ccgaagagga
     8221 ccttgccgta gcggaacacc ctcggacggc tgccggggag aaagtatctc ttttcatcga
     8281 agacgccgta gttgggaaga agttccttgc ggaagacgcc ccgtatcttt cccttgtaga
     8341 tgacggcggc cgcgttatgg atgcccgacc gggagcggtc cacaaaaccg acgatcgtcg
     8401 ccggcgagtc gatacgcgac gcaatttcgg tgatgaccct gatgttggca tcgacgaaac
     8461 cttttttcag gagcaggtcc tcggggggat agcccgttat cacgagttcc ggcagcgcga
     8521 ggatgtccac ctcctcagcc tttgccttct ccacccactc tacgaccctt tctccgtttc
     8581 ccgtcatgtc gcccaccgtt gaattcatct ggcataaacc
//
LOCUS       DAOQTR010000016         8561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9167_length_8561_cov_3.233482, whole genome shotgun sequence.
ACCESSION   DAOQTR010000016 DAOQTR010000000
VERSION     DAOQTR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9167_length_8561_cov_3.233482"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..298)
                     /locus_tag="PKN85_00805"
     CDS             complement(32..298)
                     /locus_tag="PKN85_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42972.1"
                     /translation="MRCRVPVLVVMTMALLFSMAEGVSFAQSKVSCTDAVCGPGYRAY
                     LQPNGSCMCILADCESIEGCPEPPRVPEDCLSIEGCNPPQTIEP"
     gene            complement(506..1318)
                     /locus_tag="PKN85_00810"
     CDS             complement(506..1318)
                     /locus_tag="PKN85_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT42973.1"
                     /translation="MIKIVDYHKTFNAGTLNEVYALRGVDLHIAEGEFLTVIGTNGSG
                     KSTLLNAIAGTFLPDRGTITIGGQDMARKQDFRRARFISRVFQNPFMGTAPDMTIAEN
                     IHLAFMRGKHRYPVPGLTRERLAVYREEVRMLEMQLEDRLDNTIGTLSGGQRQALTLL
                     MAVMNGPRVLLLDEHTAALDPKSAAQVVALTERFIARYHLTTIMITHSMQQALALGSR
                     TILMHKGQVIEEIPGKDKQYLTTSDLLDRFADLRKQEKLTADMIEEMRREYL"
     gene            complement(1322..4147)
                     /locus_tag="PKN85_00815"
     CDS             complement(1322..4147)
                     /locus_tag="PKN85_00815"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNT42974.1"
                     /translation="MELWVGALNLGFLYAFVAIGIYITFRIHDFPDITVDGTFTTGAA
                     VTSVLVVAGTHPLLALLASFLACAAAGSVTALINTRLNVNSLLAGILVMTGLYSINLH
                     IMGRSNIPLLHTPEFFALLNRTNPGMPQEIWTALWLTVIMALSWLAVSSFFKTDFGLS
                     MRASGNNPVMARAAGINVDRVKIFGVAIANGLVGISGSLVAQYQGFADIGMGIGTVVT
                     GLASVIIGESILKKHSVYAWIVSAIIGSVIFRFMIAGALYAGMDPIDLKLLTAIFVLI
                     TLVVTAKIGKRTGRVKKKHLVTAAAAAAVILAVLAGIRYGNLASVMSQQRVKIGVFQV
                     SENPSLTITRDSFLEEMKRLGYEDGKNATFLLENANGDLATANAIVDKFLHEKVDIFV
                     PISTSCTQVAVKKVKEHPVVFATVADPFVTGAGKTDTDHLPNVTGVYGPVPMDRTMEI
                     VTKILPGRIRIGVIWDPSQANSEVNVNNLKKALEAYPRVTFVGGNIAGSSEVYQAASS
                     LVRRGIDAFVLPTDNIVYSAFDSVVKAARRKKVPIIFNDVGHPEGGALVVFGYAYETS
                     GRQAAHLVNRIIKGEEPATIPFERYKKTELGVNLKVAKESGLRIPEEILSAATIIVDE
                     RGGIHRRDGASSVEKMPGRGRKRLALFQFTDNVILDVCAKGVVDGLKKSGALEKEGIT
                     VDHKMAHGEFPTAQSIAQEMVSKNYDYIVTVSTISLQVTANHNRKIPHVFAAVTDPIK
                     AGVAKSFTDHPSNLTGLATPQPVEPTVRLMRRMFPRAKIIGMIWNPAEVNSEICTVMT
                     REAAKKYGFKVVEVQITGTQEIDQALRSILASEPDIFFTSGDTTVSAVIPSVAQKLRQ
                     KKIPYVTNTPADIDSGVFMCLGADYYDVGLKAAELAVRVIGGEKPAAIPIVSYVPEQL
                     TLNLSLAKEYGIKIPDDLIKKAARVVK"
     gene            complement(4151..5365)
                     /locus_tag="PKN85_00820"
     CDS             complement(4151..5365)
                     /locus_tag="PKN85_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNT42975.1"
                     /translation="MNDPLSPEQAVDKLLLAGRIDSITCREIEALFRNAIMAGKRLIV
                     ADMTAVSYVSSAGLRVFLSVQKELKKAGGEVCFLACPSTVHSVFEISGFTKIFRFLSS
                     ENEITRLLTSSAGEVEIKEIPYDRAALRVLTRDVPTGSLTLIGSEEKLPSASYGEGDV
                     VSVRAADMRFAAGFAALGHSFEDYKGYFGETIVLDGNIFVYPALRRSAVDFIVHTEGE
                     MGTVYHFLHGLSFTGDFAHIVSFEPGDNLFTLDALVESAHTVSSSPVIGITAIFESKG
                     LFGMRLKKIPLEENRRPGEEDIFSGKSFPEWVDYSIEAEDIYNLVVIAGISARQRDRV
                     TGDADRAIPREGRAHVHAIVFDKKPFNRTIDNFTNELRRIVTGMEPQRVIHLMGRSNI
                     GPGLMGIVELGG"
     gene            complement(5593..5772)
                     /locus_tag="PKN85_00825"
     CDS             complement(5593..5772)
                     /locus_tag="PKN85_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42976.1"
                     /translation="MGDKNSLVLYIIAFAVMVGGFVMIFLTRSTTVLAAGAVLIFIGL
                     FVFLFGREKAKKAKA"
     gene            6051..6248
                     /locus_tag="PKN85_00830"
     CDS             6051..6248
                     /locus_tag="PKN85_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42977.1"
                     /translation="MDLKSARDYLTKGAGFPPRRNVASGSGVPVAGCLFRDMMKDRGL
                     DGCRNRTCDCPFAGKKRREPH"
     gene            6535..6831
                     /locus_tag="PKN85_00835"
     CDS             6535..6831
                     /locus_tag="PKN85_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42978.1"
                     /translation="MDPNAPTVSRRTLRFFDGTQVAVNNLHEIMAELHSVGKEPNRKT
                     VDEIIAGLEAMGNYIPPSEATRREYRNVLLEEYKEFVETHDKEKRDATHPSEET"
     gene            complement(6866..7774)
                     /locus_tag="PKN85_00840"
     CDS             complement(6866..7774)
                     /locus_tag="PKN85_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT42979.1"
                     /translation="MGLTGEKRLAITLLVTLLIMTGEMIGGFLSNSLALLSDAGHMVT
                     DALAIALGLVAVHISRRPADKKATFGYQRVGLVAALLNGLSLLVIAVLIFHESYRRFL
                     APPVVDIPVMLSIAALGLAGNLVMAFILGHRHKDITLRSVWLHVLGDTLSSAGVIVSG
                     MVIHFTGWTYADPLASALIGCVIIWGGVRLVRDTMAIFLNLTPKGFNVESLVARIAGL
                     PDVMDVHDVHLWPVAHNNVAFSAHILVDDKTLGEVETTRKNIESILREAGVHHSTLQM
                     ECSCVECGGSLYCRSEPCDRDDTHGH"
     gene            complement(7818..8435)
                     /locus_tag="PKN85_00845"
     CDS             complement(7818..8435)
                     /locus_tag="PKN85_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT42980.1"
                     /translation="MKMTLYRRMVENITRMLVLSCILLGLVYSIPVICRSAETFTPSF
                     GKGTVKVRLYSDYFCPPCRAMEPGIEPVITDLVRDGTITLTFVDTPFYRYSSLYARCF
                     LYAINEKKDLEHALRARRALIEAAKNGLDSADKLEIFLKKEGITLKSFDPGPTFKAFS
                     RHLKEDAIDATPSCVIETEGGTKKYGGPGNIIDALKKLKKKQPET"
BASE COUNT         1785 a         2373 c         2575 g         1828 t
ORIGIN      
        1 atttcaggac ctgccgggag gggtccgcgc cctagggctc gatcgtctgc ggcgggttgc
       61 agccctcgat gctcagacaa tcttcgggga ctctgggagg ctccgggcag ccctcgatgc
      121 tttcgcaatc agccagaata cacatgcacg acccgttcgg ttgcagatat gccctgtaac
      181 cggggccgca cacggcatcg gtgcaggaca cctttgactg ggcgaacgag acaccctctg
      241 ccatcgagaa caagagggcc atcgtcatca cgacaagaac aggaacccga caacgcatac
      301 ccacacctcc tcagcgcggc cttactgcaa ttgatattat cctaatgcct gcacggcaaa
      361 gagtaaagca gaaaaaccgt tcacgccatc ccaatggtcc acggtccccc ctcaagctac
      421 tcaaacgact cacgccgtct ttctttctgt cttttgcctt tactctcaac aggctgtcaa
      481 gccattcaag ctgttcaagc cgcctctaca aatattccct cctcatctcc tctatcatat
      541 cggcggtgag cttctcctgc tttctcaggt cagcgaagcg gtcgaggagg tctgaggtgg
      601 tgaggtattg cttgtccttg cccgggatct cctcgatgac ctgacccttg tgcatgagga
      661 tggtccgcga gcccagcgcg agggcctgct gcatgctgtg ggtgatcatg atggtcgtaa
      721 ggtggtagcg ggcgatgaac cgctccgtga gggccacgac ctgggcggcc gacttcgggt
      781 cgagggccgc cgtgtgttcg tcgaggagca ggaccctggg tccgttcatc acggccatga
      841 ggagggtgag ggcctgcctc tggccgcccg aaagagtacc tatcgtgttg tcgagtctgt
      901 cctcgagctg catctcgagc atccgcacct cttcgcggta gacggccaga cgttcgcggg
      961 tgaggccggg gacgggatag cggtgtttgc ccctcatgaa cgcgaggtgg atgttctccg
     1021 ctatggtcat gtccggggcg gttcccatga aggggttctg aaaaacgcgg gagatgaagc
     1081 gggcgcgccg aaaatcctgt ttcctcgcca tgtcctgacc acctatggtt atcgtgccgc
     1141 ggtcggggag gaaggtgccg gcgatggcat tgaggagggt ggactttccg gagccgttcg
     1201 ttccgatgac ggtgaggaac tctccctcag cgatgtgcag atcaacgccc cggagcgcgt
     1261 agacctcatt gagggtgccg gcattgaagg tcttgtgata atcgacgatc tttatcatgg
     1321 gctatttcac cacccgcgcg gccttcttta tcaggtcatc gggtattttg atgccgtatt
     1381 ccttcgccag ggagaggttg agcgtgagct gttccggcac gtaggagacg atgggtatgg
     1441 cggcgggctt ctctcctccg atgaccctga cggcgagttc cgcggccttg agccccacgt
     1501 cgtagtaatc cgcaccgaga cacatgaaga cccctgaatc gatgtccgcc ggcgtgttcg
     1561 tcacgtaggg gatcttcttc tgccggagct tctgggcaac ggaggggatg acggcggaaa
     1621 cggtcgtatc tcccgaggtg aagaagatgt ccggttcgct cgcgaggata gagcgcagcg
     1681 cctggtctat ttcctgggtg ccggtgatct ggacctccac taccttgaac ccgtatttct
     1741 ttgccgcctc gcgcgtcatc accgtgcata tctcggaatt cacctcggcg gggttccaga
     1801 tcatcccgat gatcttcgcc ctcgggaaca tcctgcgcat gagccgcacc gtcggttcca
     1861 ccggctgagg ggtggcaagt cccgtcaggt tcgatgggtg gtccgtgaag ctctttgcga
     1921 ccccggcctt tatggggtct gtgacagccg cgaagacatg gggtatcttc ctgttgtggt
     1981 ttgccgtcac ctggagcgat atcgtcgaca ccgtcacgat gtaatcgtag ttcttgctca
     2041 ccatctcctg cgctatggac tgtgccgtgg gaaattcacc gtgggccatc ttgtggtcga
     2101 cggtgatccc ttccttctcc agggccccgc tttttttcaa accgtccacg acgcctttcg
     2161 cgcagacgtc gagaatgaca ttgtccgtga actggaaaag ggcgaggcgc ttcctgcctc
     2221 gtcccggcat cttttccacg gaagaggcgc cgtcccttcg atgtatgccg cctctctcat
     2281 cgacgatgat ggtggccgcg ctcaggatct cttcgggtat gcggagcccc gactccttcg
     2341 ctaccttgag gtttacgcct agctctgtct tcttgtaccg ctcgaagggg atcgtggcgg
     2401 gttcttctcc cttgatgata cgattcacga gatgcgcggc ctgcctgccg cttgtttcgt
     2461 aggcatatcc gaagaccacc agggcaccgc cctcgggatg gccgacatca ttgaagatga
     2521 tggggacctt cttgcgccgg gcagccttga ccacggaatc gaaggccgaa tagacgatgt
     2581 tgtccgtcgg caggacgaag gcgtcgatgc ctcgccggac cagcgacgag gctgcctgat
     2641 acacctccga agagcctgcg atgttgccgc ccacgaaggt gacgcgggga taggcttcaa
     2701 gcgctttctt gagattgttg acgttgacct cggaatttgc ctgcgagggg tcccagatga
     2761 ccccgatcct gatgcgcccc ggcagtatct tcgtaacgat ctccatcgtc ctgtccatgg
     2821 gcaccgggcc atagaccccc gtcacattgg ggagatggtc ggtatcggtc tttcccgctc
     2881 cggtgacaaa ggggtctgcc acggtggcga agaccacggg gtgttctttg accttcttga
     2941 ccgcgacctg cgtgcatgaa gtggagatgg gaacaaatat gtccaccttc tcatgaagga
     3001 atttgtcgac gatggcgttc gccgtcgcga ggtccccgtt cgcgttctcc agcaggaagg
     3061 tcgcgttctt tccatcttca taacccagcc gtttcatttc ctccaggaaa gagtcccggg
     3121 taatagtgag gctcgggttt tcactgacct ggaagacccc tattttgacc ctctgctgcg
     3181 acatgacgga ggcgaggttc ccataccgta ttcccgcgag gacggcgagg ataacggctg
     3241 ccgccgcagc cgcggtgacg agatgtttct tcttcaccct tcccgttctt ttgccgatct
     3301 tcgcggttac aacaagggtt atgagaacga agatcgccgt cagaagcttg aggtctatgg
     3361 gatccatacc cgcgtagagc gcgccggcga tcatgaaccg gaagatcacg gagccgatga
     3421 tggcgcttac tatccaggcg tagacggagt gcttcttgag gatggattcg ccgatgatga
     3481 cggaggcaag gccggtgaca accgtcccga tccccatccc gatatcggcg aaaccctgat
     3541 actgggcgac caggcttccc gagatgccga cgagcccgtt ggcgatggca acgccgaaga
     3601 tcttgacgcg gtccacgttg atccccgcgg cgcgcgccat gaccgggttg ttgccgctcg
     3661 ccctcatgga aaggccgaaa tcggtcttga aaaaactgga gacggcgagc caggataagg
     3721 ccatgatgac ggtgagccac agcgccgtcc atatctcctg gggcatgccg ggattcgtcc
     3781 tgttgagcag cgcgaagaac tccggcgtgt gaagcaatgg tatgttggag cggcccatga
     3841 tatgcaggtt gatggaataa agccccgtca tgacgaggat gcccgcaagc aggctgttga
     3901 cattgagacg cgtgttgatg agcgccgtga cgctccccgc cgcggcgcag gcaaggaaag
     3961 atgccagaag ggccagaaga ggatgggtgc ctgccactac gagaacggag gtaacggccg
     4021 cccctgtcgt gaacgtcccg tcaacggtga tgtcgggaaa gtcatgaatg cggaaggtaa
     4081 tgtaaatgcc gatggccacg aaggcgtaga gaaagccgag gttgagggcg cctacccaga
     4141 gctccatcga ctaaccgccg agctccacga tacccatgag gccgggaccg atgttcgacc
     4201 tgcccatgag gtggatgacc ctctgcggtt ccatgccggt gacgatcctc ctgagctcgt
     4261 tggtaaagtt gtctatcgtc cggttgaagg gtttcttgtc gaaaacgatg gcgtgcacgt
     4321 gggcgcggcc ctcccgcggg atggccctgt cggcatctcc ggtcacgcgg tccctctgac
     4381 gggccgagat gcccgcgatg acgacgaggt tatagatatc ctcggcctcg atggaatagt
     4441 cgacccactc gggaaagctc tttcccgaaa agatatcttc ttcgccgggg cggcggttct
     4501 cctcgagggg gattttcttg aggcgcatgc cgaagaggcc cttgctctcg aagatcgccg
     4561 tgatgcctat cacgggtgag gacgatacgg tgtgagcgct ttctacaaga gcgtcgagcg
     4621 tgaagaggtt atcgccgggt tcgaaggaga cgatgtgggc gaagtctccc gtgaaggaca
     4681 ggccgtggag aaagtgatag accgtgccca tctctccctc ggtgtgcacg atgaaatcaa
     4741 cggcggacct tctcaaagcg gggtagacaa agatattgcc gtcgaggacg atggtctctc
     4801 cgaaatagcc cttgtagtcc tcgaaactgt ggcccagagc cgcgaatccg gccgcaaacc
     4861 gcatgtctgc cgccctgacg gacacaacat ccccttcacc gtaggaggct gacggaagct
     4921 tttcttcgct gcctatgagt gtgagtgacc cggtggggac atcccgggtg aggacacgca
     4981 gcgccgcgcg gtcgtacggg atctccttta tctcgacctc tcctgcagag gatgtgagga
     5041 gtcgtgttat ctcgttttcg gaggaaagga aacgaaagat cttcgtgaat ccgcttatct
     5101 caaatactga atgaacggtg gacgggcatg ccaggaagca gacctctccc cccgcctttt
     5161 tcagttcctt ctgaacggac aggaaaacac gcagaccagc gctgctcaca tagctgaccg
     5221 ccgtcatgtc ggcgacgatg agcctcttac cggccatgat ggcgttcctg aagagggcct
     5281 cgatctcccg gcaggttatg ctgtcgatcc tgcctgcgag cagaagtttg tcgacggcct
     5341 gttctgggga caaaggatcg ttcatctgga gggtctcacc agtaagctat cattggaaat
     5401 cccactttgc aaatcttttg tgtctaaccg ggcggaacgg ggaaggagcc ggcatgcacg
     5461 gtcctccgag aggagcaaaa ctcaattcaa aggggacgta tcggatccgt taaaggcgac
     5521 aacgatcggc ggtatgaagg acgcaaaccg ggatttcaag agtgaggtgt tacagggtct
     5581 aacacgagcc ggtcaggctt tggccttttt ggccttttcc cttccaaaga ggaaaacaaa
     5641 gagcccgata aagatgagca cggcgccggc ggcgagcact gttgtgctcc gtgtcaggaa
     5701 gatcatgacg aaaccaccga ccatgaccgc gaaagcaatg atatacagga ccaaggaatt
     5761 cttgtcgccc atgccaaccc ccgtcgctta ggatatttta ctatagcata cattccgttt
     5821 ttatcaaaaa aaatactgca tcatttcggg ccattcaggt cttttcggca aaaaagtatt
     5881 aagaggtcat cgctggcgcc gataatacgg ttgtattggc acgttgggat ggaagggtct
     5941 gttttgagta ccagccggaa atgccgccca gggtgagccc ggatggcact atgacccggt
     6001 gcgcgaacag cgtatgacga gagcaacagg cgaaaggcag gaaacggaca atggatctta
     6061 agagtgccag agactatcta acaaagggag ccggctttcc gccgcggcgc aacgtggcgt
     6121 ccgggtcagg tgttcccgtg gcagggtgcc tcttccgcga catgatgaag gaccgcgggc
     6181 tggatggttg ccggaacagg acctgcgatt gcccctttgc cgggaagaaa cgccgggagc
     6241 cgcactgact tccgacggga gccggaaaga aaaaaaacag cttgacaatc atttttcggt
     6301 ggtcctgata tatatgtgga gtaccgcata gtatggtgcg aaaaatggtt gaggacgaca
     6361 tatccgtaac ttcctccgcg atggcagtgt gcctggcggc atgctcctcc atatccgcac
     6421 tcgaggtccg ggtcacaccg catcacgcac tgaagaccgg tccgtcagac cggccgggaa
     6481 taccagggag agcgctgcat gtctattttt caggttatag ggtgagttac ctgagtggat
     6541 ccgaacgcgc cgacagttag ccggcgtaca ctcaggttct ttgacggtac acaggtcgcg
     6601 gtcaataatc tccacgagat aatggcggag ttgcactctg tcggcaagga gccgaacaga
     6661 aaaaccgtgg atgagatcat tgccggactg gaagccatgg gcaattacat acccccttcc
     6721 gaggccactc gaagggaata cagaaatgtc ctcctggagg aatacaagga gtttgtcgag
     6781 acgcacgaca aggagaagag ggatgccacc catccttcgg aggaaacctg aacggtcaaa
     6841 ggagcccctc ggtgagggag caccgtcagt ggccgtgcgt atcgtcacgg tcgcaaggtt
     6901 ctgatcggca atagaggctg cctccgcact ccacgcaact gcattccatt tgcagggtgc
     6961 tgtggtgtac gccggcttcc cgcaggatgc tctcgatgtt cttcctcgtt gtttcgacct
     7021 ccccgagggt cttgtcgtcg acaaggatgt gggcggaaaa ggcgacgttg ttgtgggcca
     7081 cgggccagag atgaacgtcg tggacgtcca tgacgtcggg taacccggct atccgggcaa
     7141 ccagggactc cacattgaac cccttcggcg tgaggttgag gaatatggcc atcgtgtccc
     7201 gcaccagcct cacgccgccc cagatgatga cgcagccgat gagggcgctg gccagggggt
     7261 cagcataggt ccacccggtg aaatggatca ccatgccgga gacgatgaca cccgcggaag
     7321 aaagggtatc gccgagaaca tggagccaca cgcttctgag agtgatatcc ttgtgccgat
     7381 gtcccagtat gaatgccatg acaaggtttc cggcgagacc cagggccgcg atggacagca
     7441 tcacggggat atccacgacg ggaggcgcga gaaagcgccg gtaggattcg tgaaagatga
     7501 ggaccgcgat aacgagcagg ctcaggccgt tcaagagcgc ggcgacaaga cccacgcgtt
     7561 gatagccgaa cgtcgctttc ttgtcggccg gcctcctgct tatgtggaca gccacgagcc
     7621 ccagggctat cgcgagggca tccgtgacca tgtggccggc gtcgctgagg agcgcgaggc
     7681 tgttgctcag aaatccgccg atcatctcgc ccgtcattat gagaagggtt acaaggaggg
     7741 ttatcgcaag ccgcttttcg cctgtcaggc ccatgacata gtataggaca tctcccggag
     7801 ttccgggagt tcttcctcta cgtttccggc tgtttcttct tcaacttctt caaagcgtcg
     7861 attatgttgc ctggcccgcc atacttcttt gttccgccct ccgtttcgat cacgcatgac
     7921 ggggtggcat caatggcgtc ttccttcaga tggcgcgaga acgccttgaa ggtgggcccg
     7981 ggatcgaagg acttcagggt tatcccttcc ttcttaagga agatttccag tttgtccgcg
     8041 ctgtcgagac cgttcttcgc cgcttcgatc agggcccgtc gcgcgcgcag cgcgtgttcg
     8101 aggtctttct tctcgttgat ggcgtaaagg aaacaccgcg cgtagagcga agaatacctg
     8161 tagaacggcg tgtccacaaa ggtgagagtt atggtgccgt ctcgtaccag atcggttatc
     8221 acgggttcta tgccaggttc catggccctg caggggggac agaaataatc ggagtagagc
     8281 ctgaccttca ccgtcccctt gccgaaagaa ggggtgaatg tctccgctga ccggcagatg
     8341 acagggatac tatatacgag gccgagaaga atgcaagaca ggacaagcat cctcgtgata
     8401 ttttcgacca ttctcctgta cagcgtcatt ttcatctaaa ctgtctcctc atggtggtca
     8461 tttcgctgaa cgccttgaag ggggtatgtc ctgtcccccg gggaaagaga attgaatgtg
     8521 tacgagtagg aaggtgcgag agatcccttg aaaaagaggg a
//
LOCUS       DAOQTR010000017         8546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9189_length_8546_cov_4.377223, whole genome shotgun sequence.
ACCESSION   DAOQTR010000017 DAOQTR010000000
VERSION     DAOQTR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9189_length_8546_cov_4.377223"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /gene="lptA"
                     /locus_tag="PKN85_00850"
     CDS             <1..538
                     /gene="lptA"
                     /locus_tag="PKN85_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNT42981.1"
                     /translation="KVDAVQTAKHAETPGATRPETKEEGKTAPEDKPKARGVDKALGF
                     LDQKKPIDIVSDTMEGFDKEKYVFFKGSVVAKQDDLYIYSDTMEAFMSADTNEIEKAN
                     AKGNVRIIKQNRTATCKEAFFDNIKGEIVLKGNAVVTSDKDKVEGDIVTYYVNEDRAV
                     VTAEKSKKAKVTIYPQQK"
     gene            complement(817..1248)
                     /locus_tag="PKN85_00855"
     CDS             complement(817..1248)
                     /locus_tag="PKN85_00855"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNT42982.1"
                     /translation="MEDGKRNAYFDRVEKEPFARLLDIRLRDVSEGYALCEMCYTGDM
                     DNIYGNAHGGAIFGLIDEAFEISSNSHDRVAVALNMNITYMKPPEKGALLVAESKETH
                     KTNRTASYYITVKDRDDTIAVCQALVYRKSDPIPFLPEGTA"
     gene            complement(1252..1575)
                     /gene="trxA"
                     /locus_tag="PKN85_00860"
     CDS             complement(1252..1575)
                     /gene="trxA"
                     /locus_tag="PKN85_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNT42983.1"
                     /translation="MSNARAVTDGEFRTEVLESSIPVLVDFWATWCGPCQVMGPVIDA
                     LAGEYEGKVKILKLNVDENPQTPAQYGVRGIPTLIIFKNGAESGRIVGAQPKGNVDDA
                     LKKVL"
     gene            complement(1669..1881)
                     /locus_tag="PKN85_00865"
     CDS             complement(1669..1881)
                     /locus_tag="PKN85_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42984.1"
                     /translation="MRAFVPYLWGYKSAKSVIRVTLMDRYVSGYWEERGYTDEAYLEA
                     GLVRDMNDGGRIRRISEEEWKECLDD"
     gene            complement(1878..2273)
                     /locus_tag="PKN85_00870"
     CDS             complement(1878..2273)
                     /locus_tag="PKN85_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT42985.1"
                     /translation="MSRLKELRTPVFWAEGHPGVLDREGWEIEVTGLCDAPRTFTWAD
                     LRAMPKTVVDARLTSVTRFTVRGNWGGVRVLDIMNAVKAHPSVAFVRFWSIKRIYDTS
                     IPVETAVRSKTLLAYEFDGEYLEEDYGGP"
     gene            2424..3596
                     /locus_tag="PKN85_00875"
     CDS             2424..3596
                     /locus_tag="PKN85_00875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNT42986.1"
                     /translation="MVKLTVRKERSGPVRGFHPWVFSKALRSIPEGLVPGEPVELCDE
                     SGSFLASGYFNSYGQISVRLWGHEKDEPVGTDFFVRRLERAADIRRRYVERPGTNAYR
                     LINGESDLIPGLIVDRYGDHLVVQFHTRGMERWKTEVVESLVAVFGPRGIWERSDVSV
                     RNIENLGAVRGLLHGSVPDAIEIAENGLRFLVDVKEGQKTGFFLDQRDKRSAFTKYAK
                     DREVLNCFSYTGGFTVYAVAGGARRVVSVDSSRRALDIAKENIILNGFDTSRCEFVCD
                     DAKRYLREPGRHFEATVLDPPAFIKDRRKKQEGLAGYRAINEMALRNLSPGGVLVTCS
                     CSAHLSLSDFRFLLSEAGAKTGRPLTLLETHTHGIDHPQLVPFTEGEYLKCFILRG"
     gene            complement(3706..4470)
                     /gene="hisF"
                     /locus_tag="PKN85_00880"
     CDS             complement(3706..4470)
                     /gene="hisF"
                     /locus_tag="PKN85_00880"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNT42987.1"
                     /translation="MKTVKIMPCLDMKNGRVVKGVHFVDLKDAGDPVENAAYYQKEGA
                     DELAMLDIAATVENRKTRLEWVKEVSSVITIPLTVGGGINTTDDIELVLKAGADKVSM
                     NSAAVKDPTLVKEAADRFGGGRIVIAVDARRNGAMPSGFELVVAGGTTPVGKDAVEWA
                     KRCQELGAGTILPTSMDGDGTLKGYDLEFTKAISDAVTLPVIASGGAGTLEHFYEGVV
                     RGGADILLAASVFHFRTFTVRQVKEYLKGKGVAVNL"
     gene            4730..6376
                     /locus_tag="PKN85_00885"
     CDS             4730..6376
                     /locus_tag="PKN85_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT42988.1"
                     /translation="MKIEKLRNLGIFYKIMGISAVAIVVIILWNILYLLPQMERIMLK
                     EKQNAVKDVVDVAFSMLNDYDKRVKAHEIEMGMAQMAAIKSIQQLRYRGENYFWINDT
                     QPKMIVNPVMIDLEGQDVSGFEDPKTKKKIFVEMTAIAKDRKAGFYEYNWKRSGATSF
                     STKQSYIKYFEPWGWVIGSGMYLDDVNREIAKFRWIIIGTTLVLALLVLALSYLIANN
                     IRKNLSKAVDASRSLSEGDLSIDISANGSDETGQLLQSMKNMIGSFNTMIGSIKSSVD
                     SLDTSVETIKTLADRTSEGTRTQADQANQISVSAEEMSQTIVDVANNASGVKDASTNA
                     MEAAITGKKVTQEAVAEVNKVQHSSTELANTIQKLNQRVSEVAKIITIIDDIADQTNL
                     LALNAAIEAARAGEHGRGFAVVADEVKKLAEKTMNATKEITNHLTTVQRESEETTRSM
                     EITSAEVERVTEYMQEVEQSLGNIVSSSEQVRDQIMQIATAVEQQSASSEEIARNITS
                     TSGIAKEIEAVSSQVMSEVNKLVEITQVLGSSAAQFKTKA"
     gene            complement(6469..7002)
                     /locus_tag="PKN85_00890"
     CDS             complement(6469..7002)
                     /locus_tag="PKN85_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT42989.1"
                     /translation="MAEIKSAIELAMEKTKGLVMDDREKRSLALKELAAGMMTIYRRY
                     REGLSGDDETKAQLDALECDSAGKRKIALDILGDEFEAGDDVAQMAPLFSFIGFVVDE
                     KARRELLALQKECLGELEKIRGSIASRITQDLAASGIKGDSVEPNIEAWPVWKKASSD
                     VRRAFRRQIDKWKEGLL"
     gene            complement(7005..7400)
                     /locus_tag="PKN85_00895"
     CDS             complement(7005..7400)
                     /locus_tag="PKN85_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT42990.1"
                     /translation="MDRVIIEHLPENKDLAGAKRWVDEKGEFVQISWREDIGHLAFFE
                     LRKGQFRGGHYHERKEEVFYVVSGTIRAVFADLGSGGRETRILEKGMKVRVGTRVGHR
                     FEGIEDSLVIEYSPQYYDKTDALRADLGE"
     gene            complement(7403..7945)
                     /locus_tag="PKN85_00900"
     CDS             complement(7403..7945)
                     /locus_tag="PKN85_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNT42991.1"
                     /translation="MLYEYDGRRPTVGKDSYVSDIAHVIGDVVIGDNCYIGHGAILRG
                     DYGAIVVGSGTAVEEGVVVHAPPDRYCRIGKRVTIGHGAVIHAASIGDSAVIGMGAVL
                     SIESEIGEGTIVAEGTVVRMRQKVTARVVVGGNPMRVIRQIAPKDMEFWDKGKQLYID
                     LAKKYLEKGMRPLQGPPGGA"
     gene            complement(7983..>8546)
                     /locus_tag="PKN85_00905"
     CDS             complement(7983..>8546)
                     /locus_tag="PKN85_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNT42992.1"
                     /translation="TGKNLTEDRKSEMLVANVKKMPLDKAIKIGNGKQTVIEFTDPDC
                     PYCRRAASYFESRKDLTRYVFFFPLPSHKDAENKIKYVFCSSDRAKAYEEAMKGKLDT
                     QKYKVCKKQEAADLLNAHKQIGVQIGVSGTPMFIINGKDLVMGANFPAVEAALRTDDQ
                     KQGSSEQKTGAPEKKPAEPEKKATEGK"
BASE COUNT         1823 a         2564 c         2331 g         1828 t
ORIGIN      
        1 gaaggtggac gctgtgcaga cggcgaaaca cgccgaaacc cccggcgcca cacgtcccga
       61 aaccaaagaa gagggcaaga cggccccgga ggacaagcca aaggcaaggg gcgtggacaa
      121 ggcgctcggc tttcttgacc agaagaaacc catagatatc gtctccgata caatggaagg
      181 cttcgacaag gaaaaatatg tcttcttcaa gggcagcgtc gtggccaaac aggatgacct
      241 ctacatctat tccgatacga tggaggcctt catgagcgcc gacaccaacg agatagagaa
      301 ggcgaacgcg aagggcaacg tgaggatcat caagcagaac aggacggcaa cctgcaaaga
      361 ggccttcttc gacaacatca agggcgagat cgtcctcaag ggaaacgccg tcgtgacgtc
      421 ggacaaggac aaggtcgaag gcgatatcgt cacatactac gtaaacgaag accgcgccgt
      481 ggtcaccgcg gaaaaaagca agaaagcaaa ggtgaccata tacccccaac agaaataaag
      541 acggcttaaa tagcttgagc cgcttgaatg gcttgaaagt gaaaggcaag aacctttgtt
      601 cgccgacaac ggttcttgcc ttttgttcta acgctcaaca ggctgttgac aaagtgattt
      661 ctgaagacga ttcgtgttct ttcgtcattc cggcgaaggc cggaatccag gaaaaacgag
      721 gggttagaga aacctctgga ttccggcctt cgccggaatg acgtagaaaa tgggtttatc
      781 aacagcctgt caaacgactc aagccattca agcggctcaa gccgtccctt ccggcaggaa
      841 gggtatgggg tcggacttgc ggtagacgag ggcctggcac acggctatgg tgtcgtcgcg
      901 gtcctttacc gttatatagt aggaagccgt cctgttcgtc ttgtgtgttt ccttcgattc
      961 agcaacaagg agggcaccct tttcgggtgg tttcatatag gttatgttca tgttcagggc
     1021 aacggccacc ctgtcatgac tgttcgacga gatctcgaag gcctcgtcga tgagcccgaa
     1081 gatcgctccg ccatgggcgt tgccgtagat gttgtccatg tcccccgtgt agcacatctc
     1141 gcacagggca tatccttcgc tgacatccct gagccgtatg tcgagaagac gcgcgaaggg
     1201 ctctttttca acccgatcga aataggcgtt cctttttccg tcctccatcc ctcacagcac
     1261 cttcttcaag gcatcatcga cgttgccctt gggctgcgcc ccgacgatgc gtcccgattc
     1321 ggcgccattc ttaaagatga tcaacgtggg gatgccccgg accccgtatt gcgccggcgt
     1381 ctgggggttc tcgtcgacat tgagcttgag gatcttcacc ttgccctcat attcaccggc
     1441 aagggcatcg atcacgggac ccatcacctg gcatggcccg caccaggtcg cccagaaatc
     1501 cacgagaaca gggatgctcg attcgagaac ctcggttctg aactctccgt ccgttaccgc
     1561 tcttgcgttg ctcatgcctt cctccttgtt cgttgtccgc cacaggaccc ccgggcaggc
     1621 aatgttttta taatagttaa gcccttcgcc ggtcgttgtc aaccatgttc agtcatcgag
     1681 gcattccttc cattcctcct cggagatgcg ccgtatcctt ccgccatcgt tcatgtcgcg
     1741 cacaagcccc gcttctaggt atgcctcatc ggtgtacccc ctttcctccc agtagcctga
     1801 cacgtagcgg tccataaggg tgacgcgaat gacgctcttc gccgacttgt agccccagag
     1861 gtagggcacg aaggccctca cgggcccccg taatcctcct cgaggtattc cccgtcgaat
     1921 tcgtaggcca ggagggtctt gctccttacg gccgtttcca ccggtatgga cgtgtcgtag
     1981 atcctcttta tcgaccagaa cctcacaaaa gcaacggaag gatgcgcctt cacggcgttc
     2041 atgatgtcga ggaccctgac cccgccccag ttgccccgca cggtgaagcg cgtcacgctc
     2101 gtcaggcggg cgtcgacgac cgttttcggc atcgccctga gatcggccca cgtgaatgtc
     2161 cgcggcgcgt cacagagtcc tgtcacctct atctcccatc cttccctgtc aaggaccccg
     2221 gggtgcccct cggcccaaaa aacgggtgtt ctcaactcct tcaaccggct catcgcgacc
     2281 tcccgcccta aacaataatc atagccgaat ttcaggggtc cgcaaataaa aaagaccacc
     2341 cgggagcaaa gccctcatat gccctatggc tcctgggcct cttcctgttc catttttccc
     2401 gcgattgttc tatacttgcg accatggtca agctcacggt aagaaaggag cgcagcggac
     2461 ccgtcagagg gttccatccc tgggtgtttt cgaaagcgct caggtccata ccggagggtc
     2521 tcgtgccggg tgaacccgtc gagctttgtg atgagtccgg ttccttcctg gcatcgggct
     2581 acttcaactc ctacggacag atatcggtga ggctctgggg acatgagaag gacgagcccg
     2641 tcggaacgga tttcttcgtc cggaggttgg agagggcggc tgatatccgc cgccgctacg
     2701 tggaacgccc gggcacgaac gcctatcgcc tgatcaacgg tgaaagcgat ctcattccgg
     2761 gacttatcgt ggacaggtac ggtgatcacc tcgtggtcca gttccacacc cggggtatgg
     2821 aacggtggaa gaccgaggtc gtggagtccc tcgtagcggt attcgggcct cggggtatct
     2881 gggaacgctc cgatgtgtcc gtgaggaaca tcgagaacct cggcgccgtt cgggggcttc
     2941 tccacggcag cgtccccgac gcgatagaga tcgcagagaa cgggctcagg tttctcgtcg
     3001 atgtcaagga aggacagaag acggggttct ttctcgacca gcgagacaaa cgcagcgcct
     3061 tcaccaaata cgcgaaggac agggaggtcc tcaattgctt ctcctatacg ggagggttca
     3121 cggtctacgc cgtggccggg ggcgcccgcc gcgtcgtcag cgtcgactct tcccggcgcg
     3181 ccctcgacat cgcgaaggag aacatcatcc tcaatggatt cgacacgtca cggtgcgaat
     3241 tcgtctgtga cgacgcgaag aggtatctca gggagccggg ccggcacttc gaggcgaccg
     3301 tgctggaccc tcccgcgttc ataaaggacc gcaggaagaa acaggaaggc cttgccggct
     3361 accgggcgat caacgaaatg gccttgagaa acctctcgcc cgggggcgtc ctcgtgacat
     3421 gctcctgttc cgcccatctg tccctgagcg atttccgctt cctcctgtcg gaggcggggg
     3481 caaagacggg aagaccgctc accttactcg aaacccacac ccacggcata gaccatcccc
     3541 agctcgtacc gttcacggag ggggaatacc tgaaatgttt catcctgagg ggatgaaaca
     3601 tttcccgttt taagtgattg agttttaagt gttaagtcaa aggcaggagc agaaaggatt
     3661 tcatctttta cttaaaactt aaaacctaaa acttaaaacc gtcttttaca ggtttaccgc
     3721 cactcccttc cccttcaaat actccttcac ctggcggacc gtgaaggtgc ggaagtggaa
     3781 gaccgatgcg gccagcagaa tgtccgcgcc accacgcacg acaccttcgt agaagtgctc
     3841 cagggttccc gctccgcccg aggcgatcac gggcagggtc accgcgtcgg atatggcctt
     3901 cgtgaactcg aggtcgtatc ctttcagggt cccgtcgccg tccatgctcg tcggcaggat
     3961 ggtaccggca ccgagttcct ggcagcgctt cgcccattcc acggcgtcct tgccgacggg
     4021 ggtcgtaccg cccgccacga caagctcgaa acccgagggc attgccccgt tacgcctcgc
     4081 atcgacggcg atgacgatcc tcccgcctcc gaacctgtcg gcggcttcct tcaccaaggt
     4141 cgggtccttc acggcggcgc tgttcatgga caccttgtcg gccccggcct tgaggacgag
     4201 ctcgatgtca tccgtcgtgt tgatgccgcc tcccacagtg aggggaatgg tgatcaccga
     4261 ggagacctcc ttgacccatt ccagccgggt cttgcggttt tcgacggtgg ccgcaatgtc
     4321 gagcatcgcg agttcatccg ccccttcctt ctggtaataa gccgcattct cgacgggatc
     4381 tccggcatcc ttgaggtcaa caaaatggac acctttcaca acgcgtccat ttttcatatc
     4441 gagacatggc atgatcttca cggtcttcat tgtcaggctc ctcatctttt ggatttccgt
     4501 ccggcctccc cggccggcgg cggttcataa atacatattc gctttcttca tcccctgtca
     4561 aggagccttc ttccgcatct ccgggaaatg ccttgcgagc ccctgtaaat tcttgtaaag
     4621 ttatctgacg ggcacccgat aatatcgtta aaggcatatt tcaccacctt gcgtcgatca
     4681 gaaggcctct gccatacgca ttgaaaaatg aaccgggagg tgtgttcacg tgaagatcga
     4741 aaagctcagg aacctcggca ttttctacaa gatcatgggg atctcggccg ttgccatcgt
     4801 cgtcatcatc ctctggaaca tcctgtatct tctccctcaa atggagcgga tcatgctgaa
     4861 ggagaaacag aacgccgtca aggacgtcgt cgatgtcgcg ttcagcatgc tcaacgatta
     4921 tgacaagagg gtgaaggctc acgagatcga gatgggcatg gcccagatgg cggcgataaa
     4981 gtcgatccag cagcttcgtt atcgcggcga gaattatttc tggataaacg acacgcagcc
     5041 caagatgatc gtgaaccccg tcatgataga cctggaagga caggacgtgt cgggttttga
     5101 ggaccccaaa acgaaaaaga agatcttcgt cgagatgacc gccatagcga aggatagaaa
     5161 ggcgggcttc tatgaataca actggaagag gtcgggggcg acgtccttct ccacgaagca
     5221 atcctacata aaatacttcg aaccctgggg gtgggtgata ggttcgggca tgtatctcga
     5281 cgacgtgaac agggagatcg cgaaatttcg ctggatcatc atcggcacca ctctcgttct
     5341 agcccttctc gtgctcgcgt tgagctacct catcgccaac aacatacgaa agaacctcag
     5401 caaggccgtc gatgccagca gatccctgtc ggaaggcgat ctcagcatcg acatcagcgc
     5461 caacggttcg gacgaaacgg gacagcttct gcagtcgatg aagaacatga taggctcctt
     5521 caacacgatg atcggcagca tcaaatcatc cgtcgacagc ctcgatacgt cggtcgagac
     5581 catcaagaca ctcgccgaca ggacgtcgga gggaacacga acacaggccg atcaggccaa
     5641 ccagatatcg gtctcggccg aagagatgag ccagaccatc gttgatgtcg caaacaacgc
     5701 cagcggcgtc aaagacgcct cgacgaacgc gatggaggcc gcgataacgg ggaagaaggt
     5761 cactcaggag gccgtcgccg aggtcaacaa ggtccagcat tcctccaccg aactggcaaa
     5821 cacgatccag aaactcaacc agcgcgtcag cgaggttgcc aagatcataa ccataataga
     5881 cgacatagcc gaccagacga acctactggc gctcaacgca gccatagagg ctgcgcgcgc
     5941 aggagagcac ggacggggct ttgccgtcgt cgccgacgag gtgaagaagc ttgccgaaaa
     6001 gacgatgaac gcgacaaagg agatcacgaa ccatctgacc acggttcaaa gggaatcgga
     6061 ggaaaccacg aggtccatgg agatcacctc agccgaggtt gaaagggtga cggagtacat
     6121 gcaggaggtc gagcagtcgc tggggaatat cgtctcgtcc tccgaacagg ttcgcgacca
     6181 gatcatgcag atcgccaccg ccgtcgagca gcagtcggcg agttccgaag agatagcgag
     6241 gaacatcacc tccacctccg gcatcgccaa ggagatcgag gcggtgtcca gtcaggtgat
     6301 gtccgaggtc aacaaactcg tcgagataac gcaggtactg ggaagctccg cggcccagtt
     6361 caagacaaaa gcctgaaggc ggctttaagg gggttacagg gtaacggggt gagccgctgg
     6421 aagggatgcg ggcatacggg ctttcttccg tgtgcccctt cttttggctt acagaaggcc
     6481 ttccttccat ttgtctatct gccgcctgaa ggcgcggcgg acatccgagg acgccttctt
     6541 ccataccggc cacgcctcga tattgggctc cacgctgtct cccttgatgc ccgacgcggc
     6601 aaggtcttgg gttatccggg atgcgatcga gcctcgtatc ttttcgagtt ccccgaggca
     6661 ctccttttga agggccagca attccctgcg ggccttctca tcgacgacga aaccgatgaa
     6721 cgagaagagg ggggccattt gggcaacatc gtccccagcc tcgaactcgt cgccaaggat
     6781 gtcaagggcg atctttctct tcccggcact gtcgcattca agcgcgtcga gttgggcttt
     6841 tgtctcgtca tcgccggaga gaccctcacg ataacgccgg tagatcgtca tcatcccggc
     6901 cgcaagctcc ttgagggcaa gggacctttt ttccctgtcg tccatgacga gacccttcgt
     6961 cttctccatg gccaattcga tggcactctt tatctccgcc atgatcactc tcccaggtct
     7021 gccctcaggg cgtcggtctt gtcgtagtac tgagggctgt actcgatcac gagggaatcc
     7081 tcgatgcctt cgaagcgatg gcctacacgg gtcccgacac ggaccttcat ccccttttca
     7141 aggatgcggg tctctctccc gccgctcccg agatcggcga agacggccct gatcgtgccg
     7201 ctgacgacat agaagacctc ctctttgcgc tcgtggtaat gaccgccgcg aaactgtccc
     7261 ttgcggagtt cgaaaaaggc caggtggcct atgtcctccc tccaggatat ctgtacgaac
     7321 tcgcctttct catcaaccca gcgcttggcg ccggcgaggt ccttgttctc ggggaggtgt
     7381 tcgatgatca ccctgtccat tgtcatgccc cgcccggcgg tccctgcagc ggtctcatcc
     7441 ctttttccag gtacttcttc gccaggtcga tgtagagctg tttgccctta tcccagaact
     7501 ccatgtcctt cggggcgatc tgtctgatca cgcgcatggg attcccgcct acgacgacgc
     7561 gcgccgtcac cttctgccgc atcctgacaa cggtcccctc ggcaacgatg gtgccctcac
     7621 cgatctcgct ctctatactg aggaccgctc ccatgccgat aacggccgaa tcaccgatgc
     7681 tcgcggcgtg gatgacggct ccgtggccga tggtgaccct ctttccgata cggcagtacc
     7741 tgtcgggggg cgcgtggacg acgaccccct cttccaccgc cgtgccgctt cccacgacga
     7801 tggcgccata gtctcccctg agaatggctc cgtggcctat gtaacagttg tcacctatca
     7861 cgacatcgcc gatcacatgg gcgatgtcgc ttacataact gtccttgccg accgtcggcc
     7921 ttcgtccgtc gtattcgtaa agcatgcgtc gttccgcctt ccgttcaccc cggggaagag
     7981 gctcacttgc cctcggtcgc tttcttttcg ggttcggccg gtttcttttc cggcgcgccc
     8041 gttttctgct ccgacgagcc ctgtttctgg tcgtccgtcc tgagggcagc ctctaccgcg
     8101 ggaaaattgg cacccatcac aaggtctttg ccgtttatga tgaacatcgg ggtgccgctc
     8161 actccgatct ggacccctat ctgcttgtgg gcgttcaaca gatccgctgc ctcctgcttt
     8221 ttgcacacct tgtatttctg cgtatccagc ttgcccttca tcgcctcttc gtatgccttt
     8281 gccctgtcgg aggaacagaa gacatacttg atcttgttct cggcgtcctt gtgggaaggc
     8341 agggggaaga agaaaacata ccgcgtcagg tccttcctgg actcgaaata tgacgcggcc
     8401 cggcggcagt agggacaatc ggggtcggtg aactctatga ccgtctgctt cccgttgccg
     8461 atcttgatcg ccttgtcgag gggcatcttc tttacgttgg ccacgagcat ttccgacttc
     8521 ctgtcctccg tcaggttctt gcccgt
//
LOCUS       DAOQTR010000018         8531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9206_length_8531_cov_4.719915, whole genome shotgun sequence.
ACCESSION   DAOQTR010000018 DAOQTR010000000
VERSION     DAOQTR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9206_length_8531_cov_4.719915"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..388
                     /locus_tag="PKN85_00910"
     CDS             110..388
                     /locus_tag="PKN85_00910"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNT42993.1"
                     /translation="MAENPVKRYGFIVFLSTIFFHLILMDGGIYDLLKVKLKTRTAKL
                     AIMQMEDENAALSRELRRIREDDQYLEEMVRKKYGLVREGEKVYRVEQ"
     gene            385..1260
                     /locus_tag="PKN85_00915"
     CDS             385..1260
                     /locus_tag="PKN85_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT42994.1"
                     /translation="MKYEGTIYRPPSEADSLILQVTIGCSHNKCTFCGSFKEKKFRVR
                     SFEEIAEDVNEAKQYARYIRKVFLADGDALIIPQKRLLPIVQLVRDAFPKMERIGIYG
                     NTKSILKKSVEELKALKELGVGIIYLGVESGDQVVLDRVCKGTTLDKTAEAAKRVKDA
                     GILLSVTVLLGLGGVERSRAHAEETGKFLSRIQPDYAGALSVIVVPGTVLADQIKTGE
                     FVVPDPYMLLEELYTMIQNTNVEHTYFASNHASNYLPVKAWLPEEKEKTLRAIQHVLA
                     ERDPSMLRPEFMRAL"
     gene            1263..1703
                     /locus_tag="PKN85_00920"
     CDS             1263..1703
                     /locus_tag="PKN85_00920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT42995.1"
                     /translation="MPGQANDHARDDGGFIDVPVRVRYADTDRMGIVYYGNYPAFFEM
                     GRSEYMRQKGYPYRDLETLGYHFVVVGLEAKYYNSATYDDILIVKTRVTELKSRGITF
                     HYVITRDGTLVVEGSTKHICVNTDKKPVTIPQKLIEIFRDGTAG"
     gene            1744..2748
                     /locus_tag="PKN85_00925"
                     /pseudo
     CDS             1744..2748
                     /locus_tag="PKN85_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
     gene            2753..4255
                     /locus_tag="PKN85_00930"
     CDS             2753..4255
                     /locus_tag="PKN85_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT42996.1"
                     /translation="MRFLFVYPNINTQVGFNYGISYISGFLRSRGIETGLLNINDKLG
                     YPMDMDRIRHDVLAFKPDIIGFSVLTNQHKYALDIARDIKTYLDVPILFGGIHVTMDP
                     METLMRDEVDLICVGEGEEAVAELVEKGDPRGIANIGWKEKGRPVIEPLRPYKDIASL
                     PFKDYEIFDFQRLIDAKDGWVGLTASRGCPFRCTYCLNHKIIEVYKESGHRPRGYIRR
                     HTVDQMISEIEYLLGRYTGIKMFIFDDDIFTFDKAWLREFTERYRAVTDISFVCNAHA
                     RIFDEETAELLKGAGCRIVKFGLESGSDRIRRKVLSRYMTNKHIEDAFAVAHRHGLHT
                     SAFVMLGLPHETIGDIKQTIDLIAAIKPGRFRWSLFFPFVGTKAYNIALSSGQVDLTE
                     MERLDNFTDETCMRLGPDVDLYCDKVKSALCAFVNAHSGLEHTEGFRRLAEEIENMDE
                     KTWREKKASILDRVEELNQEMEKSGGLHYTVRYNPFMGVRSDWKDDSISA"
     gene            4224..5219
                     /gene="waaF"
                     /locus_tag="PKN85_00935"
     CDS             4224..5219
                     /gene="waaF"
                     /locus_tag="PKN85_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNT42997.1"
                     /translation="MTGKTIVYLPNWLGDMVMAVPFLHALRQHSQDEVWAMGKESAIH
                     LYNGLGLFDRFVPISGKGLGLFFETTNRLNRVGFTRAIALPHSFRSTLVFFNLRVRDV
                     IGYARNRRGFMLNVKVPEARELQSTVEHYLRIADTLGVPRTIEAPVLSVSADEEKKFD
                     EEFSDMTRPYGVMIVGAQYGPSKCWPAGYFSEFADEIIERYGMNVYMLPGRGEDVLAR
                     KIHEGIRAGERARIRSMNIRDMKVCLSRAAFVVSNDTGPRHISAALSVPTVVLAGPMD
                     ERYTAYPGGHTRVVSKDMACRPCNKKKCDGNHECMTGIKPRDIAEIVGDILEKRV"
     gene            5203..6249
                     /locus_tag="PKN85_00940"
     CDS             5203..6249
                     /locus_tag="PKN85_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT42998.1"
                     /translation="MKRESEDKLRKPGRKAKERDGAGLIRFLDRFRGKKICVVGDIIA
                     DVFILGKPYRLSREAPVVVVKYEESKIYPGSAGNTINNLAALGATVYPVGFVGSDSMG
                     GKLMDFFSTRKTINTAGIIRVSGDTVTKTRILAGDTHVSKQQVIRIDKDDGRRRTTKE
                     KALLMERIAGICPHVDAVIVSDYGHGAVSGKVIEYMKILANDKIVVGDSRYGLKKYSG
                     FTMITPNESEAFALSGLRENEPIEDAGGKVLSGMRLSALLVTRGNKGMTLFLKDGKVH
                     HIPISGKDDVTDVTGAGDTVCAAVALSLASGAGFLDASRIANYSAGIVVMKRGTATVT
                     VAELKRSIENGGKI"
     gene            6317..6799
                     /locus_tag="PKN85_00945"
     CDS             6317..6799
                     /locus_tag="PKN85_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNT42999.1"
                     /translation="MGKIVNGLDELKAIIEREKAAGKRVVFGNGCFDIVHVGHVRYLR
                     GAKELGDVLVVAVNDDASVTGLGKRKEVVTPAAERAEIISAIDCVDYVILFADPTVEN
                     LLRELKPHIHAKGTDYTEGNVPERDIVMSYGGRVAIVGDPKDHSTRDIIKTIKNLREK
                     "
     gene            6932..7723
                     /locus_tag="PKN85_00950"
     CDS             6932..7723
                     /locus_tag="PKN85_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT43000.1"
                     /translation="MERQQDARPPLSVYMITLNNSATIARALESVAGWADEIIVVDSH
                     SSDGTADIASKYTEQVLQFDTTSQPEKYQYAQDRCRNRWVLFIDADEWLTEDFRKETE
                     TILASGSASDGYIADRRNIYLGREIKHGGWYPDREIRLYRKDRGGWEGGIHAKVHVKG
                     EVGHMRNHYLHTPYADSAHQIRTIDRYSEAYARDLLAAGRRFHMANMLLRPIWRFFRD
                     YIIKMGFLDGVPGIIIMASTMYYVFMKHARLWEMERDDGGKKGVR"
     gene            7698..>8531
                     /locus_tag="PKN85_00955"
     CDS             7698..>8531
                     /locus_tag="PKN85_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT43001.1"
                     /translation="MEERKVFDNKDVLVTGGLGFIGSNLCIELVRLGARVTIIDNMLP
                     RQGGNLFNIRDIADRVTVNVSDVRNQLSMNHLVKGKDYIFHLAGQVNHVDSMKNPLQD
                     LEINCTGTLVLLEALRHNNRDARVIFAGTRGEYGSSVRLPVDEDHPTNPKGIYAVTNL
                     TAEKMVLVYDDVYKIRGVCLRITNTYGPRHQMAHDEYGVFNWFIRKALDDEEIPVFGD
                     GRILRDFLYVEDLVDSILKVAAAEEAYGKVFNVGTGVPVSFFELAEKIVAIAGTGRTR
                     FT"
BASE COUNT         2019 a         2462 c         2498 g         1552 t
ORIGIN      
        1 cttttcttct gggtataccc cagggtggta agtatgtcca tgcccaacta ctggcgcccg
       61 cctctcacgg gttgacattt tgcttccgtg cctttaatat aaagaaagca tggcagagaa
      121 tcccgtaaag agatacggat ttatagtctt tctctcaacc attttcttcc accttatcct
      181 catggacggt ggaatatacg accttttgaa ggtcaaactg aaaacgagga cagccaaact
      241 ggcgatcatg cagatggaag atgagaacgc ggcgctctcg cgtgagcttc gcaggatacg
      301 cgaggacgac cagtacctcg aagagatggt aaggaagaaa tatggtcttg tgcgagaagg
      361 ggaaaaggtg tacagggttg aacaatgaaa tacgaaggaa cgatctacag gccgccaagc
      421 gaggcggaca gtcttatcct ccaggtcacc atcggctgct cccataacaa atgcactttc
      481 tgcggctcct ttaaagagaa gaaattccgg gtacgctcct tcgaggagat cgccgaggac
      541 gtgaacgagg caaagcagta cgcgcgctat atcaggaagg tcttcctcgc cgacggcgac
      601 gcgttgatca tcccccagaa aaggctcttg cccatcgtgc agctcgtcag ggatgccttt
      661 ccgaagatgg aacgcatagg catctacggg aacacgaaat cgatcctgaa gaaatccgtg
      721 gaagagctga aggccctcaa ggaactcggc gtcggcatca tctacctcgg cgtggagtcg
      781 ggagaccagg tggtcctcga cagggtctgc aagggaacaa ccctcgacaa gaccgccgag
      841 gccgcgaaga gggtgaagga cgcgggcata ctcctttccg tgaccgtcct tttggggctc
      901 ggcggggtgg agcggagcag ggcacacgcg gaagagacgg gaaagttctt aagcaggatc
      961 cagcccgact acgcgggggc cttgagcgtc atcgtcgtgc ccggcacggt gctcgccgac
     1021 cagataaaga cgggtgaatt cgtcgtgccc gatccctata tgcttctcga agagctctac
     1081 acaatgatcc agaacaccaa cgtggaacac acctacttcg cgtcgaacca cgcgtcgaac
     1141 tacctccccg ttaaggcctg gctccccgag gagaaagaga agacactcag ggccatacaa
     1201 cacgtcctcg ctgagcgcga tccttcgatg ctcagacccg agttcatgag ggccctgtga
     1261 tcatgccggg acaggcgaat gaccacgcaa gggacgacgg agggttcatc gatgtgccgg
     1321 tgcgcgtccg ctacgccgac accgacagga tgggcatcgt ttactacggg aattacccgg
     1381 ccttcttcga gatgggacgc agcgagtaca tgcggcaaaa aggctacccc tacagggacc
     1441 tggagaccct gggataccac ttcgtcgtgg tcggtctcga ggccaaatac tacaattcgg
     1501 caacctacga cgatatcctc atcgtgaaga cccgcgtgac ggaactcaag tcgcggggca
     1561 tcaccttcca ctacgtgata acgagggacg ggaccctggt cgtcgaggga tcgacaaagc
     1621 atatctgtgt gaataccgac aagaagcccg tcacgatacc acagaaactc atagaaatct
     1681 tccgagatgg cacagccgga tagcatcctc gtcatccgac tgagctccct cggtgatgtc
     1741 ctgatgagcg tgccagcggt aagggccttg aggcggcgct ttcccggggc gcacatctca
     1801 tggctcgccg aaggttccgt gacgggaatc ctcgcgcacc aggatttcat tgacgaggta
     1861 atattcttcc cccgggcggc cctcacgaaa cacctcagat cgggtcatct cgccacggag
     1921 gcttatattc tccgtgattt cctgaaggac cttagaaagc gggaatacga ctgcatcctc
     1981 gacctccacg gcattgcgaa aagcgtggcc cttatgaagc tggcgcgggg aaaaagacgg
     2041 atagggttcg gaaagacatt cgcaaaggac atgagccacc ttttctacgg cgaaagagtc
     2101 gagggaactg aaaagaggat acacaaggtg gaacgtaaca tgcttgccgc ccgttatctc
     2161 ggatgcgccg accctcccgg gaatgtcccc cttctcgtgc acgacagcgc ccggacatac
     2221 atagacgatt ttttgagatc acgcgacgtc gcgtcccccg tgatagccgt gaaccccttt
     2281 gccagctgag attccgcctt caaaagatgg cctctcgaac gttacgccga cctcatcggg
     2341 atgatacggc gggaacgcat gggcagcgtc gtaataatct ggggaccggg cgaacgggaa
     2401 gaggcccaaa ggcttgccgc gatggtgggg gacggggccc ggcttgcctg ccagacggac
     2461 atcgcacagc tctacgctct tctcgcgcag agcgccgttt acataggggg cgatacggga
     2521 acgacccatc tcgcgtccgc cgcgggcgtc cccgtcctgt gcctcttcgg ccccaccgac
     2581 ttcatcgtga accgccctta ctcgaaaaac agcgtcataa tacggaagga tgtctcctgc
     2641 agcccctgca aaaagaagga ttgcaggacc cgggaatgcc tcatgaccat atctccagac
     2701 gaggtcttcg cggcactgag gaagatgcct ttcaggaaag aggcttaaag gtatgcgctt
     2761 tctcttcgtc tatcccaaca tcaacaccca ggtggggttc aattacggca tctcctatat
     2821 ctcggggttc ctcaggtcga ggggcatcga gacggggctc ctcaacatca acgataagct
     2881 gggctatccc atggacatgg ataggatcag gcatgacgtc ctcgctttca agcccgacat
     2941 cataggcttc tccgtcctca ccaaccagca caagtacgcc ctcgatatag cccgcgatat
     3001 caagacgtac ctcgacgtgc ccatcctttt cggcggcatc cacgtcacga tggatcccat
     3061 ggaaacgctg atgcgcgatg aggtcgacct catatgcgtc ggcgagggag aagaggccgt
     3121 tgccgagctt gtcgagaaag gcgatccccg gggcattgcg aacatcggct ggaaggagaa
     3181 aggaaggccc gtcatcgaac cccttcggcc ctacaaggac atagcgagtc tgcccttcaa
     3241 ggactacgag atcttcgatt tccagagact gatagacgcg aaggacggat gggtgggact
     3301 caccgcgtca cggggctgtc ccttccgatg tacctattgc ctcaaccaca agatcataga
     3361 ggtctacaag gaaagtggtc atcgcccgcg gggttacatc agaagacaca ccgtggacca
     3421 gatgatctcc gagatcgagt acctcctcgg ccgctataca gggataaaga tgttcatttt
     3481 cgacgacgac atcttcacct tcgacaaggc ctggctcagg gagttcaccg agcggtacag
     3541 ggcggtcacg gacataagct tcgtctgcaa cgctcacgcc cgcattttcg acgaggaaac
     3601 ggccgagctc ctgaagggcg cgggatgccg catcgtcaag ttcgggctcg aaagcggcag
     3661 cgacaggata cgccgaaagg tcctgagccg ctatatgacg aacaaacaca tcgaggacgc
     3721 cttcgccgtc gcgcacagac acgggctcca cacgtcggcc ttcgtgatgc tcggtcttcc
     3781 ccacgagacg ataggggata tcaaacaaac catcgatctg atcgccgcca tcaagccggg
     3841 acgtttccgc tggtccctct tcttcccctt cgtgggaaca aaggcgtata acatcgccct
     3901 ttcctcggga caggtcgacc tcacggagat ggaaaggctc gacaacttca ccgacgagac
     3961 ctgcatgagg cttggccccg acgtggacct ttactgcgac aaggtcaaga gcgcgctctg
     4021 cgccttcgtg aacgctcact cagggctgga acacaccgaa ggcttccggc gcctcgcgga
     4081 agagatcgag aacatggacg agaagacctg gcgcgaaaaa aaggcgagta tcctcgacag
     4141 ggtggaagag ctcaaccagg aaatggaaaa atcgggcgga ctccattata ctgtgagata
     4201 caatcctttt atgggcgtgc gaagtgactg gaaagacgat agtatatctg cctaactggc
     4261 tgggcgacat ggtgatggcc gttcccttcc tccatgccct ccggcagcac tcacaggacg
     4321 aggtatgggc catggggaag gaaagcgcca tccacctgta caacggactc ggactcttcg
     4381 accgtttcgt tcccatcagc gggaaggggc tcggcctctt cttcgaaacg acgaacaggc
     4441 tcaacagggt gggcttcacc cgggccatcg ccctgcccca ttccttcagg tcgaccctcg
     4501 tcttcttcaa cctgagggtt cgcgacgtca taggatacgc acggaacagg cggggcttca
     4561 tgctgaacgt gaaggtcccc gaggcaaggg agcttcagtc cacagtcgaa cactacctca
     4621 ggatcgcgga cacgctcgga gtaccccgga cgatcgaggc acccgtcctt tccgtgtcgg
     4681 ccgacgagga gaagaagttc gacgaggagt tctccgacat gacgaggcct tacggcgtca
     4741 tgatagtggg cgcccagtac ggcccgtcga aatgctggcc tgccgggtat ttttccgagt
     4801 tcgccgatga gatcatcgaa aggtacggta tgaacgtcta catgcttccc ggcaggggcg
     4861 aggacgtcct ggcccggaag atccacgaag ggatacgcgc cggggagcgc gcccggatca
     4921 gatccatgaa catacgggac atgaaggtgt gcctgtcacg ggcggccttc gtcgtcagca
     4981 acgacaccgg cccgcgccac atctccgcgg cgctgtccgt cccgaccgtt gttcttgccg
     5041 ggcccatgga tgaacgttat acggcctatc cgggcggaca cacccgcgtg gtctcgaagg
     5101 acatggcctg ccgcccctgc aacaagaaaa aatgcgacgg gaaccatgaa tgcatgacag
     5161 gaataaagcc gcgggacatc gctgagatag tgggggatat ccttgaaaag agagtctgag
     5221 gacaagctga ggaaaccggg gcggaaggcg aaagaacggg acggggcggg gcttattcgc
     5281 tttctcgacc gttttcgcgg caagaagata tgtgtcgtgg gcgacatcat agccgacgtg
     5341 ttcatcctgg ggaagcccta ccgcctctcc agggaggccc ccgtggtcgt cgtcaagtac
     5401 gaggagtcga agatctatcc cgggagcgcc ggcaacacca tcaacaacct tgccgcgctc
     5461 ggggcgacgg tctatcccgt cggcttcgtg ggaagcgatt cgatgggcgg taagctcatg
     5521 gatttctttt ccacgaggaa gaccatcaac acggcgggaa tcatccgcgt cagcggtgac
     5581 acggtgacaa agacacggat cctcgccggg gacacccacg tctcgaaaca gcaggtcatc
     5641 cgcatagaca aggatgacgg ccgcaggcgc accacgaagg agaaggccct cctcatggaa
     5701 aggatcgccg ggatctgccc ccacgtggac gcggtgatcg tctccgacta cgggcacggc
     5761 gccgtcagcg gcaaggtgat cgaatacatg aagatcctcg ccaacgataa gatcgtcgtc
     5821 ggcgattcac gttacggcct gaagaagtat tcggggttca ccatgatcac ccccaacgag
     5881 tccgaggcat ttgccctctc aggcctcagg gagaacgaac cgatagagga cgcgggaggg
     5941 aaggtccttt cgggtatgcg cctttccgcc cttcttgtca cccgcggcaa caagggcatg
     6001 acgctctttc tcaaggacgg aaaggtccat cacatcccca tatcgggcaa ggatgacgtg
     6061 acggacgtga ccggggccgg cgacacggtc tgcgcggccg tcgctctctc cctggcgagc
     6121 ggggcgggct tcctcgatgc ctcgcggatc gcaaactact ccgcggggat cgttgtcatg
     6181 aaacggggga cggcgacggt taccgtggca gagctgaaaa ggtcgataga gaacggggga
     6241 aagatttgaa aacggttccg ggttcaaggg ttcaagagtt caagagttcg aggggccaaa
     6301 atgggatggg cgttcaatgg ggaagatagt caatggcctg gatgagctta aagcaatcat
     6361 cgagagggag aaggcggcgg ggaagagggt tgtgttcggg aacgggtgct ttgatatcgt
     6421 ccatgtaggg catgtgcgat acctcagggg ggcgaaggaa ctcggggacg tcctcgttgt
     6481 cgccgtcaat gacgacgcct ccgtcaccgg tctcggcaag cgcaaggagg tcgtcacgcc
     6541 ggcggccgag cgggcggaga tcatatcagc catcgactgc gtcgattacg tcatcctctt
     6601 cgccgacccc accgtggaaa acctcctcag ggaactcaaa ccccacatcc acgccaaggg
     6661 aaccgattac accgaaggaa atgtccccga gcgcgatatc gtcatgtcct acggcggaag
     6721 ggtcgccata gtcggcgacc ccaaggacca ttcgacgcgg gatatcataa aaacgataaa
     6781 gaacctgaga gaaaaatagc gggaagggtc cggatagaac aacccaacag gacctacagg
     6841 aaagatagga cctatctatc ccctgcccct tccttctcat ttgatttcaa acacttttta
     6901 gtgtattgtt acaccatcgg accacatgag aatggaaaga caacaggacg cgagaccacc
     6961 cctatccgtg tacatgatca ccttgaacaa cagcgccacc atcgcacggg cgctcgaaag
     7021 cgttgccgga tgggcggatg agatcatagt cgtcgattcc cacagctccg acggcactgc
     7081 cgacatcgcc tcaaaataca cggaacaggt cctgcagttc gataccacaa gtcaaccgga
     7141 aaagtatcag tacgcccagg accgctgcag gaaccgctgg gtcctcttca tcgatgccga
     7201 cgagtggctc acggaagact tcaggaaaga gacggagacc atactcgcct cgggttcggc
     7261 aagcgacggc tacatagccg accggcgcaa catctacctg gggcgggaga taaaacatgg
     7321 aggctggtac ccggaccggg agatacggct ctacaggaaa gaccggggcg gctgggaggg
     7381 cggcatacac gcgaaggttc acgtgaaggg agaggtgggg cacatgagga accactacct
     7441 ccacacgcct tacgcggatt cggcccatca gatccggacc atcgaccgct attccgaggc
     7501 ctacgcccgt gaccttctcg cggcggggcg ccgctttcac atggcaaata tgctgctccg
     7561 tcccatctgg cgcttcttca gggattacat cattaagatg ggtttcctcg acggcgtgcc
     7621 cggcatcatc atcatggcgt ccaccatgta ctacgtcttc atgaagcacg cccgattgtg
     7681 ggagatggag agagacgatg gaggaaagaa aggtgttcga taacaaagac gtccttgtca
     7741 ccggagggct cggcttcatc ggcagcaacc tctgcataga actcgtgagg ctcggcgcgc
     7801 gggtcaccat catcgacaac atgctcccgc gccagggcgg caatctcttc aacatacgcg
     7861 acattgccga cagggttacg gtcaacgtct ccgatgtccg caatcagctc tccatgaacc
     7921 acctcgtcaa ggggaaggac tacatcttcc acctggcggg ccaggtcaac cacgtggaca
     7981 gtatgaagaa ccccctgcag gatctggaga tcaactgcac ggggacgctc gtcctccttg
     8041 aggccctgcg ccacaacaac agggacgcca gggtcatctt cgccgggacg cggggcgagt
     8101 acggatcgag cgtcaggctg cccgtggacg aagaccaccc gacaaacccc aagggcatct
     8161 acgccgtcac gaacctgacg gcggagaaga tggtcctcgt ctatgacgac gtctacaaga
     8221 tacggggcgt gtgcctgcgc atcaccaaca cctacgggcc gcgccatcag atggcccacg
     8281 acgagtacgg ggtcttcaac tggttcatcc ggaaggccct cgacgacgag gagatacccg
     8341 tcttcggtga cggcaggata ctccgggact tcctctacgt cgaagacctc gtggactcga
     8401 ttctcaaggt ggccgccgcg gaggaggcct acgggaaggt cttcaacgtc ggtaccggcg
     8461 tccccgtcag tttcttcgag cttgccgaga agatcgtggc tatcgccggg acggggagaa
     8521 cgaggttcac c
//
LOCUS       DAOQTR010000019         8148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9756_length_8148_cov_3.636229, whole genome shotgun sequence.
ACCESSION   DAOQTR010000019 DAOQTR010000000
VERSION     DAOQTR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9756_length_8148_cov_3.636229"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="flgL"
                     /locus_tag="PKN85_00960"
     CDS             complement(<1..864)
                     /gene="flgL"
                     /locus_tag="PKN85_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNT43002.1"
                     /translation="MRISESMRYRLFQSCINSMGQQLTDIEKKISTQKKINAPSDDPL
                     IFARCVEYDSDLSVGTQFNDNLQRLKTLVGMYDTSMSSIDDQLATLGEMAAGYDSVDT
                     SLRQSYVDELQGIIEHLVTVGNTRLGNTYIFGGQQADSSPFRLNNDYSVTYMVSAGGE
                     DATNIYVDSSQLGQYGLSGREAFYSTTKIAFGNVSNAYQGDIYSNTGSFAYVLNAGNR
                     TMYLNGAAVNLTAGVYTGNTLAQEIQTRLGADYSVAFDSTTRKFVITNNTTGVVTFNW
                     SNAGATAAGVLG"
     gene            complement(861..2261)
                     /gene="flgK"
                     /locus_tag="PKN85_00965"
     CDS             complement(861..2261)
                     /gene="flgK"
                     /locus_tag="PKN85_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNT43003.1"
                     /translation="MGITSILNIARNALFAQQTSLQVLSNNIANVNTKGYARQEAVLT
                     EADAVMGDTGLLIGNGVRVDRVASHYDKYLEFTVARQYTALGEQKTYEKFFTRIESVL
                     DETNSKLTSNITAFFNSWQSLSADPLSTVARADVALKATNLCQSIRNTYAQLKNLQVE
                     TDNNVASEVTEINNLTASIAVLNQKTYEAAAGGSESASFTSQRLMALQELSGKLDLQY
                     FEDENGGLTVMTADGKLLVEKGISYELTAEKTGSDNFYHVYWKGNSANSVDITDGIGG
                     GTLKSLIDLRDTQFTSFIDDIDDLARSLMTEVNAIHATGYNPGGTTGVNFFENLTRDY
                     AANLDISDEIKADVNYIAVTSSASNLSGNDIALAIADLGTADVTIGGTTTTYVSYSAS
                     IASTIGNLSQNAQNLSEYHQNLLDMVSNQRESVSGVSIDEEMSNLIKFQYAYQAAARL
                     INVADTLMSALIGIGR"
     gene            complement(2281..2715)
                     /locus_tag="PKN85_00970"
     CDS             complement(2281..2715)
                     /locus_tag="PKN85_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNT43004.1"
                     /translation="MMTDAIIEMTKREEKTLKEFLTALQMERDAIISFSLDGIIAGNN
                     KKEEILRKLEYLKAEKEKLLEGAGDREAIAADETIRSLNGSLAVVMKEIKVAMDKNMK
                     LLSFSIDHVRSSIENIIGHISGNMGYGKKREDLSSVLLSKVI"
     gene            complement(2715..3029)
                     /gene="flgM"
                     /locus_tag="PKN85_00975"
     CDS             complement(2715..3029)
                     /gene="flgM"
                     /locus_tag="PKN85_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNT43005.1"
                     /translation="MKIQNGNKPDLLDSLVKTAQVKPQQQKDLSVEQRKGQGASYDKV
                     ELSSRKQEVDRLTEKAKTQSVVREEKVEELRARIEDGTYNARGQIVAGSILRSNILDE
                     LL"
     gene            complement(3042..3389)
                     /locus_tag="PKN85_00980"
     CDS             complement(3042..3389)
                     /locus_tag="PKN85_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43006.1"
                     /translation="MSESVKSIMAGLPPAISMGIGTMERDLSGSSEADKEKVCRSFES
                     FMIYSMIKHLEKTTKMSKKGYAEETYMAMVYEKVADFLADKGVGIKEMLMKYSDRENA
                     KVIPQSGDNTGKR"
     gene            complement(3403..4530)
                     /locus_tag="PKN85_00985"
     CDS             complement(3403..4530)
                     /locus_tag="PKN85_00985"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNT43007.1"
                     /translation="MSYRERVHRSTTIVCVLFFIAIFLLFMVGMADAARIKEMGYING
                     ARTNQLIGYGLVIGLAGTGDKSNTIFTNQSLANMLETMGIRVDPKTTKVNNVAAVMVT
                     CNLPPFARIGSKVDVTVSSIGDAKSIEGGVLVTTPLKGVDGQVYAMAQGSTVVGGFLA
                     SGQGASVQKNHPTVGRIVNGATIEREVISEDYKGSQVMIALKMPDFTNARRIADRINE
                     TFGGAAHAKDAGTVNVTIPPTMRNNPVQFLSVVENLDIKTDMQAKIVVDEKTGTVVIG
                     ENVRVSTVVISHGNISVQIKEDARVSQPMPFGQGQTVVTPDTQIKVREDRGKFYLLEA
                     GISIRELVNALNATGISTRDVITILQTIKASGALHADLEVI"
     gene            complement(4542..5243)
                     /locus_tag="PKN85_00990"
     CDS             complement(4542..5243)
                     /locus_tag="PKN85_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNT43008.1"
                     /translation="MKTSAVLLMVILGAVLLAGCQSTSQSVIKDEPFVIDNTYKNAKP
                     ALQPPAGTIYRGVNANSNFLGDHRARGVGDIVTVKIYEVTNASEKAGTKTAKSATTTA
                     GIPNFLGLESNFYPSSITPDKMINANTKNDFDGSGETTRGGSLTATITARVVDVLPNG
                     NLAIEGKREIVINNEKKEILVQGIVRPRDLDYDNSVYSTQVADAKIIYTGVGVLGEKQ
                     RPGWLARIMDTVWPF"
     gene            complement(5256..6017)
                     /gene="flgA"
                     /locus_tag="PKN85_00995"
     CDS             complement(5256..6017)
                     /gene="flgA"
                     /locus_tag="PKN85_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNT43009.1"
                     /translation="MDTPINTNRDGRMFRGPEAVRSLCVGLLILFFFIAAAVGSLRTV
                     HAAGESAAEARIIKFVKEIYPGGDAVRVRLTTIPAQLREKVKIVNLSFVRIPDVSGDG
                     VCSVEIETAPGRLRTVQVPFRVFTKRELYVLKEAGQKGDTIDPKDVVVRETYMNGKGL
                     GYPASIEDVVGKVLKRDVPANTLVTYQILEDRVVVRRGDTVTIIAETDKLVVRAKGKT
                     VDKGRVGDVIRVKNIASGKEVMAKVVNSSSVKVEF"
     gene            complement(6020..6808)
                     /gene="flgG"
                     /locus_tag="PKN85_01000"
     CDS             complement(6020..6808)
                     /gene="flgG"
                     /locus_tag="PKN85_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNT43010.1"
                     /translation="MIRALWTAGTGMNVQQTNLDVIANNIANVNTNGFKKSRADFQDL
                     MYQTLRLQGARTEGGGMVPTGIQIGLGAMLSSVQKVFQQGDYQRTDNELDIAIEGNGF
                     LQVNLPSGDKAYTRAGALKTDADGKIVTTDGYTIEPNLTIPARTTSISIESDGTVTVQ
                     IQGQSSPQQIGRLELANFINPTGLRAVGKNLFMESDASGSPTTGRPGENGMGTVLQGF
                     LEMSNVNVMQEMINMIIGQRAYEVNSKAIQAADEMLQMTNSLRR"
     gene            complement(6818..7534)
                     /locus_tag="PKN85_01005"
     CDS             complement(6818..7534)
                     /locus_tag="PKN85_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNT43011.1"
                     /translation="MTVAGKFVNEKRLDIIANNLANAQTAGFKASRPVFEMISTTQDE
                     TGREGLLKNAYVNLSDTYIDFSDASIVESGAKLDMAILGPGFFSVMTPEGVQYTRNGQ
                     FMLDKSSRLVTMSGDPVMGKGGEITINVTDGKDILIESDGSIYLGKDLVDTIKVVEFK
                     DMKGLKPVSRSNFAYSGNEAGETPALYSIRQGSYETSNVNIVREMVELIHTMRAYECY
                     TKIDQMFSDINGKLTELAKF"
     gene            complement(7922..>8148)
                     /locus_tag="PKN85_01010"
     CDS             complement(7922..>8148)
                     /locus_tag="PKN85_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43012.1"
                     /translation="NKIDFCIGRGISAVEMEVASFYAVARAKGLKAVAFLVVSDLFNN
                     NGTWTSGFHTRAFREGVKKLARFMNEKAVL"
BASE COUNT         1471 a         2386 c         2258 g         2033 t
ORIGIN      
        1 tccgaggacg ccggcggcgg tggcccccgc gttggaccag ttgaaggtca cgacgcctgt
       61 cgtattgttg gtgatgacga atttccgcgt cgtggaatca aaggccacgg agtagtcggc
      121 gccgagccgt gtctggatct cctgcgcgag tgtgtttccc gtgtagacac ccgcggtgag
      181 attgaccgcc gcgccgttca gatacatcgt gcggttcccc gcattgagaa cgtaggcaaa
      241 actgcccgta ttcgaataga tgtccccctg atacgcgttg ctcacgttgc cgaaggctat
      301 ctttgtggtg ctgtaaaagg cttcccggcc ggacagaccg tattgcccca gctgggagct
      361 gtcgacgtag atgttcgtcg cgtcctcgcc cccggcggac accatgtatg tcacggagta
      421 atcgttgttc aggcggaaag gggagctgtc ggcctgctgt cctccaaaaa tatacgtgtt
      481 ccccagtctc gtgttgccca cggtgacgag gtgctcgatt atgccctgca gttcatcgac
      541 ataggactgg cgcagactcg tgtccacgga gtcataaccc gcggccatct ctcccagggt
      601 ggcaagctgg tcatcaatgc tcgacatgga tgtatcgtac atgccgacaa gggtcttaag
      661 acgctgcaga ttgtcgttga actgggtgcc gacgctcaga tccgaatcat actcgacgca
      721 ccgggcaaag atgagggggt cgtccgaagg ggcattgatc ttcttttgcg tcgatatctt
      781 cttctcaatg tccgtaagct gctgtcccat gctgtttatg caggattgga acagcctgta
      841 ccgcatgctt tccgaaattc tcatcttccg atcccgatca gggcgctcat cagggtatcg
      901 gcgacattga tgaggcgcgc cgcggcctga taggcatact ggaactttat gagattcgac
      961 atctcctcat ctatggaaac gccggaaacg ctttcacgct ggttcgatac catgtccaga
     1021 aggttctgat gatactccga aaggttctgt gcattctgcg aaaggttgcc tatggtgctt
     1081 gcaatggatg cggaatagct gacataggtc gtggtcgtcc cgccgatcgt cacatcggcg
     1141 gtacccaggt cggcgatggc gagggcaata tcattgccgg acaggttcga tgccgacgag
     1201 gtcacggcta tataattcac gtccgccttg atctcgtcgc tgatgtcgag gttcgccgca
     1261 tagtcccgcg tcaagttctc aaagaagttg acccccgtgg tcccccccgg gttatagccg
     1321 gtagcgtgga tggcgttgac ctccgtcatg agtgaccgcg caagatcgtc gatgtcgtcg
     1381 atgaaggagg tgaactgggt gtccctgaga tcgatgaggc tcttcagcgt tcctccgcct
     1441 atgccatccg tgatgtcgac ggaattagcg gaattgccct tccagtagac gtggtagaaa
     1501 ttgtcgctgc ccgtcttctc ggctgtcagt tcgtatgata tccccttttc aacaaggagc
     1561 ttcccgtcag ccgtcatgac tgtcagcccg ccgttctcgt cctcgaaata ctgaaggtcc
     1621 agtttccccg aaagctcctg cagcgccatg agcctctggc tcgtgaaact cgcgctctcg
     1681 cttccaccgg ccgccgcttc ataggtcttc tggttcaaca ccgcgatgga ggcggtaagg
     1741 ttattgatct ctgtgacctc actggccacg ttgttgtcgg tctccacctg gaggttcttc
     1801 agctgggcat acgtattgcg gatactctgg cagagattgg tggctttcag cgccacgtcg
     1861 gccctcgcga ccgtgctcag aggatcggcg gacaggctct gccatgagtt gaaaaaggcc
     1921 gtgatattcg acgtaagctt ggaattggtc tcgtcgagaa cactctcgat ccgggtgaag
     1981 aacttctcgt atgtcttctg ttcccccagc gcggtatact gcctcgccac ggtgaattcc
     2041 aggtatttgt cgtagtggct cgcgacccgg tcgaccctca ccccgttgcc tatgagaagc
     2101 ccggtatcgc ccatcacggc gtcggcctcg gtgaggaccg cctcctggcg cgcgtaaccc
     2161 ttcgtgttca cattggcgat gttgttcgag aggacctgca gcgaggtctg ctgtgcaaag
     2221 agggcgtttc tagcgatatt gaggatagag gtgataccca ttgttaccct tcttcgtgcg
     2281 ttagatgacc ttagagagaa gtacggaaga gaggtcctct ctcttttttc cgtaacccat
     2341 gttgccgctt atgtgtccga taatgttctc tatcgacgat ctgacgtgat cgatggagaa
     2401 ggagagaagc ttcatgttct tgtccatggc gacctttatc tctttcatca ccacggccag
     2461 gctcccgttg agggaccgta tagtctcgtc ggccgcgatg gcctccctgt cccccgcacc
     2521 ctccagcagc ttttcctttt ccgccttcag gtactcgagc tttcttaaga tctcttcctt
     2581 cttgttgttc cccgcgatta tgccatccag tgaaaaggat atgatggcgt cgcgctccat
     2641 ctgaagggcc gtcagaaact ccttcagggt tttctcctct ctcttcgtca tttcgatgat
     2701 ggcgtccgtc atcattagag cagctcgtcc aggatattgg atctcagtat gctgccagca
     2761 actatctggc ctcgggcatt gtacgtcccg tcctcgatcc tggcccttaa ctcttcgacc
     2821 ttttcttccc ggaccacgga ctgggtcttg gccttctccg taagccggtc cacttcctgc
     2881 ttcctggatg aaagctccac cttgtcgtag cttgcgccct ggcccttcct ctgttccacg
     2941 gaaaggtctt tctgctgctg cggctttacc tgagcggtct tgacgaggct gtcaagcagg
     3001 tcgggtttgt tgccgttttg gatcttcatg gtccacctcc tctatcgctt accggtatta
     3061 tcgccgctct gaggaattac tttagcattt tccctgtcgg aatatttcat gagcatttct
     3121 tttatgccga cacctttatc cgcaaggaaa tcggcaactt tttcgtacac catggccatg
     3181 taggtttcct cggcataccc ctttttggac attttcgtcg tcttttccag gtgctttatc
     3241 atgctgtaga tcatgaagga ctcgaagctc cggcagacct tttccttgtc cgcctccgag
     3301 gaacctgaca gatcgcgttc catcgtgccg atccccatcg agattgcggg cggcaaacca
     3361 gccattatcg actttacgct ttcagacaca gtgagcggcc tcctatatga cctcaaggtc
     3421 ggcatgaaga gcgcctgacg ccttgatggt ctgcagtatc gttatgacgt ccctcgtcga
     3481 gatccccgtc gcgttgaggg cgttgacgag ttcccggatg gagatgcccg cctcgaggag
     3541 atagaacttc ccgcggtcct cacgcacctt tatttgcgta tccggcgtta ccaccgtctg
     3601 accctgaccg aacggcatgg gctggctcac gcgggcatct tccttgatct ggacgcttat
     3661 gttcccgtgg gagatgacga cagtggagac gcgcacgttc tcgccgatga cgaccgttcc
     3721 cgtcttctcg tcaaccacta tctttgcctg catgtccgtc ttgatgtcga ggttttccac
     3781 caccgacagg aattgcacgg ggttgttcct catggtcggc ggtatcgtca cgttcacggt
     3841 cccggcgtcc ttcgcatggg cggctccgcc gaaggtctcg ttgatgcggt cggctatcct
     3901 ccgggcattg gtgaagtcgg gcatcttcag ggcgatcatg acctggctcc ccttgtagtc
     3961 ctcggagatg acctcccgct cgatggtggc accgttcact atccggccca ccgtcggatg
     4021 gttcttctgc accgaggcac cctgacccga tgccagaaaa ccgccgacca ccgtcgaacc
     4081 ctgcgccatg gcatacacct gcccatcgac ccccttgagg ggcgtcgtca ccaggacgcc
     4141 tccttcaatg ctcttggcgt cgccgatgga agagacggtg acgtcgacct tgctccctat
     4201 ccgcgcgaag ggaggcaggt tgcaggtgac cataacggcg gcaacgttgt tcaccttcgt
     4261 cgttttgggg tcaaccctga tgcccatggt ctccagcata ttggcaaggg actggttcgt
     4321 gaagatcgtg ttcgacttgt cccccgtgcc ggcaagaccg atgacgagcc cgtagccgat
     4381 aagctggttg gtcctcgctc cgttgatgta gcccatctcc ttgatgcggg ccgcgtccgc
     4441 catcccaacc atgaagagaa ggaagatggc gatgaagaag aggacacaga ctatcgtcgt
     4501 gctgcgatgg acacgttccc ggtaactcat gccgtctccc gtcagaatgg ccatacggtg
     4561 tccatgatgc gtgccagcca gcctggcctc tgcttttcgc ccagtactcc cacgccggtg
     4621 tagatgatct tggcgtccgc gacctgggtc gagtacacgg aattgtcata atcgaggtcc
     4681 ctgggcctca cgatgccctg gaccaggatc tccttctttt cgttattgat gacgatctcc
     4741 ctcttccctt caatggccag gttgccattg ggaagcacgt cgacgacgcg cgccgtgatg
     4801 gtcgcggtca gagagccgcc ccgggtggtc tcgccggagc cgtcaaagtc gttcttggtg
     4861 ttggcgttta tcatcttgtc gggggtgatg ctcgagggat agaagttgga ctcgagcccc
     4921 aggaaattgg ggatgcccgc cgttgtcgtg gccgactttg ccgtcttcgt gcccgctttc
     4981 tccgatgcgt tcgtcacctc atagatcttg acggttacga tgtcacccac tccccgggcc
     5041 ctgtgatcgc ccaggaagtt gctgttcgcg ttgactccgc ggtaaatggt gcccgcgggg
     5101 ggctgcaaag ccggtttcgc gttcttgtat gtattgtcta tgacgaaggg ctcatccttt
     5161 atcaccgatt gactggtgct ctggcaacct gccagaagga ccgcccccag gatgaccatc
     5221 aacaatacgg cgctcgtctt cataaactca cactcctaga actcgacttt gaccgagctg
     5281 ctgtttacga ccttcgccat cacctctttc ccggaggcaa tgttcttaac ccggatcacg
     5341 tcgcccaccc gccccttgtc gacggtcttg cccttcgcgc gcacgacgag cttgtcggtc
     5401 tcggcgatta tggtcaccgt gtcaccccgc ctgacgacga cgcggtcctc gaggatctgg
     5461 taggtgacaa gcgtattggc cggcacgtcc ctcttgagga ccttgccgac aacatcttcg
     5521 atagacgccg ggtagccaag gcctttgcca ttcatgtagg tctcgcggac caccacgtcc
     5581 ttcggatcga tggtatcacc cttctgtcct gcctctttca gcacgtagag ttcccgcttc
     5641 gtaaagaccc tgaacggtac ctgcacggtg cgcagcctgc ccggagcggt ctcgatctcg
     5701 acagagcaga ctccatcgcc gctgacgtca ggtatgcgca caaagctgag gttgacgatc
     5761 ttcacctttt ccctcagctg ggcgggaatc gtcgtcagtc tgacccgaac ggcgtcgcct
     5821 ccgggataga tctccttgac gaacttgatt atcctggctt ccgccgcgga ttctccggcg
     5881 gcatggaccg tccttaagga cccgaccgcg gcggcgatga agaagaacag tatcagaaga
     5941 cccacgcaca aacttcttac cgcctccggc ccgcggaaca tccttccatc acggttcgtg
     6001 tttatcgggg tatccaccgc tatctcctca ggctgttggt catctgaagc atctcgtccg
     6061 ctgcctggat ggccttggag ttgacctcgt aggcgcgctg gccgatgatc atgttgatca
     6121 tttcctgcat gacgttgacg ttggacattt cgagaaaacc ctggagcacc gtgcccatgc
     6181 cattctctcc aggcctgccc gtggtgggcg aaccgctcgc gtcgctttcc atgaagaggt
     6241 tcttgcctac ggcccgaagc cccgtggggt tgatgaagtt ggcgagctcg aggcggccta
     6301 tctgctgggg agaggactga ccctggatct ggaccgtcac ggttccgtcg ctttctatgg
     6361 atatgctcgt cgtcctggcc ggtatggtga ggttcggctc gatggtgtaa ccgtcggtcg
     6421 tcactatctt gccgtcggcg tcggtcttga gggcgcccgc cctcgtatag gccttgtcgc
     6481 cggagggcag gttaacctgg aggaaaccgt tgccttctat ggctatgtcg agctcattgt
     6541 cggtcctctg gtaatccccc tgctggaaga ccttctgcac tgacgagagc atggcgccga
     6601 ggcctatctg gatacccgtg ggaaccattc cgcccccttc cgtcctcgcg ccctgcagtc
     6661 tcagggtctg atacatgagg tcctggaaat cggcacggct cttcttgaag ccgttcgtgt
     6721 tgacgttggc gatgttgttg gcgatgacgt cgaggttcgt ctgctgcacg ttcatgcctg
     6781 ttcccgctgt ccataacgcc cgtatcatgt gttcctccta aaatttcgcc agctctgtca
     6841 gcttgccgtt gatgtcagag aacatctggt ctatcttggt gtagcactcg taggccctca
     6901 tggtgtggat gagctccacc atctcgcgga cgatgttcac gttcgaggtc tcgtaggagc
     6961 cctgccgtat ggagtacagt gccggcgttt cccccgcctc attgccggag taggcaaagt
     7021 tgctcctgct cacgggcttc aggcccttca tgtccttgaa ctcgacgacc ttgatggtgt
     7081 ccacgaggtc ctttcccagg tatatggaac cgtcgctctc gatgagtata tccttgccgt
     7141 cggtgacatt gatggtgatc tctcccccct tgcccatgac ggggtctccg ctcatcgtca
     7201 cgagtcggct cgacttgtcg agcatgaact gcccgttacg cgtgtactgg acaccctccg
     7261 gcgtcatcac ggagaaaaac ccggggccaa ggatggccat atccagtttg gcgccactct
     7321 cgactatgga agcgtcagaa aaatcgatat aggtgtcgga aaggttgaca taggcatttt
     7381 tcaggagccc ctcccgaccc gtctcgtcct gcgttgtgga gatcatctcg aaaacgggcc
     7441 gcgaagcctt gaacccggcg gtttgagcgt tggccaggtt gttggcgatg atgtccagcc
     7501 tcttctcatt gacaaacttg ccggcgactg tcacgtaaaa gctcatgccg gggatagtgc
     7561 aatacacgtg ccagaaaaga ccgcttgaac ggcttgagcc gtttgaaccg cttgaacgga
     7621 aaggcggcag ggaacgaaga gacggtttga atcgcttgag tggcttgaat cgcttgacag
     7681 ctgaaaggcc ttgttccggc gaagggggtc gaggtccgag gctggaatgg gggatcttcc
     7741 cgtcgtccgg caggatcgga acccttacac ctgtttttga cccgcaaccc ggaacgcgca
     7801 acccgcaaca gtattctctt tgaaacctga ggcctgtgat gcttttttcc ttttgctggg
     7861 aaaaattttc cttttgggaa attttttcag ggaaaaaggc ggttcccggg gggtgtggcg
     7921 gtcagaggac cgctttttcg ttcatgaagc gggcaagctt ttttacgcct tccctgaagg
     7981 ctctggtgtg gaagccgctt gtccatgtac cattgttatt aaagaggtcg gagacgacga
     8041 ggaaggcgac ggccttcagg ccctttgccc tggcgacggc gtagaaagac gccacttcca
     8101 tctccacggc cgatatgccc cgtccgatgc agaagtcgat cttgttcc
//
LOCUS       DAOQTR010000020         8041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9917_length_8041_cov_3.849236, whole genome shotgun sequence.
ACCESSION   DAOQTR010000020 DAOQTR010000000
VERSION     DAOQTR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9917_length_8041_cov_3.849236"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1103
                     /locus_tag="PKN85_01015"
     CDS             165..1103
                     /locus_tag="PKN85_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin family protein"
                     /protein_id="HNT43013.1"
                     /translation="MTSWKGFVLFTICTLAFLMVLTVPLNAQRMGPRSGATMVKQTAG
                     NTRTVTFVNNCKVDVWWAVITSDKGKDCGSGFFAMPGNGGDKLAKQGGRQTITVNLEK
                     GCGNNMVWSGNFYPRTGCDFKNTNVGLCETGGCGDQLHCKAGVGGAPPKGLAEFRFDQ
                     NGNDFYDLSFVDGFNLPIQIKPTAIIKKPRNDKYGCGVSACKFDFVQETSVKDETSCL
                     NDLKVRNGRGTLVGCTSACQAFDAGHADRMANSKYCCTGSYFGPVNRNDAGNCPSTSY
                     SDFFKKKCPDAYAWPQDDNNSTFSCVATAYEVTFCP"
     gene            1209..2534
                     /locus_tag="PKN85_01020"
     CDS             1209..2534
                     /locus_tag="PKN85_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43014.1"
                     /translation="MLTGCTSFKIIAAFLLISQVLCQSAAWAGPMTPPTIIAPGQSSG
                     TGATSAPSEPSGGAGSSTDSDKPLSNIKSGEQTVAAGAGTSETPLKAEASPLDFKISP
                     DGTVATILFDRESVQAMRDLISQLEQTGALGKVRGIVYSAGGFTPMLILMGEKEELHQ
                     KLLLLKRFIPDQNQAHMIVISASLRELSDDDAMNVGLTLGPDIIGATISSSAGMLLQS
                     SESAQYSFSGSADLNAVSFAKIAQLNQALDRSKVLVSSEVYTRNGHKALLTNVQQVPI
                     FSTDFNNNVMTSYQQLETSVDVIPTTIDYRKDKPDESQVRVDVLVKISVVTANHTYNA
                     STTAPEYTTKTFATTRVLKANNARYVVGTFVNDAEYKEQVGLPLLSKIPIIKYLFSRE
                     GTRMVRNVAILTLAVRLIPMQVKDLTIDVEHVNPLEELYKRKGSLKDAK"
     gene            2947..4662
                     /gene="thrS"
                     /locus_tag="PKN85_01025"
     CDS             2947..4662
                     /gene="thrS"
                     /locus_tag="PKN85_01025"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNT43015.1"
                     /translation="MDENLDMIRHSASHLMAQAVRDLYPGVRVAIGPSIETGFYYDFD
                     YEPGFTEEDLPKIEKRMHELAAEDIPIERKVVRRQDAMAMFKEMGEPYKVELLEAIGD
                     DEVTTYTQGSFTDLCRGPHLASTGKIKAFKLLSLAGAYWRGDERNKMLTRIYGTAFAD
                     EKALKEYLTFLEEVKKRDHRRIGKDLDLFSISDEVGAGLVIYHPKGALLRWLLEDFER
                     REHLKRGYQFVVGPHILKMDMWKKSGHFENYRENMYFTKVDEVEYGIKPMNCLSHIMV
                     YKSQVRSYRELPLRYFELGTVCRHEKSGVLHGLMRVREFTQDDAHIFLRPDQLHEEIL
                     AIIRFVQDVMAIFGFQFEMEISTRPSKSIGSDEDWERAEKALKEVLDTEGLPYDLNEG
                     DGAFYGPKIDIKLKDALGRKWQCATIQCDFALPERFDLHYTDSDGIRKRPVMLHRVVL
                     GALERFMGVLIEHYGGKFPVWLAPVQVVVMNITDDQAPYVKNTANTLAAEGIRVETDL
                     RNEKLSLKIRESVVKKIPYMVIAGKKEMESNTLTVRVRDGGELKDIGLQDFLSRVKDE
                     NITRR"
     gene            4659..5186
                     /gene="infC"
                     /locus_tag="PKN85_01030"
     CDS             4659..5186
                     /gene="infC"
                     /locus_tag="PKN85_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNT43016.1"
                     /translation="MSLIAGKDFRDININERIRVREVRLIDENGEQLGIVPTVDALRL
                     ARERELDLVEVAGKSSPPVCKIMDYGKYKYQLSKKAQEAKKKQSVIQIKEMKLGLKIE
                     EHDFQFKMKHLRGFLEEGHKLKIIIMFRGREILHVDMGEKLAQKVVDSLKDVGELEQR
                     SRFDGRNIVMIFAPL"
     gene            5205..5402
                     /gene="rpmI"
                     /locus_tag="PKN85_01035"
     CDS             5205..5402
                     /gene="rpmI"
                     /locus_tag="PKN85_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNT43017.1"
                     /translation="MPKIKTHRGLAKRVKVTAGGKIKRAKAFHSHLLSSKTPKMKRRL
                     SKADTVHPADAKRIKSLIPYS"
     gene            5428..5787
                     /gene="rplT"
                     /locus_tag="PKN85_01040"
     CDS             5428..5787
                     /gene="rplT"
                     /locus_tag="PKN85_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNT43018.1"
                     /translation="MPRAKRGFKARQRRKKIFKLAKGMTQRRKNVYSVAKRSVFKALK
                     YAYIGRKERKRDFRSLWIVRINAACRSYGIPYNRFINGLKVANIALNRKSLADMAVND
                     PAGFGEVVAKVKAVVTH"
     gene            5796..6806
                     /gene="pheS"
                     /locus_tag="PKN85_01045"
     CDS             5796..6806
                     /gene="pheS"
                     /locus_tag="PKN85_01045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNT43019.1"
                     /translation="MDISGLKEKIEGEISRIGNIEDLKQVKVSLLGRKGVVADYLDKL
                     KTLDKDQRRDAGKAINEIKTWADLKIGELEKHFNEIERQNRERASLIDITMPGTAPVF
                     GKKHPITLTLEEIIRIFGSLGFAVAEGPDIEAEYYNFEALNIPSDHPARDMQDTFYIR
                     PGVVLRTHTSPVQIRTMEAQLPPVKIIAPGCVYRCDNDISHTPMFHQVEGLMVDKNVR
                     FSDLKGILSIFIHEMFGPDTPLRFRPSYFPFTEPSAEVDIGCVICRGAGCRVCKDTGW
                     LEILGSGMVHPQVFRNVGYDQEEITGFAFGMGVERIAMIKFGIDDIRQFYYNDIRFLS
                     QF"
     gene            6818..>8041
                     /gene="pheT"
                     /locus_tag="PKN85_01050"
     CDS             6818..>8041
                     /gene="pheT"
                     /locus_tag="PKN85_01050"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNT43020.1"
                     /translation="MKVPIEWLNEFVVIDIEPEELAKRLTMRGLEVEGVERLTPRFTG
                     VVAGTIARIEAHPNADNLSVCAIDTGREELTVVCGARNISVGDRVPLATVGATLDKDF
                     VVGKRKVRGIESFGMLCSEKELGLSDDHSGIFIMPDDIAAGSILADLPISHDIVLDVS
                     VPPNRGDCLSILGIAREVASILRQKAKLPGFELKAGSEGSVEDEIALHISDTMACPRY
                     VLKLIKGITIAPSPFWMRRRITRCGMRPINNIVDVTNYVMLELGQPLHAFDSERLDES
                     RIEVKVAGAPSTFRTLDSIDRPIEAGDILICDGSGPVAVAGIMGGENSEITDTTRNMA
                     LEAAYFNPYSIRATARRLGIRSEASLRFEKGIDIDNVGYAAERAMYLMHKLGGGQVVR
                     GVREIFEKKEPRTIYI"
BASE COUNT         1981 a         2297 c         2286 g         1477 t
ORIGIN      
        1 gctcagtggg agagcgctac cttgacgcgg tagaggtcag gggttcaata cccctagcgc
       61 ctaccatttt tgtatcttca tcacatcaca atatacacag ttggtgccga tggcagctga
      121 catcattttg cttctatctc tggaacaatg aggagggaat ttctatgaca tcatggaagg
      181 ggtttgttct tttcactatt tgcaccctcg cttttctgat ggtccttacc gttccgctta
      241 atgcccagcg catgggaccc cgaagcggcg cgacgatggt caagcaaacg gctggcaaca
      301 cgagaaccgt cacattcgtc aacaactgca aggtggatgt ctggtgggcc gtaataacga
      361 gcgacaaagg caaggactgc ggaagcggct tcttcgccat gcccggaaac ggcggcgaca
      421 agctcgccaa acagggcggc cgtcagacca tcaccgtcaa ccttgaaaag gggtgcggta
      481 acaacatggt ctggagcggc aacttctatc cccgcaccgg gtgcgatttc aagaacacca
      541 acgtcgggct ctgcgagacc ggcggatgcg gcgaccagct gcactgcaag gccggtgtgg
      601 gcggagcgcc tccgaagggc ctcgcggaat tccgcttcga tcagaacggc aatgatttct
      661 acgacctcag tttcgtcgac ggcttcaacc tgccgataca gatcaaaccc acggcgatca
      721 tcaagaaacc gcgcaacgac aagtacggct gtggggtctc ggcctgcaaa ttcgacttcg
      781 tccaggaaac cagcgttaaa gacgagacgt cttgtctcaa cgacctgaag gtccgcaacg
      841 gacgcgggac cctcgtgggc tgtaccagcg cctgccaggc attcgacgcg ggtcacgcgg
      901 acaggatggc caattcgaag tattgctgca cgggaagcta tttcggtccc gtgaaccgca
      961 acgatgcagg caactgcccg tccacatcgt attcggactt cttcaagaag aaatgccccg
     1021 acgcgtacgc ctggccgcag gatgacaaca acagtacctt ttcctgcgtg gccaccgcgt
     1081 atgaggtcac cttctgcccg taaggcggca ggcggccccg tgccggtgtt gcatggcatg
     1141 gcaaccgaca cgaaaccggc atcgagatgc cgatgccggt ttcgtgatcc tcaattaagg
     1201 aacacagtgt gctgactggg tgcacatctt tcaagatcat cgcggcgttt ctgctgatct
     1261 cgcaggtcct gtgccaatcc gcggcctggg cggggccgat gacgccgccc accatcatcg
     1321 cacccgggca gtcttccggc acgggcgcga catctgctcc gtcggagcca tcgggcgggg
     1381 cagggtcgtc gacggattcg gataaaccgc tcagtaacat aaaaagcggc gaacagaccg
     1441 tagcggccgg tgccggcacc tccgaaactc cactgaaagc agaggcgagc cccctcgatt
     1501 tcaagatcag tcccgatgga accgtcgcca ccattctgtt cgacagggag agcgtgcagg
     1561 cgatgcgtga cctcatctcg cagctcgaac agaccggcgc cctcggcaag gtcaggggta
     1621 tcgtttactc cgccggcggc ttcactccca tgctcatcct catgggagaa aaggaggaac
     1681 tgcatcaaaa actgctcctc ctgaaacgct tcatacccga ccagaaccag gcgcatatga
     1741 tcgtcatatc agcgagtctc cgcgaactga gcgatgacga cgcgatgaac gtaggtctta
     1801 ccctgggtcc ggatatcatc ggcgcgacga tctcgagctc cgccgggatg ctcctgcagt
     1861 cctcggagtc ggcccagtat tccttctcgg ggtcggcgga cctcaatgcc gtctccttcg
     1921 cgaagatcgc ccagctcaac caggcccttg acaggagcaa ggtcctcgtc tccagcgagg
     1981 tctacacccg caacggccac aaggccctcc tgacaaacgt ccagcaggtc cccatcttca
     2041 gcaccgactt caacaacaac gtcatgacat cctaccaaca gctcgagacc agtgtggacg
     2101 tcataccgac cacgatcgac tacaggaagg acaaacccga cgaaagccag gtccgcgtcg
     2161 acgtgctcgt gaagatcagc gtcgtgacgg cgaaccacac ctacaacgcg tcgacaacgg
     2221 cccccgaata cacgacgaag acgttcgcca ccacgagggt cctcaaggcg aacaacgcgc
     2281 ggtacgtcgt cggcacattc gtgaacgatg ccgaatacaa ggagcaggtc ggccttcccc
     2341 tgctctcgaa gatacccatc ataaaatacc tcttttcccg cgaagggaca cggatggtgc
     2401 gcaacgtcgc catactcacc ctcgccgtgc ggctcatccc gatgcaggtc aaggacctga
     2461 cgatcgatgt cgagcacgtc aatcccctgg aagaactcta caagagaaaa ggcagcctga
     2521 aggacgcgaa gtaagagaag ccgttgcggg tttcgggctg caagttgcgt gtcaaaagcc
     2581 cgaaaggcgg gaagcaagga agaggtagtt gcggggcaaa agtggggggg tagataggac
     2641 ctataggaca gaataatagg atatggtgaa gttcgcagcg tgcaaccggg ccgtcctata
     2701 ggtcctaagc gtcctatagg tcttattggt cctgtcgaaa gccgcgctcc cctctctgcc
     2761 ctgcccccga tccctgcccc tgtcttctgc ccccgcccct tccccccgtg ccccatcatt
     2821 tttcctcatc attcattaac tataaaattg acaagagaaa cacaaaaatg ttaataatat
     2881 ctttctgttc aggcaatgtt gctcaggtct ggtgtaagag gaagagggta atcaaaggat
     2941 agcaaaatgg acgaaaacct cgacatgata cgtcacagcg cgtcgcacct gatggcgcag
     3001 gccgtcaggg acctctatcc cggggtccgc gtcgccatcg gcccctcgat agaaacggga
     3061 ttctactacg attttgacta cgagccgggc ttcacggaag aagacctgcc gaagatcgaa
     3121 aagcgcatgc acgagctggc ggctgaagat atccccattg aacgcaaggt cgtcaggagg
     3181 caggatgcca tggccatgtt caaggaaatg ggtgaacctt acaaggtgga gcttctcgag
     3241 gccatcggcg acgacgaggt gaccacatac acccagggtt ccttcacgga cctctgccgg
     3301 ggtcctcatc tggcctccac gggcaagatc aaggccttca agctcctgag cctggccggg
     3361 gcatactgga gaggcgatga acggaacaag atgctgaccc gcatctacgg taccgctttc
     3421 gccgacgaaa aggcactcaa ggaatatctt accttcctcg aagaggtgaa aaagagagac
     3481 cacaggagga tcgggaagga cctcgacctc ttcagcatat ccgatgaggt cggcgccggg
     3541 ctcgtcatct atcatcccaa gggcgccctt cttcgatggc tcctcgagga tttcgaacgc
     3601 cgggaacacc tgaaaagggg ttatcagttc gtcgtaggcc cccacatact gaaaatggac
     3661 atgtggaaga agtcgggtca tttcgagaac taccgcgaga atatgtactt cacaaaggtc
     3721 gacgaggtgg aatacggcat caagccgatg aactgcctct cccacatcat ggtctacaag
     3781 tcgcaggtgc gaagctaccg cgagctgccg ctgagatact ttgagctcgg gaccgtctgt
     3841 cgccacgaaa agtccggcgt cctccacggt ctcatgaggg tcagggagtt cacccaggac
     3901 gatgcccaca tcttccttcg tcccgatcag ctccacgagg agatcctggc catcatccgc
     3961 ttcgtgcagg acgtcatggc catcttcggt ttccagttcg agatggagat aagcacgaga
     4021 ccgtccaaat ccatcgggag tgacgaggat tgggaaaggg ccgagaaggc cctcaaggaa
     4081 gtgctcgaca cagagggtct gccctacgac ctcaatgagg gcgacggagc cttctatggc
     4141 ccgaagatcg acataaagct gaaggacgcc ctgggaagga agtggcagtg tgcaaccatc
     4201 caatgcgatt tcgccctccc ggaacgtttc gatctccatt acaccgactc ggacggtatc
     4261 aggaaaaggc ccgtcatgct ccaccgtgtc gtcctgggcg cgctggagag attcatgggc
     4321 gttctcatag agcactacgg aggtaaattc cccgtctggc tcgcacccgt gcaggtcgtg
     4381 gtcatgaaca ttaccgacga tcaggccccg tacgtgaaaa acacggccaa taccctcgct
     4441 gcagaaggta tccgggtcga aacagacctg aggaacgaaa aactcagttt aaaaatccgc
     4501 gaaagtgtgg taaagaaaat cccctacatg gtaattgcgg gcaagaagga gatggagagc
     4561 aacactctca ccgtccgcgt acgcgacggc ggcgagctca aagatatcgg gcttcaagat
     4621 ttcctaagcc gggtaaagga tgagaatata accaggaggt gagccttata gcaggaaaag
     4681 acttcagaga catcaacatc aatgaaagga tccgggttcg cgaggtacgg cttatcgacg
     4741 aaaacgggga acaactgggc atcgttccca cggttgacgc cttgaggctc gcaagggaac
     4801 gggaacttga ccttgtggag gtggcgggga aatcctcccc gccggtctgc aagatcatgg
     4861 attacgggaa atacaagtat cagctttcaa aaaaggccca ggaagcgaag aagaaacaga
     4921 gcgtgatcca gatcaaggag atgaaactgg ggctcaagat cgaggaacac gacttccagt
     4981 tcaagatgaa acacctcagg gggtttctcg aagagggcca caagttgaag atcatcatca
     5041 tgttcagggg acgggagatc ctccacgttg acatgggtga gaagctggcg caaaaggttg
     5101 tagattcatt gaaagacgtg ggggaactgg agcaaaggtc cagatttgac ggcaggaaca
     5161 tcgtgatgat ctttgccccc ctgtgatcac ttaaggaggt tcagatgcca aagataaaaa
     5221 cccatcgcgg gttggcgaag cgcgtgaagg tcaccgccgg cggcaagata aaacgcgcca
     5281 aggccttcca cagccatctg ttgtcttcga aaacaccgaa gatgaaaaga cggctctcaa
     5341 aggccgacac cgtccatccg gcggacgcaa agaggatcaa gtcactgata ccgtattcat
     5401 agaaaggttc aagagaggta caagaccatg ccaagggcaa aacgaggatt caaggcaaga
     5461 cagagaagaa agaagatatt caagcttgcg aagggcatga cgcagcgcag gaaaaacgtg
     5521 tacagcgtgg caaaaaggag cgtcttcaag gccctcaaat acgcctacat cgggcgcaag
     5581 gaacggaaac gggacttcag gtccctgtgg atcgtccgca tcaacgcggc ctgccgttcc
     5641 tacggcatac cctacaaccg ctttatcaac ggtctgaagg tcgcgaacat cgccctcaac
     5701 cggaaatcac tcgccgacat ggccgtcaat gaccccgcgg gattcggcga agtggtggca
     5761 aaggtcaagg cagtggtcac gcactagggt gacgcttgga cataagcgga ctgaaagaga
     5821 agatagaagg cgaaatatcc cgtatcggga acatcgagga cctcaagcag gtgaaggtat
     5881 cccttctcgg gagaaaaggg gttgtcgccg actacctcga taagctcaaa accctcgaca
     5941 aggaccagcg gcgcgatgcc ggcaaggcca tcaacgagat caagacgtgg gcggacctga
     6001 agatcggcga actggaaaag cacttcaacg agattgaaag gcaaaacagg gaacgggcct
     6061 cgctcatcga tatcacaatg cccggcaccg ccccggtttt cgggaagaag caccctatca
     6121 cgctcacgct cgaagagatc ataaggatct tcggatccct cggtttcgcc gtggctgaag
     6181 gcccggatat cgaggcggaa tactacaatt tcgaggccct caatatcccc tcggaccatc
     6241 cggcccggga catgcaggac acgttctaca taagacccgg tgtcgtcctg aggacccaca
     6301 cctcgccggt gcagatacgc accatggagg cgcagctgcc gccggtcaag atcatcgccc
     6361 ccggctgcgt ttaccgctgc gacaacgaca tctcccacac gccgatgttc catcaggtcg
     6421 agggattgat ggtggacaag aacgtccgtt tctccgatct caaggggatc ctgagcatat
     6481 tcatccacga gatgttcggg cccgacacac ccttgagatt tcggcccagc tattttccct
     6541 tcacggagcc ctccgccgaa gtggacatag gctgcgtgat atgccggggc gcgggctgcc
     6601 gggtatgcaa ggacacgggc tggctcgaga tactaggctc aggcatggtc cacccccagg
     6661 tcttccgcaa tgtcggttac gaccaggagg agatcacagg cttcgccttc ggcatggggg
     6721 tcgaacgcat cgccatgatc aaattcggca tcgacgatat ccgtcaattt tactataacg
     6781 acatcaggtt tctttcgcag ttctgaggtg agatcaggtg aaggtaccca tcgagtggct
     6841 gaacgaattc gttgtcattg atatagaacc cgaggagctc gcgaagcgcc ttactatgcg
     6901 cgggctggag gtggaaggcg tcgagaggct cacgccccgt ttcaccgggg tggtggcagg
     6961 caccatcgcc aggatcgaag cccaccccaa tgccgacaac ctttccgtct gtgccatcga
     7021 tacaggcagg gaagagctta ccgtcgtatg cggggcacgg aatatctccg tcggcgacag
     7081 ggttccgctg gcaaccgtgg gcgccaccct tgacaaggat ttcgttgtcg ggaaacgcaa
     7141 ggtgcgcggc atcgaatcct tcgggatgct ctgttccgaa aaagaactgg gtctttccga
     7201 cgatcacagc ggcatcttca tcatgcccga tgacatcgcc gccgggagca tccttgccga
     7261 tctccccatt tctcacgaca tagtgctcga cgtcagcgtc cccccgaacc gcggcgattg
     7321 cctgagcatc ctcgggatcg cgcgggaggt ggccagcata ctgcggcaga aggcgaagct
     7381 gcccggtttt gagctcaagg cagggtctga gggctccgtg gaagatgaga tagcccttca
     7441 catatcggac acgatggcct gcccccgcta cgtcctcaag ctcatcaagg ggataaccat
     7501 agccccttcc cctttctgga tgaggcgacg gatcacgcga tgcggcatga ggcccatcaa
     7561 caacatagtg gacgtgacca actacgtcat gctcgaactc ggacaaccgc tccacgcctt
     7621 cgactccgag cgcctcgacg agtcgaggat agaggtgaag gtggccggag cgccgtcaac
     7681 gttccgcacc ctggacagca tcgaccggcc catagaggcc ggagacatcc tcatctgcga
     7741 cggctcggga cccgtggcgg tcgcgggcat catgggcggc gagaattccg agatcacgga
     7801 cacgacgagg aacatggccc tcgaagccgc ctatttcaat ccttactcca tacgtgcaac
     7861 cgccaggagg ctcgggatca ggtcagaggc ttccctgagg ttcgagaagg gcatcgatat
     7921 cgacaatgtc gggtatgccg ccgagagggc gatgtacctc atgcacaagc ttggcggcgg
     7981 ccaggtggtg aggggagtca gagagatctt cgagaagaag gagccccgga cgatctacat
     8041 c
//
LOCUS       DAOQTR010000021         8027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9937_length_8027_cov_4.145509, whole genome shotgun sequence.
ACCESSION   DAOQTR010000021 DAOQTR010000000
VERSION     DAOQTR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9937_length_8027_cov_4.145509"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1000)
                     /locus_tag="PKN85_01055"
     CDS             complement(104..1000)
                     /locus_tag="PKN85_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43021.1"
                     /translation="MTRQRKKARNAAADRTKDLEIALQEERSLSRARSTLLETNIKEL
                     NDVYAVLNEKLKALRVREERIKRFEGELVRANKLSSLGELAGSIAHEIKNPLISVQGF
                     ARRIAKTGDIARIRRYAGLIDQEAGRMANVLVKLLDFSRMDEPRREECDLHDIVDDTV
                     LFMEHHLTRFKNVTVDVRRGASPAIVRVDRIHIQQALVNLINNAAQAMPKGGVIRISS
                     GTDKGLGFITVADEGEGIGKDLLQKIFEPFFTTKPKGEGTGLGLSLSKRLLEANGGSI
                     DVETHPGEGSTFRLLIPLSKTA"
     gene            complement(1000..1449)
                     /locus_tag="PKN85_01060"
     CDS             complement(1000..1449)
                     /locus_tag="PKN85_01060"
                     /inference="COORDINATES: protein motif:HMM:NF022163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2507 domain-containing protein"
                     /protein_id="HNT43022.1"
                     /translation="MKDYLVDDVKNIVRPQLGEEVPLVLFRILRIIGMRSILGETSGA
                     TLYMMGKKVGNMLPARTMEEFSATIRQLKIGIPEVVIDDEDHMTVKLFECITCAGFTY
                     TGEMFCDMESGIIAGLLENVHGKKAKSTQTKSWSAGFNYCEFEVFLY"
     gene            complement(1507..2307)
                     /locus_tag="PKN85_01065"
     CDS             complement(1507..2307)
                     /locus_tag="PKN85_01065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNT43023.1"
                     /translation="MKDPLQTAVEMALASGRIQKDSLKENFAIHHKGEIDLLTDVDIA
                     CQEMIISVIKERFPEDDIIAEEKANLFEGDRNRWIVDPIDGTTNYAHGYPFFCTSIAY
                     EVNGVMVIGVVYSPIMDELFVAVRGAGAYLNGNRIRVSSVQRLKASLLSTGFPYDITT
                     NPDNNINHFIDFLYEAQAVRRDGSAALNLAYVACGRFDGFWEIHLNPWDLAAGALIVE
                     EAGGSLSSISGGTFSVYGGGVIASNGLSQEAMTGVIGKGPSGRNQRIP"
     gene            2394..3371
                     /locus_tag="PKN85_01070"
     CDS             2394..3371
                     /locus_tag="PKN85_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HNT43024.1"
                     /translation="MAELRRDPLTGDWVVVGYGGSKSSGTGVCPFCPGHESMTPPTIR
                     EYIDDRGDWLVRCFAASNPIFVIEVEEDRRGEGLYDKMGNVGAHEIIVENRSHSRTMS
                     MYGKRELQLVLNMYRDRILDLKGDTRFKHVQVFKNHGELAGSYLLHPHSHVLATPILP
                     ARASHEIVTTRNHFMQKERCLLCDIISQEIRQGKRLVSMNGSFVAICPFASRFPYETW
                     IVPRFHEANYENLVDQAMKDDLTEMLLDVMKRVEQFANAYTMEIHTSPNTTFAEPHGD
                     DLPLREYHHWHIEILPRDFRSSKYKRDDEFSVLSITPEEAAESMKAQRT"
     gene            3729..5816
                     /gene="nrdD"
                     /locus_tag="PKN85_01075"
     CDS             3729..5816
                     /gene="nrdD"
                     /locus_tag="PKN85_01075"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNT43025.1"
                     /translation="METTDLNLFVRTSEESIAGWDRSKIVDALIRETLIDRDTAGEIS
                     KEVEQMIRRSGIEVITAPLIRELVNARLIEKGLESSRKMHTRLGMPLYDVDSLILHPN
                     KENANVPHGPEATNLTLAEGIKKEYALLSVFSQEVADAHMNGDIHLHDLGFVDRPYCS
                     GQSLEYIKKFGLDLPHSLSMARPAKHAEVLLAHLVKFAAALQSHFAGAIGWDAINIFF
                     APYIEGMSDNDVKQLAQMLIFEFSQQAVARGGQAIFTDINIYWEIPKHFVNVPAIGPN
                     GKFTGKTYGEYEKEAQRFAWKLFEVYKEGDGAGRPFFFPKPIVHITEKFFHTDGHMDF
                     LNLICDVASDKGNTYYVFDRGETAKISECCRLSFKLEESDLLDAKEPWRMRYCALQNV
                     SLNLPRLAYMAKNDDTKLFNLITERFILAVKAHKEKRAFMEKLLSLGENGPLALLTMN
                     RDGMPYLRFNLASHLIGMVGLNEMVQIHTGEEMHESRRALKFGIKAIAHMNLLTEKMR
                     RQENMKIVLEQTPAESTSYRFARLDLRYFSPHAGRIMKGDIASGEVYYTNSTHLNVKH
                     AMNPIERVTTEGLFHPLIEAGSISHVWLGEAQPSKEAIADFVIKTFKHTTNDQIAFSP
                     EFTTCNKCHRTGRGLADTCSYCGSTDVDGITRITGYFTKVSSWNKGKLGELKDRYRNS
                     EFFSERRKAAAND"
     gene            5853..6116
                     /locus_tag="PKN85_01080"
     CDS             5853..6116
                     /locus_tag="PKN85_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNT43026.1"
                     /translation="MGIVKIFYKDDCPMCPMAKQLKDHLREKNVAVDDYNVGTTEGLA
                     EATFYRVMALPTILVEDESENSLGEWRGNVPHIDEVLSAVQGA"
     gene            6097..6876
                     /locus_tag="PKN85_01085"
     CDS             6097..6876
                     /locus_tag="PKN85_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNT43027.1"
                     /translation="MQFKEPDCPAVKGFIETSFVDWKGCLASVLFLGGCNFRCPFCHN
                     RDLVLNPGGLEDVPIEYIVATIRKYRAQWVDRIVVTGGEPTIHKNLYRLMGLLKSEGM
                     KIKLDTNGSSPSAVKGLVNDGLVDAIAMDVKGPLDRYSRWCGINVDDRKVQESIEFIM
                     GSGIDHYFRMTVVPFLHREEDVYMVAAHLQGAKDFVVQTFRPDITLNTAFSRIRPFSP
                     DRMNQIKANVSDIMAGESVRGKEVRPHLSIAGKTDQDNLYS"
     gene            complement(7033..>8027)
                     /gene="rpoD"
                     /locus_tag="PKN85_01090"
     CDS             complement(7033..>8027)
                     /gene="rpoD"
                     /locus_tag="PKN85_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNT43028.1"
                     /translation="LINSIKNQHEKKEEIRRNSVKVNEATKKLNEKKLKALEKKVEEN
                     LINLNLNKKVLEEIIRKVQRQLKFMDDKEARKVKKRLLEIGEIENGLKTVKNRLIQAN
                     LRLVINIAKKYLNRGLSFLDLIQEGNMGLMKAAEKYDYQKGYKFSTYSTWWIRQAITR
                     AIADYARTIRVPVHVLETMNKITKVTISLFQELGREPNLDEISLKAGLPLEKVRKIMK
                     VSNEPISIETPIGDDESKLGDFIADPKSPSPFNELVSISLKEEIDKVLSTLTPREEKV
                     IRMRLGIGEKTDYTLEEVGEVFGLTRERIRQIEAKALRKLKHPSRRKRLESFLE"
BASE COUNT         1754 a         2184 c         2238 g         1851 t
ORIGIN      
        1 ggaaagaacc gccgcatgag atcgttgatg tccattattg acatcagggg tcagttttca
       61 ggggtttctt ttgcgttcaa gcagcttaaa cggttcaagc tgttcaagcg gtctttgaga
      121 ggggtatgag gaggcggaag gtgctgcctt ctccggggtg tgtttccacg tcgatggagc
      181 cgccgttggc ctcgaggagt ctcttgctca gcgaaagccc gagtcccgtg ccttctcctt
      241 tgggtttcgt cgtgaagaag ggttcgaata tcttttgcag caggtctttg ccgatgccct
      301 caccctcatc tgcgacggtt atgaagccca ggcccttgtc ggttcccgat gagatcctga
      361 tcacgccacc tttcggcatg gcctgcgcgg cattgttgat gagattgacg agtgcctgct
      421 gaatgtggat cctgtcgacg cggacgatgg cgggggaggc gcctcgccgg acgtccacgg
      481 tcacgttctt gaagcgcgtg aggtggtgtt ccatgaaaag gacggtgtcg tcgacgatgt
      541 cgtggagatc gcattcctct cttctcggct cgtccatccg ggagaaatcg aggagtttca
      601 ccagcacgtt cgccatccgc ccggcttcct ggtctatcag cccggcataa cgcctgatcc
      661 tggcgatatc ccccgtcttc gcaatcctcc tggcaaaacc ctggacggaa atgaggggat
      721 tcttgatctc gtgggcgatg gagccggcga gttcacccag agaagaaagt ttgttcgccc
      781 tgacgagctc cccctcgaaa cgctttatcc gctcttcgcg gacacgaagg gccttgagtt
      841 tttcattgag gacggcatag acgtcgttga gctccttgat attcgtttca aggagagtgc
      901 tgcgcgccct ggaaaggctc ctttcttcct gcagggcgat ctccaggtct ttcgtccggt
      961 ccgcggcagc gttgcgcgcc tttttcctct gccgcgtcat cagtaaagga aaacctcgaa
     1021 ctcacaataa ttgaaacctg ccgaccagct cttggtctgt gtcgacttcg ccttctttcc
     1081 gtgaacgttc tcgaggagtc ccgcgatgat accgctctcc atgtcgcaga acatctcacc
     1141 cgtgtatgtg aaaccggcgc aggttatgca ttcgaaaagc ttcacagtca tgtggtcttc
     1201 gtcatcgatc accacttcgg ggatcccgat cttcagttgc cgtatcgtcg cggagaattc
     1261 ctccatggtc cgcgccggaa gcatgttccc gaccttcttt cccatcatgt acagggtggc
     1321 tcccgatgtt tcaccgagga tgcttcgcat gcctatgatc cgcaggatgc ggaacaggac
     1381 gagcggcact tcctccccca gctgcgggcg cactatgttc ttaacatcgt cgacaagata
     1441 atctttcatg tggtgtcctc cgccggtcct gcgccccccc gggggggttc gggtcttgct
     1501 gcgccgttac ggaatccttt ggttccgtcc ggaaggcccc ttcccgatga cgcctgtcat
     1561 ggcctcctgg ctgagaccgt ttgaagcgat gaccccgccc ccgtaaacgc tgaatgtccc
     1621 gcccgaaatg ctcgacagag agcccccggc ctcctcgacg ataagggctc ctgcggcgag
     1681 gtcccatggg ttgaggtgta tctcccagaa gccgtcgaaa cgcccgcatg ccacgtaagc
     1741 aaggttcagg gccgccgagc cgtctcttcg cacggcctgc gcctcgtaca ggaagtctat
     1801 gaaatgattt atgttattat cggggttggt ggttatgtcg taggggaacc cggtgctgag
     1861 cagcgatgcc ttgagacgct gaacggacga gacccgtatc ctgttgccgt tgagataggc
     1921 ccccgccccc cggaccgcga cgaaaagctc gtccatgatg gggctgtaga cgacgccgat
     1981 gaccatgacg ccgttcacct catacgcgat ggaagtgcag aagaacggat acccgtgggc
     2041 gtaattggtc gtgccgtcga tgggatccac tatccaccgg ttcctgtccc cttcgaagag
     2101 gtttgccttt tcctcggcga tgatatcgtc ttcgggaaaa cgctccttta tcaccgatat
     2161 gatcatttcc tggcaggcta tgtcgacatc ggtgaggagg tctatctcgc ccttatgatg
     2221 gatcgcgaag ttttccttga gggaatcctt ctgtatcctt cccgatgcga gcgccatctc
     2281 gacagccgtc tgcagagggt ctttcatact aggtaattct aatcgataat ttctttcatt
     2341 tgcaaggaaa cagtaaattg acaatgctcc ggttgtagtc tattatttcc caaatggcgg
     2401 aactcaggcg agaccctcta acgggagact gggtcgtcgt gggatatggc ggctcgaaat
     2461 caagcggaac gggtgtttgt cctttctgcc cgggtcacga gtcaatgacc ccgccgacca
     2521 ttcgtgagta catcgatgac cgcggcgatt ggctggtcag gtgctttgcc gcctccaatc
     2581 cgatcttcgt gatagaggtt gaggaggaca ggcgcggtga aggtctctac gacaagatgg
     2641 gcaacgtcgg tgcccacgag ataatcgtgg agaacaggtc ccattcgcgg acgatgtcca
     2701 tgtacggtaa gcgggagctg caactggtcc tcaacatgta ccgggaccgt atactcgatc
     2761 tcaagggaga caccaggttc aagcatgtgc aggtgttcaa gaaccatggg gagctggcgg
     2821 ggtcttatct cctccatccc cactcccatg tgctcgccac gccgatcctt ccggcccgcg
     2881 cttcccatga gatcgtcacg acacgcaacc atttcatgca gaaggagagg tgccttctct
     2941 gcgatatcat cagccaggag atacggcagg gcaagaggct tgtgagcatg aacgggagtt
     3001 ttgttgccat ctgtcccttt gcatccaggt tcccctacga aacgtggatc gtgccgagat
     3061 ttcatgaggc caattatgag aatcttgtcg atcaggcaat gaaggacgac ctgacggaga
     3121 tgctgctcga cgtaatgaag cgtgtcgagc agttcgccaa tgcctacacc atggagatac
     3181 acacgtcgcc gaacaccaca ttcgccgaac ctcacggaga cgaccttccc ctgagggaat
     3241 accaccactg gcacatcgag atcctgccga gggatttccg gtcgtcgaag tacaagaggg
     3301 atgatgagtt ctccgtgttg tccataactc ctgaagaagc ggccgagtcg atgaaggccc
     3361 agcgaacata gggtggatgc ctgtcatcgg agaacctgaa ggccccctat tgggatatgc
     3421 atatcctgtg cctcgtctgc aacggttgga atattcaggg aaaagattga gaaaattctt
     3481 gaagaaatac ttctatgtat aaaattttca aaaaatattg gcgagtggcc gcgctcaaaa
     3541 aaatcaaaaa aatctcttga caacgtgagg gccggtcagg taaaaagtac tacatgtagt
     3601 gtggatatct agtggacata ctatatgtag tgtttcggag ggggattgca ctacgattga
     3661 ctttcgggaa gcatagcctg tcttaacaag acgaatcata caagtaaaca attctgatag
     3721 ggggatgcat ggaaacaacg gatctgaatc tatttgtcag aacctcggag gaaagcattg
     3781 ccggttggga ccggtcgaag atcgttgacg ctctcatcag agagacgctg atcgaccgcg
     3841 acacggccgg cgagatcagc aaggaagtgg aacagatgat caggcggtcc ggcatcgagg
     3901 tcatcaccgc acccctcatc cgggaacttg tcaatgcgag actgatcgag aaggggcttg
     3961 aaagctcccg gaagatgcac acgaggcttg gtatgcccct gtatgatgtt gacagcctca
     4021 tccttcaccc caacaaggag aacgcaaacg tgcctcatgg cccggaggcc acgaacctga
     4081 ccctggcgga gggcatcaag aaggaatacg cgctcctgtc cgtcttctcc caggaggtgg
     4141 ccgatgccca catgaacggg gacatacacc tccacgacct cggttttgtc gacaggccat
     4201 actgcagcgg ccagtccctc gaatatatca aaaagttcgg gctcgatctg ccgcactccc
     4261 tgtccatggc caggccggct aagcatgcgg aagtgctcct tgcccatctc gtcaagtttg
     4321 cggcggccct ccagagtcat ttcgcgggcg ccataggctg ggacgccata aacatcttct
     4381 tcgcgcccta tatcgagggg atgagcgaca acgacgtgaa gcagctcgcg cagatgctta
     4441 tcttcgaatt ctcccagcag gccgtggcta ggggcggcca ggcgatcttc accgacataa
     4501 acatctactg ggagataccg aagcactttg tcaacgtgcc ggccatcggc cccaacggca
     4561 agtttacggg caagacctac ggtgaatatg agaaggaagc ccagcgcttt gcctggaagc
     4621 tcttcgaggt ctacaaggaa ggcgacgggg cggggcggcc cttctttttc ccgaaaccca
     4681 tcgtccatat cacggagaag ttcttccata ccgacggcca catggatttc ctgaacctca
     4741 tatgcgacgt cgcatccgac aagggcaaca cctactatgt cttcgacagg ggagagacgg
     4801 ccaagatatc cgaatgctgc cgcctgagct tcaagctcga ggaaagtgac cttcttgacg
     4861 cgaaggagcc gtggaggatg cggtactgcg ccctccagaa cgtgtccctc aacctgccga
     4921 ggcttgccta catggcgaag aacgacgaca cgaagctttt caacctcatc acggagaggt
     4981 tcatccttgc cgtcaaggcc cacaaggaga aacgggcgtt catggagaaa cttctttccc
     5041 tgggggagaa cggaccgctc gcgctcctca cgatgaaccg ggacgggatg ccctatctca
     5101 ggttcaatct cgcatcgcac ctcataggca tggtcggtct caacgagatg gtgcagatac
     5161 ataccggcga ggagatgcac gaatcaagac gggcgttgaa gttcggcatc aaggccattg
     5221 cccacatgaa ccttctcacg gagaagatgc ggcgacagga gaacatgaag atcgtcctgg
     5281 agcagacccc ggcggaaagc acgagttacc ggttcgcgcg cctggacctc cggtatttct
     5341 ctccccacgc cggcaggatc atgaaaggcg acatcgcatc cggtgaggtc tattacacga
     5401 attcgacgca tctgaacgtc aagcacgcca tgaatcccat cgaaagggta accacggagg
     5461 ggctgttcca tccccttatc gaggcaggct ccatatcgca cgtctggctt ggagaagcgc
     5521 agccctcgaa ggaggccatc gccgacttcg tcatcaagac cttcaagcac acgaccaatg
     5581 accagatagc tttctccccg gaattcacca catgtaacaa gtgccacagg acggggcggg
     5641 gtcttgccga tacctgttcg tactgcggtt ccaccgatgt ggatggtatt acgaggataa
     5701 ccggatactt caccaaggta tcaagctgga acaaaggtaa gctgggagag ctaaaggacc
     5761 gttatcgcaa ttcggagttc ttctccgaaa ggcgcaaggc ggctgccaac gactagaaca
     5821 ctgacggatc ctacaaaatt caaggaggga gtatggggat agtgaagatc ttctacaagg
     5881 atgattgccc gatgtgtcca atggcgaaac agctgaagga ccatctccgg gaaaagaacg
     5941 ttgccgttga cgactacaac gtcggaacga ctgaaggcct tgccgaggcg acattctatc
     6001 gcgtcatggc attgcccacc atcctcgttg aagatgagtc ggagaactcc ctgggcgagt
     6061 ggagaggcaa cgtgcctcat atcgacgagg tactcagtgc agttcaagga gcctgattgc
     6121 ccggctgtaa agggtttcat tgagaccagt tttgtggact ggaaggggtg tctcgcatcc
     6181 gttctcttcc ttggcggctg caacttccgg tgtcccttct gtcacaaccg cgacctcgtt
     6241 ctcaacccgg gggggctgga ggacgtcccg atcgagtata ttgtcgcaac cataaggaaa
     6301 tacagggctc agtgggtcga caggatcgtg gtgaccgggg gtgagccgac aatacacaag
     6361 aacctgtacc ggcttatggg tctcttgaaa agcgaaggga tgaagatcaa gctcgatacg
     6421 aacgggtcaa gcccctctgc cgtgaagggc cttgtcaatg acggcctcgt ggacgcgatc
     6481 gccatggatg tcaaaggtcc cctggaccgg tattcccggt ggtgcggtat caatgtcgat
     6541 gaccgcaaag tgcaggaaag tattgagttt atcatgggtt ccgggatcga tcattacttc
     6601 cgcatgacag ttgtgccttt tctccacagg gaggaggatg tctacatggt tgctgcccat
     6661 cttcaggggg cgaaagactt cgtggtccag actttcaggc ccgatatcac tctgaatacc
     6721 gccttttcgc ggataaggcc tttcagccct gacaggatga accagatcaa ggcgaacgtc
     6781 tcggacatta tggccgggga gagtgtccgg ggaaaggaag tgaggccgca tctcagcatt
     6841 gccggcaaga cggaccagga caacctgtat agttgaaagc agagaaccgg gggatcgatg
     6901 cccggcatgt ccgtggatga cagaaagagt aaatgaaaag gcaaaagatt cgatcttttg
     6961 cctttatttt ctatccggcc taattatttc caggtctctt ccttggatcc tgttctctgt
     7021 tccccacctt tcctattcca ggaaactttc cagtctcttc cgccgtgagg ggtgtttcag
     7081 cttgcgcagg gcctttgcct ctatctggcg gatacgctcg cgggtcagcc cgaagacctc
     7141 tccgacctct tcgagcgtgt agtccgtctt ctcgccgatg ccgagcctca tccggatgac
     7201 cttctcctcc cgaggggtca gcgtggacag gaccttgtcg atctcctcct tgagagagat
     7261 gctgacaagc tcgttgaagg gcgacggtga ctttggatcc gcaatgaaat cgccaagctt
     7321 tgattcgtca tcgccaatgg gggtttctat ggagatcggc tcgttcgaga ccttcatgat
     7381 cttccgcacc ttttccaggg gaagccccgc cttcagcgag atctcgtcga gattgggctc
     7441 ccggccgagt tcctggaaga gggagatggt aaccttcgtt atcttgttca tggtctcgag
     7501 gacatgaacg ggaaccctga tggtgcgcgc gtagtcggcg atggcacggg tgatggcctg
     7561 ccggatccac caggtcgagt aggtggagaa cttgtatccc ttctggtaat cgtacttttc
     7621 ggctgccttc atgagaccca tgttcccttc ctgaatgaga tcaaggaacg aaaggcccct
     7681 gttaagatat ttcttggcta tattgatgac aagcctcagg tttgcctgta tcaggcggtt
     7741 cttgaccgtt ttgaggccgt tctcgatctc accgatctcg agcagtctct tcttgacctt
     7801 ccgcgcttcc ttgtcgtcca tgaacttgag ctgtctctgg accttgcgga tgatctcttc
     7861 aaggaccttc ttgttgagat tgaggttgat gaggttttcc tcgaccttct tttccagggc
     7921 cttgagcttc ttttcgttga gctttttcgt tgcctcgttg accttgacgg aattccttct
     7981 gatctcctct ttcttctcgt gctggttctt gatgctgttg ataaggg
//
LOCUS       DAOQTR010000022         8006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9971_length_8006_cov_3.684820, whole genome shotgun sequence.
ACCESSION   DAOQTR010000022 DAOQTR010000000
VERSION     DAOQTR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9971_length_8006_cov_3.684820"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKN85_01095"
     CDS             <1..987
                     /locus_tag="PKN85_01095"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNT43029.1"
                     /translation="IGLCRTEHMFFEGDRIDAVREMIIADDEAGRRKALAKLLPYQKK
                     DFVGLFTVMKGLPVTVRTLDPPLHEFLPHTERETRELARKMGISYEKVLAKVESLHEA
                     NPMLGHRGCRLGIVFPEITEMQARAIFEAACEVKKKGINVKPEVMIPLVGNVHELKLQ
                     KAIVEDVANEILKKNKVKIDYMIGTMIEIPRAAITADEIAQEAQFFSFGTNDLTQMTL
                     GMSRDDAGKFLSDYVKKEIYTFDPFQRIDEGGVGRLIELGVALGRGVRKNLKIGICGE
                     HGGEPNSVEFCHRTGFDYVSCSPFRVTIAKLAAARAALKEKQAKPKKAAKKK"
     gene            1231..7080
                     /locus_tag="PKN85_01100"
     CDS             1231..7080
                     /locus_tag="PKN85_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43030.1"
                     /translation="MKRFLSSLREDPEKTAAQCVQRLWQATGESPAEAGKCTALARLL
                     VDGLADSVEAMDPGRFMDSIDACRPDHATMPGPSPFPLSGIIATLEEMVTEQEFDAEQ
                     ARFLWRLFCMARGKTTAAAVSSVSEDESEPEITLAELKREVAERRRAEKELRESERRF
                     RDIAEFIPQIIFEMDADCLLTFVNRQAYDIYGYTPRDVKKGVMGLDLVHPSDLPRIRQ
                     TLQAIAEGKPSTGSEFTAVRKDGSTFPVIAHVARIMKGGRLAGWRGIIVDITERKQAE
                     EKLKESERKFRDIAELSPQVIFEQGPDGVVTYANKRGLELFGYTEADLTSGAVMGMDL
                     FPEHEHARVRENLAKMIAGERETTGNEYTARRKDGSTFPAIIYTIAIFSQDRLTGLRG
                     VLVDTTDLKKADRELRERERRFRDLAESVPQVIFEMNETRRITFANRQAFQIFGYDEE
                     DLSRGIDAFSLFVPEQLPAMSENLRRIARGEASTGNEYTALRKDGTSFPVIIHVAPIL
                     DEGRLVGFRGIIVDITERKKAEEELRESRQFLQDIADRIPGVIYQFYAKPGGEMGLSY
                     ISRTAKELFGIETDTSRPFERFLEGLSPDCREALLDSISEAVAKEQDWQFEGDFIKPA
                     GEVMSFAGVSRPSRVGDTLRFNGVLLDITSRKRAEEQLRESERKFRDIAELMPQVIFE
                     IDPKGSVIFVNKQAFEFFGYKEDDLPEGFNCFDHIAPEDRSRAIANLQKAAEDGPSVG
                     NQYAAVKKDGSRFPIIIYVTRIIKDGRLTGYRGIIIDISERTRAEEALKQGEKRLRQI
                     IDLVPHFIFAKDIDGRFILANKAVADNYGTTVEGLIGRMDASFSPSDEEVRHFRNDDL
                     DVMITGRRKYIPGESITDAQGNVRYLETVKIPFTFSGTQTPAVLGVSVDITERMEAER
                     AIARSEQELRTIIAASPIGIGRIRDRIIEWVNEAMCNITGYAIDELTRTSTRILYEKG
                     SEFDRVGALLYRDFQGETKWVRKDGQVLDVLVQLSPMEGGAYIFTTTDITRLKRAEST
                     LKFTQFSVDNAVDSIYWIDNTGSIAYVNNSACLSTGYDRHELLSMKIYEIEPDRPARD
                     WPRYWRNIVEKGSEQFESVYRRKDGTTFPVEVSVSYLPYDEFEYVCAFSRDITERRKA
                     EEALKESEERWQFALEGAGDGLWDWNAQAGTVYFSGQWKEMIGYADKEIGDSIEEWDT
                     RLHPDDRDDVHARLEDHLEGRSSVYISQYRMRCKDGTYKWILDRGKVIRWTGDGRPLR
                     VIGTHTDITEHKRTEERLRLDESRTEALLRIHQMGNRPLEEITAFTLEESIRFTKSAI
                     GYLAFLNDDESVLTMHSWSKKAMEECRIQDKPISYPLETTGMWGDPVRRRRPIITNDY
                     AAPGVTRKGHPEGHVEVRRHLGVPIFDGERIVAVIGVGNKENEYGESDINQLQLMAQG
                     MIRLLKQKAAQEALSVSESRLRAVIDSARDAIFIKDRDRRYIVANGAMSELFGIPMQK
                     ILGATDRELFPDKTADHIETVDGRVLKGETIEEEMARPVAATVYVFHTIKVPLKNDRG
                     EITGLCGIARDITERKHLESQLLQSQKMEAVGTLAGGVAHDFNNLLSAIMGYASLLQM
                     KMDKENPLYGYASQILVSSEKAANLTQSLLAFSRKQVINLKPVVINDTVEKLHRLLER
                     LIPEDVEFRIHRNSERLVVMADPGQLDQVIMNLVTNARDAMPRGGKLTITVDRATVGN
                     EFITTHGYGRAGEYALIAISDTGIGMDSRVLEKIFEPFFTTKGVGRGTGLGLAIVYGI
                     IKQHNGYIDVTSRPGEGSVFCVYLPLVWLEPAREERSAAIIGGTETVLIAEDNADLCQ
                     LSVQVLQDHGYTVLAANDGAMAVETFRRHRGEISLVILDVVMPRMNGKEACEAIRLID
                     PSTRVIFTSGYTDDIIQEKGMLNEEYDFLGKPITPATLLRKIREVLDRK"
     gene            complement(7226..7870)
                     /locus_tag="PKN85_01105"
     CDS             complement(7226..7870)
                     /locus_tag="PKN85_01105"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNT43031.1"
                     /translation="MFELVKMVLDFVIHIDVHLSELIRSYGLWTYLILVLIIFSETGL
                     VVAPLLPGDSLLFAAGAFAARGDFNIFWLFVLLSIAAVLGDTVNYWVGSYVGPKVFHN
                     ENVRFLNRKYLDRTHEFYEKYGGKTIIIARFVPIIRTFAPFVAGIGSMTYGKFISYNV
                     IGGILWIGAFAYAGFFFGNIPMVKHNFTLVIFAIIILSVMPGVIEIIKHKMKKA"
BASE COUNT         1908 a         2368 c         2333 g         1397 t
ORIGIN      
        1 attggccttt gcaggacgga gcacatgttc tttgagggcg accgcatcga cgccgtccgg
       61 gagatgatca tcgccgatga cgaggcggga agaagaaagg cgctggcaaa actgctgccc
      121 taccagaaga aggattttgt cggcctcttc acggtcatga aaggccttcc cgtgaccgta
      181 aggaccctcg acccgccact ccacgaattc ctccctcaca ccgagagaga gacgcgggaa
      241 ctcgccagga agatgggtat ctcctatgaa aaggtcctcg ccaaggtgga aagcctccac
      301 gaggcaaacc ccatgctcgg gcaccgcggc tgccgcctcg gcatcgtctt ccccgagatc
      361 accgagatgc aggccagggc gatcttcgag gccgcctgcg aggtgaagaa gaaaggcatc
      421 aacgtgaagc ccgaggtcat gataccgctc gtcggcaacg tccacgagct caagcttcag
      481 aaggcgatcg tcgaagacgt cgccaacgag atactgaaga agaacaaggt gaagatcgat
      541 tacatgatcg gcaccatgat cgagataccc cgggcggcga tcaccgccga tgagatcgct
      601 caggaggcgc agttcttctc cttcgggacg aacgacctca cccagatgac cctcggcatg
      661 agccgtgacg acgccggcaa gttcctgagc gactacgtca aaaaagagat ctacaccttt
      721 gaccccttcc agaggatcga tgagggcggt gtgggaaggc tcatagagct cggcgtggcc
      781 cttggccgcg gcgtccgcaa gaacctcaag atcgggatct gcggcgagca tggcggcgaa
      841 ccgaattccg tcgagttctg ccacaggacg ggctttgact acgtgagctg ttcgcccttc
      901 cgcgtcacga tagcaaaact ggcagccgcc cgggcagccc tgaaggagaa acaggcaaaa
      961 ccgaaaaaag cggcaaagaa aaaataatgt cctttcggca ggccccgcgg gtcttatcgg
     1021 acccgcgggc ctgttctttt catccccgcg cgccgaccag cgtgtcccgc gtatcccacc
     1081 ccactacttc catgttcttt ttccccttcc atctccttgt tgttgttaaa cattccgcca
     1141 cctggccgat aacacttaca gtcccatacg caggtcccga aggaagccgc gcaaaccggc
     1201 ccttgactgc aggagtaccg ttggagttca atgaaaagat tcttgtcatc tctgcgtgaa
     1261 gatcccgaga agaccgcggc acagtgcgtc cagcgcctgt ggcaggcgac aggagaaagt
     1321 cccgctgaag cgggaaagtg caccgccttg gcccgcctgc ttgtcgacgg gcttgccgac
     1381 agcgtggagg cgatggatcc gggaaggttc atggattcca tcgatgcctg ccgacccgat
     1441 cacgcaacca tgccgggccc atccccgttc cctctttccg gcatcatagc caccctggag
     1501 gagatggtca cggagcagga gttcgatgcg gaacaggccc gattcctctg gaggcttttt
     1561 tgcatggccc gggggaagac caccgccgcg gccgtctcga gcgtctctga ggacgaaagt
     1621 gaacccgaga tcacccttgc ggaactaaaa agagaggtcg ccgagagaag aagggccgag
     1681 aaggagctcc gggaaagcga acgccgcttc cgcgatatcg ccgagtttat tccccagatc
     1741 atcttcgaga tggacgccga ctgcctgttg acctttgtca acaggcaggc ctacgacatc
     1801 tacggatata ccccccgcga cgtcaagaaa ggcgtcatgg gcctcgatct cgtccacccc
     1861 tcagacctcc cgaggatcag acagaccctt caggcgatag ccgagggaaa accctccacg
     1921 ggcagcgagt tcaccgccgt caggaaagac ggcagcacct ttcccgtcat agcccacgtc
     1981 gcgcgcatca tgaaaggcgg gagactcgcc gggtggcggg gcatcatcgt cgacataacc
     2041 gagaggaaac aggccgagga aaagcttaag gagagcgagc ggaagttccg ggacatcgcc
     2101 gagctctctc cgcaggtcat cttcgagcaa ggtccggacg gcgtcgtcac ctacgccaac
     2161 aagcgggggc ttgaactctt cggctacacc gaggcggacc tcacctccgg cgccgtgatg
     2221 gggatggacc ttttccccga acacgaacac gccagagtgc gcgaaaacct cgcgaagatg
     2281 attgccgggg aaagggagac cacgggcaac gaatacacgg cgagaaggaa ggacggcagc
     2341 accttccccg cgatcatcta taccatcgcc atcttcagtc aggacagatt gacgggcctg
     2401 cgcggtgtcc ttgtcgacac cacggatctg aaaaaggccg acagggagct ccgcgaacgg
     2461 gaacgaagat tcagggacct cgcggaatcc gtcccccagg tcatcttcga gatgaacgag
     2521 acgaggagga tcaccttcgc caacaggcag gccttccaga tatttggata cgacgaagaa
     2581 gacctctcgc ggggcatcga cgccttttcc ctctttgtac cggagcagct tcctgccatg
     2641 agtgagaacc tccgaaggat agcacggggg gaggcttcca cgggcaatga gtacaccgcc
     2701 ctgagaaagg acgggacctc attccccgtt ataatccacg tcgcgcctat cctggatgag
     2761 ggccgtctcg ttggtttcag ggggatcatc gtcgacatca ctgaacggaa gaaggccgag
     2821 gaggagcttc gggaaagcag gcagttctta caggacatcg ccgaccgcat ccccggcgtc
     2881 atctaccagt tctacgcgaa acccggcgga gagatgggcc tttcctacat cagccggacc
     2941 gcaaaggagc tctttggcat cgaaaccgac acgtcccgcc ccttcgaacg gttcctcgaa
     3001 gggctcagcc ccgactgccg ggaggcgctc ctcgattcga tcagtgaggc cgtcgcgaag
     3061 gaacaggact ggcagttcga aggcgacttc ataaaacctg cgggagaggt catgtccttc
     3121 gccggcgtgt ctcgtccctc acgggtcggc gacaccctga ggttcaacgg ggtgctcctc
     3181 gatataacat cgcgcaagcg cgcggaagaa cagctcaggg agagcgagcg gaagttccgg
     3241 gacatagcgg agctcatgcc gcaggtgatc ttcgagatcg acccgaaagg ctccgtcatc
     3301 ttcgtgaata aacaggcctt tgagttcttc ggatacaaag aggatgacct tcccgagggg
     3361 ttcaattgct tcgaccacat agctccggaa gaccgctcga gggccattgc gaaccttcaa
     3421 aaggcagccg aagacggccc ttcggtcggc aatcagtacg cggccgtaaa gaaggacggg
     3481 agccgattcc ccatcatcat ctacgtcacg aggatcataa aggacggcag gctcacgggc
     3541 taccggggga tcatcataga catatcggag cgcacccgcg ccgaggaggc cctcaagcag
     3601 ggtgaaaagc gcctccggca gatcatcgac ctcgtgcccc atttcatctt cgccaaggac
     3661 atcgatggaa ggttcatcct tgccaacaag gccgtcgctg acaactacgg caccacggtg
     3721 gaaggtctga tcggcaggat ggatgccagc ttctcgccat ccgatgaaga ggtgaggcat
     3781 ttcaggaacg acgacctcga tgtgatgatc acaggcagga ggaaatatat ccccggcgag
     3841 tccatcaccg acgcgcaggg gaatgtgcgc tatctcgaga ccgtcaagat ccctttcaca
     3901 ttctcgggaa ctcaaacccc cgccgtcctg ggcgtgtccg tggacatcac ggaacgcatg
     3961 gaggctgagc gcgccattgc ccggtccgag caggaactca ggaccatcat cgccgcctcg
     4021 cccatcggca tcggccggat ccgcgacagg atcatagagt gggtgaacga agcgatgtgc
     4081 aatatcaccg ggtacgcgat agacgaactc acccggacga gcactcgcat actctatgag
     4141 aagggatcgg aattcgaccg ggtcggagcg ttgctctatc gggacttcca gggagagaca
     4201 aaatgggtga ggaaggacgg gcaggtgctc gacgtgcttg tccaattgtc gccgatggaa
     4261 ggtggggcct acatcttcac cacaaccgac atcacccggc tgaaacgggc cgaaagcacg
     4321 ctgaaattca cccagttctc cgtcgacaac gccgtggaca gcatctactg gatcgacaac
     4381 accgggagca tcgcctacgt gaacaacagt gcgtgcctct ctacagggta cgaccgccac
     4441 gagcttctct ccatgaagat ctacgagatc gaaccggacc gtccggctcg ggattggccc
     4501 cggtactggc ggaacatcgt cgaaaaaggc tccgagcagt ttgaatccgt gtaccgcagg
     4561 aaggacggga ccacgttccc cgtcgaggta agcgtcagct accttccata cgatgagttc
     4621 gaatacgtct gcgccttttc ccgggacatc acggagcgca ggaaggccga ggaggccctg
     4681 aaagagagtg aagagcgatg gcagttcgcc ctcgaaggcg ccggtgacgg tctgtgggac
     4741 tggaacgcgc aggccggcac ggtctatttt tccgggcaat ggaaggagat gatcggctat
     4801 gcggacaagg agatcggcga ttccatcgag gagtgggaca cacgcctgca ccctgacgac
     4861 agggatgatg tccatgcccg gctcgaagac cacctggaag gcaggtcatc cgtctacatc
     4921 agccagtacc ggatgcggtg caaggacggc acctacaaat ggatactcga caggggcaag
     4981 gtcatacggt ggaccgggga cggcaggccg cttcgcgtca tagggaccca cacggacata
     5041 acggaacaca agaggaccga ggagcggctg cgcctcgacg aatcgcggac ggaggccctc
     5101 ctcaggatcc accagatggg caaccggccc ctggaggaga taaccgcctt cacactcgag
     5161 gaatcgatcc ggttcacgaa gagcgccata ggctatctcg cgttcctcaa cgacgacgaa
     5221 tccgttctca ccatgcattc ctggtcgaag aaagccatgg aggaatgccg catccaggac
     5281 aaacccattt cctatcccct tgagacaacc ggcatgtggg gagacccggt gcgtagacgc
     5341 aggccgatca tcaccaacga ttacgccgcc ccgggcgtta cccgcaaagg ccatcccgag
     5401 ggccatgtgg aggtgaggcg acacctgggc gtccccatct tcgacgggga gaggatcgtc
     5461 gccgtcatcg gtgtgggcaa caaagagaac gaatacgggg aatcggatat caaccagtta
     5521 cagctcatgg cgcagggaat gatccggctc ttgaaacaga aggcggccca ggaggccctg
     5581 agcgtatccg agtcccgcct gcgggcggtc atcgacagcg cccgggacgc cattttcatc
     5641 aaagacaggg accgccgcta catcgtcgcc aatggggcca tgtcggaact cttcggcatt
     5701 cccatgcaaa agatactggg cgctaccgac cgggaattgt tccccgacaa aacggccgac
     5761 cacatcgaaa cggtcgatgg ccgcgtgctt aagggcgaga cgatcgagga agaaatggca
     5821 cgtcccgtgg ccgccacagt ctatgtcttt cacaccatca aggtccctct gaagaacgac
     5881 cgcggcgaaa taacgggcct gtgcggcatt gcccgggata tcacggaacg caagcacctc
     5941 gagtcccaac tcctgcaatc gcagaagatg gaggccgtgg ggacacttgc gggaggggtc
     6001 gcgcatgatt tcaacaacct cctgagcgcc atcatgggat acgcaagtct cctgcaaatg
     6061 aagatggaca aggaaaatcc tctttacggc tacgcctccc agatactcgt ctcttccgaa
     6121 aaggcggcca atctcaccca aagcctcctt gccttctcaa ggaaacaggt catcaacctc
     6181 aagcccgtgg tcataaacga taccgtggaa aagctgcacc gccttctcga gcggctcata
     6241 ccggaagacg tggaattcag gatacacagg aattcagaac gcctcgtcgt catggcggac
     6301 cctggtcaac tcgaccaggt gatcatgaac ctcgtcacga acgcccgcga cgccatgccc
     6361 cggggaggga agctgacgat caccgtcgac agggccaccg tgggcaatga gttcatcaca
     6421 acgcacgggt acggcagagc gggggagtac gccctcatag ccatctcgga tacaggcatc
     6481 ggcatggata gcagggtgtt ggaaaagatc ttcgaaccct ttttcaccac caagggggtc
     6541 ggacggggca ccggcctcgg actcgccatc gtctacggca tcatcaagca gcacaatggc
     6601 tacatagacg tcacgagccg tcccggtgag ggaagcgtct tctgcgtgta cctccccctt
     6661 gtgtggctgg aaccggcgcg tgaggaaagg tcggccgcca tcatcggcgg cacggaaacc
     6721 gtcctcatcg ccgaggacaa cgccgacctc tgccaactct ccgtccaggt ccttcaggac
     6781 cacggataca cggtcctcgc tgccaacgac ggcgcaatgg ccgtcgaaac cttccgaaga
     6841 cacaggggcg agatctccct cgtcatcctc gacgtcgtca tgcccaggat gaacggcaag
     6901 gaggcctgcg aggccatacg cctcatcgac ccgtccacca gggtcatctt cacgagcggg
     6961 tacacggacg acataatcca ggaaaaaggc atgctcaacg aagaatacga cttcctcggc
     7021 aaacccataa ccccggcaac cctcctcaga aagataaggg aagtgctgga caggaagtaa
     7081 agacggttcc agagttcaag agttcaagag taaaaagcaa aagatgagga gatggtccgt
     7141 tgcgctgcgt ccgggaaaac ggagatcccg taccgcgctg tccgccctct ttctttgtct
     7201 ttgcccttga actctggaac catctttacg cctttttcat cttgtgcttg atgatctcga
     7261 tgacgccggg catgacggag aggatgatga tggcgaagat gacgagggtg aagttgtgct
     7321 tcaccatggg aatattgccg aagaagaagc cggcgtaggc gaaggcccct atccagagga
     7381 tgccgccgat gacgttgtag cttatgaatt tgccataggt catgctgccg atgccggcga
     7441 cgaagggtgc gaaggtccgg atgatgggca cgaaacgggc tattatgatc gtcttgccgc
     7501 cgtacttctc gtagaactcg tgggtcctgt ccaggtactt ccggttcagg aaacgcacgt
     7561 tctcgttatg aaagaccttt ggacccacgt agctccctac ccagtaattg acggtatcgc
     7621 ccagcacggc cgctatcgag agaaggacaa aaagccagaa tatgttgaaa tcaccgcggg
     7681 cggcgaaggc cccggcggca aagagaaggg agtctccggg aagcagcggc gcgaccacaa
     7741 ggcccgtctc agagaatatg atgagaacga ggatgagata cgtccaaagc ccgtagctcc
     7801 gtatgagttc gctcaggtgg acgtctatgt ggatgacgaa atcgagcacc atcttgacga
     7861 gttcgaacac gctatacatc cctcctgagt cggtttagcc gggaaaattc cctgtatact
     7921 acacttttgc cccctcttga gtcaatgaat tggtgcagac gccatcttct tgtgattgcc
     7981 cgaacgctgt cttatactgt ttacac
//
LOCUS       DAOQTR010000023         7996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_9984_length_7996_cov_3.921295, whole genome shotgun sequence.
ACCESSION   DAOQTR010000023 DAOQTR010000000
VERSION     DAOQTR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9984_length_7996_cov_3.921295"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..2614)
                     /locus_tag="PKN85_01110"
     CDS             complement(41..2614)
                     /locus_tag="PKN85_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43032.1"
                     /translation="MKLSGFFPKRFGSRLFIMTIIAGFIPIAIFSILIHTFSGRFESS
                     VQRAVEDGYREEWAQSSTLLTKIGEASIRQKAYDIAAQLNLTLQSHPYMTLGDLRRDA
                     KFRSVAVQGIGDRGYTGLHETDTGIIRFHRDHEVENTGTRAFEKTLPAYWRIIEAGRG
                     GKPSGGYFNWTEPDGSTTQKYMYVVPLSQRTADGKRLSVFVTTYLDEFTKPLREAQAI
                     QQKTARNLISTTSYLLGSFRDNGLLFMGIGILLVSFVALAIGMYFSRGISRLSEATRR
                     VNEGDFKVSVEPVFSGEIKTLMEDFNRMVARLDETTVSKGLLEESEKRLLEANIDLRR
                     EIAVRSLAEKALAAEKERLAVTLRSIGDGVITVDTEGKVILINKAAETLTGYSQQEAD
                     GRSFTEIFPLRDNGIDGADEDPVRALLKGTEPASLSTPAVLVGRDGTERLVATTASLI
                     RSEDATVIGAVIVFRDVTEQHRMEAELLKARKLESIGTLAGGIAHDFNNLLAVILGNI
                     SFARMLLDDDQKAVKRLDEAEKATIRGKDLSYRLLTFARGGAPVKKLTVLDDVIRDAA
                     ELTVSGSTSRCVYNFPEDLYRAQIDEGQIRQVVHNLVMNARESMPDGGTITIAAENVD
                     LVHPEGTLTPGRYIRLTFQDKGCGISPEDLERVFDPYFTTKEMGSEKGMGLGLAICYS
                     IVRSHNGYISISSEPGAGTTVSVYVEAYEGDAESWPAPGVMRQGKGKKVLYMDDEEQV
                     RDLAGQILEHLGYNVEFARDGAEAIELFRNGVASGAPYDLVVLDLTVPGGMGGREAME
                     NILAINPMVKAVVSSGYVNDAVVRDYKKYGFSGVVAKPYSIEQFRRVIESVFNGKPVA
                     S"
     gene            complement(2713..3039)
                     /locus_tag="PKN85_01115"
     CDS             complement(2713..3039)
                     /locus_tag="PKN85_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43033.1"
                     /translation="MELFVVMDRSILGRGVFAVFSSIEKAQSFSDDMYRNTRFQCEVK
                     TCPLIGGSDVPGRVYAAHFYDDFYDTHVFDGIYSESNLAYDAVGGKGLIIRFVIDSPE
                     DKEIIA"
     gene            3199..3936
                     /locus_tag="PKN85_01120"
     CDS             3199..3936
                     /locus_tag="PKN85_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT43034.1"
                     /translation="MSLKDRTALITGAGQGIGKACAEVFGERGARLILLDKNRKTLPH
                     VARDLAAKGVDVVFRIIDLTRTQPLVKTVEELLADRRIDILVNNAGFDRPGITAKIDK
                     TDYNAVLSIHLGVPFLLSKLLLPQMRANKWGRIVNVSSIYGLMGAKGEVAYAAAKAGV
                     LGLTKTLAREGGRDGVTINAVVPGMIRTPPIMRMPDKYRDPIIVESMLGRIGEPEEVA
                     RVVAFLASNDASYITGTEIKVSGGWGV"
     gene            complement(4058..5809)
                     /locus_tag="PKN85_01125"
     CDS             complement(4058..5809)
                     /locus_tag="PKN85_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT43035.1"
                     /translation="MARVISFKTGRRTPTLFRLLRDRYLKDGIDDGYIAFKWEEWGKN
                     PSITVFRAVKNNRVVGWIFYDRKTSTIEEMLVEGTWKGKDPRPEMLDTLIARESLVAA
                     SLLTADQEKYELLIGYGFRPTLFFHRNGFDLVRMELSTSVLLKKTAAGKPFRGYRKRE
                     RVAVEKIPPTQTYEEIKKGLAGLIDRLGGLRRFVKPGQTVAIKPNVVSDHGLKDGKVV
                     GGIVTDIRVVKALTEMLLGLASHVYITEGASINRSATSKMFSHYGYDEIVALDPAKIS
                     LVDLNTDRLIDKPVPGCKRMSSRKIPATLEMVDVIINVPVLKIHFAAISSLAIKSLQG
                     AVPPIEKYMSHFFGLWQSLVNIHHLVKPKLTIIDGLTGLEDFGPVSGTPIKMDVLIGG
                     TNAVAVDAVAMEIMGIDPRTSPPVFLAWMQGLGPLEKNKINVVGTPIEEVAKKFVQPA
                     IDVSGGASLKILADEACPGCKGYLHFVLSKLRRPDPADPTRSLIDRPLEKKANIFLGP
                     STTVPVNPDETNIFMGICQQHHAGLGTHMPGCPPHAEVITKAVYSLFPDIEPPKYADE
                     TEETKLGKMLEEILQNR"
     gene            complement(5921..6124)
                     /locus_tag="PKN85_01130"
     CDS             complement(5921..6124)
                     /locus_tag="PKN85_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43036.1"
                     /translation="MIDPVSSIMSYAMSRNEATMSLAKSRLEAEAAVAQVILEAAKNI
                     QRMTEVSSSSASPFLGSKIDVYV"
     gene            6366..6866
                     /locus_tag="PKN85_01135"
     CDS             6366..6866
                     /locus_tag="PKN85_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT43037.1"
                     /translation="MGLAEELGLERPIETVEHESLLNIIYTGTMIARAAFRYFQGHRL
                     TDAQFNVLVQLKYSDGKALSQAELGRRLVVNKADMTGIVDRLERTGLVERSAHPNDRR
                     VNLVRMTAEGEKAVNVLEPGYLKGVGLLMSGISRSDLMRLNRTLEKVRSNAKGKLVPN
                     GGREGR"
     gene            6863..>7996
                     /locus_tag="PKN85_01140"
     CDS             6863..>7996
                     /locus_tag="PKN85_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNT43038.1"
                     /translation="MRNQRSLFRALFEEGARAIFKTYLKVFHRIKVGGIDRLPRTFDK
                     LMVISNHASLLDGIILWTYIDVDLRILVNRGRARELLLRPFMQNRYTVQIDTMNPYSL
                     KGVIEKVNRGTALLVFPEGRITRTGNLMKIYEGAGFVAYKTGAKILPVHLGGTYDTIF
                     AKKHPGRKIFAPITLTIGEIRPPIELEHFQPHDRKREAARAIYGMLCEMFYEARYRPS
                     TLGREFIHICRKRGPNTLFKDATGVEVSCRKALVGGLLLGEKLSRHEGANIGVLLPNL
                     VATALVIYGVLMFRKVPVLLNYSGGPRALRHSMELAGLKVIVTSRVFLERVKIDPGVF
                     EDARVIYLEDLKASIGAGDKLRAILRSVFPGGLARVSDGDHKGP"
BASE COUNT         1624 a         2235 c         2380 g         1757 t
ORIGIN      
        1 ataaggtcac gatctgatga ccttcctcgg taacatttaa ttatgaggca actggctttc
       61 cgttgaacac gctttcaatg acccttctga attgctctat ggagtaaggt tttgccacga
      121 ccccggaaaa gccgtatttc ttgtagtcgc gtacgacggc gtcattcacg tatccgctgg
      181 acacgacggc cttcaccatg gggttgatgg cgaggatgtt ctccatcgcc tcgcggcccc
      241 ccatgccccc gggcacggta aggtcgagca ccacaaggtc gtagggggca cccgaggcaa
      301 ccccgtttct gaagagttct atcgcctcgg ccccgtcccg ggcaaactcg acgttatatc
      361 ccaggtgttc cagtatctgt cccgccaggt cccggacctg ttcctcgtca tccatgtaga
      421 ggaccttctt tccttttccc tggcgcatga caccgggggc cggccatgat tccgcgtccc
      481 cttcataggc ctcgacatag acggacaccg ttgttccggc tcctggttcg gaagagatgg
      541 atatatagcc gttatggctc ctgactatgg aatagcagat cgccagacct aatcccattc
      601 ccttctcgct gcccatttcc ttggtcgtga agtaagggtc gaagacacgt tcaagatcct
      661 cgggggagat gccgcatccc ttgtcctgaa aggtcagacg gatatatctg ccgggagtca
      721 gggtcccttc gggatgaacg aggtccacgt tctcggcggc tatcgtgatc gtgcccccgt
      781 cgggcatgga ttcccgggcg ttcatgacca gattgtggac gacctgcctg atctgtcctt
      841 cgtcgatctg tgccctgtac agatcttcgg gaaagttgta gacgcatctt gaagtggagc
      901 cgctcacggt aagttcggcg gcatcgcgta taacgtcgtc gagaacggtg agcttcttca
      961 ccggtgcgcc gccccgggcg aaggtgagga gccggtagga aaggtcctta ccccggatcg
     1021 ttgctttttc cgcctcatcc agcctcttca ctgccttctg atcgtcgtcg aggagcatcc
     1081 gcgcgaagga tatgtttccc aggatgactg cgaggaggtt gttgaaatcg tgggcgatgc
     1141 cccctgccag ggtgccgatg gactcaagct ttcgcgcctt gaggagttcg gcttccatcc
     1201 tgtgctgctc ggtaacatcg cggaaaacta tcacggcgcc gatgaccgtg gcatcttcgc
     1261 tgcggatgag cgaggccgtt gtggccacga gccgttccgt cccatcccgt ccgacaagaa
     1321 cggcgggtgt cgagagagag gctggctcgg tgcccttgag gagggctctg actgggtcct
     1381 cgtctgcccc gtcgatgcca ttgtccctca gggggaagat ctccgtgaag gacctgccgt
     1441 cggcttcctg ctgcgagtag ccggtgagcg tttcagcggc cttgttgatg agtatgacct
     1501 tgccttcggt atcgacggtt atgacgccgt ccccgatgga cctgagcgtg acggcaaggc
     1561 gctccttctc cgcggccagg gccttttccg ccagggagcg gacggcaatc tcccgcctga
     1621 ggtcgatgtt tgcctcaagg agtctttttt cgctctcttc gaggagcccc ttggagaccg
     1681 tcgtttcgtc gagacgagcc accatgcggt tgaaatcctc catcagcgtc ttgatctctc
     1741 ccgagaaaac gggctcgacg gacaccttga agtcaccttc attgaccctc ctggtcgctt
     1801 cgctcaggcg cgagatgcca cgcgagaaat acatcccgat ggcgagggcg acgaaagaga
     1861 caaggaggat ccctattccc atgaatagga gtccgttgtc acgaaaagac ccgaggagat
     1921 acgatgtggt gctgataagg tttctcgccg tcttttgctg tatcgcctgg gcttccctca
     1981 agggtttcgt gaactcgtcc agataggtgg tcacgaacac gctgagacgt ttgccgtcag
     2041 cggtgcgctg ggacaggggc acgacataca tgtatttctg ggtggtggaa ccgtcaggtt
     2101 ctgtccagtt gaaatacccg cccgacggtt tgccgccgcg ccccgcctct atgatccgcc
     2161 agtatgcggg aagcgttttc tcaaaggccc gtgtgccggt gttttcgact tcgtgatcac
     2221 ggtgaaagcg gatgatgccg gtatccgttt cgtgaagacc cgtgtaacca cggtccccaa
     2281 tgccctggac ggccacgctg cggaacttgg cgtcgcgtct gagatcgccg agtgtcatat
     2341 agggatggga ctggagggtg aggttgagct gggccgctat gtcgtaagcc ttctgacgta
     2401 tcgatgcctc accgatcttt gtgaggaggg tggaactctg ggcccactcc tctctgtacc
     2461 cgtcctccac ggcgcgctgg acgctcgatt cgaaccgtcc tgagaaggtg tggatgagga
     2521 tgctgaagat cgcgataggg atgaaccctg ctatgatcgt cattatgaaa agacgggaac
     2581 cgaaacgttt cggaaagaac ccggacagct tcatcgacta gcagtctccc tctgcattaa
     2641 gagtaacata aatggtatcc tcaataaagt cgatgataat tgtcacatgg gcgccagggc
     2701 ggtaacctgt gttcaggcga tgatttcctt atcttcgggg gagtcgatga cgaaccgtat
     2761 gatgaggcct ttcccgccga cagcgtcata ggcgaggttg ctctcggagt atatgccgtc
     2821 gaagacgtgg gtgtcataga aatcgtcgta gaaatgagcc gcgtagaccc tccccgggac
     2881 atccgaccct ccgattaggg ggcaggtctt cacctcacac tgaaaccggg tgtttcggta
     2941 catgtcatcg ctgaacgact gcgccttttc gatgctggaa aagacggcga atacgccgcg
     3001 gcccaatatc gatctgtcca ttaccacgaa taattccatg tatcccatct tcctttccgg
     3061 atagatccgt actataggag ataaaggaac tcggcgaaga aatcaagtct tttgacattt
     3121 tgttgtaacc cgttcccgcg ttgcggtaga ataacgacgt tccagcggaa cacttctgaa
     3181 ccatcacgag gagccgtcat gagcctgaaa gacagaactg cattgatcac aggggccgga
     3241 cagggtatcg gcaaggcctg tgccgaggtt tttggggagc ggggggcacg tctcattctc
     3301 ctcgacaaga acaggaagac gcttcctcac gtcgcgcggg acctggcggc gaaaggcgtc
     3361 gacgttgtct tccggatcat cgacctcacc cgcacacagc ctctcgtgaa gacggtcgag
     3421 gagcttctgg cagaccggag gatagacatt ctggtcaaca atgccggttt tgacaggccg
     3481 ggcatcacgg cgaagatcga caagaccgac tacaatgccg ttctgtcgat ccatctcggt
     3541 gttccctttc tcctctccaa gctgctcctt ccgcagatgc gcgccaacaa gtgggggcgc
     3601 atcgtcaacg tgagctccat atacgggctc atgggggcca agggcgaagt ggcctacgcg
     3661 gcagccaagg ccggggtcct gggcctgacc aagaccctgg cgcgggaagg gggaagagac
     3721 ggtgtgacca taaatgccgt cgtcccgggc atgatacgga ccccgcccat catgcggatg
     3781 cccgacaagt acagagaccc catcatagtg gagagcatgc tcggcaggat aggtgaaccg
     3841 gaagaagtgg cccgcgtggt cgcctttctt gcctccaacg acgcatcata cataaccggc
     3901 acggaaataa aggtgtcggg agggtgggga gtctagaacc ggcttgaata gcttaagggg
     3961 cttgaatcgc ttgagagttt gagaaacatt caagaaacct gtgcggttcg ggtctttggt
     4021 ttctaaccct caagcgattc aagccttcaa accgtcttta ccggttctgc agtatttcct
     4081 ccagcatttt ccccagtttt gtttcttccg tttcgtcggc atacttgggg ggctctatgt
     4141 cgggaaaaag gctgtagacg gccttggtga tgacctccgc gtggggaggg cagccgggca
     4201 tgtgggtgcc gagaccggcg tggtgctgct ggcagatacc catgaagatg ttcgtctcgt
     4261 cggggttgac gggaacggtt gtcgatgggc ccaggaagat gttggccttc ttttcgaggg
     4321 gcctgtcgat gagagaccgg gtggggtcag cgggatcggg cctgcgcagc ttggaaagga
     4381 cgaagtgcag ataacccttg catcccgggc acgcctcgtc ggcaaggatc ttgagcgagg
     4441 cgccgccgct gacatcgatg gcgggctgca cgaatttctt cgccacctct tcaatggggg
     4501 tgccgacgac gttgatcttg ttcttctcga gcggtcccag gccctgcatc caggcgagga
     4561 ataccggggg agatgtcctc gggtcgatac ccatgatctc catggctacg gcatcgacgg
     4621 ccacggcgtt cgttccgcct atcaggacat ccatctttat cggggtgccc gagacgggcc
     4681 cgaagtcctc gaggcctgtg aggccgtcga tgatggtcag tttcggcttg acgaggtggt
     4741 ggatgttcac gaggctctgc cagagaccga aaaagtggga catgtatttc tctatgggcg
     4801 gtacggcacc ctgaagactc ttgatggcca gcgaggaaat ggcggcgaaa tggatcttca
     4861 gcaccggcac attgatgatg acgtcgacca tttcaagggt agcggggatc tttcgggatg
     4921 acatgcgttt gcagcccgga accggcttat cgatgagcct gtccgtgttg aggtcgacga
     4981 gactgatctt cgcggggtcg agggcgacga tctcgtcata tccataatgg ctgaacatct
     5041 tcgacgtcgc gctgcggttg atcgaagcgc cttcggtgat gtacacgtgg gatgcgagac
     5101 cgaggagcat ttccgtcagg gctttgacga cacggatgtc ggtgacgatg ccgcccacga
     5161 cctttccgtc cttaaggcca tggtcgctca cgacgttggg cttgatcgcg acggtctgcc
     5221 cgggtttgac gaaccgcctg agcccgccga gcctgtcgat gagaccggca agtcctttct
     5281 tgatctcttc gtatgtctgg gtgggtggta tcttctccac ggcgacgcgt tccctctttc
     5341 gataaccgcg gaatggtttg cccgcggccg tcttcttcag cagcaccgac gtgctcagtt
     5401 ccatcctgac caggtcgaaa ccgttgcggt ggaagaaaag cgtgggcctg aaaccatacc
     5461 cgataagaag ctcgtacttc tcctggtccg ccgtgagaag agatgccgct acgaggcttt
     5521 cgcgggcgat gagcgtgtcg agcatctccg ggcgggggtc cttgcctttc cacgttccct
     5581 ccacgagcat ctcctcgatg gtgctcgtct ttctgtcgta gaagatccat cccacgaccc
     5641 tgttgttctt cacggcgcgg aagaccgtga tggaagggtt ctttccccac tcttcccatt
     5701 tgaaggcgat ataaccgtcg tcgatgccgt ccttcagata acggtctctg aggagcctga
     5761 aaagggtggg ggttcgcctt cccgttttga aagatattac tcttgccatg ctgttgacct
     5821 cctcctccgt gcgagtgtgg atcctacccc gcccccgtca cacgtgccaa gattatatca
     5881 gtttccgggc tagcgggaaa gaggttaggc gttccgcgct ctaaacgtag acatcgatct
     5941 ttgatccgag aaaaggcgag gcggaggacg aggacacctc ggtcatgcgc tggatgttct
     6001 ttgcggcctc cagtatgacc tgcgcaaccg ccgcctccgc ctccaaccgg ctcttcgcca
     6061 gagacatggt ggcctcgttg cggctcatgg cgtagctcat gatggaagaa acagggtcga
     6121 tcataccatc ctctggagac tttatcgggg gatagggagc aaacttaagg ggataataaa
     6181 agaaggttca agggttcaag agtaaaagaa aaaagcaccg gataccttcg gccggtgggc
     6241 gaattgcgtt gacagccgat cccccttcgc ggtctttccc ttgaaccctt gttgaccttg
     6301 acatctgccc gaagccatag tatacattca aacagttcat atctaaacta taaggaggtt
     6361 ccttcatggg gcttgcggag gagttgggac tcgaacggcc catagagacc gttgaacatg
     6421 agagtctgct gaatatcatc tataccggga ccatgatcgc cagggccgcc ttcaggtatt
     6481 tccagggaca caggctcacc gacgcccagt tcaatgtgct tgtccagctc aagtacagcg
     6541 atgggaaggc cctctctcag gcggagcttg gaaggcgcct tgtggtcaac aaggcggata
     6601 tgacggggat cgtcgacagg ttggaaagaa cgggtctcgt ggagcgctcg gcccacccca
     6661 atgaccgccg cgtgaacctc gtgagaatga cggcggaagg cgagaaggcc gtcaatgtcc
     6721 tggagccggg ctacctcaag ggggtcgggc tcctgatgtc ggggatatcc cggtcggatc
     6781 tgatgcgcct caacaggacc ctggagaagg tccggagcaa cgcgaaaggc aaactggtac
     6841 cgaacggggg ccgggaggga cgatgaggaa tcaacgtagt cttttcagag cgctctttga
     6901 agaaggagcg cgggccatat tcaagacata tctgaaggtg tttcacagga tcaaggtagg
     6961 ggggattgac aggcttcccc gcactttcga caagctcatg gtcatatcga accacgcaag
     7021 cctcctcgac ggcatcatcc tgtggaccta catcgacgtg gacctcagga tccttgtcaa
     7081 tcggggcagg gcccgggagc tgctgctccg gccctttatg cagaaccggt acaccgtgca
     7141 gatcgatacc atgaacccct attccctgaa aggcgtcatc gaaaaagtga accggggcac
     7201 agcgcttctt gttttccccg agggacgcat cacgaggaca ggcaacctta tgaagatcta
     7261 cgaaggcgcc ggctttgtcg cgtacaagac gggcgcgaag atcctgcccg tgcatctcgg
     7321 cggtacctac gataccatct ttgcgaagaa gcacccggga aggaagatat tcgccccgat
     7381 aacactgacc ataggtgaga tccgtcctcc catcgaactc gaacacttcc agccccacga
     7441 caggaaacgg gaggctgcac gggccattta cgggatgctc tgcgagatgt tctacgaggc
     7501 gcgctacagg ccttcgacgc ttggccgcga gttcatccat atatgcagga agagaggccc
     7561 gaatacgctc ttcaaggatg cgacgggggt cgaggtcagc tgccgcaagg cccttgtcgg
     7621 ggggctcctt ctgggggaga agctctcccg ccacgaaggt gccaacattg gagtgctcct
     7681 gccgaacctc gtcgccacag cgcttgtcat ctacggggtt ctgatgttcc ggaaggttcc
     7741 cgtcctcctc aactattccg gcggaccccg ggccctgcgg cactccatgg aactcgcagg
     7801 cctcaaggtc atcgtcacgt cccgggtgtt cctcgagcgc gtgaagatcg atcccggcgt
     7861 gttcgaggac gcccgcgtca tctatcttga ggacctgaag gcctccatcg gggcaggcga
     7921 caagctgaga gcgatcttaa ggtccgtctt ccccggaggg cttgcccgcg tatctgacgg
     7981 ggaccacaag ggccct
//
LOCUS       DAOQTR010000024         7973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_10014_length_7973_cov_3.548750, whole genome shotgun sequence.
ACCESSION   DAOQTR010000024 DAOQTR010000000
VERSION     DAOQTR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10014_length_7973_cov_3.548750"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..130)
                     /locus_tag="PKN85_01145"
     tRNA            complement(56..130)
                     /locus_tag="PKN85_01145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Val,seq:tac)
     gene            complement(174..1427)
                     /gene="clpX"
                     /locus_tag="PKN85_01150"
     CDS             complement(174..1427)
                     /gene="clpX"
                     /locus_tag="PKN85_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNT43039.1"
                     /translation="MIKKTNGRTIKLNCSFCGKSQDEVKKLIAGPMVYICDECIELCN
                     EIIQEEAKKERFDYIKTAIPKPHEINKLLDDFVIGQDHAKKVLSVAVYNHYKRIESKT
                     QFDDVEIQKSNILLIGPTGSGKTLLAQTLAKILHVPFTIADATTLTEAGYVGEDVENI
                     ILYLLQAADYNVERAERGIVYIDEIDKISRKTDSPSITRDVSGEGVQQALLKIIEGTV
                     ANVPPKGGRKHPQQDYIKVDTSNILFICGGAFHSIEGLIAGRIGKKSIGFGVDVEGGR
                     DKMYSELIRDIQPEDLLKYGLIPEFIGRLPITATLEELSEENLVEILKRPRNAIVKQY
                     KKLFDLVNVKLTFTEGSLRGIAREAMKKKTGARGLRSIMEKVMLDVMYEIPSLSNVKE
                     CVISEEVIVNHERPILIYEEEAESA"
     gene            complement(1431..2015)
                     /gene="clpP"
                     /locus_tag="PKN85_01155"
     CDS             complement(1431..2015)
                     /gene="clpP"
                     /locus_tag="PKN85_01155"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNT43040.1"
                     /translation="MSLVPIVIEKDGRGERAYDIYSRLLKERIVFLGTGIDDMVANII
                     IAQLLFLESEDPSKDIYLYINSPGGIITSGLAIYDTMQYIKAPVVTTCIGQAASMGAV
                     LLAGGEKGKRFALPHSRILIHQPLGGAQGQASDIDIQAKEILRMRESINNILVRHTGQ
                     PLDKVAKDTERDYFMDAQQAIEYGIVDQILEKRL"
     gene            complement(2012..3298)
                     /gene="tig"
                     /locus_tag="PKN85_01160"
     CDS             complement(2012..3298)
                     /gene="tig"
                     /locus_tag="PKN85_01160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNT43041.1"
                     /translation="MKVQVEALDSVRKKVEVLLPRETVSGIRESIYDEIRRSAKIKGF
                     RQGKVPRPIITQYYKEYIEEETRKRMLQNTMFDALKEANVEPITEPVADFLEGDEPGY
                     TLDCEVLPEIELPEYKGIEIEAEPINISDADVEQRIESMRQFHASFEAKQGEATAQKG
                     DFVVIKYQGYRDGEALKQIAADGYPLELGNAMLMPEFENAVIGMKEGEERDVDVTFPD
                     DYPDKDIASKKILFKVTMKEIKQKVLPEVNDEFARDMSFENLEVMRKGVREELIKEKE
                     GFRKSDLTRKALEVLLRDREIPVPKRFLEKRVHGMIEEAQSRYQTDRLSAEELQSISE
                     RMHADFGKRAEDRIKAEIVLARIAEKEDIKADDKDVEERLKTIAEDANKTYNEVKGIY
                     EQYGLVAGLRDNIVEEKAIDFVLANAVVKDAPKENE"
     gene            complement(3531..4316)
                     /locus_tag="PKN85_01165"
     CDS             complement(3531..4316)
                     /locus_tag="PKN85_01165"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNT43042.1"
                     /translation="MIRYSDNEMMALAAGRFIKDGDIVFAGTGVSILAATAAKRIFAP
                     KAVIFFETGGIDPSLDEIPMAVADLRVMAGTSLNSGLIEAFSIVGHRKLHTVAFLGAA
                     QIDRYGNLNTTCIGDYFRPKTRFSGSGGACDVATFASGVITFMQHEKRRFVEKLDYLT
                     SVGWYEGGDSRKELGLRRGGALAVVTNLGVLRFHEETKEMYLAEYFPGVTIEKIVEST
                     GFPIDTSMAVEAETPTPGELQILREEVDPQRLICQAVLETPQK"
     gene            complement(4329..5306)
                     /locus_tag="PKN85_01170"
     CDS             complement(4329..5306)
                     /locus_tag="PKN85_01170"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNT43043.1"
                     /translation="MNRYPEIADRLMGLDEAVSRFVKNGCQLAIGGFTITRNPMAVAY
                     EIVRQRVKDIHLVCHSHGQALDVLVGAGCVGRLEIAYGGNGRYAPTCVRFKKAIQRSE
                     IQFEDYSNYQMSLRFLAGALSIPFIPTKSGLGSDLMNYEGFPAEVRKEEKVASRKYAK
                     MANPFSEDWDEVVLLPALTPDVTILHAQYVGDDGTVRIKGLTFADIEQAKAADRVIIT
                     CEDIVPRSFIRTDPDQNSLPPFFVDAIVKVPYGAHPTACYGFYDYDPKHLNLYRKMAE
                     DDTLFTEYLDEWVYGVGSWEDYLTKVGMEMILKIKANPVIGYAPGLDRK"
     gene            complement(5490..5990)
                     /locus_tag="PKN85_01175"
     CDS             complement(5490..5990)
                     /locus_tag="PKN85_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT43044.1"
                     /translation="MKSTAPNDSPRTTAARKSDKETAMRMQALEETNEALKTLLARGE
                     DDKKLVEDRIKSNVKELVLPYVDKLKLTGLTVEQQTYLEIIETTIKNVFSSFLQKITS
                     KQYHFTPKEIQVATLIREGKTTKQIADIMKVTRSAIGLHRHHIRNKLGLGKAKVNLRS
                     YLLSLQ"
     gene            6330..7088
                     /locus_tag="PKN85_01180"
     CDS             6330..7088
                     /locus_tag="PKN85_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT43045.1"
                     /translation="MNIREITARSALTRTGIEGYDYCLNPYVGCGHGCRYCYATFMKR
                     FTGHSEPWGDFVDVKANVAHVLRRQLKRVREGTLLIGTVTDPYQPLEKRYRLTRDCLS
                     ALVPTPLEVHILTRSPLVVRDTDILRQLSHVEVGLSITTNREDVKQVFEPRAPSIASR
                     VVALKALHDAGLRTYVFVGPLLPMDPGVLASMIEGAADEVLIDRLNYAGKVAGLLRSS
                     GLAPLMSMPRVRAAARELQDILTEKGIPVSILFI"
     gene            complement(7145..>7973)
                     /locus_tag="PKN85_01185"
     CDS             complement(7145..>7973)
                     /locus_tag="PKN85_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43046.1"
                     /translation="KKEEQYLALVESVREWIWETDANFVHTYVSPRVHDVLGYSPEEV
                     IGKSPIDFIPAEERARSLPVLKDLLSRKQPFAAFQTMGRHKGGHSIYIQASGIPYFDE
                     AGNLLGYRGSCHDITEQKNTMDALMEREKELFAKSQTLEEVNAALKVLLKQREEDRKE
                     LEGKFVSNIKEMILPYILKIQKSQRDPRVKAYLDIATANLNEIISPFLNTIRQLNLSP
                     REIEVASFIKEGRTTKEIAQILGVATSAVDSHRNNMRVKLGLNKKKVNLRSYLLSLR"
BASE COUNT         1520 a         2371 c         2090 g         1992 t
ORIGIN      
        1 cctccggcgt gacaggccgg cgttataacc aggcttaact acgaccccgc gtatatggta
       61 ggcggaacag gttttgaacc tgcgaccctc ggcttgtaag gccgatgctc tcccgctgag
      121 ctatccgccc aacagcggtg aatataacag gttcagcatg aaatgtcaac ctctcacgca
      181 ctctcggcct cttcttcgta gataaggatg ggcctttcgt ggttgacgat cacctcttca
      241 cttatgacac attccttgac attggatagt gaaggtatct cgtacatgac atcgagcatc
      301 accttctcca tgatggaccg caaaccccgg gcgccggtct tcttcttcat ggcctccctg
      361 gcgatccctc gcaatgaccc ttcagtgaag gtgagcttga cgttcacgag gtcgaaaagc
      421 ttcttgtact gcttcacaat ggcgttcctc ggcctcttca ggatctccac aaggttctcc
      481 tcggagagtt cctcgagggt tgccgtgatg ggcagacggc cgatgaactc gggtatgagc
      541 ccgtatttca ggaggtcttc gggctggatg tccctgatga gctcggaata catcttgtcc
      601 cttccgccct ccacgtcgac accgaagccg atgctcttct ttcctatcct gcccgcgatg
      661 aggccctcga tgctgtggaa cgcgcccccg cagatgaaga gtatgttcga tgtgtcgacc
      721 ttgatgtagt cctgctgggg atgcttcctg ccgcctttgg gcggcacgtt cgcgacggtg
      781 ccctcgatta tcttgagaag ggcctgctgg accccttccc cggaaacgtc acgggttatc
      841 gacggactgt ccgtcttgcg tgagatcttg tcgatctcgt cgatatacac tatcccccgc
      901 tccgcccgtt ccacgttgta gtcggccgcc tggagcaggt acaggatgat gttctccaca
      961 tcctcgccca cataccccgc ctcggtgagc gttgtggcat ccgctatggt gaagggcaca
     1021 tggagtatct tcgcgagggt ctgggcaagg agcgtcttcc ccgagcccgt cggaccgatg
     1081 agcaggatat tgctcttctg gatctccaca tcatcgaact gagtctttga ctctatcctc
     1141 ttgtagtggt tgtaaaccgc gacggaaagg acctttttcg cgtggtcctg cccgatcacg
     1201 aagtcatcga ggagtttgtt gatctcgtga ggtttcggga tggccgtctt gatgtaatcg
     1261 aaacgctcct tctttgcctc ttcctgaatt atctcgttgc atagctcgat acactcgtcg
     1321 catatgtaaa ccatcgggcc ggctatcagc ttcttgacct cgtcctgact ctttccgcag
     1381 aatgagcagt tgagcttgat ggtcctgccg tttgtcttct tgatcatgtt ctacaacctc
     1441 ttctcaagta tctgatctac tataccatac tctatggctt gctgcgcgtc catgaaataa
     1501 tcgcgctccg tgtccttcgc caccttgtcg aggggctggc cggtgtgtct cacgagaatg
     1561 ttgttgatcg attcccgcat tctcagtatc tcttttgcct ggatgtcgat gtcgcttgcc
     1621 tgcccctgcg ctcctccgag cggctggtgg atgaggatcc gcgagtgagg cagcgcgaag
     1681 cgcttgccct tctccccgcc ggcgaggagc accgctccca tgcttgccgc ctgcccgata
     1741 caggtcgtca cgacgggtgc ctttatgtac tgcatcgtgt catagatggc aagcccgctg
     1801 gtgatgatgc ctcccgggct gttgatgtag agatatatgt cctttgacgg gtcttcggat
     1861 tcgaggaaca ggagctgggc gatgatgatg ttcgccacca tgtcgtcgat cccggtgccc
     1921 agaaagacga tccgctcctt gaggagccgc gagtatatgt cataggcccg ctcgcccctg
     1981 ccgtccttct cgatgactat tggtacaagg ctcattcatt ctccttcggt gcgtccttta
     2041 ccacggcatt cgcgagcacg aagtctatgg ccttctcttc aacgatgttg tccctgagcc
     2101 ctgccacaag gccgtattgc tcgtaaatac ccttgacctc attgtatgtc ttgttggcgt
     2161 cttccgcaat cgttttcaga cgctcctcga cgtccttgtc gtccgccttg atatcttctt
     2221 tctccgcgat gcgggccaga acgatctcgg ccttgatccg gtcttcggcc cgttttccga
     2281 aatcagcgtg cattctttcg gagatggact gcaactcctc ggcggaaagc ctgtccgtct
     2341 ggtagcgcga ctgggcttcc tctatcatcc cgtgcacgcg tttctccagg aatctttttg
     2401 gaacggggat ctccctgtcc ctgagaagca cctcaagggc cttccgggta aggtcgctct
     2461 tgcgaaaacc ttccttttcc tttatcagtt cctccctgac acccttgcgc atgacctcga
     2521 ggttctcgaa gctcatgtcc cgggcgaact cgtcattgac ctcggggagc accttctgct
     2581 tgatctcctt catggtgacc ttgaaaagga tcttcttcga cgcgatgtcc ttgtcgggat
     2641 aatcgtctgg gaaggtcacg tccacgtccc tctcctcgcc ctctttcatg ccgatgacgg
     2701 cattttcgaa ctcgggcatg agcatggcgt tcccgagctc caggggatat ccgtcggccg
     2761 cgatctgttt caaggcctcg ccgtcgcggt atccctgata cttgatgacg acaaaatcgc
     2821 ccttctgagc cgtcgcctcg ccttgcttcg cctcgaagct ggcatggaac tggcgcatac
     2881 tctcaatacg ctgctccaca tccgcatcgg atatgttgat gggctccgcc tcgatctcta
     2941 tacccttgta ctcgggcagc tcgatctcgg gaagaacctc gcagtcaagc gtgtagcctg
     3001 gctcgtcacc ttcgagaaaa tccgccacgg gctctgtgat cggctccaca ttcgcctctt
     3061 tcagagcgtc gaacatggtg ttctgaagca tcctcttccg cgtctcttcc tcgatatatt
     3121 ccttgtaata ctgggtgatg atgggtctcg ggacctttcc ctgcctgaac cctttgatct
     3181 tcgcactcct tcttatctca tcgtagatgg attcccttat acctgagacg gtctcccggg
     3241 gaagaagaac ttcaactttc tttctcacgc tgtcgagcgc ttcaacctga accttcataa
     3301 cacctccgac atgaaaaagg gacttcaaag tccctagaaa actgcactat tataaaccta
     3361 attttgcgaa attagcaata agaatggctt gaatcgcttg aatggcttga atcgcttgaa
     3421 tgttgggaca acccgggtct tgcgtaccat ccgcggggtc tcgatccctc gatgcgaacc
     3481 gggatcctgc ctatcctccc ctcaggccac tcaagcgatt caagcagtct tcacttctgc
     3541 ggtgtctcga ggacggcctg gcagatcagg cgctgggggt ccacctcctc gcgcagtatc
     3601 tggagttctc ccggggtggg ggtttccgcc tccacagcca tggacgtgtc tatggggaaa
     3661 ccggtgctct ccactatctt ctctatcgtt acacccggga aatattccgc gaggtacatc
     3721 tctttcgtct cctcgtgaaa cctgagcacg ccgagattgg tgacgacggc cagggcgccc
     3781 ccgcgcctca ggccgagttc cttccttgaa tccccgccct cgtaccagcc cacactggtg
     3841 agataatcga gtttctcgac aaacctccgt ttctcgtgct gcatgaatgt gataaccccg
     3901 gatgcgaatg tcgccacgtc acaggcaccc ccgcttccgg aaaagcgggt cttcggcctg
     3961 aagtaatcac cgatgcacgt cgtgttgagg tttccgtacc ggtctatctg ggcggccccc
     4021 aggaaggcga ccgtgtggag cttgcgatgg cccactatgg agaaggcctc tatgagcccg
     4081 gaattgagcg acgtaccggc catcactctt aagtccgcca cggccatcgg tatctcgtcg
     4141 agagacgggt cgatgcctcc cgtctcgaag aatatcaccg ctttcggagc aaagatcctc
     4201 ttcgcggcgg tagccgccag tatggacacg cccgtacccg cgaacactat gtcgccgtcc
     4261 tttatgaacc gtcccgccgc cagggccatc atttcattgt cactgtaccg gatcatgaga
     4321 caacctcctt atttcctgtc gagtcccggc gcgtacccga tcaccggatt ggccttgatc
     4381 ttgaggatca tctccattcc caccttcgtg agatagtctt cccaggagcc gaccccgtag
     4441 acccattcgt caagatactc cgtgaagagg gtatcatcct cggccatctt gcgatagagg
     4501 ttaagatgtt tcgggtcgta gtcataaaag ccgtagcatg ccgtcggatg ggcaccgtag
     4561 gggaccttga ctatggcatc cacgaaaaag ggaggcaacg agttctgatc ggggtccgtc
     4621 ctgatgaaag agcgcgggac gatgtcctcg caggtgataa tgaccctgtc cgccgccttg
     4681 gcctgttcga tatcggcaaa ggtcaggccc ttgatccgga ccgtgccgtc atccccgaca
     4741 tactgggcat ggagtatggt gacgtcaggt gtgagagccg gcagaaggac aacctcatcc
     4801 cagtcttcgc tgaaggggtt tgccatcttc gcgtacttcc tgcttgcaac cttctcctct
     4861 ttcctgacct cggccgggaa accttcgtag ttcatgaggt ccgagccaag ccctgacttc
     4921 gtcggaataa agggaatgct cagcgcgcca gcgagaaacc tgagtgacat ctggtagttg
     4981 gaataatcct cgaactggat ctcggaacgc tggatagcct tcttgaacct gacacacgtc
     5041 ggcgcgtacc gcccgttacc gccgtaagct atctccagcc tcccgacgca tcccgccccc
     5101 acaaggacat cgagcgcctg cccgtgggaa tggcagacga ggtggatgtc cttcaccctc
     5161 tgacgcacga tctcgtaggc gaccgccatc ggattgcggg ttatggtaaa accccctatg
     5221 gcaagctggc aaccattttt tacgaaccgt gaaacggcct catcgagacc catcagcctg
     5281 tcagcgatct ctggatacct gttcattcgc tctctccttt acgaatattc gggtcaagat
     5341 catctttgat tcggtggatc aatattcagt tttaggtcga atatgatgct gtggaaagct
     5401 cgcaagctga ttatagcata gaaccatagg gaggcaatac taaattacag ccaatagtta
     5461 cgtcttcgcc gtcgggaaac gggccgttcc tactgcaggg acaggaggta tgaccttaag
     5521 ttcacctttg ccttgccgag acccagtttg ttcctgatat gatggcgatg cagcccgatg
     5581 gcgctgcgtg tcaccttcat gatatcggcg atctgcttcg tcgtcttccc ctcgcggatc
     5641 agcgtggcta cctggatctc cttcggcgtg aagtggtact gtttcgacgt tatcttctgc
     5701 aggaacgacg agaagacgtt cttgatcgtc gtttcgataa tctcgagata ggtctgctgc
     5761 tcaacggtaa gccccgtcaa cttgagttta tccacgtagg gaagaacgag ttccttcacg
     5821 ttggatttga tcctgtcctc aacaagcttt ttatcgtcct ctccgcgggc gaggagggtc
     5881 ttcaaggctt cgttcgtttc ttcaagggcc tgcatcctca tcgccgtctc tttgtctgac
     5941 tttctcgcgg ccgttgttct cggggagtca ttcggtgctg tggatttcat gatggtcacc
     6001 tctcatgatg cattaactgg atgaaagccg aggttcgtta cggctattct caaagtcctt
     6061 atataaatat agggtacgct actttgtcaa gaaaatctta ttaaaaaacc gacaatcggc
     6121 tgaatctggc ctgccgtcgc cctttcagcg cggccccgtg ctccacgggt cggcgccttg
     6181 tggctcacgg gtctgccctg cgggcaaacg ggcacacggg aggatgggtc gccggcgaaa
     6241 ggcaggctcg tttgcgcgcc tcccgcggta cgcagactcc cgaagggttt tgtgatagaa
     6301 taatcggtcc ggcgaaggac gtagccgtca tgaacatacg tgagataacc gcaaggagcg
     6361 cacttacaag aacaggtatc gagggctacg attactgcct gaacccctac gttggctgtg
     6421 gccacggatg caggtactgc tacgcgacct tcatgaaacg gtttacggga cattccgaac
     6481 catggggcga cttcgtcgac gtgaaggcta acgtggccca cgtcctgcgc cggcagctca
     6541 aacgcgtcag ggaaggcacc ctccttatcg gcaccgtgac ggacccctac cagccgctgg
     6601 aaaagaggta ccgactgacc cgggactgcc tgtccgccct cgttccaaca cccctcgagg
     6661 tccatatcct gacccgctcc ccgctcgtcg tcagggacac ggacatcctc aggcaactct
     6721 cccacgtgga ggtcggcctg tcgataacaa cgaaccggga agacgtgaag caggtctttg
     6781 aaccgcgggc cccctccatc gcctcccgcg tcgtcgccct gaaggccctg cacgatgcgg
     6841 gactcagaac atacgtgttt gtaggacctc tgctcccgat ggaccccgga gtccttgcgt
     6901 caatgatcga gggggccgcc gacgaggtcc tcatagacag gctgaactac gccggaaagg
     6961 tggccggtct tcttcgctcc tccgggcttg cccccctcat gtccatgccc cgcgtgaggg
     7021 ctgccgcccg tgaactccag gatatactga cggaaaaagg gatccctgtt tcgatcctgt
     7081 tcatttgagg gtctcccgta tcatctcgtc gccggtcatg gtgtttgtgc ggcagggata
     7141 cgccttacct gagggacaga aggtacgagc gcagattgac ctttttcttg ttgagcccca
     7201 gcttgaccct catgttgttc cggtgcgaat cgacggcgct cgtggccaca ccgaggatct
     7261 gggctatctc cttcgtcgtc ctgccttcct tgatgaaaga ggccacctcg atctcccgcg
     7321 gggacaggtt caattgccga atggtgttga gaaagggcga tatgatctcg ttgagattgg
     7381 cagtcgcgat gtcgaggtag gcctttaccc gggggtcgcg ctgacttttc tgtatcttta
     7441 agatgtaggg aaggatcatc tccttgatat tcgaaacaaa cttcccctcg agttcctttc
     7501 tgtcctcctc tctctgtttc aagaggacct tgagggcggc gttgacctct tccagagtct
     7561 gcgacttggc aaagagttcc ttctcccttt ccatcagggc atccatcgtg ttcttctgct
     7621 ccgtgatgtc atgacagctg ccgcgatagc cgaggagatt gcctgcctca tcaaaataag
     7681 ggatgccgct cgcctgaata tatatcgaat gccctccctt gtggcggccc atggtctgaa
     7741 atgcggcaaa gggctgcttc cgggaaagga ggtccttcag gacaggcagc gaccgggccc
     7801 tttcctccgc ggggatgaaa tcgatggggc tcttccctat cacttcctcg ggactgtacc
     7861 cgagcacgtc gtgcaccctg gggctcacgt aggtgtgcac gaaatttgcg tcggtctccc
     7921 atatccactc tcttacggat tccacaaggg ccaggtactg ctcttccttc ttc
//
LOCUS       DAOQTR010000025         7819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_10276_length_7819_cov_4.090675, whole genome shotgun sequence.
ACCESSION   DAOQTR010000025 DAOQTR010000000
VERSION     DAOQTR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10276_length_7819_cov_4.090675"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="PKN85_01190"
     CDS             complement(<1..1750)
                     /locus_tag="PKN85_01190"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNT43047.1"
                     /translation="MAKSNKFVYFFGGKKAEGNLKLKNLLGGKGAGLADMVNLGIPVP
                     PGFTITTDVCTLFYKAGMKLPKGLEKEVAENIKKVEKIMGAKFGDPRNPLLFSVRSGA
                     RVSMPGMMDTVLNLGLNEKTVEGLAKLTKNPRFAYDCYRRFVQMYGDVVLGLKPANEH
                     EHDPFEVIIDRKKKARKITSDTDLTVDDLRDLVKEFKKAIKQRLGVSFPEDPMEQLWG
                     SIGAVFKSWNTDRAVAYRQLNDIPGDWGTAVNVQCMVFGNMGEDCGTGVAFTRNPSSG
                     DNEFYGEYLINAQGEDVVAGIRTPLPINKKQKTDRSVKSLEEVWPDIYKQVIKIRNTL
                     EKNYRDMQDLEFTIQNKKLWMLQTRTGKRTAFAAFKIAVDMVKEKLITKEEALMRVEP
                     DQLNQLLRPIFDPKEKDKALKAGNLVSKGLNAGPGAASGKVVFNAQDAEAWAARKEEV
                     ILVRIDTSPEDIRGMNASQGILTSRGGMTSHAALVARQMGKVCVAGCGDLDIDYAKKV
                     IRVNGKTIKEGEYVSIDGTTGEVFAGQIKTKPSEVLQVMIDKTLKPSKSQIYQDFALL
                     MKWADEARRLGIRTNAD"
     gene            complement(1760..3835)
                     /gene="glyS"
                     /locus_tag="PKN85_01195"
     CDS             complement(1760..3835)
                     /gene="glyS"
                     /locus_tag="PKN85_01195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNT43048.1"
                     /translation="MSEFLLLEIGTEEIPARFLTPAKEGLSGLIREAFANTRIAFGDI
                     SIQATPRRMALFVQDIAEKQAETVTVKFGPPYNRAFDDAGNPTKAAVGFARSQGVEVG
                     DLTKGVKDGVEFVTVEKEEKGVPTVEMLPVLLPDIISRIPFQKKMRWGAVSFEYARPV
                     QWVLCLFGGTPVTFSVADVTSAPLSWCHRFLSPGPVGIGHPLEYVDALRKNHIIVNED
                     ERLEIIRQGIARIEETTGGKALRDEELVREILYITECPYPLKGSFDAVYLDIPKEVLV
                     NVMKSHQRYIPIEDAAGRLLPHFIFFANTVPKDDANVIRGNEKVLRARLADARFFFDE
                     DVKAKLADRYERLASIVFHVKLGTLRDKMERVKAIAGYLASVVDHGVTGKLERLIPVM
                     KTDLVTHMVGEFPELQGTMGRIYARIEGEDDEVALAIEEHYLPTGGNGSLPRTSTGAI
                     AGIADKIDSIVSFFSVGITPTGNLDPYALRRQSLGIIKIAIDRKMHLPLTTLVERAFE
                     AGGNIPKRLTLEETKASLLEFIATRFKFSMLEENHNQDFVESVLPCVTSDIYDGYMRL
                     VSLETQRSMEDFERLMVGFRRVFNITKQITEEMTVDPALLALEEEKNLFELYAARKDN
                     FYGLMEKRDYDGALAVLVGFKETIDNFFDKVFVMDKDEAVKSNRLALLTHLRNMFLTF
                     ADFSKIRFE"
     gene            complement(3884..4756)
                     /locus_tag="PKN85_01200"
     CDS             complement(3884..4756)
                     /locus_tag="PKN85_01200"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNT43049.1"
                     /translation="MYFQDLIFALQKFWADRGCIVQQPYDMEVGAGTFHPATLLRCLG
                     PEPWNTAYIQPSRRPADGRYGENPNRLQHYYQFQVVMKPSPKDIQNIYIDSLKSFGID
                     TDYHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQAGGITLSPVCVEITYG
                     TERIAMYLQDVDNVYDIRWNENVLYGDVFLEPEREFSIYNFEESDPVMLRTLFDTFEG
                     EGEKLVRNRGLIYPAYDFCLKCSHVFNLLDARGAISVTERANYIARVRNLAKMCAELY
                     VQKREELGFPLLKR"
     gene            4971..5993
                     /gene="dusB"
                     /locus_tag="PKN85_01205"
     CDS             4971..5993
                     /gene="dusB"
                     /locus_tag="PKN85_01205"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNT43050.1"
                     /translation="MLKIGDIELPVPVMLSPMAGVSDLPFRLITRSFGSPLAFTEMID
                     INAISHKDRRTTHMLSSSPDDRPLGVQFLGNSPSQIPLAIESLSGHACDLIDFNAACP
                     SPKVTRKGKGSALMKDPRMLRDILSALVRSTALPVAVKIRAGWDGDSVNARDVALFAR
                     DAGISALFIHGRTRAQGYSGAVDYGVIREVKEALDIPVIASGDNLSVDRVRRMFAETG
                     CDGVAIARGALGNPWIFRDVVRFFRDGTVPPGPDVAERVSVMKRHLELSVSHWGEKRG
                     VGIFHKFFIWYTRGFSGLRPLRDRAFRTGTMEGLIEVIDELTQRPERRGMFSARSRGA
                     PIDPFV"
     gene            6071..7009
                     /locus_tag="PKN85_01210"
     CDS             6071..7009
                     /locus_tag="PKN85_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43051.1"
                     /translation="MARKRRCKIEDRGVRPVWTVLRTAFIIAAAFILSGCIDDTISVT
                     LRTDGSGTIEETVLMGNEFIEMMQGMGKGVGEEGKDRAGQTPVRGDAKNYDAVVTGMM
                     EKAKNNAKDFGEGVSLVSVKPARSDSASGYTAVYSFKDIGKVTLNQNPGSKTPLNDAD
                     KEKDKAEGAGSKDTPGKDRIRFVFTKGSPARLAVHMPPPKPADKDEKEKTAPKEPAKD
                     DPNAIEMMKAIFKGMRVRIALTVDGDIVKTNATHRLGRKITLIEMDFGRLISHAELLK
                     KIDREKPQSIEEMKAALNNVEGLKLEFEDPVVVDFK"
     gene            7051..7215
                     /locus_tag="PKN85_01215"
     CDS             7051..7215
                     /locus_tag="PKN85_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNT43052.1"
                     /translation="MKRTILLAGAFFLLAGCSTVYVKAGKSAEDFEKDREACEVVAKK
                     ELTARGAART"
     gene            7474..>7819
                     /locus_tag="PKN85_01220"
     CDS             7474..>7819
                     /locus_tag="PKN85_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43053.1"
                     /translation="MVRAKLWFRCAAMHDPVTPMVAQPALVGWEAKRRRVDLTIERSF
                     NGEELVRRMKGWVTTDPKEVIEVVRKYGKLKVLDDRELVIEAEKEEDMEKLNKELAEV
                     FGGEVDVEIVKRS"
BASE COUNT         1527 a         2162 c         2485 g         1645 t
ORIGIN      
        1 ggtcggcgtt tgtcctgatg ccgagcctgc gggcctcgtc ggcccatttc atgaggaggg
       61 caaaatcctg atagatctgc gactttgagg gcttcaacgt cttgtcgatc atgacctgaa
      121 ggacttccga gggcttggtc ttgatctggc ccgcgaagac ctctcccgtc gttccgtcga
      181 tggagacgta ctcaccttcc ttgatggtct tcccgttgac cctgatgacc tttttcgcgt
      241 agtcgatatc gaggtcgccg cagccggcca cgcagacttt ccccatctgg cgcgccacga
      301 gggcggcgtg ggacgtcatg ccgccgcggg aggtgagaat accctgggag gcgttcatgc
      361 cgcggatgtc ttcgggggat gtgtcgatgc ggacgaggat gacctcttcc ttccgcgcgg
      421 cccaggcctc ggcatcctgc gcgttgaaga cgaccttgcc ggaagccgcg ccgggacctg
      481 cgttgagtcc tttcgagaca aggttgccgg ccttcaaggc cttgtccttc tctttcgggt
      541 cgaagatggg acgcaataac tggttgagct ggtccggttc caccctcatc agggcctctt
      601 ccttcgtgat gagcttctcc ttgaccatgt caacggcgat cttgaatgcc gcgaaggcgg
      661 tgcgcttgcc ggtgcgcgtc tggagcatcc agagcttttt gttctggatg gtgaactcca
      721 ggtcctgcat gtcgcgatag ttcttctcca gggtgttcct tatcttgatg acctgtttgt
      781 atatgtccgg ccagacctct tcgagggact tgacggatct gtcggtcttt tgtttcttgt
      841 tgatgggcaa cggcgtcctg atgccggcca cgacgtcctc gccctgcgcg ttgatgaggt
      901 actctccgta gaactcgttg tcgcccgatg acgggttcct cgtgaaggcg acgccggtgc
      961 cgcagtcctc gcccatgtta ccgaagacca tgcactgcac gttgacggcg gtcccccagt
     1021 cgccggggat gtcgttgagc tggcggtacg cgacggccct gtcggtgttc caggacttga
     1081 agacggcccc tatggagccc cagagctgtt ccatggggtc ctcgggaaag gacacgccga
     1141 gcctctgctt gatggccttt ttgaactcct tgacgaggtc cctgaggtcg tcgacggtga
     1201 ggtccgtatc ggaggtgatc tttctcgctt tcttctttct gtcgatgatc acctcgaagg
     1261 ggtcgtgctc gtgttcgttc gcgggtttaa ggccgaggac cacatcgccg tacatctgga
     1321 cgaaccggcg ataacagtca taggcgaagc gggggttctt cgtgagcttc gcgaggcctt
     1381 cgacggtctt ttcgttgagg cccaggttga ggacggtgtc catcatgccc ggcatgctga
     1441 cccgggcgcc ggaccggacg gagaagagaa gggggttcct gggatcgccg aacttcgcgc
     1501 ccatgatctt ttccaccttc ttgatgttct cggcgacctc cttctcaagg cctttgggga
     1561 gcttcatgcc ggccttgtag aagagcgtgc agacgtcggt ggtgatggtg aagcccggcg
     1621 gcacgggtat gcccaggttc accatgtccg cgaggccggc acccttcccg ccgaggaggt
     1681 tcttgagctt gaggttgcct tcggccttct ttccgccgaa gaagtagacg aatttattgc
     1741 tttttgccat atttcctcct tattcgaagc gtatctttga aaaatcagca aaggtcagga
     1801 acatgttcct gagatgcgtg agaagtgcca gcctgttcga cttcacggcc tcgtccttgt
     1861 ccatgacgaa gaccttgtcg aagaagttgt cgatggtttc cttgaaaccg acgaggaccg
     1921 cgagggcccc atcgtagtcg cgtttctcca tgagtccgta aaagttgtcc ttccgtgcgg
     1981 cgtagagttc gaagaggttt ttctcttcct caagcgcgag aagggcggga tcgacggtca
     2041 tctcttccgt gatctgcttg gtgatgttga agacgcgccg gaagcccacc atgaggcgct
     2101 cgaaatcctc catggacctc tgggtctcca gggagacgag ccgcatgtag ccatcgtata
     2161 tgtcgctcgt gacgcaggga aggacacttt caacgaaatc ctgattgtgg ttctcctcca
     2221 acatggagaa cttgaaccgc gtggcgatga actccaggag agaggccttc gtctcttcga
     2281 gggtgagcct cttcgggatg ttccctcccg cctcgaaggc cctttcgacg agcgtcgtga
     2341 ggggcaggtg catcttcctg tctatggcta tcttgatgat gcccagcgac tgccttctga
     2401 gggcataggg gtcgaggttg cccgtggggg tgatcccgac ggagaagaag gagacgatgg
     2461 agtcgatctt gtccgctatg ccggcgatgg cgcccgtcga tgtgcggggc aggctgccgt
     2521 tgccgcccgt gggaaggtag tgctcctcta tggcgagggc cacctcgtcg tcctctccct
     2581 cgatgcgcgc gtagatgcgt cccatcgttc cctgcaactc ggggaactcc ccgaccatgt
     2641 gggtgacgag gtccgttttc atcacgggga tgagcctttc aagctttccg gtgaccccgt
     2701 ggtcgacgac ggacgcgagg tatccggcga tggccttcac cctctccatc ttgtccctga
     2761 gggtgccgag cttgacgtgg aagacgatgg aggcaagccg ctcgtacctg tcggcgagct
     2821 ttgccttcac gtcctcgtcg aagaagaagc gcgcgtcggc aaggcgggcg cggagcacct
     2881 tctcgttccc ccgtatgacg ttcgcgtcat ccttcgggac cgtgttggcg aagaatatga
     2941 agtgaggcag gagccgtccc gcggcatctt cgatgggaat gtaccgctgg tggctcttca
     3001 tgacgttgac gaggacctcc ttggggatat cgaggtacac cgcgtcgaag gagcccttca
     3061 ggggataggg gcattcggtg atgtagagta tctccctcac gagctcctcg tcccggagcg
     3121 cctttccgcc cgttgtctcc tcgatgcggg cgatcccctg ccgtatgatc tccaggcgtt
     3181 catcttcgtt cactatgatg tggttcttcc tgagggcgtc gacgtattcc aggggatgac
     3241 ctatcccgac cgggcccggc gacaggaagc ggtggcacca gctcaggggc gcactcgtga
     3301 catcggccac ggagaacgtg acgggcgtgc ccccgaagag gcacaggacc cactggacag
     3361 gccgcgcata ttcgaaactc acggctcccc agcgcatctt cttctgaaag gggatacggg
     3421 agatgatgtc gggcaggagg acggggagca tctccacggt ggggaccccc ttttcctctt
     3481 tctcgacggt cacgaattcc acgccgtcct tgacgccctt cgtgagatcg ccgacctcaa
     3541 cgccttggga acgggcgaag ccgacggcgg ccttcgtggg atttcccgcg tcgtcgaagg
     3601 cgcggttata cgggggccca aacttgacgg tgaccgtctc cgcctgcttc tccgcgatat
     3661 cctggacgaa gagtgccatg cgccgcgggg ttgcctggat gctgatgtcg ccgaaggcga
     3721 tccgcgtgtt ggcgaaggcc tcccgaatga ggcccgaaag cccctctttc gcgggcgtga
     3781 ggaagcgcgc cggtatctcc tctgtgccta tctccaaaag cagaaattcg ctcatgatgt
     3841 tattcccttg ccgtgttccc gccgccccgc gcggggtgac ccgctatcgc ttgagcaggg
     3901 ggaaaccgag ttcttcacgc ttctgcacgt acagttccgc gcacatcttc gcgaggttcc
     3961 tcacccgcgc aatatagttg gcgcgttccg tcacgctgat ggcgccgcgg gcgtcgagaa
     4021 ggttgaagac atgcgagcat ttcaggcaaa aatcgtaggc cgggtagatg agcccccggt
     4081 tcctgacaag cttttcacct tccccttcga aggtgtcgaa aagagttctg agcatgacgg
     4141 ggtccgattc ctcgaagttg taaatggaga attccctttc gggttcgagg aagacatcgc
     4201 cgtagaggac gttctcgttc cacctgatgt cgtaaacgtt gtcaacgtcc tggaggtaca
     4261 tggcgatgcg ctcggtgccg taggtgatct ccacgcacac aggcgacagg gttataccgc
     4321 cggcctgctg gaaataagtg aactgcgtga tctccatgcc gtcgagccat acctcccagc
     4381 cgaggcccca ggcgccgagc gtcggcgact cccagtcgtc ctcgacgaac cggatatcgt
     4441 gatagtcggt gtcgatgccg aaactcttca agctgtctat atagatgttc tgaatgtcct
     4501 tgggagaggg tttcatgacg acctggaact ggtaatagtg ctggaggcgg ttggggttct
     4561 ccccgtagcg cccgtccgcg ggccgccggg acggctggat gtacgcggtg ttccagggtt
     4621 ccggtccgag acagcgaagg agtgtggcgg gatggaacgt gcccgccccg acctccatgt
     4681 cgtacggctg ctggacgatg cagcccctgt cggcccagaa tttctgcaat gcgaatatga
     4741 ggtcctgaaa gtacatgccg gaaaaatcca cgttttctat accacaaaag aaggcgattt
     4801 gccaatgatt ttagtgcacc atgcgcgcaa gggggaaaag gggaaggact gatggaaggg
     4861 tatgggacct ataggaggta taggacctat aggacgaggg cgggacgtgt gggggttgag
     4921 atgcggtggg ggtgtttgac actcttggtt ggtgcgtcat agaataagcc atgctgaaga
     4981 tcggtgatat cgagcttccc gttcccgtta tgctttctcc catggcgggg gtgagcgatc
     5041 tgccttttcg gctcatcacg cggtcatttg gttcgcccct tgcctttacg gagatgatag
     5101 atatcaatgc gatatcccac aaggacaggc ggaccaccca catgctgagt tcttcgcccg
     5161 atgacaggcc tcttggcgtg cagttcctgg gcaacagccc gtcgcagata ccgctcgcca
     5221 tcgagagcct ttccgggcat gcctgcgacc tcatcgattt caatgcggcc tgtccctctc
     5281 cgaaggtcac acgcaagggc aagggctcgg ccctcatgaa ggacccgagg atgctccgcg
     5341 acatcctttc cgccctggtg cggtcgacgg cgctgcccgt cgccgtcaag atccgcgcag
     5401 gatgggacgg cgattccgtc aacgccaggg atgtcgcgct ctttgcccgg gacgcgggca
     5461 tcagcgcgct gttcatccat ggcaggacga gggcgcaggg gtacagcggc gccgtcgact
     5521 acggggttat cagggaggtc aaggaggctc tcgacatccc cgtcatagcc tcgggagaca
     5581 acctgtccgt cgacagggtc cgcaggatgt tcgccgagac cggctgcgac ggcgtggcca
     5641 tcgcgcgcgg ggccctcggc aacccctgga tattcaggga tgtcgtgcgt ttctttcggg
     5701 acgggacggt gccgccggga ccggacgtgg cggaacgtgt ctccgtcatg aagcgccacc
     5761 tcgaactcag cgtctcgcac tggggcgaaa aaaggggcgt cggcatcttc cacaagttct
     5821 tcatctggta cacgaggggc ttttcgggcc tgcgccccct tcgggacagg gccttcagga
     5881 cgggcacgat ggaaggcctc atcgaggtca tcgacgagct cacgcaaaga cccgagagac
     5941 gagggatgtt ttcggcacgc agccgcgggg cgcccatcga cccctttgtc tgaaattgtc
     6001 ttgactgacc tgtcaagggg ttggtagcat tgaccatgtt ttcctgagtc ccgtccgcgc
     6061 gggaggagga atggcgcgca agaggcggtg caagatcgaa gacagggggg tccgtccggt
     6121 atggaccgtg ctaaggaccg ccttcatcat agcggcggca ttcatccttt cgggttgtat
     6181 cgacgatacc atctccgtca cgctgaggac cgatgggagc ggcaccatcg aagagaccgt
     6241 cctcatgggc aacgagttca tagagatgat gcaggggatg ggcaaaggtg tcggcgagga
     6301 aggcaaggac agggccgggc agacccccgt gcggggcgat gcgaagaact acgatgccgt
     6361 cgtgacggga atgatggaaa aggcaaagaa caacgcgaag gatttcgggg aaggggttag
     6421 cctcgtctcc gtcaagccgg cgagatcgga ttcggccagc ggctataccg cggtgtactc
     6481 tttcaaggat atcggcaagg tcacgctgaa ccagaacccg gggagcaaga cgccccttaa
     6541 cgacgcggac aaggagaaag acaaggccga gggtgccggg agcaaggata ctcccggcaa
     6601 ggacaggatt cgtttcgttt tcacgaaagg gtcgcccgcc aggctcgccg ttcatatgcc
     6661 tcccccgaaa ccggcggaca aggacgaaaa ggaaaagacg gcgccgaagg aaccggcaaa
     6721 ggatgacccc aatgccatcg agatgatgaa ggccatcttc aagggcatga gggtccgcat
     6781 cgccctgacc gtggacggcg atatcgtgaa gacgaacgcc acccatcgtc tggggcggaa
     6841 gataaccctc attgagatgg atttcggcag gctcatcagc catgcggaac tcctcaagaa
     6901 gatcgacagg gagaaaccgc agtctatcga ggagatgaag gcggcgctga ataacgtcga
     6961 aggactcaag ctggagtttg aggatcctgt cgtcgtcgac ttcaagtgac ggaccttcat
     7021 aggaacaaca aactaaggag tcggagaaca atgaaaagga ccatattgct cgcgggcgcg
     7081 ttcttccttc ttgcagggtg ctcgacggtg tatgtgaagg cggggaagag cgccgaggat
     7141 ttcgaaaaag acagggaagc ctgtgaagtc gtcgcgaaaa aggagctcac ggccaggggc
     7201 gccgcccgga cctgaagcgc tgtttttgaa gagacaaggc gttgtctcga aaagaagggt
     7261 tggagaccga agggctaagg cgcatacggg cagatggggg aaaccgcgtc tgcccaaaat
     7321 agatttgcgg cagcggacag acagactctc cgggacatct cccggagggg gataatgacc
     7381 cgtgtgagcg tgtgcccatt tccttgactt tgcacagaca aaccattaat ttaaggtttg
     7441 attttcaaag aatcggacag gaggcttgaa tcaatggtca gggcaaaatt gtggttccgg
     7501 tgtgcggcga tgcacgatcc cgtgaccccc atggtcgccc agcccgcgct tgtcggatgg
     7561 gaagcgaaga ggcgcagggt ggacctgacg atcgaaaggt ctttcaatgg ggaagaactg
     7621 gtcaggcgca tgaaggggtg ggtgaccacg gacccgaagg aggtcatcga ggtggtcagg
     7681 aaatacggca agctcaaggt cctcgatgac cgggagctcg tgatagaggc cgaaaaggaa
     7741 gaggacatgg agaagctcaa caaggagctt gccgaagtct tcggcggcga ggtcgatgtg
     7801 gagatagtga aaaggtctc
//
LOCUS       DAOQTR010000026         7743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_10393_length_7743_cov_3.303330, whole genome shotgun sequence.
ACCESSION   DAOQTR010000026 DAOQTR010000000
VERSION     DAOQTR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10393_length_7743_cov_3.303330"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKN85_01225"
     CDS             complement(<1..334)
                     /locus_tag="PKN85_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT43054.1"
                     /translation="MGRKTERSGRRRCPAGPEGFTLLEILVAFVILATVVTVVLQLFS
                     ANLTTLSMSDDYLTATVLADIRMRELLEGGQLKEGTSSETKDGYVMDMTVTETLKERT
                     KGFTWQVFE"
     gene            complement(318..773)
                     /locus_tag="PKN85_01230"
     CDS             complement(318..773)
                     /locus_tag="PKN85_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT43055.1"
                     /translation="MKVNGNGFTLLELVIVMTLMVLFLGLSGVHFANRLPSAKLDGTA
                     RELSGLIRMARIEAMSIREERRVTIDLNRRTYGLKGAARRAVPEDVAISVVDPVRGAV
                     YGGEYDIIADASGTVGSCEIVLGTKKKTLRIEIDPVIGSVVVKGYGKEN"
     gene            complement(770..1960)
                     /locus_tag="PKN85_01235"
     CDS             complement(770..1960)
                     /locus_tag="PKN85_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT43056.1"
                     /translation="MYSYKATTLEGAIIEGTIEAPDENLALERIKDWGAIPLKVSSPG
                     KGVLKKGVSLHSSKGDLLTFTAELSALIGAGLPLDRSLNILAGVSEGRQMQEVVNSVL
                     RLIREGGSFSDALKKHPRVFPRLYVNMVRAGETGGVLDIVLEKLGEFLETAKELKDSV
                     YSAMIYPVILGVTGSISIVILLTFVIPRFSILFKEMGGTIPASTQMILYFSNFLRDLW
                     WILLPAAVSGAFVVWRFVKTERGGILWDTIKFRFLRDVVSKLETARFCRTMGTLIKSG
                     VPFLQALQNSKDVIGNRVVAAAIGAVARDVKEGKGIAVPLTEAKVFPQLALSMIKVGE
                     ETGQLDTMLLKVATTYEKSLKDSVKRFMGLLEPAIILTMGLVIAFIVVSMLVAIFGIL
                     ELPF"
     gene            complement(1967..3499)
                     /gene="gspE"
                     /locus_tag="PKN85_01240"
     CDS             complement(1967..3499)
                     /gene="gspE"
                     /locus_tag="PKN85_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNT43057.1"
                     /translation="MNGKTIIHLSPEEFPRVPLVMDGIATRFIRENKIIPFEWKDGTL
                     KIIMADPSKRELVDALRAATASDVLVHSGDPAVIDEHISRFYTKDQQNLDKIIEGIDE
                     REGYEFIRDEEEEDVGHLKDLASEAPIIRLVNLFITRAVELRASDIHIEPFEDEMKIR
                     YRIDGVLNDVESTPKRLQAAIVSRIKIMARLNIAERRLPQDGRIRLRVGEKEIAIRVS
                     TIPIVNGESVVMRLLDRESIVIDLDKLGFSQAMLMSLDRLITKPNGIILVTGPTGSGK
                     TTTLYGALDKINSPEKKIITVEDPVEYQLKGVNQIQVKPQIGLDFNSTLRHIVRQDPD
                     IIMIGEIRDLETAQIAIQSALTGHLVFSTLHTNDAPSAITRLLDMGVESFLLSSTVRG
                     ILAQRLVRMICPDCRRQTGVSDDVEELMALGMGGGVPVWRGAGCEACGHTGFFGRSGL
                     FELLVVDEDLRRLTLKGADEGEIRAQARRSGMKTLLEDGMEKIRMSVTTLDEVFRVTR
                     ED"
     gene            complement(3496..5541)
                     /locus_tag="PKN85_01245"
     CDS             complement(3496..5541)
                     /locus_tag="PKN85_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNT43058.1"
                     /translation="MPSRQTPEASAPKRTVGGQSALRSAATSPSAKDENTEKEEQTAG
                     DRGKISPPGNPSAPPGRIPSQAKKPGARAASPVSLSFDDADMYQVIQTVFGEILRVNY
                     IVDQRVKGRVTFRSVTPVEGDRVLSVMEVILRLNGVGVVEEGGLYRIVPISDVAREPA
                     EVTVGRNPDSIEVEGRSIIRVVPVMYGQSSEIVKLITPFLTTTAVIIDVPNINHIMLV
                     DTDANVKRLLKLIDLFDSEQTRKRKPQVFVYHVQNSKAKDIANILQQVFLSSGRGGAA
                     NPPLAALPSAGKTAGASGTPAARSGPEGGLIVSGSQAGEGLISPVTKIIAEENLNSLI
                     ILSLPEDYEVIKEAIRRVDIIPRQVIIEGVIAEITLKDDLKLGVSWALQFSPDGMNGV
                     LGAVDGTVGFDVPGATATSTTGSGTFTFAGTVGGDFKAVIDTLATQSKAKLLAVPRIL
                     VTDNKEARIQVGEQVPIVTTETIASTTTTAQRTIQYKDIGIILKVKPRINEGGLVTLD
                     LAQEVSSYDTITLFDGETNIIVKKTEATTNLVVQDGQTILIGGLIREDISAARSGIPY
                     LSKIPLLGYLFGSTTDENHRKELIILLTPRVIKNQHDARKATSSYVDDITKTGKGKLK
                     KEELIRDVPRQEKDGKNDAGETREGRGTKEHEATHTEVPSAGEEPAAKAAAGSTGES"
     gene            5931..6362
                     /gene="gspG"
                     /locus_tag="PKN85_01250"
     CDS             5931..6362
                     /gene="gspG"
                     /locus_tag="PKN85_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNT43059.1"
                     /translation="MTIQYSYCTDRRGFTLVELLVVMVIVGLLVALVGPKVFPKLGKG
                     KQAAAKAQVELLSQTLDHFRLDVGRYPTTQEGLTALVTNSGGTQNWEGPYLKKALPND
                     PWGKPYQYQSPGSHGEFDLYSYGRDGTPGGEGEDKDVANWE"
     gene            complement(6501..>7743)
                     /locus_tag="PKN85_01255"
     CDS             complement(6501..>7743)
                     /locus_tag="PKN85_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HNT43060.1"
                     /translation="DAKNSYLNMRSNFKKAGARGTRGNAASAIDDAYSNSSSDDMVAV
                     VDELFTLDDGALNSALDQISGTTHSVSSTVTPAKQGAFYQNLFGRTGGTTGLGFLGLN
                     DRQSDPLTMLASGGPVASDAGFTLTRDGQKLPYGMWIKGYSVTGNRHGDDVGSRYDYT
                     IGGAIAGFDYLINPNIRAGVAVGYSKTNVDMKQLRDNSDAESWQGAIYGSYVPTSKLW
                     YVDAAFAYSKNSYETKRYLNFGNIYRVARGSYDGSDISGYLEGGYKVPIFGGITATPL
                     ASFLAMCNHTDSFTETGAGALNLESDSNHTDSYQSGLGVKLSREFKAAKDFTLTPEFG
                     AKWLHEFGDTEANINARFAGEPAGSFVINSSTAKRDTGVFSLNLTGKKSDMLNFFVGY
                     DLGITSDQISHGFTGGLRFNW"
BASE COUNT         1535 a         2334 c         2146 g         1728 t
ORIGIN      
        1 cctcgaacac ctgccacgtg aaacccttcg tgcgctcttt gagcgtctcc gttacggtca
       61 tatccatcac atagccatcc ttcgtctcgc ttgaggtgcc ctccttcaac tgcccaccct
      121 caagcagttc cctcatcctt atatcagcaa gcacggtcgc cgtaaggtag tcgtcggaca
      181 tcgagagcgt cgtgagattc gccgaaaaca actggaggac gacggtgacc acagttgcaa
      241 gaataacgaa ggccacaagg atctcgagga gggtgaaacc ctccgggcct gcgggacaac
      301 ggcgccttcc ggatctttca gttttccttc ccataaccct tcacgaccac ggacccgatc
      361 acaggatcga tctcgatcct gagggtcttc tttttggtac cgagaacgat ctcgcatgac
      421 ccaaccgttc ccgacgcatc ggcgatgatg tcgtattctc ctccatagac cgcgcccctg
      481 acagggtcca ccacgctgat ggcgacatcc tcaggaaccg cgcggcgggc cgctcctttg
      541 aggccgtatg tcctgcggtt aaggtcgatc gtcacgcgcc gctcttccct tatgctcatc
      601 gcctctatgc gcgccatgcg gataaggcct gaaagctccc gcgccgtacc gtcaagcttc
      661 gctgagggaa gacggttcgc gaaatgcacg cccgacaggc caagaaacag caccatgagg
      721 gtcatgacga tcaccagctc gagaagcgtg aaaccattac cgttgacctt caaaagggca
      781 gctccaggat accgaatatc gcaacgagca tggagactac aatgaaagcg atgacgagcc
      841 ccatggtaag gatgatagcg ggctcaagaa gacccatgaa ccgcttgacc gagtctttca
      901 ggctcttctc gtaggttgtc gccaccttga gcagcatggt atcgagctgc cccgtctctt
      961 cgcctacctt tatcatggac aacgccagct gagggaagac cttcgcctcg gtgagcggca
     1021 cggcaatgcc cttgccctcc ttcacgtccc ttgccaccgc accgatcgcg gcagccacga
     1081 cgcggttccc gatgacgtct ttggagttct gcagggcctg gaggaaggga acgccgcttt
     1141 ttatgagggt ccccatggtc ctgcagaatc gggccgtttc gagcttgctc acgacgtccc
     1201 tgaggaacct gaactttatc gtgtcccaaa ggatcccgcc ccgctcggtc tttacaaacc
     1261 gccatactac aaacgctccc gatacagccg ccggaagaag gatccaccag aggtccctca
     1321 gaaagttact gaagtaaagg atcatctgcg tggacgcggg aatcgtcccg cccatctctt
     1381 tgaagagaat ggagaagcga ggtatgacga aggtaaggag gatgacgatc gaaatactcc
     1441 ccgtgacgcc gaggatcacg gggtagatca tggccgagta gacactgtct ttcagttctt
     1501 tcgctgtttc gagaaactca cccagctttt cgaggacgat atccagaaca ccgcccgttt
     1561 caccggcccg gaccatattc acatagagcc tgggaaaaac cctgggatgc ttcttcaggg
     1621 catcggaaaa ggaaccccct tcccgtatga gtctcaggac ggaattgact acctcctgca
     1681 tctgcctgcc ctcggagacc ccggccagga tattgaggct ccggtcgagg ggaaggccgg
     1741 cgccgataag cgccgacagc tctgcggtga aggtgagaag atcgcccttc gatgagtgca
     1801 gcgacacgcc ctttttgagg accccctttc ccggtgacga aaccttcaaa gggatggcgc
     1861 cccagtcctt gatcctctcc aaggccaggt tttcatcggg cgcctcgatg gtgccttcga
     1921 tgattgcgcc ttcaagggtt gtcgccttgt atgaatacac tcccaatcaa tcctccctcg
     1981 tcacccgaaa gacctcgtcc agggtggtca cactcatgcg gatcttctcc atcccgtcct
     2041 cgagaagcgt cttcatgccc gaccttctcg cctgcgccct gatctcgccc tcgtcggcac
     2101 ccttcaaggt cagccgcctc agatcttcat cgacaacgag aagctcgaag agccccgagc
     2161 gcccgaagaa ccccgtatgt ccgcaggcct cgcagcctgc tccccgccag acgggcacgc
     2221 cgccgcccat gccgagggcc atgagttcct ccacgtcgtc ggatacacct gtctggcgcc
     2281 tgcaatcggg gcagatcatc ctcacgagcc tctgcgcgag gatgcccctg accgtggatg
     2341 acaggagaaa gctctccaca cccatgtcaa gaaggcgtgt tattgcgctc ggcgcgtcgt
     2401 tggtgtggag cgtcgagaat acgagatgcc ccgtcagtgc cgactggatg gctatctgag
     2461 cagtctccag gtcccggatc tcgcctatca tgataatgtc cgggtcctgc ctgacgatgt
     2521 ggcgcaacgt gctgttaaaa tcgagcccga tctggggctt gacctggatc tggttgacac
     2581 ctttcagctg gtactccacg gggtcctcca cggtgatgat cttcttttcc ggcgagttta
     2641 tcttgtcgag tgccccgtag agggtcgttg tcttaccgct gcccgtcggc cccgtcacaa
     2701 ggatgatgcc gttaggtttc gtgatgagcc tatcgaggga catgagcatg gcctgtgaaa
     2761 aacccaattt gtcaaggtca atgacgatgc tttccctgtc gagcagcctc atgacgacgc
     2821 tctctccgtt cacgatgggg atggtggaaa ctctgatggc gatctccttc tcgcccacgc
     2881 gcagccgtat ccttccatcc tggggaagcc tgcgctcggc aatgttgagc ctggccatga
     2941 tcttgatgcg cgagacgatg gccgcctgaa gtctcttcgg tgtcgattcc acgtcattga
     3001 ggacgccgtc tatgcggtag cggatcttca tctcgtcttc gaaaggctcg atatggatgt
     3061 cgctcgccct taactccacg gcacgggtga tgaagaggtt cacgagcctg atgatgggtg
     3121 cctccgacgc aaggtccttg aggtggccta cgtcctcctc ttcctcatca cgtatgaact
     3181 cgtatccctc ccgttcgtcg attccctcta tgatcttatc gaggttctgc tggtccttcg
     3241 tgtagaaccg ggagatgtgc tcgtcgatga cggcggggtc cccgctatgg accaggacgt
     3301 cgcttgcggt ggccgccctc agcgcgtcga caagctcccg cttggaagga tcggccatga
     3361 tgatcttgag cgtaccatcc ttccactcaa aggggatgat cttgttctcc ctgatgaaac
     3421 gcgtcgctat gccgtccatg acaagaggca ccctgggaaa ctcctccggg gagagatgaa
     3481 ttatcgtctt gccgttcacg actctcctgt gctccccgcc gccgccttcg ccgctggttc
     3541 ctctccggcc gacgggacct ctgtgtgcgt cgcctcgtgt tcctttgttc cccgcccttc
     3601 cctggtttcg ccggcatcgt tcttcccgtc cttctcctgc cgggggacgt cgcggataag
     3661 ctcttccttc ttcagcttgc ccttgcccgt cttcgttata tcgtcaacat aggaggacgt
     3721 ggccttccgg gcgtcgtgct ggttcttgat gacccgcggg gtgagaagta tgatcagctc
     3781 cttacggtga ttttcgtccg tggtactgcc gaagagatat ccgagaagcg gtatcttgct
     3841 gaggtacgga atgcccgatc gtgccgcaga tatgtcctcc ctgataagcc ctccgatgag
     3901 gatcgtctgg ccgtcctgaa cgacaaggtt cgtcgtcgcc tccgtctttt tcacaatgat
     3961 gttggtttcg ccgtcgaaaa gggtgatagt gtcgtaggag gagacctcct gggcaaggtc
     4021 aagggtaaca agcccgcctt cgttgatacg gggttttacc ttgaggatga taccgatgtc
     4081 cttgtactgg atggtccgct gagcggtggt ggtggtggat gctatcgtct ccgttgtcac
     4141 gatggggacc tgttcaccca cctggatccg agcctccttg ttatccgtca ccagtatgcg
     4201 cggtacggca agaagcttcg cctttgattg cgtcgcgagc gtatcgatga ccgccttgaa
     4261 gtcacctccg acggttcccg caaaggtgaa tgtcccgctg cctgttgtgc tcgtcgctgt
     4321 cgcgccgggc acatcgaatc cgacggtgcc gtccaccgcc cccagcaccc cgttcatacc
     4381 gtccggcgag aactgaaggg cccaggaaac gccaagcttc aggtcatcct tcagggtgat
     4441 ctccgcgatc accccttcga taatgacctg cctcgggata atatcgacgc gccggatggc
     4501 ctccttgatg acctcgtaat cctcgggcag gctaaggata atgagggagt tgaggttctc
     4561 ctcggcgatg atcttcgtta cgggagaaat aaggccctct cccgcctggg agcccgacac
     4621 gatcaagccg ccctccggcc cgctgcgcgc ggccggggtg ccggatgcgc ctgccgtctt
     4681 ccccgcggaa ggcaacgcag cgaggggtgg atttgctgcg ccgcctcggc cggaggaaag
     4741 aaaaacctgc tgcagaatgt tcgctatgtc tttagctttg ctgttctgga catgatagac
     4801 aaagacctgg ggtttccttt ttcttgtttg ttcgctgtcg aaaagatcga tgagcttgag
     4861 cagtctcttt acgttcgcgt cggtgtcgac aagcatgatg tgattgatgt tgggaacatc
     4921 gatgatcacc gcggttgtcg tgagaaaagg cgtgatgagc ttgacgatct ccgacgactg
     4981 accgtacatg acgggcacga cgcgtatgat cgatctgcct tccacctcga tggaatcggg
     5041 gttcctcccg acggtcacct ccgccggttc tctggcaaca tcgcttatcg gcacaatacg
     5101 gtaaaggccg ccttcctcca caacgcccac cccattgagt ctcaggatga cctccatgac
     5161 ggacagcacc ctgtcaccct cgacaggggt aacggaacgg aacgtgaccc gtccctttac
     5221 cctctggtca acgatgtaat tgaccctcag gatctcgccg aagacggtct ggatgacctg
     5281 gtacatgtcg gcgtcatcaa aggaaaggct gacggggctc gcggcccttg cgccgggctt
     5341 cttcgcctgc gaaggtatcc ttcccggagg cgcggacgga ttccccggag gcgagatctt
     5401 tcctctatca ccggccgtct gctcctcttt ttccgtattt tcgtcctttg ccgacggaga
     5461 cgtcgcggcg gaacgcagtg ccgactggcc gcccactgtt cgcttcggcg ccgacgcttc
     5521 gggtgtctgc cgggagggca ccgcctcccg gcgattgtcg tcggcatgca aaggcggtac
     5581 gacaatcgca aaaaagagac acgacaagaa tatgagcttt ctgacatttg tcatcgattc
     5641 atcacccgct gtagtttttc caggaccaat aggattctag agttaaccgg gacaagttca
     5701 catcgtaatg ggattaaact gacacattat ggtttcaaca aggcgagtgc cattccaagc
     5761 cggcagcagc gaccgacgtg ggggagagcc aaacccggaa atagtaccaa cacgcaccac
     5821 catgtgacga acaggccggt cggggtagtg acaaaccaac gcccgccgga ttagtctctt
     5881 ttcaaaacgc tacagccggt atcaagacac ggcgcattga aggaggactc atgacaattc
     5941 agtattcata ttgcacagac agacgcggct tcaccctggt tgaactcctc gtcgtcatgg
     6001 tcatcgtcgg gctcctcgtg gccctcgtcg gccccaaggt atttccgaaa ctgggcaagg
     6061 gcaaacaggc cgcagccaaa gcgcaggtcg agctcctttc gcagaccctc gaccatttcc
     6121 gccttgacgt cggccgttac cccaccacac aggaaggcct cacagccctc gtcacaaact
     6181 ccggaggaac tcagaactgg gagggtccct acctcaaaaa ggcccttccc aacgatccct
     6241 ggggcaaacc ctaccagtac cagtctcccg gttcccacgg agaatttgat ctctattcct
     6301 acggcaggga cggtacgccc gggggggaag gtgaagacaa ggacgtggcc aactgggagt
     6361 aaaagaacgg tacgcgaggg gcaattaaat aatgatgggc ggccttgagg gccgcccatc
     6421 atttgttatc ttcccaattc atcggttacg tttcatttag ttttcccata acccataacc
     6481 tctgacccat cacccgtctt ctaccagttg aacctcagtc cccccgtgaa gccgtggctt
     6541 atctggtcgc ttgtaatgcc cagatcgtag cccacgaaga agttgagcat gtcactcttc
     6601 ttgccggtga gattcagaga gaaaacaccc gtatctctct ttgcggtgct ggaattgatg
     6661 acgaaggagc ctgccggctc gcctgcgaac cgtgcgttga tgttcgcttc cgtgtcgccg
     6721 aactcatgaa gccatttggc gccgaattcc ggggtaaggg tgaagtcctt tgctgccttg
     6781 aactcccggg acaacttcac acccagaccg ctctggtacg aatcggtgtg gttactgtcg
     6841 gattcgaggt tcaaagcacc tgcgcctgtt tccgtgaagc tgtccgtgtg gttgcacatg
     6901 gcaaggaagg aggcaagggg tgttgcggtg atgccgccga agattggaac cttgtagccg
     6961 ccctcgagat aacccgatat gtcgctgccg tcataggagc cgcgggccac ccggtagatg
     7021 ttgccgaagt tgagatagcg cttcgtctcg tagctgttct tgctgtaggc aaaagccgcg
     7081 tccacgtacc acagtttgct cgtcgggacg taggagccgt atatcgcccc ctgccagctc
     7141 tctgcgtcgg agttgtcccg aagctgtttc atgtcgacat tcgtctttga atacccgact
     7201 gccacgccgg cccggatatt ggggttgatg aggtagtcga aacctgcgat ggccccgccg
     7261 atggtgtagt catagcggga gcctacgtcg tcgccgtggc ggttgcctgt gacgctgtat
     7321 cctttgatcc acattccgta gggcagcttc tgcccgtctc ttgtcagggt aaaccccgcg
     7381 tcggatgcca ccgggccgcc cgaggcaagc atcgtcaggg gatccgactg gcggtcgttg
     7441 agcccgagga agccaaggcc cgtagtgccg ccggtacggc cgaagaggtt ctgatagaag
     7501 gccccctgct tcgcgggagt aacggttgat gatacgctgt gggtcgttcc cgatatctgg
     7561 tcgagggcgg agttgagagc accatcgtcg agagtgaaca gctcatcgac aacggccacc
     7621 atgtcatcgg aggaggaatt gctgtaggca tcgtcgatcg ccgacgctgc gttgcccctt
     7681 gtgcccctgg cccctgcctt cttgaaattg gacctcatat tgaggtagct gttctttgcg
     7741 tca
//
LOCUS       DAOQTR010000027         7665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_10544_length_7665_cov_4.074244, whole genome shotgun sequence.
ACCESSION   DAOQTR010000027 DAOQTR010000000
VERSION     DAOQTR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10544_length_7665_cov_4.074244"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2354
                     /gene="leuS"
                     /locus_tag="PKN85_01260"
     CDS             <1..2354
                     /gene="leuS"
                     /locus_tag="PKN85_01260"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNT43061.1"
                     /translation="PSGKIHMGHVRNYSIGDVIARYMTMKGLNVLHPMGWDAFGMPAE
                     NAAIERGIQPATWTHENIDYMREQLKRLGFGYDWRREVATCDVDYYRWEQWMFLKMFE
                     KGLVYRKSSPVNFCPRCQTVLANEQVEGGNCWRCGEEVIQKELTQWFFAITKYADELH
                     EYCDKLPGWPEKVTSMQKNWIGKSYGVEVHFTLEDGSPFTIFTTRPDTLYGVTFMVLA
                     PEHPLTLELSLGTSQEKEVAAFVQKVKAQDRSFRGEISLEKEGVFTGKYAINPLTGHR
                     IPIYVGNFVLMEYGTGAIMAVPAHDQRDFEFAKEYGLPVIVTITPKDRELNAASMDCA
                     YVDEGVLVNSDRFDGMNNREAIGAIIDYLEEKGFGTRTVNYRLKDWGISRQRYWGAPI
                     PIIYCDDCGTVPVPEKDLPVVLPVDLEIRMVGRSPLADHAPFYEVECPVCKKKARRET
                     DTMDTFVESSWYFLRYACADYQDGPLDRKRVDYWMPVDQYIGGIEHAVLHLLYSRFFN
                     RVLNELGLVGVREPFKNLLTQGMVIKDGAKMSKSKGNVVDPNYLIEKYGADTARLFCL
                     FAAPPERDLDWSDQGVEGSYRFLQRVWRLAAEHAGSLAAVTAVDQIPGAGGDEKQLIH
                     KIHRTIMKVTDDVERFHLNTAIASIMELVNMTYKFVEKGMDGERGKALLKGSIEAILR
                     LLYPFVPHIVSELWQTMGGDVVLLRSSWPSWDAELVKEEKVMIAVQVNGKLRDTCEAD
                     RGMGEDALKEMVFGLEKVQRHLEGKAVRKTIVVPNKLINIVCG"
     gene            2420..2938
                     /locus_tag="PKN85_01265"
     CDS             2420..2938
                     /locus_tag="PKN85_01265"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNT43062.1"
                     /translation="MNGKGLRRTGLVLVAAAMLMVTGACGYQLIGGKGIYGGDVSSIY
                     VPVFKNLTYEPHVSQPVTESFSRELLSTGLFTLSYEKADGILKGEITDVAMAPSSLNA
                     QGVVIEKSVSVTVKVSLSQKNGALIKEFAISDSEIYKIQEGAEEYNRREAIRRLSARM
                     ARRFSAQLLLEY"
     gene            complement(2946..3212)
                     /gene="rpsT"
                     /locus_tag="PKN85_01270"
     CDS             complement(2946..3212)
                     /gene="rpsT"
                     /locus_tag="PKN85_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNT43063.1"
                     /translation="MRKNKSAIKRAMQSEKRRLRNSHFKSTMKSRIKRAIGAIDRKEG
                     EVEAIFRDAVSSIDKAASKGVVHRNNAARKISRLARKLHKSQVA"
     gene            complement(3414..3698)
                     /locus_tag="PKN85_01275"
     CDS             complement(3414..3698)
                     /locus_tag="PKN85_01275"
                     /inference="COORDINATES: protein motif:HMM:NF038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HNT43064.1"
                     /translation="MVCTTLKPGIECLFMKKTGCSYSGGKCFTIVENCEGCTRVVEFE
                     TGKYCSSFPYPAQKWQKGSCPMSTHVKKAPKAEEQKINPLKASKRAAGKK"
     gene            3913..3985
                     /locus_tag="PKN85_01280"
     tRNA            3913..3985
                     /locus_tag="PKN85_01280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3946..3948,aa:Thr,seq:tgt)
     gene            3994..4078
                     /locus_tag="PKN85_01285"
     tRNA            3994..4078
                     /locus_tag="PKN85_01285"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4028..4030,aa:Tyr,seq:gta)
     gene            4146..4222
                     /locus_tag="PKN85_01290"
     tRNA            4146..4222
                     /locus_tag="PKN85_01290"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4180..4182,aa:Gly,seq:tcc)
     gene            4494..4569
                     /locus_tag="PKN85_01295"
     tRNA            4494..4569
                     /locus_tag="PKN85_01295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4527..4529,aa:Thr,seq:ggt)
     gene            4594..5793
                     /gene="tuf"
                     /locus_tag="PKN85_01300"
     CDS             4594..5793
                     /gene="tuf"
                     /locus_tag="PKN85_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNT43065.1"
                     /translation="MGKKKFERTKPHVNIGTIGHIDHGKTTLTSAITRCLSNKGQADW
                     IPFDQIDKAPEEKERGITIATAHVEYETDNRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVGANDGPMPQTREHILLARQVGVPCMVVYLNKTDMVDDPELIELVEMELRELLTQ
                     YEFPGDDIPIIKGSALKALEHGCGDPNCPDCKSIYELLAACDSYIPEPARDTDKPFLM
                     PVEDVFTISGRGTVVTGRIERGAIKPGEEVEIVGFRPTQKTVTTSVEMFRKILDQGMA
                     GDNVGLLLRGTKKDEVERGQVIAKPGSITPHTKFKGEVYILTKEEGGRHTPFFSGYRP
                     QFYLRTTDVTGVAKLPDGVEMVMPGDNVTLYVELVTPIALEKELRFAIREGGKTVGAG
                     VVTEIIE"
     gene            5806..6111
                     /gene="rpsJ"
                     /locus_tag="PKN85_01305"
     CDS             5806..6111
                     /gene="rpsJ"
                     /locus_tag="PKN85_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNT43066.1"
                     /translation="MRQRIRICLKAFDHRLLDQSVKEIVDAAKKTGAQVIGPVPLPTR
                     IQKFTVLRSPHIDKKSREQFEIRTHKRLMDIVDPTQQTVDALMKLELAAGVDVEIKS"
     gene            6122..6745
                     /gene="rplD"
                     /locus_tag="PKN85_01310"
     CDS             6122..6745
                     /gene="rplD"
                     /locus_tag="PKN85_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNT43067.1"
                     /translation="MAVTDILNIEGAKVGEIELKDEIFNCEVKEHLIHDVVKMQLARR
                     RRGTAKTKGRSEVSGGGRKPYKQKGTGQARRGSSRSPVMVGGGTIFGPQPRDYGYTVP
                     RKVRRSALRSALTVRNTASNMKVLDKLELANISTKTFFGIVKTLSLTKPLFIIDKKDE
                     TVERSARNIPYVKVLRVDGLNVYDIIRHEQLIMTVDAVKKVEEVLAS"
     gene            6742..7029
                     /locus_tag="PKN85_01315"
     CDS             6742..7029
                     /locus_tag="PKN85_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNT43068.1"
                     /translation="MNEYDIILRPIITEKSTLVKDTGNQYVFEVARSANKIEIRKAVE
                     KLFKVKVVDVHVSNMEGKKKRLGRFQGKRSDWKKAIVKLSPKDKITIFEGA"
     gene            7032..>7665
                     /gene="rplB"
                     /locus_tag="PKN85_01320"
     CDS             7032..>7665
                     /gene="rplB"
                     /locus_tag="PKN85_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNT43069.1"
                     /translation="MGVKEYKPTSAGRRFMSGLTFDEISKDKPEKSLLKPIKKTGGRN
                     HSGKITTRHIGGGHKRRLRIIDFKRNKFEILGKVAGIEYDPNRSANIALIHYIDGEKR
                     YILAPLGVKAGDTVIASMKPDTEIKEGNALPLKYIPLGTFVHNIEMKPGKGGQLARSA
                     GSYAQIVAKEGEMPLLYRNVSGKNNLDIGTSSASYVVVEGFEFVGGNGGVR"
BASE COUNT         1827 a         2088 c         2277 g         1473 t
ORIGIN      
        1 atccctcggg gaagatccac atgggacacg tgaggaacta ttccatcggc gacgtcatag
       61 cgcgatacat gacaatgaag ggactcaatg tcctccatcc catggggtgg gacgccttcg
      121 gcatgcccgc ggagaacgcc gccatcgaaa gggggataca accggcgacg tggacccacg
      181 aaaacatcga ttacatgaga gagcagttga agcgcctcgg tttcggctac gactggaggc
      241 gggaggtcgc cacctgcgac gtggactatt accggtggga acagtggatg tttctcaaga
      301 tgttcgagaa aggcctcgtc taccggaaga gctcgcccgt caatttctgt cccagatgcc
      361 agaccgtcct cgccaacgag caggtcgagg gcgggaactg ctggcgttgc ggcgaggagg
      421 tcatacagaa ggagctgacg cagtggttct tcgcgataac gaagtacgcc gacgagctcc
      481 acgaatactg tgacaagctg cccggatggc ccgagaaggt cacgagcatg cagaagaact
      541 ggatcggcaa gagttatggc gtcgaggtcc atttcaccct ggaggacgga tcgcccttca
      601 cgatcttcac gacccggccc gacaccctgt acggtgtgac cttcatggtc ctggcgcccg
      661 aacatccctt gaccctggaa ctgtccctgg ggacctccca ggaaaaggag gtggccgcct
      721 tcgtgcagaa ggtcaaggca caggacagga gcttccgggg ggagatcagc ctcgaaaaag
      781 agggtgtctt cacagggaaa tacgccataa accccctgac gggtcacagg atccccatat
      841 atgtggggaa cttcgtcctc atggagtacg gcacgggtgc catcatggcc gtccccgccc
      901 acgaccagag agacttcgag ttcgcgaagg agtatggtct tcccgtgatc gtcacgataa
      961 cgcccaaaga cagggagctc aacgcggcat ccatggactg cgcctacgtg gatgaaggcg
     1021 ttctcgtcaa ttccgacagg tttgacggta tgaacaaccg tgaggccatc ggcgccatca
     1081 tcgactatct cgaagagaaa ggtttcggga cgaggactgt caattaccgg ttgaaggact
     1141 ggggcatctc gagacagcgc tactgggggg ctcccatacc catcatctat tgtgatgact
     1201 gcgggaccgt cccggtgccc gagaaggacc tgccggtggt gttgcccgtc gacctcgaga
     1261 ttaggatggt gggccgctcg ccgctcgccg accacgcgcc gttctacgaa gtggagtgtc
     1321 ccgtgtgcaa gaaaaaggca cggcgggaga cggacacgat ggacacattc gtggagtcgt
     1381 catggtattt cctgcggtac gcctgcgccg attatcagga tggccctctc gacaggaagc
     1441 gcgtcgacta ttggatgccg gtggaccagt acatcggcgg catcgagcac gcggtcctgc
     1501 acctgctcta ctcacgcttc tttaaccgcg tcctcaacga actcggtctg gtcggtgtcc
     1561 gcgaaccctt caagaacctc ctcacccagg gcatggtcat caaggacggg gccaagatga
     1621 gcaaatccaa gggaaacgtc gttgacccca actatctcat cgagaagtac ggtgccgata
     1681 cggcgcgcct cttctgcctc ttcgccgcgc cgccggaacg ggacctcgac tggagcgacc
     1741 agggcgtgga agggagctat cgcttcctcc agagggtctg gaggctggct gccgagcacg
     1801 cgggttccct ggcggcagtg acagcggtcg accagatccc gggagcgggc ggtgatgaga
     1861 aacagctcat acacaagatc cacaggacca tcatgaaggt gacggacgac gtggagcggt
     1921 tccatctgaa taccgccatc gcgagcatca tggagcttgt caatatgacg tacaaattcg
     1981 tcgagaaggg aatggacggc gaaagaggga aggcgctgct caagggctcc attgaggcaa
     2041 tcctgagact cctctatccc ttcgtccctc acatcgtgtc cgagctgtgg cagaccatgg
     2101 gaggggatgt ggtccttctc aggtcgtcgt ggccgtcctg ggatgcggag ttggtgaaag
     2161 aggaaaaggt catgatcgcc gtgcaggtca acggaaagct ccgcgacacc tgtgaagccg
     2221 accggggcat gggggaggat gcgctcaagg agatggtctt cggcctggaa aaagtgcaga
     2281 ggcatctcga gggcaaggcc gtcaggaaga ccatagtggt gcccaataag ctgatcaata
     2341 tcgtgtgcgg atagaaaagg gcagggtgcc gggtcgtggg cgacgggcaa agagaggaat
     2401 gaaggtaatg gaatggacta tgaacggtaa aggcttgaga cggacaggac tggtcctcgt
     2461 cgcggcagcg atgctgatgg tgacaggggc gtgcggctat caactcatcg gtgggaaggg
     2521 catatacggc ggcgacgtgt cgtcgatcta cgtgccggtc ttcaagaacc tgacctacga
     2581 gccccacgtg tcccagcccg tcacggagtc tttttccagg gaacttctgt cgacgggact
     2641 cttcacgttg agctacgaaa aggccgacgg tatcttgaag ggcgagatca cggacgtcgc
     2701 gatggcaccg tcatccctga acgctcaggg cgtcgtcatt gagaaatccg taagtgtgac
     2761 ggtaaaggtc tccctttccc agaagaacgg ggccctcata aaagaattcg ccatcagtga
     2821 ttcagagatc tacaagatac aggaaggggc ggaggaatac aacaggcgcg aggccatccg
     2881 caggctctcg gcgcggatgg cgaggcggtt tagcgctcaa ttgctcctgg agtattgatg
     2941 aagccttagg ccacctgact cttgtgaagc ttccgggcca gacgggagat ctttctcgca
     3001 gcgttgttcc tgtgtacgac accctttgaa gccgccttgt cgatggagga aacggcatcc
     3061 cggaatatgg cctcgacctc tccttctttc ctgtcaattg cgccgatggc ccgttttatc
     3121 ctgcttttca tcgtcgattt gaagtgggaa ttcctgagcc tgcgtttctc ggactgcatt
     3181 gctcgcttga tggcggattt attcttcctc aaaggttgtc ctcctcttta agacttttct
     3241 ttatttatca tccatggacc cgaaagtcaa atagttctgc atcatttaaa aacgcgtccc
     3301 tttgtcggca tatgcacacg acaccgcaaa aacgacccgg cgatgcctgc gggatccttt
     3361 tcatcgaggc ggcttgtctt atgtggtcca gcgatcctaa acgtccgcgg ttcctacttc
     3421 ttgcctgcag ccctcttgga tgccttgagg ggattgatct tttgctcttc agccttgggc
     3481 gctttcttca cgtgggttga catcgggcac gagcctttct gccatttctg ggcaggataa
     3541 gggaaactcg aacagtactt tcccgtttcg aactcgacga ccctggtgca tccttcacaa
     3601 ttctccacga ttgtgaagca ttttcctcca ctgtacgaac atcccgtctt cttcatgaag
     3661 agacattcga ttcccggttt caacgttgtg cataccataa cggcatcacc tccttagaat
     3721 tgacaaaagc gtactatgta actacatttt ggtgctcatt gtcaatatgc tccctgaacc
     3781 cctcggactg aaatgtgatt ggcgaaggaa tctattgaca cagaggggca aaatatttta
     3841 attattattg ttttgttcgc gcggaacttc atgttgattt tggcatgttt cttgtagtat
     3901 aataactctt ttgctggtgt agctcaatcg gcagagcagc tgatttgtaa tcagcaggtt
     3961 gcgggttcga gtcccatcac cagcttataa aacggagagg ttcccgagcg gccaaagggg
     4021 gcagactgta aatctgttgg cgaagccttc ggtggttcga tcccacccct ctccaccatt
     4081 aaaaaatagg agcgttgaag tcgatcctcc accttgacgc tctggagcag atagtagcgt
     4141 gacgtgcggg agtagctcag ttggttagag catcagcctt ccaagctgag ggtcgcgggt
     4201 tcgagtcccg tttcccgctc caaaaaaccg aagcgggtga agactcccgc ttaagaggag
     4261 gcagcgggca accgtcgggc cggtaagaac ggtccggaag accagtgggg cctgcgggaa
     4321 agaagggcgg ccccaaaagg gcctgcctgg cagcatgacg gataatggag cccttggtgc
     4381 tccatggtaa cgtgcagata cagtcgttgc ccgctgaaaa acgggaagcg ggtagggagt
     4441 tgtttctgcg taaagatatt gcatggcgac tagggatatt atcggttata ggcgcccatg
     4501 tagctcagtc ggcagagcac atccttggta aggatgaggt cagcggttca atcccgctca
     4561 tgggctccat aaaaaatatc acggaggtcc tctatgggta agaaaaagtt tgaacggacg
     4621 aaaccacatg tgaacatagg aaccatcgga catatagatc acggaaagac caccctcacg
     4681 agcgccatca cgaggtgcct ttccaataaa ggtcaggccg actggatccc cttcgatcag
     4741 atcgacaagg cacccgagga gaaggagagg ggcatcacca tcgccaccgc ccacgtcgag
     4801 tacgagacgg acaaccgcca ctacgcccac gtggactgcc cgggccatgc cgactacatc
     4861 aagaacatga tcacgggagc cgcccagatg gacggcgcca tcctggtcgt gggagccaac
     4921 gacggcccca tgccccagac ccgtgagcac atactccttg cccgccaggt cggcgtcccc
     4981 tgcatggtcg tctacctcaa caagacggac atggtcgacg accccgagct catcgagctc
     5041 gtcgagatgg agctccgcga gctcctcacc cagtacgagt tccccggcga cgacatcccc
     5101 atcatcaagg gatccgcgct gaaggccctc gagcacggct gcggcgaccc gaactgcccg
     5161 gactgcaaga gcatctacga gcttctggct gcctgcgact cctatatccc cgagccggcc
     5221 cgtgacaccg acaagccctt cctgatgccc gtcgaggacg tcttcaccat ctccggccgc
     5281 ggcaccgtcg tgacgggcag gatcgaaaga ggcgccatca agcccggcga agaggtcgag
     5341 atcgtcggct tccgtcccac ccagaagacc gtcaccacct ccgtggagat gttccgcaag
     5401 atcctcgacc agggcatggc gggcgacaac gtcggcctcc tgctccgcgg cacgaagaag
     5461 gacgaggtgg agcgcggcca ggtcatcgcg aagcccggct ccatcacgcc ccacacgaag
     5521 ttcaagggcg aggtctacat cctgacgaag gaagagggcg ggcgccacac acccttcttc
     5581 tccggctacc gtccccagtt ctacctgagg accacggacg tgacgggcgt ggcgaagctg
     5641 cccgacggcg tcgagatggt catgcccggc gacaacgtga cgctctacgt cgagctcgtc
     5701 acccccatcg cgctggaaaa ggagctccgt ttcgccatcc gcgagggcgg caagacagtc
     5761 ggtgccggag tcgtcaccga gataatcgaa taaaggggaa ttacaatgcg gcagagaatc
     5821 aggatatgcc tgaaagcatt tgatcacaga ctgctcgatc agtccgtgaa agagattgtc
     5881 gatgcggcca agaagaccgg cgcgcaggtg atcggtcccg taccgctacc gacaaggatc
     5941 cagaagttca cagtcttgag atcgccgcac atcgataaga aatcacggga acagtttgag
     6001 atccgtacac ataaacggtt gatggacatc gtcgatccca cacagcaaac ggttgatgcc
     6061 cttatgaagc tcgaactggc ggccggcgtc gatgtggaga tcaaatctta ggagtcaggt
     6121 tatggcagtc acagacatac tgaacataga gggtgcaaag gttggagaga tcgagctgaa
     6181 ggacgagatc ttcaactgcg aggtgaaaga gcatcttatc catgacgtgg tgaagatgca
     6241 gcttgccagg cgcaggagag ggacagccaa gacgaaaggt cgcagcgagg tctccggcgg
     6301 tggccgcaag ccctacaagc agaaggggac agggcaggca cgccgcggtt cctcccgctc
     6361 tcccgtgatg gtgggcggcg gcaccatatt cgggccccag ccgagggatt acggctatac
     6421 ggtgccgaga aaggtcagga gaagcgccct gcgctcggcc cttacggtga ggaacacggc
     6481 atcgaacatg aaggtcctcg acaagctcga actggccaat atcagcacga agaccttttt
     6541 cgggatcgtc aagacactga gcctgacgaa accgctcttc atcatcgaca agaaggatga
     6601 gacggtggaa agatcggcca ggaacatccc ctacgtcaag gtgctcaggg tggacggact
     6661 caacgtctac gacataatcc gccatgaaca gctcatcatg accgttgacg cggtgaagaa
     6721 agttgaagag gtgctggcat catgaacgaa tacgatataa tcctgcggcc gatcattacc
     6781 gagaaaagca cgctggtgaa ggatacggga aaccagtacg tctttgaggt cgcgagaagc
     6841 gcaaacaaga tagagataag aaaggccgtc gagaagctct tcaaagtcaa ggttgtcgat
     6901 gtccatgtgt cgaacatgga gggaaagaag aagaggctcg gccggttcca gggcaagcgg
     6961 tcggactgga agaaggccat tgtgaagctc agtccgaagg acaaaatcac gatttttgag
     7021 ggtgcgtaaa catgggcgtg aaagagtaca aacctacatc cgcgggcagg cgtttcatga
     7081 gcgggctcac cttcgatgag atatcgaagg acaagccgga gaaatccctg ctgaaaccga
     7141 taaagaaaac gggcggacgg aaccacagcg ggaagatcac cacgaggcac atcggcgggg
     7201 gacacaagcg gcgcctgcgc ataattgatt tcaagcgcaa caagttcgag atcctgggga
     7261 aggtggccgg tatcgaatac gaccccaacc gctcggcgaa catcgcgctc atccattata
     7321 tcgacggcga gaagcgctac attctggccc cgcttggcgt gaaggccgga gacacggtga
     7381 tcgccagcat gaagcctgat acggagatca aggaaggcaa cgcccttccc ctgaaataca
     7441 tccccctggg cacctttgtg cacaacatcg agatgaaacc cggcaagggc gggcagttgg
     7501 cgcgaagcgc gggaagctac gcgcagatcg tggccaagga gggcgagatg ccgctcctgt
     7561 accgcaacgt cagcggtaag aataatcttg atatcggtac cagcagcgcg agctatgtcg
     7621 tagtcgaagg ctttgaattt gtcggcggca atggcggagt tcgca
//
LOCUS       DAOQTR010000028         7331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_11169_length_7331_cov_4.259208, whole genome shotgun sequence.
ACCESSION   DAOQTR010000028 DAOQTR010000000
VERSION     DAOQTR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11169_length_7331_cov_4.259208"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKN85_01325"
     CDS             complement(<1..683)
                     /locus_tag="PKN85_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43070.1"
                     /translation="MKKFLLALIAVALATPVLGNAGSVSSRYDVTFGGFVKYDFGYST
                     QNSHADPSYGYRSSTSDRAILADEYGNTYMSGAETRFNFLIKGPDLWGAKTSAFIEGD
                     FRGTTTGNQYGGFQLRHAWMKMKWNSSELLLGQAYQQWGMPYYAAQIGADDFKQYLKG
                     IRTPQVAFRHFFTKELSAMFGLTSATEWSGATRQYNDGYARSSWPGLQGEMAYWTDRC
                     GKIGSNNLK"
     gene            complement(989..1258)
                     /locus_tag="PKN85_01330"
     CDS             complement(989..1258)
                     /locus_tag="PKN85_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43071.1"
                     /translation="MKTGNVSQGSTLLGQTRSPRENRTARTKDSAPIEEKVDLSTKKA
                     PVIPSAATSYDDVLELLYGIDFSQFKNVEWISKDGSSKMMELLGA"
     gene            complement(1333..1602)
                     /locus_tag="PKN85_01335"
     CDS             complement(1333..1602)
                     /locus_tag="PKN85_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43072.1"
                     /translation="MGRKILDTTHLMTLPRTGQKSPFTPASAEHFADGQYLFVGLYEM
                     AVASFIDQAACEFQDYCNHLYCIDGRCDCPWSWKKSAGLALPASA"
     gene            1913..3064
                     /locus_tag="PKN85_01340"
     CDS             1913..3064
                     /locus_tag="PKN85_01340"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43073.1"
                     /translation="MIARKIQASVEESLKQFPVVGLIGPRQAGKTTLAKMILQKTETR
                     PLYLDLELSSDVNKLRDPEMYLRRYEDRLVIIDEVQRMPELFPLMRAMVDQKRSAGRF
                     LILGSASPALVRQASETLAGRAVYRELLPFSLDEIGCGNTDRLWLRGGYPLSYLAAGN
                     DESYSWREAFIRTYLEMDIPQLGIRVPALQLRRFWTMLAHSHGQLWNASKIAGSLGIS
                     APTAKHYLDILSDTFVVRQLQPFFANVKKRLIRSPKVYLRDAGLFHTLMRIKEKEDLD
                     AHPSAGASWEGFIIEQIIAAKPDDWEAYFYRTSAGAEIDLLLLDESGRPTAIEIKYSL
                     SPKPGRGFWSAIDDLACEKAFVIFPGDESYPLKENAFTLPVAQIQRIFH"
     gene            complement(3118..3193)
                     /locus_tag="PKN85_01345"
     tRNA            complement(3118..3193)
                     /locus_tag="PKN85_01345"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3158..3160),aa:Ala,seq:ggc)
     gene            3330..3992
                     /locus_tag="PKN85_01350"
     CDS             3330..3992
                     /locus_tag="PKN85_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNT43074.1"
                     /translation="MRSIVCLMAVLCLVLVSTAAVHGQGAAPGAGIRLPEPKYDGNVS
                     VEKALKERRSVRVYKDSPLSVAEVSQVLFAAQGITEPGRGLRTAPSARARYYLTVYLF
                     AGNVTGLKPGMYRYVPQGHSLALVAGGDAKGMLYKAAGQAPVDKAPAALVIAGSQARA
                     TNEGWMYLEAGHAAQNVYLQCVPLGLGTVVMAGFNADAVDKVIGLPAGERTLYIMPLG
                     RK"
     gene            4055..4519
                     /gene="sixA"
                     /locus_tag="PKN85_01355"
     CDS             4055..4519
                     /gene="sixA"
                     /locus_tag="PKN85_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNT43075.1"
                     /translation="MLLYLVRHGEAKREDEDPERGLTDNGMRHARRMGEFLAFMDVSL
                     DAVIHSTKKRAAQTAQILTESIRPASGIREENGLSPNDDPAPWVERISAMTADTAIVG
                     HLPFLDRLLGLLVLGDGGRTIIDFRPAGTVCLKPSGDGRWLIAWVLSPEVIW"
     gene            4516..5880
                     /gene="rmuC"
                     /locus_tag="PKN85_01360"
     CDS             4516..5880
                     /gene="rmuC"
                     /locus_tag="PKN85_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNT43076.1"
                     /translation="MSYLSLVIAFLAGVALGGVVFSLVVASRARARQAELEARAGSAE
                     AVVTELRQQFQQGRDEALRLQAELDGEREARVQAMTRCETLDLRLKEEMERFEAVRKE
                     LSETFKALSLDALASNTNEFRKYADEFMKLAEEKLKSQTAEGKKELEGKKELIDRNID
                     SIGKTLTEVQRRVEEVGKVSGERITEVTTLIKKHEEVTAKLRDTTEHLGRALASSKKR
                     GEWGERMAEDIIRLVGMAEGINYVKQTTLENTAGRPDFTFFLPNDLKVNMDVKFPLDN
                     FAHYLDAESDHDRKRYRDELLKNTKVMIKQVTTREYINPAENTVDYVIVFIPNEQVYS
                     FIQESDASIMDEALRQKVILCSPFTLYAVLAVVRQTIENVKLERTASEILRLLAEFSK
                     QWKLYKDKFKTMGDRIDAARKEYEVLVTTRTNMLERPLRKIDELSAADALELGTGELS
                     QDES"
     gene            5870..6322
                     /locus_tag="PKN85_01365"
     CDS             5870..6322
                     /locus_tag="PKN85_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43077.1"
                     /translation="MKASLSSLSRLVLVLLLSAPCLVAGADGGKDLPGFLEGNYGLVG
                     RRPGTGETYSGTVTIKARDGGLEVIRCVGGSRHAGQGSIVLVTADRIPALSVRWGDGK
                     KEYRGRYDIHGDADNYARLSGPYVAMEDEREFGWELLYVDPGNGPVCR"
     gene            6364..6567
                     /locus_tag="PKN85_01370"
     CDS             6364..6567
                     /locus_tag="PKN85_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43078.1"
                     /translation="MEDEDRLRTVLKQLIQNNEYLAENYRKWGRVAATGGLDAVSKEI
                     DEAGQFIQIATQKLRGALGLMGE"
     gene            complement(6554..>7331)
                     /locus_tag="PKN85_01375"
     CDS             complement(6554..>7331)
                     /locus_tag="PKN85_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNT43079.1"
                     /translation="GPMGIITLLTDFGVKDPYVGVMKGVILGIDPGARVIDMTHEVEP
                     QDMREAAFLIDDYWRHFPPGTVHVAVVDPTVGSARRPLIVSAKGHHFVGPDNGVFTLL
                     ADNVPDIHVIENRNFMLFPVSSTFHGRDVFSPAAARLSRGYSPQAFGRRITDPVLLSD
                     IHPVIEGRTLKGSVVRFDRFGNAITNIKGHALRYFAGEGPFEIRMGKHVFAAIGKTYC
                     EGGLACLEGSSGYLEFGVFKGDFRRETGTGKGDPVTVTLP"
BASE COUNT         1550 a         2065 c         2280 g         1436 t
ORIGIN      
        1 aacttgaggt tgttcgagcc gatcttgccg cagcggtccg tccagtatgc catctctccc
       61 tggagacccg gccaggagct tctggcatag ccgtcgttgt actgccttgt cgcgccggac
      121 cactccgttg cggaggtgag cccgaacatg gcgctcagct ctttggtgaa gaaatggcgg
      181 aaggccacct ggggtgtcct gatacccttg agatactgct tgaagtcgtc ggctcctatc
      241 tgggcggcgt agtatggcat accccactgc tggtaggctt gacctaagag cagttccgac
      301 gagttccact tcattttcat ccacgcgtgc ctgagctgga atccgccgta ctggttgccc
      361 gtcgtcgtgc cgcggaaatc gccctcgatg aaggcgctcg tcttcgcgcc ccacaggtcg
      421 gggcccttga taaggaaatt gaagcgggtc tcggcgccgc tcatgtaggt gttgccgtac
      481 tcgtcagcca ggatcgcgcg atcggacgtg ctgctcctgt acccgtagga agggtccgcg
      541 tgcgagttct gggtcgaata gccgaagtcg tatttcacga aaccgccgaa tgtaacgtca
      601 tacctgctcg atacgctgcc ggcattgcca agtaccggag ttgccagggc gaccgcgatc
      661 agggccaata agaacttctt cattcctgcc tccttagaaa tagttgtggt ggtacggaac
      721 acggtgctgc ctttccctcc tttccgtcaa tgtgggttta actgtcctga ggcaagatta
      781 accaaattta attaagcggg cgttaaatgc ctgttaaatt accgttaagc ggagagaacc
      841 cgaaggaggc cgtgaagggt tcttttcttg ccccgtgaag ggggacatga ggcaaatggg
      901 gggtggttgc gggcggccgg gatgccggga ggacgagagc gcgaagaacc tcggtcctcc
      961 gttcggtccc ggtcgatcgc agggtgtgtc aggccccgag gagctccatc atcttcgagg
     1021 aaccgtcctt ggagatccac tcgacgttct tgaactgact gaagtcgatg ccgtagagca
     1081 gctccagcac gtcatcgtat gaggttgcgg cgctgggaat gacgggtgcc ttcttcgtcg
     1141 agaggtccac cttttcctcg atgggcgcgc tgtccttcgt ccgggcggtc ctgttctcgc
     1201 ggggggaccg tgtctgtccg agcagggtgc tgccctgcga aacgtttcct gttttcattg
     1261 ccacctcctg aaaaacatag cgaaatacat tatatcacaa tagcggaatt gtaaagaaaa
     1321 ctttgtgata cctcatgcgg atgcaggcag ggcgaggcct gccgacttct tccaggacca
     1381 cgggcagtcg cacctgccat cgatgcagta gagatggttg cagtaatcct gaaactcgca
     1441 cgccgcttgg tcgataaagg atgcgacagc catctcgtag aggccgacga agaggtactg
     1501 gccgtcagcg aagtgttccg cggacgccgg ggtgaaagga ctcttttgcc ccgtcctcgg
     1561 aagggtcatc agatgtgttg tgtccaggat cttccgtccc atgtttcctc cgtacaccgc
     1621 tatagaatcg aatcaggaac cttgaaagca agaaccgtgc ctctgaccgg aaccagtgaa
     1681 ttcggatagt tagtgcgaat ggatttgtcg cctttggccc gaaagcggtc tttgttgacg
     1741 ccccggtgtc cgatatctga catgcccggt ctccggcagg cggcgttcct ttcgcgtaac
     1801 gtgttgtaac cgggagcggc gcgggaaggc acgacgatca gcatctcgtc gactgctatt
     1861 gcggaagaat attatccttg caaatcgaaa cctactcttt taatatacaa gaatgatagc
     1921 gagaaagata caggcttcag tcgaagagtc cctgaagcaa tttcccgttg tgggtcttat
     1981 cggtccccga caggccggaa aaaccactct ggccaaaatg atcctccaaa aaacggaaac
     2041 aaggcccctt taccttgatc tggagctttc ctccgacgtc aataagcttc gcgatcccga
     2101 aatgtacctg cgaagatacg aggacaggct ggtcatcata gacgaagtcc agcgcatgcc
     2161 cgagcttttc ccgctcatga gggcgatggt agatcaaaag agaagcgccg gccggttcct
     2221 tatccttggt tcggcatcac cagcccttgt aaggcaggcc tcggagacac tcgcgggccg
     2281 tgccgtatat cgcgagttgt tgcctttctc tcttgacgag atcgggtgcg ggaataccga
     2341 ccgactctgg cttcgtggcg gctatccctt gagttatctc gccgccggaa atgatgaaag
     2401 ctattcatgg cgcgaggcgt tcatcaggac ctatctcgaa atggacatac cccagctcgg
     2461 gataagggtg ccggcattgc agctcagacg attctggacc atgctggcgc atagtcacgg
     2521 acaattatgg aatgcatcaa agatcgcggg aagcctggga atttccgctc ccaccgcaaa
     2581 gcactacctt gacattctct cagacacctt tgtcgtgcgg cagctccagc cctttttcgc
     2641 gaacgtcaaa aaacggttga taagatctcc caaagtatac ttgcgtgatg ccggactgtt
     2701 ccacacgttg atgcgcatca aggaaaaaga ggaccttgac gcgcaccctt ccgccggggc
     2761 ctcctgggaa gggttcatta ttgaacagat cattgccgca aagccggacg actgggaggc
     2821 ttatttctat cggacctccg caggagcaga gatagatctt ttgcttcttg atgaaagcgg
     2881 caggcccacc gcgatcgaga tcaagtactc gttgagtcca aaacccggca ggggtttctg
     2941 gagcgcgatc gatgatcttg cctgcgagaa agcgtttgtg atatttcccg gcgatgaatc
     3001 atacccgcta aaggaaaatg cattcaccct gcccgtggcg cagatacaaa gaatatttca
     3061 ctgaaacgaa caccggtaac gaaaaagccg ctctttcgag cggctcatat ttttgaatgg
     3121 tggagctgaa ggggatcgaa ccctcgacct cttgactgcc agtcaagcac tctcccagct
     3181 gagctacagc cccgcaggta agtgttataa cagcatgagg atgaattgtc aatggctatt
     3241 gcgccgccca aggaggtgcc ttaagagttg caaaggtgcg gaaaacttat taccatgtga
     3301 gggtaacgat accgttcagg aggtgctcca tgaggtccat cgtttgtttg atggcggtcc
     3361 tttgtcttgt tctcgtgtcg acagcggcgg tgcacgggca gggcgcggca cccggtgccg
     3421 ggatcaggct gcccgagccg aaatacgacg ggaacgtttc cgtcgagaag gcgctcaagg
     3481 agaggcgatc ggtgagggtc tacaaggatt ctcccctgtc ggtggccgag gtctcccagg
     3541 tgctcttcgc ggcccagggt ataacggagc cgggaagggg cctgaggacg gcgccttccg
     3601 cgagggcccg gtactacctc accgtctatc tctttgcagg caacgtgacg ggacttaagc
     3661 cgggcatgta ccgctatgtg cctcaggggc attcgcttgc cctcgttgcc ggcggggacg
     3721 ccaaggggat gctttacaag gccgcgggcc aggcgccggt agacaaggcg ccggcggcgc
     3781 tcgtcatcgc cgggtcgcag gcaagggcga cgaatgaggg ctggatgtat ctcgaggcgg
     3841 gccatgccgc gcagaacgtc tatctccagt gcgtgccgct ggggctgggt acggtcgtca
     3901 tggcggggtt caacgccgac gcggtggaca aggtcatcgg tcttcccgcc ggcgaacgga
     3961 ccctttatat aatgcctctc ggcaggaaat agattatact gtattcccgg cggcgcggcc
     4021 tgtcggagac accacagaac aggaaaggac aacgatgctg ctctaccttg tgcgccacgg
     4081 cgaagcgaag cgcgaggacg aggaccccga gcggggcctc acggacaacg ggatgaggca
     4141 cgcccggagg atgggcgagt tccttgcctt catggacgtc agcctcgacg ctgtcatcca
     4201 cagcacgaag aagagggctg cccagacggc gcagatcctc acggaatcga tacggcccgc
     4261 ttccggcatc cgggaggaga atggcttgtc tcccaatgac gacccggcgc catgggtaga
     4321 aaggataagc gccatgaccg ccgacactgc catcgtcggt catctccctt tcctcgacag
     4381 gctcctcggc cttctcgtcc tcggcgacgg ggggaggacc atcatagact tcaggccggc
     4441 gggaacggtg tgcctcaagc cgtcgggtga cggaaggtgg ctcatcgcct gggtgcttag
     4501 ccccgaggtg atctggtgag ttatctctcc cttgtcatcg ccttcctcgc gggagtggcc
     4561 ctgggaggcg tcgtcttctc ccttgtcgtc gcctcccgcg cgcgcgcccg gcaggcggag
     4621 ctcgaggcga gggccgggtc cgcggaggcg gtcgtcaccg agttgaggca gcagttccag
     4681 cagggcaggg acgaagcact gcgcctccag gcggagcttg acggagagag agaggccagg
     4741 gtgcaggcca tgacgaggtg cgagaccctg gacctgcgcc tcaaggagga gatggagcgt
     4801 ttcgaggccg taaggaagga actctccgaa accttcaagg ccctttccct cgatgccctc
     4861 gcgtccaata cgaacgagtt caggaaatac gccgacgagt tcatgaagct cgccgaggag
     4921 aagctcaaat cccagacggc ggaagggaaa aaggaactgg aaggcaagaa ggagctcata
     4981 gacaggaaca tcgactccat cgggaagacc cttacggagg tccagaggcg cgtcgaagag
     5041 gtgggcaagg tgagcggcga gcgcatcacc gaagtgacca ccctgatcaa gaagcacgaa
     5101 gaggtcacgg cgaagctcag ggacacgacg gagcacctcg gccgagcgtt ggcaagctcg
     5161 aagaaaaggg gcgaatgggg cgagcggatg gccgaggata tcatccgtct cgtggggatg
     5221 gcggaaggta tcaactacgt caagcagacg acattggaga acacggcggg caggcccgat
     5281 ttcaccttct ttctccccaa tgacctcaag gtcaacatgg atgtgaagtt ccccctcgac
     5341 aacttcgccc actatctcga cgccgagagc gaccatgacc ggaagcggta ccgcgacgaa
     5401 ctcctcaaga acacgaaggt gatgataaag caggtgacga cccgggagta catcaacccc
     5461 gccgagaaca ccgtggacta cgtcatcgtc ttcattccca acgagcaggt ctacagcttc
     5521 atccaggaat cggacgcctc gatcatggac gaagcattga ggcagaaggt catcctctgc
     5581 tcgcccttca cgctctacgc ggtcctcgcc gtggtccgcc agaccatcga gaacgtgaag
     5641 ctcgaacgga cggcgtcgga gatactcaga ctccttgccg agttctcgaa gcagtggaaa
     5701 ctgtacaagg acaagttcaa gaccatgggc gaccgcatcg acgcggcgag gaaggaatac
     5761 gaggtcctcg tcacgacgcg gacgaacatg ctcgagaggc cgctgaggaa gatcgatgaa
     5821 ctgagcgcgg ccgatgccct ggaactgggg acgggggagc tctcgcagga tgaaagctag
     5881 tctgtcctca ttaagccgcc tcgtccttgt gcttctcctg tcggcgccgt gcctcgtcgc
     5941 cggcgctgat ggcggcaagg accttcccgg ttttctcgaa gggaactacg gtcttgtcgg
     6001 caggcgtccg ggcacgggcg agacctattc ggggacggtg acgataaagg cgagggatgg
     6061 cggcctcgag gtcatacggt gcgtcggcgg atcgcgccac gccggtcagg gttccatcgt
     6121 tctcgtgacg gccgacagga tacccgccct cagcgtgcgc tggggcgacg ggaagaagga
     6181 atacaggggc cgctacgaca tacacggaga tgccgacaac tacgccaggc tctcggggcc
     6241 ttatgtcgcc atggaagatg aacgggagtt tggctgggaa cttctctacg tcgatcccgg
     6301 gaacggcccg gtctgcaggt aagcgaggcc ctccgcggag cggccggatt aaggaggctt
     6361 gtgatggaag atgaggaccg tttacgtacc gtgctgaaac agctcatcca gaacaacgag
     6421 tatctcgccg agaattaccg gaaatggggc agggtcgccg cgacgggcgg tctcgatgcc
     6481 gtgtcgaagg agatcgacga ggccggtcag ttcatccaga tagccacgca gaagctccgg
     6541 ggtgccctcg gccttatggg agagtgaccg tcaccgggtc gccctttccc gtacccgttt
     6601 cgcgcctgaa gtcccccttg aaaacaccga attccaggta gccggagctg ccctcgaggc
     6661 aggcgaggcc accctcgcag taggtcttgc cgatcgccgc gaagacgtgt ttgcccatcc
     6721 gtatctcgaa agggccctcc ccggcgaagt accgcagggc gtggcccttt atgttcgtga
     6781 tggcgttgcc gaagcggtcg aagcgcacca cgctcccttt gagcgtccta ccctcgatca
     6841 cgggatggat gtcgctcaag agaacggggt ccgttatcct tcgcccgaac gcctgggggg
     6901 agtagccgcg ggacagcctg gcggcggcgg gggaaaagac atccctgccg tggaaggtgg
     6961 aactgacggg gaagagcatg aagttgcggt tctcgatgac gtggatatcg gggacgttgt
     7021 ccgccaggag ggtgaagacg ccgttgtcgg gcccgacgaa atggtgtccc ttcgccgaca
     7081 cgataagcgg ccgcctcgcg ctccccacgg tggggtcgac gacggcgaca tggaccgtcc
     7141 ccggcgggaa atgcctccag tagtcgtcga tgaggaaggc cgcctcgcgc atgtcctggg
     7201 gttccacctc gtgggtcatg tcgatgaccc gggccccggg gtcgatgccg aggatgaccc
     7261 ccttcatgac gcccacgtaa gggtctttga cgccgaaatc ggtgaggagg gttatgatgc
     7321 ccatcggccc t
//
LOCUS       DAOQTR010000029         7257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_11321_length_7257_cov_4.081089, whole genome shotgun sequence.
ACCESSION   DAOQTR010000029 DAOQTR010000000
VERSION     DAOQTR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11321_length_7257_cov_4.081089"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..523
                     /locus_tag="PKN85_01380"
     CDS             164..523
                     /locus_tag="PKN85_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43080.1"
                     /translation="MKRVRVLVVDDEENIRLLFKEELEEEDYDVETASNGMEALEKIK
                     GAPFDVVVLDIKMPVMDGIQTLNAIKNINKDQPVILCSAYGEFKQDLSSWVSDGYVVK
                     SADTRELKETIKEILSR"
     gene            618..692
                     /locus_tag="PKN85_01385"
     tRNA            618..692
                     /locus_tag="PKN85_01385"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:650..652,aa:Ala,seq:cgc)
     gene            820..2967
                     /locus_tag="PKN85_01390"
     CDS             820..2967
                     /locus_tag="PKN85_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43081.1"
                     /translation="MSLYTKILLFFITLVVLLSVVTTYSGIRSIERIAVNELEKGLAS
                     DIDLFSFSVDSEFYHMENRMRFVAMRRPLRDAIRRRDIGRIREIFTDLLQRESIDVLH
                     LVDDRNTPIVTLRSRRTDLPVTLPLPASGATTRGFTTIDTRDGVRPALFLSIPLEEPK
                     VVLSELVILDNANPLVRNLSDLLSRKRGEPVYVSLFKDDRRMFSTIFSLTGTATQDLP
                     EEITTALQGGRENYIGRTTIGRSGYYTVYKPSPYNEAGSPVWSYGIAVSEDIFMPFKK
                     RLLFVFVMISVLATSAVIIITFAITRGIDPSLRGILDICAKIEDGNTLSRIDSKRVTT
                     REFTQIASSINRMLDSISEREKTISDNMDRIRAINAELEEKTNIIRQDRMRFLSILET
                     VDEGVVALDHEGVIIYYNRAAAAITGTDPEAALGDTWKSLFPSLDISGEDHAALQELT
                     IPREDSSEPLYLRVLASPWSVENAPQGRILVLQDISREKRMEEFKADFVSSINHDIRS
                     FLVPVSGFIGRILEGKYGPLEDAVRERLLNVADNVSKVHHLVENYLNVSRIESGKLDL
                     AIGPVDMNEVIRDVAKLYAPRVIFADGATPQALVLADKDYIERVITNLVVNALKFSPE
                     DSTVRIGTRVEGGMLVTFVEDRGAGIPARELRFIFEKYRRGSLGRREEGSGLGLFIVK
                     SVVEGHGGSIWVDSVPGRGSTFFFSLPLSRGEI"
     gene            3031..4293
                     /locus_tag="PKN85_01395"
     CDS             3031..4293
                     /locus_tag="PKN85_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43082.1"
                     /translation="MRKIVIVLLAVVLASCSYMPWGKKKDEDPSKTTKKTDSAAQKKP
                     ARGEPEEMKEPELGDIKVIDGVEYIYTRNRKYMLTPYEPPYVWVRKDQYAPGIGEALS
                     FGIGGSGPSKKERDELERRIAKLEEELKKKGINPQMAYPTQMGYIPVGLGYMPGLISF
                     EYPSPKMKRRIVVLPVTDNTNYKSEGLGELATKRLISRLESTGTVICVDPNTLNVRGS
                     YFDAVNAKKMNDDFGVQAILKTALSDVYTSSSRIEGKDEKETSFAMSKLAIDVFNTET
                     GKIMKQLSGRNPISLSREKGDLSTEKAKIKAIDLAIEMIAEDVLKTVLSIDWHSRVAS
                     VDGTKVYINAGRLSGLEKGRVLEVYAPGEEVIDPRTKQPLGRKKGAYKGELEVVELFG
                     VDASWAKTNKGGSFSSTDLVYVKQAAVK"
     gene            complement(4382..5938)
                     /locus_tag="PKN85_01400"
     CDS             complement(4382..5938)
                     /locus_tag="PKN85_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43083.1"
                     /translation="MKDTSKTTEGMSAANGKTQGKRDNGGYAPEFRDLYHSFFEFAPE
                     AMGVTDPETGLILDVNKAFEAWSGYTREELLGKSTVELGFWVHEEDRRAIIQRLCETE
                     YVEGIELDWKNAKGDVKRLLFSARLIDTPRGKLVLSIGKDITERKNMERVLRESERRL
                     SDIIEFLPDATFVIDREGIVIAWNRAMEEMSGVSKKDMIGKSDHVYAIPFYGRRRKLL
                     IDMVGEVDENDDSKYIYVKRNGHTIYAEAFTEALYGGKGACLWGTAAALLDEHGNRVG
                     AIESIRDITEHSMAEEKLRSSYEELRRLSAHIEEVRENERAAISRELHDVLGQILAVI
                     NMDLRWLNKKIPAHEKELLGKVAAALTLVKQATRTIQKVSSGLRPVVLDDFGLAAAVD
                     HALKEFRDQTGITTSLIISGLVTDVGKDTSIALYRIFQEALTNVMRHSGATRVEVSLQ
                     VEGDDIQLMVRDNGRGISKQQISDWNSLGILGMRERVSFIGGRIDITGSSGAGTCITV
                     DLPLKRTEAK"
     gene            complement(6051..6596)
                     /locus_tag="PKN85_01405"
     CDS             complement(6051..6596)
                     /locus_tag="PKN85_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNT43084.1"
                     /translation="MSDQGLIDRSDCLLVVIDVQEKLMPAISGKERVIANTATLVRFC
                     GICGIPVIFTEQKKLGPTVPEVKDLTQGFAAVEKVHFNCFLNGEFREHVDRLGRKTIV
                     LTGAESHICVAQTAIAALPRLNVHIIADAVSSRLPDNRAIALDRMRDAGAVISSTEMF
                     IYEILREAGTEEFKAVLPLVK"
     gene            complement(6730..6981)
                     /locus_tag="PKN85_01410"
     CDS             complement(6730..6981)
                     /locus_tag="PKN85_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43085.1"
                     /translation="MPWRSFTQNLVKFLNENGSIFLAAVDNGKFGVRPFQLMMEEGGG
                     LYFCTNNTRDNPIFEVFFIANWAATIADFSGNLPEMVAF"
BASE COUNT         1582 a         2126 c         2040 g         1509 t
ORIGIN      
        1 tcttcacgac gaaggagcgc ggcaccggcc tcggccttgc catttccaac aagatcgtta
       61 caaaccataa ggggcacata gaggtggaga acgtggctgg aaagggagtg agcttcatcg
      121 tctatctccc tatgaaaaat aacgtgctgc aagaggagtt cgcatgaaaa gagtacgggt
      181 gctcgtcgta gacgacgagg aaaatatcag gctgctattc aaagaggaac tggaggaaga
      241 ggattacgac gtggaaaccg cgtccaacgg catggaggcc ctggagaaga tcaagggcgc
      301 tcccttcgat gtggtggtcc tggatatcaa aatgcccgtt atggacggga ttcagacgtt
      361 gaatgccata aagaacatca acaaggacca gcccgtcatc ctttgctcgg cctatggtga
      421 gttcaagcag gacctgtcga gctgggtttc cgacggatac gtcgtcaaat cggcagacac
      481 gcgggaactc aaagagacga tcaaggaaat actgagccgg tgagcctttc ctgcctgagc
      541 ccatggcggc ccgcccgcgg ccgtccccag aaaacccttg atatgaggga tgatatggac
      601 tatgattatg cctacccggg ggtgtagctc agcgggagag cacctcgttc gcaacgaggg
      661 ggccacgggt tcaatccccg tcacctccac cattcttgca tcatctgccg ggtcagattc
      721 gccctcccta ccgggtccgc tcccacccga cgagccgccg cgcgtttccc gtcttttccc
      781 tggaaccttt gaacctttga accctggaac cagtttttaa tgagcctcta caccaagata
      841 ctcctcttct tcatcaccct tgtggtcctt ctttccgtgg tcacaaccta ttcagggata
      901 cgctccatcg aacggatagc cgttaacgag ctggaaaaag gcctcgcctc ggacatcgac
      961 ctcttttcct tttccgtcga cagcgagttc taccacatgg aaaaccggat gaggtttgtt
     1021 gccatgcgca gaccgctgag ggacgccatt cggcgacggg acatcggcag gatccgcgag
     1081 atcttcaccg acctgcttca gcgggagagc attgacgtgc ttcacctcgt ggacgacagg
     1141 aacacgccca tcgtcaccct gcgaagccgg cgtacggatc ttcccgttac gctgccgctg
     1201 cccgcctccg gtgcgaccac caggggtttc accaccatcg acaccaggga cggagtccgt
     1261 ccggccctct ttctttccat acccctcgag gagccgaagg tcgtcctgag cgagcttgtc
     1321 atcctcgaca acgcgaatcc tctcgtcagg aacctgagcg atctgctctc ccgcaagcgc
     1381 ggggagcccg tgtatgtctc acttttcaag gacgacagga ggatgttcag caccatattc
     1441 tcgctgaccg gcaccgctac ccaggacctt cccgaagaga tcacgaccgc tcttcagggc
     1501 ggccgggaga actacatagg cagaacgacc ataggaagat ccggctacta caccgtctac
     1561 aagccgtcgc cgtacaacga ggcggggtcg ccggtgtggt cctacggaat cgccgtgagc
     1621 gaggatatct tcatgccctt caagaaacgc ctcctcttcg tctttgtcat gatatccgtt
     1681 ctcgcgacgt cggcggtcat catcataacc ttcgcgatca cgcggggcat cgatccctcc
     1741 cttcggggca tcctggatat atgcgcgaag atagaggacg gaaacaccct ttcccgcatc
     1801 gacagcaaga gggtgacgac ccgcgagttc acccagatcg cttcgtccat caacaggatg
     1861 ctcgattcca tctccgaacg ggagaagacc ataagcgaca acatggacag gatacgggcg
     1921 atcaacgccg agcttgagga aaaaacgaac ataatccggc aggaccggat gcggttcctg
     1981 tccatcctgg agaccgtgga cgaaggggtt gtcgcccttg accatgaagg cgtcatcatc
     2041 tattacaaca gggccgcggc ggccatcacg ggaaccgacc cggaggccgc cctgggcgac
     2101 acctggaaaa gcctctttcc gtcactcgac atctccggcg aggaccacgc ggccctgcag
     2161 gaactcacca tcccgcggga ggactcctcc gaacccctgt atctcagggt ccttgcttcg
     2221 ccctggtccg tggagaacgc tccccagggc cggatcctgg tgcttcagga tatatcgagg
     2281 gagaagagga tggaggagtt caaggcggat ttcgtttcct cgataaacca cgacatacgg
     2341 tcctttctcg ttcccgtttc aggtttcata ggcaggatcc ttgagggcaa gtacggccct
     2401 ctggaagacg ccgtgaggga aaggctcctg aatgtcgcgg acaacgtgtc gaaggttcac
     2461 caccttgtcg aaaactacct gaacgtgtcc aggatcgaat cgggaaagct cgacctcgcc
     2521 atcggccccg tcgacatgaa cgaggtcatt cgcgacgtgg cgaagctcta tgcgcccaga
     2581 gtgatcttcg ccgacggcgc aaccccgcag gccctcgttc ttgcggacaa ggactacatc
     2641 gaacgggtca tcacgaacct tgtcgtcaac gccctgaagt tctcgcccga agattccacg
     2701 gtcaggatag ggacccgcgt cgagggcggc atgctcgtga ccttcgttga ggacaggggc
     2761 gcggggatac ccgcacggga acttcgcttc atcttcgaga agtaccgccg cggaagcctg
     2821 gggagacggg aagagggcag cggactgggc ctgttcatcg tgaaatccgt cgtggaagga
     2881 cacggagggt ccatctgggt cgatagcgtc cccggcaggg gaagcacctt cttcttttcc
     2941 cttcccctct ccaggggcga gatataaggt ttgacaaagc tcaacattta aaaatttgac
     3001 ttttcgcagt tacatattta taatcactcg atgcgtaaaa tcgtaattgt tttactggca
     3061 gtcgttctcg cttcctgcag ctacatgccc tggggtaaaa agaaagacga ggacccctcc
     3121 aagactacga agaagaccga ttcggccgcc cagaaaaaac ctgctcgagg cgagccggag
     3181 gaaatgaaag agcccgaact gggcgacatc aaggtcatcg acggcgtgga atacatctac
     3241 acgcggaaca ggaaatacat gctgacgccc tacgagccgc cctacgtctg ggtccgcaag
     3301 gaccagtacg ccccgggcat cggggaggcg ctttccttcg gcatcggcgg cagcggtccc
     3361 agcaagaagg agcgggacga actggaaaga aggatagcga agcttgaaga ggaattgaag
     3421 aagaaaggga ttaatcccca aatggcctac ccgacgcaga tggggtatat ccccgtcggt
     3481 ctggggtaca tgcccggtct catttctttc gagtatccgt cgcccaagat gaaacggcgc
     3541 atcgtggtcc tccccgttac ggataatacc aactacaagt cggaaggctt gggagagctt
     3601 gccacaaagc ggctcatatc ccgcctcgag agcacgggga cggttatatg cgtcgacccc
     3661 aacacgctca atgtcagggg atcctacttc gatgccgtca acgcgaagaa gatgaacgac
     3721 gatttcggcg ttcaggcgat cctcaagacc gccctttccg atgtctatac gagttcatcg
     3781 aggatcgagg gcaaggatga gaaagaaaca tcctttgcta tgtcaaaact ggccatcgac
     3841 gttttcaaca cggagacagg caagataatg aagcagcttt ccggcaggaa ccccatctcc
     3901 ctgtcccgcg aaaagggcga tctcagcacg gaaaaggcaa agatcaaggc gatcgacctt
     3961 gccatagaga tgatcgcgga agatgtcctg aagaccgtcc tctccatcga ttggcattcg
     4021 cgtgtcgcct ccgttgacgg gacaaaggtc tatatcaacg cgggacggct gtccggcttg
     4081 gaaaagggca gggtcctcga ggtctacgca cccggcgagg aggtcatcga ccctaggacg
     4141 aagcagccgc tcggcaggaa gaaaggcgcc tacaagggag agctggaggt cgtggagctc
     4201 tttggtgttg acgcaagttg ggccaagacc aacaagggcg gcagtttttc ctccaccgac
     4261 ctcgtctacg tgaaacaagc agcggtaaaa taagatcaaa aacaggtaac ggggttacaa
     4321 attcccgatg atgggtcacc ggattggtgc gtccggcgac cgtatgcgaa gattctgtct
     4381 ctcatttcgc ctccgttctt ttcaggggga ggtcgacggt gatgcaggtc ccggccccgc
     4441 tcgatcccgt gatatcgatc cttccgccga tgaaagagac gcgttcacgc attcccagta
     4501 tgccaaggga attccagtcg gatatctgct gcttcgagat accgcggccg ttgtccctga
     4561 ccatcagttg aatgtcatct ccctcgacct gaagcgagac ctccacgcgt gtcgctccgg
     4621 aatggcgcat cacgttcgtc agggcctctt gaaatatccg gtagagtgct atgctcgtgt
     4681 ccttgccgac atccgtaacg aggccgctga taatgagact ggtggttata cccgtctggt
     4741 cgcggaactc cttcagggca tggtcgacgg cggcggcgag accgaagtcg tcgaggacga
     4801 cgggcctcaa gcccgatgat accttttgaa tggtccttgt tgcctgtttg acgagggtaa
     4861 gagcggcggc aaccttgccg agcagttcct tttcgtgcgc cggtatcttc ttgttgagcc
     4921 agcgcaggtc catgtttatg accgcgagga tctgccccag aacgtcgtgc aactcccggg
     4981 agatggcagc ccgttcgttc tcccgcacct cctcgatgtg cgcggagagc cttcgcagct
     5041 cttcgtagga tgacctcagc ttctcttcgg ccatgctgtg ctccgttatg tcccggatcg
     5101 actctatcgc gccgaccctg ttgccgtgct cgtcgagcag cgcggccgcc gttccccaga
     5161 ggcaggcccc cttgccccca tagagggcct cggtgaacgc ctcggcgtag atcgtgtggc
     5221 cgttcctctt gacgtatatg tatttggagt cgtcgttctc gtcgacttct ccgaccatgt
     5281 ctatgagaag cttcctgcgc cgcccgtaga aggggatggc gtagacgtgg tccgacttcc
     5341 cgatcatgtc cttcttgctc accccgctca tctcctccat ggcgcgattc caggcgatca
     5401 cgatcccttc cctgtctatg acgaaggtgg cgtcgggaag gaactcgatt atgtccgaga
     5461 gtcttcgctc cgactccctg aggacgcgtt ccatgttctt gcgctcggtg atgtcctttc
     5521 cgatggacag aacgagctta ccccgtggtg tatcgatgag cctggcggaa aagaggagcc
     5581 tctttacgtc gcccttcgcg tttttccagt caagctcgat accttcgaca tactccgtct
     5641 cgcacagcct ttgtatgatc gcccgcctgt cctcctcgtg gacccagaaa ccaagctcga
     5701 cggtactctt ccccagaagc tcctcgcggg tgtaaccgga ccatgcctcg aaggccttgt
     5761 tgacatcgag gatcagaccg gtttcgggat cggtcactcc catggcttca ggagcgaact
     5821 cgaagaagga gtggtagagg tcacggaatt cgggcgcata cccgccgttg tcccgtttcc
     5881 cctgtgtctt tccatttgca gcggacatac cttccgtggt cttcgaggtg tccttcactg
     5941 tgcctccgcc tcaggtaaaa tgggctccct ttcatggaca tcgagagacc gcccgagaac
     6001 ccgtcgcttc accggattat cgcatgaatt tcaggcaagg tttagaaaaa tcacttcaca
     6061 aggggcaaga cggccttgaa ctcttcagtg ccggcctcac gaagtatctc gtagatgaac
     6121 atctccgtgg aacttatgac ggcaccggcg tcacgcatcc tgtcgagggc gatcgccctg
     6181 ttgtcgggca acctggaaga cacggcatcg gcgataatgt ggacgttgag gcggggcagg
     6241 gcggcgatgg cggtctgcgc gacacatatg tgggactcgg ccccggtaag gacgatggtc
     6301 tttcggccga gcctgtccac atgctcgcgg aactcgccgt taaggaagca attgaaatgg
     6361 accttctcca cggcggcgaa gccctgcgtg aggtctttga cctcggggac cgtcggcccc
     6421 agcttctttt gctccgtgaa gatcacgggg atcccgcata tgccgcagaa cctcaccagc
     6481 gtcgcagtat tcgcgatcac cctttccttc ccggagatcg ccggcatcag cttttcctgc
     6541 acgtcgatga ccacgagaag acagtcgctc ctgtcgatga ggccctgatc agacatcgct
     6601 tcctcctttt atccccgaac cctcggccgg cctcatggtc cacgccgtcg ccacagtata
     6661 aaccagttcg aaggcggggg taaagagggt ttcaggtccc gccgagaggg aagggccttc
     6721 ctcttccggt cagaaggcca ccatctccgg gaggtttccg gaaaagtccg cgatcgttgc
     6781 ggcccagttt gcgatgaaaa agacctcgaa gatggggttg tcccgggtgt tgttggtgca
     6841 aaaatacaac ccgccccctt cctccatcat aagctggaaa ggtctgaccc caaacttgcc
     6901 gttgtcgacg gcggctagaa aaatgctgcc gttctcgttc agaaacttca ccagattctg
     6961 cgtgaatgac ctccatggca tttttcgtgc ctccacgact gttcccctgc gtcaccgggg
     7021 acaccggaga gattttggta ttgaccgata cacgccttat agtttactct acaaataaca
     7081 taaggtttac actatggcgg gaagaacgag gaagagaggc gaggcggttc gggccttcat
     7141 cttgagaaat gtggaggatc ggtcgagaga cgttgtctcg aggacgacgg ggcaattcgg
     7201 catcagccgc caggcggtcc acaaccccgc gatgccggtg cccacgatga tgggccg
//
LOCUS       DAOQTR010000030         7128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_11603_length_7128_cov_3.731231, whole genome shotgun sequence.
ACCESSION   DAOQTR010000030 DAOQTR010000000
VERSION     DAOQTR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11603_length_7128_cov_3.731231"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKN85_01415"
     CDS             complement(<1..220)
                     /locus_tag="PKN85_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43086.1"
                     /translation="MKKFPLRRWCGVSGTAGFDIPALSCDIILPMTDPDFLPVTAAVI
                     EKDGKILIARRKHPYPFPGPPWEFPGGKL"
     gene            436..678
                     /locus_tag="PKN85_01420"
     CDS             436..678
                     /locus_tag="PKN85_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43087.1"
                     /translation="MNELVQARGNPDRYETDGKGNRVLVYEWTWESTYETPGRAWRDA
                     SGLRWTNPKTETILHREKRAYYVNKQGIVFDGKWAW"
     gene            complement(800..2050)
                     /locus_tag="PKN85_01425"
     CDS             complement(800..2050)
                     /locus_tag="PKN85_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT43088.1"
                     /translation="MGKSVSRRSGIFYGWIIVAIAAVGTCTGFGTIYSFSVFFKAWLG
                     EFGSSRAVLSGIYSVAFIIYGIASMVMGWFSDRYGPRKVIATGGSIMAVGCMLTAFCH
                     HTAVLYLTWGLTVGIGVGTCYAPTAATVSKWFIHRKGMAMGILVCGLGMGTLIFSPLS
                     EHLIKSFGWRSAIFILGVIALTVYIAEALIIRGSPRDVGLEPLTAPMKNGGDADPDAA
                     RKLDMPEHSMTVAEALKTRNMWFIFVIHGLWMVGVGIIMAHFVPYATDQGTNVNRAAA
                     MMGSVGAMSVIGRFSLGILTERWGISAPSIIILCCQCLTMLLPFLGSSQWQLWAFVVI
                     FGFGYGGLASIFPLAHAELFGVKAMGSLFGISLLGATIGGSAGPLIAGFSFDMTRSYF
                     TAFGAGAISIAIAILLATGIKKKA"
     gene            2301..2576
                     /locus_tag="PKN85_01430"
     CDS             2301..2576
                     /locus_tag="PKN85_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43089.1"
                     /translation="MGEGMGVLRGEVVLGGLEDDYRTYDDRGDEVDNSFRIKNTIFFL
                     SRRHGGTRRPPDLGRLQEPAAVFSLFPPLSASSVSLREERKRRTGER"
     gene            complement(2588..3817)
                     /locus_tag="PKN85_01435"
     CDS             complement(2588..3817)
                     /locus_tag="PKN85_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HNT43090.1"
                     /translation="MPFIADLHMHSHYSIATSRDMTPEGLWKWAQLKGVTVIGTGDAI
                     HPGWFRELARKLDPAGNGLFALRADMREDIIPRSCVAEVFFLPSVEISCIFTRNGKTR
                     KAHCIVLFDDMESALRLQNRLSKVGNISSDGRPILGLDAKRLLAMVIDDCPRALFIPA
                     HVWTPHFSVLGSASGFDSLEECFEELTPRVHAVETGLSSDPPMNWRVSPLDRLTLVSN
                     SDAHSPQKIGREANILNTDLSFEGIARAVTTREGFDGTIEFFPEEGKYHYDGHRPCKV
                     RMTPRQAADAEYRCPVCGKKLTLGVLNRVEALADREAGFRPKAAKPFTSLIPLREIIA
                     DTLRAGAQTKKVDKVYFDLLEKLGPELAILMDAPLTDITRTGHPDIADAIARVRAGDV
                     TIEPGYDGEYGRVAIRR"
     gene            complement(3937..4407)
                     /locus_tag="PKN85_01440"
     CDS             complement(3937..4407)
                     /locus_tag="PKN85_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNT43091.1"
                     /translation="MRATKKEIRDPAVLAGLLRHCPVGRLGTVGADGSPMIKPLSFVY
                     LDGDIYFHSAREGEKMDDVKRDARVCFEIDLPAGYVRSDASPCRAGYRYQSVIVKGRA
                     LVVEDRETRLRALRALMEKYQPEGGYGPFPEDKLALTAVVRIAIDHISGKQDLR"
     gene            4816..6348
                     /locus_tag="PKN85_01445"
     CDS             4816..6348
                     /locus_tag="PKN85_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HNT43092.1"
                     /translation="MSKDEKKEEKVSGISRRNFITGTGVAMAGALATGALLPGKVEAV
                     PPPKKWGRTTDVLIIGTGYSGLAAAIEAHDAGCKVAIIEKAVVIGGNSAIASGIYNCS
                     EPDVQAKYGIKDSPEQHYQQTLAAGDFRGDPEKVKYMTYHALEGRKWLEKQGVQFDEK
                     PYTAVGALWARSFDPVKKGRGGSIIRALKAQVDARKIPVMMNVKLKALVRQKPLEGDI
                     MGAVVTEKGKDVYFKAQKAVILATGGFAADVAMRSKHDPRLTAEVPTTNVPWATGEAI
                     VIAENEGADVHGMDYIQMLIACNYFTKKYGSLTNLGVDHALFVNLDGKRFVAEDQRRD
                     VMAEAVLKQKNKVLLWVADEKCSKRFNPKMTEEIIKDGLAFRANTLEELAKILNAKLE
                     VPEQAFIESVAKYNESVTKGKDEEFGKKPQNLKPVTTAPFYASPTQAGVHHTMGGIQT
                     KGTTCQVIDRENKIVPRLYAAGEVTGGVHGANRVGGNATVDCIVFGRNAGINAAKEKS
                     WS"
     gene            complement(6453..>7128)
                     /locus_tag="PKN85_01450"
     CDS             complement(6453..>7128)
                     /locus_tag="PKN85_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43093.1"
                     /translation="VIVGGCTDANGSHGCYSSGGAWVLLDYPDATSTEAFGVNNHGII
                     VGQYTGEDGSVHGFSYDGTIWNTLDHPGATSTIAYGINDRGTIVGTFEDANGTHGFLA
                     KAVSDLTSLAIDIRPWSAENVLHLLERGLLPVAVLGGNAFDALGTIDLGTISFGRTGD
                     EDSMAFCSPWDWDVNADGYQDLICLFWVQKAGFQCGDTGGILKGMTQDGTTLEGRDAV
                     KVIPCR"
BASE COUNT         1545 a         1983 c         2291 g         1309 t
ORIGIN      
        1 cgagcttgcc gccggggaac tcccagggag gccccgggaa gggatagggg tgtttccggc
       61 gggctatgag tatcttgccg tccttctcaa taacggcggc ggtgacaggc aggaaatcgg
      121 ggtcggtcat cggaagaata atatcgcacg agagcgccgg gatgtcaaaa cctgccgtgc
      181 ctgatacgcc acaccaccgc cgcaaaggga actttttcat ccttaccccg tcatattggg
      241 gagaaaggag ataacatgat agcgacgcaa atacccttgg ccccttcacc tatggtcgaa
      301 ccaatgacaa agtgcgtctc gtgctggcta tggcggttct ttatcgttgc agccttgggt
      361 tacctgttcc ttacgctggg aggttgcggg gttctaagca ggaacgccga gataagtgcc
      421 tggatcggac gccccatgaa cgaacttgtt caggcccgcg gcaacccgga ccggtacgag
      481 accgacggca aaggcaacag ggtcctcgtc tatgaatgga catgggaatc cacctatgag
      541 acccccggcc gcgcctggcg cgacgcaagc ggtctgcgct ggactaatcc caaaacggag
      601 accatcctcc acagggaaaa aagagcgtac tacgtaaaca agcagggcat cgtcttcgac
      661 gggaagtggg cgtggtaaag ccagcttgag tggcttgaat agcttgaata gctcgaaggc
      721 caagaaataa agagaccttt ccttctctct tcagaggatg cgcctttgac cctgaagcca
      781 ctcaagcgat tcaagctgtt caagccttct tctttatccc cgtggcgagg aggatggcta
      841 tggctatgga gatcgccccg gcgccgaagg ccgtgaagta ggaacgggtc atgtcgaagg
      901 agaaaccggc gatgaggggg ccggccgagc cgcctatggt ggcgcccagg agcgagatgc
      961 cgaagaggga gcccatggcc ttcaccccga agagctcggc gtgggcaagg gggaagatgg
     1021 aggcgaggcc gccgtacccg aacccgaaga tgacgacgaa tgcccagagc tgccactggg
     1081 agctccccag gaagggaagg agcattgtga ggcactggca gcagaggatt atgattgaag
     1141 gagcgcttat cccccaccgc tcggtgagga tcccgaggga gaacctgccg atgacgctca
     1201 tcgcgccgac ggaccccatc atggctgccg ccctgttcac attcgttccc tggtcagtgg
     1261 cgtagggcac gaaatgggcc atgatgatcc cgacaccgac catccacagg ccgtgtatga
     1321 cgaaaatgaa ccacatgttc ctcgtcttca gggcctccgc cacggtcatg gaatgctccg
     1381 gcatgtcgag cttgcgggcg gcgtcagggt ccgcgtcccc gccatttttc atgggagcgg
     1441 taagaggttc gagtcctaca tcccgggggc tcccacggat gatcagggcc tccgctatgt
     1501 aaacggtcag tgcgatcacg ccgaggatga agatggcgct ccgccacccg aatgacttga
     1561 taagatgttc cgataggggg gagaagatca gggtgcccat accgaggccg cagacgagga
     1621 tacccatggc catccccttg cggtggataa accacttgga aacagttgcg gcggtggggg
     1681 cgtagcaggt gccgacaccg atgccgaccg tgaggcccca ggtcaggtag aggacggccg
     1741 tgtgatggca aaaggccgta agcatgcagc ccaccgccat gatggagccc cctgtggcga
     1801 tgaccttccg ggggccgtac ctgtcagaga accatcccat gaccatggag gcgatgccgt
     1861 agatgatgaa ggcgacggaa tatatgccgg aaaggacggc gcgagagctt ccgaactctc
     1921 ccaaccaggc cttgaagaag acgctgaagg aatagatcgt accaaagccc gtgcaggttc
     1981 ccacagccgc gatggcgacg atgatccagc catagaatat accgctccga cgagaaacgc
     2041 tctttcccat gccagctcca acaagaaatc aacgctcgca ctgtccccga agcgactggc
     2101 cttccttccc caccatgcca gttctgttac cataacacag acccggcgcg ggggggaaga
     2161 aaaagatggg ggaaaaccaa agacgttgcc tctcgcccgc ttcgctcgag ggcacaaagt
     2221 tagtgaggag agcacacaga ggaaagatat ggcctgattc gcgggaggca aaacaagagt
     2281 cttggtttca ggtgggagcg atgggcgagg ggatgggagt cttaaggggt gaggtagtat
     2341 tggggggtct ggaggatgat tataggacct atgacgacag gggggatgag gtggacaact
     2401 ccttcaggat aaaaaacact atattctttc tctcccgccg gcacggcggg acaagaaggc
     2461 cgccggatct tgggaggctc caagagccgg cggcagtctt ttctctcttt cctcctctct
     2521 ctgcgtcctc tgtgagcttg agagaggaac gcaagcgacg aacgggcgag agataatgcc
     2581 tttgcctcta acgccgtatc gcgacccggc cgtattcccc gtcgtagccg ggttcgatgg
     2641 tgacgtcgcc ggcccggacg cgggcgatcg cgtcggctat atcggggtgg ccggtgcgtg
     2701 tgatgtcggt tagtggtgcg tccatgagga tggcaagctc gggcccgagc ttctctaaaa
     2761 ggtcgaagta aaccttgtcc accttcttcg tctgggctcc cgctcgcagt gtgtcggcga
     2821 tgatctccct gagagggatg agagaggtga aaggtttcgc agctttcggg cggaagccgg
     2881 cctccctgtc ggcgagggcc tcgacgcggt tgagaacgcc caacgtgagc ttcttcccgc
     2941 agacagggca gcggtactcg gcgtctgctg cctggcgggg tgtcatcctc accttgcagg
     3001 ggcgatggcc gtcgtagtgg tacttccctt cctcgggaaa gaactcgatg gtgccgtcga
     3061 agccttccct ggtcgtgact gcgcgagcta tcccctcgaa cgacaggtcg gtgtttaaga
     3121 tgttcgcctc tcggccgatc ttctgcgggg aatgggcgtc ggaattggag acgagggtaa
     3181 ggcggtcgag gggcgagacg cgccagttca tcggagggtc cgaagagagg cccgtttcga
     3241 cggcatgaac gcggggtgtg agttcctcga agcattcctc gagagagtca aagcccgagg
     3301 ccgagccaag gacggagaag tggggtgtcc acacatgggc ggggatgaag agcgcccggg
     3361 gacaatcgtc gatcaccatg gcaaggagcc tcttggcgtc gaggccgagg ataggccttc
     3421 cgtcggaaga gatgttcccc accttcgaaa gccggttctg gagacgcagc gcgctctcca
     3481 tgtcgtcgaa gaggacaatg cagtgggcct ttcgcgtctt tccgttcctg gtgaagatgc
     3541 agcttatctc cacgctcggg agaaagaaga cctctgccac gcaggagcgg ggtataatat
     3601 cctccctcat gtcggcgcgc agcgcaaaca gaccgttgcc ggcagggtcg agcttgcgtg
     3661 caagctccct gaaccagccc gggtgtatcg cgtctcccgt gccgatgacg gtcactcctt
     3721 taagctgcgc ccacttccag agcccctcgg gtgtcatgtc ccggctcgtc gcgatggagt
     3781 aatgggaatg catatgaagg tcggcgataa atggcatcga tatcctttcg gtgtcatacg
     3841 atagcatcga acgggggtgt ttgcaaggcg gtgtcgcggt acaggaagga cgtcgggcgg
     3901 acaggcggcg gatggcattc acgtttcagg agatgctcag cggaggtcct gtttgccgga
     3961 tatgtggtcg atggctatcc tgacgacggc ggtgagcgcc agcttgtctt cggggaacgg
     4021 cccgtagccg ccttcgggct gatacttctc catgagagct cggagggcgc gcaatctcgt
     4081 ctcacggtcc tcaacgacaa gcgctcttcc cttgacgatg acgctctggt aacgataacc
     4141 ggcccggcac gggctcgcgt cgctcctgac atatccggcg gggaggtcga tctcgaagca
     4201 gacccgcgcg tctctcttca cgtcgtccat cttctctccc tcgcgggcgg agtgaaagta
     4261 tatgtcgccg tcgagataga cgaagctgag aggtttgatc atgggagacc cgtcggcgcc
     4321 gacggtgccg agccttccca cggggcagtg cctcaagagg ccggcgagga cggccggatc
     4381 ccttatctcc tttttagttg cgcgcatgtc tgttatctcc ctttccttcc tctcttatac
     4441 cataaccggg cagtgtcatg gagtgtggtg gagacggatg ttgttgttaa gtgccccggc
     4501 catcactatg gggatagttt gtcaaggggg tgcagcgggt gatgatggcc ggggccggta
     4561 ggaccaggaa ctgcgtctgt ctcagtgatc gaaacaaaaa gcgtctcaag aatggatccc
     4621 acccttgaga cgcccctgtc ttacccgcac gtcaacgtgt tgaggtgcgg gtgaacccta
     4681 cggactgtac aagtggatgg aggcccctgc tggtaacagg gacgcagcga atcttcgcga
     4741 aaccgccttg ggcgtgctat aatgaatgtt gctccacaac ggaaagacaa catcgatcag
     4801 caaaaggagg ctctcatgag caaggatgaa aagaaagaag agaaagtatc gggcatcagc
     4861 aggcgtaact tcatcacggg aacaggcgtg gccatggcgg gagccctggc gacgggagcg
     4921 ttgctgccgg gcaaggtgga ggcggtgccg cctccgaaga aatggggcag gacaacggat
     4981 gtcctcatta tcggaacagg ctactccggt cttgccgcgg ccatcgaggc ccacgatgcc
     5041 gggtgcaagg tcgccatcat cgagaaggcg gtcgtgatcg gcggcaattc cgcgatcgcg
     5101 agcgggatct acaactgttc ggagcccgac gtgcaggcga agtacgggat aaaggattcc
     5161 ccggagcagc actatcagca gacgctggcg gccggcgact tcaggggcga cccggagaag
     5221 gtgaaataca tgacgtatca cgccctcgag ggacgcaaat ggctggaaaa gcagggggtg
     5281 cagttcgacg agaaacccta tacggccgtc ggagccctct gggcgaggag cttcgaccct
     5341 gtcaagaaag gcaggggagg ttcgatcata cgggccctga aggcccaggt cgatgccaga
     5401 aagatccccg tcatgatgaa tgtgaagctc aaggccctcg tccggcagaa accccttgag
     5461 ggtgatatca tgggagcggt ggtcacggag aaggggaaag acgtctactt caaggcccag
     5521 aaggccgtca ttcttgcgac gggcggtttc gccgccgacg ttgcgatgcg ctccaagcat
     5581 gatccgcgcc tgaccgccga ggttccgacg acgaatgtgc catgggcgac gggagaggcc
     5641 atcgtcatcg ccgagaacga aggggccgat gtccacggga tggattatat ccagatgctc
     5701 atagcctgca actacttcac gaagaaatac ggttcgctga cgaatctcgg tgtcgatcac
     5761 gccctcttcg tcaacctcga cggaaagagg ttcgtcgctg aggaccagcg ccgcgacgtc
     5821 atggcggagg ctgttctcaa gcagaagaac aaggtgctcc tctgggtcgc ggatgaaaag
     5881 tgctccaaga ggttcaaccc gaagatgacg gaggagatca tcaaggacgg tctcgccttc
     5941 cgcgcgaaca cgctcgagga gctcgcgaag atcttgaacg cgaagcttga ggtcccggaa
     6001 caggccttca tcgaatccgt cgcgaagtac aacgagagcg tcacgaaagg caaggacgag
     6061 gagttcggca aaaagccgca gaacctgaag cccgtcacca ccgctccttt ctatgcgagc
     6121 cctacccagg ccggtgtgca ccacacgatg gggggcatcc agacaaaggg gaccacgtgc
     6181 caggtgatcg acagggagaa caagatcgtc ccgcggctct acgcggcagg cgaggtcacg
     6241 ggcggcgtcc acggtgcgaa cagggtcggc gggaacgcga cggtcgactg catcgtcttc
     6301 ggaaggaacg ccggcatcaa cgcggcgaag gaaaagtcct ggagctgaac tccggacgat
     6361 tcgggatgga ataggtgaca aggggcaggg agatctctct gccccttatt ttgaacggcc
     6421 ccaaaaaggg ggtgaaccgt gggacgacag ccctagcggc agggaatgac cttcaccgcg
     6481 tcgcgtccct caagggtggt cccatcctgt gtcatcccct tgagaatgcc cccggtgtcg
     6541 ccgcattgga atccggcctt ctgtacccag aagagacaga tgaggtcctg gtaaccgtcc
     6601 gcgttgacgt cccagtccca gggactgcag aaggccatgc tgtcctcgtc cccggtacgt
     6661 ccgaagctga tggtgccgag gtctatcgtg ccgagggcgt caaaggcgtt gccgccgagt
     6721 acggcaaccg gaaggagccc ccgctcgagc aggtgcagca cgttttccgc gctccacggc
     6781 ctgatgtcga tggcgagcga cgtgaggtcg gacacggcct ttgccaggaa gccatgggtc
     6841 ccgttcgcgt cctcgaaggt cccgacgatc gtccctctgt cattgatgcc ataggcgata
     6901 gtgctggtgg cccccggatg atcaagggtg ttccagatcg taccatcgta ggaaaaaccg
     6961 tggaccgatc catcctcccc ggtgtactgg ccgacaatta tgccgtggtt gttgacaccg
     7021 aaggcctctg ttgatgtcgc gtccgggtag tcgaggagga cccaggcccc gccgctggaa
     7081 taacagccgt gagacccgtt cgcgtcggtg caacctccga cgatcaca
//
LOCUS       DAOQTR010000031         7022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_11824_length_7022_cov_3.423999, whole genome shotgun sequence.
ACCESSION   DAOQTR010000031 DAOQTR010000000
VERSION     DAOQTR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11824_length_7022_cov_3.423999"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKN85_01455"
     CDS             complement(<1..683)
                     /locus_tag="PKN85_01455"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNT43094.1"
                     /translation="MKHMKVLSPKRMARYDEYAIKTWGIPSAVLMENAGRNTYRLMKE
                     RYLAGGETIAVICGRGNNGGDGFVIARYALIGGFRVRAYLIGRKSDLKGDAALNMGLF
                     ESLSGEVIECVEKTKPVRTGIGEADIIVDAIFGTGLSKPVGGMEKTVIDAINASGKPV
                     IAVDIPSGIDGATGKALGSAVHGLHTFTYAYPKPGQVLSPGADHCGSLTVIDISIPSF
                     VEKEVGFDG"
     gene            complement(694..1017)
                     /gene="acpS"
                     /locus_tag="PKN85_01460"
     CDS             complement(694..1017)
                     /gene="acpS"
                     /locus_tag="PKN85_01460"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNT43095.1"
                     /translation="MIGIDIVDIARIGGVLERHGERFLEKVFSASEIARAHGTKRLQE
                     YLAGRFAAKEAFMKARGGRLPWRDIEVLSEKGRPFIVYRGRRYDGVSISHERSYAVSV
                     VICGD"
     gene            complement(1134..1850)
                     /locus_tag="PKN85_01465"
     CDS             complement(1134..1850)
                     /locus_tag="PKN85_01465"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNT43096.1"
                     /translation="MPELMVNIDHVATLREARGIHYPDPVFAAGIAEMAGATGIIIHL
                     REDRRHIKDRDLRILRDVVRTKLNLEMAATPEMTKIAASTKPDMITLVPEKRQELTTE
                     GGLDVIKFAGKIEKVIAAVKKRGIAVSLFIDPVEPQIRASAGIGADMIEIHTGSYAEA
                     RTARMTKRELDKVIRAAVLGKELGLGVNAGHGLHYHNAREIAVIPQIDELSIGHSIIA
                     RALFVGLDRAVKDMIEVIKK"
     gene            complement(1916..2611)
                     /locus_tag="PKN85_01470"
     CDS             complement(1916..2611)
                     /locus_tag="PKN85_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNT43097.1"
                     /translation="MMRTVFERYVLKDWKLKLLSLGLAIMLWYTVFQIGEPKKDITVP
                     LTISGLAKNMVVMKMDPERVIVTVSGRVSMLKDIKERDVTAILNMNGAKEGPVVFEMS
                     KTSVAVPKGIDIVEVRPGTVKVTLDRIIEKKLKVTPVLDKKWTGRYDVIHTSPQFVIA
                     EGPRKVLEKITDIETLPANGSLNRSEEVVDVGFNIEELSRVKVRPDSAHMILKRRGGR
                     ETTGDGTGPSIGR"
     gene            complement(2631..3371)
                     /gene="cdaA"
                     /locus_tag="PKN85_01475"
     CDS             complement(2631..3371)
                     /gene="cdaA"
                     /locus_tag="PKN85_01475"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNT43098.1"
                     /translation="MIPNVRWQDILDIIVVAFIIYRIFVVIKGTRAIQLLLGLVIVMF
                     AFAIAKRLELFTLSWIFNSFIGSIVFVVIVIFQDDLRRLLLALGRSPFFRKISYVKET
                     LFLDELMNACLVMKKRTMGALIVIEREVGLEEFMEAGVRFDAEVNAELVVSIFQFASP
                     LHDGALIIREGRIVSAGCVLPLTTADEIDKTLGTRHRAAMGITEVTDAVSIVVSEERG
                     VISYAQHGQIHGNISTDELRRVLKEVLS"
     gene            complement(3368..4186)
                     /gene="folP"
                     /locus_tag="PKN85_01480"
     CDS             complement(3368..4186)
                     /gene="folP"
                     /locus_tag="PKN85_01480"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNT43099.1"
                     /translation="MAWAFQCKTTPVIMGILNVTPDSFSDGGRHRSTEDAVDHARLMI
                     EEGADIIDVGGESTRPFSEPVAEKEEIRRVIPVIERLHEIPGAFISVDTYKANVAREA
                     LKAGADMVNDISGLTYDDAMAGVIAGNDAHAVIMHIKGTPRNMQENPWYDDVVGEISA
                     FLHERVEYAVRSGVDREKIVIDPGIGFGKRVEDNLRILKMLAAFKELGRPVLIGTSMK
                     SFIGKLTDMPLEERVEGTLASLAVAIMNGADVLRVHDVRRANKVLKIVKAVMDA"
     gene            complement(4164..5942)
                     /gene="ftsH"
                     /locus_tag="PKN85_01485"
     CDS             complement(4164..5942)
                     /gene="ftsH"
                     /locus_tag="PKN85_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNT43100.1"
                     /translation="MLVIILLGLFIFQIYNKPKKNLESIAFSDFSEAVRADKVASVLM
                     QGRNITGTYKDGKEFKTFAPEDPDLVKTLREKNVKITAKPDEESGLWQSIFISWFPML
                     LLIGVWIFFMRQMQSGGGKAMAFGKSKARLFTGRENRVTFHDVAGIDEAKEELQEVID
                     FLVDPKKFTKLGGRIPKGVLLVGPPGTGKTLLARAIAGEANVPFFSISGSDFVEMFVG
                     VGASRVRDLFNQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGF
                     ESNEGVIVMSATNRPDVLDPALLRPGRFDRQIVVPTPDVKGRADILKVHTRKTLVDEN
                     VDLSIIARGTPGFSGADLANLVNEAALIAARRDKKAVEMDDFEHAKDKVLMGVERRSM
                     IIPLEERRLTAYHEAGHALVAKMIPGTDPIHKVTIIPRGRALGLTQQLPIDERHTYSK
                     DYLLNNISILLGGRVAEELVIGQLTTGSGNDIERATQIARKMVCEWGMSDKLGPLNYG
                     KNEEHIFLGREIARHRDFSEQTAQGIDEELKELVGRCYSQAKDIISKHISSLHALAKA
                     LLEKEVLDGLEIDAIIRDTDGVGVPV"
     gene            6228..>7022
                     /locus_tag="PKN85_01490"
     CDS             6228..>7022
                     /locus_tag="PKN85_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT43101.1"
                     /translation="MDTRTPTVLALIASPRILGNCETFAKEVSRNIPEAHRLRLLRLT
                     SLKIGRCRACYGCVVGRKCPVEDDMAFLLGEIANADAFIIASPVYYLGANASIKAVLD
                     RGFLFFDILEKTHGKPAVLLNFYGMPGRIGAAPQTLETLAAFLGLSVKASLSIEAALP
                     GEALMNKANAGRATRLGKALFSRGKRRRAGGCPFCGCEIIRVVPTGFVCTVCHGSFTI
                     GPQGRFVKGKDGGILGSPEHMLLHREWLRASAAHAVAMNSTLATAFA"
BASE COUNT         1249 a         2130 c         2045 g         1598 t
ORIGIN      
        1 tccccgtcga agccgacctc cttctcgacg aaggaaggga tggatatgtc gatgaccgtg
       61 aggctgccgc agtggtcggc gccgggactt aagacctgcc ccggcttcgg gtacgcgtag
      121 gtgaaggtgt ggagcccgtg gacggcgctt cccagggcct tgcccgtggc cccgtcgatg
      181 ccggagggta tgtcgacggc gatgacgggc tttcccgagg cgttgatcgc gtcgatgacg
      241 gtcttttcca ttccgcccac gggtttcgaa aggcccgttc cgaagatggc gtcgacgatg
      301 atgtccgcct caccgatacc tgtcctgacg ggtttcgtct tttccacgca ttcgatgacc
      361 tctcccgaga gggattcgaa aagccccatg ttcagggccg cgtcaccttt caagtccgac
      421 ttcctcccga tgaggtatgc acgcacccgg aaaccgccga tcagggcata gcgggcgatg
      481 acgaacccgt ctccgccgtt gttgccgcgg ccgcagatga cggcaatggt ctcgccgccg
      541 gcgaggtagc gttccttcat gagccggtac gtgttccttc cggcgttctc catgagcacc
      601 gccgagggga tgccccaggt cttgatggcg tattcgtcat agcgtgccat tctcttcggt
      661 gaaaggacct tcatgtgttt catgccccct ttgtcagtcg ccgcagatga cgacggatac
      721 cgcgtacgat ctctcgtggg agatgctgac cccgtcataa cgcctgcccc ggtatacgat
      781 gaaaggcctg cctttttcgc tcagcacctc gatatcccgc caggggagcc gcccgccccg
      841 cgccttcatg aaggcctcct tcgcggcaaa cctcccggca aggtattcct gcaagcgttt
      901 cgttccatgc gcgcgcgcga tctccgaggc cgagaacacc ttctcgagaa atctctcccc
      961 gtgcctttcg agcaccccgc caatccgcgc tatatcaacg atatcgatcc caatcatctg
     1021 aataaccaat gaccaaattc caataaccaa ttgaccaatg atcggtaacc ggacttggtc
     1081 tccaaagggg aagaactgtc gttacgtttg gtcaattgtg atttggttat tggtcatttt
     1141 tttatcacct ctatcatgtc ttttaccgct ctgtcgaggc cgacgaagag ggcgcgggct
     1201 attatgctgt ggccgatcga cagttcgtct atctggggga tcacggcgat ctccctcgcg
     1261 ttgtggtagt ggagcccgtg gccggcgttg acgccgagac cgagttcttt accgagcact
     1321 gccgcccgga tgaccttgtc gagctctctc ttcgtcattc tcgcggtgcg ggcctcggcg
     1381 taggaacccg tgtggatctc gatcatgtcg gcgccgatcc cggccgatgc cctgatctga
     1441 ggctccacag ggtctatgaa gagggaaacg gctatgcccc gcttcttgac cgcggctatc
     1501 accttctcga tcttcccggc gaacttgatg acgtcgaggc cgccctcggt cgtgagttcc
     1561 tggcgcttct cggggacgag ggtgatcatg tcgggtttcg tggaggccgc tatcttcgtc
     1621 atctccggcg ttgccgccat ttcgagattg agcttcgttc gcaccacgtc gcgaaggatc
     1681 ctgaggtcgc ggtccttgat gtgcctgcgg tcttcgcgaa ggtgtatgat gatccccgtg
     1741 gccccggcca tctccgcgat acctgccgcg aagacgggat cgggatagtg aatgccccgt
     1801 gcttcccgca gcgtggccac gtgatcgatg tttaccatga gctcaggcat cttccttcct
     1861 ccgaaagctg tatttgagcg gtcccggccc gctgctcacc cgaagacccg gggcgttatc
     1921 gtccgatcga ggggccggtc ccatccccgg tggtctccct tccgccacgg cgcttcagta
     1981 tcatgtgtgc cgaatcgggt cgcaccttga cccgggacag ctcctcgatg ttgaatccga
     2041 catcgacgac ctcttcgctg cggttcagac tgccgtttgc cggaagcgtc tctatgtccg
     2101 taatcttttc gaggaccttg cgcggaccct cagcgatgac aaactgcggg cttgtgtgaa
     2161 tgacatcgta gcggccggtc cattttttgt ccagcacggg ggtcactttg agcttcttct
     2221 cgatgatcct gtcaagggtg acctttaccg tacccggccg gacctccact atatctattc
     2281 ccttcggcac cgccacgctc gtcttggaca tctcgaagac caccgggcct tctttcgccc
     2341 cgttcatgtt gaggatggcc gttacatcac gctccttgat gtccttgagc atcgagacgc
     2401 ggccgctcac ggtgacgatg accctctccg ggtccatctt catcaccacc atgttcttcg
     2461 ccaggccgga gatggtcaga ggaacggtta tatccttctt cggttctccg atctggaaca
     2521 cggtatacca gagcatgatg gcgagtccca gcgaaaggag cttcagcttc cagtccttga
     2581 gcacgtatct ttcgaagacg gttctcatca tagccgtttc ggtcccttct tcagctcaag
     2641 acctccttga gcacacgcct gagttcatcg gtgctgatat tgccatggat ctgcccgtgc
     2701 tgcgcgtagg atatcacgcc tctctcctcc gaaacgacga tggaaacggc atcagtgacc
     2761 tcggtgatgc ccatggcggc ccggtggcgg gtgcccaggg tcttgtcgat ctcatcggcc
     2821 gtcgtcaggg gaagcacaca tcctgccgag acgatccttc cttccctgat tatcagcgct
     2881 ccgtcgtgca gcggtgacgc gaactgaaag atgctgacaa cgagttcggc gttcacctcg
     2941 gcgtcgaagc gcacccctgc ctccatgaac tcttccagcc cgacctcccg ttcaatgaca
     3001 atgagggcgc ccatggtacg ctttttcatg accaggcagg cattcatcag ttcatcgaga
     3061 aaaagggtct ccttgacgta gctgatcttg cggaagaaag ggctcctccc gagggcaaga
     3121 agaagcctcc tcaggtcgtc ctggaatatg acgatgacca cgaacacgat ggagcctatg
     3181 aagctgttga atatccagct taaggtaaaa agctccagcc tcttcgcgat ggcgaaggcg
     3241 aacatgacga tcacgaggcc gagaaggagc tgtattgccc gcgtcccctt tatgacgacg
     3301 aagatgcggt agatgatgaa ggcgacgaca atgatatcga ggatgtcctg ccatctgacg
     3361 ttgggtatca tgcgtccatt accgccttca cgatcttgag gaccttgttt gcccgcctca
     3421 cgtcatggac cctcagcaca tcggccccgt tcatgatcgc gacggcaagg cttgccaggg
     3481 tcccttccac cctctcttcg aggggcatgt ccgtgagctt tcctatgaag gacttcatcg
     3541 aggtgccgat aaggacaggt ctgcccagtt ccttgaaggc tgcgagcatc ttgaggatgc
     3601 gcaggttgtc ctccacccgt ttcccgaaac cgatgcccgg gtcgatgacg atcttctccc
     3661 tgtcgacccc gctccgcacg gcgtattcca cccgttcatg gaggaacgcg ctgatctcgc
     3721 cgacgacgtc atcgtaccag gggttctcct gcatgttgcg tggcgtgccc ttgatgtgca
     3781 tgatgaccgc gtgggcgtca ttcccggcga tgacccctgc catggcgtcg tcgtacgtga
     3841 ggccgctgat gtcgttgacc atgtcggctc ccgccttgag ggcttcccgg gccacgttcg
     3901 ccttgtacgt atcgacggag atgaacgcgc cgggtatctc gtgaagcctc tctataacag
     3961 gtattacccg acggatctct tctttttcgg ctacaggttc tgaaaagggc cgggtcgatt
     4021 ctccgccgac gtcgatgatg tccgcgcctt cctcgatcat cagccgggcg tggtcgacgg
     4081 catcctccgt gcttcggtgt ctgcccccat cggaaaaaga gtcaggggtg acattgagga
     4141 tccccatgat gacgggggtt gtcttacact ggaacgccca cgccatcggt atccctgatg
     4201 atagcatcga tctccaggcc gtcgaggact tccttttcga ggagtgcctt cgccagggcg
     4261 tgaagggagc tgatgtgctt ggagatgatg tctttggcct gtgagtaaca cctgccgacg
     4321 agctccttca gttcttcatc gatgccctga gcggtctgtt cgctgaaatc ccggtggcgg
     4381 gcgatctccc tgcccaagaa tatgtgctct tcgttcttgc cgtagttgag gggtcccagt
     4441 ttgtcgctca tgccccactc gcagaccatc ttccgggcga tctgggtggc gcgttcgatg
     4501 tcgttgcccg accccgttgt gagctgaccg atgacgagtt cctccgcgac gcgccctccg
     4561 aggaggatgg agatgttgtt gagaaggtaa tccttggagt acgtatggcg ctcgtctatg
     4621 ggcaactgct gcgtgagccc cagcgccctg cccctgggga tgatcgtgac cttgtgtatc
     4681 gggtcggtgc cggggatcat ctttgccacg agggcgtggc ctgcctcgtg ataggcggtc
     4741 agccgtcttt cctccagggg gattatcata ctgcgcctct cgacccccat gaggaccttg
     4801 tccttggcgt gctcgaagtc gtccatttcg acggcctttt tgtcacgccg cgcggctatg
     4861 agggcggcct cgttgacgag gttggccagg tcggccccgg agaaccccgg cgtcccccgc
     4921 gcgatgatcg agaggtcgac gttctcgtcg acgagggtct tgcgtgtgtg gaccttgagg
     4981 atatccgccc tgcccttgac gtcaggagtg ggcacgacga tctgcctgtc gaaacggccg
     5041 ggacgaagca gggccggatc gaggacgtcg ggtctgttcg tggcggacat gacgatgacg
     5101 ccttcattgg actcgaaacc gtccatctcc acgaggagct ggttcagtgt ctgttcccgc
     5161 tcgtcgtggc cgcctccgag ccccgccccg cggtgtctcc ccacggcatc gatctcgtcg
     5221 atgaagatga tgcagggggc attcttcttc gcctggttga agaggtcgcg gacgcgcgag
     5281 gcgccgacgc cgacgaacat ctcgacgaaa tcggacccgc ttatgctgaa aaagggcacg
     5341 ttggcctcac ccgctatggc gcgggcaagg agtgtcttgc cggtgccggg aggccccacg
     5401 agaaggaccc ccttggggat gcgtccccca agtttcgtga atttcttcgg gtccacgagg
     5461 aagtcgatga cctcctggag ctcttccttt gcctcgtcga tccccgccac atcgtgaaag
     5521 gtcaccctgt tctcgcggcc ggtgaagagc cgtgccttgc tcttgccgaa ggccatggcc
     5581 ttgccgcctc ccgattgcat ctggcgcatg aagaagatcc agacgccgat gagcaggagc
     5641 atggggaacc aggatatgaa gatggattgc cacaatccgg attcctcatc gggtttcgcg
     5701 gtgatcttca cattcttttc ccggagcgtc ttcacgagat ccgggtcctc gggcgcaaag
     5761 gtcttgaact ctttgccgtc cttgtacgtt ccggtgatgt tacgtccctg catcaggacg
     5821 gacgcgacct tgtcggcgcg caccgcctcc gagaaatcgc tgaaggctat gctttccaga
     5881 ttcttcttcg gcttattgta gatctggaag atgaagaggc cgagcaatat gatcaccagc
     5941 acggtgatga tgctcttgtt tgtgggtcct tgtccgctag cttttgacat aggtcagagt
     6001 gtaccacaag aaggccataa ttttcaatat tgtcaggcat taacagtcgg gtttcaggtc
     6061 ttggggttaa gggaacgggg aaaagacaat aaaaaccttt gcatttcaag gcacgggagg
     6121 tcatgcgcct cccggggcct gccccctgat cccttctttc tcccttgctt ttgacgccct
     6181 ggtgtgcaat aagaacgata cgggtcgcag ggagggacca ggatgctatg gataccagaa
     6241 caccgacagt tcttgcgctc atcgcttcac cgaggatcct cggaaattgc gaaaccttcg
     6301 ccaaggaggt gtcccggaac atcccggaag cgcacaggtt gaggctcctg aggctcacat
     6361 cgctgaagat aggaaggtgc cgggcctgct acggatgtgt cgtgggcagg aagtgtccgg
     6421 tggaggacga catggccttc ctcctcggtg agatcgcgaa cgccgacgcc ttcatcatcg
     6481 cctcccctgt ctattacctc ggggcgaacg ccagcatcaa ggccgttctc gacagggggt
     6541 tcctcttctt cgacatcctc gagaagaccc acggcaaacc ggcggtcctt ctcaatttct
     6601 acggaatgcc ggggcggatc ggagcggcgc cccagacgct cgagaccctt gcggcctttc
     6661 tcgggctgtc cgtcaaggcg agtctttcca tcgaggcggc gctccccggc gaggccctca
     6721 tgaacaaggc caacgccgga cgggccacgc gccttggaaa ggcgctcttc tcacggggga
     6781 agcgaaggag ggcagggggg tgccccttct gcggctgcga gatcatccgc gttgtcccga
     6841 caggcttcgt ctgcaccgtt tgccacggtt ctttcaccat cgggccgcaa ggccggttcg
     6901 tcaaagggaa ggatggtggt atcctgggtt ctcccgaaca catgctcctt caccgcgaat
     6961 ggctgcgggc cagcgccgcg cacgcggtgg cgatgaacag cacgctcgcg acggccttcg
     7021 cg
//
LOCUS       DAOQTR010000032         6993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_11895_length_6993_cov_3.332517, whole genome shotgun sequence.
ACCESSION   DAOQTR010000032 DAOQTR010000000
VERSION     DAOQTR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11895_length_6993_cov_3.332517"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2246
                     /locus_tag="PKN85_01495"
     CDS             <1..2246
                     /locus_tag="PKN85_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43102.1"
                     /translation="DWFVSIGHNPFPWYSFGGPNLSHDDRVSIQIEDHYYDRTDAAVV
                     FKRRDNWTGDEKFVYHGNDGTSMPWNDTAQLNYLNPEVREAMIRTIIHVARKFPVIRF
                     DAAMTLTKKHYQRLWFPEPGTGGAIPTRSEHSLTYDDFSKAMPAEFWREVVDRVAAEA
                     PDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLRDEENSKYRLVMKNTLEFDPQIL
                     RRFVNFMNNPDERTAVDQFGTGDKYFGVCTMMVTMPGLPMFGHGQIEGYGEKYGMEYR
                     RAYWDETPNQYLVERHEREISPLLHHRYLFADVENFLLYDFFAPEGTVNEDVFAYSNR
                     FGDARTLFIFHNRYATARGWIHRSAAFSVKEGPGDARRLVQKDLMEGLGLAADGGYFT
                     LYRDHVSNLEYLRENRELAEKGLYVELQAYQYHVFLDFRQIQDNQWQHYAQLCRYLGG
                     RGVPSIEETMREIFLQPVHQYFRELVNPGFFRYVLTTHRDVVNRKVLLPPDELTDEVT
                     RKLADLCREIKTYAGSSQDEQAIIREVTRKLTTLITSHPFTEHIAESETDIRSVLSRE
                     LSAAHLAWLFVHVLGGLIDGAGPRAEVSRSWIDEWLFGRIIGETLEELGYDDSDRHSA
                     QLLIKIFTTHQRWCEDRDPRSVLASLMADGDVHYFLGVNRYQGVLWFSKEAFEDMMAW
                     MHFTAGVLILSREGLEERRMFEELRDSFKAIDTLHQASLSSDYQLEKLMTILKENGQL
                     TKLQKSD"
     gene            complement(2306..2488)
                     /locus_tag="PKN85_01500"
     CDS             complement(2306..2488)
                     /locus_tag="PKN85_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43103.1"
                     /translation="MKEPIGFCDLSCKYASMPKETGLDGAGSCRTFVAIRCSLKKTLV
                     HKNMPCKDKVFRKASK"
     gene            complement(2540..2875)
                     /locus_tag="PKN85_01505"
     CDS             complement(2540..2875)
                     /locus_tag="PKN85_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43104.1"
                     /translation="MKERLRSEAKRLPWTLAKLVGGLISAIGFGGAVVVLTRRPDASW
                     GGVLAWVLAGVIGLVLFLASSRSLARRTEAEESPPTGKERMKASALSWMILLILGALF
                     LVIVLIVAA"
     gene            complement(2887..3135)
                     /locus_tag="PKN85_01510"
     CDS             complement(2887..3135)
                     /locus_tag="PKN85_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43105.1"
                     /translation="MIIEKASIVFGWCTVINLIVLMIWFLAFSRAHEMLYRIHVQWFR
                     ISRETFDAIHYAGMAFYKVCIILFNLVPMIALRIALQA"
     gene            complement(3350..4159)
                     /locus_tag="PKN85_01515"
     CDS             complement(3350..4159)
                     /locus_tag="PKN85_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNT43106.1"
                     /translation="MAPLSRSRSSSIQMVKAVLVLAMIIALPAVGFAARPLLTDDAGT
                     LGKGGFQVELGFETSDHRTDDDGVVTREDASSTSATLSYGILDNLDVLLGMPYEWSKV
                     REDGDTIRNSDGIADLTLEAKWRFFEKGGFAMALKPGVSFATGDHKRGFGTGRTTYGA
                     LFIATQDIGPVSLHLNAGYRRNENDTDERKDIWGGDFAAVVTVAKPLKLVANVGTKTS
                     TDRTAATDPAFFLGGLIYSITDKIDLDLGYKRGLNKAEPDNAYIGGLTIRF"
     gene            complement(4223..4591)
                     /locus_tag="PKN85_01520"
     CDS             complement(4223..4591)
                     /locus_tag="PKN85_01520"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HNT43107.1"
                     /translation="MCVALIGGMDRLERDYEAEALKHGVELRVFTKTKTGLVTRLKAV
                     DVMVMFTGKVSHQLKREAVSAAKSRSIPVIMVHSCGVCSLRECLQRLAASGQRDAGVS
                     GCAGSCKDAHCRRAADRAVA"
     gene            complement(4623..4787)
                     /locus_tag="PKN85_01525"
     CDS             complement(4623..4787)
                     /locus_tag="PKN85_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43108.1"
                     /translation="MNGSIGELTAVLVIVGAVVVLAARSVYRLVTGGEAGKCCGCSPC
                     SCRDDSKETR"
     gene            complement(4801..6951)
                     /gene="feoB"
                     /locus_tag="PKN85_01530"
     CDS             complement(4801..6951)
                     /gene="feoB"
                     /locus_tag="PKN85_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNT43109.1"
                     /translation="MSSNLIKVALIGNPNVGKTSVFNELTGLNQQVGNYPGITVEKKQ
                     GVCKLSESVKAKIIDLPGTYSLTAYSAEELVARNFIVDEKPDVVVDIIDSSNLERNLY
                     LTVQLMETGAPLVLAFNMSDEARTRGYEFDLERFSRFFNAPVVRTVGHKGEGMDELMK
                     QIVTVALQEKEPRDTPSVNYGEELEEEIGKMIRLLDEEPLLAEKYGARWLSVKLLEND
                     RDVLKKVSSDAVRAQLAKSSSHVEGILGEGPETIIASSRYGFISGACQEAVHSTVEVL
                     HTISDRIDTVITNRVLGIPIFLGLMYLVFYLTFTLGDPPMGWIEGLFGWLGGKIGALW
                     PEGSESLLRSLIVDGIIGGVGGVIVFLPNILLLFFAIAILEDSGYMARAAFIMDKLMH
                     RIGLHGKSFIPMLIGFGCSVPAIMATRMLENRRDRLVTMLVVPLMSCGARLPIYALII
                     PAFFPQAWNAPMLWIIYVIGILLAVLSAKALRSTIFKGESVPFVMELPPYRLPTLRGV
                     LTHMWQRGWLYLKKAGTVILGISILLWALTAFPGLPQEQAERFEKEKQTVEATVTDEE
                     KKKEKIISIENMMREASLKGSIAGRIGHALEPVLKPMGFDWKIGTALIGAFAAKEVFV
                     AQLGIVYSVGKEANEESSELRDQLRANYTPLVAFCIMLFCLVSAPCMATIAVTRRESN
                     SWKWALFQLAGLTVMAWILTVIVFQAGRLLGIGI"
BASE COUNT         1555 a         2107 c         1829 g         1502 t
ORIGIN      
        1 ccgactggtt cgtttccatt ggacacaacc cttttccttg gtattccttc ggaggaccca
       61 acctctccca cgatgaccgc gtttccatcc agatagaaga ccattactac gaccgcaccg
      121 atgcggcggt ggtcttcaag cggcgtgaca actggacggg cgacgaaaag ttcgtctatc
      181 acgggaatga tggcacgagc atgccctgga acgacaccgc gcagttgaac tatctcaatc
      241 ccgaggtgcg cgaggcgatg atccgcacca tcatccacgt ggcccggaag tttccggtca
      301 tccgcttcga tgccgccatg acgctcacga agaagcatta ccagaggctc tggtttcccg
      361 aaccgggtac gggcggggca ataccgactc gaagtgagca cagcctgacc tatgacgact
      421 tctcgaaggc gatgcccgca gagttctggc gcgaggtcgt ggaccgtgtc gccgcggagg
      481 cgccggacac gctcctgctg gccgaggcgt tctggctgat ggaagggtac ttcgtccgga
      541 cgctcgggat gcatcgtgtt tacaacagcg cctttatgaa catgctgcgc gacgaggaga
      601 actccaagta ccgtctggtc atgaagaaca ccctggagtt cgacccgcag atactccgcc
      661 gtttcgtcaa cttcatgaac aaccccgacg agaggacagc cgtggaccag ttcggcacgg
      721 gagacaagta cttcggggtc tgcaccatga tggtcacgat gcccggtctg cccatgttcg
      781 gccacggaca gatcgaaggc tacggagaga agtacggcat ggaataccgc agggcgtact
      841 gggatgaaac gccgaaccag tacctcgtcg agcgccacga acgggagata tcccccctcc
      901 tccaccatcg ctacctcttc gccgatgtgg agaatttcct tctctacgac ttcttcgctc
      961 ctgagggcac cgtcaacgag gatgtcttcg cgtattcgaa ccgtttcgga gacgcaagaa
     1021 cactctttat cttccacaac aggtacgcga ctgcacgagg ctggatacac cggtccgcgg
     1081 ctttctccgt caaggagggc cccggggatg cccgcaggct tgtccagaag gacctcatgg
     1141 agggcctcgg ccttgccgcc gacggcggct attttactct ctacagggac cacgtttcca
     1201 acctggaata cctgagggag aacagggaac tcgctgaaaa aggcctttac gtggaacttc
     1261 aggcatatca gtatcacgtt tttctcgact tccggcagat ccaggacaat cagtggcagc
     1321 actacgcgca attatgcagg tacctgggcg gccggggtgt tcccagcatc gaggagacaa
     1381 tgcgggagat cttcctgcag cctgtccacc agtatttcag ggaacttgtc aatcccgggt
     1441 tctttcggta cgtcctcacc acccacaggg acgtggtgaa ccggaaggtc ctgttgccgc
     1501 cggacgaact gaccgatgag gtcacacgca agctggccga cctctgtcgc gagatcaaaa
     1561 catacgcggg ctcatcccag gatgagcagg ccattatccg ggaggtgacg agaaagctca
     1621 caacactgat cacgtcccac cccttcacgg agcacattgc ggagagcgag acggacatcc
     1681 gttcggtcct gtcccgggag ctttccgccg cccacctggc atggctgttc gtccatgtcc
     1741 tgggaggcct catcgacggc gcgggacccc gcgcggaggt cagcagaagc tggatcgacg
     1801 aatggctctt cggccggatc atcggggaga cgctcgagga actgggctac gacgacagcg
     1861 acaggcattc cgcccaactg ctcatcaaga tcttcacgac ccaccagaga tggtgcgagg
     1921 acagagatcc ccggtcagtg ctcgcgtccc tcatggccga tggtgatgtc cattattttc
     1981 tgggcgtgaa ccgttatcag ggcgtcctgt ggttcagcaa ggaggccttc gaggacatga
     2041 tggcttggat gcatttcact gcaggcgtcc tcatcctctc ccgcgaaggg cttgaagaaa
     2101 gaaggatgtt cgaggaactc cgcgacagct tcaaggccat cgacacgctt catcaggcat
     2161 ccctgtcatc ggactaccaa ctggagaagc tcatgaccat cttgaaagag aatggccagt
     2221 tgaccaaact ccaaaaatca gattgaaaag gcaataacgg caaaaacgca gtcggtcctt
     2281 tttgtccttc tgtttgacca attggttatt tgctcgcctt cctgaacact ttgtctttgc
     2341 agggcatgtt cttgtgaaca agggtcttct tgagcgagca ccggatcgcc acgaatgtcc
     2401 tgcagcttcc cgcaccgtcc aggcccgtct ctttgggcat cgaggcgtac ttgcatgaaa
     2461 ggtcgcaaaa accgatcggt tctttcatgg aaacttattt accatatcac gacggagggc
     2521 acaaaggttt ttccggccgt cacgctgcca cgataagaac gatgacaagg aacaaagccc
     2581 ccagaatgag gaggatcatc catgacaggg cgctggcctt catcctttct ttcccggtgg
     2641 gcggcgactc ctccgcttcc gtgcgcctgg ccagggaccg ggaggaggcg aggaagagga
     2701 caaggccgat cacacccgca agaacccagg ccaatacgcc tccccaggat gcgtccggcc
     2761 gtctcgtgag caccaccacc gcgcccccaa aaccgatggc ggatatcagt cctcccacaa
     2821 gtttggcgag tgtccagggg agccgtttcg cttcggacct caaccgttcc ttcatacctg
     2881 tcctcctcat gcctgcagcg ctatacgcag ggcgatcatg ggcacaaggt tgaacaatat
     2941 gatgcacacc ttgtagaatg ccatgccggc gtagtgtata gcgtcgaagg tctcccgtga
     3001 gatcctgaac cactgaacgt ggatccggta cagcatctcg tgggccctgg aaaaggccag
     3061 aaaccatatc atcaatacaa tgagattaat gaccgtacac cagccaaaga cgatactcgc
     3121 tttctcgatg atcacagcaa cctcctcctc cctctcactt ttttcatcaa taacagactc
     3181 aacgccatga ccgccattcc actgataagg ccgaagaggc tgtcatcgct gcttctacaa
     3241 tacattgtgg ccttgatttt tgcaagttgg ggggcggata acggctctcg ggtgacaggt
     3301 tgcaggaagg gatctgcgtc ccataaccca ttaaccattg cctgtcctct caaaacctta
     3361 tcgtcaatcc gccgatatac gcgttgtccg gttcggcctt gttgagaccg cgcttgtagc
     3421 cgaggtcgag gtcgatcttg tccgttatgg aatatatgag accaccgaga aagaaggcgg
     3481 gatcagtggc tgccgtcctg tcggtgctcg ttttcgttcc cacattcgcc acgagtttca
     3541 aaggcttcgc cacggtgaca acagcggcga aatctccgcc ccatatgtct ttcctctcgt
     3601 ccgtgtcgtt ctcattgcgt ctgtacccgg cattgaggtg caaggagaca gggcctatgt
     3661 cctgtgtggc tatgaacagc gctccgtagg tcgtccggcc ggtcccgaaa cccctcttgt
     3721 ggtcccccgt cgcgaaggat accccgggct tgagcgccat ggcgaaacca cccttctcaa
     3781 agaaccgcca cttcgcctca agcgtcaggt cggctatacc atcgctgttg cggattgtat
     3841 caccgtcttc tctgaccttc gaccactcat agggcattcc caggaggacg tccagattgt
     3901 cgagtatgcc ataggaaagg gtggcgctgg tggaagacgc atcctctctg gtcaccacgc
     3961 cgtcatcgtc ggtcctgtgg tccgacgtct cgaagcccag ctccacctgg aatccaccct
     4021 ttcccaacgt ccccgcgtcg tcggtgagga gcggccgggc ggcgaagccc accgccggca
     4081 gggcgatgat cattgcaaga acaaggactg ccttcaccat ctgtatgctg ctcgatctgc
     4141 tccgtgataa tggggccata tatctctcct tatataatag tcgaatatcc tttccatgag
     4201 ttaataggcc aggaaacttc ctttaagcca ccgccctgtc ggcggcgcgg cgacaatgcg
     4261 cgtccttgca ggaacccgcg catccggaca ctcccgcatc cctttgaccg gatgccgcga
     4321 gacgctgcag gcattcccgc aagctgcata cgccgcagga atgtaccatg atcacgggga
     4381 tgctccttga cttcgcggca ctcacggcct ctcttttcaa ttgatgggaa acctttcccg
     4441 tgaacatgac catcacatcg acggccttga gacgggtcac cagacccgtt tttgttttgg
     4501 taaagaccct caactccact ccatgtttca gcgcttcagc ctcatagtca cgctccagcc
     4561 tgtccatccc acctatcaaa gcaacgcaca tcgttacctc cttttcgtat taagaagagc
     4621 actcatctcg tttccttact gtcgtcccgg catgagcacg gggagcaccc gcagcacttt
     4681 cccgcttccc cgcctgtgac cagacggtaa acggaccggg cggcgagaac gaccacagct
     4741 cccacgatca ccaataccgc cgtcaattcc ccgatcgacc cattcatgca acacctcccg
     4801 tcaaataccg ataccgagca gccttcccgc ctggaacacg atcaccgtca gtatccaggc
     4861 catcaccgtg agccccgcga gctgaaagag tgcccacttc caggaattgc tctccctgcg
     4921 cgttaccgcg atcgttgcca tgcagggagc gctcaccagg caaaagagca tgatgcagaa
     4981 tgccacgagc ggagtgtagt tggcacgcaa ttgatcgcga agctcagaag actcttcgtt
     5041 ggcctcctta ccgacgctgt agacaatgcc cagctgtgcc acaaagacct ccttggcagc
     5101 aaaggcgccg atgagagccg tacctatctt ccagtcaaat cccatgggct taagcaccgg
     5161 ttccagggcg tggccgatcc ttcccgcgat gctgcccttg agcgatgcct ccctcatcat
     5221 attttcgatt gatatgatct tttctttctt cttctcctcg tccgttacgg tagcctccac
     5281 cgtctgtttc tccttttcga accgctccgc ctgctcttgc ggcagtccag gaaaggccgt
     5341 cagcgcccac aacaggatgg agatgccgag gataaccgtt ccggccttct tcaggtaaag
     5401 ccacccgcgc tgccacatgt gggtgagaac acccctcaac gtgggtagcc ggtacggggg
     5461 caactccatc acgaagggga cggattcccc tttgaagatg gtgctgcgca gggctttcgc
     5521 gctcaggacg gcgaggagta tccctatgac gtatatgatc cagagcatcg gggcgttcca
     5581 tgcctgcgga aagaaggcgg ggatgatgag cgcgtaaatg ggaagcctcg ctccgcagct
     5641 catgaggggg acaacaagca tcgtgaccag ccggtccctc ctgttctcca gcatccttgt
     5701 cgccatgatg gccggaacgg aacagccgaa accgataagc atggggataa aactcttccc
     5761 gtgaagacct atccggtgca tgagtttgtc catgatgaaa gccgcccgcg ccatgtaccc
     5821 cgaatcctcg aggatcgcga tagcgaagaa cagaagaagg atgttgggca aaaagacgat
     5881 cacaccgcca acaccgccga tgatgccgtc aacgatcagg gatctgagca ggctctccga
     5941 accttccggc cacagggcac cgatcttacc ccccagccat ccgaaaagac cttctatcca
     6001 ccccatggga ggatccccca gggtgaatgt caggtagaag accaggtaca tgagccccag
     6061 gaagatgggg atgccaagga cacggttcgt gatgaccgta tcgatcctat ccgaaatggt
     6121 atggaggacc tcgacggtgg agtgcacggc ctcctgacaa gctcccgaga tgaacccgta
     6181 tctgctgctg gcgatgatcg tttccggacc ctcgcccagg atgccttcga cgtgggaaga
     6241 actcttcgca agttgggcgc gcacagcatc agatgaaacc tttttgagga catccctgtc
     6301 gttttcgagg agcttcaccg acaaccatcg ggctccgtac ttctccgcaa gaagaggctc
     6361 ttcgtcaagc aatcggatca tcttgccgat ctcttcctcg agttcctccc cgtagttgac
     6421 agaaggcgtg tcacggggct ctttttcctg cagcgcgacg gtgacgatct gcttcatcaa
     6481 ctcatccatg ccctcgccct tgtgaccgac ggtcctcaca accggagcgt tgaagaaccg
     6541 ggaaaatctc tcgaggtcga actcatatcc ccgggtgcgg gcctcgtcgc tcatgttgaa
     6601 ggccagcacg agaggcgccc ccgtttccat gagctgaacg gtaagataca gattccgttc
     6661 cagattggag gaatcgatga tgtccaccac cacatccggc ttctcgtcga cgatgaaatt
     6721 cctcgccacc aactcctcgg cagaataggc ggtaagactg tatgttccgg gtaaatctat
     6781 aatttttgct ttaaccgatt cggataattt gcaaacacct tgtttttttt caacagtaat
     6841 tccagggtaa tttcccactt gttgatttaa acctgtaagt tcattaaaaa cagaagtttt
     6901 gcccacattt gggtttccaa ttaaagcaac ttttataaga tttgaactca ttgttaaagg
     6961 tcttttgtaa tactaatttc gcgcgcagtt tca
//
LOCUS       DAOQTR010000033         6887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_12130_length_6887_cov_3.662178, whole genome shotgun sequence.
ACCESSION   DAOQTR010000033 DAOQTR010000000
VERSION     DAOQTR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12130_length_6887_cov_3.662178"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKN85_01535"
     CDS             complement(<1..669)
                     /locus_tag="PKN85_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNT43110.1"
                     /translation="MAGGTLKAWVQASRMPFFVATFIPLFIGWSMAVRATGAVRPGRF
                     LIVLLGSLIVHLITNLANDYFDHLVGTDAGESIGGSRVIQEGKISPQVMLRVIIVLYV
                     IAFFIAYIIVFHFGLHLLAIPILFSAFSSYFYVAPPIRYGYHGLGELFVGINMGPVMV
                     AGTYWVVAGAPALEPLLVSIPVGIMVASILYYQSLPDMKTDEASGKMTLAVRLGRKGA
                     FRGLI"
     gene            complement(669..1427)
                     /gene="ubiE"
                     /locus_tag="PKN85_01540"
     CDS             complement(669..1427)
                     /gene="ubiE"
                     /locus_tag="PKN85_01540"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNT43111.1"
                     /translation="MEKPVDRKYPSVRAVSEAERIGMVKEMFSTITGKYDFLNHFLSL
                     RRDIAWRRFTVKKMRFFKTMRFLDVACGTADLSIDAAMKYGGISVTGADFVFEMLEHG
                     KVKVEKKGLDRRITLMQGDALELPFRDNSFDVVGIAFGIRNIPDRERALKEMLRVTVP
                     GGAVMVLEMTFIQNPLFKLVYHVYLNHILPRLAKRFSPNPAAYHYLADSIMNFPQPDA
                     FAAMMEKTGMVNVTKHPLTFGVTYLHVGVKPEGL"
     gene            complement(1412..2158)
                     /locus_tag="PKN85_01545"
     CDS             complement(1412..2158)
                     /locus_tag="PKN85_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT43112.1"
                     /translation="MKRRAIGVYAATILIIYFAWYALSLMLGSNILPDPFRVFIRGFE
                     EIATTAFWVHVEASLLRLLAALFFAFILAVPAGLVLGSNERLDGLFAPLIYLGYPIPK
                     VVLMPVIFVLFGLGDLGKVVLITMIIFFQLLITTRDSARKIDREITYSLRSLGGGRLD
                     YFFHVVWPVSLPGIFTSLRIGVGTAVAVLFLVESIATSEGLGFYIIDSWGRAEYATMF
                     VGIIALSIIGIVMYEIFELLERKFCKWKSL"
     gene            complement(2155..2913)
                     /locus_tag="PKN85_01550"
     CDS             complement(2155..2913)
                     /locus_tag="PKN85_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT43113.1"
                     /translation="MGTSGKFLAIEGLGKTFQNENDSKTVLEGVDLELAFRDCLAIVG
                     PSGCGKTTLLLAIAGLIPPTAGAITFRDGPLAAPERRIALVLQHYGLFPWKTVGANIA
                     LGARLQHIDVPEKRLRSVKRELDIEGLDHLYPAQLSGGQRQRVALARALLLNPLLLLL
                     DEPFAAIDTVTRERLQNNLLDVFAQRRFSFIIVTHNIEEAVFLGRRIVVLDGNNRGIK
                     TIMDNPGMGDPQYRNDPVFFERTTELRGILEGLE"
     gene            complement(2897..3844)
                     /locus_tag="PKN85_01555"
     CDS             complement(2897..3844)
                     /locus_tag="PKN85_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNT43114.1"
                     /translation="MKRVKSVVVFVIVFVLCLGVTAHATEKPRAFKFGTIPVLQALPL
                     FVAAEKGFFKEQGLNVDIVLFNSAMEKDIAFTSGQIAGYFGDIMTPTVLVANGTKIKM
                     VAVNFNTTADRRMFAVLASSRAKEQDLSSVARAGIATGSNTIAEYLIIRLLADKKVPR
                     ESIKLVDIKSIPIRLQMLLSGQVSAALLPEPLATLAETKGAKALADDRGHGISATVLA
                     FHTDFLDKNAASVRSFLAAVDKASAYISRHPDEVRAIMNKSCKVPEALQSSFPIPRFP
                     KVHAPSESQVMDVYRWLKEKKIVKKDLTYKDLVADGYIR"
     gene            complement(4126..5526)
                     /locus_tag="PKN85_01560"
     CDS             complement(4126..5526)
                     /locus_tag="PKN85_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43115.1"
                     /translation="MKYLMIIALLLAPLSSFGAKAQCPRYSVTMTGTAAHMGVSYTER
                     VEAYKVGGGAGYNGRWKVELFEQIITYPWQLGMAMTTNDVHDLGNGIRMQSTCTVGGN
                     VVHCRSVSDTMFLEVINNRVRMERTVPWHGQVTGSTMTWKFHLESPTEPVLSGVIAEG
                     PKEPIELTIVFPADRTRVVYSSQQPPTLELKLRAKTKPEQYAESIEWTLPEMEGVTRR
                     VIQGAPRGSVLDVIYEKMPEDNNQFGKKKVTATLKVGICTATESREVQFFYPRDLKNN
                     PGGQYPNWFYYWKQTPAAKPRGQLVNIEYGGSIYGECIHPSVPAQYTPGAGHATIHVC
                     NLAKLGSQFQNRFPVLSLQPPYHLGWETSRNIDTFAKAVIHEFVHWQCYHNFRYGKSL
                     PAIQAADTDADGLPDTVEPQYGFDPSMRQTHMSTHPELKKIKYDEEWLAYMSMSEIPM
                     GKYNEHDWARPGNQWP"
     gene            complement(5590..6465)
                     /locus_tag="PKN85_01565"
     CDS             complement(5590..6465)
                     /locus_tag="PKN85_01565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNT43116.1"
                     /translation="MTKKCLALLCAPFLLAALASCDTSSFGNISQHIGTAMTVVDQTS
                     KAARPISDEEEYYVGRAVAAKILGSYQLTTNAQLTRYVNLIGRTVALNSDKPYTYGGY
                     HFAILETNEINAFAAPGGTIFITSGMIRTTASEDELAAVLAHEAAHISKRDGIAAIKS
                     ARWTEALTVIGTTAARNYTSGEVSQLVSLFEGSIDDVFKTLVVNGYGRSQEYAADESA
                     VQTLAKCGYDPRALLSFLKRLEQRSGSSGGGITATHPATADRIENISGKIPSTAQIHP
                     RAIEARAVRYGAAVR"
     gene            complement(6462..>6887)
                     /locus_tag="PKN85_01570"
     CDS             complement(6462..>6887)
                     /locus_tag="PKN85_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43117.1"
                     /translation="AAATAALADTVTVITKENAIREYAKFFAPVKANVRYNDRLEALS
                     MEGDWYKVKCGPSIGYIHRSAVEKRVVAVPASYSRKGSSVSESEAALAGKGFNPQVEA
                     TYKKQHPEMKFGLVDSVERYPVPERDIAQFIVAGGLRQP"
BASE COUNT         1323 a         1940 c         2150 g         1474 t
ORIGIN      
        1 gatcagcccc cggaaggcgc cttttctgcc gagtctgacg gcgagggtca tcttcccgga
       61 ggcctcgtcg gtcttcatgt ccgggaggct ctggtaatag aggatggagg ccaccatgat
      121 cccgacgggt atggaaacga gaagaggttc gagcgcgggg gcgcccgcca cgacccagta
      181 tgtccccgcg accatgacag gacccatgtt gatccccacg aagagttcgc ccagcccgtg
      241 ataaccgtac cgtatagggg gggcgacgta gaagtaactg ctgaaggcgg agaagaggat
      301 ggggatggcg aggaggtgga gcccgaagtg aaagacgatg atgtaggcaa taaagaaggc
      361 gatgacgtaa aggacgatta tcaccctcag catcacctgc gggctgatct taccctcctg
      421 aatgacacgg gagcctccga tcgactcccc ggcatcggtc cccacgaggt ggtcgaagta
      481 gtcgttggcc aggtttgtta tgaggtggac gatgagcgag ccgagaagga caatgaggaa
      541 gcgcccgggc ctgacggcgc ccgttgccct gacggccatc gaccagccga tgaaaagggg
      601 tatgaaggtg gccacgaaga aaggcatcct gctcgcctgg acccacgcct tcagggtgcc
      661 gcccgccatc acaggccttc cggtttgacg cccacgtgga ggtaggtgac gccgaaggtg
      721 aggggatgct tcgtcacgtt gaccatgccg gtcttttcca tcatggccgc aaaagcgtcg
      781 ggttgaggga agttcatgat cgaatcggcg aggtagtgat acgcagcggg gttgggagag
      841 aaccgtttcg ccagccgcgg gaggatatga ttgagataga cgtggtagac cagtttgaac
      901 agcgggttct gtatgaaggt catttcgagg accatcacgg cgcccccggg gacggtgacg
      961 cgaagcatct ccttcagggc cctttcgcgg tcagggatgt tcctgatacc gaaggcgatg
     1021 ccgacaacgt cgaaggagtt gtctcgaaaa ggaagttcga gggcgtcgcc ctgcatgagg
     1081 gtgatgcgac ggtcgagccc cttcttttcg accttcacct ttccgtgttc cagcatctcg
     1141 aagacgaaat cggcgccggt gacgcttatc ccgccgtatt tcatcgcagc gtcgatggaa
     1201 aggtcggctg tgccgcaggc gacatcgaga aaccgcatgg tcttgaagaa gcgcatcttc
     1261 ttcacggtga aacggcgcca cgctatatcg cggcgcaggc tcaggaagtg attgagaaag
     1321 tcgtacttgc ccgtgatggt ggagaacatc tccttcacca tgccgatgcg ctccgcttcc
     1381 gacaccgcgc gcaccgaagg gtatttcctg tctacaggct tttccatttg cagaatttcc
     1441 tttcaagaag ctcgaatatc tcgtacatga cgatgccgat gatcgagagg gctatgatcc
     1501 ccacgaacat ggtggcatac tcggcgcgtc cccaggagtc aatgatgtag aatccgaggc
     1561 cctcgctcgt tgcgatggat tcgacgagaa agaggaccgc caccgcggtc cccacgccga
     1621 tgcgaagcga tgtgaatatc ccgggcaggc tgaccggcca gacgacatgg aagaagtagt
     1681 cgagcctgcc tcctcccagc gaccggagcg agtaggtgat ctcccggtcg atcttgcgcg
     1741 cgctgtcacg ggtggtgata aggagctgga agaatatgat catggtgatg aggacgactt
     1801 tgcccaggtc cccgaggccg aaaaggacga agatgacagg catgaggacc accttgggga
     1861 tggggtagcc gaggtagatg agcggcgcga agagtccgtc aagcctttcg ttgctcccga
     1921 ggacaagacc cgccggcacg gcgaggatga aagcgaagaa gagtgccgcg aggagcctca
     1981 ggaggctcgc ctcaacatgg acccagaaag cggtcgtcgc tatttcctcg aagccgcgga
     2041 tgaacacccg gaaagggtcg gggaggatgt tgctgccgag catgagggaa agggcgtacc
     2101 atgcgaaata gatgataaga atggtggcgg cgtaaacgcc gatggcccgc ctcttcattc
     2161 gagaccctcc aggatgcccc tgagctcggt ggtgcgctcg aagaagaccg ggtcgttgcg
     2221 gtattgcggg tctcccatcc cgggattgtc catgatcgtc tttatcccgc ggttgttacc
     2281 gtcgaggacg acgatccttc ttcccaggaa taccgcctct tcgatgttgt gcgtgacgat
     2341 aatgaaactg aacctccgct gcgcgaacac gtcgaggagg ttgttctgga gccgctcccg
     2401 cgtgacggtg tcgatggcgg caaagggttc gtcgagaaga aggagcaggg ggttgagaag
     2461 cagcgccctt gccagcgcga cacgctggcg ctggcccccg ctgagctgcg ccgggtagag
     2521 atggtcgaga ccctcgatat cgagttccct cttgacggag cgcagtctct tttcgggcac
     2581 gtcgatgtgc tggagacggg ccccgagagc gatgttggcg cccacggtct tccacggaaa
     2641 gagtccgtaa tgctggagga cgagggcgat cctgcgttcc ggggcggcga gcggtccgtc
     2701 cctgaaggtg atggctcccg ctgtcggtgg gatgagcccc gcgatggcaa gcagaagggt
     2761 ggtcttgccg caccccgaag ggccgacgat ggcaaggcag tcgcgaaagg ccagttcgag
     2821 gtcgaccccc tcaaggaccg tcttcgaatc attctcgttc tgaaaggtct tgcccagtcc
     2881 ctcgatggcg aggaacttac cggatgtacc catctgccac caggtctttg tacgtgaggt
     2941 ccttctttac gatcttcttt tccttaagcc accggtagac atccatgacc tgggactcgg
     3001 agggtgcgtg tactttcggg aagcgcggta tggggaaaga gctctggagt gcctccggta
     3061 ccttgcagga cttgttcatg atcgcccgca cttcgtcggg gtgccggctt atgtatgccg
     3121 aggccttgtc gacggccgcg aggaacgacc ggacggaagc ggcgttcttg tcgaggaagt
     3181 cggtgtgaaa ggcgaggacc gtcgccgaga tgccatggcc cctgtcgtca gcgagcgctt
     3241 tcgccccctt tgtttccgcc agtgtcgcca gaggctccgg cagcagtgcc gcgcttacct
     3301 gtcccgaaag gagcatctgg agacggatgg gtatgctctt gatatccacg agtttgatgg
     3361 attcccgggg aaccttcttg tccgccagga ggcggatgat gagatattcc gcaatggtgt
     3421 tggagcccgt agctatgccc gcgcgcgcga cggacgagag gtcctgctcc tttgcccttg
     3481 atgaggcgag gacggcgaac atgcgccggt ccgcggttgt gttgaagttc acggccacca
     3541 tcttgatctt cgtcccgttg gcgacgagga cggtcggggt catgatgtcg ccgaagtatc
     3601 ccgctatctg tcccgacgtg aaggcaatgt ccttctccat cgccgagttg aagaggacga
     3661 tatcgacatt cagcccctgc tccttgaaga accccttctc cgccgcgaca aagagcggca
     3721 gcgcctgaag caccggaatg gtcccgaact tgaacgcccg gggtttttct gtcgcatgag
     3781 ctgtgacgcc cagacaaagg acaaaaacga tgacgaagac aacaacagac tttacccgtt
     3841 tcataactac tccccttacc cttgatcgat gtgttcggtc tattagtcta gtgcgcaaac
     3901 ggggattttt caaacaaaaa ccccttaaaa ccgttcaagg gttcaagagt tcaaaggttc
     3961 aagagcagaa aaagctccag ggttcaggtg aaagaccatg cgctggtctg ccctggtcgg
     4021 cactacccgg gtctcctgtc ctgtcagact cttgaggatt ggagccttta cgggagcctt
     4081 caccacctga gacttgaaac cgtttctacc cttgaacctt ctttttcacg gccactgatt
     4141 tcccggcctt gcccaatcgt gctcgttgta cttgcccatg gggatctcgg acatggacat
     4201 gtaggcgagc cattcctcat catattttat cttcttcagc tccggatggg tggacatgtg
     4261 ggtttggcgc attgaggggt caaaaccgta ctggggctcg acagtgtcgg gcaggccgtc
     4321 agcgtcggtg tccgccgcct ggatggccgg caggctcttg ccgtacctga aattatgata
     4381 gcactgccag tgcacgaact cgtggatgac ggccttggcg aaggtgtcta tgttcctgct
     4441 cgtttcccag cccaggtggt agggcggctg aagacttaag accggaaaac ggttctggaa
     4501 ctgggacccg agtttggcga gattgcacac gtggatggtc gcatgccccg cgccgggcgt
     4561 atattgagcg ggaaccgagg gatgtatgca ctcaccgtag atgcttcccc cgtattctat
     4621 attgaccagt tgcccgcggg gtttcgccgc cggggtctgt ttccagtagt agaaccagtt
     4681 ggggtattgc ccgccgggat tgttcttcag gtcccggggg tagaagaact ggacctcccg
     4741 gctctccgtt gcggtgcaga tacccacctt cagggtcgcc gtcacctttt tcttgccgaa
     4801 ctggttgttg tcttccggca tcttttcgta aatgacatcg agaacgcttc ctctgggagc
     4861 tccctggatc accctgcgcg tcacgccctc catttcggga agggtccatt cgatggactc
     4921 ggcatactgc tcgggttttg tcttcgcccg cagcttgagc tccagtgtcg gcggctgctg
     4981 ggaactgtag acaacacggg tcctgtcggc ggggaaaacg atggtgagct ctatgggttc
     5041 cttcggcccc tcggcaatga ccccgctcag gaccggttcg gtggggctct cgaggtggaa
     5101 cttccaggtc atggtactgc cagtgacctg gccgtgccat ggtacggtcc tctccatgcg
     5161 taccctgttg tttatgacct ccaggaacat ggtgtcggaa acactgcggc agtgcacgac
     5221 gtttccccca acggtacatg ttgactgcat cctgatcccg tttcccagat catggacgtc
     5281 attggtggtc atcgccatgc ccagctgcca gggataggtt atgatctgct caaaaagctc
     5341 gaccttccac cgtccgttgt aacccgcccc gcctccgacc ttgtaggctt cgacgcgttc
     5401 cgtgtagctg actcccatgt gcgctgctgt ccccgtcatg gttacactgt atcgcgggca
     5461 ctgcgccttc gccccgaagg atgagagcgg tgccagcaag agggcaatga tcatgaggta
     5521 cttcatggaa cacctctctt cgccaatggt tatgctcttt tggttcttga ggtcaaagac
     5581 accgcagtct taacgaacgg cggcgccgta tcttaccgcg cgggcctcaa tggccctggg
     5641 gtggatctgt gcggtggagg gtatcttgcc ggagatgttc tctatcctgt ccgcggtggc
     5701 gggatgtgtc gccgtgatgc cgccccccga cgatccgctc ctttgctcga gccgtttgag
     5761 gaaagacagg agggcccggg gatcgtaccc gcacttcgcg agggtctgca cggcggattc
     5821 gtccgccgcg tattcctgtg accggccgta cccgttgacg acgagggtct tgaagacgtc
     5881 atcgatgctg ccctcgaaga ggctgaccag ctgggagacc tcaccggagg tgtagttgcg
     5941 cgccgccgtg gtgccgatga cggtgagcgc ctccgtccag cgcgcgctct tgatcgcggc
     6001 tatgccgtcg cgcttgctta tgtgcgcggc ctcatgggcg agtaccgcgg ccagctcatc
     6061 ctcgcttgcc gtcgtccgta tcatgccgct cgtgatgaag atagttccgc ccggggcagc
     6121 gaaggcgttt atctcgttcg tctccaggat ggcgaaatgg tacccaccgt aggtataggg
     6181 tttgtcggaa ttgagggcta ccgtccttcc gatgagattt acgtagcgcg tgagctgcgc
     6241 gttcgttgtc aattgatagg acccgagtat ttttgcggcc acggcgcggc cgacatagta
     6301 ttcctcttcg tcggagatgg gacgggcggc cttggacgtc tggtccacca cggtcatcgc
     6361 cgtgccgatg tgctgcgaga tattgccgaa gctgctggtg tcgcatgagg cgagcgctgc
     6421 caggagaaag ggggcgcaca ggagcgcgag gcatttcttt gtcatggctg cctcaacccc
     6481 ccggctacga tgaactgcgc gatgtcccgt tcgggaacag gatagcgctc gacgctgtca
     6541 acgagaccga atttcatttc cggatgctgt ttcttgtatg ttgcctcgac ctgcgggttg
     6601 aatcccttcc cggcgagcgc cgcctcgctc tcgctgacgg aggacccctt tcgcgaatag
     6661 gaggcaggaa cggcgacgac cctcttctcc acggcgctcc tgtggatgta gccgatggag
     6721 gggccgcact tcaccttgta ccagtcgcct tccatggaga gggcctccaa cctgtcgtta
     6781 tatcgcacgt tggccttgac gggcgcgaag aactttgcat actcacggat cgcgttctct
     6841 ttcgtgatga ccgtgaccgt gtcggccagc gcggcggtcg ccgccgc
//
LOCUS       DAOQTR010000034         6845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_12218_length_6845_cov_3.844183, whole genome shotgun sequence.
ACCESSION   DAOQTR010000034 DAOQTR010000000
VERSION     DAOQTR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12218_length_6845_cov_3.844183"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PKN85_01575"
     CDS             <1..1085
                     /locus_tag="PKN85_01575"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT43118.1"
                     /translation="YPSIRQWARDTGKKVVGSTIADVPEEVIYAFDLLPVTILGTHKP
                     LKKAPSHLPDNACSLARSNLELALSYEGDLFSGFVLPQVCDTTQHLSDIWRINFPDKF
                     VVSYLAPRQADRPSARYWVKEEIERLIESLSAWTGKAVNDDALRRSIEIHNETRRLLA
                     ELYDIKRKNPGTITNKQLFSMMKLSLQVDKAEFNKSLRSIKEGIKPKKGKGYTDVVLV
                     GITCDPPEIFDIFDELKLNIVGDTLVDGTRYLQGAPALNGNLIDALADRHFKRGFFSP
                     IHDNVYKNFEEVKKLYKETKAKAVVYVHIEFCESQEYDLPDLKDMIKKEGIPMHVLDT
                     EYQTTSLSHVLTRAQAFFESLKGGSL"
     gene            1082..2320
                     /locus_tag="PKN85_01580"
     CDS             1082..2320
                     /locus_tag="PKN85_01580"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT43119.1"
                     /translation="MSDKLTSKQLSKKITDEYMEDAFHAHDNNKLVGYTTAISPVEIF
                     VAHDIVPIYPENHAVANLTAKKGVELCSVVEGMGYTSHLCAYARSDLGYRKTGVTVTR
                     GIPDPDLFLACNAQCFTLTKWFQVLARRGDLPVFVFDTPEHILDQKARKEIVKYCVLQ
                     LKELISFLEKVTKRKFDYDRLKEVMKYSAASSILYKKFLDMAQYKPSPISIFDALIGM
                     AIAVYRRGTQECVDYYQTLCDEIQAKVDQGIGVLPKDQEKYRLYWENLPVWFKFSDHA
                     KLLGSYGGVILTSLYVHAWSFDFDLNKDPLETLAENYVNRFSNSTIEDRADMAMELFK
                     KYSMNGMIMFMNRSCKAVSFAVPTLKDVLTKRTGIPALVFESDMGDQRFYAESQVRTR
                     IEAYFETLDRLGLAQKTAAA"
     gene            2468..3010
                     /locus_tag="PKN85_01585"
     CDS             2468..3010
                     /locus_tag="PKN85_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNT43120.1"
                     /translation="MDIGKKIREFRDAKSLNIKQLAELVECTPSLISQLERGKADPSI
                     SMLKRIAEALNVNIVDFFMKRANDDDVVTRKDSRIVIQLNRWDARIQSLVRNTADKKM
                     QPFYTVIKPGGGSHGMYSHDGEEFGYVVQGEMDLMLDNKLHKIHKDESFYFSSKVPHD
                     WGNSGKEDVIVIWVITPPTF"
     gene            3039..4529
                     /locus_tag="PKN85_01590"
     CDS             3039..4529
                     /locus_tag="PKN85_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT43121.1"
                     /translation="MPLLKEVSRNYGKLDLHINGKWVKPKTGKYFEATNPATGKVIAQ
                     APVAGPEDVEKAIVAAHAAFGKWRNVPFRDRAKLIFNLRDTFMKHHEELARILVQDHG
                     CTIEDGRGTISRCIENIESAGSSMYSFYKGEHVEQLANGIDCYLIHEPVGVFLIITPG
                     NIPMHAWSSFVPYALACGCTVIVKPSRQCPVAADAMSKMVDEAGFPPGVVNLLHMGPE
                     RELNRVILSDPRVQGVGLIGSTRVSKELFEICGQYGKRSSLNGNGKNTIVIMPDANLD
                     QSVNYILRGCFGMSGQRCLGSDNVVVLGDNKLYKTLKDKLIAASKAMKMGYGLDESVE
                     LGPMTTPGGREQVIGFVESGLKSGAKLLLDGRKAKVKGYEEGYFLGPNIFENVTPDMH
                     IAKEEAFGPLCNLLRMDSLDETIGFINSTNYGHSACIVTESGKSARKFIRECEVGNVG
                     VNAGIPQPYAFFGLGSKKDSFFGNSKSRMDSVKMFLDEKTVTLRWV"
     gene            4634..5716
                     /locus_tag="PKN85_01595"
     CDS             4634..5716
                     /locus_tag="PKN85_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT43122.1"
                     /translation="MCEECKKISRRTFLKGAAVVGAAAGLGVFDSKLLHAQSANLKFS
                     TWHPPVSREVKTVWTPMMEEVKKRSGGKLAYTMYAGAALGKGPEHYDIVAKGLSDMGY
                     FTATWTPGRFPLTDVLSLAVWVDGKDTAADIGNAVYHRVLKDEFKDVKVLELNGCIQS
                     FIWTKKPISKLSDLKGVKLRSPGGHQTNYIKSLGAEPVFMPLGDVYMGMETGTVDGIV
                     TCAPLVLAFKLAEVARYGTVLTFGCVSEGTVMNMKSWNRLPADQKKIVEDVCTNPFKV
                     TGGLGKDDYKKIMADIQKAGVKMIDLPKAEQELWYKRFQDVTRKWVSDMEAKKLNARR
                     AVAVMNEECEKRNVKLVSCPPEFKKV"
     gene            5738..6229
                     /locus_tag="PKN85_01600"
     CDS             5738..6229
                     /locus_tag="PKN85_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT43123.1"
                     /translation="MKALEGFQKGVHRVTYAVCAVGMFLAIPLMLITTFDVLGRAIFN
                     KPLPGTLELSEYMLSIIILLGAAYTQQVKGHVAVDFLTSRFPVKGQLVCKIITDILCL
                     FVITIMTVYGWIEGISETTVTDQLRVPMWPFKTLVAVCGLMLWAEFLTDLITSIMSLG
                     RRK"
     gene            6229..>6845
                     /locus_tag="PKN85_01605"
     CDS             6229..>6845
                     /locus_tag="PKN85_01605"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT43124.1"
                     /translation="MDPVTVGILGCVLLVILLFLGMPIAFVMMFVGFTGLWALSSLDA
                     ALPKLAETVWGVANNYPYTIIPLFILMGSFAGSGGITRELYNTFDKWARRLPGGLGIA
                     TIAACAAFGAVSGSSVAAAAAMGNVALPEMRRFGYDPKLGCGVIAAGGTLSFLIPPSL
                     GFVVYGMLTEQSIGKLLISGILPGALMAAAFIACVVTLVGINPKLA"
BASE COUNT         1645 a         1991 c         1914 g         1295 t
ORIGIN      
        1 cgtatccttc catccgacaa tgggctagag atacaggaaa gaaagtagtt gggagtacca
       61 tcgccgatgt tcctgaagag gtgatctatg cgttcgatct cctccccgtg actattcttg
      121 gtactcataa acccttaaag aaggcgccca gtcatcttcc ggacaatgcc tgttcgctgg
      181 ctcggagcaa tctcgaactc gcgctgagtt acgagggcga tcttttcagc ggttttgttc
      241 tgccccaggt ctgtgacacc acacagcacc tttcggacat atggcggatc aattttcccg
      301 acaagttcgt cgtgagctac ctggcccccc gccaggcaga caggccgagc gcccggtact
      361 gggtcaagga ggagatcgag cgtctcatcg aatccctcag cgcgtggacg ggcaaggcgg
      421 taaatgacga cgcgctcagg aggtccatcg agatacacaa cgagaccagg agactccttg
      481 ccgaacttta cgacatcaag aggaagaacc cgggaacaat aacgaacaaa cagctctttt
      541 ccatgatgaa actctccctg caggtcgaca aggccgaatt caacaagagc ctgaggtcga
      601 tcaaggaggg tatcaagccg aagaagggca aggggtacac cgacgtggtg ctcgttggaa
      661 taacctgcga tccgcccgag atattcgaca tcttcgacga actcaaactg aacatcgtcg
      721 gagacacgct cgtcgacggc acccgctacc ttcagggcgc cccggcactg aacggcaacc
      781 tgatcgacgc gctggcggac aggcatttca agcggggttt cttctcaccc atccacgaca
      841 acgtctacaa gaacttcgaa gaggtcaaga aactctacaa ggaaacgaag gcgaaggcgg
      901 tcgtctacgt gcacatcgaa ttctgcgaat cccaggaata tgatctgccc gacctcaagg
      961 acatgatcaa gaaggaaggc atcccgatgc acgtccttga cacggaatac cagacgacgt
     1021 ccttgtccca cgtcctgaca cgggcccagg cattcttcga atctctcaaa gggggatcat
     1081 tatgagcgat aagctgacat cgaaacaact gtccaaaaag atcaccgacg aatacatgga
     1141 agacgccttc catgctcacg ataacaacaa gctcgtcggg tacacgacgg cgatctcccc
     1201 cgtggagatc ttcgtcgccc acgacatcgt gcccatctat cccgagaacc acgccgtcgc
     1261 caacctcacg gccaagaagg gtgtggaact ctgctccgtc gtcgaaggca tgggctacac
     1321 gagccacctg tgcgcctacg cccggagcga cctgggctac cgcaagacgg gcgtcaccgt
     1381 gacaaggggc attccggacc ccgatctttt cctcgcctgc aacgcccagt gcttcaccct
     1441 gaccaagtgg ttccaggtgc ttgcccgcag gggcgacctc cccgtcttcg tcttcgacac
     1501 accggaacat attctcgacc agaaggcgcg caaggagatc gtgaaatact gcgtcctcca
     1561 gctcaaggaa ctcatcagtt tccttgaaaa ggtaacgaag aggaaattcg attacgaccg
     1621 cctcaaagag gtgatgaaat actccgctgc gtccagcatc ctttacaaga agttcctcga
     1681 catggcccag tacaaaccgt cgcccatcag catcttcgac gccctcatcg gcatggccat
     1741 cgccgtctac cgcaggggta cccaggaatg cgtcgactac taccagacgc tctgcgatga
     1801 gatccaggcg aaagtggacc agggcatcgg cgtcctgccg aaggaccagg aaaaataccg
     1861 tctctactgg gagaacctgc ccgtctggtt caagttcagc gaccacgcga agctgctcgg
     1921 ctcgtatggc ggcgtcatcc tcacctcgct ctacgtccac gcctggagct tcgacttcga
     1981 cctcaacaag gacccgctgg agacactcgc ggagaactac gtgaaccgct tctccaactc
     2041 caccatcgag gacagggccg atatggccat ggagcttttc aagaaatact ccatgaacgg
     2101 catgatcatg ttcatgaaca ggagctgcaa ggccgtctcc ttcgcggtcc ccaccctgaa
     2161 ggacgtcctg acgaagagaa cgggcatccc cgccctcgtc ttcgagagcg acatgggcga
     2221 ccagcgcttc tacgccgagt cacaggtccg gaccaggatc gaggcatatt tcgagaccct
     2281 cgacaggctg ggtctcgcgc agaagaccgc ggcggcataa gcaggtcttc ggcacctccg
     2341 ttaaacaaca acgccccgga cagcggctcc tgtccggggt ttttctttgc tccccaaaaa
     2401 gaccttgaca gaattaaatg tcatttaata aaataaatat tatttaactc atctcatgga
     2461 cagcgacatg gatatcggaa aaaagataag ggaattcagg gacgcgaaga gcctcaacat
     2521 caagcagctt gcggaactcg tggaatgcac cccctcgctg atctcccagc ttgaacgcgg
     2581 caaggcggat ccctcgatct ccatgctcaa gaggatcgcc gaggccctca acgtgaacat
     2641 cgtcgatttc ttcatgaaaa gggccaacga cgatgatgtc gtgacccgaa aagactcgcg
     2701 catcgtcatt caattgaaca gatgggacgc caggatacag tccctcgtga ggaatacggc
     2761 cgacaagaag atgcagcctt tctacaccgt catcaagccc ggcggcggct cccacggcat
     2821 gtattcacac gacggagaag agttcggcta cgtggtccag ggagaaatgg accttatgct
     2881 ggataacaaa ctgcacaaga tccataagga cgagagtttc tacttttcct ccaaggtccc
     2941 ccacgactgg gggaacagcg gaaaggaaga tgtcatcgtc atatgggtca taacgccacc
     3001 tacgttctag gagtggccaa tctttcgagg aggaatcaat gccattgctc aaggaagtca
     3061 gcagaaatta tgggaaactg gacctgcaca taaatgggaa gtgggtcaaa ccaaagacag
     3121 gcaagtattt cgaagccacc aaccccgcga cgggcaaggt gatcgcccag gcccccgtgg
     3181 cgggccctga agatgtcgaa aaggccatag tcgcggctca tgccgcattc gggaaatgga
     3241 ggaacgttcc atttcgtgac cgggcaaagc ttatcttcaa cctccgtgac acctttatga
     3301 agcaccacga ggaactggca aggatcctcg tacaggacca cgggtgcacc atcgaagacg
     3361 gcaggggcac catctcaagg tgcatcgaga acatcgaatc ggcgggcagt tccatgtaca
     3421 gcttctacaa gggcgaacac gtcgagcagc ttgccaacgg gatagattgc tacctgatcc
     3481 atgagcccgt cggtgtcttc ctcatcatca cccccggcaa catccccatg cacgcctggt
     3541 cgtcattcgt cccctacgcc ctcgcctgcg gctgcacggt catcgtaaaa ccgagccgcc
     3601 agtgcccggt cgccgctgac gccatgtcca agatggtcga cgaagcaggg tttccccccg
     3661 gtgtcgtcaa ccttctccac atgggaccgg agcgcgagct gaacagggtc atcctctccg
     3721 accccagggt gcaaggggtg ggactcatcg gctcgacgcg tgtgagcaag gagcttttcg
     3781 agatatgcgg ccagtacggc aagcgttcgt cactgaacgg caacggcaag aacaccatcg
     3841 tcatcatgcc cgacgccaat cttgaccagt cggtcaatta catcctcagg ggctgctttg
     3901 gcatgagcgg ccagcgctgt ctcggctccg acaacgttgt ggtcctcgga gacaacaagc
     3961 tctacaagac cctgaaggac aagctgatcg ccgcctcaaa ggccatgaag atgggctacg
     4021 gcctcgacga gagcgtggaa ctcggaccga tgacaacgcc gggcggcaga gaacaggtga
     4081 tcggcttcgt cgaatcgggc ctcaagtcgg gggcgaagct tctccttgac ggccgcaagg
     4141 cgaaggtgaa aggatatgaa gagggatact tcctcggccc caatatcttc gaaaacgtca
     4201 cccccgatat gcacatagcc aaagaagagg cgttcgggcc gctctgcaat ctcctgagaa
     4261 tggacagcct cgacgagacc atcgggttca taaactcgac aaactacggc cacagcgcat
     4321 gcatagtgac ggagagcggc aagtcggcgc ggaagttcat acgggaatgc gaggtcggca
     4381 atgtgggagt caacgccggc ataccccagc cctacgcctt cttcggcctc ggctccaaga
     4441 aggattcctt cttcggcaac tcaaaatcga ggatggattc tgtgaaaatg ttccttgacg
     4501 aaaagacggt aaccttaaga tgggtttgat cttcacgggc ccgacgcccg ggggtaggaa
     4561 tatcggtacg ttgtaaggta aagcgaggtt tcattgtcag ccacaggaca aaaacccaaa
     4621 aggaggagat ccgatgtgcg aagagtgtaa gaagatttca aggagaactt ttctaaaggg
     4681 ggccgcggtc gtcggggcgg cggcagggtt gggcgtgttc gacagcaagc tcctgcacgc
     4741 gcagagcgct aatctcaagt tcagcacctg gcacccgccg gtgagccgtg aggtcaagac
     4801 ggtctggacg ccgatgatgg aagaggtgaa gaagagaagc ggcgggaaac tggcctacac
     4861 gatgtacgcc ggcgccgcgc tgggcaaagg ccccgagcac tacgacatcg tggcgaaggg
     4921 attgtccgac atgggctact tcacagccac ctggacaccg ggacggttcc cgctgaccga
     4981 cgtcctttcc ctcgcggtct gggtcgacgg caaggacacc gccgcggaca tcggcaacgc
     5041 ggtctaccac agggtgctca aggacgagtt caaggatgtc aaggtcctcg agctcaacgg
     5101 ctgcatccag tcctttatct ggacgaaaaa acccatctcg aagctctccg acctcaaggg
     5161 cgtgaaactg aggtcgccgg gtgggcacca gacgaactac atcaagtctc tcggcgccga
     5221 gccggtcttc atgccgctcg gtgatgtgta catggggatg gagacgggca ccgtcgacgg
     5281 catcgtcacc tgcgcgccgc tcgtgctcgc gttcaagctc gccgaagtcg cgaggtacgg
     5341 caccgtgctc acattcgggt gtgtctccga aggcaccgtc atgaacatga aatcctggaa
     5401 caggctgccg gccgaccaga aaaagatcgt cgaagacgtc tgcacgaacc ccttcaaggt
     5461 gacgggtggt ctcggcaagg atgactacaa gaagataatg gcagacatcc agaaggccgg
     5521 cgtcaagatg atcgacctcc ccaaagcgga acaggagctg tggtacaaga ggttccagga
     5581 cgtgacccgc aaatgggttt ccgacatgga ggcaaagaaa ctgaacgcca ggagggccgt
     5641 cgcggtcatg aacgaagagt gtgaaaagag gaacgtcaag ctcgtttcct gtccgcccga
     5701 attcaagaag gtctgataga agaggctaca gaatgccatg aaagcgctgg aaggttttca
     5761 aaaaggggtg cacagggtga catacgcggt atgtgccgtg ggcatgtttc ttgccatccc
     5821 cctcatgctg atcaccactt ttgacgtgct cgggagggcc atcttcaaca agcccctccc
     5881 gggcaccctt gaactctcgg aatacatgct gtccatcatc attctgctgg gtgccgcgta
     5941 cacccagcag gtcaagggcc atgtggccgt tgacttcctt acgtcgagat ttcccgtgaa
     6001 agggcagctg gtatgcaaga tcataaccga catactgtgt cttttcgtca ttaccatcat
     6061 gacggtatac ggctggatcg agggaatctc cgaaacaacg gtgacggacc agctcagggt
     6121 gcccatgtgg cccttcaaga cactcgttgc cgtgtgcggg ctcatgctct gggcggagtt
     6181 tctcaccgac ctgataacaa gtattatgag cctgggaagg aggaaataat ggatcccgtt
     6241 acggttggta tattaggttg cgtgctcctg gtgatcctcc tctttctcgg catgcccatc
     6301 gcgttcgtca tgatgttcgt gggtttcacc gggctctggg ccttgtcctc actggacgcg
     6361 gccctgccga agctggcgga aaccgtctgg ggcgtcgcca acaactaccc ctacaccatc
     6421 atcccccttt tcatcctcat gggaagcttc gcgggctccg gcgggataac gagggaactc
     6481 tacaacacct ttgacaagtg ggcccgcagg ctgcccggcg gactcgggat agcgaccatc
     6541 gccgcctgcg ccgcctttgg ggcggtgagc gggtcttccg tcgccgccgc cgccgccatg
     6601 gggaacgttg ccctgcccga gatgcgaagg ttcggatacg accccaagct cggctgcggg
     6661 gtgatcgcgg caggcggcac cttaagcttt ctcataccac ccagcctggg gttcgtcgtc
     6721 tacggcatgc ttacggagca gtccatcgga aagctcctta tatcggggat actgccgggg
     6781 gctctcatgg ccgcggcgtt cattgcctgc gtggtaacgc tggtaggcat aaacccgaag
     6841 ctcgc
//
LOCUS       DAOQTR010000035         6771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_12392_length_6771_cov_2.891900, whole genome shotgun sequence.
ACCESSION   DAOQTR010000035 DAOQTR010000000
VERSION     DAOQTR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12392_length_6771_cov_2.891900"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKN85_01610"
     CDS             complement(<1..525)
                     /locus_tag="PKN85_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43125.1"
                     /translation="MAEKIKVLIVDDSAVVRQVLEEVFQSDGALQVVGSARDPFVAAD
                     IMRRVVPDVITLDVEMPRMDGITFLHKIMSQRPIPVVMCSSLTEKNSETAMKALEYGA
                     VDIIEKPRMGVKQYLEESKVIICDTVKAAAEARLGRAKKNNGFIKVEPKLTADAVIEK
                     PNSRAMIQTTERVVV"
     gene            complement(548..1462)
                     /locus_tag="PKN85_01615"
     CDS             complement(548..1462)
                     /locus_tag="PKN85_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNT43126.1"
                     /translation="MALPEDSRSRNADYGSIRLSNRLFEELTVFIRDQVGIKITPVKK
                     VMLEGRLQKRLRKLGMRSFDEYCKYLFSDEGRGGELTSMIDEVTTNKTDFFREPAHFN
                     YLTSRVLPTLLRGGGFTVSNRLTIWSAGCSSGEEPYTIAMVVQDFADTAGAERLPFHV
                     VATDISRRVLEKGQKAVYEDDKVAPIPVAIKKKFLLKSRNPEAGLFRIAPEIRSRVSF
                     RRLNFMDGDFGFREKIDIIFCRNVIIYFDKIVQERLLNKFCRCLKPDGYIFMGHSETL
                     FGMDLPLEQVAPTVYRKTGTIATIRKTA"
     gene            complement(1475..1840)
                     /locus_tag="PKN85_01620"
     CDS             complement(1475..1840)
                     /locus_tag="PKN85_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43127.1"
                     /translation="MTKRIMTVDDSVSVRQMVSFTLKNAGYETVEASDGQDALSKLGG
                     SGIHMIVTDLNMPNLDGIGLIKEVRNKAEYKFIPIIMLTTESQDSKKQEGKSAGATGW
                     IVKPFKPDQLLAVVKKVLG"
     gene            complement(1893..3710)
                     /locus_tag="PKN85_01625"
     CDS             complement(1893..3710)
                     /locus_tag="PKN85_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT43128.1"
                     /translation="MDDIRSGIEGLAARVGRLIPGTEEQFLFIGSRLNEVYVEVEKIS
                     RQASSLVSMLDSSAMKDTIGRFHEILERMDRHIGGSDTRFAAGMSALNDVLSAVSHVG
                     NPLANFRKIVKTLKILSISTKIESAQLKKMESDFVNLAGDVDQLSALIRDKSAGIESH
                     RRSLMSLTEATLARIVTINEKSRGYISEVLGNIRSSIALLESRQDLSFSAADSVLNRF
                     NATSKQVGEVVMSMQFHDITRQQVEHIRDVLTSMEGKLRASSQGEGRGGPDVGPVAPE
                     LVAEAAIACELQKAQLSDAQTKFVHAVDAIVENLRGVVKNVFRILEDIQKITGGTDLI
                     ASTFLSNIESGTTAVIGKLEDTGRAEGEFVDAMGELSSAVSTISGLVDDIEEIGEEIE
                     LIAVNARVKSAHTGVEGAPLGVIAEAIWHLSIDATSQKTTISELLREVVTATEGLQAA
                     VREGTGDGGSDSGSIIGELSGLLNELKGMNDGVVGLVKEIEGGSRSLSGLIEETIESI
                     SVHKDLRREVSGLSALFDEVIAAARRAVSHEELEKARGLSLEYIASNYTMQRERIIHQ
                     SVASNVIPFTGRDRGDVTPPAPEEGGACDDDLGDNVELF"
     gene            complement(3971..4444)
                     /locus_tag="PKN85_01630"
     CDS             complement(3971..4444)
                     /locus_tag="PKN85_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNT43129.1"
                     /translation="MDVKYINPFIMAAQSVFRTMLGIEVTLSKPVIKTSRTTSGEVTG
                     IMGLVGDRKGTITISFQDKGALFIYKTLIGDESTAINSDVVDAIGEITNIISGQARKE
                     FEKAGINLKAAIPMVIVGKEIELNFITTLPIVQLPFNFSVGNGTQEVMFLDFSFE"
     gene            complement(4464..4838)
                     /locus_tag="PKN85_01635"
     CDS             complement(4464..4838)
                     /locus_tag="PKN85_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43130.1"
                     /translation="MKVLVVDDFATMRKIIKNVLKQINIENVLEAENGKHALTVLGGE
                     EVDLIISDWIMPEMTGIEFLKACKGDDNIKKIPFIMVTAEAQKDNIMEAIKSGVDNYI
                     VKPFTPEKLREAIDKAKAKVGK"
     gene            5050..6432
                     /locus_tag="PKN85_01640"
     CDS             5050..6432
                     /locus_tag="PKN85_01640"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNT43131.1"
                     /translation="MTEQDFLTTLENYGQHHILEHYRALTPHQRGVFLEASRGLDIRL
                     VMKLHRQFDQAECASPVPTGIRPPRVVCVPRTSREQNGALRARSIGESLLQRQKVAVL
                     VVAGGQGSRLGFDGPKGTFPLSPVKRKTLFQLFAEQVRYLSVRFGVTIPFLIMTSTDN
                     HEDTVQFFQANRHFGLAPDSVHFFSQGVLPAVSPDGRLILRDNVHFYTSPNGHGGSLK
                     ALSDSGLLAKLIAAGYEDLFYCQVDNPLVRIADPVFLGHHSSEAAQVSTKVVRRRSVD
                     EKVGVYLDVNGRQAVIEYSDLDESHMQALDEKGEILYWAGNTAIHIFSLSFIRDLNTD
                     GFALPYHCARKSAYVVSSDWEPASIDVWKFETFVFDAIPLAERACCMEVAREEEFAPV
                     KNKEGPDSPDTARQALIELHRAWLKKAGVEVPPDAKVEVSPLFAFTPEEAAEKLRTRK
                     VVASPEIYIE"
     gene            complement(6526..>6771)
                     /locus_tag="PKN85_01645"
     CDS             complement(6526..>6771)
                     /locus_tag="PKN85_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HNT43132.1"
                     /translation="VVALSPDASVVRRMKLYRGVIPYQVRKLASTDRMIGEVDRFMVE
                     AGFAKEGDVIVLVAGHPIATKSRTNFIKLHRVGEKTV"
BASE COUNT         1327 a         2072 c         1774 g         1598 t
ORIGIN      
        1 gacgacgaca cgctccgttg tctggatcat ggcgcgggag ttgggcttct cgataacggc
       61 atcagcggtg agcttcggtt ccacctttat gaaaccgttg ttcttcttcg cgcgcccgag
      121 gcgtgcctcg gctgccgcct tcaccgtatc gcagatgatc accttcgatt cctcgaggta
      181 ttgtttcaca cccatccggg gcttctctat gatgtccacc gccccatact ccagcgcctt
      241 catggccgtc tcggaattct tttccgtgag gctcgagcac atgacaacag ggatgggcct
      301 ctgggacatg atcttgtgaa ggaaggtgat gccgtccatc cgcggcattt ccacatcgag
      361 cgtgatgaca tcagggacga cacgtctcat gatgtccgcg gcaacaaagg ggtccctggc
      421 ggagccgacc acctgcagcg ctccgtcgga ttggaaaacc tcttcaagca cctgtctcac
      481 cactgctgaa tcgtcaacga tcaggacttt gatcttctct gccatcatgc ctccgcttac
      541 gccctgttca tgctgttttt ctaatggtgg ctattgtgcc ggtcttcctg tacaccgtcg
      601 gcgctacctg ttcgagggga aggtccatgc cgaagagcgt ctcggaatgg cccatgaaga
      661 tgtacccatc aggcttcaga caccggcaga acttattgag aagcctttcc tgaacgatct
      721 tgtcgaaata gatgatcaca ttcctgcaga agatgatgtc tatcttttcc cggaagccga
      781 aatcgccatc catgaaattg agcctcctga aggagacccg ggaccgtatc tcgggggcta
      841 tcctgaagag ccccgcctcg gggttcctgc tctttaacag aaacttcttc ttgatcgcca
      901 ccggtatggg agccaccttg tcatcctcgt agacggcttt ctggcctttc tccagaacac
      961 gccgggaaat atcggtcgcg accacgtgaa aaggcaggcg ctccgcgccc gctgtatccg
     1021 cgaagtcctg caccaccatg gcgatggtgt agggctcttc gccactcgaa cagcctgcgc
     1081 tccagatggt cagcctgttc gagacggtga atcccccgcc cctgaggagc gtgggcagga
     1141 ccctcgaggt gaggtagttg aagtgagcgg gttcgcggaa gaaatccgtc ttgttcgtcg
     1201 tcacctcgtc gatcatgctc gtgagctcgc cgcctcgccc ctcgtcgctg aacaggtact
     1261 tgcaatactc gtcgaacgat ctcatcccca gcttgcgcag cctcttctga agtcttccct
     1321 cgagcatgac cttcttgacg ggggtgatct tgatccccac ctggtcgcga atgaagacgg
     1381 tcagttcctc gaagagcctg ttcgacagcc tgatggagcc gtagtcggcg ttccggcttc
     1441 gcgaatcctc agggagcgcc atcaccctta cctcctatcc caggactttc ttcacgaccg
     1501 ccaggagctg gtccggcttg aaaggtttca ctatccaacc agtggcgccg gcgctcttgc
     1561 cttcctgctt cttgctgtcc tgcgattccg tcgtcagcat gatgatcggt atgaacttgt
     1621 actcggcctt gtttcgaact tccttgatga gtcctatccc gtcaaggttc ggcatgttga
     1681 ggtcggtcac gatcatatgg atccccgagc cgcccagctt cgacagtgcg tcctgtccgt
     1741 cgcttgcctc aaccgtctca taccccgcgt tcttgagggt gaaactgacc atctggcgga
     1801 cgcttacaga atcatcgaca gtcatgatac gtttcgtcac cgcactcctc cttgtggatc
     1861 aaaatgcatg tatagattca cggtgttcat catcaaaaga gctccacgtt gtcaccaaga
     1921 tcatcgtcgc aggcaccacc ctcttccggg gccgggggcg ttacgtctcc cctatccctt
     1981 cccgtaaagg gaataacatt cgatgccacg ctctggtgta tgatcctctc cctctgcatc
     2041 gtgtaattgc tggcgatgta ctccagggaa aggccgcggg ccttctcgag ttcctcatgg
     2101 gacacggcgc gtcttgccgc ggcgatgacc tcatcgaaga gcgccgagag gcccgacacc
     2161 tcacgtctga ggtctttgtg gacggagatg ctctcgatcg tttcctcgat gaggccggac
     2221 aggcttctgc tgcccccctc tatctccttc accagaccga cgaccccgtc gttcatgccc
     2281 ttgagctcgt tgagcagccc cgacagttcg ccgataatgc tgccgctgtc agagccgccg
     2341 tcgccggtcc cctctcgcac ggcggcctga agtccctctg tggccgtcac gacctccctg
     2401 aggagctcgg atatggtcgt tttctgtgac gtggcatcta tggaaaggtg ccatatcgcc
     2461 tccgctatga ccccgagcgg tgcgccctca accccggtgt gcgcggactt gacgcgggca
     2521 ttgacggcga tgagctctat ctcctcgcct atctcttcta tatcgtccac gaggccggag
     2581 atcgtcgaca cggcggaaga gagctctccc atggcatcga cgaattcacc ttccgctcgt
     2641 cccgtgtcct caagtttccc gatgacggcc gtggttcccg attcgatatt ggacagaaag
     2701 gtgctcgcga tgaggtcggt tccaccggtg atcttctgga tgtcttccag tatcctgaaa
     2761 acattcttca cgacaccccg gaggttctcg actatggcat cgacggcatg gacaaatttg
     2821 gtctgggcgt cggaaagctg ggccttctgc agctcgcagg cgatcgcggc ctccgccacg
     2881 agctcgggag cgacaggccc gacgtcgggt ccgccacggc cctctccctg tgatgacgct
     2941 cggagtttgc cttccataga ggtcaggacg tccctgatat gctccacctg ttgacgggta
     3001 atatcgtgga actgcatgga catgacgacc tctccgacct gcttcgatgt cgcgttgaat
     3061 cggttcagca cggaatcggc ggcactgaac gagaggtcct gcctgctctc gagcagcgcg
     3121 atgctcgatc gtatgttgcc gagcacctcg cttatgtagc ccctgctctt ctcgttgatc
     3181 gttacgatcc tggcgagagt tgcctcggtc agggacatga gggagcgccg gtgactctcg
     3241 atccccgccg acttgtcgcg gatgagtgcc gaaagctggt ccacatcgcc cgcgagattg
     3301 acgaagtcgc tctccatctt cttcagctgg gcgctctcta tcttcgtgga tatggagagt
     3361 atcttcagcg ttttcacgat cttgcgaaaa ttggcaagag ggttgccgac gtgggagacc
     3421 gcggagagaa cgtcgttaag agcggacatg cctgccgcga acctggtgtc ggagcccccg
     3481 atgtgcctgt ccatcctctc gagtatctcg tgaaagcggc ctatggtatc tttcatggcg
     3541 gagctgtcga gcatgctcac cagtgatgag gcctgacgcg atatcttctc gacctccacg
     3601 tacacttcat tgaggcggga acctatgaaa aggaactgtt cctccgtgcc tggtatcagc
     3661 cggcccaccc gggccgccag tccttcgatc ccacttctga tgtcatccat atatcccgac
     3721 ctttcctcgc ttcatcggtt cgttccttcg agggtaaacc tctgacgctt ccatactgta
     3781 gattgtcgct gttccggggg ataacttaag gaggcaaaac cggttccagg gttcaaaagt
     3841 tcaagagttc aagggggaaa agaccgcttg gaaccaccag gtgccggggc aagagaacag
     3901 ggttcaggag gggtccttac catctcctga accctgcggg ttgtctcttc ctgctgcctg
     3961 tcccgtccct ttattcgaaa gaaaagtcca ggaacattac ttcctgggtg ccgttgccta
     4021 cggagaaatt gaagggcaac tggactatgg gcagggtggt gatgaaattg agctcgatct
     4081 ccttgcccac aatgaccatc gggatggccg ccttgaggtt gatcccggcc ttttcgaatt
     4141 ccttccgtgc ctgccccgat attatgttcg tgatctcgcc gatggcatcg accacatctg
     4201 aattgatggc cgtcgattcg tctcctatga gggtcttgta tataaaaaga gcccccttgt
     4261 cctggaagct tatcgtgatc gtacccttcc tgtcccccac gagacccatt atgcccgtta
     4321 cctcgcccga ggtcgtacga ctcgtcttga tcaccggttt gctcaacgtt acctcaatgc
     4381 ccagcatcgt cctgaatacc gactgtgccg ccataatgaa agggtttatg tactttacat
     4441 ccatgcctgc ctccggcgct gtattattta ccgaccttgg ctttcgcctt gtctatcgct
     4501 tccctgagct tctctggggt gaagggcttc acgatgtagt tgtccacacc cgatttgatc
     4561 gcctccatta tgttgtcctt ctgggcctcc gcggtcacca tgataaaggg gatcttctta
     4621 atgttgtcgt cgcccttgca tgccttcagg aactcgatac ccgtcatctc cggcattatc
     4681 cagtcgctga tgatgaggtc gacctcttcc ccgccgagaa cggtgagggc gtgcttgccg
     4741 ttctcggctt cgagcacatt ttcgatattt atctgcttca gaacattctt gatgatcttc
     4801 ctcatcgtcg cgaaatcgtc aacaacgagc actttcattg ataacctccg ttgcttgttg
     4861 ggtttggctc cattctaaac taattatagt tatcgtgcaa agcggaatta acttaatgta
     4921 acatccgtca ctcagttcaa aagaaaacga ctttcaaggc gaatcccgtt gtcatcggac
     4981 ggccaaatcc tgtaatattt ggaatacggc gaatgatcga aacgcaccgc tcacgcgagg
     5041 attgataaaa tgacagaaca ggacttcttg acgacccttg agaactacgg gcagcatcac
     5101 atccttgaac actaccgtgc tctcacgccc caccaaaggg gtgtattcct cgaggccagc
     5161 cgcggcctcg acatccgcct tgtcatgaag ctccacagac aattcgatca agccgagtgc
     5221 gccagccccg tccccaccgg catcagaccc ccccgtgttg tctgcgtacc ccggacctcc
     5281 cgcgagcaga acggcgccct ccgggcgcgg tcgattgggg aatcattgct gcaacgccag
     5341 aaagtggcgg ttctcgtcgt ggccggcggg cagggttcgc gcctcggttt tgacggcccc
     5401 aagggaactt ttccgctctc gccggtcaaa aggaagactc tcttccagct ttttgccgaa
     5461 caggtccgat atctttcggt gcgttttggc gtgaccatac cctttctgat catgacaagc
     5521 acagacaacc acgaggacac ggtgcaattc ttccaggcca accgtcattt cggtctcgcc
     5581 cccgactcgg tacacttctt ttcccagggg gtcctcccgg ccgtctctcc cgacggcagg
     5641 ctcatcctcc gggacaacgt ccatttctat acgagcccga acggccacgg cggttcgttg
     5701 aaggcgctct ctgacagcgg tcttctggcc aaactcatag cagcgggcta tgaagatctc
     5761 ttctactgcc aggtggataa tcccctcgtc aggatcgccg accctgtgtt tctcggccac
     5821 cattcatcgg aggccgccca ggtatcgacg aaggtagtgc ggcgcagaag cgtcgacgag
     5881 aaggtcgggg tctacctcga cgtcaatgga agacaggctg tcatcgagta cagcgacctt
     5941 gatgagagcc acatgcaggc actcgatgaa aagggcgaga tactctactg ggccgggaac
     6001 acggccatcc acatcttttc cctttccttc atcagggacc tgaacacgga tggttttgcc
     6061 ctgccctatc actgcgccag gaagtcagcc tacgtcgtct cctccgactg ggagccggcc
     6121 agtatcgatg tgtggaagtt cgaaaccttc gtcttcgacg ccataccgct ggccgaacgg
     6181 gcgtgttgca tggaggtcgc ccgtgaggaa gaattcgctc ccgtgaagaa caaggaaggt
     6241 cccgattcac ccgataccgc ccggcaggcc ctcatcgagc tccaccgcgc ctggctcaaa
     6301 aaggcgggcg tcgaggtccc gcccgacgcg aaagtggagg tcagtccgct cttcgccttc
     6361 accccggaag aggcggcgga aaagctgcgg accagaaaag tggtcgcatc accggagata
     6421 tatatagaat aaaggttatg ggtgatgggt gatggaacaa gaccccaaca ctcccacctc
     6481 ctcacaagct ccaacggttc caacgattct aacggctcca acggttcaaa ccgtcttttc
     6541 ccccaccctg tgcagtttga taaagttcgt tctgcttttc gtggctatcg ggtggcctgc
     6601 cacgagaacg atgacatccc cttctttcgc gaaacccgcc tccaccatga acctgtccac
     6661 ctccccgatc atcctgtcag tgcttgccaa tttcctgacc tggtagggga tcacgccgcg
     6721 atagagcttc atccgccgca ccacggaagc gtcgggcgaa agcgccacga c
//
LOCUS       DAOQTR010000036         6613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_12779_length_6613_cov_3.763647, whole genome shotgun sequence.
ACCESSION   DAOQTR010000036 DAOQTR010000000
VERSION     DAOQTR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12779_length_6613_cov_3.763647"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PKN85_01650"
     CDS             complement(<1..873)
                     /locus_tag="PKN85_01650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT43133.1"
                     /translation="MRILIAEDDFTSRAILTAVLQKQGYEVVSTVNGADAWATMQQPD
                     APRLAILDWMMPEMDGIEVCQHIRSLETNQPPYIIMLTSKDEKANIITGLEAGADDYL
                     VKPYDPGELGARVGVGLRMIEMRAALIESRKALAHQATHDPLTGMLNRRAILDRLHKE
                     LAPAAHDGDAVAVAICDIDHFKRVNDTHGHQAGDEVLRAFAQVLIENLREHDSAGRMG
                     GEEFLVISPIRAGMEYTCLFDGLCSRIAASTITTRSGALSVTVSIGAACATAGGTVDE
                     VLGTADAALYQAKNE"
     gene            complement(900..3146)
                     /locus_tag="PKN85_01655"
     CDS             complement(900..3146)
                     /locus_tag="PKN85_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43134.1"
                     /translation="MADRTAKTDRAPEIIEALESRIDRLERELAARDKTVSTLMKRVE
                     RGIDTAGSAFSLFERNTLLEEIVEKRTRELNRALEKLRAAKEAADAASVTKSEFLANM
                     SHEIRTPMNGVMGMTGLLLGTDLNEEQRRYAETAHASAESLLALINDILDFSKIEARK
                     LDLEMLDFDLRALLDDFSAMLALRAQEKGLEFICSAAPDMPTHFRGDPGRLRQILTNL
                     TGNAVKFTNTGEIAVRASLVSETDDEAVVLFSIKDSGIGIPEDKQDILFQKFTQVDAS
                     TTRKYGGTGLGLAISKQLAELMDGEIGVRSKEGRGSEFWFTVHLAKQRERKAPGAPPA
                     EIRGAHILLVDDNATNRRVLMAQLLAWGVRAGEASDGPAALETLRGAKDAGDPFQIAI
                     LDMLMPGMDGTELARLVKADKALKDTHLVLLSSLGQRGDARRMEEIGFSAYLTKPARQ
                     SDLAGCLSAVLAGRATAEQVRPIVTRHMVREMRRSSVRILLAEDNITNQQVALGILKR
                     LGLGADAVTNGREVLKALETLPYDLVLMDVQMPVMDGLEAARQIRDPGSAVRDHHVPI
                     IAMTAHAMQGDRVKCLNAGMNDYLSKPVSPEALARALKKWLPDDAGAATEQNPFEHKV
                     PGPATASGTAASLFDRAGMMARLMGDEDLARTVVKGFLEDLPQQIETLKGCLEAGDAP
                     SAERQAHSIKGASANLGGEALRAVAFEMEKAGRAGDLETARVRLPELMDQFALLKAAM
                     NEFVGSGR"
     gene            complement(3139..4257)
                     /locus_tag="PKN85_01660"
     CDS             complement(3139..4257)
                     /locus_tag="PKN85_01660"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNT43135.1"
                     /translation="MRVRWGRSLREGAEAAVSELYQAIGTSGPDILIFFCSAGYDRTG
                     LSKALEARFRCPIIGCTTAGEISPAGYQQGGIVAAAVTSRQLKVHTRILPSLSRRKAS
                     DFMDLAVDLRKNLTLGTSFDKDHMFGLLLIDGLSKMEDETIASLYSNLDGIPIIGGSA
                     GDDLRFQETFVYANGSFHGDSAVFALCETSLPFSTFKTQHFEPTDLKIVVTEADARSR
                     IIREINGYPAGVEYARVLGLPVEDLNPTVFSENPLMIRLGGDYYVRSIQKVNPDMSLS
                     LFCAIEEGLVLTIGKGSDMAEDLREAIRGLRIDPHGDHLAIFCDCILRKLEAADKSLE
                     EDFGRILSGVTAVGFSTYGEQFNSIHVNQTLTGVIIHG"
     gene            4578..6362
                     /locus_tag="PKN85_01665"
     CDS             4578..6362
                     /locus_tag="PKN85_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43136.1"
                     /translation="MNGLMDAYRGAMEIMEDAGGGIGMQAMPMQPAATPEKEMERRRE
                     IINRQYRQAKSMMGSLPAGTEKPQISEAFPLEGYIIVNGGEKEFFSNEVKTDLLYTIK
                     ERFVGNLVVIHAYDPAKGQFDREKEYDIQSLSTKIDVPGVLGKKCVRRSSYVPSSCTR
                     WARFLRYEISNGERYPQFRAGVVSASPAANGRIKVTADAPSVTFMGDDGTTNLGMGCF
                     SATWTLGVSEFKKLLKGGEVLLRKDIGRPMGPSPGCRPGSTMTLYLKMRESAEECKDM
                     RSVKLMIISPEDKSRYVFSDEYLLSEHSNKLTLELEARTVPEHYADSIEWTVPEIEGS
                     TRTIVPPSTSSTPKGRKLTVVYKGLPEDNNEFGRKTVKASLKVGSCRMEETREVRLFY
                     PRDEKNNPEGKYPNWFYYWKQTPAARPFGQNVRIAYQCEGIPADKCVCGQRGVIGQYN
                     PYYSDYKTINVCDLKTNTWDPDTFYVKMPAVKRSIPSTLSGKSYLDYTYIDTFAVDIM
                     HEFTHFNNFHTFWPEGWKESEDRDGDDIPDRLEPGMGFSPDLKQTYWQGVDLSGDEEF
                     LTLEATYDYKTGTYDEYDWARPGKNWPK"
     gene            complement(6441..>6613)
                     /locus_tag="PKN85_01670"
     CDS             complement(6441..>6613)
                     /locus_tag="PKN85_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT43137.1"
                     /translation="SRAARKGRNPRTGKEIKIAAKKVPRFSAGATLKAVVNGKAVAKP
                     AKKKPAAKPKKK"
BASE COUNT         1316 a         1935 c         1975 g         1387 t
ORIGIN      
        1 ctcgtttttt gcctgataca gggccgcgtc ggcggtgccc agcacctcat ccaccgtccc
       61 tccggcggtt gcgcaggccg cgccgatgct caccgtaacg gacaacgctc ccgatcttgt
      121 cgtgatcgtg ctcgcggcga tccttgaaca caagccgtcg aagaggcatg tgtattccat
      181 gcccgccctt atcggggaga tcaccagaaa ctcctctccc cccatgcgcc cggcggaatc
      241 gtgttcacgg agattttcga tcagaacctg cgcgaatgcg cggagcacct catcgccggc
      301 ctgatggccg tgcgtgtcgt taacgcgctt gaaatggtct atgtcgcaga tcgcgacggc
      361 cacggcatct ccatcatggg ctgccggggc gagttccttg tgcagccggt cgagaatggc
      421 gcggcggttc agcatacccg tcaggggatc gtgggtggcc tggtgtgcca acgctttgcg
      481 gctttcgatg agagcggccc gcatctcgat catgcgaagg ccgaccccga cccgggcgcc
      541 gagttccccg gggtcataag gcttgacaag gtagtcgtcc gctccggctt ccagcccggt
      601 gatgatgttc gccttctcat ccttggacgt cagcattatg atataaggcg gctggttggt
      661 ttcaagggaa cgtatgtgct ggcacacctc gataccgtcc atctccggca tcatccaatc
      721 gaggatcgcg agtctggggg cgtcgggctg ctgcatggtt gcccaggcgt ccgcgccgtt
      781 cacggtcgac accacctcgt agccctgctt ttgaagtacc gccgtcagga tggcgcgtga
      841 tgtgaaatcg tcttcagcga tcaatatccg catggatacg ttcccccgat catggtctgt
      901 catcgtccgg agcccacgaa ttcattcatt gctgccttca gcaaggcgaa ctggtccatc
      961 agttccggca ggcgcaccct ggcggtttcg agatcccctg cccttccggc cttctccatc
     1021 tcaaaggcca cggcgcgcaa agcctcaccg cccagattgg ccgatgcgcc cttgatgctg
     1081 tgggcctggc gctccgcgct cggggcatcg ccggcttcca ggcagccttt cagggtctcg
     1141 atctgctgcg gcaggtcctc aagaaagcct ttcaccaccg ttcgcgccag atcttcatcg
     1201 cccataaggc gtgccatcat ccccgccctg tcgaacaatg acgccgcggt cccgctcgcg
     1261 gtggcgggcc ccgggacttt atgctcaaag gggttttgct ccgtcgctgc cccggcatcg
     1321 tcgggcagcc acttcttgag cgcacgggcc agagcctcgg gggaaacggg cttgctcaaa
     1381 tagtcgttca tccccgcgtt caggcacttc acgcggtcac cctgcatcgc gtgggcggtc
     1441 atggcgatga tcgggacatg gtgatcgcga accgcggatc cgggatcgcg gatctggcgg
     1501 gcagcctcca gtccgtccat tacgggcatc tgaacgtcca tgagcaccag gtcgtagggg
     1561 agggtctcga gggctttcag gacctcccgc ccgttggtca ccgcgtctgc acccagaccc
     1621 agtctcttga gtatccccag tgccacctgc tggttggtga tgttgtcttc ggccagaaga
     1681 atgcgcacag agctccgacg catctcgcgg accatatgac gcgtgactat gggccgcacc
     1741 tgctcagccg tggctcttcc ggccagcacg gctgacaggc agccggcgag gtccgactgc
     1801 cgagccggct tggtcaggta ggcggaaaag ccgatctctt ccattctcct ggcatcaccc
     1861 cgttggccca gagaggaaag aaggaccagg tgagtgtcct tgagcgcttt gtcggccttg
     1921 accagtctgg ccagctctgt gccatccatt ccgggcatca gcatgtcgag aatagctatc
     1981 tggaaaggat cgccggcgtc tttcgccccg cggagcgttt caagggccgc gggcccgtcg
     2041 gaagcctccc cggcacgcac gccccaggcg agaagctggg ccatcaagac ccttcggttg
     2101 gtcgcgttgt catcgaccaa aaggatatgc gccccccgga tctcggcggg aggagcgcca
     2161 ggggctttgc gttccctttg ctttgcgaga tgcacggtga accagaattc cgaaccccgg
     2221 ccctccttgc tcctgacccc tatttcgcca tccatcagct cggccaattg ctttgagatc
     2281 gccagcccca gcccggtgcc gccatacttg cgtgtcgtcg aggcatccac ctgcgtgaac
     2341 ttctggaaca ggatatcctg cttgtcctcg gggatgccga tgccgctgtc ctttatcgag
     2401 aaaagcacca cggcctcatc gtctgtctcg gagacaaggc tcgcccgcac ggcgatctcc
     2461 ccggtgttcg tgaacttcac cgcgttaccc gtgaggttgg tgaggatctg gcgcagacgg
     2521 ccggggtcgc cgcggaaatg ggtcggcatg tccggagcgg cagagcagat gaactcaagc
     2581 cccttctcct gggcgcgcaa ggccagcatg gcggagaagt cgtcaagcag ggcgcgcaga
     2641 tcgaagtcca gcatctccag gtcgagcttg cgcgcttcga tcttggaaaa atccaggata
     2701 tcgtttatga gcgccaggag tgattccgcg ctcgcgtgcg cggtctcagc gtagcgccgc
     2761 tgctcctcgt tgagatcggt gcccagcagg aggcccgtca tgcccatgac accgttcatg
     2821 ggtgtgcgga tctcatggct catgttggcc aggaactcgc tcttggtgac gctggccgca
     2881 tccgcggcct ccttggcggc ccgcaatttc tcaagggccc tgttgagttc tcgtgtccgc
     2941 ttttccacga tctcctcgag aagggtgttg cgctcgaaga gcgagaaggc gctccccgcg
     3001 gtgtcgatac ctcgttccac ccttttcatg agggttgaca ccgtcttgtc ccgggcggca
     3061 agttcccgtt cgagacgatc gatccgcgac tcaagagcct cgatgatctc cggggcacgg
     3121 tcggtcttcg ctgttctgtc agccatggat gatcaccccg gtaagggttt gattgacgtg
     3181 aatggagttg aactgctcgc cgtacgtgct gaatcctacg gcggtgaccc cggagaggat
     3241 gcgtccgaag tcttcttcca gtgacttgtc cgccgcttcc agttttctca atatgcaatc
     3301 gcagaaaatg gcgaggtgat ctccatgggg gtctattcgc agcccacgga tcgcctctct
     3361 cagatcctcc gccatgtccg atccctttcc gatggtgagg acgagaccct cctcgatggc
     3421 gcaaaagaga ctgagactca tgtccgggtt gaccttctga atggaacgga cgtagtagtc
     3481 tccgcccagg cggatcatga ggggattctc cgagaatacg gtgggattga gatcctcgac
     3541 cgggagaccg aggaccctcg cgtattccac ccccgcggga taaccgttga tctccctgat
     3601 gatcctgctc ctggcgtccg cctccgtcac gacgatcttc aggtctgtcg gctcgaaatg
     3661 ctgggtcttg aacgtggaaa aaggcaaaga ggtctcgcac agcgcaaaga cggcgctgtc
     3721 cccgtggaaa gagccgttgg cgtaaacgaa ggtctcctgg aagcggaggt cgtccccggc
     3781 ggacccgccg attatgggta ttccatcaag attggaatag agcgaggcga tcgtctcgtc
     3841 ttccatcttc gacagcccgt cgatcaacag gagtccgaac atgtggtcct tgtcgaagga
     3901 ggttcccaga gtaaggttct ttctcaagtc gacggcaaga tccataaaat cggaggcctt
     3961 cctccgggac agggaaggca ggatgcgcgt gtggaccttc aattgccgcg aagtgacagc
     4021 cgccgcaaca attcctccct gctggtatcc cgccggggag atctcgcctg ccgtcgtgca
     4081 accgataatg ggacatcgga agcgggcctc caacgccttc gaaagtccgg tcctgtcgta
     4141 tcccgcggaa cagaagaata tcagaatatc cggcccggat gtccctatgg cctgatagag
     4201 ctccgatacg gcagcctccg ctccctcccg cagggacctc ccccagcgta cacgcatgcg
     4261 gtcttgctcc gcggcgctct tgttggaatg gttcatattt cttttttatc ggctatgggg
     4321 gaggaggctt tagggatatt tgtgtgaagg gttgtaaata attcctgaag atactattct
     4381 aacttctggg cgtcgactgg tttcaggcgt cagaaaggat gagcatgatg atcggcgtaa
     4441 agggaagaat tttgtggata ggtctgatcg gggtagttgt cttttgcctg ttttgtgtcg
     4501 aagtgcttgc tcagtctcca ggggatctcg agcgaagggc acgacagctt gagcaacaac
     4561 tgcagggtac gactgacatg aacgggctta tggacgcgta tcggggggcc atggagatca
     4621 tggaagacgc cggcgggggt atcggtatgc aggccatgcc catgcagccg gctgcaaccc
     4681 cggaaaaaga aatggaacgc cgtcgcgaga tcatcaacag gcagtaccga caggcaaaga
     4741 gcatgatggg cagtcttccg gcaggcacag agaagccgca gatctccgaa gcttttcccc
     4801 tggagggata catcatcgtc aacgggggag agaaggagtt cttctccaat gaagtgaaga
     4861 cggatcttct ctacacgatc aaggagaggt ttgtcggcaa cctggtcgtc atacatgcct
     4921 acgatccggc aaaaggacaa tttgacaggg aaaaggagta cgatatccag tctctgtcaa
     4981 cgaagatcga cgtgccgggc gttctgggaa agaaatgcgt gcgcaggagt tcgtacgtgc
     5041 cgtcatcctg tactcgctgg gcccgctttc tgcgctacga gatctccaac ggggagcgct
     5101 acccgcaatt tcgtgccggc gtggtgagcg cgagccctgc cgcgaatggc cggatcaagg
     5161 tcacggccga tgcgccttca gtcaccttta tgggggatga cggtacgacg aacctgggga
     5221 tggggtgttt cagtgccaca tggacgctgg gcgtgtcaga gttcaagaaa ctgctcaagg
     5281 ggggcgaggt gctgttgcgc aaggacatcg gcaggccgat ggggccctct cccggttgcc
     5341 gccccggttc caccatgacg ctgtatctca agatgaggga gagcgcggag gagtgcaagg
     5401 acatgaggtc cgtgaagctc atgatcataa gccctgaaga caagagcagg tatgtcttca
     5461 gcgatgagta cctcttgagc gagcattcca acaagctcac tttggaactt gaggcgagaa
     5521 cggtgcccga gcactatgcg gattccatcg agtggaccgt tcccgagata gaaggctcca
     5581 cgcgaaccat tgttccccct tcgacatcat cgacgccgaa aggacgaaaa ctcaccgtcg
     5641 tctacaaggg actgcccgaa gacaacaacg agttcggcag gaagaccgtg aaagctagcc
     5701 tgaaagtggg ttcctgcagg atggaggaaa cgagggaggt gcgcctcttc tatccgaggg
     5761 atgagaagaa caatcccgag ggcaagtatc ccaactggtt ctattactgg aaacagaccc
     5821 cggcggcaag gccgttcgga cagaatgtca ggatcgcgta ccagtgcgaa gggattccgg
     5881 ccgataaatg cgtttgtggc caacgcggcg ttataggcca atataatcca tattattccg
     5941 actacaagac cattaacgtg tgcgacctca agacgaacac gtgggaccct gacacgttct
     6001 atgtgaaaat gccggcggtc aaacgatcaa taccgtcgac gctttccggg aagagttacc
     6061 tcgactacac gtacattgac acctttgccg tcgatattat gcacgagttc acccacttca
     6121 ataatttcca caccttttgg cccgaaggct ggaaagagag cgaggatcgc gacggggatg
     6181 atattcctga caggcttgag ccgggaatgg ggtttagtcc ggacttaaaa cagacgtact
     6241 ggcagggtgt cgacctgagc ggcgacgagg agttcctgac attggaggcc acctacgact
     6301 acaaaacggg gacctatgac gagtacgact gggcgagacc gggcaagaac tggcccaaat
     6361 agatactaag gcctgaagaa cagcacgaag cgcgtccgaa cgggaaggaa aagcgggccc
     6421 gaaggcccgc ttcgcttgat ctatttcttt ttcggcttgg ctgccggttt cttcttcgcc
     6481 ggttttgcaa ccgccttgcc gttgaccaca gccttcagcg ttgctcccgc ggagaacctt
     6541 ggaaccttct tcgcggctat cttgatctcc tttcctgtcc gggggtttct tcccttccgg
     6601 gccgcacggc tgg
//
LOCUS       DAOQTR010000037         6603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_12806_length_6603_cov_4.312156, whole genome shotgun sequence.
ACCESSION   DAOQTR010000037 DAOQTR010000000
VERSION     DAOQTR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12806_length_6603_cov_4.312156"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1512)
                     /locus_tag="PKN85_01675"
     CDS             complement(328..1512)
                     /locus_tag="PKN85_01675"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT43138.1"
                     /translation="MGISKAISKSMRDSSWIRAMFEEGEKMKKIHGPENILDFTLGNP
                     VMEPPARLKEELVRLVTSDDPGMHRYMTNSGYEDVRTEIAEFHSQRTGLPFTRDHIVM
                     TVGSAGGMNVVLKSLLDPGNEVIVLTPFFVEFKFYIENHGGVMKLVPTTEDFHLDVAK
                     IEAAITRKTKAIIINSPNNPTGVVYGEKELQDLAAVLHKRREKGQRVFLLSDEAYRKI
                     LYDGVVLPDVFNIYEDTISVTSHSKDLALPGERIGYITASPRITNLKPLMDAMIFSNR
                     ILGFINAPALMQRLVSRFQKNSVDILDYQKKRDALYNILVEAGLSVVKPMGAFYIFPR
                     SPIPDDIAFVRELQKHHILAVPGVGFGTPGHFRLAYCIPMDVIERSRKHFLELGLKNG
                     RK"
     gene            complement(1519..2709)
                     /locus_tag="PKN85_01680"
     CDS             complement(1519..2709)
                     /locus_tag="PKN85_01680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT43139.1"
                     /translation="MIVSRQIQKLISDQEGWTRRMFEEGIKIKKLYGEENVYDFSLGN
                     PDTEPPRELQEMIARALLDPVKGMHRYMTNSGYEEVRQEVAGYLEETYSLPFTASHVF
                     MTNGSAGGLNILLKSMLNRGDEIIVPTPFFWEFKNYVVNHGGKLKTVRSKDDFQLDVD
                     SIAAAITPRTRAVLLNSPNNPTGAVYSQKSISDLVALLNGKRSQGQDIYLIADDAYRK
                     LVYDGITLPNLFKAYDLTFSINSHSKDLALPGERIGYIAISPRMSDAGLLVSGLMITQ
                     RTLGFVNAPALFQRVVAKFQRTSVDIGEYQRKRDVLYDALMEGGFECVKPEGAFYMFP
                     RSPIADELTFVRMLQQDERIMVVPGRGFGKKGYFRIAYCVPMETIEKSAQGFIRIGKR
                     FMKR"
     gene            3128..3979
                     /locus_tag="PKN85_01685"
     CDS             3128..3979
                     /locus_tag="PKN85_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT43140.1"
                     /translation="MAGGSQGYSGSDDYIASKALMEIVNVAVILKKPLVVKGEPGTGK
                     TLLAHSIARALDKKLLIWNIKSTTKARDGLYVYDTVQRLNDARFKDKDISDIRQYIKM
                     GKLGQSFSSDEQVILLIDEIDKADIEFPNDLLNELDEMSFHINELDEEVTAKHRPIVI
                     ITSNSEKELPDAFLRRCLFYYIEFPDGEMMEKIIKVHYPDIEDKLVREALKKFYWIRE
                     MDGLKKRPSTSELLDWIKALALGGIKIEKINNEIPFLGTLLKNEQDTDRFVRVSTNQG
                     GFGFRRR"
     gene            3982..5199
                     /locus_tag="PKN85_01690"
     CDS             3982..5199
                     /locus_tag="PKN85_01690"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT43141.1"
                     /translation="MFIDFFFMLRRKGVPVSIKEWMSFIEALYEGHFQSNLNHLYHIG
                     RAFLVKSEAYYDAYDLAFQEFFGGIATEDVELDKVLGWLENPLNRLPKMSPEEIMEFQ
                     RQLEEFKRTHDLEELMRQFRERLKEQKERHDGGSKWVGTGGKSPFGAYGYHPGGIRVG
                     GESWMQSASKVATERRFRNYRNDIILDVRQTKMALKKLRELKREGTEEELDIEETIDK
                     TAKEGGEIELVFSKSRENSVRIILLMDTGGSMLPYTELCEKLFSAATQANHFKEFRYF
                     FFHNCIYQDIYEDMANYKNVPTEKLFSLFNKNYKLILVGDARMAYSELFEVNGCIDYF
                     STNDKPGLEWLSRIRDHFPHSVWLNPTHKNFWGHYTVDTIGKLFPMYELTIDGLKDAI
                     KALTSKTRNLLHQ"
     gene            5388..>6603
                     /locus_tag="PKN85_01695"
     CDS             5388..>6603
                     /locus_tag="PKN85_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT43142.1"
                     /translation="MSAAMDRVSSLKNTELFRALEDGDLEKLSTKLRERVYPPNTAIV
                     REGASGDAMFIIKNGQVEVRKREQNLGVDLTIATLGMGACFGEMALLTGKPRSATVMA
                     IAATELFVLEKKDFDALLLEHPSISISINKIVAERIEEMNTQKSMGVVSMQTLKIDPD
                     FITVIPEAEALKYKMLATSYGNGSLTIAMVNPQDILALDEARRLIKAKFTKSVALDQV
                     IVTEEDFKHFMEGEYKTITKPLVEEKPEVNLDDFLDSMDHIQSDIMKDVEFDEGQEDD
                     SGITDLAREAEGAPIIRLTNNIIAMALKKGASDIHIEPMERGLRVRYRIDGVLREEMM
                     LPKKVQLPLISRVKIISKLDITERRLPQDGRITIKLGSKSVDFRVSTVPTKFGEKICT
                     RILDKTNTTMGLD"
BASE COUNT         1511 a         1834 c         1866 g         1392 t
ORIGIN      
        1 actgcagcgt attagaagac tgagaagatc ttatctcacg cccgttcgtc gcgttcgctc
       61 ctcatagccc tattatcctg aaaaagtatc cacctctcat ccccccctgt agtcataggt
      121 cctatagtct cctcaaaccc catactccct cacccccccg cactcccatc cctccacctg
      181 aaaagcatcc tctgctttcc ctcccgtcgg agacgggatg caaagacggc cagactccgg
      241 cgggcgccgg aatctggccg aatcttttct ctgtgtgctc tcctcagaaa ctccgtgcac
      301 tcgagcgaag cgggcgtgag atgtttctta tttcctcccg ttcttgagtc ccagttcgag
      361 gaagtgtttc ctcgatcgct ctatcacatc catggggata caataggcga ggcggaagtg
      421 gcccggggtg ccgaagccga cgccggggac agcgaggatg tggtgcttct gtagttccct
      481 cacaaaggcg atgtcgtcgg ggatgggcga cctgggaaag atgtagaagg cacccatggg
      541 tttgacgact gagagaccgg cctcgacgag gatgttgtag agcgcgtcac gtttcttctg
      601 gtagtcgagg atgtcgacgc tgttcttctg gaacctgctc acgaggcgct gcatgagggc
      661 gggggcgttg atgaatccca gtatcctgtt cgagaagatc atggcgtcca tgaggggttt
      721 caggttcgtg atgcggggag atgcggttat gtagccgatg cgttcgccgg gcagggcgag
      781 gtccttggag tgggaggtca ccgatatcgt gtcctcgtag atgttgaaga cgtcgggaag
      841 gacgacgccg tcataaagta ttttcctgta agcctcgtcg gaaaggagga agacgcgctg
      901 tcccttttcc ctgcgcttgt gaaggacggc cgccaggtct tgaagctcct tctcgccgta
      961 gacgaccccg gtgggattgt tcggggagtt gatgatgatg gccttggtct ttctcgttat
     1021 ggcggcctcg atctttgcga catcgaggtg gaaatcctcc gtcgtcggca cgagcttcat
     1081 cacgccgcca tggttttcga tgtagaactt gaactccacg aagaaggggg tgagcacaat
     1141 gacctcgttg ccgggatcga ggagggactt gaggacaacg ttcatcccgc ccgccgaccc
     1201 cacggtcatg acgatgtggt ccctggtgaa gggcaatccc gtcctttgac tgtggaactc
     1261 ggcgatctcg gtgcgcacgt cctcgtagcc gctgttcgtc atgtagcggt gcatgcccgg
     1321 gtcgtcggag gtgacaaggc gcacaagctc ctctttcaac cgggcggggg gctccatcac
     1381 ggggtttccg agtgtgaagt cgagtatgtt ctcgggtccg tgtatcttct tcatcttctc
     1441 gccttcctcg aacattgccc gtatccagga agaatctctc atcgatttgc tgatagcttt
     1501 cgatataccc atttttgttc acctcttcat gaaacgtttt cctattctga tgaatccctg
     1561 cgcactcttc tcgatcgtct ccatggggac gcagtacgcg atcctgaaat aacccttctt
     1621 tccgaaaccc cttccgggca cgaccatgat ccgctcatcc tgctgcagca tcctgacgaa
     1681 ggtgagttcg tcggcgatgg gagagcgggg gaacatatag aacgccccct caggtttcac
     1741 gcattcaaag ccgccctcca tcagcgcgtc gtagagaacg tccctctttc gctgatattc
     1801 gccgatatct accgacgtgc gctggaactt cgcgacgacc cgctggaaca gggccggggc
     1861 gttgacgaag cccagggtcc tctgcgtgat catcaggccg gacacgagga ggcccgcgtc
     1921 ggacattcga ggagagatgg cgatgtagcc gatccgctcc ccgggcaggg cgaggtcctt
     1981 cgaatgggag ttgatggaga acgtcaggtc gtaggctttg aagagattgg gcagggttat
     2041 gccgtcatag acaagcttcc ggtatgcgtc atctgctata aggtatatgt cctgtccctg
     2101 ggagcgcttc ccgttgagga gcgcgacgag gtcggagatg ctcttctgcg aatacacggc
     2161 ccccgtcggg ttgttcgggc tgttgaggag gaccgccctc gttctggggg tgatggccgc
     2221 ggctatgctg tcgacgtcaa gctggaaatc atccttcgac ctgacggtct tgagtttgcc
     2281 cccgtggttc acgacgtagt tcttgaactc ccagaagaag ggcgtcggaa cgatgatctc
     2341 gtcgcccctg ttgagcatgc tcttgagaag gatgttgaga ccgccggcgc ttccgttggt
     2401 catgaagacg tgggaggcgg tgaagggaag gctgtaggtc tcctcgaggt acccggcgac
     2461 ctcctggcgc acttcctcgt aaccgctgtt cgtcatgtag cggtgcatgc ccttcacagg
     2521 atcgaggagt gccctggcga tcatctcctg cagctcccgc ggaggctccg tatcgggatt
     2581 tcccagcgag aagtcgtaga cgttctcttc gccatagagc ttctttatct tgataccttc
     2641 ctcgaacatc ctccgcgtcc acccttcctg gtcgcttatc agtttttgga tctgtcgcga
     2701 aacgatcatc ggctctcctt ttcataaaaa aagccatggg tcccttcggc ccatggctcc
     2761 caagaaaaaa gggccatggg tctttgcacc catggccctt tgctgtctag cgcaaagcta
     2821 tgcccgggtg caagctccta aagaagaaaa aataaaagaa agctgtaaat agcttcatca
     2881 tttgaacatt aaagatcacc atgaggtttt tgtcaaggaa aagatagagg caacaggacc
     2941 cgtctcacct ccgtccatcg gccttcctgg tccctgatcc ccgcctgcaa ccttttcttc
     3001 cgggtatacc ccggggtggt aagtatgccc acacccaact acttcgccga ccggcttacg
     3061 cattgacatc ggcctgctga gaccttataa tatagcggaa tttttaactt tcgtaaggag
     3121 gccacacatg gcaggcggtt cacagggata cagcggaagc gacgattaca tagcatcgaa
     3181 agcactcatg gagattgtca acgtagcggt gattctcaag aaaccgctcg tagtgaaagg
     3241 cgagccgggc accggaaaga cactcctcgc ccacagcatt gcccgggcgc tcgacaagaa
     3301 gctcctgatc tggaacataa agtcgaccac caaggcccgc gacggccttt acgtgtacga
     3361 taccgtgcag aggctcaacg acgcgcggtt caaggataag gacatcagcg atatcaggca
     3421 gtacatcaag atgggaaaat tgggccagtc gttctcgagc gacgagcagg tcatcctcct
     3481 catcgacgag atcgacaaag ccgatatcga gtttcccaac gacctgctca acgagctcga
     3541 cgagatgagc tttcacatca acgagctcga cgaggaggtg acggcgaagc acaggcccat
     3601 cgtcatcata acgagcaact cggagaaaga acttcccgac gcgtttctca gaagatgcct
     3661 cttctattac atcgaattcc ctgatgggga gatgatggag aagatcatca aggtccatta
     3721 tcctgacatc gaggacaagc ttgtcagaga ggccctgaag aagttctact ggatacggga
     3781 gatggacggg ttgaaaaaga ggccgtcgac aagcgagctc ctcgactgga taaaggccct
     3841 cgccctcggc ggcatcaaga tcgagaagat caacaacgag atcccctttc tcgggacact
     3901 cctcaagaac gaacaggata cggacaggtt cgtgcgtgtc tcgacgaatc agggcggatt
     3961 tggattcaga agaaggtaac catgttcata gattttttct tcatgctccg gaggaagggc
     4021 gtccctgttt ccatcaagga atggatgtct ttcatcgagg cattgtacga aggccatttt
     4081 caatcgaacc ttaaccacct ctatcacatc ggcagggcct ttctcgtcaa gagcgaggca
     4141 tactacgacg cctatgacct tgcctttcag gagttcttcg gcggcatcgc caccgaggat
     4201 gtcgaactcg acaaggtgct cggatggctc gagaacccgc tcaaccgcct gccgaagatg
     4261 agccccgagg agatcatgga gtttcagagg cagcttgagg agttcaagcg cacccacgat
     4321 ctcgaggaac tgatgcggca attccgggag cgtctgaaag agcagaaaga gcgccacgac
     4381 ggcgggagca aatgggtcgg cacgggaggc aagtctccct ttggcgccta cgggtatcat
     4441 ccgggcggga tccgggtcgg gggagagtcg tggatgcagt cggcctccaa ggtggccacg
     4501 gagcgcaggt tccgcaatta ccgcaacgac atcatcctcg atgtgcggca gacgaagatg
     4561 gccctcaaga agttgcgcga gctcaagcgc gagggcaccg aggaagagct ggacatagaa
     4621 gagaccatcg acaagacggc gaaagaaggc ggcgagatcg agctggtctt cagcaagtcc
     4681 cgggagaact cggtgcgcat catccttctc atggacaccg gcggatcgat gctgccctac
     4741 acggagctgt gcgagaagct cttttccgcg gcaacgcagg cgaaccactt caaggagttt
     4801 cgctacttct tcttccacaa ctgcatatat caggatatct acgaagatat ggccaactac
     4861 aagaatgtgc cgacggagaa gctcttctcc ctcttcaaca agaactacaa gctcatactc
     4921 gtcggcgacg ccaggatggc ctattcggaa ctctttgagg tcaacggctg catcgactat
     4981 ttctcgacga acgacaagcc cggcctcgag tggctttcaa ggatacgcga tcatttcccg
     5041 cactcggtat ggctcaaccc gacacacaag aacttctggg gacactatac tgtcgacacc
     5101 atcggcaagc tcttccccat gtacgagctt accatcgacg gcctgaagga cgcgataaag
     5161 gcattgacat cgaagacgag gaaccttctc catcagtagg atatcgtcgt gtcaaaggcc
     5221 gctcgcccgg gtagtggcgc gggcaaaaat aactattact atttttgaga atatccttta
     5281 taattttcag caggccggcc tcaagccggc ctcaaagact taactacccc ggtggagctt
     5341 aagggacaga tgaacgatcg gggcccaaca ccctggagga tgtcgtagtg agcgccgcaa
     5401 tggacagggt ttcgtcgctc aagaacacgg aactgttccg tgcccttgag gatggtgatc
     5461 tggaaaagct ttcgacgaaa cttcgggaaa gggtctatcc tccgaacacg gccatcgtcc
     5521 gtgaaggcgc ctccggcgac gcgatgttca tcatcaagaa tggacaggtc gaggtaagga
     5581 aaagggagca gaacctcggt gtcgatctca ccatagcgac gctcggcatg ggggcgtgct
     5641 tcggcgagat ggcgctcttg accggcaagc cccgctcggc cactgtcatg gcgatcgcgg
     5701 cgacggagct ttttgtcctc gagaagaagg atttcgacgc gctgctcctc gagcatccca
     5761 gcatctccat atccatcaac aagatagtcg cggaacgcat cgaggagatg aacacgcaga
     5821 agagcatggg cgtcgtctcc atgcagacgc tcaagatcga tcccgatttc atcaccgtca
     5881 tacccgaggc ggaggcattg aagtacaaga tgctggccac ctcgtatggc aacgggagtc
     5941 tgaccatcgc catggtcaat ccgcaggaca tactcgccct cgacgaggcg cgcaggctga
     6001 tcaaggcgaa gttcacgaag agcgttgccc tcgatcaggt catcgtcacc gaggaagact
     6061 tcaagcattt catggagggc gagtacaaga ccatcacgaa gcccctcgtc gaggaaaagc
     6121 cggaggtcaa cctcgacgat ttcctggatt ccatggacca catccaatcg gacataatga
     6181 aggatgtcga gttcgacgaa ggccaggagg acgactcggg tatcaccgat ctcgcgcgcg
     6241 aggccgaggg cgcccccatc atcaggctga cgaacaacat catcgccatg gccctgaaaa
     6301 agggtgccag cgatatccac atcgaaccca tggagcgcgg gctgcgcgtg cggtaccgca
     6361 tcgacggcgt gctgcgcgag gagatgatgc tcccgaagaa ggtacagttg cctctcatca
     6421 gcagggtgaa gatcatatcg aaactcgaca tcacagagag gcggcttccc caggacggca
     6481 ggatcacgat caaactcggc agcaagtccg tcgatttccg tgtctccacc gtgccgacga
     6541 agttcggaga gaagatctgt acaaggatcc tcgacaagac gaacacgaca atgggcctcg
     6601 aca
//
LOCUS       DAOQTR010000038         6582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_12871_length_6582_cov_3.555079, whole genome shotgun sequence.
ACCESSION   DAOQTR010000038 DAOQTR010000000
VERSION     DAOQTR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12871_length_6582_cov_3.555079"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1358
                     /gene="bioB"
                     /locus_tag="PKN85_01700"
     CDS             372..1358
                     /gene="bioB"
                     /locus_tag="PKN85_01700"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNT43143.1"
                     /translation="MTSLFDTLADRAIATRDISVKDALELYEAGKQDPFGLMARGSRI
                     REHFKGKTVNLCGIVNAKSGVCPENCRFCAQSAHYHTDAPVYPLISAEDIAEKGRAAK
                     ESGAHMFSIVTSGTRIETEAEWETIYRAIAMLSEMGLKPCGSLGMLSEETAGNLKKAG
                     LYRYHHNLETSRSFFDSICSTHEYDEDTATVRIARAAGLTVCSGGIIGLGEAMEHRIE
                     MAMTLRELAVDSVPINILNAIPGTPLENLPALSPMEILLTVALYRFILPDKDIKLCGG
                     KEKNLRQLLPLAMVAGCNSLLTGNYLTTLGRDTSADLEMIHDLGMVAKFDEA"
     gene            complement(1848..3053)
                     /locus_tag="PKN85_01705"
     CDS             complement(1848..3053)
                     /locus_tag="PKN85_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT43144.1"
                     /translation="MPQFQWEGRTSSGETRRGVSKASSGDALRAALRKEGVILTSMQE
                     TKEAGKQKYNPQKKIKNIEILLFTRQLSTMITSGLPLVQSLEILGNQIEDVDFRGIVK
                     EIKEKIEGGARFADALRDYPKCFDELFINLVVAGEEGGLLDNVLQRLAIYIEKTEKLK
                     KKVKSAMIYPVAIIVVAFVVVVVLLLFVIPVFEKMFKEMGAELPVPTQIVINLSQMMQ
                     SYWYIIIGGVVGLVLALKYYYKTEGGHRNIDRIILKLPLFGLLTIKASVARVTRTLAT
                     LLMSGVAILESMIIVAKVAGNKIVEEALLVARSRISEGRTMAEPLEQAGIFPPMVVQM
                     VQVGESTGALDSMLNKIADFYEEDVDNMVTNLTAMMEPMIMVFLGVVLGGLIIAMYLP
                     IFKLGQAVG"
     gene            complement(3490..4956)
                     /locus_tag="PKN85_01710"
     CDS             complement(3490..4956)
                     /locus_tag="PKN85_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNT43145.1"
                     /translation="MKNKRLVVTVCVLFAAALAFAYVRAQVLPEARPAAPQVQNVRAV
                     AAFNGSGLPSLAGLVNEAKPSIVNISTTAVVRGPGGQGPMMGPNNPFKDFFGDDFFDK
                     FFGNGPRREYKQRSLGSGFIIDKEGYILTNNHVVEKATTIKVKLSDEKEYDARIIGRD
                     PKTDIALIKVDVRQSLPVAVLGDSEVLQVGDWVLAIGNPFGLEHTVTAGIVSAKGRII
                     GAGPYDQFIQTDASINPGNSGGPLLNLKGEVVGINTAIVSGGQGIGFAIPINVAKDML
                     SQLKSKGKVARGWLGVVIQRMTPEIAKSFGLKESEGALVADVMDQSPADKAGIRRGDV
                     IISYNGKKIKDNETLPRLVAATEIGTKAKIILVRDNKQMEVSVTIGELQEEGLRASKK
                     TEIEKDLGLVVQDITAEIAKHLNLKDRKGVIVTDVIPGSPAQEADIRSGDVIREIGRK
                     PVRSVAEFKEAVKRSNVKEGIVMLIQRENATFYAVIRE"
     gene            complement(4970..6376)
                     /locus_tag="PKN85_01715"
     CDS             complement(4970..6376)
                     /locus_tag="PKN85_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43146.1"
                     /translation="MKSFSLPIKWKLTLWYGGILALILVTFSSGVYIYFQNSLQKSID
                     TKIKSIAEVLASSMTETHGQSLFGNFERYLENALGKKPKGKFIQIIDASGKIGARLND
                     LEAEAVPVSFVTLERALKGETVYETLESARPRLRMITMPILDSKKVTSIVQVGSSLEE
                     FEDTMKRLLIIMIISIITSISATIVVGYFMAKKTMKPVDQIRRAAVKISSTNLDERID
                     IKKGRKDELGRLAETFNAMISRLKDSFQRINQFSIDVSHELKTPLTILKGETELALRK
                     ERTNQEYRRSMQSNLEEIDRMSRIIDDLLLLSKAETKDIGMNLEKVDLRDLLAGVCLN
                     MKLFGENKGVEVVVRDLEDIRIVGDELKLRRMITNVVENGIKYGHPGGHVTVSSYKQN
                     GFACINVEDDGPGISGEDIKYIFDRFYRADRSRKRESGSGLGLSISKWIAEAHKGTIE
                     VESKPAAGSLFTIKLPMS"
     gene            complement(6363..>6582)
                     /locus_tag="PKN85_01720"
     CDS             complement(6363..>6582)
                     /locus_tag="PKN85_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNT43147.1"
                     /translation="EFLIMEYMMRNSERPLARKEMAEYAWQSQDDSSNIVDVYVNFLR
                     KKIELLTARKFIHTVRGVGYILKEDEKL"
BASE COUNT         1386 a         1878 c         1795 g         1523 t
ORIGIN      
        1 ccctcatccg ctacctcacc accgagaaca ggaacttcca gcccatgaac ataaacttcg
       61 gccttatcga gggctattcg aaaaagaaca aagaggcaac ggtcgacagg gcgctgcagg
      121 aaatagagaa atggaaaaat agttttaagt gataaaaaca gttttaagtt ttaggtttta
      181 agtgttaagt taagaaaaac agttttaagt gagaagaacg gttttaagtt ttaagtaaaa
      241 gactgaaaag aaagagacga gatagcggag tgctccggca ttactctcac cgcataaagg
      301 aaccgattct ccctgccttt aacttaaaac ctaaaactta acacctaaaa cggtctctga
      361 taggagctat tatgacttcc ctttttgaca ctctggccga tcgcgcgatc gcgaccagag
      421 atatatctgt gaaagacgcc ctggaactct acgaagcagg aaagcaagac cctttcggcc
      481 tgatggcccg ggggtcccgg atacgggagc acttcaaggg caagaccgta aacctctgcg
      541 ggatcgtgaa cgcgaagtcc ggcgtctgcc ccgagaactg ccgtttctgc gcccagtcgg
      601 cgcactacca taccgatgcg cccgtctacc ccctcatatc cgctgaggac atcgcagaga
      661 aagggcgggc cgcgaaggag agcggcgccc acatgttcag catcgtcacc agcggaacga
      721 ggatagagac ggaagccgag tgggagacca tctaccgcgc catagcgatg ctctccgaga
      781 tggggctcaa accctgcggc tcccttggaa tgctgagcga ggaaaccgcc ggaaacctca
      841 agaaggccgg cctctaccgc taccaccaca acctcgagac atcgcgaagc ttcttcgaca
      901 gcatctgttc gacccatgaa tacgacgaag acaccgccac ggtgcgcatc gcccgggctg
      961 ccggcctcac cgtctgctcc ggcggcatca tcggcctcgg cgaggccatg gagcaccgca
     1021 tcgagatggc catgaccctc cgggaactcg ccgtggattc cgttcccatc aacatcctca
     1081 acgccatccc cggcacgccc ctcgagaacc tccccgcgct gagcccgatg gagatcctcc
     1141 tcaccgtcgc cctttaccgc ttcatcctcc ccgacaagga cataaaactc tgcggcggca
     1201 aggaaaagaa tttaagacaa cttctcccgc ttgccatggt ggctggctgc aactcgctgc
     1261 tcaccggcaa ctacctcaca accctcggaa gggacacctc cgccgacctg gaaatgatcc
     1321 acgacctggg gatggtcgca aagttcgacg aagcctgaaa acagaaaagc atcacgcccg
     1381 ttcgtcgcgt tcgctcctca ctcgagcaca cagaggttgt gaggagagca cacagagaaa
     1441 agattcggct atatcccggc accagccggg atatagccgt ctttgcatcc cgcttcgcgg
     1501 gagggaaaaa gaaacttttg gtttcaggca ggagtaatgg gggagaggat aagaaggtgt
     1561 ggggttgtgg tataggggtt ggaggagaga gcataggacc tataggaccc ataggaccta
     1621 tgactacagg gggggatgag ggaagacagg ttgtttaagg ataaggatat tctctttctc
     1681 tcccgccgtg acagcgggac gagaaggccg ccggatcttg gtaggctccg agatccggcg
     1741 gcaatctttt ctctctttta tcctctcttt gcggtctctg cggactcgag agaggagctt
     1801 cagcgacgaa cgggcgtgag atagagtctt tctctttctt cggctgttta cccgaccgcc
     1861 tggccgagtt tgaagatggg gagatacatc gcgatgatga ggccgccgag gacaacgccg
     1921 aggaagacca tgatcatcgg ctccatcatg gcggtaaggt tggtgaccat gttgtccacg
     1981 tcctcttcgt agaaatcggc gatcttgttg agcatgctgt cgagggcgcc cgtcgattca
     2041 ccgacctgga ccatctggac gaccatgggc gggaagatgc ccgcctgctc gagtggttcg
     2101 gccatcgtgc ggccctcgct gatccgggag cgtgccacca gaagggcctc ctcgacgatc
     2161 ttgttgcccg ccaccttcgc gactatgatc atgctctcga ggatcgcgac accgctcatg
     2221 aggagcgtcg ccagggtccg cgtcaccctt gccacggagg cctttatggt gaggagaccg
     2281 aaaagaggga gtttgaggat gatgcggtcg atgttgcggt gaccgccttc cgttttgtag
     2341 tagtatttca gggccaggac gagaccgaca acacccccga tgataatata ccagtaggat
     2401 tgcatcatct ggctcaggtt gatgacgatc tgcgtcggca ccggcagttc cgctcccatc
     2461 tccttgaaca tcttctcgaa aacgggtatg acgaagagaa ggaggacaac gacgacgaca
     2521 aaggccacga ctatgatcgc gactgggtat atcatggccg atttgacctt cttcttaagc
     2581 ttctccgtct tctcgatgta gatggcaagc ctctgcagaa cgttgtccag gagaccaccc
     2641 tcttcgcccg ccacgacgag gttgatgaaa agttcgtcaa aacacttcgg atagtccctc
     2701 agggcgtcgg cgaaacgcgc ccccccttct atcttctcct tgatctcctt gacgataccc
     2761 ctgaaatcga catcctctat ctggttcccg aggatctcca gggactggac aagggggagc
     2821 ccggatgtga tcatcgtgga gagctgccgc gtgaagagca gtatctcgat gttctttatc
     2881 ttcttctggg gattgtactt ctgcttcccc gcctctttcg tttcctgcat ggaggtgagg
     2941 atgacgcctt cctttctcag cgccgcccgc agggcgtcgc cggatgacgc cttcgaaacg
     3001 cccctgcgcg tttcacccga cgaagtcctt ccttcccatt gaaactgcgg catgactcaa
     3061 taatacatga ataaatcccg tttgcaaaga gaaaattatc aaaacaggtg gttaatggag
     3121 aaacgagttc cagaatatga ttcttacact ataaactacg gttataatta aacaaaaatg
     3181 tgacaaacgt cacagagaaa acgggtcatg ggtcacatcc gtccagaatg gatccatggt
     3241 tatggagatg ctgacgctcg ggaacaaacc agggtaacaa aaatgaccaa taatcaaatt
     3301 ccaatgacca atgaaaatga taatgaccaa ttcaccaata accagttgaa cgaagcgaaa
     3361 ggcggactgc ctgtatcgtt tctttcttgg ttattggtta ttgtctgttt ctctggtcat
     3421 tgggatttgg tcattggtca ttatcctctc cggaagaccc acccatcacc catcacctgt
     3481 ctcttctttc tactctctta tcaccgcgta gaacgtcgcg ttctcgcgct ggatcagcat
     3541 gacgatgcct tccttcacgt tggagcgctt gaccgcttct ttgaactccg cgacgcttct
     3601 gacgggcttt ctcccgatct ccctgatgac gtcgccggag cggatgtctg cctcctgggc
     3661 cggtgaaccg gggatgacat cagtgacgat aacgcccttt ctgtccttca ggttgaggtg
     3721 cttcgcgatc tcggcggtaa tgtcctgaac gacgagtccg aggtcctttt cgatctccgt
     3781 ctttttcgat gccctgaggc cctcttcctg cagttcgccg atggtcacgc tcacttccat
     3841 ctgtttgttg tccctgacaa ggatgatctt tgccttcgtg cctatctccg tcgccgcgac
     3901 gagccgggga agggtctcgt tgtccttgat cttcttgccg ttgtaagaga tgatgacgtc
     3961 tccacgcctg atgcccgcct tgtccgcggg gctctggtcc atgacatcgg caacgagggc
     4021 gccctcggat tccttcagcc caaagctctt cgcgatctca ggcgtcatcc tctggatgac
     4081 cacgccgagc cagccgcggg cgaccttgcc cttgcttttc aactgggaca gcatgtcctt
     4141 tgccacattg atgggtatgg caaaaccgat gccctggccg ccggagacaa tggccgtgtt
     4201 tatccccacc acttcccctt tgagattaag gagcggcccg ccggagttgc cgggattaat
     4261 ggaggcatcc gtctggatga actgatcgta cggtcccgct ccgatgatcc tgcccttcgc
     4321 gctcacgatg cctgcggtga cggtgtgttc aagaccgaag ggattgccga tggcgaggac
     4381 ccagtctccc acctggagca cttcggaatc gccaagcacg gcgacgggaa gggactgcct
     4441 gacgtcgacc ttgatgaggg cgatgtccgt cttggggtcc cgccctatta tcctggcgtc
     4501 atattccttc tcgtcggaca gctttacctt gatggtcgtg gccttttcga ccacgtgatt
     4561 gttcgtcagt atgtaccctt ccttgtcgat gatgaatccg gagccgaggc tgcgctgctt
     4621 gtattcgcgc cgtggcccgt taccgaagaa cttgtcgaag aaatcgtcgc caaagaaatc
     4681 cttgaagggg ttgttggggc ccatcatcgg gccctgcccc ccgggccctc tgacgaccgc
     4741 ggtggtgctt atgttgacga tcgacggctt cgcctcattg acgagccccg cgaggctggg
     4801 caacccgctg ccgttgaaag ccgccacggc ccggacgttc tgtacctgcg gtgccgcggg
     4861 cctcgcttcg ggcagcacct gtgccctgac ataggcaaag gccagggccg ccgcgaaaag
     4921 gacacaaacg gttacgacga gtcgtttgtt cttcatggaa tatcctccgt catgacatgg
     4981 gcagtttgat ggtgaacagg ctccctgcgg cgggcttcga ttccacctcg atggtgccct
     5041 tgtgggcctc ggctatccac ttgctgatgg aaagaccgag accgctgccg ctttcccgct
     5101 tgcgcgacct gtccgcgcgg tagaacctgt cgaagatgta ctttatgtcc tcccccgata
     5161 tgccgggacc gtcgtcctcg acattgatgc aggcgaagcc attctgtttg taggatgaga
     5221 ccgtcacgtg cccgccgggg tgtccgtact tgatgccgtt ctccaccacg ttggttatca
     5281 tcctgcgcag tttgagctcg tccccgacga tgcggatgtc ctcgaggtcc ctcacgacca
     5341 cttccacccc tttgttctca ccgaaaagct tcatattgag gcagacaccc gcaagaaggt
     5401 ccctgagatc gaccttctcg aggttcatgc cgatgtcctt cgtttcggcc ttggaaagca
     5461 ggagcaggtc gtcgatgatc cggctcatcc tgtcgatctc ctcgagattg ctctgcatgg
     5521 agcgcctgta ttcctggttc gtcctttcct tcctcagggc cagctccgtt tcgcctttca
     5581 gaatggtaag cggtgtcttg agctcgtggg acacgtcgat gctgaactgg ttgatcctct
     5641 ggaaggagtc cttgagcctc gagatcatgg cgttgaaggt ctccgcgaga cggccgagtt
     5701 cgtcctttcg ccccttcttt atatcgatcc gctcgtcgag gttggtcgag gatatcttga
     5761 cggcggccct gcggatctga tccacgggct tcatggtctt tttcgccatg aagtatccga
     5821 cgactatggt ggcgctgatg gaggtgatga tgctgatgat catgattatg aggagacgct
     5881 tcatcgtgtc ctcgaactct tccagggacg aacccacctg gacgatgctg gtgaccttct
     5941 tggagtccaa tatcggcatc gtgatcatgc gcagccgggg acgggccgac tccagcgttt
     6001 catagacggt ctctcccttg agcgccctct cgagagtgac gaagctgacg ggaacggcct
     6061 cggcctcgag gtcgttcagc ctcgccccga tcttgccgga ggcgtctatg atctggatga
     6121 acttgccctt cggcttcttg ccgagggcgt tctcgagata gcgctcgaaa tttccgaaga
     6181 ggctctggcc gtgggtctcc gtcatcgacg aggcgaggac ctccgcgatc gacttgatct
     6241 tggtgtcgat gcttttctgc aggctgttct ggaaatatat gtagaccccg ctggagaagg
     6301 tgaccagtat cagggccagg atgccgccgt accagagggt cagtttccac ttgatgggga
     6361 gactaaagct tttcatcttc cttgaggatg tagcccaccc cgcgaacggt gtggatgaac
     6421 ttccttgccg tgaggagttc tatcttcttg cggagaaaat tcacgtagac atcgacgatg
     6481 tttgaggaat cgtcctggct ctgccaggca tattcagcca tctccttcct ggcgagcggc
     6541 ctttcgctgt tgcgcatcat gtactccatg atgaggaatt cc
//
LOCUS       DAOQTR010000039         6557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_12925_length_6557_cov_3.589357, whole genome shotgun sequence.
ACCESSION   DAOQTR010000039 DAOQTR010000000
VERSION     DAOQTR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12925_length_6557_cov_3.589357"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PKN85_01725"
     CDS             complement(<1..1199)
                     /locus_tag="PKN85_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT43148.1"
                     /translation="MAQVLAMILAGGRVGELDVLTFYRPKSVLPYGGLYRVIDFPMSN
                     LMHSDIENVGILSQYRPFELISHIANGEPWDMTGRHRRAVILPPFQGRDASDWYKGTA
                     DAVYQNIDFIRMNDPGIVVVLSGDHIYRMDYRKLIEFHLEKNADLTVAFTKVVPEASS
                     RFGLAFIEDEDPRGGRVTQYREKPRNSKLEWASLTIYVFRTELLVAALEENAAHSSHE
                     FGKDIIPMLMENHRVYGYKHSGYWGYTRTIGEYYRTSMDVLGDKPRLDIRSWQLRTNM
                     ADRSIRDRQPAFVGPSASVEDAFFHCGCVIKGRVARSILFPGVTVEEGAVVEDSILFF
                     DSAVRGGAHVKRTIADTGCVISERADVGGAGDDLAVIGMGTVIPEGVRIRGGATVHPK
                     LKADAFS"
     gene            complement(1223..4213)
                     /gene="treY"
                     /locus_tag="PKN85_01730"
     CDS             complement(1223..4213)
                     /gene="treY"
                     /locus_tag="PKN85_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HNT43149.1"
                     /translation="MERPVFRNLRIPLATYRVQFNRDFRFTDAVKVIPYLRELGITDL
                     YASPYFAARPGSLHGYDITDPTRINPEIGTREDYDIFVTTLREHDMGQILDIVPNHMC
                     IDGENRYWMDLLENGPSSPYSGFFDIDWDPVKEELHGKILLPFLGDQYGVVLEDRQIR
                     LEYRDGSFFVLLYEIRLPLLPETYSDILSFGIDHLGERIGETAGYEELLSIITSAGKL
                     PSYVETDPERAAERTREKEIVKKRLNGLYRSDGDIRAFVDRNIAVFNGNEGDPKSFDL
                     LDGLLSRQIYRLSHWQVATEEINYRRFFDINSLAAIRVEDPTVFDETHRFVFELIEGG
                     CVTGLRVDHPDGLYDPSEYFDRLQRRCFAIAMRSHLEEVRENVDLPYGRSYIEPAISE
                     RYDEALRTHRYFKPFYIVAEKILGKGEIMPEEWPLFSTTGYVFLNSLNGLFVDGRNAK
                     AFDTLYRRFTGMQTDFQDVLYENKKLVMEVAMSSEVNTLGRSLNRITEMDRQTRDFTL
                     NSLIKVIIEVIACFPVYRTYINGPDVKERDRHYIELALSRAIRKNPALNESIFLFMKR
                     VLLLEFSADMDEEMRGTWLNFAMKFQQTTGPVMAKGVEDTAFYVYNRLVSLNEVGGSP
                     DRFGTSPDTFHGQNIERIKNWPYALITSSTHDTKRSEDVRARINVLSEIPGEWREHVV
                     LWAKVNRKRKMLVNNRKVPDSNEEYLLYQTLVGSWPFGTVSDDEYESYKGRIKAYMAK
                     AAKEAKVNTSWINPDRMYEEAMTLFVDSILDRRRDNPFLADFVPFQQRVSLFGMYNSA
                     AQTLLKICSPGVPDFYQGTEVWNLSLVDPDNRMPVDHGLRMRMLDEMKEQLSRRDTAA
                     YAKDLTSSMEDGRIKLYITYKALGLRRKMREVFEKGEYIPLEAAGTRSEHVIAFARRF
                     LNTMIIVVVPRFVAKVLSSPGMTFEDMWEDTMVIVPESSEGATFRNVLTGEELARTRR
                     QGLSGLACSGLFRNLPVALLETGR"
     gene            complement(4236..6095)
                     /gene="treZ"
                     /locus_tag="PKN85_01735"
     CDS             complement(4236..6095)
                     /gene="treZ"
                     /locus_tag="PKN85_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HNT43150.1"
                     /translation="MRVFDKTSAPAHLGAVPSADGSTSFRVWAPFARTVSVDMLLGPG
                     GRREPLTPSGGNYFEGKVDGVGDGDLYRYVIDGSSTFPDPASQSQPEGVHGPSRIVDH
                     ASFRWSDDGWRGRALEEYIIYELHVGTFTEQGTFEAIVGCLDYFKDVGINAIELMPVS
                     QFPGSRNWGYDGAFPFAPHNTYGGPRGLKTLVNACHEQGFAVVLDVVYNHLGPEGNYL
                     ANFGPYFTDRHRTPWGEALNFDGPFSDEVRNYFIRNALDWFVRYHVDALRLDAIHGIF
                     DFSARTFLQELSETVGSALSGNPPAYLIAESDLNDIRIITPRENGGIGLDAQWNDDFH
                     HALHTLLTGERQGYYQDFGDIENLAKSFAEGYVYTGEFSSYRVRKHGSPSKHRPATQF
                     IVFSQNHDQAGNRPGGDRLASLVGHDRLKVAATAVLLSPYIPLLFMGEEYGETSPFLY
                     FVDHGDERLIEAVRTGRTREFAWFPSIGEIPDPQREKTFRLSRIDKVSRFEEARRPLF
                     AFYRHLTSLRRSLRPWQMTGGRPLVRCWPDKGTLFVMMPLPGNDVSLFFNFSEENAHV
                     ASPLHEGSWAAVSDTLSTRWGGSGEIAPPRICGKTGTVLSLGPLNAVAYRREG"
     gene            complement(6145..>6557)
                     /locus_tag="PKN85_01740"
     CDS             complement(6145..>6557)
                     /locus_tag="PKN85_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT43151.1"
                     /translation="FASDDPDIHILLLPPFSDRDINALQTIASVILQKSLKEGFGLTV
                     AEGMWKGKPVIGGAAGGIPLQIVHGVTGFLVHSIEGCAFRIRQLLNNTEMAASMGEQA
                     REFVRQNFLITRQVRDYLAVWYTLLTNKKSLVEL"
BASE COUNT         1247 a         1920 c         1981 g         1409 t
ORIGIN      
        1 ttcgagaacg cgtcggcctt gagcttggga tggaccgtgg caccgccccg tatgcggacc
       61 ccttcgggaa tgaccgttcc cataccgatg acggcaaggt cgtcgcccgc gcctccgaca
      121 tcggcccgtt cggatattac gcagccggta tccgctatgg tgcgcttgac atgcgccccc
      181 ccgcggacgg ctgaatcgaa gaagagaatg gaatcctcga cgaccgctcc ctcctcgacg
      241 gtcacgcctg gaaagagaat ggagcgggcc acgcggccct tgatgacgca accgcaatgg
      301 aagaaggcgt cctcgacgct ggccgaagga ccgacgaagg ccggttgccg gtccctgatg
      361 cttctatccg ccatgttggt cctgagctgc caggagcgta tgtccagcct gggtttgtcc
      421 cccaggacat ccatgctcgt ccggtagtat tcgcctatcg tcctggtgta cccccagtaa
      481 ccgctgtgct tgtagccgta aacgcggtgg ttttccatga gcatggggat tatgtcctta
      541 ccgaactcat gggaggagtg tgcggcattc tcctcgaggg cggcgacaag cagttccgtc
      601 cggaagacgt aaatggtaag ggacgcccat tccagtttcg agtttctcgg tttctccctg
      661 tattgagtga cccttcctcc ccgggggtcc tcgtcctcta tgaaggcaag gccgaaacgg
      721 gacgaggctt cggggacgac cttcgtgaag gcgaccgtca ggtccgcgtt cttctccagg
      781 tggaactcta tgagcttgcg atagtccatt cggtagatgt gatctccgga aaggaccacg
      841 acgatcccgg ggtcgttcat ccgtatgaag tcgatgttct ggtacacggc gtcggccgtt
      901 cccttgtacc agtcggaggc gtcccggccc tgaaagggcg ggagtatcac cgccctgcgg
      961 tgccttcccg tcatgtccca gggttcgccg ttcgctatgt ggctgatcaa ttcgaagggc
     1021 ctgtactgcg acaggatacc cacgttctcg atgtcggaat gcatgaggtt gctcatcggg
     1081 aaatcgatga cgcgatacag cccgccgtaa ggcaggacgg atttgggccg gtagaaggtg
     1141 agcacgtcga gttcgcccac cctgcctccc gcgaggatca ttgcgagcac ctgtgccatg
     1201 agacgactcc tttcccggtc gatcaacgac cggtttcgag aagcgcgaca ggaagattcc
     1261 tgaaaagccc ggaacacgca agtcccgata acccctgccg gcgggttcgc gccagttctt
     1321 cgccggtgag gacgtttctg aaggtcgctc cttccgagct ttcgggaacg atcaccatcg
     1381 tatcctccca catgtcctcg aaggtcatgc ccggcgaaga cagcaccttc gccacgaacc
     1441 tcggaacgac cactatgatc atcgtattga ggaaacgacg cgcgaaggct atgacgtgtt
     1501 ccgaccgggt tcctgccgct tccagcggaa tgtattcacc cttctcaaag acctctctca
     1561 tcttccggcg aagaccgagc gccttatacg ttatgtacag ctttattctg ccgtcttcca
     1621 tggaacttgt caaatctttc gcgtatgcgg cggtgtcgcg ccgggagagc tgttccttca
     1681 tctcgtcgag catgcgcatg cgaagcccgt ggtcgacggg catcctgttg tcggggtcga
     1741 cgaggctgag gttccagacc tccgtgccct ggtagaagtc gggtacgccg ggagaacata
     1801 tcttgagaag cgtctgtgcc gcggaattgt acatgccgaa gagggacacg cgctgctgaa
     1861 aggggacgaa gtcagcgaga aaggggttgt ccctccttct gtcgaggatg gaatccacaa
     1921 agagcgtcat cgcctcctca tacatcctgt cagggtttat ccagctggtg ttcacctttg
     1981 cttcctttgc cgccttggcc atgtacgcct tgatccgtcc cttgtatgac tcgtactcat
     2041 cgtcgctcac cgtgccgaaa ggccaactgc cgacgagcgt ctggtagaga aggtactcct
     2101 cgttactgtc aggcaccttc ctgttgttga cgagcatctt tcgtttccgg ttcacctttg
     2161 cccacaggac gacatgctcc ctccattccc cggggatctc ggaaagcacg tttatccttg
     2221 cgcgcacatc ctcgctacgc ttcgtgtcat gggtggagga ggtgatgagg gcgtagggcc
     2281 agttcttgat gcgctcgata ttctgcccgt gaaaggtgtc cggcgaggtg ccgaacctgt
     2341 ccgggctgcc cccgacctcg ttgagggaca cgagccggtt gtacacatag aaggctgtgt
     2401 cctccacacc ctttgccatg acagggccgg tcgtctgctg gaacttcatc gcgaaattga
     2461 gccacgtccc gcgcatctct tcatccatat cggcggagaa ttccaggagc aggacacgtt
     2521 tcatgaagag aaagatcgac tcgttgaggg cgggattttt cctgatggcc cgggacaggg
     2581 cgagttcgat gtaatgacgg tccctttcct tcacgtcggg gccgtttatg taggttctgt
     2641 agaccgggaa gcaggctatg acctcgatga tgaccttgat gaggctgttg agagtgaaat
     2701 cgcgcgtttg ccggtccatc tcggtgatcc tgttcaggct gcgccccagg gtattgacct
     2761 cgctcgacat ggcgacttcc atgacgagct tcttgttctc gtagagcacg tcctggaaat
     2821 cggtctgcat tcccgtgaac cgcctgtaaa gcgtgtcgaa ggccttcgcg ttccgtccgt
     2881 cgacgaagag cccgtttaag gagttcagga acacgtatcc cgtggtgctg aatagaggcc
     2941 actcctccgg catgatctca cccttcccga gtatcttctc tgcaacgatg taaaagggct
     3001 tgaagtatcg atgtgtcctc aacgcctcgt cgtagcgttc cgagatggcg ggttctatat
     3061 aagacctgcc gtaggggaga tcgacgttct ccctgacctc ctcgaggtgc gatctcatgg
     3121 ctatcgcaaa gcagcgccgc tggaggcggt cgaaatactc ggaggggtca tagaggccat
     3181 cggggtggtc caccctcagg cccgtgacac acccgccctc gatgagttcg aaaacaaagc
     3241 ggtgcgtctc atcaaagacc gtcgggtcct cgaccctgat ggcggctagg ctgttgatgt
     3301 cgaagaacct ccggtagttg atctcttccg tcgccacctg ccagtgggac agccggtaga
     3361 tctgccgcga gagcaaaccg tcaagaaggt cgaagctctt cgggtcaccc tcgttgccat
     3421 tgaagaccgc gatgttcctg tccacgaagg cccgtatgtc cccatcgctt cgatagagac
     3481 cgttcagccg tttcttcacg atctctttct cgcgggtccg ctcggctgcc ctttccgggt
     3541 ccgtttcgac gtacgaaggc agttttcctg ccgacgtgat tatgctgagc agttcttcgt
     3601 aaccggccgt ttcgccgatc ctctccccga ggtgatcgat cccgaaggag aggatgtcgc
     3661 tgtacgtctc cggaagaaga ggcagcctga tctcgtagag gaggacgaag aatgagccgt
     3721 ccctgtattc cagccgtatc tgcctgtcct ccagaacgac gccgtactgg tcgccaagaa
     3781 agggcaggag gatcttgcca tgcagctcct ccttgaccgg gtcccagtcg atgtcgaaga
     3841 agccgctgta cggggaggac ggtccgttct ccagaagatc catccagtac cggttctctc
     3901 cgtcgatgca catgtgattg ggaacgatgt cgaggatctg tcccatatcg tgctcccgca
     3961 gggtcgtcac gaagatatcg taatcttccc gggtgccgat ctccgggttg atcctggtgg
     4021 gatccgtgat atcgtatccg tgaaggctcc cgggacgggc agcaaaatag ggggatgcat
     4081 agaggtcggt gatacccagt tccctgaggt aggggatcac cttcaccgcg tcggtgaacc
     4141 ggaaatcccg gttgaactgg acccggtagg tcgcaagagg tatccggagg tttcgaaaga
     4201 cgggcctttc catggctagc tcacctcatg ccccatcatc cctctctcct gtacgcgacg
     4261 gcgttgagcg gccccaggga aaggacggtc ccggtcttcc cgcagatgcg gggcggtgct
     4321 atttccccgc ttccgcccca acgggtcgaa agggtgtcgg agaccgcggc ccacgagcct
     4381 tcgtggagcg gcgatgcgac gtgcgcgttc tcttcactga agttgaaaaa gagggaaaca
     4441 tcattccctg gaagaggcat catgacaaag agggtgccct tgtccggcca gcatctgacg
     4501 agagggcggc cccctgtcat ctgccagggc ctgagcgagc gccgcaacga tgtcaggtgc
     4561 cggtaaaatg cgaagagtgg gcgacgggcc tcctcgaagc gggagacctt gtcgatcctc
     4621 gacagacgga atgttttctc tctttggggg tcgggtatct ctccgatgga cgggaaccag
     4681 gcgaattcgc gtgttctccc tgtccttacg gcctcgatga gcctttcgtc accgtggtcc
     4741 acgaaataca ggaagggaga ggtctcgccg tactcttccc ccatgaaaag aagagggatg
     4801 tacggcgaca gcaggacggc tgtcgccgcg accttaagcc tgtcgtgacc cacgagggag
     4861 gcgaggcggt cgccgccagg cctgtttccc gcctggtcgt gattctgaga gaagacgatg
     4921 aactgcgtgg ccggcctgtg tttcgacggg cttccgtgtt ttcttacgcg gtaggaggag
     4981 aactcgcccg tgtatacgta cccttcagcg aaagacttcg ccagattctc gatatcgccg
     5041 aaatcctggt aatagccctg tctctccccg gtgagaaggg tgtggagggc gtgatggaaa
     5101 tcgtcgttcc actgggcgtc gaggccgatg ccgccgttct cccggggtgt tatgatccgg
     5161 atgtcgttga gatcgctctc ggcaatgagg taggcggggg gattgccgga cagcgcggac
     5221 cccaccgtct ccgagagctc ctgcaggaag gtcctggcgc tgaagtcgaa tatcccatgt
     5281 atggcgtcga gcctgagggc gtccacgtgg taccgtacga accagtcgag ggcgtttcga
     5341 atgaaatagt tgcgcacctc gtcgctgaag gggccgtcga agttgagggc ctcgccccat
     5401 ggcgtccgat ggcggtccgt gaaatagggc ccgaagtttg ccaggtaatt gccttcgggg
     5461 ccgagatggt tgtagaccac atcgagaacg acagcgaagc cctgttcgtg acaggcgttc
     5521 accagggtct tgagcccccg gggccctccg tacgtattgt ggggggcgaa gggaaaggcg
     5581 ccgtcatatc cccagttccg gctgcccggg aactggctga cgggcatgag ctcgatggca
     5641 ttgataccca cgtccttgaa ataatcaagg cagccaacaa tggcctcgaa ggttccctgc
     5701 tccgtgaagg tgcccacgtg gagctcgtag atgatgtact cctcaagggc gcggccccgc
     5761 catccgtcgt cgctccatcg gaacgacgcg tggtccacga tccgcgaggg gccgtggacc
     5821 ccctccggct gcgattggga tgcgggatcg ggaaaagtgg agcttccgtc gatgacatac
     5881 cggtaaaggt cgccatcgcc gacgccgtcc acctttcctt cgaagtagtt ccccccggaa
     5941 ggcgtgaggg gctcccgcct ccctccggga cccaggagca tgtccacgga caccgtccgc
     6001 gcaaaaggcg cccacacccg gaaggatgtc gatccgtccg cggagggcac cgctccgaga
     6061 tgcgccggcg cgctcgtctt gtcgaatact ctcatctctc accagtatag cagaagatcg
     6121 tgaggcgcga gccgcggccc gtccctacag ttccacgagg ctcttcttgt tcgtgaggag
     6181 cgtgtaccac accgccaggt aatcccgcac ctgtctcgtg ataaggaagt tctggcggac
     6241 gaactcgcgg gcctgttcac ccatgctggc ggccatctcc gtgttgttga ggagctggcg
     6301 tatcctgaag gcgcacccct cgatggaatg aacgagaaag cccgtcacgc cgtgaactat
     6361 ctgcaggggt atgcctcctg ccgcgcctcc gatgacgggc ttgcctttcc acatgccttc
     6421 agcgacggtg aggccgaaac cctctttgag cgatttctgg aggatgacgc tggcgatggt
     6481 ctgcagtgcg ttgatgtccc tgtcgctgaa gggtgggaga aggaggatgt ggatgtcagg
     6541 atcgtccgaa gcgaacc
//
LOCUS       DAOQTR010000040         6518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13023_length_6518_cov_3.625870, whole genome shotgun sequence.
ACCESSION   DAOQTR010000040 DAOQTR010000000
VERSION     DAOQTR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13023_length_6518_cov_3.625870"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKN85_01745"
     CDS             <1..878
                     /locus_tag="PKN85_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43152.1"
                     /translation="QSADAMAEARRCIAGQVEGCIGCGECLRRCEAKAIDYSMRDELV
                     EMYFDGIVVAPGFDLYDPTEKTEFGYGRLKGVITGMEFERLTSVTGPTGGDIVMNGKT
                     PKRFYFIQCVGSRDRQSGARFCSRVCCMYTAKHACIVKDRIRDARIYISYIDVRTYGK
                     GYEEFYKSTQENGAMYIRGIPGEVTQGENGLLVRVEDMLSGELHEIEVDVVVLATGVR
                     PRAGTGEMCEMMSLERDEYGFISTSVVEPSRTSVDGIFVCGMASGPRDVPDTVASGGE
                     AASRCMEYVKESASS"
     gene            875..1780
                     /locus_tag="PKN85_01750"
     CDS             875..1780
                     /locus_tag="PKN85_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43153.1"
                     /translation="MKTGIFICHCGHNIKHTVDVKRLSEFFGKLPNVVVSQDYPFVCS
                     EPGQDLISESIEKYGLDRVIVASCTPSLHGEMFKDVLRKKDLNPFLLRRVSIREHCSW
                     VGDDIEENTEKAKRLILSGLYGVAHQVPLEEKMVEVNKTALVIGGGVSGLSAALFLSK
                     MGMQVYLVEKEPQLGGVVREIRNVWPARTSGAKITGDMEKELRSRQNVEILTSSELTG
                     FEGFFGNYEATLDTPAGERKIVIGGAVAAIGFAPFDPAIKPELQYGKDPRIVTTLDLE
                     RAGELALPENPRVAILHCIGSRDEQ"
     gene            1814..2533
                     /locus_tag="PKN85_01755"
     CDS             1814..2533
                     /locus_tag="PKN85_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT43154.1"
                     /translation="MGEAIRERYADSYVESFYMDMRAHPRGAEEFFEDTQEKGVLFTR
                     GNIGEIVPAPTGVLLKGEDTLLGETFEREFDLVVLSIGMSYPVDSQKLATLLKISLDK
                     DRFFLEAHIKLRPFDTAVKGIFIAGSCSGPKDMEESINHGRASALKLYGLLNLGYAFV
                     DPFISFVDPKRCSGCRMCEQACFSKAIKYDEEKKVVHVEEAACQGCGLCNATCPSSAI
                     SLRGYYDAILDDEISAFLEAI"
     gene            2567..2974
                     /locus_tag="PKN85_01760"
     CDS             2567..2974
                     /locus_tag="PKN85_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT43155.1"
                     /translation="MEDRTAKNPEIVGFACTYCTFKASEMAGSLRMKYPEGVKVIQVP
                     CSSRVDPAFVIKAIFEGADGVFVAGCHPGDCHFIKGNYYTRRKIAALKELFDAFGMKD
                     RLRLFWVSASEARRFVEKVTEFYNDIKEKKNAG"
     gene            2964..3968
                     /locus_tag="PKN85_01765"
     CDS             2964..3968
                     /locus_tag="PKN85_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT43156.1"
                     /translation="MQGKKIKNGALESALNTFLAEKEYLVMGFEKDASNVYHRVFKDS
                     LDNYALMSPVFATNGALYLRRLFAKGAQIILMLRPCEIRSYVELVKLTQIEPENIIAV
                     SVDCFGTVSSKTEGDIPAGEALKGLTKDAEKARWACANCRERRGVVGDAGIRIDANGD
                     YWALPYTDKGQAFLSLLEGETSEAPAQMAIGPAEPRPAFQIDMKEFTKDFSKCIMCMN
                     CRDMCPVCYCVDCVFNGDEYLPKGDALFNKVFRAGSTGMPRGKELFHLIRMYHVSQTC
                     VGCGACEEACPQGIPLTKYFKGVSERLQGMFSYMSGRSKDEIIPYLTFIEEELKDAED
                     "
     gene            3955..4413
                     /locus_tag="PKN85_01770"
     CDS             3955..4413
                     /locus_tag="PKN85_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNT43157.1"
                     /translation="MPKTEQALELTDYGRQDFAVCLGCKICASVCTVNDLSPGTNPQE
                     MLQRLFLGQDVAADEPLVRFCTGCYRCTGACPWEIRIPEVIRALRHELGTESPFEKAF
                     KGSVSLFGRVYEPYVLMKAVPFLLKGGYMKHVTRWMEYVGFHLPHKVKGM"
     gene            4415..5095
                     /locus_tag="PKN85_01775"
     CDS             4415..5095
                     /locus_tag="PKN85_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNT43158.1"
                     /translation="MEYGYYPGCSLTGSAHRLDKGIKKVFAKQGHTLREIPDWNCCGA
                     FEYGDRKELTGFSKRNLEKARGHFSEIVAPCPACYKNLKEADEEREFAVTHPLDLFDE
                     AFFAAMKQARDLTGQVFTPYYGCILLRPKETAIQNTNIMEETIARFGGEVSGEAVKDR
                     CCGGNQIFINKDVTEKLSRLVLDRSQGTIVVFCPLCHMAMKTFSGERKVVYYTDLLLH
                     IMGEGGAL"
     gene            5092..>6518
                     /locus_tag="PKN85_01780"
     CDS             5092..>6518
                     /locus_tag="PKN85_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43159.1"
                     /translation="MNVLILGGGISGVSAAKVVLKEGHRAIILESTPELGGTMARIAN
                     CRIGFKTFFDEIKDDPNLQVIRGASVTAAQRKADAFTVTLSDGNEVEAHRVIVAAGLS
                     PYDPVEYKGKRVLTSLEYDARIDQKSGELPEDFNKIGFFLCVGSRSKEYPLCSSVCCS
                     YTIREVKWTLQRAKPEIAVFYNDLRLFGQEFYLEKVYRDAGVRFVRANSRYFEEDEEG
                     VTVRYFVDGVLREERFNYIVLAIGLRPNPGLKDLSALFGFSLNEYGFVAEEDPLVTDA
                     QGVFACGGALEPMNIKDSIMTGFGAGVLAVKDADLLAAAARDEGLLRDEDPPEITMPD
                     GDFGSCVFYLGTADAGDAMFYEYMSARFIEAARTLKEAGKTVYIVTKNTVMPSYDEVT
                     FEKARREGVIIINLEEGEVFSASEGSFRLSGPGRDLTLTADRAISFGDYADRFKGREF
                     LSVYRSEPQLRWNPTRWSRKKYHIG"
BASE COUNT         1360 a         1903 c         2097 g         1158 t
ORIGIN      
        1 accagagcgc ggacgccatg gccgaggcac gacggtgcat cgccggccag gtcgagggat
       61 gcatagggtg cggggaatgc ctccgtcgct gcgaggcaaa ggcgatagac tactccatgc
      121 gggacgagct ggtggagatg tacttcgacg ggatcgtcgt cgccccgggt ttcgacctct
      181 atgacccgac ggagaagacg gagttcggct acggcaggct gaaaggtgtc ataacgggga
      241 tggagttcga gaggctcacc tcggtgacgg ggccgacggg tggagacatc gtcatgaacg
      301 ggaagacgcc gaagcgcttc tacttcatcc agtgcgtcgg cagccgcgac cgccagagcg
      361 gtgcccgatt ctgttcccgg gtctgctgca tgtacacggc aaagcacgcg tgcatcgtga
      421 aggacaggat aagggacgca cggatataca tctcttacat cgatgtgagg acctacggca
      481 agggctacga agagttctac aagagcaccc aggagaacgg cgccatgtac atccgcggca
      541 tccccggaga ggtgacgcag ggcgagaacg gcctcctggt gcgcgtcgag gacatgctct
      601 ccggcgagct ccacgagata gaggtggatg tggtcgtcct cgcgacgggc gtgaggcccc
      661 gggcgggtac cggggaaatg tgtgagatga tgtccctcga gagggacgag tacggtttca
      721 ttagcaccag cgtggtggag ccttcgagaa caagcgtgga cggcatcttc gtctgcggca
      781 tggcatcggg gccgagagac gtccccgaca cggtggcctc gggaggggaa gcggcgtcgc
      841 ggtgcatgga atacgtgaag gagagtgcct cttcatgaaa acagggatct tcatctgcca
      901 ctgcgggcac aacatcaagc acacggtgga cgtcaagagg ttgagcgagt tcttcgggaa
      961 actgccgaat gtggtcgttt cccaggatta ccctttcgtg tgctccgagc cggggcagga
     1021 cttgatcagc gagagcatcg agaagtacgg cctcgaccgc gtcatcgtag cgtcctgcac
     1081 cccgtccctc cacggcgaga tgttcaagga tgtgctgagg aagaaagacc tcaacccctt
     1141 cctgctgcgc agggtgagca tcagggagca ctgctcgtgg gtgggcgacg acatagagga
     1201 gaacacggaa aaggcgaaga ggcttatcct gtcgggtctc tacggcgtgg cgcaccaggt
     1261 tcccctcgag gaaaagatgg tggaggtcaa caagacggcc ctcgtcatcg ggggcggtgt
     1321 ttcgggcctc tcagcggcac ttttcctgtc gaagatgggc atgcaggtct atctcgtgga
     1381 aaaggagccg cagctcggcg gggtcgtgcg ggagataagg aacgtctggc cggcgaggac
     1441 gagcggcgcg aagataacgg gagacatgga gaaggagctc aggagcaggc agaacgtgga
     1501 gatcctgacg tcctcggagc ttacgggctt cgaaggcttc ttcggcaact acgaggcgac
     1561 cctcgacacc ccggcggggg agaggaagat cgtcatcggc ggggcggtcg cggccatagg
     1621 cttcgcgccc ttcgatcccg cgataaagcc cgagctgcag tacgggaagg acccgcggat
     1681 cgtgacgaca ctcgacctcg agcgcgcggg cgagcttgcc ctgcccgaga atccccgggt
     1741 cgcgatcctc cactgcatcg gttcccggga cgagcaatag ggcctgccga tctgctgcat
     1801 caacgccctg cgcgtgggag aggccatcag ggaaaggtac gccgactcct acgtggagtc
     1861 cttctacatg gacatgaggg cccatccgcg gggggccgag gagttcttcg aagacaccca
     1921 ggagaaggga gttctcttca cccgggggaa tatcggggag atcgtacccg caccgacggg
     1981 ggtcctcctc aagggtgagg acacgcttct cggcgagacc ttcgaacgcg agttcgacct
     2041 cgtggtcctc tcgatcggga tgtcctatcc cgtggacagc cagaagctcg ccacgcttct
     2101 caagatcagc ctcgacaagg acaggttctt tcttgaggcc cacatcaaat tgaggccttt
     2161 cgataccgcc gtcaagggta tcttcatcgc cggctcctgt tccgggccca aggacatgga
     2221 ggagtctatc aaccacggga gggcctcggc gctgaagctc tacggccttc tgaaccttgg
     2281 ctacgccttc gtggacccct tcatctcctt cgtggacccc aagcggtgca gcggctgccg
     2341 catgtgcgaa caggcctgct tctcgaaggc gataaagtac gacgaggaaa aaaaggtcgt
     2401 tcacgtcgag gaggccgcct gtcagggctg cgggctttgc aacgccacct gcccgtcatc
     2461 ggcgatcagc ctgaggggct attacgacgc catcctcgat gatgagataa gcgcatttct
     2521 ggaggcaata tgacctcgaa cgacgtcaag ggtagagggg aagtacatgg aagatagaac
     2581 ggcaaagaac ccggagatcg tgggttttgc gtgtacctac tgcacattca aggcctcgga
     2641 gatggcggga agcctgagga tgaagtaccc cgaaggggtg aaggtgatac aggtcccctg
     2701 ttccagcagg gtcgacccgg cctttgtcat aaaggccatc ttcgaggggg ccgacggcgt
     2761 tttcgtcgcc ggctgccatc ccggcgactg tcacttcatc aagggcaact attatacccg
     2821 ccggaagata gcggccttga aggagctttt cgacgccttc ggcatgaagg acaggctccg
     2881 gctcttctgg gtgagcgcct cggaggcgcg gcgtttcgtt gaaaaggtga cggaattcta
     2941 caacgacata aaggaaaaga aaaatgcagg gtaagaagat aaagaacggc gcgctcgaaa
     3001 gcgctctcaa cacgttcctc gcggagaagg aatacctcgt gatggggttc gagaaggacg
     3061 cgagcaacgt ctaccaccgg gtcttcaagg acagtctgga caattacgcc ctcatgagcc
     3121 cggtcttcgc gacgaacggc gccctctacc tgagaaggct cttcgcaaag ggcgcgcaga
     3181 tcatcctcat gctccggccc tgcgagatac ggtcctacgt ggagcttgtc aagctgacgc
     3241 agatagaacc cgagaacatc attgccgtct ccgtcgattg cttcgggacg gtctcgtcga
     3301 agacagaggg cgacatcccg gccggagagg cgcttaaagg gctcacgaag gatgccgaaa
     3361 aggcgcgctg ggcctgcgcg aactgccgcg agcggcgcgg tgtcgtcggt gacgcgggga
     3421 tacgcatcga cgcgaacggg gactactggg cccttcccta cacggacaag ggacaggcgt
     3481 tcctttcgct tctggaaggc gagacctccg aggcgcccgc gcagatggca atagggcctg
     3541 ccgaaccccg gccggccttc cagatcgaca tgaaggagtt cacgaaggac ttctcgaagt
     3601 gtatcatgtg catgaactgc cgcgacatgt gtcccgtgtg ctactgcgtt gactgcgtct
     3661 tcaacggcga cgaatacctg ccgaagggtg acgccctttt caacaaggtc ttccgagccg
     3721 ggtcgacggg gatgccccgc ggcaaggagc tcttccacct catccgcatg taccatgtct
     3781 cccagacctg tgtgggatgt ggtgcctgcg aggaggcctg ccctcagggg ataccgctga
     3841 cgaagtactt caagggcgtc tccgagaggc tccaggggat gttctcctac atgtcgggac
     3901 gcagcaagga cgagatcata ccgtacctta cctttataga ggaagaactg aaagatgccg
     3961 aagactgagc aggctctgga actcaccgat tacggaaggc aggacttcgc cgtgtgcctg
     4021 ggatgcaaga tctgcgcctc ggtgtgcacc gtgaacgacc tgtctcctgg cacgaacccc
     4081 caggagatgc tccagaggct cttcctggga caggacgtgg ccgccgacga gccgctggtg
     4141 cgcttctgca cgggatgcta ccgctgcacc ggcgcctgcc cctgggagat acgcataccc
     4201 gaggttatca gggccctgcg ccatgagctc ggcacggaat ctcccttcga aaaggccttc
     4261 aaggggtcgg tctctctctt cggcagggtc tacgagccct acgtgctcat gaaggccgtg
     4321 ccctttctct tgaagggggg gtacatgaag cacgtgacgc ggtggatgga atacgtgggt
     4381 ttccacctgc cccacaaggt aaaggggatg taacatggaa tacggctact atcccggctg
     4441 ctcgctgaca gggtcggcgc acaggctcga caagggcatc aagaaggtct tcgcgaaaca
     4501 gggccacacc ttgagggaga tccccgactg gaactgctgt ggggccttcg agtacggcga
     4561 caggaaggag ctcacgggat tctcgaagag gaacctcgaa aaggcgcggg ggcatttcag
     4621 cgagatcgtc gccccctgcc cggcgtgcta caagaacctc aaggaggcgg acgaggagcg
     4681 ggaattcgcg gtgacgcacc ctctcgacct cttcgacgag gccttcttcg ccgcgatgaa
     4741 acaggcccgc gacctcaccg gccaggtctt cacgccctac tacggctgca tcctccttcg
     4801 gccgaaggaa acggcgatac agaacacgaa catcatggag gagaccatcg cccgcttcgg
     4861 cggggaggtg tccggtgagg ccgtcaagga ccgctgctgc ggcggcaacc agatcttcat
     4921 caacaaggac gtcacggaga aactctcgcg cctcgtcctc gacaggtcgc aggggacgat
     4981 cgtcgttttc tgtcccctct gccacatggc gatgaagacc ttttccggcg aaaggaaggt
     5041 cgtctactac acggacctcc tgctccacat catgggagaa gggggtgccc tgtgaacgtg
     5101 ctgatcctgg gcggaggcat aagcggcgta tcggcggcaa aggttgtcct caaggagggc
     5161 cacagggcga tcatccttga gagcacgccc gagctgggcg gcaccatggc acgcatcgcg
     5221 aactgccgca tcgggttcaa gacattcttc gacgagataa aggacgatcc caaccttcag
     5281 gtcatacggg gcgcgtccgt cacggcggca cagaggaagg ccgacgcctt tacggtcacg
     5341 ttgagcgacg gcaacgaggt ggaggcccac cgggtgatcg tggccgccgg cctttcgccc
     5401 tatgaccccg tcgagtacaa gggtaagagg gtcctgacca gcctcgagta cgacgcccgc
     5461 atcgaccaga agagcggcga gctgccggag gacttcaaca agatagggtt tttcctctgt
     5521 gtcggctcgc gctcgaagga atacccgctg tgttcctcgg tgtgctgctc ctacaccatt
     5581 cgcgaggtca agtggaccct gcagcgggcg aaaccggaga tagccgtttt ctacaatgac
     5641 ctcaggctct tcggccagga attctacctc gagaaggtct accgcgacgc cggagtgcgc
     5701 ttcgtccgcg ccaattcccg ctatttcgaa gaagacgagg agggtgtgac ggtccgttac
     5761 ttcgtcgatg gagtgctcag ggaggaacga ttcaactata tcgtcctcgc catcgggctg
     5821 cgccccaacc ccgggctcaa ggacctctcc gccctcttcg gattttccct caacgaatac
     5881 gggtttgtcg ccgaggagga cccgctcgtg acggacgctc agggtgtctt cgcctgcggc
     5941 ggcgccctgg agcccatgaa catcaaggat tccatcatga cggggttcgg cgccggtgta
     6001 ctcgccgtga aggacgcgga cctcctcgcg gccgccgcca gggacgaggg cctcctgcgc
     6061 gacgaggacc cgccggagat caccatgccc gacggtgact tcgggtcatg cgtcttctat
     6121 ctcggcaccg ccgacgccgg cgacgcgatg ttctacgagt acatgtcggc ccgtttcatc
     6181 gaggccgccc gcacgcttaa agaggcgggc aagaccgtct atatcgtgac gaagaacacc
     6241 gtgatgccct cctatgacga ggtgaccttc gagaaggcgc gccgcgaggg cgtcatcatc
     6301 atcaacctcg aagaggggga ggtcttcagc gcgtctgaag gaagcttccg gttgagcggc
     6361 ccgggacggg acctgaccct caccgccgac cgcgccatct ccttcggcga ctacgccgac
     6421 cgtttcaagg gcagggaatt cctctccgtc taccgctccg agcctcagct gcgctggaac
     6481 ccgacgcggt ggagccgcaa gaaataccac atcggctt
//
LOCUS       DAOQTR010000041         6499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13077_length_6499_cov_3.560987, whole genome shotgun sequence.
ACCESSION   DAOQTR010000041 DAOQTR010000000
VERSION     DAOQTR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13077_length_6499_cov_3.560987"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..164
                     /gene="ffs"
                     /locus_tag="PKN85_01785"
     ncRNA           67..164
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKN85_01785"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            205..1794
                     /gene="dnaX"
                     /locus_tag="PKN85_01790"
     CDS             205..1794
                     /gene="dnaX"
                     /locus_tag="PKN85_01790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNT43160.1"
                     /translation="MSYTVIARRWRPQRFDDVVGQPHIITTLKNSITAGRIAHAFLFT
                     GPRGVGKTSLARILAKAVNCVNGPTAEPCNVCENCVGIAGGNFVDVIEIDAASTRGID
                     DIKELRETVRYLPMKAAYKVYILDEAHMLTDQARNAFLKTLEEPPGHNIFILATTELQ
                     KIPYTIMSRCQRFDFRRITEKEIVEQLKAICKKDGITFDEGAFHHVAIEADGSMRDAE
                     SILEQIIAYSGQHISEGDVINIIGVVEKEVVHRIVRSLLTKDLNDGLSAIETTLGDGY
                     DVNQVYRGLVSYLRNMMIVKAVGGLPDFISMGEDEYKALSGLIANIEYYEIQNMLYYM
                     LRSEDLMRGLFPRIALETLYINLYNLSQLRDVEKVIGELQRGEPQASRAGERTQGTGY
                     RERDTGSRERDMGDGRQEAEGDVSRAPSAPSREENASAAGGRPAGEGVRGFVEYLKKK
                     KPFLGSMFDSCECSVEGDDLIVLVDKKYGNFIKSEFEEVRKLASEFFGRAMNAEFRDA
                     GDRKKNILEEYVKEADSLFNL"
     gene            1806..2120
                     /locus_tag="PKN85_01795"
     CDS             1806..2120
                     /locus_tag="PKN85_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNT43161.1"
                     /translation="MSKGFNQILQQAKKMQDRLMKLQEEMADKTVEAQSGGGMVSCVV
                     NGKQEVVSLKISPEVLEEKDNELLEDLIVAAVNEGLDRSREMVQEEMSKVTGGMQMPF
                     GM"
     gene            2124..2720
                     /gene="recR"
                     /locus_tag="PKN85_01800"
     CDS             2124..2720
                     /gene="recR"
                     /locus_tag="PKN85_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNT43162.1"
                     /translation="MFYPEPIERLIENLTRLPGIGRKTATRLAFFLLNAKEGYITELS
                     RSLIDIKEKIRLCSVCFNITDTDPCVICRDERRDRTSVCVVEESSHMMVIESSNPGHF
                     RYHILHGVINPIEGVGPDDIRINELRDRVAREGISEVIVATNLNIEGNTTAHYIGELL
                     KPYDVRITRIASGIPVGGDIVYIDPLTIKSSLENRKKL"
     gene            2879..4672
                     /locus_tag="PKN85_01805"
     CDS             2879..4672
                     /locus_tag="PKN85_01805"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNT43163.1"
                     /translation="MKSANTEHKDLLQWVDDMARMCKPDSVYWCDGSKEEYDSLMAHA
                     VEVGSAIPLEKRPNSFLFRSQPSDVARVEDRTYISYPEESQAGPTNNWIEIDELKGIM
                     RGLYDGCMKGRTMYIIPFSMGPIGSPIAKIGIEITDSPYVVCNMHIMTRVGTRVLEQL
                     GTDGEFIPCMHSVGAPLEKGEKDVSWPCAPMDKKYISHFPDENLIWSYGSGYGGNALL
                     GKKCLALRIASAMARREGWMAEHMLILRLTSPEGRQYHIAAAFPSASGKTNLAMIQPT
                     VDGWKAECIGDDIAWMKIRPDGRLHAINPEAGFFGVAPGTSYASNPAAMDTIKENCIF
                     TNCGLTDDGDIWWEGMTEEPPTHTIDWRGQDWTPGSKDPTAHPNARFTAPAQQCPIIS
                     PDWEKLEGVPIDIFIFGGRRAATVPLVHEAFSWEHGVFMGATASSETTAANIGSIGDV
                     RRDPFAMKPFCGYHMGDYFQHWLDMGKRLGDKAPRIFYTNWFRKTADGRWLWPGFGEN
                     SRVLKWMCERVEGKAGAQETAIGYMPSVEDLDLTGLDVAAEDMKELLRIDVDAWKEEV
                     RDMEKHFAQFGDRLPQVLAGELKALAKRLGS"
     gene            4707..5174
                     /locus_tag="PKN85_01810"
     CDS             4707..5174
                     /locus_tag="PKN85_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43164.1"
                     /translation="MMASVRTGVCLGLMSLMLLAATATAACLVVSAGDGGGPLVSLPV
                     EGGCIVHLDFVNSIYLAPVRETFLHETGKGLSVIRVESPSAGVFEYYRLESDGPSAEL
                     YRNVGSIHLKSHDYRDHTITVGTTVLHLKDFAAGGDPLTISVHDGVCGSGQGR"
     gene            5174..5902
                     /locus_tag="PKN85_01815"
     CDS             5174..5902
                     /locus_tag="PKN85_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43165.1"
                     /translation="MGSARRSRTGFTGVVTAAITFVLLVSCAPMVPDYEGFNASGAAE
                     ALGRREQDIDALVRDGLVRFVLRPVAADDGYLFGERRVLGEPVTAQSGERLEIRWEGF
                     FPRYRLYYVPAPGHPGVVIGECYFPDGCNNGFFLAPDADGNTVPDRFRYVQWVSSDYG
                     HDDGVPGYLDRYRYVYDVAKDRLYLWHDLLFYRCPPPLSIPPYTCRTKCDPPCKVSTV
                     GNRTYPVVFKTRLVEERSLLGPQE"
     gene            6305..>6499
                     /locus_tag="PKN85_01820"
     CDS             6305..>6499
                     /locus_tag="PKN85_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43166.1"
                     /translation="MGIEKKTREPVVSPRCFSPLTRVDATRIETGQRKEIGKLRCVPG
                     NDISYRDIVLFLLERTRYHGK"
BASE COUNT         1438 a         1851 c         1967 g         1243 t
ORIGIN      
        1 tgtgtacgtg ctaaaacgcg taccgagggt tcaaatccct ctctctccgc cattttgatt
       61 gagaatggcc aggccctgcg caacgtggag gttatgaacc ccgtcaggtc cggaaggaag
      121 cagcggtagt agctcgaccg tgtgccgcag gtagcctggc ttttttttgg tacacaccac
      181 gaacccccga agaggcgttc acatttgagc tacacggtca tagcaagacg ttggagaccc
      241 cagagattcg atgatgtggt cggtcagccg cacatcatta ccaccctcaa gaattccata
      301 acggcaggca ggatagcaca cgcctttctt ttcacggggc cccgtggcgt tggaaagacc
      361 tcgctggccc ggatcctcgc aaaggcggtc aactgcgtga acggcccgac ggctgagccc
      421 tgcaatgtct gcgagaactg cgttgggata gcgggcggca atttcgtgga cgtcatcgag
      481 atagacgccg cttcgacgcg ggggatcgat gacatcaagg aactgcggga gaccgtgcgc
      541 taccttccca tgaaggcagc ctacaaggtc tacatcctcg atgaggccca catgctgacg
      601 gaccaggcgc gcaacgcctt cctcaagacc ctggaggagc ccccgggaca caacatcttc
      661 atccttgcga ccacggaact gcagaagatt ccctacacga tcatgtcccg ctgccagcgc
      721 ttcgatttca ggcgcatcac ggaaaaggag atagtggagc agctcaaggc catctgcaaa
      781 aaggacggga taactttcga cgagggggcc ttccatcatg tcgcgatcga ggcggacggt
      841 tccatgcggg acgccgaatc catccttgag cagataatag catacagcgg gcagcacatc
      901 tccgaaggtg atgtcatcaa catcatcggg gttgtcgaga aggaggtcgt ccatcgcatc
      961 gtgaggtctc ttctcacgaa ggacctgaac gacggacttt cggcgatcga gacgaccctc
     1021 ggcgatggct acgacgtgaa ccaggtctat cgcggtctcg tctcctacct caggaacatg
     1081 atgatcgtga aggccgtggg gggactgccc gatttcatct ccatgggcga ggacgagtac
     1141 aaggccttga gtggtctcat cgcgaacata gaatattacg agatccagaa catgctctac
     1201 tacatgctcc gcagcgaaga cctcatgagg gggcttttcc cgcggatcgc gctggagact
     1261 ctctacatca acctctacaa cctgtcgcag ctcagggacg tggaaaaggt cataggagag
     1321 ctgcaacggg gagagccgca ggcttcgcgg gcaggggaga ggacacaggg aacgggatac
     1381 agggaacggg atacagggag cagggaacgg gatatgggag acggcaggca ggaagcggag
     1441 ggtgatgttt cccgtgctcc gtccgcgccg tcccgtgagg agaatgcttc agcggcgggc
     1501 gggcgcccgg cgggggaggg cgtccgtggc ttcgtggaat acctcaagaa gaagaagcct
     1561 tttctcggaa gcatgttcga ctcctgcgaa tgttcggttg agggggatga tctcatcgtc
     1621 ctcgtcgaca agaagtacgg caatttcata aaaagcgaat ttgaagaggt ccgcaagctc
     1681 gcgagcgagt tcttcggcag ggcgatgaac gcagagttca gggacgccgg cgacaggaag
     1741 aagaacatcc tcgaggagta cgtgaaggag gcggattctc tcttcaatct ttaggaggag
     1801 acagcatgtc caagggtttc aaccagatat tgcagcaggc caagaagatg caggaccgtc
     1861 tcatgaagct ccaggaagag atggccgaca agacggtgga ggcacagtcc ggtggcggta
     1921 tggtgagctg tgtcgtcaac ggcaagcagg aggttgtctc tctgaagata tccccggaag
     1981 ttctcgagga aaaggacaac gaactcctgg aagacctcat tgtcgcggcc gtcaacgaag
     2041 ggctcgaccg ctcccgcgag atggtccagg aggagatgtc gaaggtgacg ggcggcatgc
     2101 agatgccctt cgggatgtag gacgtgttct accccgaacc gatcgaacgg cttatcgaga
     2161 acctgacgcg gcttcccggc atcggccgga agacggcgac acggctcgcc ttcttcctgc
     2221 tcaacgccaa ggaggggtat atcacggagc tgtcgagaag cctcatcgac atcaaggaga
     2281 agatacgcct ctgcagcgtt tgcttcaata tcaccgatac cgacccctgc gtgatatgca
     2341 gggacgagag gcgtgacagg acctcggtat gcgttgtgga agagtcttcc cacatgatgg
     2401 tcatcgagtc ctccaacccc gggcatttcc ggtaccacat cctccatggc gtcatcaacc
     2461 ccatcgaggg cgtgggcccc gacgacatca ggataaacga gcttcgcgac cgcgtcgccc
     2521 gcgaaggcat aagcgaggtc atcgtcgcca cgaacctcaa catcgaaggc aacaccaccg
     2581 cccactacat cggcgagctc ctcaaaccct acgacgtaag gatcacaaga atagcctcag
     2641 gcatccccgt aggcggagat atcgtctaca tcgaccccct gaccatcaaa agctccctgg
     2701 aaaacagaaa aaagctatga agacaggtga caggtgatag gtcataggtg ataggaaggg
     2761 atccttcctt cccatgaccc ataacccatg accccttatc tgcttcttct tctcttgcaa
     2821 tcccaccacg atttgttgtt aactatttcg ctgcagatat tgacaggagg aactacagat
     2881 gaaaagcgcg aacacagaac acaaagatct tcttcaatgg gtcgacgaca tggcgcgcat
     2941 gtgcaaacct gacagcgttt actggtgtga cggttcaaag gaggagtacg acagtctcat
     3001 ggcccatgcc gtggaggtag ggtcggccat accactcgag aagcggccca acagctttct
     3061 cttccgttcc cagccgtccg atgttgcccg tgttgaggat cgcacctaca tcagctaccc
     3121 tgaggaatcc caggccggtc ccacgaacaa ctggatcgag atcgacgagt tgaagggcat
     3181 catgcgcggt ctctatgacg gttgcatgaa gggccgtacc atgtacatca tccccttctc
     3241 gatgggcccc atcggctcgc ccatagcgaa gatcggcatc gagataacgg acagccccta
     3301 cgttgtctgc aacatgcata tcatgacacg tgtggggacg cgggtgctcg agcagttggg
     3361 cacggacggc gaattcatcc cctgcatgca ttccgtcgga gcgcccctcg aaaagggaga
     3421 gaaggacgtt tcctggccct gtgcgcccat ggacaagaaa tatatcagcc atttccccga
     3481 tgagaacctc atatggtcct acgggtccgg ttacggcggc aacgcgctgc tcggcaagaa
     3541 atgtctcgcc cttcgcatcg cctccgccat ggcacggcga gagggctgga tggcggagca
     3601 catgctcatc ctgcgtctca caagtcccga gggaaggcag taccacatag cagccgcgtt
     3661 cccttcagcc tcgggcaaga ccaaccttgc catgatacag cccacggtag acggctggaa
     3721 ggccgagtgt atcggtgacg acatagcctg gatgaagatc aggcccgatg ggaggctcca
     3781 tgctatcaac cccgaggcgg gtttctttgg agtggcgccc ggcacgtcct atgcgtccaa
     3841 cccggccgcc atggacacga tcaaggagaa ctgcatcttc acgaactgcg gcctcacaga
     3901 cgatggcgat atctggtggg aggggatgac ggaagagccg ccaacccaca ccatcgactg
     3961 gcgcggacag gactggacac ccggatcgaa ggacccgacg gcgcatccca acgcccgctt
     4021 cacggccccg gcgcaacaat gccccatcat cagtcccgac tgggagaagc ttgaaggcgt
     4081 ccccattgac atcttcatct tcggaggccg ccgtgccgcc acggtaccgc tcgtccatga
     4141 ggcattcagc tgggaacacg gtgttttcat gggggcgacc gcctcgtcgg agacaacggc
     4201 cgcgaacatc ggctccatcg gcgacgtgcg ccgcgacccc tttgcgatga agcccttctg
     4261 cggctaccac atgggagatt acttccagca ctggctcgac atgggcaagc gcctgggaga
     4321 caaggcaccc cgcatctttt acaccaactg gttcagaaag accgccgacg gcaggtggct
     4381 ctggcccggt ttcggggaga acagccgcgt cctgaaatgg atgtgcgaga gagtcgaagg
     4441 caaggcggga gcacaggaaa cggccatagg ctacatgccg tcggtggaag acctcgacct
     4501 caccggactt gatgtggccg ccgaggatat gaaagagctg ctgcgcatag atgtcgatgc
     4561 ctggaaagag gaggtcaggg acatggagaa gcatttcgcc cagtttggag accgcctgcc
     4621 ccaggttctg gcaggggaat tgaaggcgct cgcgaagagg ctgggaagct agacggccgg
     4681 gaccatcgcg tcctttcagg aggttgatga tggcgtccgt ccgcaccggc gtgtgcctgg
     4741 gtctgatgtc cctgatgctg ctagcggcaa ccgcgacggc ggcatgcctc gtggtatcgg
     4801 cgggagacgg gggaggaccc cttgtttcac ttcccgtcga gggtggatgc atcgttcacc
     4861 ttgatttcgt caattcgatc taccttgctc ccgtgcggga gaccttcctt catgagaccg
     4921 gcaaggggct ttcggtcata cgggtggaaa gcccctccgc cggtgtcttc gagtactaca
     4981 ggcttgagag cgacgggccc tcggcggaac tgtaccgcaa cgtgggctcc attcacctga
     5041 aaagccacga ttacagggac cacaccatca ccgtgggtac aaccgttctt catctgaagg
     5101 actttgccgc gggaggcgac ccgttgacca tctccgtgca tgatggggtc tgcgggtcgg
     5161 gtcaggggcg ataatgggtt ccgcgcggag gtcccgcacc gggtttacgg gggttgtcac
     5221 cgccgccata actttcgtgc ttctcgtatc atgcgcgccc atggtcccgg actatgaggg
     5281 ctttaacgct tccggcgccg cagaggccct tggccgtcgc gagcaggata tcgacgccct
     5341 ggtgagggac ggactcgtaa ggttcgtctt aaggcccgtg gcggcagacg acgggtacct
     5401 cttcggtgag aggagggtcc tgggagagcc cgtgacagcc cagtcgggag agaggctgga
     5461 gatacgatgg gagggtttct tcccgagata ccgcctctat tatgtccccg cacccgggca
     5521 ccccggcgtt gtcatcggtg aatgctattt tcccgacggc tgcaacaacg ggttcttcct
     5581 tgcccccgac gcggacggga acactgtccc cgacaggttt cgctacgtcc agtgggtaag
     5641 cagcgattac gggcatgacg atggcgttcc gggctacctc gaccgttacc gttacgtcta
     5701 cgacgtggca aaggaccggc tctatctctg gcacgacctt ctcttctacc ggtgcccccc
     5761 gcctctgagc atcccaccct acacgtgcag gacgaagtgc gaccctccct gcaaggtgag
     5821 caccgtcggc aacaggacct accccgtggt attcaagacg cgcctcgtcg aagagaggtc
     5881 cctcctcgga ccacaggagt gatacgcaac cacttcaagg acttacgccg tgacaggtct
     5941 tgtctttcgc gttgccgcta atgtaatatg cacgtgtata gactgtggcg gatatctttt
     6001 tccggagggt gggtaaatga gaggaaggcc ttgttggttt gccctgtttc tggctgtcat
     6061 tgtgcccgtg agtctcttat gcaccggcaa tgcggtctca gcgacgggga aggagaaaga
     6121 ctcgatggga acctgcataa aggaggttcg tatcacccgt ggacggaacc ccttccggga
     6181 atgggacgca agtccggcag ggtcgaagca ggcgggagcg cccgttaggg ccgccgccct
     6241 tgcaggagat gattttgaca cgcctcctgg gctttctcgg tgtacctttg aaggcggcag
     6301 attgatgggg atcgagaaga agacccgcga gcctgtcgtg tctccgcggt gtttttcccc
     6361 gctgacgaga gtcgacgcca cgaggataga gacggggcag cgcaaggaga tcgggaagct
     6421 tcggtgcgtc cccggtaatg acatctctta ccgtgatatc gtgctctttc tcctagaaag
     6481 aacacgatat cacggtaag
//
LOCUS       DAOQTR010000042         6492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13097_length_6492_cov_3.637564, whole genome shotgun sequence.
ACCESSION   DAOQTR010000042 DAOQTR010000000
VERSION     DAOQTR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13097_length_6492_cov_3.637564"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1387
                     /locus_tag="PKN85_01825"
     CDS             113..1387
                     /locus_tag="PKN85_01825"
                     /inference="COORDINATES: protein motif:HMM:NF041136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140132 - iron-sulfur cluster carrier
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein MrpORP"
                     /protein_id="HNT43167.1"
                     /translation="MAECGQTTQDKDMDDLFLQERLSRINRTLMVLSGKGGVGKSTVA
                     VNVALSLAAQGQRVGLLDVDIHGPSIPKMLGLNGQKVGVKDNEIIPIEVYGKLHVVSM
                     GLLLEHDDQPVVWRGPLKYNVIKEFLQHVMWGNLDYLVIDAPPGTGDEPLSIGQLIKE
                     RASAIIVTTPQQVATIDVAKCITFCKQLDLPVAGIVENMSGFICPHCGKEVDIFMKGG
                     GKELAERMNVPFLGAVPLDPDIVKSGENGIPYVLSYSTSETAKRFDEIVDKITAVYSS
                     GNIDIPRPPAKEENTEGSVIPPVSGAGQGDIAAQGGTMKFAVPTNDGKLCMHFGHCEA
                     FALIDTDAEGKVMNETYVTPPPHEPGLLPPWLAQQGVNCIIAGGMGSRAQQLFAQQGV
                     RVVTGAQEADPKKAVENYLKGTLVTGANTCDH"
     gene            1459..2211
                     /locus_tag="PKN85_01830"
     CDS             1459..2211
                     /locus_tag="PKN85_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNT43168.1"
                     /translation="MKVLEDIISSITVDAPVTDVTKGIFWTALVSRHCGLASTMVYDW
                     KMGGAKEPEETSYLQMSAASLARGALLDDTASASLGLAAINSLIDVDTVRCVDLNAGH
                     YLVENGQGKNITVIGHFPFVDELKKVAGNLWVIEKRMQPGDRPEEETATCLARSDIIA
                     ISSTTLINHTLGNILDLCPSGSTRMLLGPTTPLTSALFDHGIDIISGSVVTDPETALL
                     HIRQGANFRALKRTGSIRLVTLMKDQGGHRLQ"
     gene            2208..2621
                     /locus_tag="PKN85_01835"
     CDS             2208..2621
                     /locus_tag="PKN85_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNT43169.1"
                     /translation="MNEYTPIVMDHFNNPRNVGIINNHDGMGEIGDPNCGDFLRVFIK
                     VEDNIIIAIRYQIRGCPASIACASVMTELAVGKDLDEAMMIDDMDIVKALGGLPEYKL
                     HCSNLGATGLKKAILNHLARYMSSVKEQQQADKPK"
     gene            2642..3541
                     /locus_tag="PKN85_01840"
     CDS             2642..3541
                     /locus_tag="PKN85_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT43170.1"
                     /translation="MKSLLMALALVVLILSAGPAPGGAEGISPVVTTKWLAENMKDPR
                     LVVTDIRRVEEYKEEHIPGSVSLTYNAWRTMEKGISCQLPPKDDIEDNLRSAGIDRDT
                     VVVIVGKTDTDLDRAHPARVAWTMKYAGVANVAILDGGYSKWKAEGRPLAGGWTTAAP
                     SSLRCAWNKDLLCSKDCLKEKAGRVTIVDTRIHSHFTGKVQDPGVRKKGHIPGSVNLP
                     YTFAFKKDGTFQSRDRLAHRAVRAVGKDRKKEIVILCCTGRFSPTWWFMLSQVLGYEK
                     VSIFDGSMEEWCADASAPLVEDR"
     gene            3849..5162
                     /locus_tag="PKN85_01845"
     CDS             3849..5162
                     /locus_tag="PKN85_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT43171.1"
                     /translation="MKNDDMAEVDLAKGQRTALFASAVTFGLAAVKALIGYGFHSPLL
                     VADGFHTFSDFTVIFASWFGLFLAARKKTERFPYGLYKAETLVSFIIGLLVTWAAIEV
                     FLEGVRKFGPPASTGAFPVLPVTVSCVSMAIGYFLARQEGSVGNEINSQSLKTIAQDS
                     YLNIAVSFAVLAGILAAWFSIPYVEGVLLVIISLLVLKLGLETIWFSLLILLDANLDR
                     PLQSDIIREISDIQGVTGVRNVKIRQAGPFRLVECNIESSPNAPLYQAHGVADRVEEH
                     IKANFPYIESVYVHVEPGKSKTTVAIIPVRDVDGLNSRVYDHFGRAPYFIVVKIGGNG
                     VEIEDFYYNEHLGKKGKIHVGIKVIRELTRYGLNTLFTSQIGEISFHMLKDAFVDIYK
                     AREDESVAEVIERFRHGDIEVLKAPTHTAEKSLAEGVIRTEGNES"
     gene            complement(5338..6054)
                     /gene="prxU"
                     /locus_tag="PKN85_01850"
     CDS             complement(5338..6054)
                     /gene="prxU"
                     /locus_tag="PKN85_01850"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259907.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5803..5805),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HNT43172.1"
                     /translation="MAQQKESCVKTAKGPISAPSAEGSSASAPIEREVAMVTARVGKP
                     APDFEANAFVDGGFKNVTLSEFKGQWVVLCFYPGDYTFVUPTELAAVAVKHEELRTLG
                     VQVLACSVDSSFVHKIWNEDELSKMVKGGLPFPMLSDAGGRIGKVYGVYDEEGGVDIR
                     GRFIIDPDGIIQAMEVITPPVGRNVAEFIRQVQAFQHVRATGEATPSGWRPGKVTLTP
                     SPDLVGKVWTVWQPQMAFED"
     gene            complement(6145..>6492)
                     /locus_tag="PKN85_01855"
     CDS             complement(6145..>6492)
                     /locus_tag="PKN85_01855"
                     /inference="COORDINATES: protein motif:HMM:NF020475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HNT43173.1"
                     /translation="VSGIFERKGFDVVSVMCKVGRVPKEEIGLEDEHKMFRGEFEAMC
                     NPILQALLVNDAKCEFNILLGLCVGHDSLFLKYADAPSTVLAVKDRVMGHNPLAAVYL
                     SEGYYSWLENPVK"
BASE COUNT         1454 a         1952 c         1857 g         1229 t
ORIGIN      
        1 ggaggcacag cggcagggaa aggcgatcgt tgaatatgca ccggacctcc ccgtgagcaa
       61 ggccttgcgg gagatatcgg agcaaataat atcaatatac aaggagtaac gtatggcaga
      121 gtgtggacag acaacacagg ataaggacat ggacgacctt ttcctccagg agcgcttgag
      181 ccggataaac cggaccctta tggtcctttc cggcaagggt ggagtgggca agagcacggt
      241 agcggtgaat gtggccttga gcctggccgc gcagggacag agggtcggcc tcctagacgt
      301 cgacattcac gggcccagca ttccgaaaat gctcgggctg aacggccaga aagtgggcgt
      361 caaggacaac gagataatcc ccattgaagt ctacgggaaa ctccacgtcg tatcaatggg
      421 gctcctcctc gagcacgacg accagcccgt ggtgtggagg gggcctctca agtacaacgt
      481 catcaaggaa tttctccagc atgtcatgtg ggggaacctc gactatcttg tcatcgatgc
      541 ccctccgggg accggcgacg agccgctctc catcggacag ctcatcaagg aacgagccag
      601 cgccatcatc gtcaccacac ctcagcaggt ggcgaccatt gacgtggcaa agtgcatcac
      661 cttctgcaag caactggacc ttcccgttgc cggcatcgtg gagaacatga gcggattcat
      721 ctgcccccac tgcggcaagg aggtcgacat cttcatgaaa ggcggcggaa aggagctcgc
      781 ggagcgcatg aacgttccct tcctcggcgc cgtacccctg gatccggaca ttgtaaaaag
      841 tggtgagaac gggatcccct acgtcctttc ctacagcaca tcggaaacag cgaaacgttt
      901 cgacgagatc gtggacaaga tcaccgcggt ctactcatca ggcaatatcg atatcccccg
      961 gcccccggca aaagaagaga atacggaagg ctctgtcata ccacccgtga gtggcgcggg
     1021 ccagggggat atcgctgctc agggaggcac catgaaattc gccgtaccta cgaacgatgg
     1081 caagctctgc atgcatttcg gccattgtga ggcctttgcg ctcatcgaca ccgatgccga
     1141 gggcaaggtc atgaacgaaa catacgtgac gcctccgccc cacgaaccgg gcctcctccc
     1201 gccgtggctc gcgcagcagg gcgtgaactg catcatagcc ggaggcatgg gaagccgcgc
     1261 ccagcagctc ttcgcccagc agggcgtcag ggtcgtgaca ggcgcacagg aagcagaccc
     1321 gaagaaggcg gttgagaatt accttaaagg aacgctcgtc accggcgcga acacatgtga
     1381 ccactaagtg aagccgggat cacggggagc gggctggcgc gtcgcgccca cccgctcccc
     1441 ccgaaagagg tcgctggaat gaaagtactt gaggacatca tatcctcgat caccgttgac
     1501 gctcccgtca cggacgttac aaaggggatc ttctggacgg cgctcgtgag caggcattgc
     1561 ggcctggcat cgacaatggt ctatgattgg aagatgggag gcgccaagga gccggaagag
     1621 acctcctacc tgcagatgag cgccgcctcg ctcgccaggg gcgcgctcct tgacgacacc
     1681 gccagcgcgt cccttgggct tgccgcgatc aactccctga tcgacgtcga taccgtgcga
     1741 tgcgtcgatc tcaacgccgg acattatctc gtcgaaaacg gacaggggaa gaacattacc
     1801 gtgatcggtc attttccctt tgttgacgag ttgaaaaagg ttgcgggaaa tctctgggtt
     1861 atcgaaaagc gcatgcagcc cggagaccgc cccgaggagg aaaccgcgac ctgcctcgcg
     1921 cggtcggaca taatagccat ctccagcacg accctgatca atcacaccct cggcaatata
     1981 ctcgatcttt gcccttcggg cagtaccagg atgctgctcg gcccgacgac gccgctgacg
     2041 agcgcgctct tcgaccatgg catcgacatc atctccgggt ccgtagtcac cgatcctgag
     2101 acggcgctcc tgcacatacg ccagggagcg aatttcaggg cattgaaacg gacgggttcc
     2161 atccggctcg tcactctaat gaaagaccag ggaggacaca gactccaatg aacgaataca
     2221 ccccgatcgt catggaccat ttcaacaacc cccgcaacgt cggcatcatc aacaatcacg
     2281 atggaatggg agagatcggc gacccgaact gcggggactt cctcagggtc ttcataaagg
     2341 ttgaagataa catcatcatc gccatacggt atcagatcag gggatgcccc gcgtccattg
     2401 cctgcgcgag cgtcatgaca gagcttgccg tcggcaagga cctcgacgag gccatgatga
     2461 tcgatgatat ggacatagtc aaggctttgg gaggactgcc ggaatacaag ctccattgct
     2521 ccaacctggg tgcgacgggg ttgaaaaagg cgatcctgaa ccatcttgcg cgttacatgt
     2581 cgagcgtgaa agagcagcag caggctgaca aacccaaata gcgcaacgtg aggaaaaaga
     2641 catgaaatct ctactcatgg cactggccct ggtcgttttg atcctctccg cgggaccggc
     2701 cccaggcggc gccgagggga tatcccccgt cgtcacgacg aagtggctcg cggaaaatat
     2761 gaaagatccc cgccttgtcg tcaccgacat cagaagggtc gaggaataca aggaagaaca
     2821 catacccggg tccgtcagtc tgacatacaa cgcctggagg accatggaaa aagggatctc
     2881 atgccagctc cccccgaagg acgatatcga agacaacctg aggtccgcag ggatagaccg
     2941 tgacactgtt gtcgtcatcg tcggcaaaac ggacaccgac cttgaccggg cacatccggc
     3001 acgggtcgcc tggacgatga aatatgccgg tgtcgccaat gtggctattc ttgacggcgg
     3061 atacagcaag tggaaggccg aggggcgacc ccttgccggc ggttggacga cagccgcgcc
     3121 gagctctctt cgctgcgcct ggaacaagga cctgctctgt tcaaaagatt gcctcaagga
     3181 aaaggcaggt cgcgtcacca tagtcgatac gaggatccac agtcatttca ccggcaaggt
     3241 tcaagacccg ggcgtcagga aaaagggaca cattcccggc agcgtcaacc tgccctatac
     3301 cttcgcgttc aagaaggacg gcaccttcca gagcagggac aggctcgccc accgggctgt
     3361 tcgcgccgtc ggcaaggaca ggaagaagga gatcgtcatc ctttgctgca cgggccgttt
     3421 ctcccccacg tggtggttca tgctcagcca ggtcctggga tatgaaaagg tctccatttt
     3481 cgacgggtcc atggaggaat ggtgcgcgga cgcctcggcg cctctcgtcg aggaccggta
     3541 agcaggacac gggcagtttg ctgatgcgct gagagagcat atatcgcacc gtcagaagcg
     3601 gaggtcagca gtgtcattcg gattcagagg caggcacggc catcgcaggc acggcggaag
     3661 gaccgtcacc ggccaaaccg gagaccaggg ccgcgcggct accattccac cacggcagtg
     3721 catctgcccg cattgcggca tgataacgga caaggtcccg gggcaaccct gtttcctgac
     3781 gccctgcccc cgttgtcagg gcatgatggc gggacggttc ccggcggagg aacgacggga
     3841 acaaagtcat gaagaatgac gatatggccg aggtcgatct ggcaaagggt caacgaacgg
     3901 cccttttcgc ctctgccgtg accttcggcc tcgccgccgt caaggccctt atcggctacg
     3961 ggttccattc tccccttctc gtcgctgacg gcttccacac cttttccgat ttcacggtga
     4021 tattcgcctc gtggttcggg cttttcctgg cagcccgcaa gaagacggag cgtttccctt
     4081 atggcctcta caaggccgaa accctcgtca gtttcatcat cggactcctc gtgacctggg
     4141 cagcgatcga ggtcttcctc gagggggtca ggaagttcgg tccccccgca tcgacaggcg
     4201 ccttccccgt gttgccggtc accgtctctt gcgtctccat ggccatcggg tatttcctgg
     4261 cgcgccagga aggctccgtc gggaatgaga tcaattccca gtcgctcaaa acgatagccc
     4321 aggattccta tctcaatatt gccgtgtcct tcgcggtcct tgcgggcatc ctggccgcct
     4381 ggttttccat cccctatgtt gagggagtgc tcctcgtcat catctccctg ctcgtcctga
     4441 agctgggtct cgagaccatc tggttttccc tgctcatcct cctcgatgcg aacctcgacc
     4501 gtccgctgca gtcggatatc atacgggaga tcagtgacat acagggtgtg accggggttc
     4561 ggaacgtgaa gatacgacag gcggggccct tcagactggt cgagtgcaat atcgaaagca
     4621 gccccaacgc gcctctctac caggcacatg gcgtggccga cagggtggag gaacacatta
     4681 aggcgaactt cccttacatc gagtccgtgt acgtccacgt tgagcccgga aaaagcaaga
     4741 ccaccgttgc cattatcccc gtgagggacg tcgatggcct caattcaagg gtctatgacc
     4801 atttcggcag ggcaccctat ttcatcgtcg tgaagatcgg ggggaacgga gttgagatcg
     4861 aggatttcta ttacaacgag cacctcggca aaaaaggcaa aatccacgta gggatcaagg
     4921 tgatcagaga actgaccagg tacgggctga acaccctttt cacctctcag ataggggaga
     4981 tatcctttca catgctcaag gatgccttcg ttgacatata caaggcccgc gaggatgaat
     5041 cggttgcgga ggtcatcgaa cgctttcgcc atggggacat cgaggtcttg aaagctccca
     5101 ctcataccgc cgagaagtcg ctggccgaag gcgtgatacg aacggaaggg aacgagtcat
     5161 gacgggtccg gcgccgtcga gaagatggtg gaaccgggtt atcccgtcgt cacgggcatc
     5221 ggccggtacg gatcccccaa caggatgtgt ctggggaacg cagacgtggc ggtcttctga
     5281 aaatagtacc ggggccggga atcacgcacg gtttcccggc cccggtactt tgatcgccta
     5341 atcttcgaac gccatctgcg gctgccagac cgtccagacc ttcccgacga gatcgggcga
     5401 gggcgtgagc gtaaccttgc ccggccgcca cccggacggg gttgcctcgc cggtcgcgcg
     5461 cacgtgctgg aatgcctgca cctgcctgat gaactccgcc acgttgcggc ccacgggagg
     5521 cgtgatgacc tccatggcct ggatgatacc gtcgggatcg attatgaaac gtccgcggat
     5581 gtcgacccct ccctcctcat cgtacactcc gtagaccttt ccgatacgtc cccccgcgtc
     5641 tgaaagcatc ggaaagggca gaccaccctt gaccatcttc gacaactcgt cctcattcca
     5701 gatcttgtga acgaaactgc tgtcgacact gcacgcgagg acctgcaccc cgagtgtcct
     5761 cagctcttcg tgcttgacgg cgaccgccgc cagttccgtc ggtcagacga aggtgtaatc
     5821 cccgggatag aagcaaagca ccacccactg acctttgaac tcggaaaggg taacgttctt
     5881 gaacccaccg tcgacgaagg cgttcgcctc aaaatccggc gccggtttgc ccactctcgc
     5941 tgtcaccatg gccacctccc tttcaattgg tgcggatgct gaggaccctt cggccgatgg
     6001 ggcgcttatc gggcctttgg ccgtcttgac acatgattcc ttctgttgcg ccatgttcac
     6061 ctcccgtcaa tacaataagt ctagcccaca ataccgggct tgaaaagcga aacggtcccg
     6121 gcacagatcc cggcggtcct tcccttactt gaccgggttt tccagccagg aataataccc
     6181 ttccgacaga tacacggccg cgaggggatt gtgtcccatg acgcggtcct tcaccgcgag
     6241 caccgtggag ggcgcgtcgg catatttcag gaaaagcgag tcatggccga cacacagccc
     6301 caggaggatg ttgaactcgc atttcgcatc attcaccaac agggcctgca gtatggggtt
     6361 gcacatggcc tcgaattcgc cccggaacat cttgtgttcg tcctcgaggc cgatctcctc
     6421 cttcggcacc ctgccgacct tgcacatcac cgacacaacg tcgaagcctt ttcgctcaaa
     6481 tatcccggaa ac
//
LOCUS       DAOQTR010000043         6486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13119_length_6486_cov_3.605194, whole genome shotgun sequence.
ACCESSION   DAOQTR010000043 DAOQTR010000000
VERSION     DAOQTR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13119_length_6486_cov_3.605194"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKN85_01860"
     CDS             complement(<1..392)
                     /locus_tag="PKN85_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43174.1"
                     /translation="MHREDEPEKGPKDHVSEGLKTACNVSPEKTAKLLEDLRLHQVEL
                     EMQNEELKKAQQVIEEGRQRLADLYDFAPVGYLTLNAQNVVEEANLTAATLLGTVRQV
                     LVGASFLQYVDSHYAAEYRRHHERVVKS"
     gene            complement(404..3409)
                     /locus_tag="PKN85_01865"
     CDS             complement(404..3409)
                     /locus_tag="PKN85_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNT43175.1"
                     /translation="MATKETGKKKRTKTKQPAKGVPQPTEQAVVKKSFPIVALGASAG
                     GLEAFEVFFRSLPRTNEAAFVVISHLDPKHASMMSELISRFTRMEVSEAANDMEVGAG
                     HVYVIPPNRNLSISQGRLQLTEQEKSLGMKMPIDAFLRTLADDQGENSVAIILSGTGT
                     DGTLGVRALQAAGGVTFVQDPSEARYDGMPQSAIQTGLADYVLPVEEIARQLSVLFRR
                     YPVGREELPDDAMPNVIQKVLSVVRAKTGHDFSLYKKNTMNRRIRRRMSINNIQDGSD
                     YVRYLREHPEEVEQLFKELLITVTNFFREPEAFDNLKNTILPGIFKDKPEDYTVRVWV
                     PGCATGEEAYSIAIVIREYAEETGRECRLQMFATDMDETSIAQARTGLFPSNIAADVT
                     PARLAKYFVKEEAGYRVRKDIREGIVFATQDVAKDAPFTRLDLVSCRNLLIYMESELQ
                     SKIITIFHYSLKTNGILFLGSSESVGTRTDLFRVIDRKWKYYQAKPASGHEAVLHAAF
                     ASWPPEHVRVDRPSPVVVRKADLEETVRTALLASFAPPAIVVNDKGDILYIYGDTTKY
                     LTPSPGRPSLNITHMTREGLRFPMRSALMAAATHQKEAVYRNVKVKVNGTTMTIDLAI
                     RPLPRAEEEEALFMFTFGEVSEEQAAERTSDERDVDREHLLDLEKELIYTRESLQAAA
                     EEAQAANEELKSANEELQSSNEELQSTNEELETSKEELQSVNEELTTVNAELRSKIEQ
                     LSQSENDMKNLLDSTDIATVFLDNNLNIKRFNSAATKVVSLIPSDAGRPVGDVTMKIE
                     YPDLPNSARDVINRLRPFEAEVRSKEDRWFVMRIVPYRTLDNVIDGVVMTFTDITESK
                     CAADERARFFEHIVQTVREPFLVLNEDLRVVMANRSFLDFFKVRRDETEGRVIRDLGK
                     REWDIPVLRGFLSDVIKTDEVFEDFRVEADFPSIGHHVLVVNARKIKATVASDEPLIL
                     LAMEDFTSKDCFPGETGTGKNPEGKRKSE"
     gene            3677..4024
                     /locus_tag="PKN85_01870"
     CDS             3677..4024
                     /locus_tag="PKN85_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT43176.1"
                     /translation="MKVVRFESAESYEPEKDWKRVSLCAEKDISIEHFVKPPRHASPN
                     HDHPNSQVLIVLKGKLTITTGTEEETLDEGDAVYIPGNEQHVVTNPLDEPSVGIDIFI
                     PGRSFDFWLKRKA"
     gene            complement(4135..4770)
                     /locus_tag="PKN85_01875"
     CDS             complement(4135..4770)
                     /locus_tag="PKN85_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43177.1"
                     /translation="MNSHFTIYTVSWVLYCLLALVMFLRDRRSYAVARREYWRDLVKP
                     WKVVTFLVAAISFMVMAPYTGDPTWDAFDAGFMSLFTFASAPFAVGSLYRMVTGRLPV
                     GQLVVISAVWMFSASWCYDIYIFLRDGSYPVTWSSNITASSLLYFLAGLLWNLDWKKG
                     KGVILSFREETWPYASRERVFGRIVWFALPVMILVAVLMLYFVWTYLRPSG"
     gene            complement(4883..>6486)
                     /locus_tag="PKN85_01880"
     CDS             complement(4883..>6486)
                     /locus_tag="PKN85_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNT43178.1"
                     /translation="TPEYLDSLRKSFRIKFKGHRYDVIIVSDDPALDLMLDLGQDLFP
                     STPVVFCGINNFSDMRLMGKHLYTGVVEEIDIKDTLEAAHALYPKARKVIAPVNSNAT
                     GVSNRRLLQGTIPAFNGIFEFEIVENPELGPFLQRLKSLRRDDYVILLTGRFESSAGE
                     MIPIETSTSEIAKAGIPLYSMWEYYLGHGIVGGMLTNGFYQGKTAAGIARRILNGEKD
                     IPVIRKSPNRYAFDFRQLELFGIAEDLLPAGSVVVNRPVSFYARHADAIHAAVGIVLL
                     CLGIIVFLSIIIVKKNRAERELRRYQDNLEVLVEERTEGLNFANAALTAEIQEREQVE
                     AALRESEEKYRVLIEKANEAVVIVQDETFVFANPRMHDLAGVPAGTLEGRSFVDLIWP
                     DDRDMVMGRYRKRISGESVPDAYDFRLVGPEGAFIWVFLSATTIPWKGRPATLNLLTD
                     ISDRKLAEEERERLITELQEALSQVKALSGLLPICASCKRIRNDEGNWEQMEAYIRDR
                     SEAKFSHGICPECARKLYADQSRKK"
BASE COUNT         1276 a         1971 c         1839 g         1400 t
ORIGIN      
        1 ctgctcttca cgaccctttc gtggtgccga cgatactcgg cggcatagtg ggaatccacg
       61 tactggagga agctcgcgcc gacaaggacc tgcctcaccg tgccgagcaa ggtggcggcg
      121 gtgaggttcg cctcttccac cacgttctgg gcgttgaggg ttaggtagcc cacgggcgca
      181 aaatcgtaga ggtcggccag ccgctgccgc ccctcttcga tgacctgctg ggccttcttg
      241 agttcctcgt tctgcatctc cagttcgacc tggtggaggc gcaggtcttc aaggagtttg
      301 gccgtcttct ccggggaaac gttgcaagcg gttttcaggc cttcggagac atggtccttc
      361 ggaccctttt ctggttcatc ttcccgatgc atcgctgcct cgcctattcg ctcttccttt
      421 tcccttccgg gttctttccg gttcccgtct cgccgggaaa gcagtctttg ctggtaaaat
      481 cttccatggc aagcagaatc agaggctcat ccgacgcgac agttgctttt atcttccggg
      541 cattcacgac caggacatga tgaccgatgg agggaaaatc ggcctcaacc ctgaaatcct
      601 caaagacttc gtcggtcttg ataacgtccg acagaaaccc tctcaggaca ggaatgtccc
      661 actcgcgttt tcccaggtcc ctgatgaccc ggccctccgt ctcgtcccgc ctcaccttga
      721 agaagtcgag aaagctcctg tttgccatga ccacccggag gtcttcgttg agcacgagaa
      781 atggttcccg cacagtctgc acgatgtgct cgaagaatct cgcccgttca tccgccgcac
      841 actttgactc cgtgatgtcg gtgaaggtca tgacgactcc gtcgatcacg ttgtccagcg
      901 tccggtaagg aacgatccgc atgacaaacc acctgtcctc ctttgacctc acttccgctt
      961 cgaagggccg cagtcggttg atcacgtccc gcgcgctgtt gggaagatca gggtattcga
     1021 tcttcatggt cacgtcgccg acaggccggc ccgcatcgga agggatgagg ctgacaacct
     1081 tggtggcagc ggaattgaac cgcttgatat taaggttatt gtcaaggaat accgtagcga
     1141 tatcggtgct gtcgagaagg ttcttcatgt cgttctcgct ctgggaaagc tgttcgatct
     1201 tggacctgag ctccgcgttg accgtcgtca actcctcgtt cacggactga agctcctcct
     1261 ttgaggtctc caactcctcg ttggtggact ggagttcctc gttgctcgac tgcaattcct
     1321 cgttggcgga cttgagttcc tcgttggccg cctgcgcctc ctcggccgca gcctgcaggc
     1381 tctccctcgt gtatatgagc tccttttcca gatccagaag gtgctccctg tcgacatccc
     1441 gttcgtcact cgtcctctcc gccgcctgtt cctcgctgac ttccccgaag gtgaacatga
     1501 agagcgcctc ctcctcttcg gcccttggca ggggacggat ggcaagatcg atcgtcatcg
     1561 tggtcccgtt aaccttgacc ttgacgttcc tgtacaccgc ctccttttga tgcgtcgcgg
     1621 cggccatcag ggccgagcgc atgggaaagc gcaagccctc ccgcgtcatg tgggtaatgt
     1681 tgagcgaggg gcgacccggt gatggagtca ggtactttgt cgtatcgccg tagatataga
     1741 gtatgtctcc cttgtcgttc acgacgatcg ccggaggcgc aaaggatgcg aggagcgcgg
     1801 tgcgcaccgt ctcttccagg tcggccttcc gcaccaccac cggagacggg cggtccactc
     1861 ggacgtgctc ggggggccag gaggcgaagg ccgcgtggag gacggcctcg tgacctgagg
     1921 ccggtttcgc ctggtagtac ttccatttcc tgtcgatcac ccggaaaagg tccgtccggg
     1981 taccgacgct ctcggaagaa ccaagaaaga ggatgccgtt cgtcttgaga ctgtagtgga
     2041 agatggtgat tatcttgctc tggagctccg attccatgta gatgaggagg ttcctgcagg
     2101 atacaaggtc gagcctggtg aagggcgcgt ccttggccac atcctgggtc gcgaagacga
     2161 tcccctccct gatgtccttg cgcacccggt agcccgcctc ctccttgacg aagtactttg
     2221 ccaggcgggc aggggtcaca tccgcggcta tgttggaggg aaagaggccc gtgcgggcct
     2281 gcgctatcga ggtctcgtcc atgtcggtgg cgaacatctg gagcctgcat tcgcggcccg
     2341 tttcctcggc gtactcccgg atcacgatgg cgatggaata ggcctcctcg ccggtggcgc
     2401 agccgggcac ccacacccgc acggtgtaat cctccggttt gtccttgaaa ataccgggaa
     2461 ggatcgtatt cttgaggttg tcaaaggcct ccggttcgcg gaagaagttc gtcacggtga
     2521 tgagaagctc cttgaaaagc tgctccacct cctccggatg ctcccgcaga tagcgaacgt
     2581 aatcggaccc gtcctgtatg ttgttgatac tcatccggcg ccggatcctg cggttcatgg
     2641 tgttcttctt gtagagggag aaatcatgcc ctgtctttgc ccgcaccacc gacagcacct
     2701 tctggatcac gttcggcatg gcgtcatcgg gcagttcttc cctcccgaca ggataccgtc
     2761 gaaagagaac cgagagctgc ctggcgatct cttccacggg cagaacataa tcggcgagac
     2821 ccgtctgtat ggcgctttgc ggcatgccgt cgtatcgcgc ttcagaaggg tcctgaacga
     2881 aggtcacgcc gcccgccgcc tggagggccc tcaccccgag agtcccgtcc gtgcccgtgc
     2941 cggaaaggat gatggccacg gaattctcac cctgatcatc tgccagggtg cgcagaaaag
     3001 catcgatggg cattttcata ccaagggact tctcctgttc cgtgagctgg agccttccct
     3061 gggagatcga caggttcctg ttgggcggga tgacgtagac atgccccgcc cccacctcca
     3121 tgtcgtttgc ggcctcgctg acctccatcc gcgtgaatcg gctgatcagt tccgacatca
     3181 tgctcgcatg cttgggatca aggtgggaga tcaccacgaa agcggcctcg ttcgtccggg
     3241 gaaggctcct gaagaacacc tcgaacgcct caagcccccc tgccgacgcg ccaagggcca
     3301 ctatgggaaa ggatttcttc acaacggcct gctccgtcgg ttgcggcacc cctttcgctg
     3361 gctgcttagt ctttgtgcgt ttcttcttgc ctgtctcttt ggttgccatc atcacccccc
     3421 tgagtgaccg tcttctgcag aaacatccgg acggtcaacc caacaatgat aacacaagta
     3481 taaacgcctt tacgggatat agcaatcacc gctcgaacca catagatctc gaacagaagc
     3541 gccctggcgg gacagccctc aaggcggcac ctttttccat tgacctcttc ccgcgatgag
     3601 acgaaaatga aggtgtggaa cttgaatcgt gcgtggcagg ggctttaagt ccctgaaaac
     3661 agcaaggagg cctggaatga aggtagttcg ttttgaaagt gccgaaagct atgagccgga
     3721 aaaggactgg aagcgggtga gcctctgcgc ggaaaaggat atctccatcg aacacttcgt
     3781 gaaaccgccg cgtcacgcgt cgccgaacca cgaccatcca aactcgcagg tgttgatagt
     3841 cctgaagggc aagctcacga tcaccaccgg cacagaggaa gagacactgg atgagggcga
     3901 cgcggtctac atccccggga atgaacagca cgtcgtcacg aatcccctcg acgaaccgtc
     3961 cgtcggcatc gacatcttca tcccgggacg gtccttcgac ttctggctca aacgaaaggc
     4021 gtgacaaggc ggatgatctt tccactattt gggaaaagga tcggtcccgg ccctctcagg
     4081 aagccggacc agcgggcagc acgcaccccc gctcgcaggc ctgaggatcg acgctcaacc
     4141 ggaagggcgc aggtaggtcc agacgaaata cagcatgagc accgcgacga ggatcatcac
     4201 gggaagcgcg aaccagacga tcctgccgaa taccctttcc cgggacgcgt atggccacgt
     4261 ctcttcccgg aacgacagta tcactccttt ccctttcttc cagtccaggt tccacaggag
     4321 cccggcaagg aaatagagca gggaggatgc cgtgatgttc gaggaccacg taacggggta
     4381 actcccgtcg cgaaggaaga tgtatatgtc atagcaccag ctcgccgaga acatccagac
     4441 cgcggatatg acgacgagct gtccgacagg cagccttccc gtaaccatcc tgtacagcga
     4501 ccccacggcg aagggggcgc tcgcgaaggt gaacagggac atgaaacctg cgtcgaaggc
     4561 gtcccatgtc ggatcgcccg tgtagggagc catgaccatg aaggatatgg ccgcaacgag
     4621 aaaggtgacg accttccatg gtttgacgag gtcccgccag tattcccgcc gggctaccgc
     4681 gtaagaacgc ctgtcccgga ggaacatgac gagcgccaga aggcaataca ggacccagga
     4741 taccgtgtat atggtgaaat gagagttcat ccctgtcagc ttcacccgtt cgggtcattt
     4801 tttcaagcgg tttccgagga tcccgccaca cggggcggcc gacctgcgga tcactgccat
     4861 catcgggggg aagagggaag gctcatttct tcctggactg gtctgcgtag agcttcctgg
     4921 cacactcggg gcagatgccg tgggagaact tcgcttccga ccggtccctt atgtacgcct
     4981 ccatctgctc ccagttgccc tcgtcgttcc ttatcctctt gcacgaggcg cagatgggga
     5041 gcaacccgct caacgccttg acctgggaaa gggcctcctg aagctcggtg atgagccttt
     5101 cccgctcctc ctccgcgagc ttgcggtccg atatgtccgt cagcaggttg agtgttgccg
     5161 gcctgccttt ccacgggatc gtggtggcgg acaggaaaac ccagatgaat gcgccttcgg
     5221 ggccgacgag cctgaaatcg tacgcgtcgg gaacgctttc gccgctgatc cgcttcctgt
     5281 acctgcccat gaccatgtcc ctgtcgtcgg gccagatgag atcgacaaag gacctgccct
     5341 ccagggtccc ggccggtacg cccgcgagat cgtgcatcct ggggttcgca aaaacgaagg
     5401 tctcgtcctg cacgatgacg accgcttcgt tcgccttctc gatgagaaca cggtatttct
     5461 cctcgctctc ccgaagcgcc gcctcgacct gttcgcgctc ctgtatctct gccgtcagtg
     5521 ccgcgttggc aaaattgagc ccctcggtcc tttcctccac caggacctcc aggttgtcct
     5581 ggtagcggcg cagctccctc tcggccctgt tcttcttgac tattatgatc gacaggaaga
     5641 ctatgatgcc gaggcataaa aggacgatcc cgacagcggc gtgaatggca tcggcgtgcc
     5701 tggcatagaa cgacaccggt ctgttgacga cgacgctccc ggcaggcagc aggtcctccg
     5761 cgatcccgaa aagctcgagc tgcctgaaat cgaaggcata ccgattcggg ctcttcctga
     5821 tcaccgggat gtccttctcg ccgttcagta tcctccgggc gatcccggcc gccgtctttc
     5881 cctgatagaa accgttggtc agcattccgc ccacaatacc gtggcccaga taatactccc
     5941 acatgctgta gagcgggatc cccgccttcg cgatctcaga agtgctcgtc tctatcggta
     6001 tcatctctcc tgccgacgat tcgaacctcc ccgtgagcag gatgacgtag tcatcccttc
     6061 gcagactttt caggcgctga aggaagggtc caagctcggg gttctcgact atctcgaact
     6121 cgaagatgcc attgaacgcg gggatggtgc cttgcagcag gcgcctgttc gaaactcccg
     6181 tcgcgttgga gttgacggga gcgatcactt tgcgggcctt cggatacagg gcgtgggccg
     6241 cctcgagagt gtccttgatg tctatctctt cgacaacacc cgtgtagaga tgtttcccca
     6301 tcaatctcat gtcggagaag ttgttgatcc cgcaaaacac aacgggtgtt gatggaaaaa
     6361 ggtcttgccc aaggtccagc atcaggtcga gggccggatc gtcggagacg atgatcacgt
     6421 cgtatcgatg acccttgaac ttgatgcgga aagacttgcg caggctgtcc aaatactccg
     6481 gagtga
//
LOCUS       DAOQTR010000044         6394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13382_length_6394_cov_3.695536, whole genome shotgun sequence.
ACCESSION   DAOQTR010000044 DAOQTR010000000
VERSION     DAOQTR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13382_length_6394_cov_3.695536"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="PKN85_01885"
     CDS             <1..1345
                     /locus_tag="PKN85_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNT43179.1"
                     /translation="AFMKEMKDMMAYINFRNTNPIPDVMTILSGIGSPQEMVHTDEKW
                     HTENFMWGNARTRRRGFWDEKINEEWPRTQLEGIKRLISCFNCPQHCGALVAYKDTPR
                     YMAKCFGKLTYAMAAYVDSMDFAWRTLQKATEYGVDSFSTPQILAFAVELIEAGILTE
                     KDLKAGPTWPACPTDKEGRFLWLLDRVAHREGIGEILADGVYLAARQIGNGAEAFDHN
                     TIKRHEQLPLKLGTMDPLYYLMYATNEKISITQIEGNWPQGAFPTMEQREAFVKDWPQ
                     LPDDSWKQWLLEWEPRGEKAIPYFPTIDMCCHIVDWMEMLHNIDDACCVCAGMSSFCL
                     KPPYHIHNYPRIISAATGLDLDEASLKKIVNRSRNLHRALNNRRGMSRKDEKPPQDHW
                     KRRFPEIEENLMSAYYTFKGWNQDGVPTKERLNELDLGYVAEDLEKRGLLKDGQD"
     gene            1332..1871
                     /locus_tag="PKN85_01890"
     CDS             1332..1871
                     /locus_tag="PKN85_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43180.1"
                     /translation="MAKIKKKIKTIKVDADKCNGCRGCEIVCSAFHSTPRYSTINPAR
                     SRIQIIRHPLKDVWLPIFAGEYTPAECMGREKYVIDGKEYDECTFCRATCPSRDIFKE
                     PDSGLPLKCDMCEDEPPLEQPKCVEWCLNDVLVYEEREEEVEESVEMEKVETGLVSMI
                     DKYGLDKVAEAIARISQKD"
     gene            1902..3053
                     /locus_tag="PKN85_01895"
     CDS             1902..3053
                     /locus_tag="PKN85_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNT43181.1"
                     /translation="MEIIAPFQEVIDEIKEKGGDAVKYCYQCGKCDTVCPWNKVRKFS
                     MRKLIREATFGISEIEKEEIWRCTTCGKCPQRCPRDVKQIDDMMAIRRMATGYGVFAG
                     PVKPYRTVASGLSGQGNPFNEDRKTRAAWADGLGVKPYKEDMEYLYFPCCYCSYDPRL
                     KKVAQATAKVLKKAGVSFGILGEKENCCGESIRKTGNEDLFKKLAKENIKAFVDAGVK
                     KILVSSPHCYHTFKNEYPMFKVNFEVVHISQLLPQLIAEGKLTLSGEYAKKVAYHDPC
                     YLGRHNGIFDDPRDALKAVPGVELTELPEARMDSTCCGMGGGRVWAETEKHERFSNIR
                     VEQALEVGAKELITACPYCITALEDSRLVMNHADDIEVKDITEVILEVM"
     gene            3087..6230
                     /locus_tag="PKN85_01900"
     CDS             3087..6230
                     /locus_tag="PKN85_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3315..3317,aa:Sec)
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43182.1"
                     /translation="MENVISLNENIKELCKSRAAGNFGDVMVVGGGISGIQASLDLAT
                     AGFRVYLVEKAPSIGGHMAQLDKTFPTNDCSMUILAPKLVEVGRHPNIEVLTYTEVEN
                     VEGEAGNFNVTLLRKPRYIKEDKCTGCTVCVEYCPVKYPDQYNQDISLNKAVHVYFAQ
                     AIPLITYIDESCLYLKEKKCRICEAVCKNDAIDLNQQPVKEDINVGAIILAPGFEPFD
                     PKVREEYRYGEFQNVVTSMDFERLLCSTGPYAGEILRKSDLKHPHNIAWIQCIGSRQV
                     IEGGNSYCSAVCCTYTQKQVILTKDHDADAKCTIFHNDVRAYGKDFERYYERTQNLPG
                     IRFFRSYPSIVKEDPVTHNVTIRYSTPEDGVIEEEFDMVVLSIGMNPPKGVEDMAKQY
                     GFELEEHDFCKIDPRNPMLTNRPGIFVSGALQGPIDIPESVFSASGAGSQIGEMLQYR
                     RGNLAKERIYPEEKDVSQEEPRVGVFVCHCGANIGRIVNVPGTVEYCKTLPNVVYAQE
                     QLFSCATNSAREITDKIKEEGLNRVVVAACSPRTLEPLFRDTLREAGINQYYYEMANI
                     KEHNSWVHQKEKEEATEKAHDIIRMSVARACQLEPLQEFDLPVDKRALVVGGGIAGMN
                     CALSIANQGHEVHLIEREKELGGIANRIHTTLQGLDVQEYVKDLKSKIYQHPLVHVYH
                     NAKITEATGYVGNFVTTVESNKGTSSIKHGASVIAIGIDMYTPTEYLYGQDERVLTHL
                     ELEEKITSDDEKVTGAQSLVMIQCVGCRNEERNYCSRICCSESIKNALLLKDKNPDMD
                     IYILFRDVRTYGFNEDYYRLAAERGVKFIRYEPEDPPVVEPGKAEDKRDVLKVTATDY
                     ILGKKLELDADIIALAAAVVPSAATKEVANQFKVTLSPDGFFKEAHVKLRPVEFATDG
                     VYLCGLAHYPKFIQETINQSYGAAGRVLTLLSNDIVVASGSVCAVNEKQCMGCGACVE
                     VCTYGALELKETKQGKKCVVNPVLCKGDGLCNTKCPTGAIQLRHYTDTELTCEIDVVV
                     PDDEIMKQIDAAAAADA"
     gene            6255..>6394
                     /locus_tag="PKN85_01905"
     CDS             6255..>6394
                     /locus_tag="PKN85_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6306..6308,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT43183.1"
                     /translation="MSTAKFKPRVLGFACNWUAYGAADLAGVSRLQYTTEMRIIRVMC
                     SG"
BASE COUNT         1610 a         1787 c         1888 g         1109 t
ORIGIN      
        1 agccttcatg aaggaaatga aggatatgat ggcctacatc aacttccgca acacgaaccc
       61 catcccggac gtcatgacga tcctctccgg tatcggttcg ccgcaggaaa tggtgcacac
      121 cgatgagaaa tggcacaccg agaacttcat gtggggcaac gcgcgtaccc gcaggagagg
      181 tttctgggac gagaagataa acgaagaatg gccgagaacc cagttggagg gcatcaagcg
      241 gctcatcagc tgcttcaact gtccccagca ctgcggcgct ctggtcgctt acaaggacac
      301 cccgcgttac atggccaagt gtttcgggaa actgacatac gccatggcgg catacgtgga
      361 tagcatggac ttcgcctgga ggacgctgca gaaggcaacc gaatatggcg tcgactcctt
      421 ctcgaccccg cagatcctgg cctttgccgt cgaactcatc gaagcgggta tcctcaccga
      481 gaaggacctc aaggcgggcc ccacgtggcc ggcatgcccc acggacaagg aaggaaggtt
      541 cctctggctc ctcgacaggg tcgcccaccg ggaaggcatc ggcgagatcc tggcggacgg
      601 cgtttatctc gcggccaggc agatcggcaa cggcgcggag gccttcgatc acaacacgat
      661 caagagacac gagcagctcc ctctcaagct cggcacgatg gatcccctgt actacctgat
      721 gtatgcaacc aacgagaaga taagcatcac acagatcgag gggaactggc ctcagggcgc
      781 cttcccgacg atggagcaga gggaggcctt cgtgaaggat tggccgcagc ttcccgatga
      841 cagctggaag cagtggctcc tcgaatggga accccgcggg gaaaaggcga taccctattt
      901 cccgacgatc gatatgtgct gtcatatagt cgactggatg gagatgcttc acaatatcga
      961 tgacgcctgt tgtgtctgcg ccggcatgtc atccttctgt ttgaagcctc cctatcatat
     1021 acacaactac ccgaggataa tctcggcggc gacgggtctc gacctcgacg aagcctcgct
     1081 gaagaagata gtcaacagaa gccgcaacct gcacagggcg ctcaacaaca ggcgcggcat
     1141 gagcagaaag gacgagaagc cgccacaaga ccactggaag agaaggttcc ccgagataga
     1201 ggagaacctc atgtccgcgt attacacgtt caagggctgg aaccaggatg gcgttcccac
     1261 aaaggaacgg ctcaatgagc tggatctcgg ttacgttgcg gaagacttag aaaagagagg
     1321 tttattgaaa gatggccaag attaagaaga aaatcaagac gatcaaggtt gatgccgata
     1381 agtgcaacgg ctgccgggga tgcgagatag tctgttccgc ctttcactca acgcccaggt
     1441 acagcacgat caatccggca cggtcccgca ttcagataat ccgtcacccg ctgaaagacg
     1501 tatggcttcc catattcgcg ggcgaatata ccccggcgga atgcatgggc agggaaaagt
     1561 acgtgatcga cggcaaggaa tacgacgagt gcaccttctg cagggccacc tgcccgtcga
     1621 gggacatatt caaggaaccc gactccgggt tgcctctcaa gtgcgacatg tgcgaggacg
     1681 agccgcccct cgaacagccg aaatgcgtcg agtggtgcct caatgacgtc ctcgtgtatg
     1741 aagagaggga agaagaggtc gaggagagcg ttgagatgga aaaggtcgag acgggccttg
     1801 tatccatgat cgacaagtat gggctggaca aggtcgcgga ggccatagcc cggatatccc
     1861 agaaggatta gacgtcaaga taacttaaag aggatggata cgtggaaatc atagccccct
     1921 ttcaagaggt aatagacgag atcaaggaga aaggcggaga cgccgtcaag tactgttacc
     1981 agtgcggcaa gtgcgatacc gtctgtccct ggaacaaggt gcgcaagttc agcatgagaa
     2041 agctcatccg cgaggccacc ttcggcatca gcgagatcga gaaggaagag atctggcgct
     2101 gcaccacctg cggcaagtgc cctcagcgct gccccaggga cgtcaaacag atcgacgaca
     2161 tgatggcgat ccgcaggatg gcgacggggt acggcgtgtt tgccggtccg gtcaagccct
     2221 accggacggt cgcctcgggc ctttccggcc agggcaatcc cttcaacgaa gaccgcaaga
     2281 ccagggccgc ctgggccgac ggcctcggcg tgaaaccgta caaggaagac atggagtacc
     2341 tctatttccc gtgctgctat tgcagctacg acccgaggct caagaaggtc gcgcaggcca
     2401 cggcgaaggt cctgaaaaag gccggcgtca gcttcggtat cctcggtgaa aaggagaact
     2461 gctgcgggga aagcatccgg aagacgggta acgaggacct cttcaagaaa ctcgcgaagg
     2521 agaacatcaa ggccttcgtc gatgcgggcg tcaagaagat ccttgtttcc tcccctcact
     2581 gctaccatac attcaagaac gaatatccca tgttcaaggt caacttcgaa gtggtgcaca
     2641 tctcccagct cctgccgcag ctcatcgcgg aagggaagct cacgctctcg ggagagtacg
     2701 ccaaaaaggt cgcctatcac gacccctgct acctcggccg gcacaacggc atcttcgacg
     2761 acccccggga cgcgctgaaa gcggttcccg gcgtcgagct caccgaactg cccgaggcac
     2821 gcatggacag cacctgctgc ggcatgggcg gaggaagggt ctgggcggaa acggaaaagc
     2881 acgagcgctt ttccaatatc agggtagagc aggccctcga ggtgggcgcg aaagaactca
     2941 tcaccgcctg cccgtactgc atcaccgcgc ttgaggactc aaggctggtc atgaaccatg
     3001 ccgacgatat agaggtcaag gacataacgg aagtcatcct ggaagtgatg tagcatacaa
     3061 gaaaaaaacc gatgaggtga tagaaagtgg aaaacgttat atcgctcaac gaaaacatta
     3121 aagagttgtg caagagccgc gcggccggca actttggcga cgtgatggtc gtcggcggcg
     3181 gcataagcgg catccaggcc tcactggacc tcgccacggc gggattcagg gtatacctgg
     3241 ttgagaaggc gccgtcgatc ggcgggcaca tggcccagct cgacaagacc ttcccgacca
     3301 acgactgctc gatgtgaatt ctcgcgccta agctggtcga ggtcggccgg catccgaaca
     3361 tcgaagtgct cacctacacg gaagttgaga atgtggaggg agaagcagga aatttcaacg
     3421 taacactttt gcggaaaccc cgctacatca aagaggacaa gtgcacgggc tgcaccgtct
     3481 gcgtggagta ctgcccggta aagtacccgg accagtacaa ccaggacata tcgctgaaca
     3541 aggcggtcca cgtctatttc gcgcaggcca tcccgctcat cacctacatc gacgagagct
     3601 gtctctatct caaggagaag aaatgccgca tctgtgaggc ggtctgcaag aatgacgcga
     3661 tagatctcaa ccagcagcct gtgaaggaag atatcaacgt cggcgccatc atcctcgccc
     3721 ccggtttcga gcctttcgat ccgaaagtga gagaggagta ccgctacggc gagttccaga
     3781 acgtcgtgac gagcatggac ttcgagaggc tcctgtgttc caccggccct tacgccggcg
     3841 agatcctccg caaatccgac ctgaagcacc cccacaacat cgcctggata cagtgcatcg
     3901 gctcccgcca ggtgatcgag ggcggcaaca gctactgctc cgcggtctgc tgtacctaca
     3961 cccagaaaca ggtgatcctg acgaaggacc acgacgcgga cgcgaaatgc accatctttc
     4021 acaacgacgt gcgcgcctac ggcaaggatt tcgagcgcta ctacgagagg acgcagaacc
     4081 ttccgggcat caggttcttc aggagctatc cgtccatcgt gaaggaagac ccggtgacgc
     4141 acaatgtcac catacggtac agcacccccg aggacggcgt catcgaggaa gagttcgaca
     4201 tggtcgttct ctccatcggc atgaacccgc cgaagggtgt ggaggatatg gcgaagcagt
     4261 acggcttcga gctcgaggag catgatttct gcaagatcga tcccaggaac ccgatgctga
     4321 cgaacaggcc gggcatcttt gtgagcggcg ccctccaggg gcccattgat attcccgagt
     4381 ccgttttcag cgcctccggc gccggttccc agatcggcga aatgctccag tacagaaggg
     4441 gcaacctggc gaaggaaagg atctaccccg aggagaagga cgtatcccag gaagagccca
     4501 gggtcggcgt tttcgtctgc cactgcggtg ccaacatcgg caggatcgtc aatgtcccgg
     4561 gaacggtcga gtattgcaag accctcccca acgtcgtcta cgcccaggaa cagctcttct
     4621 cctgcgcgac aaactcggcg agggagataa cggacaagat aaaagaagaa ggattgaacc
     4681 gcgtggtcgt cgcggcttgt tccccgagga ccctggagcc tctcttcaga gatacgctcc
     4741 gcgaggccgg gatcaaccag tactactacg agatggctaa catcaaggaa cataactcct
     4801 gggtccatca gaaggagaag gaagaggcca cggagaaggc ccatgacatc atcaggatgt
     4861 ccgtggcacg cgcctgccag ttggaacccc tccaggagtt cgacctgccc gtggacaaga
     4921 gggcgcttgt cgtcggcggc ggcatcgccg gcatgaactg tgccttgtcc atagcgaatc
     4981 agggccatga ggtccacctc atagagaggg agaaggaatt ggggggcatc gcgaacagga
     5041 tccacacaac gcttcagggc cttgatgtcc aggagtacgt gaaggacctt aaatcgaaga
     5101 tctaccagca tcccctggtt cacgtgtatc acaacgcgaa gatcacagag gcgacaggtt
     5161 atgtcggaaa ctttgtgacc acggtggagt ccaacaaggg gaccagttca ataaagcacg
     5221 gtgcatcggt gatcgccatc ggcattgaca tgtacacgcc cacggaatac ctctacgggc
     5281 aggatgaaag ggtccttacc catctggaac tggaagagaa gataacctcc gacgatgaga
     5341 aggtcacggg cgcgcagagc ctcgtgatga tccagtgcgt gggctgcagg aacgaagagc
     5401 gcaactactg cagcaggata tgctgcagtg aatcgatcaa gaacgccctc ctcctcaagg
     5461 ataagaaccc cgacatggac atctacatcc tcttcaggga tgtgaggact tatggattca
     5521 acgaggacta ctaccgcctg gcagccgaga gaggcgtgaa gttcatccgt tacgaaccgg
     5581 aagacccgcc tgtggtggaa ccgggcaagg ccgaggacaa gagagacgtc ctgaaggtca
     5641 cggcgacgga ttatatcctg ggcaagaagc tcgaacttga cgcggacatc atcgcgctgg
     5701 cggcagccgt cgttccttcg gcggcgacaa aggaagtcgc gaaccagttc aaggtcacct
     5761 tgagccccga cggcttcttc aaagaggcgc acgtgaaact gaggcccgtc gaattcgcga
     5821 cggatggtgt ctacctctgc ggcctcgcgc actacccgaa gttcatccag gagacgatca
     5881 accagtcata tggcgcggcc ggaagggtcc tcacccttct gtccaacgac atcgtcgtgg
     5941 cctccggttc cgtctgcgcc gtcaacgaga agcagtgcat gggttgcggg gcatgcgtcg
     6001 aggtctgtac ctatggtgcc ctggagctca aggagacgaa gcagggcaag aagtgcgtgg
     6061 tcaaccccgt gctgtgcaaa ggggacggcc tgtgcaacac caagtgtccc accggggcga
     6121 tccagcttcg ccattacacg gacaccgagc tcacatgtga gatcgatgtg gttgtccccg
     6181 acgacgagat aatgaaacag atcgacgcgg cagcggccgc agatgcgtaa acctatagaa
     6241 ggaggtggga acggatgagt acggcaaaat tcaaaccgag agtccttggc ttcgcatgca
     6301 actggtgagc gtacggcgcc gctgacctgg ctggagtttc cagactgcaa tatacaactg
     6361 aaatgaggat catccgcgtc atgtgttccg ggag
//
LOCUS       DAOQTR010000045         6378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13430_length_6378_cov_3.228373, whole genome shotgun sequence.
ACCESSION   DAOQTR010000045 DAOQTR010000000
VERSION     DAOQTR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13430_length_6378_cov_3.228373"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKN85_01910"
     CDS             <1..687
                     /locus_tag="PKN85_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT43184.1"
                     /translation="GSYVFYYRELEEEAPIPFEERILYRDEHILAVDKPHFLPVVPSG
                     RFLRETLLVRLKKDLSLEHLVPIHRIDRETAGVVIFSHDPATRGRYASLFQEQKVEKV
                     YEALAPTLPGMSFPFVHRSRLKEGRPFFRMEEVEGPANSETRIEVIEIKGDIALYRLI
                     PATGRKHQLRVHMAALGIPIINDRFYPDAGPAGNDDILRPLKLLARSVSFPDPLTGRN
                     MRFESTRELS"
     gene            706..1143
                     /locus_tag="PKN85_01915"
     CDS             706..1143
                     /locus_tag="PKN85_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNT43185.1"
                     /translation="MKEHLEIVRRQFEKDEYARSLGIVLDDLTDTTIRMHMPLEPGMM
                     NFYGRPHGAVIYSLADAAFSVLGNNRNTICVALDCSITYHASPDPKAILVVEGDTLSL
                     NRRTAAYVFNVYSDDGALRTLIATMKSVSYRTGRPIEPGIHQE"
     gene            complement(1158..1898)
                     /locus_tag="PKN85_01920"
     CDS             complement(1158..1898)
                     /locus_tag="PKN85_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNT43186.1"
                     /translation="MASTTRTGGVFRYKVGQLRRFHVLWVVPAFLFLAACSVVKAPYY
                     VVKGTIKGGYYAVKGAYELTSGTTKIVYTIGKYTFKITKAPLDWAFANEDIEDIDGLP
                     PREAIRKGRVKTAPYTVKGKRYYPMSVEQAKSYDETGIASWYGYEALRHNPSRMTANG
                     EVFDPKQMTAAHKYLPLPTHVKVTNLENNRSVIVRVNDRGPFPSDNNPSSGQRIIDLS
                     AGAAKKLGFYNKGLARVRVETIELEREG"
     gene            2233..3321
                     /locus_tag="PKN85_01925"
     CDS             2233..3321
                     /locus_tag="PKN85_01925"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT43187.1"
                     /translation="MNSRHRRNAAKTLRYFIAFTICITFLILIIPGLSRAERPLPSDT
                     RKDHGYPAPDTQGTKNSVPTVDDWKKFGEQLRSRAPADELQKARFVAAEVAKELDRKG
                     ITVNTSLIGRIGASLKYGNSEAATCGYLNDAVTASLKGAGFDAGQIHSVVGYKDGWQA
                     LNRGYIFDVNINHVAPVVVIDGAPYSFDLWAHGGSEGSFKDFGNSAWNGIKTESWGEL
                     MRKHQGYNAFSSDEGITKKDLAEANEELVEKTKANLKAMRIEVRDSYGKPVSGARVTL
                     THETRYSGTTGMDGSVTAKGVKPAKYAVRVTAEGYLTFSDTAELAPMRDDRYSVTLEP
                     ILSSVVATVRSGSIPVVDAKVRMSSRSP"
     gene            3427..4890
                     /locus_tag="PKN85_01930"
     CDS             3427..4890
                     /locus_tag="PKN85_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43188.1"
                     /translation="MKPKDEKSAATPVPCNFNLKPRITVRVKGAGQAFTGDVVELEAE
                     VEATESIKPALKYAWRLVGDNTVLASTAKFKRTMVHPGTYVVSIVVYVHRPGVGNMLV
                     GEAVHKIVAEARRITVSLSGPGESKVGDDVMLRAQIKQLSDSDKEATYTFGWSVNGMK
                     FGGNGETQSLKVTQAGRNTVRAVVWQAVGGKWVRAGDASHAFVAAGPDPAKGSVSVSG
                     PSSVEVGDRIDLNASVGDTNVPASALYYSWVVNGRRYNYRESIALPASAPGTYNVTAE
                     LWMKYQPKPVKLAQAFHTVTIVKKGEKPDPLGDLMAAILGGSDKPGTSTGASPTKPDT
                     KVPVGRAYTAAKLPANQFSLSGTWNAVDSKGRFNGKMSLSQSGSSVSGTMQTPGGGIP
                     VSGSISGSTVSVILTFGSPAIINQYLQDMRVSQAVGSITAKATFTIGSNNNELQGTLY
                     PFHVQWNDDGKTVIVKRKWQGGETHPGNPPRSFTLRR"
     gene            5051..6223
                     /locus_tag="PKN85_01935"
     CDS             5051..6223
                     /locus_tag="PKN85_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43189.1"
                     /translation="MNPTDLLAQSAKLVIQVEEQAVAVVTAIAFFMGSMRTIVTLYRK
                     GYENIIPYLKDQTGRFMIICALCTPLPFLGGTDGKAGTFIGTFPRMVISAGFAQAGNE
                     KVFDNLKAVKAKLGKEMGSGELSEKLTNIKEASPLTGVKIKWVDAIKVRLAQLIFLLP
                     LIPPSSCLALFHPALGAFMMMISYVASEYMAVITCGIGVNVDTSSMVFVLETVRELVL
                     SHMGAITGFLFHTLLVFTFTGVIIAAAVRAAVFCVTFPYSIITMAFDSRREVFFQGIV
                     RAFAIALTPVVAVNVLNVLAMAYDLFMSGGLAGGMIEAYLFSNIDSLVDSGVFPVAGE
                     MYGFVFRLFIATVLAPSVLSIPAIVFLIGSYRVASEAIGAGLSYSGGMGRGGGGGE"
BASE COUNT         1326 a         1843 c         1954 g         1255 t
ORIGIN      
        1 gggagctacg ttttctacta ccgtgaactg gaagaggagg ccccgatccc ctttgaggag
       61 aggatcctct accgggacga gcacatcctg gcagtcgaca agcctcattt ccttcccgtg
      121 gtgccctcgg gcaggtttct ccgggagacc ctcctcgtcc ggctgaagaa ggacctctct
      181 cttgagcacc ttgtccccat tcaccgtatc gacagggaaa cggcgggggt ggtcatattc
      241 tcccacgatc ccgctaccag gggcaggtac gcgtcgctct tccaggagca gaaggttgaa
      301 aaggtctacg aggcgcttgc gccgaccctt ccgggtatga gctttccttt cgttcatcgc
      361 agccgcctca aggagggcag gcccttcttt cggatggagg aggtcgaggg gccggcaaac
      421 tcggaaacgc gcatcgaggt cattgagatc aagggagata tagccctcta ccgtctcatc
      481 ccggcaacgg gccggaaaca ccagctgcgg gtgcatatgg ccgctctcgg catcccgatc
      541 atcaacgaca ggttctatcc cgacgcgggg cccgcgggaa acgatgacat cctgcggccc
      601 ctcaagctgc tggcccggtc cgtttccttc cctgacccgt tgacgggaag gaacatgcgc
      661 tttgagagca cgagagagct gtcgtgacgc gggaggtggc gagcgatgaa ggagcatctc
      721 gagatcgtac gaaggcagtt tgagaaagat gaatacgcgc ggtccctggg catcgttctc
      781 gatgacctga cggataccac gatccggatg cacatgcccc ttgagcccgg catgatgaac
      841 ttctatgggc ggcctcacgg ggccgtcatc tactccctcg ccgatgctgc cttttcagtc
      901 ctggggaaca acaggaacac catctgcgtc gccctcgatt gttccatcac ctaccacgca
      961 agccccgacc cgaaggcaat acttgtcgtc gagggagaca cgctctcgct gaaccggcgg
     1021 acggccgctt acgtcttcaa cgtgtactca gatgacggcg ctctccgcac tctcatcgca
     1081 accatgaaga gcgtgtccta caggacggga cggcccatcg aacccggtat ccatcaggaa
     1141 taggacctcc cgggaggtca gccctccctt tccagttcta tcgtttcgac ccgtacccgt
     1201 gccagccctt tgttgtagaa cccgagtttt ttcgcggcgc cagcggacag gtcaatgatc
     1261 ctttgtcctg acgaggggtt gttatcgctc ggaaagggtc ccctgtcgtt gaccctcacg
     1321 atgacggaac ggttgttctc gaggttcgtg accttcacgt gggtcgggag cggcaggtat
     1381 ttgtgcgccg ccgtcatttg cttcgggtcg aacacctctc cgttggcggt catgcgcgag
     1441 ggattgtggc gcagggcctc gtagccgtac catgaggcta ttcccgtctc atcgtaggac
     1501 ttcgcctgtt ccacggacat gggatagtat ctttttccct ttaccgtata cggcgcagtc
     1561 tttacccggc ccttgcggat cgcctccctg ggcggcagac cgtcgatgtc ctcgatgtct
     1621 tcgttggcga aggcccaatc cagcggcgct ttcgtgatct tgaaggtgta ctttccaatg
     1681 gtgtaaacga tcttcgtcgt gcccgaggtg agctcgtagg ctcccttaac ggcataatag
     1741 ccgcccttta ttgtcccctt gacgacgtaa taaggcgcct tgactaccga acaagcggca
     1801 aggaaaagaa aagccggaac gacccatagg acatgaaacc tgcgcagttg cccaactttg
     1861 tagcggaaaa ccccgcctgt ccgggttgtc gaggccatac tgataaagag cctagcatat
     1921 tcggtcgaag aatagtacac gcaccggatg aaaaagaatg caggcagatc aaggcgtcgc
     1981 ggggagtgaa agggataata ttgacggatt ccgacgtgta ggttaaataa ttgtgtccgg
     2041 ggctgccgga atcctctccg gcccagaccg tcttgttcgc atgttatgtt cctttctgcc
     2101 cgcgaccctg tggtcgtctg gccgcatcga tcggcgcccc gttgaggtct tgacgctcta
     2161 ccgatataga ttccccttga tcgaagcatt gagacggcga tcaggagaca cgccgcgaga
     2221 aggagccgag caatgaatag ccgccacaga aggaacgccg cgaagactct gagatatttc
     2281 atcgccttca ccatctgcat cacatttcta atcctgatca taccgggatt gtcccgcgcc
     2341 gaaagaccct tgccgagcga tacccgcaag gaccacgggt atccggcgcc ggatacacag
     2401 ggtacgaaga actcggttcc gaccgtcgac gattggaaga agttcggaga gcaactccgg
     2461 tcaagagcgc cggcggacga gcttcagaag gcgcgctttg ttgctgccga ggtggcaaag
     2521 gagctggaca gaaagggtat caccgtcaac acgtctctta ttggccgaat cggagcgtcg
     2581 ctgaaatacg gcaacagcga agcggccacg tgcggctatc tcaacgacgc cgtcacggca
     2641 agcctgaagg gggctgggtt cgatgccggc cagatacatt ccgttgtggg atacaaggac
     2701 ggctggcagg cgttgaaccg gggttacatc ttcgacgtca acatcaacca tgtcgcgcct
     2761 gttgtcgtta tcgacggcgc accatactcc ttcgacctct gggcgcatgg aggttccgag
     2821 ggaagtttca aggatttcgg caacagcgcg tggaacggca taaagacgga aagctgggga
     2881 gagctgatgc gcaagcatca gggctataac gccttttctt ccgatgaggg gatcacaaag
     2941 aaagacctcg cggaggcaaa cgaggagctt gtagaaaaga cgaaggccaa tctgaaggcg
     3001 atgaggatcg aggtcaggga cagctacggc aaacccgtga gcggcgcgcg ggtgacgttg
     3061 acacatgaga cgaggtacag cgggacgacc gggatggatg gttccgtcac ggcaaagggg
     3121 gtcaagccgg caaaatacgc ggtccgggtg acggcggaag ggtacctgac cttttcggac
     3181 accgcggaac tcgcgccgat gagggacgac aggtactccg tgacactgga gccgatcctg
     3241 agcagcgtcg tcgccacggt gaggtcgggt tccatacctg ttgtggacgc gaaggtccgc
     3301 atgtcgtccc ggtcccccta gactacgggc gaagcgggcg atgccctctt cgagaaggtc
     3361 cccccgggga catacaccct caccgccgag gccgaggggt tcgcggagga gacacggaag
     3421 atcacggtga aaccaaagga cgagaagagc gccgcgacac cggtcccgtg caatttcaat
     3481 ctcaagccgc ggatcacggt cagggtcaag ggggcaggac aggccttcac cggcgatgtc
     3541 gtcgagctcg aggccgaggt ggaggccacg gagtccatca agcccgccct caagtacgcg
     3601 tggcgccttg tcggcgataa cacggtgctt gcctccaccg ccaaattcaa acggaccatg
     3661 gtccatccgg gtacctacgt ggtgagcatc gtcgtctacg tgcaccgccc tggcgtgggg
     3721 aatatgcttg tcggagaggc cgtccacaag atcgtcgccg aagcgcggag gatcaccgtg
     3781 agcctgagcg gccccgggga gagcaaggtg ggcgacgatg tgatgttgag ggcgcagata
     3841 aaacaactca gcgacagcga caaggaggcg acgtacacct tcggctggtc ggtgaacggt
     3901 atgaagttcg gaggcaacgg tgaaacgcag tctctgaagg tgacacaggc cggccggaac
     3961 accgtccgcg ccgtcgtctg gcaggcggtc ggcggcaagt gggtgagggc gggggacgcg
     4021 tcccatgcat tcgtcgcagc cggtccggac cccgcaaaag ggtctgtctc cgtcagtggg
     4081 ccgtcgtccg ttgaagtggg agacaggatc gatctcaatg ccagcgtcgg tgacacgaac
     4141 gtgccggcat cggcgctcta ctacagctgg gtggtcaacg gcagaagata caactacagg
     4201 gaatccatcg ccctgccggc aagcgctccc ggcacgtata acgtgaccgc ggagctgtgg
     4261 atgaagtacc agccgaaacc ggtcaagctg gcccaggcct tccacaccgt aacgatagtg
     4321 aagaaggggg agaagcccga ccccctcggt gatctaatgg cggcgatcct tggcggaagc
     4381 gacaaacccg gcacatcgac cggcgcctcc ccgacgaagc cggacacgaa ggtccccgtg
     4441 ggcagggcgt acacggcggc gaagcttccc gcaaatcagt tttcgctcag cgggacgtgg
     4501 aacgccgtcg attccaaggg caggttcaat ggaaagatga gcctgagcca gtccggctcc
     4561 agcgtcagcg gcaccatgca gacacccggg ggcgggatcc cggtttccgg ctcgatatcg
     4621 ggcagcaccg tttccgtcat tctcacattc ggcagcccgg cgatcataaa ccagtacctc
     4681 caggacatga gagtatcgca ggcggtgggc tccatcacgg ccaaggccac cttcaccatc
     4741 ggctcgaaca acaatgagct tcagggtacc ctgtaccctt tccatgttca atggaacgac
     4801 gacggcaaga cggtgatcgt gaagcgaaag tggcagggag gcgaaacgca cccggggaat
     4861 cccccgcgct ccttcactct gagacgatag cgtaccggga gagagccgca cctcctggaa
     4921 gtcccatgcc ggggaccggt ggttcgttcc gccggcttcc acggctcccg gtctggcgcc
     4981 ggccgggaat ctgtcgttct cgatgctata tatcctgtat gaacaataag aggacatcgg
     5041 ggcttgtccc gtgaacccga cggacctact cgcgcagtca gcgaagcttg tgatacaggt
     5101 cgaggaacag gcggtcgccg ttgtcaccgc catcgccttc ttcatgggga gcatgcggac
     5161 gatcgtcacc ctttacagga aaggctacga gaacattatc ccctatctca aggaccagac
     5221 cggcagattc atgatcatct gcgcgctctg cacacccctt ccattcctgg gcggaaccga
     5281 cggcaaggcc ggtacattca tcggaacatt ccctcggatg gtcatatctg ccggattcgc
     5341 gcaggcgggc aacgagaagg tgtttgacaa cctcaaggcc gtgaaggcga agttgggaaa
     5401 ggagatgggt tccggggagc tttcggaaaa gctgacaaat atcaaggagg cctctcccct
     5461 tactggtgtg aagatcaagt gggtggatgc cataaaggtg cggctggcac agctcatctt
     5521 tcttctgccc ctcatccctc cctcatcctg tcttgccctt ttccatcctg ccctgggcgc
     5581 gttcatgatg atgataagct atgtggcatc ggaatacatg gcagtcatca cctgcggcat
     5641 cggggtgaac gtggatacct caagcatggt gttcgttctt gagaccgtcc gtgagttggt
     5701 gctgagccac atgggggcca tcacggggtt tctctttcac actctcctcg tgttcacctt
     5761 cacgggggtc atcattgccg ccgccgtaag ggctgccgta ttctgcgtca cctttccgta
     5821 ttccatcatc accatggcct tcgactcccg gcgtgaagtg ttctttcagg gcatcgtcag
     5881 ggccttcgcc atcgcgttga cccccgtcgt ggcggtcaac gtcctcaacg tcctcgccat
     5941 ggcctacgat cttttcatga gcgggggcct cgcgggcggg atgatcgagg cctatctgtt
     6001 cagcaacatc gattcgctcg tggattccgg tgtttttccg gtcgcgggag agatgtacgg
     6061 gtttgtgttc cggcttttca tcgcgaccgt cctggcgcct tcggtgttga gcatcccggc
     6121 catcgtgttc ctcatcggga gctaccgcgt cgcctccgag gccataggtg cgggattgag
     6181 ctattccggc ggtatgggca ggggcggcgg agggggtgag tgaggacgcg gagattcgca
     6241 tctcttcgat tgcgggcgac catggacctc gtctatttcg ctgtgtcccg aaggggtttc
     6301 agaaagagga gcaatggggt taaaatgaag gcgtagttgc tccatccggg gtggtagaag
     6361 gatagggcca tggccagg
//
LOCUS       DAOQTR010000046         6369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13454_length_6369_cov_4.064777, whole genome shotgun sequence.
ACCESSION   DAOQTR010000046 DAOQTR010000000
VERSION     DAOQTR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13454_length_6369_cov_4.064777"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKN85_01940"
     CDS             <1..904
                     /locus_tag="PKN85_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT43190.1"
                     /translation="LSWRWMFFINIPIGCVAILQVIRAIPNDPVTGRRRSRIDYAGAV
                     LSMLSLLSLLFTLNLGSELGWLSLPIVLFFLGFAVFAFLFIVVEKRAADPLVHLDIFS
                     DRSFLLTLCATVTALMILSGTGILMPFYLEQIKGMTADKVGLFMLIYTGIFAVVSSGF
                     AGKLSDRITPAKICAAAIFSVALSALFFAFTLALPGYTFVIISFVWMGASLGFFMPSN
                     NNQVMNKATPETKGEVSGLLNTAVNLSFTLGICVFETVYSLDTDILRGFRNAYVFGFI
                     ACFAAMVLSLLSIRVKGRTGGERG"
     gene            929..1117
                     /locus_tag="PKN85_01945"
     CDS             929..1117
                     /locus_tag="PKN85_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43191.1"
                     /translation="MGHIGPTELRLDRLPGESFLYGQLALATGGGWSVPSRGKIVVPL
                     VGPKRIAGKEQFREVMVR"
     gene            1114..1872
                     /locus_tag="PKN85_01950"
     CDS             1114..1872
                     /locus_tag="PKN85_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43192.1"
                     /translation="MRGILRDDERARVDEGVKEIERRTGAQIVVAVVERSDSYVELPW
                     KAFALGASITCLVIFALGFFAPYRASQATGFVAIAGMLAFGGLCALLTVLMPGFARLF
                     LSRYRSETEARQYAESLFLKREIFATAGRTGILLFVSLFEGQAVLLPDRGLAGSLAGE
                     PSSPSSRQWRHRSPAAGQARPSRRVSAGSSTSSGGQNWPVPGMRERTNSPTRSSRRGV
                     HEKNILSGLPFGDPPRSFDGHGRRRALSHGPGDR"
     gene            1769..2707
                     /locus_tag="PKN85_01955"
     CDS             1769..2707
                     /locus_tag="PKN85_01955"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNT43193.1"
                     /translation="MKRTFFPAFLSVILLVLSTAMAADVPYLTGRVTDNAGILSAESR
                     KALTEKLKVYEDRTGNQVVVLTIPTLDGEGIEEYAVKVFESWKLGQKGKDNGVLMIVV
                     PEDRRMRIEVGYGLEGTLTDLMAGRIIQQVMTPRFRNGDYDGGITEGAAAVIKVLEGG
                     EAPPATGGTGSSVRSGGITEPDLPIAQRILFGAFIFGIIGLFTVIGIVTPGVGWFLYF
                     FLIPFWAMFPIVVLGVRGALICLAGYLVVFPVAKLLLKSSDFYRKGVMSLRAKGGASI
                     GGFTFSSGRSGGFSSSSSGFSGGGGSSGGGGSSGSW"
     gene            2847..4037
                     /locus_tag="PKN85_01960"
     CDS             2847..4037
                     /locus_tag="PKN85_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNT43194.1"
                     /translation="MKRSLLAFLGSLLLAVLSMTPAAASPAGPFSAISDGNAGLAVQL
                     YRELGAQEGNLFFSPYSISSAMGMTYAGARGETAAQMKTTLHFRLEQASLNSGFRSLN
                     KILYENARSSGQRLSIANALVLTGDSVSDAYRNMLTTYYDAAIFRGGLEEINGWVKTK
                     TEGKIDRILEQLSANSVCVILNAIYFKGVWESQFDKKLTFDGPFRLPGGIDVKTSFMH
                     RKAEFKLLEEKDFQVLSLPYKGGTLSMVVLLPKTVDGLARLEDALYAEDLKGWLARLD
                     KQRNQKVSVYLPKFKLETKYDLVAPFQKMGMKDAFSPAADFSGTGWRKGDLWIGQIKH
                     RAFVEVNEEGTEAAAATAVEMVTKSAPPRELVFRADHPFMFLIRDNETGTILFIGRMA
                     NPAK"
     gene            4132..4501
                     /locus_tag="PKN85_01965"
                     /pseudo
     CDS             4132..4501
                     /locus_tag="PKN85_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013119.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
     gene            4697..5233
                     /locus_tag="PKN85_01970"
     CDS             4697..5233
                     /locus_tag="PKN85_01970"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNT43195.1"
                     /translation="MIVSRTRILSMLALVLGLTLLVCFTVPAQAAPQAGPKAGPIEPY
                     KDTARVMTHSFAAALGGALTNVKNEKDRVDLIRSFIAPVRFYADKSGYFYVYDSKCVN
                     VAHAIQKDLQGKNLYDHKDAKGKYVIRALLEAAKKGGGFVDFYWVKPGSKTEAAKLGY
                     VEPIPGTDYFIGTGVYLP"
     gene            5680..6156
                     /locus_tag="PKN85_01975"
     CDS             5680..6156
                     /locus_tag="PKN85_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT43196.1"
                     /translation="MDERNVLIAFDGSESAFKAVDYVADQFSGLKDIRVTLLYVIPNV
                     PPEFWDDGHILTPEEKRDRQVVIDRWFANQKMVLEPHFEKARRVLVEKGMEAGQVETV
                     VAGDTVTVAEAILEEAKTGRYRTLVIGRHGAVPILAGGLAATLLHRGAGLAVCIVE"
BASE COUNT         1282 a         1834 c         1976 g         1277 t
ORIGIN      
        1 cctttcctgg cggtggatgt tcttcatcaa catccccatc ggctgcgttg ctatcctgca
       61 ggtgataagg gcgataccca acgaccccgt caccgggcgg aggcgctcga ggatcgacta
      121 cgcgggggcg gtcctcagca tgctctcgct cctcagcctt cttttcacct tgaacctcgg
      181 cagcgagctt ggctggttgt cgctgcccat cgtccttttc ttcctggggt tcgccgtctt
      241 tgcctttctc ttcatcgttg tggagaagcg cgccgcggat cccctcgtcc acctcgacat
      301 cttttccgac cgcagtttcc tgctcaccct ctgcgcgacg gtgacggcgc tcatgatact
      361 gagcggaacg ggtatcctca tgccctttta cctcgaacaa ataaagggca tgacggcgga
      421 caaggtcggc ctcttcatgc tcatctacac cggcatcttc gccgtcgtct cctccgggtt
      481 cgccggcaag ctctcggaca ggataacgcc cgcgaagata tgcgccgcgg ccatcttcag
      541 cgtggccctc tcggctctct tcttcgcatt tactctcgcg ctaccgggct acaccttcgt
      601 catcatctcc ttcgtgtgga tgggcgcgtc cttaggtttc ttcatgcctt ccaacaacaa
      661 ccaggtcatg aacaaggcga cgcccgagac aaagggagag gtttcaggtc tcctcaatac
      721 cgcggtcaac ctgagcttta ccctggggat atgcgtcttc gagaccgtct attccctgga
      781 caccgacatc ctccggggct tcaggaatgc ctatgtgttc ggattcatcg cctgcttcgc
      841 cgcgatggtg ctctcgcttc tgtcgataag ggtgaaagga aggacaggtg gggagagggg
      901 ataggaccta taggagataa tgggacctat gggacatata ggccccacgg aactgcgtct
      961 ggacaggttg ccaggagaga gctttctcta cggtcaatta gcgcttgcaa caggtggtgg
     1021 gtggagtgta ccctctaggg ggaagatcgt ggtgcctttg gtggggccaa agaggattgc
     1081 cgggaaagaa cagtttcgtg aggtgatggt gagatgaggg gtatcctgag ggatgacgag
     1141 agggcccggg tcgatgaggg cgtaaaggag atcgagaggc ggacgggggc gcagatcgtg
     1201 gttgccgtcg tggagcgcag cgacagctac gtggagctgc cgtggaaggc cttcgccctt
     1261 ggcgcgtcca tcacgtgcct cgtcatattt gccctggggt tcttcgcgcc ctaccgggcc
     1321 tcgcaggcga cggggtttgt cgccattgcg ggaatgcttg ccttcggagg tctctgcgca
     1381 ctccttaccg ttctcatgcc gggcttcgcg cggctctttc tgtcgcggta ccggtcagag
     1441 acggaggcgc ggcagtacgc cgaatcgctc ttcctcaagc gcgagatctt cgcgaccgcg
     1501 gggaggacgg gcatcctcct gttcgtgagc ctcttcgaag gacaggcggt ccttcttccc
     1561 gaccgcggcc ttgcggggtc cctggcgggg gagccatcga gtccgtcgtc gcgccaatgg
     1621 cggcatcgct ctcccgcggc aggacaggcg aggccctcga gaagggtctc ggcaggctcc
     1681 tcgacatcct cgggggggca gaattggccg gtcccgggga tgcgggagag aacgaactcc
     1741 ccgacgaggt catcgaggag aggcgtgcat gaaaagaaca ttctttccgg ccttcctttc
     1801 ggtgatcctc ctcgttcttt cgacggccat ggccgccgac gtgccctatc tcacgggccg
     1861 ggtgaccgat aacgccggga tcctctccgc agagagccgc aaggcgctta cggaaaagct
     1921 caaggtctac gaggaccgca ccgggaacca ggtagtcgtc ctcaccatcc ccacccttga
     1981 cggcgaaggt atcgaagaat acgccgtcaa ggtcttcgaa tcatggaagc tgggccaaaa
     2041 gggaaaggac aacggggtcc tgatgatagt cgtcccggag gacaggcgga tgcgcatcga
     2101 ggtgggctat gggctcgaag gcacactgac ggacctcatg gcgggcagga tcatccagca
     2161 ggtcatgaca ccccgcttcc gcaacgggga ttatgacggc gggatcaccg agggtgccgc
     2221 ggcggtcatc aaggtgctcg aaggcggcga ggccccgccg gcaacgggcg gaaccgggtc
     2281 ctccgtgagg tccgggggca tcacggagcc cgaccttccc atcgcccagc gcatcctctt
     2341 cggcgctttt atcttcggta tcatcgggct cttcaccgtc atcggcatag tcacgcccgg
     2401 agtgggctgg ttcctgtact tctttctgat acccttctgg gccatgttcc ccatcgtcgt
     2461 tctcggcgtc aggggagcgc tcatctgcct tgccggatac ctcgtcgtct tccccgtggc
     2521 gaagcttctt ctcaagagct ccgactttta caggaagggt gtgatgagcc ttcgagccaa
     2581 gggcggcgcc tccatcggcg gtttcacctt cagctccggc cgcagcggcg gtttctcgag
     2641 cagcagctcg ggtttctccg gcggcggcgg atcctccggc ggcggcggct cgtcgggaag
     2701 ctggtaagcc ggtaagccgg gaaacgttct gcggacaacg gcgaaaaagg gaccggcagg
     2761 aacgggggaa ctttttcaga gaccctcgtg tccaacggga gagaaagcga agacgcgatg
     2821 atatccacag ccgaaaggag aatgaaatga aacggtcgtt attggcgttc cttggcagcc
     2881 tcttgcttgc cgtcctgtcg atgacccctg ccgcggcatc acctgcgggt cccttcagtg
     2941 ccatttccga tggaaatgcc ggattggcgg tgcagttgta ccgtgaactg ggggcacaag
     3001 aaggcaacct ctttttctca ccctacagca tttcgtcggc catgggcatg acctatgccg
     3061 gggcacgggg ggagacggcg gcgcagatga agaccaccct ccatttccgc ctggagcagg
     3121 ccagtttgaa ctccggcttc aggagcctca acaagatcct atacgagaac gccaggagtt
     3181 cgggtcagag gctcagcatt gccaacgccc tcgtcctgac gggagatagc gtgagcgatg
     3241 cctaccgcaa catgctgacg acctactacg acgccgcgat cttcagggga ggcctcgagg
     3301 agataaacgg gtgggtcaag acgaagacgg aagggaagat cgacaggata ctcgagcaac
     3361 tctcggcgaa ttccgtctgc gtcatcctca acgctatcta tttcaagggc gtctgggaaa
     3421 gccagttcga caagaagctc accttcgacg gccccttcag gctcccgggc ggaatagatg
     3481 tgaagacgtc cttcatgcac cgcaaggccg agttcaaact gctcgaggag aaggacttcc
     3541 aggtcctgtc cctgccctac aaggggggaa ccctctccat ggtggtcctc cttccgaaga
     3601 ccgtcgacgg cctcgccagg ttggaggacg ccctctacgc ggaagacctc aagggatggc
     3661 tggcgaggct tgacaagcag aggaaccaga aggtatccgt ctaccttccg aagttcaagc
     3721 tcgagacgaa gtacgatctc gtcgcgccct ttcagaagat gggcatgaag gacgccttct
     3781 ccccggcggc ggatttctcc ggcacggggt ggaggaaggg agacctctgg ataggccaga
     3841 tcaagcacag ggcttttgtg gaggtcaacg aggagggaac ggaggcggca gcggccacgg
     3901 ccgtggagat ggtgacaaag tcggccccgc cgcgcgagct cgtcttccgg gcggaccacc
     3961 ccttcatgtt cctcatccgg gacaacgaga cggggacgat tctgtttata gggaggatgg
     4021 cgaacccggc gaagtgaaag acggttcaag agttcaaggg ttcaaaagtt caagagtagg
     4081 aaagaccgcc gcctgacccc ggagaaggtg cgttccctca gcctccccat gatggtcgag
     4141 cgtaactcca gccgtttcca gacagccttc accgtgtcca tagaggccgc ccggggtgta
     4201 acgacgggcg tattcgccgc cgacggtcga cctcgcgcgg atggcggggt ttaagccctg
     4261 ctgtgtgctc tgcgagctta cgaaccccga cgggaccatg gcccgcctgc cggacatcgt
     4321 gaggtttgcc gagaggcact ccatgcccgt cgtcaccgtc gcggacctgg tggagtaccg
     4381 gtgtgcgaga gaactgatgg cgagctggag gccggcacat atcgataagg gagcgacgac
     4441 catggaaaaa gaatgccata acatctacga agagatgtcc atgtggatgc ttgaaccttg
     4501 aggcttgttc tcctttctgt taagcttcaa gacgcccggc gcgataacac aggcatatac
     4561 ggttcgtttt caactagccg tgaaccggaa ggatacggca attggccgtt ctattgtttc
     4621 agggctgcag gataacaaga cggtattcga acccttgagc cctggaacca tcttaatgac
     4681 aggaggattt accgttatga tcgtatcacg tacccgcatt ctctcaatgt tggcactcgt
     4741 tctcggcctc acgttgctcg tgtgtttcac ggtccccgcg caggcggccc cgcaggcagg
     4801 gccgaaggcc gggcccatcg aaccgtacaa ggatacggcc agggtcatga cccacagttt
     4861 tgccgccgcc ctcggtggag ccctgacaaa cgtgaagaat gaaaaggacc gcgtcgacct
     4921 tatccggtcc ttcattgccc cggttcgctt ctatgcggat aaatcggggt acttctatgt
     4981 ttacgattcg aaatgcgtca acgtcgccca tgccatccag aaagacctgc agggcaagaa
     5041 cctgtacgat cacaaggacg cgaagggcaa atacgtcatc cgtgcattgc tggaagcggc
     5101 aaaaaaaggc ggcggcttcg tcgatttcta ctgggtaaaa ccgggctcca agactgaggc
     5161 ggccaaactg ggttacgttg aacccatccc cggcacagac tacttcatcg gcacgggcgt
     5221 atatctgccc tagaaaagag actttatctc acgcccgctt cgctcgaggg ctcagaggtc
     5281 acgaagagag gagaagaaag agaaaagact gccccgtgat cccggctccg gccgggatca
     5341 cggggccttc tctcccccca cactgtctcc ccccacaact cctatccgcc ccacccacca
     5401 tctccacctg aaaagaaaac cctctttttt tgtcccgcga agcaggatgc aaggacgctt
     5461 tgatgccggc gggcgccggg gtcaaagcga atcttttctg tgtgctctcc tcaaaacctc
     5521 tgcgccctcg agcgaagcgg gcgtgagaca gatccttcgg ccttccgcgg ctggcggact
     5581 gcacatgcca gcgacagggt tgggcggatc cctttcgtgt tgtgcatccg ggataaggtg
     5641 ctaggcttta tagtagagcg tgcagcagga ggaggggtca tggatgagag gaatgttttg
     5701 atcgcctttg acggttccga aagtgccttc aaggctgtag actatgtcgc agaccagttc
     5761 tcggggctaa aggatataag ggtgacgcta ctttatgtga ttcccaacgt acctccggaa
     5821 ttctgggatg acggccacat cctgacaccg gaggagaagc gggaccggca ggtggtcatc
     5881 gacaggtggt tcgcgaacca gaagatggtc ctcgagcccc acttcgaaaa ggcgcgccgg
     5941 gtgctggtgg agaagggcat ggaggccggg caggtcgaga cggtggtggc cggcgatacg
     6001 gtcaccgtgg cggaggcgat cctggaagag gcgaagacag gtcggtaccg caccctcgtg
     6061 atcggcagac acggcgccgt ccccattctt gccgggggtc tggcggcgac gctcctccac
     6121 aggggagcgg ggctggcggt gtgcatcgtg gaataggcga gacgctgatc ttcaagcctt
     6181 ttcatagtgg acgaaaaaat tgaggtctcc cgatggctgg ttggggtaga atggtagggt
     6241 tcgaaagaga tatgaggagg gacctgatgg ataaggtgcg gataaacagc gagacgatcc
     6301 tttaccccat gccctgttcc atcgtcggca cgctggtgga aaagaagccg aactatctcg
     6361 ccgttgcat
//
LOCUS       DAOQTR010000047         6323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13591_length_6323_cov_3.485960, whole genome shotgun sequence.
ACCESSION   DAOQTR010000047 DAOQTR010000000
VERSION     DAOQTR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13591_length_6323_cov_3.485960"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="PKN85_01980"
     CDS             <1..1984
                     /locus_tag="PKN85_01980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT43197.1"
                     /translation="DINPLLLYPTGCVAVDARVFVDHSPRISPQAAPRAKDDLRRLFY
                     PKSIAVLGATDSGGKLGYNVMRNLLSHQFEGKLYPINPRKGTVLGVRAYPSILDVKDP
                     VDTAIIIVPAGAAPQAIEDCCRKGVRNLVVETAGFAETGEAGRRTQARIKDLVTAKGC
                     RLLGPNCSGIINTHHNMVQSIGLVGELKRGNVGLIAQAGVYAAGMLTGLHNVLDFGMI
                     ATIGNKMDVSEADILEFMGEDPNIRVVGMYMEDVTSGRRLVDVAGRVSTKKPVIALKT
                     GRTEAGKKAVSSHTASMAGNDEINSAAFRQSGIIRARDNEHLFSLMRAFSKQPLPKGP
                     GVLVVTYTGSLGVAATDMLYIRGLTLAELEPCFKERLAPYLDDYLNVQNPVDCSFNMD
                     ATQVRQLISIGVESPEVNAIIVIVQGEILGSYVETLASIDYRGKPVLCCVACKEFMMD
                     HVVEMEQKGIPVYSTPEMAVDVLSDMYAHRRRQSAARLADLNGFLADRSFAIAGRQVH
                     LRLLTQNDIDLWTDFVKSCSPRSLWMRFLSPFDPTPEAARRYCVIDPEEEVAVVAEAV
                     EADRRKLVAIARLIKCGPTDQAEYAVIVTDSWQKRTLGRLLLEACLDLSKHLGIRVVN
                     AEIVQENFPMNKVLNHCNFTMHAKERNMLLMSRNLE"
     gene            complement(2319..4292)
                     /gene="acs"
                     /locus_tag="PKN85_01985"
     CDS             complement(2319..4292)
                     /gene="acs"
                     /locus_tag="PKN85_01985"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNT43198.1"
                     /translation="MANVNETDFYDKVFPVPDRVREKSYIKSRAEYDKLYKESIENPD
                     AFWAKEAEKRLTWFKPFDKNKVSDWSFGDDLHVKWFEGGKLNVSYNCLDRHLETKKNK
                     AAIIFEGNDPKDWKVFTYWDLYREVNKFANVLKGLGLKKGDVASIYLPMIPELVISML
                     ACARIGVVHAIVFGGFSAEALRDRINDCKSTVLITCDGTYRGAKAVPQKDNADKAADQ
                     CPSIKTEIVVKRTGTPVTMKDGRDVWWDDLMAKADSYCKPEEMDAEDALFILYTSGST
                     GAPKGVQHSTAGYLLYASMTQQLAFDVRDEDVYWCTADIGWVTGHSYVAYGPLTNGHT
                     SILFEGVPSYPGFDRFWEVVEKYNVNNFYTAPTAIRAVAKEGDEWVEKHDLSSLRVLG
                     SVGEPLNPEAWWWFYNKVGGGRCTIVDTWWQTENGGHMILPLPGAVDIKPAKCMQPFF
                     GVIPAIMDEDGKEIVGPGKGALCMKTAWPGMMRGVWGNPKLFKENYFSQFPGHYFSGD
                     GAERDADGDYKITGRIDDVINVSGHRLGTAEIEAALTSHPKVAESAVVGFPHDIKGQG
                     IYAYVTLNTGVEKDDALKKDLIALVRKEIGPIATPDLIQWADGLPKTRSGKIMRRVLR
                     QVAAEKFEDFGDTSTLAEPHVVDDLVAERKKIK"
     gene            complement(4527..5333)
                     /locus_tag="PKN85_01990"
     CDS             complement(4527..5333)
                     /locus_tag="PKN85_01990"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT43199.1"
                     /translation="MKGARKTAALISMLLVAAAIAGCDVLQDHFIYFPDHIRPSAGAL
                     TARGLTVWMASGDDYRGLVSAPESDAPAGTVIVFHGNAGKAADRGFYAKMLVPLNYRV
                     ILAEYPGYGGRPGSRGEDQFVKDARLTVEAAFAKYGAPLYLLGESLGCGVVAAVAGGS
                     PVPVEGIVLFTPWDDLLSVAASHAPAFIARIVLKDRYDSVANLANYAGKTAIIGAERD
                     DIIPVVHAEALYASIPGRKRMWTIKGAGHNDWPMLASGAMFREIMDFVSR"
     gene            5663..6220
                     /locus_tag="PKN85_01995"
     CDS             5663..6220
                     /locus_tag="PKN85_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43200.1"
                     /translation="MTMTAAVRSILVLQLIVLSLALCVPAFADTPAPPRDYAKETENG
                     QYIFVMLAPPERWVSKDTELRKTYKASGLYRNDGSATPLWTVYWYSFSVYPSSDGRHI
                     VRMGPWASSVDQLALAFYENGKELKSYLIRDLIKNQLKLKHTVSHFFWQKELKYNDKE
                     GTILIKTHDDQTYLFSVKTGEILQR"
BASE COUNT         1312 a         1913 c         1816 g         1282 t
ORIGIN      
        1 cgacattaac ccccttcttc tctaccccac cggatgcgtc gccgtggatg cccgcgtatt
       61 tgtcgatcat tccccccgga tctctcctca agcggctccc agggcaaagg acgatcttcg
      121 cagactgttc tatcccaaga gcatcgctgt gctgggagcc accgattccg ggggcaagct
      181 gggatataac gtcatgcgaa acctgctttc ccaccagttc gaaggaaagc tctatcccat
      241 aaatccccgg aagggaacgg tgctgggggt cagggcctac ccgtcgatcc tcgacgtgaa
      301 agaccccgtg gacacggcga tcatcatcgt acccgccggg gcggccccgc aagccatcga
      361 agactgctgc aggaaggggg tcaggaatct cgtggtggaa acggcaggct tcgcggagac
      421 gggagaagcc ggcagaagaa cacaggcccg catcaaggac cttgtcacgg caaagggatg
      481 ccgcctgctc ggccccaact gttcgggcat catcaacacg catcacaaca tggtccaatc
      541 gatcggactc gtcggcgagc tcaagagggg gaatgtgggg ctcatcgccc aggccggggt
      601 ctacgcggca ggaatgctca cggggctgca caacgtactc gatttcggga tgatcgccac
      661 catcggcaac aagatggatg tcagcgaggc cgatatcctc gaattcatgg gagaggatcc
      721 gaacatacgt gtggtgggga tgtacatgga ggatgtgacg agcggccggc gtctggtcga
      781 tgtagcgggg cgtgtctcaa cgaagaaacc cgttatcgcc ctcaagacag gccggaccga
      841 ggcgggaaag aaggccgtct cgtcacacac cgcctccatg gccgggaacg acgagatcaa
      901 ctccgccgca ttccggcaaa gcggcatcat ccgggcccgg gacaacgagc acctcttctc
      961 cctcatgcgg gccttttcca agcagcccct gccaaagggg cccggcgttt tggtcgtgac
     1021 atacacgggg tccctcggtg tcgcggccac ggacatgctc tacatcaggg ggctcacgct
     1081 cgcagagctc gagccgtgct ttaaggagcg gctggcgccc tatctcgacg attacctcaa
     1141 cgttcagaac cccgtggact gctcctttaa tatggacgcc acacaggtac gccaactcat
     1201 cagcatcggc gtcgagagtc ccgaggtcaa cgccatcata gtcatcgtcc agggcgagat
     1261 actggggtcc tacgtggaaa cgctggcctc catcgactac agggggaaac cggtcctgtg
     1321 ctgcgtggca tgcaaggagt tcatgatgga ccatgtcgtc gaaatggagc agaaaggcat
     1381 tcccgtgtat tccactcccg aaatggccgt cgacgtcctg tcggacatgt acgctcaccg
     1441 ccgcaggcaa agcgccgccc gcctggccga cctcaacggc ttcctggcgg accgctcctt
     1501 cgcgatagcc ggccggcagg tacacctgag actgctcacg cagaacgata tcgacctctg
     1561 gacggatttc gtgaagagct gctctccacg gtctctctgg atgcgcttcc tctccccctt
     1621 cgaccctaca ccggaagccg cccgccgtta ctgtgtcatt gaccccgagg aagaggtcgc
     1681 cgtggtcgcc gaggccgtgg aggcggaccg cagaaagctc gtggccatag cgcggcttat
     1741 caagtgcggt ccaacggacc aggcggaata tgccgtcatc gtcaccgact cctggcagaa
     1801 gagaacactg ggccgcctgc tcctcgaagc gtgcctcgac ctttcgaagc atctcggcat
     1861 ccgcgtcgtc aacgccgaga tagtgcagga gaacttcccc atgaacaagg tcctcaacca
     1921 ctgcaacttc acaatgcacg ccaaggaacg caacatgctc ctcatgtcga gaaacctcga
     1981 gtagaaaacc gcttgaaccg cttgaaccgc ttgaatgctg cgcaggccgt gaggttggct
     2041 cttgcatggc gccgcctgcc cgaatatccg caacatgccg tcattcttct tttgacgctc
     2101 aagcggttca agcggtcttc tttctttact tttcttctga gttttagtat agaatagtga
     2161 gttgagggag atgtgggttg gaaataggat ccccctcaac tctggcagac gccgatcgta
     2221 atacgggtac gatcgacaga agtctccttg aaacacctga aatattcagg agaaggaccc
     2281 ggctactgac gtgaagtctg gccgggtcct tttttacctt actttatctt ctttctctcc
     2341 gctaccaggt catcgacaac gtgaggctcg gccagcgttg aggtgtcgcc gaagtcctcg
     2401 aacttctcag ccgcgacctg cctgagcacg cggcgcatga tcttgccgga ccttgtcttg
     2461 ggaagcccgt ccgcccactg gatgaggtca ggagtggcga tggggccgat ctccttgcgc
     2521 accagggcga tgagatcctt cttcagggca tcgtctttct cgacgcccgt gttcagggtg
     2581 acgtaggcat agataccctg acctttgatg tcatgcggga agcccacgac cgcggattcc
     2641 gcgaccttcg gatgggaggt caatgcggcc tcgatctcgg cggttcccag cctgtggccg
     2701 gagacgttga tgacgtcgtc gatccttccg gtgatcttgt agtcgccatc ggcatcgcgc
     2761 tcggcaccgt cgccggagaa gtagtggccg gggaactggg agaagtagtt ttccttaaag
     2821 agtttcgggt tgccccacac gcccctcatc atgccgggcc atgcggtctt catgcagagt
     2881 gcgcccttgc ccgggcccac gatctctttg ccgtcctcgt ccataatggc cgggataacg
     2941 ccaaagaaag gctgcataca tttcgccggt ttgatgtcga ccgcgccggg gagaggaagg
     3001 atcatgtgtc cgccgttctc cgtctgccac cacgtatcga cgatggtgca cctgccgccg
     3061 ccaaccttgt tgtagaacca ccaccaggcc tcggggttca gcggctcgcc gacggaaccg
     3121 agaacgcgca gggaggagag gtcgtgtttc tcgacccact cgtcgccttc cttggcaacg
     3181 gcgcggatgg ccgtcggggc ggtgtagaag ttgttgacgt tgtatttttc caccacttcc
     3241 cagaaccggt cgaaaccggg atagctggga acgccctcaa agaggatgct ggtatgtccg
     3301 ttcgtcagag gcccgtaggc gacatagctg tgacccgtga cccagccgat atcggccgtg
     3361 caccagtaaa cgtcttcgtc acgcacgtcg aaagcaagct gctgcgtcat ggatgcgtaa
     3421 agcagataac cggcggtcga atgttgaaca cccttgggtg ctcccgttga gcctgacgta
     3481 tagaggatga agagcgcgtc ttcggcgtcc atttcctcgg gtttgcagta gctgtcggcc
     3541 tttgccatca ggtcatccca ccacacgtcc cggccgtcct tcatggtgac gggagtgccg
     3601 gtacgcttca cgacgatctc ggtcttgatg gacgggcact ggtctgccgc cttgtcggcg
     3661 ttgtccttct gcgggactgc cttggcgccg cggtaggtgc cgtcgcaggt gatgagcacc
     3721 gtggatttgc agtcattgat cctgtctctc aaggcctccg ccgagaaacc gccgaagacg
     3781 atggcgtgca ccacgcctat cctggcgcag gcaagcatgg agatgacgag ttccggaatc
     3841 atcgggagat agatcgaagc gacatcgccc ttcttgagtc cgaggccctt caggacgttg
     3901 gcgaacttgt tcacttcccg gtacagatcc cagtaggtga aaaccttcca gtctttgggg
     3961 tcgttgcctt caaagatgat ggccgctttg ttctttttcg tttccaggtg ccggtcgagg
     4021 cagttgtagc tgacgttgag cttgccgccc tcaaaccatt tcacgtggag gtcgtcgccg
     4081 aaagaccaat cggagacctt gttcttgtcg aagggtttga accaggtgag ccttttttcg
     4141 gcctctttcg cccagaaagc gtcagggttt tcgatggact ccttgtagag cttatcgtac
     4201 tccgccctgc tcttgatgta cgatttttcc cttaccctgt caggaacggg aaacaccttg
     4261 tcataaaaat cggtttcgtt gacgtttgcc attcttcttc tccttgtcct tcaagatttg
     4321 ccgatacggc actcacagcc gcattctctc atcaattacc aatcccacgg tatcaggtta
     4381 gaataaatca tgagtgtttg gtatcgggat ggggatgaat tagatgtggg ttaaacgcct
     4441 acaaaccata gtcgccggac cgacgctcct gcacagaaaa gaccggccgc ggcgcgggcg
     4501 tcgaggaagg caaaggtccc ccggaactat ctcgagacga aatccattat ctccctgaac
     4561 atcgcgccgc tcgcgagcat gggccaatcg ttgtgtccgg cgccctttat ggtccacatg
     4621 cgctttctcc cgggaattga cgcgtagagc gcctcagcat ggaccacggg aatgatgtcg
     4681 tcgcgctcgg ctccgatgat cgccgtctta ccggcataat ttgccaggtt cgccacgctg
     4741 tcgtaccggt ccttcaggac aatccgggca atgaaagcgg gggcatgcga ggccgccacg
     4801 gacaggaggt catcccaggg cgtgaaaagc acgatcccct ccacgggcac gggcgagccg
     4861 ccggccacgg cggccacgac accgcacccg agggattctc ccaaaaggta caggggcgcg
     4921 ccgtactttg cgaaggcagc ctcgacggta aggcgggcat cctttacgaa ttggtcttct
     4981 ccccggcttc ccggccgccc tccatacccg ggatactcgg caaggatgac acggtagttg
     5041 agagggacga gcatcttcgc gtagaacccg cggtcggccg ccttgcccgc attgccatga
     5101 aagacaatga ccgtgcctgc aggggcgtct gactccggcg cgctgacaag cccccggtaa
     5161 tcatcccccg acgccatcca gacggttagg ccccgcgccg tgagggcccc ggcggagggc
     5221 cggatgtgat cgggaaagta tatgaaatgg tcctgcagca catcacaccc cgcgatcgcc
     5281 gctgccacga gcagcatcga tataagcgcc gctgtcttcc tggctccctt catgcgtccg
     5341 ttgaccacca ccctttaaga caattataaa ggaagacgac ccgcagggga ataccgcggg
     5401 ccaaacctga cttttcctgc gcaccctccg gggagccggc catgccccgc atcgtgtccg
     5461 ctgtggcaac gagctgcttg ccgaccactg ccggtcgctg ggggttcccg tcatatggct
     5521 gcggcagacc gagcgagtgt aggagttcaa gaagcacaaa ggcagggacg acggacaggc
     5581 aaaagagaca acgatcccac gcttgtattg accccccggg cgtagtactc tatagaccgg
     5641 ttcctacact atccgggagg aaatgaccat gactgccgcc gtgagatcca tccttgtact
     5701 tcagctgatc gtcctgtccc tggcgctgtg cgttcccgcc ttcgccgata cgcccgcccc
     5761 tccccgcgat tacgcgaagg agacagagaa cgggcagtac atcttcgtca tgctcgctcc
     5821 gcccgaaaga tgggtgagta aggacacgga gctcagaaag acctacaagg catcggggct
     5881 ttacaggaac gatggttccg ccacccctct gtggaccgtc tactggtact ccttctccgt
     5941 ctatcccagc tcggacggcc ggcacatcgt ccgcatgggg ccctgggcat cgtcggtcga
     6001 ccagctggcg ctcgccttct acgagaacgg aaaggagctg aaaagctacc ttatcaggga
     6061 cctgatcaag aaccagttga aactaaagca caccgtgagc catttcttct ggcaaaagga
     6121 actgaagtac aacgacaaag agggtaccat cctcatcaag actcacgacg accagaccta
     6181 cctcttctcg gtgaagacgg gggaaatcct ccagaggtaa acgggcatac gggtctgcgc
     6241 gtgaggcgtg taaacgagca tacggggcaa gaaaacagaa aagaaaatag aatggcatct
     6301 tttccccgta tgcccgtttt tcg
//
LOCUS       DAOQTR010000048         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_13878_length_6234_cov_3.714031, whole genome shotgun sequence.
ACCESSION   DAOQTR010000048 DAOQTR010000000
VERSION     DAOQTR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13878_length_6234_cov_3.714031"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PKN85_02000"
     CDS             <1..654
                     /locus_tag="PKN85_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43201.1"
                     /translation="YRSAYNAANNVYHNKLQGVWTSTYFTEKRRKLDEFVRSLEADYQ
                     GLLDLLRPYRDQPWGLAMWNRVQRDYRIARKIRTDFPEERWEQHLYGRINVLLSPEGN
                     GCPQPGRGKMCSSREIDVYNGYVREVQSIGQRSYALAKSAQDHAKRALECSGRMPEPK
                     PARPSGTQRPPPSPPADSAGCRANEQKCGTWCCQRGVSCFDAYSMKMAISQGRCRTP"
     gene            810..1490
                     /locus_tag="PKN85_02005"
     CDS             810..1490
                     /locus_tag="PKN85_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43202.1"
                     /translation="MKWLLLVVFVLMSVSPVLAQTQMFNNPRVNGYAVDRCLYSGQQC
                     NQPAADEFCRRNGFQRARRFAWDFMAPTLILATGQICKVPGRGGCGGFTFIECQARGQ
                     GGGGFGHGERTFNNPRMNGYAVDRCLYYGQQCNQPAADEFCRRNGFQRARNFAWDFMA
                     PTRILATGQICNVPGRGGCGGFTRITCVGGRGGGPNPPPRGGKGPGDPCQTREECRSG
                     ICLLGVCN"
     gene            complement(1624..2409)
                     /locus_tag="PKN85_02010"
     CDS             complement(1624..2409)
                     /locus_tag="PKN85_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT43203.1"
                     /translation="MRISISSERVEELKEWFGDYVHTFQYSDPERQQSIDMKKWHTLR
                     VCAEISSLGTRLGLNEEELRLAEIIAMLHDVGRFEQYARYRTFVDGRSENHAELGVAT
                     IMGSGILDLFDDAVRDLIVRSVRYHNRASLPSDESETCLFYSRLVRDADKLDIWKVVT
                     DHYLRKDKDPNKAIELDLPDTPGFSEEVYHDVMNKRIVNMAHIKNLNDFKLLQIGWVF
                     DVNFQPTLDRVRECRYLEMIRETLPPSERINRIFGVIDRSCFR"
     gene            complement(2460..2627)
                     /locus_tag="PKN85_02015"
     CDS             complement(2460..2627)
                     /locus_tag="PKN85_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43204.1"
                     /translation="MVDDDPYRSNAKQYDEWFDCEEYKYSFAGEEVKRYLSAEEWWLQ
                     KFGNDLLWAKP"
     gene            complement(2641..3345)
                     /locus_tag="PKN85_02020"
     CDS             complement(2641..3345)
                     /locus_tag="PKN85_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNT43205.1"
                     /translation="MSAIALVGGLTTLLGPVVLERVLLPLVALASGTLLGGGFFHMIP
                     EGGKLLSPLGAALWLMGGFSAFLALEQFLHWHHSHSKPAEAKKPVTYLILIGDAVHNF
                     LGGLGIGSTFLISPKAGVIAWIAAAAHEIPQELGDFGVLVHGGWPKRRALLWNLFSAL
                     SFPLGGLIAWIASRSFNVSGLVLFGAGSFIYIAASDLIPEIKAQESLPRAILHFLFFA
                     VGLAMMFALAYCFQRN"
     gene            complement(3645..3842)
                     /locus_tag="PKN85_02025"
     CDS             complement(3645..3842)
                     /locus_tag="PKN85_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43206.1"
                     /translation="MDEIAKVNLKEFEKKPDGSWVCIQNSDITMKTGKIIRVAPGTVF
                     RKGTMFVGIDMAEALDKVPAN"
     gene            complement(3983..4426)
                     /locus_tag="PKN85_02030"
     CDS             complement(3983..4426)
                     /locus_tag="PKN85_02030"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HNT43207.1"
                     /translation="MSSKEIVPMVEWIRSYQTLLAWLAAASTVIFIVSLALVPFFVIR
                     IPSDYFVPGKDCGHPRSDRHFVLRSIVRIGRNLLGFVLLVAGLLMLVLPGQGLLTILV
                     AAMLLNFPGKRRFERWIVSRRPVLRAINWIRRRAGRPPLLSGVGE"
     gene            4573..6054
                     /locus_tag="PKN85_02035"
     CDS             4573..6054
                     /locus_tag="PKN85_02035"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT43208.1"
                     /translation="MAEEYKALIGGEWVGSTTGETMDVVNPATGDVVAKVAKCGTAEV
                     DRAVQAARKAGPGWASKTVAERSKVLLNLSRIIMEHHEELAKLETLEHGSPIRKTMNF
                     DVPLCAEQFEYFAGIARAMTGETLPVGPWCTSMTVREPLGVVALITPWNFPALMVVWK
                     LGAALVTGNTCVVKPPSIAPLTTLKLVELAMEAGVPAGVVNVVTGPGETVGEALVSHP
                     DVAKVGFTGDTATGKRIMSIASANVKQVGLELGGKNAFVVLPDADVDSAAEGAVWAAF
                     FNSGQVCAAASRFYIHESIYDEFAEKMVALAVTLRVGDPRKMETVIGPLAYKEHRDKV
                     EWYIESGRKSGAKLLLGGQRPDTDETRKGFFVAPTIFGDCRNDMEVMREEIFGPVAGL
                     ARFKTEEEAIGLVNDTPYGLSASLWTKDVRKGLALAGRINAGTVWINEHLIIFCDTPW
                     GGCKQSGWGKDLSKMVLDEYTMTKHIYIDLTGERVKPWYGLLK"
BASE COUNT         1348 a         1799 c         1888 g         1199 t
ORIGIN      
        1 taccgcagcg cctataacgc ggcaaacaat gtgtatcaca acaagctgca aggcgtctgg
       61 accagcacct atttcaccga gaaacgcaga aaactggacg agtttgtccg cagcctggag
      121 gcagactatc agggtctgct ggatttgttg aggccgtacc gggaccagcc gtggggactc
      181 gcgatgtgga acagggtgca gcgcgattac cgcatcgccc ggaagatcag aacagacttt
      241 cccgaggaga gatgggaaca gcacctgtac ggcaggataa atgtcctgtt aagcccggag
      301 ggcaacggct gcccgcagcc gggccgcggc aagatgtgct catcgcgcga gatcgatgtt
      361 tacaacggct acgtcaggga ggtccagagt atcgggcaga ggtcatacgc tctggcgaag
      421 agtgcccagg accacgcgaa gcgcgccctc gaatgcagcg gacggatgcc cgaacccaaa
      481 ccggcgcggc catcgggcac ccaaaggcct ccaccatcac cgcccgcgga cagtgcgggc
      541 tgccgcgcca acgagcagaa atgcgggacc tggtgctgcc agcggggagt ttcctgcttc
      601 gacgcgtact ccatgaagat ggcgatatcc cagggccggt gccgaacgcc ttaggaaggg
      661 ttgccccgga gccccatcac gggtgacgcg gcgaaccgcc taatgtagtc tactttacag
      721 taaagttcgt atactctgtt ctacagtaca tttttgaggt tcaggttttg cgccgtgttg
      781 aacgacagac gtgaggcaag ggggttcgta tgaaatggtt attgcttgtc gtttttgtcc
      841 tgatgtccgt atcgcccgtt ttggcccaga cacagatgtt caacaatccc cgcgtgaacg
      901 gttatgccgt tgaccggtgt ctgtactccg gccagcagtg caaccagccc gcggctgacg
      961 agttctgccg gcgcaacggc ttccagaggg cccggcggtt tgcctgggac ttcatggcgc
     1021 cgacgctcat ccttgccacc ggccagatct gcaaggttcc gggtaggggc ggctgcggag
     1081 gtttcacctt catcgagtgc caggctcgcg gtcagggagg agggggattc gggcatggag
     1141 agcgcacctt caacaatccc cggatgaacg gttatgccgt tgaccgctgc ctgtactatg
     1201 gccagcagtg caaccagccc gcggctgacg agttctgccg gcgcaacggc ttccagaggg
     1261 cccggaactt cgcctgggac ttcatggcgc cgacgcgcat tctcgccacc ggccagatct
     1321 gcaacgtccc gggtaggggc ggctgcggag gtttcacccg gatcacctgc gtcggcggcc
     1381 ggggaggagg acccaatcca cccccgcggg gaggcaaggg cccgggcgac ccgtgccaga
     1441 ccagggagga gtgccgcagc gggatctgcc tccttggagt gtgcaattga ccccgggaaa
     1501 gagcgcgggg cgagacccgt tggtctgacc ctcggctccg tcaccgcgga gaaacgggag
     1561 acgctatttc ttcgaaaaaa acagcatcat ggggctgccc gggtggcggg acgaacccgc
     1621 gcgtcatctg aaacaggacc tgtcgataac accgaagatc ctgttgatgc gctccgacgg
     1681 cggcagggtt tcacggatca tttcaaggta acgacattcc ctgacacggt ccagtgtggg
     1741 ttggaaattg acgtcaaaga cccaccctat ctgaagcagc ttaaaatcgt tcaggttttt
     1801 tatatgagcc atgttcacta tccttttgtt catgacgtca tgatagacct cttccgagaa
     1861 tccgggcgtg tcgggcaggt caagctcgat ggctttgttg gggtctttgt ccttccgcaa
     1921 gtagtggtcc gtgaccacct tccatatgtc cagcttgtcg gcgtcgcgca ccagccttga
     1981 gtagaacagg cacgtttcgg actcgtcgga cggaagcgaa gccctgttgt ggtaccgtac
     2041 agacctgacg atgaggtctc gtaccgcgtc gtcgaaaaga tccagtatcc cggaccccat
     2101 gatcgtggcc acgcctagct cggcatggtt ctcagatctt ccatccacga aggtgcgata
     2161 gcgcgcgtat tgctcaaaac ggccgacgtc atggagcatg gctatgatct cggccaaacg
     2221 cagctcctcc tcgttcaagc ccagtcgcgt tcccagggat gatatctccg cgcacacacg
     2281 caaggtgtgc catttcttca tgtcgatgct ctgttgcctt tcggggtcgc tgtactggaa
     2341 tgtgtgaacg taatccccaa accactcctt cagttcctca actctttcac tgctgatcga
     2401 tatccgcatt tcccggtttc ccctgttatc cttcgccttg tccgaccctc ccccggttct
     2461 cagggtttcg cccacagaag gtcgttgccg aacttttgga gccaccattc ctcggccgac
     2521 aggtaccttt tgacctcttc cccggcaaag gaatacttgt attcctcgca gtcgaaccac
     2581 tcgtcgtact gtttcgcatt gctccgatac gggtcgtcgt caaccatccg cctcacaacg
     2641 tcaattccgt tgaaaacaat atgcgagggc gaacatcatg gcgaggccga cggcgaagaa
     2701 caggaaatgg agtatcgccc tcgggaggct ttcctgggcc ttgatctccg gtatgaggtc
     2761 cgaggcggca atgtaaatga agctgcccgc gccgaagagc accagccccg atacgttgaa
     2821 actccgtgac gcgatccatg cgatcagccc tcccagggga aaactcaggg ccgagaagag
     2881 attccacagc aaagcgcgac gtttgggcca gccgccgtgg acgagcacgc caaaatcacc
     2941 caactcctgc gggatctcgt gggcagccgc cgcgatccat gcgatcacac ccgccttcgg
     3001 ggaaatgagg aaggtgcttc ctattccaag ccctccaaga aagttgtgga ccgcgtctcc
     3061 gatgaggatc aggtaggtga cgggcttctt cgcttccgcg ggcttggaat gtgaatgatg
     3121 ccagtggagg aactgctcga gcgcgagaaa cgccgagaag ccgcccatga gccacaatgc
     3181 cgcgcctaaa ggggaaagga gtttgccgcc ctccgggatc atgtgaaaga aaccgccccc
     3241 gagcaacgtg ccggacgcca gagcgacgag cggcagaagt acacgctcca gaaccaccgg
     3301 tcccagcaag gtggtcaaac cacctaccag ggcgatggcg ctcatcaaaa atccgcctcc
     3361 gacaatgaaa atgaaagtgt ccatctcacg attccacgtt acatcctact tctcgccatc
     3421 ccctgctgtc aagacgacct cagcctcgtg aagcaatcgg gcgaagatgt tacgcgcggg
     3481 gtttataacg ggttagggaa acagggatgc gaaggacaca aaacacccta tccctgtcca
     3541 ccgtgcgagg gtaaacaggg ctcgaaaatg gtcctggaga aaagctctta gttcaagacc
     3601 ctatgagttt gcggcacatc gggatatgaa acggtaattg tccttcagtt cgcagggacc
     3661 ttgtcaagcg cttccgccat gtcaattccc acgaacatgg tgcctttcct gaagacggtt
     3721 ccgggtgcta ccctgattat ctttcccgtc ttcatggtta tatcggagtt ttggatacaa
     3781 acccaggagc catcgggctt cttctcaaat tccttaagat tgacttttgc tatctcgtcc
     3841 ataatcctac tcctctttaa ggtaaatgca atcatattat atagtaaaga aacaccgctt
     3901 atgtcaatag ccaaagtggt cctgtttccc ggggctgctt agacggttcg cttccgtccc
     3961 gtcccccgtt tgatgttccg cctcattctc cgacgcccga taacaggggc ggtcgcccag
     4021 cgcggcgtct gatccagttg atggcccgca gcaccggacg gcgcgagacg atccatcgtt
     4081 caaaccgcct cttgcccggg aagttgagga gcatggcggc gacaaggatc gtcaacaagc
     4141 cctggccagg caagaccagc atgaggaggc cggcgacaag gaggacgaac ccgaggaggt
     4201 tcctgccgat cctgacgatg cttctcagca cgaaatgccg gtcggagcgg gggtgtccgc
     4261 aatccttccc ggggacgaaa tagtcggaag ggatccggat cacaaagaag ggaacgagcg
     4321 cgagtgacac aataaagatc accgttgacg ccgccgccag ccacgccagg agggtctgat
     4381 aacttcgtat ccactcgacc atgggaacta tctccttact actcatagca cagaaaccac
     4441 ctctccggga agatgacccg tgggccttgt caacggctct ccgggggaga tggaggagag
     4501 tgttgtgatg accggaagtc tgtgcgaaaa tgaaggaaag ggttgtgaaa gcaaactaca
     4561 ttggaggtcg atatggctga ggagtataag gctctgatag gaggagagtg ggtgggctcc
     4621 acgacgggcg agaccatgga cgtggtgaac cccgccaccg gtgacgtcgt ggctaaggtc
     4681 gcgaagtgcg gaaccgctga agtcgacagg gcggttcagg cggcgaggaa ggccggcccg
     4741 ggctgggcgt cgaagacggt ggcggagcgt tcgaaggttc tcctcaacct ttcccggatc
     4801 atcatggagc accacgagga actggcaaag ctggagaccc tggagcatgg gtccccgata
     4861 cggaagacga tgaacttcga tgtccccttg tgcgcggagc agttcgaata ctttgccggc
     4921 atagcccggg ccatgacggg cgaaacgctg cccgtcggcc cctggtgcac gtccatgacg
     4981 gtgagggagc ccctcggcgt tgtggccctt atcaccccct ggaactttcc cgccctcatg
     5041 gtcgtgtgga agctcggagc ggcccttgtc acgggcaaca cctgcgtcgt caaaccgccg
     5101 tccatcgcgc cgctgacgac cctcaagctg gtggaactcg ccatggaggc cggagtcccg
     5161 gcgggtgtcg tcaacgtggt caccggcccg ggggagacgg tgggagaggc gctcgtctca
     5221 caccccgatg tcgcgaaggt agggttcacc ggggacacgg ccacgggaaa acggatcatg
     5281 agcattgcca gcgcgaacgt gaagcaggtg gggctcgagc tgggcgggaa gaacgccttc
     5341 gtcgtccttc ccgatgccga cgtcgattcc gccgcggaag gggcggtctg ggccgccttc
     5401 ttcaactcag gccaggtctg cgccgccgcg agccgtttct acatccacga atcgatatac
     5461 gacgaattcg cggagaagat ggtggccctg gccgtaacgc tccgcgtcgg cgatcccagg
     5521 aagatggaaa cggtgatagg gccactggcg tacaaagagc accgcgacaa ggtcgaatgg
     5581 tacatcgaga gcggcaggaa gagcggcgcg aagctcctcc tcggcgggca gcgtcccgac
     5641 acggacgaga cgagaaaggg cttcttcgtg gcgccgacca tattcggtga ttgcaggaac
     5701 gatatggaag tgatgaggga agagatcttc ggccccgtcg ccgggctcgc gcggttcaaa
     5761 acggaggagg aggccatcgg cctcgtcaac gacaccccgt acggtctctc ggcgtccctg
     5821 tggacgaaag acgtccgcaa gggcctcgcg ctggcgggtc ggataaatgc cggcactgtc
     5881 tggatcaacg aacacctgat catcttctgc gacacaccct ggggaggatg caagcaatcg
     5941 ggttggggaa aggacctgtc gaagatggtc ctcgacgagt acaccatgac gaagcatatc
     6001 tatatcgatc tcacgggcga acgcgtcaag ccctggtacg gactgctgaa gtgagggaaa
     6061 gcgacgatct gcctttttag ccagacgggg taaacttgat tggcaaggcc gttacggcgg
     6121 ggtggcgtgg ggacgagaca atgaaatggt agacggccgc ggtataaagt attcaacctg
     6181 gtgcgcgtgt gcactggtca ttgcactcgc gctgtggctt cctcacaccg cggg
//
LOCUS       DAOQTR010000049         6167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14093_length_6167_cov_4.310373, whole genome shotgun sequence.
ACCESSION   DAOQTR010000049 DAOQTR010000000
VERSION     DAOQTR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14093_length_6167_cov_4.310373"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKN85_02040"
     CDS             <1..346
                     /locus_tag="PKN85_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43209.1"
                     /translation="VKEIECIQMELGEPDASGRRTPVEKKGSNFRMQTDAVVCAIGNS
                     PNPLVPATTPGLDTTKRGTLVADPETGKTTRDRVWAGGDIVTGAATVILAMGAGRKAA
                     RAIHEYLTTGKQ"
     gene            complement(807..1403)
                     /locus_tag="PKN85_02045"
     CDS             complement(807..1403)
                     /locus_tag="PKN85_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNT43210.1"
                     /translation="MIVREESYGKRVVLFPGDYFVTDKKVRISTLLGSCVSACLYDPV
                     NRVVGMNHFLLSTKRYTDDVPLVLSEAGRYGVHAMEIIINGMLKLGAQRRYLKAKVCG
                     GSSLLKTQKDEDNFRCVGRVNCAFIQEFLRNDGIAIVSSDLGGDLGRVVHFDTHDYAL
                     YIRKIRKVSLQGLGARDKEYWKRELKRHLDEGTTPDIW"
     gene            1654..2160
                     /locus_tag="PKN85_02050"
     CDS             1654..2160
                     /locus_tag="PKN85_02050"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNT43211.1"
                     /translation="MESVFTETKAEDIGRNPFRLIGADWMLITAGTKDSFNTMTGAWG
                     GFGIVWDKRVAICLVRPTRYTYEFMERSWSFSLCFFDEQYRDALTYCGTTSGRDVNKV
                     AEAGLTPVFGEDTTYFAEARLVIECRKIYRQDIEPQNFLAPEIDGFYPKKDYHRMYVG
                     EIIRCLSR"
     gene            2333..2692
                     /locus_tag="PKN85_02055"
     CDS             2333..2692
                     /locus_tag="PKN85_02055"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT43212.1"
                     /translation="MRLEEEMEVFELQAEVCLALGNPRRLQILGLLKEGERSVAQMLE
                     RMGINKANLSQHLAVLRQKGLVISRREGGAVYYRLASPRITEACSIMREVLLETLRSR
                     ERISRSMLEGMGTVGEG"
     gene            2757..3449
                     /locus_tag="PKN85_02060"
     CDS             2757..3449
                     /locus_tag="PKN85_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT43213.1"
                     /translation="MGGEMGKGSAREGLERAKRSRRAKQVVLGTVFLALLVGGWFYSL
                     IGYFIPLCMVAGVGLASVRGRKWCNWMCPRGSFADTYMKLISPERKIPGWLRGTPVRL
                     GVLAFLMAMLAFQIVRLWPDPYAIGRFFVILLTVTTVVGVFMALFLHQRAWCSICPIG
                     SLSSWVGKDRYQLNMEKDACVNCGLCAKTCPMQLAPQEMKENEAMAFKGDCLKCGLCV
                     KACPKQALRFPG"
     gene            3712..5838
                     /locus_tag="PKN85_02065"
     CDS             3712..5838
                     /locus_tag="PKN85_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNT43214.1"
                     /translation="MNTVRDLKSEHCRGDSRVEVNNRKCRRCFYCVQLCPAKAIKLEK
                     ESIRIIPERCILCGNCITGCPHRAMTYHSDASIVRNLIEANENVVACLDPAFPAVLDN
                     VSPWGFSYALKALGFKEVWEGAVGAELISGPYRKLLTETEKPVISSFCPVIVSYIEKY
                     LPQLIGNLAPIVSPMIAAGRAIKKAKGEGWRVVYIAPCLARMGELDYEENADAIDHVI
                     TFRDVRFMFAEAGINPRHVDEAPFDGPVPALGRIVPVIGGLNRSLGHSFDVLVGETSV
                     AYGRRRVMAALNQLAKGAIQARFFDLVFCDGCVDGSFVERELSVVGRRQIVSRYAKSQ
                     FSPQTASVLQRTLRDFADVKITKEHKANVQKLPDPTEEQIRAVLKRINKLPPHNNLDC
                     RACGYNTCREKAVAVVQGISEAEYCLPYLLEGSKRVYEQLEKSHRELQRSHQELEQAQ
                     FQLIRTEKLAALGQLAAGVAHEINNPLGTITIYAHILARSLEPDDPRKEDIDLIVTEA
                     NRTKEIVQGLLSFARETKLKPGETNINDLLEETLGLLVNQVLFQNIKIKKVFNDDLPL
                     LFADATQLKQVFLNIMLNAAQAMEGKGSLTLTTTHEGGTIEIRIRDTGPGIPPENIGK
                     LFNPFFTTKEKGTGLGLAISYGIVERHSGQIDVETELGKGSTFIITLPVDADAAGIIA
                     PAKGLAANQNINTGRRNHGTKKNSHH"
     gene            5810..>6167
                     /locus_tag="PKN85_02070"
     CDS             5810..>6167
                     /locus_tag="PKN85_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43215.1"
                     /translation="MAQKRILIIDDDVDFVDLNKAVLQNNGFEVETAFSAREGMDKVQ
                     FEAPDLILLDLMLEKHDTGFSFAKAIKADPRYKTIPILMISAVAGETGYDFTQEQDGY
                     WMKTDDFVAKPVEPEEL"
BASE COUNT         1386 a         1703 c         1856 g         1222 t
ORIGIN      
        1 ggtcaaggag atcgaatgca ttcagatgga acttggcgaa cccgatgcct cgggacggcg
       61 cacccccgtc gagaagaagg gttccaattt ccggatgcag acggacgcgg tggtatgcgc
      121 catagggaac agccccaatc ccctggtgcc ggcgactacg ccgggccttg acacgacgaa
      181 acggggaacg ctcgttgccg accccgagac gggaaaaacg acccgcgaca gggtgtgggc
      241 gggaggcgac attgtgaccg gtgcggcgac ggtcatcctc gccatggggg ccggcaggaa
      301 ggcggcccgc gccatacacg agtacctgac gacgggaaaa cagtgaaaca cagcttgagc
      361 cgcttgaaca gctcgagcgt ggagaagaca agaaagggga atacggtttg gactgcttga
      421 gccgttcgaa ccgcttgaac gtgcagagga cgtagagacc gtcattctat tctttgtgtc
      481 caatgtgttc agtcgcgacg cgacgttatg gaacgtgggg gggcgcgaag acaccggggc
      541 tcgcgatgat gggccgcggc tgttgcacga ggggggtttt tatatacgcg ctgtcatgcg
      601 ttcaagcggt tcgagcggct caggcaaaaa ccggccgcat gtcacaggtc acacgtcaca
      661 agtctaaagg caaaaaaaga tccgctgccc gctccgcgcg ggtcagtgat tgtgttgaca
      721 acccatcccc tttcgccgtc taattttcgc aactcgcaac tcgcaactcg caactcgcaa
      781 ctcgcaactc gcaacggtct ttgtcttcac catatatccg gcgtcgttcc ctcgtcgagg
      841 tggcgcttga gttcccgttt ccagtattcc ttgtccctcg caccgagacc ctgaagcgag
      901 accttcctga tctttctgat gtagagggca taatcgtggg tgtcgaaatg gaccaccctg
      961 ccgagatctc cgcccaggtc cgaggagact atggcgatgc cgtcgttgcg gaggaactcc
     1021 tggataaagg cgcaattcac tctgccgacg caccggaaat tgtcctcatc tttctgcgtc
     1081 ttgagcagcg aactgccccc gcagaccttt gccttgaggt acctgcgctg ggcgccgagc
     1141 ttcaacatgc cgtttatgat gatctccatg gcgtgcacac cataccggcc cgcctcggag
     1201 aggacgagcg gcacatcgtc ggtgtagcgc ttcgtgctga gcaggaagtg gttcatccct
     1261 acgacccggt tgaccgggtc atagaggcac gcggagacgc aggagccgag aagggtggat
     1321 atcctgacct tcttgtctgt cacaaaataa tcgccgggaa acaagacgac ccgcttcccg
     1381 taagattcct cgcggactat catttctcat tgccaccttt acctccaccc tgcaggagcc
     1441 cccggaactg tacccctgca cactagtaat cggctgaccg gcgggaagct taaacggcga
     1501 aaataatgtt ttaggtttca agttttaggt tttaagtaaa aggagccggg atggcagagg
     1561 aagacagccg tggccttgca atcatgacca ccgcgtaata gtattgaact taaaacgtaa
     1621 cacctaaaac tcaaaacatc tttcggaggt tccatggaga gcgtgtttac ggagacgaag
     1681 gcggaggata tcgggcgcaa ccctttcaga cttatcgggg cggactggat gctgatcacg
     1741 gcgggtacga aggattcctt caacacgatg accggcgcct ggggcggttt cggcattgtg
     1801 tgggacaaac gcgtcgccat atgtctcgtt cgtccgaccc gctataccta tgaattcatg
     1861 gagcgttcat ggtcgttcag cctgtgcttc ttcgatgagc agtaccgtga cgccctgacc
     1921 tactgcggga caacgtcggg cagggacgtg aacaaggtcg ccgaagcggg tctcaccccc
     1981 gtcttcggcg aggacacgac ctacttcgct gaggcgcgcc tcgtcatcga gtgcaggaag
     2041 atctaccgcc aggacataga gcctcagaac ttcctcgcac cggagataga cggcttctac
     2101 ccgaaaaagg actaccacag gatgtacgtg ggcgagatca tccgctgcct gtcgaggtag
     2161 gagaaacggg aatctccccg tgtcctatcc ggtcttagag ccctcaccac ttcaccccgg
     2221 tctgccctgc cctcccaatc cccgacccag ccgggtcttg acagcaggca caggttgtat
     2281 tatagttgcg atatcatgaa actacgcaat taggaaattg aaggagatgg tcatgaggct
     2341 tgaggaggaa atggaggtgt tcgagttgca ggctgaggtg tgcctggcgc tggggaatcc
     2401 gcggcggctg cagatactgg gcctgctgaa ggagggggaa aggtccgtgg cgcagatgct
     2461 ggaaaggatg gggataaaca aagcgaacct gtcccagcat ctcgcggttc tgagacagaa
     2521 agggctcgtc atatccagga gggagggcgg ggcggtgtac tacaggctcg caagtccgag
     2581 gataactgag gcttgctcga taatgcgcga ggtgctgctg gaaaccttgc ggagtagaga
     2641 gcgcatttcc cgaagcatgc ttgaagggat gggaactgtg ggagagggat aggacgtata
     2701 ggagttgata ggaggaccga taggacatat gggacctata ggacttggag ggagttatgg
     2761 gtggtgagat ggggaagggt tcggcgaggg aaggacttga aagggcgaaa cggtctaggc
     2821 gtgccaaaca ggttgttctg ggaaccgtct ttcttgcgct tcttgtcgga gggtggttct
     2881 attctctgat cgggtatttc attccgctgt gcatggtggc tggtgtggga ttggcctccg
     2941 ttcggggcag gaagtggtgc aactggatgt gcccccgggg gtccttcgcc gatacctata
     3001 tgaagctcat cagtccggag cggaagatac caggctggct gaggggcaca ccggtgaggt
     3061 tgggcgttct ggcattcctc atggcgatgc ttgccttcca gatcgtgcgg ctgtggccgg
     3121 acccgtacgc gatagggagg ttttttgtca tactactcac cgtcacgacc gtcgtggggg
     3181 tcttcatggc gcttttcctt caccagcgcg cctggtgttc catctgcccc atagggagcc
     3241 tatcgagctg ggtcgggaag gaccgctacc agctgaacat ggagaaggac gcctgcgtga
     3301 actgcggtct ctgcgcgaag acctgcccta tgcagcttgc cccccaggaa atgaaggaaa
     3361 acgaggccat ggccttcaag ggcgattgcc tcaagtgcgg cctctgcgta aaagcctgcc
     3421 cgaaacaggc ccttcgcttt ccgggataag gacggttcaa gggttcaaaa gttcaagagt
     3481 tctaagggta aaagacaaaa aaagcaccgc aggccttggg ttagtgtgcg gatcgtcttg
     3541 acagcacaat cactcagcgg ggcacgtcat ggttcttcac ttgaaccctt gaacttttga
     3601 acccttgaac cttctttgag gcccttgggc cagccctgtt tccattcatt gacaaagcct
     3661 cccaattcct gtaagatttg tggtaattta ttaaattttc tgtgtatttg gatgaatacc
     3721 gtacgcgatc tcaagtcaga acactgccgg ggagattcca gggtagaggt caacaaccgc
     3781 aagtgccgca gatgctttta ctgtgtgcag ctctgtcccg cgaaggccat caaactcgaa
     3841 aaggagtcga tccgcatcat cccggagcga tgcatactct gcggcaactg catcaccgga
     3901 tgtccgcacc gtgcgatgac atatcacagc gacgcgagca tcgtcaggaa cctcatagag
     3961 gcgaatgaga atgtcgtcgc ctgcctcgac cccgcctttc cggccgttct cgacaatgtg
     4021 agcccctggg gtttcagtta cgccctcaag gccctcgggt tcaaggaggt ctgggaaggt
     4081 gccgtcggtg cggagctgat aagcggacct taccggaaac ttctgacgga aacggaaaaa
     4141 cccgtcatat cttccttctg ccccgtcatc gtctcctaca tcgagaagta tcttccccag
     4201 cttatcggta atctcgcgcc catagtctcg ccgatgatag ctgctgggag ggccatcaag
     4261 aaggcgaaag gggaggggtg gcgcgttgtc tacatcgccc cgtgccttgc ccggatgggg
     4321 gagttggact acgaggagaa cgcggatgcc atcgatcatg tcatcacctt tcgcgacgtg
     4381 cggttcatgt tcgccgaggc cgggatcaac ccgagacacg tggacgaggc gcccttcgac
     4441 gggcccgttc cggcactggg ccggatcgtc cccgtcatag gcgggctcaa ccggagcctg
     4501 gggcacagtt tcgacgttct cgtcggggag accagcgttg cctacgggcg aaggcgggtc
     4561 atggccgcgc tcaaccagct tgcgaagggg gccattcagg cccgtttctt cgatcttgtt
     4621 ttctgcgatg gctgcgtgga cggttccttc gtggagcgag agctttccgt ggtgggaaga
     4681 aggcagatcg tatcgcggta cgccaagtcc cagttcagcc cccagacggc gtcggtgctt
     4741 cagcgtaccc tcagggactt tgctgatgtg aagatcacga aggagcacaa ggccaacgtt
     4801 cagaagctcc ccgatccgac ggaggagcag atacgggccg tcctgaagag gataaacaag
     4861 ctgcctcccc acaacaacct cgactgccgg gcctgcggct acaatacgtg ccgagagaag
     4921 gcggtcgccg tcgtgcaggg catatcggaa gccgagtatt gcctgcccta ccttctggaa
     4981 ggctcgaagc gcgtctacga gcagctcgaa aaatcccaca gggagctgca gcgctcccat
     5041 caggaactgg agcaggcgca gttccagctc atcaggacgg agaagctcgc ggccctggga
     5101 cagctcgccg ccggcgtcgc ccacgagata aacaaccccc tcggcacgat caccatctat
     5161 gcccacatac tggcacggtc cctcgagccc gacgacccga gaaaggaaga catcgacctc
     5221 atcgtgaccg aggccaacag gacgaaggag atcgtccagg ggctcctgag cttcgcgcgg
     5281 gagacaaagc tcaaaccggg cgagacgaac atcaacgacc ttctcgagga gaccctcggt
     5341 ctgctcgtca accaggtcct ctttcagaac atcaagatca agaaggtgtt caacgatgat
     5401 ctccccctgc tcttcgccga cgcgacgcag ctcaagcagg tgtttctcaa catcatgctc
     5461 aacgcggcgc aggcgatgga gggcaaaggc tcgctgacgc tcaccaccac ccacgaggga
     5521 ggcacgatcg agatcaggat acgcgacacg ggaccgggca tcccgccgga gaacatcggc
     5581 aagctcttca atcctttctt taccacgaag gagaagggga cggggctcgg actcgccatt
     5641 tcctacggca tcgtggagcg ccattcgggg cagatagatg tcgagacgga actgggcaag
     5701 ggaagcacat tcatcatcac gttgcccgtc gacgccgatg cggcggggat cattgcaccg
     5761 gccaaaggat tagcggcaaa tcaaaatata aatacaggga gaaggaacca tggcacaaaa
     5821 aagaattctc atcattgatg acgacgtcga cttcgttgat ctcaacaagg cagtcctgca
     5881 gaacaacgga ttcgaagtcg aaacggcatt ctcggctcgc gagggcatgg acaaggtgca
     5941 gttcgaagcg ccggacctca tcctcctcga cctcatgctg gagaaacacg acacggggtt
     6001 cagtttcgcg aaggccatca aggccgatcc gcgctacaag accatcccca tcctgatgat
     6061 ctccgccgtt gccggcgaga cggggtacga tttcacccag gagcaggacg gctactggat
     6121 gaagacggat gatttcgtag ccaagccggt ggagccggaa gaactgg
//
LOCUS       DAOQTR010000050         6165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14098_length_6165_cov_3.930933, whole genome shotgun sequence.
ACCESSION   DAOQTR010000050 DAOQTR010000000
VERSION     DAOQTR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14098_length_6165_cov_3.930933"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PKN85_02075"
     CDS             complement(<1..926)
                     /locus_tag="PKN85_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNT43216.1"
                     /translation="MKNVRMALAGLMAGLMLVLAIEAHAADPDTVYIRHLEGIVELAE
                     AGSPQWMEAAVNTPLIAGDTVRTAASGKAELFLKDGSVVRVGKNSVMRIVAVEPKGVQ
                     FRLDRGGAYIVARGSKDVPIFFDTPSAALDVTQPTTVRVDVYDGGISEVSVYQGEVQA
                     LQQKGRMPVRAGERLALAADGSIPRVTGLRSADEWQRWNTERDRATFAAVSTGESTAY
                     LPEELRTYSSDLDANGQWVYTPEYDYVWVPTVITVSTWSPYRFGRWVWIGGSYVWVGY
                     EPWGWAPYHYGRWVHHRHAGWCWVPPTRGHVR"
     gene            1164..5015
                     /locus_tag="PKN85_02080"
     CDS             1164..5015
                     /locus_tag="PKN85_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNT43217.1"
                     /translation="MKRFRSYRLMMLLTAAAVMFVLSPAQREALAFSAVQNLDLDTHG
                     IILRGAWEQLSKDPVFKDSNFPRIDGISRFEGVFKKASDPFATGGGGPDVKGRTRDSE
                     HYYNPKITGKNKGGAPASVGKYYSILVERMLGGQGGVSPEQGAAWAAHFLADMNVPYH
                     IVGIPAGGAEAIYVGGIPGRTAHSLTSDESGPLWLKEVPLTYDRLWKAFTFDFYRDWP
                     GWGTTQDFERTIAVFHTVRERAGSERDLHDWFDPWYYNGWGMGMEPFQTNPVVHNSSH
                     AKWEVWAHLYLARWTNYKPSVNTGFHPQWRNGWVTFNDKVPWEAQKAQAEEFAKKAAE
                     QTWDEMQQVYRSPDIATDDAVWRVMTLWRASISAMKPSMEARQDLTNPARHELRAIID
                     NREKENVFDVKVRLTLTGDGSTFSEVKDITGSIAAAGKSEVTFTIEPKDPAKCKVRLE
                     VIGHYEKTPDLQYARIEEPFGGIRRSADEAQNTALRGILDSLKQAAEKAERLSTEIAA
                     LCSASSTAIEAAQADLKKLGARVESMEEEVKSTEANAEAMKEKAESIRQFHLDAEEGA
                     TNEGKVAHEAESVSKELCEATKAVQNAQSGEERGQLFAGMESTKDELKVFLAEGNSLL
                     EKVKRTDRSSQTTLKELEGLAKDPGKSVQRADAQVSPETLGRKFLDIAEKIASARQEM
                     KDLDAVEEGARKKYGEGERILASLQGTAGIDKIRTDMDGYMDRVTKARSQVRDCPGKV
                     QAALEELRRDAVPVNEAYTKASQSIAGLIPGANTHADTLAKARETAGLTTYLSGMTGA
                     YMERIRRACTEGAFCFTLAQDAMRRPVYVIVPNVKERHVDNAVGILKTKNFKPRPSVV
                     GAAPKIDLEFKVQSQSPGGDTKAPEGSPVTVYYYGQFNTQDAARNADCSRWPGSVGKW
                     DAVKKRVICVCPEGASWSRTRTSCVSDREAALAGIDCSRYPGSVAVFDERTKKAGCQC
                     GQGLAWDKSRTRCVDTRTAALERADCSRFPGTLPAWNQQSGKVECFCPQGSLWDKGTR
                     RCVSRQELDTYCNQMGARLTAAARANNIRAFRELLPLVQDCDFYNQALADLRTMEYNQ
                     QMAMMNQMANMMGAMSGGMRPPQQPLPGASPGGTGTAPIRPATPTVSPVRPPAATTTQ
                     PGSVKPPQTTGGGSKVDCDKKYCPICGNDNIDLLRTSVSQQCMDCRKRFATQIADCNR
                     GGPAASTPGATVAQFQNHYVLKCVRQRWNAATKRYDTVTVYAVYGPGKQYPSGNCGRV
                     YGPDTESQCNMRAEAWSRRVR"
     gene            5040..5792
                     /locus_tag="PKN85_02085"
     CDS             5040..5792
                     /locus_tag="PKN85_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNT43218.1"
                     /translation="MKARSFSTGGITAAAALVLLAAVSFVANLQAAAPLGGQVAAVHG
                     TNIEIIVASKGAIEPGDRVELLYTTSTGVELPVGIWRVRTVSGTTVTAEMADSATRPV
                     VGLRAKIYPGSKVDEKTTQAPMPSVASPERPQTSSLPPPAATGWLGVAIVADQKGGPD
                     GDSDMLSRFFGANPGAGVAVAEVAAGGPADRAGLNVQDLIIELNGQPAGKAADFARSI
                     GDLGEGATAKLTIIRQGKRLVIPVILERAPGH"
     gene            complement(5825..>6165)
                     /locus_tag="PKN85_02090"
     CDS             complement(5825..>6165)
                     /locus_tag="PKN85_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43219.1"
                     /translation="VASQMVAEGLLPVDGLIFLGYPLHPANDMGRLRDSHLYMIEVPM
                     LFFAGTRDPLCSLPLLKGVLERLRAPREILTIEGGDHSFHVPKSLHRQEEEIFAEITA
                     TAVEWIGRLK"
BASE COUNT         1384 a         1845 c         1917 g         1019 t
ORIGIN      
        1 cagcggacat ggccccgcgt cggcgggacc cagcaccatc ccgcgtgacg gtgatggacc
       61 caccgtccgt aatggtacgg cgcccaaccc cagggttcgt atccaaccca cacgtagctt
      121 cccccgatcc agacccagcg accgaaacga tagggagacc aggtactgac ggttatcacc
      181 gtagggaccc agacgtagtc gtactccggc gtgtagaccc attggccgtt cgcgtcgaga
      241 tcggacgagt aggtcctgag ttcctcgggc aggtaggcgg tactctcacc ggtggacacg
      301 gccgcgaatg tcgccctgtc cctctcggtg ttccacctct gccactcgtc ggcagagcgc
      361 agcccggtga cacggggaat ggatccgtcc gcggcgaggg cgagacgctc cccggctctg
      421 acgggcattc ggcccttctg ctgcagcgcc tgcacttcac cctgatagac ggacacctcg
      481 ctgatcccac cgtcgtatac atccacccgc accgtcgtag gctgcgtgac gtcgagcgct
      541 gctgacgggg tatcgaagaa gatcggaaca tcctttgacc cacgggcgac gatgtacgcc
      601 cctccccggt caagcctgaa ctgaacgccc ttcggttcca cggcgacgat cctcataacc
      661 gagttcttcc ccacacgaac cacgctcccg tccttcagga agagctcggc cttccccgac
      721 gccgccgtcc ggacggtgtc gcccgcgatg agaggcgtat tcacggcagc ctccatccat
      781 tgcggtgacc cggcctctgc aagctccacg atgccttcga ggtgtcgtat gtatacggtg
      841 tcagggtcag ccgcatgggc ctcaatggca aggacaagca tcaatcccgc catcaatccc
      901 gctagtgcca tacgtacgtt tttcatatgc cctctcctca actttgatcc cttcttgaga
      961 gcaagatgcg taccagcagg ttgattcgct ttaacccatt ataactattg tcattttatt
     1021 gacgatactg cctcaggagg ggaaatatct gtcgacggga ccttctggca acgacaatac
     1081 cgtcgaagac aagggaggcc gttttgggtt gaaaaaaatg ccataaaacg taagaattgg
     1141 aggaaaatgc ttcggaggcc cttgtgaaaa ggttcagatc gtatcggctc atgatgcttc
     1201 tcaccgctgc ggctgtgatg ttcgtcctgt cgccggccca gcgtgaggca ctggcgttca
     1261 gcgccgttca gaacctcgac cttgacaccc acgggatcat cctacgcgga gcgtgggagc
     1321 aattgtcaaa ggaccccgtc ttcaaagaca gcaactttcc ccggatcgat gggatctcca
     1381 ggttcgaagg ggtttttaag aaagcgtcgg atcctttcgc aaccggcgga ggaggccctg
     1441 acgtcaaggg gcgcacgagg gactcagaac actattacaa tccgaagatc acgggaaaga
     1501 acaagggcgg ggctccggca tcggtcggca agtattactc gatccttgtg gagagaatgc
     1561 tcggcggaca gggaggggtt tcccccgaac agggcgccgc ctgggccgca cattttctcg
     1621 cggacatgaa tgttccctac catattgtcg gcatacccgc gggaggggcc gaggcaatct
     1681 acgtgggagg catccccgga aggaccgccc acagcctcac gtctgacgag tccggtccgc
     1741 tgtggctcaa ggaggtacct cttacctacg acaggctgtg gaaggccttc acttttgatt
     1801 tctaccggga ctggccggga tggggaacga cacaggattt cgaacggacc atcgccgtct
     1861 tccacacggt gagggaacgt gccggctccg aaagggacct tcacgactgg ttcgacccct
     1921 ggtattacaa cggctggggg atgggcatgg aacctttcca gacgaaccct gtcgttcaca
     1981 attcgagtca tgcgaagtgg gaggtctggg cccacctcta cctcgcccgg tggaccaact
     2041 acaagccctc cgtcaatacg ggatttcatc ctcagtggag gaacggatgg gtgactttca
     2101 atgacaaggt gccctgggaa gcccagaagg cacaggctga agagttcgcg aaaaaagcgg
     2161 cggagcagac ctgggacgag atgcagcagg tataccggtc gcccgatata gcaacggatg
     2221 acgccgtatg gcgcgtaatg acactgtggc gggcatccat atctgcgatg aaaccgtcca
     2281 tggaagcaag gcaggacctg acgaaccccg cgcggcatga actccgggcg atcattgaca
     2341 acagggaaaa agagaacgtc ttcgatgtaa aggtgcggct gacattgacc ggtgacggca
     2401 gcaccttcag cgaagtgaag gacatcacgg ggagcatcgc ggctgcggga aagagcgaag
     2461 tgaccttcac catcgagccg aaagacccgg cgaaatgcaa ggtccgtctc gaggtgatcg
     2521 gtcactatga aaagaccccc gacctccagt acgccaggat cgaagagccc ttcgggggca
     2581 tacgcaggtc cgccgatgaa gctcagaaca ccgctctgcg gggaatcctt gacagcctga
     2641 aacaggcggc ggaaaaggcc gagcgcctat cgacggagat cgccgcgctg tgcagcgcct
     2701 catcaacggc catagaagcg gcgcaggccg atctcaagaa gcttggagcc cgcgtcgagt
     2761 ctatggaaga agaggtcaag tccacggaag caaacgcaga ggccatgaag gagaaggccg
     2821 aatcgatacg acagttccat ctcgacgcgg aggagggcgc aaccaatgag ggcaaggtcg
     2881 ctcatgaggc ggaaagtgtg agcaaagagc tgtgcgaggc cacgaaagcg gtccaaaacg
     2941 ctcaaagcgg ggaggagcgc ggacagctct ttgcgggaat ggagtccacc aaagacgaac
     3001 tcaaggtgtt ccttgcggag ggaaactcgc tcctcgaaaa ggtgaagcgg accgaccggt
     3061 cctcgcagac aacgttgaag gagctggaag gtctggcgaa ggacccgggg aagtccgtgc
     3121 agcgggcgga cgcacaggta tcaccggaga cgctgggcag aaagtttctg gacatagccg
     3181 aaaagatcgc ttcggcgcgg caagagatga aggatctcga cgcggtcgag gaaggggccc
     3241 gcaagaagta cggcgagggc gagaggatcc tcgcgtcgct tcaggggacg gccggaatcg
     3301 acaagatccg caccgatatg gacgggtaca tggaccgcgt aacgaaggcg cgcagccagg
     3361 tccgggattg ccccggcaag gtacaggcgg ccctggagga gctccggcgc gacgcggttc
     3421 ccgtcaatga ggcttacaca aaggccagtc aatccattgc cggcctgatc ccaggggcga
     3481 acacccatgc agacacgctc gcgaaagccc gcgagacagc aggactcaca acatatcttt
     3541 cgggcatgac aggggcctac atggaacgca tcaggagggc atgcaccgag ggggccttct
     3601 gtttcaccct tgcccaggac gcgatgaggc ggcccgtgta cgtgatcgtg cccaacgtaa
     3661 aggaaaggca tgtcgacaat gccgttggaa tcctgaagac aaaaaatttc aagccgcggc
     3721 caagcgtggt cggagcggcg ccgaagatcg acctcgaatt caaggtccag tcccagtcgc
     3781 cgggcggcga caccaaggct cccgaaggca gtcccgtgac ggtctactat tacggacaat
     3841 tcaatacaca ggatgccgcc cggaacgccg actgttcacg atggcccgga agtgtcggaa
     3901 agtgggacgc tgtaaagaaa cgggtcatct gtgtctgccc cgagggcgcc tcgtggagcc
     3961 ggacccgcac gtcatgtgtc agcgaccggg aagcggccct tgccggcatt gactgttcta
     4021 gatatcccgg ctccgtcgcc gtatttgacg agaggacaaa aaaggcggga tgccagtgcg
     4081 ggcaggggct ggcctgggac aagagccgta cgcgctgtgt cgacacccgg acggcggccc
     4141 tcgaaagggc ggattgctcg cgctttcccg gaacgctgcc cgcctggaac cagcagtcgg
     4201 gcaaggtcga atgcttctgc cctcagggtt ccctgtggga caagggcacg agacggtgcg
     4261 tcagccggca ggaactggac acctactgca accaaatggg cgcccggctc acggctgcgg
     4321 ccagagccaa caacatcagg gccttccgcg agcttcttcc cctggtgcag gactgcgatt
     4381 tctacaacca ggcgctggcc gatcttcgca ccatggaata caatcagcag atggccatga
     4441 tgaaccagat ggccaacatg atgggggcca tgtcaggcgg gatgagaccc ccgcagcagc
     4501 ccctcccggg ggcatccccg ggaggcacgg ggacagcccc catacgccct gcgacgccca
     4561 cggtctcccc cgtccggccc ccggcggcca caacaacgca gccgggctcc gtgaaacccc
     4621 cgcagacgac gggaggaggc agcaaggtgg actgcgacaa gaagtattgt cccatatgcg
     4681 ggaacgacaa catcgatctt ctgagaacat ccgtttcaca gcagtgcatg gactgcagaa
     4741 aacgtttcgc aacgcagatc gccgactgca accgcggggg ccccgcggcg agcacccccg
     4801 gggcgacggt ggcgcaattt caaaaccact acgtgcttaa atgcgtccgg caaagatgga
     4861 atgccgccac aaaacgctac gacacagtca cggtctatgc cgtgtacgga ccgggaaaac
     4921 agtatccttc gggcaactgc gggagggttt acggtcctga cactgaatcg cagtgcaaca
     4981 tgcgggccga agcgtggtcg aggcgtgtgc ggtagcgcca ggcaatacag gaggaaccaa
     5041 tgaaagcacg ttcgttcagc acaggcggca tcacagctgc agcagccctt gtgctgctgg
     5101 cggcagtctc ctttgtggca aaccttcagg ccgcggcacc gttaggggga caggtggcgg
     5161 ccgtacatgg aacgaatata gagatcatcg tggcgtccaa aggcgccatc gagccaggcg
     5221 acagagtgga gttattgtac actacctcga cgggcgtcga gcttcccgtg ggtatctggc
     5281 gcgtcaggac cgtcagcggg acaacggtga cggccgaaat ggcggacagc gcgacccggc
     5341 cggtggtggg cctcagggcg aagatctacc ccggaagcaa ggtcgacgag aagacaaccc
     5401 aagccccgat gccctccgtg gcctcgccgg agagacctca gacgtcctcg ctcccgcccc
     5461 ctgccgccac cggctggctg ggcgttgcca tcgtcgcgga ccagaaagga ggtcccgacg
     5521 gagattcaga catgctctcg cggtttttcg gcgcaaatcc cggcgccggc gtcgcggtgg
     5581 cagaggttgc agccgggggc cccgccgaca gggcaggtct caacgtgcag gaccttatca
     5641 tcgaattgaa cgggcagcct gcagggaagg ccgccgactt cgcccggtca ataggcgatc
     5701 tgggcgaggg ggcaacggcg aagctcacca tcatacgcca gggaaagagg cttgtcattc
     5761 ccgtcattct cgagcgagct ccgggccact aacggacctc cgggagagac ggtgatcatc
     5821 ctcctcactt cagacgaccg atccattcca ccgccgttgc ggtgatctcg gcgaagatct
     5881 cctcttcctg cctgtggagg gacttcggga catggaagga atggtctccc ccctcaatgg
     5941 taagaatctc ccggggtgcc ctgagccgtt cgagaacccc tttgagaagg ggcaggctgc
     6001 acagggggtc tctcgtcccc gcgaaaaaaa gcatgggaac ctcgatcatg tagaggtgcg
     6061 aatccctcag ccgccccatg tcgttcgccg gatggagcgg gtacccgaga aagataagcc
     6121 cgtctaccgg caggagtccc tccgcgacca tctgcgaggc caccc
//
LOCUS       DAOQTR010000051         6152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14143_length_6152_cov_3.767263, whole genome shotgun sequence.
ACCESSION   DAOQTR010000051 DAOQTR010000000
VERSION     DAOQTR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14143_length_6152_cov_3.767263"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PKN85_02095"
     CDS             <1..1221
                     /locus_tag="PKN85_02095"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT43220.1"
                     /translation="LKPAGYIGTPSFLKIILEKGDEGTFGRLKEKIGDELYEHLLGSA
                     AENRQRPVALVRMLTHFRMPETCGAILDILKEMDPDDENYEMVLTLFASLSEVWAADA
                     GGYMERDEANLLPLVRACKMTGVKVGEAALLRHFLTAPVEVKREIVMSVPAVVDGSGC
                     SIIKEALKDTDGHIKGFAVDAVGTLKLTNLKEDIVRILREDFHDVRVKALKTLIRLDP
                     TLAVEMIAAFVDRGSTDDKKVYLAATGLIDGQKNLPFITRLLGDEDEGVRRSTIAVLG
                     NFVDDENYMGLLHDELMGDEIPHEVLKVVKDRRLTRFKDRLVGIFADEGKGMWTRYYA
                     LSALGTFEDPALFDIFVRGLGDDNGLITIGCIRALADLNDERAVDHIRPYLVSGNDDI
                     RSAAEMVVNRTQNA"
     gene            1231..2055
                     /locus_tag="PKN85_02100"
     CDS             1231..2055
                     /locus_tag="PKN85_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNT43221.1"
                     /translation="MTKEEFTVLRDFIYEKTGIYFTETKTYLLESRLTNRLNELGLGS
                     FEDYYYHLKYGADKARNELARLYDVVTTNETSFFRNPPQLDAFKIIMQKSYMNGAGPS
                     FPIRIWSAACSTGEEAYTLAIMLMEMAEIHRVSIPFSIIATDISSKVLESARKAVFSQ
                     YSVRNTDEAIKRKYFTEENNMYKLKESVKKNVKIDFMNLMDDDAYRLYRQMDFIFCRN
                     VLIYFDEKMKKKVVDHMYECLKPKGFLTIGHAESLHNISRAFKPLVFPGTIAYQKG"
     gene            2059..2442
                     /locus_tag="PKN85_02105"
     CDS             2059..2442
                     /locus_tag="PKN85_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43222.1"
                     /translation="MKTILIVDDSATIRKLLAYILKRKNYIIAEAEDGMDAMEKLSHV
                     QVDLVIVDLNMPNMDGIEFVKNLRDNYYYMDTPVIMLTTTKDDKLKKDALDAGVNMFL
                     NKPVQPNFLLYKVESLLQGEEEYNG"
     gene            2435..4279
                     /locus_tag="PKN85_02110"
     CDS             2435..4279
                     /locus_tag="PKN85_02110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNT43223.1"
                     /translation="MDDQALQRDEMQEIIDEFIAESSELMDNVIQDIVVIEQSQDEEV
                     VNSIFRAVHTIKGTSSFLGFNALSQLAHKAEDVLGVIRKGEMATDQTVADILLESFDL
                     MKLMIEDIRDQGSENQDASAVIVKLTDLLDPAKKAAEPQPEETKKIGEILVEEKIISE
                     SELEDVLRKQETEKDKKVGEIAVEEKYITETQLNKALVKQKAPKMEEQSIRIDVKKLD
                     ELMNLVGELVLGKNRLILVNSMARKGEESEAVFDNLTDITNYIEVITNELQLSVMRAR
                     LVPVSKVFNKIPRMVRDLCSEFKKDIELKVEGEETELDRSLIEALTDPLIHIIRNSVD
                     HGIELPEERAAKGKRAKGLLSIRAYNEGNHVIIEVYDDGKGINIQAVKDKVKEKGLMN
                     EAELASLSPKEAMNLIFIPGLSTAQKISKVSGRGVGMDVVRTNIEKMNGQAHIDSEEG
                     KWTKLTIKLPLTLAIMRALIVKVAEELFAIPLNTVTELVKLKEGLIKTVDKNEVLVLR
                     NTVIPIVDLSKAFLMQGAEDGSGYVVICSLGEKTIGIKVNTVIGQEEVVIKPLGEFLK
                     DIKGISGATIRGDGKVILILDIPSVILNYTMHRRATAQAQAQQIQMRN"
     gene            complement(4398..5675)
                     /locus_tag="PKN85_02115"
     CDS             complement(4398..5675)
                     /locus_tag="PKN85_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNT43224.1"
                     /translation="MKSNPVGSGATRPQEEPGEDTRVHIGRQPILNRARKIFGYELLF
                     RSGRANTANVIDNVHATASVMLNALNNIGISRLIGDKIGFINVDDRVLDSGIVELLPS
                     KMTVLELLETVEVDERVLALCAQARRNGYRFALDDFVTYDGAPEDMLHIASYVKVDLM
                     GTDRKDLPELVRKLKRHNLKLLAEKVETQEDFEVCRDLGFDYFQGYFFEKPSIISARS
                     ISPTQLVLLELSRILAREEEFFVIESLFRKNPELHIKLLQFINSAAFYTINKINSIGQ
                     AIALLGYRKLQKWVTLLLFAGEGPDARSAPLFERAVIRGRIMELLASRITRDTSAADM
                     AFITGVLSLVDALFQAPLEIILTDFNLSEEINGALLHRDGVLGKLVSVIETLEQENYD
                     EMETHLRDVNMTSTDLYIIENNAIIEYESMSGM"
     gene            5966..>6152
                     /locus_tag="PKN85_02120"
     CDS             5966..>6152
                     /locus_tag="PKN85_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT43225.1"
                     /translation="MNEGTILQLVTFKLGTEEFGVDILKVQEINKMMSITKIPNAPAF
                     IEGVINLRGKIIPIVDLR"
BASE COUNT         1494 a         1622 c         1810 g         1226 t
ORIGIN      
        1 ctgaagccgg cgggctacat cggcacgccg tcctttctca agatcatcct cgagaagggt
       61 gatgagggga cctttgggag gctcaaggag aagatcgggg acgagctcta cgagcatctt
      121 ctcggaagcg ccgccgagaa ccggcaacgt cccgtcgcgc tcgtgcgcat gctgacccat
      181 ttcaggatgc ctgaaacatg cggcgccatt ctcgatatcc tcaaggagat ggaccccgac
      241 gacgagaact atgaaatggt gctcacgctt tttgcttccc tgagcgaggt gtgggcggcg
      301 gatgccggcg gatacatgga gcgtgacgag gcgaaccttc tgccccttgt cagggcgtgc
      361 aagatgacgg gtgtgaaggt cggggaggct gccctcctga ggcacttcct gacagcaccc
      421 gtggaagtga agcgcgagat cgtgatgagc gttccggcgg tcgtagatgg aagcggatgc
      481 tccatcatca aggaggccct caaagacacc gacggccata tcaagggttt tgccgtcgac
      541 gcggtgggta ctctcaagct cacgaatctc aaggaagaca tcgtccggat cctcagggag
      601 gattttcacg atgtgagggt caaggcgttg aagaccctca tcaggctcga tcccaccctg
      661 gccgtggaga tgatcgccgc cttcgtggac aggggatcga cggatgacaa gaaggtgtat
      721 ctcgccgcga caggcctcat cgacggacag aagaacctgc ccttcatcac ccggctcctt
      781 ggcgatgagg acgagggtgt acggagaagc accatcgccg ttctcgggaa cttcgtcgac
      841 gacgagaact acatgggtct ccttcacgat gaactgatgg gtgacgagat accccatgag
      901 gtgctcaagg tggtcaagga caggagactc acccgtttca aggacaggct tgtcgggatc
      961 ttcgcggacg agggcaaggg catgtggaca cgatactacg cgctctctgc tctcggtacc
     1021 ttcgaggacc cggccctctt cgacatcttc gtgaggggtc tcggggatga caacgggctc
     1081 atcacgatag ggtgcatccg ggcgcttgcc gacctcaacg atgagcgcgc cgtggaccac
     1141 atacggccat acctcgtgag cggcaacgac gatatcaggt ccgccgcgga gatggttgtc
     1201 aacagaacgc agaatgcata ggaaccggcc atgacgaaag aagaatttac cgtcctgcga
     1261 gatttcatct atgagaaaac gggtatttac ttcaccgaaa cgaagaccta tctcctcgag
     1321 agcaggttga cgaaccggtt gaacgagctg ggtctcggtt ccttcgagga ctactactat
     1381 catctgaagt acggtgccga caaggcgagg aacgagctcg ccagacttta cgacgttgtc
     1441 accacgaacg aaacgagttt cttccgcaat cctccgcagc tcgacgcctt caagatcatc
     1501 atgcagaaat cctacatgaa cggcgcgggc ccgagctttc ccatccgcat atggagcgcg
     1561 gcctgttcca cgggcgagga ggcttacacg ctggccatca tgctcatgga gatggcggag
     1621 atccaccggg tcagcattcc cttcagcatc atcgccacgg atatttcgag caaggtcctc
     1681 gagtccgcgc ggaaggccgt cttcagccag tacagcgtcc gcaacaccga tgaggccatc
     1741 aagagaaagt attttacaga agagaacaat atgtataagc tgaaagaaag cgtaaagaag
     1801 aacgttaaga tcgacttcat gaacctcatg gatgacgatg cttacagact gtaccggcag
     1861 atggatttca tcttctgcag gaatgtcctc atctattttg acgaaaagat gaagaagaag
     1921 gttgtcgacc acatgtacga atgcctgaag cctaagggat tccttaccat cggtcacgcg
     1981 gaatcgctcc ataacatatc ccgggccttc aagccgctgg tattcccggg caccatcgca
     2041 tatcagaaag gatgatatat gaagacgata ctcatcgtgg atgactcggc gacgatcagg
     2101 aagctcctgg catacatcct gaaacggaag aattatatca tcgcagaggc cgaggacgga
     2161 atggacgcca tggaaaaact gagccacgtc caggtggacc tcgttatcgt ggacctcaac
     2221 atgcccaaca tggacggcat cgagttcgtg aagaacctgc gggacaatta ctattacatg
     2281 gacacgcctg tcatcatgct cacgacgacg aaggatgaca agctcaaaaa ggacgctctt
     2341 gacgccggcg tcaacatgtt cctcaacaag ccggtgcaac caaatttctt actgtacaag
     2401 gtagaaagcc ttttgcaggg ggaggaggag tacaatggat gatcaagctc tccagcggga
     2461 tgagatgcag gaaatcatcg atgagttcat agcggagtcg agtgaactca tggacaatgt
     2521 cattcaggac attgtcgtca tcgagcagag ccaggacgag gaggtcgtga acagcatttt
     2581 ccgggccgtt cacaccatca agggaacgtc gagctttctg gggttcaacg ccctgtccca
     2641 gctcgcccac aaggcggagg acgtcctcgg ggtcatccgc aagggggaga tggccacgga
     2701 ccagaccgtc gccgacatcc ttctcgagtc cttcgacctc atgaagttga tgatagagga
     2761 tatccgggac cagggaagcg agaaccagga cgcgtccgcc gtcatcgtta agctcacgga
     2821 ccttctcgat cccgcgaaga aggccgccga gcctcagccc gaggagacga agaagatcgg
     2881 cgagatcctc gtggaggaga agatcatctc ggaatccgag cttgaggatg tcctcaggaa
     2941 acaggagacg gaaaaagaca agaaggtcgg cgagatcgcc gtcgaggaaa agtatatcac
     3001 ggagacccag ctcaacaagg cactggtgaa acagaaggcc cccaagatgg aggagcagtc
     3061 gatcaggatc gacgtcaaga agctcgacga gctgatgaac ctcgtcggtg agctcgtcct
     3121 cgggaaaaac aggctcatcc tcgtcaacag catggcgagg aagggtgagg agagcgaggc
     3181 cgtcttcgac aatctcacgg atataacgaa ctacatagag gtcatcacca acgagctcca
     3241 gctttcagtc atgagggcga gactggtgcc cgtcagcaag gtcttcaaca agatccccag
     3301 gatggtgagg gacctttgct cggagttcaa gaaggacatc gagctcaagg tcgaaggcga
     3361 ggagacggag cttgaccggt ctctcattga agcgctgacg gaccctctca tccacatcat
     3421 acggaattcc gtcgaccacg gcatcgaact gccagaagag cgcgcggcga agggaaagcg
     3481 ggccaagggt cttctatcca taagggctta caacgagggc aatcacgtca tcatcgaggt
     3541 ttacgacgac gggaagggca tcaacatcca ggcggtgaag gacaaggtga aggagaaggg
     3601 gctcatgaac gaggcggagc tggcaagcct gtcccctaaa gaggcgatga acctcatctt
     3661 catcccgggt ctgagcacgg ctcagaagat aagcaaggtc tccggccgcg gcgtcggaat
     3721 ggatgtcgtc aggacgaaca tcgagaagat gaacgggcag gcgcacatcg attccgagga
     3781 aggcaagtgg acgaagctca ccatcaagct ccccctgacc cttgccatca tgcgggccct
     3841 tatcgtgaaa gtggcggagg agctctttgc catacccctc aataccgtga ccgaactcgt
     3901 caagctcaag gaagggctca tcaagacggt ggacaagaac gaggtcctcg ttctgcgcaa
     3961 caccgtcatc cccatcgtgg acctcagcaa ggcctttctc atgcagggcg cggaggacgg
     4021 cagcgggtac gtggtgatct gctctctcgg cgagaagacg ataggcatca aggtcaacac
     4081 cgtcatcggc caggaagagg tggtcatcaa gcccctcgga gagttcctca aggacatcaa
     4141 ggggataagc ggcgccacca tacggggcga cggcaaggtg atcctcatac tcgacatccc
     4201 ctcggtcatc ctcaactaca cgatgcacag aagggccacc gcccaggccc aggcgcagca
     4261 gatacagatg aggaactgaa agacggctta aacggttgaa cgttatgaac ccctgatagc
     4321 cctgtcgggc atcaggggtg ttcttttcct aaccctcaag cgattcaagc cattcaagcg
     4381 attcaagcgg tctttttcta cattccgctc atcgattcgt attcgattat cgcgttgttc
     4441 tcgatgatgt agaggtctgt ggaggtcatg ttgacgtccc tgaggtgggt ttccatctcg
     4501 tcgtagttct cctgttccag ggtctctatc acagagacga gtttgccgag gacgccgtcg
     4561 cggtgaagga gggccccgtt tatctcctcg gagaggttga agtcggtcag gatgatctcc
     4621 aacggagcct gaaagagggc gtccacgagg gacaggacgc cggtgatgaa cgccatgtcg
     4681 gcggcggagg tgtcgcgggt tatccgggag gcgaggagtt ccatgatcct gccccggatc
     4741 acagcccgct cgaagagggg ggccgaccgt gcgtcgggtc cttcgccggc gaagaggagc
     4801 agggttaccc atttttgaag cttgcgatat cccagcaggg ctatcgcctg acctatggaa
     4861 tttatcttgt ttatcgtgta gaatgccgcc gaatttatga actgaagcag cttgatgtga
     4921 agttcggggt tcttcctgaa gaggctttct atgacgaaga actcctcttc cctggcgagg
     4981 atccttgaaa gctcgagaag caccagttgt gtcggtgaaa tggacctcgc cgatattatc
     5041 gaaggctttt cgaagaagta gccctggaag tagtcaaaac cgaggtcgcg gcacacttcg
     5101 aaatcctcct gggtctccac cttctcggca agaagcttga ggttgtgcct cttcagtttt
     5161 ctaacgagct ccgggagatc tttcctgtcg gtgcccatga ggtccacctt cacgtaggag
     5221 gctatgtgga gcatgtcttc gggggcgccg tcataggtga cgaaatcgtc gagggcgaac
     5281 cgatagccgt tccttctcgc ctgggcgcaa agggccagga ccctttcgtc cacctccacc
     5341 gtctcgagga gctcgaggac ggtcatcttg ctcgggagga gctcgacgat gccgctgtcg
     5401 agcaccctgt catcgacgtt gatgaaccct atcttgtctc cgatgagccg cgagataccg
     5461 atgttgttga gggcgttcag cataacgctg gcggtggcgt ggacgttgtc gatgacgttg
     5521 gccgtgttgg ccctcccgct gcgaaagagg agttcgtagc cgaatatctt cctggccctg
     5581 ttcaggatgg gctgccggcc gatatggacc cgcgtgtcct cgcccggttc ttcctgtggt
     5641 ctcgttgcgc cgcttccgac tgggttactt ttcatcggtc ctggattccc tttacatctt
     5701 ttatcgactg aaacagagct aactaaagag gtcatcgacg ggcaaggaaa aaaagtacga
     5761 gggtgcaaga aagccgggcg tcgcgaatac ccgggccggt gttggaatgg tttcgggaag
     5821 ctgcgtcgct gaccctccga tctcacggct gattgtggtt gactgcgcct atcggttgta
     5881 gtatccatag gattgggtac acaatttgca gataatttaa gtcgggagag gaacttccga
     5941 ttatacagaa aagaggagga acctgatgaa tgagggaaca atactgcaac ttgtgacatt
     6001 caaactggga acggaggagt tcggggtcga tatcctgaag gtgcaggaga tcaacaagat
     6061 gatgagcatc accaagatcc ccaacgcgcc cgctttcata gagggcgtga taaatctgag
     6121 gggaaagatc attcccatcg tcgacctccg ca
//
LOCUS       DAOQTR010000052         6046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14437_length_6046_cov_4.117343, whole genome shotgun sequence.
ACCESSION   DAOQTR010000052 DAOQTR010000000
VERSION     DAOQTR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14437_length_6046_cov_4.117343"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKN85_02125"
     CDS             <1..204
                     /locus_tag="PKN85_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNT43226.1"
                     /translation="NNLSFRVWEEVTKCFNDTVFRTVIPRNVKLSESPSHGKPALLYD
                     ISSRGAESYLRLASEILEKRSEA"
     gene            201..1013
                     /locus_tag="PKN85_02130"
     CDS             201..1013
                     /locus_tag="PKN85_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNT43227.1"
                     /translation="MKKDPLGRGLSAILKDIEEKGTTRLIAIDQIVPNPAQPRIRMDE
                     RGIDELAASISEKGLLQPIILKRKGSVYEIIAGERRYRAAILAGLREVPAMVRDVDDR
                     EALEIALMENLQREDLGPLEVAAVYERFVEEFTYTHEDIAKKMGVDRSSVTNCLRLLK
                     LPAWVKELMSEGKLTQGHGRVILSLKGEREQKRFVEKVLHEGTSVRELERQARKASPT
                     KEPALAFLEETLVKALQTKVNITMRRNRGKIIIEFYSREDLERLSELITGLN"
     gene            1146..1571
                     /locus_tag="PKN85_02135"
     CDS             1146..1571
                     /locus_tag="PKN85_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNT43228.1"
                     /translation="MINFDATILYQFVHFLILLFILNFILFKPVLRALEKRQNAIKGL
                     TDGVEQAKQDTVDLEKNYESSFVEKRKPIIGGRDAVISEANKEAVHVIEKARSELSEE
                     LAKIKSEIEVEGRKVYDALRADVGKLSMEAAQKILKRSI"
     gene            1571..2065
                     /locus_tag="PKN85_02140"
     CDS             1571..2065
                     /locus_tag="PKN85_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNT43229.1"
                     /translation="MAPGGESVWSLIFKFFNFFVLVGLLIYFVGKPLKGFLRKRHQTV
                     KTQLEETQKALSEAQALRARYQEKMDKLEGEMEAFKKQTMQEAETEKKKIIDEAMGFA
                     ARIREQARLTAEQENKEVARRIKEEISRLTVEQAEKLITEKITQSDHDSLVEDFIVKL
                     RSMN"
     gene            2062..2604
                     /gene="atpH"
                     /locus_tag="PKN85_02145"
     CDS             2062..2604
                     /gene="atpH"
                     /locus_tag="PKN85_02145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNT43230.1"
                     /translation="MISQSIAKRYAKGLFNVGEKNGKYKEYQSEIEGILGVFDKEERL
                     KRAIVLPLVEVEKRREVLSDVLKSLQVSSPLASMFTMLLEKNRMGYLSLIRDVYNELV
                     DEKEGRVKGTLWTAFPMDDAAKGKIEKELGAKLGKEVLLTVQEDKSLIGGVKVAIKGT
                     IIDGSVKRQLHTLQENILKE"
     gene            2610..4118
                     /gene="atpA"
                     /locus_tag="PKN85_02150"
     CDS             2610..4118
                     /gene="atpA"
                     /locus_tag="PKN85_02150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNT43231.1"
                     /translation="MEIRADEISRIIEQKISGFEKEIDLQETGVIMTVGDGIARIYGL
                     ENAMAGELLELPHGITGMVQNLEEDNIGAVIFGEDYKIKEGDLAKRTGKIAQVPVGEA
                     LVGRVVDALGTPIDGKGPIDAKEFRPVEQIAPGVVVRQPVKEPLQTGIKEIDALIPIG
                     RGQRELIIGDRGTGKTVVALDTIINQKGKDVFCIYVAIGQKRFSVARIVDLLTEYGAM
                     EYTTVVAATASDSAPLQFLAPFAGTAMGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDIFYLHSRLLERSAKWDDAHGGGSLTSLPIIETQAGDVSAYIPTNV
                     ISITDGQIYLEPELFYAGVRPAINVGISVSRVGGNAQIKAMKQVAGRLRLEMAQYREM
                     AAFAKFGSDLDKATQALLARGSRLTELFKQGQYVPIAVEKQVVLLYAAANGFIDTYPE
                     SALKKYEAELTRFMDSKYTNILQEIGEKKAIDASIEEKLTKALNEFKEGFTY"
     gene            4131..4991
                     /gene="atpG"
                     /locus_tag="PKN85_02155"
     CDS             4131..4991
                     /gene="atpG"
                     /locus_tag="PKN85_02155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNT43232.1"
                     /translation="MATLRDIKRKIGSVNSTQTITRTMKMVSASKLRRAQTDLEKIKD
                     YAVKIEELTGRVVKNMPVDAHPLLETREEIKKVLIVSIGSDRGLCGGFNVNVALTAEN
                     FSKQNRDTYERVGVYVFGRKIRDYLAKRRIDVVKSWVDIKKVDQELVDAVAEEIIELY
                     LTGEFDKVYLSYTYFASPVKQEIVFEEFIPIKTPEDVEYIDYICEPDRMEIVGSLIPR
                     YISTKIYYALVESQTSEHAARMSAMENATNNCGEMVRYLTLVYNKRRQEGITNEMLDI
                     VGGAEALRGT"
     gene            5009..>6046
                     /gene="atpD"
                     /locus_tag="PKN85_02160"
     CDS             5009..>6046
                     /gene="atpD"
                     /locus_tag="PKN85_02160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNT43233.1"
                     /translation="MSSVEVRGKIVQVIGTVVDFRFPPDQLPPINGAIYVTNPAISDK
                     AENLILEVAQHVGDNTVRCIAMNTTDGLMRGQDAVYKGGQISIPVGKEILGRVLNVVG
                     DPVDNQGDVKTAMTYPIHRPAPSLTQQNTKIELLETGVKVIDLLEPYPKGGKVGLFGG
                     AGVGKTVVIMEMIHNIAQHHGGISVFGGVGERTREGNDLWLEMKGSGVLDKCALIYGQ
                     MTEVPGARARIGLTALTAAEYFRDEEGQDVLLFIDNIFRFTQANSEVSAQLGRMPSAV
                     GYQPTLATDLGELEERITSTLKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSR
                     QISELGIYPAVD"
BASE COUNT         1532 a         1581 c         1813 g         1120 t
ORIGIN      
        1 aacaaccttt ccttcagggt gtgggaagag gtcacgaagt gcttcaatga caccgttttc
       61 aggaccgtca tcccccgcaa cgtgaagctc agcgaatcac cgagccatgg aaaaccggca
      121 ctcctttatg atataagttc acgaggcgcc gagagctatc tccggctggc gtcggaaatt
      181 cttgaaaaga ggagcgaggc gtgaagaaag accccctggg aagaggactc tccgccatac
      241 tcaaggacat agaggagaag ggcacgacgc gactcatcgc gatcgaccag atcgtgccca
      301 accctgccca gccgcgcatc cggatggatg agagaggcat cgacgaactg gcggcatcca
      361 tcagcgaaaa aggccttctc cagcccatca tactcaagag gaagggaagc gtctacgaga
      421 tcattgccgg ggagaggcgc taccgggcgg ccattctggc gggcctgagg gaggtcccgg
      481 ccatggtccg ggatgtcgac gacagggagg cgctggagat cgccctcatg gagaacctcc
      541 agagagagga ccttggtccc ctggaggtgg ccgcggtgta cgagcggttc gtggaggaat
      601 tcacgtacac ccacgaggac atcgcgaaga agatgggcgt tgaccgcagc tccgttacga
      661 actgcctgcg tctcttgaag ctgcccgcat gggtgaagga actcatgtcg gagggcaagc
      721 tcacgcaggg gcacggacgg gtcatccttt cgcttaaggg tgaaagggaa cagaagaggt
      781 ttgtagaaaa ggtccttcat gaggggactt ccgtccggga actggagaga caggcaagga
      841 aggcgtcccc taccaaggag cccgcgctgg catttctgga ggagacgctg gtcaaggccc
      901 tgcagacgaa ggtgaacatc acaatgagaa ggaacagggg caagatcatt atagaattct
      961 attcgcggga ggatctggag cgcctgtctg agttgataac cggcttgaat taacggctgt
     1021 atcggggttg aagcacatca atcagcgttt cttgaaaata tgtgacaaaa ataattgaaa
     1081 tgccgacggg aattgtgtta aaaccctacg gatagttaaa cgccaatcta attaggaagt
     1141 tccctatgat taacttcgat gcgaccattt tgtaccagtt tgttcacttc cttatcctgc
     1201 tttttatcct gaacttcatc cttttcaaac cggtcctcag ggccctggaa aaacgccaga
     1261 acgccattaa agggttaacg gacggtgtcg aacaggcaaa gcaggacacg gtagacctgg
     1321 agaagaacta cgagtcctcc ttcgtggaaa agagaaagcc catcatcggc ggcagggatg
     1381 cggtcatctc ggaggccaac aaggaagccg tgcatgtgat cgagaaggcc aggagcgaac
     1441 tgtccgaaga gcttgcgaag ataaagagtg agatagaagt ggaaggcagg aaggtctatg
     1501 acgcgcttag agccgatgtc ggcaagcttt ccatggaagc ggcgcagaag atcttgaaaa
     1561 ggagtatctg atggcacccg gtggcgagtc ggtctggagt ctaatattca aattcttcaa
     1621 tttctttgtt ctggtcggcc tacttatcta ttttgtcggt aagcccctga agggtttcct
     1681 caggaagcgt caccagacgg tgaagacgca actcgaggaa acacagaagg ccttgagcga
     1741 ggcccaggcc ctgcgtgccc ggtaccagga gaagatggac aagctcgagg gcgagatgga
     1801 ggccttcaag aagcagacga tgcaggaagc ggaaacggaa aagaagaaga tcatcgacga
     1861 ggccatgggc ttcgctgcga gaatacggga acaagcccgc ctgacggcag agcaggagaa
     1921 caaagaggtg gcccggagga tcaaggaaga gatctcccgt ctcaccgtgg aacaggcgga
     1981 gaaactcatc accgaaaaga tcacccagtc cgaccatgac agtctggtgg aagatttcat
     2041 cgtaaaactg aggagcatga attgataagc caatccattg ccaagagata tgctaaaggt
     2101 ctgttcaacg tgggtgaaaa gaacgggaaa tacaaggaat accagtctga gatagaaggc
     2161 atccttggtg tcttcgacaa ggaagagcgc ctgaaaaggg ccatcgtgct gcccctcgtg
     2221 gaggtggaaa agcgccgtga ggtcttgagc gacgttctca agtcgcttca ggtctcctct
     2281 ccgctcgcca gcatgttcac catgctcctt gagaagaaca ggatggggta cctatcgttg
     2341 atcagggatg tctacaacga actcgtcgac gaaaaggaag gcagggtcaa gggcaccctt
     2401 tggacggcgt tccccatgga tgatgccgcg aaggggaaga tagagaagga actcggggcg
     2461 aaactcggga aagaggtcct gctgacggta caggaagaca aaagccttat cggcggggtc
     2521 aaggttgcaa ttaagggaac gataatagac ggaagcgtga aaagacagct gcacacgttg
     2581 caggaaaata tactgaagga gtagtgtcta tggagatcag ggccgacgaa ataagcagga
     2641 tcatagagca gaagatatcg ggtttcgaaa aggagatcga ccttcaggaa accggtgtca
     2701 tcatgacggt gggcgacggt atcgcaagga tatacgggct tgagaacgcc atggccggcg
     2761 agctccttga gctgccccac ggtattaccg gcatggtgca gaaccttgag gaagacaaca
     2821 tcggcgccgt tatcttcggc gaggactaca agataaaaga aggcgacctg gcaaaaagaa
     2881 cgggaaagat cgcccaggtc cccgtcggtg aggccctggt gggcagggtt gtcgacgccc
     2941 tcgggactcc gatcgacggc aaaggtccga tcgatgcgaa agagttccgg cccgtcgaac
     3001 agatagcgcc cggcgtcgtc gtgcgccagc ccgttaagga gcctctgcag acgggcatca
     3061 aggagatcga cgccctcatc cccatcggca ggggtcagag ggagctcatc atcggcgacc
     3121 gcggcacggg aaagaccgtc gtggccctcg atacgatcat caaccagaaa ggcaaggatg
     3181 tcttctgcat atacgtggcc ataggccaga agaggttctc cgtggcgagg atcgtcgacc
     3241 tgctgacgga gtacggcgcg atggagtaca ccacggtcgt cgcggcgacg gcaagcgact
     3301 ccgctccgct ccagttcctc gctcccttcg cgggcaccgc gatgggtgag tacttcaggg
     3361 acaacggcaa acatgccctc atcgtgtatg acgacctttc aaagcatgcc gtcgcgtacc
     3421 gccagctctc gctgctcctc aggagaccgc cgggacgtga ggcgtacccc ggggacatct
     3481 tctatctcca ctcacgcctt cttgaaagat cggcgaagtg ggatgacgcc catggcggcg
     3541 ggtcccttac ctccctcccg atcatcgaga cccaggcggg cgacgtttcc gcgtatattc
     3601 cgacgaacgt catttccatt accgacggtc agatctacct tgagccggaa ttgttctacg
     3661 ccggtgtcag gcccgcgatc aacgtcggta tctcggtatc gagggtcggc ggcaacgcac
     3721 agatcaaggc aatgaagcag gtggcaggca ggttgaggct ggaaatggcc cagtaccgcg
     3781 agatggcagc gtttgccaag ttcggttccg accttgacaa ggccacgcaa gccctccttg
     3841 cccgcggttc acggctgacg gagcttttca agcagggcca gtacgttccc atcgccgtcg
     3901 agaaacaggt cgttctcctc tacgccgcgg cgaacgggtt catcgatacc tatcccgaga
     3961 gcgctctcaa gaaatacgag gcggagctga cgcgcttcat ggattccaag tataccaata
     4021 tcctccagga gatcggggag aagaaggcca tcgacgcgtc gatcgaagag aagcttacga
     4081 aggcgctcaa cgagttcaag gaaggattta cctactaaag ggagctttca gtggcgacgc
     4141 taagagatat caagagaaag ataggaagtg tcaacagcac ccagacgatc acgaggacga
     4201 tgaaaatggt ttccgcctcg aaactgaggc gggcccagac ggacctcgag aagatcaagg
     4261 actacgcggt gaagatagaa gagctgaccg gacgtgtcgt caagaacatg cccgtcgatg
     4321 cccatcccct tctcgagacg agagaagaga tcaagaaggt cctcatcgtt tccatcggct
     4381 ccgacagggg cctgtgcggc gggttcaatg tcaacgtggc tctgacggcg gagaacttct
     4441 cgaagcagaa ccgcgacacc tatgaaagag tgggtgtcta cgttttcggc cggaagatcc
     4501 gggattatct cgccaagaga agaatagatg tggtgaagag ctgggtcgac atcaagaagg
     4561 tcgaccagga gcttgtggac gccgtcgccg aggagataat agaactgtac ctgacaggcg
     4621 agttcgacaa ggtgtacctc tcctacacgt atttcgcgtc cccggtgaag caggagatcg
     4681 tcttcgagga attcatcccc atcaagacgc ccgaggatgt cgagtatatc gattacatat
     4741 gcgagcccga ccgcatggag atcgtcgggt ccctcatccc ccgttacata agcacgaaga
     4801 tatactacgc cctggtggaa tcccagacgt cggagcacgc ggcccggatg agcgcgatgg
     4861 agaacgcgac gaataactgc ggtgagatgg tgcggtatct caccctcgtt tacaacaaga
     4921 gaagacagga aggtataacc aacgaaatgt tggatatcgt tggtggcgct gaagccttgc
     4981 gaggaacata atctaaggag gaagatagat gagcagcgtg gaggtcagag gaaagattgt
     5041 acaggtcatt ggaacggtcg tagatttcag gtttccacct gatcagcttc caccaataaa
     5101 cggcgcgatt tacgtaacca accccgctat cagcgacaag gcggagaacc tcatccttga
     5161 ggttgcccag catgtcggtg acaatacggt cagatgcatc gccatgaaca cgactgacgg
     5221 tctcatgcgc ggccaggatg ccgtctacaa gggcgggcag atatcgatcc ccgtcgggaa
     5281 ggagatcctg ggcagggtgc tcaatgtcgt cggcgatccc gtggacaacc agggagatgt
     5341 caagacggcc atgacgtacc cgatccaccg cccggcaccc tcgctgaccc agcagaatac
     5401 gaagatcgag cttctcgaaa cgggcgtcaa ggtcatcgac cttctcgagc cttatccgaa
     5461 gggcggcaag gtcggtctct ttggcggcgc cggtgtcggc aagacggtcg ttatcatgga
     5521 gatgatccac aacatcgctc agcaccacgg cggcatatcc gttttcggtg gtgtcggtga
     5581 gcgcaccagg gagggaaacg acctctggct cgagatgaag ggctccggtg tactcgacaa
     5641 gtgcgcgctc atttacggac agatgacgga agtccccggt gcccgggcga ggatcggtct
     5701 tacggccctc actgccgccg agtatttccg cgatgaggaa ggccaggacg tgctcctctt
     5761 catcgacaac atcttccgtt tcacccaggc caattccgag gtctcggcgc agctcggccg
     5821 tatgccctcc gccgtcggtt accagccgac cctggcgaca gaccttggag agcttgagga
     5881 gcgcatcacc tccactctga aaggttccat cacctccgtt caggccattt acgtccctgc
     5941 cgatgacctc acagacccgg cgccggcaac gacgttcgcg catctcgacg ccacaacggt
     6001 tctttcccgt cagatctcgg agcttggtat atacccggct gttgac
//
LOCUS       DAOQTR010000053         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14611_length_5992_cov_3.840660, whole genome shotgun sequence.
ACCESSION   DAOQTR010000053 DAOQTR010000000
VERSION     DAOQTR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14611_length_5992_cov_3.840660"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKN85_02165"
     CDS             <1..442
                     /locus_tag="PKN85_02165"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HNT43234.1"
                     /translation="FCSACGKSIGAPQGEVNQGRTSQPPLSEPPTRPLTSNIALDVIL
                     SVITCGIYWFFWQARQMRAINHLLGQERYSFWLWFFLTLITCGLYNIYYEYYMAQGIV
                     EAQDSRGYLRSKDLPVLSLILTIIGLNIVTDAIQQNEINKFFGK"
     gene            444..848
                     /locus_tag="PKN85_02170"
     CDS             444..848
                     /locus_tag="PKN85_02170"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNT43235.1"
                     /translation="MDHHLIPRKMASAFALLLLVLAVVHATGILPFGTLAGILPVFCP
                     FELLTGIPCPGCGMTRAILCLIAGNPSDALLYNPFSFFLLTLVTASILPRRWLERIPT
                     GIRSVLPRAYAVILGLVITFWIFDRLLPALTA"
     gene            complement(1051..1782)
                     /locus_tag="PKN85_02175"
     CDS             complement(1051..1782)
                     /locus_tag="PKN85_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43236.1"
                     /translation="MNIRGPFYFITKKTGKAIWDYRMLSDGDKVIIAVSGGKDSLCLL
                     KIMQERLKFVPINYELIACHVDMGFPWVNADMLADYFEKEGLSYIIASAPEDWTGDIE
                     NLDCFWCSWNRRKAVFDVAADIGANKIAFAHHMDDIIETMLLNLFFQGEIGTMMPYQE
                     MFDGELAIIRPLAYVEEKELARLATILNLPVIASECPRGDVSKRSLVKGIVADLKKHN
                     RNVKKNIFRSLAKIRQEYLPEKAPG"
     gene            complement(1779..2324)
                     /locus_tag="PKN85_02180"
     CDS             complement(1779..2324)
                     /locus_tag="PKN85_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT43237.1"
                     /translation="MKNAVILFGSPRKKGNTVELARAVSATLKERGWGVRMLYLNDLN
                     IRPCQGCYVCLPGGVCKINDDMKDVRKYIMESDLIVYATPIYWFGPSGQLKLVMDRSI
                     AFMDENYGSRIEGKKAITLTTFADKDMDSCRPAIDIFQKTFDLLKVAYAGQVNAPGCE
                     PEGGVRQEYIDKARALAESLA"
     gene            2767..3162
                     /locus_tag="PKN85_02185"
     CDS             2767..3162
                     /locus_tag="PKN85_02185"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HNT43238.1"
                     /translation="MSNIFLGIITLAAVVLVVYVVYMILNLKRTIASLERFVETTEAT
                     LQPALEELQATLKSVRKITDDVGTLTDDIRHISGSVRNVGEAVDQVSYAVKSAAQASV
                     SETKGLKAGIRAGVSYFLRNACLRGGTQQ"
     gene            3179..3481
                     /locus_tag="PKN85_02190"
     CDS             3179..3481
                     /locus_tag="PKN85_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNT43239.1"
                     /translation="MGQQDSGFSAGTVLLSFFIGGILGAGVALLTAPKTGEETRKMIK
                     EFAEDARDRAGEYAGQVKEKASGFVDKGKDFIDKEKNIITKAVEAGKEAYEREKQG"
     gene            3562..4632
                     /locus_tag="PKN85_02195"
     CDS             3562..4632
                     /locus_tag="PKN85_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT43240.1"
                     /translation="MITGNRFYNLVLIFIVAALGYLTYRILDPFLSPIMWAIVFSVVF
                     YPFYAFVLRYVKYRAAASVLTVFAIFLIIIGPFSYFAYLLTQETVALVNRIQASEGNI
                     LQNFLSHPVVSAFVQKLLVVLRMTEAEFYEALTQGILKVAKESTGLIKIGFGNIVAGG
                     INFIFMFLCTFFFLEDGPALIEKLEGFMPFSRRQRTRLLQQTKDIVVSTIYGGVVVAM
                     TQAIIGGVTFALLGIHSPMVWGLAMFVSSFIPVVGTFIVWGPMVIYLFFQAQYLKGVI
                     LILVGVLAISSVDNILRPLIIKGKMKMPTVAIFFAILGGIKFFGFIGFILGPLVLALF
                     ISVFDIFRYSEEERYKADDAEQ"
     gene            complement(4762..5670)
                     /locus_tag="PKN85_02200"
     CDS             complement(4762..5670)
                     /locus_tag="PKN85_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT43241.1"
                     /translation="MGENDLQRLLAYLAGAAKDVSRGEYRSAAEIFELTKSGKYPPDI
                     TELAEAFGMMMVMVEAREERLERLIGDLQEQKLVLENTSRALHQANIGVLEVLGSAIA
                     KRDHGTSAHNYRVTLYALQIAAALGHAEEHVRSLVKGSFLHDVGKIGISDTILLKEGS
                     LTGGEFETMKGHVRHGSDIIASYAWLGDCVDVVLYHHEKFDGSGYLSGLREGEIPLNA
                     RIFAVADVFDALTSARPYKTALSYEEAMDMMRKARGSHFDPEVVDGLCRVGDVLYREV
                     YFADEDRLRHTLKQHIHELFKEDSDV"
BASE COUNT         1250 a         1750 c         1610 g         1382 t
ORIGIN      
        1 gttctgctcc gcctgcggca aatccatagg cgccccgcag ggggaggtga accagggaag
       61 gacgtcgcag ccccctctct ccgaaccccc cacgcgccct ctcacctcga acatcgcact
      121 cgatgtcatc ctttccgtca taacctgcgg gatctactgg ttcttctggc aggcccgcca
      181 gatgcgggcc atcaaccacc tcctcggcca ggagagatac agcttctggc tctggttctt
      241 cctgacgctc atcacatgcg gtctctacaa catctactac gagtactaca tggcccaggg
      301 catcgtggag gcccaggatt cacgcggcta cttgaggtcc aaggacctgc cggtcttaag
      361 cctcatcctt acgataatcg gcctcaacat cgtgaccgac gcgatacagc agaacgagat
      421 caacaagttt ttcggcaaat aagatggacc accaccttat cccccggaag atggcctcag
      481 cctttgccct tcttcttctt gtcctcgccg ttgtgcacgc gacgggtatc ctgcccttcg
      541 gcacgctcgc ggggatcctt cccgtcttct gtccctttga gcttctcacg ggcatcccct
      601 gccccggctg cggcatgacc cgcgccatcc tctgcctcat cgctggcaac ccctccgacg
      661 ccctcctcta caaccccttt tccttcttcc tcctcaccct cgtcacggca agcatcctgc
      721 cgcgccgctg gctggaaagg ataccgacag gcatccggtc cgtcctgccg cgcgcctacg
      781 cggtcatcct cggcctcgtc atcaccttct ggatattcga caggctgctg ccggccttga
      841 ccgcctaaag gctgcttaaa ccgcaactca ttttcttcgc cggaataacg aaagagcccc
      901 aaatcgactt cagaaatgac tttgtcaacg gcctcttgaa cgttaaaggc caaaaagggt
      961 ccttcatccg gggtctatag ggacggtatg agggagaatt gtctctaaac gttcaagctg
     1021 ttcaagcggc tcgagcggct gcaactgtcc ttatcctggt gctttttccg gcaggtattc
     1081 ctggcggatc ttggcgaggc ttctgaagat gttcttcttc acgttgcggt tgtgtttctt
     1141 gagatcggcg actatgcctt tcaccaggct gcgtttcgat acgtcgcccc ggggacactc
     1201 cgaggcgatg acgggcaggt taagtattgt ggcgagacgg gctagctcct tctcttcaac
     1261 gtaggcgagc gggcggatga tggcaagctc accgtcgaac atctcctggt atggcatcat
     1321 ggtaccgatc tcgccctgga agaagagatt gaggagcatc gtttcgatga tgtcgtccat
     1381 gtggtgggcg aaggcgatct tgttggcccc tatgtccgcc gcgacgtcga agaccgcttt
     1441 gcgtctgttc cagctgcacc agaaacagtc gaggttctcg atgtcccccg tccagtcctc
     1501 gggggcgctg gcgatgatgt aggagaggcc ttccttttcg aagtagtcag cgagcatgtc
     1561 cgcgttgacc caggggaagc ccatgtccac gtgacaggcg atgagttcgt agttgatggg
     1621 cacgaacttg agccgctcct gcatgatctt gagaaggcac aggctgtcct tgcctccgga
     1681 aacggcaatg atgaccttgt ccccgtcgga gagcattctg taatcccaga tggccttgcc
     1741 cgtttttttg gtgataaagt agaaggggcc gcggatgttc atgccagcga ttcggcgagg
     1801 gcccgtgcct tgtctatgta ctcttgcctg acccctccct ccggctcgca gcccggcgcg
     1861 ttgacctgtc ccgcatatgc caccttgaga aggtcgaagg tcttctggaa gatgtcgatc
     1921 gccggacggc aggaatccat gtccttgtca gcgaaggtcg tcagggtgat ggcctttttc
     1981 ccctcgatac gggaaccgta gttctcgtcc atgaaggcga tggagcgatc catgacgagc
     2041 ttgagctgcc ccgaggggcc gaaccagtat atgggggtcg cgtaaacgat gaggtccgat
     2101 tccatgatgt acttgcggac gtccttcata tcgtcattga tcttgcagac gccccccggc
     2161 aggcagacat agcagccctg gcagggccgg atgttgaggt cgttgagata gagcatcctt
     2221 acgccccacc ctctctcctt caacgtggcc gacaccgcac gggccagttc gacagtattg
     2281 cctttcttgc ggggactccc gaaaaggata accgcattct tcatcacatc ctcctcagag
     2341 acaggtaatg ggtaatgagc cgtgggcgac agggacacgt gaccacaacc tgttttttgg
     2401 ttgccttatt atatggacag acggtttcaa ctttcaagaa aagaagaccg tttcaggttt
     2461 caggtttcga gctaaagaca agaaccggag aacagcggcc aaaaaggttc tgatcctccc
     2521 ggacagtcag actgtgaaac ctgtctgagc ataagggaac tgtacctccc tggctcttaa
     2581 ttcgcaacgc gcaacccgga acccgcaact gtctttcaac tcaaacgtgg aacctgaaac
     2641 ggttttttct tcccgaaacc ttgtcttacg gtactacaga ggttataatg tattaagttc
     2701 gttcacggga tagggcataa gctacgtatc ctgctccgcg gtcgatatcg acaaagaggg
     2761 ggtgccatga gcaacatctt tctgggtatc ataaccctgg cggcagttgt tctcgtagtg
     2821 tacgtcgtct acatgatact aaacctcaag aggaccatag cctcgcttga aaggttcgtg
     2881 gaaaccacgg aggcgaccct ccagcctgca ctggaggaat tgcaggcaac cttgaagagc
     2941 gtcaggaaga tcacagacga cgtgggcacc ctgacggacg atatccggca catctcgggc
     3001 tcggtgagaa atgtcggaga ggcggtggac caggtgagct acgcggtaaa gagcgcggct
     3061 caggcgtcgg tcagcgaaac aaaagggttg aaagccggaa tcagggcagg cgtgagttac
     3121 tttctgagga atgcctgctt gaggggcggg acgcagcaat aaaaaaagga ggtagtgtat
     3181 gggacagcaa gacagcggtt tcagtgcggg cacggttctt ctctctttct tcatcggcgg
     3241 gatactaggc gcgggagtgg ccctcctcac ggcaccgaaa acgggagaag aaacgcggaa
     3301 gatgatcaag gagttcgcgg aggacgcgag agacagggcc ggggagtatg cgggccaggt
     3361 caaggagaag gcatccggct tcgtcgataa gggcaaggat tttatcgaca aggagaagaa
     3421 catcatcacc aaggcggtgg aggctggaaa ggaagcttac gaaagagaaa aacagggctg
     3481 aaataccatc gatactctga tatcattcac taaggctgtc cccgtgggag ggggcggcct
     3541 tatgcttctt cggtcgggcc aatgatcacc gggaacaggt tttacaatct cgtcctcatc
     3601 ttcatcgtcg cggcgctcgg gtatctgaca tataggatcc tcgacccctt tctgtccccc
     3661 atcatgtggg ctatcgtctt ctccgtcgtc ttctacccct tctacgcgtt tgtcctcagg
     3721 tacgtaaaat accgggcggc cgcttcggtg ctgacggtct ttgccatttt cctgatcatc
     3781 atcgggcctt tctcctattt cgcctatctc ctgacccagg agacggtcgc cctcgtgaac
     3841 cgcatccagg caagcgaggg caacatcctc cagaactttc tcagccaccc ggtggtcagc
     3901 gccttcgtcc agaagctcct cgtcgttctt cgcatgacgg aagcggaatt ctacgaggcc
     3961 ctcacccagg gcattctgaa ggtggccaag gagtcaacgg gtctgatcaa gataggtttc
     4021 ggcaatattg tcgccggcgg catcaacttc atcttcatgt tcctgtgcac cttcttcttt
     4081 ctcgaggacg gtcccgccct cattgaaaaa ctggaaggct tcatgccctt ttccaggcga
     4141 cagcgcacac ggctcctcca gcagacaaag gacatcgtcg tctccaccat atatggaggc
     4201 gtcgtcgtcg ccatgacaca ggcgatcatc ggcggcgtca ccttcgccct tctcggcatc
     4261 cactccccca tggtctgggg ccttgccatg ttcgtctcat ccttcattcc cgtggtgggg
     4321 accttcatcg tctggggccc catggtcatc tatcttttct ttcaggcaca gtatcttaag
     4381 ggcgtcatcc tcattctcgt cggtgtcctc gcgataagct ccgtggacaa catcctgagg
     4441 ccgctaatca tcaagggcaa gatgaagatg cccacggtcg ccatattctt cgccattctc
     4501 gggggcatca agttcttcgg tttcatcggt ttcatcctgg gtccgctcgt gctcgccctt
     4561 tttatctccg tcttcgacat cttccggtac tccgaagagg agagatacaa ggcagacgac
     4621 gcggaacaat gatcaggttc gaatagctgg aaaaaaagga aatgaccaaa ccttaaaggg
     4681 gtgaacggaa aagcggggac cggcgatcgt gaaccagatc gcggcttcct gccggaaagg
     4741 atcttccaac tgacagatcc gtcacacgtc gctatcttct ttgaaaagct cgtgtatgtg
     4801 ttgcttcaag gtgtgccgca gccggtcctc gtccgcgaag tatacctccc ggtagaggac
     4861 atccccgacc ctgcacagac cgtcgacgac ctcggggtcg aaatgcgacc ccctcgcctt
     4921 ccgcatcatg tccattgcct cttcgtagga cagggcagtc ttgtacggtc gggcgctggt
     4981 cagcgcgtcg aacacatccg cgacggcaaa gatccgcgca ttgagcggga tctcgccttc
     5041 cctgagcccc gacaggtacc cgctcccgtc gaacttctca tgatgataga ggacgacgtc
     5101 gacgcagtca cccagccagg catagctggc gatgatatcg gagccgtgcc gcacgtggcc
     5161 cttcatggtc tcgaattccc cgcccgtcag gcttccttcc ttgagaagga tggtgtcact
     5221 gatgcctatc ttgcccacat cgtggagaaa agacccctta acgaggcttc tcacatgttc
     5281 ttccgcgtgg cccagggcgg cagctatctg caatgcatac agggtgacgc ggtagttgtg
     5341 ggcgcttgta ccgtggtccc ttttcgcgat ggcgcttccc aggacctcga gaactcctat
     5401 gttggcctgg tgcagggccc gcgaggtgtt ctccaggacc aacttctgct cctgaaggtc
     5461 ccctatcagc ctttcgagcc gctcttcacg ggcctcgacc atcaccatca tcatgccgaa
     5521 ggcttcggca agctccgtta tatccggggg gtacttcccg gatttcgtga gctcgaatat
     5581 ctccgcggcg gagcgatact cccctcgtga cacgtctttc gccgccccgg ccagatacgc
     5641 aaggagcctc tgcaggtcat tttcgcccaa acaaaccccc gtggacccgg cgcgtgtttc
     5701 cttatctttg tgccgaacat ccacgctcat tatcggacat gtggggcaag gtttcaagtt
     5761 ccacgtttca ggtttcaggt agaaaagccg ctttcaagtt tcaggaagaa aaaaaccgtt
     5821 tcaagtttca gattccaggt ttcaggttaa agacgagaac cggaagacca tggctcttca
     5881 actttcgagc ctatagttga agtacagtgg ccttccggta tccgtttttt ggtctttaac
     5941 cttgtatgtt actccccatg gaatatcaca ggccgtccat gataagaaga at
//
LOCUS       DAOQTR010000054         5970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14672_length_5970_cov_3.980558, whole genome shotgun sequence.
ACCESSION   DAOQTR010000054 DAOQTR010000000
VERSION     DAOQTR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14672_length_5970_cov_3.980558"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PKN85_02205"
     CDS             complement(<1..954)
                     /locus_tag="PKN85_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43242.1"
                     /translation="MIRLLMENAVQQIYVTNRGTAATARKVSGALHCEVSTNEKIAFE
                     LALAGAYVRKRTACILSTEGLYEALDPVMSSAYTGVAGSFVVVAVQENDEEVTPLGPF
                     SKLPLIVTEKEEELERAVRYGLELSEEYRIPVIVQAVPAGCGTGRKTAGQTAKTAGNA
                     AMLVKEPGRWAATPKFRFQLHGELNEKIRKIGEAFETYEDNLIISKGSTGLVTDRLSR
                     LEFYDEDASVLRLATVFPLPRKLVQGFVDSMDEVLVVETYPAIELQIPDRSKVKRGFV
                     DTGRGRPQYDEIIAGFSVIRDRLGPASSINMAHGIKRLHPER"
     gene            complement(983..2083)
                     /locus_tag="PKN85_02210"
     CDS             complement(983..2083)
                     /locus_tag="PKN85_02210"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT43243.1"
                     /translation="MLLSDRVMQMRPSGVRKIFDLAQKVKNPINLSIGEPDFDIPDVV
                     KDEGIKWIQKGFNKYTPSGGIPELREKVLGHLRGKGIVCEDAIITAGVTGGLLLALMT
                     TLNPGDEVIIPDPYFVLYEYQVLLLGGRPVFFDTYPDFTIREEELRRAITDRTRVILI
                     NSPNNPTGMVNSREELEMVARVAREKDLLVFSDDIYDRFVFDEEEGEKPYLGKMYEKT
                     LTFGGFSKTWGMTGWRLGYVAGPSEVIQCMITMQQYAFSSVTSFAQKAALVALDEGMD
                     DMIAGYRKKRDLIYNGLKDTYNVVKPKGAFFIFPEIPGGNAMAFVERALENNLFIIPG
                     SVFSARDTHVRISFAATEENLLKGIDVLRRMA"
     gene            complement(2084..2380)
                     /locus_tag="PKN85_02215"
     CDS             complement(2084..2380)
                     /locus_tag="PKN85_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43244.1"
                     /translation="MGAFIAFPITHDPSPITEVSMEIYCGQLGMIIGFDYCLQVQNGL
                     PCSNTFGCWKERVDVVALLRAKFTDEELRLAFNTLPRSRLDRILESARSACKEE"
     gene            complement(2396..3757)
                     /locus_tag="PKN85_02220"
     CDS             complement(2396..3757)
                     /locus_tag="PKN85_02220"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNT43245.1"
                     /translation="MDKKIFLNEKDMPRAWYNIQADLETPLPPPLNPETGEPVTPDML
                     EAIFPMNLIEQEVSTERFIDIPGEVMERLLMWRPTPLCRATGLEKFLGTPAHIYYKNE
                     GVSPPGSHKPNTAIAQAYYNKEAGIKRLTTETGAGQWGSALAFACNQFGIELKIYMVR
                     VSFNQKPYRRVMMETWGGKCVPSPSPDTKAGRAFLEGNPDHPGSLGMAISEAIEDAVT
                     SKNTKYSLGSVLNHVLLHQSVIGLEAQRQLELAGEYPDVVIGCAGGGSNFAGIAFPFI
                     RDKIHGKEIRVIAAEPTSCPTLTRGRYVYDFGDTAGMTPLLPMYSIGHGFVPAPIHAG
                     GLRYHGMAPLVSKVFADGLIEARAYNQIETFSAGVIFARTEGFIPAPETNHAIACAVD
                     EAKRAKEEGKEKVILMNFSGHGVIDLPSYDAFLSGKLQDYAMPDNEITELLRSLEKYP
                     KPK"
     gene            4216..4860
                     /gene="fsa"
                     /locus_tag="PKN85_02225"
     CDS             4216..4860
                     /gene="fsa"
                     /locus_tag="PKN85_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNT43246.1"
                     /translation="MKFFIDTANINEIRKGMEMGLVDGVTTNPSLLAKEKKDPDAVLR
                     EILQTVPGPVSLEVISTTAEGMCEEARKLAALGENAVVKIPMTGEGIRAVRTLTDEGI
                     KTNVTLIFQPVQALIAAKAGATYVSPFIGRLDDISQRGMDIIENIVTIFYNYAFETEV
                     IVASIRNPTHVLDAALVGADIATIPFNVLSQLMNHPLTDIGLQKFLKDWEGIKK"
     gene            complement(4850..>5970)
                     /locus_tag="PKN85_02230"
     CDS             complement(4850..>5970)
                     /locus_tag="PKN85_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43247.1"
                     /translation="QKMEVIGQLAGGIAHDFNNILSAIIGYSNLVQIKLPPDDPVRKY
                     VDQIVASSGKAAILTQSLLAFSRKQIIDPKPMDVNGAITGTKKLLSRLITEDIVLRTE
                     LCPGFLIVMADLVQIDQVLMNLVANARDAMPTGGILTISTKRVNGTDGERGNRFACIS
                     VSDTGVGMDARTKEKVFEPFFTTKESGKGTGLGLAIVYGIIQQHNGSISVASELGHGT
                     TFEVFLPIVEAVPDKEAQTVADDPRGSEKILVVEDNEELRNLLRTVLSEQGYRVFEAV
                     DGLDAIEAQHRCRADLIILDVVMPKMNGKEAYDRLKSMDPDIRVLFMSGYTDDIIHQK
                     GILDATIEYIAKPILPRDLLKKVRELLDEETLKKAPIS"
BASE COUNT         1168 a         1790 c         1642 g         1370 t
ORIGIN      
        1 tctctcggga tggagcctct tgatgccgtg cgccatgttt atcgaagagg cggggcccag
       61 ccggtccctg attacggaga accctgctat gatctcatcg tattgcggtc tgccgcgtcc
      121 ggtatcgacg aacccccgct tcaccttcga tctgtccggt atctggagtt cgatcgccgg
      181 ataggtctcg acgacgagga cctcgtccat gctgtcgacg aaaccctgca cgagcttccg
      241 cggcagggga aagacagttg ccagcctgag cacgctcgcg tcctcgtcgt agaactcgag
      301 acgggacagc cggtccgtga caagtcccgt tgaacccttt gagatgatga ggttgtcctc
      361 ataggtctcg aaggcctcgc cgatcttcct gatcttttcg ttgagctccc cgtgaagctg
      421 gaaacggaac ttcggcgtcg ccgcccacct gcccggctcc tttacgagca tggccgcgtt
      481 ccctgccgtc tttgcggtct gccccgcggt cttacggcct gtgccgcacc cggccgggac
      541 ggcctgcacg ataacgggga tcctgtactc ctcggaaagt tcgagcccgt acctgacggc
      601 cctttccagt tcttcttcct tttcggtgac gataaggggc agcttcgaga acgggccgag
      661 aggggtcacc tcctcgtcgt tctcctgaac ggccacgacg acgaaggacc cggccacccc
      721 cgtgtaggcg gagctcatga cggggtcgag ggcctcgtag agaccttccg tggagaggat
      781 gcaggccgtc ctcttgcgca cgtaggcgcc tgccagggca agctcgaagg ctatcttctc
      841 gttcgtcgaa acctcgcaat gcagcgcgcc cgacaccttc cgcgccgtcg ccgccgtgcc
      901 cctgttcgtc acgtagatct gctgcacggc attctccatc aacaacctga tcacattctc
      961 atgcatcgtt ccgaaaactc ctttacgcca ttcttctcag tacgtcgatt cccttgagga
     1021 ggttctcctc ggtggctgcg aaagagatgc gcacatgagt gtcccttgcc gaaaaaacac
     1081 tgcccggtat gatgaagaga ttgttctcca atgccctttc cacgaatgcc atggcgttgc
     1141 cgccgggaat ctcgggaaag atgaagaagg cccccttcgg cttaaccaca ttgtatgtgt
     1201 ccttgagccc gttgtaaatg aggtcccgtt tcttacggta cccggcgatc atgtcgtcca
     1261 tgccctcgtc gagggcaacg agggccgcct tttgcgcgaa ggacgtgacg ctcgagaagg
     1321 cgtattgctg catcgttatc atacactgaa tgacctcgct cggccccgcc acgtacccca
     1381 ggcgccaacc cgtcatcccc caggtcttcg agaagccgcc gaaggtgagc gtcttttcgt
     1441 acattttgcc caggtacggc ttctccccct cctcttcatc aaagacgaag cggtcgtaga
     1501 tatcgtcgga gaagacgagg aggtccttct cgcgcgccac cctcgccacc atttccagct
     1561 cttcccggga attgaccatg cccgtcggat tgttggggct gttgatgagg atgaccctcg
     1621 tcctgtccgt tattgcccgg cgcagttcct cctccctgat cgtgaaatcg ggataggtat
     1681 cgaagaagac gggccgccct cccagcagca ggacctggta ctcgtagaga acgaagtagg
     1741 ggtcggggat gatgacctcg tcaccggggt tgagcgtcgt catgagggcc agaagaagac
     1801 ctcccgtcac ccccgccgtg atgatggcgt cctcgcagac tatccccttt cccctgagat
     1861 gaccgaggac cttttcccga agctccggga tccctcccga gggcgtgtac ttgttgaaac
     1921 ccttctgtat ccactttatc ccttcatcct tcaccacatc gggaatatcg aaatcaggtt
     1981 ccccgatgct gaggttgatg gggttcttca ctttttgcgc caggtcgaag atctttcgca
     2041 cccccgatgg cctcatctgc atgaccctgt cggaaagcag catctattcc tccttgcaag
     2101 ctgaacgggc ggactccagt atcctgtcga gcctcgatct cggcagtgtg ttgaaggcca
     2161 atcgcaattc ttcgtcagta aactttgccc taagaagagc gaccacgtca accctttctt
     2221 tccagcaccc aaaggtgttg gagcagggca aaccgttctg gacctggaga caataatcga
     2281 acccgatgat catccccagc tgaccacaat agatctccat agaaacctcg gtgataggtg
     2341 atgggtcatg ggttatggga aaggcaatga atgcacccat cacccattac ccgcctcatt
     2401 taggtttcgg atacttctcc aggctgcgga ggagttccgt tatctcgttg tcgggcatcg
     2461 cgtaatcctg gagcttgccc gacaggaagg catcatagga cggcaggtct atgacaccgt
     2521 ggccgctgaa gttcatgagg atgaccttct ccttaccctc ttccttcgcc cttttcgcct
     2581 catcgacggc acaggctatg gcgtggttcg tctccggcgc cgggatgaac ccttccgtcc
     2641 gggcgaagat aacgcccgcc gagaacgtct cgatctggtt gtatgcgcgc gcctcgatga
     2701 gcccgtcggc gaagaccttg ctgacgaggg gtgccatgcc gtgataccga aggccccccg
     2761 cgtgtatcgg cgcgggaacg aatccgtgcc cgatggagta catgggcaga agcggcgtca
     2821 tcccggcggt gtccccgaaa tcatagacat accggcccct ggtcaacgtc ggacaggagg
     2881 tgggctccgc ggctatcacc cgtatctctt tcccgtggat cttgtcacgg atgaaaggga
     2941 aggctatgcc ggcgaagttg ctgccaccgc ccgcgcaccc gatgacgaca tcggggtatt
     3001 ctcccgcgag ctcgagctgc cgctgtgctt caaggccgat aacggactgg tggaggagga
     3061 cgtggttgag gacgctgccc agggagtact tcgtgttctt cgaggtcacc gcgtcctcga
     3121 tggcctcact gatcgccatt ccgaggcttc cagggtggtc cgggttccct tcgaggaacg
     3181 cgcgcccggc tttcgtgtcg ggactcgggc tgggcacgca cttccctccc caggtctcca
     3241 tcatgacgcg ccggtacggc ttctggttga agctcacacg gaccatgtag atcttcagct
     3301 cgatgccgaa ctggttgcag gcgaaggcaa gggcgctccc ccactgtccg gcccccgtct
     3361 ccgtcgtgag cctcttgatg ccggcctcct tgttatagta ggcctgcgcg atggccgtgt
     3421 tcggcttgtg gcttcccggg gggctgaccc cttcgttctt ataatagatg tgggcgggcg
     3481 tcccgaggaa cttttcaagc cccgtggccc ggcacaatgg cgtcggcctc cacatgagga
     3541 gtctttccat gacctcaccg ggaatgtcta tgaacctctc ggtgctcacc tcctgctcga
     3601 tgaggttcat cgggaagatg gcctcgagca tgtccggcgt cacgggctcc cccgtttccg
     3661 ggttgagcgg cgggggcagg ggcgtctcaa gatcggcctg aatattgtac caggcccgcg
     3721 gcatgtcctt ttcattgagg aatattttct tatccatgat accctccttt ctttcggtaa
     3781 cagaaagggc catgagcttg tctgcccatg gcccttttca aataaaacgg ccatggacgg
     3841 tgtgaaaccg tccatggccg gaaaattacg gtctcacacc tacaatctcc gccaccacca
     3901 ccagttgttt gtcgctgcgt tcgtcataat gaggctttat aacatgggaa atggaggatg
     3961 tcaaaataaa agataatgac caatgaccaa attccaatga ccagataaac agacaataac
     4021 caaaaagaga acgatacagg cagtccattc tgtccttctc tttgttcaat tggttattgg
     4081 tacattggtc attatcctct tcattggtca ttggaatttg gtcaattggt cattctcttc
     4141 ttattggtca ttctcttcat aatcgtgtat actcttacag tttttatatc caccatcatg
     4201 ttcggaggtc aggaaatgaa attctttata gatacggcaa atataaacga gatcagaaag
     4261 ggaatggaga tggggctcgt cgatggcgtg accaccaatc cgtcacttct cgcaaaagag
     4321 aagaaggacc ccgacgccgt gctccgcgag atacttcaga ccgttcccgg gcccgtcagc
     4381 ctggaagtca tatcgaccac ggcggaaggc atgtgcgagg aggctcggaa actcgccgcg
     4441 ctgggcgaga acgccgtcgt caagataccc atgacgggtg agggtatccg ggccgtcagg
     4501 acgttgaccg acgagggcat caagacgaat gtgaccctca tctttcagcc cgttcaggcc
     4561 ctcattgccg cgaaggcggg cgccacgtac gtgagcccct ttatcggcag gctcgatgat
     4621 atatctcaga ggggcatgga tatcatcgag aacatcgtta ccatattcta caactacgcc
     4681 tttgagacgg aggtcatcgt cgcaagcatc cgtaacccca cgcacgtcct cgacgcggcc
     4741 cttgtcggcg ccgatattgc caccatcccc ttcaacgtcc tctcgcagct catgaatcat
     4801 cccctgacgg acatcggcct gcagaaattc ctcaaggact gggagggcat caagaaatag
     4861 gggccttctt cagggtctct tcatccagga gttccctcac cttcttcagg aggtcccggg
     4921 gcaggatggg cttggctata tactctatcg tcgcgtcaag gatgcccttt tggtggatga
     4981 tgtcgtcagt gtagccgctc atgaaaagaa ccctgatatc ggggtccatg gacttcagtc
     5041 tatcgtaggc ctctttgccg ttcatcttcg gcatgacgac atcgaggatg atgaggtccg
     5101 cccggcaccg gtgttgtgct tcgatggcgt cgagtccatc caccgcctcg aagaccctgt
     5161 agccctgctc tgaaagaacg gtgcgcagga ggttcctcag ttcctcgttg tcctccacga
     5221 caagaatttt ttcgctgccc ctcgggtcgt cggcgacggt ctgtgcctct ttatcaggaa
     5281 cggcctcgac tatgggaagg aacacttcga aggtcgtccc gtgacccaac tcgctggcaa
     5341 cgctgatgga accattgtgc tgttggatta tcccgtagac tatcgcaaga cccagccccg
     5401 ttcctttccc cgattccttt gtcgtgaaga agggctcgaa gaccttctcc ttcgtcctgg
     5461 cgtccatgcc gacacccgtg tcggaaacgc tgatgcaggc gaaccggttt cccctctctc
     5521 cgtcagtccc gttcaccctt ttcgtgctga tcgtgaggat gccacctgtg ggcatggcgt
     5581 cccgggcatt ggccacgagg ttcatgagga cctggtcgat ctggacgagg tcggccatca
     5641 cgataaggaa acccggacac agctccgtcc tgaggacgat gtcctcggtg atgagccgcg
     5701 aaagcagctt cttagtcccc gtgatcgctc cattgacatc catcggcttc ggatcgatga
     5761 tctgcttcct gctgaaggca aggagactct gcgtgaggat ggccgccttc ccggatgacg
     5821 cgacgatctg gtcgacgtac ttgcgaacgg gatcgtcggg cgggagcttg atctggacga
     5881 ggttcgaata gccgatgatg gcgctgagga tgttattgaa gtcatgcgct atcccgcccg
     5941 caagctggcc tatgacctcc atcttctgcg
//
LOCUS       DAOQTR010000055         5952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14733_length_5952_cov_3.793454, whole genome shotgun sequence.
ACCESSION   DAOQTR010000055 DAOQTR010000000
VERSION     DAOQTR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14733_length_5952_cov_3.793454"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1305
                     /locus_tag="PKN85_02235"
     CDS             49..1305
                     /locus_tag="PKN85_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT43248.1"
                     /translation="MKGPAAILLAGGVGSRLNILVRHRAKPAVPFGGIYRIIDFTLSN
                     IGNSEMTSVAVLTQYKPLSLMDHIGDGSSWDLSGRSRSVKIFPPKTGEEDWDWYKGTA
                     DAVRQNLSFVVSAESDEIMILSGDHVYHMDYRRMVSFHREKKARVTIAAIPVPWEETY
                     QFGTAVTDDSNRIVSWEEKSPRARSNLASMGIYVFDRKYLIDLLEESKEDDFGHHIIP
                     RALEEKVVFAYPFDGYWRDVGTVQAYWDANMDLLDPSSGIDPGSWRTMTNISAREPAF
                     DRPPVLVSSGARVVRSILSPGCTVEGHVENCVLSPGVAVERGAFVRDSIIMHDCRIGE
                     GARVERCIFDKEVVVGAHTTVGIGDPKTVNRTHPEHLYSGLTIIGKQSIIPGDITIGT
                     NCIINPMLGSRSFTGEVGSGTTITGA"
     gene            1321..2583
                     /locus_tag="PKN85_02240"
     CDS             1321..2583
                     /locus_tag="PKN85_02240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT43249.1"
                     /translation="MADTAIKREDIQARVTRIGKADILVGIPSYNNARSIGHVVRAVQ
                     AGLAKYFPDRKTVLVNSDGGSTDGTMDVVHDTTIDDFRSILLSHRVESFSKMTIPYHG
                     IPGKGSAFRTIFEIAQALDVKACAVVDSDLRSITPEWIELLVKPVMEDGYDYVSPLYH
                     RHKYDGTITNSIVYPLTRALYGRNVRQPIGGDFGFSGKLAGFYLSKDVWETDVARYGI
                     DIWMTTTAIANDFRVCQAFLGAKIHDAKDPGADLSAMLYQVVGATFGLMEEYPRTWKS
                     VTRTQPVPVFGFTYEVGLEPVSVNIQRMIDKFTLGARELVPVWENVLSEETLGFLKEL
                     ADAPQDNFHMPDAVWVDIIYSFALAAHRNLLNKEHLLKSLTPLYIGRTASFVIEAWES
                     SGPEVEEKIENLCGVYEKLKPSFSDRWK"
     gene            2602..3309
                     /locus_tag="PKN85_02245"
     CDS             2602..3309
                     /locus_tag="PKN85_02245"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43250.1"
                     /translation="MNFLQRVIIEPFESSYEKFLTFMPNFLTSLALLIVGIVLAAVLR
                     MVFLRIFRAINLDKAAQRLGLFDMLLKGGIRDSLSAFIARIIGWLVIFTFAVVAVQTL
                     KIPTVEHLLERFFLYLPNVFTAAVILLFGYLFSNFFGRAALIASVNAGIQRAGLVGRL
                     VKITVFILSATMAMEQLGIGRDTVLIAFAIIFGGIVLALAIAFGFGGRDIARTYLQRT
                     VGKEKENEENRDDINPL"
     gene            3446..4480
                     /locus_tag="PKN85_02250"
     CDS             3446..4480
                     /locus_tag="PKN85_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43251.1"
                     /translation="MIEFRMVESESTFLDPRKGFEPTTVRFQVRVDPLTGRTCHFSHF
                     GAVKAQRLDLAGYHDPAVKGFCPFCAEHRERTTPRFTPDVVPEGRLENGEALLIPNLF
                     PYDIHSGVVVMTDDHVVGLKDFTRKRLDDAFSLGIDFLRRIADLDGSLPYHLMTWNYM
                     PPSGGGLVHPHQQYFATACPGNQFMDELSASRRFAERHRQNYWYALARQEATGNERYI
                     ARIGSGHWIASFVSLGLLGEIMCIFPDVFSLQDLTDDDIDDLVSGLERVFRYFEEAGV
                     FSFNASLCLGPRDQEHFSCYFRIVPRTFLNTRDFAPDLNFFQAILSEPICVVLPEDLC
                     REVKPYFNQG"
     gene            4735..5307
                     /locus_tag="PKN85_02255"
     CDS             4735..5307
                     /locus_tag="PKN85_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT43252.1"
                     /translation="MDIIELVTKNRSHRRFQESEPITMEQLEGLVELARLSPSAANLQ
                     PLKFVLSNDPARNASIFSCLAWEDYIRNSRGPAEGRRPSGYIVVLGDTTITKDFSCNH
                     GIAAQSILLGATEMGLGGYMFAGVDKARLATLLRIPREYEILLVIALGVPRERVVVTE
                     ARDGNIAYWRDINKVHHVPKRPLAEIILDL"
     gene            5386..>5952
                     /locus_tag="PKN85_02260"
     CDS             5386..>5952
                     /locus_tag="PKN85_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT43253.1"
                     /translation="MLELVTKSRSYRRFHEDRTISLQQLKGLVELARLSPSARNLQPL
                     KFILSHDSRKNAAIFSCLAWAAYLKDWPGPEEGERPSAYIIILGDGSLTKDFGCDHGI
                     AAQSILLGATETGLGGCILGNIQRDRLKALLKIPDRYEILLVVALGAPKEEVIIDEVK
                     EGDIKYWRDADKVHHVPKRSRGELILRPP"
BASE COUNT         1278 a         1781 c         1667 g         1226 t
ORIGIN      
        1 ggccgacgcg ttctcgaaga aggagtatct cagcggggag gtggtccaat gaaaggtccg
       61 gccgcgatcc ttctggcagg cggagtggga agcaggctca acatcctcgt ccggcacagg
      121 gcgaaacctg ccgtcccttt cggtggcata tacaggataa tcgacttcac cctcagcaac
      181 atcggcaatt cggaaatgac cagtgtggcc gttctgacgc agtacaagcc tctttccctc
      241 atggaccaca taggcgacgg ctcctcctgg gacctgtcgg gccgctcgcg cagcgtcaag
      301 atcttccccc ccaagacggg agaggaggac tgggattggt acaaaggcac agccgacgcc
      361 gtgcgccaga acctttcctt cgtcgtctcg gccgaaagcg atgagatcat gatcctgtcg
      421 ggcgaccacg tctatcacat ggactaccgc cggatggtct cctttcaccg ggagaagaaa
      481 gcccgcgtca ccatcgccgc cattcccgtg ccctgggagg aaacatacca gttcgggact
      541 gccgtcacag acgacagcaa caggatagta agctgggagg aaaaatcgcc aagggcaagg
      601 tccaacctcg cctcgatggg gatatatgtc ttcgacagga aatatctgat cgatctcctc
      661 gaggaatcca aggaagatga tttcgggcac cacatcatcc cccgggccct tgaggaaaag
      721 gtcgtcttcg cttatccttt cgacggatac tggcgcgacg tgggcaccgt tcaggcgtac
      781 tgggacgcga atatggacct tcttgaccca tcctcgggga tagacccggg atcctggagg
      841 acaatgacaa acataagcgc ccgggaaccg gccttcgaca ggccacccgt cctggtatct
      901 tccggcgccc gggtggtgcg ttccatcctg tcgcccggct gcaccgtcga ggggcatgtg
      961 gagaactgcg tcctgtcgcc cggagtcgcg gtcgaacgcg gcgccttcgt tcgcgactcc
     1021 atcataatgc atgattgccg cataggtgaa ggggcacgcg tcgaacgctg cattttcgac
     1081 aaggaggtgg tcgtgggtgc gcataccacg gtcggtatcg gggacccgaa gacggtcaac
     1141 cggacccacc cggaacatct ctactcgggc ctcaccatca tcggaaaaca gtcgatcatc
     1201 cccggagaca tcaccatagg cacaaactgc atcatcaacc ccatgctggg ctccagatcc
     1261 ttcacgggag aggtcgggag cggaacgacc ataactggag cgtgaaagcg aaaggaacat
     1321 atggctgaca ccgcgatcaa gagagaggac atccaggcgc gggtgacccg gatcggcaag
     1381 gccgacatac ttgtgggcat ccccagctac aacaacgccc gatccatcgg ccacgtggta
     1441 cgggccgtgc aggcaggtct cgccaagtat ttccccgacc gcaagacagt cctcgtgaat
     1501 tccgacggag gctcgacgga cgggacgatg gatgttgtcc atgacacgac gatcgatgat
     1561 ttccggtcga tcctcctttc ccaccgtgtg gaatcctttt ccaagatgac catcccctac
     1621 cacgggatcc ccggcaaggg aagcgctttc aggaccatct tcgagatcgc gcaggccctc
     1681 gacgtgaaag cctgtgccgt ggtggactcc gacctgcgca gcatcacccc cgagtggata
     1741 gaacttctcg ttaagcccgt catggaggac ggctatgatt acgtctctcc cctttaccat
     1801 cgccacaagt acgacgggac gatcacgaac agcatcgtct atcccctgac acgggccctc
     1861 tacggcagga acgtgcgcca acccataggc ggtgacttcg ggttctcggg gaaactcgcg
     1921 ggattctatc tgtccaagga cgtctgggaa accgacgttg cccgctacgg catagacatc
     1981 tggatgacga caacggccat agccaatgac ttccgggtct gccaggcctt tctcggggcc
     2041 aagatccacg atgccaagga cccgggggcg gacctttcgg ccatgctcta ccaggtcgtg
     2101 ggggcaacct tcggcctcat ggaggaatac ccgagaacat ggaagagcgt gacacggaca
     2161 cagccggtgc ccgtattcgg gttcacctac gaggtcggcc tcgaaccggt gagcgtcaac
     2221 atccagagaa tgatcgacaa gttcacactg ggcgccagag aacttgtccc cgtgtgggag
     2281 aacgtcctgt ccgaagagac cctcggcttc ctcaaggaac tggccgacgc gccgcaggac
     2341 aatttccaca tgcctgacgc ggtgtgggtg gacatcatat acagcttcgc cctggccgcg
     2401 catcgcaacc tgctcaacaa ggaacacctt ctcaagtcgc tgaccccgct gtacatagga
     2461 cgcacggcgt cattcgtgat cgaggcctgg gaaagcagcg gacccgaggt ggaagagaag
     2521 atagagaacc tctgcggggt atacgagaag ctcaagccgt ccttctcgga ccgctggaag
     2581 taaaacgaaa gggggcttta catgaatttt cttcagaggg tcattataga gcctttcgag
     2641 agctcttacg agaagttcct cacattcatg cccaatttcc tcacctccct ggcgcttctc
     2701 attgtgggca tcgtccttgc tgcggtcctc aggatggtct ttctaaggat cttccgggcc
     2761 ataaacctcg acaaggcggc ccaacgcctc gggcttttcg acatgctcct caaaggcggg
     2821 atcagggact ccctttcggc cttcatcgca aggataatcg gatggctcgt catcttcacc
     2881 ttcgccgtcg tggcggtgca gacgctcaag atacccaccg tcgagcacct cctggagcgt
     2941 ttcttcctct atctccccaa tgtcttcacg gcggcggtga tccttctctt cggctatctt
     3001 ttcagcaact ttttcggacg cgccgcgctc atagcgtcgg tcaacgcggg aatacaaagg
     3061 gccggcctcg tcggcaggct ggtcaagatc accgtcttca tcctgtccgc cacaatggcg
     3121 atggaacagc tcgggatcgg ccgtgatacc gtgctcatag cctttgccat tatcttcgga
     3181 ggcattgtcc tggccctcgc catcgccttc ggcttcggcg gaagggacat cgccaggacc
     3241 tatctgcaaa ggaccgtggg gaaagagaag gagaatgaag agaaccggga cgacatcaat
     3301 ccgctgtaga gttttaagtt gtcaggtttt aggttttaag ttgaagaccg tcgttgtcct
     3361 gacccttatc cgttctttcc attgacaggc ggttggggat tctgctctta ttttgagaaa
     3421 tacaacgaca gggagatgac ggcgcatgat cgagttcagg atggtggaat cggaaagtac
     3481 tttccttgac ccgcgaaagg gcttcgagcc cactaccgta cgtttccagg tgcgtgtcga
     3541 tccgttaacg ggcaggacct gtcatttttc tcatttcggc gccgtcaaag cccagagact
     3601 cgatctggcc gggtatcacg atcccgccgt aaagggtttc tgtcccttct gcgccgaaca
     3661 tcgcgaaagg acaacgccga gattcacgcc cgatgtcgtt cccgagggga ggctggagaa
     3721 cggggaggcg cttcttatac ccaatctatt cccctatgac atccacagcg gtgtcgtcgt
     3781 catgaccgac gatcacgtcg tcggcctgaa ggacttcacc cgaaagaggc tcgatgatgc
     3841 cttttccctg ggcatcgact tcctgcgccg catagcggat ctcgacggtt cgttgcccta
     3901 tcacctcatg acatggaact acatgccccc ttcgggggga ggcctcgttc atccccatca
     3961 gcaatacttc gccaccgcat gccccggcaa ccagttcatg gatgagctgt ccgcatcacg
     4021 acgcttcgcg gaacgccaca ggcagaatta ctggtatgcc ctcgcgagac aggaggcgac
     4081 aggcaacgag cggtatatcg cacggatcgg ttcgggacac tggatcgctt ctttcgtctc
     4141 tctcgggctg ctcggagaga tcatgtgcat ctttcccgac gtcttttccc tccaggacct
     4201 caccgacgac gatatcgacg acctcgtttc cggactcgag cgcgtcttcc ggtatttcga
     4261 ggaagcgggg gtcttcagct tcaacgcgtc gctctgtctc ggcccccgcg accaggagca
     4321 cttcagctgc tacttccgca tcgtcccccg gacgttcctc aacacgcgtg actttgcccc
     4381 ggacctcaat ttcttccagg ccatcctctc cgaacccatc tgcgtggtcc tcccggaaga
     4441 cctctgcagg gaggtaaagc cctacttcaa ccagggatag ttgcaggttc aagggttcaa
     4501 agacatttca agagttcaag ggctcaagag taaaagaaga aaacaacgga cgcttttggc
     4561 gtgtaagcac gattcgaggg aaggaccgcg aagggggatc ggttgtcaat actattcgcg
     4621 taccggccaa aggcatccgg cgccttttgg tcttctactc ttgaactctt gaacttttga
     4681 acccttgaac tttcttttgc ttgcatgccc ctccttctat cctatagttc ctgtatggat
     4741 atcatcgaac tggtcacgaa gaacaggagc caccggcggt ttcaggagag cgaacccata
     4801 accatggaac agctggaggg cctcgtggag cttgcccgtc tttcgccgtc ggccgcgaat
     4861 ctccagcctc tcaagttcgt tctctccaac gaccccgccc gcaacgcgtc cattttctcc
     4921 tgtcttgcgt gggaggatta tatcaggaac tcccgcgggc ctgccgaagg ccgtcggccg
     4981 tcgggataca tcgtcgtcct cggtgatacc accatcacga aggacttctc gtgcaaccac
     5041 gggatcgccg cgcagagcat actcctcggc gccacggaga tgggtctggg cggatacatg
     5101 ttcgccgggg tggataaggc caggcttgcg actcttctcc gtataccccg ggaatacgag
     5161 atactgctcg tcatcgccct gggcgttccc agggaacgtg tcgtcgtcac ggaggccagg
     5221 gacggcaaca tcgcctattg gcgcgacatc aacaaggtcc accacgttcc taaacgcccc
     5281 ctggcggaga tcatactgga tctttgaaaa aatgttccag gttcaagggt tccagagttt
     5341 caggtttttg aatcttacgt cacagcgatg ggagatggca tgactgtgct ggagcttgtt
     5401 acgaagagca ggagttaccg gaggtttcac gaggacagga cgatcagttt gcagcagctg
     5461 aaggggcttg tcgagcttgc ccgcctttcg ccctcggcga ggaacctgca gcccttgaag
     5521 ttcatcctgt cccatgactc ccggaagaac gccgccatat tctcgtgcct tgcatgggca
     5581 gcttatctca aggactggcc gggccccgag gaaggcgaaa ggccttccgc ctatatcatt
     5641 atcctcgggg acgggtccct cacaaaggac ttcggctgcg accacgggat cgccgcccag
     5701 agcattttgc tgggagcgac ggagacggga ctcggcggat gtatactcgg caatatccag
     5761 agggaccgcc tgaaagccct tctgaagata ccagatcggt acgagatcct ccttgtcgtt
     5821 gccctcggag cacccaaaga agaggtcatc atcgacgagg tgaaggaggg ggacatcaag
     5881 tactggcgcg acgccgacaa agtccaccac gtgccgaaac gctcccgggg ggaactgata
     5941 ctgcgaccac cg
//
LOCUS       DAOQTR010000056         5909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14878_length_5909_cov_3.014008, whole genome shotgun sequence.
ACCESSION   DAOQTR010000056 DAOQTR010000000
VERSION     DAOQTR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14878_length_5909_cov_3.014008"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /gene="lon"
                     /locus_tag="PKN85_02265"
     CDS             <1..1784
                     /gene="lon"
                     /locus_tag="PKN85_02265"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNT43254.1"
                     /translation="VLEIADPVERLRKLSEILGKEIELLNMQAKILSQAKEEMSKSQR
                     EYFLREQMRAIRTELGEGDERTEEVEELRKRIKKAKMPKEVEKEAKKQLERLDMMHPD
                     AVESTMVRTYLEWLVELPWSKSTKDNIDIKKARAILDKDHYDLDKVKDRILEFLSVMK
                     LKADMKGPILCFVGPPGVGKTSLGKSIARAIGRKFSRISLGGMKDEAEIRGHRRTYVG
                     SMPGRIIQYIKIAGTNNPVFMMDEIDKIGNDFRGDPASALLEVLDPEQNNAFSDHYLN
                     VPFDLSKVMFITTANQMDTIPSALRDRMEVIDIPGYTEKEKLEIARRYLVPKQLSENG
                     LTGARLSITDKAMEKIINEYTREAGLRNLEREIAMVARKVARRVAEGDKKKVTLTPKN
                     VHDFLGPVKYLPDSQMEQDYVGVASGLAWTEFGGDVLYVEASCRKGKKELTLTGNMGE
                     VMKESVQAALTYIKSKAEALGIDKAIFDDLEMHVHVPQGAIPKDGPSAGITMAVAMIS
                     AITGRPINRKIAMTGEITLTGRVLPIGGLKEKTLAALRNRMKRVLIPHENDHELVEIP
                     QYVKKKITFLSVKTMDDVVAAVFGKKV"
     gene            1788..2801
                     /gene="amrS"
                     /locus_tag="PKN85_02270"
     CDS             1788..2801
                     /gene="amrS"
                     /locus_tag="PKN85_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNT43255.1"
                     /translation="MKEASFYEKRDGKTIHCFLCRHNCVIADGKRGLCGVRENRDGTL
                     YSLVWGLPCAYHVDPIEKKPLFHFYPGSKAFSVATVGCNFRCLHCQNHDISQGPRENG
                     RVFGETVSPGEIVEQALAARCASISYTYTEPTIFFEYAFDIAREAREKGMANNFVTNG
                     YIEEAPLRAIAPYLDAANIDLKGFSEVFYRDVCGARLSGVLDSIRLYRELGVWIELTT
                     LIIPGHNDSDEELRSIARFIAKDLGVEVPWHVSAFYPTYKLLDVEPTSPQSLARAREI
                     GRDEGLRYVYEGNIPGSGGENTSCHNCGKTVIGRYGYSITEYNLKGGACKFCGTVLDG
                     RGF"
     gene            2910..4538
                     /locus_tag="PKN85_02275"
     CDS             2910..4538
                     /locus_tag="PKN85_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT43256.1"
                     /translation="MRFFENIRIGKRLGIGFAIFIVIIALLALLGFKIIDDVDRKAGH
                     IVEAALEKTILANTVMGAIYNAYNSMGVIAFATDAAVIEAEKENLGGMRQTYGEALAK
                     LEKLEQDPKGKDLIARFKQTTAKAKDSNNRIIEYAGGKEQARAIETYINENKPATKEI
                     ITTLQSIIRYQEDNVRGQFASIREANRKYTLILLVFGIAAVVLGIILSVTITRSIVVP
                     IRGNTRAVKAMAKGNLAFEVPTDRKDEFGDEMKAAKEMVEKWREIVGSLKSAAASISS
                     AGVQLSASAEQMSKGSGEQANRSSQVAAASVEMSQTVVDIARNTNNIANSAANTVKIA
                     KEGEEIVNQATKEVEEIARTGGDSAESIRTLGERSNQIGEIVNVIDDIADQTNLLALN
                     AAIEAARAGEQGRGFAVVADEVRKLAERTANSTSEIGGMINAIQGDVERAVMTMNTVA
                     KKVETGVDLSGKAGQSLRVIVTSVDDLLGMVQQIASATDEMSATSEEINRDIEQIAAV
                     SRETSASSEQTAQASGELAKLSNDLEDIVSRFRV"
     gene            4735..>5909
                     /locus_tag="PKN85_02280"
     CDS             4735..>5909
                     /locus_tag="PKN85_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNT43257.1"
                     /translation="MSVKKGLSTALLLLMFTLVALPAFAGEADIILPDLSTVTFTIFG
                     SVVSGVSIMYFGIVICFIGLIFAIMQASQTKKMPAHKAMLEVSAVIWETCKSYLAQQG
                     KFLFILWLLIAACMVYYFMGLSHAPFGNMVIILICSILGILGSYGVAWFGMRINTWAN
                     SRTAFASLRGKPVEVVNIPLTSGMSVGLLLVSVELFFMICILVFLPKELVGPSFIGFA
                     IGESLGASALRIAGGIFTKIADIGSDLMKIVFKLPEDDPKNPGVIADCTGDNAGDSVG
                     PTADGFETYGVTGVALVAFLALALAANPMMSGTLIIWIFAMRILMILTSLVSYFINRG
                     ITSSVYGSKTKFDFEQPLTNLVWITSAVAIIVTFWASYMLLGDLNAQYPGLWWALAI"
BASE COUNT         1365 a         1694 c         1752 g         1098 t
ORIGIN      
        1 aggtcctgga gatcgccgat cccgtcgagc gcctgaggaa gctctcagag atcctgggca
       61 aggagatcga actcctaaat atgcaggcca agatactctc ccaggccaag gaggagatgt
      121 ccaagtccca gagggagtat ttcctcaggg aacagatgcg cgccatccgc accgagctcg
      181 gcgagggcga cgagcgtacg gaggaggtcg aggagctccg caagcgcatc aagaaggcga
      241 agatgcccaa agaggtggag aaggaggcga aaaagcagct cgaacgcctc gacatgatgc
      301 accccgacgc cgtcgaatcc acgatggtgc ggacatacct cgaatggctt gtcgagctcc
      361 cctggagcaa atccacgaag gacaacatcg acataaagaa ggcgcgcgcg atcctcgata
      421 aggaccacta cgacctcgac aaggtgaagg accgcatcct ggagttcctg agcgtcatga
      481 agctcaaggc cgacatgaag ggccccatcc tgtgctttgt gggaccgccg ggtgtgggca
      541 agacgtcgct cggcaagtcc atcgcccggg ccatcggccg caagttttcc cgcatatcgc
      601 tgggcggcat gaaggacgag gcggagatcc gcggccacag gcgcacgtac gttggctcca
      661 tgccgggcag gatcatacag tacatcaaga tagccgggac gaacaaccct gtcttcatga
      721 tggacgagat agacaagatc gggaacgatt tccggggaga ccccgcgagc gcgctcctcg
      781 aggtgctcga tcccgagcag aacaacgcct tcagcgatca ctacctgaac gttcctttcg
      841 acctgtcgaa ggtcatgttc atcaccaccg cgaaccagat ggacaccatt ccctcggcct
      901 tgcgcgacag gatggaggtc atcgatatcc ccggatacac cgaaaaggag aagctcgaaa
      961 tagccaggcg ttacctcgtc cccaagcagc tctccgagaa cggcctcacg ggcgcgcggc
     1021 tcagcataac cgacaaggcc atggagaaga tcattaacga atacacccgc gaggcgggtc
     1081 tgaggaacct cgaaagggag atcgccatgg tcgcccggaa ggtggcaaga agggtcgccg
     1141 agggggacaa gaagaaggtg accctcaccc cgaagaacgt ccacgacttc ctggggcccg
     1201 tcaagtacct gcccgacagc cagatggagc aggattatgt gggcgtggcg agcgggcttg
     1261 cctggacgga gttcggcggc gacgtgctct acgtcgaggc ctcctgccgc aagggcaaga
     1321 aggaactgac cctcacgggc aacatgggcg aggtgatgaa ggaatcggtg caggcggcgc
     1381 tcacctatat caaatcgaag gcggaggctc tcggcatcga taaggccatc ttcgacgatc
     1441 tggagatgca cgtccacgtg ccccagggcg ccatccccaa agacggtccc tcggcgggca
     1501 tcaccatggc cgttgccatg ataagcgcca tcacgggacg tcccatcaac aggaagatag
     1561 cgatgacggg ggagatcacc ctgacgggca gggtccttcc cataggtggt ctcaaggaaa
     1621 agaccctcgc cgccctgcgc aaccggatga aaagggtcct catcccccac gagaacgacc
     1681 acgagcttgt cgagataccc cagtacgtga agaagaagat caccttcctt tccgtgaaga
     1741 ccatggacga cgtggtggcg gcagtttttg gaaaaaaggt gtgaaacatg aaagaagcat
     1801 ccttttatga gaagagggac ggtaagacca tccattgttt tctgtgccgc cacaactgcg
     1861 tcattgccga cggcaaaagg ggcctgtgcg gtgtccgcga gaacagggat ggcaccctct
     1921 acagccttgt ctggggcctt ccctgtgcct accacgtgga tcccatagaa aagaagcccc
     1981 tctttcactt ctatcccggc tcgaaggcct tctcagtggc cacggtggga tgcaacttcc
     2041 gctgcctcca ctgccagaac catgatatct cccagggtcc ccgcgagaac gggagggtct
     2101 tcggagagac ggtgagcccc ggggagatcg tggagcaggc gctcgcggca cggtgcgcga
     2161 gcatctccta cacctacacc gaaccgacca tcttcttcga atacgccttc gacatagcac
     2221 gcgaagcccg ggaaaagggc atggcgaaca acttcgtgac caacgggtac atcgaggagg
     2281 cgccgcttcg ggccatcgcc ccctatctcg acgcggcgaa catcgacttg aaaggcttca
     2341 gcgaggtctt ttacagggac gtatgcgggg ctcggctttc cggtgtcctc gattcgatac
     2401 gcctttacag ggaactgggg gtctggatag agctgacgac gctcatcata ccggggcaca
     2461 atgacagcga tgaggaactg aggtccatcg cgcggttcat agcgaaagac ctcggcgtgg
     2521 aggtgccctg gcacgtgagc gccttctatc ccacctacaa gctcctcgac gtggagccga
     2581 cgagcccgca gagcctcgcc cgggcccggg agataggccg cgatgagggg ctccgttatg
     2641 tctacgaagg caacatcccc ggctccgggg gagagaacac ctcctgccac aactgcggca
     2701 agacggtcat agggcgttac gggtactcga tcacggagta caacctgaag ggcggggcct
     2761 gtaaattctg cggcaccgtt ctcgacggca gggggttctg acccgccggg caaggccccc
     2821 ggcgaagaaa tgtcttgaaa taaagtcact cccttaatta aatatagaat acctaatcct
     2881 taaacaaatg aagcaacgaa gggggagaca tgcgtttctt tgagaatatc aggataggca
     2941 aacgtctcgg catcggtttc gccatcttca tcgtcatcat agcgctcttg gcccttctgg
     3001 gtttcaagat catcgatgac gtggacagga aggccggcca tatcgtcgag gcggccttgg
     3061 agaagaccat cctcgccaac accgtaatgg gggcgatcta caacgcctac aattccatgg
     3121 gtgtcatcgc ttttgccacg gacgccgccg tcatcgaggc ggaaaaggag aacctgggcg
     3181 ggatgcggca gacctatggc gaggccctgg cgaagctcga aaaactcgag caggacccca
     3241 agggaaagga cctcatcgcc cgcttcaaac agacgacagc gaaggcgaag gactccaaca
     3301 accggatcat cgagtacgcg gggggcaagg aacaggcaag ggcgatagag acatatatca
     3361 acgagaacaa acctgccaca aaggagatca tcacgaccct gcagtccatc atccggtacc
     3421 aggaggacaa tgtccgcggc cagttcgcgt ccatccgcga ggccaacagg aagtacaccc
     3481 tgatcctcct cgtcttcggc atagccgccg tggtgctcgg catcatcctt tccgtcacga
     3541 tcacccggag catcgtcgtg cccatccgcg ggaacacccg ggcggtgaag gccatggcga
     3601 aagggaacct cgcctttgag gtccccacgg acaggaagga cgagttcggc gacgagatga
     3661 aggcggcgaa ggaaatggtg gagaagtggc gcgagatcgt cgggagcctc aaatcggccg
     3721 cggcgagcat atcctccgcc ggtgtccagc tcagcgccag cgcggagcag atgtcgaagg
     3781 gctccgggga gcaggccaac cggtcgtccc aggtggccgc ggcctccgtg gagatgtcac
     3841 agacggtagt cgacatagcg cgcaacacga acaacatagc gaactcggcg gcgaatacgg
     3901 tgaagatagc gaaggaaggc gaagagatag tcaaccaggc cacgaaggaa gtggaggaga
     3961 tagcccggac cggcggcgat tccgccgaat cgatacggac cctcggggag cgttcgaacc
     4021 agatcggaga gatcgtcaac gtcatcgatg acattgccga ccagacgaac ctcctggcgc
     4081 tcaacgccgc gatcgaggcc gcccgggccg gcgagcaggg caggggtttc gcggtcgtgg
     4141 ccgacgaggt gcgcaagctg gcggagcgca ccgcgaactc cacctcggag atcggcggca
     4201 tgatcaacgc gatccagggc gatgtggaac gtgccgtcat gaccatgaac accgtggcga
     4261 agaaggtgga aacgggcgtc gacctctccg ggaaggcggg ccagtcgctc cgggtcatcg
     4321 tgacgagcgt cgacgacctc ctgggaatgg tccagcagat cgcttcggca acggatgaga
     4381 tgagcgccac atcggaggag atcaacaggg acatcgagca gatcgcggcc gtctcccggg
     4441 agacgtcggc aagctcggaa cagacagccc aggcatcggg agagctggcg aagctctcga
     4501 acgacctcga ggatatcgtc agccgcttcc gggtgtgaaa ggcgcctcgc cattacgcct
     4561 aacataatgt tttcatggaa gaacgaagct tgtcagcttt gtgaagggta ttgtgaattt
     4621 tttcctatgt ttggttgaca atcgaaaaag aaaatgttta atatagggag ccaatccata
     4681 ccgttccaca ggtaattatg gttattcatt attcatctca aaggaggtcc atagatgagt
     4741 gtcaaaaaag gtttaagcac tgcactattg ttgttgatgt tcactctcgt cgctttgccg
     4801 gcgttcgccg gtgaggcgga catcatcctg ccggatctgt cgacggtcac gttcactatc
     4861 ttcggcagcg tggtgagcgg tgtgagcatc atgtatttcg gtatcgttat ttgttttatc
     4921 ggtctgatct ttgccatcat gcaggcaagc cagacaaaga agatgcctgc ccacaaggca
     4981 atgcttgagg tttccgctgt tatctgggag acgtgtaaat cctatctcgc ccagcagggc
     5041 aagttcctct tcattctctg gctcctgata gccgcctgta tggtctatta cttcatgggc
     5101 ctgtcccatg ctccctttgg caacatggtc atcatcctca tctgctccat cctcggtatc
     5161 ctcggttcct acggtgtggc gtggttcggt atgaggataa acacatgggc aaactcccgg
     5221 acggcctttg cgtcgcttcg cggcaaaccg gttgaagtcg tcaacatccc cctgacctcg
     5281 ggaatgagcg tcgggctcct tcttgtgagc gtcgagcttt tcttcatgat atgcatcctt
     5341 gttttcctgc cgaaggaact cgtcggaccc agcttcatcg gtttcgcgat cggtgagtcc
     5401 ctcggtgcca gcgcactcag gatcgccggt ggtatcttca cgaagatcgc cgacatcggc
     5461 agtgacctca tgaagatcgt cttcaagctt cccgaagacg acccgaagaa ccccggtgtt
     5521 atcgccgact gtaccggtga caacgccggc gacagcgtcg gccccacggc ggacggtttc
     5581 gagacctacg gtgtcacggg tgttgccctt gtcgccttcc tcgcccttgc tcttgcggca
     5641 aacccgatga tgtcgggtac gttgatcatc tggatattcg caatgcgtat cctcatgatc
     5701 ctcacctccc ttgtttccta tttcatcaac aggggcatca cgtcatctgt ttacggttca
     5761 aagacaaagt tcgatttcga acagcctctt accaacctgg tatggatcac ctcggctgtt
     5821 gcgatcatcg tcaccttctg ggcgagctac atgctcctcg gtgaccttaa tgctcagtat
     5881 ccgggactct ggtgggcact ggccatcat
//
LOCUS       DAOQTR010000057         5900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14911_length_5900_cov_3.182720, whole genome shotgun sequence.
ACCESSION   DAOQTR010000057 DAOQTR010000000
VERSION     DAOQTR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14911_length_5900_cov_3.182720"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..631
                     /locus_tag="PKN85_02285"
                     /pseudo
     CDS             1..631
                     /locus_tag="PKN85_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
     gene            816..1346
                     /locus_tag="PKN85_02290"
     CDS             816..1346
                     /locus_tag="PKN85_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43258.1"
                     /translation="MADRLRTLRRLVPVALCLLSVLLLSSCGDLQAKGIWSAIAGTRQ
                     DDVWYIDQTLAYSTHDKVTTSRAVLKFVPGQGSVLNEEIRECLDLNGIVPKDLSYFVA
                     FVSVDCRRELLHFSDMAFFDSEDNVIWRQQYEETYHIVPTADTSAGVISGYLCLNRPG
                     FLDTLKKKKPFLYLFP"
     gene            complement(1281..1799)
                     /locus_tag="PKN85_02295"
     CDS             complement(1281..1799)
                     /locus_tag="PKN85_02295"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNT43259.1"
                     /translation="MGFIEFAIFDSWFGTISVVRRERRIVRLDLSENDIYQEHRAVLK
                     RFPDAVESEQSFKTVRTLLHRYLKGQPVDFRDVEIDISDLPEFTQTVLEELRKIPYGE
                     TRSYLDIARRAGHPLGGRAVGQAVKRNPIPIIVPCHRVIRHDGSLGGFGLGEKIKKRL
                     LFLEGVKKTWSV"
     gene            complement(1857..2324)
                     /locus_tag="PKN85_02300"
     CDS             complement(1857..2324)
                     /locus_tag="PKN85_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT43260.1"
                     /translation="MGTKDLSAIIELAISKEQEAYDFYTELLSMVDDRAARDALQIVA
                     GEEKKHKEFLVNYRDKGYGDSGLKMTTVVDYKIAEHLEAPEPRAGMESKDVFLIAAHR
                     EKGAHEFYLGLASIHPAGPTKDMLLQMAGEELKHKEKMEYYYANAAFPQTSGG"
     gene            complement(2480..5179)
                     /gene="fdhF"
                     /locus_tag="PKN85_02305"
     CDS             complement(2480..5179)
                     /gene="fdhF"
                     /locus_tag="PKN85_02305"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4130..4132),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNT43261.1"
                     /translation="MKITINGKVCEATEGQTVLEVARANGFYIPTLCYLSGLTPTGAC
                     RICVVEVEGMRNLVASCSAPATEGMVVRTDTERVLAARRFIVELLVSCHPLDCMTCEK
                     TGDCTLQNLAYELGVTGSAMVGEKYDYPVDYSNPFIVRDYNKCILCLKCVRVCNEIQG
                     IEAIKTIQRGFDTKVATAYDTPLQESNCVFCGQCVQACPVGALTERKAKGLGRAWEMR
                     KVRTTCPYCGVGCQLELHLKGEQIVKVTAVEDGAPNKGRLCVKGRFGYDFIYSEDRLT
                     TPLIRENGSFREASWDEALDLVADKFKRIIAESGPDAIAGVSCARSINEDSYQMQKLF
                     RAVIGTNNIDHCARTUHAPTVAGLAQSFGSGAMTNSFGEFADAKMFLCIGTNMTEAHP
                     VAATFLKNALRKGAKLVVVDPRKQPLAKLADIYCQIKVGSDIAFLNGLMNIIINEGLY
                     DKDFVENRCDNFEAFKAKIMEYPPERAAEISGVDVETIRKVAHMLSEIKPAMLCYTLG
                     ITEHTTGKDNVMSTANLQLLLGNMGKPSAGVNPLRGQNNVQGACDMGALPNVFPGYQK
                     VIDPAAREKFEKAWNVGKLPENNGMMMPQMMESLADKKIRAFYVFGENLASTEPDIRK
                     VEHELSSAEFLICQDIFPTETTRFAHVILPAASWCEDDGTFTNSERRVNRVRKVKNPP
                     GNAKPNWWIFKEIARRMGQDWTSNSGPEIWDNEVSALAPALGGIKYDRIENDGLQWAC
                     PNTDHPGTAFLHRDGVFTHGKGIFNPVDWKAPAEVADKEYPFVLSTGRRLYQYHTRTQ
                     TGRSGLNFLMPEETADISVVDAENLGIKSDELVKVRSRRGEVEVRARVTAEVPPGMVW
                     MSFHFREGSANWLTNAVYDPATLTAEYKACAVTVEKIQDTATA"
     gene            5472..>5900
                     /locus_tag="PKN85_02310"
     CDS             5472..>5900
                     /locus_tag="PKN85_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT43262.1"
                     /translation="MEGFIKFFGTGGARFVALKQLRATGGLWLNYRKTNLYIDPGPGA
                     IVRIRAAKEDYDPSRLDGIVITHRHMDHANDVNILIEAMADGGFRKRGTLFCPADALS
                     GDSVVFDFAGKYLENIILLREGGEYALGDITFRTPVRHRHP"
BASE COUNT         1172 a         1710 c         1696 g         1322 t
ORIGIN      
        1 ggagggaagg ttcagcttca acgtgaaggg gggcaggtgc gagacctgcg ccggagaggg
       61 cttcgtgaag atagagatgc agtttctccc cgatgtttac atagtctgcg aggcgtgcaa
      121 agggaagcgt tacaaccgcg acacgctgga gatcctctac cgcggcaaga gtatcgcaga
      181 tgtcctggaa atgacggtca cgcaggcaat ggagttcttc gacgcccatc cccacatcaa
      241 gagcaagctc aaggtcctct acgaggttgg tctggggtat atccgcctgg gtcaggcggc
      301 gactacccta tccggcggag aggcacagag gatcaaactc tcccgtgaac tctccaaacg
      361 ggacacgggt aagaccttct atattctcga cgaaccgacg acaggcctcc atttcgtcga
      421 catcgagaag ctccttcacg tgctcaacga actcgtcgag aggggcaaca ccgtcgtcgt
      481 catcgagcac aatctcgacg ttatcaagag tgccgattat gtgatcgacc tcggtcccga
      541 ggggggcggc cggggaggaa cgatcatcgc ccgtggcacc ccggaagacg tcatgtccgc
      601 agagaatagt tacaccgggc tgtttctgaa gcggtatctt gaggccgacc ggggatcggc
      661 ggccggggag agggtggggg cgaaaagcgc ttcaggatct cggagttcta gggttccagg
      721 gtaagtgcac ccgacgaaag agcacgaacc gccctgggaa gtcactttgt caacagtctg
      781 tttaggctcc acggcaaagg tgcgataaac gaaatatggc cgatcgactg cgaacactgc
      841 ggcggcttgt ccccgtcgcg ctctgccttt tgtcggtgct gctgttgtct tcatgcggag
      901 atctgcaggc gaaggggata tggtcggcca ttgccgggac caggcaggat gatgtctggt
      961 atatcgatca gaccctcgca tactcgaccc atgacaaagt gaccacttcg agggccgtgc
     1021 tgaaattcgt ccccggtcag ggaagcgtcc tcaatgagga gatcagggaa tgccttgacc
     1081 tcaacgggat cgtcccgaag gacctttcct atttcgtagc gtttgtttcc gtcgactgcc
     1141 ggagggaact gctccatttt tcagatatgg ccttcttcga ttccgaggac aatgtgatct
     1201 ggcggcagca gtacgaagag acgtatcata tcgtgcccac ggcggatacg tccgccggcg
     1261 tcatctccgg ctatctctgc ctaaaccgac caggttttct tgacaccctc aagaaaaaga
     1321 agccttttct ttatcttttc ccctagaccg aatcccccca gggacccgtc gtggcggatg
     1381 accctgtggc aggggacgat gatggggata gggttcctct tcaccgcctg accgacggcc
     1441 cgtcccccca aggggtgtcc ggcgcgtctc gcgatatcga gatagcttct cgtttcgccg
     1501 taggggatct ttcgcagctc ttccaggacc gtctgcgtga actccggcag gtccgatata
     1561 tcgatctcca catcacggaa atccaccggc tgtcctttca ggtaccggtg gagaagggtg
     1621 cgcactgtct tgaaggactg ctccgactcg acagcatcgg gaaaacgttt cagtacggcc
     1681 cggtgctcct ggtatatgtc gttctccgaa agatcgagac gaacgatccg gcgctctctc
     1741 cttacaacgg agatggttcc gaaccaggag tcgaatatgg caaattcaat gaaccccaaa
     1801 gacttcctcg cggtgtgcac gcggcgctct caggagagat gtatcccctc gtgctgtcac
     1861 ccgccggatg tctgcgggaa agcggcgttc gcgtaataat actccatttt ctccttgtgc
     1921 ttcaattctt ccccggccat ttgcaggagc atgtccttcg ttggccccgc agggtgaatg
     1981 gaggcaagtc ccaggtagaa ctcgtgggcg cccttttccc gatgggccgc tataaggaag
     2041 acatccttgc tttccatacc ggccctgggc tcgggggcct caaggtgttc ggctatcttg
     2101 taatcgacga cggttgtcat tttgaggccc gagtccccgt agcccttgtc gcggtaattc
     2161 acgaggaact ccttgtgttt cttttcctcg ccggcaacga tctgcagggc gtctctggcg
     2221 gccctgtcgt cgaccatgct cagaagttcc gtgtagaaat cgtaggcttc ctgctctttt
     2281 gatatcgcca actcgatgat ggccgagaga tcttttgttc ccataaaccc tccttttcgc
     2341 ttgcacttat atattggcac actccgcccg ggcgcgcaag gtcctcagaa acgaagcccg
     2401 ccgtcgaaca cgaaagaagg aagcccccga gggtggattt cgggggctgt gagagtcttc
     2461 attgctgtcg tggcatatgc tatgcggtcg ccgtatcctg tatcttctcc accgtcacgg
     2521 cgcaggcctt gtattccgcg gtgagggttg ccgggtcgta caccgcgttg gtgagccagt
     2581 tcgctgatcc ttcccggaaa tggaaggaca tccagaccat gccgggaggc acctccgcgg
     2641 tgaccctggc ccggacctcc acttctccgc gccgcgaacg gacctttacg agctcgtccg
     2701 atttgatgcc gaggttctcc gcatcgacaa cggatatgtc agccgtttcc tcgggcatga
     2761 gaaaattgag gcccgagcgg cccgtctggg tcctggtgtg gtattggtac agtctgcggc
     2821 cagtgctgag gacgaagggg tactccttgt cggcgacctc cgccggcgct ttccagtcaa
     2881 cgggattgaa tatccccttg ccatgggtga acacaccgtc gcgatggagg aaggccgttc
     2941 cggggtggtc cgtattggga caggcccact gcagcccgtc gttctcgatg cggtcgtact
     3001 tgatccctcc gagcgccggg gccagggcag atacctcgtt gtcccatatc tcgggtccac
     3061 tgttcgatgt ccagtcctgc cccattctcc tggcaatttc cttgaatatc caccagttag
     3121 gcttcgcgtt tccaggcggg ttcttgacct tgcggacgcg gttgaccctt ctttcgctgt
     3181 tcgtgaaggt cccatcgtcc tcgcaccacg atgcggcggg caggatgaca tgggcgaagc
     3241 gtgtcgtctc cgtgggaaag atgtcctgac agatcaggaa ttcggcggag gacagctcgt
     3301 gctcgacctt acggatatcg ggctccgtgc tcgcgagatt ctcgccgaag acatagaatg
     3361 cccggatctt cttatctgca agcgattcca tcatctgggg catcatcata ccattgtttt
     3421 cgggcagctt cccgacgttc caggccttct cgaacttctc tcgcgccgcg gggtcgatga
     3481 ccttctggta gccggggaag acgttgggca gggcccccat gtcgcaggcc ccctggacgt
     3541 tgttctggcc gcgcagcggg ttcacgcccg cggagggttt ccccatgttc ccgaggagaa
     3601 gctggagatt ggcggtgctc atcacgttgt ccttgcccgt ggtgtgctcc gtgatgccga
     3661 gcgtgtagca gagcatggcg ggttttatct cggagagcat gtgggcgacc ttccggatcg
     3721 tttccacatc cacgccggag atctcggcgg cccgttcggg cgggtactcc attatctttg
     3781 ccttgaaggc ctcgaagtta tcgcacctgt tctcgacgaa atccttgtca tacaggcctt
     3841 cgttgatgat gatgttcatg aggccgttga ggaaagcgat atcgctgccg accttgatct
     3901 ggcagtagat gtcggccagt ttcgcgagag gctgcttgcg ggggtcgacg acgacaagct
     3961 ttgctccctt gcgcagggcg ttcttcagga acgtggcagc caccggatgt gcctcggtca
     4021 tgttcgtgcc gatgcagagg aacatcttcg cgtcggcgaa ttcaccaaaa gaatttgtca
     4081 ttgctccgga accgaatgat tgcgccagac cggcgacggt tggcgcgtgt caggttcggg
     4141 cgcagtgatc gatgttattg gtgccgatca ccgcgcgaaa gagtttttgc atctggtagg
     4201 agtcttcatt tatgctacgg gcgcagctga cgccggctat ggcatcgggg ccgctctccg
     4261 ctattatccg tttgaatttg tcggccacga ggtcgagagc ctcatcccag gacgcctcgc
     4321 gaaaggaacc gttctcacgt atcaagggcg ttgtcagccg gtcttccgaa tagatgaaat
     4381 cgtagccgaa ccgtcccttc acgcagagcc ttcccttgtt gggtgctccg tcttccaccg
     4441 cggtcacctt gactatctgt tcccccttga ggtggagttc cagctggcag ccgactccgc
     4501 agtaaggaca tgtggtgcgt accttcctca tttcccaggc acgccccagg cctttggcct
     4561 ttctctccgt cagggctccc accgggcagg cctgcacgca ctgtccgcaa aagacacagt
     4621 tgctctcctg aagcggagtg tcataggcgg ttgcgacctt tgtgtcgaag ccgcgctgta
     4681 tggtctttat cgcctctata ccctggatct cattgcagac ccggacacac ttgaggcaga
     4741 ggatgcactt gttgtagtct ctcacgataa aagggttgga gtagtcgacg ggatagtcat
     4801 atttctcccc caccatcgcg gaacccgtca cccccagttc gtaggccagg ttctgaagcg
     4861 tacagtctcc cgtcttctcg catgtcatgc agtccagcgg atgacacgat accaggagct
     4921 cgacaataaa acgccgggca gccagaaccc gctcggtatc cgtcctgacc accatgcctt
     4981 ccgtggccgg ggccgaacag gacgccacaa ggttccgcat accctcgacc tccactacgc
     5041 agatacggca cgccccggtg ggggtcaggc ctgacagata gcacagcgtg gggatgtaaa
     5101 acccgttggc ccttgccact tcaaggactg tttgtccctc cgtggcctca caaacctttc
     5161 cattgattgt gattttcatc aaaacccctc cttcataacc ccgctattcg tgctattgga
     5221 gcacgtaggg ataaaaaaac cattcttttg tcattgatga tattattaga acacaggcaa
     5281 agcctggaag gccagtataa tccacaagaa atactgaaaa aaaaccgaat gtcacacaat
     5341 gatgcggtcc ggggatcacc ggggggcgaa ttgacaagcc tccaggcctt ctctagaata
     5401 agggtggcag ggatcgaagg tgaaccgaaa cgatgaccca cccggttgat cggcaggaaa
     5461 ggcagggacc aatggaagga ttcataaagt tcttcggcac cggcggcgcg cggttcgtgg
     5521 cgctcaaaca gcttcgggcc actggcggcc tgtggctcaa ttaccggaag acaaacctct
     5581 acatcgaccc cggtccgggt gccattgtcc gcatccgggc ggcgaaggag gattacgacc
     5641 cctcccgcct cgatgggatc gtcatcacgc acaggcacat ggaccacgcc aacgacgtca
     5701 acatccttat tgaagccatg gccgacggcg gcttcaggaa gagggggacc cttttttgcc
     5761 ccgcggacgc cctgtctggc gattccgtag tcttcgattt tgccggcaag tatctcgaga
     5821 acataatcct gcttcgggaa ggcggggagt acgccctggg cgacataact tttcgaacac
     5881 cagtcaggca caggcatccc
//
LOCUS       DAOQTR010000058         5871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_14994_length_5871_cov_3.067572, whole genome shotgun sequence.
ACCESSION   DAOQTR010000058 DAOQTR010000000
VERSION     DAOQTR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14994_length_5871_cov_3.067572"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1581
                     /locus_tag="PKN85_02315"
     CDS             70..1581
                     /locus_tag="PKN85_02315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT43263.1"
                     /translation="MTKKILGTKFAIQKRGGATGPAADTGAAEKMKKTDKLEQKIREL
                     EERNNDLLAMIENSYDAMAIADGESRLLLLNPAFERVMGIKNADIIGRKIEELVREGI
                     SDTGATLKVLETGKAQTVLINTIAGRQVLSTGIPVFDKHGRISKVYCNLRDITELNHL
                     KEKYKQSQKLVSKYLLELHEARRMQKAPSAFVAHSERMKQVIETAYRIARIDTTVLIL
                     GESGVGKDLVARLIHEASPRMETGTFVKINCGAIPPDLLESELFGHEAGAFTGARREG
                     KAGYFEIADKGTIFLDEIGDLPLRLQVKLLSVIQDQEVARVGGTKPKKVDARIIAATN
                     RDVEKMVKTGNFREDLYYRLNVVPFHIPALRERREEIPFLIKHYLNEFNSRYNLKVKL
                     TQEVVERLIAYRWPGNVRELANLVEQLVVLANEDLVTVEHLPKRFSAPERGDLAEMRE
                     LRPLDESVREFEIKLIRTALDQSKSNEEAAHKLGISLSTLARRLRQFKPDSHS"
     gene            1784..2929
                     /locus_tag="PKN85_02320"
     CDS             1784..2929
                     /locus_tag="PKN85_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT43264.1"
                     /translation="MKRFVFSVFVLMVFVALVAAGPAWSKEPIKIGGIFSLTGYLSWL
                     GEYKKKAAELKVEMINQAGGVNGQQLVLISYDDNSSPEQASKVAQRLISKDQVVAMAG
                     TASVPLSGAVASMANRYKIPTIINSGYAVDTSKDPYLFNTSHKTDFAVITAYKVFQKK
                     GIKKLALLMPIGPLGEIGGTAARKYAAEYGVTIVGEEKFDVKAADVTAQLAKIRNMNP
                     QAIFAFVTGEPAALIGRNMDQLSFKVPLLVSHGNANPGFLKLVSNVRTQMLVPSGKVM
                     KPEALPDSDPTKKVIVEFNKKHIAKYKEPANYFSAELADAIDLIAEGLRTSGTTDPVK
                     LRDAIENIKGFVGMQGIYTFTPKDHHGTRPDDMILLTVKAGKWEVPK"
     gene            2970..3836
                     /locus_tag="PKN85_02325"
     CDS             2970..3836
                     /locus_tag="PKN85_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT43265.1"
                     /translation="MSDISHLVQFLFSGITVGSVYALIAVSLVIVYKVSQLICFAQGE
                     FFVFGALTMTTLVSKGVPVPLAFFLSIIIAMALGALIQQVLIRPMKNTSVGALIVMTI
                     AISLALRGLALLIWGRESHVLPPFSRGEPIQVLGAALQLQILWIVGITTVVLFLIWFF
                     FEKTPAGIAMRACAENPYGASLMGINTQKTTLVAWAWGSGIGALAGMAVAPMLFTQYT
                     SGIMPMVKGFIAMSIGGLGSIVGSVIAGLLLGLVESYTIGLISSKFSDTIVFALLMAV
                     LVFRPNGLFSRS"
     gene            3846..4799
                     /locus_tag="PKN85_02330"
                     /pseudo
     CDS             3846..4799
                     /locus_tag="PKN85_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            4796..5599
                     /locus_tag="PKN85_02335"
     CDS             4796..5599
                     /locus_tag="PKN85_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT43266.1"
                     /translation="MMEKNGHILTISGVTKTFDGVRALDDVSFSVRSGDIKALIGPNG
                     AGKTTLLNAVSGLNPPTKGSVLFEGRDVVGLRTDQIASLGMSRTFQLIRLFTINNATV
                     LDNVLLGAHMHLRPTLLETVFLRGRIKRQEREATAKAMDLLKLVGIEKEAHALPGELS
                     FGSQRLLELARALMTGPRMLLLDEPASGLNDAEVEEFTRLLMTIKQQGITILIVEHNM
                     KLVMNIADDIIVLDFGRKLAEGPPSAICANPDVIEAYLGAQSKECGVTQ"
     gene            5596..>5871
                     /locus_tag="PKN85_02340"
     CDS             5596..>5871
                     /locus_tag="PKN85_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT43267.1"
                     /translation="MILKAERLSAGYARIQVLKAIDLEVKKGEIVCLVGANGAGKSTL
                     LKVLSGVIPPTAGAFTFDGRDVTGRKPNQLVRMGLSHVPEGRQIFGSL"
BASE COUNT         1295 a         1816 c         1645 g         1115 t
ORIGIN      
        1 gaatgatgcc gggcgtcatc catcgagaga tcagcaggat acccgcggca agccggggtg
       61 tggagtgcga tgacgaagaa gatcctgggt acgaaatttg caatccaaaa acgcggcggc
      121 gccacgggtc ctgccgccga caccggggct gcggaaaaga tgaaaaagac cgacaagctt
      181 gaacagaaga tccgcgagct cgaggaacgc aacaacgacc tcctcgcgat gatcgagaac
      241 tcctacgacg ccatggccat cgccgacggc gagagccggc tcctcctcct caatccggca
      301 ttcgagaggg tcatgggcat caagaacgcc gacatcatcg gccgcaagat cgaagagctc
      361 gtcagggaag gcatctcgga caccggggca acgctcaaag tcctggagac gggcaaagcc
      421 cagacggtgc tcatcaatac catcgccggg agacaggtgc tctccacggg catacccgtc
      481 ttcgacaagc acggcaggat ctccaaggtg tactgcaacc tgcgggacat caccgagctg
      541 aaccacctca aggaaaaata caagcagtcg cagaaactcg tgtcgaaata cctccttgag
      601 ctccacgagg cgagacgaat gcagaaggcc ccttcggcct tcgtggccca cagcgaacgg
      661 atgaagcagg ttatcgagac cgcctaccgg atagcccgga tcgacacgac ggtgctgatc
      721 ctcggtgagt cgggcgtggg caaagacctc gtcgcacgcc tcatccatga ggcctctccc
      781 cggatggaaa cgggcacctt cgtgaagatc aactgcggcg ccataccccc cgatctcctg
      841 gagtcggagc tcttcggcca cgaagccggc gctttcacgg gtgccaggag agaggggaaa
      901 gcgggatact tcgagatagc cgataaaggt acgatcttcc tcgacgagat cggagacctt
      961 cccctgcgcc tgcaggtgaa gctcctttcc gtcatccagg accaggaagt ggcacgcgtg
     1021 ggaggcacaa aacccaagaa ggtggacgcg aggataatag cggctacgaa cagggacgtg
     1081 gaaaagatgg tgaagaccgg caacttcagg gaagatctct attaccggct caacgtcgtt
     1141 cccttccaca taccggccct cagagagcgg cgggaagaga tacccttcct gatcaaacac
     1201 tatctcaacg agttcaacag cagatacaac ctgaaggtga aactgaccca ggaggtcgtc
     1261 gaacggttga tcgcttacag gtggcccggc aacgtccgcg agctcgccaa cctcgtggag
     1321 cagctcgtcg tgctcgccaa cgaagatctt gtgacggtcg agcaccttcc gaaaaggttc
     1381 agcgccccgg agcgcggcga ccttgctgaa atgcgggaat tgaggcccct tgacgagtcg
     1441 gtgagggaat ttgagatcaa gctcatcagg acggccctcg atcagtcaaa gtccaacgaa
     1501 gaggcggccc ataagctcgg gatcagcctc tccactctgg caagacgtct cagacagttc
     1561 aaacctgaca gtcattcctg acacgtcctc acaagcccaa gatcattgag cgaaacggac
     1621 cctgtcccct ggattgccat gtcaaaaatg acatgtcccc aggggtccgc gcgtttgcgg
     1681 gacatctcca aatccctatg agaccgaaat aacgaggttt tcagtgctgg cacccgactt
     1741 gcaatttgtc atttcgtaaa ccaacacttc aagggggtac ggaatgaaaa gattcgtttt
     1801 ttccgttttt gttctcatgg tctttgttgc cctggtcgct gccggtcccg catggtcgaa
     1861 agaacccatc aagatcggcg gaatcttttc cctgacggga tacctgtcct ggctcgggga
     1921 atacaagaag aaagccgcgg agctcaaggt cgagatgatc aaccaggcag gcggggtgaa
     1981 cggccagcag ctggtcctca tatcctatga cgataattcc tctcccgagc aggcatcgaa
     2041 agtggcccag aggctcatat ccaaggacca ggtggtagcc atggcgggga cggcctccgt
     2101 tccgctctca ggcgccgtgg catccatggc gaacaggtac aagataccta ccattatcaa
     2161 ctccggctac gccgtggaca cgagcaagga tccctatctc ttcaacacat cccacaagac
     2221 cgacttcgcc gtcatcacgg cctacaaggt gttccagaag aaaggcatca agaagctggc
     2281 gctgctcatg cccataggtc ctctcggcga gatcggtgga acagccgccc ggaaatacgc
     2341 ggccgagtat ggggtcacca tcgtgggtga ggagaagttc gacgtaaagg ccgccgatgt
     2401 aaccgcgcag ctggcaaaga tacgcaacat gaacccgcag gccatattcg ccttcgtcac
     2461 cggcgaaccg gcggccctca taggcaggaa catggaccag ctcagcttca aggtccccct
     2521 cctcgtctct catggaaacg cgaacccggg gttcctgaag ctcgtctcca acgtgcgcac
     2581 acagatgctc gttccgtcgg gaaaggtcat gaagcccgag gcgctgcccg atagcgaccc
     2641 caccaaaaag gtcatcgttg agttcaacaa gaaacatatc gcgaagtata aggaaccggc
     2701 aaactacttc tccgccgaac tggccgacgc catcgacctc atcgccgagg ggctgcgcac
     2761 ctccggtact accgatcccg tcaagcttcg cgacgccatc gaaaacatca agggctttgt
     2821 cgggatgcag ggcatctaca cctttacccc caaagaccat cacggcacca ggccggacga
     2881 catgatcctt cttaccgtca aggccgggaa atgggaagtg cctaagtaaa tgaaagagga
     2941 aaccggcagg gaccggtaag gagaaccacg tgagcgacat atcccacctc gtccagttcc
     3001 tcttcagcgg tataaccgtg ggcagtgtct acgcgctgat cgcggtgagc ctggtcatcg
     3061 tctacaaggt gtcccagctc atctgcttcg cgcagggaga gttcttcgtc ttcggggcgc
     3121 ttaccatgac caccctcgtt tcgaaggggg tcccggtccc tctcgccttc ttcctctcga
     3181 tcatcatagc aatggccctg ggagcactca tccagcaggt cctgatccgg ccgatgaaga
     3241 acacctcggt cggcgcgctc atcgtcatga ccattgccat ctcccttgcg ctgaggggtc
     3301 tcgcgctgct catatggggg agggaatcac atgtcctccc ccctttttcc cggggagaac
     3361 ccatccaggt actcggcgcc gccctgcagc tgcagatact ctggatcgtc ggaataacga
     3421 cggtcgttct ttttctcata tggttcttct tcgagaagac ccccgcgggc atagcaatgc
     3481 gcgcctgcgc ggagaacccc tacggcgcga gcctcatggg aatcaacacc cagaagacca
     3541 ccctcgtcgc ctgggcctgg ggttccggca tcggcgccct ggcgggaatg gccgtagccc
     3601 ccatgctgtt cacccagtac accagcggga tcatgcccat ggtcaagggc ttcattgcca
     3661 tgtccatagg gggcctcggg agcatcgtcg ggtccgtcat agcgggattg ctcctcggcc
     3721 ttgtggagtc atacacgata ggcctcatat cgtcaaagtt ctccgacacc atcgtcttcg
     3781 ccctgctcat ggcggtgctg gtgttcagac ccaacggcct cttcagcagg tcctaggaag
     3841 gtatgatgaa cgcgttgaaa cgttactggt ggctgatact gctggctgcg atagccgtct
     3901 atcccgtcat tggaaaaccg tcgccttaca ggagcggact cctcatcttc gcggggatct
     3961 atatcatcct cgccgtcggc ctggacctgc tcatgggcta cacggggcag atatcggtgg
     4021 gccacgcggc cttcttcgcc gtgggcgcct acacctcggg gatccttacc gcgaaatacg
     4081 gtttctcgcc cgcggccgcg atgggcgtcg gcgtcgtctt cagcgcgacg ctcgcgttcc
     4141 tcacgggccg cccggtgctc gcactgaagg aatactatct cgcgatggcc acccttgcct
     4201 tcaacgagat cgtggtcacc ctcatcatcg gttttgaagg gttgacgggt ggcgcgtcgg
     4261 gccttcgcga catacctccc ttcaccatct tcggcttctc gctcctcaac catcttcatt
     4321 attactactt tgtctggacc gtcgtggtcc tcgtcatcgc gagcagcatc gccgtggtcc
     4381 ggtccccctt cgggcgggcg ctcatcgcca tccattccga tgagcaggcc gcgcgctcct
     4441 tcggtgtgga ctgagcgaag tacaagaccc gcgcctacat gctcgccgcc gtctacgcat
     4501 cgatagccgg gtccctcttc gcccactaca tgggcttcat cgcccccgac gacttcggca
     4561 tactgacctc gataaatctc cttgtcatgc tctttctcgg aggcatcggg acgatattcg
     4621 gccccgccct gggggccctc ttcctcaagc tgctgcccga gatcacctat caattccagg
     4681 attacgagct cctcatcaac gggatcatcc ttatcgtggt gctcgtgttc ttccccaggg
     4741 gcctctttgg aatattcacg gccctcagga acaagatcgt tccacggatg ggcaaatgat
     4801 ggaaaagaac ggacacatac tgactatcag cggcgtgacc aagaccttcg acggggtcag
     4861 ggccctcgac gacgtgagct tcagcgtcag atcgggcgac atcaaggcgc tcatcggccc
     4921 caacggagcc ggcaagacaa ccctcctcaa tgccgtcagc gggctcaacc ccccgacaaa
     4981 gggctccgtc ctttttgaag gccgtgatgt cgtcgggtta aggaccgacc agatcgcttc
     5041 gctggggatg tcccgcacgt tccaactcat ccggctcttc acgatcaaca acgccaccgt
     5101 tctcgacaac gtccttctgg gggcccacat gcacttgagg ccgacgctcc tcgagacggt
     5161 cttcctgaga ggaaggataa aaaggcagga aagggaggcc acagctaagg ccatggacct
     5221 cctgaaactc gtcggcatcg aaaaagaggc ccacgcgctc cccggggagt tgtccttcgg
     5281 cagccagcgt ctcctggaac ttgcccgggc gctgatgact ggccccagga tgctccttct
     5341 cgatgagccc gcgtcggggc tcaacgacgc cgaggtggag gagttcacgc ggctcctcat
     5401 gaccatcaag caacagggta tcaccatcct catagtagaa cacaacatga aactggtcat
     5461 gaatatcgcc gacgacatca tcgtcctcga tttcggaagg aaactggcgg aagggccgcc
     5521 ctcggccatc tgtgctaacc ccgacgtcat tgaggcgtat ctgggcgcgc agagcaaaga
     5581 gtgcggggtg acgcaatgat cctcaaggcc gagagactat cagccggata tgcccgtata
     5641 caggtcctca aggcaataga cctggaggtg aagaagggtg agatcgtctg tctcgtgggg
     5701 gccaacgggg ctgggaaatc gacccttctc aaggtcctgt ccggcgtgat accacccacg
     5761 gcgggcgcgt tcaccttcga cgggcgggac gtgaccggac gcaagcccaa ccagctcgtg
     5821 aggatggggc tcagccatgt ccccgaggga cgccagatct tcggctccct t
//
LOCUS       DAOQTR010000059         5776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_15324_length_5776_cov_3.558294, whole genome shotgun sequence.
ACCESSION   DAOQTR010000059 DAOQTR010000000
VERSION     DAOQTR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15324_length_5776_cov_3.558294"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /gene="sppA"
                     /locus_tag="PKN85_02345"
     CDS             <1..553
                     /gene="sppA"
                     /locus_tag="PKN85_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNT43268.1"
                     /translation="MGSVCASGGYYIAAVGEKLYANPSTITGSIGVIMQQTVVEELMK
                     KIGVQSNTIKAGAMKDVGSPFRKMTDNERQYLQDIINNIHEQFIKDVAAGRKMPLDKV
                     RSLSDGRIYTGLQAKEAGLIDGIGTFYDVVDDMKKAVGITGKPELVYGKRPFAPLKWL
                     FSSMAREMLGREQADPFQYKYAP"
     gene            739..1458
                     /locus_tag="PKN85_02350"
     CDS             739..1458
                     /locus_tag="PKN85_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNT43269.1"
                     /translation="MDIYERLKDELRLLAGDSMDEKVSVVSARALTAKEAIGETGRDD
                     YPLLKGKEAMVEALYKGVKGHAFTDMPGGFEGTVRDVIMLPLANNFERAVFIATINAV
                     MREAGLITGTVHCRDDEPGRCARELVSYVREKFGNPRIAFVGFQPGMIERLSEVFQMR
                     VLDLDEDNIGKKKFGIVIESPEATEDVLAWGDIILATGSTCVNGTITRFVGQKPVIFF
                     GVSGAAVTKLMGFERYCRYPH"
     gene            1904..2299
                     /locus_tag="PKN85_02355"
     CDS             1904..2299
                     /locus_tag="PKN85_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43270.1"
                     /translation="MFSGSGERGGRGPGAILSGIADIVQLAEDYLNEAVDASHGYDPQ
                     VVSQIRADLASLDAFLARLTEVRTMRDDTLFRKAAVELRSQVPALQAVSDSVKRTASD
                     TATPPGVGGYMEQTVTLLAQAVELLRELP"
     gene            2338..2586
                     /locus_tag="PKN85_02360"
     CDS             2338..2586
                     /locus_tag="PKN85_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43271.1"
                     /translation="MAIFRVTYRNALNSEGEPFELPADKLDTNGARIDCYGYVEREIR
                     EGDNFSRDVYEYDIPDNDADRFIEGLKRTPNVMDYKRQ"
     gene            complement(2685..4364)
                     /locus_tag="PKN85_02365"
     CDS             complement(2685..4364)
                     /locus_tag="PKN85_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNT43272.1"
                     /translation="MPIFRARETYRDLKRYSQIASVLAHHGFTEVLDRLKARYRAPRK
                     RKPLIKERVAEGPPIPVRLRLVFEELGPTFIKLGQILSSRPDILPPDYIKELSKLQDD
                     VPPFPFEEARTLLETQLRHPLRELFSSFEEKPVAAASLAQVHRARTPAGEEVAVKIQR
                     IGIEEVIETDIRILREFAGLAVRHVPESRYFGPLELVEGFARTIRHELDFVREGRNID
                     RFKAHFISTPTVYIPKVHWDLTSSKVLTIEFITGIKISDVDRLTAAGLDRKTIALNGA
                     NLTLEEIFELRRFHADPHPGNIFVLDNNVIAPVDFGMTGTIDEETSEQMTGIFVAVMQ
                     KDVDTLVGLLRTIGIVGEPYDDRAFKRELHDLFDRYYGVPLNRLDIGTITRELMTIVH
                     RHKLRLPSEFAMIGKALLISEGVGRLLDPEFNIIETARPYARRILLRRFDPARQLRGL
                     SRVADEVIALLRALPADTREILTMMRKGKLAMRFEHRGLELLTDELDRSSNRISFALV
                     IAALIIGSSLIFQTGVGPTVFGYPVLGLGGFLLASILGVWLLIGIMRSKRL"
     gene            complement(4368..4676)
                     /locus_tag="PKN85_02370"
     CDS             complement(4368..4676)
                     /locus_tag="PKN85_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43273.1"
                     /translation="MIRKAVFMGLGAISMTKDKAEELVDDLIKRGEVASAERFKTVDK
                     LLKEADKQEKEFQHKVSAAVQKVIVEMGLPTRKDLEEIGKTLKKIEAKISSTEKKDAG
                     "
     gene            4964..5644
                     /locus_tag="PKN85_02375"
     CDS             4964..5644
                     /locus_tag="PKN85_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNT43274.1"
                     /translation="MENNGTAWVDLSISPAYRSRYPGVAFGSTLISDCLPLSDAAGFD
                     QHRRGLLRKMRKRETLAGISTRIDLYDRFFRSFGFECPLPGHLKRTVLSGFPRYNLMV
                     DAHFMAEMCAGILVAVTDHDRIEGRLTLDVARRGEVCAGMGGRRFTTIEGEIVLRDER
                     DIICVLCQGADEKTRVRDDTRNVLFYSYAVPGVEGVYLREGLSVAAEVMSRFGGGNVK
                     GLMVFEGT"
BASE COUNT         1212 a         1620 c         1759 g         1185 t
ORIGIN      
        1 gatggggtcc gtctgcgctt caggaggtta ctacatcgcc gctgtcggcg aaaagttgta
       61 cgccaacccg tccaccatca ccggttccat cggtgtcatc atgcagcaga ccgtcgtgga
      121 agaactgatg aagaagatag gagtgcagag caatacgatc aaggcggggg cgatgaagga
      181 cgtgggaagc cccttccgga agatgacaga caacgagcgc cagtacctcc aggatattat
      241 caacaacatt cacgagcagt tcatcaagga cgtggccgcc ggacggaaaa tgcccctcga
      301 caaggtgcgg agcctctccg acggcaggat atacacgggc ctccaggcga aggaagcggg
      361 tctcatagat ggaataggca ccttctacga cgtcgtcgat gatatgaaga aggccgtcgg
      421 cattacgggc aaaccggaac tcgtctacgg gaaaaggcct ttcgcgcccc tcaagtggct
      481 cttctcctcc atggcgcgcg aaatgctcgg ccgcgaacag gcggaccctt tccagtacaa
      541 gtacgcaccc tgaagacggt tgcaaccgct taaaccgctt gagccgctta aacgttcgag
      601 acggttggat gttgtctctt aacgttcaag cggttgcagc ggtctttctc ttctccccgt
      661 tgacaatcca cctccaatcc ggctatatta cgctagctaa caagctatac aacgaactgt
      721 aaagggggtg actctttggt ggacatttat gaaaggctta aggacgaact caggctcctg
      781 gccggtgact cgatggacga aaaggtgagc gtggtttcgg cccgggcgtt gaccgcgaaa
      841 gaggcgatag gtgaaacggg gcgcgatgat tatccgcttc tgaagggcaa ggaggcgatg
      901 gtggaggctc tttacaaggg tgtaaaggga cacgccttta ccgatatgcc gggcggtttc
      961 gaggggaccg tgcgcgacgt cataatgctg cctctcgcga acaacttcga gcgggccgtc
     1021 tttatcgcca ccatcaacgc ggtcatgcgc gaggccggac tgataacggg gaccgtccac
     1081 tgccgggacg acgaaccggg acgatgtgcc cgggagcttg tttcctacgt gagagagaag
     1141 ttcgggaacc cccgcatcgc cttcgtcggc ttccagcccg gcatgatcga gcggctctcc
     1201 gaggtcttcc agatgcgggt cctcgacctc gacgaagata acatcgggaa gaagaagttc
     1261 ggcatcgtca tcgagagtcc cgaggccact gaagatgtcc ttgcctgggg cgacatcatt
     1321 ctggcaacgg gttcgacctg tgtcaacggg acgatcaccc ggttcgtcgg tcaaaagccc
     1381 gtcattttct tcggggtctc cggagcggct gtcacgaagc tgatgggttt tgaacgttat
     1441 tgccggtatc ctcattgagc ggcgcagggc cgccggaacc atagctgagc ggcgacgacc
     1501 ccgggcttca cgagaggcct gtcaggaggt ctttcccgtt cgcgagcacc gttaccgccg
     1561 ttccgtcgaa gtggagcgat atgtcattcg tcccatgttg ttcgaagagg acggcatacc
     1621 ttgagacaac gcttccggga tgtgcactgg aggcgttttc tttctgaagc gtgaccgtcc
     1681 ggaacgcggg tggacggagg ccttctggtg aatatcgatg tgcccgtgta tgatggcgcg
     1741 acgggcacgg ccgtcgcgga cccttgacgg ccgtcgatca gtctttttca atcccccgca
     1801 tcgactccgg caacctccgc tccttgcaat gaggaggtcg gagggttaat attttagtat
     1861 agcgcgggta ccgcggaaac gatcactgaa gaggaggaat gccatgttct ctggatcagg
     1921 cgaaaggggc ggccgtggtc ccggggcgat cctgtcgggg atcgccgata tcgttcagct
     1981 ggcggaggat tacctcaacg aggccgtcga tgccagtcat gggtatgatc ctcaggtcgt
     2041 cagccagatc cgcgccgacc tcgcgtccct cgacgccttc ctggcgcgcc tgaccgaggt
     2101 gcggacgatg cgtgacgata cccttttcag gaaggccgcg gtggagttga gatcgcaggt
     2161 cccggcgctc caggcggtaa gcgatagcgt caagagaacg gcgagcgaca ccgccactcc
     2221 gcccggcgtc ggaggctaca tggaacaaac cgtgaccctt cttgcccagg cggtggaact
     2281 tctgagggag ttgccatagg ggcgggcggt cattacgggt aaggagggaa ggttcagatg
     2341 gccatattca gggtaacata caggaacgct ctcaacagcg agggtgaacc cttcgaactc
     2401 cccgccgaca aactcgacac gaacggcgcg aggatcgact gctacggata cgtggaacgg
     2461 gagatcaggg aaggcgacaa cttttcccgt gatgtttacg aatacgacat ccccgacaac
     2521 gacgccgacc gtttcatcga gggtctcaag agaacaccca acgtcatgga ctacaaaaga
     2581 caatagcggg agcgatcccg gtcttctgaa gggaccttga gacccggcgt acgtacccgc
     2641 cgaccgtcaa gcgactcaag caattcaagc ccctcaagcc gccttcaaag cctctttgac
     2701 ctcatgatgc cgatgagcag ccacaccccg aggatgctgg cgaggagaaa gccgccgagg
     2761 cccagaacgg ggtaaccgaa gacggtcgga cccacccctg tttggaagat gagagaggac
     2821 ccgataatga gggccgctat caccaaagca aaactgatgc ggttgctcga ccggtcgagt
     2881 tcgtcagtga gaagctccag cccccgatgt tcgaagcgca tggccagctt ccccttccgc
     2941 atcatggtga gtatctctct cgtgtccgcc ggcaaagccc tcaataacgc gatgacctcg
     3001 tcggcaaccc tggaaagacc gcgcaactgc ctcgccgggt cgaatctgcg caagaggatc
     3061 cttctcgcat agggccgcgc cgtctcgatg atgttgaatt cagggtcgag aagcctcccg
     3121 acgccctcgc tgatgagaag cgccttgcct atcatcgcaa attccgacgg tagcctcaat
     3181 ttgtggcggt gaacgatggt catgagttcc ctggtgatgg tcccgatatc gagcctgttc
     3241 agaggcacgc cataataacg gtcgaagagg tcgtgcagct cacgtttaaa ggcgcggtca
     3301 tcatacggtt ctccgacgat gccgatcgtt cgcaacagcc cgacgagagt gtccacatcc
     3361 ttctgcatga cggccacgaa tatgccggtc atctgctcgg aggtctcttc atcgatggtc
     3421 cccgtcatgc cgaagtcaac gggcgctatc acgttgttgt cgaggacgaa gatgttgccg
     3481 gggtgaggat cggcgtgaaa acgacggagc tcgaatatct cctcgagggt gagattggcc
     3541 ccgttcaggg caatggtctt cctgtcgagg ccggcggccg tgagacggtc gacgtcggat
     3601 atctttattc ccgtgatgaa ctctatcgtc agaaccttgg acgacgtcag gtcccagtgc
     3661 acttttggaa tatagaccgt cggcgtactg atgaaatggg ccttgaagcg gtcgatgttc
     3721 cgtccttccc tgacgaaatc gagttcgtgc cggatggtcc tggcaaaacc ttccacaagc
     3781 tccagggggc cgaaataccg gctctccggg acgtgccgca cggcgaggcc ggcgaactcc
     3841 ctgagtatgc ggatgtcggt ctcgatgacc tcctctatac cgatccgctg gatcttgacc
     3901 gccacttctt cacctgccgg tgtcctggca cggtggacct gcgcgaggga agccgccgcg
     3961 accggtttct cttcgaagga tgaaaaaagc tcacggagcg ggtgcctcaa ctgcgtctcc
     4021 aggagagttc tggcttcctc gaaagggaag ggagggacat catcctgaag cttgctcaac
     4081 tcctttatgt agtcgggcgg cagtatatcg gggcggctgc tcagtatctg acccagcttt
     4141 atgaaggtgg gacccagttc ctcgaagacg agacggagcc gcaccgggat gggaggtcct
     4201 tcggcaacac gctctttgat gagcggtttg cgcttccgtg gagcgcggta tcgcgccttc
     4261 agtctgtcca ggacctccgt gaagccatgg tgggcaagga cagaggcgat ctggctgtac
     4321 cgcttcaggt cccggtacgt ttcacgagct cgaaagatgg gcacctgcta acctgcatcc
     4381 ttcttttccg tggatgatat cttcgcctcg atcttcttca acgtcttgcc gatctcctcc
     4441 aggtctttgc gcgtgggaag acccatctcg acgatcacct tctgtacggc cgcggacacc
     4501 ttgtgctgaa attccttctc ctgtttgtcc gcttccttga gaagcttgtc gacggtcttg
     4561 aacctctccg cgcttgccac ttcgccgcgc ttgatgagat cgtcgaccaa ctcttcggct
     4621 ttgtcctttg tcatgctgat ggcgccaagt cccatgaaca ctgctttgcg tatcatatcc
     4681 ataatacccc ctttgctgaa ggcgccgccg gctggaaacg ccccttcttc tgcgggaact
     4741 gcggaatctc cggtcagcgc gcgcggctcc tcagcttaaa gaatatatta ggccaccgaa
     4801 cggccgattt caagtcacgg aacgtgacgc tccaggtcat tgcgtctgac cgatcagcgg
     4861 atctttgtgg taagtggtcg aaaacagggt ataatgtagg cacgttccgg gcgtgacaat
     4921 tcgttgtcga tgggaaggga tcgaagacca taggaggtgg gaagtggaaa acaacgggac
     4981 ggcgtgggtg gacctttcga tctcccccgc ttatcggagc aggtacccgg gtgtcgcttt
     5041 cggatcgacg ctcatatccg actgcctgcc tttgagcgac gccgccgggt ttgaccagca
     5101 caggcggggt ctcctcagaa aaatgagaaa gcgcgagaca cttgccggca tatcgacgcg
     5161 catcgacctc tacgacagat tcttccggag cttcggcttt gagtgccccc tgcccgggca
     5221 cctcaagaga acggtcttga gcggcttccc gagatacaac ctgatggtgg acgctcactt
     5281 catggcggag atgtgcgccg gcatcctcgt ggcagtgacg gaccacgatc gtatcgaagg
     5341 gaggctcacg ctcgatgtgg cccggcgcgg ggaggtgtgc gccggaatgg gaggccgccg
     5401 gttcacgacg atcgaggggg agatcgtgct ccgggatgaa agggacatca tctgtgtcct
     5461 ttgccagggg gcggatgaga agacaagggt tcgggacgat acgcgcaacg ttcttttcta
     5521 ttcctatgcg gtgcccggcg tagagggggt ctatctcagg gaaggactga gcgtggccgc
     5581 ggaggtcatg tcccgctttg gtggcgggaa cgtgaagggc cttatggtgt tcgaagggac
     5641 ctgagatggg cttccccagg gagccctgat gggacaggac atcgtcgaga cctgctcccg
     5701 gaccgggtcc tcggggtcgg aaaagaacta aacttatcag catatccgcc gataacagga
     5761 gtaagggtgt atgccg
//
LOCUS       DAOQTR010000060         5770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_15340_length_5770_cov_3.693963, whole genome shotgun sequence.
ACCESSION   DAOQTR010000060 DAOQTR010000000
VERSION     DAOQTR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15340_length_5770_cov_3.693963"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKN85_02380"
     CDS             complement(<1..474)
                     /locus_tag="PKN85_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43275.1"
                     /translation="MFPEAFKYSVAKSVNSAIKWLLNTHGSAFDSFSSGILSLLLWVQ
                     GILVSIPWWAYIAFIALMAWIATRKIIPVIVLAVLPIFIGMFGLWHLALETLALVITS
                     VVIALAIGIPMGVLMAEARYFAEFIKPVLDAMQTMPSFVYLIPAMMLFGLGKVPAV"
     gene            complement(464..1348)
                     /locus_tag="PKN85_02385"
     CDS             complement(464..1348)
                     /locus_tag="PKN85_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HNT43276.1"
                     /translation="MAENVIDVKELWKVYSKKDIDLNVEDEEMVESLDKSSDSVVAVR
                     DVTFSVKPGEVFIVMGLSGSGKSTLIRCLLRLVDISWGSVTINGQDVTAMNKKELTEF
                     RRNQAAMVFQHYGLLPHRTVIENVAFGLKLRGIPKKERRERARETLNIVGLEQWGDYY
                     PASLSGGMRQRVGIARALVADTPLLLMDEPFSGLDPLIRREMQDELVRIQEELHKTIF
                     FVTHDLDEAMRLGDRMAVMRNGEIVQIGRPPEVFANPADDYVARFVQDKRDEIRKTDE
                     QMAALAAQSEKAGTPDVS"
     gene            complement(1355..2365)
                     /locus_tag="PKN85_02390"
     CDS             complement(1355..2365)
                     /locus_tag="PKN85_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT43277.1"
                     /translation="MKAGRKTFLILALALVVGLTSIPAASAAGNVVMVDFSWSSVQMH
                     NRVAGFILEHGYGFSPDYMFAESVPGLTGLQQGDIDVAMEMWIDNVLEWYTDAVEENK
                     QVLDLGSVFPDAPQGWYVPTYMIKGDKERGIEPMAPNLKSVFDLPQYWELFKDRETPD
                     KGRFYNAIPGWVVHDINLKKLEAYNLTETFINFDPGSQTALMTAIVSAYEKGEPVLAY
                     YWEPEWIMGMLDMTLLEEPEYDEKLWNEENKFACAFPAARVHVGINAEFAKKNPEILA
                     FLEKYETTLAQNNQALAYMQQEEATVEEAAIWFLKNYKEVWKAWLPGDVAGKVEAALA
                     GK"
     gene            2752..3450
                     /locus_tag="PKN85_02395"
     CDS             2752..3450
                     /locus_tag="PKN85_02395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNT43278.1"
                     /translation="MIEKRLRHTRIYEQVVNELKELIASGELLPGDPLPPERQLMSEM
                     GVSRSSLREAFRVMELLGLIDSVPGKGRFVRKPRGETADSRNMPLEDEAVLELMEARR
                     ALDPAIAGAAAMHAMPSDLMKLRKVLSRTEKNLEHIEKRSQDDFDFHLMLAEATHNFV
                     FINIVKMTFNLIMATHERIYSLLADKEAFFNEHQTIYEAIIDHDVARAQSLSAGHIER
                     IYRTLQEGLALEVK"
     gene            3497..4051
                     /locus_tag="PKN85_02400"
     CDS             3497..4051
                     /locus_tag="PKN85_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT43279.1"
                     /translation="MRDRIVKMIPEIDWIQDKELREKVVATIEDALKTGGWEPEDMDK
                     IPFTLLIPDCPASLNVHNRGVTRMAKVIYEEYNPLYKDNGGFELDHDTLIAGAILHDV
                     GKLVEYEKKDGKAVKSQMGKDLRHPFSGVGLAMRNGIPSRICHCIAVHAKEGDGGYRC
                     PEAVVINKCDFINFETIKSFLGLL"
     gene            4085..5365
                     /locus_tag="PKN85_02405"
     CDS             4085..5365
                     /locus_tag="PKN85_02405"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HNT43280.1"
                     /translation="MGKTMIEKIIRRAAGKEVKVGERVWCNIDLSTARDFGGANCVLQ
                     FEEVTDKKGKVWDPDKVAFTFDLQAPSHSEKVSTNQKIIREFAKKQGIKKVFDVDWGI
                     GQHVLLENGLVKPGDVILGTDSHMNLLGAVGAFATGVGNTDIVASWFEGTNWFRVPET
                     MKITVNGKFAKGVCMRDLLTHIVGTLGADGMFFLSTEFYGDTIERSSLSDRITLCSMV
                     TEMSGKIGLIMPNGKVLDWLVERAGKEVVDRVKSLSADPDAVYCQELEFNVDNLEPMT
                     SCPDAPDNVKKVREVAGEHVDQVHIGSCSNGRFEDISAAFEVLKASKSKVAPGMRAII
                     TPATREVMLQCAEAGYIQKFLEAGVIFTNPTCALCTAEHYGALPSGDVGMSTTNRNFI
                     GKVGKGSHTYLASPMTAMATAIRGKITDPRDILG"
     gene            5380..>5770
                     /locus_tag="PKN85_02410"
     CDS             5380..>5770
                     /locus_tag="PKN85_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNT43281.1"
                     /translation="MENKVLRGKAWVFGDDIDTDLIYHNKYLAETDPRKMPQYSFEYL
                     PGKENFAKEVKEGDFVVAGTNFGCGSSREHAVYCLKYAGVPVILAESFSRIYYRNAIN
                     NGYPVLFVKGLSQAIKEGKIKDGDDLEV"
BASE COUNT         1367 a         1608 c         1632 g         1163 t
ORIGIN      
        1 aacggccggg accttcccca ggccgaagag catcatcgcc gggatcaggt atacgaagct
       61 gggcatggtc tgcatggcgt ccagcacggg cttgatgaat tctgcgaagt atctcgcctc
      121 agccatgaga acgcccatgg gtatcccgat ggcaagggct atcaccaccg atgttattac
      181 cagggccagc gtctcgagcg ccaggtgcca gagtccgaac atgccgataa agatcgggag
      241 caccgcgaga acaataacag gaatgatctt ccttgtggct atccaggcca tgagggcgat
      301 gaaggctatg taggcccacc aggggattga gacaagaatg ccctgcaccc acagcagaag
      361 actcagtatc ccggatgaga aactgtcgaa ggccgatccg tgggtgttca gcagccattt
      421 aatggcgcta ttaacgctct tcgcaactga gtatttgaag gcttcaggaa acatcagggg
      481 tccccgcctt ttcagactgc gcagccagcg cggccatctg ttcgtcggtc ttccggatct
      541 cgtcacgctt gtcctgcacg aacctcgcga cataatcgtc ggccggattg gcaaagacct
      601 caggaggtct gcctatctgg actatctcgc cgtttcgcat taccgccatg cggtcaccaa
      661 ggcgcatggc ctcgtccagg tcatgggtaa cgaagaagat cgtcttgtgt agttcctcct
      721 ggatccggac cagttcatcc tgcatctcgc gccggatcag gggatcgaga ccgctgaagg
      781 gctcatccat aagcaaaagg ggtgtgtccg ccaccagggc ccgggctatc ccaaccctct
      841 ggcgcatccc tccgctcaag gaagcaggat agtaatcacc ccactgctcc agccccacga
      901 tattgagcgt ctcgcgagcc ctctccctgc gctccttctt tggaatcccc cgaagcttca
      961 ggccaaaggc aacgttctcg atgacagtcc ggtgagggag gagtccgtag tgctgaaaga
     1021 ccatggctgc ctggttgcgt cggaattccg tcagttcctt cttgttcatg gccgttacgt
     1081 cctggccgtt gatcgtcacg ctgccccagg aaatgtcgac aagcctaaga agacagcgta
     1141 tcagcgttga ttttccgctg cccgagagac ccatgacaat aaagacctcg ccgggtttaa
     1201 cggagaaggt tacatccctt acggcgacga ccgagtcgct ggacttgtcc agcgactcga
     1261 ccatctcttc gtcttcgacg ttgagatcga tatccttttt cgaatagacc ttccagagtt
     1321 cctttacatc aataacgttc tccgccatgg gcgtctactt gcctgcaagg gctgcctcga
     1381 ctttcccggc cacatcaccg gggagccatg ccttccagac ttccttgtag tttttgagga
     1441 accagatagc cgcttcctcg acagtggctt cctcctgctg catgtaggca agggcttggt
     1501 tgttctgggc aagggtggtc tcgtattttt cgaggaaagc aaggatctct gggttctttt
     1561 tggcaaattc ggcgttgatc ccaacatgga ccctggctgc ggggaaagcg catgcgaact
     1621 tgttctcctc gttccagagc ttttcgtcgt attcgggttc ctccagcagg gtcatgtcca
     1681 gcatgcccat gatccactcc ggctcccagt agtaggcaag gacaggttcg cccttctcat
     1741 aggcagagac tatggcagtc ataagcgctg tctgggaacc cggatcgaag ttgatgaatg
     1801 tttcggtgag gttgtaagcc tccagtttct tcaggttgat gtcgtgtacg acccatccgg
     1861 ggatagcgtt gtagaaccgc cccttgtctg gggtctcgcg atccttgaag agctcccagt
     1921 actggggaag gtcaaagacc gacttcaggt tcggggccat gggctcgata ccgcgttcct
     1981 tgtcaccctt gatcatgtag gtgggtacat accatccctg aggggcatca gggaaaaccg
     2041 acccaaggtc aagaacctgt ttgttttcct caacggcatc ggtgtaccac tcgagaacgt
     2101 tgtctatcca catctccatg gctacatcga tgtcgccctg ctgaaggcct gtgagccctg
     2161 gaacggattc agcgaacatg taatccggcg agaagccgta accatgttcc aggatgaaac
     2221 cggcaacccg gttatgcatc tggacactgc tccagctgaa atcgaccatg actacattcc
     2281 cggctgctga tgcggcgggg atcgaggtca gccccaccac aagggcaagg gcaaggatca
     2341 gaaaagtttt tcgtcctgct ttcatgacaa aatccctcct ttggatttac acatgcagaa
     2401 acagaagatg gggggaaatc ctcgaattgt tccgacccca cgagggtcgt caaaggtacc
     2461 gtttccgcgg gactcgcgga gaacagaaga caggaactcc acttgaaaaa cgacagaatg
     2521 atgcttggga agagttgtga aatgttgtcc gacacaacat ttcacattga atagccggta
     2581 cctatacccc ccggaccaag ttatgttagt tgaatccggg gaagttgtca aacgtttgta
     2641 tgcaaaataa agtttcaaat tgcctgaaca tttagtctta ttgcgaacac ttgcacaaag
     2701 ggtctacctg gtatataaag tgtactgggt ggtccaccga ggagggaaga tatgatcgaa
     2761 aaaagacttc gccacacaag gatatacgaa caggtggtca acgagctaaa ggagctgatc
     2821 gcatcgggcg aactgctgcc gggagacccg ctccccccgg agcggcagct catgtcggag
     2881 atgggtgtga gcagaagctc cctgagagag gccttcaggg tcatggagct gctggggctt
     2941 atcgacagcg tccctggcaa ggggcgtttt gtccgaaaac cccggggaga gacggccgat
     3001 tccaggaaca tgccgctgga ggacgaggcc gtgctggagc ttatggaggc ccggagagcc
     3061 ctggacccgg caattgccgg agcggcggcg atgcatgcca tgccttcgga cctgatgaag
     3121 ctgcgcaagg ttctctccag gaccgagaaa aacctggagc acatcgaaaa gagatcccag
     3181 gacgatttcg atttccacct gatgctggca gaggctaccc acaatttcgt gttcatcaat
     3241 atagtgaaga tgaccttcaa cctgatcatg gccacccacg aaaggatcta ctccctgctt
     3301 gcagacaagg aggccttctt caatgaacac cagaccattt acgaggctat catcgatcat
     3361 gacgtggcaa gggcccaatc gctttcggcg gggcacatag agaggatcta caggacttta
     3421 caggaagggc tggcccttga ggtcaagtaa atagatagag gcgcttgagt ctcaaaaacc
     3481 attggaggga tggaacatga gagacaggat cgtgaagatg atccccgaga tcgactggat
     3541 ccaggacaag gagctcaggg aaaaggtcgt agcaaccatc gaggatgcgc tcaagaccgg
     3601 cggatgggag ccggaagata tggacaagat ccccttcaca ctgctcatcc ccgactgccc
     3661 agcctctctc aacgtccaca acaggggcgt gacgagaatg gcaaaggtta tctacgagga
     3721 gtacaacccc ctctacaagg acaacggcgg gttcgagctt gaccatgaca cacttatcgc
     3781 cggcgcgatc ctccatgacg ttggcaaact ggtggagtac gaaaagaagg acggcaaggc
     3841 agtgaagtcc cagatgggca aggatctgcg ccaccccttc tccggtgtcg gcctggccat
     3901 gcgtaacggc ataccctcga ggatctgtca ctgcatagcc gttcacgcca aggagggcga
     3961 cggcgggtat cgctgcccgg aggcagtggt catcaacaag tgtgatttca tcaacttcga
     4021 gaccatcaag tcgttcctcg ggcttctcta gcaggaacga tccaaaacag gaggtgcgtg
     4081 acccatgggc aaaacgatga tcgagaagat catccggcgg gcagccggca aggaggtcaa
     4141 ggtcggagag agggtctggt gcaatatcga cctctcgacg gcccgcgact tcggcggcgc
     4201 caactgcgtt ctgcagttcg aagaagtcac cgacaagaag ggcaaggtat gggatcccga
     4261 caaggtggcc ttcaccttcg accttcaggc tccatcccat tcggagaagg tctcgacaaa
     4321 ccagaagatc atccgggagt tcgcaaagaa gcagggcatc aagaaggtct tcgacgtgga
     4381 ctggggtatc ggacagcatg ttcttcttga gaacggcctt gtaaaacccg gcgacgtcat
     4441 cctcgggaca gacagccaca tgaacctcct cggggcggtg ggcgccttcg ccaccggcgt
     4501 tgggaatacc gacatcgtgg catcgtggtt cgaggggacc aactggttcc gcgtgcccga
     4561 gacgatgaag atcaccgtca acggcaagtt cgcaaagggc gtctgcatgc gcgacctgct
     4621 gacgcacatc gtggggactc tcggcgccga cggaatgttc ttcctgtcca ccgagttcta
     4681 cggcgacaca atcgagaggt cgtccctttc ggacaggata accctctgct ccatggtcac
     4741 cgaaatgagt ggcaagatag ggctcataat gcccaacggc aaggtcctcg actggctggt
     4801 tgagagggcc ggcaaggagg tcgtggacag ggtcaagagc ctttcagcag acccggacgc
     4861 cgtctactgc caggagctgg agttcaacgt ggacaacctt gaacccatga catcctgccc
     4921 agacgcgccg gacaacgtca agaaggtccg cgaggtcgcc ggcgagcacg ttgaccaggt
     4981 gcacatcggc tcctgctcca acggccgttt cgaggacatc tcggcagcat tcgaagtcct
     5041 caaggcttca aaatccaaag tggctcccgg aatgcgcgcc atcattaccc cggctacccg
     5101 ggaagtaatg cttcagtgcg ccgaggccgg ctacatccag aagttcctcg aggccggggt
     5161 catctttacc aacccgacct gcgcgctatg caccgccgag cactacgggg ctctcccgag
     5221 cggcgacgtg ggcatgtcga ccaccaacag gaactttatc ggcaaggttg gcaagggaag
     5281 ccatacttac ctggcaagcc cgatgaccgc aatggccacg gccattcgcg gaaagataac
     5341 tgatcccagg gatatcctcg gatagggagg ggacgactga tggaaaataa agtccttcgc
     5401 ggaaaggcct gggtctttgg tgatgacatc gacaccgacc tcatctacca caacaagtac
     5461 ctggccgaga cagatcccag gaaaatgccg cagtactcct tcgagtacct gcccggcaag
     5521 gagaatttcg caaaagaagt caaggaaggc gattttgtcg tcgccgggac aaacttcgga
     5581 tgcggttcat cccgcgagca cgccgtctac tgcctcaagt acgccggagt accggtgatc
     5641 ctggccgaat ccttctcgcg gatctactac cgcaacgcca tcaacaacgg atacccggtc
     5701 ctattcgtca aggggctcag ccaggcgatc aaggaaggga agatcaagga cggcgacgat
     5761 ctcgaggtcg
//
LOCUS       DAOQTR010000061         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_15547_length_5704_cov_3.228536, whole genome shotgun sequence.
ACCESSION   DAOQTR010000061 DAOQTR010000000
VERSION     DAOQTR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15547_length_5704_cov_3.228536"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKN85_02415"
     CDS             complement(<1..328)
                     /locus_tag="PKN85_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT43282.1"
                     /translation="MVPAKLDYLKGPMYTELHVHSNFSFLDGASSTRSLVERAASLGM
                     GALAITDHDGLYNACIFSKEAREAGIRPIIGAELTLEGDHHLTLLIENARGYANLSQL
                     ITRAHLA"
     gene            complement(301..552)
                     /locus_tag="PKN85_02420"
     CDS             complement(301..552)
                     /locus_tag="PKN85_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43283.1"
                     /translation="MKTSPFDPEPVEVIAVSGRPVQVTFRKKRQKVRDIVNIWRVDDT
                     WWQRPVVRMYYTLELESGRRVTVFQDLPGGAWYRQNWTI"
     gene            complement(549..1718)
                     /locus_tag="PKN85_02425"
     CDS             complement(549..1718)
                     /locus_tag="PKN85_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43284.1"
                     /translation="MPHFHVQVECLKDPDLGKRPVVIVGMPEERGFVIDCSEELAHRG
                     VAPMMPLRDVYHLCYDAVPVLARRREHSLLWEEVLSSIAGITLRMEPKKEGTVFLDVT
                     RLPGMYKSEEQVASALGSMVSCQFRLDVRIGAGNSRFMAFEAALCASKDARVIPSGME
                     REFLAPLGIDRLPVPDDVRERLHLLGLHSLGQISAFTLSALTSQFGAAGKELWELSNG
                     VGERGRISCAFAVTDVDRELVCDGPVHSKQQIRAALLELLDGLCLELEELGKACRAIK
                     LVLDLQNRTFFEKQFVLHVPTTRKEDMLRRIMAGLEGVELASPVRIMSVRAGALVNHD
                     GRQERLFRSRPDLAKGMKDVSGFLKTKYGALSLARAVRNDSGTHLPDDMFIFVEP"
     gene            1957..2589
                     /gene="lexA"
                     /locus_tag="PKN85_02430"
     CDS             1957..2589
                     /gene="lexA"
                     /locus_tag="PKN85_02430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNT43285.1"
                     /translation="MPRKTDTRENILDFIRTFRRENGYSPSVREVARHCGIKSPSVVQ
                     YHLDQLEQTGLITRDRDRSRSVGIVGEEGEVPQVPLLGVIAAGQPIGVPSADRWNSEA
                     QRMVDVPREVLKSRKDLFALEVRGNSMVDAMIADSDIVVMEQVDDVRNGDVAACWLER
                     EQEVTLKKVYFEKDKVRLQPCNPYMPPLYHDAANVRIQGRVVAVLRMNPR"
     gene            complement(2599..3351)
                     /locus_tag="PKN85_02435"
     CDS             complement(2599..3351)
                     /locus_tag="PKN85_02435"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNT43286.1"
                     /translation="MDGKKRESERHNHRNMSRSGRLIAQAVLFTAALLLLPGPAPMDG
                     AAQPAAEKPYFRKQPLPYPENALEPYISARTLSFHYEKHHQGYVNNLNKLVAGTPLAG
                     RSLEEVVRETAGRPDKTAVFNNAAQVWNHDFFWQCMKKGGGGKPAPGKLLRMIETSFG
                     SFERFANAFRTAAASLFGSGYTWLVQEGDALKIVQTSNADTPIAHGQTPLLTCDVWEH
                     SYYLDYQNRRPDFIGAFLDHLVNWEFVESRVR"
     gene            complement(3579..4475)
                     /locus_tag="PKN85_02440"
     CDS             complement(3579..4475)
                     /locus_tag="PKN85_02440"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNT43287.1"
                     /translation="MKRPVLHTIAVFIFLMLLLPFTSLARDIEPIVSTDWLQQHASDP
                     KLVIVDIRKVEEFKTGHIPNAVNVVYGSFAIMKAGLRNEMPHADDLSDVLSSAGIGAD
                     SHVVVVGNADTVPNRTDISRVAITLRYAGVTNVAILDGGYGKWLAEKKPVATDIVKPK
                     PVKHDIKANEAMFVKKDYVLGAIGKVTMIDTREPEFYEGKKKMDFVAKPGRIKGAVNL
                     PPSQAYTKDGTFKTRAELAALVAKAAGSDPAKEIILYCDTGKVCSSWGYVMTELLGYK
                     NVRIYDGSTEEWMKDPKAPAEP"
     gene            complement(4486..5505)
                     /locus_tag="PKN85_02445"
     CDS             complement(4486..5505)
                     /locus_tag="PKN85_02445"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNT43288.1"
                     /translation="MDRKLLDSDPHFAEGCASCHRGDEKAASREKAHAGLVKRPSTDL
                     GICGKCHDKIAATYANALHFTTAGLRHGVSGRFSASERRVFDEKVFQQSCRSCHASCG
                     DCHVRSPRIAGVSTGLLKGHRFMAKDDGKTCAMCHGGRVYPEFTGDFGGSADIHYQKG
                     MTCLDCHGKSELHGTGTIQTSKREVKERPACEKCHKAGSEKTEKAKTSHAQHKDRVSC
                     YACHSGGVYTNCLDCHLGKGATPKPGFFLGLDPKNRKTVTTLRLVPTVRDTFKTAGIA
                     MEKYDSLPNYWATPTHNIKKRTDRTRSCEVCHVEKRDFLTKEGLIKNGSKANELLLYS
                     PKPLK"
BASE COUNT         1101 a         1598 c         1725 g         1280 t
ORIGIN      
        1 cggcgagatg tgcccgggtg atgagctgcg aaaggttggc gtagcccctg gcgttttcta
       61 tgagaagtgt caggtggtgg tccccttcca gggtgagttc cgctccgatg atgggccgta
      121 ttcccgcctc cctggcctcc ttcgaaaaga tgcaggcgtt gtagagcccg tcgtggtccg
      181 ttatggcaag ggcgcccatg ccgagggacg ccgcccgctc gacaaggctc cgggtcgagg
      241 atgccccgtc gagaaaggaa aagttcgagt ggacatggag ctcggtgtac atggggccct
      301 tcagatagtc cagttttgcc ggtaccacgc gccgcccggc aggtcctgaa agacggtgac
      361 ccgcctgccg ctctccagct cgagggtata atacatgcga acgaccggcc tttgccacca
      421 ggtgtcgtcg acacgccaga tgttgacgat gtctctgacc ttctgtctct tctttctgaa
      481 ggtcacctgc accggccggc cgctgacggc gatcacttcc acgggttcgg gatcgaaggg
      541 ggaggttttc atggctcgac aaagatgaac atgtcatcgg gcagatgggt gccgctgtcg
      601 ttcctgacgg cacgggcgag ggacagggcg ccgtactttg tcttcagaaa accgctgacg
      661 tccttcatgc cttttgcgag gtccggacgg gaccgaaaga gcctttcctg ccggccgtcg
      721 tggtttacga gggcgccggc gcgtacgctc attatacgga cggggcttgc gagttccacg
      781 ccttccagcc cggccatgat cctgcgcagc atgtcttcct tgcgggtcgt gggtacgtgc
      841 agaacgaact gtttctcgaa aaaggtcctg ttctggaggt cgagtacgag ctttatggcg
      901 cggcacgcct tgcccagttc ttcaagctcg aggcaaagcc cgtcgaggag ctcgagaagg
      961 gctgccctga tctgctgttt cgaatggacg ggtccgtcgc agaccagttc acggtcgacg
     1021 tcggttacgg cgaaggcgca ggatatccta ccgcgctccc cgacgccgtt ggagagctcc
     1081 cacaattctt tcccggcagc gccgaactgg gatgtcaggg cggagagggt gaaggcgctt
     1141 atctgcccca gggaatgaag gccaaggaga tgaagcctct cccgcacatc gtcgggtacg
     1201 ggcaggcggt cgataccaag gggggcgagg aactcccttt ccatgcccga aggtatgaca
     1261 cgcgcatcct tcgaggcgca aagcgcggcc tcgaaggcca tgaaacggga attcccggca
     1321 ccgatcctaa cgtcgaggcg gaattgacag gataccatgc tccctaaggc cgatgcgacc
     1381 tgctcctcgc tcttgtacat cccggggagc cgtgtgacat cgagaaagac ggtgccctcc
     1441 tttttcggtt ccatgcggag cgttatcccg gcaatggagg agaggacctc ttcccagaga
     1501 aggctgtgct cccttctgcg cgcgaggacg ggtacggcgt cgtagcagag atggtataca
     1561 tccctgaggg gcatcatggg agcaaccccc ctgtgagcaa gttcctctga gcaatcgatg
     1621 acaaatcccc tctcctcagg catgcccacg atgacgacgg gtctcttccc gaggtcgggg
     1681 tccttcaggc attcgacctg aacatggaaa tgaggcacat gaacacaaac cactcgcata
     1741 acaaacagta tagaacatat gttctagaaa gacaagggaa aagataaaga cggttttaag
     1801 ttttacgttt taggttttaa gtaaaaagaa acagcttcat tatttttcga atggagtgcc
     1861 gcatatcttt tgcctttact taaaacttaa aacctaaaac ttaaaacttc tttttctttt
     1921 cgaaatatag tacacttgtt ctaacgggag ggcacgatgc cgcgaaagac ggatacgagg
     1981 gagaacatac tcgactttat caggaccttc cggcgggaga acggctattc cccgtccgtg
     2041 cgggaggttg cgcgccattg cgggataaag agcccctccg tggtgcagta tcatctcgat
     2101 cagcttgagc agacgggtct catcacgcgc gacagggaca ggtcgaggag cgtcggtatc
     2161 gtcggtgaag agggagaggt cccccaggtg cctcttctcg gtgtcatagc cgccggccag
     2221 cccatagggg tgccctcggc ggacagatgg aactcggagg cgcaacggat ggtagacgtg
     2281 ccccgggagg tcttgaagag caggaaggac ctctttgccc tcgaggtccg gggaaactcc
     2341 atggtggatg ccatgatcgc cgattctgac atcgtggtga tggagcaggt ggacgacgta
     2401 aggaacggcg atgtggcggc atgctggctt gagagggagc aggaggtcac cctgaagaag
     2461 gtctacttcg agaaggacaa ggtgaggctc cagccctgca acccctacat gcctccgctg
     2521 tatcacgacg cggccaatgt ccgcatccag ggaagggtgg tggcggtgct gcgtatgaac
     2581 ccgaggtaaa ccttcccttt atctcaccct cgactccacg aattcccagt tgacgaggtg
     2641 gtcgagaaaa gccccaatga agtcgggacg gcggttctgg taatcgagat aataagaatg
     2701 ttcccagaca tcgcaggtca acaggggagt ctggccatgc gcgatcggcg tatcggcgtt
     2761 ggatgtctgc acgatcttca gtgcgtctcc ctcctgaacg agccacgtgt agccgcttcc
     2821 gaagagggac gcggccgcgg tcctgaaagc gtttgcgaat ctttcgaaac tgccgaaaga
     2881 ggtctcgatc atccggagga gctttccggg ggcgggtttg ccgcctcctc ccttcttcat
     2941 gcactgccag aagaagtcat ggttccagac ctgggcggcg ttgttaaaga ccgcggtctt
     3001 gtccggcctg cccgcggtct ccctgacgac ctcttcgaga gaacgtcccg caagtggtgt
     3061 cccggcgacc agtttgttta gattatttac gtacccttga tggtgttttt catagtggaa
     3121 gctcagcgtc cgggcggata tgtaaggttc cagcgcgttt tcggggtagg gtaggggctg
     3181 tttcctgaaa tagggtttct ccgccgcggg ttgtgcggcg ccatccatcg gggctgggcc
     3241 ggggagcagc agcagtgctg ccgtaaacag aacggcctgc gcgatgagtc ggcccgaccg
     3301 cgacatattc cgatgattat gcctttcgct ttctcttttt ttcccgtcca tggctttcct
     3361 ccttattctt tccatatgaa gagacaccgg gaccggggcc attaaaggga gatgaccgcc
     3421 gccttcgtgc catccagaag tctaccacaa gggtcagggc ggagagagcg cagtcagtcc
     3481 gtcatctgat gtcaggggcc gtggaagggg tcttaaaggg ggtcaaaaaa aggcaccccc
     3541 gcttccatcg ggagtgcctt gaaagaagat ctcaacgact agggttccgc gggtgctttc
     3601 gggtccttca tccattcttc cgttgaaccg tcgtagatcc tgacgttctt gtagccgagc
     3661 aattccgtca tgacgtaccc ccatgaagag cacaccttac ccgtatcgca gtaaaggatg
     3721 atttctttcg cgggatcgga gccggcggcc tttgcgacga gggctgccag ctcggccctg
     3781 gtcttgaatg tgccgtcctt cgtgtatgcc tgtgacggcg gcagattgac agcacccttt
     3841 atcctgcccg gtttcgccac gaaatccatc ttctttttgc cctcatagaa ttcgggctcc
     3901 cgtgtgtcga tcattgtaac cttgccgata gcgcccagaa cgtaatcctt cttcacgaac
     3961 atggcctcgt ttgccttgat gtcgtgcttc acgggtttcg gtttgacgat gtccgtggcg
     4021 actggcttct tctcggccag ccacttgcca tagcctccgt ccaggatcgc cacgtttgtg
     4081 acaccggcgt agcggagcgt tatagccacg cgtgatatgt cggtgcgatt gggcacggtg
     4141 tcggcatttc ccacgacgac gacatgggaa tcagccccga tgccggcgct ggagagaacg
     4201 tccgagagat cgtccgcgtg cggcatctca ttcctcaatc ccgccttcat gatggcgaaa
     4261 gagccgtaga ccacgttgac ggcgttgggg atatggccgg tcttgaactc ttcaaccttc
     4321 ctgatgtcga cgatgacgag tttcggatcg ctcgcgtgct gttgcagcca gtcggtggat
     4381 acgatgggct cgatgtcgcg ggcgagtgac gtgaagggaa gcaacagcat gagaaaaatg
     4441 aagacagcaa tggtgtgcag aaccggtctt ttcatggtct ctcctttatt ttaggggttt
     4501 tgggctgtag agcagaagct cgttcgcctt cgatccgttc ttgatgaggc cctctttcgt
     4561 gaggaagtcc cgtttctcga catggcacac ctcgcaggaa cgggtccggt cggtcctctt
     4621 cttgatgtta tgtgtgggcg tggcccagta attggggagg gagtcgtatt tttccatcgc
     4681 tatcccggcg gtcttgaagg tgtcgcggac cgtggggacg aggcgcaggg tcgtgaccgt
     4741 cttcctgttc ttggggtcga gacccaggaa gaatccgggt ttgggtgtgg cccctttccc
     4801 caggtggcag tcgaggcagt tcgtatacac gccacccgaa tggcaggcat agcagcttac
     4861 cctgtcctta tgctgtgcgt gggaggtttt cgccttttcc gtcttctcgc ttcctgcctt
     4921 gtgacatttc tcgcaggcag gcctttcttt tacctcccgc ttcgatgtct ggatcgttcc
     4981 cgtaccgtgg agttcgctct tgccgtgaca gtcgaggcag gtcattccct tctggtaatg
     5041 gatgtcggcg gatccaccga aatctcccgt gaattcggga tagacccgtc ctccgtggca
     5101 catggcgcag gtcttgccgt cgtctttcgc cataaatctg tggcccttga ggagccccgt
     5161 gctcacaccc gctatccgtg gagacctgac gtggcagtca ccacaggagg cgtggcaact
     5221 tctgcaggat tgctggaaga ccttctcgtc gaataccctg cgctcactgg cggagaacct
     5281 gcccgacacg ccgtgcctga gcccggcagt ggtgaagtga agggcgttgg cgtatgtcgc
     5341 cgcgatctta tcatggcact ttccgcagat cccgagatct gttgacggtc gtttgaccaa
     5401 accggcgtga gccttctccc gggaagccgc cttttcatcg ccgcggtggc aggaagcgca
     5461 gccttcggca aaatgggggt cgctgtcgag aagttttctg tccacgagaa aggctttgtg
     5521 ccgggactcc gccgggaccg acggaggcgc tcccgctcac cccacgcctt cctcgagcac
     5581 caccttcggg tccgcggaat acacggcctc gatatccgag tagatctcgc accgctcggc
     5641 attcagggct gcggcaaggg ccacggccgt ggtgtcactg cccccccgtc caagggtggt
     5701 gatc
//
LOCUS       DAOQTR010000062         5687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_15612_length_5687_cov_4.365767, whole genome shotgun sequence.
ACCESSION   DAOQTR010000062 DAOQTR010000000
VERSION     DAOQTR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15612_length_5687_cov_4.365767"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PKN85_02450"
     CDS             <1..1312
                     /locus_tag="PKN85_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT43289.1"
                     /translation="SFWVVFAAGVLVQLFHLPMLAYSPLLAFLFIVAGIYSHTLERRD
                     QHILDRRLLAITAWLSYTLAATTLFGWGSLSLLAATVWFAVMLNVELGAGLTACLRKI
                     RTVTNYGRTAAGRLVEGVVFPMVFLGLFAVTIFWVILYIGGMPLVTRIIAWHMVVGYL
                     SLNPSMVVVIIAVLFITRSLVVLVNALVTFVATRLGAGTGEGVLKSLHAISTYVIWSL
                     YILLSLKLIGVSVGHLAIIAGGLSIGAGLGLQDMIRNFFSGLVLLFGRSLHPGDEIQL
                     GDVRGTVMKINIRNTVVQTNDDSTIFIPNSDLMYKNIVNWTYRDPRGRAEISVGVAYG
                     SDTEVVRDLLVRCALSHPNVLREPEPYVLFWDFGDSALVFRLRFWIRRPVQMRDKISS
                     AIRFEIDRVFKENTIEIAFPQQDVHIRSADGLNSLPAPEGKPGT"
     gene            1336..2538
                     /locus_tag="PKN85_02455"
     CDS             1336..2538
                     /locus_tag="PKN85_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNT43290.1"
                     /translation="MLKQVGYFDPVPKEDLIGRIDRLRQLMADSGFDLALLLQNVDRF
                     YFTGTMQKGVVAVPLDQDPLVFVEKGTDRAAMETPLNLTPVKNDREIGDILRGRRLLA
                     GKVGLELDVLPVAVFERMKRVFDFERCGDVSEQVREVRAVKSRFELDQIVMSGRMLTS
                     VFSLARDEVREGRTELGIEAALTAAGREMGHQGFLRMRGMNQEMMTMTVQAGFTGAIP
                     TLLDAPITGTGVTPAVPQGSSFKKVERGVPVTIDYGGGYNGYITDETRSFVVGDLAEP
                     FRKPYETARSIIEDVLSFAGPGTDCRDIFHRAEGMARKAGLSDLFMGHGEGKVAFIGH
                     GLGLEINELPVLTPRHERILKEGMVFAFEPKFVLPDLGAVGMEIDLIVGSERLERVTT
                     GPFDLVCL"
     gene            2590..3234
                     /locus_tag="PKN85_02460"
     CDS             2590..3234
                     /locus_tag="PKN85_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNT43291.1"
                     /translation="MSGTWGVIVIDMQGDFTEWKRGSLAVPGSDEVYARNVESVTRQL
                     KDLGIPILGTQDWHPPDHVSFATSHPGRKPFDTVVIDGETQVLWPPHCVQGTENARIV
                     IDNNLFLAVVKKAQDPSTESYSAFQAGGELERILQWNGIDRVIVYGLATDYCVRATSL
                     DLTAAGYLVVVIEGLCRSVAPDTAAAAVDEMRSKGVRVVATPGEAIEEVRRNSG"
     gene            3694..4143
                     /locus_tag="PKN85_02465"
     CDS             3694..4143
                     /locus_tag="PKN85_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT43292.1"
                     /translation="MPEQIRRILGKHGRSPDELIPILQDIQAAFGYIGEPQVKAISRY
                     LRISENQIYGVSSFYAQFRFTEPGRHGIKVCLGTACHVRGGGTLLDSLEHGLGIHCGE
                     TTEDKRFDVDRVACLGCCALSPVVQIDRDIYSRMTVNKLTELLKNYE"
     gene            4136..>5687
                     /locus_tag="PKN85_02470"
     CDS             4136..>5687
                     /locus_tag="PKN85_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT43293.1"
                     /translation="MNKLVKKIDTAKGKWDKLKDSREPIVYVGAASCGRAAGALELKE
                     AVKAFLDENLKSARVIEVGCIGPCYLEPLVDIKMPGQPRVSYNNVSAKTLQMILKSHF
                     VNGEPHKKLALGHFGTDDFDGIPKFYDLPMLKPQVRIVLRNCGLIDPEEIDQYLAVDG
                     YQGFMKALKSTPEDVIATVRQAGLRGRGGAGFPTFRKWTVCRESPGEQKYLICNADEG
                     DPGAFMNRSLIESDPHAVLEGMLIAGYAIGASKGIVYIRAEYPLAIERLKRAIGQMRE
                     YGLLGKNILGSDFSFEIKIKEGAGAFVCGEETALIGSIEGKRGMPRSRPPFPAIAGLF
                     GAPTIINNVETLGTLPNILRNGPEWYTRFGKEGNRGTKTFSLVGKIRRPGLIEVQLGT
                     TLREIIFDIGGGVQKPFKAIQTGGPSGGCLSEEFLDLTVDYESLASAGSIMGSGGLIV
                     MDEDTCIVDLAKYFLDFTQKESCGKCVPCRVGTKHILEILQKITNGEGTPDDLLALRT
                     LGDTIKKGA"
BASE COUNT         1145 a         1637 c         1741 g         1164 t
ORIGIN      
        1 gtctttctgg gtggtattcg cagcgggtgt gctcgtgcag ctcttccatc tcccgatgct
       61 tgcctactct cccctgctcg ctttcctctt catagtggcg ggcatctact cacacacact
      121 tgagagaagg gatcagcata tcctggacag gagactgctt gccataacag cctggctgtc
      181 gtacacactc gccgccacga ccctcttcgg ctggggcagc ctctccctcc tcgcggccac
      241 ggtgtggttc gcggtcatgc tgaacgtgga actgggggcc ggattgacgg cgtgcctgcg
      301 caagatcagg acagtgacaa actacgggcg aacggccgcc gggaggcttg ttgaaggcgt
      361 tgtcttcccg atggtcttcc tcggcctctt cgctgtcacc attttctggg tgattctcta
      421 tataggcggc atgcctcttg tcactaggat catcgcgtgg cacatggttg tcggttatct
      481 ctcgctgaat ccttccatgg ttgtcgttat catagccgtt cttttcatca cgcggtccct
      541 tgtcgttctc gtcaatgccc tcgtcacgtt cgtggctacc cgccttgggg cggggacagg
      601 agaaggcgtt ctcaagtcgc ttcatgccat atccacctac gtgatatggt cattgtacat
      661 tctcctctcg ttgaagctga taggggtaag cgtcggccac ctggctatca tcgccggcgg
      721 attgtccata ggagccggcc tggggctcca ggacatgatc aggaacttct tcagcggcct
      781 cgtgctcctt ttcggcaggt cgctccatcc cggcgacgag atccagcttg gcgatgtacg
      841 ggggacggtg atgaagataa acatccgcaa caccgtcgtg cagaccaacg acgactccac
      901 gatcttcatc cccaactcgg acctcatgta caagaacatc gtcaactgga cataccgcga
      961 cccccggggc agggccgaga tatccgtggg agtggcatac ggctccgaca cggaggtcgt
     1021 cagggacctg ctcgtccggt gtgccctttc ccatccgaat gttctcaggg agcccgaacc
     1081 ctacgtcctc ttctgggatt tcggagacag cgccctcgtg ttccgcctga ggttctggat
     1141 acggcgtccc gtgcagatgc gtgacaagat atcatcggcg atacgttttg agatagacag
     1201 ggtgttcaag gagaacacca tcgagatcgc tttcccccag caggatgtcc acatccgttc
     1261 cgccgatggt ttgaattccc tcccggcccc ggaagggaag ccggggactt gaatctcccc
     1321 ctgacgaggt ggaatatgtt gaaacaggta ggttacttcg atcccgtgcc gaaagaggac
     1381 ctcatcggca ggatcgacag gttgcggcag ttgatggcgg attcaggatt cgatctcgcg
     1441 cttctcctgc agaacgtcga caggttctat tttacgggga ccatgcagaa gggcgtcgtt
     1501 gccgtgcccc ttgaccagga ccccctcgtt ttcgtggaaa agggaacgga ccgtgcggcc
     1561 atggagacac cccttaacct cacgcccgtc aagaacgacc gggagatcgg cgacatcctc
     1621 cgcggccgtc gcctcctcgc gggaaaggtg ggcctcgagc ttgacgtgct gcccgtcgcc
     1681 gtctttgaga ggatgaagag ggtcttcgac ttcgagcgtt gcggcgacgt gtcggagcag
     1741 gtcagggaag tgcgggccgt caagagccgc tttgagctcg accagatcgt gatgtccggc
     1801 cggatgctga ccagcgtctt ctcgctggca cgggacgagg tccgcgaggg caggacggag
     1861 ctcggtatcg aggcggccct cacggcggcc gggagggaaa tgggccacca ggggttcctc
     1921 agaatgagag ggatgaatca ggagatgatg acgatgaccg tgcaggcggg attcaccggt
     1981 gccattccca ccctgctcga cgccccgata acggggaccg gggtcacccc cgccgtcccc
     2041 cagggttcct ccttcaagaa ggtcgaaagg ggagtccccg tcaccataga ctacggcgga
     2101 gggtacaacg ggtatatcac cgacgagacc aggagctttg tggtgggcga cctggcggag
     2161 cctttcagaa agccctatga gacggcacgg tcgatcatag aggatgtgct gtcttttgcg
     2221 gggccgggga ccgattgccg ggacatcttt caccgcgccg aaggcatggc acgcaaagct
     2281 ggcctcagcg acctcttcat ggggcacggt gaaggcaagg tggctttcat cggccacggc
     2341 ctcggtctcg agatcaacga attgcctgtt ctaacgccgc gccacgaacg gatactgaag
     2401 gaagggatgg tgtttgcctt cgagccgaag ttcgtgcttc ccgacctcgg agcggtgggt
     2461 atggagatcg atctcatcgt tggttcggag cgtctcgaga gggtcacgac aggtcccttc
     2521 gacctcgtgt gcctttaatc gccttcacgt cctttgacgg atctcgaccc gtgtggtaag
     2581 agtagagata tgtctggcac atggggtgtc atagtcatcg acatgcaagg cgacttcacg
     2641 gagtggaaac ggggcagcct tgccgttccc ggatccgatg aggtgtacgc gagaaatgtc
     2701 gagagtgtca cgcggcagct caaagatctc ggcatcccca ttctcggcac ccaggactgg
     2761 catcctcccg atcacgtttc cttcgcgacg agccacccgg ggaggaagcc cttcgacacg
     2821 gtggtcatcg acggggaaac gcaggtcctc tggcctcccc attgcgttca gggaacagag
     2881 aacgcgcgca ttgtcataga caacaatctc tttcttgccg tggtgaagaa ggctcaggat
     2941 ccctcgaccg aaagctattc ggcatttcaa gccgggggcg agttggagcg tattctgcag
     3001 tggaacggga tagacagggt gatagtttac ggactcgcga ccgactactg cgtcagggcc
     3061 acgtcccttg acctcaccgc tgccggttac ctggtggtcg tcatagaagg cctttgccgg
     3121 tccgtggctc ccgacacggc cgcggccgct gtcgacgaga tgaggagcaa aggggtgcgt
     3181 gttgtggcca caccgggaga ggcgatcgaa gaggtccgca ggaacagcgg ttgatcgatc
     3241 cctggattcc acccggctgc ggcccgcgat caccggtcca tcaatccata cttcatccca
     3301 tccggtattg ccgcacggcc cttcccgtca caggaccagg ccggaggggc gagcgggtcg
     3361 gtcctccgta tcaacaaaac ggtatactgt atccagaccg catgctcacg tcttctttcc
     3421 cggaccccgg gcgggagcgc cccgggggcc gtttggcgcc tcgcggctaa cttgcggtaa
     3481 ttgcttgtgg tccatttttt ttatggaatt tttcttgaca cgaatttgaa cgttctttaa
     3541 taatgagagt acatgggggc aaattcggga agtgatcgtc tgctgattca acaatcacgt
     3601 gacctttgtc tagatctcat cttacctgtt cgaacgaccc gcggcgcgac tgatctcagt
     3661 taacgccaca cgaatgtggc ataggagaga tccatgcccg aacaaattcg aaggatcctc
     3721 gggaagcatg gccgcagtcc cgatgagctg atcccaatac tgcaggatat ccaggcggcc
     3781 ttcgggtata tcggagagcc tcaggtaaag gctatctccc gataccttcg catatcggaa
     3841 aatcagatct acggtgtctc atcattctac gcgcaattcc ggttcaccga accgggacgc
     3901 cacgggataa aggtctgtct cggtacggcc tgtcatgtcc gcggtggcgg gacgctgctc
     3961 gacagcctgg agcacgggct gggcattcac tgcggagaga cgaccgagga caaacggttc
     4021 gacgtggacc gtgttgcctg cctcggatgc tgcgcgcttt cgccggtggt gcagatcgat
     4081 cgtgatattt acagcaggat gacggtcaac aaactgacgg agttattgaa aaattatgaa
     4141 taagctcgtt aagaagatcg atacggcaaa gggtaaatgg gacaagctga aggacagcag
     4201 ggagcccatc gtttacgtcg gcgccgcgtc ctgcggcagg gccgccgggg ccctcgaact
     4261 caaggaggcg gtgaaggcct tccttgacga gaacctgaag tccgcgcgcg tcatcgaggt
     4321 gggctgcatc gggccctgtt accttgagcc cctcgtggac atcaagatgc ccgggcagcc
     4381 ccgcgtgagc tacaacaatg tgagcgcgaa gaccctgcag atgatcctta agtcccactt
     4441 cgtgaatgga gaaccccaca agaagcttgc cctcggacat ttcgggaccg acgatttcga
     4501 cggcattccg aagttttatg accttcccat gctcaaaccc caggtgcgga tagttttgcg
     4561 caactgcggt ctcatagatc ccgaggagat cgaccagtat ctcgcggtgg acggttacca
     4621 ggggttcatg aaggcgctca agtcaacgcc cgaggatgtc atcgcgacgg tccggcaggc
     4681 cggcttgaga gggcgcggcg gcgccggttt cccgacgttc aggaaatgga cggtctgcag
     4741 ggagtctccg ggcgagcaga agtacctcat ctgcaacgcc gatgaaggcg accccggggc
     4801 tttcatgaac cggtctctta tcgagtccga cccacacgcg gtgctggagg gcatgctcat
     4861 cgcaggctac gccatcggcg cgagcaaggg catcgtctat atccgcgccg aatatcctct
     4921 ggccatcgag aggctcaaga gggccatcgg gcagatgcgg gagtacgggc ttctggggaa
     4981 gaatattctc ggctcggatt tcagtttcga gataaagata aaggaagggg cgggggcctt
     5041 cgtctgcggt gaggagacgg cgcttatcgg gtccatcgag ggaaaacggg gcatgccccg
     5101 ctcgcgcccg cccttcccgg caatagcggg cctcttcgga gcgcccacca tcatcaacaa
     5161 cgtggagacc ctggggaccc tgccgaacat cctgaggaac gggcccgaat ggtatacccg
     5221 gttcggcaag gagggcaaca gggggacgaa gaccttctcc ctcgtgggca agatccgccg
     5281 gcccggcctc atcgaggtcc agctcggaac caccctgcgg gagatcatct tcgatatcgg
     5341 gggcggcgtg cagaagccct tcaaggccat acaaacggga gggccctcgg gcgggtgcct
     5401 gtcggaggag ttcctcgatc ttaccgtcga ctatgaatcg ctggccagcg ccggttccat
     5461 aatgggttcc ggagggctca tcgtcatgga tgaggacaca tgcatcgtcg acctcgcgaa
     5521 atacttcctc gatttcaccc agaaggagtc ctgcgggaag tgcgtgccct gcagggtggg
     5581 tacgaagcat atcctggaga tccttcaaaa gataacaaac ggcgagggga cacccgacga
     5641 cctcctcgcg ttgaggacgc ttggcgacac gatcaagaag ggggccc
//
LOCUS       DAOQTR010000063         5637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_15774_length_5637_cov_3.760301, whole genome shotgun sequence.
ACCESSION   DAOQTR010000063 DAOQTR010000000
VERSION     DAOQTR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15774_length_5637_cov_3.760301"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKN85_02475"
     CDS             complement(<1..352)
                     /locus_tag="PKN85_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNT43294.1"
                     /translation="MTVNRLETDKMREIKAACACLFSEAAAKSDRFLDTLDVDMVKRA
                     YRRNAKVHHPDMKASTAVNDVTSDRFLAIQQSYEVLVNYLEGMNPGTDAVLSQGKIVA
                     VGGAKGGIGKSVITA"
     gene            complement(643..1287)
                     /locus_tag="PKN85_02480"
     CDS             complement(643..1287)
                     /locus_tag="PKN85_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43295.1"
                     /translation="MSVLFTQSWDIIQGKDVEYAQFIVDTYLPEMAEMGLVPVGGYYV
                     EVGFGPRIVAVFSVNETEEVLKTIAGKRFKDLLRRLKTIIMNYRGSILEPTGAVKRGK
                     YTIQKGVWKLNQYYDLKPGVKKAYADFVINEHIPTVQKINYVEITGGWNVLIGGISEI
                     IAEFTFKDAMDVGRLLMNEDYRRITQKLRTEYAMNYTSRVMKSTERFDEPRWFK"
     gene            complement(1298..3649)
                     /locus_tag="PKN85_02485"
     CDS             complement(1298..3649)
                     /locus_tag="PKN85_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT43296.1"
                     /translation="MKAYNVINTRLPRVDGRAKATGDARYAADLSMPGMLYGAMLQSP
                     LAHAKILNIDTSAAKRLPGVRDVVTWKEAGLVKYGVSPARYDEQVFCSDKVRYVGDEV
                     AAVAAIDLETAMEAVSLIKVDYEELPVLLDIGSAMAEGAIAIHEDFPGNISAEVHQEF
                     GNFEEALKECHIVRTDTFTNKRQDGGFLEPQACLARYDLNGNLTLWSSTQSVHYIQRS
                     VAMVLGIPVGKVRVVKPYVGGGFGPKAAVNAMELAACLMSRRTGKPVKMVFSREQVFL
                     HSRARHQFTHELTTGVKKDGTIFALRNVCHLDGGAYSSFGIATIYYAGSLLGAPYKLP
                     NMKYDGYRIYTNKPACGAQRGHGGVAARAGWEQQLDIIAAELGMDPVEFRLKNMMQTG
                     DVTCNDFNMSSLGMKECLEGVRDGSNWGAKKGKLPKGKGIGVACGFFVSGAGYPIYRS
                     ETFHSTATIRLTEDGGGVNLYTASAEIGQGSDTILGQIAAEALGVSLDDVRVHSGDTD
                     FGVDLGAYSSRQTLMSGHAVKMAAEDTKRKVLEALSEQLNVPVEEMDIARGVIVFKKD
                     KPDFSRLRTLYIKEHRGWADQPSGAELTFREAARVAFITGGVIIGNGAYKPGELGGKY
                     KGAAVGTSPAYGCSAQVVEVTVDEETGKVTIDAMTDAHDCGFAINRTNVEGQMQGSLS
                     MGLGEALFEEVRFDEKGRVANASLGEYRIPTALDMPNVKTIIIESDEPNGPFGAKEVG
                     EGAIMPTIPAILNAIYDAVGVRINELPITSERLFAAMKEKRKK"
     gene            complement(3669..4139)
                     /locus_tag="PKN85_02490"
     CDS             complement(3669..4139)
                     /locus_tag="PKN85_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT43297.1"
                     /translation="MKKLITFTVNDMGYEVAVAPNKTLTQVLRDDLDLLGTKEGCGVG
                     DCGACTVIMDGKPVNSCLVLAVQADGSTIKTIEGVARGDSLHPVQETFVEMGAIQCGF
                     CTPGMVLSATSLLEKNPHPTEHEVREALSGNLCRCTGYQKIVEAVQEASRRMKG"
     gene            complement(4139..5035)
                     /locus_tag="PKN85_02495"
     CDS             complement(4139..5035)
                     /locus_tag="PKN85_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNT43298.1"
                     /translation="MLLLPKFDYEEPKSLPEALAMLSELKGNGKVIAGGTDLLVNMKK
                     GTVKPAFLVSLRKVEGLREIGGENGGLAIGSHATISEIADSDRVGSMFPVLARSASCL
                     GSPLIRNRATIGGNIVTARPAADLPPALIVLGAYVELKGKDANRTIPLDGFFTGPGTS
                     VIESGEVLTRIILPSAPPFTGGDYIKIGHRAALEIAIVAAASRLTLDKPDGVIVDARV
                     VLSAVAPTAIHAASAEKALIGQRPSEELFAKAAALAEGDCSPITDMRGGAEYRRDMVN
                     TLVKRTLLKAFNDARDMKGGNA"
     gene            complement(5173..5373)
                     /locus_tag="PKN85_02500"
     CDS             complement(5173..5373)
                     /locus_tag="PKN85_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNT43299.1"
                     /translation="MDIIFNGVRENVPEKTAISDLIVKTDEKDGGLIVEVNRRFVFPA
                     DYGKTFLSEGDTVEFINPDFGG"
     gene            complement(5385..5567)
                     /locus_tag="PKN85_02505"
     CDS             complement(5385..5567)
                     /locus_tag="PKN85_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43300.1"
                     /translation="MMDDAKIAEMDRKVEALREMVQDLIDSAGDVEAVKRNAKRILAS
                     VKMLEINICDVAATRV"
BASE COUNT         1067 a         1787 c         1521 g         1262 t
ORIGIN      
        1 tcgccgtgat gacactcttc cctattccgc ccttcgcgcc tcccacggca acgatcttgc
       61 cctgggaaag gacggcatcg gtccccgggt tcatcccttc caggtagttg accagcacct
      121 cgtaggactg ctgtatcgcg agaaagcggt ccgatgtcac atcgttgacg gcggtgctcg
      181 ccttcatgtc aggatggtgt accttggcgt tgcgccggta ggcccttttc accatgtcaa
      241 catcgagcgt atccagaaac cgatcactct tcgcagccgc ctcagagaac aaacaggcgc
      301 aagccgcctt tatttcccgc atcttatccg tctccagcct gttcaccgtc atctatcgct
      361 atcctcgatc aggtattcga caacacgagg attgtagtgc aataagtgta ccagaaaaag
      421 accgttgcaa ccgcttgagc cgctttaacg ttaaaagaaa caaagccttg tcgccgttat
      481 ttgggtgata gatccgggat gcctgctcac gttgagagca tttcaggaaa gaatggcagg
      541 cagagaaatc actaccgagg aggctccagc ctggggcaac cgcaagaccc ccgtccttga
      601 ccgttcaagc gattcaagcg gctcgagcgg ttgcaacggt ctttacttga accaccgcgg
      661 ttcgtcgaag cgttccgtgc ttttcattac cctgctcgtg tagttcatgg cgtattcggt
      721 gcggagtttc tgggttatac gccggtagtc ttcgttcatc aggagcctgc cgacgtccat
      781 ggcgtccttg aaggtgaatt cggcgatgat ctcactgatg ccgccgatca ggacgttcca
      841 gccccctgtt atctccacgt agttgatctt ctgcaccgtg gggatgtgtt cgtttatgac
      901 aaaatcggcg taggcctttt tgacgcccgg cttcaggtcg tagtactggt tgagcttcca
      961 gacgcccttc tggatggtgt acttgccgcg tttgaccgcg cctgtgggtt ccaggatgga
     1021 accccggtag ttcatgatga tggtcttcaa gcgacggagg aggtccttga acctctttcc
     1081 cgcgatggtc ttcaggacct cctccgtttc gttcacactg aacacggcaa ctatgcgggg
     1141 accgaagcct acctcgacat agtagccgcc gacgggcacg agacccatct cagccatttc
     1201 cggcagatag gtgtcgacta tgaactgtgc atattcaaca tccttcccct ggattatatc
     1261 ccagctctgc gtgaacagca cagacatgcc gtcctcctta tttctttcgc ttctccttca
     1321 tcgccgcaaa aagacgctct gaggtgatgg gaagctcgtt gatcctcacg cccaccgcgt
     1381 cataaatggc gttgaggatg gcgggaatgg tgggcatgat cgcgccctcc cccacctctt
     1441 tggccccgaa aggtccgttc ggttcgtcgc tctcgattat tatggtcttg acgttcggca
     1501 tgtcaagcgc cgtagggatg cggtattctc ccagagaggc attcgcgacc cggcccttct
     1561 cgtcgaatct tacctcctca aagagcgcct cgccgagacc catcgagaga gatccctgca
     1621 tctgaccttc cacgttcgtg cgattgatgg caaagccaca gtcgtgggca tcggtcatgg
     1681 catcgatggt gaccttgccc gtctcctcgt ccaccgtcac ttcgacgacc tgggcggagc
     1741 atccgtatgc cggcgatgtt cccaccgcgg cacccttgta cttacctccc agctcgccgg
     1801 gtttgtaagc gccattaccg attataacac ccccggtgat aaaagcaaca cgcgccgcct
     1861 ccctgaaggt gagctccgca cccgaaggct ggtctgccca tccgcggtgt tccttgatat
     1921 aaagcgtccg caaccgggaa aaatccggtt tatccttctt gaaaacgatg acgcccctgg
     1981 caatgtccat ctcctcgacc ggcacgttaa gctgctccga aagcgcctcg agaaccttcc
     2041 gcttggtgtc ttccgcggcc atcttgacgg catggccgga catgagggtc tgccgtgacg
     2101 aataggcacc gaggtcgacg ccgaaatcgg tgtcccccga gtgcacccgc acgtcatcga
     2161 gagagacacc gagggcctcc gcggcgatct gcccgaggat ggtgtccgac ccctgcccta
     2221 tctccgcaga cgcggtataa agattgaccc cgccgccgtc ctcggtgagc ctgatcgttg
     2281 ccgtggaatg gaacgtctcg gaccggtaga tgggataccc cgccccggac acgaagaacc
     2341 cgcaggccac acctatcccc ttacctttgg ggagtttccc cttcttggcg ccccagttcg
     2401 agccgtcgcg taccccctcg aggcactcct tcatgccgag gctcgacatg ttgaagtcgt
     2461 tgcaggtaac gtcgccggtc tgcatcatgt tcttgagcct gaactccacg gggtccatgc
     2521 cgagctcggc agcgatgatg tcgagctgct gttcccatcc cgccctcgcg gcaacgcctc
     2581 cgtgcccgcg ctgagcaccg caggcgggtt tgttcgtata gatgcgataa ccgtcgtatt
     2641 tcatgttcgg aagcttgtag ggagcgccga ggagcgaacc ggcatagtag atcgtcgcga
     2701 tgccgaagct cgaatacgcg ccgccgtcaa gatgacagac gttcctcaag gcgaagatgg
     2761 tcccgtcctt ctttacgccc gtcgtcaatt cgtgggtgaa ctggtgccgt gcccgggaat
     2821 ggaggaagac ctgttcccgg gagaagacca tcttcaccgg tttgcccgtg cgtctcgaca
     2881 tgaggcacgc cgcgagctcc atggcgttca ccgccgcttt ggggccaaaa ccgcccccca
     2941 cgtagggctt cacgacacgg accttgccga cggggatgcc gagcaccatg gcaacgctcc
     3001 tctggatata gtggaccgac tgggtcgacg accacagcgt gaggttcccg ttcaggtcgt
     3061 accgggcaag acaggcctgg ggttccagaa agccgccgtc ctgccttttg ttcgtgaacg
     3121 tgtcggtcct gacgatgtgg cactccttca gggcctcctc gaagttgccg aactcctggt
     3181 ggacctcggc actgatgttt ccgggaaaat cctcatgtat ggcgatcgcc ccttccgcca
     3241 tggccgatcc gatatcgaga agcacaggaa gctcctcgta atcgaccttg ataagggaca
     3301 ccgcctccat cgccgtttcg aggtcgatgg ccgcgaccgc agccacctcg tcgccgacat
     3361 aacgaacctt gtcggagcag aagacctgct cgtcgtaccg cgccggactg acaccgtact
     3421 tgacgagccc cgcctccttc catgtgacca cgtccctgac cccggggagt ctcttcgccg
     3481 cggacgtgtc gatattcagt atcttcgcgt gcgccagagg gctctgcagc atcgccccgt
     3541 agagcatgcc gggcatggag aggtccgccg cgtagcgggc gtcacccgtc gccttcgccc
     3601 tgccatccac cctgggcagc cgcgtattta tcacgttgta tgccttcata gtccttgcct
     3661 cctcctcgtc atcccttcat tcttctggac gcttcctgca cggcctccac gatcttctga
     3721 taccccgtgc accggcagag gttgcccgaa agggcctctc ttacctcgtg ctctgtcggg
     3781 tggggattct tttcgagaag gctcgtggcg gaaaggacca ttccgggggt gcagaaaccg
     3841 cactggatgg cacccatttc cacgaatgtc tcctggacag gatgaaggga gtcgccgcgt
     3901 gccacccctt ctatcgtctt tatggtggag ccgtcagcct ggacggccag gaccaggcag
     3961 gaattcaccg gcttgccgtc catgatcacg gtgcaggcgc cgcaatcgcc cacgccgcat
     4021 ccttcctttg ttcccaggag gtcgaggtcg tcacgcaaca cctgggtcag ggtcttgttg
     4081 ggggcaacgg ccacctcgta tcccatgtcg ttcactgtga aagtgatcag ttttttcatc
     4141 acgcattccc ccctttcatg tcccgggcat cgttgaaggc cttgagcaag gtcctcttta
     4201 caagggtgtt caccatatcc cggcggtatt cggccccgcc gcgcatgtcg gtgatgggag
     4261 aacaatcacc ttccgcgagg gcagcggcct tcgcgaagag ctcttccgac gggcgctggc
     4321 cgatcaaggc cttttccgcc gacgcggcat gtatcgcggt gggagcaacg gcactgagga
     4381 caacgcgcgc atcgacgatc accccgtcgg gtttgtcgag cgtcaaccgt gaggccgccg
     4441 cgacgatggc gatctcgagc gccgccctgt ggccgatctt gatataatct ccgcccgtga
     4501 atggcggcgc ggaggggagg atgatccgtg tcagcacctc gccgctttcg atcaccgagg
     4561 ttcccggtcc cgtgaagaaa ccgtccagcg gaatcgttct gttcgcatct ttccccttca
     4621 gttccacgta agcccccagg acgatgagag cgggcggcag gtccgccgcc ggccgggcgg
     4681 tcacgatatt tccgccgatg gtggcgcggt tcctgatgag cggggagccg agacaggacg
     4741 cggaccgggc gaggaccggg aacatcgaac cgacgcggtc cgagtcggcg atctccgata
     4801 tcgtcgcgtg ggacccgata gcaagcccgc cgttctcccc gccgatctcc cgaagcccct
     4861 ccaccttcct caggctcacg aggaatgccg gcttgacggt ccccttcttc atgttcacga
     4921 gaagatccgt gccccccgcg atcacctttc catttccctt caactccgaa agcatcgcca
     4981 gggcttccgg caggctcttc ggctcctcat agtcgaactt aggcagcaat aacatgaaac
     5041 ctcctctcat agatgcggtt caagggttcc agagttcaag ggttcccgag ctaaaaccaa
     5101 acaaagccga aaattgcgga acttttgaac tcttgaactc ttgaactttt gaactcttga
     5161 accgtttctc tcctatcctc caaaatccgg gttgatgaat tctaccgtat cgccctccga
     5221 caggaaagtc tttccgtagt cggcgggaaa gacgaacctc ctgtttactt ccacgatcag
     5281 tcctccgtcc ttctcatctg tctttacgat caggtccgat atggcagtct tctccggtac
     5341 gttctcgcgc acgccgttga atattatgtc catgttcctt ctgctcagac tcgggtggcg
     5401 gcgacgtcgc agatgttgat ctcgagcatc ttcacgctcg ccagaatgcg cttcgcgttc
     5461 ctcttcaccg cctccacgtc tccggccgaa tcgataaggt cctgcaccat ctccctgaga
     5521 gcttccacct tcctgtccat ctcagctatc ttcgcatcat ccatcatagt catctcctgg
     5581 atataataat ggttcaaaag ttcaaggttc aaggttcaag gcaaaaaacc gggggaa
//
LOCUS       DAOQTR010000064         5610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_15877_length_5610_cov_3.410981, whole genome shotgun sequence.
ACCESSION   DAOQTR010000064 DAOQTR010000000
VERSION     DAOQTR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15877_length_5610_cov_3.410981"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKN85_02510"
     CDS             <1..993
                     /locus_tag="PKN85_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNT43301.1"
                     /translation="EGGFKRNESNPLDAGLIVIDEASMVDVSLMYHLLKAVPPPATLV
                     LVGDVDQLPSVGPGSVLKDIISSGCVRTVRLDEIFRQSTTSMIIVNAHRINAGEMPFF
                     EKHTGQGHDFFFVDIEDPDEVLRYVVRLCRDVIPTRFGLHPVSDVQVLTPMHRGVVGV
                     SNMNARLQEALNASTDGISRGGRFFKRGDKVLQTRNNYEKDVYNGDIGTVIAMDREVQ
                     ELRVDFEGRVVSYDFNEVDELILAYAISVHKSQGSEYPAVVMPILTQHYLLLQRNLLY
                     TAITRGKKLVYLVGTKKALSIAIRNDKPHRRYTLLAERLRETKAVPGPRSHVPG"
     gene            1273..1485
                     /locus_tag="PKN85_02515"
     CDS             1273..1485
                     /locus_tag="PKN85_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43302.1"
                     /translation="MTIGRGTKYAGRARCFCAWCGRERKRNGVWERSELNAEISRVAV
                     THGICPDCARGLLGGRTKDKAMKRAD"
     gene            1698..3518
                     /gene="glmS"
                     /locus_tag="PKN85_02520"
     CDS             1698..3518
                     /gene="glmS"
                     /locus_tag="PKN85_02520"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNT43303.1"
                     /translation="MCGIVGYEGTREACDILIDALSRLEYRGYDSAGIALLGQGEIRI
                     ERRKGKVEELRKVLKGAALKGSIGLAHTRWATHGVPSERNAHPHRAGDVVVVHNGIIE
                     NYLELKEHLKGAGHVFISDTDTEVIPHLITDHARKGRDLVDSVRLALKELKGSWAVGI
                     LKESERTLVAAKNQSPLIVGVGDGEYFIASDIPAIINMTNRFIFLEDNDMAIFKGRDM
                     RIINVDGEDVVREVRQVCWTAGMAEKGGYSHFMLKEIFEQPQAISDTLLGRAREEEGE
                     VEFEGVDLPDPAGIRKIWMVACGTSHHACMIGKNLIETSLRIPVETDMASEFRYRDPI
                     VSPRDLFIAVSQSGETADTIAAMREARSRGASTLSICNVLGSTLAREAENVVFTHAGP
                     EIGVASTKAFTTQISVMFLLTLCIGKRLGIFGPQDVRERLREIRAIPRKVRSVLDEAP
                     SIQAAAARYMFFRSFLFLGRGINFPTALEGALKLKEISYIHAEAHAAGEMKHGPIALI
                     DDDMPVVCISPADRTYKKACGNMEEVMARKGNIILISDDETHEMAPRAGEMITVPRTA
                     YELQPILSIVPLQLLAYYIADLLGMDVDQPRNLAKSVTVE"
     gene            3573..4982
                     /locus_tag="PKN85_02525"
     CDS             3573..4982
                     /locus_tag="PKN85_02525"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT43304.1"
                     /translation="MIRVLIIDGGRQTCDMLAGCALSLGYDVSTGQTLTECLEKVTSN
                     PPDVIFIDGQLPDGSPLEVLDVIHAAPSSPTVIIMTEGGTPESAEMAIRNGAWDYIPK
                     PASMKEMTARLSRVAQYRRQHQGSERMAEIKRNGIVGNSPTMRTAMDMIKRAADSDLN
                     VLITGETGTGKELFALSIHLNSGRSQDNFVVVDCTALPETLVESILFGHSKGAFTGAD
                     RAQDGLIRQAHRGTLFLDEMGELPLPLQKTFLRVLQEHRFRPVGSQKEVESDFRLISA
                     TNKNLEQMVAEGKFRGDLLYRLGAMTIHLPPLRQHPEDIREMAVFYVEKVCRRFGVAP
                     KKFSDEFLKYLIRYDWPGNVRELFHVLEHACAAGLHEETLFPVHLPQELRITIARSLF
                     EDNHHVPRNGGNGSGSSAAKQILSLKRFRQSAYSDLEEMYLRNLLTSSAGSIKEACAL
                     SKLSRTRLYGLLKKYNIRH"
     gene            complement(5152..>5610)
                     /locus_tag="PKN85_02530"
     CDS             complement(5152..>5610)
                     /locus_tag="PKN85_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43305.1"
                     /translation="RVLDAGQCNDSYSLVVIAQALAKAFGVGINELPVSYNIAWYEQK
                     AVLVLLALLSLGVKDITLGPKLPAFISPAVLDVLVKEKGFQHAPELPGKVLPILGVED
                     GVVPFLCPRSHSWAPLLRTALSRRSFCVFSIHNDYRISITVPPALTFIKR"
BASE COUNT         1273 a         1570 c         1745 g         1022 t
ORIGIN      
        1 gaggggggat tcaagcgcaa cgaatcgaac cctctcgacg cggggcttat cgtcatcgac
       61 gaggcttcca tggtcgatgt ctccctcatg taccaccttc tgaaggcggt acccccgccc
      121 gcgaccctcg ttctcgtcgg tgacgttgac cagcttccct ccgtgggtcc cggaagcgtc
      181 ctgaaggaca tcatctcctc ggggtgcgtg aggacggtaa ggctcgatga gatattccgg
      241 cagtcgacga caagcatgat catcgtcaac gcccatcgca taaacgccgg cgagatgccc
      301 ttttttgaga agcacaccgg gcagggccac gatttcttct ttgtcgatat cgaggatccc
      361 gacgaggtcc tgcggtatgt tgtccgtctc tgccgggatg tgatacccac ccgtttcggc
      421 cttcatccgg tgagcgacgt ccaggtcctg acgccgatgc accgcggagt cgtgggtgta
      481 tcgaacatga acgcacggct ccaggaagcc ttgaacgcct ccacggacgg catctctcgt
      541 ggaggcaggt tcttcaagag gggcgacaag gtccttcaga cgcggaacaa ttacgaaaag
      601 gacgtgtaca atggagacat cgggacggtg atcgccatgg acagggaggt gcaggaactc
      661 cgagtggatt tcgaaggaag ggtcgtgtcc tacgacttca acgaagtgga tgaactcatc
      721 ctcgcctacg cgatatccgt ccacaaatcc cagggaagcg agtaccccgc cgtggtgatg
      781 cccatcctga cgcagcacta ccttctcctg caaaggaacc tcctttacac ggcgatcact
      841 cggggcaaga aactggtcta tctcgtcggc accaaaaagg ccctctccat agcgatccgc
      901 aacgacaaac cccatagaag atacacactc ctcgcggaga gactcaggga gacaaaggca
      961 gtcccaggtc ccaggtctca cgtcccaggt taaagacaag aaaccggaga tgcctgcgtt
     1021 atccggtaag atcggaaccc ttgcaagaac catccgttct tggcctttga ccacgcaacc
     1081 cgcaacccgg aacccgcgac gatctctcct gtcatcatat attcactttc cccgtctacg
     1141 aaaacaggac atcggaatca tcgcttcggc gacagacagg tcgcgggaca ttccttaaga
     1201 ttgtacatat cccgggggaa aacaccgcgt gaccagcgga ggtggcccgg gcaaagaaga
     1261 tacggtgatg gtatgacgat cggaagaggg acgaaatacg cgggacgggc gcgctgtttc
     1321 tgtgcctggt gcggcaggga gcggaagagg aacggtgtct gggagaggtc agagttgaat
     1381 gcggagatat cacgcgtcgc ggtgacccat ggtatctgcc cggattgcgc ccgcgggctg
     1441 ctcggcggca gaacgaagga caaggcgatg aagagagcgg actgacgggg ggggaggcat
     1501 ttgtcgtaac aaacgatgac ggtcccgctg agaaagtacg acaagataat tatcgctgag
     1561 gcgacagaca tgaggtcatg tccgttgtag cgtactcgta gggaattctt cggttcctct
     1621 cgggagcgag gctccgcggg aggtttggat ctgtttatcc gggggagccg gatcatggcg
     1681 ggagaggtgg tggaacgatg tgtggaatag tcggatatga aggtacgcgg gaggcctgcg
     1741 atatcctcat cgacgcgttg agcaggcttg aatacagggg atacgattcg gcggggatag
     1801 cccttcttgg tcagggggag atccgcatag agagaaggaa ggggaaggtc gaagagctca
     1861 ggaaggtcct gaagggcgcg gctctcaagg ggagcatcgg actcgcccac acgcgctggg
     1921 cgacgcacgg ggtgccttcc gaaaggaacg cgcatcctca cagggcaggc gacgtggtgg
     1981 tcgtccataa cgggatcatc gagaactatc tcgagctgaa agagcacctg aaaggggccg
     2041 gccacgtttt catctccgat acggacacgg aggtcatccc ccatctcatt acggaccatg
     2101 cccggaaagg cagggatctc gttgactccg tccgcctggc cctcaaggaa ctcaaaggtt
     2161 cctgggcggt cgggatcctg aaggagtccg aaaggaccct ggtggccgca aagaaccaaa
     2221 gcccgctcat cgttggcgta ggtgacggag aatacttcat cgccagcgat ataccggcca
     2281 tcatcaacat gaccaacagg ttcatcttcc tcgaagacaa tgacatggcc atattcaaag
     2341 gccgcgatat gcggatcatc aatgtggacg gcgaggatgt cgtccgggag gtccggcagg
     2401 tatgctggac ggccggaatg gcggaaaaag gcggatacag tcacttcatg ttgaaagaga
     2461 tctttgagca gccccaggcg atatcggaca cactgctcgg aagggcgcgg gaggaagagg
     2521 gcgaggttga gttcgaaggg gtcgacctgc ctgaccctgc gggcatcagg aagatatgga
     2581 tggtggcgtg cggcacgtcc catcatgcct gcatgatcgg aaaaaatctg atcgagacga
     2641 gcctgagaat acccgtcgaa acggacatgg cctccgagtt cagatacagg gatcccatcg
     2701 tgtcgccccg cgacctcttc attgccgtct cccagtcggg ggagacggcg gacacgatcg
     2761 cggcgatgag ggaagcgagg tcccggggtg cttcaacgct gtcgatatgc aacgtcctcg
     2821 ggagcacgct ggcacgcgaa gccgaaaacg ttgttttcac gcatgccggc ccggagatcg
     2881 gcgtggcctc cacgaaggct tttaccacgc agatctccgt catgttcctg ctcacgctct
     2941 gcatcggtaa aagactgggc atcttcggcc cccaggatgt cagggagcgt cttcgcgaga
     3001 tccgggccat cccccgcaag gtgcgaagcg ttctcgatga ggcgccgtcg atacaggcgg
     3061 ccgccgcgag atacatgttt ttcaggagct tccttttcct gggaaggggc atcaacttcc
     3121 cgaccgcgct cgaaggggcc ctgaagctca aggagatctc ctatatacac gccgaggcgc
     3181 acgccgcggg tgaaatgaaa catggaccca tcgccctcat cgacgacgac atgccggtcg
     3241 tgtgcatctc tccggcggac cggacctaca agaaggcctg cggtaatatg gaagaggtga
     3301 tggcgcggaa gggcaacatc attctgatca gtgatgacga gacacatgag atggcgcccc
     3361 gggcggggga gatgatcacg gtcccccgca cggcgtacga gctgcagccg atcctctcca
     3421 tagtgcccct gcagttgctt gcgtactata tcgcggacct tctgggtatg gatgtcgacc
     3481 agccgcgcaa cctcgcgaaa agcgtgacgg tggagtgacg ccatgatcgg gagaagatat
     3541 caccaggcga gctcggaagg agaccgcggg ccatgatccg ggtgctgatc atcgatggcg
     3601 ggaggcagac gtgcgatatg ctggccgggt gtgcccttag cctggggtac gacgtgtcga
     3661 cgggacaaac ccttacggaa tgcctggaga aggtcacgtc caatcctccc gacgtgatct
     3721 tcatagatgg gcagctgccc gatggaagcc ccctggaggt ccttgatgtg atccacgccg
     3781 ctccgtcctc accgacggtc atcatcatga ccgagggcgg gacgccggag agcgccgaga
     3841 tggccatacg gaacggcgcc tgggactaca tcccgaagcc ggcctcgatg aaggagatga
     3901 cggcgcgcct gtcgcgtgtg gcccaatatc gcaggcaaca tcagggcagc gagcggatgg
     3961 cggagatcaa gcgcaacggc atcgtcggga acagtcccac gatgcggacc gcgatggaca
     4021 tgatcaaacg ggctgccgac agtgacctca atgttctcat cacgggggag acaggcacgg
     4081 gcaaggagct ctttgccctg tcgatccacc tgaacagcgg gaggtcccag gacaatttcg
     4141 tggtcgtcga ttgcacggcc cttcccgaaa ccctcgtgga gagcatactc ttcgggcatt
     4201 ccaagggtgc cttcaccggc gccgacaggg cgcaggacgg cctcatcagg caggcccacc
     4261 gggggacgct gttcctcgac gaaatgggag agttgccgct gccgctgcag aagacgttcc
     4321 ttcgcgtgct gcaggaacac cgcttcaggc ccgtcggaag tcagaaggaa gtggagagcg
     4381 atttcaggct tatatcggcg acgaacaaga acctggagca gatggtcgcg gaagggaaat
     4441 tcaggggcga cctcctctat cgtctcggcg ccatgacgat acacctgccc cccctgagac
     4501 agcatcccga agacataagg gagatggccg tcttttacgt ggagaaggtc tgtcgccgtt
     4561 tcggtgtcgc tccgaagaag ttctccgacg agttcctgaa atacctcata cggtacgatt
     4621 ggccggggaa cgtgagggaa ctgttccatg ttctcgagca cgcctgcgcg gccggcctcc
     4681 acgaggagac gctctttccc gtgcatctgc cgcaggagtt gcgcatcacc atcgcgagat
     4741 cgctgttcga ggataaccat cacgtcccgc gaaacggcgg caacggttcc ggatccagcg
     4801 cggcaaagca gatcctgagc ctgaagaggt ttcgccagag cgcctattcg gacctagagg
     4861 aaatgtacct caggaatctc ctgacgtcgt cggcgggcag catcaaggaa gcatgcgccc
     4921 tttccaaact gtcacggacg agactgtacg gtcttctcaa gaaatacaac ataaggcact
     4981 gagccccgcg tgacccgccc cgcgtcccgg aacccccggc gtcaccggtc atgccgccgg
     5041 agtgtaccgc aaagagtcca gttttattgg atgccgtccc ggaaaagtga acagatcttc
     5101 attccacgcc cccggtgatt ccgtcaacgg tacttcgtaa atcctttgct gtcaacgctt
     5161 tataaaagtg agggctggtg gcacggttat tgatatacgg tagtcattgt ggatgctgaa
     5221 cacacagaaa gaccgacggg acaacgcagt gcgaagaaga ggcgcccaag agtgcgaccg
     5281 ggggcagaga aacggtacga caccatcttc tacgccaaga atcgggagga ccttcccagg
     5341 aagctcagga gcgtgctgga accccttctc tttgacgagc acatcgagca cggcagggga
     5401 tatgaatgcc ggcagtttcg ggccgagggt gatgtctttt accccaagag agagcagggc
     5461 gagaagtacc aggactgctt tctgctcata ccaggcgatg ttgtatgaga ccggcagctc
     5521 gttgataccg acaccgaagg cctttgcgag ggcctgggcg atcaccacaa gggagtaact
     5581 gtcattgcac tggccggcat cgaggacccg
//
LOCUS       DAOQTR010000065         5587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_15971_length_5587_cov_3.844541, whole genome shotgun sequence.
ACCESSION   DAOQTR010000065 DAOQTR010000000
VERSION     DAOQTR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15971_length_5587_cov_3.844541"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKN85_02535"
     CDS             complement(<1..592)
                     /locus_tag="PKN85_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNT43306.1"
                     /translation="MNLKAIQKICYGVYIISSKNNGKLNGQIANTAFQIASDPPTLAI
                     SVNKQNLTHEYITSSKVFTVSILSKFAPMTLIGKFGFKSGRDIDKFADTGHRMGRTGA
                     PIVLDSTVGYLECEVLSMTDAGTHTVFIGKVTDCDVLSDAEPMTYAYYHTVKGGKSPK
                     AAPTYVQEDSRPAGPVETGKYVCNACGWVYDPAEGVP"
     gene            complement(624..1487)
                     /locus_tag="PKN85_02540"
     CDS             complement(624..1487)
                     /locus_tag="PKN85_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNT43307.1"
                     /translation="MEARDRYLGETPAWCPGCGNFSILNAFTAALEAEGIEPGGFCIV
                     SGIGQAGKFPHYVKCNTFNGLHGRALPVATGIKLANHELLVVAVGGDGDMYGEGGNHL
                     LHAMRRNIGVKVFVHDNQIYGLTKGQASPTSMEGMVTKNQPFGVLSEQLNPLAMAVAL
                     DCGFVARGYAGDREHLTELIRSAFHHDGLSLVDILQPCVSLNKVNTWEWYGKRVYRIG
                     EDHDLGDRVKAFEKALEWGDRIPIGVIYRNDRPSFEKRIPVIGDGPLVDGSGRWQELK
                     ERLSGSLKEFY"
     gene            complement(1487..3187)
                     /locus_tag="PKN85_02545"
     CDS             complement(1487..3187)
                     /locus_tag="PKN85_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNT43308.1"
                     /translation="MDYTIKIGGEAGQGIQTVGDALARVFSRLGCHVFTSQDYESRIR
                     GGHNFYQIRVSDRPVRAPRGPLDILVAFDRASITVHQGELVEQGIIVYDSSQLKESFS
                     GSQFLDVPFVKLATDAGAKPIAANTVATGAILGMLGFSIDPLYDIITALLKRKDNETR
                     EQNRRAAVAGYEYAAKNCGRCAFTLKPPGGAKMLMGGNEAIGLGALAADCRFYSAYPM
                     TPSTGIMLFLSSRAKDYGIIVEQAEDEIAAINMALGASFAGVRSMTGTSGGGFALMTE
                     GLSLAAMTETPIVIALAQRPGPATGLPTKTEQADLLFALNAGHGEFPRVIFAPGDPGQ
                     AVHLTAKAFDLSQKHQIPVFVMTDQYLADTQWTMDGFSVDGLPMRDWRMDGKEIDGVD
                     DYRRHAFTPSGVSPFAIPGKTRHVVVTDSDEHSEAGHIIEDGPTRKKMVEKRLFLKMD
                     SIRKDISPPLFLGPEEAETVLVSWGSTYGVVREVVEAMPERKMAMLHFSEVFPLPGLE
                     AFDYLDVLKRAKRTVSIEMNATGQFARLLRYETGYSVTDRVLRYDGRPFLVEELMEEL
                     "
     gene            3286..5427
                     /gene="rnr"
                     /locus_tag="PKN85_02550"
     CDS             3286..5427
                     /gene="rnr"
                     /locus_tag="PKN85_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNT43309.1"
                     /translation="MRKKGTAKQGKRDAEPITDAQVLRVLSSARRPVGIDELGDVFGR
                     GRPGRRDIMRHLRSLIAKGRVTELKNKRFGLTREMDLLSGTLWCTRGGNAFVIPDSGD
                     EKDVFVSSRNLNHAVHGDRVTVRLDHYRGKLEGKVIRILERKSPIIIGFTKIVDNVIY
                     VIPEDFRYQTKYLVSRPRREVRDGELVVARVTAFPGEKVEPECAITRVLGDLTSVAAI
                     GRFIEYKHSLSRRFPKHVEDEARSAVTSLTQRGRSDQRDLGFVTIDGKNARDFDDAVA
                     IERGRSGFTLHVAIADVSHYVKTGSLLDQEAGKRGMSVYFPDRVLPMLPKTLSNGICS
                     LNPLEERYTVTATIEFDRNGVVLGASFRPSLIRSAMRLTYEAVEKAVVENDGKTRQKI
                     EPCLAALTDMAELAGLITCTREKRGSIDFDLPEPEVILDIKGGIRQIVRSRRLYSHRI
                     IEEFMIAANEAVARYLEAKNVPALFRVHEPPEREKLRDFERLLQGLGIGHRKTDTSRA
                     LQDVLSTVKDTNYEFIVNRVLLRSMKQARYFSRNKGHYGLASGSYLHFTSPIRRYPDL
                     VCHRILKAALDGRQPVYDEEQLAAMAGHLSERERLVMEAERELEDRIRILYMKDRVGD
                     VFEGIISHVTSFGFFVELSEVFVEGLVLISSLGDDYYRFEEERFRITGRRTRKTYRVG
                     DAVTIRVVMADVATNRLHFEVAAGAGADKKL"
BASE COUNT         1139 a         1714 c         1599 g         1135 t
ORIGIN      
        1 ggggcactcc ttcagcggga tcgtagaccc agccgcaggc gttacacacg tacttccccg
       61 tctccaccgg acccgcaggc cgtgagtctt cctgcacgta cgtgggggca gccttcggag
      121 actttcctcc cttgaccgtg tggtagtacg cataggtcat gggctccgca tcgctcagga
      181 catcgcagtc ggtgaccttc ccgatgaaca ccgtgtgagt acccgcgtcg gtcatgctca
      241 ggacctcaca ttcgaggtaa cccacggtgc tgtcgagcac gatgggcgcg cccgttcttc
      301 ccatcctgtg tccggtatca gcgaacttat cgatgtccct gcccgacttg aacccaaact
      361 tgccgatgag cgtcatggga gcgaacttgg aaaggatcga taccgtgaac accttgctcg
      421 acgttatgta ttcgtgggtc aggttctgtt tgttgacact gatcgccagg gtcggcggat
      481 ccgacgcgat ctggaaggca gtgttggcta tctgcccgtt aagctttcca ttgttcttcg
      541 aactgatgat gtagacgccg tagcagatct tctgaattgc cttgagattc attccgttct
      601 cctttcagta cgctatgttg gcttcaatag aattctttga gagatcctga cagtctttcc
      661 ttcagctctt gccatctgcc tgacccgtcc acgagaggcc cgtcgcctat gacggggatc
      721 ctcttctcga aggagggacg gtcatttctg tatatcacgc cgatgggtat cctgtcgccc
      781 cactccaatg ccttctcgaa agccttgacc ctgtcaccga gatcgtggtc ctccccgatc
      841 cggtagaccc ttttcccgta ccattcccag gtgttgacct tgttcaggga aacgcagggc
      901 tggagtatgt cgacaagaga taacccgtcg tggtggaagg ccgagcggat gagctccgtg
      961 agatgctcgc ggtctcccgc gtatcccctc gccacaaaac cgcagtccag cgccaccgcc
     1021 atggccaggg ggttgagctg ctccgacagg acaccgaagg gttggttctt ggtcaccatc
     1081 ccctccatgc tcgtcggcga ggcctggccc ttcgtgagcc cgtagatctg gttgtcgtgc
     1141 acaaagacct ttacgccgat gttgcgcctc atggcatgga gaaggtgatt gcctccctcg
     1201 ccatacatgt cgccgtcccc gccgacggcc acgaccagca gctcgtggtt ggcaagcttg
     1261 atccctgtgg caacgggaag cgcccttccg tggagtccgt taaaggtgtt gcatttcaca
     1321 taatgaggaa atttccccgc ctgaccgata cctgaaacga tgcagaaccc gccaggctcg
     1381 atgccttcgg cctcaagggc cgccgtgaag gcgttcagaa tggaaaagtt cccgcaaccg
     1441 ggacaccatg ccggagtctc ccccaggtac ctgtcgcgcg cttccatcag agctcctcca
     1501 tcagttcctc cacgaggaag ggtcttccgt cgtacctcag gaccctgtcc gtgacggaat
     1561 accccgtctc gtatctcaac aaccgggcga actgccctgt ggcgttcatc tctatggaaa
     1621 ccgtcctttt ggcacgcttc agcacgtcaa gataatcgaa ggcctcgaga ccgggcaacg
     1681 ggaagacctc actgaaatga agcatcgcca tcttcctctc cggcatggct tccaccactt
     1741 ccctgacgac accatatgtg gacccccagg atacaagaac agtctccgcc tcctccgggc
     1801 ccaggaacag gggaggtgag atgtctttcc ttatgctgtc catcttgagg aagagtctct
     1861 tctccaccat cttcttccgc gtcggcccgt cctcgatgat gtgccccgcc tcgctgtgtt
     1921 cgtcgctatc ggtgacgacg acgtgcctcg tctttcccgg tatggcgaag ggcgaaaccc
     1981 ccgaaggggt gaacgcgtgt cttcggtagt catcgacgcc gtcgatctcc ttgccatcca
     2041 tacgccaatc gcgcatcgga agaccgtcca cggagaagcc gtccatcgtc cattgcgtgt
     2101 ccgcgaggta ctggtcggtc atcacaaaga cggggatctg gtgcttctgg gaaaggtcga
     2161 aggccttcgc cgtcagatgc actgcctgcc ccgggtcgcc cggggcgaat atgacgcggg
     2221 gaaattcgcc gtggccggcg ttgagggcga acaggagatc ggcctgttcg gtcttcgtcg
     2281 gcaaccccgt tgccggcccc ggtctctggg caagggcgat gacgatgggg gtctccgtca
     2341 tggccgcgag ggaaagcccc tcggtcatga gggcgaaacc gccccccgat gttcccgtca
     2401 tcgatctgac cccggcaaag gaagctccga gcgccatgtt gatggccgct atctcgtctt
     2461 ccgcctgttc cacgataatg ccgtaatcct ttgccctgga gctgaggaag agcattatgc
     2521 ccgtggatgg cgtcatggga taggcgctgt agaacctgca gtccgccgcc agggcgccga
     2581 gaccgatggc ctcgttcccg cccatgagca tcttcgcacc gccgggcggc ttaagcgtga
     2641 aggcgcaacg tccgcagttc ttcgccgcgt attcatagcc ggccacggcg gcgcgcctgt
     2701 tctgctcccg cgtctcattg tcctttcttt tcaggagggc ggtgatgatg tcgtagagcg
     2761 ggtcgatgga aaagcccagc attcccagga tggcccccgt ggccaccgtg ttggccgcga
     2821 tcggcttcgc ccccgcatcg gttgcaagct tcacgaacgg aacatccaag aattgagacc
     2881 cgctgaaact ctccttcaac tgggaggaat cgtatacgat gatcccctgt tccacgagct
     2941 cgccctgatg gaccgtgatg gacgcccggt cgaaggccac caggatgtcg aggggcccgc
     3001 gcggcgcgcg gacgggccgg tcgctcacac ggatctgata gaagttgtgc cccccgcgga
     3061 tgcgcgactc gtagtcctgg ctcgtgaaaa catggcatcc cagcctggag aacaccctcg
     3121 ccagggcatc gcccaccgtc tggatcccct gtcccgcctc gccgcctatc ttgatcgtat
     3181 agtccatttc gctcagaaca acattatagt gggttgcctg gcaaatatca accgctcctt
     3241 cctgccgagg attggaaaca ttgaataaac gtgatataac ctatcatgcg gaaaaagggg
     3301 actgccaaac agggaaagag ggatgccgag cccatcaccg atgcccaggt ccttcgggtg
     3361 ctgtccagtg cgcgacgacc cgtaggcatc gatgagttgg gagatgtttt cgggcgtggc
     3421 agacccggac gccgggatat catgcgtcac cttcgctcgc ttatagcaaa gggccgcgtg
     3481 acggagctga agaacaagag gttcggtctc accagggaga tggacctcct ttccggaacc
     3541 ctgtggtgca cccgcggcgg caacgccttc gtcatacccg attcagggga tgagaaagac
     3601 gtcttcgtgt cgtcccggaa cctcaatcac gccgttcatg gcgaccgcgt caccgtgaga
     3661 ctcgaccact atcgggggaa gctggaagga aaggtcatca ggatactgga gagaaaatca
     3721 cctattatca taggcttcac aaagatagta gacaatgtaa tttatgttat tccggaagac
     3781 ttccgatatc aaacaaaata tcttgtatca cgaccacgca gggaagtccg ggacggggaa
     3841 ctggtggtcg cccgggtaac agcctttccc ggcgaaaagg tcgagcccga atgcgccatc
     3901 accagggttc tcggcgacct cacgagcgtc gccgccatcg ggaggttcat cgaatacaag
     3961 cactcccttt cccgtcgctt cccgaaacat gtcgaggacg aggcgcgctc cgccgtgact
     4021 tcccttactc agagaggccg ctcggaccag agggaccttg gattcgtcac catcgacggg
     4081 aagaacgcgc gggatttcga tgacgccgtc gccatcgagc gcgggagatc ggggttcact
     4141 ctccatgtgg ccatcgccga tgtctcccac tacgtgaaga ccggctcatt gctggaccag
     4201 gaggccggaa agcgagggat gagcgtctat tttcccgacc gggtgctccc gatgcttccc
     4261 aagacgctct ccaatgggat ctgcagtctc aatcccctcg aggaaaggta taccgtcacg
     4321 gccaccatcg agttcgacag gaacggcgtg gtgctcggcg cctcctttcg accttccctg
     4381 atacgcagcg ccatgcgcct cacctatgaa gccgtcgaga aggccgtcgt cgagaatgac
     4441 gggaagacgc ggcagaagat cgaaccctgc ctcgcggccc tgaccgacat ggccgaactc
     4501 gccgggctta tcacctgcac ccgggaaaag aggggaagca tcgatttcga ccttcccgaa
     4561 cccgaggtca tcctcgacat caagggcggc atccgccaga tcgtccggtc ccggcgtctc
     4621 tattcacacc gcatcatcga ggagttcatg atagcggcca acgaggccgt ggcgcgctat
     4681 ctcgaagcga agaacgtccc ggcgctcttc agggtccacg aacccccgga aagggagaag
     4741 ctccgcgact tcgaacgtct cctccagggg ctgggcatcg ggcacaggaa aaccgacact
     4801 tcccgggccc tgcaggacgt gctgagcacc gtgaaggaca ccaattacga gttcatagtc
     4861 aacagggtcc tgttgaggtc gatgaaacag gcccgttact tctcccggaa caaggggcat
     4921 tacggccttg cctccggcag ctacctccat ttcacatcgc cgatacgcag gtaccccgac
     4981 cttgtctgtc acaggatact gaaggctgcc ctggacggca gacagcccgt ctacgacgaa
     5041 gagcaacttg ccgcgatggc cggtcacctg tctgagcggg agcgactggt catggaggcc
     5101 gaacgggaac tcgaggacag gatccggata ctctacatga aggacagggt cggtgatgtc
     5161 ttcgaaggca tcatctccca cgtcacttcc ttcgggttct tcgtcgagct ttccgaggtg
     5221 ttcgtggagg gtctcgtcct catctcgagc cttggcgacg actattaccg tttcgaggag
     5281 gagcgtttca ggatcacggg gagaaggacg aggaagacct atcgcgtcgg tgacgccgtg
     5341 acgatacggg tcgtcatggc ggatgtggcc accaacaggc tccatttcga ggtggcagcc
     5401 ggcgcgggcg ccgacaaaaa gctttagctt gtatcggatc ctgatgataa tatagtacga
     5461 caagaacatc cttgagagac gaccatgccc gcggaagaca aacttagatc ctcaaagcac
     5521 gcggggcgcc gcaagggacg acccgaaaaa aagccttccg tcgcgtcggg caggaataca
     5581 ccaaaga
//
LOCUS       DAOQTR010000066         5543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16153_length_5543_cov_2.867165, whole genome shotgun sequence.
ACCESSION   DAOQTR010000066 DAOQTR010000000
VERSION     DAOQTR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16153_length_5543_cov_2.867165"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PKN85_02555"
     CDS             complement(<1..733)
                     /locus_tag="PKN85_02555"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HNT43310.1"
                     /translation="MKAFVALEDGTVFEGRGFGLEGEKEGEIVFNTSMTGYQEIMTDP
                     SYKGQIVTMTYPLIGNYGVNDEDVESGGPKVEGFIAREFSRITSNWRATGDLGDYFRK
                     HNIIAVDEIDTRALTRHLRVRGVMRGIISTTDHDRDSLVRKAQGSPGIVGVDLVSGVT
                     CQAPYEFEGGPGGDPAVDALHCVVMDFGVKRNILRILRQKGCRVTVVPAGTKAEDIVA
                     MGPDGVLLSNGPGDPEAVPYAIREIE"
     gene            complement(730..1416)
                     /locus_tag="PKN85_02560"
     CDS             complement(730..1416)
                     /locus_tag="PKN85_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HNT43311.1"
                     /translation="MTGEEGRAGHNEKIEGWLRGLDVDTFGIADMSCYGEEITGIGDV
                     ATNELPFAVSFGIVLSVGVLSTLKDGPTQLYLHHYRQLNYRLDMIGYFLGRSIEREGF
                     RAIPFAASQVVDWQRQRGHINHKKVGVMAGLGWIGRNNLLVHPLFGSQVRYNTVLTDM
                     PLEAGRELGRDCGSCTACVAACPAGAIRTERSEFDHHGCYEMLSRFRKERNIGHHICG
                     VCVKICGGKR"
     gene            complement(1413..3275)
                     /gene="mnmG"
                     /locus_tag="PKN85_02565"
     CDS             complement(1413..3275)
                     /gene="mnmG"
                     /locus_tag="PKN85_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNT43312.1"
                     /translation="MDSYDIIVVGAGHAGCEAALAAARMGARTLLLTINLDHIAYMSC
                     NPAVGGLGKGHLVREIDALDGEMGVNTDEAGIQFKVLNMKKGPAVRATRIQTDMALYA
                     RRMKRRLEETEGLSIRQGVARNVLIEKGAAAGVETMLGERLAAPAVIVTTGTFLKGLI
                     HVGLEHFEGGRLGDMPSVGLSDNLRELGFQLGRLKTGTCPRLDGRTIDFSKMVPQPSD
                     DPPVPFSFMTGEVKNRLVPCYLAYTNERTHDVIRTGFDRSPLFTGVIKGTGVRYCPSI
                     EDKIVRFSDRSRHRVFIEPEGLDTVEYYPNGLSTSLPLDIQVAMLRTVEGLEEVRITR
                     PGYGIEYDFAYPTQLYPTLETKLVPNLYFAGQINGTTGYEEAAAQGLMAGINAVLRLR
                     GEGDLVLGRHEAYIGVLIDDLVTKGVDEPYRMFTSRAEHRLLLREDNADLRLTGKGHG
                     IGVVGDGRCARVLEKKRKVGEAFSLLRGVRLRPTSKTQEMLREKGIEAITNPLTLEEL
                     LKRPDVSFDDLKGIHEGLRDIDSDIAYQVEVEVKYRGYTDRQLELVARTSKLDDRRIP
                     RELDYAGVSGLSREVVERFVKVRPSTLGQASRIPGVTPASITALLVHFKKKGML"
     gene            3415..3669
                     /locus_tag="PKN85_02570"
     CDS             3415..3669
                     /locus_tag="PKN85_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43313.1"
                     /translation="MEERAIGVVVRYFAKIGVAAIRITEGELKVGDRIRIKGHSTDLE
                     EEIESMQVEHENVQMVGAGTDVGIKVKERVREHDVIYLLT"
     gene            3808..4392
                     /locus_tag="PKN85_02575"
     CDS             3808..4392
                     /locus_tag="PKN85_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43314.1"
                     /translation="MEDKELKQRFLDIFEAATPILEDLKKGFFTQQVGLIQECEKKFG
                     DLLISRVPYVEKIIGEKEKSEADKKYVRLLPALQTIALAIENLMYKMEAKVAHNILFS
                     EKALSEVRDLYSLMQEQFRDTKDYVHTKNPGLHQAVKQHMEKMFRMIEELALVHQNRL
                     ITGICMPQASYLYLDITDSMKRITRGLVDFADRV"
     gene            4492..>5543
                     /locus_tag="PKN85_02580"
     CDS             4492..>5543
                     /locus_tag="PKN85_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT43315.1"
                     /translation="MGGAIREIALGREPRDWDLALSDADDLARLEEIFGRRSFLLGKK
                     PIQTHRLVAGETVIDITILEESIEKDLRRRDFTMNAVAFDIRRDRVIDPLSGLHDIER
                     RLIRYPDRDTIASDPLRMLKAARHYAALTGFTLHPELIEAMASSRDLIRKTAPERIKY
                     ELDLIMISQGVHRGMEILTRTGLIFEIVPELEALRVMDVEKNFSLETFGHTLLGFSFL
                     HDRGGRYLANRAAAKDVGYALLFHDLGKAHTYSYDEVKGLVHFFNHERRSQEMASAIM
                     ERLRFSTHEMKRISALIEHHMRIFLISSAEATEKAIRRLIYRLGDLTPELIALTLCDM
                     YGSSGGEENPSTRQVV"
BASE COUNT         1111 a         1724 c         1575 g         1133 t
ORIGIN      
        1 tctctatctc cctgatggcg tagggaaccg cctcggggtc tcccggtccg ttggagagaa
       61 ggaccccgtc gggccccatg gcgacgatat cctccgcctt cgtgcccgcc ggcacgacgg
      121 tgacgcgaca gcccttctgg cgcaatatcc tcaggatgtt cctcttgacg ccgaaatcca
      181 tgacgacgca gtggagcgcg tcgacggccg ggtcgccccc gggtccgcct tcgaactcat
      241 agggcgcctg acaggtcacg ccgctcacga ggtcgacacc aactatcccc ggcgaaccct
      301 gggcctttct cacgagactg tcgcggtcgt ggtccgtggt ggatatgatg cccctcatga
      361 cgccgcgcac cctgaggtgg cgggtgagag ctctcgtatc tatctcatcg acagcgatga
      421 tattgtgctt gcggaaataa tcgccgaggt cgcccgtcgc tctccagttg ctcgttatcc
      481 gggagaactc ccgcgcgatg aagccttcca ccttgggccc gccggattcg acatcctcat
      541 cgttgacgcc gtagttgccg atcagcggat aggtcatcgt gacgatctgt cccttgtagg
      601 agggatcggt catgatctcc tggtacccgg tcatgctcgt gttgaagacg atctctccct
      661 ctttctctcc ctcaaggccg aaacctcttc cttcgaagac cgtcccatcc tcgagcgcca
      721 cgaaggcctt catcgcttcc ctccgcaaat cttcacgcag acaccgcata tgtgatggcc
      781 gatgttgcgc tcttttctga acctgctcag catctcatag cacccgtggt ggtcgaattc
      841 cgacctctcc gtcctgatgg cccccgcggg gcatgccgcg acgcaggccg tgcagcttcc
      901 acaatcccgg cccagttcgc ggcccgcctc gaggggcatg tccgtgagga ccgtgttgta
      961 cctgacctgc gacccgaaca gggggtgaac gagcaggttg ttgcgtccga tccatccgag
     1021 acctgccatg actcccacct tcttgtggtt gatgtgaccc cgctggcgct gccagtccac
     1081 cacctgggat gcggcgaagg ggatcgccct gaacccttcc ctttctatcg atctgccgag
     1141 aaaatatccg atcatgtcta gcctgtagtt gagctggcga tagtggtgga ggtacaactg
     1201 ggtcggaccg tccttaaggg tgctgagcac ccctaccgag agtacaatgc caaaagagac
     1261 ggcaaatggt aactcatttg tcgcgacgtc gccgataccc gttatctcct cgccatagca
     1321 ggacatatcg gcgatcccga aggtatcgac atcgaggccc cggagccatc cttcgatctt
     1381 ctcgttatgc cctgcccttc cctcttctcc tgtcatagca ttcccttctt cttgaaatgg
     1441 acaagcagcg ccgtgatgga agcgggggtg acccccggga tccgcgacgc ctggccgagc
     1501 gtcgacggcc tgaccttcac gaaacgttcg accacctccc gagaaagccc cgatactccg
     1561 gcataatcca gttccctcgg tatccgcctg tcatcgagct tgcttgtcct ggcgacgagt
     1621 tcgagctggc ggtcggtgta gccgcggtat ttcacctcaa cttccacctg gtaggcgatg
     1681 tcgctgtcga tgtcccgcaa tccctcgtga atgcccttga gatcatcaaa ggagacgtcc
     1741 ggcctcttga ggagctcctc gagggtcagc gggttcgtga tggcctcgat ccccttctcc
     1801 cggagcatct cctgcgtctt actcgtgggc ctcagcctca ctcccctgag aagcgagaag
     1861 gcctctccga ccttcctctt cttctccagc acccgcgcgc agcgcccgtc gcccaccaca
     1921 ccgatgccgt gccccttccc cgtcagccta aggtccgcgt tgtcttccct cagcaggagc
     1981 cggtgctccg cccgggacgt gaacatccgg tagggctcgt cgacgccctt cgttacgagg
     2041 tcatctatga ggacaccgat gtaagcctcg tgccggccaa gcacgagatc tccctctccc
     2101 cggagcctga gcacggcatt gatgcccgcc atgagtccct gcgcggcggc ctcttcgtag
     2161 cccgtggtcc cgtttatctg gcccgcgaag tagagattgg gtacaagctt cgtctccagt
     2221 gtcggataaa gctgggtggg gtaggcaaaa tcgtactcta tcccgtaacc cggccgggtt
     2281 atccgcacct cttcgaggcc ttcgacggtc ctgagcatcg cgacctggat atcgaggggg
     2341 aggctcgtcg acaaaccgtt gggatagtac tcgaccgtgt cgagcccctc cggctcgatg
     2401 aagacacggt gcctcgacct gtcgctgaac ctcactatct tgtcctctat ggatggacag
     2461 tacctgaccc ccgttccctt gatgacgccc gtgaagaggg gggaacgatc gaaacccgtc
     2521 cggatgacgt cgtgcgtcct ctcgttcgtg tacgcgaggt agcagggaac gagcctgttc
     2581 ttcacctccc cggtcatgaa agagaagggg acgggcgggt cgtcgctcgg ctgcgggacc
     2641 atctttgaga aatcgatcgt cctgccgtcg agtctcggac aggtgccggt cttcagccga
     2701 ccaagctgga aaccgagctc ccgcaggttg tccgacaggc ccacagaggg catgtcgccc
     2761 agccttcccc cctcgaaatg ctcgaggccg acatggatga gccccttgag aaaggtcccc
     2821 gtcgtcacga tgaccgccgg cgccgcgaga cgttccccga gcatcgtctc cactccggcg
     2881 gcggcgcctt tttcgatgag cacattccgg gccactccct ggcgtatcga aagaccctcg
     2941 gtctcctcaa gccttctctt cattctccgg gcatagaggg ccatgtccgt ctgtatcctc
     3001 gtcgcccgga cggcggggcc cttcttcatg ttgaggacct tgaactggat gcccgcctca
     3061 tcggtattga cacccatttc cccgtccagg gcatcgatct ccctgacgag atggccctta
     3121 ccgagaccgc cgacggcagg gttgcaggac atgtaggcga tatggtcgag gttgatggta
     3181 agaagaaggg tccgcgcgcc catccgagct gcggcaagcg ccgcctcgca tccggcatgt
     3241 ccggccccca ctacgatgat atcatacgaa tccatatgca acgtccttaa aagccgtata
     3301 ttgtagcagg aatcgagcct cttttccacc cgcttcagga ataatgcatt atcactgttc
     3361 aattattctt atccacatgg taggatggaa gaaaaaggtg acagggaggg tcgcatggaa
     3421 gaaagggcca tcggtgtggt tgtcaggtat tttgccaaga tcggggttgc ggccataagg
     3481 atcaccgaag gggagttgaa ggtcggtgac cgcatcagga taaaaggcca ttccacggac
     3541 ctggaagagg agatcgagtc gatgcaggtg gagcacgaga acgtccagat ggtcggcgcg
     3601 ggcaccgatg tggggatcaa ggtcaaggaa agggtgcgcg agcacgacgt catctacctc
     3661 ctgacataga caggtcgcac aaggcggacg ccagagggcc gtccttgccg accgccggca
     3721 gaggctgctt ttttgctctt cagggctgac agacagaccc ttctgtgtta taagatacgg
     3781 aaaagatctc acggaaatgg aggagcgatg gaagacaaag agctgaagca gcgtttcctg
     3841 gatattttcg aggcggcgac ccccattctt gaagacctga agaaagggtt cttcacccag
     3901 caggtggggc tcatacagga atgcgagaag aagttcggcg acctgctcat ctcgcgcgtt
     3961 ccctacgtcg aaaagatcat cggggaaaag gaaaagagcg aggcggacaa gaagtacgtg
     4021 cggctgcttc ccgccctgca gaccatcgcc ctggccatcg agaacctcat gtacaagatg
     4081 gaggcaaagg tcgctcacaa catccttttc agcgaaaagg cgctttccga ggtgcgtgac
     4141 ctgtatagcc tcatgcagga gcagttccgc gacacgaagg actacgtgca cacgaagaat
     4201 ccgggtcttc accaggcggt caaacagcac atggagaaga tgttcaggat gatcgaagag
     4261 cttgccctgg tccatcagaa caggctcatc acggggatat gtatgcccca ggcatcctac
     4321 ctctacctcg acatcaccga ttccatgaag aggatcaccc ggggactcgt ggatttcgcc
     4381 gacagggttt agtggttggg aatctctcct caggaggttg cccgttcgat caccgggggt
     4441 gacgttggag gcagactcgc tgcatgcccc ttcagggggg aggtttttct cgtgggagga
     4501 gccatacggg agatcgccct cggcagggaa ccccgggatt gggacctcgc cctatccgat
     4561 gccgacgatc tcgcgcgcct cgaggagatc ttcggcaggc ggtcctttct ccttggaaag
     4621 aaacccatac agacacaccg gctggtcgcc ggagaaaccg tcatcgacat caccattctc
     4681 gaggagagca tcgagaaaga cctccggaga cgggacttca ccatgaacgc cgtcgccttc
     4741 gatatcaggc gggaccgggt catagaccct ttgagcgggc ttcatgatat cgaaagacgg
     4801 cttatccgtt accccgaccg cgacacgatc gccagcgacc ccttgaggat gctcaaggcc
     4861 gcaaggcatt acgccgccct cacgggcttc acgctccatc ccgagctcat cgaggcaatg
     4921 gcctcttcgc gagatctcat tcgaaagacg gcgccggaga ggatcaagta cgagctggac
     4981 cttatcatga tctcccaggg cgtccatcgc gggatggaga tcctcacgag aacgggcctg
     5041 atcttcgaga tcgttcccga gcttgaggcg ctgcgcgtca tggacgtgga gaagaacttc
     5101 tcactcgaga ccttcggtca tacccttctg ggattctcct tcctccatga ccgcggcggc
     5161 cgttacctgg cgaaccgggc agccgcgaaa gatgtcggct atgccctgct cttccacgac
     5221 ctgggaaagg cccacaccta ttcttatgac gaggtcaagg gtctcgtcca tttcttcaat
     5281 cacgagcgcc ggtctcagga gatggcctcg gccatcatgg agcggctgcg tttcagcacc
     5341 catgagatga aacggatatc ggccctcata gaacaccata tgcgcatctt tctcatcagc
     5401 tccgcggagg ccacggaaaa ggcaataaga cgcctcatct acaggctggg cgacctcacc
     5461 ccggagctca tagccctcac tctctgcgac atgtatggaa gctcgggagg ggaagagaac
     5521 ccctcgacgc gccaggtcgt tca
//
LOCUS       DAOQTR010000067         5536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16176_length_5536_cov_3.693851, whole genome shotgun sequence.
ACCESSION   DAOQTR010000067 DAOQTR010000000
VERSION     DAOQTR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16176_length_5536_cov_3.693851"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1669)
                     /gene="uvrC"
                     /locus_tag="PKN85_02585"
     CDS             complement(68..1669)
                     /gene="uvrC"
                     /locus_tag="PKN85_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNT43316.1"
                     /translation="MTPGIRTDGLPESPGVYLFKDHDDRIIYIGKARNIRDRVRSYFR
                     DDVKDIKTRTLAETIHAVDFILTANEKEAFLLENNLIKQHVPKYNIDLKDDKSYISLQ
                     LTIKDEYPALFATRKIVDDGSLYFGPYPHARDVRDILKIIERLYPVRKCKMTVFRKRK
                     RPCMLYELGKCPGPCVATVDATEYAGTINEVRDFLSGRDEKVLKDIEGRIAKKVAAWD
                     FEGAQALKERHQAIKQMTQKQHVHEHFGKNRDAWAFSEGTGRVSLVVLTFRRGVLLSR
                     RLYKERFYGEGPGEAIMTFLFQYYSSRPIPDEIILSEDMEDSDVLEKYLTERKKGPVR
                     IMGPSHSGARDMVRLAVENLHEPEVLPLDESFRNALRLKAAPRRIEIYDISHIGGQNP
                     TGAFTVFKDFKPAKDQYRVFHVRSDQTMDDVAMITEVLTRRVKNTDLGPLPDLFIIDG
                     GKGQLAAAHRVLKANNVDRDIISIAKGERRRKMEDVIYVPARKNPLALPRASAVFKEI
                     VKMRDEAHRFAVASHRRWRRKENLK"
     gene            complement(1666..3654)
                     /gene="uvrB"
                     /locus_tag="PKN85_02590"
     CDS             complement(1666..3654)
                     /gene="uvrB"
                     /locus_tag="PKN85_02590"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNT43317.1"
                     /translation="MKRFKLVSDYEAKGDQPAAIEKIAGNIFQGVAHQVLLGVTGSGK
                     TFTMANVIEKVNRPTLVISHNKTLAAQLYAEFKTLFPDNEVHFFVSYYDYYQPEAYIP
                     STDTFIEKDSSINEEIDRMRLMATNALFEREDIIIVASVSCIYGLGSPETYYGMLLTI
                     EEGQVIERQAILQRLVQGQYERNDVDFYRGRFRVRGGTIDVFPPYEEDRAFRIIHDED
                     RVVSISGIDPLTGKALERFRRVIIFPTTHYVMPRQTIDRAIASIEEELAAHLGLLRSH
                     NKLLEAQRLEMRTRYDLEMLQELGYCSGIENYSRHFTGRAPGEPPPTLMDYLPANALV
                     MIDESHVSIPQLAAMYRGDRSRKTTLVEHGFRLPSALDNRPLTFEEFEAREKQVLYIS
                     ATPAQYELVRSRGCVAEQIIRPTGLMDPEIILKPATDQVENLIVEIQKTTQAKERVLV
                     TTLTKRFAEDLTDFLIDRGIKTKYLHSDVDTLERVAIVRDLRLGKFDVLVGVNLLREG
                     LDLPEVSLVAILDADKEGFLRSQTSLIQTCGRAARNLNGRVIMFADNITQSMRRAIDE
                     TERRRAVQSAYNEENGITPEGIRKSIVDVLSSIYEKDYYEVPVDELDAKGLKPKQLSK
                     MVKKLKKEIAEAAKKWDFEKAALLRDRLLAIEKTELTL"
     gene            4001..4582
                     /locus_tag="PKN85_02595"
     CDS             4001..4582
                     /locus_tag="PKN85_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43318.1"
                     /translation="MKKVLLFALVMLISIAFVGAGFAQEKAKAPEKAKPAAEKPAVEK
                     PAPAKEEKPAEAKEPEKAAEKPKPKPMAGFVGKVTKTDSVFKTICVQRGKDEVAFDVS
                     EAKLKGYKNLDEINVGDKVAVQYKKGAMTLIKIAGAKKAKAAKKDAVAKPKKEKKEAA
                     TDEQKPAEEKKPAKKAKKAMKDEKKEEKKDEKK"
     gene            complement(4981..5316)
                     /locus_tag="PKN85_02600"
     CDS             complement(4981..5316)
                     /locus_tag="PKN85_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNT43319.1"
                     /translation="MKKVVSILMLSGLILVLSCGFVLAAEEAAAKAPLDSNVKAMLAI
                     GAGAAIGIAALGGALGQGKAAAAALEGIARNPGASGKIFAPMIIGLALIESLVIYALV
                     IAFIFVGKL"
BASE COUNT         1158 a         1438 c         1708 g         1232 t
ORIGIN      
        1 gttcactcgt taggcgcgtg agcgccgacc cgtttgcacg aagtgcagac ccgtgtgcgt
       61 cttttcccta tttcaaattc tccttccttc tccaccttct gtgcgaggcc acggcgaagc
      121 ggtgtgcttc gtcgcgcatt ttcacgatct ccttgaagac ggctgacgcc cggggcaggg
      181 cgagggggtt ctttctggcc gggacgtaga tgacgtcttc catttttctg cggcgctctc
      241 cttttgctat ggagatgatg tccctgtcga cgttgttggc cttgagaacc cggtgggccg
      301 ccgcgagctg tcccttgccc ccgtctatta tgaaaaggtc cggcaggggg ccgagatcgg
      361 tgttcttcac ccggcgcgtg agcacctccg ttatcatggc cacgtcatcc atggtctggt
      421 ctgaccggac gtggaaaacg cggtactggt ccttcgctgg cttgaaatcc ttgaagacag
      481 tgaacgctcc cgtcgggttc tgtccgccga tatgcgagat gtcgtatatc tcgatgcgcc
      541 gcggcgccgc cttcaggcgg agggcgttcc ggaaggactc gtcgaggggc aggacctccg
      601 gctcgtggag gttctccacc gcgaggcgca ccatgtccct cgcgccactg tgggaaggcc
      661 ccatgatcct cacggggccc ttcttccttt ccgtgaggta cttctccagg acgtcgctgt
      721 cctccatatc ttccgagagg atgatctcgt cgggtatggg ccgcgaactg tagtactgga
      781 agagaaaggt cattatcgcc tctcccggac cttccccgta gaaacgttcc ttgtacaggc
      841 gccgcgagag aagaacgccc cggcggaagg tgagcacgac gagggaaacc ctccccgtgc
      901 cctcggagaa ggcccaggcg tcgcggttct ttccgaagtg ctcgtggaca tgctgcttct
      961 gcgtcatctg tttgatggct tgatggcgct ccttcagggc ctgagccccc tcgaaatccc
     1021 aggcggcgac ctttttcgct atgcgtccct cgatgtcttt caggaccttt tcatccctcc
     1081 cggagaggaa gtcccgtact tcattgatgg tgcccgcgta ctcggttgcg tcgacggttg
     1141 ccacgcaggg ccccgggcac ttgcccagtt cgtagagcat gcagggtctc ttcctcttgc
     1201 ggaagacggt catcttgcat ttcctgacgg gatagagccg ctcgatgatc ttcaggatgt
     1261 cgcggacatc gcgggcgtgg gggtaaggcc cgaagtagag cgagccgtcg tcgacgatct
     1321 tccgcgtcgc gaagagggcg gggtactcgt ccttaatggt gagctgcagg gatatgtacg
     1381 atttgtcgtc cttcaggtca atgttgtatt tggggacgtg ctgtttgatg aggttgttct
     1441 cgaggagaaa ggcctctttc tcgttcgctg tgaggatgaa gtccacggca tggatcgtct
     1501 ccgccagtgt ccgtgtcttg atgtccttca cgtcgtcgcg aaagtagctg cggacgcggt
     1561 cgcggatgtt cctggccttg cctatgtaga tgatcctgtc gtcgtggtcc ttgaagaggt
     1621 agacacccgg tgattcgggc aggccgtcgg tcctgatccc gggcgtcaca gggtgagctc
     1681 cgtcttctca atggccagga gcctgtcgcg gagcagcgcc gctttctcga agtcccattt
     1741 ctttgcagcc tcggctatct ctttcttcag ttttttgacc attttcgaaa gctgtttggg
     1801 tttgagtccc ttcgcgtcca gttcgtccac gggcacctcg tagtagtcct tctcgtagat
     1861 ggacgagagg acatcgacga tggacttgcg gatcccctcg ggggttatac cgttctcctc
     1921 gttgtaggcg ctctggacgg cacgccgccg ctcggtctcg tcgatggccc ggcgcatgga
     1981 ttgcgttatg ttgtccgcga acatgatgac cctgccgttg aggttgcggg ccgcccggcc
     2041 gcaggtctgt atgagagagg tctgggaacg caggaagcct tccttgtcgg cgtcgagtat
     2101 ggcgacaagg gagacctcgg gaagatcgag gccttcccgc aggaggttca cgccgacgag
     2161 aacgtcgaac tttcccagtc tcaggtccct cacgattgcc accctctcga gggtgtccac
     2221 gtcggaatgg aggtactttg tcttgatgcc cctgtcgatg aggaagtccg tgaggtcctc
     2281 ggcaaaacgc ttggtgagcg tcgtgacgag gacccgctcc tttgcctgag tggtcttctg
     2341 aatctcgacg atgaggttct ccacctggtc cgttgccggt ttgaggatga tctcggggtc
     2401 catgaggcct gtgggccgga tgatctgctc ggcaacgcag ccccgggacc tcacgagttc
     2461 gtattgagcg ggggtggcgg atatgtaaag gacctgcttt tccctcgcct cgaactcctc
     2521 gaaggtgaga gggcggttgt ccagagccga aggcaggcgg aacccatgct ccacgagggt
     2581 tgtcttgcgt gaccggtctc cgcggtacat ggccgcgagc tggggtatcg agacatggct
     2641 ctcgtcgatc atcacgaggg cgttcgccgg gagataatcc atgagggtgg gagggggttc
     2701 cccgggagcc ctgcccgtga agtggcggga atagttctcg atccccgagc agtacccgag
     2761 ttcctgaagc atttcgaggt cgtacctggt cctcatttcc aggcgctgag cctcgaggag
     2821 cttgttgtgg gacctcagaa ggccgaggtg ggcggcgagt tcctcctcga tggaagcaat
     2881 ggcccggtct atcgtctggc ggggcatgac atagtgcgtg gtagggaaga tgattacgcg
     2941 tctgaaccgt tcgagggcct tgcctgtgag agggtcgatg ccggatatgg atactaccct
     3001 gtcctcatcg tggatgatcc tgaacgccct gtcctcctcg tacggtggaa aaacgtctat
     3061 cgtgccgccg cggacgcgga acctgccccg gtagaagtcc acgtcgttgc gctcgtactg
     3121 accctgaacg agccgttgaa ggatggcctg ccgttcgatc acctgccctt cctcgatggt
     3181 gaggagcatc ccgtagtacg tctcgggcga gccgaggccg tagatgcagg aaacgctggc
     3241 aacgatgatg atgtcctccc gttcgaagag cgcgttcgtt gccataagtc tcatcctgtc
     3301 tatctcttcg ttgatggagg agtctttctc gataaaggtg tccgtggaag gtatgtaggc
     3361 ctcgggctgg tagtagtcgt aatagctaac gaagaagtgg acctcgttgt cgggaaagag
     3421 cgtcttgaac tcggcgtaga gctgcgccgc cagagtctta ttgtgggaga tgacaagggt
     3481 cggcctgttg accttttcta tgacgttcgc catggtaaag gtcttgcccg agccggttac
     3541 gccgagcagt acctggtggg caaccccttg aaatatattc ccggcgatct tctctatggc
     3601 cgcgggctgg tctccctttg cctcgtaatc actgacaagc ttgaacctct tcataacggt
     3661 atagtgtaat gtccttttga cggcatgtca aaaggacagc ttgaacagct tgaaccgctt
     3721 aaacagctcg aacgtcagaa gcaggaggcg gagaatcatc ttctcctgaa aaaaggattc
     3781 gttcttttgg cgctcaagcg gttcaagctg ttcaagcggc tcaagctgtt ttttttctta
     3841 aagtccgatg gcgttgtgtc cgataagatt ttcagtgcag cacgaggaaa aatgttgtgc
     3901 agaggcgcgt ccagaccgaa aagctcttga catggcgtat gcaaaagttt aaagatttat
     3961 catcaatagt tcaaatcatt aatcttagga ggaaggggtc atgaaaaagg ttttgttgtt
     4021 tgcacttgtc atgcttatca gcattgcatt tgtcggagcc ggttttgccc aggaaaaggc
     4081 gaaggccccg gagaaggcga aaccagccgc tgagaagcct gcggtagaga aacccgcgcc
     4141 ggcaaaagaa gagaaaccgg cagaggcaaa ggagcctgag aaagcggcag aaaaaccgaa
     4201 accgaaaccg atggctggtt ttgtcggaaa ggtcaccaag accgactcgg tgttcaaaac
     4261 gatctgtgtc cagcgcggca aggatgaggt cgcattcgat gtcagcgaag ccaagctgaa
     4321 aggttacaag aacctcgacg agatcaacgt tggcgacaag gttgccgttc agtacaagaa
     4381 gggcgccatg acgctgatca agatagcggg agccaagaag gccaaggccg ccaagaaaga
     4441 cgccgtggcg aaaccgaaga aagagaagaa ggaagccgcg actgacgaac agaagcccgc
     4501 ggaagagaag aagcctgcaa agaaggccaa gaaagcaatg aaggacgaga agaaagagga
     4561 aaagaaggac gagaagaaat aacctcttct tcaagatagc gaccatagag gtcccgcctt
     4621 cggcaacgag ggcgggactt ttgtgtttgg ggagaccggt gatctcggcg acgacgggag
     4681 tcgaggaagg gaagacggag ggagcgcggg cttggatagg acctataggt cctatcctcc
     4741 tataggtcct attattacgg cagcggggta cagaggaaaa gcggatgatg ggtgaggcga
     4801 gagactagat tagggggcag gggaaatacc tggcgttgta tcgacaggat cctgcctgga
     4861 cgataaaggg tgaggtcggt tttaaagaga aggcagcgga ctggtagggc catccggccc
     4921 cgacggggtg gtcgtaaccg cagggatggc ccttgaaagg accatccctg cagagctttt
     4981 ttagagttta cctacaaaaa tgaaggcgat aacgagggcg tagatgacca gtgattcgat
     5041 gagggcgagg ccgatgatca tcggggcgaa gatctttccc gatgcgcccg ggttcctggc
     5101 aatgccttca agggctgccg ccgcagcttt accctggccc aatgcgccgc caagagccgc
     5161 gatgccgata gcagcgccgg caccgatggc caacatggcc ttcacatttg aatcgagggg
     5221 agcctttgcg gctgcttctt cggccgcaag cacaaagccg caggaaagaa cgaggatcaa
     5281 accgctgagc atcaaaatgg aaacaacctt tttcatcact attcactcct ttcttttttg
     5341 tattcttgca tcattatatt aatgttcatg ggaaaccgca cctgcaaaat agaccatgga
     5401 gagcagcgtg aaaacgaatg tctggacgaa ggcaacaaag agaccgagga acatgaagat
     5461 gaccggtatc agaagcggaa ccagggcgaa gaatatggcg gtaacaaggt ggtcaccggt
     5521 tatgttgccg aagagc
//
LOCUS       DAOQTR010000068         5524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16216_length_5524_cov_4.211922, whole genome shotgun sequence.
ACCESSION   DAOQTR010000068 DAOQTR010000000
VERSION     DAOQTR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16216_length_5524_cov_4.211922"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKN85_02605"
     CDS             complement(<1..330)
                     /locus_tag="PKN85_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT43320.1"
                     /translation="MKRQVMKIGRHLAVVIGVLALLVLPGGFLAWADTNGLDQALLEA
                     SAKGDLAVVRSLLEKGADVNARTDMGETALHLASSEEVVRVLIEHGADVNATDAEYGM
                     TPLFNQRI"
     gene            471..782
                     /locus_tag="PKN85_02610"
     CDS             471..782
                     /locus_tag="PKN85_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNT43321.1"
                     /translation="MKDIHETLTILKKEFPRVYEAHEALGKEIHEGSGPIPERTRWLI
                     KIAISGAASHRLSLETHIAKGREAGLTDGEIKQALLLLIPTVGFPTFMEAYSVYLADG
                     G"
     gene            840..1301
                     /locus_tag="PKN85_02615"
     CDS             840..1301
                     /locus_tag="PKN85_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43322.1"
                     /translation="MLDRNVCGRIILAIVLIMLPLAAGAGDISGDVEKVLRQILQDRP
                     VPSLDYLQKAEAINTGCAYYRGSYRGIAITVETHPDSDRVASLLLEIPGGDWTREIFP
                     AVRRVIGPERYKRPKQSEYGWEWPDFRAASVHYVKGEKPGEGLTVVSLFYR"
     gene            1413..1622
                     /locus_tag="PKN85_02620"
     CDS             1413..1622
                     /locus_tag="PKN85_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43323.1"
                     /translation="MLEMKSDTITVQKGKEKWEIARDPSTKVTGDVKVGSKVTIEYTI
                     KAATIEVKDAGKTAKPKKGESAPGK"
     gene            complement(1748..2572)
                     /locus_tag="PKN85_02625"
     CDS             complement(1748..2572)
                     /locus_tag="PKN85_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNT43324.1"
                     /translation="MLDALTSFDRFLAVYLIPVGWKVLGAIVLWIVGSWLIKIIGNLS
                     GRGMRAHKLDPTLVKYFDGTIRIILKIVLIIAILSIFGIQTTSFVALIAAAGIAIGAA
                     WGGLLANFAAGAFLIILRPFHVGDMISAAGVTGDVREIGLFATTIDTLDNLCVVVGNN
                     KVLSDNIINYTRNPFRRVDLKAQLAHGVDPREAADRLMERIKNIPNVVDTPEPCVEIL
                     EFNLAGPVLAVRPFCNNKDYWQVFFDTNKAIQEVCATAGYPAPSTNQVIYHKPLAT"
     gene            2830..3705
                     /locus_tag="PKN85_02630"
     CDS             2830..3705
                     /locus_tag="PKN85_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNT43325.1"
                     /translation="MARRCGKRLGSVARKVRGALSLLWQALLQFYDDNGFFLSSGITF
                     NIFITLVPFIILLLAVVGSYLHNDQEVLGHMRAYLRSAAPAMDPGIMNDLMNVIERRQ
                     AIGIAGFAGLVWFSTWLFASLRVALHIVLKVARPRGIVHAFGIDLLMVGAVGVLFLMN
                     MALSSFFAFLQSHQEWIPALAGPAIRLTLRYLLPFFLTFCVFSLIYKVIPNEVVNLRS
                     TLKVSLLTSLFWEAAKHFFAWYVSNIAEYSLVYGSLSTLVVFVLWIYYSSTIFVVGGE
                     LLYLLEKERKAVPVD"
     gene            complement(3845..4429)
                     /locus_tag="PKN85_02635"
     CDS             complement(3845..4429)
                     /locus_tag="PKN85_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43326.1"
                     /translation="MEEREITERICNLFEETVPILNNIYRGFVGQKINLLKESRTKFR
                     ESLKERLPGIEKVVEQKDKTEAEQKFLTALPHLQRVALALDSLLDKMEIKVESNVLFT
                     DKALDEIKQLMVAVGAEFTDVKDFCFTRNPVLKKQIESDLDKVTKMIDEFDHVHQNRL
                     ITGVCMPQASYLYIDMTDSLKRMSRELAAFANTF"
     gene            4575..5018
                     /locus_tag="PKN85_02640"
     CDS             4575..5018
                     /locus_tag="PKN85_02640"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNT43327.1"
                     /translation="MTCAAGTEKKPSPQVVTAAVIERDGRVLIGKRKRGKRFAGNWEF
                     PGGTLEKGETPEECLTRELREELDLEVEVGELFCSATADYAPGWTVRLLAYRAMVISG
                     TFSLNDHEEIRWVRPAELADYNFPEADKPVVEKLVSLGERRDRSE"
     gene            5038..>5524
                     /locus_tag="PKN85_02645"
     CDS             5038..>5524
                     /locus_tag="PKN85_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43328.1"
                     /translation="MRKTAIKILVMGAALALVLFVVFMINQTASLVALGDRIHPLAGD
                     IVLWVLIAFYTLCVIVPVYLFITLPAPLVPPRSEISPEFPRYLQEMAKRLGRNPHIGR
                     TVAASKEDVENALRELERRADDAMKKAAGRIFLATAISQNGKLDGLIVLAAQSRLVLD
                     IA"
BASE COUNT         1112 a         1525 c         1682 g         1205 t
ORIGIN      
        1 gattcgctgg ttgaaaagag gcgtcatccc atactcggcg tccgtcgcgt tcacgtccgc
       61 gccgtgttcg atgaggacgc ggaccacttc ctcggagctt gccaggtgaa gcgccgtctc
      121 acccatgtcc gtgcgcgcgt tcacgtccgc gcccttctcc agaaggctcc tcactaccgc
      181 cagatctccc tttgcggaag cttccagcag ggcttgatcg agaccgttcg tgtccgccca
      241 cgcaaggaaa ccccctggca agaccaggag ggcgaggacg ccgatgacaa cggccaaatg
      301 tctgccgatc ttcataacct gtctcttcat tgtgaagcct ctccagggtt tttgtcaaga
      361 ccggcaggga aggatgcccg gccggacggc ttgcgcttga caaaaggggt cgcgccgtct
      421 aaagaatagt gtatgtcaac cgtttcaagt ccgtacagga ggtgaccgcc atgaaagata
      481 tccacgagac cctgaccatc ctgaagaaag agtttccccg ggtatacgag gcccacgagg
      541 cactggggaa agagatccac gaggggtccg gccccatacc ggagaggaca agatggctca
      601 tcaagatcgc catctccggg gccgccagcc accgtctatc gctggagacg cacatcgcaa
      661 agggcaggga agccggtctt accgacgggg agatcaagca ggcccttctc cttcttatac
      721 cgacggtggg cttccccacc tttatggagg cctactccgt gtatctcgcg gatggcgggt
      781 aacggggggc atcccatgag gctgtagagg cggcagatca caatacctgg agttgcgcaa
      841 tgctggatcg aaatgtctgt ggacgcataa ttcttgccat cgttctgata atgttgcctc
      901 tcgcggccgg tgccggggac atctccggcg acgtggagaa ggtgcttcgt cagatccttc
      961 aggacaggcc tgtaccctca ctcgactacc tgcagaaggc cgaggccatc aataccggct
     1021 gtgcttatta ccgggggagc taccggggca tcgcgatcac cgtggagacg catccggaca
     1081 gtgaccgcgt ggcatctctc ttgcttgaga tcccgggtgg ggactggacc agggagatat
     1141 tccccgccgt gaggcgcgtc attggccccg agcgctacaa gaggccgaaa cagtccgaat
     1201 acggctggga gtggcccgac ttccgtgctg cttcggttca ctacgtcaag ggtgagaaac
     1261 ccggagaagg gctcacggtc gtttctcttt tctacaggta gtgcggcctc ggacaccacc
     1321 tgatgtttgc ctatcttaaa tacttcgttc cctcaagcgc ttcccggaat tgggcctgtg
     1381 aaggtcccaa gacgtaccag gtgaccgggc cggtgttgga gatgaagtcc gacaccatca
     1441 cggtccagaa gggtaaggaa aagtgggaga tcgccaggga tccatcaacg aaggtgacgg
     1501 gcgacgtgaa ggtgggatcg aaggtcacca tcgagtacac gatcaaggcg gcgaccatcg
     1561 aggtcaagga cgcgggcaag acggcaaaac cgaaaaaggg cgaatccgct ccaggaaaat
     1621 agtcgcggtt tcctcgatca ggagatcgat atgcctgaaa tagagattgt caggctgaag
     1681 gggtctggct taagcggggc gccttgagcc ccgcttaagc ttcttcccgt cggcggcgcg
     1741 ggggtgctta tgtcgccagg ggtttgtggt agatgacctg gtttgtcgat ggtgccggat
     1801 agcccgcggt tgcgcagacc tcctgtatgg ctttgttggt gtcgaagaag acctgccagt
     1861 agtccttgtt gttgcagaag ggcctgacgg cgaggacggg cccggccagg ttgaactcga
     1921 gtatctcgac gcagggctcc ggggtatcaa cgacgttggg aatgttcttt atcctctcca
     1981 tcaggcggtc cgccgcttcc cgggggtcga caccgtgggc gagctgcgcc ttgaggtcca
     2041 cgcgccggaa ggggttgcgg gtgtagttga tgatgttgtc ggagaggacc ttgttgttgc
     2101 ccacaacgac gcacaggttg tccagggtat ctattgtagt cgcgaataga ccgatctccc
     2161 gcacgtctcc cgtgaccccg gcggcggata tcatgtctcc cacgtgaaag ggtctgagga
     2221 tgattaggaa ggctccagcc gcgaagtttg ccaggagacc gccccaggcg gcgcctatgg
     2281 ctatgccggc ggcggcgatg agggccacga aggacgtggt ctggatcccg aagatggaga
     2341 ggatggctat gatgaggact atcttcagta tgatgcggat cgtcccgtcg aaatacttca
     2401 cgagggtggg gtccaacttg tgggcccgca tgccccggcc ggacaggttg cctatgatct
     2461 tgatgagcca gcttcccacg atccagagca ctatcgcgcc gaggaccttc caaccgacgg
     2521 gaatgaggta aacagccaga aaacggtcaa aactggtcaa tgcgtccagc ataacgacct
     2581 cctgtcatgt gtgtttgtct ttcatcgacc gttgtgttcg atcttatttc agtatatcac
     2641 cccggacgtc aacggaggag gtcgcagtat cgccccaccg cctcggggca ctacagacaa
     2701 ggttgcgcgg gtttcgcggt tgtcacgttc gctgaaaatg gtttgattgt tggtccaaga
     2761 tatgaaaaag ttaatgagga aaaccgtgcg gcccgtgttg ttggcccggc ctagggagaa
     2821 aggaagacaa tggcgcgaag gtgtgggaag aggctcggat ctgtggcgag aaaggtacgg
     2881 ggagccctga gccttctgtg gcaggcactg ctgcagttct atgacgacaa tggttttttc
     2941 ctgtcgtcgg gcatcacgtt caacatcttc ataaccctgg tccccttcat cattcttctt
     3001 ctggccgttg tggggagcta ccttcacaat gaccaggagg tgctgggcca catgcgcgcc
     3061 tacctgagga gcgcggcgcc cgccatggac cccgggatca tgaacgatct catgaacgtg
     3121 atcgagaggc gccaggccat aggcatcgcc ggattcgccg gactggtgtg gttctcgacg
     3181 tggctcttcg cgtccctgag ggtggccctg cacatcgtgc tgaaggtggc aaggccccgc
     3241 ggcatcgtgc acgccttcgg gatcgacctc cttatggtcg gcgcggtggg cgtgctgttc
     3301 ctcatgaaca tggcactgag ttccttcttt gcttttctcc agagccatca ggaatggatc
     3361 ccggcactcg cgggaccggc tatccggctg acgttgagat acttgctgcc tttcttcctt
     3421 accttctgcg ttttctctct gatctacaag gtcattccca atgaagtggt gaatctccgg
     3481 tcgaccctga aggtctctct cctgacgagc cttttctggg aggcggcaaa gcatttcttc
     3541 gcctggtatg tcagcaacat agccgagtat tccctggtct acggctcgct gagcaccctc
     3601 gtcgtttttg tcctgtggat ctactattcc tcgacgatct ttgtcgttgg aggggagttg
     3661 ctctatctgc tggaaaagga gcgaaaggcc gtgccggtgg actaaagaag gtcgctcatg
     3721 gcccccggtt ctgaaacaga aaggagcgag gtccgccgcg gggatcaggg tccgggacga
     3781 tatcccgaaa ccccatcccg gctgccgggt tgcccccgga atgtttgaat gtgcgaacca
     3841 ggtttcagaa tgtgttggcg aaggccgcca gttcccgcga catcctcttc aaggaatccg
     3901 tcatatcgat gtacagatac gaggcctggg gcatacacac gcccgttatc aggcggttct
     3961 gatggacgtg gtcgaattcg tctatcatct tcgtgacctt gtccaggtcg ctttctatct
     4021 gcttcttcag cacaggattc ctggtgaagc aaaagtcctt cacgtcggtg aactccgcgc
     4081 cgacggccac catgagctgt tttatctcgt caagggcctt gtcggtaaag agcacgttgg
     4141 attcgacctt tatctccatc ttgtcaagaa ggctgtcgag cgccagggcg acgcgctgga
     4201 ggtggggaag tgccgtcagg aatttctgtt ccgcctctgt cttgtccttt tgttccacga
     4261 ccttctctat cccgggaagg cgttctttca gggattccct gaacttcgtc ctgctctcct
     4321 tgaggaggtt gatcttctgg cccacaaaac ccctgtatat gttgttcagg atcgggacgg
     4381 tctcttcgaa caggttgcat atacgttcag tgatctctcg ctcttccatg tactctcctc
     4441 ctcggttatt tgattgtctt gcatgttata tagcatagga aagctctctt gtcagcgaga
     4501 taccgcacat gggagtaatt gagcggcagg acccgcggct tgtgttcggt cccttctcgt
     4561 gatatggttg cctcatgacg tgcgcggcgg ggacggagaa gaagccttct ccccaggtgg
     4621 tcaccgcggc ggtgatcgag agggacgggc gtgttctcat cggaaaacgg aagcggggca
     4681 agcgttttgc agggaactgg gagttccccg gcggcaccct cgagaaagga gagacaccag
     4741 aggaatgcct cacgcgtgag cttcgggaag agttggacct cgaggtcgag gtgggagagc
     4801 ttttctgctc cgccacggcc gattacgccc cgggatggac ggtgagactg ctggcataca
     4861 gggcgatggt gatatcaggc acgttcagcc tcaacgacca cgaagagata cgatgggtga
     4921 gaccggcgga gctggccgac tacaatttcc ccgaggcaga caaacccgtg gtggagaaac
     4981 tggtcagtct tggcgaaagg cgagataggt cagaatgacc gaaagacagg ggacgtgatg
     5041 cgcaagacag cgatcaaaat actggtgatg ggcgctgcgc tggccctcgt tctcttcgtc
     5101 gtcttcatga tcaaccagac ggcttctctc gtcgcgctcg gggaccgcat ccatccgctt
     5161 gcgggcgaca tcgtcctgtg ggtcctcata gctttctata ccctctgcgt cattgtgccc
     5221 gtgtacctct tcatcacgtt gccggcgccc cttgtcccgc cccgtagcga gatctcgccc
     5281 gaatttccga ggtatctcca ggagatggca aaaaggctcg gacgaaaccc tcacatcggg
     5341 aggaccgtcg cggcctcaaa agaagacgtg gagaacgccc tgcgggaact ggaaaggagg
     5401 gcggacgacg cgatgaagaa ggcagccgga cgcatattcc tggccacggc gatatcgcag
     5461 aacggcaaac tggacggcct tatcgtgctc gctgcccagt cacggctcgt tctggacatc
     5521 gcgc
//
LOCUS       DAOQTR010000069         5522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16226_length_5522_cov_4.194622, whole genome shotgun sequence.
ACCESSION   DAOQTR010000069 DAOQTR010000000
VERSION     DAOQTR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16226_length_5522_cov_4.194622"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKN85_02650"
     CDS             complement(<1..496)
                     /locus_tag="PKN85_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT43329.1"
                     /translation="MVNKKNLIPLCLIPLCLCLLLSCGRKKDPARYDDPGKPAFGDMI
                     ITGSIGEPSNLIPILSVDSASHDVASYVYNGLVKYDKDLIIVGDLAESWEISKDNLSI
                     TFRLRKGVRWHDGRPFTAHDVMYTYRVTIDPKTPTAYSGDFKLVRKAEVLDDHTFRVT
                     YDKPF"
     gene            complement(630..1367)
                     /locus_tag="PKN85_02655"
     CDS             complement(630..1367)
                     /locus_tag="PKN85_02655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNT43330.1"
                     /translation="MNNSEKLEKVEKIVASGLDRQGTAYVLQLTGIIGHPVENLSELR
                     LYSDFLPRVDDFGCTESQRFLHFLWDALDKSPICLNAGFSIPFRRILAKKLFGKCGKN
                     LIAEENVRFNFGQGIEVGDDVFLNRGVYLDSKGGIAIGNSSGLAENVQVFTHSHSESD
                     HTRRTYRKVTIGNYVVVYIASIILSGVSIGDEAIVAAGSVVTGDVETSMMVGGRPAAP
                     IRQRRREGCHGRDLNHLWLKDGLFQDE"
     gene            1527..2471
                     /locus_tag="PKN85_02660"
     CDS             1527..2471
                     /locus_tag="PKN85_02660"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNT43331.1"
                     /translation="MYINAISHYLPSTIIGNGHYTRLNGLSDEWIFRRSGIRQRTRAA
                     DGENTHTMAIDAITPLVGNLPYRISDVDLIVGATYTPYDTVGTLAHAIQGHFNIPRAA
                     AVTITSACSSFVNAVEIVEGYFATGKATKALVIASEHNLAYTDDTDSVSGHLWGDGAG
                     CIFVSAGRYSPDDIEIVDVHTSGLGHIGKGVAGVYLRPSNGGLTMPHGRDIFVHACNF
                     MATEAQHMVERNRYMIDDVDYLIPHQANARIIDHVAKVLGLDNSKVISNIEDTGNTGS
                     ASTVICLSQNWERFMKDDLILVTVFGGGYSSGAMLLRK"
     gene            2762..3322
                     /locus_tag="PKN85_02665"
     CDS             2762..3322
                     /locus_tag="PKN85_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT43332.1"
                     /translation="MELLKAIQERRSVRKYRKDPIPEEILTEILEAARRSPSWANTQV
                     WRFIVVKDLGIKEGLLDCLGPTNPARSAIVEAPVVICVAAKRGVSGCHKGTPTTDKGD
                     WFMFDAGIAMEHIVLAAWNFGLGTVHVGSFDAKKAETVLKIPEGYSITEMAPLGYFDE
                     VPRETPRKPLAELVFLNAFGEPYMKS"
     gene            complement(3639..4019)
                     /locus_tag="PKN85_02670"
     CDS             complement(3639..4019)
                     /locus_tag="PKN85_02670"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNT43333.1"
                     /translation="MPVKLTEAQFDRVVARAIERIPLEIRRYLDNMVIAVKKRPSAGM
                     IKEMGMHPDDYPLGLFQGVPLIERSATTPPLYPDMIYLFQEPLEEMCDTREELEEEIE
                     VTVVHEVAHYIGMTEERLAELGYG"
     gene            complement(4056..4631)
                     /locus_tag="PKN85_02675"
     CDS             complement(4056..4631)
                     /locus_tag="PKN85_02675"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNT43334.1"
                     /translation="MSPSHRVCVPPAGKGPPLAGAVLLLLYPGEDDELFIPLTVRTQN
                     VAVHKGQVSFPGGAHEPQDDSLSHTALREACEELGVCGDDVRIMGALTPLYIEPSNFC
                     VHPFVAWQSYRPSFVPQAIEVSEVLEVPVSHFLDERNVAVEEWLIEGAMRRIPYFGVC
                     GHKVWGATAIILSEFVAVLESLRASGKRDLE"
     gene            complement(4917..5210)
                     /locus_tag="PKN85_02680"
     CDS             complement(4917..5210)
                     /locus_tag="PKN85_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43335.1"
                     /translation="MAAVKPKGKSKVTRKKTSTARGSAVDRSFMTALQALEEMARGQI
                     TSAKDPFKTGIRIVGEIKKVILRLENEIEDDSRSMTEAYDSGPGVVGMGIGVL"
BASE COUNT         1225 a         1667 c         1499 g         1131 t
ORIGIN      
        1 caaagggttt gtcgtaggtc accctgaagg tgtggtcgtc gagcacctcc gccttcctga
       61 cgagcttgaa atcgcccgaa taggccgtgg gagtcttcgg atcgatggtc acccggtacg
      121 tgtacatcac gtcgtgcgcc gtaaaaggcc tgccgtcgtg ccacctgaca ccctttcgga
      181 gccggaaggt tatggacagg ttgtccttcg atatctccca ggactctgcg aggtcaccga
      241 cgatgatgag gtccttgtcg tacttcacga gaccgttgta aacgtaagaa gcgacgtcat
      301 gggacgcgga atcaaccgac aggatgggga tgaggttcga cggttcccct atcgagcccg
      361 ttattatcat gtccccgaag gcgggcttcc ccgggtcatc gtaccgtgcc gggtctttct
      421 tcctgccaca ggagagaagg aggcagaggc acagcgggat gaggcaaaga gggatcaggt
      481 tcttcttgtt taccatgggc gcaattgaag ctaattctag cagagaaccc ctgccaaagt
      541 aaagaaagcc gcgggctgca accctcgcgc tgtcttccgg tcccgaacac cgtgcgctct
      601 tttcccgatg gacctacccc gtatcgccgc tattcgtcct ggaagagacc gtccttgagc
      661 cacaggtggt tgaggtccct gccgtgacaa ccttctctgc gacgttgccg gataggggcc
      721 gcgggcctgc ctcctaccat catggatgtc tccacgtccc cggtgaccac agaccctgcc
      781 gccactatcg cctcatcacc tattgaaacc cccgacagga ttatggatgc gatgtatacg
      841 actacgtaat taccgatagt aacctttcta taggtacggc gggtgtggtc cgattcgctg
      901 tgagaatggg tgaagacctg cacgttctcg gcaaggcccg agctgttacc gatggcgatg
      961 ccgcccttcg aatcgaggta caccccgcgg ttcaggaaga catcatcccc cacctcgatg
     1021 ccctggccaa agttgaagcg cacgttctct tcggcgatga ggttcttccc gcacttcccg
     1081 aaaagcttct ttgccaggat gcgacgaaag ggaatggaaa acccggcatt cagacagatg
     1141 gggctcttgt caagcgcatc ccacaggaaa tggaggaacc gctgactctc ggtgcaaccg
     1201 aagtcgtcga ccctggggag aaaatcgctg taaagtctta gttcgctgag gttctccacc
     1261 gggtggccga tgataccggt cagttgcagg acatatgcgg taccctgtcg gtcaagtccc
     1321 gacgcgacga tcttctccac cttttcaagt ttttccgaat tgttcatgga tcacccggtg
     1381 tctgaattag ttgaaaactt tgagtgaatc ctatataaca tcacggggcc tgtcaacggc
     1441 aaagtgatat ctatgccccc tccgaggacc tgacagacaa atctgccgtc aatgcgctgc
     1501 gacaagagga ctggagcact gtttatatgt acataaatgc aatctcacac taccttccga
     1561 gcacgatcat cggcaacggg cactatacgc ggctgaacgg gctttccgac gaatggatat
     1621 tcagaaggtc gggcatacgg cagaggacaa gggccgcgga cggcgagaac acccacacca
     1681 tggccatcga tgcgataaca ccgctcgtcg gcaaccttcc ctaccggatc tccgatgtcg
     1741 acctcatcgt cggagcaaca tacacgccgt acgatacggt aggcaccctc gcccatgcca
     1801 tacagggcca cttcaacata ccccgggccg cagccgtgac gatcacctcg gcgtgctcat
     1861 ccttcgtgaa cgccgtcgag atcgtcgagg ggtattttgc gacgggaaag gcaacgaagg
     1921 ccctcgtcat cgcttcggag cacaatctcg cctacaccga cgacaccgac agcgtatcgg
     1981 gtcatctgtg gggagacggc gccggctgca tcttcgtctc cgccggaaga tactctcctg
     2041 atgacatcga gatcgtcgac gtccacacct cgggcctcgg ccatatcggg aaaggcgtgg
     2101 cgggagtgta cctcagaccc tcgaacgggg ggctcaccat gccccacggg cgggacattt
     2161 tcgtgcacgc ctgcaatttc atggcgacgg aggcacagca catggtggaa aggaacaggt
     2221 acatgatcga cgatgtcgat tacctcatcc cccaccaggc gaatgcccgg atcatcgacc
     2281 acgtggcaaa ggtgctcggc ctcgacaatt cgaaagtgat atccaacata gaggacacgg
     2341 ggaataccgg ctcagcaagc accgtcatct gcctttcgca gaactgggaa aggttcatga
     2401 aagacgacct tatcctcgtg accgtattcg gcggaggata ttccagcggc gcgatgttgc
     2461 tcaggaaatg aagaccgttc caggttccag gttccagatc aaagacagga cggggcagag
     2521 acgatctcag gcctcaggtt ccggggaagg aacgaagcca aagacgcgcg gtgtcccgcc
     2581 aggttctgta aaggggtcga aagaagattg gagcctttac agaacctttg cgctcccctt
     2641 gcgtgaaaaa tgttgcccgc cactccacca ttcggtcttg tcctggactt gaaacatgga
     2701 actcgagacc tgggaacgtc ttttgttgac acccgactgc cccccgttgc ataatggaag
     2761 tatggaacta ttgaaagcga tccaggaaag gcggagtgtc aggaaataca ggaaggaccc
     2821 gattcccgag gagatactca ctgagatact cgaggcggca cggcgttcgc cttcgtgggc
     2881 gaacacccag gtgtggcgct ttatcgtcgt caaggacctg gggatcaagg aaggtctcct
     2941 tgactgtctc ggccccacca accccgcacg gtcggcaatc gtcgaggcgc ccgtcgtcat
     3001 ctgcgtcgcc gcaaagagag gggtttccgg ctgtcacaaa ggaacaccga caacggataa
     3061 gggtgactgg ttcatgttcg acgccggcat cgccatggaa cacattgttc tcgcggcatg
     3121 gaacttcggc ctcggcaccg tccacgtggg aagcttcgac gcgaagaagg cggagaccgt
     3181 actgaagatc cccgaaggat actccatcac cgagatggcc ccgctcggct atttcgatga
     3241 ggtcccccgc gaaacccccc gcaaacccct cgcggaactg gtcttcctca acgccttcgg
     3301 ggaaccctac atgaaaagct aagccgaagg gcggcttgag tcgcttgagt cgcttgaggg
     3361 tcagaaacca ggaatactcc tacataacac atattccgcg gcctgattcg gagcacaagg
     3421 attctccgac cgcttttcga acccttaaca ggctgttgat aaacccattt tctacgtcat
     3481 tccggcttta ggcccgaatc cagaggtttc tctaacccct cgtttttcct ggattccggc
     3541 cttcgccgga atgacgaaag agcacgaatc gtcttcagaa atcactttgt caacagcctg
     3601 ttaagcggtt caaaccattc aagcaactca aaccgccttt acccgtatcc cagttccgcg
     3661 agtctttcct cggtcatgcc tatgtagtgg gccacctcgt ggacaaccgt gacttctatc
     3721 tcttcttcca gttcttcccg ggtgtcacac atctcttcca ggggttcctg gaagagatag
     3781 atcatgtcgg gatagagcgg aggtgtcgtc gcggaacgct cgataagggg aacgccctgg
     3841 aaaaggccca ggggatagtc atcgggatgc attcccatct cctttatcat gcccgccgaa
     3901 gggcgtttct tcaccgctat gaccatattg tccaggtacc ggcggatctc cagggggatc
     3961 ctttctatcg cccgggcaac cacgcgatcg aattgagcct cagtgagctt gacgggcacc
     4021 ggtggcctcc ttgaaaaaga attgaacgac ggggatcact caaggtcgcg cttccccgag
     4081 gcgcgaaggc tctcgaggac cgcgacgaac tcgctgagga ttatcgccgt cgctccccat
     4141 accttgtgcc cgcagacacc gaaataggga atgcgcctca tagccccttc tatcagccat
     4201 tcctcgacgg caacgttcct ctcgtcaaga aagtgggaga ccggcacctc aaggacctcg
     4261 gagacctcga tcgcctgggg aacaaaggaa ggacggtagg actgccaggc aacgaagggg
     4321 tgaacacaga aattgctcgg ttctatgtac aggggtgtca acgcgcccat gatcctcacg
     4381 tcatcgccgc agacccccag ttcttcgcaa gcttcacgaa gcgcggtatg ggaaagcgaa
     4441 tcatcctgcg gctcatgggc ccccccggga aaactgacct gtcccttgtg aaccgccaca
     4501 ttttgcgtgc ggaccgtgag agggatgaag agttcgtcgt cttcccccgg gtacagaagg
     4561 aggaggacgg ctcccgcgag aggcggcccc ttcccggcag gcggtacgca cacccggtgt
     4621 gatggcgaca tgcgccgttg ggcgtcatgg cccggcagtg ccatcctcaa cgcccgttct
     4681 atctcgtccg gtgtgagtct atctccgata ctgtcctcgc aaaaactacc gccgtctgcg
     4741 gacaacccgc ccgaatacag acccccgcca ggttggtccg cgcggcacga aagaccattc
     4801 accgtcgtgg cccttttcaa gcccggcagg aaccaggttt gccgcgtccg gggacccggg
     4861 gaagcggcga tcacgtgtga tctgacccgc tggcgctcct aagcagacgg gtcgctctac
     4921 aaaaccccga tgcccatccc cacaacgccg ggtccggaat catacgcctc ggtcattgac
     4981 ctcgagtcat cctcgatctc gttctcaagg cgcagaatga ccttctttat ctctccgacg
     5041 atcctgatcc ccgtcttgaa aggatccttc gccgatgtga tctgtcctct cgccatctct
     5101 tccaacgcct gcaacgctgt catgaaactc ctgtccacgg ctgaacccct ggccgtcgac
     5161 gtcttcttcc gggtgacctt gcttttcccc ttcggtttca ctgccgccat acaacacctc
     5221 ctgggtaagt tagacgtaca atcaccgaat cagctcagag catgcgccac ctcagcgcga
     5281 cccctcgcag ccccaaggaa cgtccatatt tcaattatat tgctaatacc cggttcgtca
     5341 agcaaagaac cgtcgcgggt cacaggtcgc caaggaaagg cgatggaaga agtgcattgg
     5401 atccggcgga tctttttttg cctttagacc tgtgacctgc gacgggcttt cgccagaaaa
     5461 gccctcgggc cgggaaccac gctcatcccg cgacgagcac ctgccagatg aaggtaccta
     5521 tc
//
LOCUS       DAOQTR010000070         5521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16229_length_5521_cov_3.079583, whole genome shotgun sequence.
ACCESSION   DAOQTR010000070 DAOQTR010000000
VERSION     DAOQTR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16229_length_5521_cov_3.079583"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKN85_02685"
     CDS             <1..933
                     /locus_tag="PKN85_02685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT43336.1"
                     /translation="TKENPVGYRIQQYFPYLEERGFKISLLTTKADLGTVLKTASRSD
                     VVVVQRILLSPMKLSLLKMFAKKMVYDFDDAVMYGSKGESATRRSRFAAIVKASKAVL
                     CGNSFLMEEAGRHRNSGIHYMPTTVDIDEYPVKTHEERKTFVVGWIGTSSTLRYLDGI
                     RDLILSITDREGVEFRVIADKPFGVEKRGITFQRWNKEREKSMLLDFDMGIMPAEDDT
                     WSRGKCGLKLIQYMAMGLPAVTNPVGVSKEIVADGVNGFLRTDPGGWDEAIRTLSSDV
                     ALRRRIGAAARQTAMERYSLQTWGKRFAEIVDSL"
     gene            930..2351
                     /locus_tag="PKN85_02690"
     CDS             930..2351
                     /locus_tag="PKN85_02690"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNT43337.1"
                     /translation="MKDPDGLITANVNGIEWHLEDRGLVEVLQPFSHEGGERRLYGTY
                     PFRAGRVFVKSFLEKGLLATIRNRVHPRGRIEYLMSRKLASLGISTPVSYGYGRGPGT
                     SCVVHEYIDGESFIDVFRGSGEREKLIAALAELLRDLEERHILHNDLHLNNVLVSKGR
                     LYLIDLHKMKIKGSFAAADEISNLSHALAMAYWTMTPDEKETFFRCYGREGLRGEVEG
                     TLRAMHMRWISRKQRRAFENTSKTRWEGRCLHVRGREDRGQGAYVETVKKDTKVAVER
                     YTDHMRKIYGGRRRLKRAWKAHIALVYLNLPLIPDTFHLVMPALFRPGYIAMEDLGVR
                     GEELDRHVDRVYDGASGTQRKVLADSLALFLKGALRTGVVHRDLKACNVFRLNDGTFR
                     FLDVEDIGFREMDGDVLQRMLLQLNTTLPRRIATRDRLRFLARLTSSTGLDRRQLLEK
                     VARDSTGREIVYQGTDGLKIESW"
     gene            2409..4658
                     /gene="bamA"
                     /locus_tag="PKN85_02695"
     CDS             2409..4658
                     /gene="bamA"
                     /locus_tag="PKN85_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNT43338.1"
                     /translation="MKKLKCLILLFLILCLPVLIQAQEAERVAKIEITGNERVDTAVI
                     SNNIKTKEGDAYNLDKLREDMKNIYKTGFFSDVQIDVKDTDKGKSVTFVVIERPPVRD
                     ILMTGNKKIKTDDLKDKLKIKTGTVLNIEKVKESMDEIRKLYASKGYYAAKITYEIDY
                     GEKYEATVNFVIDEPQLSYVRKIAFTGNKSFKDSKLQGYMQTREKGWFSWFTGSGILD
                     EDVLQEDRRNIEAFYHDNGYTKVHVGAPDIQLSKDGKSISITIPVEEGAKHTVGTIDF
                     AGDIIFPREELLEKIKTKQGKLFRTTVFYEDVLTVTDLYQDKGFAFADVAPLTTIDDA
                     NHVVNVTFQVTKNQEVYFNRINIVGNTRTRDKVVRRELKFAEGDRFSITAIKDSKKKL
                     KNTTFFKDTDFKIVKTDDPRKVNIDLGIEEKPTGTFAIGAGYSTAEKVIVSGSVSEEN
                     FLGTGRKVVLEAALSSYTNEFRLSYLEPYIFDKNLSVGTSLYNFKRYMDTYDYKRMGG
                     NLSLIKPLTDDVKGTVMYRLENIDVTNVTNDASSYIKQQSGSSLTSSMTFSLNKNTID
                     DVMNPTKGINSGISTEIAGGPLGADNKFWSITGFYGRYFPITFWDSSFFVKGTAGSIR
                     GYGGDEVPITEKYYVGGLNSIRGFRYGEAGPMDENDEPIGAKNQMYFNFEWIFPIYKP
                     AGVKGVLFTDTGAGFDNFDSLRMRSSAGFGIRWFSPMGPIRLEMGFNLNPKENERKSL
                     FDFAIGTQY"
     gene            4671..5204
                     /locus_tag="PKN85_02700"
     CDS             4671..5204
                     /locus_tag="PKN85_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNT43339.1"
                     /translation="MKRFITVFFVAAVLLTPLAAMSQSVSVAYIDLQKVMLESDQGKQ
                     IRAILTQDAERLKKNLDGKQAELQRLKDAFEKQGATITPEARADKEKQYQAKLKDYQR
                     LYNDYQSELQQKDMEYTQKVLKEVEEVVRALGEKEKYSLILEKSQAGILYATPAIDIT
                     GKVITLFNQAAKNKPLK"
     gene            5204..>5521
                     /locus_tag="PKN85_02705"
     CDS             5204..>5521
                     /locus_tag="PKN85_02705"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HNT43340.1"
                     /translation="MIRLRDIAAAVKGDLIGDGDIPIEGIAGIQSAREGQITFLSHAS
                     YVKHVAGCRASALIIGQKTPRDDVRGMNIVVVENPDLAYIEVVGMFRKEPSPPAGIDP
                     RAAV"
BASE COUNT         1388 a         1562 c         1582 g          989 t
ORIGIN      
        1 accaaggaaa accctgtggg atacaggatc cagcaatact tcccgtatct cgaggagagg
       61 ggtttcaaga tctccctgct gacgacgaag gcggacctcg gcaccgttct caagacggcc
      121 tcgcgatccg acgttgtcgt cgtacagagg atccttctga gccccatgaa gctctccctc
      181 ctcaagatgt tcgcgaagaa gatggtctac gatttcgatg acgccgtcat gtacggcagc
      241 aagggtgaga gcgcaacgag aaggagcagg ttcgccgcca tcgtcaaggc ctcgaaggcg
      301 gtcctctgcg ggaactcctt tctcatggag gaggccggca gacacaggaa ttccggcatc
      361 cactacatgc ccaccaccgt ggacatcgac gaataccccg tgaaaacgca tgaagagcgc
      421 aagaccttcg tggtcggatg gatagggacg agttccaccc tgcggtacct cgacggcatc
      481 agggacctca ttctttccat caccgacagg gagggggtcg agttcagggt catcgcggac
      541 aaacccttcg gtgtcgagaa gcgggggata acctttcaaa gatggaacaa ggagcgggag
      601 aagtcgatgc tcctcgattt cgacatgggc atcatgcccg ccgaggacga cacgtggtcc
      661 aggggaaagt gcggtctcaa actgatccag tacatggcaa tggggctccc tgcggtcacg
      721 aaccccgtcg gcgtctcgaa ggagatcgtc gcggacggcg tgaacgggtt tttgaggacc
      781 gaccccgggg gatgggacga ggccataaga acgctcagct ccgacgtggc ccttcgccgg
      841 cgcatcggcg cggcggcacg gcagacagcg atggaaaggt attcactgca aacctggggc
      901 aagaggttcg ccgagatcgt ggattctcta tgaaagaccc cgatggattg ataaccgcca
      961 acgtgaacgg catcgagtgg cacctcgagg acagggggct cgtcgaagtc ctgcagccct
     1021 tctcccacga gggaggagaa cgaaggctct acggcaccta tcccttcagg gccggcaggg
     1081 tgttcgtaaa gtcctttctg gaaaaaggcc tcctcgccac gattcggaac agggtgcatc
     1141 cccggggacg catcgagtat ctcatgagcc ggaaacttgc ctctctcggg atatcgacgc
     1201 ccgtgtccta cggttatgga cggggaccgg ggacctcctg cgtcgtccac gaatacatcg
     1261 atggggaaag cttcatagac gtcttccgcg ggtccggcga acgggaaaag ctcatcgcgg
     1321 ccctcgcgga gctgctccgc gatctggagg aacgccatat ccttcacaat gacctccacc
     1381 tgaacaacgt gcttgtttcg aaaggccggc tctaccttat cgacctccac aagatgaaga
     1441 taaagggctc tttcgccgcc gccgacgaga tctcgaacct ctcccatgcc ctggccatgg
     1501 cctactggac catgaccccc gatgagaagg aaacgttctt ccggtgctac ggcagggaag
     1561 ggttacgggg tgaggtcgaa gggaccttaa gggcaatgca catgagatgg atatcgagaa
     1621 agcagcggcg ggccttcgag aacacgtcga agacacggtg ggagggcaga tgtttgcacg
     1681 tccgtggcag agaggaccgc ggtcaggggg cctacgtcga gaccgtcaag aaggacacga
     1741 aggtcgccgt cgagaggtac accgaccaca tgagaaagat atacggcggc aggaggaggc
     1801 tgaaaagggc atggaaggct cacattgccc tcgtctacct caaccttccc ctgatccccg
     1861 acacgtttca tctcgtcatg cccgcccttt tccggcccgg gtacattgcc atggaggatc
     1921 ttggggtaag gggcgaggaa ctggaccgtc acgtggacag ggtatacgac ggggcgtccg
     1981 gtacacaaag gaaggtcctg gcggactccc ttgccctctt cctcaaggga gcgctgagaa
     2041 caggggtcgt ccaccgcgat ctgaaggcct gcaacgtctt cagactgaac gatggaacat
     2101 tccgtttcct cgatgtcgaa gatatcggtt tccgggagat ggacggggac gttctccaaa
     2161 ggatgctcct tcagctcaac accacccttc ccagaaggat cgcgacgcgg gacaggctga
     2221 gattcctcgc caggctcacc tcttcaacag gtctcgacag gaggcagttg ctggaaaaag
     2281 tggcccgcga ctccaccgga agggagatcg tctaccaggg aacggatggg ctcaagatcg
     2341 aatcgtggtg acggcctttt ttttaccttt acaaagagga tgtttttttg gtaattttgc
     2401 aaaacattat gaaaaaattg aagtgtctta tcctcctctt cctgatcctg tgtctgcctg
     2461 tcctgatcca agcccaagaa gccgagaggg ttgccaagat cgagatcacc ggaaatgagc
     2521 gcgtcgacac cgctgtcata tcgaacaaca tcaagacgaa ggaaggtgac gcgtacaacc
     2581 tcgacaagct ccgcgaggac atgaagaaca tctataagac gggttttttc agcgacgtcc
     2641 agatagacgt gaaggacacc gacaagggca agtccgtcac tttcgtcgtg atcgagcggc
     2701 ctcccgtgag ggacatcctg atgacgggga acaagaagat caagaccgat gacctcaagg
     2761 acaagctgaa gatcaagacc gggaccgtcc tcaacatcga aaaggtcaaa gagagcatgg
     2821 acgagatccg caagctctat gccagcaagg gctactacgc cgcaaagatc acctacgaga
     2881 tagactacgg ggagaaatac gaggccaccg taaacttcgt aatagacgaa ccgcagctgt
     2941 cctacgtgag gaagatagcc ttcacgggca acaagagctt caaggacagc aagctgcagg
     3001 gctacatgca gaccagggaa aaaggctggt tctcgtggtt cacgggatcg ggcatactcg
     3061 acgaggatgt ccttcaggaa gaccgcagga acatcgaggc cttctatcat gacaacggct
     3121 acaccaaggt ccacgtgggc gcccccgaca ttcaactctc caaggacggc aagagcatct
     3181 ccatcacgat acccgtcgag gagggcgcga aacacaccgt cgggaccatc gatttcgcgg
     3241 gagacatcat cttcccgcgg gaagagctcc tcgaaaagat caagaccaag cagggcaagc
     3301 tcttcaggac caccgttttc tatgaagacg tcctcacggt gacggacctc taccaggaca
     3361 aggggttcgc gtttgccgac gtcgcgccgc tgaccaccat cgacgatgcc aaccacgtcg
     3421 tcaacgtcac cttccaggtt acgaagaacc aggaggtgta cttcaacagg ataaatatcg
     3481 tcggcaacac gaggacccgg gacaaggtcg tgcgcaggga gctgaagttc gccgagggcg
     3541 accgcttctc catcaccgcc atcaaggaca gcaagaagaa gctcaagaac accacgttct
     3601 ttaaggacac cgatttcaag atcgtcaaga ccgatgaccc caggaaggtc aacatcgacc
     3661 tcggcatcga ggaaaaaccc acgggcacct tcgctatcgg ggcaggctac agcacggcgg
     3721 agaaggtgat cgtttccggg tccgtctcgg aagagaactt ccttggcacg ggccggaagg
     3781 tggtcctcga ggccgcgctc agcagttaca ccaacgagtt ccggctcagc tacctggaac
     3841 cctacatctt cgacaagaac ctctccgtcg ggaccagtct ctacaatttc aagcggtaca
     3901 tggatacgta cgactacaag cggatgggcg gcaacctgtc cctcatcaag cccctcaccg
     3961 atgacgtcaa gggaacggtc atgtaccgtc tcgagaacat cgacgtcacg aacgttacga
     4021 acgacgccag cagctacatc aaacagcagt ccggttcgag cctgacgagc agtatgacct
     4081 tcagtctcaa caagaacacc atcgatgacg tcatgaaccc cacgaagggc ataaacagcg
     4141 gcatatcaac ggaaatagcc ggcggtcccc tgggagccga caacaagttc tggtccatca
     4201 ccggttttta cggcaggtac ttccccatca ccttctggga cagctccttc ttcgtcaaag
     4261 gcaccgcggg atcgatcagg ggctatggcg gggacgaggt gccgatcacg gagaaatact
     4321 atgtcggcgg tctgaactcc atccgcggtt tcaggtacgg cgaagccggg cccatggacg
     4381 agaacgacga gcccatcggc gcgaaaaacc agatgtactt caacttcgaa tggatattcc
     4441 ccatctacaa gccggcaggg gtgaaggggg tccttttcac cgacaccggc gccgggttcg
     4501 ataacttcga cagcctgcgg atgcggtcat cggccggatt cggcatacgc tggttctctc
     4561 ccatgggtcc tatccgcctg gaaatgggct ttaacctcaa ccccaaagaa aacgaaagga
     4621 agagtctttt cgattttgcc atcggtacac aatattagtc aggaggtttt atgaaacgct
     4681 ttatcacggt cttttttgtt gcagccgtac ttttgacacc acttgcggcg atgagccagt
     4741 cggtcagcgt cgcgtacata gacctgcaga aagtgatgct tgaatcggac cagggcaaac
     4801 agatacgggc cattctcacg caagatgccg agagactgaa aaagaacctc gacggaaagc
     4861 aggccgagct ccagaggctc aaggacgcct tcgagaaaca gggcgccacc atcacgcccg
     4921 aagccagggc cgacaaggaa aaacagtacc aggcaaagct caaggattac cagaggctct
     4981 acaacgacta ccagtccgag ctccagcaga aggacatgga atacacgcag aaggtcctca
     5041 aggaggtcga agaggtggtg cgcgcccttg gtgagaagga gaagtattcc ctcatacttg
     5101 aaaagagcca ggccggcatc ctgtacgcga cccccgccat agacatcacg ggcaaagtga
     5161 tcacactctt caaccaagcg gccaagaata aacctctcaa gtaatgatca gactgaggga
     5221 tatcgctgcc gcggtcaagg gagatctgat tggggacggg gatatcccga tcgagggcat
     5281 tgccggcatt cagagcgccc gggaaggaca gatcaccttc ctctcccacg caagctatgt
     5341 caagcatgtc gccgggtgca gggcctccgc ccttatcata gggcagaaga ctccccgcga
     5401 tgacgtccgg ggaatgaaca tcgtcgttgt ggagaacccc gacctcgcgt atatagaggt
     5461 cgtgggcatg ttccggaagg aaccctcccc gcctgccggg atagacccgc gcgccgcggt
     5521 a
//
LOCUS       DAOQTR010000071         5518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16242_length_5518_cov_3.070108, whole genome shotgun sequence.
ACCESSION   DAOQTR010000071 DAOQTR010000000
VERSION     DAOQTR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16242_length_5518_cov_3.070108"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..625)
                     /locus_tag="PKN85_02710"
     CDS             complement(263..625)
                     /locus_tag="PKN85_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43341.1"
                     /translation="MKKGRTGVRGGNGRGPGLFLISLAIAASFLGFGAGASLSGDSAG
                     LSGDISSYSGQASEAHSSGGSYLQTYRARTYHCETKNIHTGVCSCPAGYSAQLVALAE
                     GPSCDYEPCWITYGYVCE"
     gene            complement(901..>1281)
                     /locus_tag="PKN85_02715"
     CDS             complement(901..>1281)
                     /locus_tag="PKN85_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43342.1"
                     /translation="AGLSEGSYQRPTGDLTAPTSFGLFTQASWWITDSLSLNGMYGYL
                     KFNYSGYGRSFSATARDRINMSQTYAANLLWDANQAIRFGIQWIRNFSGYNGYGTPGT
                     SVGTGNADRTGVADMYRFGAWYFF"
     assembly_gap    1282..1291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1292..2218)
                     /locus_tag="PKN85_02720"
     CDS             complement(<1292..2218)
                     /locus_tag="PKN85_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43343.1"
                     /translation="MKKCLLVLFVVSLILPVLCNAGSVSSRYDVTFGGFVKFDLGFTS
                     QNGNADASTAARSSTSSRKVLADEYGNTFMSGGETRFNFLVKGPDLWGAKTSAFIEGD
                     FRGVTTGNQYGGFNLRHAFMKLKWQSAELIAGQTWQQFGMPYYGAWMGAGDVTEYLRG
                     IRLPQIALRYFFTREFNAMFGLVSATEWSGANNYYANGVRGSNDGFARSSWPGFEGEI
                     AYWTDRCGKIGPNNLKFALGGYYGKDNGTSATYLTAAGNDYRDDTINSWVAAFRYSIP
                     IVPERKGNKAMAVLLNGNFFIGQNIAGNNWLGT"
     gene            2878..3714
                     /locus_tag="PKN85_02725"
     CDS             2878..3714
                     /locus_tag="PKN85_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNT43344.1"
                     /translation="MFANPVPYAGAFAGRWSQIVDLERIYKREGPAVIRRMDGDPVSF
                     VHRWHLDADREIAGFIAAQFAYGRIEVFMGFLEKLFALMDEGPERFVRRGAWEGLKGL
                     YYRFQKEDDLVHLFEVLHRIVGDHGSLGAMFRSFYEGDTRRAVWRIRDAYLAGDDRLT
                     FFFPRPSSTGAQKRWSMFLRWMVRKDEIDIGIWDFIDPADLTIPLDTHIYKVGRCLGW
                     TGRKTPSYRAAREITEALKRYCPSDPLKYDFFLCHGVGIGAGCTGKRSDRCREKCAVH
                     EI"
     gene            3704..4234
                     /locus_tag="PKN85_02730"
     CDS             3704..4234
                     /locus_tag="PKN85_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43345.1"
                     /translation="MRFRIVSLGCPKNLVESEYMVGRLCSAGHTVSDDGDTIIVNTCA
                     FIAEAARESIETILEAAREPGRKVVVTGCLVERYGEELAGLLPEVDAFVGRSRYGEIE
                     RVIGKSGLHLGKGGFSETFPRQVLTKPPTAYLKIQEGCDNRCRYCTIPAIRGGLTSRA
                     EGRDRGVPLAPRPGLS"
     gene            4191..4967
                     /locus_tag="PKN85_02735"
     CDS             4191..4967
                     /locus_tag="PKN85_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT43346.1"
                     /translation="MIGEFRWLLDQGYREINVIGQDITSYGKDAGREQGLTGLIRSLL
                     KEKGDWHLRLLYMHPKGITEGLIDVMGSDARVIPYLDVPIQHSEDRILSLMGRGHTRA
                     YLEETLAMMRQRIPGAVLRTSIIVGFPTETEEEFEALLSFVRRHQFDMLGAFMYSREE
                     GTAAYRMKGQIRKGVKRDRYTRLMETQVEISRTRLADLLGKTVKVIVEDVGASPKVGR
                     MLTQAPDIDGVAFVNGACSEGDIRDGKVVKTLDYDVVVEV"
     gene            4997..>5518
                     /locus_tag="PKN85_02740"
     CDS             4997..>5518
                     /locus_tag="PKN85_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNT43347.1"
                     /translation="MEPTNELYAVRKEKEKSLKEQGIDTYPQDRGPYTTTKDVEERFG
                     PQSAEDLEKNEERVAIAGRIMAFRDFGKSAFIHVQDRKGKMQAYIRKDILKSPAYDIF
                     KKFDICDIVGLEGKVFKTKTGELTVLADSIKLLTKSLRPLPEKWHGLKDVEERYRKRY
                     LDLIVNEKVRDVFT"
BASE COUNT         1200 a         1558 c         1670 g         1080 t
ORIGIN      
        1 cctccggaag atggccgaga tcagaaccac caggtacacg cccgaaagcc acaccctgat
       61 gatcgatctt accagggaga tcgaaaaaac actggcaaag accgaggaag agacatcgca
      121 atgaaagtgt ctttccttct gcagaccacg ttcgatccgc gccaaggaca cccttctctc
      181 atgccggaac gacggcaggg ggtcaggtcc cgaaaagcct tgcctgagcg gagatcagaa
      241 ctcaggccgg cagcccgctc ggttactcgc agacgtatcc ataggtgatc cagcagggct
      301 cgtaatcaca ggaggggcct tccgccaggg ctaccagctg agcgctgtag ccggcggggc
      361 agctgcaaac gccggtgtga atgttcttcg tctcgcagtg gtaggtcctg gccctgtatg
      421 tctgcagata ggacccgccg ctcgaatgcg cctcagaggc ctggccgctg tagctcgaga
      481 tgtccccgct caggcccgcg gagtcccccg agagagatgc tccggcgccg aagccaagga
      541 acgatgccgc aatggccaga gagatcagga acagcccggg cccccgcccg ttccctccgc
      601 gaaccccagt cctccctttt ttcatgcgtt cctccttgtg cgtgtcaatg tgatcgtagc
      661 atgtctgagc atattacttt cggaagaaga aaaatgaccc tctttttctg cccgatgcac
      721 gtcggtttcg ccattccaat gaggtgttaa ggcgtcagga gggccccggg gaaagatgcc
      781 ggcactgacc ccatttgcgt cgtggccggc agacgtctcc aggggagggc gggttcaggc
      841 aaaagaaaaa gggcctcgaa gggccctttt cgtgcttata aaaggggttg cggcgggaga
      901 tcagaagaag taccaggctc cgaacctgta catatcggca acgcccgtgc gatcggcatt
      961 gccggtaccg acgctggtgc cgggggtgcc gtacccgtta tagcccgaga agttccggat
     1021 ccactggata ccgaacctga tggcctgatt ggcatcccac aggaggttcg ccgcataggt
     1081 ctgtgacatg ttgatcctgt cgcgggccgt cgcgctgaag ctgcgcccgt acccgctgta
     1141 gttgaacttc aggtagccgt acatcccgtt gagcgagagg ctgtcggtga tccaccagga
     1201 cgcctgtgtg aaaagaccga aggacgtcgg ggccgtcaga tcgccggtcg gtctctgata
     1261 gcttccctcg ctcaagcccg cnnnnnnnnn ncgtaccgag ccagttgttg cccgcgatgt
     1321 tctggccgat aaagaaattg ccgttgagaa ggacggccat cgccttattg ccctttctct
     1381 cggggacgat gggtattgag tacctgaagg cggcgaccca ggaattgatc gtgtcgtccc
     1441 tgtaatcgtt gccggccgcg gtgagatacg tcgcgctcgt gccgttgtcc ttgccgtaat
     1501 acccgcccaa cgcgaacttc aggttattgg ggccgatctt gccgcagcgg tccgtccagt
     1561 acgcgatctc gccctcgaac ccgggccagc tgctcctggc gaagccgtca ttcgaaccgc
     1621 ggacgccatt ggcgtagtag ttgttggcac ccgaccattc cgtcgcggag acaaggccga
     1681 acatggcgtt gaattccctt gtgaagaaat acctgagagc gatctgaggc agcctgatcc
     1741 ccctgaggta ttccgtcacg tcgccggcgc ccatccacgc gccgtagtaa ggcataccga
     1801 actgctgcca ggtctggccg gcgatcagct cggcggattg ccatttaagc ttcatgaagg
     1861 cgtgtctcag gttgaagccg ccgtactggt tacccgttgt cacaccgcgg aaatcgcctt
     1921 cgataaaggc gctcgtcttc gcgccccaga ggtcgggacc cttgacaagg aagttgaaac
     1981 gtgtctcgcc gccgctcatg aatgtgttgc catactcgtc ggcaaggacc tttcggctgg
     2041 atgtactgct cctggccgcc gtactggcat cggcattgcc gttctgggat gtgaagccca
     2101 ggtcgaactt cacgaagccg ccgaatgtga catcgtatct gctcgacacg ctgccggcat
     2161 tgcacagaac cggaagaata agagagacca caaacaaaac cagcaaacac ttcttcattc
     2221 catcctcctt cgaatattga catgatccgg agatcttaac tttatcagcc cgtaagcacc
     2281 gggacggaac cccgatctgc acctggggat gccgaccgct atatacgtgt gtatattaca
     2341 cgtgctatat atcatgggat ataacggcag gtcaacacaa aaaaaccggt ccgagcttta
     2401 tttcggggaa agagatgtcc gggttcttat tctaaaagag gccggtttgg ccggggatca
     2461 tggggcgagg gtttcgagtg ccggggcacc tggacacatg ttgggcgcct gcgaggacct
     2521 ggcgctcatt gatgttgccc cctgtctgca ttctcctgcg ggttgtcggt ctcttcactg
     2581 cgcggggctt cgtggccgac ataaacgatc tggttgcagc aaacagcgat atacctgctc
     2641 ccgaagggaa tgaccttcac ttctttgccg caatgaggac attttttttg gtcaagcgta
     2701 ccccccgtca ggttgcgtat gaaattgatg tcttgcagaa ccaagaacca atctaccatg
     2761 gatcatggaa ggacgcgctg ctgttttatt aattagaaaa tatgggtgtg gttaccatat
     2821 aaaacggggc tgccctggcg ggacacgatt tgaaaagatc ggcccacctg gtgtacattg
     2881 ttcgcaaatc cggtccctta tgcaggggcc ttcgcgggaa ggtggtccca gatagtcgat
     2941 ctcgaaagga tctacaagag agaagggccg gcggttatcc ggcggatgga cggggacccc
     3001 gtttccttcg tccaccgctg gcaccttgac gccgacaggg agatcgccgg cttcatcgcg
     3061 gcgcagttcg cctacgggag gatcgaggtc ttcatgggct tcctcgagaa gctcttcgcg
     3121 ctgatggacg agggccccga acgcttcgtc cggcgtggcg cgtgggaggg actgaaaggc
     3181 ctgtactacc gcttccagaa ggaggacgat cttgttcacc ttttcgaggt gctgcaccgg
     3241 atcgtcggcg atcacgggtc cctgggcgcc atgttccgct ccttttacga gggcgacacc
     3301 cggcgggccg tctggcgcat ccgtgatgcc taccttgccg gtgacgacag gctcaccttc
     3361 ttctttccga ggccgtcgtc gacgggggcg cagaagcgct ggagcatgtt cctccggtgg
     3421 atggtgagaa aggacgagat agatatcggc atctgggatt tcatcgatcc ggcggacctt
     3481 accatacccc tcgacacgca catatacaag gtgggaagat gcctcggctg gaccgggcgg
     3541 aagacccctt cgtaccgggc tgcccgggag atcacggagg ccttgaagcg ctattgccca
     3601 tcggacccct tgaaatacga ctttttcctt tgccacggcg tcggcatcgg ggcggggtgc
     3661 acggggaaaa gaagcgacag atgcagggag aaatgtgccg ttcatgagat ttaggatcgt
     3721 aagcctggga tgtcccaaga acctcgtcga atcggaatac atggtgggca gactctgctc
     3781 ggcggggcac accgtctccg atgacggcga cacgatcatc gtcaacacct gcgccttcat
     3841 cgccgaggcc gcgcgggagt ccatcgagac catcctcgag gcggcccggg agccgggcag
     3901 gaaggttgtt gtcacgggat gtctcgtgga gcgctacggc gaagagcttg ccgggctcct
     3961 tcccgaggtg gatgccttcg tgggcaggtc acggtatggt gagatagaac gcgtcatcgg
     4021 caaaagcggc ctgcacctcg ggaaaggagg tttttcggaa acctttcccc ggcaggtgct
     4081 tacgaagccg ccgacggcgt atctcaaaat acaggaaggc tgcgacaacc ggtgccggta
     4141 ctgcaccata ccggccatcc gcggaggctt gacgtcgagg gccgaaggac gtgatcgggg
     4201 agttccgctg gctcctcgac cagggttatc gtgagatcaa cgtcatcggt caggacatca
     4261 cgtcctacgg aaaggacgcc ggacgggaac aggggctgac cggtcttatc cgctcactcc
     4321 tgaaggaaaa gggtgactgg catctcaggc tcctgtacat gcatccgaag gggatcacgg
     4381 aggggctcat cgacgtcatg ggaagcgacg cgcgggtgat accctacctc gatgtcccca
     4441 tccagcattc agaggaccgt atcctctccc tcatgggcag ggggcatacc agggcctacc
     4501 tcgaagagac gctcgcgatg atgaggcagc gcatacccgg ggcggtgctc cggacgtcca
     4561 tcatcgtcgg gttcccgact gagacggaag aggagttcga ggcccttctc tccttcgtgc
     4621 gccgccatca gttcgacatg ctgggcgcct tcatgtactc cagggaggaa ggcaccgcgg
     4681 cgtacaggat gaaggggcag atacgcaaag gggtaaagcg cgaccgctac acgcgcctca
     4741 tggagacgca ggtggaaata tcccgcaccc gtctcgcgga ccttctgggg aagacggtga
     4801 aggtcatcgt ggaggacgtc ggcgcgagcc cgaaggtggg caggatgctc acccaggcgc
     4861 ccgacatcga cggggtcgcc ttcgtgaacg gcgcctgttc cgaaggagac atacgcgacg
     4921 gaaaggtcgt gaaaacactt gattatgacg tcgttgtgga agtatagaaa tatatcgcca
     4981 attgacagag gatacgatgg aaccgacaaa tgagctctat gccgtgcgca aggaaaaaga
     5041 aaaaagcctg aaggagcagg gcatcgatac ctacccccag gacaggggtc cctataccac
     5101 gacgaaggac gtggaagaaa ggttcgggcc gcaaagcgcc gaagacctgg aaaagaacga
     5161 agagcgtgtc gcaatcgcgg gacgcatcat ggccttccgc gatttcggca agagcgcatt
     5221 catacatgta caggacagga aggggaagat gcaggcatac atccgcaagg acatcctgaa
     5281 gagccccgct tacgatatat tcaagaagtt cgacatctgc gacattgtgg gccttgaagg
     5341 caaggtcttc aagacaaaaa caggtgaact gactgtgctt gccgacagta taaaactgct
     5401 caccaagtcg ctgcgccccc tcccggaaaa gtggcacggg cttaaagacg tggaagaacg
     5461 ctaccgcaaa cgctacctcg acctgatcgt gaacgagaag gtccgcgatg tctttacg
//
LOCUS       DAOQTR010000072         5496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16331_length_5496_cov_4.116155, whole genome shotgun sequence.
ACCESSION   DAOQTR010000072 DAOQTR010000000
VERSION     DAOQTR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16331_length_5496_cov_4.116155"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1206)
                     /locus_tag="PKN85_02745"
     CDS             complement(22..1206)
                     /locus_tag="PKN85_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT43348.1"
                     /translation="MLTKSFILELFSAAAMQRWNDKIRPVELKELDKQAHKMTIAYFL
                     GKFEESKEGFDWLEVIEGGLFEFLERLVITDLKPQIFNRIKEDRKRYEELTKWVYKRL
                     EPVISPLGDDFLERFRRYFSSTDNTINKRIINAAHFYATRWEFGIIERANPTGYEIPE
                     IRGFLQQRQERYYDLAGIQQLALFEKYRNFLDLCGQLRFQYRWSHINMIPRTSVLGHM
                     LIVAILSYLFSLQIGACPRRCFNNYFTGLFHDLPEVLTRDIISPVKRSIEGLDSLIKA
                     YEKEQMEKEVFNLIPPEWHPEMRTFTEDEFSSIALVDGKVFKTNTETISSRYNEERFN
                     PRDGAMIKAVDDLAAYVETYCSTENGVKSPELAEARLSISEKYRNAVIGGIHFPALFG
                     QF"
     gene            complement(1510..1917)
                     /locus_tag="PKN85_02750"
     CDS             complement(1510..1917)
                     /locus_tag="PKN85_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNT43349.1"
                     /translation="MGFDKFGRKSFTSFTKTGKFVDLLGEGKVEGTVCKQCGAKYFPP
                     RADCAACLSKEMDWFELPKKGTLETFTTAMYAPFGFEADAPYTMAVVDFGGGLKAFAR
                     LAKDFSDPKIGMDVTVRPLKYDDGQLSFEITKA"
     gene            complement(1919..3100)
                     /locus_tag="PKN85_02755"
     CDS             complement(1919..3100)
                     /locus_tag="PKN85_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43350.1"
                     /translation="MAKDVAVIGVGQTYFVRGYEGSIRELAFEAFREAMQDANLTNKD
                     VDASIFCSAPEYDKQRSPAGVLAEYLGLVPQPTFYVETVCSSSTVGVKIGYSMIKAGL
                     HDIVVVLGFQKMSEITSAESQERMGRGGDIQWEAPFGTMMPAYYALYAQAYLAKNGLT
                     LDDLRDVRVKAATYGQINERAVYRKGVKPEDFDPNNPDAKMAGPVASPLRVGDCCANA
                     DGASCIILANAEKAKVFSKKPVWILGIGAASEVVNMAARPDFSKGIQVAKAAAGQAYK
                     MAGLGPKDIDVAEVHDCFTIAEIMAYEGLGFAPEGDGKQLIREKATYKEGRIPVNVDG
                     GLLSKGHPIGATGGSQIRTVVLQLRDEAGPMQVKDPQIGLNHNIGGVGLYGNVTIFGR
                     D"
     gene            complement(3115..4113)
                     /gene="mltG"
                     /locus_tag="PKN85_02760"
     CDS             complement(3115..4113)
                     /gene="mltG"
                     /locus_tag="PKN85_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNT43351.1"
                     /translation="MLAARDKKTIVIAIIIFIFSQGLIFACIPRDNDRSIRPVVVKQG
                     TGVAEIANALKKEGIIRSSALFMIGSLMYRGHLIAGEYELTRDMSTFEIIRKMGQGKR
                     KIYVLKIIEGNNIYTIAESVGRSGIMTREEFLELSTDKKYLESMGIEADSMEGYLSSN
                     TYYYSKEIDREKFLKKIALRTLKLFSNEDIRRKMASLGMNTHQTLTLASMIEKESGAK
                     DEKPLVSAVFHNRILKGMSLDCDPTVIYGTKGFGSPIRKIDLATRTPYNTYMLKGYPR
                     GPICTPSKDSIMAALNPAPVDYLYFVSKNDGTHVFSRDMAEHNRYVEMYQRNRTTQ"
     gene            complement(4094..4510)
                     /gene="ruvX"
                     /locus_tag="PKN85_02765"
     CDS             complement(4094..4510)
                     /gene="ruvX"
                     /locus_tag="PKN85_02765"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNT43352.1"
                     /translation="MRILGLDVGDKKIGVSLSDPTLSIAQGLKLYRRASLEEDVEELK
                     RIVEEHEVGEIVVGLPRNLNGTIGTRAEKVMGLADRIRESVTIPVTLWDERFSTNEAH
                     RVFDMAEVRHKKRKPYLDIMASQIILQGYLDARSAR"
     gene            4759..5205
                     /locus_tag="PKN85_02770"
     CDS             4759..5205
                     /locus_tag="PKN85_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT43353.1"
                     /translation="MEFTTGPVKLKVLTLKDLDAVTDIDESLLGTRRRDYWEVRLERA
                     EVSGVPSLAAELDGRVIGFILGSASGWEYGIPENVAWIDTLGVRKEYQKQGIARLLFQ
                     EMFSMFKKVGVDTIYVFVNWRDWDLLRFFDRMGFKRGDMINLEMKI"
BASE COUNT         1091 a         1511 c         1562 g         1332 t
ORIGIN      
        1 acctgggacc tgagaccgtt cttagaactg tccgaaaagg gcggggaagt ggatgccccc
       61 tatcactgca ttcctgtatt tctcgctgat ggacaggcgg gcttcggcca gttccgggga
      121 cttgacgccg ttctccgtgg aacagtatgt ctcgacatac gccgcgaggt cgtctacggc
      181 ttttatcatc gccccgtccc ttgggttgaa acgctcttca ttgtagcggc ttgatatcgt
      241 ctcggtgttg gtcttgaaga ccttgccgtc gacgagtgcg atgctgctga actcgtcctc
      301 cgtaaaggtt ctcatctccg ggtgccattc gggcgggatg aggttgaaga cctccttctc
      361 catctgctcc ttctcgtagg ccttgatgag ggagtcgagg ccttcgatgg agcgcttgac
      421 gggcgatatg atgtcccggg tgaggacctc aggcaggtcg tggaagaggc ccgtgaaata
      481 gttgttgaag cagcgcctgg gacaggcgcc gatctgaagg gagaagagat aggaaaggat
      541 ggccacgatg agcatgtggc cgaggaccga tgtgcggggt atcatgttga tgtggctcca
      601 ccggtactgg aaacggagct ggccgcaaag atcaaggaag ttccggtact tctcgaagag
      661 ggcgagttgc tggatgccgg cgagatcgta gtagcgctcc tgcctctgct gaaggaagcc
      721 cctgatctcc ggtatctcat agcccgtcgg gttggcgcgc tcgatgatgc cgaattccca
      781 ccgcgtggcg tagaaatggg cggcgttgat gatccgcttg ttgatcgtgt tgtcggtgct
      841 tgaaaagtag cgccggaagc gttcaaggaa gtcgtcgccc agaggggaga tgacgggttc
      901 caatctcttg tacacccatt tcgtcagttc ctcgtagcgc ttcctgtcct cttttatcct
      961 gttgaatatc tgaggtttga ggtctgttat gacgagcctt tccaggaatt cgaagagccc
     1021 tccctcgatg acctccagcc aatcgaaccc ttctttcgac tcctcgaact tgccgagaaa
     1081 ataggcgatc gtcatcttgt gcgcctgctt gtccagctcc ttgagctcca cgggccgtat
     1141 cttgtcattc caccgttgca tggccgcggc gctgaacaat tccagtatga acgatttggt
     1201 gagcatcaca gacttcattg taaaggaatc gggaagaaaa ggaaaggggg aaagggggcg
     1261 ggatgcggga cgcagggtcc tgacaagacc gataggacgc ttaaggacct ataggacggg
     1321 ttgttgcccg ctgacggcgg caactggctg cagaacgttc ctataagtcc tatctgtcct
     1381 atacgtccaa tatgtcctat aggtcctatt tttctattgg tcctatcagc cccccctttc
     1441 cactgatccc tctcctctgt ccaataaaaa aggccgcccg cgggcggcct tttttattcc
     1501 atttctcgtt tacgcctttg ttatctcaaa gctgagctgg ccgtcatcgt acttgagggg
     1561 tcgtaccgtg acatccatac ctatcttggg atcactgaag tcttttgcca gacgggcgaa
     1621 ggccttgagg ccgccgccga agtccacgac ggccatggta tagggcgcgt cggcttcgaa
     1681 accgaagggg gcgtacattg cggtggtgaa ggtctccagg gtgcccttct tcggcagttc
     1741 gaaccaatcc atttccttcg aaagacaggc tgcgcagtcg gcccgcggag ggaagtactt
     1801 cgctccacac tgcttgcaga ccgttccttc caccttgcct tctccaagga gatcgacgaa
     1861 tttacccgtt ttggtgaaac tggtgaagct ttttcttcca aatttatcga atcccatctt
     1921 agtccctccc aaaaattgtg acgtttccgt agaggcctac gccgccgata ttgtgattga
     1981 ggccgatctg aggatctttg acctgcatgg ggccggcttc gtcccggagc tgaaggacaa
     2041 cggtccggat ctgtgaccca ccggtggctc cgatcggatg gccttttgag aggaggccgc
     2101 catccacgtt cacggggatc ctgccttctt tgtaggttgc tttctcgcgg atgagctgct
     2161 tgccgtcgcc ctcgggcgcg aatccgaggc cttcgtaggc catgatctcc gcgatggtga
     2221 aacagtcatg gacttcggcg acatcgatat ctttgggtcc cagccccgcc atcttgtatg
     2281 cctggcctgc cgccgccttc gcaacctgga tgcccttgga aaaatcaggt ctggcagcca
     2341 tattgacaac ctctgacgcg gcccctatgc caaggatcca taccggcttc ttcgagaata
     2401 ccttggcctt ctcggcgttt gcgagaatga tgcatgacgc gccatccgca tttgcgcagc
     2461 agtcgcctac gcgaagcggc gaggcgacgg gtcccgccat cttcgcatcg gggttgttgg
     2521 gatcgaaatc ctcgggtttc acgcccttgc ggtagacggc cctttcgttg atctggccgt
     2581 aggtggccgc ctttaccctg acatccctca agtcgtcgag ggtgaggccg ttcttggcaa
     2641 gatacgcctg ggcgtagagc gcgtagtagg cgggcatcat cgtgccaaag ggggcttccc
     2701 actggatgtc gccgccgcgg cccattctct cctgggactc ggcggaggtg atctcggaca
     2761 tcttctggaa gccgagaacg acgacgatgt catggagacc ggccttgatc atgctgtagc
     2821 cgatcttgac gccgaccgtg ctggatgaac acaccgtctc gacgtagaat gtcggctggg
     2881 ggacaaggcc gaggtactcg gcgagcacgc ccgccggcga tctctgcttg tcatactccg
     2941 gggcagagca gaagatggag gcgtccacgt ccttgttggt gaggttcgcg tcctgcatcg
     3001 cttctctgaa ggcttcgaag gccagttccc tgatggagcc ctcatagccc ctgacgaagt
     3061 acgtctggcc aacgccgata actgcaacat cttttgccat acattcctcc tttattattg
     3121 tgttgtcctg tttctctggt acatttcgac gtatctgtta tgctctgcca tgtcccggga
     3181 aaagacatgg gtaccatcat tctttgagac aaagtaaagg taatcgacgg gtgccgggtt
     3241 gagcgcggcc ataatggagt ctttcgacgg ggtgcatatc ggcccccggg ggtagccttt
     3301 gagcatgtat gtgttgtaag gagtgcgcgt cgcgaggtcg atcttccgta tgggggatcc
     3361 gaagcccttc gttccgtaaa tgaccgtggg gtcgcagtcg agcgacatcc ccttgagtat
     3421 cctgttgtgg aagacggcgg agacgagagg tttctcgtcc ttggctcccg attccttctc
     3481 gatcatcgaa gcgagggtga gtgtctggtg cgtgttcatc ccgagggaag ccatcttccg
     3541 cctgatatct tcgttgctaa agagcttgag ggtcctcagc gcgatctttt tgaggaactt
     3601 ctccctgtcg atctccttac tgtaataata tgtgtttgaa gaaagatatc cctccataga
     3661 atcggcttcg atgcccatgc tttccaggta cttcttgtcc gtggagagtt cgaggaattc
     3721 ttctctcgtc atgatgccgc ttctgccgac ggattccgct atcgtgtata tgttgttgcc
     3781 ctcgatgatc ttcaggacgt agatcttccg cttgccctga cccatcttcc gtatgatctc
     3841 gaacgtggac atatccctcg tgagttcgta ctcgccggcg atgaggtgac ccctgtacat
     3901 aagagatccg atcatgaaga gggccgatga acgtatgatg ccttccttct tgagggcgtt
     3961 cgctatctcc gccacgccgg ttccctgttt cacgacgaca ggccgtatcg accggtcgtt
     4021 gtccctgggg atgcaggcga atatcaagcc ctgcgaaaaa atgaagataa tgatggcaat
     4081 gacgatggtc ttcttatcgc gcgctgcgag catcaaggta tccctgcaga attatctgtg
     4141 atgccatgat atccagatac ggttttctct tcttgtggcg cacctcggcc atatcaaaga
     4201 ccctgtgcgc ctcattcgtg ctgaaacgct catcccagag ggtaacgggt atggtcaccg
     4261 actcgcggat cctgtcggca agccccatga ccttctccgc ccgtgttccg attgtgccgt
     4321 tgaggttcct gggaaggccc acaacgatct ccccgacctc gtgttcctcc acgatacgct
     4381 tcaactcttc cacatcctct tcaagggaag cgcggcgata gagtttcagg ccctgggcga
     4441 tcgacaaggt gggatcggac aaagacaccc cgatcttctt gtcccccaca tccagcccga
     4501 gtatacgcat caactattat agcagttttt gctttcattt caagaacaaa actacccggc
     4561 aggatgtcgc cggacgacgc gctctgcagg caaagaccct cgcagggaac aggaaagatc
     4621 agaggcaaag gcggggatag gaccaatgag acctattggt cctgctcttc cctctctccc
     4681 cgtcctgtgt tgacaagtcc cctttctctg ctatactcca cagtaagaaa gtaagttgcg
     4741 cgggggaggc atagagccat ggaattcacc accggacccg tgaagctgaa agtgctcacc
     4801 ttgaaggacc tcgatgccgt caccgacatc gacgaatcac tcctcgggac aagaagaagg
     4861 gattactggg aggtaaggct tgagagggcc gaagtgtcgg gggtgccatc gcttgcggcc
     4921 gagcttgacg gtcgggtcat cggtttcatt ctcggcagcg cgagcggctg ggaatatggt
     4981 atacccgaga atgtcgcgtg gatagacact ctcggggtca ggaaggagta ccagaagcag
     5041 gggatcgcga ggcttctctt ccaggagatg ttctccatgt tcaagaaggt cggcgtcgac
     5101 acgatctatg tcttcgtgaa ctggagagac tgggaccttc tcaggttctt cgaccggatg
     5161 ggttttaaga ggggagacat gatcaacctg gagatgaaga tctgaaagag tctttaacgg
     5221 gccgactctc gccggcccag cggtatagac cgcagaagac cggggccaaa agggatccac
     5281 cagccccggc cttcttgtcc ttaagcccat atcggaccaa tcccttcgtt gtcttcgtcg
     5341 tcggcatcct cgacgtacgg acgtacgcct ccggcgcctt ctcctcgccg cctcggtatt
     5401 gccccgatct gggcttaagg acaagagagg tggcttgaat ggcttgacag cctgttgata
     5461 aactctgtct cacggcatga tcccgctcgg tggaga
//
LOCUS       DAOQTR010000073         5460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16470_length_5460_cov_3.522479, whole genome shotgun sequence.
ACCESSION   DAOQTR010000073 DAOQTR010000000
VERSION     DAOQTR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16470_length_5460_cov_3.522479"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKN85_02775"
     CDS             <1..375
                     /locus_tag="PKN85_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HNT43354.1"
                     /translation="VSDFKTPRGYLKISTPAATAFDIVGYAHHAGGLDNVATILAELA
                     EKIGSDELVTVAALSPIAWSQRLGYLLDLVGAEEKTGELARYIDQKNVLPTPLVPAQP
                     FKGIDQLKRWRILVNAKVEVEI"
     gene            468..1235
                     /locus_tag="PKN85_02780"
     CDS             468..1235
                     /locus_tag="PKN85_02780"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNT43355.1"
                     /translation="MEMFNHPLLSENLAFRGGTALFKLYLEPLRYSEDIDLVQVHAAS
                     IGPVMDAIQDKLNPWLGPPKRSRSEGRVTLIYRAQSEEGLPLKLKVEINSREHFAVLG
                     FEKRRFDVNSRWFSGSAFISTYHLDELLGTKVRALYQRKKGRDLFDLWTAFRTTDARP
                     ERVIHCFLQYMENEGHKVSRAEFEQNLIEKRDGPHFLDDIGPLVVAENGWDPDGAMEF
                     ALNRLAVLIPGEPWQGRTKKAKGPKKIIGSGHPAGGE"
     gene            1235..1804
                     /locus_tag="PKN85_02785"
     CDS             1235..1804
                     /locus_tag="PKN85_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNT43356.1"
                     /translation="MKLLIRGGSVAAGYGATRRYADILAERLLPRGVEVIVRSRHRET
                     TFDGVATFAVDIDPFRPDILLFNFGVDDAFQGVYRSEFQENLVQMIRLARLRFGPQVV
                     LATSHTFDDPYDMDAVNIFYRSLSIVASDLDCLLVPVHHHFAGYLEEKGLTSKDLTGS
                     DPRYPNDRGHGVIAEAIIEGIDRLIPASF"
     gene            1961..2872
                     /locus_tag="PKN85_02790"
     CDS             1961..2872
                     /locus_tag="PKN85_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT43357.1"
                     /translation="MIRSRFSLPSIHLFLCVAFLLLFMSVFPIADSSAVTLEEIRRNG
                     VLRHLGIPYANFVTGSGDGMDVELMSLFAERLGVRYEFVRTDWDRVFADITGKTFTRG
                     DGNVIITGDAPVKGDVAASGITVLPWREELVDFSLPVFPTQVWLMARADSSVKPVRPT
                     GDLNRDVARTKELVRGRSIMGKTGTCLDPSLYGLEKTADRVRPYGGSLNEIAPALMEG
                     VADLVLLDVSDALVALQKWPGRIKVIGPVSEMQEMAVAFRKDSPELKDEFNRFLADCK
                     RNGTYTRIVKKYYPFVLEYHPAFFRRK"
     gene            2884..>5460
                     /locus_tag="PKN85_02795"
     CDS             2884..>5460
                     /locus_tag="PKN85_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43358.1"
                     /translation="MAGHKGLLRSPSVRIIGVAVVGVPLVLAILFLLYSHFQTEKDLK
                     ENLLRVRHHTVERRAAALSYFFADRMDDLFYLKHSREVAAYFENRALGMSMEYGLKQS
                     LVPMRELFISLMKRKQIGDDRVYARLAYIADDGRSLVDTASRETGRGMADILKKARSS
                     GGAGKISMSRDGEHFMVISAHEYKGEHRGHVVGWIRPSIIHRHILAADESAQSSYLIC
                     DERGDPLLMKDIVVAGPTHLDILRSLPDGGHREFSVNGTTGSQMDVVAYRMPVPDTGY
                     SLVHLCPAEKIYGTLASRDRIATISLLVIALLAGVAFTVRSMMLKTSVDGLERRVKER
                     TADLYRLNESLQQEISERELAMRALEKAERKYRSIFENAVEGMFQTTPDGRIISVNPS
                     LASMHGYGSPREMVRELAGMAKRLYVDEGRRAEFVRLMEEDGAALHFEAQTHRKEGTM
                     NWVSINARNVRDVKGKTLCYEGSVENITERKHLESQLLHSQKMEAIGTLAGGIAHDFN
                     NILTAIVGYGNLLQLKMEKDDPLRTYTDRILSSSQKAINLTRSLLAFGRKQPVELRPH
                     RVGTLIEGSGKLLKRLLTEDIALRMGRVDGDAVIMADRTQMDQVLMNLATNARDAMPE
                     GGTLAVEAERVVMDNDFVRTHGYGSPGDYVLIRVADTGAGMDEGTMKKVFEPFFTTKA
                     EGKGTGLGLSIVYGIVKQHNGYITIDSEPGGGTTFNMYFPEAKTGETVDSEAAQVPLN
                     IRVGSETILVAEDNAEARSFLTEVLSVSGYTVIEAVDGDEALRAFSDNKDRIALVVSD
                     VVMPGRNGGELREEIQKIKPDMKILFTSGYARDVLLFKGIHDKAANYISKPLSPNDLL
                     GKV"
BASE COUNT         1235 a         1503 c         1640 g         1082 t
ORIGIN      
        1 gtatcggatt tcaagacccc gcgcgggtat ctgaagatat caaccccggc ggcaaccgct
       61 tttgatattg tgggatacgc ccatcatgcg ggtggcctgg acaatgtggc gaccattctg
      121 gcggaacttg cagaaaagat cggaagcgac gaactggtca ccgtggcagc gctttcaccg
      181 atagcctggt cgcaacggct cggatacctt cttgaccttg tcggggcaga agagaaaacc
      241 ggggagttgg cccgatatat cgatcaaaag aacgtactcc ccaccccact cgttccagcg
      301 caaccgttca aggggatcga tcagctaaaa cgatggcgaa ttctcgtgaa tgctaaggtg
      361 gaggtggaga tctgatacct ctcgatttta tatctgagtg gaagaaatat actccatggc
      421 cccagagcta tcagatcgaa caggacctca taatttgcag ggctctggtg gaaatgttca
      481 accatcctct tttgtccgaa aaccttgctt tccggggagg aactgcgctt ttcaagctgt
      541 atctggaacc actgcgttac tcggaagaca ttgatctggt acaggtccat gccgcatcca
      601 tcgggccggt aatggatgcc atacaggaca agctcaatcc ctggcttggc cctcccaaac
      661 ggagccgctc ggaaggcagg gtaacattga tctacagggc acaatcagaa gaagggctac
      721 ccttgaaact gaaggtggaa atcaattccc gggagcattt cgcggtactc ggatttgaaa
      781 aacgtcgctt cgacgtcaat tcacgctggt tctccggcag tgccttcata tcgacctatc
      841 atcttgatga gcttctgggg accaaggtcc gggcgctcta ccagagaaag aaaggccgcg
      901 acctctttga cctgtggaca gcattcagaa ccactgacgc ccggccggag cgggtaatcc
      961 attgtttcct gcaatatatg gaaaatgagg ggcacaaggt atcccgcgct gaattcgagc
     1021 agaaccttat tgagaaacgt gatggtccgc actttctcga tgatataggc ccacttgttg
     1081 tcgcagagaa tggctgggac cctgacgggg caatggaatt cgccctcaat agactggcgg
     1141 tccttatccc cggggaaccg tggcaaggaa gaacgaagaa agcgaaagga cccaagaaga
     1201 tcataggttc gggccatccg gcagggggtg agtgatgaag cttcttatcc gcggcgggag
     1261 tgttgccgcc ggttatgggg cgaccaggcg ttacgccgat atcctggcgg agcgccttct
     1321 cccgagaggg gtggaggtca tcgtgaggtc gaggcacagg gagaccacct tcgacggcgt
     1381 cgccacgttc gctgtggata tcgacccctt ccggcctgac atccttcttt tcaatttcgg
     1441 cgttgacgat gcgtttcagg gcgtctatcg ctcggaattc caggagaacc tggtgcagat
     1501 gatacgactg gcgcggttgc ggttcggtcc ccaggtcgtc cttgccacat cgcatacctt
     1561 cgacgacccc tacgacatgg acgccgtcaa catcttctac cgctccctca gcatagtcgc
     1621 ctccgacctt gactgccttc tggtcccggt gcaccatcat ttcgcgggct acctggaaga
     1681 gaagggcctc acgagcaaag accttacggg gtccgacccg agatacccca acgacagggg
     1741 ccacggggtc atcgccgaag cgatcatcga gggcattgac agactcatcc cggcgtcatt
     1801 ctgacggtcc cttaatattt ccagacaacg agcgattatt cctgtacggg cgtttattcc
     1861 atctggcgag aacattcatt cggaaagtgt acggaatatc atgttgaaag acgggttacg
     1921 ccagcggcac agcgacttct cgaccgggca gggtcaccgg atgatccggt cccgcttcag
     1981 tcttcccagc atccatttgt tcctttgcgt ggcattcctg cttttgttca tgtctgtttt
     2041 tcccatcgcc gattcctccg ccgtcaccct ggaagagata aggaggaatg gtgtcctgag
     2101 gcacctcggc ataccctatg ccaatttcgt caccggcagc ggggacggaa tggacgtcga
     2161 gctcatgtcg ctcttcgcgg agcgtctcgg ggtaagatat gagttcgtga gaacggactg
     2221 ggaccgcgtc ttcgccgaca tcacgggaaa gacgttcacc cggggtgacg ggaacgtcat
     2281 catcaccggt gacgcgccgg tcaagggtga tgtggcggcg agcggtatca ccgtccttcc
     2341 ctggagagag gaactcgtcg atttttccct tcccgtgttc ccgacccagg tctggctcat
     2401 ggcccgcgcc gattcgtccg tgaagccggt gagaccgaca ggtgacctga accgcgacgt
     2461 tgcccggacg aaagagctcg tcagggggcg ctcgataatg ggaaagacag ggacctgtct
     2521 tgacccttcc ctgtatggac tggagaaaac ggcagaccgg gtgaggccgt acggcgggtc
     2581 actgaacgag atcgcccccg cgctcatgga gggtgttgcc gacctcgttc tccttgatgt
     2641 ttccgatgcg cttgtggcgc tgcagaagtg gcccggccgt ataaaggtca tcggtcccgt
     2701 gagcgagatg caggagatgg ccgtggcatt caggaaggac tctccggaac tgaaggatga
     2761 gttcaacagg tttctggccg actgcaagag gaacggcacg tacaccagga tcgtgaagaa
     2821 atactatccc tttgttttgg agtatcaccc cgcgttcttc agaaggaagt gatgaggacc
     2881 acgatggcag gccacaaagg actactcaga agcccgtccg tgcggatcat cggagtggcc
     2941 gtcgtgggtg tgccgctcgt tctcgcgatc ctcttcctcc tttacagcca tttccagacc
     3001 gagaaggacc tgaaagagaa ccttctcagg gtccgccatc acaccgtcga gcgacgggcg
     3061 gctgcgctga gctatttctt tgccgacagg atggacgacc ttttctacct gaagcattcc
     3121 cgggaagttg ccgcgtactt tgagaacagg gcactgggga tgagcatgga atacggcttg
     3181 aagcagagcc ttgtgcccat gcgggagttg ttcatctccc tcatgaagag gaagcagatc
     3241 ggggacgaca gggtctatgc tcgacttgct tacattgccg atgacgggag atccctcgtg
     3301 gatacggcat cgcgggaaac gggcaggggc atggccgaca tattgaagaa ggcgcgttct
     3361 tccggcggcg ccggaaagat atcgatgtcc cgggatggtg agcacttcat ggtcatatcg
     3421 gcacacgaat acaaaggcga gcaccgggga cacgtcgtgg ggtggatcag gccgtcgata
     3481 atccacaggc acatactcgc tgcggacgaa tccgcgcaat cgtcatacct catctgtgac
     3541 gagcggggag accccctgct catgaaggat atcgtcgttg ctggccccac gcacctcgat
     3601 atcctgaggt cgctgcctga cgggggacac cgggagtttt ccgtgaatgg gacgacgggc
     3661 agtcagatgg acgtggtcgc ctacagaatg cctgtccccg acacaggcta ttcgctcgtc
     3721 catctgtgtc ccgcggaaaa gatctacgga acgctggcga gccgcgaccg tatcgccacc
     3781 atatccctgc tcgtgatagc tcttttggcg ggggtcgcct tcaccgtgag gtccatgatg
     3841 ctcaagacga gtgtcgacgg actcgagagg cgggtcaagg aacggaccgc cgatctctac
     3901 cgcctcaatg agagcctcca gcaggagata tccgaacggg agctggccat gcgggcgctg
     3961 gagaaggcgg aaaggaagta ccgctccatc tttgagaacg ctgtggaagg gatgttccag
     4021 acgacgccgg acggacgcat catcagcgtc aacccctctc tggcctcaat gcacggatac
     4081 ggctcaccgc gggagatggt ccgggaactc gccggtatgg caaagcggct ctatgttgat
     4141 gaagggcgcc gggcggaatt cgtccggttg atggaggaag acggggccgc gctgcacttc
     4201 gaggcgcaga cccaccggaa ggaagggacc atgaactggg tctccatcaa tgccaggaat
     4261 gtgcgggacg tgaagggtaa gacactctgt tacgaggggt ccgtcgaaaa catcaccgaa
     4321 cggaagcatc tcgaatcaca gcttctgcac tcacagaaga tggaggccat aggcaccctc
     4381 gccggcggga tcgcccacga cttcaacaac atcctgacgg ccatcgtcgg ctacggcaac
     4441 cttctccagc tcaagatgga aaaggacgat cccctgagga cgtacacgga caggatactc
     4501 tcctcatcgc agaaggcgat caacctcacc cggagcctgc ttgccttcgg caggaagcag
     4561 ccggtggaac taaggccgca cagggtgggc accctgatag aagggtcggg aaagcttctg
     4621 aaaagactcc tcacggaaga catagcgctc aggatgggcc gcgttgacgg tgacgcggtg
     4681 atcatggccg acagaacaca gatggaccag gtgctgatga acctggccac aaatgcacgc
     4741 gatgccatgc ccgaaggagg gacgcttgcc gtcgaggccg agagagtggt gatggataat
     4801 gatttcgtgc gcactcacgg atacggttcc cccggagact acgtgctcat ccgtgttgca
     4861 gacacgggag ccggcatgga cgaagggacg atgaagaagg tgttcgagcc gttcttcacc
     4921 accaaggcgg aaggcaaggg aacgggtctg ggtctgtcca tcgtctatgg catagtcaaa
     4981 cagcacaacg gttatatcac catcgacagc gaacctggcg ggggaacgac attcaacatg
     5041 tactttccgg aggcaaagac cggggagacc gtggacagcg aggcggcgca ggttcccctg
     5101 aacatacgcg tggggagcga gaccatcctt gtcgccgagg acaacgcgga agcgcggagc
     5161 ttcctgactg aggtcctcag tgtctccgga tataccgtca tagaggccgt ggatggggat
     5221 gaggcccttc gcgcattcag cgacaacaag gacaggatcg cgcttgtcgt ttccgatgtc
     5281 gtcatgcccg gcaggaacgg cggagagctt cgcgaggaga tacagaagat caagccggac
     5341 atgaagatcc tcttcaccag cggatacgcc cgggatgtcc tgctcttcaa gggaatacac
     5401 gacaaggcag ccaactacat ttcaaagccg ctatcaccca atgacttgct gggaaaggtc
//
LOCUS       DAOQTR010000074         5406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_16679_length_5406_cov_3.785648, whole genome shotgun sequence.
ACCESSION   DAOQTR010000074 DAOQTR010000000
VERSION     DAOQTR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16679_length_5406_cov_3.785648"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKN85_02800"
     CDS             <1..651
                     /locus_tag="PKN85_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT43359.1"
                     /translation="GYGRINVPFADRQITEITCHAKGLHSLLPSVKTVVDIGGQDSKG
                     IKIENGKVMNFVMNDKCAAGTGRFLEIIADSLGVPLEKIGELSLTAQKIAPVSSTCTV
                     FAEQEIISRLAGGEPIANLMAGIHDAVATRVYSLVKKLNVEPDIAVTGGGAKNIGLVR
                     ALESKFGQKLLVPPEPLITGALGAALMGKDIFEKYAAEGQKPTRSGHKLEEATFFK"
     gene            662..1435
                     /locus_tag="PKN85_02805"
     CDS             662..1435
                     /locus_tag="PKN85_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT43360.1"
                     /translation="MAYVLGIDVGSGYSKAVVLEGDEVRSWAVIPSGGDYREAGRAVA
                     QTALEKVNLTLSDVAWIVAAGYGGSMVDIAGETSTDLSCHAIGVHRLFPSVRTVIDVG
                     AQYSRAIRLHDEGKIANFIMNEKCAGGSGKFLQVAARILHMNVSDIGELSLKAQKPVE
                     FTTGCAVFAESEAVSRISEGASPADILAGIHNAMASKIVTLAMRVGLTPDYAVTGGGA
                     MDTGLVKAIAAELGTNVLIPQVPQITAALGAALIAKEKV"
     gene            1767..2333
                     /locus_tag="PKN85_02810"
     CDS             1767..2333
                     /locus_tag="PKN85_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43361.1"
                     /translation="MKKSTVLLGTALIVVCVAIALSQVPARAAGPLDACSLLTKAEAE
                     ALLNQASSSQKAGKVSAPAGNQCAYFFKKKGGTYSAKVKVSSSEEIKSEGIFKSARDV
                     FDRQKRARMANADTAQKMRSLGGLGDEAFWNGYDLWIVKGGYLLTILVQDQLAGSFKS
                     REAMDKARAEQDFNLSQRAASVILLKIK"
     gene            2357..3271
                     /locus_tag="PKN85_02815"
     CDS             2357..3271
                     /locus_tag="PKN85_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43362.1"
                     /translation="MTRKVLLGSLLLLFVVAVAAAEGPQGLKIVSVKPVKSGIYAIAS
                     VEFPKAWTDPGFTVEVNGKPVRMRQVGGGFSGDRNVADLMFMPGRGARQSVAVKCLVG
                     GRKTEAKTGFDWTATPFVAVPGHTGNREVITAKEKLTIAAVNVTGMRIFFNGKEVHAR
                     SSMRGDIETFTFEPAWKKGRNVLAINADKWDSGMIARNFTFFDLGEGGTLSPGQSALV
                     HLGREGTKSGPFYDVKVEGDSIAPPQGTTVRRDIIDEDGWLVSETWVAVELRARKEGT
                     AKVRVFVKPHFLRNMELEREFLITVGEG"
     gene            3377..3574
                     /locus_tag="PKN85_02820"
     CDS             3377..3574
                     /locus_tag="PKN85_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43363.1"
                     /translation="MAMIDEKKIRAYHSRSDNALVCPLCATDEERESAEPGDLVTEDV
                     IHDDNPMYCARCKKKIEKQGS"
     gene            complement(3595..>5406)
                     /locus_tag="PKN85_02825"
     CDS             complement(3595..>5406)
                     /locus_tag="PKN85_02825"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNT43364.1"
                     /translation="EVGKVLGLPAHILDRMAKTISAYGGRAAIDSLKEVPEFASYLDS
                     GAWEHFGDLCTKIADFPRHLSIHVGGMVLTPRPIYEIVPQEHARAEGRIVCQWDKDSV
                     DDAGLIKFDLLGLRMLSLIDDAKELIREQKGLDLNLDSISPDDPEVYRMIGDADTVGV
                     FQVESRAQMQTLPKVRPRSFEDLTMEVAIVRPGPLQGNMVHPFIRRRQGKEPVVHLHP
                     KLAPILDETLGVILVQEQILQVAMAIAGFSAGEANMLRKAMGRKNARDELAKWHGRFV
                     EGAEQQGIARGLATKIFDHISGFADFGFCKSHAASFAILCYRSAYLKRYYPAEFYCAL
                     LNNQPMGFYAPEVITGDAKRHNVPILPVDINRSMWNCSIEEGSVRIGFRYVKGIGKDK
                     GDRIIKARREDIFTSLEDFADRVRLDDRSVRNLVAVGAFSDIKRSRRQLLWEAGTLRA
                     MGPAGFASLAREDRQENLPIPEMSTAEETITDYAFQGFSASHHIMKLYRKALDSLGAV
                     TSGVLPRCPPGSTVLTAGYMVCLQMPPTAKGGFSFMTLEDEEGLINVVVRPEVYRMHR
                     TTIRLEPFLLVEGVVEKKDGLINVRAKRFGNLRQRRG"
BASE COUNT         1154 a         1485 c         1687 g         1080 t
ORIGIN      
        1 ggatacggca ggataaacgt tccctttgca gaccgacaga tcactgagat aacctgccac
       61 gcgaaaggct tgcacagcct cctcccctct gttaaaaccg tcgtcgatat cggcggtcag
      121 gacagcaaag gcatcaagat tgagaacggc aaggtgatga actttgtcat gaacgacaag
      181 tgcgccgccg gtacggggcg ctttctcgag atcatagcgg attctctcgg cgtgcccctc
      241 gaaaagattg gcgagctatc actgacggcg cagaagatcg cgcccgtgag cagcacgtgc
      301 accgtgttcg ccgagcagga gatcatctcc cggctggcgg gtggagagcc catcgcgaac
      361 ctcatggcgg gcattcacga cgcggtggcc acgagggtct attctctcgt caagaaactg
      421 aacgtggaac ccgatatcgc cgtgacggga gggggagcga agaacatcgg tctcgtcagg
      481 gcactggagt caaagttcgg gcagaagctt ctcgttccgc cggaacctct cataacgggg
      541 gctttaggag ccgccctcat gggcaaggat attttcgaaa aatacgccgc ggaggggcag
      601 aagccaaccc gaagcggaca caaactggaa gaggcgacat tcttcaaata aggattcgaa
      661 cgtggcgtat gtgcttggca tagatgtagg ttcaggttat tcgaaggcgg ttgtcctcga
      721 aggggacgag gtccgttcgt gggccgtcat accgtcgggc ggcgactacc gtgaagcagg
      781 cagggccgtg gcccagacgg ccctcgaaaa agtgaacctc accttgagcg atgtggcatg
      841 gatcgtggca gccggctatg gcggcagcat ggtcgatata gccggggaga catccacgga
      901 cctatcctgt catgccatcg gagtgcaccg tctttttcct tcggtaagga cggtcatcga
      961 cgtgggggca cagtactccc gcgccatacg tctccatgac gaggggaaga tagccaactt
     1021 catcatgaac gaaaaatgcg ccggcggaag cggaaagttc ctgcaggttg ccgcacgtat
     1081 tcttcacatg aacgtgagcg acattgggga gctttccctg aaggcgcaaa aaccggtcga
     1141 attcaccacg ggatgcgcgg tcttcgccga atcggaggcg gtgtcgcgca tatcggaagg
     1201 tgcgagcccc gccgatatcc tggccgggat ccacaatgcc atggcgtcga agatagtcac
     1261 cctggccatg cgcgtggggc tcacccccga ctacgccgtt acgggcggcg gtgccatgga
     1321 tacagggctc gtgaaggcca tagcggccga attgggaaca aacgtcctca ttccccaggt
     1381 gccccagata acagcggccc tcggcgccgc actcatagcg aaagaaaaag tttaagccgc
     1441 ttgaactgct tgaaccgctt gagcggtcaa aggcgaaaaa gacggcttcg caatgcggct
     1501 ccatgtctca aggacctgtt gatgcccgac ctcttttcac ggttttttcc tgaaacctga
     1561 aacctgaaac ctgaaacctg aaacctgaaa ccctcttctg tttctgcaac ccgcaacccg
     1621 caaccgtctc ctgcccctga aacgaacacg tggtgtcacc cgcgtcaccc tcctctttaa
     1681 gtgtgtgcaa tttgtagtgg tatcgggtat attcttattg ttcgaacaat tccagcggct
     1741 gcaaagaatc ttgcgaggga ggaatcatga agaaaagcac tgtccttttg ggtacggccc
     1801 taattgtggt atgtgttgcg atcgcgttga gtcaggtccc ggcgcgcgcg gcgggtcccc
     1861 tggatgcctg cagtctgctt accaaggctg aggcggaggc gcttctcaat caggccagtt
     1921 catcgcagaa ggccggcaag gtgtccgccc cggcagggaa ccagtgcgcc tatttcttca
     1981 agaagaaagg cggcacctac agcgcgaagg tgaaggtctc ctcctccgaa gagatcaaga
     2041 gcgaagggat cttcaaatcc gcgcgggacg tcttcgacag acagaagagg gcccgtatgg
     2101 ccaatgccga tacggcgcaa aagatgagga gcctgggcgg gctgggcgat gaggccttct
     2161 ggaatggcta tgacctctgg atcgtcaagg gtgggtacct tctcaccata ctcgtccagg
     2221 accagctggc gggcagtttc aagagcaggg aagccatgga taaggcccgg gcggagcagg
     2281 acttcaacct ttcccagagg gcggcgtccg taatactcct gaagatcaaa taagcggcgg
     2341 tgaaagggtg acggtcatga ccagaaaggt tcttctcggg tccctgttgt tgctgttcgt
     2401 tgttgccgtc gccgcggcgg aagggccgca aggccttaag atcgtctccg tcaagcccgt
     2461 gaagagcgga atatacgcga tcgcatcggt ggaattcccg aaggcctgga ccgaccccgg
     2521 gttcaccgtc gaagtgaacg gaaaacctgt caggatgagg caggtggggg gcggtttctc
     2581 cggggaccgc aacgtggcgg acctgatgtt catgccggga aggggagcga gacaatccgt
     2641 tgcggtgaag tgtttggtgg gaggaagaaa gaccgaggcc aagaccgggt tcgattggac
     2701 ggccacgcct tttgtcgccg tgccggggca cacggggaac cgggaagtga tcacggcgaa
     2761 ggagaaactg accatcgccg cggtcaatgt cacggggatg aggatattct tcaacggcaa
     2821 ggaggtccac gcgagatctt cgatgagagg cgatatcgag accttcacct tcgaacccgc
     2881 ctggaaaaag ggaaggaacg tgcttgccat aaacgctgac aaatgggact caggcatgat
     2941 cgcgaggaac ttcacctttt ttgacctcgg ggagggcggg actctatccc cagggcagag
     3001 cgccctcgtg caccttgggc gggaaggcac gaagagcggc cccttctatg atgtgaaggt
     3061 cgaaggcgat tccattgctc ctcctcaggg caccacggtc cgccgcgaca tcattgacga
     3121 ggacggatgg cttgtttccg agacatgggt tgcggtggag ttgagggccc ggaaggaggg
     3181 aaccgcgaag gtgcgtgttt tcgtcaagcc ccactttctt cggaacatgg aactggaaag
     3241 agagtttttg atcaccgtcg gggaagggtg aaaagaaggc gttctgccct tgtgcgtggt
     3301 tgacacgcga cagtcgcatt actatagtac gccagtgata accggtgaac gaagcgacaa
     3361 aagaagggga ggaaccatgg cgatgatcga tgaaaagaag attagggcct atcactccag
     3421 gagcgataac gcccttgttt gtccgttgtg cgcgacggat gaggagcgag agtccgcgga
     3481 gccgggagat cttgtcacgg aggacgtgat ccatgacgac aatcccatgt actgcgcgag
     3541 atgcaagaag aagatcgaga aacagggctc ctgacgcggg ggcgggatcg gggcctatcc
     3601 cctcctttgc ctcaggttcc cgaatctctt tgcccggacg ttgataagcc cgtccttctt
     3661 ctcgacgaca ccctccacga gcagaaaagg ctcgaggcgt atggtggtcc ggtgcatcct
     3721 gtagacctcc ggccgtacca cgacgttgat gagtccctcc tcgtcctcga gggtcatgaa
     3781 tgagaaccct cccttcgcgg tggggggcat ctggagacat accatgtacc cggcggtgag
     3841 cacggtcgaa cccggcgggc aacggggaag aactccgctc gtaaccgctc ccaggctgtc
     3901 gagggccttt cgatagagtt tcattatatg gtgcgaagcg gaaaagccct ggaaggcata
     3961 atcggttatc gtctcttctg ctgtgctcat ctccggtatg gggaggtttt cctgccggtc
     4021 ttcgcgcgcg agcgaagcga atcccgctgg tcccattgcg cgtagcgtcc ccgcctccca
     4081 cagaagctgt ctccttgacc gtttgatgtc gctgaaggcc ccgacggcga cgaggttgcg
     4141 cactgaccga tcgtcgagtc ggaccctgtc ggcaaagtcc tcgagcgatg tgaagatgtc
     4201 ctcccgccgt gccttgatga tacggtcgcc cttgtctttg ccgatgcctt tcacgtacct
     4261 gaaaccgatc ctcacggacc cctcctcgat ggagcagttc cacatgctcc tgttgatgtc
     4321 cacgggaagg atggggacgt tgtgccgttt cgcgtccccc gttatcacct cgggggcgta
     4381 aaatcccatg ggttgattgt tgagaagggc gcagtagaat tcggctggat agtatcgttt
     4441 caggtatgcc gaacgatagc acaggatggc aaagctggcg gcgtggctct tgcagaaacc
     4501 gaaatcggca aaaccgctta tatgatcgaa gatctttgtc gctagaccgc gcgctatccc
     4561 ctgttgttcc gccccctcga cgaaacggcc atgccacttc gcgagctcat cgcgggcgtt
     4621 cttccggccc atagccttgc gcagcatgtt ggcctcaccc gcggagaatc cggctatggc
     4681 cattgccacc tggagtatct gttcctggac gaggatcacc ccgagcgttt cgtcaaggat
     4741 gggggccagc ttcgggtgga gatggacgac gggttccttc ccctgccggc gccggatgaa
     4801 ggggtgcacc atgttgccct gcaggggccc gggtctcacg atggccactt ccatggtgag
     4861 gtcctcgaag gaccggggtc tcaccttggg aagggtttgc atctgggccc ggctttccac
     4921 ctggaagacg ccgaccgtat cggcatcgcc gatcattcta tagacctcgg gatcgtccgg
     4981 ggaaatgctg tcgaggttga ggtcgaggcc cttctgttcg cgtatgagtt ccttcgcgtc
     5041 atcgataagg gagagcatcc tcaggccgag aaggtcgaac ttgatgaggc ccgcgtcatc
     5101 gacggagtcc ttgtcccact ggcagacgat ccgcccttcc gcgcgggcgt gctcctgggg
     5161 gacgatctca tatatgggcc tgggggtgag gaccatgccg cccacgtgaa tggacagatg
     5221 ccgtggaaag tcggctattt tcgtgcagag gtctccgaaa tgctcccagg cgccggaatc
     5281 gaggtaggac gcgaattcgg ggacctcctt cagggaatcg atggcggcgc ggcctccata
     5341 ggcggagatg gtctttgcca tcctgtcgag gatgtgcgcg ggtagtccca ggacctttcc
     5401 cacctc
//
LOCUS       DAOQTR010000075         5302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17126_length_5302_cov_4.073947, whole genome shotgun sequence.
ACCESSION   DAOQTR010000075 DAOQTR010000000
VERSION     DAOQTR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17126_length_5302_cov_4.073947"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKN85_02830"
     CDS             <1..416
                     /locus_tag="PKN85_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNT43365.1"
                     /translation="GNYFVAFIFFLVGLHLVGVIPMPWSGPGNINMKRRGLLAAFILG
                     VVFGVALGPCTFAYMAPMLSITFQLSATKIWYGVLLLLAYAVGHCSIIVLAGTFTEAV
                     QNYLNWNERSMGARIVKIVCGVLVICGGIYLVYTA"
     gene            543..998
                     /locus_tag="PKN85_02835"
     CDS             543..998
                     /locus_tag="PKN85_02835"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNT43366.1"
                     /translation="MEKTRILFVCVHNSARSQMAEAFLNRLAGDRFHAESAGMDPGVL
                     NPIAVKVMKEEGIDISGNKTKSVFEKYRGGELYSYVVTVCDEASAEACPIFPGLRTHT
                     LHWSFEDPGSFTGSAEERLEKTRRVRDAIKRKVLQFMAEMGPDKGEGGS"
     gene            998..1345
                     /locus_tag="PKN85_02840"
     CDS             998..1345
                     /locus_tag="PKN85_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNT43367.1"
                     /translation="MKKVVLFAFNGEPMCFVHVLLNAVNMKTAGYDVKVVIEGAATKL
                     VPELVEEGNPLAVLYRNAREKGLIEGVCRACANKMGSLKAAEEQGLRLLDDMSGHPGM
                     ARYINNGYEVITF"
     gene            complement(1373..1603)
                     /locus_tag="PKN85_02845"
     CDS             complement(1373..1603)
                     /locus_tag="PKN85_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT43368.1"
                     /translation="MPIYEYKCEDCGAVSEFIVFSADEEVTCRSCSGTKLTKLMSAHN
                     TTSSSGSSSMPGFGGCCGSPGSCGSPGGCCSG"
     gene            complement(1613..1990)
                     /locus_tag="PKN85_02850"
     CDS             complement(1613..1990)
                     /locus_tag="PKN85_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT43369.1"
                     /translation="MKICFAVLKDEGVESTVYNHFGSAPAFVVVNTEKNDVFTVVNRD
                     ADHIHGACNPMKAIGGTEVDAVVVGGIGAGALTGLNSRGIKVFKATGTTIKDNLALFS
                     ENKLPELTIDRTCAGHAGGCAHH"
     gene            complement(1987..2289)
                     /locus_tag="PKN85_02855"
     CDS             complement(1987..2289)
                     /locus_tag="PKN85_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNT43370.1"
                     /translation="MSRPRKCRCVCCKLECDYFKPRGTPRTELVEVCLRIDELEALRL
                     ADVEGLYQEEAARQMNISRATFGRIVEAAHRKVADAILHGKALRIESEEAATGGRI"
     gene            complement(2397..4418)
                     /locus_tag="PKN85_02860"
     CDS             complement(2397..4418)
                     /locus_tag="PKN85_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNT43371.1"
                     /translation="MVVEYLQAMVIVLGISALIVFVLGKLRISSVVGFLLAGILIGPY
                     AFNLVGDPHEIELLAEIGVVLLMFTIGLEFSLKNLFQLKSIVLGGGAMQVALTVAFVV
                     CIGYFFYTQPLEGALFDGFLVALSSTAIVFKILLDRAQMNSPHGRFSVGILIFQDLCV
                     VPFMLLIPVLAGNSGGGSIALTIAKAALVIALILFASRWFVPFIFKQVVGMKSRELFM
                     ISIITMCLGTALLTSSMGLSLALGAFLAGMVISESEYAAQAVSDVLPFKESFTGLFFI
                     SVGMLLDLRVLASNIVGVSAMVFVIVILKVTAITITGMVLGNSLKNSLMTGLHLFQIG
                     EFSFILALEGKKHGLITNDLYQAFLSASIITMIMTPFFIRSSSSIAEWIARKSPLRLL
                     EKRRGRAGSERYPESIRDHVIIIGFGLNGGNLARVLRSLDIRYVTLELNSRTVRAGKR
                     KGEPIYYGDGTSAEILHKIGVRHASVLVVAISDAPSTRRIVQLARSSNPHLYIIARTK
                     YVAEVEGLKHLGANDVIPEEFETSVEIFSKVLNRLQVPVNDIREHIDRIRQDNYLALR
                     VVKLPRRYLVEREDILKNIVIETFRIRQGSVADEKTLKDLNLRSKTGVTVIGIERAGE
                     IHRMPSPSLRLMADDVLLFIGSREDIDCAVDYLDSNENEPPQCSIREDT"
     gene            4611..4964
                     /locus_tag="PKN85_02865"
     CDS             4611..4964
                     /locus_tag="PKN85_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNT43372.1"
                     /translation="MVEKWIEEIKRSVDPGELGMMLVHNGVVRATSKEGKPVKGMRLS
                     YDRALLAAAVEEVKKRDGIADVRVWINEGDLKVGDDIMYVLVAGRFRTDVLPVLQELL
                     TKVKGEVVHEQEVLP"
     gene            4961..>5302
                     /locus_tag="PKN85_02870"
     CDS             4961..>5302
                     /locus_tag="PKN85_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HNT43373.1"
                     /translation="MRKKVESACADCDIPLRERICTSPGGKGSKGCPTVLKGELIEKA
                     RREYRKRKVHEFARQASIQEGECYIDRDRKPYRLHPAKPRILEICEFAKKMGYRKLGL
                     VFCGGLAREAAT"
BASE COUNT         1189 a         1384 c         1614 g         1115 t
ORIGIN      
        1 acggcaatta tttcgttgct ttcatcttct tcctcgtcgg gctccatctt gtcggcgtca
       61 tccccatgcc ctggtccggg ccgggcaata taaatatgaa gaggagaggc cttcttgccg
      121 ccttcatcct gggggtcgtg ttcggtgtgg cgctgggccc ctgcactttc gcttacatgg
      181 cgcccatgct gagtataacc tttcaactct cggcaacaaa gatatggtac ggagtccttc
      241 tcctccttgc ctacgcggtg ggtcattgct cgatcatcgt ccttgccggc accttcaccg
      301 aggctgtcca gaactacctc aactggaatg agcggtccat gggggcgagg atcgtgaaga
      361 tagtctgcgg ggtcctcgtc atatgtggcg ggatctacct ggtctacacc gcgtaagaca
      421 gacgccggaa acgctccggg catttccggg gttgaaaaga ggggcaggaa ggcttagtgt
      481 gaacgcatga cagcccttcg ttacgcgttc caggggagat cggtcaaaat ggaggtggag
      541 caatggagaa gacgaggatc ctttttgtat gtgtccataa cagtgcgaga agccagatgg
      601 ccgaggcgtt tctcaacagg ctggcgggag accggtttca cgcggaaagc gcggggatgg
      661 atcccggcgt gttgaacccg atcgccgtaa aggtgatgaa ggaagagggg atcgatatat
      721 ccggcaacaa gacgaaaagt gtgtttgaga agtacagggg gggagagttg tattcgtacg
      781 tggtcaccgt ctgcgacgag gcgagcgccg aggcctgccc gatcttcccg gggctcagga
      841 cccacacgct tcactggtcc ttcgaggacc ccgggtcgtt taccggctct gccgaggaga
      901 ggcttgagaa gacgcgtcgc gttcgcgatg ccataaagag aaaggtcctg cagttcatgg
      961 cagagatggg gcctgacaag ggtgagggag gctcgtgatg aagaaagtgg tcctgttcgc
     1021 cttcaacggt gaacccatgt gttttgtcca tgtgttgctc aatgccgtaa acatgaagac
     1081 cgcgggatat gatgtgaagg tggtcataga aggggcggcc acgaagcttg tccccgaact
     1141 ggtcgaagaa ggcaatccgc ttgccgtcct gtaccgtaac gccagggaga aggggctcat
     1201 cgagggagtt tgcagggcct gcgccaacaa gatggggtcc ctgaaggcag cggaggagca
     1261 gggattgcgt ctcctcgacg atatgtcggg tcatcctggc atggcccgtt atatcaacaa
     1321 cggatacgag gtcataacct tctgaccccg gtctcggcct tcggcgcggg cattaccccg
     1381 aacagcagcc gccggggctg ccgcaggagc cgggtgaacc gcaacatccc ccgaaaccgg
     1441 gcatcgaaga tgagccggaa gagcttgtcg tattgtgggc cgacatgagc ttcgtcagct
     1501 tcgtgccact gcatgaccgg caggtcactt cctcatcggc tgaaaagacg atgaattcgc
     1561 ttacggcccc gcaatcttca catttgtatt cgtagatcgg cataagctct ccttaatgat
     1621 gggcgcatcc accggcatga ccggcgcagg tgcgatcgat cgtcagttca ggcagtttgt
     1681 tctcgctgaa aagggcaaga ttgtctttta tcgtggtccc cgtggccttg aagaccttga
     1741 tgccccttga gttaagcccc gtaagagcac cggcgccgat gcctcccacg accacggcgt
     1801 ccacttctgt cccgccgatc gccttcatgg gattacaggc accatgaatg tggtcggcat
     1861 cgcgattgac cacggtgaaa acgtcgtttt tttccgtgtt cacaacgaca aaggccggtg
     1921 cagatccgaa atgattataa acggtgcttt caacgccttc gtctttcaag accgcaaagc
     1981 atatcttcat atccttcctc ctgttgctgc ttcttccgat tctatcctca gggccttgcc
     2041 gtgaaggatg gcgtcggcga ctttccggtg tgccgcctcc actatcctgc cgaaggttgc
     2101 ccgggagatg ttcatctgcc tcgccgcttc ttcctgatag agcccttcga catccgcgag
     2161 gcggagggcc tccagttcgt caattctcag gcagacctcg acaagctccg tgcggggtgt
     2221 gccgcggggt ttgaaatagt cacactccaa cttgcagcac acgcacctgc atttcctcgg
     2281 ccgagacatg gatgtataat aagcatatgc tcaaaacaag tcaagggggt acaaaccgtt
     2341 acaggtttca ggtgcaaggc aaacgaaata ccatcggtcg acggaaagga agttttttaa
     2401 gtatcctctc ttattgagca ttgcgggggc tcgttctcat tggaatcgag gtaatcgaca
     2461 gcgcagtcga tgtcttcgcg gctgccgatg aaaagcagga catcgtcggc cataagacgc
     2521 agcgaaggag agggcatcct gtggatctcg ccggcacgtt cgatgccgat cacagttacg
     2581 cctgtctttg accggaggtt gaggtctttg agggttttct cgtcggctac cgaaccctgt
     2641 cgtatccgga acgtctcgat cacgatgttc ttgaggatat cttcgcgttc gacgaggtat
     2701 cttctcggga gtttcacaac cctcaaggca aggtaattgt cctgccgtat cctgtcgatg
     2761 tgttcccgga tatcgttgac ggggacctgc agccggttca gcaccttgga gaagatctcc
     2821 acggaggttt cgaattcctc gggtatgaca tcgttggctc caagatgctt cagtccttcc
     2881 acctcagcca cgtacttcgt gcgggcaatg atgtacaggt gagggttcga cgaccgtgcc
     2941 agctgcacta tcctcctcgt ggacggggcg tcgctgattg ctaccacgag gacacttgcg
     3001 tgccgaaccc ctatcttgtg gaggatctcc gcgctggttc cgtcgccgta gtagatgggc
     3061 tcacccttcc ttttccctgc ccggacggtc ctgctgttga gctcgagagt cacatatctg
     3121 atatccaggg aacggaggac acgcgcgaga ttaccgccgt tgagaccgaa gccgattatg
     3181 atcacgtggt cccttatgct ctcgggatag cgttcggagc ctgctctgcc tcttcttttt
     3241 tccaggaggc gaagagggct tttcctcgcg atccactctg cgatggacga cgagcttctt
     3301 atgaaaaaag gcgtcatgat catggtgatg atcgaagccg acaggaaggc ctggtaaaga
     3361 tcgttggtga tgaggccgtg cttttttcct tccagggcga ggatgaacga gaattcgccg
     3421 atctgaaaga gatggaggcc cgtcatgaga gagttcttga gtgagttgcc gaggaccatg
     3481 ccggtaatgg ttatggcggt taccttgagt atcactatca caaagaccat cgccgaaacg
     3541 ccgacgatgt tgcttgcaag caccctgaga tcgaggagca tgccgacgga gatgaagaag
     3601 agccccgtga agctctcctt gaagggaaga acatcggaaa cggcctgagc cgcatactcc
     3661 gattccgaga tgaccatgcc cgcaaggaag gcgccaaggg caagggacag gcccatggac
     3721 gaggtgagca gcgccgtacc gaggcacatc gtgatgatgg atatcatgaa gagctcccgg
     3781 cttttcattc ccacaacctg tttgaatatg aacggcacga accaccggga ggcaaagagg
     3841 atgagagcta tgaccagtgc ggcctttgca atggtgaggg ctatgctgcc gccgcccgaa
     3901 ttgccggcaa gaacggggat gaggagcatg aagggcacaa cgcagaggtc ctggaagatg
     3961 aggatgccca cggaaaagcg accgtggggg ctgttcatct gagcgcgatc gaggaggatc
     4021 ttgaagacga tcgccgtgct gctgagagcc acgagaaaac catcgaacag cgctccctca
     4081 agcggctggg tgtagaagaa ataacctatg cagacgacaa aggcgacggt aagcgcgacc
     4141 tgcatggctc cgcccccaag aacgatggac ttcagctgga agaggttctt gagggaaaac
     4201 tcgaggccga tggtgaacat gagcaggacg accccgatct cggcgaggag ttctatttcg
     4261 tgagggtcgc cgacgaggtt gaaggcgtac ggtccgatga ggataccggc aaggaggaat
     4321 cccaccacgg atgatatccg gagcttcccg agaacaaaca ctatgagggc gcttatcccg
     4381 aggacgatca ccatcgcctg aagatattca actaccatcc gttcctttct atccgagcat
     4441 gcaatcgcat atgccgggcc gcgtcacgac gcctggcgat ccttatataa tacgccgatt
     4501 tgcccggaaa accaaggggt tctctcgaaa acggccggcg ggaatccggg acggaaaccg
     4561 cttcttgatt ttccttcccg tggatggtag aaaccggtag gaggaagacg atggttgaaa
     4621 aatggatcga agagattaag agatcggtgg acccgggtga actcgggatg atgctcgtgc
     4681 acaatggggt cgtcagagcg acctcgaagg aagggaaacc ggtgaagggc atgcggcttt
     4741 cctatgacag ggcgctcctc gccgccgcgg tggaagaggt gaagaaacgt gatggaatag
     4801 cggacgtgag ggtctggatc aacgaaggcg acctgaaggt cggagacgac atcatgtacg
     4861 ttctcgtggc cgggcgcttc aggaccgatg tgctccccgt tcttcaggag cttctcacga
     4921 aggtcaaggg tgaggtcgtt cacgagcagg aggtgctgcc gtgaggaaga aagtcgaaag
     4981 cgcatgcgct gactgcgaca ttcctcttcg ggagcggatc tgtacgagcc ccgggggaaa
     5041 gggttccaag gggtgtccca cggtgctcaa gggagaactc atcgagaagg cacgccggga
     5101 gtacaggaaa cggaaggtcc acgaatttgc ccgccaggca tccatccagg aaggggagtg
     5161 ttacatcgac cgcgacagga agccctaccg gctgcacccc gcgaaaccta gaatactgga
     5221 gatctgcgag ttcgcgaaga agatggggta caggaagctg ggccttgttt tctgcggagg
     5281 attggcaagg gaagccgcga cg
//
LOCUS       DAOQTR010000076         5266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17275_length_5266_cov_2.793130, whole genome shotgun sequence.
ACCESSION   DAOQTR010000076 DAOQTR010000000
VERSION     DAOQTR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17275_length_5266_cov_2.793130"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKN85_02875"
     CDS             complement(<1..337)
                     /locus_tag="PKN85_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43374.1"
                     /translation="MKTTDRPTGVIFFPAFDWAISPTHPEREERLLYTRDQIFEEGIM
                     DLPEIREYHPRLASAKDVSRVHFCVPDVESRVTEAHMVSAGCAMVMADAFMQKEVKNA
                     FAIIRPPGHH"
     gene            complement(338..2014)
                     /locus_tag="PKN85_02880"
     CDS             complement(338..2014)
                     /locus_tag="PKN85_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNT43375.1"
                     /translation="MLIGIDVGGTYTDGILFSEGSVLLSVKHPTDENDLEGTLLSVLD
                     ELLARGDAKDVQRIVLSTTLVTNLLATGRGERTALLLLPGSGLPFSAYRISPDTYFLK
                     GGIDFRGRQIEAPDAKEVEEALRKVHDSGIDRVAVAGKFSNRNDAHERMVEDRARSLY
                     PHMDVCTSNEIAGRLNFPRRAVTCYYTAMTMREWNRFADGIEAAIRARAPRSEVHILK
                     ADGGTTTLEASRRRPCETVFSGPAASTMGTMALSRESLNAVSVDIGGTTSDISLLIEG
                     SPLYASKGALVGGHLTHINSFAVRSIPLGGDSRVYEESGELRVGPMRMGPAACLGGGE
                     PTVTDAFNLLNGLGLGDTGASRGKLEAVAGRLGTSLEELCQKVADEVVDRLEWNIEEM
                     FGLWEDEPAYKVWEVVHRRKFELHRVIGIGAASPAIIPRLAAKMNVEHFLHRYSPVAN
                     ALGAAVARPTLAVEVHVDTARRTYTGSPGGLRGTVEKRSFQLEDARELALNCLREASR
                     ERGLADYADEAHVYLEEQFNMIRGWEQEGKLFDVGIQIAPGFIHEYKGVA"
     gene            complement(2051..2986)
                     /locus_tag="PKN85_02885"
     CDS             complement(2051..2986)
                     /locus_tag="PKN85_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43376.1"
                     /translation="MARKIKEMSGIEEAIVGLIGARGPLTGFEIQKEIRTDSLLLWRA
                     CRTSGRLEVRSVGTRYMRLDRHVDGFARLSPSILREFLTYCVIGLPDDSSALDDRCLA
                     VGDRIREISRLKFDRALQMVLDVMEEYEDAAEMAGRMCFIIAGDIVYNMAHDVPRPER
                     STGKLVKGSDIDLVVVTDDDVADGPIGRLDELIYRRKYRMLIDPAVNEEVDYKIKRLM
                     RVREQAAFDDFKRMVAVKILDEGVFLHGSEEIFRAVKDIIRERGLDGRLAELEARAHV
                     FRSRAEMSILDGSLNGEKIKETHLFYSAEEFEEFE"
     gene            complement(3008..4357)
                     /gene="glmM"
                     /locus_tag="PKN85_02890"
     CDS             complement(3008..4357)
                     /gene="glmM"
                     /locus_tag="PKN85_02890"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNT43377.1"
                     /translation="MGKLFGTDGVRGEANRYPMNAEIAFAIGQAVVYLLKKTHDRPRI
                     VIGKDTRISGYMLEGALESGITSMGGNPYLVGVLPTPGIAFIAQSMRADAGIVISASH
                     NPYQDNGIKIFNGSGFKLSDEQEEAIEELMMSNTLHGLVPPVREMGQAFRLEDAHGRY
                     IVFVKNSFPRDLSIEGMKIVLDTANGATYKVAPDTFWELGADIEVIHNTPNGININDR
                     CGSQHTEDLRKRVVESGAAVGLAFDGDGDRLIAVDEKGREMTGDQILLICARMLKEQG
                     KLKNDLLVSTVMSNLGLRVACRKYGFRYHASKVGDRYVLEEMMRLGGVVGGEDSGHMI
                     FLDHHTAGDGIITALQLVAAMVRTGKPLSELAKWMDIYPQKLINVDVRTKPDIATIPQ
                     VMAVIDGVEKELGDEGRVLVRYSGTQNMCRVMVEGPSDAVTEKHCTEIADVIRAHLG"
     gene            complement(4392..>5266)
                     /locus_tag="PKN85_02895"
     CDS             complement(4392..>5266)
                     /locus_tag="PKN85_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43378.1"
                     /translation="QTILFPFVTLGSLINFCDCLMAGGTSRKNHSEVGSSYIHFNFTP
                     DGDKTTASLIGDVPRGVMLNQPPIFLGGQGGLVGPLRMGYGNVVAANSVLRSDFPEDN
                     KLIVGKTHAARTIDVRPGTYPNLHRIVGNNIIYIANLMALEQWYLHVRRPFLEGHEFG
                     SLIFSGLMDTLALAKQERLGRLRALSEKARRPEHGARSPKDGDGRNEFYERFAEIKAV
                     FTSVPGDDLAARSREVFLSLAGSLGGGGDYIEAIRGLDAGTAAKGTAWLQEVVDILCG
                     RVQELLPSLKMFGR"
BASE COUNT          893 a         1735 c         1541 g         1097 t
ORIGIN      
        1 catggtggcc gggcgggcgg atgatggcga aggcgttctt cacttccttc tgcatgaagg
       61 catcggccat caccatggcg catcccgccg ataccatgtg cgcctcggtc acgcggctct
      121 cgacgtcggg gacgcagaag tggacgcgcg agacgtcctt tgcagacgcg agccggggat
      181 ggtattccct tatctcgggc aggtccatga tgccttcctc gaagatctgg tcgcgcgtgt
      241 agagaagcct ttcctccctc tcgggatggg tggggcttat ggcccagtca aaggcgggaa
      301 aaaatatgac ccccgtgggt ctgtccgtcg ttttcatcta tgccaccccc ttgtattcat
      361 gaatgaatcc gggggcgatc tgtatcccca cgtcaaagag cttgccttct tgctcccagc
      421 cgcggatcat attgaactgc tcctccagat acacatgcgc ctcgtccgca taatcggcaa
      481 gcccccgttc gcggctcgcc tccctgagac aattcagcgc cagttccctt gcgtcttcga
      541 gctgaaagct gcgtttctcg acggtacccc tcaaccctcc cggcgaaccc gtgtaggtcc
      601 gccgtgccgt gtcgacgtgg acctccaccg cgagtgtcgg ccgtgccacg gccgcgccga
      661 gggcgttggc gacgggcgag taccggtgca ggaagtgctc gacgttcatc ttcgccgcca
      721 gccgggggat gatcgccggg gacgccgcgc ctatcccgat cacacggtgg agctcgaact
      781 tcctcctgtg aaccacctcc cagaccttgt aggcgggctc gtcctcccag aggccgaaca
      841 tctcctcgat gttccactcc agcctgtcga ccacctcatc ggcaaccttt tggcacagct
      901 cctcgaggga cgtgccgaga cgcccggcga cggcctcgag cttcccccgg gaggcaccgg
      961 tgtctccaag gccgaggccg ttcaagaggt tgaaggcgtc cgtcaccgtc ggctccccgc
     1021 cccccaggca tgcagcgggg cccatccgca tggggcccac gcgcagctct cccgattcct
     1081 cgtacacgcg gctgtcgccg ccgagcggta tggaccttac agcgaaggaa ttgatgtgcg
     1141 tgaggtggcc gccgacgagt gcgcccttcg aggcatacag aggcgaccct tcaatgagga
     1201 gactgatgtc ggaggtggtc ccgccgatgt ccacggacac ggcattgagg gattcgcggc
     1261 tcaaggccat ggtgcccatg gtgctcgccg ccggccccga gaacaccgtc tcgcagggcc
     1321 gcctccggct ggcctcgagg gtggtggtgc cgccgtccgc cttcaggata tgcacctcgc
     1381 ttcggggggc gcgcgcccgt attgcggcct cgatgccgtc ggcgaagcgg ttccattccc
     1441 tcatggtcat cgccgtgtag tagcaggtca cggcgcgccg gggaaagttt agccttcccg
     1501 cgatctcatt gctcgtgcac acatccatgt gcgggtacag ggaccgggcg cggtcctcga
     1561 ccatcctttc atgggcgtcg ttgcggttgg aaaacttccc cgcgacggcc accctgtcga
     1621 tgcctgaatc atggaccttc ctcagggcct cctccacctc tttcgcgtcg ggagcctcga
     1681 tctgcctgcc gcggaagtct atgccccctt tgaggaaata ggtgtcgggg cttatcctgt
     1741 aggctgaaaa gggcagcccg ctgccgggaa ggaggaggag cgccgtgcgc tccccccgtc
     1801 ccgtggcgag gagattggtg acgagggtgg tggagaggac tatcctttgc acgtccttcg
     1861 catccccgcg cgcgaggagc tcgtccagca ccgacagaag ggtgccctcg agatcgttct
     1921 cgtccgtagg gtgcttgacg ctcaaaagga ccgacccttc cgaaaaaagg atcccgtccg
     1981 tgtatgtgcc gcccacatcg ataccgatca gcatgttgtt cacctcttat ctttccccgg
     2041 ccgtccgggg tcattcaaat tcttcgaact cctccgcgga gtagaaaagg tgggtctcct
     2101 ttatcttttc cccgttgagg ctgccgtcga gaattgacat ctcggccctg gacctgaaca
     2161 cgtgtgcccg ggcttccagt tcggcgaggc ggccgtcgag gcccctctca cgaatgatgt
     2221 ccttgacggc gcggaagatc tcctcgctcc cgtggagaaa aactccctcg tcgaggatct
     2281 tgacggcgac catgcgcttg aagtcgtcga aggccgcctg ctccctgaca cgcatgagcc
     2341 tctttatctt gtagtccact tcctcgttca cggcggggtc gatgagcatc ctgtactttc
     2401 tgcggtatat cagctcatcg agcctgccta tggggccgtc cgccacgtcg tcatcggtaa
     2461 cgaccacaag gtcgatgtcg gaccccttga cgagcttacc cgtgctgcgc tccggacggg
     2521 gcacatcgtg ggccatgttg tacacgatgt ccccggcgat gatgaaacac atccggccag
     2581 ccatctccgc ggcgtcttcg tattcctcca tcacatcgag gaccatctgg agggcacggt
     2641 cgaacttgag cctgcttatc tcccggatcc ggtcgcccac cgcgaggcac ctgtcgtcga
     2701 gggcggaact gtcatccggc aggccgatga cgcagtaggt gaggaactcc cggaggatcg
     2761 acggggaaag gcgggcaaaa ccgtcgacat ggcgatcgag cctcatgtat cgcgtgccca
     2821 cggagcggac ctccagtctg ccggaggtcc tgcaagccct ccacaggagc aggctgtccg
     2881 tccgtatctc cttctggatc tcaaaacccg tcagggggcc tctcgcgccg atgagaccca
     2941 caattgcttc ttcaataccg ctcatttcct tgatcttccg cgccacgcgc gggaagctcc
     3001 cgcttctcta gccgagatgg gccctgatca catccgcgat ctcggtacag tgcttctccg
     3061 tgaccgcgtc tgacggccct tcgaccatca ccctgcacat gttctgggta ccggaatacc
     3121 gcaccaggac acgcccctcg tcgccgagtt ccttttcgac cccatctatc accgccatca
     3181 cctgaggtat ggtggcgata tcgggcttgg tcctgacatc gacattgatg agcttctggg
     3241 gatagatgtc catccatttc gccagctccg acaggggctt gcccgttctg accatggcgg
     3301 cgacgagctg gagggcggtg atgatgccgt cgcccgcggt gtgatggtcc agaaagatca
     3361 tgtgccccga atcctcgcca cccaccacgc cgccgaggcg catcatctcc tcgaggacgt
     3421 agcggtcgcc gacctttgac gcgtggtacc tgaagccgta tttcctgcag gcgaccctta
     3481 gcccaaggtt actcatcacg gtgctcacga gaaggtcgtt cttcagttta ccctgctcct
     3541 tgagcatcct cgcgcagatg agaaggatct ggtcgcccgt catctcccgc cctttctcat
     3601 cgacggcgat gaggcggtcg ccgtccccgt cgaaggcgag cccgacagcc gcgccgcttt
     3661 ccacgaccct ctttctgagg tcctccgtat gctgcgaacc gcacctgtcg ttgatgttga
     3721 tgccgttcgg cgtattgtgg atgacctcga tgtcggcgcc cagttcccag aatgtatcgg
     3781 gggcaacctt gtacgtggcg ccgttggcgg tatcgaggac gatcttcatc ccctcgatgg
     3841 agaggtcccg gggaaagctg ttcttcacga aaacgatgta tctgccgtgc gcgtcctcga
     3901 ggcggaaggc ctgccccatt tcccggacgg ggggcacgag gccgtgaagc gtattgctca
     3961 tcatcagctc ctcgatggcc tcctcctgtt cgtcggagag cttgaagccg ctgccgttga
     4021 atatctttat tccgttgtcc tgataggggt tgtgggaggc ggagatcacg atccccgcgt
     4081 cggcgcgcat gctctgcgct atgaaggcta tccccggcgt cggcaggaca ccgacgagat
     4141 aggggttgcc ccccatggag gttatccccg actccagcgc tccctcgagc atgtacccgg
     4201 agatgcgcgt gtccttcccg atcactatcc ggggacgatc gtgcgtcttc ttcaggagat
     4261 agaccaccgc ctgcccgatg gcaaaggcta tctccgcgtt catcggatac cggttcgctt
     4321 ccccccggac gccgtcggta ccaaaaagtt tacccatgat catccctcac tcgtctcacg
     4381 ccttctccat gtcatctgcc gaacatcttc agggacggca ggagttcctg cacccttccg
     4441 cagaggatat cgacgacctc ctgcagccat gccgtgccct tcgccgcggt cccggcgtca
     4501 agtccccgta tggcctctat gtagtccccg ccgcctccaa ggctgcccgc aagggagaga
     4561 aaaacctcgc gggaccgcgc cgcgaggtca tcccccggca cggatgtgaa gaccgccttt
     4621 atttccgcga acctttcgta gaactcgttc ctgccgtcgc catcctttgg tgaccgtgct
     4681 ccgtgctccg gccttcgggc cttttccgag agcgcccgaa gcctcccgag ccgctcctgt
     4741 ttcgccaggg ccagcgtgtc catgagcccc gagaagatga gactcccgaa ctcgtgccct
     4801 tcgaggaagg ggcggcgcac gtggaggtac cactgttcca gcgccatcag gttggcgata
     4861 tatataatgt tgttccccac gatgcggtgg aggttcggat aggtccccgg cctgacatcg
     4921 atcgtcctcg ccgcatgcgt ctttcccacg atgagcttgt tgtcttccgg gaaatcgctc
     4981 ctcagcaccg aattggcggc caccacgttc ccgtacccca tcctgagggg accgacaaga
     5041 cccccctgac cgcctaagaa gatggggggc tggttgagca tgaccccgcg ggggacgtcc
     5101 ccgatgaggg atgccgtcgt cttgtcgccg tcgggtgtga aattgaagtg gatgtaagag
     5161 cttcccacct cgctgtggtt cttacggctc gtccctcccg ccatcaggca gtcgcagaag
     5221 ttgatgaggc tccccagggt cacgaaggga aagagtatgg tctgct
//
LOCUS       DAOQTR010000077         5214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17528_length_5214_cov_3.552045, whole genome shotgun sequence.
ACCESSION   DAOQTR010000077 DAOQTR010000000
VERSION     DAOQTR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17528_length_5214_cov_3.552045"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..539)
                     /gene="smpB"
                     /locus_tag="PKN85_02900"
     CDS             complement(93..539)
                     /gene="smpB"
                     /locus_tag="PKN85_02900"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNT43379.1"
                     /translation="MKVICTNRKAYHEYHIEEKFETGIVLTGTEVKSLREGRANLKES
                     YAKVKDGEVFVVNAHISPYSCGNIYNHEPKRTRKLLMHKREIMRLIGKVKERGYTLIP
                     LSLYFDKRNRAKMELALAKGKTLYDKREGIRRKDEKRLMEREERMR"
     gene            complement(759..2885)
                     /locus_tag="PKN85_02905"
     CDS             complement(759..2885)
                     /locus_tag="PKN85_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43380.1"
                     /translation="MKLKKELSIIIGILVAFGALVLIESSLPNFARFFPVWENKLLVV
                     VLNINLLLILLLLFLITRIILKAYIEKKRGIWGSGLKKRLTSTLLFISIVPSFTLFIL
                     ATGFFYVSMDRWFSQQVEDTMESALELSRLHYEEVFQRHTRTGEQMAEEIGKKELLED
                     EKGLRKLLRQYERTHLLGYVAVYDPRSAAMVRVGGSAKSERTILSKLKLFTGKEVSQE
                     IIPDEQGEMLVVGMRLAPAGDSAPILFLGDTIRIQGKERMRQIAVTSKEFRESRVFKK
                     VLKYSFIIPLFLITILTIFVSLWVGIKMATEITVPIEKMKEGAAIIATGKFDINLEDR
                     GKDEIGTLVKAFNTMAKELKIAKDEIEDKSRSLEFVLNNVAAGIISTDKKGTILLANN
                     AARVILGVEREKLEGMSLREVFGNDFKALTKSFLREVRASKGESITKDLRLNLKKDIT
                     YLRASLTALKDERRRVAGFIIAFDDITHVVRAEKLATWQEVARKLTHEIKNPLTPIVL
                     SAERIRRKLMARFTGPDKGVLDETTSVIIRSVADIKDLVNELTKLTHTSQARTLEDLN
                     GVVDETLDLYRNLYHNISIGFEPGELPLVRVDRDGLKRAFINLITNAVKAIDGKAGTI
                     TVRTRYDRAKRAGIIEFTDSGKGVPDQDKEKVFDPYFTKDEGGTGLGLAIVHSIILEH
                     HGRIRVEDNVPEGAKFIIEIPITETA"
     gene            complement(3087..3803)
                     /locus_tag="PKN85_02910"
     CDS             complement(3087..3803)
                     /locus_tag="PKN85_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNT43381.1"
                     /translation="MKEFVLENRNDWEYYHVPGLTDAGILHGFFTRRSPASPLRGREA
                     EAFLDAFSLSDAVSLSQEHGTDIHVIANGDRPSKGDGILLYKRGIAGVINTADCLCVI
                     LVDPGFPMVSIVHAGWRGTLKKITSRAVGMMTERGAATQRMLALMGPSIRGCCYEVGR
                     EVTDAFLDAGFPGNILTSVNGSLHLDLEAANAHLVLEEGVTSIFDTGLCTCCSGDDFV
                     SYRRGARNARQMNFVAIRDR"
     gene            complement(3856..4815)
                     /locus_tag="PKN85_02915"
     CDS             complement(3856..4815)
                     /locus_tag="PKN85_02915"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNT43382.1"
                     /translation="MTDSFRIVCDVSDVRLDVFLSEKLSLTRTKVKEMIDGGHVSVDG
                     RTPKPAMRLKERMEIAGEVPRETALDVAPEEIALDILYEDDHFLAVNKPAGMVVHPSF
                     GHSSGTLVNAVLGYLERLGSRFKVQGSREQEPCSESDMAGVRPGIVHRLDKGTTGVIL
                     VARDERTQERLSVLFKDRKVRKTYRAVVEGTPGPDSWTVEGNIGRHPVERKKMAVLRS
                     GGRQAQTSFKVLERLEGFSYVEAYPTTGRTHQIRVHLNHAGYPIVGDETYGRRAKRSA
                     ARPLLHAWRIEFIHPATLKPVAIEAPVPADMIDFVEKHRGQLH"
     gene            complement(4805..>5214)
                     /locus_tag="PKN85_02920"
     CDS             complement(4805..>5214)
                     /locus_tag="PKN85_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43383.1"
                     /translation="AGLEATLLLCLDAGSAAGIPTIRMILEEKGAVGKRARRVASQLR
                     KRCPDAGVSVIDVDSEVGGGSFPDMVIPSVGVALESRTMTIAALEAKLRHLPLPVIAR
                     IEKERLILDLRTVLAEDEADLVAAVARAFENDR"
BASE COUNT          931 a         1628 c         1459 g         1196 t
ORIGIN      
        1 gcagttgtgt ttagttccgc ttgggtacgg gtaagcagta acccggcatg cctctctgac
       61 cctcacttcc ctgtcgaagc cagtcgcccc cgtcatcgca ttctttcctc acgttccatg
      121 agacgtttct cgtccttgcg cctgattccc tcccttttgt cataaagtgt cttccccttt
      181 gccagtgcca gttccatctt ggccctgttc ctcttgtcga agtaaaggga aagggggatc
      241 agcgtgtatc ccctctcctt gaccttgccg atgagccgca tgatctccct cttgtgcatg
      301 aggagcttgc gcgtcctctt cggctcgtgg ttgtatatgt tcccgcagga ataggggctt
      361 atgtgggcgt tcacgacgaa aacctcgccg tccttgacct tggcgtagct ttccttgagg
      421 ttcgccctgc cttccctgag tgacttcact tccgtgcccg taaggactat ccccgtctcg
      481 aacttctcct cgatatggta ctcgtgatag gccttcctgt tcgtgcagat aaccttcata
      541 ggcaataata taacacagaa aggcgggaaa agacaggtct gccgggctcc ggcccggcag
      601 accgcttgaa cagcttgaac agcttgaacc gtaggaaagc gggtaaggca tctacgcaag
      661 cagtgtcccc ggtcgatggt attgtgccat gccgccggga ctttccttcg tcttttaacg
      721 ttcaagcggt tcaagcggct gcaactgtct tttccggttc aagcagtctc tgttatgggt
      781 atctctatga tgaacttcgc gccttctggc acgttgtctt cgacccgtat cctgccgtgg
      841 tgctcgagga tgatggagtg gacgatcgcg agaccgaggc cggtgccgcc ttcgtccttc
      901 gtgaagtagg ggtcgaagac cttctccttg tcctggtcgg ggacaccctt gccgctgtcc
      961 gtaaattcga tgatgccggc gcgcttcgcc ctgtcgtacc gcgtgcggac cgtgatggtc
     1021 cccgctttgc cgtcgatggc cttgacggcg tttgtgatga ggttgatgaa ggcccgtttc
     1081 agcccgtcgc ggtccacacg cacgagaggg agttccccgg gctcgaaacc tatcgatatg
     1141 ttgtgataga ggttccggta gaggtccagg gtctcgtcaa cgacgccgtt caggtcctcc
     1201 agggtcctcg cctgggatgt gtgggtgagc ttggtaagct cgttgaccaa gtccttgata
     1261 tcagccacgg accggatgat gaccgaggtc gtctcgtcga gaacgccctt gtcgggccct
     1321 gtgaaccgcg ccatcagctt cctgcggatg cgttcggccg acagaacgat gggcgtgaga
     1381 gggttcttga tctcgtgggt gagcttgcgc gccacctcct gccatgtagc cagcttctcc
     1441 gcgcgcacca cgtgggtgat gtcgtcgaag gcgatgatga atcctgccac ccttctgcgc
     1501 tcgtccttaa gggccgtgag cgaagccctg aggtatgtta tgtccttctt gaggttgagc
     1561 cttaaatcct tcgtgatgct ctcgcccttc gaggccctga cctccctcag gaacgacttc
     1621 gtaagcgcct tgaaatcgtt gccgaagacc tctctcaggg acatcccctc cagcttttcc
     1681 ctctcgacgc cgagtatgac gcgcgcggcg ttgttcgcga gaaggatggt tcctttcttg
     1741 tccgtcgaga tgatgccggc ggccacattg ttgaggacga actccaggga gcggctcttg
     1801 tcctctatct cgtccttggc tatcttgagc tccttcgcca tggtgttgaa ggccttcacg
     1861 agcgtgccga tctcgtcctt tcccctatcc tcgagattga tgtcgaactt ccccgtggct
     1921 atgatggccg cgccttcctt catcttctct atggggacgg tgatctccgt ggccatcttg
     1981 atgcccaccc agagggagac gaagatggtg aggatggtga tgaggaaaag aggaatgatg
     2041 aaactgtact tgaggacctt cttgaacacg cgggattccc tgaactcctt gctcgtgacg
     2101 gcgatctgcc tcatgcgctc cttgccctgg atccttatcg tgtcaccgag aaagagtatg
     2161 ggagcgctgt cccccgccgg cgcgaggcgc atgcccacca cgagcatctc gccctgctcg
     2221 tcggggatga tctcctggga aacctccttc cccgtgaaga gcttcagttt cgaaaggatc
     2281 gtcctttcgg acttcgcgga gcccccgacc ctgaccatcg ccgccgaccg gggatcgtaa
     2341 acggccacgt agccgagcag gtgggtccgc tcgtactgcc tgaggagctt tctgaggcct
     2401 ttctcatcct cgagcagttc ctttttcccg atctcctccg ccatctgctc cccggtcctc
     2461 gtgtggcgct ggaagacctc ctcgtagtga agccgggaaa gctcgagggc gctttccatg
     2521 gtgtcctcga cctgctggct gaaccaccgg tccatgctca cataaaagaa acccgtcgcc
     2581 agaatgaata gggtgaaaga aggcacgatg gaaataaaga ggagcgtcga ggtcagcctc
     2641 tttttgaggc ccgaccccca gatcccccgt ttcttctcga tgtatgcctt gaggatgatg
     2701 cgggtgatga ggaacagaag cagaaggatg agcaggaggt tgatgttcag aacgacgacg
     2761 agaagcttgt tctcccacac cgggaagaac cgcgcaaagt tgggaagact cgactcgatc
     2821 aggacaagcg ccccgaaggc aacgaggata ccgatgatga tcgaaagctc ttttttaagt
     2881 ttcataagaa cgggtcttgg gctatgggtg ataggtaatc ggaaataccc tctcctcttt
     2941 acctcttacc ccattacctg caacccatca cccgtctctt ccttttctct tcccatcgcc
     3001 tatcacctat cgcccgcctc ttctttgtcc tttcccgctg cccataacct atcacccata
     3061 acccgtcttt tccctttctt ctcccatcac ctgtctctta tcgccacaaa gttcatctgc
     3121 cgggcgttgc gggcgcccct gcgataggaa acgaagtcat caccgctgca gcaggtgcac
     3181 aatcccgtgt cgaagatcga cgtgacgccc tcctcgagga cgaggtgagc gttggcagcc
     3241 tcgaggtcga ggtgcagcga accgttgacg gaggtcagga tgttcccggg gaaacccgcg
     3301 tcgaggaacg cgtccgtgac ctccctgccg acctcgtaac agcagccgcg gatcgagggg
     3361 cccatgaggg caagcatgcg ctgtgtcgcc gctccccgct ccgtcatcat cccgaccgcc
     3421 cgggaggtta tcttcttgag ggtgccgcgc cagccggcgt ggacgatgga caccatgggg
     3481 aagccggggt cgacgagaat gacgcacagg cagtccgcgg tgttgatcac ccctgcaatg
     3541 ccccgtttgt acagaaggat cccgtcgccc ttcgaaggcc tgtcgccatt agcgatcacg
     3601 tggatatcgg ttccgtgctc ctggctcagc gagacggcgt cgctgagcga aaaggcgtcg
     3661 aggaacgcct ccgcctcccg tcccctaagc ggggacgcag gcgacctccg ggtgaagaac
     3721 ccgtgcagga tacccgcatc cgtcaacccc gggacatggt agtattccca atcattcctg
     3781 ttctcgagta caaattcctt cacaacccca ccccatcctt acagaacacg tttcatgttg
     3841 tagtttacgg gttttctaat gcagttgtcc acgatgtttt tcgacgaaat cgatcatgtc
     3901 tgcgggaacg ggcgcctcga tggccacggg tttcaatgtc gcggggtgga tgaactctat
     3961 ccgccaggcg tggagaaggg gcctcgccgc ggacctcttc gccctcctgc cgtatgtctc
     4021 gtcgccgacg atgggatagc cggcgtggtt caggtggacc cgtatctggt gggtcctgcc
     4081 cgtcgtggga taggcctcga cgtaggaaaa accctccagc ctctccagga ccttgaagct
     4141 tgtctgcgcc tgccttccgc cggagcgcag cacggccatc ttctttcgct ccaccgggtg
     4201 gcgcccgata ttgccctcga ccgtccagga atcggggccg ggcgtcccct ccacgaccgc
     4261 ccgatacgtc ttccggacct tcctgtcctt gaagagcacc gacagtctct cctgcgtcct
     4321 ctcatcacgg gcgacgagaa tgacccccgt cgtccccttg tccagccgat gcacgatccc
     4381 cggcctcacc cccgccatgt cgctttcgct gcagggttcc tgctctcttg atccttgaac
     4441 cttgaacctt gaacccagtc tctcgaggta tcccagcacc gcgttcacca gcgtgcccga
     4501 gctgtggccg aaagacgggt ggacgaccat acccgccggt ttgttgacgg ccaggaagtg
     4561 atcgtcctcg taaaggatgt cgagggctat ctcctccggc gccacgtcga gggccgtctc
     4621 ccgcgggacc tctcccgcta tctccatgcg ctccttcagg cgcatggcgg gcttgggcgt
     4681 cctgccgtcg acgctcacgt ggccgccgtc gatcatctct tttaccttgg tccgtgtcag
     4741 ggaaagcttt tcggagagaa agacgtcgag ccgcacatcg cttacgtcac agacgatcct
     4801 gaaactatcg gtcattttcg aaggcgcggg ccaccgccgc gacgagatcg gcctcatctt
     4861 ccgcgaggac cgttctcagg tcgagtatga gcctttcctt ttcgatccgc gcgatgacgg
     4921 ggagagggag atgtctgagc ttggcctcca gggccgcgat ggtcatcgtc cgggactcga
     4981 gtgccacacc gacggaaggg atgaccatgt cgggaaagct cccgccgccc acttcggagt
     5041 cgacgtcgat gacggagacc ccggcatcgg ggcatctttt cctcaactgt gaggcgaccc
     5101 gcctcgccct cttcccgacg gcccccttct cctcgaggat catcctgata gtgggaatcc
     5161 cggccgccga accggcatcg aggcagagga gcaacgtcgc ctcaaggccg gcaa
//
LOCUS       DAOQTR010000078         5204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17580_length_5204_cov_3.985628, whole genome shotgun sequence.
ACCESSION   DAOQTR010000078 DAOQTR010000000
VERSION     DAOQTR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17580_length_5204_cov_3.985628"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKN85_02925"
     CDS             <1..299
                     /locus_tag="PKN85_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT43384.1"
                     /translation="RWPGNVRELANVMERAVLLCASRETILPEDLPQSMMEGRFADRE
                     ADRRELTSADELSLHHIEQEHIERVLTLAKGNKSKAARLLGISRRKLYLKTQEK"
     gene            621..1637
                     /locus_tag="PKN85_02930"
     CDS             621..1637
                     /locus_tag="PKN85_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT43385.1"
                     /translation="MKRCVSIVSVVLFLAVTFCLTSVPASAQVKLRFATFFPVSHPNA
                     AITAEWCKEVEKRTNGKVKVQHFPGATLTSPQSQYDSILTGVVDIANCVLGYTMGKFP
                     LSEILDYPLGYPSGAVATRLGNEFYKQFKPKEFDDVKVMYFHAQGPGILHTRKPVKNL
                     DDLKGMKIRTFGSNAKMMSMLGGSPVAMPMGDAYDALSKGVADGLLCGYEALKGWKLG
                     EVIKSTTENYGTAYTATFVIAMNKAKWNSIPPDAQKVIEEINKEFIEKQGKLWDKMDQ
                     DGKEFSVKRGNQVIKLSAEENQKWITKIEPLFAEYVKKMKEKNLPGDKALKFVREYIK
                     KNTK"
     gene            1707..2195
                     /locus_tag="PKN85_02935"
     CDS             1707..2195
                     /locus_tag="PKN85_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT43386.1"
                     /translation="MNSVVGAVRRFSGWMNGLSGIVLFLMMMLTVVDVALRIFWKPIT
                     GTYELVAMAGAVVVAFAIPQTSWDNAHIFVDFLLEKRSRGVKTAFGCFTKVLGVTLFV
                     MLGWYLLSKANHLFRSGDVSLTLGIPYYPVAYGLAFCAFMEALVLVLHIVMLFRNGEN
                     NG"
     gene            2188..3489
                     /locus_tag="PKN85_02940"
     CDS             2188..3489
                     /locus_tag="PKN85_02940"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNT43387.1"
                     /translation="MDEITIGVIGIFVLLALFLTGLELAFAMALIGVAGYAYIVGPAQ
                     AMNMLANDFYDSLESYGLTVVPLFVLMGQIAFNAGVAKRLYDSAHKFLGHIPGGLAMA
                     TVAGATIFKAICGSIVATSATFASVAVPEMDRFNYSKKLSTGIVATVGTLGVLIPPSV
                     TLILLGLITQVSIGKLFMAGLIPGLMISFFFALVIFGWARINPEIGPKSEKYSWPARM
                     KTLPDVMWPVLIFLIIIGGLMNGFFTPTEAGSIGTFAVLVLCILKGDIKFNGIRQSIR
                     EALRTSCMVLVIVASSTVLGHFIAVTNIPNGVAEWVVSLPVHRHIIMIFIFLIYLIGG
                     SFIDDLAFLILATPIFYPAITNMGYDPIWACIMLSLTVCVGSVIPPVAMCVFVVKNIT
                     KVPMGVIYKGVYPFLIALFLCVALLFVFPELTLYLPSVLMK"
     gene            3936..>5204
                     /locus_tag="PKN85_02945"
     CDS             3936..>5204
                     /locus_tag="PKN85_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNT43388.1"
                     /translation="MTPNHDFQTVRTLITRNAALYPQKMAMKEFETGRNCTFEDLLER
                     AKRIGSALHGMGVKKGDRVGIMSQNSYEYMELILSVTAAGYVFVPVNFRLAGREMAGV
                     LSDAGPVVLFVHEQYVKTAHEIKGEIPSVREYVHIGPAETRPDGWRGYEEMIQCAAPM
                     GWVESVEEGDLAMLMYTSGTTGAPKGVMQTQLNLYHCGRTCAFNNCIDTEDIGFTICP
                     MYHVTATTSFFGPFYRGATSILFQKFDAEALFEAAREERIVAGMLATPMVRMLLDVWP
                     SVKDRYDVSSMKKLWFAGAGIIPAVYKQFIDTFGCILGEHHGTTETTGVTANLSAKDI
                     AKELTDDNLRILESCGRDAYDCEVLIVDDNDNPVVPPGEGEMKVRGLGTSLGYWRKEE
                     QTKKAFRNGWFYTEDICHIDEKGYIYIIDRK"
BASE COUNT         1105 a         1512 c         1530 g         1057 t
ORIGIN      
        1 accgctggcc cggcaacgtc cgcgaactgg cgaacgtcat ggagcgggcc gtgcttctct
       61 gtgcctcccg ggagacgatc cttcccgaag acctccccca gagcatgatg gagggccgtt
      121 tcgcagaccg cgaagcggac cggcgggagc tcacctccgc cgatgagctg agcctccacc
      181 acatcgaaca ggaacacatc gaacgcgtcc tgaccctcgc caagggcaac aagagcaaag
      241 ccgcccgcct cctggggata agcaggagaa aactgtactt gaagacacag gagaaataaa
      301 gaccgcttga accgcttgag tcgcttgaac gttgaaagac ggagctggcg ctgaagggtc
      361 ctgacttttc agtcacgtcc cgaggtagcg gtaaggttcc tgtttctaac gttcaagcgg
      421 ttcaagcggt tcaagccggc tgcggccccc gggccgcagc catgtggtta aaagatgcac
      481 acccatgtat caattatgca cacccggctg aacggagttg agcgggagtc actgatttcg
      541 gggtcttacg ggggtttaag gggtggcatc gaaattgctt ttcaaaaatg tcagcaaatc
      601 caaaatcaag ggggtttctt atgaaacggt gtgtgagtat tgtatccgtt gtcctgttcc
      661 ttgcagtgac cttctgcctc acttcggtgc cggcgagcgc ccaggtgaaa cttcgtttcg
      721 ccaccttctt ccctgtgagc catcccaacg cggcaatcac ggcggagtgg tgcaaggagg
      781 tcgagaagag gacgaacgga aaggtgaagg tccagcattt cccgggtgcc accctgacgt
      841 cgccccagtc ccagtatgac agcatcctga ccggtgtcgt ggacatcgcg aactgtgtcc
      901 tcgggtacac gatggggaaa ttccccctct ccgagatcct cgactacccg ctgggttatc
      961 cgagcggcgc cgtcgcgacg aggcttggca acgagttcta taaacagttc aagcccaaag
     1021 aattcgacga cgtgaaggtc atgtacttcc acgcccaggg cccgggaata ctccacacga
     1081 gaaaacccgt gaagaacctc gatgatctga agggcatgaa gatcaggacc ttcggctcca
     1141 acgcgaagat gatgtccatg ctcggcggct cgccggttgc catgccgatg ggcgatgcct
     1201 atgacgccct ctccaagggc gttgccgacg gcctcctgtg cggttacgag gccctgaagg
     1261 gatggaagct cggcgaggtc atcaagtcca cgacggagaa ctacggcacc gcctacaccg
     1321 ccaccttcgt catcgccatg aacaaggcga aatggaacag cattcctccc gacgcccaga
     1381 aggtgatcga ggagatcaac aaggagttca tcgagaaaca gggcaagctc tgggacaaga
     1441 tggaccagga cgggaaggaa ttctccgtga aaaggggaaa ccaggtgatc aagctttccg
     1501 ccgaagagaa ccagaaatgg atcacgaaga tcgagcctct cttcgccgaa tacgtcaaga
     1561 agatgaaaga gaagaacctt ccgggcgaca aggcgctcaa gttcgttcgc gagtacatca
     1621 agaagaacac gaaatagccc ccataaggcc aggctgccgg gaagagtgaa cgggcccggc
     1681 agccggctcc ctaaggagga tgatcgatga acagcgtggt cggagccgtc aggaggttca
     1741 gcggctggat gaacgggttg agcggcatcg ttctgttcct gatgatgatg ctcacagtgg
     1801 tggacgttgc ccttcggatc ttctggaaac ccatcacggg gacctatgag ctcgttgcaa
     1861 tggcaggggc ggtcgtcgtg gcttttgcca taccgcagac ctcctgggac aacgcccaca
     1921 tattcgtgga ctttctgctc gagaaacgtt cccggggggt aaaaacggcc ttcggctgct
     1981 tcacgaaggt cctcggggtg acactcttcg tgatgctggg ctggtatctc ctctcgaagg
     2041 cgaaccatct tttccgaagc ggggatgtat cccttaccct gggaatacct tactatcccg
     2101 tagcctatgg actggcgttc tgtgccttca tggaggccct cgtccttgtc ctgcatatcg
     2161 tcatgctgtt caggaatggt gagaacaatg gatgaaatta ccataggggt catcggcatc
     2221 ttcgtccttc tggcactctt tcttaccggt ctggagctgg ccttcgccat ggccctcatc
     2281 ggtgtggcag ggtacgctta catagtcggg cccgcacagg caatgaacat gctggccaac
     2341 gacttctatg actctctcga gtcttacggt ctcacggtcg tgccactctt tgtcctcatg
     2401 gggcagatcg ccttcaacgc cggggtcgcc aagcggctct acgacagtgc ccacaagttc
     2461 ctgggacaca tccccggagg cctggcgatg gccaccgttg ccggagcgac gatcttcaag
     2521 gccatatgcg gctccatcgt ggcgacaagc gccaccttcg cgagcgtcgc cgtgcccgag
     2581 atggacaggt tcaattacag caagaagctc tcaacgggca tcgtggccac cgtcgggacc
     2641 ctgggcgtcc ttatcccgcc gagcgtgacg ctcatcctcc ttggcctcat cacgcaggtg
     2701 tccatcggaa agctcttcat ggcgggactc atcccgggtc tcatgatctc cttcttcttc
     2761 gccctcgtca tcttcgggtg ggcacgcatc aaccccgaga tagggccgaa gagcgaaaag
     2821 tactcctggc cggcccggat gaagaccctt cccgacgtga tgtggcctgt gctgatcttc
     2881 cttatcatca tcggcgggtt gatgaacggt ttcttcacgc ccaccgaggc ggggtccatc
     2941 ggaaccttcg cggtcctcgt cctgtgcatt ctgaagggcg atatcaaatt caacggcata
     3001 cggcagtcca tcagggaagc ccttcggacc tcctgcatgg tcctcgtcat cgtggcgtcc
     3061 tcgacggtgc tgggtcattt catcgccgtc acgaacatcc ccaacggggt ggcggagtgg
     3121 gtggtgagcc tgccggtcca tcgccacatc atcatgatct tcatcttcct catttacctt
     3181 atcggtggct ccttcatcga tgaccttgcg ttcctcatcc tcgcgacacc gatattttac
     3241 ccggcgatca cgaacatggg atacgatccg atctgggcgt gcatcatgct gtccctcacc
     3301 gtctgcgtcg gctccgtcat tcctcccgtc gccatgtgcg tcttcgtggt gaagaacatc
     3361 accaaggtcc ccatgggcgt tatctacaag ggcgtctacc ccttcctcat cgcgctgttc
     3421 ctctgcgtgg ccttgctttt cgtcttcccc gaactgacgc tgtatctgcc gtcggttctc
     3481 atgaaatagg ccgttcgtat acactccttc gatacccggg ccggatggag cgctgccatc
     3541 cggcccaaag tttctaaaga cggcttgaac cgcttgaatg gcttgaatgg cttgagggta
     3601 gaagagagaa gaacgggtgt gaaggggggt gtctgcaggg atgtctttat gtaatgaaat
     3661 tgaacaggtg tgcagaaaat gcacaggcgg gagatcgatg ggggtgccgg gttcccgggc
     3721 acgcgcgtcc cgctgttgtt cgtcgtttcc gtgacatcga cgtaataccc cgaaatcaat
     3781 tggatagcaa gggtccgccg ccacccgccc cgtctgctgt ctccaacctg cgacctgcga
     3841 cctgcgacct gcgaccgtct tttcagtggc atgctaattg ctcatgatga tgtgcgaatc
     3901 tcactctatt actgcacttc aggaggcgac tggaaatgac cccgaaccat gactttcaga
     3961 cggtaaggac gttgatcacc cgcaacgcag ctctgtatcc gcagaagatg gcgatgaagg
     4021 aattcgagac aggaaggaac tgtacctttg aggacctgct ggagagggcg aagaggatcg
     4081 ggagcgccct tcacggtatg ggggtgaaga agggcgaccg cgtcggcatc atgagccaga
     4141 acagctacga atacatggag ctgatcctgt cggtgacggc ggcgggatat gtcttcgttc
     4201 cggtgaactt caggctggca gggcgggaga tggcaggggt cctgtcggac gccgggcccg
     4261 tggtcctttt cgtccacgag caatacgtca agacggcgca tgagataaag ggtgagatcc
     4321 cctcggtgcg tgagtacgtg catatagggc ctgcggagac gcggcccgac ggatggcgcg
     4381 gatacgagga gatgatccag tgcgccgccc cgatgggatg ggtggagtcc gttgaggaag
     4441 gcgacctcgc catgctcatg tacacgagcg ggacgacggg cgcgcccaag ggtgtcatgc
     4501 agacccagct caacctctac cactgcggac ggacctgtgc cttcaacaac tgcatcgaca
     4561 ccgaggacat cggcttcacg atatgcccca tgtatcatgt cacggcaacg acatcctttt
     4621 tcggtccctt ctaccgggga gcgacaagca tcctcttcca gaagttcgac gccgaggccc
     4681 tcttcgaggc ggccagggag gagcgcatcg tcgcggggat gctcgcgacg cccatggtcc
     4741 gcatgctcct cgacgtgtgg ccctcggtga aggaccgcta cgacgtgtcg agcatgaaga
     4801 agctctggtt cgccggggcg ggcatcatcc ctgccgtcta caagcagttt atcgacacct
     4861 tcggctgcat cctcggcgag caccacggca ccacggagac gacgggggtg acggcgaacc
     4921 tctcggcgaa ggacattgcg aaggaactca ccgacgacaa cctgcgtatc ctggaatcct
     4981 gcggaaggga cgcctacgac tgcgaggtcc tcatcgtcga cgacaacgac aaccccgttg
     5041 tcccccccgg cgaaggggag atgaaggtgc gcggcctcgg cacgtcgctc ggctactggc
     5101 gcaaggagga acagacgaag aaggccttcc ggaacggctg gttctacacc gaggacatct
     5161 gccacatcga cgagaagggc tacatttata taattgaccg caag
//
LOCUS       DAOQTR010000079         5203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17589_length_5203_cov_3.244755, whole genome shotgun sequence.
ACCESSION   DAOQTR010000079 DAOQTR010000000
VERSION     DAOQTR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17589_length_5203_cov_3.244755"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..278)
                     /locus_tag="PKN85_02950"
     CDS             complement(15..278)
                     /locus_tag="PKN85_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43389.1"
                     /translation="MKKDVTICFRTNEELKQTLEKIAEVERRSLSSIIENMLHSQVKD
                     KVELKGSKKERRRYARKEVSLPAFVFKRDAKESTLQTGSTWRP"
     gene            704..1687
                     /gene="galE"
                     /locus_tag="PKN85_02955"
     CDS             704..1687
                     /gene="galE"
                     /locus_tag="PKN85_02955"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNT43390.1"
                     /translation="MKIFVTGGAGYIGSHVVKLLGEQGHNVLVYDNLSTGHEWAVLAG
                     RLVKGDLSDRQALKDTLREYSPDAVMHFAASIQVEESVREPLMYYRNNVINSINLLEA
                     MTELKIGYFIYSSTAATYGAPTVSPVDEDTPLTPINPYGMTKVMVESVLRDLAAKGDL
                     RYIALRYFNVAGADRQARIGQAYREATHLITRALRTAQGQQEKLFVFGTDYPTTDGTC
                     VRDYIHVEDLASAHLLALMYLRERGASDVMNCGYGHGFSVLDVVRAAKAVTGVDFPVE
                     LAGRRPGDPPELVADSSKIRRLTGWSPLYDDLGFIIETAWKWEKKRSATKD"
     gene            1698..2264
                     /locus_tag="PKN85_02960"
     CDS             1698..2264
                     /locus_tag="PKN85_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNT43391.1"
                     /translation="MRTFAAAVVVILAFAGLVVYAPSYLFYADPPRKSDAVVLFLGNE
                     YKERRAEAVKLIGEGYAAYLVIPAYGRMVEAGKHGEASRNVVPSRPSHYPVVYEDTHV
                     EIIEAKKLMDRKGLTSAIFVSSPHHMRRIKLIADRVFIGGAYVTTFVPTRFEKPGQGF
                     WFLRESDIRKVTSEYGKILWFFLYRWMK"
     gene            complement(2696..3484)
                     /locus_tag="PKN85_02965"
     CDS             complement(2696..3484)
                     /locus_tag="PKN85_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HNT43392.1"
                     /translation="MTNIISAAEITGAADLMNAYMSGGFLADFVTVMASSMALSGGNA
                     LIIALAALTLPPARRLQGVVLGTVAAILARVLIACLPAALLNVPCLKLCCGLLILTIG
                     LKLILDGHSPQEKKEVSGGSMKAFLMIILADLAMSPDNILAMAGASTGNPALAFLGLS
                     FSIPMVAFGGGVMSRLMGHYPPAIYAGAAILGKVAADTVLADPLTLRLLHAPGPALRY
                     GAEMLVAVGVVAAGIFLVRLREMRHPPVPAPHSGPFHVTGWDIR"
     gene            complement(3667..4314)
                     /locus_tag="PKN85_02970"
     CDS             complement(3667..4314)
                     /locus_tag="PKN85_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT43393.1"
                     /translation="MKIRIVVADDHKIMREGLKALIDKQPDMEVAAEAQDGLTATRLA
                     RKLLPHVIIMDIGMPEMNGIDATREIVAENKDVKIIALSMHSDRRFVLEMLKAGASGY
                     LLKDSAFEELVTAVHTVMTGQSYLSPRITDIVVKEYLHNLPRNDSTVFTILTQREREV
                     LQHLAEGKTTKQIASTLNLSVKTVETHRQQIMDKLQIRSVAELTKYAIREGITSL"
     gene            complement(4324..>5203)
                     /locus_tag="PKN85_02975"
     CDS             complement(4324..>5203)
                     /locus_tag="PKN85_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNT43394.1"
                     /translation="LGRKIKDRLEREGRVRGFEARWKMRDGSQMHVRENARAIRDSSG
                     RTLYYEGTVEDISEQKQAEERLDQYQRQLRSLASELSLAEERERRRISNLLHDHIGQL
                     LATAKMRLGWIESAVKDENIEREIDQIRDHIGQAIQFGRSLTFELSPPILYDLGLEAA
                     LEWLAEQVENQNGIHREFESDGLEKPIRDEIRVLLFTAVRELLANVVKHSGATRVKVT
                     VRRVDENISIHVADDGRGFNASKRSYHIAEARGFGLFSIRERLQSLGGHMDVRSQVGR
                     GARIILVAPLKREQEN"
BASE COUNT          968 a         1591 c         1527 g         1117 t
ORIGIN      
        1 catgtcttcg gcccctacgg ccgccaagta gaacccgtct gcaaggtcga ctccttcgcg
       61 tcccgcttaa agacgaaggc gggaagcgat acctccttgc gggcgtaccg cctgcgctcc
      121 tttttggagc ccttcaattc aaccttgtcc ttgacctgcg agtggagcat gttctctatt
      181 atggacgaaa gcgaccgtct ttcgacctcc gctatctttt ccagtgtctg cttcaactcc
      241 tcgttggtcc gaaaacatat cgttacgtct ttcttcattg ccgtccccct tacactgcgc
      301 tatctggcgc aaacctcgca gcattttgcg atacatcagt ataacataac atggaactat
      361 tgaaaagctg aacttgaccg caaaagggtc cttaacgcct tatataatgc gggaaaggcg
      421 gcatcccccg ggcgtccggc acgtggcatt cttccccttc ttcccgccgg gcccggaccg
      481 ggaaacaatt acttatcgtg agcaattgtg gtacaatagt gctcaatcgg aaccgggcag
      541 atatcaggac ggggacacgc ccgctgtggc gtttttcatc attccggccc cgggcaaccc
      601 gggaaggggg gaagatccat cgggtttctc ccatgccgcc gcgctctttc gttgcgccgg
      661 cgcagtcggg gagatgacgg caggtcccgc aaaggaggga cgaatgaaga tctttgtcac
      721 gggtggtgcc ggctatatcg gcagccacgt tgtaaagctc ctcggagaac agggacacaa
      781 cgttctcgtc tacgacaatc tttccacggg ccatgaatgg gccgtgctgg cggggcgcct
      841 cgtcaagggc gacctttccg acaggcaagc tctcaaagac accctgaggg aatactctcc
      901 cgacgctgtc atgcatttcg ccgcgtccat ccaggtggaa gaatccgtcc gggaacctct
      961 catgtactac cggaacaacg tcatcaacag catcaacctc ctcgaggcga tgaccgagct
     1021 caagatcggg tatttcatct attcgtcgac ggccgccacc tacggggcgc cgacggtgag
     1081 ccccgtcgat gaggatacgc cccttactcc catcaatccc tatggcatga cgaaggtcat
     1141 ggtcgagtcg gtgcttcggg acctggccgc gaagggggac ctccgttata ttgcgctgcg
     1201 gtatttcaat gttgccggcg ccgaccgtca ggcgcggata ggtcaggcct atcgcgaggc
     1261 gacgcatctt atcacgcggg ccctgaggac ggcacagggc caacaggaga agctctttgt
     1321 cttcggcacc gactacccca cgacagacgg gacctgcgtg agggactaca tccatgtgga
     1381 agacctggcg tccgcgcacc ttctggccct catgtacctg agggaaagag gcgcctctga
     1441 tgtgatgaac tgcgggtatg gccatggatt ctccgttctg gacgtggtcc gcgcggcaaa
     1501 agcggtgacg ggggtggact tccccgtgga actggcgggg cgcaggcccg gcgacccacc
     1561 cgagctcgtt gccgacagct cgaagatcag gaggctgacg gggtggtctc cactctacga
     1621 cgatcttggc ttcatcatcg aaacagcatg gaaatgggaa aagaagaggt cggcgacgaa
     1681 ggattgaagg atcacgcatg aggacctttg ccgcggccgt tgtcgtcatt ctcgcttttg
     1741 ccggccttgt ggtctacgca ccgagctacc tcttctatgc cgatcctccc cggaagtcgg
     1801 atgccgtcgt tctcttcctc ggcaacgagt acaaagagcg cagggccgag gcggtgaagc
     1861 tcatagggga aggatacgcc gcctatctcg ttattcccgc ttacggcagg atggtcgagg
     1921 cgggcaaaca cggcgaggcg tcgcgcaacg tcgttccctc gcggccctca cactaccccg
     1981 tagtctacga ggacacccat gttgagatca tcgaggcaaa gaagctgatg gaccggaagg
     2041 gtctgacctc cgccatattc gtcagctccc cccaccacat gaggcggata aagctcatcg
     2101 ccgacagggt cttcattggc ggagcctatg tcaccacctt cgttccgacc cgtttcgaga
     2161 agccgggaca gggtttctgg ttcctgcgcg agtcggatat aaggaaggtc accagcgaat
     2221 acggcaagat cctgtggttc tttctctaca ggtggatgaa gtagaacacg gtccggcgtc
     2281 ccaacccaaa aacgccatcg ataggtccta tccgtcctat tggtcttata cgtcccgtgg
     2341 gtcttaccac ttcctatcgg tcctatctgg actagtacca gtggggaatt tgcggtggag
     2401 gccgggatcc tgattgtaac gcagggcaag ggcgattgga tcccgacctc cgtcggggta
     2461 ctgatgaacg tttgctgcgc ggccgcgatg ggccttttac atcttatccc tttctcacag
     2521 gaccggcagg ggctgcgacg ctggacccaa ataccgtctt tccggcgtct tgaagcttcc
     2581 tacccccttc tccctgcagc gtccttcccc gatcggtcct gtgggcgcta gtccgcgcgc
     2641 tgaacggtcc tccttttttc tcccatcgcc cgcaccctcc cgaggtggag cgacgtcatc
     2701 gtatgtccca acccgtcacg tggaaaggcc ccgaatgagg ggcagggacg gggggatgcc
     2761 tcatctcccg cagcctgaca aggaagatcc ccgcggcaac gacgccgacg gccaccagca
     2821 tttcggcgcc ataccgcagt gcgggacccg gagcatgcaa cagtctcaag gtcaatggat
     2881 cggcaaggac agtgtccgcc gcgaccttgc caagaatggc cgcgccggca tagatcgcgg
     2941 ggggatagtg tcccatgagc cgcgacatca cacctccgcc gaatgccacc atgggtatgc
     3001 tgaagctcag cccgagaaag gccagggccg ggttgcccgt gctggcgccc gccatggcga
     3061 ggatgttgtc gggactcatc gccaggtccg cgaggatgat catgaggaag gccttcatgg
     3121 acccgcccga aacctccttt ttctcctgcg gtgaatgacc gtcgaggatg agcttcagcc
     3181 cgatggtaag aatgagaagt ccgcagcaaa gcttcaggca ggggacattg agaagcgccg
     3241 cggggaggca ggcgatgagg accctcgcca ggatggcggc gacggttccg aggacaaccc
     3301 cctgaagccg ccgggcagga ggaagcgtca gggcggcaag ggcaatgatc agggcgttcc
     3361 ccccggagag cgccatgctc gacgccatta ccgtcacgaa gtctgcgagg aaccctccgc
     3421 tcatgtaagc gttcatgagg tccgcggctc cggttatctc cgctgcgctt ataatgttgg
     3481 tcacggtctc gctgtctcct cgcccctgat acggaaccat agtatatcct tgcagaaggg
     3541 gtgaaggtat cggggttttc ctgattttcg ggggtatttt ccctgagacg cacgctggcg
     3601 cgaccaccgc gtctttcggc ccttatcccg gtcgaagagg gctcaaggtc ttccttcgcg
     3661 gggtctctac agggaggtta tgccttccct gatggcgtac ttggtcagtt ccgcgacgct
     3721 gcgtatctgg agcttgtcca tgatctgctg cctgtgggtc tccaccgtct tgacgctgag
     3781 gttcagagtg gaggctatct gtttcgtggt cttgccctcg gccaggtgct gcagcacctc
     3841 cctctcgcgc tgggtcagga tggtgaagac ggtggagtcg ttccggggaa ggttgtggag
     3901 atactccttg accactatgt cggtgatcct gggactcagg taggactggc cggtcatcac
     3961 agtgtggacg gcggtgacga gctcttcgaa cgcgctgtcc ttcaggagat agcccgatgc
     4021 cccggctttg agcatttcga ggacgaacct cctgtcggaa tgcatggaca gggcgatgat
     4081 cttcacgtcc ttgttctccg ccacgatctc gcgcgtcgcg tctatgccgt tcatttccgg
     4141 catcccgata tccattatga tgacgtgcgg caggagcttg cgggcgagtc ttgttgccgt
     4201 cagcccgtcc tgggcctcgg cggccacctc catgtcgggc tgcttatcga tgagggcctt
     4261 caagccttcc cgcatgatct tgtggtcatc ggccaccact attcttatct tcattgtcga
     4321 cccctagttc tcctgttccc gtttgagggg agccacgagt attatgcgcg cccccctgcc
     4381 gacctgggaa cgcacgtcca tgtgcccccc cagggactga aggcgctcgc ggatgctgaa
     4441 gagcccgaag ccgcgcgcct ccgctatgtg gtagctgcgc ttcgaagcat tgaagcccct
     4501 gccgtcatcg gcgacatgga tcgatatgtt ctcgtccacc ctgcgcaccg tcaccttcac
     4561 gcgcgtcgct ccggaatgct tgaccacgtt cgcaaggagt tcccgaacgg cggtgaagag
     4621 gagcacccgg atctcgtccc gtatgggctt ttcaaggcca tcgctctcaa actccctgtg
     4681 gatcccgttc tggttctcaa cctgctccgc cagccactcg agggcggcct cgagccccag
     4741 atcgtacagg atcggggggc tgagctcgaa ggtgagcgaa cggccgaact ggattgcctg
     4801 accgatgtga tcgcggatct ggtctatctc cctttcgatg ttctcgtcct tgacggcgct
     4861 ctcgatccat ccgagcctca tcttggcggt ggcgagaagt tggcctatgt ggtcatggag
     4921 gaggttcgat attcgtctgc gctcccgttc ctcggcaaga gagagctccg acgccaggga
     4981 acggagctgg cgctggtatt ggtccaatct ctcctccgcc tgtttctgct cgctgatatc
     5041 ttcaacggtg ccttcatagt agagggtccg cccgctggag tcccgtatgg cccgcgcgtt
     5101 ctcccgcaca tgcatttgcg acccgtcgcg cattttccac cgggcttcga aaccacggac
     5161 gcggccctcc cgctccagcc gatcctttat cttcctgccg agg
//
LOCUS       DAOQTR010000080         5178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17689_length_5178_cov_4.485458, whole genome shotgun sequence.
ACCESSION   DAOQTR010000080 DAOQTR010000000
VERSION     DAOQTR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17689_length_5178_cov_4.485458"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..916)
                     /locus_tag="PKN85_02980"
     CDS             complement(209..916)
                     /locus_tag="PKN85_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43395.1"
                     /translation="MAQERYIAVYPPALKLPAGGNPATHALEQGMAAWSQQLQQWLSQ
                     EVPKILGQATEVTEYVTTDELSQLHESGVVKADLVKLHDITVEAARINTIGKIKARAY
                     RNAAQSIAGTTNEKILIDTIDFDTENVVDVTTNNRIIPNLAGYYQVSGNARLGDIPDS
                     ETFYCSIFKNGERVSGGAYFRSGSMGQGVSTVSDLVYMNGTTDYLELYIYNSYSSALP
                     LVVGQSFQNYISIVGPF"
     gene            complement(918..2678)
                     /locus_tag="PKN85_02985"
     CDS             complement(918..2678)
                     /locus_tag="PKN85_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43396.1"
                     /translation="MNPFSIKPFTKGVLKDIPPQSMPVGGLTDARGIRITDAYVERRK
                     GYRRINDCESSDYILGVTQFRDLSGQTYIFFGDRNYLYKLPFGITREWDDGYPWWDGI
                     GYAWDPLNAGIYRITPNGEIPVCPFSSVSLSESSSSMSSSVAGTPESFTGRDRRIDQN
                     DATKWSFAQWGNNLLASNYEEPIQIIPGAGFDRHRTLVSHGLRARIVDVFAKHVIALN
                     TVDELDGAVPNRWWWSGLGNAEDWRYDDPASEGGFQTLQPNSQPITGGAALRDSYVIF
                     QEHMTHLVNYVGGTLVFAKQVVNSRIGALSQGLVQSAGDVVIFFGQNNIYRFDGYSFD
                     AIGEGNNRWIFKGLNMAQVSQSFSFIDRSTKEAWFVIPHYGDRPNLACIYDITNNLWT
                     FEDIDASAGCTQDGLDYPTIARTQFGISSSSSSSASSSSSSSMSSLSSSSSSSSSESS
                     SSSSQSASSSSYVEADADAKSYLMEVGVTDNDDDYPRSSYFITGEYDLGMPNRIKEIA
                     EVWPVVEELSNDIHISIGSRGRISDDIVWEELEPYTDQEVMGTRSYGVYLSFRVEADG
                     LDDFFRLSEICGYARAGGRR"
     gene            complement(2675..3322)
                     /locus_tag="PKN85_02990"
     CDS             complement(2675..3322)
                     /locus_tag="PKN85_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43397.1"
                     /translation="MDYSNFITNIGQWINRRHGEADDIIAQQIVESQYELEKKFPLWF
                     LIDEYTQVIPAGATSTKLPNHIVRVLDAEILDSNSFSYPLMFGTPAIIRDRYPSFQST
                     NPIKDRPQVAYAMGHVIRFAPQADAQYTLRFWAHHHLDPLDLTTNTSNVWTTTYLATL
                     RMKVLVDLEAFLKDDERIPVWKTRLNELLDDLESETRDMENVGMREVMTDTEDVY"
     gene            complement(3374..4420)
                     /locus_tag="PKN85_02995"
     CDS             complement(3374..4420)
                     /locus_tag="PKN85_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43398.1"
                     /translation="MQWYFQANKAGYLHPQKMSNKLRYYLTPKLRFRQFCDLKEALGK
                     NAGDSVDFNIVTNVTSGANVMGIREKDNMPETGFRVKQGSVCVVEFGNSIPFTGKSKV
                     LSKWDVEVIIRKLLARDAANTIDSRIELEFDNTLMRYVGTSATAGTMFRNGYAGIANA
                     TGMYPYHVKEIIDDLRTREVPTYDDEDYVCLATTFALRNLKDELEKVGMYTESGRKPI
                     LAGEVGRYYGCRFVEVNHGMSAESFDVGKSSEAYFFGSDTVIEAIAIPEEVRVKEPSD
                     YQRKQGLAWYGIFGYKLQWGDRRVATDDWHESRIIKWDSADGTNSSSASTYSRSYNSW
                     ASASESLGWCISPA"
     gene            complement(4445..>5178)
                     /locus_tag="PKN85_03000"
     CDS             complement(4445..>5178)
                     /locus_tag="PKN85_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43399.1"
                     /translation="HLRRPLRQASVPAKDGEVTIAELQKSYQVQGHLTRQLQQVAAYK
                     QQLDNVGAQLQAKARELDEQFRAYEEQLFTPEEVQQRKARRDREQAERGLQYQNLFMQ
                     CRNTLYQRHPDADNLDYDPDFVDFRAKNMPFLNEQLLNNYGPGVFFPAMDIAMSYYKD
                     VKKCMEALQQAQDATTSFKKEREAELAKREKEQRAEKKKAKDVKPSTVDAKKGGDEDD
                     EAENPASNRDYVRSLAKKRLAAQGL"
BASE COUNT          949 a         1584 c         1450 g         1195 t
ORIGIN      
        1 agcttcacaa ggtccgcctt cacaacaccg gattcggaaa gcaagtccgt aagcgccttc
       61 agatacgcga aattctccct gacctccttc gagttcggac tcgacctgta ttccggtttc
      121 ccttcgtgcc atgccatagt tgttcctcct tgcttatgga tgatgccggt gcttgtcttc
      181 ccgcttttcc gctgtcattc atgctccctc agaacggccc cacaatggag atgtagttct
      241 ggaacgattg acccactaca agcggcaagg ccgatgaata gctgttataa atatagagtt
      301 ccaggtaatc cgtggtgccg ttcatataaa cgagatcgga aacagttgat acgccctgcc
      361 ccattgaccc gctacggaaa tatgctcccc ccgaaacccg ctccccgttt ttaaatatgc
      421 tacagtaaaa tgtctcgcta tcaggtatat cgccgagcct tgcattgcct gaaacctgat
      481 agtatcccgc cagattcggt atgatgcgat tgtttgtcgt aacatcgaca acgttctccg
      541 tgtcgaagtc gatggtgtcg atgagaatct tctcgtttgt cgttcccgca atactctgcg
      601 ccgcattccg gtaggctctg gctttgattt tcccgatggt gtttatccgt gccgcttcga
      661 cggtgatgtc gtgcagcttc acaaggtccg ccttcacgac cccggactca tgaagctggc
      721 ttaattcatc ggtggtgaca tactccgtga cctcggttgc ctgccccagg attttcggca
      781 cctcctggga gagccactgc tgaagctgct gcgaccatgc ggccatgccc tgctcaaggg
      841 cgtgcgtcgc ggggttgccg ccagcaggca gcttcaatgc cggtggatat acggctatgt
      901 accgctcctg cgccatatta ccttctccct cccgcgcgcg cgtatccgca gatctccgag
      961 agccggaaaa agtcgtccag accgtccgcc tctaccctga acgaaaggta cacgccgtag
     1021 gatcgcgtac ccatgacctc ctggtccgtg tacggctcaa gctcctccca tacaatgtcg
     1081 tccgagatcc tgccccgcga gcctatcgag atgtggatgt cgttactcaa ctcctccacg
     1141 accggccaga cctccgcgat ctccttgatc cggttgggca tcccgaggtc gtactcgccg
     1201 gttatgaagt acgagcttcg cgggtaatcg tcgtcattgt ccgtcactcc cacttccatg
     1261 agatacgact tcgcgtccgc gtccgcttcg acgtagctgc tcgaagaggc gctctgcgaa
     1321 ctgctcgacg agctttccga ggagctgctc gacgacgagg acgagagcga ggacatggac
     1381 gaggacgacg aggacgatgc gctcgacgat gacgacgagc ttatgccgaa ctgcgtccgg
     1441 gcaatggtcg gatagtcgag cccgtcctgt gtgcagccag cagaggcgtc gatatcctcg
     1501 aacgtccaca gattgttcgt tatgtcgtag atgcaggcga ggttcggcct gtcgccgtag
     1561 tgcggaatga cgaaccacgc ctccttcgtg ctgcggtcga tgaaggagaa gctctgcgag
     1621 acctgcgcca tgttcagacc cttgaagatc cagcggttat tcccctctcc gatcgcgtcg
     1681 aagctgtacc cgtcgaagcg atagatgttg ttctggccga agaagatcac aacatctccc
     1741 gcgctctgca cgagcccctg agagagagcg ccgatacggg aattgaccac ctgctttgcg
     1801 aacacgagcg tcccgccaac atagttgacg aggtgcgtca tgtgctcctg gaagatgacg
     1861 taggagtcac gaagcgctgc gcccccggta atgggctgag agttgggctg gagcgtctgg
     1921 aagccgccct cgctcgcggg atcgtcgtat ctccagtcct cggcattgcc caggccgctc
     1981 caccaccatc ggttcgggac cgccccgtcc agctcatcga ccgtattgag cgcgatgacg
     2041 tgcttcgcaa acacgtccac gatcctggcc ctgagcccgt gcgaaacaag ggtccggtgc
     2101 cggtcgaatc ctgctcccgg gatgatctgg ataggctcct cgtagttgct cgccagcagg
     2161 ttgttgcccc actgggcaaa cgaccacttg gtcgcgtcgt tctggtcgat cctgcggtcc
     2221 ctgcccgtaa aggactcggg agtcccggcg actgaagacg acatggagga cgagctttcg
     2281 gacagactca cgctcgaaaa cgggcagacc ggaatctcgc cattgggcgt gatccggtag
     2341 atgcccgcat tgagcgggtc ccaggcgtac ccgatcccgt cccaccaggg gtagccgtcg
     2401 tcccattccc tcgtaatgcc gaacgggagc ttgtagaggt agttcctgtc tccgaaaaag
     2461 atgtatgtct ggccgctcag gtcccggaac tgcgtaaccc ccaggatgta gtcactgctc
     2521 tcgcagtcgt tgatcctgcg gtatcccttc ctgcgctcca cataggcgtc cgtgatccga
     2581 atgccgcgcg cgtcggtcag accgcccacg ggcatggact ggggggggat gtccttgagg
     2641 acccccttgg tgaagggctt tatggaaaac gggttcaata cacgtcctcc gtgtcggtca
     2701 tgacctcgcg catgccgacg ttctccatgt cgcgggtctc cgattcaagg tcgtcgagaa
     2761 gctcgttcag ccgcgtcttc cacaccggaa tcctctcgtc gtccttcagg aacgcttcga
     2821 ggtccacgag caccttcatc cgcagggtcg caagataggt cgtggtccag acgttgctcg
     2881 tgttcgtcgt caggtcgagc gggtcgagat ggtggtgcgc ccaaaatctc aaggtgtact
     2941 gcgcgtcggc ctgcggagcg aaccggatga cgtggcccat ggcgtatgcg acctggggcc
     3001 tgtccttgat cgggttcgtc gactggaacg aagggtatct gtcgcggatg atagcgggcg
     3061 tgccgaacat gagcggatag ctgaaggagt tcgagtcgag gatctctgcg tccaggaccc
     3121 ggacgatgtg gttcgggagc ttggtcgacg tggcgcctgc cgggatgacc tgcgtgtact
     3181 cgtcgatcag aaaccacagg ggaaacttct tctcaagctc gtactggctc tcgacgatct
     3241 gctgcgcgat gatgtcgtcg gcctcgccgt gccgcctgtt gatccactgc ccgatgttcg
     3301 tgatgaaatt gctgtagtcc atgtcggtcc ctctaaaacg gggagagggt ctcccctccc
     3361 cccgatggtt ttgttacgcg ggactgatgc accagccgag agactcggaa gcggatgccc
     3421 acgagttgta ggaccgggaa tacgtcgatg cggacgaaga gttcgtcccg tccgccgaat
     3481 cccatttgat gatcctcgac tcgtgccagt cgtcggtcgc caccctccgg tcgccccact
     3541 ggagcttgta cccgaagatg ccgtaccacg cgagaccctg cttcctctgg tagtccgaag
     3601 gctccttgac cctcacctcc tcgggaatcg caatggcctc gatcaccgta tcggagccga
     3661 agaagtacgc ctccgaggac ttgccgacat cgaagctctc ggcgctcatg ccgtggttta
     3721 cctcgacgaa acggcagcca taataccgac cgacctcgcc agccagaatc ggcttgcggc
     3781 cagactcggt atacatgccc accttctcca gctcgtcctt caggttgcga agcgcgaacg
     3841 tggtggcgag acacacataa tcctcgtcgt cgtaggtcgg gacctcccgc gtgcggagat
     3901 cgtcgatgat ctccttgacg tggtaggggt acatgccggt cgcgtttgcg atgcctgcat
     3961 agccgtttct gaacatcgtc cctgcagtcg cgctggttcc gacataccgc atcagggtgt
     4021 tgtcgaactc cagctcgatc ctgctgtcga tggtgttggc cgcgtccctg gcgagcagtt
     4081 tgcggatgat gacctccacg tcccacttgc tcagaacctt ggacttgccg gtaaacggaa
     4141 tggagttgcc gaactcgacc acgcacaccg aaccctgctt gacccggaat ccggtctcgg
     4201 gcatattgtc cttctcccgg atacccatga cattcgcccc ggacgtgacg ttcgtgacga
     4261 tgttgaagtc aaccgagtcg ccagcgttct tgccgagcgc ctccttgagg tcgcagaact
     4321 gcctgaacct cagcttcggc gtcaggtagt acctgagctt gttggacatc ttctgcggat
     4381 gaagatatcc cgctttgttt gcctgaaaat accactgcat aggattttcc cctttcttat
     4441 tgtcttacaa tccttgtgca gcgagccgct tcttcgccag cgatctgacg taatccctgt
     4501 tgcttgctgg attttcagct tcgtcatcct cgtcgccgcc cttctttgca tcgacggtag
     4561 acggtttgac atctttggcc ttcttctttt cagccctctg ctctttctcc ctttttgcca
     4621 actctgcctc acgctccttc ttgaacgagg tggttgcgtc ctgggcttgc tggagcgcct
     4681 ccatgcactt cttgacatcc ttgtagtagg acatcgcaat gtccatggca gggaaaaata
     4741 ccccagggcc atagttgttg agcaactgct cgttcaagaa cggcatgttt ttcgctctga
     4801 aatctacgaa gtctggatcg tagtccagat tgtccgcgtc ggggtgccgc tgatacagcg
     4861 tgttgcggca ctgcataaag aggttctgat actgcaagcc cctctcggcc tgctcccggt
     4921 cccgcctcgc cttgcgctgc tggacctcct cgggcgtgaa aagctgctcc tcgtacgccc
     4981 tgaactgctc gtcaagctcc ctggccttgg cctggagctg tgcgcccacg ttgtcgagtt
     5041 gctgcttgta ggcggcgacc tgctggagct gccgggtgag atgcccctga acctgatagg
     5101 acttctgaag ctccgcgatc gtgacctcgc cgtccttcgc ggggactgac gcctgcctca
     5161 gaggtcttcg gaggtgag
//
LOCUS       DAOQTR010000081         5178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17691_length_5178_cov_3.705641, whole genome shotgun sequence.
ACCESSION   DAOQTR010000081 DAOQTR010000000
VERSION     DAOQTR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17691_length_5178_cov_3.705641"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKN85_03005"
     CDS             complement(<1..673)
                     /locus_tag="PKN85_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNT43400.1"
                     /translation="MNFRTRLLIVVAVMIAVSVFLSSATTYVSARRHLEAAARKQMEQ
                     TVALIAKQSQISLERFKMDMELLAESRLVRDVTRSPKNEALVREMNLRFLDIVRKNNV
                     YTSINLIDRDAWCIASSYPDRIGYSPMRQMVATRSDFKAAIAGEATISQVILSHGTGR
                     PIIAISVPVREGGRVMAVVRSTLDLDHINDHFLAPQETILGAKAYVYDPWLDMNLPKG
                     WVADTK"
     gene            1150..1818
                     /locus_tag="PKN85_03010"
     CDS             1150..1818
                     /locus_tag="PKN85_03010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT43401.1"
                     /translation="MKFEGVVFDLDGTLVNSLEDIADSMNTVLREHGFPTHGLTEYKA
                     FVGRGMRNLVVRSLPEDARDEARIALCRDSLLKEYGRNCLKKTRPYEGITDLLDGLDR
                     RKMNRAVFSNKIDHLTKRIVEALFPGRSFPVVVGAGAGMPEKPDPTGVLSVARQLGIE
                     PERLIYVGDSDVDMETARNAGMYPAGALWGFRTEEELVAHGAGSLLEHPLDLLRIIDG
                     KSRA"
     gene            1912..2589
                     /locus_tag="PKN85_03015"
     CDS             1912..2589
                     /locus_tag="PKN85_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT43402.1"
                     /translation="MRILLVEDDEKIASFIIKGMKAAGCAVDHVTDGREGLTLATTEP
                     YDAAIVDIMLPGLDGLSVVGEMRKGKVATPVIILSARGSVEDRVKGLQTGADDYLTKP
                     FAFTELLARVQALVRRASGITEPTRLAVGDLSVNLVSREVKRAHKTIELQPMEFSLLE
                     YLMRNAGRVVSKTMIMEHVWNYDFDPQTNVVEARICRLRDKIDRDFPGKLIHTVRGVG
                     YVLRENP"
     gene            2567..3988
                     /locus_tag="PKN85_03020"
     CDS             2567..3988
                     /locus_tag="PKN85_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43403.1"
                     /translation="MSSGKIRKTRDTLALRLTLWYAGIFTVSSFVAFFLFYTLVTSVV
                     RGRTDEELMNQARRYVVLASTEGLEEAKKAAVVETQAAGLRKVFFRMLYADGVTFSSS
                     NMSYWKDIGIHVNAIRQLVEGADHVFETMDLPERRDEVRILYVTLGPNIILQIGEAVE
                     GSSRFLEAFRRIFIVTMGFLIVLAAGIGWFMARRAVSGVEAVTRTAREISAGTLGRRV
                     PVNMRGDEIDQLAVTFNEMLDRIEALVREMKEMSDNIAHDLKSPITRIRGAAEVTLST
                     SGSPEEFENMAASTIEECDRLLDTINTMLMISRTESGVDRPPQERIKTAELVREACDL
                     YEPAASDKGIVLTCEIEEAVDVAGDKRMIRRMVANLIDNAIKYTPSGGSVDVRLSRDG
                     SNRIALTVSDSGMGISPEDLPRIFDRFYRGDRSRSEAGLGLGLSLARAIARAHGGDIT
                     VTSIPDRGATFTVTLPKKTAETA"
     gene            4159..>5178
                     /locus_tag="PKN85_03025"
     CDS             4159..>5178
                     /locus_tag="PKN85_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT43404.1"
                     /translation="MERTRVIRAVVIVLATIALTVGLGYGVSKAVKGTNNTPVPSKSS
                     AVPIMVPGNFAELAEKARPSVVNIQTVKTLKGNGRVFRHFFGNPFGKESPFGGQDPFE
                     EFFGRRGGNGSPEDFRQKSLGSGFIVDGDGYIVTNNHVIDGANEIKVKLTDGREFDAS
                     IVGRDSKTDLALIRITGASNLTPITMGNSDELKVGSWVVAIGSPFGLEQTVTAGIVSA
                     KGRIIGSGPYDNFIQTDASINPGNSGGPLINMKGEVVGINTAIVASGQGIGFAIPVDT
                     ARNVIAQLKGNGRVTRGWLGVSIQEITPALASSFGLADRKGALVADVIKDGPADKAGI
                     AQGDVI"
BASE COUNT         1116 a         1438 c         1605 g         1019 t
ORIGIN      
        1 ccttggtgtc ggccacccag cccttgggca ggttcatgtc caaccacggg tcgtagacgt
       61 aggccttggc tccaagtatg gtctcctggg gagcgaggaa atgatcgttg atgtgatcaa
      121 ggtccagggt ggagcgcacc acggccataa cccttccgcc ttccctcacg gggacgctga
      181 tcgctatgat ggggcgcccc gtgccgtggc tcaggatcac ctgggagatc gttgcctctc
      241 cggcgatggc agccttgaaa tccgaacgtg tggcgaccat ctgacgcatg ggggagtatc
      301 ctatgcggtc gggataagag gacgcaatgc accaggcgtc ccggtcgatg aggtttatgg
      361 atgtatagac attgttcttc cttacgatgt ccagaaaacg taaattcatc tcccgcacga
      421 gtgcttcgtt cttcggggat ctggtgacgt cgcggacgag gcgtgattcc gcaagaagct
      481 ccatgtccat cttgaatctt tcaaggctta tctggctttg tttggcgatg agagccaccg
      541 tctgctccat ctgcttcctg gcagctgcct cgaggtgtcg tctggcggat acataggtgg
      601 tggccgagga caaaaagacg cttacagcga tcatcaccgc aacgacaatg agaagtctgg
      661 tgcgaaagtt catcgatccc cttctttccc gggcgaaggg ggtttttgac tggcctctta
      721 cggaggatcg cctgccccat gccccgtttc attatccctt ccactacgaa gaaaacggtc
      781 gatcaatgag gagattttac ggtacgatcc tgctcctcag gaccggtatg taacagagga
      841 gacgcagtac gggacgaatc ggccacattg cggtgcgaag atgatgtgga aatagccttc
      901 acactccggt tctcgagaac tgttgcagca cccgaagact gtaattgaca ggcacggcca
      961 ccccgggcgc acaccccgaa taagaaaagc gcgggcgggt gacggtacgc ggggcgggcg
     1021 ccgcagttgt tgtcattaag tgtttcaaag ctccacagtt ctatggttta actaaatgta
     1081 agaacccccc atcttcatga gtgaaacttt acccgtgaaa cggagggtca ggaaatggag
     1141 ggaggtatca tgaagttcga gggagtggtc ttcgatctcg acggaactct ggtcaattcg
     1201 ctggaggaca tagcggattc catgaacacc gtcctcaggg aacatggttt tccgacgcat
     1261 ggcctcacgg aatacaaggc cttcgtcggc agggggatga ggaatcttgt cgtgcgctct
     1321 ctgcccgagg acgcgagaga cgaagcccgc atcgccctgt gccgcgactc gttgctgaag
     1381 gaatacggca ggaattgcct gaagaagacc cggccctacg agggcatcac ggatcttctt
     1441 gacgggcttg acaggcgcaa gatgaatagg gccgtctttt ccaacaagat cgatcatctg
     1501 acaaagagga tcgtggaggc gctctttccc ggcaggtcat tcccggttgt cgttggggcc
     1561 ggggccggaa tgccggagaa accggatccc acgggtgttc tgtccgtcgc ccggcagctg
     1621 gggatcgaac ctgaacgtct gatatacgtg ggagattccg acgtcgatat ggagaccgcg
     1681 cgcaatgcgg gaatgtaccc ggccggggcc ctctggggtt ttcggacaga ggaagaattg
     1741 gttgctcacg gggcgggatc cctgctcgag caccccctcg atctgctcag gatcatagat
     1801 gggaaaagcc gggcatgagc cgcccgccga ttgacgaata tcccccgtct gtgcaaggat
     1861 aatgaagggt cccgtgtccg gtgacctgtg gcgggcccgc aggaaagcgc cgtgagaata
     1921 ctgctcgttg aagatgatga aaagatcgcg tccttcatca taaaagggat gaaggccgcg
     1981 gggtgcgccg tggaccatgt caccgacggc agggagggcc tcacgctggc gacgacggaa
     2041 ccctatgacg cggccatcgt ggacatcatg cttcccggcc tcgacgggtt gtccgtcgtc
     2101 ggggagatgc gaaagggcaa ggtcgcgacg cccgttatca tcttgagcgc ccggggttcc
     2161 gtggaagacc gtgtcaaggg gttgcagacc ggtgccgacg actatctcac caagcctttt
     2221 gccttcaccg agcttcttgc aagggttcag gcgctggtgc gccgagcgag cggcatcacc
     2281 gagccgacgc ggcttgccgt gggagacctc tcggtgaacc tcgtgagcag ggaggtgaag
     2341 agggcccaca agacgatcga actccagccc atggaattct cactcctcga atatctcatg
     2401 cgcaacgccg gcagggtggt ctccaaaacg atgatcatgg agcacgtctg gaactacgac
     2461 ttcgatcccc agacgaacgt ggtggaggcc aggatatgca ggcttcggga caagatcgac
     2521 agggattttc ccggcaagct tatccacacg gtgcgggggg taggctatgt cctcagggaa
     2581 aatccgtaaa acccgtgata cgctggccct gagactcacc ctctggtacg cggggatatt
     2641 caccgtctcc tccttcgtcg ctttctttct cttctacacg cttgtcacat ctgttgtccg
     2701 tggccggacg gacgaggaac tcatgaatca ggcccggcgc tacgtcgtcc ttgcgtcgac
     2761 ggaggggctg gaagaggcca agaaggccgc cgtcgtggaa acccaggccg ccggtttgcg
     2821 aaaggtcttc tttcgcatgc tctatgccga cggcgtgacg ttctcttcgt cgaacatgtc
     2881 ctactggaag gacataggca ttcacgtgaa cgcgatccgc caactggtgg agggtgccga
     2941 ccatgtcttt gagacgatgg acctgcccga gcgccgggac gaggtccgca tcctgtatgt
     3001 gacgctcggc ccgaacatca tcctgcagat cggcgaggcc gtcgagggat cgtcgcgatt
     3061 cctcgaggcc ttcagacgga tctttatcgt cacgatgggg tttctcatcg tccttgccgc
     3121 cggtatcgga tggttcatgg cgaggcgggc agtatcgggg gtggaggccg tgacgcgcac
     3181 ggcgcgggag atatccgcag ggacgcttgg tcgaagggtg cccgtcaaca tgcgcggtga
     3241 cgagatcgat cagttggccg tcaccttcaa cgagatgctc gaccgcatcg aggcgctcgt
     3301 gagggagatg aaggagatga gcgacaatat tgcccacgat ctgaagagcc cgataacgcg
     3361 aatacgggga gcggcggagg tgacgctgag cacatccggg tcccccgaag agttcgagaa
     3421 catggcggcg agcaccatcg aggaatgcga ccgccttctg gataccatca acaccatgct
     3481 tatgatatcg aggacggaat ctggagtgga ccgtccgccg caggagagga taaagaccgc
     3541 cgagctggtg cgcgaggcct gcgacctgta tgagcccgcc gcctccgaca agggtatcgt
     3601 tctgacctgc gagatcgagg aggcggttga cgtggcaggg gacaagagga tgatccggcg
     3661 gatggtcgcc aacctcatcg acaacgccat caaatacacc ccctccggcg ggagcgtcga
     3721 tgtccgcctt tcgcgggacg gctcgaaccg catcgcgctt actgttagtg acagcggcat
     3781 gggtatctcg ccggaagacc ttccccgcat cttcgacagg ttctaccgcg gcgacaggag
     3841 ccgctcggaa gcgggcctgg gcctgggcct cagcctcgcg cgggccattg cccgtgctca
     3901 cggtggagac atcaccgtga caagtatccc cgacagaggt gccaccttca ccgtaaccct
     3961 ccccaagaag acggccgaaa ccgcttgaac cgcttgaggg ccaaagagac ccgtccttcc
     4021 catgatccat aacccataac ctattacccg tcttcattac caatttgtaa tctcaccgtc
     4081 atgtcctgat gaacttcgcg atgtagactg gagtatcggc aaggcagcgc ctggccaata
     4141 tacatcaagg agacctgtat ggaacgaaca cgagtaatca gagctgttgt tatcgtactg
     4201 gcgaccatcg cccttacggt ggggctcggc tatggggttt cgaaggctgt caagggaacg
     4261 aacaacacgc cggtgccttc gaagagctcc gcggtgccca tcatggtccc cggcaatttc
     4321 gcggaactcg cggagaaggc ccgtccgagt gtcgtgaaca ttcagacggt caagacgctc
     4381 aaaggaaacg gacgcgtttt tcgccatttc tttggcaacc ccttcggcaa ggaaagcccc
     4441 ttcggcgggc aggacccttt cgaagagttc ttcggccgcc ggggagggaa cgggtcaccc
     4501 gaggatttcc ggcagaaaag ccttggctcg ggattcattg tcgacgggga cggttatatc
     4561 gtgaccaata accacgtgat tgacggtgcc aacgagataa aggtgaaact aactgacggg
     4621 agagagttcg acgcgagcat cgtcggcagg gattccaaga ccgaccttgc cctcatcagg
     4681 atcacgggcg cttcgaacct cacgccgata acgatgggca attcggacga actcaaggtc
     4741 ggcagctggg tcgttgcgat aggcagcccc ttcggtctcg aacagacggt gacggcaggg
     4801 atcgtcagcg ccaagggcag gatcatcggc tcagggcctt atgacaactt cattcagacg
     4861 gatgcctcca taaatcccgg aaacagcggt ggtcctctca ttaatatgaa aggggaggtg
     4921 gtcggaatca acacggcgat cgtggcctca ggacagggga taggctttgc gattcccgtc
     4981 gatacggcca ggaacgtgat cgcgcagttg aaaggtaatg ggagggtcac ccgcggctgg
     5041 ctcggcgtga gcatacagga gatcacccca gcactcgcct cctctttcgg gcttgcagac
     5101 agaaaagggg ccctcgtggc cgacgtgatc aaggatgggc ctgccgataa agccggaata
     5161 gctcagggcg acgtgatc
//
LOCUS       DAOQTR010000082         5143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17861_length_5143_cov_3.378931, whole genome shotgun sequence.
ACCESSION   DAOQTR010000082 DAOQTR010000000
VERSION     DAOQTR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17861_length_5143_cov_3.378931"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..958)
                     /locus_tag="PKN85_03030"
     CDS             complement(197..958)
                     /locus_tag="PKN85_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNT43405.1"
                     /translation="MDMRFKERFITLWEKYFPGAPLPVIFFYSDDEAGATVVKGPKSL
                     EEHRCVLADITRASRGRSLAFNVDSIGCGGGRKYLGFTEVLRPNFEYFLSCGIPGELE
                     GERYKKSPELVAEVMKKMPKFSAPARYIVFKRWDGIDKNDNPDAVIFFATPDVISGLF
                     TLANYDGVDPNGVFCPFGAGCSTIVTYPYLEKNARRPRSVIGMFDVSARPCVPANTLT
                     FATPFKRFVTMVGNMDESFLITESWKKVKRRIKKG"
     gene            1225..2523
                     /locus_tag="PKN85_03035"
     CDS             1225..2523
                     /locus_tag="PKN85_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT43406.1"
                     /translation="MRRYLFCVLAVFLMIVGSDLFAQEAPIRPGEPLNLERAIDIGLK
                     MNPAIMGFQYTMKAREAQLGQARAGYLPKVDGSAGFTRNFEVNNTRDPYYGALLDQYN
                     QNVANVSLKQMIFDFWRTPTSVNIGRLNVESSRRDIDNSMNTVANTVKSSYYGVLKAK
                     RSRDVNLEVVGQYAKHYRVAVVFFEAGKKPKYDVTKAELDLSNAKLNLIGAENDLKVA
                     WVNLNNAMGIDGDADYAIHDSLDFDRYEISLEDALKTAYETRPDLKSLEAQRESAEKA
                     VARAKQEYYPQINGNAQYNFAGSQYPLGQGWYAGVALSVNLFDGLSTTNKVAEAQANK
                     QSVDAKISAMKLQITLDVKQAWLALIKARQTIETTNVQIRQATENLEIANLRYDAGLA
                     TPLEVTDATVTYSQAKLANISALFDYKVARANMEKAMGSR"
     gene            2520..3788
                     /locus_tag="PKN85_03040"
     CDS             2520..3788
                     /locus_tag="PKN85_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT43407.1"
                     /translation="MMDAQKGRGVIGRLRAMSRRKKVVAAGIVLVCVVVIVYVATRKK
                     PAPPPERVAVTAAKAVAKDMPVELREIGTIEGYRSVPIYAQITGQLVKTHFKEGQDVK
                     KGQPLFTIDPNQYQAKVRQAEAQLARDTAQMKFARDEAKRYKYLYEKGAVALSDYENK
                     QSVADAQESLVVADRASLQDARLNLAHCFISAPFDGRMGAYAVFEGRMIKDVDTQLAT
                     INQISPVYASFSIAEKDLPAVRKYSVGQKLKARVLPAGYTGEPPEGELTFMDNTVNTQ
                     TGMILLKATFPNTDRFLWPGQFVNVVLTLTTEKGVVVVPERAIQLSQTGKYAFVIKAD
                     DTVEYRVVATDRTVRGLTVVRKGINAGETVVTDGHFKLRNGFPVSVRDSLASGQRGNG
                     GSLEAAPGKPAPQKTGTGAPPPEASAGTKK"
     gene            3785..>5143
                     /locus_tag="PKN85_03045"
     CDS             3785..>5143
                     /locus_tag="PKN85_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT43408.1"
                     /translation="MNISAIWIKRPIMTILVMFGILFFGINSYVDLPINNLPAVDFPT
                     IMVSASLPGASPETIASNVAKPLEKQFSTIQGLSSMSSQSYTGSTSIILQFTLDRNID
                     ACAQDVNSKIAAAQGDLPTNLPSPPTYEKVNPSDQPILYFALVSETMPLTQLNDYAEN
                     YLAQNFSMVNGVSQVITYGQKFAARIQVNPKLLAKQGIGIDDVERAIKAANVNLPGGT
                     IDGSFQAFTIDSNGQLTVGEAYRDVIITYKDGTPVRVKDVGDAISGVEKDKQMAAWYS
                     TREKTRRAIVLAVKKQPGSNTVLVAQGVKELVPRLKSMVPESVEWHLLYDASDYIEES
                     IQDVQFTLVLTIIIVLFVVFVFLRSFRSTVIPNVAVPLSIIGTFAVMSVLGFSLNNLS
                     MMALVLCVGLVVDDAIVVLENIVRRMEMGENATEASFKGSQEIGFTIMSMTLSLVVVF
                     IPL"
BASE COUNT         1135 a         1505 c         1500 g         1003 t
ORIGIN      
        1 cacatcgggg ttcaatagag acgcacacgg gtcggcgctc acgcgcctaa cgggcatacg
       61 ggtctgccct ccgggcaaac gggcacacga gtaaacgata tcattatctc tccttattct
      121 ttatccttat acccgttcac tcgttaggcg cgtgagcgcc gacccgtgcg cgcgaagcgc
      181 cgacccgtgc gcgcgttcag cctttcttta ttctcctttt caccttcttc cagctctccg
      241 ttatgaggaa gctctcgtcc atgttgccca ccatggtcac gaacctcttg aaaggggtgg
      301 cgaaggtgag ggtgtttgcc gggacgcagg ggcgggcgga gacgtcgaac atgccgatga
      361 cggacctcgg ccggcgggcg ttcttttcga gataggggta ggtgacgatg gtggagcagc
      421 cggcgccgaa ggggcagaag acgccgttgg gatcgacgcc gtcgtagttc gcgagggtga
      481 agagccccga aataacgtca ggagtggcga agaagatgac ggcgtcggga ttgtcgttct
      541 tgtcgatccc gtcccaacgc ttgaagacga tgtagcgggc gggagcgctg aatttcggca
      601 tcttcttcat cacctctgca accagctcgg gggacttctt atatctctcg ccttcgagct
      661 ctccaggtat tccgcaggag aggaagtact cgaaattggg gcggaggacc tctgtgaagc
      721 cgagatattt cctgccgccg ccgcagccga tggaatcgac gttgaaggcg agcgaccggc
      781 cccgtgaggc ccgggtgatg tcggcgagga cgcagcggtg ctcctccagg ctcttcggcc
      841 ctttcaccac ggttgcaccg gcctcgtcat cactgtaaaa gaagatcacc ggcagtggtg
      901 ccccgggaaa gtatttctcc cacaacgtga tgaatctctc cttgaatcgc atgtccatga
      961 atgacctcct ccttttttag aagacggttt taagttttag gttttaggtt ttaagccgaa
     1021 gcaagggtga ttcatgcaga tcctggcact taaaacttaa cacttaaaac ttaaaaccgt
     1081 tttccattct atcacgtttc ttccggtgtc caaagtcctt cttgacaacg gtggtccact
     1141 tatctaatat gtcacgtacc tgttcatcaa acagacccct tttcgcttga gaaagagccg
     1201 gtcagaggcg agagtagact ggaaatgaga agatacctgt tctgtgtact ggcggtgttt
     1261 ctgatgatcg tcgggagtga cctctttgcc caggaggcgc ccatcaggcc gggcgagccc
     1321 ctcaacctgg agagggccat agacatcggt cttaagatga acccagccat catgggcttc
     1381 cagtatacga tgaaggcgcg cgaggcccag ctcgggcagg cgcgggcagg ttatctcccg
     1441 aaggtcgacg gctcggcggg gttcacgcgc aacttcgagg tgaacaatac ccgtgaccct
     1501 tattacgggg cccttctcga ccagtacaac cagaatgtcg ccaacgtgag cctcaagcag
     1561 atgatcttcg atttctggag gacgccaacg agtgtgaata tcggcaggct caacgtggaa
     1621 tcgtcccgcc gcgatatcga caacagcatg aacaccgttg ccaacacggt gaagtcctcc
     1681 tactacgggg tcctgaaggc caagcgttcg cgggacgtca acctggaggt cgtcgggcaa
     1741 tacgcgaagc attaccgcgt agccgtcgtc tttttcgagg cgggcaagaa gccgaagtac
     1801 gatgttacga aggccgaact cgacctttcc aacgcgaaac tgaacctcat cggagccgag
     1861 aatgacctca aggtcgcctg ggtgaacctc aacaacgcca tgggcataga cggcgacgcg
     1921 gactatgcca tccacgacag tcttgacttc gacaggtacg agatctccct ggaggatgca
     1981 ctgaaaaccg cctacgagac gcggcccgac ctcaagtccc tggaggccca gagggagtcc
     2041 gccgaaaagg ccgttgcccg ggcgaagcag gaatactatc cgcagatcaa tggtaacgcc
     2101 cagtacaact ttgccggcag ccagtacccc ctcgggcagg gctggtatgc cggggtcgct
     2161 ctgtcggtca acctctttga cggtctgtcg acgacgaaca aggtcgccga ggcccaggcg
     2221 aacaagcagt ccgtcgacgc gaagatcagc gccatgaagc tccagatcac gctcgatgtg
     2281 aagcaggcgt ggctggcgct tatcaaggca cggcagacga tagagaccac aaatgtccag
     2341 atacggcagg cgacggagaa cctggagatc gcgaacctgc gctatgacgc cggactcgcg
     2401 acccctctcg aggtcacaga cgccacggtg acctacagcc aggcgaagct ggccaacata
     2461 agcgccctct tcgattacaa ggttgccagg gccaacatgg agaaggccat ggggagcaga
     2521 tgatggacgc acagaagggc aggggagtca tcggcaggct tcgggcgatg tcccggagga
     2581 aaaaggtcgt agcggcgggg atcgtcctcg tctgcgtggt cgtcatcgta tatgtcgcga
     2641 cgagaaagaa gccggcgccg ccgccggagc gggtggccgt gaccgcggcg aaggctgttg
     2701 cgaaggatat gcccgtggag ctgagggaaa tagggaccat cgaaggttat cgcagcgttc
     2761 ccatctacgc ccagataacg gggcaactgg tgaagaccca tttcaaagaa ggtcaggacg
     2821 tcaaaaaggg ccagcctctt ttcacgatcg atcccaacca gtaccaggca aaggtgcgcc
     2881 aggccgaggc gcagctggcg cgggacacgg cgcagatgaa attcgcccgc gacgaggcga
     2941 agcgttacaa gtacctctac gaaaagggcg cggtcgctct ttccgactac gagaacaagc
     3001 agtccgtcgc cgatgcgcag gagtccctcg ttgtcgcgga cagggcctct ttgcaggatg
     3061 cccggctcaa cctggcccac tgtttcatat ccgctccctt cgatgggcgc atgggcgcct
     3121 atgccgtctt tgaggggagg atgatcaagg acgtggatac ccagctagcg accatcaacc
     3181 agatatcgcc cgtctatgcc tccttttcca tagccgaaaa ggacctccct gccgtcagga
     3241 agtactccgt gggtcaaaag ctcaaagcga gggtgcttcc cgccgggtac acgggagagc
     3301 cgccggaagg cgagcttacc ttcatggaca ataccgtgaa cacccagacg gggatgatac
     3361 tccttaaggc gacatttccg aacaccgaca ggtttctctg gccgggacag ttcgtcaatg
     3421 tggtcctcac tctcaccacg gaaaagggcg tcgtcgtggt tcccgagcgg gctatacagc
     3481 tcagccagac agggaaatat gcctttgtca taaaggccga cgacacggtc gaatatcgcg
     3541 tggtcgcgac ggacaggact gtgcggggcc tgacggtggt gcgaaagggg ataaacgccg
     3601 gggagacagt cgtcaccgac ggccacttca agctgaggaa cggtttccct gtttcagtgc
     3661 gcgattcact cgcatcggga cagcggggta acggcggaag ccttgaggcc gctcccggaa
     3721 aacccgcgcc tcagaagacc ggcaccggag cgcccccgcc ggaagcctcc gcaggaacga
     3781 agaagtgaac atctcggcca tatggatcaa gcggcccatc atgaccatac tggtcatgtt
     3841 cggcattctc tttttcggca tcaacagcta cgtggacctt cccatcaata atctcccggc
     3901 cgtggatttc cctacgataa tggtttcggc cagccttccc ggggcaagcc ccgagaccat
     3961 cgcctcgaac gtggcaaagc ctctggagaa gcagttctcg accatacagg gcctgtcttc
     4021 catgtcctcc cagagctaca cggggtcgac tagcataatc cttcagttca ccctggaccg
     4081 caatatagac gcatgcgccc aggatgtgaa ctccaagata gcggccgccc agggggacct
     4141 gccgaccaac ctgccgagtc cccccacgta cgagaaggtc aacccctccg accagcccat
     4201 cctctatttt gcccttgttt cggagacgat gcccctcacc cagctcaacg attacgcgga
     4261 gaattatctt gcccagaatt tcagcatggt caacggcgtc tcccaggtca tcacctacgg
     4321 ccagaagttt gcggcgcgca tccaggtgaa ccccaagctt ctggcgaagc agggcatcgg
     4381 catcgacgac gtggagcggg ccatcaaggc ggcgaacgtc aaccttccgg gcggaaccat
     4441 cgacgggagc ttccaggcct tcacgatcga ttccaacgga cagttgacgg tcggagaggc
     4501 gtacagggat gtcatcataa cctataagga cggtacgccg gtccgcgtga aggacgttgg
     4561 agacgcgata agcggcgtcg agaaggacaa gcagatggcc gcctggtact cgacgcgcga
     4621 gaagacccgg cgcgccatcg tccttgccgt caagaaacag ccgggatcga acacggtcct
     4681 tgtcgctcaa ggcgtcaagg aactcgtccc ccgtctgaag tcgatggtgc ccgaatcggt
     4741 ggaatggcac ctcctctacg acgcctccga ttacattgaa gaatccatcc aggacgttca
     4801 gttcaccctt gttctgacca tcatcatcgt cctttttgtc gtcttcgttt tcctgcggtc
     4861 cttcaggtcg acggtcatac cgaacgtggc cgtgccgctg tccatcatcg gcacctttgc
     4921 ggtcatgagc gttctcggat tcagcctcaa caacctttcc atgatggcgc tggtcctgtg
     4981 cgtcggcctc gtcgtcgacg acgccatagt cgtcctcgag aacatagtgc ggcgcatgga
     5041 gatgggggag aacgccacgg aggcatcctt caaggggtct caggagatag ggtttaccat
     5101 catgtccatg accctgtccc tcgtggttgt ttttattccc ctc
//
LOCUS       DAOQTR010000083         5127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_17929_length_5127_cov_4.108044, whole genome shotgun sequence.
ACCESSION   DAOQTR010000083 DAOQTR010000000
VERSION     DAOQTR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17929_length_5127_cov_4.108044"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /gene="rplJ"
                     /locus_tag="PKN85_03050"
     CDS             <1..508
                     /gene="rplJ"
                     /locus_tag="PKN85_03050"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNT43409.1"
                     /translation="KTKVVEELGSKLKELDCLFLADFSGMNVAQVTKLRRELRGVGTE
                     FTVVKNTLLRIASKGTKAEALYDKFDGPNAIICTNGDPIGPAKVIAGLAKEIPNLKMK
                     AGYIGNRTITAEEIQRLATLPSRDVLLGKLVGLLKGQPARLVYALSGNLNKLMLVLNS
                     IKAQKEQA"
     gene            525..914
                     /gene="rplL"
                     /locus_tag="PKN85_03055"
     CDS             525..914
                     /gene="rplL"
                     /locus_tag="PKN85_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNT43410.1"
                     /translation="MSISREEVIQFIENMTVLELSEFVKELEDKFGVQAAMPMMAAAP
                     GSAAAAPAAEAAEEKTEFNVTLTGYEADKKIQVIKVVRAITGLGLKEAKDLVEGVPKA
                     VKEAVSKDEAASIKKQLEEVSGQVKVD"
     gene            1015..5109
                     /gene="rpoB"
                     /locus_tag="PKN85_03060"
     CDS             1015..5109
                     /gene="rpoB"
                     /locus_tag="PKN85_03060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT43411.1"
                     /translation="MKETFHNRIFRKNYGKIKEVLEIPNLIEIQLDSYEKFLQKDIAP
                     DKRDAIGLQAVFNSVFPIRDSHETTTLEFVKYDMGEPKNSEEECIVRGLTYAAPMKVT
                     IRLVVWNIDADTKARDIRAVKEQDVYFGEIPLMTERGTFIINGTERVIVSQLHRSPGV
                     YFDTDQSKTTVGGSLSYTARVIPYRGSWLDFEFDHKELVYVRIDKKKKIPVTLFLKAL
                     GYSEKEILDYFYEKETIVVKDRKLFKETPLALLVGQKAPSDIINEKTDEVLVKKHKKI
                     TKAVIKKMEQAGIHAIPVSEEDIIGKVTSGDIIDPKTKEVLIPINKELTAEDLESLVE
                     KKAKDFEILFIDNLNVSPSLRNTLNIDKVVTKEDALIEIYRKLRPGDPPTIEFAETLI
                     QNMFFSPERYDLSRVGRLKINHRLGIDVALDTTILRREDIIEVVRQLLKLKDARGPGD
                     DIDHLGNRRVRTVGELLENQFRMGLVRMERAIKERLTFPEMETLMPHDLVNPKPVATA
                     VKDFFASSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRIC
                     PIETPEGPNIGLIASLSTFAKVNEFGFIEAPYRQVVDKKVTDHVEYLSALQEEQYYIA
                     QANAPVDKEGKLLGELIPAQKGGSVFLVSPDQVEFMDVSPKQLVSASAALIPFLEHDD
                     ANRALMGSNMQRQAVPLLTTEAPIIGTGMERVVGRDSRVTIMARHDGVIESVDASRII
                     LKYEEKKGHDETATKIDVYKLLKFRRSNQDTCINQRVIVREGDFVKKGDVLADGPSTD
                     RGELALGKNVMVAFMPWRGYNYEDSVLINERLVKEDVFTSIHIEELECVVRDTKLGKE
                     EVTRDIPNVGDDALKDLDESGIVRIGATVKPGDILVGKVTPKGETQLTPEEKLLRAIF
                     GEKAGDVKDTSLRVPHGIEGIIIDVKVLSRRGIDKDDRSRDIEDKEISNILKDQEDQI
                     KIILDSKKAKIAELLEGKTAPADIKDGKGKALLKKGETFTREKVDSLSFDRLQEIQFG
                     DEELQSRITKLVESKENQVELIHIIYEDKINKIKKGDELPPGVIKTIKVYVAMKRKVA
                     VGDKISGRHGNKGIVSVILPEEDMPFTDDGTPIDIVLNPLGVPSRMNAGQILETHLGW
                     AAKEMGKRIGEMVDSYYRGLADSQAVKKLLKKTFSNNELKGVIDRLDDDEIASVAEAM
                     RKGVNIAVPVFDSAKEEEIREFFKMAGLIDSRQTTLFDGRTGDAFHHNITVGNMYFLK
                     LHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWASKGTEQPTPCRNSSP
                     SSPMTSMEGSGPMKRSSRARMSSNRICRNPSTFSSRNCRVSALT"
BASE COUNT         1342 a         1372 c         1467 g          946 t
ORIGIN      
        1 gaagacaaag gttgttgagg aactgggatc gaagctcaaa gagctcgact gccttttcct
       61 ggccgacttc agcggcatga acgtggccca ggtgacgaag ctcagaagag agctgcgcgg
      121 tgtcggcacg gaatttactg ttgtcaagaa caccctgctc agaattgcct cgaaagggac
      181 gaaggcagag gctctctatg acaagttcga tggtcccaac gcgatcatct gcacgaacgg
      241 tgacccgatc ggtccggcaa aagtcattgc cggccttgcc aaagagattc ccaacctgaa
      301 gatgaaagcg ggttacatcg ggaaccgcac catcacggcc gaggagatcc agagacttgc
      361 gacgcttccg tcgagagatg ttctgctcgg caaactcgtc ggccttctca agggtcagcc
      421 ggcacgactc gtttacgcgt tgtcggggaa tctgaacaag cttatgctcg tgcttaattc
      481 gatcaaggca caaaaagaac aggcataaag gaggatgtaa cataatgagt atatcacggg
      541 aagaagtgat ccagtttatc gagaatatga cagtgctcga actctccgag ttcgtcaagg
      601 aattggaaga caagttcggc gtccaggcgg cgatgcccat gatggccgcg gcaccgggct
      661 cggccgcggc tgctcccgca gcggaagccg cggaagagaa gaccgagttc aacgttaccc
      721 tgacgggata cgaagcggac aaaaagatcc aggtcatcaa ggtcgtccgc gccatcacgg
      781 gactcgggct caaggaagcc aaggatcttg tagaaggagt accgaaagcg gtgaaagaag
      841 ctgtctccaa agacgaagct gccagcatca agaagcagct tgaagaagtc agcggacagg
      901 ttaaggttga ttaaaaaaca attgtgcagg aaacaggggt ccggttatga agcggcaagc
      961 ctcatactgg atccctgtct tcagtttcaa cacccaaatg aggtgaaatg atctatgaaa
     1021 gaaaccttcc ataacaggat attccggaag aattacggaa agatcaagga ggttctcgag
     1081 ataccgaatc tcatcgagat ccagcttgat tcctacgaga agttcttgca gaaagacatc
     1141 gcgccggaca agagagacgc gataggcctg caggccgtgt tcaacagcgt cttccctata
     1201 agagactcac atgagacgac caccctggag ttcgtgaagt acgatatggg cgaacccaag
     1261 aactccgagg aggagtgcat tgtcaggggc cttacctatg cggcccccat gaaggtcacc
     1321 atccggctcg ttgtgtggaa tatcgatgcc gatacaaaag cgcgtgacat acgcgccgtc
     1381 aaggaacagg atgtctattt cggtgagata ccgctcatga cggagcgggg aacctttatc
     1441 ataaacggca cggaacgggt catcgtcagc cagcttcacc gttcgccggg agtttatttc
     1501 gacacggacc agagcaagac caccgtcggc ggaagtttgt cgtacacggc ccgcgtcata
     1561 ccctatcgcg gttcctggct cgatttcgag ttcgaccaca aggaactcgt gtatgtcagg
     1621 atcgacaaaa agaagaagat acccgtcacc ctcttcctaa aggcgctggg ttactcggag
     1681 aaggagatcc tcgactactt ctacgagaag gaaacgatcg tcgtgaagga caggaagctg
     1741 ttcaaggaaa cgcccctcgc gctgctcgta ggccagaagg ctccttcgga catcatcaat
     1801 gaaaagaccg acgaggtgct ggtcaagaaa cacaagaaga tcacgaaggc cgttatcaag
     1861 aagatggagc aggcaggtat ccatgcgatc cccgtgagcg aagaggacat catcggcaag
     1921 gttacctcgg gcgacatcat cgaccccaag acaaaagagg tcctcatacc cataaacaag
     1981 gagttgacgg cggaagacct ggagagcctt gtcgagaaga aggcaaagga cttcgagatc
     2041 cttttcatag ataacctcaa tgtgagcccc tctttgcgca acactctcaa tatcgacaag
     2101 gtggtcacga aggaagacgc cctcatcgag atataccgta aactgaggcc cggcgacccg
     2161 cccaccatcg agtttgccga gacgctgata cagaacatgt tcttcagtcc cgagagatac
     2221 gatctttccc gggtcggcag gctcaagatc aaccatcgtc ttggcatcga cgtcgccctc
     2281 gatacgacca tccttcgcag ggaggatatc atcgaggtcg tgcgccaact cctcaagctg
     2341 aaggatgccc gcggaccggg agatgatatc gaccacctcg gcaacaggag ggtccgcacc
     2401 gtgggcgaac tcctggagaa ccagttcaga atgggtcttg tgcgcatgga acgcgccatc
     2461 aaggagaggc tgacctttcc cgagatggaa actctcatgc cccacgacct tgtgaatccc
     2521 aagcccgtgg ccaccgcggt caaggacttt ttcgcctcga gccaactctc ccagttcatg
     2581 gaccagacga accctttgag cgagatcacc cacaagcgca ggctttcagc gttgggcccg
     2641 ggaggcctca cgcgtgaaag ggctggtttt gaggtgcgcg acgttcatcc cacccactat
     2701 ggaaggatat gccccatcga aacccctgag ggtcccaata tcggtcttat cgcctcgctg
     2761 tccaccttcg cgaaggtcaa cgagttcggt tttatcgaag cgccgtaccg ccaggtcgtg
     2821 gacaagaagg tcaccgacca cgtcgagtat ttgagcgccc ttcaggagga gcagtactat
     2881 atcgcgcagg ccaacgcgcc ggttgacaaa gagggcaagc ttttgggcga actgatcccc
     2941 gctcagaagg gcggcagtgt attcctcgtc agtcccgacc aggtggaatt catggatgtg
     3001 tccccgaagc agcttgtcag cgcctcggcg gcactcatac cctttctcga acatgacgat
     3061 gccaacaggg cgctcatggg ctccaacatg cagcgtcagg cggtgccact gctcaccacc
     3121 gaggccccca tcatcggaac cggcatggaa cgcgttgtcg gcagggactc gcgcgttacc
     3181 atcatggccc gtcacgacgg tgtcatcgag agtgtcgatg cgagccgcat cattctcaag
     3241 tatgaagaga agaaaggcca cgacgagacg gccacgaaga tagatgtcta caaacttctc
     3301 aagttccggc gctccaatca ggatacctgc atcaaccagc gcgtcatcgt cagggagggg
     3361 gatttcgtga agaaaggcga cgtcctcgcc gacggtccat cgacggacag gggtgaactc
     3421 gccctcggca agaatgtcat ggtcgccttc atgccctgga gagggtacaa ctacgaggac
     3481 tccgttctga tcaatgagcg gctggtaaag gaagacgttt tcacgtcgat ccacattgaa
     3541 gaactcgaat gcgtggtccg tgacacgaag ctgggcaagg aagaggtgac gagagacatt
     3601 cccaacgtcg gcgacgacgc ccttaaggac ctcgacgaga gcggtatcgt caggataggg
     3661 gcgaccgtca agccggggga tatacttgtc ggcaaggtga cacccaaggg cgagacccag
     3721 cttaccccgg aggagaaact gctcagggcc atcttcggtg agaaggccgg ggatgtgaag
     3781 gacacgagtc tcagggtccc ccacggtata gaaggcatca tcatcgacgt taaggtcctc
     3841 tcgaggaggg gcatcgacaa ggatgaccgc agccgggata tcgaggacaa ggagatatcg
     3901 aatatcctga aggaccagga agaccagatc aagatcatcc tcgacagcaa gaaggcaaag
     3961 atagcggagc tcctggaggg caagaccgct cccgccgata tcaaggacgg caagggcaag
     4021 gccctgctca agaaaggtga gaccttcacg agagagaagg tggattccct gagcttcgac
     4081 aggcttcagg agatacagtt cggagatgag gaactgcaat cccggataac gaagctggtg
     4141 gagagcaagg agaaccaggt cgagctcatc cacatcatat acgaggacaa gatcaacaag
     4201 ataaagaaag gcgacgagct gcctccgggc gtcatcaaga cgatcaaggt ctacgttgcc
     4261 atgaaacgga aggtcgccgt gggcgacaag atctcgggac gccacgggaa caagggtatc
     4321 gtttccgtca tccttcccga ggaagacatg cctttcaccg acgacggaac gcccatcgac
     4381 atcgttctta atcccctcgg tgttccctcc cgtatgaacg cgggccagat cctggagacc
     4441 catctggggt gggccgcgaa agagatgggt aagcgcatcg gcgaaatggt ggacagctac
     4501 taccggggcc ttgcagattc ccaggccgtc aagaaactcc tcaagaagac cttttccaat
     4561 aacgagctca agggtgttat cgaccgtctt gacgacgatg agatcgcgtc cgtagcggag
     4621 gcgatgagaa agggcgtgaa tattgccgtt cctgtcttcg acagcgccaa ggaagaggag
     4681 atcagggaat tcttcaagat ggcgggcctc atcgactcac gtcaaacgac actgtttgac
     4741 ggcaggaccg gcgatgcctt tcaccataac ataaccgtcg gtaacatgta ctttttgaag
     4801 ctccaccatc tcgtcgacga caagatacat gcccggtcga tcggtcctta ctcgcttgtg
     4861 acccaacagc ccctgggcgg caaggcccag ttcggcggcc agcggctggg tgagatggag
     4921 gtttgggcct cgaagggtac ggagcagcct actccctgca ggaattcctc accatcaagt
     4981 ccgatgacgt caatggaagg atcagggcct atgaagcgat cgtcaagggc gaggatgtcc
     5041 tcgaaccgaa tttgccggaa tccttcaacg ttctcgtcaa ggaactgcag ggtctctgca
     5101 ttgacgtaga gctccttaaa gaagagt
//
LOCUS       DAOQTR010000084         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_18033_length_5107_cov_3.782660, whole genome shotgun sequence.
ACCESSION   DAOQTR010000084 DAOQTR010000000
VERSION     DAOQTR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18033_length_5107_cov_3.782660"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="ispG"
                     /locus_tag="PKN85_03065"
                     /gene_synonym="gcpE"
     CDS             complement(<1..811)
                     /gene="ispG"
                     /locus_tag="PKN85_03065"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNT43412.1"
                     /translation="MKRKKTRQMHVGGIPIGGGSPIVVQSMLKTNPENFIETLEQTRE
                     LKKAGCELVRIALPQEETCKIIPFLRKEVDVPIIGDIHFNHKIALKAMELGIDGIRVN
                     PGTINNLRKMREIATAALETKTPVRLGMNIGSMEKRVLKKYTRPSADAMVESALYHVA
                     LFEDVGWTELKVSLKASDIRETIDAYRKFSRKSDYPLHVGITEAGPVFSGAIKSAIGI
                     GILLHEGIGDTIRVSLTGNPVYEVIAAYHILRDLGLRKRGINIISCPTCGRT"
     gene            939..1286
                     /locus_tag="PKN85_03070"
     CDS             939..1286
                     /locus_tag="PKN85_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43413.1"
                     /translation="MNDGKKKGIILIIIGICIPLFALPFVSGLDINKGFMDNFYNAGI
                     RITKSAVPAPPETAAGKPSGDTAGKNPLSLERMRIERIPFRFFLVPTFILLYIGIVMI
                     DRAKSREKKTGHV"
     gene            1680..2822
                     /locus_tag="PKN85_03075"
     CDS             1680..2822
                     /locus_tag="PKN85_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNT43414.1"
                     /translation="MFADSVMSGLNGLTAGFANLHWSHPVMMIIGGLLLYLGIKKDCE
                     PLLLVPIGFGCILINIPLAGLMDKEGFLRIIYDAGVITELFPLFIFVGIGAMTDFGPV
                     LENPYTFLLGAAGQFGIFITLLLALALGFPYKDAVTIGIIGACDGPTVIYVASQYAQH
                     LLGPVTVAAYSYMALVPVLQPPIMRMLTTQKERTTVMKSHAKSVSKTTKMLFPIVITI
                     VGGLIAPKGLPLLATIMLGNLMKESGVVSRLTSAAENEIANIVTLLLGISIGATMSGP
                     VFVQPKTLIILILGLLAICLDTVCGVLFGKVLYVVTGGKVNPLIGASGISAFPMAARV
                     AQKEGLKYNKRNYLLMHAMGANAGGQVGSVMAAAVMLSVLNGMGLI"
     gene            3108..4439
                     /locus_tag="PKN85_03080"
     CDS             3108..4439
                     /locus_tag="PKN85_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNT43415.1"
                     /translation="MDFVQTASLVIFLASIILVITGWIDSVLAALLGILFMVFFGIMS
                     DIDAFKIVDWNVIIILLSIWIISGYFGKSGVPDFLSAAILKLSKGNVALFVTMIGALA
                     GFVSMLIDNVVVVLMFAPVIFHACRRFNFKAFGPVLFLALCANFMGTAMLLGDLPPQM
                     LHSVSGIEFSGFIWQLGRPSSLFILLISYVVTCWMFYMMFKRSYKGMTMDVASMDERP
                     LDHIKDKPFAIVVCIVFALTIVAMALRPIFGYHLGFIAMWGAICLILIFEILKDRFTL
                     EIPSLEHVLSELDWRAVFFYVALFALVGGLEHSGVIKLVSNAITPLIKQSLVMGSTLL
                     FWVTAPIVGIVEHDAYILTMLYVIRDLAKDTGMNPWPLYWMLLWAGTLGSNLTIAGAP
                     ALFVAKNLAEKEDQEKISLKSFLGMTVPYVIVSLMACYIPAMLIWVFPFAK"
     gene            4451..>5107
                     /locus_tag="PKN85_03085"
     CDS             4451..>5107
                     /locus_tag="PKN85_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNT43416.1"
                     /translation="MAILTISRQFGGGARAIALQVARDLGYEYVDRSTMFKDISSEGP
                     RWGTYAKDFDEHYPNVWERNDWSFKAFVALIQNLMYGYAQRDKVIIAGTGATFLLKGV
                     PHVLRVRIESSMEDRINRVQGDQVINESTARWIIDKVDADMSRSMYVIYGKEWDDPKE
                     FDSVFNSSTQTQDEIVEEIKKGLAAKEALRTKEALDILNLRAIASKVKAAILTDPSFT
                     V"
BASE COUNT         1035 a         1434 c         1382 g         1256 t
ORIGIN      
        1 tcgtcctgcc gcaggtgggg caggagatga tgttgatgcc tcgtttcctc aggcccaggt
       61 cgcgcaggat gtgataggcg gctatcacct cgtacacggg attgccggtc agcgagaccc
      121 tgatggtgtc tcctatgccc tcgtggagca gtatgccgat gcctatcgcc gacttgatgg
      181 ctccggaaaa gacggggccc gcctccgtta tccccacgtg aaggggataa tcggacttcc
      241 tggagaactt cctgtaagcg tcgatggtct ccctgatgtc cgatgccttg agagagacct
      301 tgagctccgt ccatcccaca tcctcaaaga gggccacgtg gtacagggcg ctttcgacca
      361 tggcgtccgc gcttggcctc gtatacttct tgaggaccct tttctccatg gaaccgatgt
      421 tcatcccgag tctcacgggc gtcttcgttt ccagggccgc cgtcgcgatc tcccgcatct
      481 tgcgcaggtt gttgatggtg ccggggttga cccttatgcc gtcgatgccg agttccatgg
      541 ccttcagcgc tatcttgtgg ttgaagtgaa tgtcgccgat gatgggtacg tccacttcct
      601 ttctgaggaa ggggattatc ttgcacgttt cttcctgggg cagggcgatc ctgacgagtt
      661 cgcacccggc cttcttaagt tcccgcgtct gttccagggt ctctatgaaa ttctcgggat
      721 tggtcttgag catggattga acgacgatgg ggctgccccc gcctatgggg atacccccga
      781 cgtgcatctg tcttgtcttt ttacgtttca tcccctgttc cttgaattga agtggctgaa
      841 tcttgcttat catatcccca ccgggacccg atgtcaatca tcagcgtagg caaaaaagtg
      901 tccttctgcc gtccgaccta tggtatagtg aaccaatcat gaatgacggc aagaaaaaag
      961 gcatcattct catcatcatt ggtatctgca ttcccctctt tgccctgccc tttgtctccg
     1021 gtttggacat caacaaaggg tttatggata atttctacaa cgcgggcatc aggataacga
     1081 agagtgccgt tcccgcaccg cctgaaaccg ccgccgggaa gccgtcggga gacaccgcgg
     1141 ggaagaaccc tctttctctt gagagaatga ggatcgaaag gataccgttc aggttctttc
     1201 ttgttcccac cttcatcctt ttatacatag ggatagtcat gatcgatagg gcaaaatcaa
     1261 gggagaagaa gaccgggcac gtgtagtgtc tgttcccgct cgtattccgt tgtcctttcc
     1321 tccccccccc ctttttttta aggtgtcggc gtggttcttc cggattcccc gagagcggac
     1381 aatatggcac cttccttttg ctcaacaatg cgcctcctcg ttaacaattt cacttgacta
     1441 taggtaagtg gttgagatag tgttttacag caatttcggc acctttaatt attcacataa
     1501 aaaccaccaa accgggagga cgggttacta tggacaaatg gacctttggc ttcaccatgg
     1561 ttgttgtcgg gatgggcggt accatatgta ccctcattgt tttcagtctc attatgtctg
     1621 ctctcaaaag gatctttccc tacaggaaag aagaagacaa ataaagggag gattggggca
     1681 tgtttgctga ttcggtaatg agcggtttga atggacttac ggctggtttc gcgaacctgc
     1741 actggtcgca tcccgtgatg atgatcatag gtgggttgtt gctgtacctg gggatcaaga
     1801 aagattgcga gcccctgctt ctggttccca tcgggttcgg atgcatcctc atcaacatac
     1861 ccctcgctgg actgatggac aaagagggat ttttgcgcat catctacgat gccggtgtca
     1921 tcacggaact ctttcccctt ttcatattcg tcggcatagg ggcgatgacg gatttcggac
     1981 ccgtcctgga gaacccttat accttcctcc tgggtgcggc aggtcagttc ggcatattca
     2041 tcaccctcct tctcgccctt gctctcggct ttccttacaa ggatgccgtg acaatcggca
     2101 tcataggagc ctgtgacggt cccaccgtca tttacgtggc ctcgcagtac gctcaacacc
     2161 tgttagggcc cgtgacagtg gcggcctatt cctacatggc cctcgtcccg gtcctccagc
     2221 ctcccatcat gaggatgctg acgacgcaga aggaacgtac cacggtcatg aagagccacg
     2281 caaaaagcgt gtccaagacg acgaagatgc tctttcccat cgtcatcacc atcgtcggcg
     2341 gtcttatcgc ccccaagggc ctccctcttc tggcgacgat catgctcggc aacctcatga
     2401 aagaatcggg ggtcgtcagc aggttgacga gtgcggcgga aaacgagata gccaatatcg
     2461 tgacactcct cctgggcata tccatcggcg ccaccatgag cgggccggtc tttgtgcagc
     2521 ccaagaccct catcatcctt atcctcgggc ttctcgccat atgtctcgac acggtgtgcg
     2581 gcgtcctttt tggaaaggtc ctctacgttg ttaccggagg gaaggtcaac cccctcatcg
     2641 gtgcctcggg catatcggcc ttccccatgg cggcccgtgt cgcccagaag gaaggattga
     2701 aatataacaa gagaaattat cttctcatgc atgccatggg agccaatgcc ggcgggcagg
     2761 ttgggtcggt catggccgcc gccgtcatgc tctccgtcct caacggaatg ggtctcatct
     2821 agtaagaata acagggaacg gaatggaaag gggccggaca gtcaacgatc tgtccggccc
     2881 ccccattttt taggtatccc tggaaagagg aaaaggagtc ggggggctcg ccccgcaact
     2941 cgacccttcc attcgatagt gcttgcttca aagaagtgac ttgttatata gtagcttcag
     3001 tgtccatcaa aactagaatc gtgcagacct tcagctagtc ggggtgacta aagagtgtac
     3061 gctcttggtc tgcttggtga tgcacagaaa aagggggttc agagtctatg gatttcgtgc
     3121 agaccgcatc acttgtgatc tttctcgcca gcatcatcct cgtcataaca ggttggatcg
     3181 acagcgttct ggccgcgttg ctgggtatcc tcttcatggt cttcttcggg atcatgtcgg
     3241 acatcgatgc cttcaagatc gtcgactgga acgtcatcat catcctcctc agcatctgga
     3301 tcatctcggg gtatttcggg aagtcggggg tgcccgattt cctttcggca gccattctca
     3361 agctgtcgaa gggcaacgtg gccctcttcg tgaccatgat cggggcactg gccggattcg
     3421 tctccatgct catcgacaac gtggtcgtcg tcctcatgtt cgcgcccgtc atctttcacg
     3481 cctgccggcg gttcaatttc aaagccttcg gtcccgtgct ctttctggcg ctttgcgcga
     3541 acttcatggg tacggcgatg cttctcggcg acctgccgcc gcagatgctt cacagcgtgt
     3601 ccgggatcga gttcagcggt ttcatctggc agctgggcag gccgtcgtca ttgttcatcc
     3661 ttctcatttc ctatgttgtg acctgctgga tgttctacat gatgttcaaa aggtcctaca
     3721 aagggatgac catggacgtg gcgtcaatgg acgagcgtcc cctggaccac atcaaggaca
     3781 agccttttgc catcgtggta tgcatcgtct tcgccctgac catcgtggcc atggcccttc
     3841 gtccgatctt tggctaccac ctcggtttca tcgcgatgtg gggcgccatc tgtctcatcc
     3901 tcatcttcga gatcctcaag gaccgtttca cgctggagat ccccagcctc gagcacgtgc
     3961 tttcggaact ggactggcgt gccgtgttct tctatgtcgc gctcttcgct ctcgtcggcg
     4021 ggctcgagca ttcgggggtc atcaagctgg tgtcgaacgc gatcactccc ctcatcaaac
     4081 agagcctcgt gatgggaagc acgctccttt tctgggttac cgcccccatc gttggcatcg
     4141 tggagcacga tgcctatatt cttaccatgc tctatgttat ccgcgacctt gccaaggata
     4201 cgggtatgaa cccctggccc ctctactgga tgctcctgtg ggccggtact cttggcagca
     4261 acctgacgat cgccggagct ccggcgctct tcgtggcgaa gaacctggcg gaaaaggagg
     4321 accaggaaaa gatatctctg aaatccttcc tcgggatgac ggttccctat gttatcgtgt
     4381 cgctgatggc ctgctacata ccggccatgc ttatctgggt cttcccattc gcgaaatagg
     4441 gggtatggtc atggcaatat tgacaatttc acggcagttc ggaggcggag cgagggcgat
     4501 cgcgctccag gtcgcccggg acctggggta cgaatacgtc gaccgctcca ccatgttcaa
     4561 ggacatctcg tcggagggtc cgcgatgggg tacgtacgcg aaggacttcg acgagcatta
     4621 cccgaacgtg tgggagcgca acgactggtc gttcaaggcc ttcgtcgccc tcatccagaa
     4681 cctcatgtac ggctacgcgc aaagggacaa ggtgatcatc gccggcacgg gcgccacctt
     4741 tctgctgaaa ggggtccctc acgtcctcag ggtccggata gagagctcca tggaggaccg
     4801 cataaacagg gtgcagggag accaggtgat caacgagagc accgcccggt ggatcatcga
     4861 caaggtcgac gccgacatgt cccgttccat gtacgtcatc tacggaaagg aatgggatga
     4921 cccgaaagag ttcgacagcg tgttcaacag ctcgacacag acgcaggatg agatcgtgga
     4981 agagatcaaa aagggccttg cggcgaagga agccctgaga acgaaggaag ccctggacat
     5041 cctgaacctc cgggcgattg cgtcgaaggt aaaagccgcg atcctgacgg acccctcctt
     5101 taccgtt
//
LOCUS       DAOQTR010000085         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_18145_length_5086_cov_3.952296, whole genome shotgun sequence.
ACCESSION   DAOQTR010000085 DAOQTR010000000
VERSION     DAOQTR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18145_length_5086_cov_3.952296"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKN85_03090"
     CDS             <1..400
                     /locus_tag="PKN85_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNT43417.1"
                     /translation="LARDATGDHPSVYMNLIRLKGPRRVDDMCAYPWGEGELLISGAS
                     GDEEALFEVSSADIILFRRHPEAISARNLLQCRVSELFNTGGRIGVALDCGGERLVAL
                     VVRRAVEDLEIREGVEVYAAIKATAFRQLG"
     gene            complement(413..763)
                     /locus_tag="PKN85_03095"
     CDS             complement(413..763)
                     /locus_tag="PKN85_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HNT43418.1"
                     /translation="MNIQIYGTSKCQDTRKAQRFFKERGIPFQYIDLTVKGLSRGELN
                     SVKAAVGIENLMDTQGQRYVRRNLTYLTHDPEEELLGDPLLFKTPIVRNGAKATVGYA
                     PEVWKGWKGKEDRP"
     gene            complement(780..2600)
                     /gene="typA"
                     /locus_tag="PKN85_03100"
     CDS             complement(780..2600)
                     /gene="typA"
                     /locus_tag="PKN85_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNT43419.1"
                     /translation="MEQNKIRNIAIIAHVDHGKTTLVDQFFRQSGMFRHNEIVEERLM
                     DSMDLERERGITISSKNGSFAYKDFFINIIDTPGHADFGGQVERVLKMADGVLLLVDA
                     AEGPMPQTRFVLKKALNLNLPVIVVVNKIDKPAARCDWVVDQVFDLFVKLDAPDDILD
                     FPIIYTSAKAGYATLDHTQAGGSMLPLFDMITDSVPPPMGNPDAPLQMLVSSLSYSPF
                     LGRLAIGKITSGVLNLNKDVIVATEGVPSAPARITKVYRFKGSGMEETEIAGTGEIVA
                     VAGMDSITIGETLTDPENPAPLPGIDIDPPTISMNFIPNDSPFAGKEGRFLTSRHIRE
                     RLMRETLSDVALVVEELSDSIGYKVSGRGELHLSILIEKMRREGYEFQVTRPKVIFKD
                     SDDGVLEPYEELTIDVDEEYSGNVIENLGRRKGQMVHMHQDKGMVRLSYRVPTRGLIG
                     FRSQFLTETKGTGTMNYIFDGYDRYAGEMKNRNNGVLVVMEECTTVAYALFNLQERGE
                     LFLGPGEKVYGGQIIGEHTREIDLVINPAKGKKLTNMRAAGSDDAVILTPPRYMSLED
                     CISYINEDELVEITPRSIRLRKTILDGLQRKRSKIAGQKA"
     gene            complement(2654..2854)
                     /locus_tag="PKN85_03105"
     CDS             complement(2654..2854)
                     /locus_tag="PKN85_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNT43420.1"
                     /translation="MAKGTVKWFNESKGFGFITGDDGTDVFVHYSAIQGSGFKSLSEG
                     EQVTFDVVNGPKGPAASNVAKA"
     gene            3152..3943
                     /locus_tag="PKN85_03110"
     CDS             3152..3943
                     /locus_tag="PKN85_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT43421.1"
                     /translation="MTDLDRGLRSYDRPEILYRLFFPRRDVAPDSESPRAFNRFVEVA
                     EGVSICCRCYPARPDGPNILYFHGNGEVAADYDHVAPVYAERGLNLFVADYRGYGGSG
                     GRPTCTAMIKDAHPVFRDFMEYIGKNGLTGSPFVMGRSLGSAPAIELAYHYQAEFEGL
                     IVESGFARAANQVARLGVEHLFDDEDPVGFGNDLKIADVKIPTLIIHGEWDQIIPVSE
                     GKTLYELSGAAGKSSLFIPRAGHNDIMMLGLKAYMDAVQAFTRRA"
     gene            4062..4889
                     /locus_tag="PKN85_03115"
     CDS             4062..4889
                     /locus_tag="PKN85_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNT43422.1"
                     /translation="MAHKPVEIVQLAGDAGKYKANLTPGNYLVRSFMAGLFIAVGGAL
                     ATVCSTGLENSIGPGFKSMIAGSVFPVGLIAIVLTGMSLFTGDTMLIPMAVFQNKSTW
                     GKVINAWFWVYIGNFIGSLFWAYLMTVGPFSKGGGAELTAFGQSAVAIAQAKTLPYMA
                     NGGAGLWSAFMKGIGCNLLVNLAILLALSSKNMIGKFFGIWFPIMAFVSSGFEHCVAN
                     MYFIPAGIMLGANVTWGQFIGWNLIPVTIGNIVGGFVFIGAVYFISFKKELSTITPT"
BASE COUNT          995 a         1391 c         1529 g         1171 t
ORIGIN      
        1 gttggcgcgc gatgccaccg gggaccatcc gtccgtctac atgaacctga tacggctcaa
       61 agggccccgg cgtgtcgacg acatgtgcgc gtacccgtgg ggagagggcg aactgcttat
      121 ctctggagca agcggagacg aggaggccct ttttgaggtc tcttcggcgg acatcatact
      181 cttcaggcgt cacccggagg ccatcagcgc gcgcaacctc ctgcagtgcc gggtatcgga
      241 gttgttcaat accggcggga ggatcggcgt tgcgttggat tgtggaggcg aaaggcttgt
      301 cgccctggtg gtccgaagag cggtcgaaga cctggagatc agggaaggag tggaggttta
      361 cgccgccatc aaggcaaccg cctttcgaca gctgggttag gccttacgtg catcacggac
      421 gatcttcttt tcccttccat cccttccaga cttccggcgc gtagcccacg gtcgcctttg
      481 caccgttgcg gacgatgggt gttttgaaga ggagggggtc gccgagcagc tcttcctccg
      541 ggtcatgggt gagatacgtg aggtttcgcc ggacgtagcg ttgcccctgc gtgtccatga
      601 ggttctcgat gcccacggcg gccttgacac tgttgagctc acccctgctc aggcccttga
      661 ccgtcaggtc gatgtactga aatgggatac cccgctcctt gaagaaccgt tgggcttttc
      721 gtgtgtcctg gcatttggag gtgccgtata tctggatgtt catggcgcga cggggtttgt
      781 caggccttct ggccggctat tttcgatctc ttgcgctgga gcccgtcgag gatggtcttg
      841 cgcagtctga tggacctcgg tgtgatctcc acgagctcat cttcgttgat atatgagata
      901 cagtcttcca ggctcatgta ccggggaggc gtgaggatga cggcgtcgtc ggagccggcc
      961 gccctcatgt tcgtcagttt cttgcccttg gcggggttta tcacgaggtc aatctcccgc
     1021 gtgtgttcac cgatgatctg gccgccgtag accttttcac cgggaccgag aaagagttcc
     1081 ccgcgctcct gcaggttgaa aagggcgtag gccacggtgg tgcattcctc catgaccacg
     1141 aggaccccgt tgttgcggtt cttcatctcg ccggcatacc ggtcgtagcc gtcgaagatg
     1201 tagttcatgg tgcccgttcc cttggtctcc gtcaggaact gggagcggaa accgatgagg
     1261 ccccgcgtgg ggacgcggta gctcagcctc accatgccct tatcctggtg catgtggacc
     1321 atctggcctt tgcgccgccc gagattctct atgacattgc cggagtactc ctcgtcgacg
     1381 tcgatggtca gttcctcgta gggttcaagg accccgtcat cactgtcctt gaatatgacc
     1441 ttcgggcggg tgacctggaa ttcgtaaccc tccctgcgca tcttctcgat gaggatggac
     1501 aggtgaagct cgccccggcc cgacaccttg tacccgatgg agtcactgag ttcctcgacg
     1561 acgagggcca cgtcggagag tgtctctcgc atgagcctct ccctgatgtg ccgcgacgtg
     1621 aggaaccggc cttcctttcc ggcgaaaggg gaatcgttgg gaatgaagtt catcgatatt
     1681 gtcgggggat cgatatcgat gccgggaagg ggcgccgggt tctcagggtc cgtcagcgtt
     1741 tctccgatgg ttatggaatc catccccgcg acggcgacta tctcaccggt gccggctatc
     1801 tccgtctctt ccatgccgct tcccttgaaa cggtagacct tcgttatccg ggccggtgcc
     1861 gagggtaccc cttccgtggc tactatcaca tccttgttga ggttgagaac gcccgaggtg
     1921 atcttcccga tcgccagcct tcccagaaag ggtgaataag agagggagct cacaagcatt
     1981 tgaaggggag cgtcggggtt tcccatgggg ggaggcacgg aatcggttat catgtcgaag
     2041 aggggcagca tcgaaccgcc cgcctgcgtg tggtcgagcg tggcgtagcc ggccttcgcc
     2101 gatgtgtaga tgatcgggaa atccagtatg tcgtcgggcg cgtcgagctt gacgaagagg
     2161 tcgaagacct ggtccacgac ccagtcgcac cgcgcggccg gcttatcgat cttgttgacg
     2221 acgacgatga cgggcaggtt gaggttgagg gccttcttca ggacgaacct cgtctgcggc
     2281 atcggtcctt cagcggcatc gacaagcagg aggactccat cggccatctt gaggacccgc
     2341 tccacctggc caccgaaatc ggcatgcccc ggggtgtcga tgatgttgat gaagaagtcc
     2401 ttgtacgcga aggagccgtt ctttgaagag atggtgatac cccgttcccg ttcgaggtcc
     2461 atggagtcca tgagcctctc ctcgacgatc tcgttgtggc ggaacatgcc gctttgtctg
     2521 aagaattggt ccacaagggt ggtcttcccg tggtcgacgt gggcaatgat cgcaatattc
     2581 ctgatcttat tctgttccat cggggtgggg atcgaaggca ggcccgactg tcgccgggcc
     2641 tgcatcgggt ggtttacgct tttgcgacgt tggatgcggc cgggcctttg gggccgttga
     2701 ccacatcaaa ggtaacctgc tcgccttcgc tcagggactt gaaaccgctg ccctgaatgg
     2761 ctgagtaatg cacgaacacg tcggtgccgt catcgcccgt aatgaaacca aaaccttttg
     2821 actcgttgaa ccatttaaca gttccctttg ccatgctaca aacctcctaa aggatattga
     2881 aatgtggaag aaaaggaact gtgctatggg acagacaaca aaaatgggaa attcctgcgt
     2941 atgtgacgaa cagcgtgttc cttcctatgc tccggggtcg attatctcac aaaaataagg
     3001 ggagtgcaac aaatattctc gttggggccg aaaaatatgg cacaagcggg ttttcttcct
     3061 tgatatgaga tgacgaattc ctttaaccta agaagggggc acggcgcgac cggcattctg
     3121 ggatggtccg ggtcctgaag ggaggcgcgg catgacagat cttgacagag ggcttcgatc
     3181 ctatgaccgg ccggagatcc tctaccggct tttctttcca aggcgcgatg tggcgcccga
     3241 ttcggagagt ccgagagcgt tcaacaggtt cgtggaggtg gcggagggcg tttcgatctg
     3301 ctgccgctgc tatccggcac ggccggacgg gccgaacatt ctctattttc acggcaacgg
     3361 agaggttgcc gccgactatg accacgtggc gcccgtctat gctgagcggg gattgaatct
     3421 tttcgtcgcc gattaccggg gatatggggg aagcggcgga cgtccaacgt gtacggccat
     3481 gataaaggat gcccaccccg tcttccgtga cttcatggag tatatcggca agaacggcct
     3541 cacgggaagc cccttcgtta tgggcaggtc tctcggaagc gccccggcca tcgagcttgc
     3601 ctatcattac caggcggagt tcgaaggtct catcgtggag agcggttttg cgagggccgc
     3661 gaaccaggtg gcaagactgg gagtggagca cctcttcgat gacgaggacc ctgtcgggtt
     3721 cggcaatgac ctgaagatag cagacgtgaa gatccccacg ctcatcatcc acggcgaatg
     3781 ggaccagatc atccccgttt cggagggaaa gacgttgtac gaactgtcgg gagctgccgg
     3841 gaagtcctct ctctttattc cccgggccgg tcacaacgac atcatgatgc tcgggctcaa
     3901 ggcttacatg gatgccgttc aggcattcac ccgaagggcc tgataaattg cgagaattaa
     3961 ttacttgata tctgcccgct ttcagtggtt ttcttaagaa aatcacaatc ggcacccagc
     4021 agcaagacag aaggttgaga caaccccaaa aaggagggat tatggcgcac aaacctgtgg
     4081 agatcgtgca attggctgga gatgcaggca agtacaaggc taatctcaca cctggcaatt
     4141 atcttgtacg gagctttatg gcaggcctct tcatcgctgt cggaggggcc cttgccacgg
     4201 tatgttcgac gggacttgag aactccatag gtccggggtt caagtcaatg atagcgggtt
     4261 cggtcttccc tgtgggcttg atagccatcg tcctcacggg catgtcgctt ttcaccggcg
     4321 acacgatgct gatccccatg gcagtgttcc agaacaagtc gacctgggga aaggttatca
     4381 atgcctggtt ctgggtttac ataggtaatt tcatcggctc tcttttctgg gcgtatctca
     4441 tgacggtagg gcccttcagc aagggcggcg gcgccgagtt gaccgcgttc ggccagagcg
     4501 ccgtggctat cgcgcaagcc aagacgctgc cctacatggc gaacggcggg gcgggtctgt
     4561 ggtcggcctt catgaagggt atcggatgta accttctcgt caaccttgcg atccttctgg
     4621 cgctttcatc caagaacatg atcggcaagt tcttcgggat atggttcccg atcatggcct
     4681 tcgtttcgtc cggcttcgaa cactgcgtgg ccaatatgta tttcatcccc gcaggtatca
     4741 tgctgggcgc gaatgtcacg tgggggcaat tcatcggatg gaatctcatc cccgtcacga
     4801 tagggaacat cgtcggtggt tttgtgttca tcggtgctgt atatttcatc tctttcaaga
     4861 aagagctttc aaccatcaca ccaacctgat acgaagcaat gcaccccgga gcgcgaacgc
     4921 tccggggtgc attcaagacc gtctacccat cgtatcaact gcagaggcgt gcttcggctg
     4981 cctccaaggt ttccatcttc ttcgcgaaag cgaagcgaac cgtcttcttg cccatgccct
     5041 tcgtgccgta gaaacacgac ccggggacaa cggcgactcc caggtt
//
LOCUS       DAOQTR010000086         5082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_18174_length_5082_cov_3.386712, whole genome shotgun sequence.
ACCESSION   DAOQTR010000086 DAOQTR010000000
VERSION     DAOQTR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18174_length_5082_cov_3.386712"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PKN85_03120"
     CDS             <1..751
                     /locus_tag="PKN85_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNT43423.1"
                     /translation="VRLRAFGMFNVRVIQPSLFINTLVGTKASYTVEDIEDYLGKVIL
                     SRFNDYLGSNLDTLLDLPGQYDTMSAGLTQMLEKDFSHFGIQLSGLYINSITPPQEVQ
                     KAIDDKSRLAMFDDLNKLVKMKAAMAMESAAQNPGEAGAGMGMGLGFMMPAMYAETLK
                     GAPRSDGGMKCPECQNPINEDARFCPSCGHHLLVISQCPKCHKNLPARARFCPACGQS
                     VTAREELLKCPHCGTDNLSGSVFCNHCGEKI"
     gene            757..2073
                     /locus_tag="PKN85_03125"
     CDS             757..2073
                     /locus_tag="PKN85_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43424.1"
                     /translation="MQIEHQCPQCGAPVVLEETDRLLTCDFCKTRLYMQPRDYFRYCF
                     TPLDPFLEQVIYVPYWRFRGVHFSCRTSGITNGILDKTFLAVESKHFAQTLGLRPQAL
                     KLRFVQPADNARFIPPNIPFEKSFVEAKHNVTYETVTVHETRFVRRGTEGDYAEILVT
                     RKELKEERLYHEAFVADTMSLIYTPMFARNGRFHDAVLNEALYEGPPVYESFPYEFFS
                     GGWAINFLPTLCPECGWNTLAERDSCVLLCNGCNSAWRMDGQGLGRVGFATALSTKAA
                     PGSVRQYLPFWRLRVEVEGLKLDCYADLVRLGNLPRVIQPGWEEPPFFFWTPAFRMTP
                     AVYRRMSKQFTIANFSGEFHDRLPDAPVQAVNLTPQDALDSLITIIADFAINKKKVLP
                     LLPDMRITLREALLVLFPVTETDHEYIQPDINCGLMKNVLEQGKNI"
     gene            complement(2101..3189)
                     /gene="ychF"
                     /locus_tag="PKN85_03130"
     CDS             complement(2101..3189)
                     /gene="ychF"
                     /locus_tag="PKN85_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNT43425.1"
                     /translation="MSFSCGFIGLPNSGKSTIFNALTALAVPCQPYPFCTIDPNVGVV
                     PVPDARLFSLARLLKPEKITQTTIEFVDIAGLIKDAHKGEGLGNRFLSHIRNVDAVAH
                     VIRAFNSGQVPHVYADIDPARDMDIVETEIVISDLEIVEDRIRKIERLAKISKDKGEA
                     EMELLLRVRRYLEAGRFVATDDFDENERQALRAFDLITTKPLFYIANVDEEGLSASPQ
                     PAIEEAAAKRGRQVVYICGKLEQDLSALPEDERASFMELYDMKEFSIEKIIHVGYRIL
                     GLITFYTVVGKEMRAWTIPRGTTCAKAAGKIHTDMEKGFIRAEVINIDDLITCGSEHA
                     AREKGLLRLEGRDYLVEDGDILHIRFNV"
     gene            complement(3200..3757)
                     /gene="pth"
                     /locus_tag="PKN85_03135"
     CDS             complement(3200..3757)
                     /gene="pth"
                     /locus_tag="PKN85_03135"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNT43426.1"
                     /translation="MHLLCGLGNKGSEYAFTRHNVGYLVIDRFSERFKIPVRKKDSGC
                     RIGSKDDLVLAKPDTYMNLSGGPIARLAGKLKVPPDRVIVIHDDMDMDFGKMRIRLGG
                     RDGGHKGVRSVIECLGGKDFWRLKIGIGRNPSMPAEEYVLSRFDGDEAGELADIIDIA
                     CDALKVFVFEGGSKAMSLFNRRGPE"
     gene            complement(3764..4393)
                     /locus_tag="PKN85_03140"
     CDS             complement(3764..4393)
                     /locus_tag="PKN85_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNT43427.1"
                     /translation="MEQVLIKADRRSATGKGVARKARAAGRIPAVLYGGGVDPVSITI
                     SSKDWDKITRHMKRNVILDMEIHGADSVESRPVMVKEVQRNGLGTEIMHIDFLQVSME
                     KTVEVEVSIHLKGKSKGEVLGGVIDVHLRSIKVECLPGQIPEEIVIDMTELDIGDSVH
                     VSDISLPGVKLIEHGDVAILSVVPPTVEEKRVATEEAAAEASEAKEKEE"
     gene            complement(4408..>5082)
                     /locus_tag="PKN85_03145"
     CDS             complement(4408..>5082)
                     /locus_tag="PKN85_03145"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNT43428.1"
                     /translation="VIPYYGYARQDRKVLPRTSISARLVANLITVAGASRILAMDLHA
                     GQIQGFFDIPVDHLYALPVQFEYIKKIKGDIVVVSPDAGGVERARELGKRINATIAII
                     DKRREKANESKVMHVIGDVKGRTAILIDDMIDTGGTIVQAAQALMDNGAKVVYACCTH
                     PVLSGEAIQRINNSPLKELIATNTIPLSPAGKRSKKIKVLDVSPILGEAIKRIHSDAS
                     VSSLFI"
BASE COUNT         1032 a         1559 c         1373 g         1118 t
ORIGIN      
        1 cgtgaggctc cgcgccttcg gcatgttcaa tgtcagggtc atccaaccgt cgctgttcat
       61 caacaccctc gtcggaacga aggcgtccta caccgtcgag gacattgagg actacctggg
      121 caaggtcata ctctcccgct tcaacgacta tctcggcagc aacctcgaca ccctcctcga
      181 cctgcccggt cagtacgaca ccatgtcggc gggactgaca cagatgctgg agaaggactt
      241 ttcccatttc ggcatccagc tctccggcct ctacatcaat tccatcaccc ctccccagga
      301 ggtccagaag gcgatcgacg acaaaagcag gctggccatg ttcgacgacc ttaacaagct
      361 ggtcaagatg aaggctgcca tggcgatgga aagcgccgct cagaaccccg gcgaagcggg
      421 cgcgggcatg ggcatgggac tcggcttcat gatgcccgcc atgtacgcgg agaccctcaa
      481 gggagcaccg cgatccgacg gtggtatgaa atgccccgag tgccagaacc ctatcaacga
      541 ggatgcccgg ttctgcccgt cctgcggaca ccaccttctc gtgatctccc agtgcccgaa
      601 gtgccacaag aacctccccg cacgggcaag gttctgtcct gcctgcggcc agagtgtgac
      661 ggcaagggaa gagcttctga agtgccctca ttgcgggact gacaacctgt cgggatcggt
      721 cttctgcaac cactgcgggg aaaagatcta gccatcatgc agatcgaaca tcagtgcccc
      781 caatgcggcg ccccggtggt cctcgaggag accgacaggc tgcttacctg cgatttctgc
      841 aagaccaggc tctacatgca gccccgggac tactttcgat actgctttac ccccctggac
      901 ccttttctcg aacaggtgat ctacgtgccc tactggcgtt tcaggggtgt gcatttttcc
      961 tgcaggacaa gcgggataac caacgggatc ctggacaaga ccttccttgc cgtcgaaagc
     1021 aagcacttcg cgcaaaccct tggattacgg ccgcaagcgc tgaaactgcg ctttgtgcag
     1081 cccgcggaca acgccagatt catacctccc aatatccctt tcgagaagtc ttttgtggag
     1141 gcaaaacaca acgtgactta cgagacggtg acagttcacg aaacccgttt cgtccggcgc
     1201 gggacagagg gggactacgc ggagatcctc gtaacgagaa aggagctgaa agaggaaagg
     1261 ctctaccacg aagccttcgt cgcggacacg atgagcctta tctacacgcc gatgttcgcc
     1321 cgcaacggcc gttttcatga tgcagtactc aacgaagcgc tctacgaggg ccctcccgtg
     1381 tacgagtcat ttccgtacga gtttttctcc ggcggctggg cgataaactt cctgcccacc
     1441 ctctgccccg agtgcggctg gaacacactg gctgaaaggg acagctgcgt cctcttgtgc
     1501 aacgggtgca attccgcctg gaggatggat ggtcaaggtc ttggacgcgt cggtttcgcg
     1561 accgccctgt cgacgaaagc ggctccgggg tcggtgcgtc aatacctgcc cttctggcgg
     1621 ctccgggtcg aggtcgaggg tttgaaactc gactgttacg cggacctggt acgccttgga
     1681 aaccttccca gggtcataca accgggctgg gaagaacctc cctttttctt ctggacaccc
     1741 gcgttcagga tgacgccggc ggtctatcgg cgtatgtcaa agcagttcac cattgccaac
     1801 ttctccggtg aattccatga caggctgccc gacgcgcccg tgcaggccgt taacctcacc
     1861 cctcaggacg ccctcgacag cctgataacg atcatagccg acttcgccat caacaagaag
     1921 aaggtcctcc cccttcttcc cgacatgagg ataaccctcc gcgaggcact gctcgtactc
     1981 ttccctgtga ccgagaccga ccacgaatac atccaaccag acataaactg cggtctcatg
     2041 aagaacgtgc tggaacaggg gaaaaacatc taaagaccgt tgcagccgct tgaacccagg
     2101 tcaaacgttg aagcgtatgt ggagtatgtc cccatcctcc acaaggtagt cgcggccttc
     2161 gaggcggagg aggccttttt cgcgggcggc gtgttcgctg ccgcaggtga tgagatcgtc
     2221 gatgttgatg acctcggcgc gtatgaatcc cttctccatg tccgtgtgga tcttgcccgc
     2281 ggccttcgcg caggtagtcc cccggggtat cgtccaggcg cgcatctcct tgcccacgac
     2341 agtgtaaaag gtgatgaggc cgaggatgcg atagccgaca tggatgatct tttcgatgga
     2401 gaattccttc atgtcataga gttccatgaa ggaggcccgc tcgtcctccg gcagggcgct
     2461 caagtcctgt tccagcttgc cgcagatgta gacgacctgc cttcccctct tggcggcagc
     2521 ttcctcgatg gcgggttgcg gggacgccga cagaccttcc tcatcgacgt tggcgatgta
     2581 gaagaggggt tttgtggtga tgagatcgaa ggcacgcagg gcctgcctct cgttctcgtc
     2641 aaagtcgtcg gtggcaacga agcggccggc ctccaggtag cgcctcaccc tgaggagaag
     2701 ttccatctcc gcttcgccct tgtccttcga gatcttcgcg agccgttcta tcttccgtat
     2761 gcggtcctcg acgatctcga ggtcggagat gacgatctcc gtctccacga tatccatgtc
     2821 ccgggcgggg tcgatatcgg cgtacacgtg gggcacctgt ccggagttga aggcccggat
     2881 aacatgggca acggcatcga cgttcctgat atgggaaagg aacctgttcc ccagaccctc
     2941 ccccttgtgg gcgtccttga tgaggcccgc gatatcgaca aactcgatgg ttgtctgagt
     3001 gatcttctcg ggcttcaaga gacgggccag gctgaaaagt ctcgcgtcgg gaacggggac
     3061 gacacccacg ttgggatcga tggtgcagaa gggatatggc tggcagggaa ccgcgagtgc
     3121 ggtaagtgcg ttgaatatgg tcgatttccc cgaattggga agacctatga aaccgcacga
     3181 aaaactcatg tccttcgact cactccggtc cgcgccggtt aaagaggctc atcgccttgg
     3241 acccgccttc gaagacgaag acctttaagg catcgcaggc gatgtcgatg atatccgcga
     3301 gctccccggc ctcatccccg tcgaaccgtg acaacacgta ttcctcggca ggcatcgagg
     3361 gattcctccc tatgcctatc ttgagccgcc agaagtcctt gccccccagg cactctatca
     3421 ccgaccgcac tcccttgtga ccaccgtctc tgcctccgag acggatccgc atcttgccga
     3481 aatccatgtc catgtcgtcg tggataacga tcacgcggtc gggcggcacc ttcagttttc
     3541 ctgccagtct ggcgatgggg ccgcccgaga ggttcatgta cgtgtcaggc ttcgcgagga
     3601 ccagatcatc cttcgacccg atcctgcaac ctgaatcttt cttccgaacc ggtatcttga
     3661 aacgttccga aaaacggtcg atgacgagat acccgacgtt atgccgcgtg aaggcgtact
     3721 cggagccttt gttgccaagt ccgcacaata aatgcacgga cctttactcc tctttctcct
     3781 tcgcttcgga agcctcggcc gcggcttcct ctgtcgccac cctcttctct tccactgtag
     3841 gaggcacgac actgagaatg gccacatctc catgctcgat gagttttacg ccgggaaggg
     3901 atatatcgct cacgtggacg gaatcgccga tatcgagctc cgtcatgtct atgacgatct
     3961 cttccggtat ctggccggga aggcactcca ccttgatgga cctcagatgg acatcgataa
     4021 cgccgccgag gacctcgccc ttggacttgc ccttcaggtg gatggagact tccacctcga
     4081 ccgtcttctc catggagacc tggagaaaat cgatgtgcat gatctcagta cccagaccgt
     4141 tcctctgcac ctctttgacc ataacgggcc tgctctcgac gctatcggca ccatggatct
     4201 ccatgtcgag aatgacgttc ctcttcatgt gcctcgtgat cttgtcccag tccttcgatg
     4261 agatcgttat tgaaacggga tcaacaccgc ccccgtagag caccgccgga atcctccctg
     4321 ccgcccttgc ctttcgtgca acaccttttc ctgttgcgct tcttctgtct gccttgatca
     4381 aaacctgttc catgatgttc ctcccaacta tatgaacaac gaacttacgg acgcgtcgct
     4441 gtgtattctc tttatggcct ctccgagaat aggcgataca tccaacactt ttatcttctt
     4501 cgatctcttc ccagccggag aaaggggaat ggtattcgtc gctatcagct ccttgagggg
     4561 tgagttattg atcctctgga tcgcctctcc cgagagcacg ggatgcgtgc agcatgcata
     4621 gacgaccttt gctccgttat ccatgagggc ctgggccgcc tggacgatcg ttccgcctgt
     4681 gtcgatcatg tcatcgatga ggatggcagt cctgcccttc acgtcgccga taacatgcat
     4741 aaccttggat tcgttggcct tttccctccg cttgtcgatg atagcgatgg tggcgttgat
     4801 cctcttgccc agttcgcggg cccgttccac tccgccggca tcgggagaaa ccaccacgat
     4861 gtcccccttt atcttcttta tgtactcgaa ctgaacgggg agtgcgtaaa gatggtcgac
     4921 gggaatgtca aagaacccct gtatctggcc cgcgtggagg tccatggcca gtatcctcga
     4981 agctccggcc acggttatga ggttcgccac gagcctcgcc gagatggacg tacgaggaag
     5041 cacctttcgg tcctgcctgg catacccgta gtagggtatc ac
//
LOCUS       DAOQTR010000087         5077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_18198_length_5077_cov_3.342891, whole genome shotgun sequence.
ACCESSION   DAOQTR010000087 DAOQTR010000000
VERSION     DAOQTR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18198_length_5077_cov_3.342891"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKN85_03150"
     CDS             complement(<1..755)
                     /locus_tag="PKN85_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT43429.1"
                     /translation="MDYKDTLNLPKTPFPMRGNLPVKEKDMLARWQEIDLYRKMTEAR
                     KDSPAFILHDGPPYANGNIHLGTALNKILKDIIIKAKFMTGHRADYIPGWDCHGLPIE
                     HQVEKNVKQKKLELSKLETRKECRAYARQFIDIQREEFKRLGGIGDWDHPYITMDFDY
                     QATIIEEAAKFFIRGEVYRKKKPVYWCLSCETALAEAEIEYDTKRSSSIYVKFPYTGE
                     REPVFAGYPDKPVHMLIWTTTPWTLPANLAIAV"
     gene            complement(1033..1650)
                     /locus_tag="PKN85_03155"
     CDS             complement(1033..1650)
                     /locus_tag="PKN85_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNT43430.1"
                     /translation="MSIERVTIESDQSIEGLLRRQSRKAGVVICHPHPLYGGNMYNNV
                     VSAIEDGYAAQGFTTLIFNFRGVGDSKGEYDEGDGEVRDATAAYRVLKEQLDDDAHIT
                     LAGYSFGAWVISRSALGIDNFDSLFLVSFPCLIYKYDHLKTFSKEILIVGGTYDDIAP
                     MDDLYELYKSLTTLDKHLKVINTTHFYAGKETELIDFIKETIPKR"
     gene            1701..2564
                     /gene="accD"
                     /locus_tag="PKN85_03160"
     CDS             1701..2564
                     /gene="accD"
                     /locus_tag="PKN85_03160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNT43431.1"
                     /translation="MGLFKKKDKEKKPYREINIQKEISRAEKEESLWRKCNSCQELLY
                     RKEVERNRHVCPKCFYHFPISVENRIALTFDRGSFTEFHEKVEPVDFLKFKDTKPYKT
                     RLVETQETARRPDALMCGSAKIGGIPVLTAIFDFNFMGGSLGSVVGEKITRTLEEGAR
                     QKTPVILFCSSGGARMQEGIMSLMQMSKVSGAIYHLKSNGVPYITVLTDPTLGGVSAS
                     MGMLGDVIIAEPKAMIGFAGERVIKETIKAKLPPGFQRAEYLLEHGMIDMISSRKELK
                     QTLHTLLSLIA"
     gene            2561..3832
                     /locus_tag="PKN85_03165"
     CDS             2561..3832
                     /locus_tag="PKN85_03165"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNT43432.1"
                     /translation="MNDARYESSLEYLSGLERFGSVFGLENISRLLGAIGDPHRSLRT
                     VHVAGTNGKGSVATMISCILKAAGYRVGKYTSPHLVSFTERITVNEEEIGEDEVASLT
                     EHIRERAHGTGPDPFYTYFDFTTALAFEHFRREKIDIAVVETGLGGRLDSTNVIEPLT
                     TVVTNVAFDHMKELGGTIGKIAGEKAGIIKKGVPLITGARGRALEVLEARARELASPI
                     HVLGRDFLGRKKGERRFSYRGIGWDCDNIALNLEGDHQLFNAALALCAVESLSRGGFR
                     VGEDHVRDALASVRWQGRLETVRERPLVILDGAHNLSGVRALTRFLRSRYRDRRKVLV
                     FGVMRDKQYGRMLEVMNGCVDLAILTQPNTERALEAESMRGLARNPIVTKDTKSALRK
                     ARQLAGDRDLILVTGSLYTIGEAKRVVNEIF"
     gene            3819..>5077
                     /gene="lptD"
                     /locus_tag="PKN85_03170"
     CDS             3819..>5077
                     /gene="lptD"
                     /locus_tag="PKN85_03170"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNT43433.1"
                     /translation="MRYSRILVMVMMFAFLLPLVSRGQQIGVRGGDVKGPVQIIADQV
                     EHDRAANTYTARGNVEVREATRTLFADYIFLDDNTRDVTAEGNVIYEDLGDRIEAERM
                     QLNLETKKGTLEKARVYIKTGNVYVNGAEIEKTGESQYKIKKGKFTTCGWDKPEWTFT
                     SDEVDITVEGYAKTKSTTFHILGVPVFYIPWGIFPVKAQRQSGFLIPEMALSSADGAK
                     FKLSYFWAIAKDKDATMSAEYIEKRGVNLGTQFRYALREDLKGELEGNIINDRDYGNT
                     RYQVKAKHQQVFFNDLQFKANVWHVFDNDYLKDFGQSIQERSESQLKSTIFVEKPLPR
                     SLLTGGVTHFRNLLVKDNDTLFQYYPEVTYFTEYIPVFGGKMYLDLNSSLTNFYRDSG
                     GTYTRLAVDPSLRLPYSFKGLNTLLSGT"
BASE COUNT         1129 a         1366 c         1495 g         1087 t
ORIGIN      
        1 tggacggcga tggcaaggtt cgcgggaagc gtccagggtg tggtcgtcca gatcagcatg
       61 tggaccggtt tgtcgggata gcccgcgaag accggttctc tctcgcccgt gtaggggaac
      121 ttgacatata tggaactgga cctcttggtg tcgtattcga tctccgcttc ggcgagggcc
      181 gtctcgcagc tgaggcacca gtagacgggc ttcttcttcc ggtagacctc gccgcggatg
      241 aagaatttcg ccgcctcttc gatgatcgtc gcctggtagt cgaaatccat ggtgatatag
      301 ggatggtccc agtcgccgat gccgccgagg cgcttgaact cctcgcgctg gatgtctatg
      361 aactgccgcg cataggcccg gcattccttc cgggtttcca gcttggagag ctcgagcttc
      421 ttctgcttga cgttcttctc cacctggtgt tctatgggca ggccgtggca gtcccatccg
      481 gggatatagt ccgccctgtg gcccgtcatg aactttgcct tgatgatgat gtccttgagg
      541 atcttgttga gggcggtccc aaggtggatg ttgccgttgg cgtaaggggg gccgtcatgg
      601 agtatgaaag cggggctgtc ttttcgcgcc tccgtcattt tccggtagag gtctatctct
      661 tgccagcggg caagcatgtc cttttccttg acgggcaggt tgcctctcat gggaaagggc
      721 gtcttcggga ggttcaatgt gtctttgtaa tccatggcgg aacaatcctt gctgagatag
      781 aaagtaaccc ggccgggtcg gttaccttag gacgttttct tgtgatcggg aaatgttcta
      841 cgtttcaggt tgccgtaaac ccgctggatt ctaacaatca aattagcata gagtggggta
      901 tctattcaag aagaatgaag aaaaccacag cccctgaaac ccaaacaaag agattacgac
      961 cagcttgcgt cgtcccaggg gtcctacgcg tcctatacgt cccataggtc ctggagtcct
     1021 gcaggtccta ttctaccgct tcggaatggt ttcttttata aaatcgatca gttccgtttc
     1081 ttttccggca tagaaatggg tcgtattgat gaccttgaga tgcttgtcca gggtggtgag
     1141 gctcttgtag agctcataga gatcgtccat gggggctatg tcatcatacg tgccgccgac
     1201 gatgaggatc tccttgctga aagtcttcag atggtcgtac ttgtatataa gacacggaaa
     1261 tgaaacgaga aaaagactgt cgaagttgtc tatcccgagt gccgacctgc tgatgaccca
     1321 ggcaccgaag gagtagcccg caagcgtgat atgcgcatcg tcgtcaagct gttctttcag
     1381 gacccggtat gccgcggtgg cgtcacgaac ctctccgtca ccctcgtcgt attcgccttt
     1441 gctgtctccc acgccccgaa agttgaagat gagggtcgtg aagccctgcg cggcgtaccc
     1501 gtcctcaatg gcgctgacaa cgttattgta catgttcccg ccgtagaggg ggtggggatg
     1561 gcatatgacg acgcccgctt tcctgctctg ccggcggagc aatccctcga tactctggtc
     1621 cgattctatg gtgacccttt ctattgacat gaccatactt attttggtag tttttcaaca
     1681 aaaattcaac aggaaaagta atggggttat tcaaaaaaaa ggataaagag aagaaacctt
     1741 accgtgaaat aaatatccag aaagagatct ccagggcgga aaaggaagag tcgctctgga
     1801 ggaaatgcaa ttcctgccag gaactccttt acaggaaaga ggtcgagcgc aaccgtcatg
     1861 tctgtcccaa gtgtttctat catttcccca tctccgtgga gaacaggata gcgctgacct
     1921 tcgacagggg cagtttcacc gagttccatg aaaaggtcga gcccgtggac tttctgaagt
     1981 tcaaagacac gaaaccatac aagacgagac ttgtcgagac acaggaaacg gccaggcggc
     2041 ccgacgctct catgtgcggc agcgcgaaga tagggggcat tcccgttctc accgccattt
     2101 tcgacttcaa tttcatggga gggagcctcg ggagcgttgt gggcgagaag atcaccagga
     2161 ccctcgagga gggagcgcgg cagaagaccc ctgtcatcct cttttgctcg tccggcggcg
     2221 caaggatgca ggaaggcatc atgtcgctca tgcagatgtc gaaggtctca ggagccatct
     2281 atcacctgaa gagcaacggt gttccctaca taacggtgct caccgatccc accctcggcg
     2341 gtgtctctgc tagcatgggc atgctcggcg acgtcatcat cgccgaaccg aaggcgatga
     2401 tcggtttcgc cggggagagg gtgatcaagg aaacgatcaa ggccaagctg cctccggggt
     2461 ttcagcgggc ggaatatctt ctggagcatg gcatgatcga catgatctct tcccgtaaag
     2521 agctgaaaca gacgcttcac acgctgctgt ccctgatcgc atgaatgatg cccgctatga
     2581 aagctcgctg gagtaccttt caggcctcga gagattcggc tccgtttttg gactggagaa
     2641 catcagcagg cttctcggcg cgataggcga ccctcacagg tccttgagga cggtccacgt
     2701 cgctgggacc aacggcaagg ggtcggtcgc cacgatgatc tcctgcatat tgaaggcggc
     2761 gggataccgg gtgggaaagt acacgtcgcc ccacctggtc tcctttacag agcgcatcac
     2821 cgtcaacgag gaagagatag gggaggatga ggtcgcctct ctcacggagc acatccgtga
     2881 aagggcccat gggaccggac cggacccatt ttatacctac ttcgatttca cgacggcgct
     2941 ggccttcgag catttccgga gggaaaagat cgacatcgcg gtcgtggaga cgggcctcgg
     3001 cgggcgactg gattcgacga atgtcataga accgctgaca acggtcgtca cgaacgttgc
     3061 ttttgaccac atgaaggaac tgggagggac catcggcaag atcgccgggg aaaaggcggg
     3121 gatcataaag aaaggcgttc ccctgataac gggtgcccgg ggcagggccc tcgaggtgct
     3181 cgaggctagg gcccgggagc ttgccagtcc gattcacgtg ctgggaaggg atttccttgg
     3241 caggaagaag ggcgagaggc gtttctccta ccgcggtatc gggtgggatt gtgacaatat
     3301 cgccctcaac ctcgaggggg accatcagct tttcaatgcc gcccttgctt tgtgtgccgt
     3361 cgagtccctt tcccggggcg ggtttcgcgt cggagaggac catgtgagag acgctctggc
     3421 ctcagtgaga tggcaggggc gactggagac ggtgcgcgag cggccgctcg tcattctcga
     3481 cggtgcacac aacctttccg gggtgcgcgc tctgacaaga ttccttcgct cccgctaccg
     3541 ggaccgcagg aaggtcctgg tcttcggcgt catgcgggac aagcaatatg ggaggatgct
     3601 cgaggtcatg aacggctgcg tcgatcttgc catactcacg cagccgaaca cggagcgtgc
     3661 cctggaggcc gagagcatga ggggtctcgc ccgcaatccc atcgtcacga aagacacgaa
     3721 aagcgccctc aggaaggcgc ggcagctcgc cggggacagg gacctcatcc ttgtcacggg
     3781 ctcactttat acgatcggag aggccaaacg ggtcgtcaat gagatattct aggatccttg
     3841 tgatggtgat gatgttcgcc tttctcctgc ccctcgtatc ccggggacag cagatcggtg
     3901 tccgcggcgg ggacgtgaag ggtcccgtgc agatcattgc cgaccaggtg gagcatgacc
     3961 gcgcggccaa cacgtacacc gcccgcggga acgtcgaggt cagggaggcg acgcgcaccc
     4021 ttttcgcgga ttacatcttc ctcgatgaca acacacgtga cgttaccgct gaaggcaacg
     4081 tcatatatga agacctggga gaccggatcg aggccgagcg gatgcagctc aatctcgaga
     4141 ccaagaaggg gaccctggag aaggcgcggg tctacataaa gacgggcaac gtctatgtca
     4201 acggcgccga gatcgaaaag accggggagt cccagtacaa gatcaagaaa gggaaattca
     4261 ccacctgcgg ctgggacaag ccggaatgga cgttcacctc cgacgaggtg gacatcaccg
     4321 tcgaagggta cgcgaagacg aagtccacga cgttccacat cctcggcgtc cccgtcttct
     4381 atatcccctg ggggatcttt cccgtcaagg cgcagagaca gtcgggattt ctcataccgg
     4441 agatggcgct ctcttcggcg gatggggcca agttcaaact gtcctacttc tgggccatag
     4501 caaaggacaa ggacgccacg atgtccgccg aatacatcga aaagaggggc gtcaacctgg
     4561 gcacccagtt ccgctatgcc ctgagggaag accttaaggg agagctggaa ggcaacatca
     4621 tcaatgacag ggattacggg aacacgcgat atcaggtgaa ggcgaagcac cagcaggtct
     4681 tcttcaatga ccttcagttc aaggccaacg tatggcatgt cttcgacaac gactatctca
     4741 aggatttcgg gcagtccata caggaacgga gcgagagtca gctcaagtcg acgatcttcg
     4801 tggagaagcc cctgccccgg tccctcctta ccggcggggt cacacacttc aggaaccttc
     4861 tcgtgaagga caacgacacg ttgttccaat attatcccga ggtgacgtac ttcaccgaat
     4921 acatacctgt gttcggtgga aagatgtacc tcgacctcaa cagctctctg accaatttct
     4981 atcgcgactc gggagggacc tatacgcgcc ttgccgtcga cccgtcattg agactgccat
     5041 actccttcaa gggtctgaac acgcttctca gcggtac
//
LOCUS       DAOQTR010000088         5026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_18459_length_5026_cov_3.830618, whole genome shotgun sequence.
ACCESSION   DAOQTR010000088 DAOQTR010000000
VERSION     DAOQTR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18459_length_5026_cov_3.830618"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /gene="hydG"
                     /locus_tag="PKN85_03175"
     CDS             <1..1111
                     /gene="hydG"
                     /locus_tag="PKN85_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNT43434.1"
                     /translation="GYRRDNTIARRKLGLDEIEREVIALETMGHKRIALEVGEDPVNC
                     PLDYVIEAMGRIYSVKEKMGSIRRINVNIAATTVEEYRRLKAAGIGTYILFQETYHRE
                     TYGKMHPAGPKRDYDWHTMAMDRAMEAGIDDVGLGVLFGLYDFKFEVLGLLLHSLHLE
                     ERFGVGCHTISVPRMRPAEGVNVQTFPHLVSDDDFKQMVAVIRLAVPYTGMILSTREP
                     RGYRDEVIALGISQISAGSCTGVGGYHRDIERPAEDTGQFQVEDHRTTDEMLQSVCQA
                     GYLPSFCTACYRQGRTGDRFMALAKTGEIQNICQPNAILTFKEFLLDYASERTKKVGE
                     EALLRHLESVPNAKLREEVRRRLERLEAGERDLYF"
     gene            1224..1670
                     /locus_tag="PKN85_03180"
     CDS             1224..1670
                     /locus_tag="PKN85_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT43435.1"
                     /translation="MKTAKDMLKDKKKDIWSIGPNKTVFDALKIMGEKEVGALMVIDD
                     KGKVHGIISERDYARKIILKGKTSAQTKVSEIMTPADKMFTVKPETSVEDCMVLITAK
                     RIRHVPVFDNDKFVGLISIGDVVKSIISEKDMLIEHLSNYIAGKYV"
     gene            complement(1674..2711)
                     /gene="hydE"
                     /locus_tag="PKN85_03185"
     CDS             complement(1674..2711)
                     /gene="hydE"
                     /locus_tag="PKN85_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNT43436.1"
                     /translation="MADEYTRERALEMLGATDGALEELYRHADAVRKNHMGDDVYIRG
                     IIEFSNICVNDCLYCGIRASNRNVARYVMSPDEILAIARTMGRFRQTTVVLQSGETPG
                     IADEKLGVLIRRIKDATGLAVTVSVGNRPLETYRYWRECGMDRYLLRFETSDPALFAR
                     LHPGSSLDDRLRCIEDLKDLGVQTGSGFMIGLPGETVEVLADNILLCRRLDLDMIGIG
                     PFIPHPDTPLCGTRNAYHDDPDMFFRALAVLRIFNPDAHIPATTAFDAVFPGEGRNLA
                     LNRGANVFMPNVTPVRYKKEYLLYPGKPSVDEEPEECTTAAIARIESLGRRVGRGPGH
                     SFKKREQPGQR"
     gene            complement(2860..3189)
                     /locus_tag="PKN85_03190"
     CDS             complement(2860..3189)
                     /locus_tag="PKN85_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT43437.1"
                     /translation="MKKQNVFSHIPSDIPDEIFETIAARGTVRIERIVSRGQATPQGS
                     WYDEELAEWVILLKGSADILFEGEATAVTLLPGDHILIPPHRRHRVTRTDDEAPTIWV
                     AVYFADT"
     gene            complement(3254..3742)
                     /locus_tag="PKN85_03195"
     CDS             complement(3254..3742)
                     /locus_tag="PKN85_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT43438.1"
                     /translation="MEKDRLLGASALSLLTAGLLFCLCSCSLPRILVLNDPLTPQEHI
                     NLGVTYERSGELDEALKEYEKASRTLPVAHLYMGNVFFQKKEFAKAEEHYREAIDRTG
                     DPGAYNNLAWLYYSTDKNLDEAEKLARKAVELAPDKKEFKDTLQKIREKTMPNDQITI
                     TK"
     gene            complement(3697..4257)
                     /locus_tag="PKN85_03200"
     CDS             complement(3697..4257)
                     /locus_tag="PKN85_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNT43439.1"
                     /translation="MAELLPMKTLPFLCAMLLLFLGCTSGPALLPEGARVLSGVPFFP
                     QEDYRCGPAALATVMNYWYARSGSAVRTSIDEIAQAIYSPGARGVLPTDLENYPRKKG
                     FVTEQFTGSIGRLRENIDRGIPVILLVDHGLSVYRLDHFLVATGYTGDGIIVNSGREE
                     RQVVSNSELEKAWKKTGYWALRLFPS"
     gene            complement(4305..4694)
                     /locus_tag="PKN85_03205"
     CDS             complement(4305..4694)
                     /locus_tag="PKN85_03205"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HNT43440.1"
                     /translation="MARKTLVYYLIAVMFIIGIAPRVDAAFLPSETIGITGAQRDADI
                     QRIQAVLEMKLVRQRLLDLGFSPEEIATRVSEMTDEQVHSFAQKLDDLRVGKDSGLGI
                     VIAVLIIVLLVILIINLTTGKKVVVTQ"
     gene            4922..>5026
                     /locus_tag="PKN85_03210"
     CDS             4922..>5026
                     /locus_tag="PKN85_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43441.1"
                     /translation="MSTIYDVYAREILDSRGNPTVEVEVALTSGVIGRA"
BASE COUNT         1063 a         1383 c         1514 g         1066 t
ORIGIN      
        1 cggatacagg cgggacaaca ccatagcaag acggaagctc ggtcttgacg agatagagcg
       61 cgaggtcatt gccctcgaga cgatgggaca caagcgaata gccctcgagg tcggagagga
      121 tcccgtgaac tgtccccttg actacgtcat cgaggccatg gggcggatat attccgtcaa
      181 ggagaagatg ggcagtatac gccgcatcaa cgtcaatatt gccgccacca ccgtggagga
      241 gtaccgcagg ctcaaggcgg ccggtatagg aacctatatc cttttccagg agacctatca
      301 ccgcgaaaca tatggcaaga tgcatccggc ggggcccaag agggactacg actggcacac
      361 catggcaatg gaccgggcca tggaggcagg catcgacgac gtgggactgg gtgtcctctt
      421 cggcctgtat gatttcaagt tcgaggtcct tggcctgctc cttcattcgc ttcatcttga
      481 agagcgtttc ggggtcggct gccacaccat atccgtgccg aggatgcgac ctgcagaggg
      541 ggtcaatgtg cagacatttc cccaccttgt cagtgacgat gatttcaagc agatggtggc
      601 ggttatcaga cttgccgtcc cgtacacggg tatgatcctt tccacgaggg agccccgggg
      661 ctatcgcgac gaggtcatcg cgctcggcat ctcgcagatc agcgcgggtt cgtgcacggg
      721 ggtcggagga tatcaccggg acatagaaag gcctgcggag gacacgggcc agttccaggt
      781 ggaggaccat cggaccaccg acgagatgct ccaaagcgtt tgccaggcag gataccttcc
      841 gagcttctgc accgcctgct accggcaggg aaggacgggc gacaggttca tggccctcgc
      901 gaaaacgggg gagatacaga acatctgcca gcccaacgcg atcctgacat tcaaggaatt
      961 tctcctggac tacgcctcgg aaagaacgaa gaaagtgggt gaagaggcgc tcctcaggca
     1021 tctggaatcg gtgcccaacg cgaagctgcg cgaagaggtc agacggcggc ttgaaagact
     1081 cgaggcagga gaacgagatc tctatttttg agaagggtgc caaaatgccc ttgcctgccc
     1141 cgtgctttga ttatatactt tagcaacacg ggcttggatc tgcacagggg tcatgaaaaa
     1201 aaagagaagg gggcttaaga gctatgaaga ccgcaaagga catgctcaaa gacaagaaga
     1261 aggacatttg gtccatcgga ccgaacaaga ccgtttttga cgcgctgaag atcatgggtg
     1321 agaaggaggt tggcgcgctg atggtcattg acgacaaagg aaaggtgcac gggataatat
     1381 cggaaaggga ctacgcgcgc aagatcatcc tcaaggggaa gacgtcggcc cagaccaagg
     1441 tgtcggagat catgaccccg gcggacaaaa tgttcacggt gaagccggag acatccgtcg
     1501 aggactgcat ggtcctcatc accgcgaaac gcataaggca cgtccccgtg tttgacaacg
     1561 ataagttcgt cggccttatc tccatcggtg acgtggtcaa atcgatcata tccgagaagg
     1621 acatgctcat cgagcatttg agcaattata tcgccggcaa atacgtttaa agatcacctc
     1681 tgtcccggtt gctccctttt cttgaaggag tggccgggac ctcgtcccac tcttctcccc
     1741 agggactcaa ttcgtgctat cgccgcggtc gtgcattcct cgggttcttc gtccaccgaa
     1801 ggctttcccg gatagagaag gtattccttt ttgtagcgga cgggagttac attgggcatg
     1861 aagacgttcg cgcccctgtt gagggccagg ttccggccct cgccggggaa gacggcgtcg
     1921 aaggccgttg tggccggtat gtgggcatcg gggttaaaaa tgcgcagcac cgcgagggcc
     1981 ctgaagaaca tgtcggggtc gtcgtgatag gcgttcctcg tgccacacag aggggtgtcg
     2041 gggtggggga tgaaggggcc gatcccgatc atgtcgaggt caaggcggcg gcacaggagg
     2101 atgttgtccg ccagcacttc cacggtctca cccgggagcc ctatcatgaa cccgcttccc
     2161 gtctggaccc cgaggtcctt gaggtcctcg atgcagcgca gcctgtcgtc gaggctcgat
     2221 cccggatgga ggcgcgcgaa aagtgccggg tccgatgtct cgaaacggag aagatatcgg
     2281 tccatgccgc actcgcgcca gtatcggtat gtctcgaggg gcctgttgcc cacggaaacg
     2341 gtaacggcca gacctgtcgc atccttgatc cgtcttataa ggacccccag cttctcatcg
     2401 gcgatccccg gcgtctcgcc cgactggagg acaacggtgg tctgacggaa acgtcccatg
     2461 gtacgggcta tcgccagtat ctcgtcgggg gacatcacgt accgggcgac gttcctgttg
     2521 gaggcccgta tcccgcagta gaggcaatca ttgacgcata tgtttgagaa ctcgatgatc
     2581 ccccgtatgt agacgtcatc tcccatgtgg ttcttccgca ccgcgtcggc gtgacggtag
     2641 agttcctcga gcgctccgtc ggtcgcgcca agcatctcga gggcgcgttc ccgtgtgtat
     2701 tcatctgcca tggctgacaa tatacccgat aagagagcgt ctttgcaacc ggggaggcgt
     2761 catatcgacg acgcgtgagc cggcaacagg gcttctcccc gtccgccccg tcgcgcgtga
     2821 agcgcgcgat ccttgtgccc gttcatgttt gacacatctt cacgtgtcag caaaatacac
     2881 cgcaacccat atcgtgggcg cctcgtcgtc ggttcgtgtc actctgtggc gccgatgcgg
     2941 cgggatgagg atgtggtccc ccggaagaag ggtgacggcg gttgcctcgc cctcgaagag
     3001 gatgtcggcg cttcccttga ggagaatgac ccattccgca agctcctcgt cgtaccagga
     3061 tccttgcggc gtcgcctggc cgcgggagac gatcctttcg atcctgacgg tcccccttgc
     3121 cgcgatggtc tcgaatatct cgtcgggaat gtcggacggg atgtgggaga agacgttctg
     3181 ctttttcatg gtatcctcga tggaagaaga caattaccaa tgaagctaat aatgaccaat
     3241 gatcaaattc caattatttg gttattgtga tttggtcatt gggcattgtc ttttccctta
     3301 ttttctgcaa agtgtctttg aattcctttt tgtccggtgc cagctcgacg gcctttcgtg
     3361 cgagcttttc cgcctcgtcg aggttcttgt ccgttgaata atagagccag gcgaggttgt
     3421 tgtatgctcc ggggtcgccg gtcctgtcga tcgcctcgcg gtagtgttcc tcggccttcg
     3481 cgaactcttt cttttggaag aagacgttgc ccatgtagag atgggcgacg ggcagggtcc
     3541 tcgaggcttt ttcgtattcc ttcagcgctt cgtcgagttc tcccgacctt tcataggtca
     3601 ccccgaggtt tatatgctcc tgtggtgtca aggggtcatt gaggacgagt atcctcggaa
     3661 ggctgcagga gcagaggcaa aagaggagac ctgccgtcag gagggaaaga gccgaagcgc
     3721 ccagtaacct gtctttttcc atgctttctc cagttcggaa ttggaaacga cctgtctctc
     3781 ctcgcggccc gaattcacga tgatgccgtc cccggtatag cccgtcgcca ccaggaagtg
     3841 atcgagccga tagacggaca ggccgtggtc gacaagaagg atgacaggga tgcccctgtc
     3901 gatgttctcg cgcaggcggc ctatggaacc tgtgaactgc tccgtcacga agcccttctt
     3961 tctcgggtag ttctcgaggt ccgtgggcag gacgccccgc gccccggggc tgtagatggc
     4021 ctgagctatt tcatcgatac tggtcctgac ggcgctgccg ctgcgggcat accagtagtt
     4081 catgaccgtc gcgagggcgg ccggaccgca ccgatagtcc tcctggggga agaaggggac
     4141 accggaaagg accctcgccc cttcaggcag cagggcgggt ccggaggtgc agcccaggaa
     4201 gagaaggagc atggcgcaca ggaacggcag ggtcttcatg gggaggagtt cggccacggg
     4261 gcggccgaaa ggcaccccgt ggcgcttttg tcgcgaatgc tccgctattg cgtcaccacg
     4321 accttcttgc ccgtggtgag gttgatgatt agaatgacga gcagtacgat aatgagaacg
     4381 gctatgacga taccgagccc gctatccttg cccactctca gatcgtcaag cttctgggcg
     4441 aaactgtgga cctgctcatc cgtcatttcg gagacccgtg tcgcgatctc ttccggagag
     4501 aaaccgagat cgagaagcct ctgcctcacg agtttcattt cgaggaccgc ctgtatcctc
     4561 tggatatcgg cgtctctttg tgccccggtg atgccgatcg tctccgacgg gaggaacgcg
     4621 gcatcgacac ggggggcaat gccgatgatg aacatcactg cgatgagata ataaaccaaa
     4681 gtctttctgg ccatgccgtc acctcctttc agatcgaccc gactgatcgt atgtaagagt
     4741 atatatccga tataccgcgg tttgtaaacc cttttgaggc ctgccgcgaa ggcccgcatt
     4801 ccggtgagcc gcgttcgtaa caacatatga ccattgaagg acatcgactc ctgtggtatc
     4861 ctgcattcat atcggccggt ggtttgtgag gccggtcccc ttacaaaaca aggaggttct
     4921 catgtcaacg atatatgatg tatacgcgcg ggaaatactg gacagcaggg gaaatcccac
     4981 cgtcgaggtc gaagtcgcgc tcacctccgg cgtcatcggc cgcgcc
//
LOCUS       DAOQTR010000089         4950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_18858_length_4950_cov_3.960368, whole genome shotgun sequence.
ACCESSION   DAOQTR010000089 DAOQTR010000000
VERSION     DAOQTR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18858_length_4950_cov_3.960368"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PKN85_03215"
     CDS             <1..1028
                     /locus_tag="PKN85_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43442.1"
                     /translation="LLERSISSVAGRQEVSAVLTDSGEEIPCDMLVLSMGVRPDTRLA
                     RGCGLAIGHLGGIKVNPLMMTSDPDIFAAGDAVEVNDLVTGFPVLTALAGPANKQGRV
                     AADNALGRRTVYRGTLGTSIVKVFGLTVSSTGASEKTLRMREVPHLVSYTHSNSHAGF
                     YPGAEMMTIKLAFSPGSGRILGCQIVGGAGVDKRIDVMATAIRGSMTVFDLEDIDLAY
                     APPFSSARDPVNIAGFVASNILKGDHEVVHAGDLKDIGENAVLLDLRDMEDLVRSSPI
                     EGAVHVPLGRLRKKMEKLDREKLYITYCEVGTRSYVGHRVLAQRGFKSKNLSGGYKTY
                     AATMKGN"
     gene            1210..1644
                     /locus_tag="PKN85_03220"
     CDS             1210..1644
                     /locus_tag="PKN85_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNT43443.1"
                     /translation="MTLFPALPGLSAPDLPNVSVVFFDAKDRELCRFEAEPAVTPEQQ
                     AQGLMFRSSLRRRTGMLFLHDRDEVQNYWMKNTWIPLDLIFINGRYEVVHVHRGARPR
                     DETNISSRYPARYILEVNAGEARECAITRGVRVRFGGVPAGR"
     gene            2184..2375
                     /gene="rpmB"
                     /locus_tag="PKN85_03225"
     CDS             2184..2375
                     /gene="rpmB"
                     /locus_tag="PKN85_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNT43444.1"
                     /translation="MARVCEICGKGKQIGYNVSHANNKTKREWRPNLQTVRIVKNGET
                     LKARLCTKCIKKGDFQKAV"
     gene            complement(2565..4703)
                     /locus_tag="PKN85_03230"
     CDS             complement(2565..4703)
                     /locus_tag="PKN85_03230"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNT43445.1"
                     /translation="MIRFNDIVDEVLKYNPDADVELLQKAYIFSAKAHKGQVRLSGEP
                     YLIHPLETAYTLTKMNLDIPSIVSGLLHDTIEDSYVDKKEIEEYFGKEIAELVDGVTK
                     ISKIPLKTSEESRVENFRKMILAMSKDIRVILVKLADRYHNMQTLNFLSAEKQIEIAR
                     ETFEIYAPLAHRLGIEWLRAELEDVAFKYLKPTEYRLIADNIAQKKREREAYINEVIG
                     LLRQKLDEFHVNADIFGRAKHLYSIYRKMSLEGLNIDDIYDIIAFRVVVDTIKECYEA
                     LGIIHAAYKPIPGKFSDYIALPKANMYQSLHTKIMGPHGEKVEIQIRTREMHRLAEEG
                     IAAHWKYKEGKVFNPKEDKIFAWLRRIIEWQQELKDNKEFMEIFKIDLFPDEVYVFTP
                     NGDVRELPKGATPVDFAYAIHSELGHRCVGAKVNGKLVPLRYTLKSGDTVEIMTSPTH
                     KPSKDWLSFVSTSKAKTKIRQWIKTEQREKSIELGRSLLDKELAKHSMSFSRLLKSGD
                     LLPLAKDFSFESVDDLFASVGYGLYTPLQVLNKIIPEEEKPAGKIKTIIHSLKKGRDN
                     AIKIKGIDGLVIRFARCCNPIPGDNIIGFITRGRGLTVHVEDCPNVHTYDEQRKVEVS
                     WQLSKDYTFPVRFKVTGNDRKGLLSDISSVIAANKVNILSASAMTYPDRSAAGIYEVE
                     IEHMSQLQKVMKSIQKIKGVRSVDRMRSIT"
BASE COUNT          958 a         1384 c         1424 g         1184 t
ORIGIN      
        1 tactgctcga acggagcatc tcctccgtgg cggggcgaca ggaggtctca gctgtgctga
       61 cggacagcgg cgaggagatc ccctgcgaca tgctcgttct ttcgatggga gtccggccgg
      121 acacaaggct tgcccggggc tgcggtctcg cgatcggcca tcttggaggc ataaaagtga
      181 acccgttgat gatgacctcc gatccggata ttttcgccgc cggggacgcc gttgaggtca
      241 acgatctcgt caccggcttc cctgtgctga ccgcactcgc cggcccggcc aacaagcagg
      301 gcagggtcgc ggcggacaat gcactgggaa gacgaacggt ctacaggggc acactgggga
      361 cttcgatcgt gaaggtcttc ggcctcacgg tgtcgtccac gggtgccagc gagaagacgc
      421 tgcgcatgcg cgaggtgccg catctcgtca gttacacgca ttcgaactcg cacgcgggat
      481 tctatcccgg ggccgaaatg atgaccatca aacttgcctt ctcaccggga agcggccgga
      541 tccttggatg ccagatagtg gggggagccg gggtggacaa gcgcatcgat gtgatggcaa
      601 cggccattcg cggttccatg acggtctttg acctcgaaga tatcgacctg gcgtacgcgc
      661 ctcctttctc gtcggcgaga gaccccgtca acatcgccgg tttcgttgcc tccaacatat
      721 tgaaggggga tcacgaggtc gtccacgccg gggatctgaa ggatatcggg gagaacgcgg
      781 tccttctgga cttgagggac atggaggatc tggtgaggag ttctccgatc gaaggggccg
      841 tgcacgttcc cctcggacgg ctgagaaaga agatggagaa actggacagg gagaagcttt
      901 acatcaccta ctgcgaggtt ggaacgcggt cctacgtggg ccatcgcgtt ctggcgcaga
      961 gaggtttcaa gtcgaagaac ctcagcggcg gatacaaaac gtacgccgcc accatgaaag
     1021 gaaattgatc ctctgcccgg ccgtcatagg ccggagaggg cggtccatcg cctgatcgcc
     1081 acggtgggat tgtcttcttg acttttccac ctgtccggcg caccataagg aatgaaagat
     1141 cccatgactg ccgcgtcgcg cacagcgttc ttccgccggc cctcctttcg tctctgtttc
     1201 ctgatggtct tgacgctctt tcctgcttta cccggtcttt cagctccgga ccttcccaac
     1261 gtcagcgtcg tgttcttcga tgctaaagac cgtgaactct gccgtttcga agcggagccg
     1321 gccgtgaccc ccgaacagca ggcgcagggg ctcatgttca ggtcctctct gcgcaggcgc
     1381 acgggaatgc ttttcctcca tgaccgcgac gaggtccaga actattggat gaaaaacacc
     1441 tggatccctc tcgacctcat cttcatcaac ggacggtacg aggtcgtcca tgttcaccgg
     1501 ggcgcccgcc cgcgcgatga aacgaacata agctcacgat atcccgcccg gtatatcctg
     1561 gaagtgaacg cgggcgaggc gagggagtgt gccatcacgc ggggtgtccg ggtccgtttc
     1621 ggaggggtcc ccgccggtcg ctgacaggtt tcctccggac cgggtgaagc cactgctttt
     1681 ccacaacgat tgtggatttc ctgttgataa gttgtggaaa tgcgcggtca acctcgtgat
     1741 gctaaaaggt tttgctcctt ttgcccggtt accgtgcatg gtgaaaacag caatcatttc
     1801 aacgcccata gagataattc accgatttga tgccttttcg taatggtccg acgctggaat
     1861 gcttttagcc aatcatttac aggggttcga ccggtatctc ccggcaccta tccgtatctt
     1921 atcgaaatca ttcactaaaa atagcgagaa actccaaggt gaggaatgaa ggacaaccgc
     1981 atctgatgcg tcccatatgt cctgtatgtc gtatcggtcc tgtccattcc catgcgtcct
     2041 gtgaagggcc tgcccctatc cccttttctg ttccccgatc ggccccctgc cgacaaaagt
     2101 gttgacatag aacgccttaa gcgtgtaaaa tctttgtctt taaaggaact caaggaagtt
     2161 cggacaaggt caggaggtca caaatggcaa gggtctgtga gatctgtgga aaaggaaaac
     2221 aaatcggtta caacgtgagc cacgcgaaca acaagaccaa acgggaatgg cggccgaacc
     2281 tgcagaccgt ccgcatcgtg aagaacggcg aaaccctcaa ggcaaggctc tgcacgaagt
     2341 gcatcaagaa aggtgatttt cagaaagcag tgtagtgaag acaggtgatg ggtcatgggt
     2401 tacaggtgat aggaaagtcc atcaccttta cccccctacc gatatacgac gttcataccc
     2461 ccatcgagcg attctcaaac gaatacaggt gaccggtaac cggcgtcttt gtcttcccat
     2521 cacccataac ctattaccca ttacccatct cctgtctccc gtctctaggt tatactcctc
     2581 atcctgtcga cggagcgcac tccctttatc ttttgaatgg acttcatgac cttctgcagc
     2641 tgtgacatgt gctcgatctc gacctcgtag atccccgccg ccgatctgtc gggataggtc
     2701 atcgccgagg cactgaggat gttgaccttg ttggcggcta tgacgctcga gatgtcggag
     2761 aggagcccct tgcggtcatt tcccgtcacc ttgaacctca cggggaaggt gtagtccttg
     2821 ctgagttgcc agctcacctc caccttgcgc tgctcgtcgt aggtgtggac gttcgggcaa
     2881 tcctcgacgt ggacggtgag gccgcgaccc cgggttatga aaccgatgat gttgtcgccg
     2941 gggatgggat tgcagcatcg cgcgaaacgt atcacgagcc cgtctatccc cttgatcttt
     3001 atcgcgttgt cccgcccctt cttcagtgag tggatgatgg tcttgatctt ccccgccggt
     3061 ttttcctcct cggggatgat cttgttgagt acctgcagcg gagtgtacag cccatagccc
     3121 acgctggcaa agagatcgtc gacgctctcg aagctgaaat ctttggcaag gggcaggaga
     3181 tcgccgctct tgaggagccg ggagaagctc atcgaatgct tggccagttc cttgtcgaga
     3241 aggctgcggc caagctcgat gctcttttcc cgctgttccg tctttatcca ctgcctgatc
     3301 tttgtcttcg ccttggaggt cgatacgaag ctcagccagt ccttgctcgg cttgtgggtg
     3361 gggctcgtca tgatctccac ggtgtcgccg ctcttcaagg tgtagcgaag gggcaccagc
     3421 tttccgttca ccttggcgcc gacgcaccgg tgacccaact ccgagtggat cgcgtaggcg
     3481 aaatcgacgg gagtcgctcc cttggggagc tctctcacgt cgccgttcgg cgtgaagacg
     3541 tatacctcgt cggggaagag gtcgatcttg aagatctcca tgaactcctt gttgtccttg
     3601 agttcctgct gccactcgat gatccggcgc agccacgcga agatcttgtc ctccttggga
     3661 ttgaagacct tgccttcctt gtatttccag tgggccgcga tcccttcctc cgccaggcgg
     3721 tgcatctccc tggtccttat ctgtatctcg accttctcgc cgtggggacc catgatcttg
     3781 gtgtgcagag actggtacat gttggccttc gggagggcga tatagtcgct gaactttccc
     3841 ggtatcggct tgtaggcggc gtggatgatg ccgagcgcct cgtagcactc cttgatcgtg
     3901 tcgacgacca cccggaaggc aatgatgtcg tagatgtcgt cgatgttgag cccctccagt
     3961 gacatcttgc ggtagatact gtagagatgc ttcgcccgcc cgaatatgtc ggcgtttaca
     4021 tggaactcat caagtttctg gcgcaaaagc ccgatgacct cattgatgta ggcctcgcgt
     4081 tccctcttct tctgggctat gttgtcggcg atgagcctgt attcggtcgg tttgaggtac
     4141 ttgaaggcca cgtcctccaa ctccgccctg agccactcta tacccagcct gtgtgccagg
     4201 ggcgcgtata tctcgaaggt ttccctggcg atctcgatct gtttttccgc tgacaggaag
     4261 ttcagggtct gcatgttatg gtagcggtcg gcgagcttga cgaggatcac ccgtatgtcc
     4321 ttgctcatgg caaggatcat tttgcggaag ttctcgacgc gggactcctc cgacgtcttt
     4381 agtgggatct tgctgatctt cgtcacaccg tcgacgagtt cggctatctc cttgccgaag
     4441 tattcctcga tctctttctt gtcgacgtaa gagtcttcaa tggtgtcgtg gagaagaccc
     4501 gagactatgc tcgggatgtc gaggttcatc ttcgtcaggg tgtacgccgt ttcgagaggg
     4561 tgtatgaggt acggttcccc ggagaggcgg acctgtccct tgtgcgcctt cgcggagaag
     4621 atgtatgcct tctgtagcag ttcgacatcg gcgtcggggt tgtacttcag gacctcgtcc
     4681 acgatgtcgt taaagcgtat catgcgtttt cccgtctgtg ttcgacaagc ggggcgtcgt
     4741 tgcgggaggt ctctctaaag aaggatggat cgggttctct tcgtctgctt tcctggccgt
     4801 gctcgggggg aatatctgat cgcatgtctt tcctgaccct tatattacct tatgcggcgc
     4861 gaaatgacaa cgggtaatga agcgcgcggc attgcgggac tcgggaaggt ccccgtttca
     4921 gggttcgagg gtggccgtgt ctgaccgggg
//
LOCUS       DAOQTR010000090         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_18900_length_4942_cov_3.764068, whole genome shotgun sequence.
ACCESSION   DAOQTR010000090 DAOQTR010000000
VERSION     DAOQTR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18900_length_4942_cov_3.764068"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(424..786)
                     /locus_tag="PKN85_03235"
     CDS             complement(424..786)
                     /locus_tag="PKN85_03235"
                     /inference="COORDINATES: protein motif:HMM:NF036460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HNT43446.1"
                     /translation="MKKISAVLATVLLLAAVRVCAGDPSETARIQYLLASVEALEGAT
                     FIRNGSEHDARAAAAHLRLKLKAAGNKVQTAEDFIKFCASRSSMTGESYLIRLADGTT
                     VQSEAFFRNKLRTFTGGQ"
     gene            complement(806..2038)
                     /locus_tag="PKN85_03240"
     CDS             complement(806..2038)
                     /locus_tag="PKN85_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT43447.1"
                     /translation="MKRNKRNVLCLLVLSFILASLLSLPACVTRPDIPAAGARQVSDH
                     FDGARYVNPGAPQALYSVSGQTTKRSATWWVWKWIFRVGWPEWPEQKEFLPGPPPVAR
                     APEGSIHVTPVGHATFLIQMDGLNILTDPIWSERCSPVSWVGPERHSEPGIRFGDLPP
                     VDVVLVSHNHYDHFDIPTLKRLAKKGTPRAVVPLGNYGLMRSTGMPAVDELDWWQSVP
                     LSSGVTVTLVPAQHFSGRTLWDRDKTLWGGFVISGPSGNVYYAGDTGYGPHFREIARR
                     FSPIRVALLPIAPFRPEAGEATAQHHHHSIVHMGPAEAVMAHRDLGEPLSIAAHFQVF
                     RLGVEGFTDAPDVLAASLKEHGLAPDAFVAPVFGQVIRIPPALSASLPPPDKTYYPIG
                     QIQGFIGSGPSPRHEALF"
     gene            2832..3746
                     /locus_tag="PKN85_03245"
     CDS             2832..3746
                     /locus_tag="PKN85_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNT43448.1"
                     /translation="MKININEYIQEFVDWFLTSGLRVGITLILTFVALKAAGFFAARL
                     IAALSRHRDDVEYQKRTRTLGSIVRYVLNTAVLIIAAMIVLGELGIEIGPVLAAAGIV
                     GLAVGFGAQSLVKDVISGFFILMEDQIREGDVVQIGGQGGLVEKISLRTTKLRDLAGN
                     VHYVPNGKIDVVTNMTKEYSRYVFDIGVAYREDVDEVIGLIREVDEELRGDPAFKDDI
                     LEPMEVLGLDQFGESALVIKARTKTLPIKQWGVAREFNRRLKMKFDENDVEIPFPHVT
                     LYMGQDKKGHAPPLHLAREGNPGRSEDV"
     gene            3816..>4942
                     /locus_tag="PKN85_03250"
     CDS             3816..>4942
                     /locus_tag="PKN85_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNT43449.1"
                     /translation="MRLIAGKKRAFWWVFLALVGVCLIVLSLTAGKLVEWLWMKQLGF
                     VGIFWRLLFSKLLLFVAAFLFVFFYWWVNFRQVARVLLREAGRTGATGFEAVTGSPLR
                     MNGINLLFAAVSCGPALIFGFVFWSQWDAYLRFSWGGPVGSIDPIFGLDIGFYLFRLP
                     FYEVLQNSLVGLTAVTLVLVFLAYYIFGLAGQRGASQYMPHWGATRHTGILFVLFLAA
                     WAWGYYLDRYELMFSSRGVVFGPGYADYNVVRIVLWIMLFVSAALGLFTAVSLLLKRF
                     RWILIGVGTCFALMIVLLYMLPFAIQKFVVRPNELELETPFLKHNIAYTRRAYQLDRV
                     EEKRYPALGDLTLKEISQNEDTIRNIRLWDWRPLRQTFRQT"
BASE COUNT         1053 a         1283 c         1535 g         1071 t
ORIGIN      
        1 gctgtggcgt cgatggtttg aacgcgggaa gcttccccgg agaagtctgc tgcggggaag
       61 cttcccgcgt tcaaaccatc gacgccacag cagacgaaca agccgaactc gacgtatgtg
      121 gtgaaacaga atcaggactc gcctgcgaga tcttcctgcc tggctaagtg cacggaggac
      181 atgaacaggt accgggcgaa atgtaacaaa ggtccggatc ctgttgaggc gtccaactgt
      241 gtcaaaatgg gcgagggtac gtataagtac tgtgcaggca agtgtccggc gaagtagggg
      301 aagcgcaccg ttatgaaggt ggagatatca acatatccct gccttgcgga agatactgaa
      361 gtatgagccg aagggagtct taagggggcg agttatcgga gatggtaccc gtgtccactc
      421 ctgctattgg cctccggtga atgttctcag cttgttcctg aagaacgctt cggattgaac
      481 ggttgtgccg tccgcgaggc gtatcaggta ggactcgcct gtcatagagg acctggaggc
      541 gcagaatttg atgaagtcct ccgctgtctg caccttgttg cccgccgcct tgagcttcag
      601 gcgcaggtgg gcagcggctg ccctggcatc atgctcgctt ccgttcctga tgaacgtcgc
      661 tccttccagt gcttcgacgg aggctaacag gtactggatc ctggccgtct ctgaaggatc
      721 cccggcacaa acacgcaccg ctgccagcaa gaggacggtc gcgagaaccg cggagatctt
      781 tttcatgtca aaggcacccc tgatctcaga atagcgcctc atggcgtggc gaaggaccgc
      841 ttccgatgaa accctggatc tggccgatgg gataataggt tttgtccggc ggcggcagtg
      901 acgcgctcag cgcgggaggt atcctgatga cctgtccgaa aacgggcgcg acgaaggcgt
      961 cgggtgccag accgtgttcc tttaatgatg cggcaagcac atccggggca tcggtaaatc
     1021 cctcgactcc cagccgaaat acctggaaat gggcggcgat gctgagcggt tctcccaggt
     1081 ccctgtgtgc catgaccgct tcggccgggc ccatgtggac aatggagtga tgatgatgct
     1141 gcgccgtcgc ctcgccggcc tcagggcgga atggggctat cggaaggagg gccaccctta
     1201 tcggtgaaaa gcggcgtgca atttcacgga aatggggacc atacccggtg tctccggcat
     1261 agtagacgtt ccctgatgga ccggatataa cgaaacctcc ccaaagagtc ttgtcccggt
     1321 cccagagggt gcgccccgag aagtgctggg cagggaccag ggttaccgtc actcctgaag
     1381 acaaagggac cgattgccac cagtcaagtt catccaccgc gggcattccc gtgctccgca
     1441 taagaccata attgccgagc ggcacgacgg cgcgcggtgt tcctttcttt gcaagacgtt
     1501 taagggtggg aatatcgaaa tgatcgtaat ggttgtggga taccagcacg acatcgacag
     1561 ggggaaggtc gccgaaacga atgccgggtt cgctgtgccg ttcggggccc acccatgata
     1621 ccggactgca gcgttccgac catatggggt cggtgagaat attgagtccg tccatctgta
     1681 tgagaaacgt ggcgtgaccg acgggcgtta cgtggatcga gccttcgggg gcgcgggcga
     1741 caggcggcgg tccaggcagg aactcttttt gctccggcca ctcgggccaa ccgacgcgaa
     1801 agatccactt ccaaacccac cacgtggcgc tgcgttttgt cgtctgaccc gacacagaat
     1861 acagggcctg gggagcaccg gggttaacat accgggcacc atcaaagtgg tccgagacct
     1921 gccgtgctcc tgcggcgggt atatccggtc ttgtcacgca ggcgggaagc gacagcaaag
     1981 aggccaggat aaaggacaag acaaggaggc acaggacatt ccttttgttt cttttcattc
     2041 aaactcccaa caaataaaca actcgtaaac gacaacccct cagaacatcg ggaccgaata
     2101 gtgtactata ttcccggagg caatgccagt tgttgcatct aggggtctcc gggggggcag
     2161 gataaagggg aatgaaatat ccagtctggt gatccggaag acggtagaga agaatcgaag
     2221 gtcccgtgag cagttcgggc caaccagatc atttttcatg acattcaggc cggcaggacg
     2281 gaagaggagg gtccagatcc ttgacgctga tgtccgcgta caggaaaaaa gtaaagatga
     2341 gaagattgca ggatctggag gagtctgcgc agagcgccga gcggctcgca cttgacatgg
     2401 gggagggctg gaattagatt gggccaatgg agccctggcc gcttatcagc tatgttgctg
     2461 cagctctggt ggtggtggac ctcatgtcga cctctcacat gttgttcgtc ggcggcaagg
     2521 agacaggtca acggaagagg cgcgcaaatc cggcatgttt ccgacggcga ctgcatctat
     2581 cattccatcc tgatcatctg gaaacaactc cacaacgaaa agacgaggct gaaaacgctt
     2641 gaggcggatg tcgggagagt ctcccggccc ttcgcgctcg atctcttgac gtagtgatac
     2701 ggcatcggtt ccacaggata ggttgtggga aacatgatca cgcgccacgt tctgaaagag
     2761 aggtccccga caaaccgcga tggcggtagc atgtcaggtg agtcgcgtcg gccgacggga
     2821 ggacggatgc catgaaaatt aacatcaatg agtacattca agagtttgtg gactggttcc
     2881 tgacgagcgg gctgcgtgtc gggatcacat tgatcctaac gttcgttgcc ctgaaggccg
     2941 cagggttttt cgcagcccgc ctgatcgcgg cccttagccg acacagagac gatgttgaat
     3001 accagaagcg gacgaggacc cttggttcga tcgtccggta tgtcctgaat acggccgtac
     3061 tgatcatcgc cgccatgatc gtgctcggag agttggggat agagataggt cctgttctgg
     3121 ctgcggcagg gatcgtgggg ttggcggtgg gctttggggc tcaaagcctg gtaaaggatg
     3181 ttatcagcgg gttcttcatt ctgatggagg accagatccg ggaaggggac gtcgttcaga
     3241 tcggcggaca gggaggactg gtcgagaaaa taagcctgag gaccacgaaa ctccgagacc
     3301 tggcgggcaa cgtgcattat gtgccgaacg gcaagatcga tgttgtcacc aacatgacaa
     3361 aggagtactc acgctatgtt tttgacatag gggttgcgta tcgagaggat gttgacgagg
     3421 tgatcggact gatcagggag gtcgacgaag agctgcgcgg cgatccggct ttcaaggatg
     3481 acattctgga gccgatggag gtactgggtc tcgatcagtt cggtgaatcg gctctcgtca
     3541 tcaaggcccg cacgaagacc ctgcctatca aacagtgggg tgttgctcgt gaattcaatc
     3601 gtcgtctgaa gatgaaattt gatgagaatg acgtcgagat cccatttccg cacgtcactt
     3661 tgtatatggg tcaggacaag aaaggccacg cgccgccctt gcacctcgca cgtgagggca
     3721 accccgggag atcggaggat gtctgaaagc gaaggacgtt tccagaccgt agcatttccg
     3781 ggcttcggtc accgcaaaga ccgggagaga tagccatgag actgatcgcg ggtaaaaaga
     3841 gggcgttctg gtgggtgttc cttgcccttg tcggtgtgtg cctgatcgtt ctgtccctca
     3901 ccgccggtaa gctcgtggag tggctatgga tgaagcagtt ggggtttgtc gggatcttct
     3961 ggcgattgtt gttcagcaag ctcctcctct ttgttgcggc ctttctcttc gttttcttct
     4021 actggtgggt caatttccgg caggttgccc gcgtgctgct cagggaggcg gggcggaccg
     4081 gcgcgaccgg tttcgaggcg gtcaccggat cgccgctgag gatgaacggg atcaacttgt
     4141 tgtttgctgc cgtgtcctgc ggacccgccc tgatattcgg ttttgtcttc tggtcgcagt
     4201 gggacgcata ccttcggttc tcctggggtg ggcccgtcgg cagtatcgat ccgatcttcg
     4261 gactcgacat cgggttttac cttttccgcc ttccattcta tgaggtcctt cagaacagcc
     4321 tcgtcgggct gaccgcggtc acgctggtgc tcgtcttcct tgcctattat atcttcggcc
     4381 ttgccgggca gaggggagcg tcgcagtata tgcctcattg gggcgccacg cgtcatacgg
     4441 ggatcctttt tgtactgttt ctggcagcat gggcatgggg ctattacctg gaccgatacg
     4501 agttgatgtt ttccagcaga ggcgttgttt tcggaccagg ctatgccgac tacaacgtgg
     4561 ttcggatcgt tctctggatc atgctcttcg tgtccgcggc cctggggctc ttcaccgccg
     4621 tttccctctt gctgaaacgg tttcgatgga tattgatcgg ggtaggcacc tgctttgccc
     4681 tcatgatcgt gttgctgtac atgctgccct tcgcgataca gaagttcgtg gtgcggccca
     4741 acgaactgga gctcgagacg ccgttcctga agcataacat cgcgtatacg cgcagagcct
     4801 atcaactgga tcgggtcgaa gagaaacggt atcccgccct cggcgacctc accctgaaag
     4861 agatatcgca gaacgaggac accatacgga acatcagact gtgggattgg aggcctctcc
     4921 gccagacctt ccgtcagacc ca
//
LOCUS       DAOQTR010000091         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_19153_length_4894_cov_4.596818, whole genome shotgun sequence.
ACCESSION   DAOQTR010000091 DAOQTR010000000
VERSION     DAOQTR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19153_length_4894_cov_4.596818"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKN85_03255"
     CDS             <1..383
                     /locus_tag="PKN85_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNT43450.1"
                     /translation="IALAKRLGGKIVTNDYNLNKVAELQGIEVLNMNQLAMSLRPALL
                     PGEQMNIKVLREGKEQGQGIGYLDDGTMVVVDDARKYLGKAVDVVVTSVLQTTSGRMI
                     FSRLKGEAGKDYIYPDEYQLEEQA"
     gene            391..1089
                     /gene="ispD"
                     /locus_tag="PKN85_03260"
     CDS             391..1089
                     /gene="ispD"
                     /locus_tag="PKN85_03260"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNT43451.1"
                     /translation="MRTIAIILAGGAGKRMGAATNKQFLLIENKPIIVHTLQIFEDCR
                     AVDGIYFVVNQKDLPLIQEEILETYKFNKIMKIVMGGKLRQDSVRNGLEAIEHPCDIV
                     VIHDGARPFVSPAFIEKGIFLMEMYDAVIPALPVKDTVKVISKEGFVVKTLERDSLWH
                     VQTPQTFKYRLIMKAYREGMAKRHYGYDDATFIEHLGKKVKVIEGSPYNMKITTPEDL
                     TIARGLLSQLKGTL"
     gene            1086..1559
                     /gene="ispF"
                     /locus_tag="PKN85_03265"
     CDS             1086..1559
                     /gene="ispF"
                     /locus_tag="PKN85_03265"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNT43452.1"
                     /translation="MRIGTGFDVHRLTDGRRLVLGGVEVPFGKGLLGHSDGDVLLHAI
                     SDAILGALSEGDIGVHFPDTDKEIEGIDSAVILARICAIARDRGYRVVNVDSVVVAQA
                     PKIAPYREAMRERIAGLLSIDAGMVGLKGKTTEGLGLTGRGEGIAAHAVVLMDRV"
     gene            1602..2966
                     /gene="gltX"
                     /locus_tag="PKN85_03270"
     CDS             1602..2966
                     /gene="gltX"
                     /locus_tag="PKN85_03270"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNT43453.1"
                     /translation="MVRVRFAPSPTGHLHVGNAKTALFNWLFARKNGGSFVLRMEDTD
                     VERSDVAYERSITEDLAWLGITWDEGPLRQSERLHIYTSHAKTLLEKGSAYRCFCGEE
                     ELAAARQRSLARGEPPRYDGKCRDLSTGEALRLAEEGRPFTLRFRSDGRPITFRDGIR
                     GELSFPRGHVDDLIILRRDGTPTYNLAVVVDDMLMGITHVIRGGDHISNTPKQIALIN
                     ALGGKPPEYAHHSLLIGPDRKPLSKRHGATGVTDFRAMGIVSEALVNYLSTMGRSVAV
                     ELMDPDELVRTFSLDSVSGSDNIFDMEKLLWFNREHMKRMTLSHLADRAGIDASRRER
                     VSVVRENAATLTELREYMEMFDRVEFRPEALRYLMKTDAAGGVAKTVRSRLAAGDSLT
                     FDELTAVLGSPPGLKKRDLFMILRALITGRSDGPPLRDVFPLIPAEVIAGRIDVYLEH
                     GHEG"
     gene            2935..4476
                     /locus_tag="PKN85_03275"
     CDS             2935..4476
                     /locus_tag="PKN85_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43454.1"
                     /translation="MSTSSTDTKDSGRMEVGGRRGMTGKAGKVYALVKSVFRSLSLRT
                     QLLLILLFLLLISIGSLTIIYTRAEEALLDKVGENIDDITRAIQISVEELTYRGDSTA
                     RLKSYVDMLNKKGIKEISIVSEKSEVIASSDPKKIGTTQKLTDKKPAARKKDLMITAR
                     LGDEHRSGTQRLYNIIMPVAIKGQNIGYIHINMALDDYRLLQNRNQIKRILSMIFAFT
                     IGIIVCLVIAEKYTEPIKRIAKASRKIAMGELVKIRETDRKDEIGTLVTSFNEMVDKL
                     DERKELEEKLKKSEQLSLIGQLSSGIAHEIRNPLNFLTLSIGHIKERVTEEPISDKEE
                     LVGLLDSLLKEIYRVNELIHNFLFLGKSITLVREDVPPANLVDEALVMVKDKIRSGID
                     IELSCSDGAGTMYCDREYMRLCVINLVINAVQAIQGPGRVRIECGTEDSVTYLSVSDD
                     GEGIARDELDKIFEPYYSTKKLGIGLGLAITKRFVEEHGGTISAASEIGKGTTMTIRL
                     PRHEG"
     gene            4466..>4894
                     /locus_tag="PKN85_03280"
     CDS             4466..>4894
                     /locus_tag="PKN85_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43455.1"
                     /translation="MKDDTKGTILVVEDDRNQRGIIKTILSKEGFYVEAAECGKKAVD
                     ILKNGSFDVVLTDLKLPDIDGTEVLREVRALGRTCHVIIMTAFGSIPSAVEATKLGAF
                     YYLEKPLEKDQLLLVITNAMNQVKLLKDNIMLKDQLRDRFH"
BASE COUNT         1129 a         1405 c         1506 g          854 t
ORIGIN      
        1 ttatagccct cgcgaaaagg ctgggaggga agatcgtcac caacgattac aacctcaaca
       61 aggttgccga gctgcaaggc atcgaggtcc tcaacatgaa ccagcttgcc atgtcgctcc
      121 ggcccgcgct gctgccgggc gagcagatga acatcaaggt gctccgcgaa ggcaaggagc
      181 agggccaggg gatcggatac ctcgacgacg ggacgatggt ggtggtcgac gatgccagga
      241 aatacctggg caaggccgtc gacgtcgtgg tcacgagcgt cctgcagacg acctcggggc
      301 gcatgatctt ttcgaggctc aagggagagg cggggaagga ttacatctac cccgacgaat
      361 atcaattgga agaacaggcg tgataccgac atgagaacaa tcgcaatcat tctcgcaggc
      421 ggcgcgggaa agaggatggg tgccgccacc aacaagcagt tcctgctcat cgagaacaag
      481 cccatcatcg ttcacacact ccagatattt gaagactgcc gggccgtgga cgggatctat
      541 tttgtcgtca accagaaaga cctccctctc atccaggagg agatactgga gacctacaag
      601 ttcaacaaga tcatgaagat cgtcatgggc ggcaagctca ggcaggactc ggtgaggaac
      661 ggactggagg ccatcgagca cccctgcgac atagtcgtca tacatgacgg cgcgcggcct
      721 ttcgtatccc ccgcgttcat tgaaaaaggc atctttctca tggaaatgta cgatgcggtc
      781 ataccggccc tgcccgtgaa ggacaccgtg aaggtcatct ccaaggaagg gttcgtcgtg
      841 aagaccctcg agcgggactc gctctggcac gtgcagaccc cccagacgtt caaataccgc
      901 ctcatcatga aggcttaccg ggaggggatg gcgaaaagac actacggata tgacgacgcc
      961 acgttcatcg agcacctggg aaagaaggtg aaggtcatag agggttcccc ctacaatatg
     1021 aagatcacca cacccgaaga cctgaccatc gcgagggggc tcctttcaca gctcaaaggg
     1081 accttatgag gatagggaca gggttcgacg tccatcggct gacggatggg agacggcttg
     1141 tcctcggggg tgtcgaggtt ccattcggga agggccttct cgggcactcc gacggcgacg
     1201 tcctcctcca cgccataagc gacgcgatac ttggcgcact ctctgaagga gatatcgggg
     1261 tccacttccc cgacaccgac aaggagatcg agggcatcga cagcgccgtg atacttgcca
     1321 ggatctgcgc gatcgcccgc gaccggggct accgggtcgt caacgtcgac agcgtcgtcg
     1381 tggcacaggc gccgaagata gccccgtacc gggaggcgat gagggagagg atcgccggtc
     1441 tcctgtccat tgacgccggc atggtgggcc tgaagggcaa gacgacggag ggtctcgggc
     1501 tgaccggcag gggcgaaggt atcgccgcgc atgccgtcgt tctcatggac agggtctgac
     1561 agaggtatgt gtcccgcttt tccacgacat gtgaggcgaa gatggtcagg gtaaggttcg
     1621 ccccgagccc gacggggcat ctccatgtgg gaaacgcgaa gacggcgctc ttcaactggc
     1681 tctttgcaag gaagaacggg gggtccttcg ttctgcgcat ggaggatacc gacgtcgaac
     1741 gctccgatgt cgcctacgag aggtccatca cggaggacct cgcctggctc gggatcacct
     1801 gggacgaagg accgctgcgc cagtcagagc gcctccacat ctatacgtcc cacgcgaaga
     1861 cccttcttga aaagggatcc gcgtaccggt gtttctgcgg cgaagaagag cttgccgcgg
     1921 cgaggcagag gtcgctcgcg cggggcgagc ccccgaggta cgacgggaag tgccgggacc
     1981 tctccacagg ggaggccttg aggctagcgg aagaggggag gcccttcacc ctgcgcttcc
     2041 ggtcggacgg gaggccgatc acgttccgcg acggcatacg gggggagctt tctttcccga
     2101 gaggccacgt ggacgacctc atcatcctca ggcgggacgg taccccgacg tacaacctgg
     2161 ccgtcgtcgt cgacgacatg ctcatgggga tcacccacgt catacgggga ggggaccaca
     2221 tctccaacac gcccaagcag atagccctca tcaacgccct ggggggcaag ccgccggagt
     2281 acgcacatca ctctctcctc ataggtcctg accgcaaacc cctcagcaag aggcacggtg
     2341 ctacgggcgt tacggatttc cgtgccatgg ggatagtgag cgaggcgctt gtgaactacc
     2401 tgtcgacgat gggtcggagc gtcgcggtgg agctgatgga cccggacgag cttgtccgga
     2461 ccttttccct tgactccgtc tccggttcgg acaatatttt cgatatggag aagcttctct
     2521 ggttcaacag ggagcatatg aagcgcatga cgctttccca cctcgccgac agggcgggga
     2581 tcgatgcctc ccgtagggag agggtgtccg tcgtgcgcga gaacgcggcc acgctcaccg
     2641 agctcagaga atacatggag atgttcgaca gggtggagtt caggccggaa gcgcttcgtt
     2701 acctcatgaa gacggacgcg gccggagggg tcgcaaagac cgttcggtcc cgcctcgcgg
     2761 ccggggacag tctgaccttc gacgagctca ccgccgtcct cgggtcaccc cccggcctga
     2821 agaaaaggga cctcttcatg atcctgaggg ctctcatcac gggaaggtct gacgggcctc
     2881 ccctgcggga cgtcttccct ctcattcccg cggaggtcat tgcaggtcgc atcgatgtct
     2941 acctcgagca cggacacgaa ggatagcggg cgcatggaag tgggcggcag gcgcggcatg
     3001 acgggaaagg cggggaaagt ctacgcactc gtgaaatccg tttttcgcag cctgtcgctg
     3061 aggacgcagc tccttctcat ccttctgttt ctccttctca tatccatcgg ctcgttgacc
     3121 atcatctaca cgagggccga agaggcgctt ctcgacaagg tgggcgaaaa catcgacgac
     3181 atcaccaggg ccatccagat aagcgtcgag gagctgacat accggggtga ttccacggcc
     3241 cggctcaaga gctatgtgga tatgctcaac aagaagggga tcaaggagat ctccatcgtc
     3301 agcgagaagt cggaggtcat cgccagttcc gacccgaaga agatcggcac cacccagaaa
     3361 ctgacggaca agaagcccgc cgcgcggaag aaggacctta tgatcacggc gcgcctcggg
     3421 gacgagcatc gcagcggcac acagcggctc tacaacatca tcatgcctgt cgcgatcaag
     3481 ggccagaaca tcggctacat ccatatcaac atggcgctcg acgactaccg tcttctgcag
     3541 aaccgcaacc agatcaagag gatcctcagc atgatcttcg ccttcaccat cggcatcatc
     3601 gtgtgcctcg tgatagctga gaaatacacg gaaccgatca agaggatcgc caaggcaagc
     3661 aggaagatcg ccatgggaga gcttgtcaag ataagggaga cggaccgcaa ggatgagatc
     3721 ggcaccctgg tgacgagctt caacgagatg gtggacaagc tcgacgagcg caaggaactg
     3781 gaagagaagc tgaagaaatc ggagcagctt tcgctcatcg gccagctctc ctcgggcatc
     3841 gcccacgaga tacggaaccc cctcaatttc ctgaccctct ccatagggca catcaaggaa
     3901 agggtgacgg aggagcccat ttcggacaag gaagagcttg tcgggcttct cgacagtctt
     3961 ctcaaggaga tataccgggt gaacgagctc atccacaact tcctcttcct cgggaaatcg
     4021 atcacgctcg tgagggagga tgtgcccccg gcgaaccttg tcgacgaggc gctcgtcatg
     4081 gtgaaggaca agatacggag cggcatcgac atcgagcttt cctgcagcga cggcgccggc
     4141 acaatgtact gcgacaggga atacatgcgg ctctgcgtca tcaacctcgt gataaacgcc
     4201 gttcaggcca tacaggggcc cggaagggtg cgcatcgagt gcgggacgga ggattccgtg
     4261 acgtaccttt cggtctccga tgacggggag ggtatcgcga gggatgagct tgacaagatc
     4321 ttcgagccct attactctac aaaaaaactg ggcattggcc tcggtctcgc cataacgaag
     4381 cggttcgtcg aagagcatgg cgggaccata tcggcagcga gcgagatcgg aaagggaacg
     4441 acaatgacca taaggcttcc acgccatgaa ggatgacacg aagggcacca tccttgtcgt
     4501 cgaggacgac aggaaccagc gcgggatcat aaagacgatc ctctcgaaag agggtttcta
     4561 cgtcgaggcc gccgagtgtg gaaagaaagc ggttgatatc ctcaagaacg gcagtttcga
     4621 tgtcgttctc acggacctca agctccccga catcgacggc acggaggtgc tgagggaggt
     4681 gagggcgctg gggaggacct gccacgtgat catcatgacc gccttcggct ccataccctc
     4741 ggcagtggag gcgacgaagc ttggcgcctt ctactacctc gagaagcccc tcgagaagga
     4801 ccaacttctc ctcgtcatca cgaatgccat gaaccaggtg aagctcctca aggacaacat
     4861 catgctgaag gaccagctgc gggaccgctt tcac
//
LOCUS       DAOQTR010000092         4892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_19163_length_4892_cov_3.913376, whole genome shotgun sequence.
ACCESSION   DAOQTR010000092 DAOQTR010000000
VERSION     DAOQTR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19163_length_4892_cov_3.913376"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKN85_03285"
     CDS             <1..742
                     /locus_tag="PKN85_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43456.1"
                     /translation="DIAEMSGAQVWATENTARWLENYDDECLEEERFQKTFLDRAGVP
                     ADLVAHVGRYFQYLKKFACPVRVSRLLEVGEVVELASGRFRLEHVPGHTPWCVMMVDD
                     REKIAFTGDFLLKDISSNPLAQRPSKLPAGYRSLEAYASSLRRVAAMGLALALPGHGE
                     MMRNPRKRVAALLGFMNARTGQVADIFGTRPDLTIYEIVEEVFPGLPRDEIFLAVSEV
                     FDHLDLLEGNGIVLRTDEGIPSRYTLCR"
     gene            1012..1341
                     /locus_tag="PKN85_03290"
     CDS             1012..1341
                     /locus_tag="PKN85_03290"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HNT43457.1"
                     /translation="MKGPFSKIVLVAVCLCLLCTFAGATTSDTFRCPNGAIISVNDRL
                     STVTLRCDTPTSVSRRSVTRGLASGYYDVVEIEEWTYDLGPSLFVYYLTFENGVLVRI
                     ESGGYGK"
     gene            1527..2408
                     /locus_tag="PKN85_03295"
     CDS             1527..2408
                     /locus_tag="PKN85_03295"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNT43458.1"
                     /translation="MIKGTTKILGVVGYPIVHSLSPVMHNAAIEHLGADYVYVPFSVK
                     PEDLAVALRGFEAIDVQGFSVTIPHKQAIMPLLTEITAEAKLSGAVNAVSRTESGWKG
                     TNYDSFGFMAPLKEMKENWKSIAPVILGNGGAARSVLVGCQSLGCPEVHVFGRNPQKL
                     ADFADSWKNTPVHDVLRVHAWDELGGMISGTRLVVNATPMGMYPRGDQLPVTEDVIGG
                     IQPGTVVYDLIYSPRPTLFLSKARERGAVIVDGLEMLVQQGAKALAIWLGRDVPVEIM
                     RDACLKQSKPAGPGSGT"
     gene            2746..3837
                     /locus_tag="PKN85_03300"
     CDS             2746..3837
                     /locus_tag="PKN85_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43459.1"
                     /translation="MGLIDKVKLKVYPVVSRYISHATLVEGRLGNGGETFRCLFVENT
                     DLMGYMLPRMYDDRPAVLRKWRIWTPSLPRVLRDHSNSIDMCVAVLPLSYEPKFEKSA
                     QFRSQMLICSFMDMSGGWTEIKKRFQHNKRQFSNRMDKRPTFSCRVSKDQKDFELFYN
                     EMYAPHIQKRFEDLADIDSYSHMKDSFDKGFLLVIEEGDTCVAGVLCEVQNDTLLARR
                     TGILHGDEEYIRRGAASAEYYFMLKFALEHDLSRVDLLRSRPFFNDGVYSTKRKWGAT
                     VYPDRESRSWVFFLIPEYSRKVASFFQINPVIVCRDDKMYGLVGWNNEDAPSEKDAKE
                     FSQKYYSPGLDGLMLIRPHCEEPVQIPFR"
     gene            4387..4587
                     /locus_tag="PKN85_03305"
     CDS             4387..4587
                     /locus_tag="PKN85_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43460.1"
                     /translation="MPAILIMAGLGGCFVAYEKPGMTQAEWNRDKAYCDQVAEKEYVR
                     KGTRICDEVDACLIAKGWKRSQ"
     gene            4669..>4892
                     /locus_tag="PKN85_03310"
     CDS             4669..>4892
                     /locus_tag="PKN85_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNT43461.1"
                     /translation="MEISDKELRTILRRHRKWLDGEDDGICANLSGADLSGARLEGAD
                     LSHANLRSADLSGATLVRANLSGVDLSEADL"
BASE COUNT         1029 a         1270 c         1530 g         1063 t
ORIGIN      
        1 ggacattgcg gagatgtccg gggcgcaggt atgggccacg gagaataccg cccggtggct
       61 cgagaattac gacgatgaat gcctcgagga ggaacgattc cagaagacct tccttgatcg
      121 tgccggcgtg cccgcggatc ttgttgccca tgtcgggcgg tatttccagt acctgaagaa
      181 gttcgcctgc cccgtcaggg tgtcccgtct cctggaggtc ggggaggttg tcgagcttgc
      241 ctcgggccgc ttcaggctgg agcacgtgcc cggccacacg ccgtggtgcg tcatgatggt
      301 cgacgacagg gagaagatag ccttcacggg agatttcctg ctgaaggaca tatcgtcgaa
      361 ccctctggcc cagagaccgt cgaagctccc cgccgggtac cggagtctcg aggcctatgc
      421 gtcatcgctg aggcgcgtgg ccgcgatggg tctcgcactc gccctgccgg ggcacggaga
      481 gatgatgagg aacccccgga agagggtagc ggccttgctg ggtttcatga acgcgagaac
      541 aggacaggtc gcggacattt tcggaacgag gcccgatctc acgatatacg agatcgtcga
      601 agaggtattc cccgggcttc cgcgggatga gatctttctg gccgtctcgg aggtctttga
      661 ccatctcgat cttctcgaag ggaatggtat cgttttgcgc accgatgagg gtattccttc
      721 ccgctacacc ctgtgtcgct agaagacacc ggggcgcggc gtcaccggcg tgccagggcc
      781 ggagcgtcct caatcagtcg caacgtgagg ggtcagtcat tgaagagccg tttccggcct
      841 ctgattgaca cttttgtatc tctgagatag tattgctgat atgcagatgc gtgccgcaca
      901 cctgtgatca tcacaaggag cccacatgga agacccctcc gaaacgtatc ggcagatgat
      961 gatgcacccc tggatatgtt tcacaggggc ggagtaaaag ggggaataag gatgaaaggt
     1021 ccattctcaa agatcgtgct tgtcgcagtc tgcctttgcc tgctgtgtac tttcgcgggc
     1081 gcgacgacgt cggacacctt caggtgcccc aacggcgcca tcatctccgt caatgacagg
     1141 ctctcgacgg tcacgctcag gtgcgacacc ccaacctccg tctcacggag gtccgtgacc
     1201 agaggacttg cctcagggta ctatgacgtg gtcgagatag aggagtggac gtatgatctg
     1261 ggcccgtccc tcttcgtata ttaccttacg tttgagaatg gggttctcgt gcgcatcgaa
     1321 agcggaggtt acgggaagta gtctatttcg cttccgggag catggttccg tgcggttcgt
     1381 ttgtgatggg accgcgagaa cggggaaagg tgcgccggag gggtgccaca taaaccttga
     1441 catgcgcggt acggtgcggg atgattttgt tgtttcaact ggttcgtcag cggttgcaca
     1501 tacagaatcg gatcctggtg gtggtgatga tcaaaggaac gactaagata ctgggggttg
     1561 tcggctaccc cattgtccat tcactctctc ccgtgatgca caacgctgcg atagagcatc
     1621 ttggagccga ctatgtctat gtcccctttt cggtcaagcc cgaggacctg gccgttgccc
     1681 ttcgcgggtt tgaggccatc gatgtgcagg ggttcagcgt taccattccc cacaagcagg
     1741 ccattatgcc gctcctgacg gagatcaccg ccgaggcgaa gctgtcgggt gcggtgaacg
     1801 ccgtttcccg gacagagagc ggctggaagg ggaccaatta cgattccttc gggtttatgg
     1861 cccctctgaa ggagatgaag gaaaactgga agagcatcgc ccccgttatc ctggggaacg
     1921 gaggagcggc gaggtcggtc ctcgttggct gccagtcact gggctgcccc gaggtccacg
     1981 tcttcggacg gaacccccaa aagctggccg attttgcaga tagctggaag aacacgcccg
     2041 ttcacgatgt cctcagggtc catgcctggg atgaactggg aggtatgata tccgggacgc
     2101 gacttgtcgt caacgccact ccgatgggca tgtatcccag aggggaccaa ctccccgtga
     2161 ctgaggacgt gatagggggt atccagccgg ggaccgtggt ctatgacctg atctattcac
     2221 cccggcccac tttgttcctg agcaaggccc gcgagagggg agcggtcatc gttgacggcc
     2281 tggagatgct ggtccagcag ggggcgaagg ccctggcgat atggctgggc cgggatgttc
     2341 ccgtggaaat catgcgggac gcctgtctga aacaatcaaa acctgccggc ccgggaagcg
     2401 ggacctgaca cgaaggtcct gagagcggct cgcgacaggg acacggatct cgcttcggtt
     2461 gctcttttct tccgacctgc cgtgcaagcg acaaacccat aagtctgcct tgtgtttgtt
     2521 acgaaccacc tcgtggtatc atatcggccg attgttccgt ggcgaagact ttgtcccaaa
     2581 catagcattg acgggccttt gagggcggtg accgtggagg cccatcgttg acagtcgact
     2641 ccggtctgct atatttcatg ttaagcggag actgtgccct gcggtgcttg ccgcgggtct
     2701 cttgtcgatt caacagcccg gttccgggga agggggaaga caactatggg actcatcgac
     2761 aaggtcaagc tcaaggtcta tccggtggta tcccggtaca tcagccatgc aactctcgtc
     2821 gaaggaaggc tcggaaacgg gggtgagaca ttccgctgcc ttttcgtgga gaacacggac
     2881 ctcatggggt atatgctccc tcggatgtac gatgaccgcc ctgctgttct gaggaagtgg
     2941 cgcatctgga cgccctcttt accgagggtt ctgagagatc actccaactc gatcgacatg
     3001 tgcgttgccg tgcttcctct gagctatgag ccgaagtttg aaaagtcggc acaattcagg
     3061 agccagatgc tcatttgctc tttcatggat atgtcgggcg gttggactga gatcaagaaa
     3121 cggtttcagc acaacaagag acagttctcc aacagaatgg acaaaaggcc aacattctct
     3181 tgccgggtct cgaaggacca gaaagacttc gagctcttct acaatgagat gtacgcgccc
     3241 cacattcaaa aaaggtttga ggaccttgcg gatattgact cctacagtca catgaaggac
     3301 tccttcgaca agggttttct cctggtcatc gaggaagggg acacatgtgt cgcgggtgtg
     3361 ctctgcgaag tacagaacga caccctgttg gccagaagga cgggaatcct gcacggagac
     3421 gaggaataca tcagaagagg cgccgcgtct gccgagtact atttcatgct caaatttgcc
     3481 ctggagcatg acctctcgag ggtagacctg ctcagaagca ggcctttctt caacgacggt
     3541 gtttacagca ccaaacgaaa atggggcgct acggtctatc ccgaccggga atcccggtcc
     3601 tgggtttttt tcctcattcc cgagtattcc agaaaggtgg caagcttttt ccagatcaat
     3661 cctgtgatcg tttgcaggga tgacaagatg tacgggttgg tcggctggaa caatgaagat
     3721 gctccgtccg agaaagatgc aaaggaattc agtcaaaaat actattctcc cggtctcgat
     3781 ggcttgatgc ttatccggcc tcattgtgaa gaacctgtgc agatcccgtt cagatgaatc
     3841 cctggagggg ttgcgggaat cgtttgggac aaggactgtc tttgcgtgtg agaccgccgg
     3901 aatactgtct ttgccgcagg gagggctgtt gacaagtatc cgggtggcgc catgttttta
     3961 ttatgggggc cggcgagttg ggtaagcgtc gccggtatga ttccagcgag tgccgaaggc
     4021 gcacatgaag atcacgtgat gcgcgaacct gtgggcattc tccgggacac ttcttccgta
     4081 tctgtggtct gtcaatcttt atcatttcct tggccccggg gttagggggg atgtcggcac
     4141 gccggttatc tatcctgctc cgggagtacg ggggcatggc ggggcggcaa cggcccgact
     4201 tctgcggacg gttggttgct cgtttgtccc ggtcagatta tcccttgtaa attggtgttc
     4261 attgcgggaa aatggagatt ggaatgctgt gggcgaagcc ggaaacatcg tcttgatgat
     4321 agcaccaaag acaaggggga aactgggatg atgtcatgcc ttaggagggt aaaagggaaa
     4381 tggttggtgc cggctatcct gatcatggcc ggccttggcg gctgcttcgt ggcctacgag
     4441 aaacccggga tgacccaggc ggagtggaac agggacaagg cgtactgcga ccaggtcgcg
     4501 gaaaaggaat acgttcggaa aggcacccgg atctgcgacg aggtcgatgc ctgcctgata
     4561 gccaaggggt ggaagagaag ccagtaggac agggtatcca caggccgagg ggaaaggggt
     4621 tcaacctatc gggctggatg cattctacac tgacacgggg aacaattcat ggagatcagt
     4681 gataaggaac tcaggacgat attgcggcgg cacaggaagt ggctggacgg cgaggacgac
     4741 ggcatttgcg ccaacctctc cggggccgat ctgtccgggg ccaggctgga gggcgcggac
     4801 ctgtcgcacg caaaccttcg ctcggcggac ctgtcgggtg caacgcttgt cagggctaac
     4861 ctatccggcg tcgacctctc cgaagcggac ct
//
LOCUS       DAOQTR010000093         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_19222_length_4883_cov_3.671292, whole genome shotgun sequence.
ACCESSION   DAOQTR010000093 DAOQTR010000000
VERSION     DAOQTR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19222_length_4883_cov_3.671292"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..531
                     /gene="mlaD"
                     /locus_tag="PKN85_03315"
     CDS             43..531
                     /gene="mlaD"
                     /locus_tag="PKN85_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNT43462.1"
                     /translation="MKKYSIETTVGIFIVAGLICVGYMTVKLGNVSLFGEKTYPLKAR
                     FTSVAGLRVGSAVEVFGIQVGSVTSLTIDQERQMGVVGMKIDKGTVIYDDASAIIKTA
                     GLIGDKYVKVDPGGSGEVLKPGGAITQTSVPADIEDLIGKYAFGDVKKPPAQGEEQPP
                     AK"
     gene            546..1136
                     /locus_tag="PKN85_03320"
     CDS             546..1136
                     /locus_tag="PKN85_03320"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT43463.1"
                     /translation="MRKVLAGLMIFIISMIPLHAFAASALDMVKTNANSVLDVLRDPK
                     LKGDAGKKVKEQKIEAAAEKLFDYVELSKRTLGLNWNRLSMDQRKEFVDLFKTLLRNT
                     YIDRITAYTNEKVEFTKEMQLTETTTEVQSVVTKGNTTVPINYRVIKKGNDWMVYDVV
                     IEGVSLISNYRTQFRDILGSNPPQVLIDTLRKRVGK"
     gene            1448..2167
                     /locus_tag="PKN85_03325"
     CDS             1448..2167
                     /locus_tag="PKN85_03325"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNT43464.1"
                     /translation="MTAEDVGEAGAAAKESADEYGDVAPDSATAAKPQVQIADPLRPF
                     NVAMFHFNDKLYFWMWKPAATGYKYVMPQPFRQLFSNFYRNLTAPVRILNNLFQGKPG
                     YAGEELAKLLINSTVGVGGLRNCAEECFGIKGRNADFGQTLGKWGLGPGFYLVLPFLG
                     PSSPRDGFGFLVDWALRPQTYVGTEFFDYQNVGLFVHEKINSTSFRIGEYEALKKAAV
                     DPYVSMRDIYIQYRTDLIENR"
     gene            complement(2182..2637)
                     /locus_tag="PKN85_03330"
     CDS             complement(2182..2637)
                     /locus_tag="PKN85_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43465.1"
                     /translation="MKKIDALAETFAGSWGRFVHDFVGIVVLKVEDVARSKQASGQAP
                     GPYEARLIPLVEKIAEDIHEDQGSLLQAVHAELNEDPASWPVLSYLVREFEFFHELTS
                     LSRGNDVDEAIEAGRTIRGSFEEVVAMPGKVRKSLKVLDGVLELLKRAA"
     gene            2797..3444
                     /locus_tag="PKN85_03335"
     CDS             2797..3444
                     /locus_tag="PKN85_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43466.1"
                     /translation="MSLLFIQYWDVIQGREKEYTDYLNNTYLAEVTTLGLVPVGGYFV
                     EVGFGPRTITAFSSESLEDISRIITGKEFRDLALGLRKYVANFNNTVLEPDGTVKLGK
                     YPVQKGVWKFNQYYDLKPDMKEGYQDFIVNEYLPAMAKIDYVTVTNCWNVVLGGFCDI
                     VLEITFKDPEDIGHLLKNNDLQRITRRLIREFVTNYTNRIQRTTKWFSDPRWFRR"
     gene            complement(3467..3730)
                     /locus_tag="PKN85_03340"
     CDS             complement(3467..3730)
                     /locus_tag="PKN85_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT43467.1"
                     /translation="MYTLQFLGKAVEDLKGIDFPFQRIIKEKLLILAENPDVLKNNIT
                     RLSGTDEEYYRLRVGNYRIIFEKKERELLILVVRIGHRREVYR"
     gene            complement(3734..3958)
                     /locus_tag="PKN85_03345"
     CDS             complement(3734..3958)
                     /locus_tag="PKN85_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43468.1"
                     /translation="MQTRHNITLSEDIARELDSVAGELGEKKSSVIEKALMVYFDLLD
                     LRVAQKRMKDLKEGRDRTVDARDVWKEIGI"
     gene            4161..>4883
                     /locus_tag="PKN85_03350"
     CDS             4161..>4883
                     /locus_tag="PKN85_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT43469.1"
                     /translation="MRYDFDKAVEREGTDSIKWRRYGGGVLPLWVADMDFVSPEPVVR
                     ALHERVDHRVFGYGGATEEFVEVIRERLKRLYGWDVQGEEIVLVPGVVTGLNLAFTLF
                     AEPGEAVLVQPPVYSHFIADPVNRGRTVIDPPLVKRGDTYEIDFDAFEEAITDRTRIF
                     VLCNPHNPVGRVFRKEELERLADICLRRDVLICSDEIHCDLLFPGNRHLPIATLGNEV
                     ADRTITFMSPSKTFNLAGLKCSF"
BASE COUNT         1094 a         1330 c         1450 g         1009 t
ORIGIN      
        1 gatcatatcg acataccggt gtgattttgg aggtgaggag caatgaaaaa gtattccata
       61 gagacgacgg tgggcatttt catagtggca ggtctcatct gtgtcggcta tatgaccgtg
      121 aaacttggca atgtgtcgct cttcggagag aaaacctatc ctctcaaagc ccgcttcacc
      181 tcagtcgcgg gactgagggt gggaagcgcc gtcgaggtat tcggcatcca ggtgggctcg
      241 gtgacgagcc tcaccatcga tcaggagagg cagatgggtg tggtggggat gaagatcgat
      301 aaagggacgg tcatctacga cgatgcgtcg gccattatta agaccgcggg ccttatcggc
      361 gataaatatg taaaggtgga tccgggggga tcgggagagg tcctcaagcc cggaggagcc
      421 atcacccaga cctcggttcc ggcggatatc gaagacctca taggcaagta cgccttcgga
      481 gacgtcaaga aaccgcccgc gcagggtgag gaacagccgc ctgccaaatg atcaaggagg
      541 aataagtgag aaaagtactg gccggtctga tgatattcat catatcgatg atacctctcc
      601 atgcctttgc ggcctcggcc ctggatatgg tcaaaacgaa cgcgaacagc gttctcgacg
      661 tgctcaggga cccgaagctc aagggggatg cgggtaagaa ggtcaaggag cagaaaattg
      721 aggcagcggc tgaaaagctc ttcgattatg tggaactttc gaagcggacc ctggggctta
      781 actggaacag gctgagcatg gaccagcgca aggagtttgt cgatcttttc aagacgctcc
      841 tcaggaacac ttatatagac aggattacgg catacacgaa tgagaaggtc gagttcacga
      901 aggagatgca gcttaccgag accacgacgg aagtgcagag cgtggtcaca aagggcaaca
      961 ccacggtgcc catcaattac agggtcataa aaaagggtaa cgactggatg gtatacgacg
     1021 tcgtcattga gggggtaagc ctcatcagca attaccgcac gcagttcaga gacatcctgg
     1081 gaagcaaccc tccgcaggtg ttgatagaca ccctgcggaa aagggtaggg aaatgatgtc
     1141 gaggcgccgc tctgccgccg ggcagccccg aaggcgaatc tctttgagga ccatgggcgg
     1201 actctgtctg accatggcgt ttcttctgtg ctgccccttg ctccacgcgt aggagggcat
     1261 tccggaactc ggcaagatca cgatacggga ccttcaggac acgggggaca ggccggcgtc
     1321 acgtgccgct tacaccatgg cggccggcaa cacatcctgg agaggccttc aggcgaccga
     1381 aggcgaacgc gaaaattttt cgactttcgc cgccgccaga cagacgccgc cggatgaata
     1441 cggtgatgtg acagcggaag atgtgggtga agccggtgcc gccgcgaaag aatcggcgga
     1501 tgagtacggt gatgtggcgc ccgacagcgc gaccgcggcc aaaccgcagg tccagatagc
     1561 cgatcccctg cggccattca acgtggccat gtttcacttc aacgacaaac tctatttctg
     1621 gatgtggaaa cccgccgcca cgggctacaa gtacgtcatg ccccagccgt tcaggcagct
     1681 tttcagcaat ttctatcgaa atctgacggc cccggtccgc atcctgaaca atctttttca
     1741 gggcaagccg ggctatgccg gggaagagtt ggcgaagctc ctcatcaact ccacggtcgg
     1801 cgtgggagga ctgcggaact gcgccgagga gtgtttcggc ataaagggcc gcaacgccga
     1861 ttttggccag actttgggca agtggggcct gggccccggt ttctatctcg tcctgccttt
     1921 tctcggccca tcgagccccc gcgacgggtt cggttttctc gtggattggg ccctgaggcc
     1981 gcagacatac gttggtacgg agttcttcga ttaccagaac gtgggcctct tcgtgcatga
     2041 gaagatcaac agcacgtcgt tccgcatcgg tgaatacgag gcgctcaaga aggcggccgt
     2101 ggatccctac gtatccatgc gcgacatcta catccagtac cgcacggacc tcatcgagaa
     2161 ccgctagagg cccgggaggg atcaggcagc cctcttcagg agttccagga ccccgtcgag
     2221 aaccttaagg ctcttccgta ctttcccggg catcgcgaca acctcttcga aagaccccct
     2281 gatggtccgg ccggcctcta tcgcttcatc aacatcgttg ccgcgggaaa gggatgtcag
     2341 ttcatggaag aattcgaact cgcgcaccag ataggacagg accggccagg aagccgggtc
     2401 ttcgttcagt tccgcatgta ccgcctgaag cagggacccc tgatcctcat gaatgtcttc
     2461 ggcgatcttc tcgacaaggg ggatcagccg tgcttcgtac gggccggggg cctgccccga
     2521 tgcctgcttt gacctggcga catcttccac cttgaggaca acgatcccaa cgaagtcatg
     2581 gacaaaccgg ccccatgaac cggcaaatgt ctctgccagc gcgtctatct tcttcatggt
     2641 accctcccgg catctttgtt ctcgtaatac agtaattata tcccactctc cgcatcctgg
     2701 aaggggcgtc ctcgcgctcc tgtacttggc gtacatctgg ccgtataata ttctcaggtt
     2761 cttcatgaac cgggaaagaa aaaaaggggg gcggtcatgt cactattgtt catccagtac
     2821 tgggatgtca ttcagggcag ggagaaggag tacacggatt acctgaacaa cacgtatctt
     2881 gcagaggtta cgaccctggg tctcgtgccg gtggggggat acttcgtcga ggtgggtttc
     2941 gggccgagga cgataactgc gttctcgtcg gagagcctgg aggatatttc caggatcata
     3001 accggcaagg aattcaggga ccttgccctg ggactgcgaa agtatgtggc caacttcaac
     3061 aacaccgtgc tcgagcctga cggcacggtc aagctgggga agtatcccgt ccagaagggc
     3121 gtctggaagt tcaaccagta ctatgacctg aagcccgaca tgaaggaggg gtaccaggac
     3181 ttcatcgtca acgaatacct cccggcaatg gcgaagatcg actacgtcac ggtgacgaac
     3241 tgctggaacg tggtgctcgg cggcttttgc gacatcgtcc tcgagatcac cttcaaggac
     3301 cccgaggaca tcgggcacct gctcaagaac aacgacctcc agaggatcac ccgcagactg
     3361 atccgcgagt tcgtgacgaa ctacaccaac cggatacagc ggacgacaaa atggttcagt
     3421 gacccgagat ggttcagaag gtagggcctc gtacacggcc agggggtcat cgatacactt
     3481 cccgcctgtg gccgatgcga acaacgagga tcaggagttc tctctccttc ttctcgaaga
     3541 tgatccggta gtttccgaca cgcagcctgt agtattcttc gtccgtgccg gaaagcctgg
     3601 tgatgttgtt cttgaggacg tcggggttct cagccagaat gaggagtttt tcctttatga
     3661 tcctttggaa ggggaaatcg atccccttga ggtcctccac ggctttgcca aggaactgaa
     3721 gggtatacac gtctcagata ccgatctctt tccaaacgtc tctggcgtcg acggtcctgt
     3781 cccgcccttc cttgagatcc ttcattctct tttgcgcaac cctgaggtcg agaaggtcaa
     3841 agtagaccat cagggctttc tcgatgacgg agcttttctt ttcccccagt tctcccgcga
     3901 cggaatcgag ctcccgcgcg atatcttcgc tcagtgttat gttgtgccgt gtttgcatgg
     3961 tcacctctga atacacataa ttatacacat atttggtgta atgtcaagaa aggaggttct
     4021 cttcacgctg gcggcggttg ccgcctttta aaaggcacgg caaaatggta gagtgaggca
     4081 gcggacgtcc ggggcgctgc cctgcgcccg aggggcgcgg gtttatcgag aaggggtctc
     4141 gctgatgacg gagaagggat atgagatatg atttcgataa ggctgtcgag cgagaaggca
     4201 cggacagcat taaatggaga cgatacggcg gcggggtcct ccccttgtgg gtcgccgaca
     4261 tggacttcgt ttccccggaa ccggtcgtcc gggccctgca cgaacgggtg gaccaccggg
     4321 tcttcggata tggaggggcg acggaggagt tcgtggaggt catccgggag agactgaaaa
     4381 ggctctacgg atgggatgtc cagggggagg agatcgtcct tgtgcccggg gtggtaacgg
     4441 gcctcaacct ggcgttcaca ctcttcgcgg agccggggga ggccgttctc gtccagccgc
     4501 cggtctattc gcacttcata gccgacccgg tgaaccgggg acgcacggtc atcgatcctc
     4561 cgctggtaaa aaggggggac acctacgaga tcgatttcga cgccttcgaa gaggcgatca
     4621 cggacaggac gcggatcttt gtcctgtgca atccccacaa ccccgtcggc cgggtcttca
     4681 gaaaggagga gctcgaaagg cttgccgata tctgcctgag gcgggacgtc cttatctgct
     4741 ccgatgagat ccactgcgac cttctgttcc ccggcaaccg gcacttaccc atcgccacgc
     4801 tcggcaatga ggtggcggac cgcaccatca ccttcatgtc gccgagcaag acgttcaacc
     4861 tggcgggtct gaagtgctcc ttc
//
LOCUS       DAOQTR010000094         4804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_19640_length_4804_cov_3.434407, whole genome shotgun sequence.
ACCESSION   DAOQTR010000094 DAOQTR010000000
VERSION     DAOQTR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19640_length_4804_cov_3.434407"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKN85_03355"
     CDS             complement(<1..346)
                     /locus_tag="PKN85_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43470.1"
                     /translation="MRKSSAMMAVLLTGLLSCTVAHGGTVWDGAGGNTNINTAGNWDD
                     NVNPPFDGTVSVAFGTGGSTATINTNVSFLGLIFNRDSNFTVAAGAGTLTIGAGGIDA
                     AIPGTTSRTYTIT"
     gene            complement(767..1918)
                     /locus_tag="PKN85_03360"
     CDS             complement(767..1918)
                     /locus_tag="PKN85_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT43471.1"
                     /translation="MIERRSWIKRIEEVWEETPIVWLSGVKGVGKTTLIQGLGTQRTL
                     YVDCDPARSAHIAADPEFFFRACDRPVVVFDDVHKLPDAMKILKTGADLFPELRIIAT
                     SSTVAADGKAGDVLGDRKRRVHLGPVLWNELPAFNVSLERRFYHGGLPEALLTEHRQG
                     GFYREWMDAFFDRDIREPFGFRDSDKFTMLFKYLLKQSGEPFETTRASETLRISRPTV
                     AEHVRALDATGAITVVRPFHGKGQKEIIKMPRVYGFDTGFVAFARGWDPLRPDDYGPL
                     WEHIVLEFLETHVRGSQVQYWRWADDRKVDFIVPGTRDEVDTVECEWNPDAFDPAGLK
                     LFRTWYPEGKNYIISPLSSAAVERHIAGLDVHVCDLEGWLERQGASRKD"
     gene            2144..2563
                     /locus_tag="PKN85_03365"
     CDS             2144..2563
                     /locus_tag="PKN85_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNT43472.1"
                     /translation="MKIVNEHREFSTQGSGDLIDITRDVAGCLVGSGLTNGSMTIFVQ
                     GSTAGITTFEYEPGLIGDVQEFYERMVPSDRAYRHDETWGDANGFSHLRATLTGQSLT
                     VPFESGVLLLGSWQQVVLAEFDNRPRERLVVVQMIGE"
     gene            2661..3140
                     /locus_tag="PKN85_03370"
     CDS             2661..3140
                     /locus_tag="PKN85_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43473.1"
                     /translation="MCRLLPLKSIALFLGILLSFLSLGGCRKPEPARVILAVDGEMFS
                     DALVIIDGKQAGKLTQTLIKPDGNLYIDGILTAKLPPGHGDIREKDDYQGTIDSLELM
                     AGEHTILLQTEDGKTLQITARIPPGRHLVTYLSNEETLTWNSAKLKAPPGSSVNVRP"
     gene            complement(3312..3764)
                     /locus_tag="PKN85_03375"
     CDS             complement(3312..3764)
                     /locus_tag="PKN85_03375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43474.1"
                     /translation="MTRAKEDDTRRDAVKGPAERSFMPEFVGFVRGMALECCYSDKTV
                     DDLGQAIEEAVSNIIRFACREKPGEIEISCTVHDTGALYITIADTGAPFNMLLAGTFP
                     EADDFFGSGERPSTKIMKRAAKNIEYKRSSNRNILIFTVSHDPGGMRR"
     gene            complement(3795..>4804)
                     /gene="amrB"
                     /locus_tag="PKN85_03380"
     CDS             complement(3795..>4804)
                     /gene="amrB"
                     /locus_tag="PKN85_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT43475.1"
                     /translation="ASGEMVYIERIEELVRVMDENLLLEGERYDRHVKGLRDEYCNAP
                     VRKAYLAGKGYASNRMELLSFLDGLFDGNAVTPAPAREIAGILAPHIDYQRGATVYRE
                     IYPYLRGGAKPLIVIFGTCHRPTDGLWSISLKDFETPLETIPCGQGLRELIGANDLLK
                     RYVDEWPHRVEHSIELQLPLIQFMVEEDFEILPILTGSMHEYVGGLKETDDEEVKLLV
                     ENLKGVLSAYGKPCLILSGADLAHIGAQFGDRYPLDRTILESSRRKDEAMLEHIRNVD
                     AEGFFRAVKSEGDMRRICGLTPIFFQLRLLEGCTSDIVSYDQWTDGQSSVSFAGGVFY
                     R"
BASE COUNT          974 a         1429 c         1401 g         1000 t
ORIGIN      
        1 cggtgatggt gtaggtccgt gatgtggtgc cgggaatggc tgcatcgatg ccccccgccc
       61 cgatggtcag ggtcccggcg cctgccgcaa cggtaaaatt gctgtcacgg ttgaagataa
      121 ggccgagaaa actgacattg gtgtttatgg tggccgtgga cccgcctgtc ccgaatgcca
      181 cggacacggt gccgtcaaag ggagggttga cgttgtcatc ccagttgccg gccgtgttga
      241 tgttggtatt cccccccgcg ccgtcccaga cggtccctcc gtgagcgacc gtgcaggaca
      301 ggaggcctgt gagcagaacc gccatcatgg ccgagctttt tctcatatcg gctgcacagc
      361 ctctccgtta cccgggttgt cgcggttgat ggtctcaggt gtgcgaactc tgacaactcc
      421 ccgccgcacg ggcggggacc tctgctgttg taccattcag atacaacggg gaggaaactt
      481 cctcattggc ctcattgttc tacaatttta tatgtattac aagagaaatc atacaggcga
      541 agacactcgg ctgtgacgac cgccggagct cttctcgaag ggcctctgcc gcaccttcat
      601 cgatgaatcc aggaggagcc aacagctcgt cacgcagatc gagaaggcag cctcccgcct
      661 cacattcctg aaccacgtca taaggggaaa ggaccaggag gaattctgcc tctttttcgg
      721 gaggctgcga gggagcatct cgttagcgaa atacgttttc ccccggctag tctttgcggc
      781 tggcgccctg cctttcgagc catccctcaa ggtcgcagac gtgaacgtcg agtccggcga
      841 tgtgtctttc aacggccgcg ctcgacaggg gagagatgat gtagttcttt ccttcgggat
      901 accaggtgcg gaagagcttg agccccgcgg ggtcgaaggc gtcggggttc cactcgcatt
      961 cgacggtatc cacctcatcc cgcgtgccgg ggacgatgaa atccaccttt cggtcgtccg
     1021 cccatctcca gtactggacc tgggacccac ggacgtgggt ttcaagaaac tcgaggacga
     1081 tatgctccca gaggggaccg tagtcgtcgg gacgcagcgg gtcccaaccc ctggcaaagg
     1141 ccacgaaacc cgtgtcgaac ccgtagaccc tgggcatctt tattatctcc ttctgccctt
     1201 ttccgtggaa aggccggacc acggttatcg ccccggtggc atcgagggcc cttacgtgct
     1261 ccgcgacggt gggacggctg atgcggaggg tctccgatgc ccgtgtcgtc tcgaagggct
     1321 cgccgctctg tttcagcaga tatttgaaga gcatggtaaa cttgtcggaa tcacgaaacc
     1381 cgaagggctc gcggatatcg cggtcaaaaa aggcgtccat ccattcacga tagaacccgc
     1441 cctgccggtg ttccgtcaaa agggcctcgg ggagcccgcc atgatagaat cgcctctcca
     1501 gagaaacgtt gaaagcggga agctcgttcc acagaacggg gccaaggtgg acgcggcgct
     1561 tcctgtcacc gagtacgtca ccggccttcc cgtcggccgc cacggtggac gaggttgcaa
     1621 tgatcctgag ctccggaaaa agatcggctc ccgtcttgag gatcttcatg gcgtccggga
     1681 gcttgtggac atcgtcaaag accacgaccg ggcggtcaca ggcgcggaag aagaactcgg
     1741 ggtccgctgc tatgtgcgcg gaccgggccg ggtcgcagtc gacgtagagc gtccgctgcg
     1801 tcccgagacc ctgtatcagg gtcgtcttgc ccaccccttt cacgccggaa agccacacaa
     1861 tgggcgtctc ctcccagacc tcttctattc tctttatcca ggatctccgt tctatcattg
     1921 agacaacaca gttaccatat attcggaata aacgtcaagt agagtgtacg aaagacaggg
     1981 cgcccgatgc ggcccttgct ctcaaaaccc ttccaatgcg cccctctctc tggtagggta
     2041 atgaaggcag tcatgggcaa tggagttata ggtgatgggg ggaaggtccg gtctttccca
     2101 tcacccatga tctattacct gtattctcac gagaggtgat ttcatgaaga tagtgaacga
     2161 acacagggaa ttttcaaccc agggaagcgg agaccttatc gacatcacca gggacgtggc
     2221 cggctgcctc gtggggagcg gactgacgaa cggcagcatg acgatcttcg tgcagggctc
     2281 cacggcgggc atcaccacct tcgagtatga accgggcctt atcggcgacg tgcaggaatt
     2341 ctacgaaagg atggtccctt ctgatcgcgc gtaccgccac gacgagacat ggggtgacgc
     2401 gaacggattc tcccacctca gagccacgct gacaggccag tccctgacgg tgcctttcga
     2461 atcgggcgtt ctgcttctcg gcagctggca gcaggtggtc ctcgccgaat tcgacaaccg
     2521 cccgcgggaa cggctggtgg tggtgcagat gataggggaa tgaaacggtt gcaacccatt
     2581 cttttctgga ctatactgag tcgtatttca ccgtgcgtac gctgtaataa ggaaggatct
     2641 tcgagaatcg gggaaatctc atgtgccgtc tgttgccgct gaagtccatc gccctcttcc
     2701 ttggaattct cctgtcgttc ctttccctgg gaggttgcag gaaacccgag cctgcccggg
     2761 tcatccttgc cgtcgatgga gagatgttct cggacgccct tgttatcatc gacgggaagc
     2821 aggccgggaa gctcacgcaa acactcatca agcccgatgg aaacctgtac attgacggca
     2881 tcctcaccgc aaagctgccg cccggccacg gggacatccg ggagaaggac gattaccagg
     2941 gcacgatcga ttccctggag ctcatggccg gcgagcacac catcctcctc caaacggaag
     3001 acgggaagac gctgcagatc acggccagga taccccccgg ccgccacctc gtaacctacc
     3061 tctccaacga ggaaaccctc acgtggaaca gcgcaaaact caaggccccc ccggggtcaa
     3121 gcgtgaacgt aagaccgtaa agacggctgc aaccgcttga accgcttgaa cgttgaagag
     3181 ctcagaaatg agaaggcttg tgaggatacc gggcttgcgg cggaacggag acggggcgat
     3241 cacggccaat ggtcaggaaa ggtccttttg tctctgaacg ttcaagcggt tcaaactgtt
     3301 caagccgcct tttaccgcct catccctccc gggtcgtggg ataccgtgaa gatgaggatg
     3361 ttcctgttgg agcttcgctt gtactcgatg ttcttggcgg cccttttcat gatctttgtc
     3421 gacggtctct cgccggaccc aaaaaagtcg tcagcctcgg gaaaggtgcc ggcgagcagc
     3481 atgttgaagg gggccccggt gtcggcgatg gtgatataca gagcacccgt gtcgtggacg
     3541 gtgcaggaga tctcgatctc cccgggtttc tctcggcagg caaaacgaat gatattgctg
     3601 acggcttcct cgatggcctg accgagatcg tccacggtct tatcgctata gcagcactcc
     3661 agggccatac cccggacaaa acccacgaat tccggcatga acgacctttc ggccggtcct
     3721 ttgaccgcgt cgcgccgcgt atcgtcctct ttcgctctcg tcattctcac ctcgatcgtt
     3781 cctgacggta ttgttcaccg gtagaagaca ccgccggcaa agctgaccga cgactgtccg
     3841 tccgtccact gatcgtagct cacgatgtcg gacgtgcacc cttcgagaag tctgagctgg
     3901 aagaaaatgg gggtgagccc gcatatcctc ctcatgtccc cctcgctctt gacggcacgg
     3961 aaaaaaccct ccgcgtcgac gttcctgata tgctcgagca tggcctcgtc tttgcgccgc
     4021 gagctctcga ggatcgtacg gtcgagggga taacggtcgc cgaactgggc cccgatatgg
     4081 gcgaggtcgg cgcccgaaag gatcaggcag ggcttcccgt aggcggaaag cacgcctttc
     4141 aggttctcga cgagaagctt tacctcctca tcgtccgtct ccttcaaacc gcccacgtat
     4201 tcgtgcatgg agcctgtaag gatgggcagg atctcgaagt cctcctcgac catgaactgg
     4261 atgaggggga gctgcagttc gatggaatgc tccacccggt gcggccactc atcgacatac
     4321 cttttcagga gatcgtttgc gccgatcagc tccctcaaac cctgtccaca gggtattgtc
     4381 tcgaggggcg tctcgaagtc cttgagggat atgctccaga gtccgtccgt gggacgatgg
     4441 cacgtgccga agatcacgat gaggggcttc gcgcctcctc tcaggtatgg gtatatctcg
     4501 cggtataccg tggcgcccct ctggtagtct atgtgcggcg caaggatgcc cgcgatctcc
     4561 cgtgccgggg caggtgtcac cgcgtttccg tcaaaaaggc cgtcgaggaa ggacagaagc
     4621 tccatccggt tcgacgcgta tcccttcccc gcgaggtacg ccttcctgac gggggcattg
     4681 cagtactcgt cgcgaaggcc ctttacgtgc ctgtcgtagc gctccccttc gaggaggagg
     4741 ttctcgtcca tgaccctcac gagttcctcg atgcgctcga tgtagaccat ctcaccggag
     4801 gccc
//
LOCUS       DAOQTR010000095         4735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20034_length_4735_cov_4.145513, whole genome shotgun sequence.
ACCESSION   DAOQTR010000095 DAOQTR010000000
VERSION     DAOQTR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20034_length_4735_cov_4.145513"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..618
                     /locus_tag="PKN85_03385"
     CDS             70..618
                     /locus_tag="PKN85_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNT43476.1"
                     /translation="MSYPAVPFDRCYWVVPGRFLAGCYPGDLDAGVACDKVQGLLDAG
                     IRHVVNLMEETETNWDGEPFAGYREEFVRRAGQEGVDVGWVRRPIRDLAVPPREHMAG
                     ILDEIDGAIADGRPVYVHCWGGKGRTGTVVGCYLARHAFASGERVLDMIRELRANVPG
                     GGYDSPETGEQRDMVSSWQIGE"
     gene            600..1592
                     /locus_tag="PKN85_03390"
     CDS             600..1592
                     /locus_tag="PKN85_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNT43477.1"
                     /translation="MADRRVKMTGISRRDRMLGGLWGAVVGDALGVPVEFRGRDIRKG
                     DPVTDMRGYGTFNLPPGSWSDDSSLMLCTAQSLLEGFDLGRMGDLFVSWFTAGLWTPH
                     GQAFDVGRGTWQAISRMQLGTPAATAGGREEENNGNGSLMRILPVALYCAAAGDDELL
                     RIAHEASAVTHGHPRSMMACGVYCFLIAALLDGMPPREAYLLAMEKMKRLYGGPPFSE
                     ELAHFTRLLSGDIETVPEAAIESDGYVVHTLEASLWCLLGTDAYNSAVLRAVNLGWDT
                     DTTAIVTGGLAGTYYGLEAVPLQWRDTLARKQEIEALFERFVEKMICPFAFSSG"
     gene            1609..1857
                     /locus_tag="PKN85_03395"
     CDS             1609..1857
                     /locus_tag="PKN85_03395"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HNT43478.1"
                     /translation="MADLIGYIAACFTTFSLLPQILRIRRLKEARDVSLFLPLMIVIG
                     SVLWSVYGILISSIPVIVANGVALIIALITVFFTIRYR"
     gene            complement(1868..2314)
                     /locus_tag="PKN85_03400"
     CDS             complement(1868..2314)
                     /locus_tag="PKN85_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43479.1"
                     /translation="MKIEFTKKQFETLLKAVYLGARTANAYEDDLEGNEYVELQKYIF
                     GFARDFGIEKYADYDEKLKVAWPSATLEEDEVLNELIDRYDDFTFLDKLIYNLAGRDM
                     LDKHGPKALEKMSDDEFFKEEEVFAEKYRKEFAKNGIKNLSISKGK"
     gene            complement(2363..3403)
                     /locus_tag="PKN85_03405"
     CDS             complement(2363..3403)
                     /locus_tag="PKN85_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT43480.1"
                     /translation="MKKRPVSLVCGFIVIIVVFVSGAIAGEKESSEEALKKFFDDQKT
                     RTGTITDNAAAMKDYVSSVRDVNARYKNGNTLLHYAANRGYLEVTALLLKKGADINAR
                     DKDGRTPLHEAMAYRRYDVARFLVENGADMTVKNKDGETPLISIVYMDNKKTAVDLVN
                     FFIAKGFNVSRSADAKLLNESIGRGHHDVAVILLEKGIAFDDSSLRGAASMGYEDIFV
                     TLLSRGADPRHKDILRAASASGNVGILKTLLEKGQRPTAEDVDLALYKGRRDAAVLLN
                     EALKRSGGQDVDLTARCRMKADPGPCMALFDAGYYDEIAGRCRSFTYGGCGGSAPFET
                     ESACKRICEDNK"
     gene            3601..>3944
                     /locus_tag="PKN85_03410"
     CDS             3601..>3944
                     /locus_tag="PKN85_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT43481.1"
                     /translation="MTRALVYACIIFSVVFSFSNAVFAAGAYPEKLTAVYTDWYPYTY
                     NEGNSAKGFEIEVFTAVVNRMGIEVVFERFPFKRCLKMLEEGRAHVLISLLKTPERRQ
                     YTVFPDEHLSVSR"
     assembly_gap    3945..3954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3955..4420
                     /locus_tag="PKN85_03415"
     CDS             <3955..4420
                     /locus_tag="PKN85_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT43482.1"
                     /translation="SRTSLFTRTDTRVRYRGSLADLKGYTIGVVAGFSYGTLFDNADY
                     LKKEEVQNTRRLISMVVSGRLPLGIENEAVIRGVAGSLGVAGSIRFLNPPVHSRKLYA
                     GFSRRLELRKLAEEFSRSLKDFKNSGEYRDILRKYDVSHSELTRQREKTSGS"
     gene            4517..>4735
                     /locus_tag="PKN85_03420"
     CDS             4517..>4735
                     /locus_tag="PKN85_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43483.1"
                     /translation="MDIFRRRILAKLIIILIVAVSAISVASILYFDRRNRGTLERELE
                     DAVSNLGEIGRLAYAVPMWQMAKTEIAYI"
BASE COUNT          921 a         1279 c         1422 g         1103 t
ORIGIN      
        1 gaaagtcgcg gcgtgagaaa tatattggtg tctactattg acatcggagg tcagaataag
       61 aggagaagtt tgtcctatcc cgcggttccc tttgacaggt gttactgggt ggtgccgggg
      121 aggtttctgg cggggtgtta cccgggagac ctcgatgcgg gtgtggcgtg cgacaaggtg
      181 cagggactcc ttgatgccgg catacggcac gtcgtgaacc tcatggaaga gaccgagacg
      241 aattgggacg gagaaccttt cgccgggtac cgggaggagt ttgtccgacg ggcggggcaa
      301 gagggcgtcg atgttggctg ggtgcgcagg cccatcaggg accttgcggt cccgccccgg
      361 gagcatatgg ccggcatcct cgatgagatc gatggcgcca tcgccgacgg caggccggtg
      421 tatgtccatt gctggggcgg gaaggggcgg acggggaccg ttgtcggctg ctacctggcg
      481 cgacatgcct tcgccagcgg ggagagggtg ctcgatatga tacgggagct aagggcaaat
      541 gtacccggtg ggggctatga ttcgcccgag acaggtgaac agagggacat ggtgtcctcg
      601 tggcagatag gagagtgaag atgacgggga tctccaggcg ggacagaatg ctcggcgggc
      661 tctggggggc cgtggtcgga gacgccctcg gtgttccggt ggagttcagg ggaagggata
      721 tccgcaaggg ggatcccgtc acggacatgc ggggatatgg gaccttcaat ttgccgccgg
      781 gaagctggtc ggatgactcg tcactgatgc tctgcacggc gcagagcctt cttgaggggt
      841 tcgacctcgg gcgcatgggc gatcttttcg tcagctggtt caccgcgggc ctctggaccc
      901 ctcacggaca ggccttcgac gtggggaggg ggacgtggca ggcgatcagc aggatgcagc
      961 tcggcacacc ggctgcgacg gcgggtggac gggaggaaga gaacaacggc aatggctcgc
     1021 tgatgcgcat ccttcccgtg gccctctact gcgcggcagc gggagacgat gaactgctcc
     1081 gcatcgctca cgaggcttcg gccgtaacgc acgggcatcc cagatcgatg atggcctgcg
     1141 gagtgtattg cttcctcatc gcggccctcc ttgacgggat gccgccacgc gaggcatatt
     1201 tgctggctat ggaaaaaatg aaaaggttgt atggcgggcc tcctttttcg gaagagttgg
     1261 cccattttac ccgtcttctc tccggtgata tcgagaccgt tcccgaagcc gccatcgagt
     1321 cggacggcta cgtggtccac acgctggagg cgtccctgtg gtgtctgctc gggacagatg
     1381 cctacaactc ggcggtgctc agggcagtga atctcggctg ggacaccgac acgacggcca
     1441 tcgtaaccgg cgggctcgcc ggcacctatt acggccttga agcggtcccg ctgcagtgga
     1501 gagacacatt ggcgaggaag caggaaatag aagccttgtt cgaacggttt gtggagaaga
     1561 tgatctgccc ttttgctttt tcgtccgggt gatgataaga agatagaaat ggctgacctc
     1621 atcggttata tagccgcttg tttcacgacc ttttcgcttt tgccgcagat actgcgtatc
     1681 cggagactga aagaggccag ggacgtctcg ctttttctgc ccctgatgat cgtcatcggt
     1741 tccgtgctgt ggtcggttta cggcattctc atcagctcga ttcccgtcat cgtggcgaac
     1801 ggtgtcgcac tgatcatagc tctgatcacc gttttcttca cgatccgata cagataaggc
     1861 gagcctctca tttccccttg gatatcgaga ggttctttat cccgttcttt gcgaattctt
     1921 tccggtactt ttcggcgaag acctcttctt ccttgaagaa ttcgtcgtcg gacatctttt
     1981 ccagcgcctt gggtccatgt ttgtcgagca tgtcccgtcc cgcgagattg tagatgagct
     2041 tgtccaggaa cgtgaaatcg tcgtagcggt cgataagctc gttcaggacc tcatcctctt
     2101 caagggtggc cgagggccag gccactttca gcttttcgtc gtagtcggcg tatttctcga
     2161 tcccgaaatc cctggcgaac ccgaaaatgt atttctggag ttcaacgtac tcatttccct
     2221 caagatcgtc ttcgtaggcg ttggccgtcc ttgcccccag gtatacggcc ttcagcagcg
     2281 tttcaaactg tttcttggta aattcgatct tcatattctc cctcttgtcg ggtctgggtc
     2341 agtatccgtc ttccatgacc cgttacttgt tatcctcgca tattctcttg cacgcgcttt
     2401 ctgtttcgaa gggcgcggat ccgccgcatc ctccgtaggt gaaggatctg cacctccctg
     2461 ctatctcgtc atagtatccc gcgtcgaaaa gagccatgca cggtccgggg tccgctttca
     2521 tccggcatct tgccgtgagg tctacgtcct gaccacccga tcttttcagt gcctcgttga
     2581 gaaggacggc ggcatcccgc cttcccttgt agagcgcgag gtcgacatcc tccgcggttg
     2641 gtctctggcc tttttccagc agagtcttca agatgccgac gttcccggag gcagaggcgg
     2701 cacgaaggat atccttgtgc cgcgggtccg caccccgtga gagaagggtg acaaagatgt
     2761 cctcatatcc cattgacgcc gcgccacgaa gcgaggagtc atcgaaggct ataccttttt
     2821 ccaggaggat gacggcgacg tcatgatggc cccttccaat ggattcattg aggagctttg
     2881 catcggcgga gcggctgacg ttgaatcctt tcgctataaa gaagttgacg aggtcgacgg
     2941 ccgttttctt gttatccatg tagacgatac tgatgagcgg cgtctcgccg tccttgttct
     3001 tcacggtcat atccgcgcca ttctcgacaa ggaaccgggc gacatcgtac cggcgatagg
     3061 ccatcgcttc gtgcaggggc gtccttccat ccttgtcccg ggcattgatg tcggcccctt
     3121 ttttcagcag cagcgccgtc acctccaggt accccctgtt ggccgcgtaa tggagcaatg
     3181 tgtttccatt cttatagcgg gcattgacgt ccctgacgga agaaacgtag tccttcatcg
     3241 cggcggcatt gtcggtgatc gtcccggtcc gagtcttctg gtcgtcgaag aatttcttaa
     3301 gggcttcttc cgaggattcc ttttccccgg cgattgcgcc ggagacaaac acgacgatga
     3361 tgacgatgaa tccacatacg agagatacag gtcttttctt cattctggtt gtcactcctt
     3421 catttggtgc gcctgccact gtcccccacg gacccccgcg cgtttttttc atcacagatt
     3481 gtaatatcag gcgaggcaaa atggcaagcg tatcgagaaa ggagttgctc cggggtgacc
     3541 tcaacgtctt gacgacgggc gcggggtggc aatcccggcg gtaatatggt agaagttttc
     3601 atgacaaggg cattggtcta cgcgtgcatt atcttctccg tggtcttttc cttcagcaac
     3661 gcggttttcg ccgcgggtgc ctaccctgag aaattgaccg ctgtctacac ggattggtac
     3721 ccgtacacct ataacgaggg caacagcgcc aaggggttcg agatagaggt cttcacggcc
     3781 gtcgtcaaca ggatgggcat cgaggtcgtc tttgagcggt tccctttcaa gagatgtctc
     3841 aagatgctcg aggagggtcg tgcccatgtc ctcatttcgc tcctcaagac cccggaacgc
     3901 aggcaatata cggtattccc cgatgagcat ctcagcgtct cccgnnnnnn nnnnctcccg
     3961 gacctctctc ttcaccagga cggacacgcg ggtccggtac aggggttccc tggcggacct
     4021 gaagggatac accattggtg tcgtcgcggg cttcagctac ggcacgctct tcgacaatgc
     4081 cgattacctg aagaaggagg aagtgcagaa caccagacgg ctcatcagca tggttgtcag
     4141 cggcaggttg cctctcggca tcgagaacga agcggtcatc aggggtgtcg caggcagcct
     4201 tggcgtcgcg ggctcgatcc gttttctcaa cccccctgtc catagccgga agctctatgc
     4261 gggcttctcc aggaggctcg agctccggaa actggctgag gaattttccc gaagcctcaa
     4321 agacttcaag aattccggcg aatacaggga catactgcgg aaatatgatg tttcccattc
     4381 cgaattgacc cggcagaggg agaagacttc cggcagttga cttttcttac gattcgccga
     4441 aaataataga agagggtcag tctgaccggt tcctgcacgg gaagtctttc ggggctggta
     4501 gagagaggaa acattcgtgg acatattcag gaggaggata cttgccaaac tcataatcat
     4561 attgatcgtt gcggtatccg cgatcagcgt cgcatccatc ctctatttcg acagaaggaa
     4621 cagggggacg ctggagagag agctagagga tgcggtgtcg aacctgggag agatcggccg
     4681 actcgcctac gctgtcccca tgtggcagat ggcgaagacg gagatcgcct acatc
//
LOCUS       DAOQTR010000096         4719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20133_length_4719_cov_3.819897, whole genome shotgun sequence.
ACCESSION   DAOQTR010000096 DAOQTR010000000
VERSION     DAOQTR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20133_length_4719_cov_3.819897"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKN85_03425"
     CDS             <1..729
                     /locus_tag="PKN85_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNT43484.1"
                     /translation="KLTLMMPETMSIERRKVLKALGANIILTEGAKGMKGAIAKAEEM
                     VRSDASRYFLPQQFENPANPAIHEATTAVEIWNDTEGKVDILVCGVGTGGTITGISRY
                     FKLTKKKKVTSVAVEPIHSPVLTQTRAGEELKPGPHKIQGIGAGFVPKVLDLSLVDRI
                     ETVSNDEAIEMARRLAREEGILSGISCGAAAHAAVKLAGERENAGKTIVVILPDAGER
                     YLSTVLFEGILGGVESAGASDTPI"
     gene            792..1598
                     /gene="larE"
                     /locus_tag="PKN85_03430"
     CDS             792..1598
                     /gene="larE"
                     /locus_tag="PKN85_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNT43485.1"
                     /translation="MKERLERKYDDLRRSIGAMGKVAVTFSGGVDSTLLLKVCVDVLG
                     SANVLALTGVSPIHPRDEREEARALAAELGVRMVAFDSAEMEDESFLANGLERCYHCK
                     THLFKSVREIARQEGYSLVLEGSNVDDMNDFRPGRKACVEQGVKSPLLEADLNKEEVR
                     TLSRELGLKTHDKPAEACLSTRIPYGTPITVGRLRDIERAEAFIKALGISRVRVRHHG
                     SAARIEVEEKDLPLIIEQRNPIAGELTDIGFTYVALDLRGYRTGSMNEGQ"
     gene            complement(1792..2745)
                     /locus_tag="PKN85_03435"
     CDS             complement(1792..2745)
                     /locus_tag="PKN85_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43486.1"
                     /translation="MQERENSRDLCREEIDATQKHRSRLPLLIDEVVAQYDKEGRLHH
                     IGPEPIPSRQAIINIIRKTIPILFPGFFAEKRLDEINARYYLGEQITELFDAISTQIQ
                     HAIRHDCIRFDRPCVHCEDLSQEITLRFIGSIPRLQETLSKDVRAAFQGDPASRHFDE
                     IIFCYPGLFAVAVYRVAHWLHVNEVPLIPRIMTEYAHSLTGIDIHPGVVIGESFFMDH
                     GTGVVIGETTTIGDRVRLYQGVTLGALSLGKDECPLLRDKKRHPTIEDDVIIYANATI
                     LGGRTVIGARSTIGGNVWLTESVPPDTSVFLKKPELVMKNK"
     gene            complement(2758..3939)
                     /gene="nifS"
                     /locus_tag="PKN85_03440"
     CDS             complement(2758..3939)
                     /gene="nifS"
                     /locus_tag="PKN85_03440"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNT43487.1"
                     /translation="MRTVYLDNNATTMVSREVLDEMLPFFHDRYGNPSSMHSFGGRVA
                     RELSDARHKVASLLGADPEEIIFTSCGTESDNTAIMSSLLVKPGKTHIVTSRVEHPAI
                     KVLCEHLAQKGYRVTMVGVDSDGRLDMDEYEASLTPDTAIASIMWANNETGVIFPVEE
                     AARLARDRGIVFHTDAVQIVGKIPIDMKGNAVDMLSLSGHKLHAPKGIGVLYVRKGTR
                     FSPFLIGGHQEKGRRGGTENVAGIVGLGKACELAAASMEKENTYVRALRDRLENSLLA
                     AIPQSRVNGVRDERLPNTTNISFEFVEGESILLLMDQHGICASSGSACTSGSLQPSHV
                     LRAMGVPYTMAHGSIRFSLSVYNTEEDIDLVIRELPPIIDKLRVLSPYWATERGACTT
                     V"
     gene            complement(3936..>4719)
                     /gene="nifU"
                     /locus_tag="PKN85_03445"
     CDS             complement(3936..>4719)
                     /gene="nifU"
                     /locus_tag="PKN85_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNT43488.1"
                     /translation="GEIEDPDGVAEVGSIACGDALKLSFKLDENKRIADAKFKTFGCA
                     SAIASASALTEIIKGKTIEEAMKITNQDIADYLGGLPQEKMHCSVLGQEALEKAIDNY
                     RGVSHKKPEEEIVCACFGVTDGEIERAIRQNHLTSVEEVTYYTKAGGGCGNCHDKIQA
                     ILDRVLAAKRSEKEPEERPRLSNIQKIRMIEETIEREIRPSLQHDGGDIDLIDVIGNR
                     VLVATRGACATCQASQITLKSFVEWKLKEFVSPDLVVEEVAK"
BASE COUNT          953 a         1334 c         1435 g          997 t
ORIGIN      
        1 aaactgaccc tgatgatgcc ggaaacgatg tcgatagaga ggcggaaagt cctcaaggcc
       61 ctcggggcga acatcattct caccgagggg gcgaagggca tgaaaggggc catcgcaaag
      121 gcggaggaga tggtccgaag cgacgcgtca cggtatttcc ttccccagca gttcgaaaac
      181 ccggcgaatc ccgccataca tgaggccacg accgccgtgg agatctggaa cgacacggaa
      241 gggaaagtgg atatcctcgt ctgtggtgtc ggcacaggcg gtacgatcac cggcatatcg
      301 agatatttca agctcacgaa aaagaagaag gtcacctccg tggcggtgga gcccatccat
      361 tcccctgtcc ttacccagac aagggccggc gaagagttga agcccggccc ccacaagata
      421 caggggatag gtgccggctt cgttccgaag gtcctggacc tttcccttgt cgaccggatc
      481 gagacggtca gcaacgatga ggcgatcgag atggcaaggc gtcttgcaag ggaagagggg
      541 atcctgagcg gcatatcctg cggagcggcc gctcatgcgg cggtgaagct tgccggggaa
      601 cgggagaacg cggggaagac catcgttgtg atactgcccg acgccggcga acggtatctt
      661 tccacggtgc tttttgaagg gattctcggc ggggtggaat ccgcgggtgc atccgacacg
      721 ccgatctgac ggtgcagcgt aacttgttcg ggcaaggagt acggtgccat gaaagaggtc
      781 atgggacgca gatgaaggag agactggagc gcaagtacga cgatctgaga agatcaatcg
      841 gagccatggg caaggtcgct gtgaccttct ctggcggggt ggacagcacc ttgcttctca
      901 aggtctgcgt cgacgtcctg ggaagcgcca acgtgctggc cctgaccgga gtgtcgccca
      961 tacacccccg tgacgaaaga gaggaggcac gggccctggc ggccgaactc ggggtccgca
     1021 tggtcgcgtt cgattccgcc gagatggagg acgagagctt cctcgccaac gggctcgagc
     1081 ggtgttacca ctgcaagacc catctgttca aaagcgtccg cgaaattgcc cgacaggagg
     1141 ggtattccct cgttctggag ggttcgaacg tcgatgacat gaacgacttc cggccgggca
     1201 gaaaagcatg cgtggaacag ggcgtcaaaa gtcctcttct cgaagcggac ctgaacaagg
     1261 aagaggtcag gaccctttca cgcgagttgg ggttgaagac ccatgataag ccggcggaag
     1321 cctgtctgtc gacaaggatc ccttacggta cccccataac cgtgggcaga ctccgggata
     1381 tcgagcgcgc cgaagctttc ataaaggccc tcggtatatc gcgggtccgt gtgaggcatc
     1441 acggcagcgc cgcccgcatc gaggtggagg aaaaggacct ccccctcatt atcgaacaga
     1501 ggaaccccat agcaggggag ttgacggaca taggattcac gtacgttgcc ctggatcttc
     1561 gcggctaccg aacgggaagc atgaacgaag gacagtagag gcggttccag gtttcaggtt
     1621 aaagacgaag acggacggtt tccggtagga ccggaacctt tacagtcatc tcaggccggc
     1681 tcaaacctcc ggacggtccg atgagatagg aaccttcaca gtagagaaac ctccttatgg
     1741 tcttccgacc tgagatacct aaaacctaaa acctaaaact taaaacggtc ttcacttgtt
     1801 cttcatcacc agttccggtt tcttcaggaa gaccgacgtg tcgggaggga cggattcggt
     1861 gagccatacg ttcccgccga tggtcgagcg ggcgcctatg acagtccggc cgcccaggat
     1921 ggtcgcgttg gcatagatga tgacgtcatc ctctattgtg ggatggcgtt tcttgtccct
     1981 gagaagagga cattcgtctt tgccgagtga aagcgcgccg agggttacgc cctggtagag
     2041 gcgcaccctg tcgccgatgg tggtcgtttc cccgatgacg acgccggtac cgtggtccat
     2101 gaaaaagctc tcaccgatga caaccccggg gtgtatgtcg atgcccgtca gactgtgggc
     2161 atattccgtc atgatcctcg ggatgagggg cacttcgttg acgtgcagcc agtgcgcgac
     2221 gcggtagacg gcaaccgcga agaggccggg atagcagaat atgatctcgt cgaaatgccg
     2281 cgaggccggg tcgccttgaa aggcggcgcg aacatccttc gagagcgtct cctgcagccg
     2341 gggaatgctc ccgatgaacc tcagggttat ctcctgactg agatcctcgc agtggacaca
     2401 gggccggtcg aacctgatgc agtcatggcg tatggcatgc tgtatctgtg tcgagatcgc
     2461 gtcgaagagt tccgttatct gttcccccag gtaataccgg gcgttgatct cgtccagccg
     2521 tttttccgcg aagaaaccgg gaaagagaat ggggatggtc ttgcgtatga tgttgatgat
     2581 agcctgtcgt gaaggtatcg gttcgggacc gatgtgatga agacggcctt ccttgtcgta
     2641 ctgtgccacc acctcgtcga tgagaagcgg cagccgcgag cgatgcttct gggtcgcgtc
     2701 tatctcttcc cggcataggt ctcgtgaatt ctccctttct tgcatatctc tctctcctca
     2761 gaccgtcgta caggcgcctc gttccgtcgc ccagtaaggt gaaaggaccc ggagtttatc
     2821 gatgatcggc ggcagttctc taatgacaag gtcgatgtcc tcttcggtgt tgtagacgct
     2881 gaggctgaac cgtatcgatc cgtgggccat tgtgtaggga acacccatgg cccgcaggac
     2941 atgggaaggc tgcagcgagc cggatgtgca ggccgagccc gaggaagcgc agatgccgtg
     3001 ctggtccatg agcaggagga tgctttcacc ctcgacgaac tcgaagctga tgttcgtcgt
     3061 gttgggaaga cgctcatcgc ggacgccgtt cacccggctt tgcgggatcg cggcaagaag
     3121 gctgttctcc agcctgtcgc gaagggccct cacgtacgtg ttctccttct ccatgctggc
     3181 ggccgcgagc tcgcaggcct tcccgaggcc gacgatgccg gcgacgttct ccgtaccgcc
     3241 ccggcgccct ttttcctggt ggccgccgat gagaaaggga gagaaccttg tcccttttcg
     3301 tacgtaaagg acgccgatgc cttttggcgc gtgcagctta tgacccgaaa gggagagcat
     3361 atcgaccgcg ttgcccttca tgtcgatggg tatcttgccc acgatctgga ccgcgtcggt
     3421 atgaaagacg atcccccggt cgcgggccag acgcgccgcc tcttccaccg gaaaaatgac
     3481 gcccgtttcg ttattggccc acatgatgct cgcaatcgcc gtgtccgggg tcagacttgc
     3541 ctcatattcg tccatatcga gcctgccatc gctgtccaca cccaccatcg tcacgcggta
     3601 ccctttctgg gcaaggtgct cgcacaggac cttgatcgcc gggtgttcga ccctgcttgt
     3661 gacgatgtgg gtcttccccg gtttcacgag aagggaggac atgatcgctg tgttatcgct
     3721 ttcagtgccg cagctcgtga agatgatctc ctcggggtcg gccccgagga gcgaggcaac
     3781 cttgtggcgc gcatcggaaa gttcgcgggc cacccggccg ccgaaggagt gcatgctcga
     3841 ggggttcccg taccgatcat ggaaaaaagg gagcatttcg tcaaggacct cccgggaaac
     3901 catcgttgtc gcgttgttgt caaggtagac cgtcctcatt ttgccacctc ctcaacaacg
     3961 aggtcgggcg agacgaactc tttcagtttc cactcgacga aactcttcag agttatctga
     4021 gatgcctggc acgttgcgca ggcccccctc gttgccacga ggaccctgtt cccgatgacg
     4081 tcgatcaggt ctatatcacc gccgtcatgc tgcagcgaag ggcggatctc gcgttcgatc
     4141 gtttcttcga tcatgcggat cttctggatg ttggaaagcc ttggtctttc ctccggttct
     4201 ttttcggatc tcttggccgc gagaacgcgg tcgagtatgg cctggatctt gtcatgacag
     4261 ttcccgcacc cgccccctgc cttcgtgtag tacgtcacct cctcgacgga cgtgaggtgg
     4321 ttttgcctga tcgcccgctc gatctcgccg tcggtgacac cgaaacaggc gcagacgatc
     4381 tcttcttcgg gtttcttgtg cgatacgccg cggtaattat cgatcgcttt ttcgagggcc
     4441 tcctgaccga gcacggagca gtgcatcttt tcctggggta gaccgcccag gtagtcggcg
     4501 atgtcctggt tggttatctt catcgcttcc tcgatggtct ttcccttgat tatctccgtc
     4561 agagccgacg cggaggcgat ggcgctcgcg cagccgaagg tcttgaactt cgcgtcggcg
     4621 atccgtttgt tctcatcgag tttgaaactg agtttaagag cgtcgccgca cgctatggaa
     4681 cccacttcgg cgacaccatc cggatcctcg atctcaccc
//
LOCUS       DAOQTR010000097         4715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20159_length_4715_cov_2.883262, whole genome shotgun sequence.
ACCESSION   DAOQTR010000097 DAOQTR010000000
VERSION     DAOQTR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20159_length_4715_cov_2.883262"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..832)
                     /locus_tag="PKN85_03450"
     CDS             complement(149..832)
                     /locus_tag="PKN85_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT43489.1"
                     /translation="MSDRIIEVSALKRSFQKDDIVIEVLKGVDFTADKGEFITIMGPS
                     GAGKSTFLHIIGTLDKPTEGTVLFGGQDIMSFSEDEQSRFRNEKVGFIFQFYHLLQDL
                     NVVENVMMPLLIRRMKPAEAEAKARNFLETVELTHRLNHKPGELSGGEQQRVAIARAL
                     INEPEVILADEPTGNLDSKTGREVMRQILTIQKNLSATLILVTHDPEIGAVGERKLTM
                     VDGELFTDQ"
     gene            complement(825..2024)
                     /locus_tag="PKN85_03455"
     CDS             complement(825..2024)
                     /locus_tag="PKN85_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNT43490.1"
                     /translation="MDYETTIGLRYLRSKRKEAFISFTTWISVVGIAIGVMAIIVVIA
                     VMTGFQDEIRARILGINPHILVLDVNGEIRDPATVVAKVKKVDGVVEAFPFTAFQAMA
                     QNGKQFSGVAVKGIDPGDAKFMAKLVKEGSVDVLQRKGSVLMGKELAKHLGLFIGDSF
                     TIMVPFGGFSPMGSMPETVRGRVGGIFETGMYEIDNTLVVMALSDVESAMGVGATGIE
                     IKLIDEYRATDLKWTILKTLGRDYFAKTWIEMNRNLFSALKLEKLAMFIILALIILVA
                     SFNIISSLIMTVMEKKKDIAILKSIGAKKRSIMKIFMMEGITIGVVGALFGSLTGYFL
                     CWIIKSTKLIRLPEDVYYITTLPAKISIVDVALIALVTTVICVLSTIYPSYKAAKIDP
                     VETLRYE"
     gene            complement(2180..3187)
                     /locus_tag="PKN85_03460"
     CDS             complement(2180..3187)
                     /locus_tag="PKN85_03460"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNT43491.1"
                     /translation="MSTIAHAIRVRGLEICPPLLQAPMAGLTHSALRQIVSGFGGAGL
                     LSTEMLLASRLPTENPGISPYLVRTAREKPLSYQLLISGDMDVGPAVDKLHDLGADAI
                     DLNMGCPDWSVGRSGAGFALMENPESARRLVMGARRRTDLPLTAKIRLGVGLDEPRLK
                     DFCAMLEGEGIDMVSVHARLKKEPFARRPRWEWVARIKEWLRIPVIANGGIFTVEDAG
                     DCLSVSGADGLMLGRGAVARPWLFADIAREVYGCDIAAPRVSLPLLYGTFTDLIKELF
                     PPERRLGRLKEFTHHFARNYQFGHQLSSRIQGSRTMTQAEERSADFFKNNPECPGEKN
                     T"
     gene            complement(3184..4485)
                     /locus_tag="PKN85_03465"
     CDS             complement(3184..4485)
                     /locus_tag="PKN85_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43492.1"
                     /translation="MRFAAKACAHPAVFRVVLKEGAGIDVASYYETRCALEAGAPPVK
                     LDLNGNCKEDFLIARAIETDMLIVADSIEEFQIVSGMAKQMGKSPRVVVRLSGFELGH
                     VTAEAVFTAGRWTKFGACLDDMPRFLKTLNSHPHIRFLGFHTHIGSQIAELAPYLAVL
                     GKMIELGHLLTQTGRKCEIINIGGGFPLSYVTKEEWDRFTERVKDGYLASLTGDMSRV
                     FIWNNRTGGFERRPDGSIDASRWNDDTFYTAYPKEKMVEAILRGKVPVGGKDVDTVRA
                     LKDLGEPDLVIEPGRGVVGDSAVTLARVSQVRRVGKWHNLVSLEMGVTSFGEALVYMP
                     VNRWEIINDRDRRDPEPFEAFVAGNLCFSGDMLARYKVSLQRRPVRGDVVLIHDTGSY
                     GPQFFASNANSFPRPARVLVETGGKLTVMRQRDSYNDIFSL"
     gene            complement(4486..>4715)
                     /locus_tag="PKN85_03470"
     CDS             complement(4486..>4715)
                     /locus_tag="PKN85_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43493.1"
                     /translation="LGSGVGASSARPVTGSDAGDGEFVKPSGKEAHIAERSLSWWIER
                     YKLPLHVYSADVIARNVRAFGKVFRDLYPKG"
BASE COUNT          882 a         1426 c         1444 g          963 t
ORIGIN      
        1 actttggtgt ccactaaagg catcataggt cacaggaaac aacagatgca ggctacgatg
       61 agcggctcaa gtcgacgcaa ccgtctcccc tgtctttgtc tttaaccctc aagcgactca
      121 agcgattcaa gccactcaag ccggttttct attgatcggt aaagagctcc ccgtcgacca
      181 tcgttagctt tctttcgccc accgcgccga tctcggggtc gtgggtcacg aggatgaggg
      241 ttgcggagag gttcttttgg atggtgagga tctggcgcat gacctcgcgg cctgtcttgc
      301 tgtcgaggtt gcccgtcggt tcgtcggcga ggatcacttc gggttcgttg atgagcgccc
      361 tggcgatggc gacgcgctgc tgttcgccgc cggatagttc cccgggtttg tggttcaggc
      421 ggtgggtaag ctcgacggtc tcgaggaagt tccgcgcctt cgcttcggcc tccgcgggct
      481 tcatgcgccg tatgaggagc ggcatcatga cgttctcgac gacgttgagg tcctgcagga
      541 ggtggtagaa ttggaagatg aaccccacct tctcgttgcg gaaacggctc tgctcgtcct
      601 cgctgaagga catgatatcc tgaccgccga agaggaccgt gccctccgtc ggcttgtcca
      661 gggtgccgat gatgtggagg aaggtgctct tgccggcacc ggaggggccc atgatggtaa
      721 tgaactcacc tttgtccgcg gtgaagtcca cgcccttcaa gacctcgatc acgatgtcgt
      781 ccttctggaa agaccttttc agcgccgaga cttcgatgat gcgatcactc atagcgcagg
      841 gtctccacgg ggtcgatctt cgccgccttg taggaggggt agatcgttga aaggacgcat
      901 atgaccgtcg tgacgagggc gatgagcgcc acgtcgacga tgcttatctt cgcgggcagg
      961 gtggttatgt agtagacgtc ctcggggagc cggatgagct tcgtgctctt gattatccag
     1021 cagaggaagt acccggtcag cgaaccgaag agggcgccga cgacgccgat ggtgatccct
     1081 tccatcatga agatcttcat gatgcttcgc ttcttcgcgc cgatggattt cagtatggcg
     1141 atgtccttct tcttctccat gacggtcatg atcagcgagc tgatgatgtt gaagctcgcc
     1201 acgaggatga tgagggcgag gatgatgaac atcgccagct tctcgagctt caaggccgag
     1261 aagaggttcc ggttcatctc gatccatgtc ttcgcgaagt agtcccgtcc cagcgtcttc
     1321 aggatcgtcc acttgaggtc ggtcgccctg tactcatcga tgagcttgat ctcgatgccc
     1381 gtcgcaccca cacccatggc cgattccacg tcggaaagcg ccatgacgac aagggtgttg
     1441 tcgatctcgt acattcccgt ctcaaagatg ccgcccacgc ggccgcgcac cgtctccggc
     1501 atggagccca tgggggagaa accgccgaag gggaccatga tggtgaagct gtcgccaatg
     1561 aagagaccga ggtgcttcgc caactccttc cccatgagga cgctcccctt tctctgcagg
     1621 acatcgacgg agccttcctt caccagcttc gccatgaact tcgcgtcacc ggggtctatc
     1681 cccttcaccg cgacgccgga gaattgtttc ccgttctggg ccatcgcctg aaaggccgtg
     1741 aaagggaagg cctccaccac tccgtccacc ttcttgacct tcgcgacaac cgtggcgggg
     1801 tcgcgtatct cgccgttgac gtcaaggacg aggatatggg ggttgatgcc gagaatgcgc
     1861 gccctgatct cgtcctgaaa accggtcatg acggcgatga ccacgattat ggccatgacg
     1921 ccgatggcaa tgccgacgac ggatatccac gtcgtgaagg agatgaacgc ctccttgcgc
     1981 ttcgaccgca ggtaccgcaa ccctatcgtg gtctcgtagt ccatgaaagt gctaatgtct
     2041 taccacaagg cgggggaaaa gggaaggaca gtgaggacct gttacgggtt gcgggttgcg
     2101 ggttgcgggt tcaggaaacg gtgtccacct gaaggtctcc tggtgctgcc tgctccggag
     2161 cgtgcgaact gacaggaggc tatgtgtttt tctctccggg gcattcgggg ttgtttttga
     2221 agaaatcggc ggacctttcc tcggcctggg tcattgtgcg gctgccctgg atgcgggagc
     2281 tgagctggtg gccgaactgg tagttacgtg cgaagtggtg ggtgaattcc ttgagccttc
     2341 cgagtcggcg ctcgggcggg aaaagctcct ttatgagatc tgtgaacgtg ccgtagagca
     2401 ggggcaaaga gacgcggggc gcggcgatgt cgcagccata gacctcccgg gcgatgtcgg
     2461 cgaagagcca gggcctggcc acggccccgc ggccgagcat gagtccgtct gcgcccgaca
     2521 cgctgaggca atccccggcg tcctccaccg tgaatatgcc cccgttggcg atgacgggga
     2581 tgcggagcca ttcctttatc ctggcgaccc actcccatcg cggccttctc gcgaagggtt
     2641 ccttcttcag ccgggcgtgg acggaaacca tgtcgatgcc ctcgccctcg agcatggcgc
     2701 agaagtcttt caggcgtggc tcgtcgagcc cgacgccgag acgtatcttc gccgtgagcg
     2761 gcagatccgt ccttcttctt gcccccatca ccagacgccg cgcactttcg ggattctcca
     2821 tcaaggcaaa ccccgcgccc gacctcccca cggaccaatc gggacagccc atgttgagat
     2881 cgatcgcgtc ggctccgaga tcgtgaagct tgtccacggc cgggccgaca tccatgtcgc
     2941 cggaaatgag gagttgatag gacaggggtt tctcccgggc cgtcctgaca aggtaggggg
     3001 atatcccggg attctcggtg ggcagtctgc tggctagcag catctccgtg gagaggagac
     3061 cggccccgcc gaatcccgag acgatctgcc tgagggcgct gtgggtgagc cctgccatgg
     3121 gggcctgaag caggggcgga cagatctcta ggccccgaac gcggatggcg tgcgctattg
     3181 tgctcacagg gaaaagatgt cgttgtaact gtcccgttgt ctcatcaccg tcagcttgcc
     3241 gcccgtctct acgaggacgc gggcggggcg gggaaaggag ttcgcgttgg acgcgaagaa
     3301 ctgggggccg taggagccgg tgtcgtggat gaggaccacg tcacccctta cgggtcttct
     3361 ctgcagggag accttgtacc ttgcgagcat gtcgcctgag aagcagaggt tgcccgccac
     3421 gaaggcctcg aagggctccg ggtccctgcg gtcgcggtcg ttgatgatct cccagcggtt
     3481 gaccggcatg tacacgaggg cctctccgaa gctggtcacg cccatctcga ggctcacgag
     3541 attgtgccat ttcccgaccc tcctcacctg ggacacccgc gcgagggtga cggccgagtc
     3601 tcccacgacg ccgcggcccg gttcgatgac gagatcgggc tcccccaggt ccttgagggc
     3661 ccggacggta tcgacatcct tccctccaac gggcaccttg ccccggagta tggcctccac
     3721 catcttctcc ttgggatagg cggtgtagaa tgtgtcgtca ttccaccgcg aggcatcgat
     3781 cgaaccgtcg ggccggcgct cgaaaccgcc cgtgcggttg ttccatatga agacacggct
     3841 catgtccccc gtgagcgacg cgaggtagcc atccttcacc ctctccgtga acctgtccca
     3901 ctcctccttt gtcacgtagg agagggggaa accgccaccg atgttgatga tctcgcactt
     3961 cctgcccgtc tgagtaagga gatgcccgag ttcgatcatc ttgcccagga ctgcgaggta
     4021 cggggcaagc tccgcgatct gggagccgat atgggtgtga aaaccgagga aacggatatg
     4081 gggatgactg ttcagggtct taaggaaccg gggcatatca tcgaggcaag ccccgaactt
     4141 cgtccatctc cccgcggtga agacggcctc cgcagtaaca tggccaagct caaacccgct
     4201 gaggcgcacg acgacccgcg gactctttcc catttgtttc gccatcccgg agacgatctg
     4261 gaactcctcg atgctgtccg ccacgatgag catgtccgtc tcgatggcgc gggcgatgag
     4321 aaagtcctcc ttgcagttgc cgttgagatc gagtttaacc ggaggggccc ccgcctcgag
     4381 ggcacaccgg gtctcgtagt aggaggccac atcgatcccc gccccctcct tgagaacaac
     4441 gcggaacacc gccggatgcg cgcatgcctt cgcggcaaac cgcacctagc ctttcggata
     4501 gaggtcccgg aagaccttcc cgaaggcccg cacgttccgg gctatgacat cggcggaata
     4561 gacgtgcaaa ggaagcttgt accgctcgat ccaccacgag agggaacgct cggcgatatg
     4621 ggcctctttc cccgacggct tcacaaactc tccatccccc gcatcactcc ccgtcaccgg
     4681 acgagcgctc gacgcaccca cacccgatcc aagaa
//
LOCUS       DAOQTR010000098         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20186_length_4710_cov_3.903759, whole genome shotgun sequence.
ACCESSION   DAOQTR010000098 DAOQTR010000000
VERSION     DAOQTR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20186_length_4710_cov_3.903759"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="PKN85_03475"
     CDS             complement(<1..1672)
                     /locus_tag="PKN85_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43494.1"
                     /translation="MKDPSGTTRELIEEIASLKERIRELERLEGRRTEEALRESERKF
                     RDLAEKSIAGIYVLRPDGVVRYVNARCAEIFGYTVDEAIDRLSVRDVIFSGDLPLVEA
                     SIGKRTSGELPSHHYEFRIVTKTGDIRYVEVYSSYTVHEGKSAVIGTILDISERKRAE
                     NALMASEERFRTFFNLPIVGFAITGADSRWIHFNDKLCEMLGYSREELERTTWMRITP
                     SEDLARERPLFEDVVRGGRNTAGIEKRYVRKDGSLADVFVSTQVVRNPDGSVAYFASI
                     IQDITAQKRAEGEIIREREKLRTLSDNAPFGMVLIDEDGRFTYVNAKFTELFGYGLSD
                     IPDGRTWFRKAYPDAEYRHTVISTWLEDFEDTGPAERKPRVFTVTCNNGIKKMIEFIP
                     SKLASGDYLMTCEDITELVRLESQLRQAQKMESIGTLAGGVAHDFNNILTTLIGYASL
                     IQTKMDRDNPLRSYVDQVLSASRKGADLTRSLLTFSRQQPVTLLPVDLNDAIRATEPL
                     LKRLLTEDIELTTSLTGEDTVVMADRSQMDQILFNLVTNARDAMPGGGR"
     gene            complement(1962..2234)
                     /locus_tag="PKN85_03480"
     CDS             complement(1962..2234)
                     /locus_tag="PKN85_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43495.1"
                     /translation="MSTRSLTMLLALAALMSVASCASFDCAYDVKARPEPVYLAAEEG
                     VFYGQNYTACFVIDGVVHKEYIPECAYRYYYDRQNQRRTGAPATRQ"
     gene            complement(2398..2670)
                     /gene="hgcB"
                     /locus_tag="PKN85_03485"
     CDS             complement(2398..2670)
                     /gene="hgcB"
                     /locus_tag="PKN85_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_250697394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HNT43496.1"
                     /translation="MVYLKDVVTLTHIPELCSGCGICLSVCPREVLQRLNGKVRIARR
                     DACIECGACQRNCPHGAVTVKAGVGCASALINQMLGRKKACCVVGD"
     gene            complement(2690..3808)
                     /gene="hgcA"
                     /locus_tag="PKN85_03490"
     CDS             complement(2690..3808)
                     /gene="hgcA"
                     /locus_tag="PKN85_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_252989029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3758..3760),aa:Sec)
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HNT43497.1"
                     /translation="MKPLYSSLVMLDNAPCUGPSEKRGPVRPPRLDQPFVTGVIKTAA
                     GPVPRVSKVFSGTDRIGAFKVRWGIRRTRYRVDAGLYAIGEPTAESPVFVTANYKLTF
                     DLLRRAIASIDGWILVLDTLGINVWCAAGKGTFGTDELVERIEASRLAGIVNHRKLIL
                     PQLGGPGVAGHEVKRRSGFQVVYGPVEAEEIPLFLEKGLKAKERMRRKDFGFMERLAL
                     VPMEVVPAVRLGLPVAAGLFALCFLLGGLSLSRALECFAVPAGAIGTSILAGTVLTPV
                     LLPWLPGRAFSVKGLVTGILSALLLIPLVRGSGVLETAFLLVSIPAMSAYLAMNFTGS
                     STFTSLSGVKKEMRFALPLEIGGVGAGLLMWIIALLGG"
     gene            3952..4320
                     /locus_tag="PKN85_03495"
     CDS             3952..4320
                     /locus_tag="PKN85_03495"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT43498.1"
                     /translation="MKELLSVFKALSDETRLRIVKLLENGELCVCHIVAAVDMSQSRV
                     SFHLKILKDAGLVKDRREGKWMHYRLNESDLFKRLLFLSIVERVKEEDMAGDRQRLEA
                     FIASRPEEFAGISTACCGRV"
     gene            4528..>4710
                     /locus_tag="PKN85_03500"
     CDS             4528..>4710
                     /locus_tag="PKN85_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HNT43499.1"
                     /translation="MSFYMSARVKAHCGQAATLSFTAASNNFELAIAVAVAVFGIDSG
                     QAFAAVIGPLVEVPVLV"
BASE COUNT          963 a         1368 c         1351 g         1028 t
ORIGIN      
        1 gcctcccgcc cccgggcatg gcgtccctgg cgttcgtgac gaggttaaag agaatctggt
       61 ccatctgcga cctgtcggcc atgaccaccg tgtcctcgcc ggtgagcgac gtggtgagtt
      121 cgatgtcctc ggtgaggagt ctcttcaaca atggttccgt tgctcgtatc gcgtcgttga
      181 ggtccaccgg gaggagagtg acgggttgct gtctgctgaa ggtcagaagg ctccgcgtca
      241 ggtctgcacc cttcctcgag gccgagagga cctggtcaac gtatgagcgc aaggggttgt
      301 ccctgtccat ctttgtttgt atcagggagg cataccctat gagggtggtg aggatgttgt
      361 tgaaatcatg cgcgacacct cccgcaaggg tcccgatgga ctccatcttc tgggcctggc
      421 gcaattgcga ttccagccgt accagttcgg tgatatcttc gcaggtcatt agataatccc
      481 cggaagcaag tttcgaaggt atgaactcga tcatcttctt tatcccgttg ttgcatgtaa
      541 cggtgaacac cctcggtttc cgttcggcgg gtccggtgtc ctcgaagtcc tcaagccagg
      601 tcgagatgac cgtatgccgg tattcggcat cagggtaggc ctttctgaac caggtcctgc
      661 cgtccgggat atccgacagc ccgtacccga agagctccgt gaacttcgcg ttgacgtatg
      721 tgaaacggcc atcctcgtcg atgaggacca ttccaaaggg ggcattgtcc gaaagggtcc
      781 tcagtttttc cctttccctg atgatctctc cctcagccct tttctgggcc gttatgtcct
      841 gtatgatgga ggcaaaatag gccaccgaac catcggggtt acggaccacc tgggtggaga
      901 cgaagacatc ggcaagggaa ccgtccttcc tgacatatcg tttttcgata ccggcggtgt
      961 tccttccgcc ccttaccaca tcttcgaaga gaggtctttc cctggcgagg tcctcggatg
     1021 gtgtgatccg catccaggtc gttctttcca gttcctctcg ggaataccca agcatttcgc
     1081 aaagtttatc gttgaaatgg atccacctgc tgtcggcccc ggttatcgcg aaacccacga
     1141 tgggaaggtt gaagaaagtg cggaaccgtt cctcgctcgc catcagggcg ttctcggcac
     1201 gcttgcgctc ggaaatatcc agaattgtcc cgataacggc cgattttcct tcatgcacgg
     1261 tgtaggagct atagacctcg acgtacctga tgtccccggt cttcgtgact atcctgaact
     1321 cgtaatggtg tgacggcagt tctcccgagg tcctcttgcc gatgctggcc tctacaaggg
     1381 ggaggtcccc ggagaatatc acgtccctca cgctcagccg gtcgatcgcc tcgtcgacgg
     1441 tgtagccgaa tatttcggca caccgcgcgt tgacgtaccg caccacccca tccggccgca
     1501 ggacatagat accggcgatg gatttctcgg caaggtcccg gaacttgcgc tccgattccc
     1561 tgagcgcctc ctcggtgcgc ctgccttcca gacgctccag ttccctgatc ctctccttta
     1621 aagaggctat ctcttcgatc aattcccggg tcgttcccga cgggtctttc atgcgtcccg
     1681 tttcacgagt ccgacgatgc actgatgtaa aggcatacac gatgttatca tagacagcgt
     1741 tgttgtcaat tcccaaatct ccgtaaagaa cgatcgcgtg tcgcggcagc ggtcggctgt
     1801 tgacgtgacg tcctctccgt tatggttgtc gcgcgatgtc cccggagcat gagaggtggg
     1861 gaaaggtcgc cgaaagacaa ctgccccggc ctctgattcc cgagaacctc aaaaggtagc
     1921 cggatgcgaa gatggaaggg atgtccccga aagcaccggc gtcattgtcg cgtggcaggt
     1981 gcgccggtcc gtcgctggtt ctgcctgtcg tagtaatatc ggtaagcgca ttcgggtatg
     2041 tactccttgt ggacgacgcc gtctatcaca aaacacgccg tgtagttctg cccgtagaag
     2101 acgccttcct cggcggcgag gtaaaccggt tccggacgcg ccttgacatc gtacgcgcag
     2161 tcgaatgaag cgcaggacgc gacagacatc agggctgcca gcgccaacaa cattgtcaat
     2221 gatctggtag acatggttcc acctccggac atatctctat tctatcaaat atccgatgca
     2281 ggggtaacca cggccttccc ggaacaagcc atgacggggc gacagggaca actgcggccg
     2341 cgtttgcccg ggatggaggt tgggggaacg acgcggtgat catccacgct ctgtccgtca
     2401 atctccgacg acgcaacagg ccttttttct gccgagcatc tggttgatga gggccgaggc
     2461 gcagccgacg cccgctttga cggtcaccgc tccgtgaggg cagttgcgct ggcaggcgcc
     2521 gcactcgatg caggcgtcac gccgggcgat cctgaccttt ccgttgaggc gttgaagcac
     2581 ctcacggggg cacacggaaa ggcagatccc gcagccactg cacaactccg gtatgtgggt
     2641 cagcgtgaca acgtctttga gatagaccat gggtcgcatt cttgtccctc taccctccca
     2701 ggagagcaat gatccacatc aggaggcctg cccctacgcc cccgatctcg aggggaaggg
     2761 cgaagcgcat ctccttttta acgccggaaa gcgaagtgaa tgtggaggaa ccggtgaaat
     2821 tcatggcgag ataggcagac atggcgggaa tagagacaag aagaaatgct gtctccagca
     2881 cacctgagcc gcgaacaagg ggaatgagca gaagtgccga caggatgccc gtcacaagcc
     2941 ctttaaccga aaaggcccgg cccggaagcc agggaagcag gaccggagtg agcaccgtgc
     3001 ccgcgagtat ggaagttccg atggccccgg cggggaccgc aaaacattcg agagctctcg
     3061 atagcgaaag ccccccaaga aggaagcaaa gggcgaagag acctgccgca acgggaagcc
     3121 ccaacctcac ggccggtacg acctccatcg gaacaagcgc cagtctttcc atgaagccga
     3181 agtccttcct ccgcatccgc tctttcgcct tgaggccttt ttcaaggaat agaggtatct
     3241 cttcagcctc aacgggtccg tagaccacct gaaagccgga gcgccttttc acctcgtggc
     3301 ccgcgacccc cggtccccca agctggggaa gtatgagctt ccggtggttc acgataccgg
     3361 caagacggct cgcttcgatg cgctccacca gttcgtcggt cccgaaggtg cctttccccg
     3421 cggcacacca cacgttgatc cccagcgtgt cgagtacgag gatccatcca tctatcgacg
     3481 cgatcgccct tcggaggaga tcgaaagtca gcttgtagtt cgccgtgaca aagacgggag
     3541 attccgcggt cggttcacct atcgcgtaaa ggcccgcgtc gaccctgtac cgcgtccgcc
     3601 tgatccccca tcgcaccttg aaggccccga tgcgatccgt tccgctgaag accttcgata
     3661 cccgggggac gggaccggcg gcggtcttga tcacgccagt gacaaaaggc tggtcgagac
     3721 gagggggtct caccggaccc cgcttctccg acgggcctca gcaaggcgcg ttatcgagca
     3781 tgacgagaga ggaatacaaa ggtttcatat aatccatgat agagcatgtg gaggctcctg
     3841 tcaaggcaat catatcaaga cacgttgaag tgttggaaaa taacacgttg acatatcagg
     3901 acaatcgtac tatatacata tctataatac ttgatatgaa gggaggccgg aatgaaagag
     3961 cttctgtcgg tgttcaaggc gttgtccgac gaaacgcggc tgaggatagt gaagcttctc
     4021 gagaacggcg aactctgtgt atgtcatatc gtggcggctg tcgatatgtc tcagtcgagg
     4081 gtttcctttc acctgaagat cctgaaagac gcggggctcg tgaaggacag gcgggaaggc
     4141 aaatggatgc attaccgcct caacgagtcg gacctgttca agcgccttct cttcctttcc
     4201 atcgttgaac gggtgaaaga ggaggatatg gcgggcgatc gtcaacgtct ggaagcattc
     4261 atcgcgtcac ggccggaaga gttcgccggc atctcgaccg cgtgctgcgg cagggtgtga
     4321 gcgtgggtgg tccggctgga agactttcct tcttggatcg ttttctgacc ctctggatct
     4381 ttctggcgat gttcgccggc gttgcctggg gatacctcaa tcccggcgtg gttgacttct
     4441 ggaaccggtt ccagtccggg accacaaaca tacccatcgc tatcggtctt atcctcatgg
     4501 ctgatctact ttgtcgtcat gttcctcgtg tcgttctaca tgagcgccag ggtgaaagcc
     4561 cattgcggtc aggccgccac gctttccttt accgcggcgt cgaacaattt cgaacttgcc
     4621 attgccgtcg cggtggccgt cttcggcatc gattccggtc aggccttcgc ggcggtcatc
     4681 ggacccctcg ttgaagtccc cgttctcgtc
//
LOCUS       DAOQTR010000099         4696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20282_length_4696_cov_3.098901, whole genome shotgun sequence.
ACCESSION   DAOQTR010000099 DAOQTR010000000
VERSION     DAOQTR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20282_length_4696_cov_3.098901"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKN85_03505"
     CDS             complement(<1..247)
                     /locus_tag="PKN85_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43500.1"
                     /translation="MDREREEAIRERIRCSGKARTENAAAAGDLENIRERALEVFANY
                     RKTKAVIRKEVRKECASEIRHLENERSQREMVIKEKMM"
     gene            complement(268..942)
                     /locus_tag="PKN85_03510"
     CDS             complement(268..942)
                     /locus_tag="PKN85_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNT43501.1"
                     /translation="MSGNQLVINNAGPNPTIDVSETPVRLGVTALSIQETVRSRVPPD
                     NSKYDRNEKVDELYRRWSQSPASCPEDRYEAVVLLFWELYRSRIMAIAKKYRALSPVF
                     DDDDLQQIGLLGIFQALTRYEHAEHIDMRFSTYLEWSVRNVFQRTIGYTDKFVEIYDR
                     DDGFLRVISYREFLVRKKAIVAEGLQYIIRSRQCYLSDMHHPSDCDTPFCLTAAGHDE
                     WDESDD"
     gene            complement(1268..2356)
                     /locus_tag="PKN85_03515"
     CDS             complement(1268..2356)
                     /locus_tag="PKN85_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNT43502.1"
                     /translation="MRKCRDIEDRFTAYLDGYLETAEREIVEEHLSRCPDCLHKIEDL
                     KRIRAILADLDEVDPPPGLTAKILSRIHEEEKTKAGIFHRLFFPLHIKIPVQALATVF
                     VVVLTVYVFKSTLHETANLERPSAAPPAVETVPDAAKKQAAPAVKKEAQPGRRPGSIS
                     PKPAPPATQTPKKEAAAPPATAQAPAAAAQESPPLRAYKAAPHPAPAAERASSPERHT
                     DDYLSHSKAHPAPEAGRIKTRGTVMEEKGRAAGSAPMAPARAAVPDVRSRITMMSKDP
                     RQTAIQVKTIMRSLGATGIDESAADNVATVSGWIDAAALKELMARLKEIASVDERNSS
                     LLSGPKSSFVEIAIRGSGNDDAVKPVDR"
     gene            complement(2353..3045)
                     /locus_tag="PKN85_03520"
     CDS             complement(2353..3045)
                     /locus_tag="PKN85_03520"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT43503.1"
                     /translation="METIALIWHNIISLIARDGTKPLAGLSHTKEGRHENRDEDAQYV
                     ALCQGGEPAAFEVLVRRHQKRMFNIAYRMTGNYEDAGDVVQEAFLSAYRTIKHFRGEA
                     MFSTWLYGIVVNHARNRMRQTGSRSYHEPVSLDDPPAGGEGGPAFDPPSRQTPALDVL
                     VRKETQEKVQGCINGLEKEHREIIVLRDIQGFSYEEITGMLGIPDGTVKSRLSRARNA
                     LKEGLKKALGDL"
     gene            3226..4107
                     /locus_tag="PKN85_03525"
     CDS             3226..4107
                     /locus_tag="PKN85_03525"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT43504.1"
                     /translation="MIADFAKKLETVINFIPPIPVVIVELLKALNDENANMNDIARII
                     AKDPPMSMNVLKVANSAFYRLTNKVATVDHAVRMLGVKELGMICVSCGAYQALKPPAD
                     SETFDINEFWKHSVATGVIAKKICAELSVGDKNTIYFGGLLHDIGKVVLDRFAHEVYK
                     IVIQVTHEECLPMIEAEKRIIGESHDTIGGMIMERWKFPFALADVARYHHAIALCPEE
                     TRPTVAVCALADELARVRYFGVGGDRSGIVLEETEAFRSLHTISPTIANVDVFKFLCD
                     LETVDEEIVEMEKILTT"
     gene            4305..>4696
                     /locus_tag="PKN85_03530"
     CDS             4305..>4696
                     /locus_tag="PKN85_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT43505.1"
                     /translation="MAIRKGKTQKALIRCAGSLLLVALFFLYACNVVYLEKFAQFDAH
                     QGNYEEAVQDLTKAIRYAPRDPVPRAARAYLYQKMGRHDEAIADYTKAIELDPADDEL
                     YLMRGLAYSLKGDQAKAILDYSTAMEIDP"
BASE COUNT          904 a         1388 c         1351 g         1053 t
ORIGIN      
        1 ccatcatctt ttccttgatg accatctctc tttgtgaacg ctcgttttcg agatgacgga
       61 tctcgcttgc acattccttc ctgacctcct tccggataac cgcctttgtc ttcctgtaat
      121 tggcgaagac ctccagcgct ctttccctga tgttttctag gtctccggca gccgcggcgt
      181 tttctgtacg ggctttcccg gagcacctga tgcgttcccg gatcgcttct tctctttctc
      241 tatccatcgg actttcgcct ttcatgatca gtcgtcgctt tcatcccact cgtcgtgtcc
      301 ggctgctgtc aggcagaagg gcgtgtcgca gtcggaaggg tggtgcatgt ctgaaaggta
      361 acactgcctc gacctgatga tgtattgcag cccctcggct acgatggcct tcttcctcac
      421 caggaattcc cggtaactga tgacgcgcag gaaaccgtca tctctgtcgt agatctcgac
      481 aaacttatcg gtgtacccga tggtgcgctg aaagacattt cgcacggacc attcgagata
      541 cgtggagaat cgcatatcta tgtgttcggc gtgctcgtac ctggtcaatg cctggaagat
      601 cccgagaagc cctatctgct ggagatcgtc atcatcgaag acggggctca aggccctgta
      661 cttctttgct atcgccatga tccttgatct gtagagctcc cagaagagga gaacgacggc
      721 ctcataccgg tcttcagggc agctggcggg cgattgcgac catcgacgat agagctcatc
      781 gaccttctca ttcctgtcgt acttgctgtt atccggggga acccgggacc gtaccgtctc
      841 ctgtatggaa agagccgtca ccccgagtcg taccggtgtc tcggaaacat cgatcgtcgg
      901 gttgggaccc gcgttattga taaccagttg gttgcctgac atattcaatg tgctcaacat
      961 cagggcggaa cgccagagcc ggtctttccg acacgcgtct tcatgcttgc gacacaccgt
     1021 gatgcgggta gtgaaaccgc gagccgcccc cgactcgccc ataccttaca tggctgttcg
     1081 aaaagttgtc aacaggaaat acacgagcct gcccatttgt attgcgacgc ccgtgagcgc
     1141 gacgcttttc gcagaagatc ctctgtgtga agggtcggaa atgacggtat gtgttgagga
     1201 tcggctatca ggcaggggac ctcatccgct tcgtcctgtc gagggctctg ccgctgcggg
     1261 aggagcatca cctgtcgaca ggtttcacgg cgtcgtcgtt cccagatccc cgtatggcga
     1321 tctcgacgaa agaggacttc ggacctgaca ggagggaact gttccgctcg tcaaccgaag
     1381 cgatctcctt cagccgggcc atgagttcct tgagggcggc tgcgtcgatc catcccgaga
     1441 ccgtcgcgac gttgtcggcc gcgctctcgt cgatccccgt ggctcccaga gagcgcatga
     1501 tcgtcttcac ctggatcgca gtctgacggg ggtccttcga catcattgtg atgcggcttc
     1561 ggacgtcggg caccgcggcc cgggcgggag ccatgggcgc ggaccctgca gccctgccct
     1621 tctcctccat caccgtgccc ctcgtcttga tacgccctgc ctcgggtgcc ggatgggcct
     1681 tcgaatgcga caggtagtca tctgtatgcc gttcggggct cgatgccctc tccgcagccg
     1741 gggcgggatg cggagctgct ttgtaggcgc ggagcggcgg cgactcctga gcggcggcgg
     1801 cgggcgcctg tgccgtcgcc ggcggagcgg ccgcttcctt cttcggggtc tgtgtcgcgg
     1861 gcggcgcagg tttgggagag atggatccgg gtcttctccc gggctgcgct tccttcttca
     1921 cggcaggtgc cgcctgcttc ttcgctgcat ccggcactgt ctccacggcg ggaggagccg
     1981 cggaaggcct ctcgaggttt gccgtttcgt gaagggtcga cttgaaaacg taaacggtca
     2041 ggacgacgac aaacacagtc gccagtgcct gcacggggat ctttatatgg agggggaaga
     2101 agagcctgtg gaagatgccc gccttcgttt tctcctcttc gtggatccgc gacagtatct
     2161 ttgccgtcag gcccggtggg gggtcgacct catcgaggtc ggccagtatc gcccgtatcc
     2221 tcttcagatc ttcgatctta tgaaggcaat cggggcacct ggaaagatgc tcctccacga
     2281 tctcgcgttc cgcggtctcg agataaccgt caagataggc ggtgaaacgg tcctctatgt
     2341 cgcggcattt tctcataggt caccgagcgc ttttttcaag ccttccttga gggcgttgcg
     2401 tgccctcgaa agccgggact tgaccgtgcc gtcgggtatg ccgagcatcc cggtgatctc
     2461 ttcgtaggag aacccctgga tgtccctgag gacgatgatc tcccggtgtt ccttttcgag
     2521 gccgttgatg cagccctgca ccttctcctg ggtctccttc cgcacgagca catcgagggc
     2581 gggggtctgc cgcgagggcg gatcgaaagc gggaccgcct tctcctccgg caggcggatc
     2641 gtcgaggctc accggttcat ggtacgacct gctccctgtc tgacgcatgc ggttcctggc
     2701 atggtttacc acgatgccgt agagccaggt cgagaacatg gcctcaccgc gaaaatgctt
     2761 tatcgtcctg tatgcggaga gaaaggcctc ctgcacaacg tcgcctgcgt cttcgtaatt
     2821 tcccgtcatc cgatacgcta tgttgaacat cctcttctgg tgtctcctca ccaggacctc
     2881 gaaagccgcc ggttcgcctc cctggcacag cgcgacgtac tgagcgtctt cgtccctgtt
     2941 ctcatgccgc ccttccttcg tatgagacag cccggcgagt ggttttgttc cgtccctggc
     3001 tatcagggat ataatgttgt gccatatgag ggctatagtt tccattcggc cgcgacgcga
     3061 tgaggtagat ttttagatgt tttacagcac cgtgacgtcg attgcaaaga aaaaaggttg
     3121 ccgccaggcc gccaggccgg gagaccttaa gagatgatgc ctttatccga tgatactatt
     3181 accggcagag atggtcctgc gggaatccat ggaggtctgg gatctgtgat agcggatttc
     3241 gccaagaagc tcgagacggt gatcaacttc atccctccca ttccggtcgt catcgtcgaa
     3301 ctcctgaagg cgctcaacga tgagaacgcg aacatgaacg acatcgcgcg gataatcgcc
     3361 aaggacccac cgatgagcat gaacgttctc aaggtggcca attccgcatt ctaccgcctc
     3421 acgaacaagg ttgccaccgt ggaccatgcc gtgcggatgc ttggcgtgaa ggagctggga
     3481 atgatctgtg tctcctgcgg ggcgtatcag gccctcaagc cgccggcgga ctcggagacc
     3541 ttcgacatca acgagttctg gaaacactcc gtcgcgacgg gtgtgatcgc gaagaagatc
     3601 tgcgcggagt tgtccgtcgg cgacaaaaac acgatatact tcggcggtct cctccacgat
     3661 atcggcaagg tggtcctcga ccgctttgcc catgaggtct acaagatcgt catccaggtc
     3721 acgcacgaag aatgtcttcc catgatcgag gccgagaaga ggatcatcgg cgagtcgcac
     3781 gacaccatcg gcgggatgat catggagagg tggaaattcc ccttcgccct cgccgacgtg
     3841 gcgcggtatc atcacgccat tgccctgtgc cccgaagaga cgcgcccgac agtcgccgtg
     3901 tgtgccctcg ccgacgaact ggcgcgggtg aggtacttcg gtgtcggcgg tgacaggagc
     3961 ggcatcgttc tcgaagaaac cgaggccttc cggtccctgc acaccatctc gcccaccatc
     4021 gcgaacgtgg acgtcttcaa gttcctgtgc gacctcgaaa ccgtcgatga ggagatcgtc
     4081 gagatggaaa agatactgac aacctgaaaa aaggttcaaa ggttcaaagt tccaggttcc
     4141 aggctaaaga ccctcacagg gagcagctga tagtacgacc aggaccaata ggacctacag
     4201 gcgctatacg tcccatccag cctacccctt cccttttgct ggtgaaaaac ctgcgaccgc
     4261 gtgatagaat gaggctcaaa taccatcaga caggagaaga caccatggct atccgaaaag
     4321 gaaagacgca gaaggccctg atccgctgtg ccggttctct tctgctggtc gccctctttt
     4381 ttctttatgc ctgcaatgtt gtctatctcg agaagtttgc ccagttcgac gcccatcagg
     4441 ggaactacga ggaggccgtg caggacctca cgaaggccat ccgctacgcg ccgagagatc
     4501 cggtgccccg ggcggcgcgc gcgtatcttt atcagaagat gggcagacat gatgaggcca
     4561 ttgccgatta cacgaaggcc atcgagctcg acccagcgga tgacgagctc tatctcatgc
     4621 ggggccttgc gtactccctc aaaggggatc aggccaaggc gatactggac tactcgacgg
     4681 ctatggagat agaccc
//
LOCUS       DAOQTR010000100         4677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20434_length_4677_cov_2.861532, whole genome shotgun sequence.
ACCESSION   DAOQTR010000100 DAOQTR010000000
VERSION     DAOQTR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20434_length_4677_cov_2.861532"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PKN85_03535"
     CDS             complement(<1..895)
                     /locus_tag="PKN85_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43506.1"
                     /translation="MSVLRSIPFASDIIEEIAGIIGNEASTGEETVIVFPGKRPSLYL
                     KARLAALAGPRAFFPPRCFSLDQLIDEIARRQNPGFADIDHGDAAFILFGLIRSLGAF
                     ERHALAGKGFGDFLHWGRHLLSFIDRLDMEGIENSALVNVERNASIGYDVPQSVNELL
                     SNISLIRSEFHRVLAEGRWFTRGTKRIAAVKETDGRVPDDLRRVVFAGLFGLTGGERK
                     ILRSFWDAGQTTVLLSGDPEDWPVLKDLVGHLKAKVEYGDAARNERPVVNLHAGHDTH
                     AEVLEAYRILKESPRKKTAIVL"
     gene            complement(892..4242)
                     /locus_tag="PKN85_03540"
     CDS             complement(892..4242)
                     /locus_tag="PKN85_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT43507.1"
                     /translation="MADDRTRGTVPQIITVRSSAGSGKTYRLAEHYLKVLLAAALADT
                     TLPTRMANIVAITFTNKAAQEMRTRILEWMKRIILDLPFENSSLKPLDTIMKGITTVL
                     PDAPLPDRGASDALQEAGDLTARALKVRDYLMGAMDRRFTELLKDFGRFNVSTIDSFV
                     NLTLKASAMQLNLPPDFEVSTETKELIDLALRECLQRTGEDGRARAIFDAFLESYIEL
                     EGDRAGWVPRDLLGAAISSLWNEETKENRQFVLPAGGAHAGAAALRASVADSASRLLT
                     ALADDTGLKPLSHLLAALKRCADPRENALSKSVYFERSLEDCLKKGSDPPGPEERELW
                     DTVVRLRQLYAGALAVSKYAPYLQVYSFFKEVFQREITYYRRVIPIEELNRLLQDIVS
                     RRDFVPEIYYTLSERYIHFLLDEFQDTSLLQWKNVEVLAEEALSRGGSLFLVGDRKQA
                     IYRWRGGRPELVDDIAERYQDLYRVEELGLDTNYRSSEHIVAFNNAVFDAENLAVLAG
                     SVLGGHPPEAAREAVKPYRTSRQHFLEGKKGMGLVSIERLTAEGDDENGQDVFTGDEA
                     RQLVEERLKTLVQGIMARGVFRQGDLAVLVRTREEAGAVAAVLLSMGLSVESEHTVSI
                     RNNPLIREVICFLKFLDKPDDDLSFASFITGRIFAAKNGDWAARMPAWLSERYLTDRD
                     RFLYLQFKSDHGAVFEEGFASFLSRANYLPLYDLVVNFYRHWEVLVRFPDDVPYLLHF
                     LELVKERERSDMSSLAGFIDFLEESPRGVSYGASNDDKAFLLATPDSLNAVKVLTIHK
                     AKGLQFPVVILPFVTLTSFSASSGRDKQRCFQCDDDGLRLFHLKKEYTDVSPELAALY
                     RTKEREYLSDELNNLYVAFTRPEEELYVLLTDKKRQRNHLIDNIFGMEAFRPFANDRS
                     ITLGEPRKAARPGKDAATPRPGPAASFDDFTGEPAWAVKLQGSIADPGQLSRRAVRAR
                     RKGDAIHRALSFIASLPVDDETIASLARAAAIHEGIPDAAREVERSLKAFFMNRRFRA
                     FFETGPGRSFYNERAIVDARGSTFKMDRVIVDPGLVQVIDYKTGEIRSDTHVEQVSRY
                     GRLLSEVYPDREVRGFLLYLDDGEVVSI"
     gene            complement(4251..>4677)
                     /locus_tag="PKN85_03545"
     CDS             complement(4251..>4677)
                     /locus_tag="PKN85_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HNT43508.1"
                     /translation="ALDPAFSEQSFRETAEDLFFKIQGGWTRRDIGGIAGLLTPQTRD
                     IFQGDISRYLAEKKINRLENIAVRQVEIVDAVQDQGEEFVTVRYTASLLDFTTDETGT
                     VLSGSPTDPVKFLEYWTYTRKTGERDWKLAAITQEQDYR"
BASE COUNT          841 a         1387 c         1521 g          928 t
ORIGIN      
        1 gaagaacgat ggcggtcttc ttccgtgggg attccttgag gatgcggtat gcctcgagga
       61 cctccgcgtg cgtatcgtgg cccgcgtgaa gattgaccac cggccgttca ttcctggcgg
      121 catccccgta ttccaccttt gccttcaggt ggccgacgag gtctttgagc acaggccagt
      181 cctccgggtc gcctgagagg agcacggtgg tctgtccggc gtcccagaag gacctgagaa
      241 tcttcctctc ccctcccgtg aggccgaaga gcccggcgaa gacgacgcgt cgaaggtcat
      301 cggggacacg cccgtccgtt tccttcaccg cggcgatgcg cttcgtgccc ctcgtgaacc
      361 accggccctc ggccagaacc cggtgaaact cgctcctgat cagagaaata ttggacagaa
      421 gctcgttgac gctctgaggc acatcgtatc cgatggacgc gttcctctcc acgttgacga
      481 gggcgctgtt ctcgatccct tccatatcga ggcggtcaat gaaactgaga aggtgtctac
      541 cccagtgcag aaagtctccg aaccccttgc cggcgagagc atgccgctcg aaggccccga
      601 gggaccgtat gaggccgaag aggataaagg ccgcgtcccc atgatcgata tcggcgaatc
      661 cggggttttg cctccgggct atctcgtcga tcaactgatc cagggagaag caccggggag
      721 ggaagaaggc cctggggccc gcgagggctg ccagtcgggc cttgagatac agggaagggc
      781 gcttccccgg aaagacgatg accgtctctt cgcccgttga ggcctcgttc ccgatgatgc
      841 cggcgatctc ctcgatgatg tccgaggcga agggtatgga gcggaggaca ctcatatgct
      901 gacgacctcg ccgtcatcga ggtagaggag gaagcctcta acttcccggt cgggatatac
      961 ctcggagagg agtctgccgt accggctcac ctgttcaacg tgggtgtcgg agcggatctc
     1021 gccggtcttg taatcgatga cctgcaccag gccgggatcc actatcaccc ggtccatctt
     1081 gaaggtgctg cccctggcgt ccactatagc cctttcgttg taaaaagaac ggccggggcc
     1141 tgtctcgaag aaggcccgga agcgccggtt catgaagaag gccttcagtg atcgctcgac
     1201 ctcgcgggcc gcgtcgggta taccttcatg gatggcggca gccctggcaa gggacgcgat
     1261 ggtctcatcg tcaacgggca gggacgcgat gaaggagagc gcccggtgta tggcgtcacc
     1321 cttcctgcgc gccctgaccg cgcgtctcga gagttgcccc gggtcggcta tactcccttg
     1381 aagcttcacg gcccacgcag gctcgcctgt gaaatcgtcg aaagaggccg ccgggccggg
     1441 ccggggtgtg gcggcatcct tgcccggtcg ggcagccttc ctcggctctc ccagggttat
     1501 cgacctgtcg ttcgcgaaag gccggaaagc ctccatcccg aagatattgt cgatgaggtg
     1561 gttcctctgc ctcttcttgt ccgtgagaag gacgtacagc tcctcctcgg gccgggtaaa
     1621 ggcgacatag aggttgttga gctcgtcgga gagatattcc cgttcctttg tccggtagag
     1681 ggcggcaagc tcgggggaca catcggtgta ctccttcttc aggtggaaga gcctcaaccc
     1741 atcgtcgtcg cactggaagc acctctgctt gtcccggccc gatgacgcgg agaaggacgt
     1801 cagggtcacg aaggggagga tgaccacggg aaactggagc cctttcgcct tgtgtatcgt
     1861 gagcaccttc acggcattga gcgagtccgg ggtggcgagg agaaaggcct tgtcgtcgtt
     1921 ggaggcaccg tatgaaactc cgcgggggct ttcctccagg aaatcgatga agccggcaag
     1981 gctgctcata tcggagcgct ccctttcctt gacgagttcc aggaaatgca ggagataggg
     2041 cacatcgtcg gggaatcgga cgagcacctc ccaatgccgg tagaaattga caacaaggtc
     2101 gtaaagggga agataattgg cccgggaaag gaaagaggcg aacccttcct cgaagacagc
     2161 cccgtgatcg gacttgaact ggaggtacag gaagcggtcc cggtccgtaa gatatctctc
     2221 cgacagccac gcgggcatcc gggccgccca gtcgccgttc ttcgccgcga atatccgtcc
     2281 cgttatgaat gacgcgaagg aaaggtcgtc atcgggtttg tcgaggaact tgagaaagca
     2341 gatgacctcc cggatgagag ggttgttcct gatgctgacg gtgtgttccg actcgacgct
     2401 gagccccatg gacaggagga ccgccgcgac ggccccggcc tcctcccgcg tcctcaccag
     2461 aacggcgagg tcgccctgtc gaaagacgcc ccgcgccatt atcccctgaa cgagcgtttt
     2521 aagcctctcc tccacgagct gccgggcctc atcgccggtg aagacgtcct ggccattctc
     2581 gtcatctccc tccgcggtga gccgttcgat ggacacgagc cccatgccct tcttcccctc
     2641 caggaagtgc tgacgcgatg tccggtaggg tttcaccgcc tcccgggcgg cctcgggggg
     2701 gtgtccgccg aggaccgagc ccgcgaggac ggcaaggttc tcggcgtcga agaccgcgtt
     2761 gttgaacgcg acgatgtgct ccgaactgcg gtagttggtg tcgagaccga gttcctccac
     2821 gcggtagagg tcctggtaac gctcggcaat gtcgtcgaca agctccggtc gccctccgcg
     2881 ccagcgatat atcgcctgtt tcctgtcgcc cacgaggaaa aggcttccgc cccggctcaa
     2941 ggcctcctcc gcgaggacct cgacgttctt ccattgcagg aggctcgtgt cctggaattc
     3001 gtcgagaagg aaatggatgt atcgctccga aagggtgtag tagatctcgg gaacgaaatc
     3061 cctgcggctg acaatgtcct gaagcaggcg gttgagctcc tcgatgggga tgacgcgcct
     3121 gtagtatgtg atctcccgct ggaatacctc cttgaagaaa ctgtagacct gaaggtaggg
     3181 ggcgtatttc gaaaccgcca gggccccggc ataaagctgg cgcaggcgga ccacggtgtc
     3241 ccagagttcc ctttcctccg ggccgggcgg atcgctgccc tttttcaggc aatcttccag
     3301 ggagcgctca aagtaaacgc ttttcgaaag ggcgttctcc cgcggatcgg cgcacctctt
     3361 aagcgccgca agaaggtggg agaggggttt taggccggtg tcgtccgcca gggccgtaag
     3421 gaggcgcgac gcgctgtcgg cgacgctggc gcgcagggcg gcggcgccgg catgggcgcc
     3481 gccggcgggc aggacgaatt gccggttctc cttggtctct tcattccaga gggaggagat
     3541 ggcggccccg aggaggtccc gggggaccca gcccgcgcgg tcaccttcga gttcgatgta
     3601 gctctcgagg aacgcgtcga agattgcccg ggcgcgtcca tcctcccccg tcctctgcag
     3661 gcattccctg agggcgaggt cgatgagctc ctttgtttcc gttgacacct cgaagtccgg
     3721 cggcaggttg agctgcatcg ccgaggcctt gagcgtgagg tttacgaaac tgtcgatggt
     3781 gctgacgttg aagcggccga aatccttgag cagctccgtg aaccgtcggt ccatggcgcc
     3841 catgagatag tccctgacct tcagggccct ggcggtaagg tcgccggctt cctgaagggc
     3901 gtccgaggcc ccccggtccg gcagaggggc gtcgggcagg actgtcgtta tccccttcat
     3961 gatagtatcg aggggtttga gtgatgagtt ctcgaagggc aggtcgagga ttattcgctt
     4021 catccattcc aggatgcggg tgcgcatctc ctgcgccgcc ttgttggtga aggtgatggc
     4081 gacgatgttc gccatgcggg tggggagggt ggtgtccgcc agcgcggcgg caaggaggac
     4141 cttcagatag tgctcggcga ggcggtatgt cttgccggag ccggctgagg accggacggt
     4201 gatgatctgg ggaacagttc cccttgtcct gtcgtcggcc atggacgacg tcagcggtaa
     4261 tcctgttctt gagtgattgc ggcgagcttc cagtcgcgct cgccggtctt gcgcgtatag
     4321 gtccagtatt cgaggaactt caccgggtcc gtggggctgc ccgacaggac ggtgcccgtc
     4381 tcgtcggtgg tgaagtcgag aaggcttgcc gtgtaccgga cggtgacgaa ctcctcgccc
     4441 tggtcctgga cggcgtcgac gatctccacc tgccgcaccg ctatgttctc caggcggttt
     4501 atcttctttt cagcgaggta gcggctgata tctccctgga agatgtcgcg tgtctgaggc
     4561 gtgagaaggc cggcgatgcc gccgatgtcg cgccgcgtcc agccgccctg tatcttgaag
     4621 aaaaggtcct cggcggtctc gcggaaggac tgctcgctga atgccgggtc cagtgcc
//
LOCUS       DAOQTR010000101         4676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20439_length_4676_cov_6.020126, whole genome shotgun sequence.
ACCESSION   DAOQTR010000101 DAOQTR010000000
VERSION     DAOQTR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20439_length_4676_cov_6.020126"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKN85_03550"
     CDS             complement(<1..442)
                     /locus_tag="PKN85_03550"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNT43509.1"
                     /translation="MNIILFAVVIVLAAFAVAPLFNARVKFTLPAGKTRAEAKQESPA
                     AQSVSAPSPSDYAAIAENNLFHPERRTPKEEKPLPKPELVLYGTIVAEGGSVAYIEDK
                     KSPKATPGRGKRQTAVKKGDVISGFVLKEIEVDRIVLTRGEETMV"
     gene            complement(483..1037)
                     /gene="gspM"
                     /locus_tag="PKN85_03555"
     CDS             complement(483..1037)
                     /gene="gspM"
                     /locus_tag="PKN85_03555"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNT43510.1"
                     /translation="MEQRSKLLKFAIPVIVVLFVLVAYQYGYERIRAEMSSVREMQAM
                     KFRTLEKYITVVAERPLIEARLASSKEQRKADDAKIIEGQTYSLSANTLEDIVRGIIT
                     GRGGSISSERVEKPDDLGAFKVVNVSMDVMLPDTRALSDVIYSIETRTPYIVVRELDV
                     RARNIREPRELTVKLRIAALTGGK"
     gene            complement(1021..2556)
                     /locus_tag="PKN85_03560"
     CDS             complement(1021..2556)
                     /locus_tag="PKN85_03560"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNT43511.1"
                     /translation="MISGSAIERLSASGAEFAAKAGKLRAPVWNALTFPLMEGMIVPK
                     KAVSASIEKGALWVVYGSRFLSKIMVKDFRRYAFEEGKYPGPDGLASTVSIALRELKA
                     EGAGIILCIPGDWVIMRTAQLPSTVKENIADVISYELDRLTPFSSENAYYDFRVMGES
                     SGMLSVVIAAARADLVNQYLDALREKSIEVEKVTINLSNTGTLCSYVSQEKNPVFLNA
                     GPLEYEGGVLRDNILLSSFGDSLDGKDGRSKIEAMAAVLEPVVAAAAEQNGSPPAIVC
                     LGNGNCGELGDHLDAPVKVLSDNDIRQKLLADKEGISSAAAGGALESLWSGARGFNLL
                     VKGAYDRARPSMAVTLALIILLLVALVPYVLLPLQKEERRLAEIDRQISLRKEEVMKV
                     EGLRKEADVLMNEMAAIRAFKEARPMALVILRELTGILPKTAWLTRIKMTETTVNIEG
                     YAASASEILPKLEQSQYFEKVEFSSPTVRDTRLNADRFVMKMEIEGSVKKGEEKEKDG
                     AKK"
     gene            complement(2562..3524)
                     /locus_tag="PKN85_03565"
     CDS             complement(2562..3524)
                     /locus_tag="PKN85_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNT43512.1"
                     /translation="MMRNEKGFALLMVLWIMMILMVISLSFSLMTRTETHATLYFKEG
                     TENRFLAEAGVQRAVMELLHRGVYKNKQPVLEGNEPVKVDGTRYTGQLGDDSYVFDIF
                     DESGKISLNSLTDKSGVIMSNLLVNLGARREDADAIVDAILDWKDGDEVRRLNGAESD
                     YYMSLPNPYRSKNGAFDTVEELLMVKGMTPEILYGNSRQPGIFDFLTVRARGGGINVN
                     SASREVLAALPGMTDEAVDRIIELRGLAEIKSQEEIKPAVGGGVALMSPYIGFSEANT
                     YTVTATGYKKGEKHGFTVKAGITVESNNKYRYTYYKSPAGSSRR"
     gene            complement(3527..4117)
                     /locus_tag="PKN85_03570"
     CDS             complement(3527..4117)
                     /locus_tag="PKN85_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43513.1"
                     /translation="MLRSERGLTLLELLISIVLMVTILLIVSGAMRLGYRSVDRGEKK
                     VEHLERYRSSLAIIHAQVQSGMPLVASREGVAKRYYFAGAADSLRIATNYSIWGGQKG
                     HVIVEYRIEADGNGVRSLIASENMVGAEKKNEALLLQGFDAIFFEYFYKGAAEQEGRW
                     VDGWSDNAKLPGKIRLRLVQGRKEVSMIIPMRAHGI"
     gene            complement(4120..4563)
                     /locus_tag="PKN85_03575"
     CDS             complement(4120..4563)
                     /locus_tag="PKN85_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT43514.1"
                     /translation="MKGEKLKTSHDFRFTDHELRITDCAGFTLLEVLVALAILGTAVT
                     LIFQLFSANMRNIAISEDYLSATVAAEARMREVLDDDALTENSWSETTGDGYRIDVVV
                     SDALQQRTGELPVKLLEVAMTVSWAKNMKQKSLTLRTMKMVQRKI"
BASE COUNT          904 a         1484 c         1158 g         1130 t
ORIGIN      
        1 ccaccatcgt ctcctcgcct cttgtgagca ctatcctgtc gacctcgatc tccttcagaa
       61 caaaaccgct gatgacgtcc cccttcttta cggccgtctg ccgctttccc cttcccgggg
      121 tggccttcgg ggacttcttg tcctctatat aggcaacgct gcccccctcg gccacgatgg
      181 tcccgtagag cacaagctcc ggcttaggca gtggcttctc ctccttcggc gtccttctct
      241 cgggatggaa taggttgttc tccgctatcg ccgcataatc ggaaggggac ggcgccgaca
      301 cgctctgtgc ggcgggagat tcctgcttcg cctcggccct tgtctttccc gcaggaaggg
      361 tgaactttac gcgcgcattg aagagggggg ccacggcaaa ggcagcgagc actatgacca
      421 ccgcaaaaag gatgatgttc aaaagattga tttcgctgta aaaacgttga agagaacgca
      481 tcctattttc cacctgtcag cgcggcaatc ctcagcttca ccgtgagctc tctgggctcc
      541 ctgatattcc tcgccctcac gtcaagctct ctgacgacga tatagggcgt ccgcgtttct
      601 atgctgtaga taacgtcgct cagcgcccgc gtatcgggca acattacgtc catgctcaca
      661 ttaacgacct tgaaggcgcc gaggtcgtcc ggcttctcga cgcgctcgct cgatatggag
      721 ccgcccctgc ctgtgatgat gcctctcacg atatcttcga gggtattggc ggaaagcgag
      781 tacgtctgcc cctctatgat cttcgcgtcg tcggccttcc tctgctcctt tgaggaggca
      841 agcctggcct caatgagggg cctctccgcg acgacggtta tgtatttttc aagggtcctg
      901 aacttcatcg cctgcatctc cctgaccgag gacatctccg ccctgatcct ctcataaccg
      961 tactggtacg ccacaaggac gaaaagaacg acgatcacgg gaattgcaaa ttttagcagc
     1021 ttacttcttt gctccatcct tttccttttc ttcccccttt ttcaccgagc cctcgatctc
     1081 catcttcatt acaaacctgt cggcgttaag cctcgtgtcc cgaacggtag gcgatgaaaa
     1141 ctcaaccttc tcgaagtatt gcgactgctc aagcttcggc agtatctccg aggcggatgc
     1201 cgcgtaccct tcaatattaa cggtcgtctc cgtcatctta attctcgtaa gccacgcggt
     1261 cttcggaagg atcccggtaa gctccctgag gatgacaagc gccatgggcc ttgcttcctt
     1321 gaaggccctg atggccgcca tctcgttcat caggacatcc gcttcttttc tcaacccttc
     1381 gaccttcatc acctcttcct tccggagcga tatctggcgg tcgatctccg cgagcctcct
     1441 ctcctccttc tggaggggta gcagaacata gggtacgagg gcgacgagca gaagaatgat
     1501 caaggcaagg gtaactgcca tggacggccg cgccctgtcg tatgcccctt tcacaagcag
     1561 gttgaacccc cttgccccgg accacaggga ctcgagtgcc ccgcccgccg cggcgcttga
     1621 tatgccttcc ttatcggcaa ggagtttctg tctgatgtca ttatcgctca atacctttac
     1681 aggcgcatcg aggtgatctc ccagctcgcc gcaatttcca ttccccaggc agacaatggc
     1741 cggcggtgag ccgttctgct ccgcggcagc ggcgacaacc ggctccagca cggctgccat
     1801 tgcttcaatc ttcgaccttc cgtcctttcc gtccaagctg tcgccgaaag aggacaggag
     1861 gatgttgtcg cggagtaccc cgccttcata ttcaagcggg ccggcattca gaaagacagg
     1921 gtttttctcc tggcttacat agctgcagag cgtgccggta ttcgaaaggt tgatcgtgac
     1981 cttctccacc tcgatggact tttcccgcag cgcgtccagg tactggttta caaggtcggc
     2041 cctggcggca gctatcacga cgctcaacat cccgctgctc tcacccatga ccctgaaatc
     2101 gtagtatgca ttctctgagc tgaaaggcgt cagcctgtcc agctcatagg agatcacgtc
     2161 cgcaatgttc tccttcaccg tggagggcaa ctgcgccgtc ctcatgatga cccagtctcc
     2221 ggggatgcac aggatgatcc ctgcgccttc cgccttgagc tccctcagcg cgatggaaac
     2281 ggtcgaggca aggccatcgg ggcccggata tttcccttcc tcaaaggcgt acctcctgaa
     2341 atcttttacc atgatcttcg acaggaaacg cgacccgtac accacccaga gggcaccttt
     2401 ctcgatagag gccgagacgg cctttttggg gacgatcatg ccctccatga gcgggaatgt
     2461 aagggcgttc cagacaggcg cccggagctt cccggccttc gcggcaaatt ctgcgccgga
     2521 ggcggatagt ctttctatgg ccgatcctga tatcattttc gtcatcgccg gcttgagcct
     2581 gccgggctct tgtaatatgt gtaccggtat ttgttgttac tttccacagt tattcctgct
     2641 ttgacggtaa aaccatgctt ctcgcccttc ttgtatcccg tcgccgtgac cgtgtatgtg
     2701 ttggcctctg aaaagccaat gtacggtgac atcagggcaa cgccgccgcc gacagcgggc
     2761 ttgatctcct cctggctctt tatctccgcc agccccctga gctcgatgat ccggtcaaca
     2821 gcctcgtccg tcatgcccgg cagggctgca agcacctccc tggaggccga attcacattg
     2881 atcccccctc cccttgcccg gaccgtaagg aaatcaaaga tgcccggctg gcggctgttt
     2941 ccatagagga tctcaggcgt catccctttt accatcagga gctcttcgac ggtatcgaac
     3001 gcgccgttct tcgacctgta aggattaggg agcgacatgt agtagtcgct ctcagcgccg
     3061 ttcagcctgc ggacctcgtc gccgtccttc cagtccagga tcgcatccac gatggcatcg
     3121 gcatcttccc tcctggcccc gaggttcacg aggagattgc tcatgatcac gccggatttg
     3181 tctgtcaacg agttcaggct tatctttccc gactcatcga aaatgtcgaa gacataggag
     3241 tcatctccga gctggccggt atatctggtc ccatcgacct tgaccggctc attcccttct
     3301 aaaaccggct gcttgttctt atagacgccc ctgtgaagca actccataac ggcgcgctgc
     3361 accccggcct ccgcaaggaa cctattctcc gtcccttctt taaaatagag ggtagcatgg
     3421 gtctccgtcc tggtcatcag ggaaaaagag agggagatga ccatgaggat catcatgatc
     3481 cacagcacca tcaaaagggc aaaacctttt tcgtttcgca tcatcctcag atcccgtggg
     3541 ccctcatggg gatgatcatg gagacctctt ttctgccctg tacgagacga agccgtatct
     3601 tgccgggcaa ttttgcgttg tcgctccacc catcgaccca tcttccttcc tgctcggcag
     3661 cacccttata aaaatattca aagaatattg catcaaaacc ctgaagaagc aaggcctcgt
     3721 tcttcttctc tgcgcctacc atgttctcgc ttgcaataag gctcctgacg ccgttcccgt
     3781 ccgcttcgat cctgtattca acgatcacgt ggcctttctg cccgccccag atggagtagt
     3841 tcgtcgctat cctcagcgag tctgcggcgc cggcgaaata ataccgcttg gccaccccct
     3901 cccgtgaggc taccaggggc atccccgact gcacctgcgc gtggatgatc gccagagacg
     3961 accggtacct ctcgaggtgt tcgacctttt tctctccgcg gtccaccgag cggtacccga
     4021 gcctcatggc gccggagaca atgaggagga tcgtcaccat caggacgatg gagatcaaaa
     4081 gctccagcaa ggtaaggccg cgttcgcttc tcagcatctt cagatcttcc tctggaccat
     4141 cttcatggtc cttagcgtaa ggctcttctg cttcatgttc tttgcccagg agaccgtcat
     4201 ggcgacctcc aggagcttta ccgggagctc ccctgtcctc tgctgcagcg catcggacac
     4261 gaccacgtct atcctgtagc cgtcaccggt cgtctcgctc cagctgtttt ctgtcagcgc
     4321 gtcgtcgtcg agcacctcgc gcatccttgc ctctgccgcc accgtggcgg agagataatc
     4381 ctcggaaatg gcgatgttcc tcatgttggc agagaagagc tggaagatca gcgtcacggc
     4441 cgtgccaagg attgcaagcg ccacaaggac ctcgaggagg gtgaaacccg cacaatccgt
     4501 aatccgtaat tcgtgatccg tgaatcgaaa atcgtgagag gtttttaact tttcaccttt
     4561 cacctttcac ctttcacctt ttaccttgtt cattgtctcg ttaccaccga cccgaccacg
     4621 gggtctgtct ggatatagat cgcctttttc ctgccctgca ggatcaccgt cccgcc
//
LOCUS       DAOQTR010000102         4672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20465_length_4672_cov_3.718648, whole genome shotgun sequence.
ACCESSION   DAOQTR010000102 DAOQTR010000000
VERSION     DAOQTR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20465_length_4672_cov_3.718648"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKN85_03580"
     CDS             <1..369
                     /locus_tag="PKN85_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNT43515.1"
                     /translation="AALLASSEYIIHRAIYLATGAGAIVHAHPPYATLLSMSGELVPI
                     DVEGGYHLGRVPVATPRNPTASEEAAHAASELMRTNRVILIRAHGSFSRGETLEEAYM
                     LTSTLEASAFYLWNIDKMRA"
     gene            complement(527..2200)
                     /gene="ggt"
                     /locus_tag="PKN85_03585"
     CDS             complement(527..2200)
                     /gene="ggt"
                     /locus_tag="PKN85_03585"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNT43516.1"
                     /translation="MSVLVPVSDVSAEETAIAFPVEGKRAMVVTGQELATKEALLVLK
                     KGGNAVDAAVTAAFVMAVTLPRAGNIGGGGFMLIHNAATKEVIALDFWQKAPAAASRT
                     MFVRDGKADAKLSRESYIAVAVPGTVAGLAFALGNYGTISLGEALRPAIRYAEKGFVM
                     GAGLSSDIKSYEKLLKARPGSARKFFKPSGGYYEAGDLFVQKDLARTLEALAKGGPDV
                     FYKGDIAARIAKQMKENSGLVTKEDLAAYSPVARRPVQTTYRGHDVYSMYPPSSGGVC
                     IAEALGILERFDLARAGHNSAAGIHLIAEASKRAFADLFVYAGDPDAVAIPVKGLMSK
                     AYGAKLAAGIDPARATPSSQIRSGDAKVYEKDETTHFSIVDAWGNAVSATYTLNGNFG
                     SGIVVDGAGFLLNNEMDNFNSNPGTPDGAGIIESEANAIAPNKRMITAMAPTMVFRQG
                     KLFLVTGSPGSSRIISTVLQVILNVIDHKMNIQEAVNAPKVHHEWMPDELRVEKGISP
                     DTMKLLEQMGHNVVLRGPMGSATSILIDPATGKRYGAVDPRREGLAEGY"
     gene            complement(2458..3573)
                     /locus_tag="PKN85_03590"
     CDS             complement(2458..3573)
                     /locus_tag="PKN85_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNT43517.1"
                     /translation="MDIINEYLKYMVEKDASDIYFTVGVPPAFRIEGVVSLYGDKALT
                     AEDTKSIAYAIMNERQQAKFEEEMEMNLAIFQPEYGRFRVNIFRQKSFIGIVIRQIKL
                     TIKTIDDWGLPAIMKDICMSKRGLVLVVGATGSGKSTTLAALIDYRNSNQPGHIITIE
                     DPVEFVHQHKMSVVTQREVGFDTLSFYNALKNTLRQAPDVILIGEIRDAETMEDAITF
                     AETGHLCLATLHANNANQALERILNFFPIERHLQIYMQLSLNIRSIVSQRLIPTVEGK
                     RAAAIEILLDSPRVKDLILKGEITLLKETMAESYFEGMQTFDQHIFDMYQSGLLDLNN
                     AIAYADSPNDVRLKIKMAEMKPDDAAEKKDSGLKLKL"
     gene            complement(3577..4611)
                     /locus_tag="PKN85_03595"
     CDS             complement(3577..4611)
                     /locus_tag="PKN85_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNT43518.1"
                     /translation="MDISELLRFALESGGSDLHISAGEPPVIRIHGEMTKVDLPPLDK
                     EDVHNMIYDILSDFQRKVFEEHLELDFSFGLGDLARFRVNVFKQQRGESAVFRTIPSK
                     IPTFDELNLPRVFKDIAGLEKGLVLVTGPTGSGKSTTLAAIVDFINDTVKEHILTIED
                     PIEFLHNSKKCLVNQRELGPHTKSFANALRSALREDPDVILVGEMRDLETIQLALTAA
                     ETGHLVFATLHTSSAPDTVDRVIDVFPAGQQNQVRSMLSGSLQAVISQALFKRRDGKG
                     RVAGFEVMIATPAVRNLIRENKIAQIPSMIQTSKGIGMQTMEAACNELIAKNLVTRDE
                     VSFYLSSSMR"
BASE COUNT          835 a         1415 c         1383 g         1039 t
ORIGIN      
        1 gccgccctgc tcgcctcgtc ggaatacatc atccaccgcg ccatctacct cgccaccggc
       61 gccggagcca tcgtccatgc ccacccgcct tacgcgaccc ttctttccat gagcggagag
      121 ctcgtcccca ttgacgtcga agggggctat cacctgggac gcgtccccgt cgccaccccc
      181 cgcaacccca ccgcctccga agaggcagcc cacgcggcga gcgaactcat gagaacgaac
      241 agggtcatcc tcatcagggc ccacggcagc ttctcccgcg gcgaaaccct cgaagaagcc
      301 tacatgctca cgagcaccct cgaagcctcc gcgttttatc tgtggaacat agacaaaatg
      361 cgggcttgaa tcgcttgaat cgcttgaggg ttaagaacat cacgggaccg tacggcccgt
      421 ccacccggcg gaagccggga ccggaacttt ccccgatccg gctgcttctc atcttctgcg
      481 tctcactctc aagccattca agcgattcaa gccattcaag ccgtcttcaa tacccctctg
      541 ccagcccttc cctcctcggg tccaccgcgc cgtatctctt cccggtggcc gggtcgatga
      601 ggatgcttgt cgccgagccc atggggcctc tcaggaccac gttgtggccc atctgctcca
      661 gtagtttcat cgtgtcgggg cttatgccct tctcgacgcg cagctcgtcg ggcatccatt
      721 catgatggac cttcggggcg ttgaccgcct cctggatgtt catcttgtgg tcgatgacgt
      781 taaggatgac ctggaggacc gtcgagatga tgcgggaact gccgggactg cccgtcacga
      841 gaaaaagctt cccctgccgg aagaccatcg tgggggccat ggcggttatc atcctcttgt
      901 tgggggcgat ggcgtttgct tcgctctcga tgatgccggc accgtcgggc gtgccggggt
      961 tggagttgaa gttgtccatc tcgttgttga ggagaaaccc cgcgccgtcg accacgatgc
     1021 cgctgccaaa gttcccgttg agggtatacg tggcggacac cgcgttgccc caggcgtcga
     1081 cgatggaaaa gtgcgtcgtc tcgtccttct cgtagacctt agcgtctccc gaacgtatct
     1141 ggctgctcgg ggtcgcccgg gcgggatcga tgcccgccgc cagcttcgcc ccgtaggcct
     1201 tcgacatcaa ccccttgacg gggatggcaa cggcgtcggg gtctccggcg tagacgaaaa
     1261 ggtcggcgaa ggcgcgcttc gaggcctcag cgataaggtg gatgccagcg gcactgttgt
     1321 gcccggcacg ggcaagatcg aatctctcga gaatgccgag ggcctcagcg atgcagaccc
     1381 cgcccgaact gggcgggtac atcgagtaca cgtcatggcc ccggtaggtc gtttgaacgg
     1441 gcctgcgggc caccggtgag taggcggcaa ggtcttcctt cgtcacgagg cccgagttct
     1501 ccttcatctg cttcgctatc ctcgcggcaa tgtctccctt gtagaaaaca tcaggccccc
     1561 ctttcgccag ggcttccaag gtccgggcaa ggtccttctg gacaaagagg tcgccagcct
     1621 cgtagtaccc gcccgacggc ttgaagaact tcctggcgga ccctgggcgg gccttgagca
     1681 atttctcgta ggacttgatg tccgaggaga ggccggcgcc catcacaaac cctttctccg
     1741 catacctgat ggccggcctg agggcctcgc cgagggatat ggtcccgtag tttccgagtg
     1801 cgaaggcgag acccgccacg gttcccggca ccgccaccgc gatgtaactt tcccggctca
     1861 gtttggcgtc cgcctttcca tcccttacga acatggtgcg cgacgccgcc gcgggggcct
     1921 tctgccagaa atcgagggcg atgacctcct tcgtcgcggc attgtggatg agcatgaacc
     1981 cgcctccgcc gatattgcct gccctgggca gggtcacagc catgacgaag gcggcggtca
     2041 cggccgcgtc gacggcattc ccacccttct tgaggacgag gagcgcctcc ttcgtcgcca
     2101 gctcctgccc cgtcacgacc atcgctctct tcccctcgac gggaaaagcg atcgccgtct
     2161 cttcagcgga aacatcagaa actggaacca gaacagacat caaagatacc gcaagcagca
     2221 acaatgcagc gccaataccc gttttcctca tagtcatcat cctcggtttc tctgaaggcg
     2281 gcttgaatgg cttgagtcgc ttaacagact gttgacaaac tcctcttttc gtcatcccgg
     2341 acttgatccg ggatccaaag gtttttctaa cccattgtgt tttctggatt ccggccttcg
     2401 ccggaatgac ggtgataata aacggttcta acggctcaag cgattcaaga gccgcctcta
     2461 gagcttcagc ttcagtcccg aatccttctt ctccgccgcg tcgtcgggct tcatctcggc
     2521 catctttatc ttgagccgca cgtcgttggg gctgtccgcg taggcgatgg cgttgttgag
     2581 gtccagaagg cccgactggt acatgtcgaa gatgtgctga tcgaaggtct gcatcccttc
     2641 gaaataggac tccgccatgg tctctttcag gagggtgatc tcgcccttga ggatgaggtc
     2701 cttgaccctc gggctgtcga gaaggatctc gatcgctgcc gcgcgcttgc cctccactgt
     2761 ggggatgagc ctctgggata cgatggaacg gatgttgagg gaaagctgca tgtagatctg
     2821 gaggtggcgt tcgatgggaa agaaattgag tatcctttcg agggcctggt tcgcgttgtt
     2881 cgcgtggaga gtggcgaggc aaaggtggcc cgtctccgca aaggttatgg catcctccat
     2941 cgtctccgca tccctgatct caccgatgag gatgacgtcg ggcgcctgcc gcagggtgtt
     3001 cttgagggcg ttgtagaaac tcagggtgtc gaagccgacc tccctctgcg tgacgacgct
     3061 cattttgtgc tggtggacga actccaccgg gtcctcgatg gtgatgatgt ggccgggctg
     3121 attggaattg cggtagtcga tgagagcggc cagggtggtc gacttgccgc ttcccgtggc
     3181 gccgacgacg aggacgaggc cgcgcttgga catgcatatg tccttcatga ttgcgggcag
     3241 cccccaatcg tcgatggtct tgatcgtgag cttgatctgg cgtatgacga tgccgataaa
     3301 gctcttctgc cggaagatgt tcacccggaa cctgccgtat tcgggctgga agatggccag
     3361 gttcatctcc atctcctcct cgaacttcgc ctgctgccgc tcattcatga tggcgtaagc
     3421 gatgctcttc gtgtcctccg cggtgagagc cttgtcgccg taaagggata cgacgccttc
     3481 gatgcggaaa gccggtggca cccccacagt gaagtagatg tccgacgcgt ccttttccac
     3541 catgtacttc aggtactcat tgatgatgtc catggatcac ctcattgagc tgctcaggta
     3601 gaaggaaacc tcgtccctcg tcacgaggtt cttggcgatg agttcgttgc aggccgcttc
     3661 catggtctgc atcccgatcc ccttgctcgt ctggatcatg gagggtatct gagcgatctt
     3721 gttctccctg atgaggtttc tcacggccgg cgtggcgatc atgacctcga agccggcgac
     3781 cctccccttg ccgtcacgcc tcttgaaaag ggcctgggaa ataacggcct gcagggagcc
     3841 ggagagcatg gagcgcacct ggttctgctg gcccgcgggg aagacgtcga tgaccctgtc
     3901 gaccgtgtcg ggagccgaac tggtatggag ggtggcgaat acgaggtgtc ccgtttcagc
     3961 cgccgtcagg gcaagctgga tggtctcgag gtccctcatt tcgccgacga gtatgacgtc
     4021 ggggtcctca cgcagggcgc tcctcagggc gttggcgaaa cttttcgtat gcggtcccag
     4081 ctcacgctgg ttgacgaggc atttcttgga gttgtggagg aactcgatgg ggtcctcaat
     4141 ggtgaggata tgttccttca cggtatcgtt gatgaagtcc actatcgcgg cgagcgtcgt
     4201 cgacttgccg ctgccggtgg gccccgtgac gaggacgagg cccttttcca ggcccgctat
     4261 gtccttgaat acgcggggca ggttgagttc atcgaaggtg ggtatcttgc tcggaatggt
     4321 cctgaagaca gccgattcgc cgcgttgctg cttgaagacg ttgaccctga agcgggccag
     4381 gtcgccaagg ccgaaggaga agtcgagctc gaggtgctcc tcgaagacct tccgttggaa
     4441 gtcgctcagt atgtcataga tcatattgtg cacgtcttcc ttatcgaggg gcggcaagtc
     4501 gaccttcgtc atttcgccgt gtatcctgat cacggggggt tccccggcgc tgatgtgcag
     4561 gtccgatcct ccgctttcga gggcgaaacg cagcaattca gatatgtcca tggtgtcctc
     4621 ctatccttcg agcgattcta tctccttata tgaattgctg cgtttcgccc tc
//
LOCUS       DAOQTR010000103         4668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20490_length_4668_cov_3.968567, whole genome shotgun sequence.
ACCESSION   DAOQTR010000103 DAOQTR010000000
VERSION     DAOQTR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20490_length_4668_cov_3.968567"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKN85_03600"
     CDS             <1..612
                     /locus_tag="PKN85_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43519.1"
                     /translation="KESRKIFQRMNSYAIYRITETIRVLLFMTLAILVFNFYPVTAVM
                     IVLLALLNDGAILSIAYDNVRYADRPEAWNMPAVLSIATVLGFAGVVATFGLFYLGER
                     VFHLSRDVIQTLMYLKLSVAGHMTIFITRTRGPLWSHRPANILLVAVIGTQLVATIFA
                     VYGILMTPIGWGWALAVWGYAFAWFLVNDRIKVATYRVIGTPD"
     gene            complement(664..1185)
                     /locus_tag="PKN85_03605"
     CDS             complement(664..1185)
                     /locus_tag="PKN85_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNT43520.1"
                     /translation="MILIYTAGQRKLYRNVRPLKLDVFLYSYNEVYQVVNRIKGTALF
                     FARTTKKIPAYIVHTMFMNNIVYEENILVSITTLDEPFGLTSFFKDDLAPGLRSFEIQ
                     MGYMEVVDIGQILKEAGITGEKGIFYGLEEIYTRNVLWKLFSIMKKLAPPFVQFHDLP
                     SNKLHGVTTRIEI"
     gene            complement(1070..2524)
                     /locus_tag="PKN85_03610"
     CDS             complement(1070..2524)
                     /locus_tag="PKN85_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNT43521.1"
                     /translation="MQNQAIHPRAGENRHRAVDDIRSVIKSLGLVFGDIGTSPIYTLT
                     VIFLLLKPTVDNVMGVLSLVVWTLIVLVSVEYAWLAMSLGEKGEGGTIILRGHLIRLL
                     KRGRGAVFVTILAFIGISLLVGDGVITPAISILSAVEGILLIPGLHNTERTTLILIAG
                     AIAIALFAFQRKGTGKVAGAFGPLMLLWFLVLAVSGVASIIRFPSVWKAINPWHAVTF
                     LYENGAAGFFVLSEVILCATGAETLYADMGHLGRKPIIQAWCFVFLALVLNYLGQGAF
                     ILSNPAESRNVLFGMAFYQSSLLYIPFLVLSILATIIASQAIISGMFSIVYQGINTRL
                     MPMFRVEYTSPELRSQIYIGFVNWFLLLSVLFIIFQFGESKELAAAYGLAVTGDMTIT
                     GILITSIFWLRKDYWKSLIAFLVTIVDVVFLTSNIYKIPHGGVLVARYCRRSPRHDPH
                     LHGRSEEALPQCPSLEAGCLPLQLQRGLSGRQQD"
     gene            complement(3125..3607)
                     /gene="tsaA"
                     /locus_tag="PKN85_03615"
     CDS             complement(3125..3607)
                     /gene="tsaA"
                     /locus_tag="PKN85_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNT43522.1"
                     /translation="MKDEGLQFIVRPIGVIHSPFRTKEETPIQGRFRPDAVGSIEIYE
                     EYREGLKDIEGFSHIFLIYPFDRAGRVELVRPVLLDDEPHGIFACRYPARPNCLGLTI
                     VELIGRNGSTLEVAGIDVLDGTPLVDIKPYVARFDCFPHAKEGWFKGKENRPKPKGRE
                     "
     gene            complement(3711..>4668)
                     /locus_tag="PKN85_03620"
     CDS             complement(3711..>4668)
                     /locus_tag="PKN85_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNT43523.1"
                     /translation="ARNVLFDKTGTLTEGVIVLADVIPLASSDEKEVLWIVFNLEKQS
                     EHPFSDALRKKAQESGIPSLDVTDFEAVIGRGIKGRIGGADYRMGNRTFYEEEGHILD
                     NRTRDLYEEKERSAASPVLLWSETGLMAILTFSDRVREESIEVVRKLREMNVEPVMIT
                     GDSENGARTISGRLGIERYFHRVLPDGKAAIVEEFRKKGVTVMVGDGINDAPSLAAAD
                     IGVAMGKGTDIAIESADVVLMKGHLSRLVSLIRLSRKTMRVIKQNLFWAFIYNVLGIP
                     IAFGALYPFFGIRLEPVFGAVAMVISSISVVSNSLRLKFFKD"
BASE COUNT          984 a         1310 c         1331 g         1043 t
ORIGIN      
        1 aaggaaagcc gcaagatatt ccagagaatg aacagctacg cgatataccg gataacggaa
       61 accatccggg tcctgctgtt catgacctta gccatcctgg tattcaattt ttatcctgtc
      121 accgctgtca tgatagtgct cctcgcgttg ctcaacgacg gtgcgatcct gtccatcgca
      181 tatgacaacg ttcgctatgc ggacaggccg gaggcctgga atatgcccgc ggtattgagt
      241 atagctaccg tgctgggttt tgccggggtg gttgccacct tcggactgtt ctatcttgga
      301 gagcgggtat ttcatctcag ccgggatgtc atccagacgt taatgtatct gaaactgtcc
      361 gtagcggggc atatgacgat attcatcacc cgtacacggg ggcccttgtg gagccaccgt
      421 cctgcaaaca ttctccttgt ggcggtgatc ggcacccagt tggtggcgac gatatttgca
      481 gtctacggga tactcatgac tcccatcggc tggggctggg cgctcgcggt gtgggggtat
      541 gcatttgcct ggttcctggt taacgacagg atcaaagtgg cgacctatcg cgtcatcggg
      601 acgccggact aggtcgccgg cggtagaggg tgtttgcgcc caggaacccc ggatccgcct
      661 ttcctatatc tctatcctgg tggtgacacc gtggagtttg ttggaaggca gatcatggaa
      721 ctggacgaaa ggcggcgcca gctttttcat tatggagaat agcttccaca gcacgtttct
      781 cgtatagatc tcctcgagcc cgtaaaagat ccccttttca ccggttatgc cggcttcttt
      841 cagtatctgc cctatatcga ccacttccat gtatcccatc tgtatctcga aggacctcag
      901 gcccggggcg aggtcgtcct tgaagaagct cgtgagcccg aagggttcat cgagggtcgt
      961 gatggaaacg aggatgtttt cctcgtagac gatattgttc atgaacatgg tatgcacgat
     1021 gtaggcgggg atcttcttcg tggtgcgggc aaagaacaga gccgtgcctt taatcctgtt
     1081 gacgacctga tagacctcgt tgtagctgta aaggaagaca tccagcttca agggacggac
     1141 attgcggtaa agcttcctct gaccggccgt gtagatgagg atcatgacga ggggaacggc
     1201 ggcaataacg agcgaccagt acccccccgt ggggtatctt gtatatgttg gacgtcagga
     1261 agacaacgtc gacgatggtg acgaggaatg ctatcaagga tttccagtaa tcctttctca
     1321 gccagaaaat ggaggtaatg agtatccccg tgatcgtcat gtccccggta acggcaaggc
     1381 cgtacgcggc ggcgagttcc tttgactccc cgaactggaa gataatgaag aggacggaca
     1441 ggagcaggaa ccagttgacg aaccctatgt atatctggga cctcagttcc ggcgatgtgt
     1501 actcgaccct gaacatgggc atgagccgtg tgttgatccc ctgatagacg atggagaaca
     1561 tgccgctgat tatcgcctgg gacgcgatga tggttgccag gatggagagc acgagaaagg
     1621 gtatatagag cagggaggac tggtagaatg ccatgccgaa gaggacgttc cttgactccg
     1681 ccggattgct caagatgaac gcgccctgcc cgaggtagtt gagtacgagg gcgagaaaga
     1741 cgaaacacca cgcctggatg atgggttttc tcccgaggtg gcccatgtcc gcatagagtg
     1801 tctctgcccc agtcgcgcag aggatgacct cggagagcac gaagaagccc gcggcaccgt
     1861 tctcgtacag gaaggtgacg gcatgccagg gattgatggc cttccacacc gaggggaacc
     1921 tgatgatcga ggccacgccg gagacggcca ggaccaggaa ccacaggagc atgaggggcc
     1981 cgaaggctcc cgcgaccttc cccgtgccct tcctctggaa ggcgaagagc gctatggcga
     2041 tcgctccggc gatgaggatg agcgtggtac gctccgtgtt gtgcagtccg gggatgagga
     2101 gaatgccctc cacggcgctg agtatgctta tcgcgggagt gatgacgccg tcgccgacaa
     2161 ggagagatat gccgatgaag gccagaatgg tcacgaagac cgcccctctt cccctcttca
     2221 gaagtcggat caggtggccc ctgagaatga tcgtccctcc ctcgcccttt tcgccgaggc
     2281 tcatggcaag ccacgcgtac tcgacggata ccaggactat cagggtccat accacgaggg
     2341 aaaggacgcc catgacgttg tcgaccgtcg gcttgaggag caagaaaatg acggtcaggg
     2401 tgtagatggg gctggttccg atgtccccga agacaagtcc cagggatttg atcacggacc
     2461 tgatgtcgtc gacagcacgg tgccggttct cgcccgcccg cggatggatg gcctggttct
     2521 gcattggagt gtcttatgtt acccaatatc gacgggaatt tcaatgcgca gaaggtgcgg
     2581 gtacatcaaa acggggttct tcgcctggca tttgcggcac gcacctgacc acagtagaag
     2641 catgcgagct actcgttccc gtcaagatag atgagttcct tcccgcagat aaaacgatca
     2701 ttcttgtgat ccatgtaata tcccttctcc cccggtcatt cagcccctgt cgggacagcg
     2761 gcgagctctc atttgcccga aaggatttcc ttaactctca acaggctctc gacaagcccc
     2821 tctttttgtc atcccgaccg cctttcttcc tcccccgtca gtccggcgaa ggccggaatc
     2881 caggaaagac tggcagtaga tgaccactga atggtgaaag atccgacaat acctcccgca
     2941 ttccactgtt cctgccgtat ccttttgagg gctttctgct tcaacaggac tcttcctgga
     3001 ttccggcctt cgccggactg acggactgga actgtgaagg actgacggac tggaacggaa
     3061 gccggaccgg ggaccgacat tgccaggggc ctgtcaggcg attcaagcgg ctgcggtgac
     3121 gtgttcattc ccttcccttc ggtttcggtc tgttctcctt gcccttgaac catccctcct
     3181 tcgcgtgcgg gaaacaatcg aagcgggcga cgtagggctt gatgtcgaca agcggcgtgc
     3241 cgtcgaggac atctatgccc gcgacctcga gcgtgctgcc gtttcttccg ataagctcca
     3301 cgatggtgag tccgaggcag ttaggccgcg ccggatagcg gcaggcgaat attccgtgtg
     3361 gttcatcatc gagaagaaca ggccggacca gttcgacccg acctgccctg tcaaaggggt
     3421 agatgaggaa tatgtgggag aacccctcga tatccttgag cccttcacgg tattcctcat
     3481 agatctctat ggaacccacc gcgtccggcc ggaacctccc ctgtatcggt gtctcctcct
     3541 tcgttctgaa aggagaatgg ataaccccta tgggcctcac aatgaactgc aatccttcgt
     3601 ctttcatctc tgtcctttcc caaaatgatt gtttcagtcc ctgttccttc cttgtctttg
     3661 gtctctaacg cttaaacggc tcaagcgatt caagcggttc aagccgtctc tcagtcctta
     3721 aagaacttca gcctcaacga attgctcacc accgatatgg aacttatgac catggccacg
     3781 gcgccgaaga cgggctccag ccttatgccg aagaaggggt agagcgcgcc gaaggcgatg
     3841 gggatgccga ggacattgta gatgaacgcc cagaaaaggt tctgcttgat gacgcgcatg
     3901 gttttccggg agagcctgat aaggctgacg agtcgggaca ggtggccttt catgagcacc
     3961 acgtccgccg attcgatggc aatgtccgtt cctttaccca tggcgacgcc gatgtccgcc
     4021 gcggcgagcg atggggcatc gttgatgccg tccccgacca ttacggtcac tcccttcttc
     4081 ctgaactcct cgacgatggc cgccttcccg tcgggaagga cgcgatggaa gtagcgctcg
     4141 atgccgagcc gcccgcttat ggtcctcgcc ccgttctcac tgtcgccggt gatcatgacg
     4201 ggctccacat tcatctccct gagttttcgc accacctcga tagactcttc ccgaaccctg
     4261 tcgctgaagg tgaggatggc catgagcccc gtctcgctcc acaggaggac cggcgaggcg
     4321 gcggaccgct ccttctcctc gtaaaggtcc cgggtcctgt tgtcgagaat gtgcccttcc
     4381 tcctcgtaga aggtcctgtt ccccatccgg taatcggctc ccccgatcct ccctttgata
     4441 cccctgccga tgaccgcctc gaaatcagtt acgtcaaggg agggtatgcc cgattcctgg
     4501 gcctttttcc tcagggcatc ggaaaaggga tgctcggact gtttctccag attaaaaacg
     4561 atccaaagaa cctctttctc gtcggaggac gcaagcggta tgacgtcggc aaggacgatg
     4621 acgccttccg taagggtgcc cgtcttatcg aagaggacgt tcctcgcc
//
LOCUS       DAOQTR010000104         4666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20500_length_4666_cov_3.474301, whole genome shotgun sequence.
ACCESSION   DAOQTR010000104 DAOQTR010000000
VERSION     DAOQTR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20500_length_4666_cov_3.474301"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..697
                     /locus_tag="PKN85_03625"
     CDS             326..697
                     /locus_tag="PKN85_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43524.1"
                     /translation="MIKYILYVLRWTVLAIPGALFFNKVREFLGIENVYLAMIISQAL
                     MGAMVYFVDRLIFTSGAVPVPWEIKPRGVCVDCGHVGKCYRMPGERCCETGPRTEDSS
                     FRCEKCALKRLKETGTRDGSS"
     gene            979..1779
                     /locus_tag="PKN85_03630"
     CDS             979..1779
                     /locus_tag="PKN85_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43525.1"
                     /translation="MADHNSLKTTGILKYLPAVLVVAGQASVILSLRFEEAMPWLLAL
                     GMALAATGLGLRARSVGRRVVSWVIMGIVFPLLVPLVGFAFMVIKGKKGEAVSPSGAK
                     PVSIPAHAATAFILFLLAALFMDSLSLALISFVIAVIWVSTRKEDRYKGRVRLAVIGV
                     YALMVVMVFAMKGINYRVSQANARVIIEACERYKGRTGEYPAGLRDLVPAYLPEVPVA
                     RYTVMSGGFYYHRNLGPSGSDYRLVFVYESPFARSVYSSVDRGWRSID"
     gene            1904..2101
                     /locus_tag="PKN85_03635"
     CDS             1904..2101
                     /locus_tag="PKN85_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43526.1"
                     /translation="MDSTNRRKDQMSSMARRTVPSFGFVCSGASKGVSGAYTAEADGC
                     KGGCGGQCLSGCKDECITVSK"
     gene            2197..3360
                     /locus_tag="PKN85_03640"
     CDS             2197..3360
                     /locus_tag="PKN85_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM peptide maturase, CXXX-repeat target
                     family"
                     /protein_id="HNT43527.1"
                     /translation="MEKTIARAPAVWQESIAKTVTFVVTEDCQLRCRYCYLVGKNSRN
                     RMSFDTAKRSIDYLVENPDLFPERSIVWDFIGGEPFLEIDLIDEICEYIKEKLSREKH
                     PWLDSHRFSLTTNGILYGSEKVQDFIRRNRPDLSIGVTIDGTPRKHNMHRIYPSGKGS
                     YDDTARNIPLWLEQFPVAATKVTVASDDLPFIRESVLHLYGLGIKHVSINVVFEDVWK
                     DGDDAILEDELVGLADAIIDGEFFTDHTCSFFSETIGRPRTDNNNWCGAGRMLAIDPR
                     GDFYPCHRFTPFSLVNRKFPPVGNCFEGLDRNRLRPFLALDLVSQSRPECIDCEVATG
                     CAWCQGANYDCAETGTIYQRATHLCRMHKARVRANNYYWHRLEGARRPTREAQ"
     gene            3357..4487
                     /locus_tag="PKN85_03645"
     CDS             3357..4487
                     /locus_tag="PKN85_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXXX repeat peptide maturase"
                     /protein_id="HNT43528.1"
                     /translation="MIHYLIVILEHDSAPFCCYDVKERGGPGLIPLDVLEKSVSFAIK
                     NAIPVNFLCGNTALPPGYEELIASVDHVRMVPLARARSGEEAVFIIDGEEDLAHAHVL
                     ETGGLNNVILRVGKSEVPSLPSTVERLLGRFRRLNVILKEPGGFAEEDFSAYADALED
                     VRRILAGEYRKGSFLEISTLSDRVFLTNMNNCNAGTAHLTVAPNGRFYLCPAFYYENG
                     EDCLAGPDDAVEIRNARLLTLENAPVCRNCDAWHCKRCVWLNRRLTSELNTPSHEQCV
                     LAHLEREASRRFLEEIGRECGLNDRVTPIPKLDYLDPFDIISDRSRSPGEKEEHFARL
                     LSRPLEDVPVSDLLRQIYTLDPGMLARLKELNSAVVDLEDGQ"
BASE COUNT          972 a         1390 c         1377 g          927 t
ORIGIN      
        1 ccttgagggt gtatatgctg cagggcgcgc agcagatgtg gagaaatatg tgctttcctt
       61 cgagatccat tggtcggtct tcccaattgc ccgtaatttc ttataattta ccaccttccg
      121 tccctatttt ccacatcggc ggagaaagat cgctgcctcc gggggtccat cttccgggcc
      181 aggacaggga aagcgccttc ctcccccggc atcccgggcg caggacccga aacggtcgtt
      241 gccgccggcc acctcttcct gttgcatatc ggccctgcct gtctcataat aatgatgaca
      301 tagtcgcttg cggcggggag gcagtatgat caagtatatc ctctacgtcc tgcgatggac
      361 cgttcttgcc atcccgggcg ctcttttttt caacaaggtc agggaattcc tgggcatcga
      421 aaacgtttat ctcgcgatga taatcagcca ggccctgatg ggggcgatgg tctacttcgt
      481 ggacaggctc atcttcacat cgggcgccgt accggtcccc tgggagataa agccgcgggg
      541 cgtctgcgtg gactgtggac acgtgggtaa atgctaccgg atgccggggg aaaggtgttg
      601 cgagacgggc ccgaggacgg aggatagttc tttcaggtgc gaaaaatgtg ccctgaagag
      661 actcaaagag acagggaccc gggacggaag cagctgaccc ccgcgtgccg tcatagccgg
      721 gtctgacgga ccttcacgcc acagacggaa cgattcgctc tcccgttacc atccccttct
      781 ggatctatat gcattatcga catcgcgagg aaggatctcg cggacaacaa taagatgata
      841 tggcttcttg ttgttgtctt gttatatact ctgggggtgg cgctttacgt cgccatcggc
      901 aggaagcaaa gggtcggaag gacgggttga gcaccatccg tcgcgagaca aggtcttcaa
      961 ggaaacccgg gtgtacccat ggcagatcat aattccctca agaccacagg cattctgaaa
     1021 tacctccccg ccgtcctcgt cgtggcgggt caggcttcgg tcatactttc cctgcgattc
     1081 gaagaggcaa tgccgtggct tcttgccctc ggcatggccc tcgccgcgac gggtctggga
     1141 ctgcgtgccc gaagcgtggg ccgccgcgtt gtctcctggg tcatcatggg gatcgtcttc
     1201 cctctccttg tccccctcgt cggctttgcc ttcatggtga tcaaagggaa aaagggagag
     1261 gccgtcagcc cttccggggc aaagcccgtg agtatcccgg cccatgcggc gacggcattc
     1321 atcctgttcc tcctcgccgc cctcttcatg gactccctga gccttgcgct catctctttt
     1381 gtcatagccg tcatatgggt ctcaacccgc aaagaggacc ggtataaagg cagggtgcgg
     1441 ctggcggtga tcggtgtgta cgctttgatg gtcgtgatgg tcttcgcgat gaagggcatc
     1501 aactatcgcg tatcccaggc caacgcgagg gtgatcatag aggcgtgcga aagatacaag
     1561 ggcaggaccg gcgagtaccc ggccggattg agggacctcg tgcctgccta ccttccggag
     1621 gtccccgttg cgcgctacac cgtcatgtcc ggcgggttct attatcaccg gaatctcggc
     1681 ccgtcgggga gcgactacag gctggtgttc gtctacgagt ccccttttgc caggagcgtt
     1741 tacagttccg tggacagggg ctggcgcagc attgactaga tggatatttt gctgaattga
     1801 cagattttca caaaaaggca ggaacaatag cgaccgtaac ggtgtcgtag tgatatataa
     1861 gattgcgatc cactcagcgc acaaacgaaa aaggaggtat gttatggatt cgacgaacag
     1921 gagaaaggat cagatgagca gcatggcaag aaggacggtg ccttctttcg gctttgtatg
     1981 ctcaggtgct tcaaagggtg tctcaggggc atacacggcc gaggcagacg gttgcaaagg
     2041 cggctgcggg ggccagtgcc tgtcgggctg caaggacgag tgcatcacgg taagcaagta
     2101 ggtgccaccg gggcccgaga gccctgaaga gctccattcc attccggctt cagtccggta
     2161 gaggggcctg aagccggagc aacgaccgat aacgacatgg aaaagaccat cgccagagcg
     2221 cccgccgtgt ggcaggaatc catcgcaaag acggtgacct tcgtggtgac cgaggactgt
     2281 caactgcgct gccgttactg ctatctcgtc gggaagaact ccaggaaccg tatgagcttc
     2341 gatacggcaa agcggtcgat agactatctg gtggagaacc cggacctctt ccccgaaagg
     2401 tccatcgtct gggactttat cggcggcgag cccttcctgg agatcgacct catcgatgag
     2461 atatgcgagt acatcaagga gaaactctcc cgtgagaagc acccctggct cgacagccac
     2521 cggttcagcc tgacgacgaa cggcatactc tacggcagcg aaaaggtcca ggatttcata
     2581 agaaggaaca ggcccgacct cagcatcggt gtgaccatcg acgggacgcc gcgcaagcac
     2641 aacatgcaca ggatctaccc ttcggggaag gggtcgtacg acgacaccgc caggaacatc
     2701 cccctctggc tcgagcagtt ccccgtcgcc gccacaaagg tcaccgtcgc gagcgacgac
     2761 ctccccttca tccgggagag cgtcctccat ctgtatggcc ttggcatcaa gcacgtcagc
     2821 atcaacgtcg tcttcgagga cgtgtggaag gatggcgacg atgccatcct cgaggatgag
     2881 ctcgtgggtc tcgccgatgc cattatcgac ggggagttct tcacggacca cacctgctcc
     2941 ttcttctccg agacgatagg ccgtccgcgg acggacaaca acaactggtg cggtgccggc
     3001 aggatgctgg ccatcgaccc acggggagac ttctacccct gccacaggtt cacgcccttc
     3061 tctctcgtga acaggaagtt ccccccggtg ggcaactgct tcgaggggct cgacaggaac
     3121 aggctgcgcc cctttctggc ccttgacctt gtgagccaga gccgccccga gtgtatcgac
     3181 tgcgaggtcg cgacgggctg tgcctggtgt cagggggcca attacgactg tgccgaaacg
     3241 ggaacgatct accagagggc gacgcacctg tgcagaatgc acaaggcaag ggtgcgggcc
     3301 aacaactatt actggcacag gcttgaaggc gcccgccggc ccacgaggga ggcgcaatga
     3361 tacattacct catcgtcatc ctggagcatg attcggcccc gttttgctgc tacgatgtga
     3421 aggaaagggg cggacccggg cttatacccc tcgacgtgct ggaaaagagc gtctctttcg
     3481 cgataaagaa cgccataccc gtcaatttcc tctgcgggaa cacggccctg ccccccgggt
     3541 atgaagagct gatcgcaagc gtcgatcacg tgagaatggt tcctctcgcc cgcgcccggt
     3601 ccggggaaga ggccgtcttc atcatagacg gcgaagagga tcttgcccat gcccatgtcc
     3661 tcgagacggg cggcctcaac aacgtcattc tccgcgtcgg caagagtgag gtgccgtcgc
     3721 tcccctccac cgtggagaga cttctcggaa ggttcaggag gctcaacgtc atcctgaagg
     3781 aaccgggtgg atttgcggaa gaggactttt ccgcatatgc cgatgcactg gaagatgtcc
     3841 ggcgcatcct cgccggggag tatcgaaaag gcagcttcct cgagataagc accctttcgg
     3901 acagggtctt cctcacgaac atgaacaact gcaacgcagg gacggcccac ctgacggtcg
     3961 cgcccaacgg caggttctat ctctgccccg ccttctatta cgagaacggg gaggactgcc
     4021 ttgcggggcc cgatgatgcg gtggagataa ggaacgcccg gcttctcacc ctggagaatg
     4081 cccccgtctg ccgcaactgc gacgcctggc actgcaagag atgcgtctgg ctcaaccgca
     4141 ggctgacatc ggaactcaac acgccgtcgc atgagcagtg cgttcttgcc cacctggagc
     4201 gcgaggcgtc acggagattc ctggaagaga tcggccggga atgcggcctt aacgacaggg
     4261 tgacaccgat cccgaagctc gattacctcg accccttcga catcatcagc gaccggtcgc
     4321 gaagccccgg agaaaaggaa gaacacttcg cccggctcct ctcgcggccg ctcgaggacg
     4381 tccccgtgag cgacctgctc cgccagatct acaccctcga cccggggatg ctcgcgcgcc
     4441 tcaaagagtt gaactccgct gtcgtcgacc tcgaagacgg tcaatagcgg ggcccatgga
     4501 agcctcttcc gaattctcct cccggccccg ttccaccggc gagttcctgc gccggaccgt
     4561 cgtgtacctc aagccctaca aggcggccgc ggcggggaac atggcccttg cgttcctcac
     4621 gatcggcttc tactacgtat ttccctacgt cacgcagtac atcatc
//
LOCUS       DAOQTR010000105         4660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20536_length_4660_cov_4.137459, whole genome shotgun sequence.
ACCESSION   DAOQTR010000105 DAOQTR010000000
VERSION     DAOQTR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20536_length_4660_cov_4.137459"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKN85_03650"
     CDS             complement(<1..303)
                     /locus_tag="PKN85_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNT43529.1"
                     /translation="MSVTSEILEKIKKQDPHEFEFHQAAEEVLESLEPTAKKHPEFVK
                     AKIYERIVEPDRVVMFRVPWLDDKGELQVNRGFRIQFNNAIGPYKGGTRFHPSVNLG"
     gene            complement(479..694)
                     /locus_tag="PKN85_03655"
     CDS             complement(479..694)
                     /locus_tag="PKN85_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43530.1"
                     /translation="MRKRLVFVQRGKAEDTKALEDALKGHGWDLETVVLSKDEPLPRS
                     FENIDGLLITGSDLNVYDQSVTPLAVY"
     gene            complement(970..1623)
                     /locus_tag="PKN85_03660"
     CDS             complement(970..1623)
                     /locus_tag="PKN85_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT43531.1"
                     /translation="MTKRKILIVDDHPILRKGLTLLINQEPDLVVCAEAEHAQMALES
                     LERVVPDMAIVDISLPGIDGIELIKELHLRHRDMPVLVVSMHDETLYAERSLRAGARG
                     YIMKQEALEKVLVAIRKVLEGEIFVSDRITTKMLEKFVSVEGVSQTASPIDLLSNREL
                     TVFRLIGQGNKTRQIAERLHLSVKTVESYRAHIKEKLKLGDGTDLLKYAIQWVQSSP"
     gene            complement(1683..3236)
                     /locus_tag="PKN85_03665"
     CDS             complement(1683..3236)
                     /locus_tag="PKN85_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43532.1"
                     /translation="MHTRISEDRKKRAAGEDATPRALALALRRAEEKVRYLAERDRQH
                     KLAQRELEKRTHELNERVKELNCLYMISGIVEREGTTLDEILQKTVNMVPDAWQYPDI
                     ATCRIMLGDRTFVSGGFGETRWRQSQRIVVHGKDAGLLEVFYLQRRRKADEGPFLKEE
                     RSLIDAIAKRIGEIVERILGEEALKESESKMRALLLAIPDLMFQMDISGILLGLHPGD
                     FAILKSVAERLVGENIYLVSDREELLPRRLLDQVMSCVRQSISTGKAQVFEQHLSFGG
                     ETRDFEVRIVRCGPNKALGIVRDTTRRKRLEREILEISGREQRRIGQDLHDSLCQHLA
                     GIGFMGKVLERKTAVEQPIKPGDVAEIVDLIDQAITLTRGFARGLNPVRLEADGLMMA
                     LIELASNVERFFGITCAFEYDSPVLMSDNAAATHLYRIAQEALNNAIKHGKADNVTIA
                     MKQDGESCVLTISDNGVGMSGTQNQGRGMGLNIMQYRASMIGAVLEIREPKTGGCVIS
                     CTFSKRPAI"
     gene            3469..4587
                     /locus_tag="PKN85_03670"
     CDS             3469..4587
                     /locus_tag="PKN85_03670"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNT43533.1"
                     /translation="MLPELTIKGKSIQVPIIQGGMGIGVSLSPLASAVAAEGGIGIVS
                     SACLDRLLAKRNGKEYSTYDAVYEEVCLAREKGGVSGINVMVAIARDYDVTVKAAIDA
                     KVDIIISGGGLPLALPGIADPGDTALVPIVSSVRALQLICKKWERLGYRPDAVVLEGP
                     LAGGHLGFRVEDLGLESNRLENLFPPVKDFASRHGDFPVIVAGGIYDHHDISRFLAMG
                     ADGVQMGTRFLATVESSATEAYKHAVVNAGPRDIVVASTPGSPCGMPFMVIKESPMFL
                     SALGNKRKPRCDKGYIMTKDREGRYALCRAQENTGDCFCICNGLLSSGGYNSDVEEPL
                     YTVGANGYRVNRISTVRDVMDELKGSTPVTQLVSPEAA"
BASE COUNT          886 a         1422 c         1279 g         1073 t
ORIGIN      
        1 ccccaggttg accgagggat ggaagcgtgt gccgcccttg taaggtccga tagcattgtt
       61 gaactggatg cgaaaacccc tgttgacctg cagttccccc ttgtcgtcca gccaggggac
      121 cctgaacatc acaacgcggt cgggctcgac gatcctttcg tatatcttgg cctttacgaa
      181 ctcggggtgc ttcttcgccg tgggctcgag actctccagg acctcttccg ccgcctgatg
      241 gaactcgaac tcatgggggt cctgtttctt gatcttctcc agtatttcac ttgtaactga
      301 cataatggaa tcctccacct ctgtaatgta ttatcccggt cccttcccgg gctctcacgc
      361 aaaccatccc ggaccgggcc ggccggaagg ccgcaggcgg ccaccgcacc gccggtccgt
      421 cgcccgtatc cggcggcgag cctcgtggaa atcaaaagcc tgttcttcga gacattcctc
      481 agtagactgc caggggggtc acgctctgat cgtacacgtt cagatcgctc cccgtgatga
      541 gaagaccgtc gatgttctcg aagctcctcg gcaggggttc gtctttcgaa agaacgacgg
      601 tctccaggtc ccacccgtgc cctttgaggg catcttcgag ggctttcgta tcctcggctt
      661 ttccccgctg cacaaagaca agtctctttc tcatggtctc cgcttttctc cttttgatgg
      721 tatgagaaaa aactagtata taaaacccga taaaagcagg ttttacaatc agggcaggga
      781 tgtttttggc atcagggtga ctactatgca taccatagtg gttttcccca tgggggctgt
      841 gcagcgttga cggtcaacgg ttggcttgat cctgccgacg acggaacccg gcggaaaaat
      901 cttcggggga tagggggaaa atgcgggtcg gggcgagaag acccccgggg tctacgcggc
      961 catccttctt caggggctgc tctggaccca ttgaatggca tatttgagca ggtccgtccc
     1021 gtcgcccagt ttcagcttct ccttgatatg cgccctgtag gattccacgg tcttgacgct
     1081 gagatgaagt ctttccgcta tctggcgtgt tttgttcccc tgtcctatga gcctgaagac
     1141 ggtcagttcg cggttgctca ggaggtctat cggtgacgcg gtctggctga ccccttccac
     1201 ggagacgaac ttctcgagca tcttcgtcgt gatgcggtcg ctgacgaaga tctccccttc
     1261 gaggaccttt ctgatcgcga cgagcacctt ttccagggcc tcctgtttca ttatgtagcc
     1321 gcgcgctccc gcgcgaagcg aacgctccgc gtacagggtc tcgtcgtgca tggagacaac
     1381 gagaacaggc atgtccctgt gacgcaggtg caattccttg atcagctcga tcccgtcgat
     1441 gccgggcagc gatatgtcga cgattgccat atccggaacg accctctcca gtgattccag
     1501 ggccatctgc gcgtgctccg cctcggcgca cacgacaagg tcgggctcct ggttgatcag
     1561 aagcgtcaat ccctttcgca gaatgggatg gtcatcgacg atcaatatct ttcttttcgt
     1621 cacggatatc ctcccgtccc cttgtcaatg tccccgcaaa gcccgtcggc atgccgtcgc
     1681 cctcagatcg cgggtctttt ggagaaggtg cagctaatga cgcatccccc ggtcttcggc
     1741 tcccttattt ccaggacggc gccgatcatg gaagcccggt attgcattat gttgagcccc
     1801 ataccccttc cctggttctg cgtcccggac atgccgaccc cgttgtcgct gattgtcagc
     1861 acgcagcttt caccgtcctg tttcatggcg atggtcacgt tatctgcctt accatgtttt
     1921 atggcattat tcaatgcctc ctgggcgata cggtaaagat gggtcgcggc ggcgttatcg
     1981 ctcatgagga ccggggagtc gtattcaaag gcgcaggtga taccgaaaaa cctttccacg
     2041 ttcgacgcca gttctataag cgccatcatg agaccgtccg cctcgagcct gaccgggttt
     2101 aagccccggg cgaaacccct ggtcagggtt atcgcctggt cgatgaggtc cactatttcg
     2161 gcgacatccc cgggcttgat cggctgctcg acggcggtct ttctttccag caccttgccc
     2221 atgaaaccga tgcccgcgag gtgctggcaa aggctgtcgt gaaggtcctg acctatcctg
     2281 cgctgttcac gtccgctgat ctcgaggatc tctctttcca gccttttgcg cctcgttgtg
     2341 tcgcgcacaa tgcccagcgc cttgttggga ccgcacctta cgatccgcac ctcgaaatcc
     2401 cgcgtttccc ctccgaagga aaggtgctgt tcgaagacct gcgccttgcc tgtcgatatg
     2461 gactgcctta cacaggacat gacctggtcg agaagccttc gcggaaggag ttcctccctg
     2521 tcggatacga gatagatgtt ctctccgacc agtctttcgg caacgctctt caagatcgca
     2581 aagtcacccg ggtggaggcc gagaaggatc cccgatatat ccatctggaa catgaggtcg
     2641 ggaatggcca ggaggagagc gcgcatcttc gattcgctct ctttcagggc ctcctctccc
     2701 agtatgcgct cgacgatctc gccgatgcgc ttcgctatgg catcgatgag actcctctcc
     2761 tccttgagga aaggcccctc gtcggccttc ctcctccttt gcaggtagaa cacctccagg
     2821 aggcccgcat ccttcccgtg aaccacgatc cgctgggatt gcctccacct cgtctccccg
     2881 aagccgccac tcacgaaggt ccggtcgccc agcatgatgc gacacgtggc gatgtcggga
     2941 tattgccagg cgtcgggcac catgttgact gtcttctgca ggatctcgtc cagagtcgtc
     3001 ccctccctct cgacgatgcc cgatatcatg taaaggcaat tgagctcctt gaccctctcg
     3061 ttgagctcat gggtcctctt ctccagctcc ctctgcgcga gtttgtgctg cctgtcccgt
     3121 tccgcgagat acctgacctt ctcctcggca cgcctgagcg cgagggccag ggccctgggg
     3181 gtcgcgtctt cgcctgcggc ccgcttcttg cgatcttccg atatccgggt atgcatggtg
     3241 gcgtcattat atcataaaga ggggtgatgg gtgataggta ataggtgatg gagagcgtcc
     3301 tctcccatta cccatgaccc atgacccatt acctgcttct tcctcttcat ctattatttt
     3361 gtaattgacg gaaaaacatg ctatattgag cggtcgtgtt ttcattttta tttctttctc
     3421 tcagcttcaa caatccgtca taaaattgat tcacggcaca gagggatgat gctgccagaa
     3481 cttaccatca agggaaaatc catacaggta cccattatcc aggggggtat ggggatcggc
     3541 gtctccctgt cgcccctcgc gagcgccgtc gctgccgagg gcggcatcgg catcgtgtcc
     3601 agcgcgtgtc tcgacagact cctggcaaag cgcaacggca aggagtacag cacgtatgac
     3661 gccgtatacg aagaggtctg cctggcgcgg gaaaaaggag gcgtctcggg catcaatgtc
     3721 atggtcgcca tcgccaggga ctacgacgtc accgtgaagg cggccatcga cgcgaaggtg
     3781 gacatcatca tctccggagg cggactgccg cttgcccttc cgggaatcgc agatcccggg
     3841 gatacggccc tcgtcccgat cgtctcttcc gtgagggctc ttcagctcat ctgcaagaag
     3901 tgggaacgtt tgggataccg acccgacgcg gtcgtgctgg aagggcccct tgccggcggg
     3961 cacctcggtt tcagggtgga agacctcggc ctcgagtcca acaggctgga gaacctcttt
     4021 ccacccgtca aggacttcgc ttcacgccac ggcgatttcc ccgtgatcgt tgccggcggg
     4081 atctacgatc accacgacat ctcccggttc ctggccatgg gcgccgacgg ggtgcagatg
     4141 ggaacacgct ttctggccac cgtggagagc agcgcgacgg aggcgtacaa acatgccgta
     4201 gtcaacgccg gcccgaggga catcgtcgtc gcgtccaccc cgggctcgcc ctgcggaatg
     4261 cccttcatgg tgatcaagga atcccccatg ttcctctcgg cgctcggcaa caagagaaag
     4321 ccccgctgcg acaagggcta catcatgacg aaggacaggg aaggcaggta cgccctgtgc
     4381 agggcacagg agaacaccgg ggactgtttc tgcatctgca acgggcttct cagctccgga
     4441 ggctacaatt ccgacgtgga ggaaccgctt tacacggtgg gcgcgaatgg ctaccgcgtc
     4501 aaccgcatca gcaccgtgcg cgacgtcatg gacgaactca agggcagcac ccccgtcaca
     4561 cagctcgtat caccggaagc agcctaaaaa aaccgttcaa agctcaaggt tccagcaact
     4621 gtgttgaatg tcaattgatt tctttttcat gaacgggcct
//
LOCUS       DAOQTR010000106         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20570_length_4653_cov_3.397129, whole genome shotgun sequence.
ACCESSION   DAOQTR010000106 DAOQTR010000000
VERSION     DAOQTR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20570_length_4653_cov_3.397129"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKN85_03675"
     CDS             complement(<1..316)
                     /locus_tag="PKN85_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43534.1"
                     /translation="MSLQKVEFVERPGSVVRKCPGTRHHICCGYQTIDLVEGCVLSCS
                     YCILKAYLRDDTIRISHRPESIIAELDGMIDTNPRHILRFGTGELSDSLAIDRRYNVN
                     RFL"
     gene            complement(288..1220)
                     /locus_tag="PKN85_03680"
     CDS             complement(288..1220)
                     /locus_tag="PKN85_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNT43535.1"
                     /translation="MALHLFPAMMDVPLSRIGLKDGRFSLSYPRGDDSLRWSIAAIGV
                     VQPVILLDRTPYVPVVGWRRLQCARELRLKKVPALIVDIDEKTALLMAIHDNLARGFN
                     IIEKAAVVDAMDRFGFSRDEVFDIMAHLGLNPHEKVLSVLLKIASLDAPPKDFIFRKN
                     LSLRNAGSFLRFEEDERRKILAALSGLHLTESTVREVLEMLELIKIRKGTLTGRDIPV
                     LENPDMLRSHLKRKTHPILSSLAKKLKAIRGAMALPPGVDIRVDPFFEKEYIDIILKI
                     GSEDDVDIALGRISELVAAGHVRRILELTKGRIR"
     gene            complement(1193..1543)
                     /locus_tag="PKN85_03685"
     CDS             complement(1193..1543)
                     /locus_tag="PKN85_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43536.1"
                     /translation="MPYRRTSQWIVVVVSTLVTLFCLYVLLVSVRSGDMKSVWIFAAF
                     AFLFATPLFVTVFHFFADRSPTLARIHEEHISLGQKHKTTFIPHWFVMTGVIVIGIII
                     LYTVIKWLFIYFQR"
     gene            1665..2240
                     /locus_tag="PKN85_03690"
     CDS             1665..2240
                     /locus_tag="PKN85_03690"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNT43537.1"
                     /translation="MKRSLGARTVLLPAPVLVVGTYDKQGRPNVMTVAWGGMCCSNPP
                     CVDIALRKATYTYQNILDRKAFTVHVTPEKYLTEADYFGIVSGRDVDKFAATGLTAAG
                     SETVDAPYIKEFPFFLECALREVVELGLHTQFIGEIMDVKADEDVLNDRGLPEVGKIR
                     PTVMVPEIRNYYGFGERLGNVFIMGRKVRDR"
     gene            complement(2266..3099)
                     /locus_tag="PKN85_03695"
     CDS             complement(2266..3099)
                     /locus_tag="PKN85_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNT43538.1"
                     /translation="MGIITISRTHGSGGTTFARELAKELGYSYIDRTLINDECLESDR
                     HTCSFGIDEGTAPGLHGTTQELMADSNFYKVCFIASILDRALRDNAVMTGMGAGIILT
                     GIVNAINIRVVRLMEERVRAIAQVKGIPYDDAFDLVERMDEGKRDFIARYFDADPGDP
                     SYYHAVVNSSYVSLEEAVGILAEYARRHFTPDSAREAEGVLGDRLLEKRAEILLFYLG
                     MGHCFGQVTFRAGADGTLSVAGLVRNEAEKDRLLQALAKDARIRRITNSLETGVFEVV
                     S"
     gene            3350..4369
                     /gene="queA"
                     /locus_tag="PKN85_03700"
     CDS             3350..4369
                     /gene="queA"
                     /locus_tag="PKN85_03700"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNT43539.1"
                     /translation="MRIEEFDYDLPKEMIAQHPSGEREASRLLVCDREKGTMEHRQFS
                     DIAGYLREGDVLVINDSKVFPARLKARKKTGGMLDVLLVRSLGNGTWECLVRGMPRTV
                     NAIAVTVGDHGALLARGDDSWTISFVSPEEGDEVIGTLGRMPLPPYIKRDGGDGSADF
                     ERYQTVYAEKTGSIAAPTAGFHFSEELLGRITAEGVEIVRITLHIGIGTFSLIHPGPV
                     EEHRMHGERYEMDERSKGLIRAARQEGRRVVACGTSSVRTLETVFSMNGNATLAGETE
                     LFIYPGYRFRCVDALITNFHLPRSTPLMLAAAFMGAGPLKRAYAEAIRGGYRFYSYGD
                     AMFIS"
     gene            4366..>4653
                     /locus_tag="PKN85_03705"
     CDS             4366..>4653
                     /locus_tag="PKN85_03705"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HNT43540.1"
                     /translation="MKMIEIDHTDGGARSGRLITGHGAIETPVFMPVGTQGVVKAMTH
                     RQLEELGVTMILSNAYHLYLRPGDELIKRMGGLHNFSGWDGAILTDSGGYQI"
BASE COUNT          967 a         1273 c         1451 g          962 t
ORIGIN      
        1 cgaggaaacg gttcacattg taacgccggt cgatagccag actgtcggag agctcccccg
       61 ttccgaagcg aaggatatgc cgggggtttg tgtctatcat gccgtcgagt tccgcgatta
      121 tgctctcagg tctatgcgag atccgtatgg tgtcgtccct gaggtaggct ttgagtatgc
      181 aataggagca ggaaaggacg cagccctcca cgaggtcgat ggtctggtat ccgcagcaga
      241 tatggtgtct tgtgcccggg cactttctga caacgcttcc gggcctttca acgaattcta
      301 ccttttgtaa gctcaagaat tctcctgacg tgacctgccg cgaccagttc cgagatcctg
      361 ccgagcgcaa tgtcgacgtc gtcttcgctg cctattttca atatgatatc aatatactcc
      421 ttttcgaaga aagggtctac tctaatgtct acgccgggcg gaagggccat ggcgccccgt
      481 atggccttca gttttttcgc gagggacgac aggatggggt gggtcttccg cttgaggtgg
      541 gacctcagca tgtccgggtt ctccaggacg gggatgtccc tgcccgtgag cgttcccttg
      601 cggatcttta tgagctcaag catctcgagg acctcccgga ccgtgctttc cgtcaggtga
      661 agacccgaga gcgccgcaag gatcttcctt cgctcatcct cttcgaacct caggaaggac
      721 ccggcgttcc tcagggaaag gttcttcctg aagatgaagt ccttgggcgg ggcatcgaga
      781 gatgcgatct tgaggaggac ggataggacc ttctcgtggg gattgaggcc aaggtgggcc
      841 atgatatcga agacctcgtc gcgggaaaac ccgaaacggt ccatggcgtc gacgacggcc
      901 gctttttcaa tgatgttgaa gccccgtgcc agattgtcgt ggatggccat cagaagggcg
      961 gtcttctcgt cgatatcgac gatgagggcg gggacctttt tcaggcgaag ttccctggca
     1021 cactgaaggc ggcgccagcc gacgaccggg acgtagggcg tccggtcgag gaggatgacg
     1081 ggctgaacga ccccgatggc ggcgatcgac cacctgagac tgtcatcgcc ccgcggatag
     1141 gacagggaga accgcccgtc cttgaggcct atcctggaaa gggggacgtc catcatcgct
     1201 ggaaatagat gaagagccat ttgatgaccg tgtagaggat gatgatcccg atgacgatca
     1261 cgcccgtcat gacgaaccag tgaggtatga aggtcgtctt gtgtttctga ccaagggaaa
     1321 tgtgctcctc gtgtatcctg gcgagggttg ggcttctgtc ggcgaaaaaa tggaagactg
     1381 tgacaaagag cggcgtagcg aagagaaagg cgaaagcggc aaatatccac acgctcttca
     1441 tgtcaccgct tctgacactt acgagaagga cgtagagaca gaagagggtg accaatgtgg
     1501 agacgacgac cacgatccac tgggacgttc ttctgtaggg catgccacct tcctttttcg
     1561 gatgccattc attgtataaa agtatttcaa ggctgtcaat gatggggtat actagaaccc
     1621 gttcgcaggc ccgcctgttt gcgggtcatc aaggaggttc aggaatgaag agatccctcg
     1681 gcgcgcggac cgtcctctta ccggccccgg tcctcgttgt aggcacatat gataagcaag
     1741 gccgccccaa tgtcatgacg gtggcatggg gaggcatgtg ctgttccaat cccccctgcg
     1801 tcgacattgc cctcagaaag gcgacctata cgtatcagaa catcctcgac cgcaaggcct
     1861 ttaccgtcca cgtgaccccg gaaaagtatc tcaccgaggc ggattacttc gggatcgtct
     1921 ccggcaggga cgtggacaag ttcgccgcca cggggctcac ggcggcgggg agtgagacgg
     1981 tggacgcccc gtacatcaaa gaattcccct tttttctgga gtgcgctctc cgcgaggtcg
     2041 tcgagctcgg actccacact cagttcatag gcgagataat ggacgtgaaa gcggacgagg
     2101 atgtgctgaa tgacaggggg cttcccgagg tgggcaagat aagaccaacg gtcatggtgc
     2161 ccgagatacg caactactac ggatttggtg aaagactggg gaatgtcttt atcatgggca
     2221 ggaaggtccg cgacaggtag gcggccggcg gcctgaccgg cgtggtcagc tgacgacctc
     2281 gaaaacgccc gtctccaggg agttcgtgat cctcctgatc cgtgcgtcct tcgccagagc
     2341 ctgcaggaga cggtccttct cggcttcgtt ccggaccagt cccgccacgg atagcgtccc
     2401 gtccgctccg gcccggaagg tcacctggcc gaaacagtgc cccatgccca ggtaaaagag
     2461 aaggatctcg gcgcgtttct cgaggagacg gtcgccgagg acgccttcgg cttccctggc
     2521 ggagtcggga gtgaaatgcc tccgcgcgta ttccgccagt atcccgacgg cctcttcaag
     2581 ggagacgtag ctcgaattga ccacggcgtg ataataggag gggtccccgg gatcggcgtc
     2641 gaaataccgg gctatgaaat cccgtttgcc ctcgtccatc ctttcgacga ggtcgaaggc
     2701 atcgtcgtag gggatgccct ttacctgggc gatggcccgc acccgttcct ccatgaggcg
     2761 aacgacgcga atgttgatgg cgttcacgat accggtgagg atgatgcccg cgcccatgcc
     2821 cgtcatcacg gcgttgtccc ggagggcgcg gtccagtatg ctcgctatga agcacacctt
     2881 gtagaaattg gagtccgcca tgagttcctg tgtcgtgccg tgaagccccg gcgccgtccc
     2941 ttcatcgatg ccgaaggagc atgtgtgcct gtcgctctcg aggcactcat cattgatcag
     3001 ggttctgtcg atgtatgaat agccgagttc ttttgccagt tcccgcgcga atgttgtccc
     3061 gcccgagccg tgggttcttg atatcgttat aatacccatc gttgtccgaa agaattttaa
     3121 aatataagga tgcttttata caaggagaat ctcgcggggg tggccggtgc ggaaagagta
     3181 cgtgtaaccc attaattgat ttgcgaatat aggtataatc tgttataaga ttaacctgtt
     3241 tggagagggc cgggcggctc gaagggggtt ttttgctttg acagcccggg tggcggcagc
     3301 ggaatggcct gacacgaccc atatcacaac cgacggtact tgcggtgtta tgagaatcga
     3361 agaatttgat tatgatctgc cgaaggaaat gatcgcccag catccgtccg gggagcggga
     3421 ggcatcgcgc ctgctggtct gcgacaggga gaaggggacc atggagcatc ggcagttctc
     3481 ggatattgcc ggctatctgc gggaaggcga tgtcctcgtc atcaacgaca gcaaggtgtt
     3541 tccggcgcgg ctcaaggcgc gcaagaagac gggcggcatg ctcgacgttc ttctcgtcag
     3601 gtccctgggc aacggcacgt gggaatgcct ggtgcgggga atgccgcgga cggtgaacgc
     3661 gatcgccgtc accgtgggag accacggcgc cctccttgcg cgcggggacg attcctggac
     3721 aatatccttc gtgtcccccg aggaaggcga cgaggtcata gggaccctgg ggaggatgcc
     3781 ccttccacca tatatcaagc gcgacggggg ggacggttca gccgatttcg aacgctacca
     3841 gacggtgtac gccgagaaga ccgggtccat agcggcgccg acggctggtt ttcacttttc
     3901 cgaagagctc ctcgggcgca tcacggcaga aggggtggag atcgtgagga tcacactcca
     3961 cataggcatc gggacattct cgctcatcca ccccggtccc gtcgaggagc acaggatgca
     4021 cggcgagcgc tacgagatgg acgagagatc gaaggggctc atccgggcgg cgcggcagga
     4081 aggacgccgc gtggtcgcct gcggcacgag ttcggtgagg accctcgaga cggtattcag
     4141 tatgaacggc aacgccacgc tcgccgggga gacggagctt ttcatctatc cgggctaccg
     4201 ttttcgctgc gtcgacgcgc tgatcaccaa ttttcacctt ccccgttcga cgccgctcat
     4261 gctcgccgcc gccttcatgg gcgcaggacc gctgaagagg gcctatgccg aggcgatacg
     4321 cgggggatac cggttctata gttacgggga cgcgatgttc atatcatgaa gatgatagag
     4381 atagaccata cagacggagg ggcgcgttct ggaaggctca tcacgggtca cggggcgatt
     4441 gagacgcctg ttttcatgcc cgtgggcacc cagggggtcg tcaaggccat gacccaccgg
     4501 cagcttgagg aactgggcgt cacgatgatc ctgtccaacg cctaccacct ctacctgcgc
     4561 cccggcgatg agctcatcaa aaggatgggc ggcctccaca atttctccgg gtgggacggc
     4621 gccatcctca ccgatagcgg aggttaccag ata
//
LOCUS       DAOQTR010000107         4646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20606_length_4646_cov_4.149641, whole genome shotgun sequence.
ACCESSION   DAOQTR010000107 DAOQTR010000000
VERSION     DAOQTR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20606_length_4646_cov_4.149641"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1026
                     /locus_tag="PKN85_03710"
     CDS             241..1026
                     /locus_tag="PKN85_03710"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT43541.1"
                     /translation="MPDYSKIDIPEVLSYVFYPRDESGPCPRYAFDHFIPVAEAVALH
                     CRFYEQDKAWPWILYFHGNGEVVSDYDEIALFYFKHNLNLAVVDYRGYGKSNGTPTVA
                     DMSRDSRKVYESVKTALTQRGLRDDLWVMGRSLGSVSALAIAFDKSTGINGLIIESGF
                     PSITSLIVRHGIATPGMDLDAITRECLDMVRSITVPVLIIHGEYDTLVPPDEAETIYE
                     TIGSARKDLLMIPGATHNDIMFVGLRQYMEAIRKFVDSTSPNR"
     gene            complement(1062..1244)
                     /locus_tag="PKN85_03715"
     CDS             complement(1062..1244)
                     /locus_tag="PKN85_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43542.1"
                     /translation="MVTCNMDDDEAKLLLNVLERYHSHLEVEIVRTNRREFRDALKER
                     EKQLSGLMEKVKALVK"
     gene            complement(1284..1757)
                     /locus_tag="PKN85_03720"
     CDS             complement(1284..1757)
                     /locus_tag="PKN85_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT43543.1"
                     /translation="MARKKEVIGVSIMEASPTKEFDLFQGVSQRVIGEIGRLGEEVSF
                     DRDDIVFKADEPGSFFYELVEGEVDIIVLEKENMHFIVKRPGEIFGWSALVEPYVYSA
                     TARCLAPTRAIRIGRDVIENVVKRHPEEGLIIYKHIIGIVAQRLRSAYQYIYRQQ"
     gene            2009..2428
                     /locus_tag="PKN85_03725"
     CDS             2009..2428
                     /locus_tag="PKN85_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNT43544.1"
                     /translation="MKRFDVDNLSSLVIRAAINVHKVFGPSLLESIYQSSMVIELGLM
                     GIEVLTEVPLHVSYKGRKIPNVGLRLDLLVEDTIIVELKSVEEIQPVHKKQLLTYLRL
                     AGKPLGLLINFNTTLLREGITRVISSPQGDGNAARFR"
     gene            complement(2508..3920)
                     /gene="aspA"
                     /locus_tag="PKN85_03730"
     CDS             complement(2508..3920)
                     /gene="aspA"
                     /locus_tag="PKN85_03730"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNT43545.1"
                     /translation="MSNEVRKEHDLLGEREIPADAYYGVQTLRATENFRITGIPLSKY
                     PAFVSSLAYVKKAAALANGELGLLPPDVADAIRRACDDVLEGKHREHFVVDMIQGGAG
                     TSTNMNANEVIANIALEKLGYEKGRYDVVHPNNHVNLCQSTNDVYPTAIRLTLILKIK
                     DLVEAMDYLRSAFEKKSKEFSQVIKMGRTQLQDAVPMTLGQEFGAWAIMLAEDMDRMR
                     EAQALVKEINLGATAIGTGLNAHPDYSSLVAEKLRQITGIDVLTSPDLVEATQDSGAY
                     VQLSGVLKRFAVKISKICNDLRLLASGPRCGLNEINLPPMAPGSTIMPGKVNPIIPEV
                     VNQVAFFVIGNDVTVSFAAEAGQLELNVMEPVMAFSLFNSLVALRRASKTLADRCVLG
                     ITANQDKCREYVECSIGLVTALNPYIGYERSTEIAHEALATNRSVCELVLEKGYLKKE
                     ELDDILSPDNMIKPRYMEKK"
     gene            complement(3917..>4646)
                     /locus_tag="PKN85_03735"
     CDS             complement(3917..>4646)
                     /locus_tag="PKN85_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNT43546.1"
                     /translation="FKYTNIVMRFAPVGVGAAIAVVVASKGMNVLLNLCLLIASFYGA
                     IVIFMLGVLLPVALLFKVPVRRFIAAVKEPAIIAFSTSSSEAALPKAMENMEALGVPR
                     RVVSFVIPTGYSFNLDGTTIYLSLASIFAAQAAGINLGLSEQLLIGFTLIFASKGAAG
                     VPRASLVVLAGTLASFGLPIQAVAVILGVDTVMDMGRTAVNVTGNCLATVIVAKWEKV
                     FDKKEETPGEGSLDDGKTLSEASL"
BASE COUNT          950 a         1383 c         1303 g         1010 t
ORIGIN      
        1 ctcgtcggcg cgaaggtcct cgtcgccgtc ctcgtgggca ggacgaaaaa agttctaacg
       61 ggaaggatct accggtggat catgcggttt ctcggcctcg ccctcttcct gtttgccctc
      121 tttctcgtca gggacggcct tgttctcctc acagtgatcg ccaaatttcc ttgaaagcgc
      181 ggcatcggga cggatagaat atccaagtct gcaaaatcct tatcgagagg agcgaacgtc
      241 atgcccgatt acagcaagat agacataccg gaggtcctgt cctacgtgtt ctacccgcgt
      301 gacgagtccg gcccctgtcc gagatacgcc ttcgaccact tcatccccgt ggcggaagcg
      361 gtggctctcc attgccgttt ctacgagcag gacaaggcct ggccctggat cctctatttc
      421 cacgggaacg gcgaggtcgt gagcgactac gacgagatcg ccctcttcta cttcaagcac
      481 aacctcaacc tggcggtggt cgattaccgg ggttacggga agtccaacgg cacgcccacc
      541 gtcgcggaca tgtcccgtga ttctagaaag gtctacgaat cggtcaagac ggccctcaca
      601 caaaggggtt tacgggacga cctgtgggtc atgggcaggt ctctcggcag cgtttccgct
      661 ctggcgatcg ccttcgacaa gagtaccggc atcaatgggc tcatcatcga aagcggtttc
      721 cccagcatca cgagcctcat cgtccgccac ggcatagcca caccgggcat ggacctcgac
      781 gccattaccc gggagtgcct cgatatggtg cgatcgatca cggtccccgt cctcatcatt
      841 cacggggagt acgacaccct tgtgccgccc gacgaggccg agaccatcta cgagaccatc
      901 ggttcagcgc ggaaggacct cctgatgata ccgggggcga cgcacaacga tataatgttc
      961 gtgggcttgc ggcagtacat ggaggccatc aggaagttcg tcgattcgac gagccccaac
     1021 cgatagcctc agtcggtctt cggcttcgcg gacagccccc cctactttac gagggccttc
     1081 accttttcca tgagccccga tagctgtttt tccctctcct tgagggcatc acggaactcc
     1141 cgcctgttgg tccttactat ctccacttcc aggtgggaat ggtacctttc aagcacgttg
     1201 agtaagagtt tggcctcgtc gtcgtccata ttgcacgtaa ccatacattc ctcctttaca
     1261 ggcctggagc cgtgcggcgg ccgtcattgc tgcctgtaga tgtattgata tgcgcttctg
     1321 agcctctgcg cgacgatccc tatgatgtgt ttatagatga tgagaccctc ttcgggatga
     1381 cgtttcacga cgttttcaat gacatccctc cctattctta tggccctcgt cggcgccagg
     1441 caccgggccg tggcgctgta gacgtagggt tccacgagcg ccgaccagcc gaatatctcg
     1501 ccaggtctct tcactatgaa atgcatgttc tccttttcca gcacgatgat atccacttct
     1561 ccttccacca gttcataaaa gaacgacccc ggttcatccg ccttgaaaac aatgtcgtcg
     1621 cgatcgaagg acacctcctc tcccagcctc ccgatctccc cgataaccct ctgactgact
     1681 ccttgaaaaa gatcgaattc ctttgtgggg gacgcttcca tgatagacac gccgatcacc
     1741 tccttctttc gtgccaccct tatactctat ttagcacaac ttcggaataa gacaaggaac
     1801 aagacaaaga cctatctcac gcccgttcgt tcctcactca agcccgcaga ggacacagag
     1861 aaatgcgcaa agaattgcgg ccagattccg gagcctacca gaatctggcc gcacttgcat
     1921 cccgtcttcg acgggaggca tcccgccgcc acgctggcgg gacaaaaaac ccgagattcg
     1981 aagtaatacg ctcacacgag gtgttggtat gaaaaggttt gatgtggata atctgtcgtc
     2041 gctggtcatc agggccgcaa tcaatgtaca taaggtgttc ggaccaagcc ttcttgagag
     2101 catctatcag tcctctatgg tcatagagct ggggctgatg ggcatagagg tcctgacgga
     2161 agttcccctg cacgtgtcct ataaagggcg gaaaattccc aatgtcggtc tgaggctgga
     2221 tctgctggtg gaggacacga tcattgtgga gctaaagtcc gtcgaggaga ttcagccagt
     2281 ccacaagaag caattattga cgtacctgag attggcagga aaacccttag gactgctaat
     2341 caacttcaac acaaccctct tgagggaagg cataacaagg gtgatcagct cgccgcaagg
     2401 cgacggaaat gcggccagat tccggtaggc tccggaatct ggccgccatc ttttctttgc
     2461 gtcctccgcg ggcttgagtg aggaacgaac gggcgagagg ccgtcttcta cttcttctcc
     2521 atatacctcg gtttgatcat gttgtccgga gagaggatgt cgtcgagttc ttcctttttc
     2581 aggtagccct tttcgaggac gagctcgcag accgagcggt tcgttgccag ggcctcgtgg
     2641 gctatctcgg tggagcgctc gtagccgatg taggggttga gggccgtgac gaggcctatg
     2701 ctgcactcga cgtattcgcg gcacttgtcc tggttcgccg ttatcccgag gacgcagcgg
     2761 tcggcgaggg tcttgctggc acgcctcagg gccacgaggg aattgaagag actgaaggcc
     2821 atgacgggtt ccatgacgtt cagctccagc tgccccgcct ccgcggcgaa gctcaccgtc
     2881 acgtcgttgc cgatgacaaa gaatgccacc tggttcacca cctcgggaat gatggggttc
     2941 acctttccgg gcatgatcgt cgagccgggg gccatgggag ggaggtttat ctcattgagg
     3001 ccgcagcgcg gaccggacgc caggagcctc aggtcgttgc agatcttgga gatcttgacg
     3061 gcgaagcgct tcaggacgcc cgacagctgg acataggcgc ccgaatcctg tgtcgcctcc
     3121 accaggtcgg gtgatgtgag cacatcgatc cccgtgatct ggcggagctt ttccgcgaca
     3181 agggaagaat aatcaggatg ggcgttgagc cccgttccga tggcggtcgc tcccagattt
     3241 atctccttca cgagcgcctg cgcctccctc atccggtcca tgtcctcggc aagcatgatg
     3301 gcccaggcgc cgaattcctg gcccagcgtc attggcacgg catcctgaag ctgcgtcctg
     3361 cccatcttga tgacctggga aaactctttc gatttcttct cgaaggccga gcgcagatag
     3421 tccatcgcct ccacgaggtc cttgatcttc aggataaggg tcagtcgtat cgccgtcggg
     3481 tagacatcat tggtcgactg gcagagattg acgtggttgt tggggtggac aacgtcgtac
     3541 cgtccctttt cgtatcccag cttctcgagg gcgatgttgg cgatgacctc gttggcgttc
     3601 atgttcgtgg atgtgcctgc cccgccctgt atcatgtcaa cgacgaaatg ttccctgtgt
     3661 ttcccctcga gcacatcatc gcaggcccgg cgtatcgcgt ccgccacgtc agggggcagg
     3721 agcccgagtt ctccgttcgc aagggcggcg gccttcttca cataggccag cgaacttaca
     3781 aaggcgggat atttggacag gggaatgccc gtgatccgaa agttctccgt tgcccgcagt
     3841 gtctggacgc catagtacgc atcggcggga atctcccgct cgccgagaag atcgtgttcc
     3901 ttcctcactt cattgctcat agcgacgcct ccgatagtgt ctttccatca tccagggacc
     3961 cctccccggg ggtctcctcc ttcttgtcga ataccttctc ccacttcgcg acgatgacgg
     4021 tggcaaggca attgcccgtg acgttcaccg ccgttcgtcc catgtccatc accgtatcca
     4081 cgccgaggat gaccgccacg gcctgaatgg gcaggccgaa cgacgccagg gtgcccgcga
     4141 ggacgacaag ggacgccctg gggacgccgg cggctccctt gctggcaaag atcagggtaa
     4201 agcctatgag gagctgctcc gacaggccaa ggttgatgcc ggccgcctgc gcggcaaaga
     4261 tggacgcgag agacagatag atggttgtgc catccaggtt gaaactgtat cccgtgggaa
     4321 tgacgaagga gacaacccgt ctcggcaccc cgagggcctc catgttctcc atcgccttcg
     4381 gaagggcggc ctccgaactg gacgtggaga acgcgataat ggcgggctcc ttgacggccg
     4441 cgataaatct ccttacgggg accttgaaaa ggagcgccac gggcaggagc acacccagca
     4501 tgaagatcac gatggccccg taaaagctgg ctatgaggag gcacaggttg aggagcacgt
     4561 tcatcccctt gctggcgaca acgacggcta tggccgcccc gacgccgacg ggcgcgaagc
     4621 gcatgacgat attggtgtac ttgaac
//
LOCUS       DAOQTR010000108         4646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20608_length_4646_cov_3.260510, whole genome shotgun sequence.
ACCESSION   DAOQTR010000108 DAOQTR010000000
VERSION     DAOQTR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20608_length_4646_cov_3.260510"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PKN85_03740"
     CDS             complement(<1..709)
                     /locus_tag="PKN85_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNT43547.1"
                     /translation="MEENITIEFAGRPLTISAGGLAKQADGSVMARYGDTVVLVTAVA
                     EKQARDKDFLPLTVNYQEMSYAAGKFPGGFFKREGRPTTREILTSRLIDRPLRPLFPK
                     NWRYETQVISTVLSADQDNDPGVLSLVGASCAVTISDIPHDGPFAGVRVGRIGGEFVV
                     NPTFPQMADSDIDIIVTATRDAIIMVEGEAHFVKGTDLIEAIEFGHQAMMPLIEMQER
                     LREKIGKQKRQIPVPENL"
     gene            complement(728..997)
                     /gene="rpsO"
                     /locus_tag="PKN85_03745"
     CDS             complement(728..997)
                     /gene="rpsO"
                     /locus_tag="PKN85_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNT43548.1"
                     /translation="MPLDTQRKKSIIEDFKTHDTDTGSPEVQIALLTERIRSLTEHFK
                     KFSKDHNSRRGLLVLVGSRRRLLNYLKDKNVDRYKKVVEKLGLRK"
     gene            complement(1008..1892)
                     /gene="truB"
                     /locus_tag="PKN85_03750"
     CDS             complement(1008..1892)
                     /gene="truB"
                     /locus_tag="PKN85_03750"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNT43549.1"
                     /translation="MNGYLIIDKKAGMSSYDVIRRLKRLHKFKKIGYIGTLDRNATGI
                     LPVALDEGVKLIPFLENVEKQYRARFLLGVTTETQDIEGKVLTETPTEPFDRQVIEDA
                     LESYLGRITQRVPAYSSKKVNRKPLYKWARSGVQVEQPVKEVEIFDITLLDYTHPHLD
                     VEVTCSKGTYIRALASDLGEKLGCGATLFTLKRTRHGEFSEGMGTDIDCFKMEQDLLN
                     YLLPLENILGSVRGMVVETALEKFLRHGMPVPIAGSAREWRHGEAARLFNRQGALIAV
                     GIADLGSKTIKIKRLINY"
     gene            complement(1953..2915)
                     /locus_tag="PKN85_03755"
     CDS             complement(1953..2915)
                     /locus_tag="PKN85_03755"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNT43550.1"
                     /translation="MKAKVRRLIEEGCSFLVTTHIDPDGDAIGSVFAFAMALGTLGKE
                     ATVYLRDRIPYRYEFLPAPGRVVHAVPEGKYDAVFVLDCGSLFRVGAGHDRIGAMAPI
                     VNIDHHDTNDHFGSVNLVDPGASSTGEIIYRLFRSLKVKVDPDMAVNLYTAIFTDTGS
                     LRYDNTSLEAFRICEEMVKAGVRPSWVAGMVYENHPKERFLLLGEVLCTLTTYDKKIA
                     VARVTSDMFERTGTNREFTDGFVEFIKEIRGVEVAVLIREIDEHHHKVSMRGKGAVDV
                     AAVCNSFGGGGHRNAAGCRIEGTMEETEEKLLRTLAPEGWVKDE"
     gene            complement(2899..3249)
                     /gene="rbfA"
                     /locus_tag="PKN85_03760"
     CDS             complement(2899..3249)
                     /gene="rbfA"
                     /locus_tag="PKN85_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNT43551.1"
                     /translation="MRYRRLRVQDLLREEISLIIQRELQDQGLGFITVVEVKMSEDLK
                     SAKVYVSIYGSEEEQKHTFEALKRSKGYIKFLLGKRVQLRYMPEITFLLDDTLERVQR
                     MEEIFRKEADEGKG"
     gene            complement(3246..3545)
                     /locus_tag="PKN85_03765"
     CDS             complement(3246..3545)
                     /locus_tag="PKN85_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNT43552.1"
                     /translation="MVVGVSRVDIFFPENHSLKDKRQMLRKVIEKTRAKFNISMIEVD
                     QSNLWQRARIGFAVAGVRQDHVSKVIENVHEYMESLYLGEVIDTSSEIIVIGDEI"
     gene            complement(3551..>4646)
                     /gene="infB"
                     /locus_tag="PKN85_03770"
     CDS             complement(3551..>4646)
                     /gene="infB"
                     /locus_tag="PKN85_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNT43553.1"
                     /translation="TLFAKISAKKKEGIKELLELIVLQSEMLELKANPDKLARGTVIE
                     SELDRGHGPVGTVIIQDGTLKIQDPFISGMTFGRVRAMIDDKGARIQVAPPATPVLVV
                     GFQDVPHGGDRFIVTTEERYAKELSKFRQEKLREREALKSARTTLEDLYSKIGETEKV
                     FLNVIVKGDVRGTIDAIVEALKKMSTDAVEVQITHSGVGAITETDVNLAMASGSIIIG
                     FNVRPVAKAQALAEQEKIEVRQYSIIYDMIDDVKKAMEGMLAPKIVETTIGKADVRKT
                     YSVSRIGIIAGSYVTEGKATRNSLIRVVRGGQIIHTGKIASLKRFKDDVKEVQSGYEC
                     GISLEDYNDIQEGDVFEFFIEEKEKQTLDG"
BASE COUNT          835 a         1476 c         1223 g         1112 t
ORIGIN      
        1 cgaggttctc ggggaccggt atctgcctct tctgctttcc gatcttttcg cggagcctct
       61 cctgcatctc gatgagaggc atcatggcct ggtggccgaa ctcgatggcc tcgatgagat
      121 cggtgccttt cacgaaatgc gcctcgccct ccaccatgat gatggcgtcg cgggttgccg
      181 tgacgatgat atcgatatcg ctgtcggcca tctggggaaa ggtcgggttc acgacgaact
      241 caccgcctat ccttcccacc ctgacacccg cgaaaggccc gtcatgaggg atatccgata
      301 tggtgaccgc gcaggacgcc cccacgaggc tcaggacgcc cgggtcgttg tcctggtcgg
      361 cggaaagcac cgtcgagatc acctgcgtct cgtatcgcca gttcttcggg aacagaggcc
      421 tcaacggcct gtcgatgagg cgcgatgtga gtatctcccg ggtggtcgga cgtccttccc
      481 tcttgaagaa acccccgggg aacttcccgg cggcgtaaga catctcctga tagttgacgg
      541 tgagcggcag aaagtccttg tccctcgcct gtttttcggc aacggcggtg acgaggacaa
      601 ccgtgtcccc gtagcgcgcc atgacgcttc catcggcctg tttcgccaaa cccccggcgc
      661 tgatagtcag cggtcggccc gcgaattcga tagttatgtt ttcttccata tttttcctat
      721 tattccttta ttttctaaga ccgagcttct ccacgacctt cttgtagcgg tcaacgttct
      781 tgtccttgag atagttgaga agccgtcttc tgcttccgac aaggacgaga agccctcttc
      841 tcgagttgtg gtccttggag aacttcttga aatgctccgt cagggatcgt atccgctccg
      901 tgagaagcgc tatctgcact tccggggaac cggtgtcggt atcatgggtc ttgaaatcct
      961 caatgatcga ctttttcctc tgagtatcca gtggcatata ttcctcctta ataattgatc
     1021 aatcgtttta tctttatcgt tttggacccc aggtccgcga tacccacggc gatcagcgcc
     1081 ccctgcctgt tgaagaggcg cgcggcctcg ccgtgcctcc actccctcgc gctgcccgct
     1141 atcggcacgg gcatcccgtg tctcaggaac ttctccaggg ccgtctctac caccatgccc
     1201 cgcacggaac ccaggatatt ttccaaaggt aacaaatagt ttaacaggtc ttgctccatt
     1261 ttgaaacagt caatatcagt tcccatgccc tccgagaact ctccgtgtct cgtccgtttc
     1321 aaagtgaaga gcgtggcccc gcacccgagc ttctcgccga ggtcgctcgc cagcgccctg
     1381 atgtaggtac ccttggaaca ggtgacctcc acatcgagat gagggtgggt gtaatcgaga
     1441 agggtgatat cgaagatctc cacctccttg acgggctgct ccacctgcac tccgcttcgc
     1501 gcccacttgt agaggggttt gcggttgacc tttttcgagg aataagcggg aacccgctgg
     1561 gttatcctgc ccaggtaaga ctcgagcgca tcctcgatga cctgccggtc gaaaggctcc
     1621 gtcggcgttt ccgtgagcac cttgccctcg atgtcctggg tctccgtcgt cacgccgagg
     1681 aggaatctcg cgcggtactg cttctctacg ttctcgagga aaggtatgag tttgacccct
     1741 tcatcgaggg cgacaggcag gatgcccgtc gcgttgcggt cgagggtgcc gatgtaccct
     1801 attttcttga acttgtggag cctcttcagc cgtcgtatca catcgtagga ggacatcccc
     1861 gccttcttgt cgattatcaa gtatccgttc attccctgta tccgtttcct tccttcttcg
     1921 tcccgccgcc caattcgggc ggcaccggtc ttctattcgt ccttcaccca gccttcgggc
     1981 gcgagggtgc gaaggagttt ctcttccgtt tcctccatgg tgccctcgat gcggcacccg
     2041 gcggcattgc gatgcccccc gccgccgaag gaattgcaga cggcggcaac gtccacggcg
     2101 cccttgcccc gcatgctgac cttgtggtgg tgctcgtcga tctcccgtat cagcaccgcg
     2161 acctcgacac cgcgtatctc ctttatgaac tcgacaaaac catcggtgaa ctcgcggttc
     2221 gtccccgtcc tctcgaacat gtccgacgtc acgcgggcca ccgctatctt cttgtcgtac
     2281 gtggtgagcg tgcacaggac ctcaccgagg agtaggaacc tttccttggg gtggttctcg
     2341 tagaccatcc ccgccaccca tgacggcctc acgcccgcct tcaccatctc ttcgcatatc
     2401 ctgaaggcct cgaggctcgt gttgtcgtac cgaagcgaac cggtatcggt gaagatggcc
     2461 gtatagaggt tgacggccat gtcggggtcc accttgacct tcagggacct gaagagccgg
     2521 taaatgatct ctcccgtcga cgacgccccg ggatcgacaa ggttcacaga cccgaagtgg
     2581 tcgttcgtgt cgtgatggtc gatattgacg atcggtgcca tcgcgccgat ccggtcgtgc
     2641 cccgccccga cccggaaaag gctgccgcag tcgaggacga agacggcgtc gtacttgcct
     2701 tcgggtaccg catggaccac cctgcccggc gccgggagaa actcgtacct gtacgggatc
     2761 ctgtccctga ggtagaccgt ggcctccttg ccgagcgtgc ccagggccat ggcaaaggcg
     2821 aaaacggacc cgatggcatc tccgtccgga tcgatgtggg tcgtcacgag gaaactgcaa
     2881 ccctcttcga tgagcctcct aacctttgcc ttcatcggcc tccttcctga atatctcctc
     2941 catcctctgc accctttcca gggtgtcatc gaggagaaag gtgatctccg gcatgtacct
     3001 caactgcacc ctcttgccaa ggaggaactt tatgtaaccc ttggaacgct tcaaggcctc
     3061 gaaggtgtgc ttctgctcct cttcgctccc gtatatggaa acgtagacct tggcggactt
     3121 caggtcctcg ctcatcttca cctcgacgac ggtgatgaag ccgagaccct ggtcctgcag
     3181 ctcccgctgg atgataagcg atatctcttc gcggagcagg tcctggaccc tcaggcgccg
     3241 gtatctcata tctcatcccc gataacgatt atctccgacg aagtatcgat cacctcgccc
     3301 agatacagag attccatata ctcatggacg ttctctatga ccttgctcac gtgatcctgc
     3361 ctcacgcccg cgacggcgaa accgatcctg gcgcgctgcc acaggttcga ctggtccacc
     3421 tctatcattg agatgttgaa tttcgcccgt gtcttctcga tcactttcct cagcatctgc
     3481 cgcttgtctt tgagagagtg attctcagga aagaaaatgt cgacgcggga tacaccaact
     3541 accataacct tcaaccatca agggtctgct tctccttttc ctcgataaaa aattcgaaga
     3601 catccccttc ctgtatgtcg ttgtaatcct cgagggaaat accgcattcg tatcccgact
     3661 ggacttcctt gacgtcatcc ttaaagcgct tcagggaggc gatcttcccc gtatggatga
     3721 tctgtccgcc ccggacgacc ctgatcagcg agttgcgcgt cgccttgccc tcggtcacgt
     3781 aggaaccggc aatgatgccg atgcgggaca cggagtatgt cttccgcacg tccgccttgc
     3841 ctatcgtggt ctccacgatc ttcggggcca gcatgccttc cattgccttc ttcacatcgt
     3901 ctatcatgtc atagatgatg gagtactgcc tgacctctat cttctcctgc tccgccagcg
     3961 cctgcgcctt cgcgacgggc cgcacgttga accctatgat gatggagccc gacgccatgg
     4021 caaggttcac gtcggtctcc gtgatcgccc cgaccccgct gtgggttatc tggacctcga
     4081 cggcatccgt ggacatcttt ttcaacgcct cgacgatcgc gtcgatcgta ccgcgcacat
     4141 cacccttgac gatgacattg agaaacacct tctccgtctc gccgatcttc gaatagagat
     4201 cttccagggt tgtcctggcg ctctttaagg cctccctctc gcggagcttc tcctgcctga
     4261 acttcgagag ctccttggcg tagcgctcct ccgtggtgac gatgaagcgg tccccgccgt
     4321 ggggcacgtc ctggaaacca acgacgagca cgggggtcgc cggcggggcg acctggatcc
     4381 tggctccctt gtcgtcgatc atggcccgca cgcggccgaa ggtcattcct gagatgaagg
     4441 ggtcctgtat cttgagggtg ccgtcctgga tgatgacggt ccccaccggt ccgtggcccc
     4501 tgtcgagctc gctctcgatg acggttcccc gggccagctt gtccgggttc gccttgagct
     4561 ccagcatctc cgactgcagg acgataagct ccagcaactc tttgatgccc tctttcttct
     4621 tggccgatat cttcgcgaag agcgtc
//
LOCUS       DAOQTR010000109         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20765_length_4619_cov_3.318580, whole genome shotgun sequence.
ACCESSION   DAOQTR010000109 DAOQTR010000000
VERSION     DAOQTR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20765_length_4619_cov_3.318580"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKN85_03775"
     CDS             complement(<1..239)
                     /locus_tag="PKN85_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HNT43554.1"
                     /translation="MQNDRFTVLFLGDVIGKPGRKAVGRYLEETRADFVVINGENLAG
                     GIGITTRTAVEMLSLGVDAITTGNHVWKKKEVVPFL"
     gene            complement(229..1782)
                     /gene="rny"
                     /locus_tag="PKN85_03780"
     CDS             complement(229..1782)
                     /gene="rny"
                     /locus_tag="PKN85_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNT43555.1"
                     /translation="MNTIVIAVIAFVIALFIGFFLTKLIHQKKVGEYDKIGKKILDDA
                     KKEADVVMREASIQAKDIVLQARNEIEQEVKARKVEMQNTERRLSQKELNIDKKLDAL
                     EKKEEELSRKDREMSNRYNHLEGRIREQETLVARSKEQLEKISGMTGEEAKKMLVTAM
                     EEEARYESMKMIKKIEEEAREKADKKAKEIIAFAIQRYAGEYVGEDTVSVVPLPNEEM
                     KGRIIGREGRNIRALEAATGVDIIIDDTPEEVILSCFNPVRREVARIAIAKLISDGRI
                     HPARIEEIVSKVAEEMEEKIKEAGEQAVFDLGIHNVHPELVKLLGRLKFRSSYAQNIY
                     QHSLEVAFICGAIASELKVSVKEAKRAGLLHDIGKAVDHEIEGSHATIGADLARKYGE
                     SDEIIHSILAHHEDVEVKSLLDVIVQAADALSGARPGARREMLETYIKRLQELEKIAN
                     TFQGVEKSYAIQAGREIRIVVNSERVSDENIIMMSKDIAKKIVTDLSYPGQIKVVVIR
                     ETRSIEYAK"
     gene            complement(1953..2130)
                     /gene="ssrS"
                     /locus_tag="PKN85_03785"
     ncRNA           complement(1953..2130)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKN85_03785"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2408..2662)
                     /gene="zapB"
                     /locus_tag="PKN85_03790"
     CDS             complement(2408..2662)
                     /gene="zapB"
                     /locus_tag="PKN85_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HNT43556.1"
                     /translation="MEIFSINKEVINVNRIEELEEKIDRLIEGYRVMKEENERLKAEI
                     ESFQSSDRDLQGKISEIRGERELLIEKVQKILEKVERVEI"
     gene            complement(2669..3788)
                     /gene="prfB"
                     /locus_tag="PKN85_03795"
     CDS             complement(join(2669..3718,3720..3788))
                     /gene="prfB"
                     /locus_tag="PKN85_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156919602.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNT43557.1"
                     /translation="MIDELRGKIDELQERIRSLRGYLDLPQLNEKLTEFDRQISEAAF
                     WDDQEKAQRILRDRSRVDEEIKNWQKKEEELEEILILSDFIRETEDSKDMEELVGRVG
                     SLEESLGSYEIERMLSGENDENNAIIFINSGAGGTEAQDWAEMLFRMYLKWAEKKGFE
                     TQVVDLLPGEEAGVKNVTFVVSGKHAYGYLKCENGIHRLVRVSPYDANNRRHTSFASV
                     YAYPEISEDIQVDIREEDLRIDTYRSSGPGGQHVNRTDSAVRITHLPTGIVVQCQNER
                     SQHKNKAMAMAILKSRIYELEKKKQEEKMDSLNKEKKDIAWGSQIRSYVLHPYRLIKD
                     HRTKHEEHNADAVLDGDIDAFIKEYLLYLTFDQKRKDN"
     gene            complement(3816..>4619)
                     /locus_tag="PKN85_03800"
     CDS             complement(3816..>4619)
                     /locus_tag="PKN85_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNT43558.1"
                     /translation="LVNPQAVDMVIFRENTEDLYRGIEWKGGTAEARTVIEFLRNVMG
                     VEVDPDSGIGIKPMSRAGTQRFVRWAVDWAVKARRRAITIVHKGNIMKYTEGAFREWA
                     YETAREFDDVSAEGTEGKIPINDRIADNMFMQVIARPGEYDVLLCPNLNGDYLSDACA
                     ALIGGLGVAPGANIGDDVAIFEPTHGSAPKYAGQDMVNPTSFILSGAMLFDHIGMAAT
                     GEAIRRAVEETIQAGVMTYDLARQVRDGKAVKCSEYGRAVVERLSGSGV"
BASE COUNT          858 a         1428 c         1078 g         1255 t
ORIGIN      
        1 aggaagggca ccacctcttt cttcttccac acatggttgc ccgtagttat ggcatcgaca
       61 ccgagggaga gcatctccac cgccgtcctg gtggttatgc ctatcccacc cgcgaggttc
      121 tcgccattta ttacaacaaa atcagcgcgc gtctcctcca ggtaacggcc aacggccttc
      181 cttcccggct tcccgatcac gtcgcccagg aagagaactg taaaccggtc attttgcata
      241 ttctatcgaa cgtgtctccc tgatcacgac gaccttgatc tggcctggat aggacagatc
      301 ggtcacgatc ttcttcgcga tgtccttcga catcattatg atgttctcgt cgcttaccct
      361 ctcgctgttc acgacgatgc gtatctctct gcccgcctgt atggcgtagg acttttcaac
      421 accctggaag gtattggcga tcttttccag ttcctggagc ctcttgatat aggtttccag
      481 catctccctg cgggctcccg gcctcgcgcc ggaaagggcg tcggcggcct gaacgatcac
      541 atcgaggagg cttttgacct caacgtcctc gtggtgggcg agaatggaat ggatgatctc
      601 gtcgctctcc ccgtactttc ttgccaggtc agcccctatc gtcgcatggg agccctcgat
      661 ctcgtggtcg acggcctttc cgatatcgtg aaggagcccc gccctcttcg cctccttgac
      721 gctcaccttc agttccgagg caatggcccc gcagatgaag gccacctcca aagagtgctg
      781 gtagatgttc tgggcgtaac tgctgcggaa cttgagtctt cccagaagtt tgacgagctc
      841 ggggtgaacg ttgtggatcc cgaggtcgaa caccgcctgt tcgccggcct ccttgatctt
      901 ctcctccatc tcctcggcaa ccttggagac gatctcctcg atccgggcgg ggtgtatcct
      961 gccgtcgctt atgagctttg cgatggcgat ccttgcgacc tccctccgta cggggttgaa
     1021 gcaggaaaga atgacctctt cgggggtgtc gtcgatgatg atatccacgc ccgtcgcggc
     1081 ctccagggcc ctgatgttac gcccctcacg acctatgatc ctgcccttca tctcctcgtt
     1141 cggcagggga accacggaaa cggtatcctc gccgacgtat tcaccggcat acctctggat
     1201 ggcaaaagct atgatctcct tcgccttctt gtccgccttc tccctggctt cctcttcgat
     1261 ctttttgatc atcttcatcg attcgtagcg ggcctcttcc tccatggccg tcacgagcat
     1321 cttctttgcc tcttcgcccg tcatgcccga tatcttctcg agctgctcct tcgaccgggc
     1381 aaccagggtc tcctgctctc gtatcctgcc ttccaggtga ttgtacctgt tcgacatctc
     1441 gcggtccttc cgcgacagtt cttcctcttt cttttcgagg gcatcgagtt ttttatctat
     1501 attgagctct ttctgggaaa gcctgcgctc ggtgttctgc atctcgacct tgcgcgcctt
     1561 cacctcctgt tctatctcat tgcgggcctg aaggacaatg tccttcgcct gaatggaagc
     1621 ctcccgcatg acaacgtcgg cctctttctt cgcgtcgtcg aggatcttct ttccgatctt
     1681 gtcgtattcg cctacttttt tctgatggat aagcttggta agaaaaaagc cgatgaagag
     1741 cgctataaca aaagcaataa cggctatgac gattgtattc accacgaccc cctaatatat
     1801 ctcaatatta ttaagggggt gcaatcaaaa ggaaagaaag ggcattaaca atatagataa
     1861 aacatcttaa atctttatat tgaatacccc tccatgacgg ctcgaagttt atttatgtca
     1921 aacctctgtt tccttgagca ttacccctta aaattagccc cgcagatgcc gtgtagacga
     1981 tatgtcttga acctcgtgtt cacgtgggtg tcgttatact gcttcaggct tctcactcga
     2041 ggtgggcatg cacaccgcag cagggaacgc ctcccggtgt tgaggtgttg gctcacagcc
     2101 taccgcctat cacgtacacc gcagggaaca tctatatttt caatatcttc gaactttttg
     2161 ataagtcgaa gagtccttcc gtccacgctc tctacctgtt gcttcatctc caggtattcg
     2221 tcagccagtt ccatcattgc catgatcacc gcgttcaggg tattgactat accgtaccgc
     2281 gtcacgacac ggtcgatctt gtcgcctaca aagcgggcat cgcgctttat cctctcatca
     2341 tcctccccga cgaaagtaaa aggctgtttc aggatggtca cttcaacctt tttctccatg
     2401 gaatccccta aatctctact ctttctactt tttcgagtat tttctggacc ttttcgatga
     2461 gtaattcccg ctcgccccga atctccgaga tcttcccctg cagatcccta tcgctgctct
     2521 gaaacgactc aatctcggcc ttcaacctct cgttctcttc cttcataacg cggtagcctt
     2581 cgatgagccg gtcgatcttt tcttccagtt cttctatgcg gtttacgtta attacctctt
     2641 tatttatact gaaaatttcc atatctagct aattatcctt ccttttctga tcgaaagtca
     2701 agtacaaaag gtattcctta ataaaagcat cgatatcccc gtcaagcacc gcgtcggcat
     2761 tatgttcctc gtgtttcgtc ctgtgatcct tgatgagcct gtacggatga agaacatagg
     2821 agcgtatctg acttccccag gctatgtcct tcttttcctt gttcagggaa tccatcttct
     2881 cttcctgttt tttcttctcc agttcataga tcctggactt caggatggcc atggccatgg
     2941 ccttgttctt gtgctgggac cgctcgttct ggcattgtac gacgatgccc gtggggagat
     3001 gggtgatcct caccgccgaa tcggtcctgt tgacgtgctg gccgccggga ccactcgacc
     3061 tgtacgtatc tatacgcagg tcctcttccc tgatgtcgac ctgtatgtcc tccgatatct
     3121 cgggataggc gtaaacggag gcaaaggagg tgtgccgccg gttattggcg tcgtagggcg
     3181 agaccctgac gagacggtgg atgccattct cgcacttgag atagccgtat gcgtgtttac
     3241 ccgacaccac gaaggtgaca ttcttgacgc ccgcctcttc accgggcagc aggtcgacga
     3301 cctgggtttc aaatcccttc ttttccgccc acttcaggta catcctgaag agcatctccg
     3361 cccaatcctg ggcttcggtc ccccctgccc cggagttgat gaagatgatg gcgttgtttt
     3421 cgtcgttctc gccggagagc atcctctcga tctcgtagga ccccagagat tcttcgaggg
     3481 aaccgacccg gccgacaagc tcctccatat ccttcgaatc ttccgtttcc cgaatgaaat
     3541 cgctgagtat gagaatctct tcgagctctt cttccttttt ctgccagttc tttatctctt
     3601 catcgacccg cgaacggtcc ctcaggatcc tctgggcctt ttcctggtcg tcccagaacg
     3661 cggcctcgga gatctgcctg tcgaattccg tcagcttttc gttgagttga ggcaggtcaa
     3721 agataacctc tgagtgagcg gatgcgctcc tgaagctcgt ctatctttcc cctgagttcg
     3781 tctatcatat ctcttacctt ccctgtgtct ctatattata ccccggagcc ggacagtctc
     3841 tccacgaccg ccctgccgta ttcggaacac ttcacagcct ttccatcccg gacctgacgc
     3901 gccaggtcgt acgtcatgac ccccgcctgt atcgtctctt caacggcacg gcggatcgcc
     3961 tcccccgtcg ccgccatccc tatgtggtcg aagagcatcg cccccgacag gataaaggac
     4021 gtggggttca ccatgtcctg acccgcgtac ttcggagcgc tcccgtgtgt gggctcgaat
     4081 atcgccacat cgtcgccgat attcgccccc ggagcgacac cgaggccccc gatcagggct
     4141 gcgcaggcat cggacaggta gtcgccgttg aggttcgggc acagcaggac atcatactct
     4201 ccgggcctcg cgatgacctg catgaacatg ttgtccgcga tcctgtcgtt gatgggtatc
     4261 ttgccctcgg taccctcggc cgagacatca tcgaattccc gtgccgtctc gtatgcccac
     4321 tcacggaacg cgccctcggt gtatttcatg atgttgccct tgtgtacgat ggtgatggcc
     4381 ctccgacgcg ccttgaccgc ccagtcgacg gcccatctga cgaaccgctg agtgccggcc
     4441 cggctcatgg gttttatccc aataccgctg tcgggatcca cctccacacc catcacgttg
     4501 cgcaggaact cgatcacggt cctggcctcc gccgttcccc ctttccattc tatgccccgg
     4561 tagaggtcct cggtattctc cctgaagatc accatgtcca ccgcctgagg gttcacaag
//
LOCUS       DAOQTR010000110         4595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20901_length_4595_cov_3.603524, whole genome shotgun sequence.
ACCESSION   DAOQTR010000110 DAOQTR010000000
VERSION     DAOQTR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20901_length_4595_cov_3.603524"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKN85_03805"
     CDS             <1..533
                     /locus_tag="PKN85_03805"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43559.1"
                     /translation="FLGPNKVALEKGEILKGFAMPAFGEGTGSAYIKHTRREAMDLPM
                     LGVAARVTIRVEGSEVGCRDAMCTIDTISNILKRFEDEGLVCEDARIAMGVVAPRPMR
                     AKKAEEALKGKVISEKLLGEIGEIAASEAQPRDSIRGEAWYRRDMIQVLTGRAILRAV
                     DRVIRPDDVIWPQRLW"
     gene            544..1011
                     /locus_tag="PKN85_03810"
     CDS             544..1011
                     /locus_tag="PKN85_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT43560.1"
                     /translation="MKKEITFTLNNEKIMVEADPKWTLLYLLRDVLEMTGTKEGCGYG
                     ECGACTVIIDGKAVNSCLYPVMEAEGRNIVTIEGLMSRDGELDPLQKAFIDEGAVQCG
                     FCIPGMIMSAKALLDGKKDPTDEDIKESIEGNLCRCTGYVKIIDAIKSVAGGR"
     gene            1014..3299
                     /locus_tag="PKN85_03815"
     CDS             1014..3299
                     /locus_tag="PKN85_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNT43561.1"
                     /translation="MDGLKFVGQRIPKKDAPLKATGEALYIQDIKVPGMLYGKILYSK
                     YPHARLLKVDAAKALALPGVKAVLTGADVPNNFKFGFLKDNPPLKSGKVLSTRDEVAA
                     VAAISPEIAEQALSLIEVEYEELPGIFDPLEAMKEGAALIHEEFKSNVLKMPWKFVHG
                     DVEAARKESAFIAEDSFSTQWVTHCCLGTSGCIASFDVGNNLTMYSNTQIPSLAQNDY
                     LEALKTFGLKNRRVRIIQAVIGGGFGSKLDTYAYEYIAILLALRTRKPVKIVFTREEE
                     FLATSPRQCTITKISQGCDKEGRLTFREIEMVLDNGAYTSWGATTPSVMMVPISSLYK
                     VPNVKYTAKCVYTNNTYCQAFRGYGNPQATFAIESCLDQLAEKAGIDPLDIRRINANE
                     PGEVTPQNFRITSCGMKDCIEEVNRRLGWKDKKGKTGDRGVGMASLIHVGGAARVYKS
                     DGCGTIIKVDDNGKVDVITGSSEIGQGSDTIISQIVAEVLGVSIDDINVINNDTDVCP
                     WDVGVHASRTTFVAGNSALGAAKKIKAQIMEIAAKNLGTEPEELEMRDGVIFSMKDKE
                     KSIPLSKVLRKAHYTLGGRMLVAEHFYDPPNENFDQTFRGNLSVSYAYGAHGVEVEVD
                     RQTGQVKILNYIAAHDVGKAINPMLLEGQIYGGGLQGIGYALGERMVYDKGYLKNGNF
                     LDYKMPTVRDVPPVQAVIVESDERDGPFGAKGIGEPGLVPTAPAIANAVYDAVGVRIK
                     DLPITPEKVLRALKEKAGERS"
     gene            3310..3729
                     /locus_tag="PKN85_03820"
     CDS             3310..3729
                     /locus_tag="PKN85_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNT43562.1"
                     /translation="MKIERIDHICFAVSNLEETKRVYREDFGLVPSCEYTAESEKIRV
                     SRYYIGEVAVEFMESTAPDGEVAKFIEKRGEGVFLISYKVDDLTKAMNELKEKNVELI
                     DKKPRELFGTRYAFVHHPNKLHGVLTELLEGDFDINK"
     gene            3780..>4595
                     /locus_tag="PKN85_03825"
     CDS             3780..>4595
                     /locus_tag="PKN85_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNT43563.1"
                     /translation="MKVLVLGGTGGMGQGVARDLIKQDRIEKVVLGDINVDPARVQEK
                     LRASAKVSLAKIDVNDHAGMVNEIKDADVVVNCAGPFYKTAVAVARAAVEARVNYIDI
                     CDDYEAAQILFASDIDAAAKAAGITVLTGMGSDPGTNNVLVKWYANRLDRVDEIFLYW
                     VVSIAELAGAAWDHSLHMVLGKIPQYIDGQLVEVDGGGGEEIEHFLEPLGECLISYVG
                     HPQPITIPRYIEGVKTVVIKGALIPLWVDKLIKEQKGMGLLGSEPVEVRGGKVV"
BASE COUNT         1098 a         1207 c         1492 g          798 t
ORIGIN      
        1 tcttcctcgg accgaacaag gtggccctcg agaagggcga gatactgaag ggcttcgcca
       61 tgcccgcctt tggcgagggg accggttcgg cgtacataaa gcacacgagg cgggaagcca
      121 tggacctgcc gatgctcggg gtcgccgcaa gggtgaccat tagggtcgaa gggagcgaag
      181 tgggctgcag ggacgccatg tgcacgatcg acactatctc gaatattctg aaaaggtttg
      241 aggatgaagg gcttgtttgc gaagatgcgc gcatagccat gggcgttgtg gccccgaggc
      301 cgatgagggc gaagaaagcc gaggaggcct tgaagggaaa ggtgatatcg gagaagctcc
      361 tgggagagat aggagagatc gccgcgtccg aggcgcagcc cagggacagc atacggggag
      421 aggcatggta tcgccgggac atgatacagg tcctcaccgg ccgtgccatc ctcagggcgg
      481 tcgaccgtgt cataaggccg gacgacgtga tatggccgca acgactctgg tagggggtgg
      541 gtcatgaaga aagagatcac gttcacattg aacaacgaaa aaataatggt cgaagcggat
      601 cccaagtgga cgcttcttta cttgctgcga gacgtgctcg agatgaccgg aacaaaagaa
      661 gggtgcggtt acggagagtg cggggcctgc acggtgatca tcgacggcaa ggcggtcaat
      721 tcgtgtctct atcccgtcat ggaggccgaa ggcaggaaca tcgtgacgat agaagggctg
      781 atgagcaggg atggggaact cgaccctctc cagaaggctt tcatcgacga aggcgcggtt
      841 caatgcgggt tctgcatacc cggcatgata atgtccgcga aggccctcct ggacgggaag
      901 aaggacccga cggatgagga catcaaggag agcatcgaag gtaatctctg caggtgcacc
      961 ggctacgtga agatcattga tgccatcaag tccgttgcgg gtgggaggtg accatggacg
     1021 gcctgaaatt cgttggacaa aggataccga agaaggacgc gccgctcaag gccacgggag
     1081 aggcgctcta catacaggac atcaaggtcc cgggcatgct ctacggcaag atcctgtaca
     1141 gcaaatatcc tcacgcaagg ctgctgaagg tggatgccgc gaaggcgctg gcactccccg
     1201 gggtcaaggc ggtgcttacc ggtgccgatg tgccgaacaa cttcaagttc ggttttctga
     1261 aggacaatcc tcccctcaag tcgggaaagg tcttgtcgac gcgtgacgag gtagcggccg
     1321 tcgccgccat aagtcccgag atagcggagc aggccctcag cctcatcgag gtggaatacg
     1381 aggaactgcc ggggatcttc gatcccctgg aggccatgaa ggaaggggcg gccctcatcc
     1441 atgaagagtt caagtcgaac gtcctcaaga tgccctggaa gttcgttcat ggcgatgtcg
     1501 aggcggcaag gaaggaatcg gccttcatcg cagaggacag tttcagcacc cagtgggtca
     1561 cgcactgctg cctcggcacg agcggctgca tagcctcctt cgacgtgggc aacaatctca
     1621 cgatgtacag caacacgcag atcccttccc tcgctcagaa cgactacctg gaagccctga
     1681 agaccttcgg tctcaagaac aggcgggtga ggatcatcca ggccgttata gggggaggtt
     1741 tcggaagcaa actcgacacg tatgcctacg agtatatcgc gatcctcctc gcgctgagga
     1801 cgcgtaagcc cgtcaagatc gttttcaccc gggaggagga gtttctggcc acctcgccga
     1861 ggcagtgcac catcacgaag atatcccagg gttgcgacaa ggaaggccgg cttaccttca
     1921 gggagataga gatggtcctc gacaacggcg cctacacctc ctggggggcc acgacacctt
     1981 ccgtcatgat ggtccccatc tcgtccctct acaaggtccc caatgtcaaa tacaccgcga
     2041 agtgcgtcta taccaacaat acctactgcc aggccttcag gggctacgga aatccccagg
     2101 ccacttttgc catcgagtcc tgcctcgatc agctcgctga gaaggccggg atcgacccgc
     2161 tcgatatccg ccgaatcaac gcgaacgaac cgggtgaagt gacaccccag aacttcagga
     2221 tcacctcctg cggcatgaag gactgcatag aggaggtgaa caggaggctt ggctggaagg
     2281 acaagaaggg aaaaacaggt gaccgcgggg tcggcatggc ctccctcatc catgtcggcg
     2341 gggccgccag ggtgtacaag tccgatggct gtggcaccat catcaaggtg gacgacaacg
     2401 gcaaggtgga cgtcatcaca ggctcctcgg agataggcca gggatcggac accatcattt
     2461 cccagatcgt cgcagaggtg ctcggtgtca gtatagacga catcaacgtc atcaataacg
     2521 acaccgatgt ctgtccgtgg gacgtcgggg tgcatgcgag caggacgacc tttgtcgccg
     2581 gcaattccgc cctgggcgcc gcgaagaaga taaaggccca gatcatggag atcgcggcaa
     2641 agaatctcgg cacggagccc gaggaactgg agatgaggga cggcgtcatc ttctcgatga
     2701 aggacaagga gaagagcatc cctctctcga aggtgttgcg caaggcgcac tataccctcg
     2761 gcggcaggat gctcgtggcg gagcacttct acgatccgcc caacgagaac ttcgaccaga
     2821 ccttcagggg caacctttcc gtctcgtacg cctacggggc gcatggcgtc gaggtggagg
     2881 tggacaggca gaccgggcag gtgaagatcc tcaactacat cgccgcccat gacgtgggca
     2941 aggccattaa ccccatgctg ctcgaaggtc agatctacgg gggcgggctt cagggtatcg
     3001 gctacgccct gggcgaaagg atggtctacg ataaaggata cctgaagaac ggaaactttc
     3061 tagactacaa gatgcccacc gttcgggacg tgcctcccgt tcaggcggtg atcgtggagt
     3121 ccgatgagcg agacgggccc ttcggtgcga agggtattgg cgagccgggc ctggtcccga
     3181 cggcgcccgc gatcgccaac gccgtttacg atgccgtggg ggtcaggata aaggaccttc
     3241 cgatcacccc cgagaaggtt ttgagggccc tgaaggagaa ggcaggggaa agatcatagg
     3301 ggggcgcaca tgaagatcga aaggatcgac cacatatgct tcgcggtcag taacctggag
     3361 gagacgaagc gcgtgtacag agaggacttc gggcttgtac cgtcctgcga gtacaccgcg
     3421 gaatcggaaa agataagggt gtcgcgctac tacatcgggg aggtcgccgt cgagttcatg
     3481 gaatcgacgg cacccgacgg ggaggtcgcg aaattcatcg agaaaagggg agagggcgtc
     3541 ttcctcatat cctacaaggt cgacgatctc acaaaggcga tgaacgaatt gaaggagaag
     3601 aacgtcgaac tcatcgataa gaaaccccgg gagcttttcg gcacgcggta cgccttcgtg
     3661 catcacccca acaagctcca cggtgtgctt acggagcttc tggaaggtga tttcgacatt
     3721 aataagtgat aactggagtg cgttttgggg cagagagtca aggatcataa ggagggtgaa
     3781 tgaaggtact ggtgctggga ggaacgggag gcatgggcca gggggtcgcg cgggacctca
     3841 tcaagcagga ccggatagag aaggtggtgc tgggggacat caatgtagac cccgccaggg
     3901 tgcaggagaa actgcgggcc agtgcgaaag tgtccctggc gaagatcgat gtgaacgacc
     3961 acgccggaat ggtgaatgag atcaaggacg ccgatgttgt tgtcaactgc gcggggccct
     4021 tttacaagac cgccgtggcg gtcgccaggg cggccgttga ggcaagggtc aactacatag
     4081 acatatgcga cgactacgag gcggcacaga tcctcttcgc atcggatatc gacgccgcgg
     4141 cgaaagcggc cggcataacc gtcttgaccg ggatgggatc agaccccggg acgaacaacg
     4201 tgctcgtcaa gtggtatgcg aacaggctcg accgcgttga cgagatattc ctctactggg
     4261 tggtgagcat cgcggagctg gcgggtgccg cctgggacca cagcctccac atggtcctcg
     4321 gcaagatacc tcagtacatc gacggtcagc tggtcgaggt ggacggaggc ggcggtgaag
     4381 agatcgagca tttcctggaa cctctggggg aatgcctgat aagctatgtc ggccatcccc
     4441 aacccatcac gataccccgt tacatagaag gggtgaagac ggtggtcatc aagggggccc
     4501 tcatccccct ctgggtggac aagctcatca aggagcagaa gggtatggga ctcctcggca
     4561 gcgaacctgt cgaggtcagg ggagggaagg ttgta
//
LOCUS       DAOQTR010000111         4587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_20948_length_4587_cov_2.947264, whole genome shotgun sequence.
ACCESSION   DAOQTR010000111 DAOQTR010000000
VERSION     DAOQTR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20948_length_4587_cov_2.947264"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1026)
                     /locus_tag="PKN85_03830"
     CDS             complement(13..1026)
                     /locus_tag="PKN85_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43564.1"
                     /translation="MSTVLNISGDEHITIDTFQPGDALGVGDLFRKVYGEDYPVKLVY
                     DPEGLVKAFEKHENIPAVARSSKGIVVGYVSIFRSAPVETVYEGGQGLVLADYREHGT
                     FRELIRYYSEVLAPAYGADAVFGEAVCNHIYSQKAWAMHGAIETALEVDLMPGAVYAK
                     EGTTGRVATLHMTRMYRPMEHTVYAPRQYEEAMRFIYDGFTSGARVAKATDESAEREP
                     TSIESSHFEFAHVTRMTVRQAGQDFPAVFEEYERDALQKGMVVIQVWLKLSWPFIAGL
                     TDWLSGRGYFFGGILPRWFGEDGLLMQKIAVRPNWEGIMLYSDRAKDILAAVREDWQR
                     VTT"
     gene            complement(1065..1991)
                     /locus_tag="PKN85_03835"
     CDS             complement(1065..1991)
                     /locus_tag="PKN85_03835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT43565.1"
                     /translation="MEADPTLSIVIITKDTRELLEGLFDSIEKDTSLTSLGREIIVID
                     NASTDGTEKFVKSACPHARYIRNDRNAGFAVAVNQGARAAAGRYILLLNSDTRLIEGE
                     VARMVATAEDIPDLGLMGPQLVYEDLSLQRSVAAVPGLTGEVIPGSGRVSQPASDGNT
                     GPARDVESLIGAAIMIRKAAFDDLHGFDERFFFFLEETDFCVRARQKGYRVVFFPGAR
                     VIHLQGRTVRKTWVKGRMEYNISLRKFMKKHHGRLYLTAFDAVRLAKSLAFVIAVPLS
                     LFGSRMRLRYSYYLNLVVWYMRACPDDAGLRG"
     gene            complement(1999..3792)
                     /locus_tag="PKN85_03840"
     CDS             complement(1999..3792)
                     /locus_tag="PKN85_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT43566.1"
                     /translation="MHSREEVVIGNQAIARGLVEAGLEIAAAYPGTPSSEILPGVVYF
                     RDMEGSKIHTEWSTNELCAFEVAFGAAAYGGARAACFMKQVGLNVAFPALMRSREKSI
                     EGGLVIVSCDDPGPQSSQTEQDTRLIATLFGIPVFDPSSPAEAADVAYHALNWSFENK
                     VPVIIRSTHRVSHARESVPLYGPGTRRVAFREGHVAGRGRKLGIVASGMSCPLVYDVM
                     EELGASEEVSLYKVLKVYPVDEALTGFVDSMERVLVVEETDQVIEALIGDAKKVMGRR
                     DGTVPSAGEITYDIVRDILSRVLAGAGTDRRPFVPDGGIEEALSHVAVVPRPPKLCPG
                     CPHRASFFAMQYLWPDAIYPGDIGCYTLGISQGAVDTCLDMGAGVTLAEGFYDAFNQD
                     GKLVPILASIGDSTFLHACMAPLYDAVRNRKRFILVIMDNSTTAMTGMQPTPQSGVTV
                     DGTRTRSVAIEDIVRGLGVNYVRVVDPYDVPAMIGTINEAFGRVREEALPAVIIARRE
                     CLLLVKGAAKPSAYPADIERDCIGCKSCLKTFDCPAMSFDEVSKKIRIDDSLCTNCGI
                     CYYACPVQPQGKALKAEKKAGRPGAAAPGNP"
     gene            complement(3814..4401)
                     /locus_tag="PKN85_03845"
     CDS             complement(3814..4401)
                     /locus_tag="PKN85_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT43567.1"
                     /translation="MRHEIICAGIGGRGVLLASTILIEAAIGSGLKAVASDEYGMSQR
                     GGSVVSLVKVGDFASPFIGRENGGILLAFEESEFYKALLFLKKGGLALVNTRAAELPP
                     SIAGLLEKREITCRLIDADGIARQTGMFQSANMALLGFFAAFGIAPFTKEVLEETIRS
                     RVPEKFLAKNLEVFGKGFDAAMKMGNAGDRRTDMV"
     gene            complement(4398..>4587)
                     /locus_tag="PKN85_03850"
     CDS             complement(4398..>4587)
                     /locus_tag="PKN85_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43568.1"
                     /translation="VNSLYNGSSFVLLVMVSEREDDLRRILRGYGVSGVHSISNIDEI
                     GRFRDTKEPVVALYRGMI"
BASE COUNT          877 a         1473 c         1356 g          881 t
ORIGIN      
        1 acctcaaggg atttacgttg tcaccctttg ccagtcttcg cgcactgccg cgagaatgtc
       61 cttcgccctg tcggagtaca gcattatccc ctcccagttc ggacggacgg cgatcttctg
      121 catgaggagc ccgtcttcgc cgaaccaccg ggggagaatg cccccgaaga agtatcccct
      181 cccgctgagc cagtccgtca gaccggcgat aaagggccag gagagcttga gccacacctg
      241 gatgacgacc atgcccttct gaagggcatc gcgttcatac tcctcgaaaa cggcggggaa
      301 gtcctgcccg gcctggcgca cggtcatgcg cgtgacgtgg gcaaattcga aatggctgct
      361 ttcgatggat gtcggctccc tttccgcgga ctcgtccgtc gccttcgcga cacgggcacc
      421 gctcgtgaac ccgtcataga tgaaccgcat ggcctcctcg tattgccggg gcgcgtagac
      481 ggtgtgttcc atgggccggt acatccgcgt catgtgcagg gtggccacgc ggcccgttgt
      541 gccctccttc gcgtagaccg cgccgggcat gaggtccacc tcgagggcgg tctcgatggc
      601 cccgtgcatt gcccaggcct tctgggaata gatatggttg caaacggcct cgccgaagac
      661 ggcatcggca ccgtacgcag gcgccagtac ctcggagtaa tagcggatca gctctcggaa
      721 ggtgccgtgc tcgcggtagt ccgcaaggac gagaccctgt cccccttcgt aaaccgtttc
      781 gaccggggcg gacctgaaga tgctcacgta ccccacgacg atgcccttcg aagaacgggc
      841 gacggcgggg atgttttcat gtttctcaaa ggccttgacg agcccctcgg gatcgtagac
      901 aagctttacc gggtaatcct ccccatagac cttgcggaaa aggtccccca cgcccaaggc
      961 gtcaccgggc tgaaaggtgt caatggtgat atgttcgtct cccgagatat tgagtacggt
     1021 cgacatgcgt tccttacctc ctattcgtcc cgcaccggcg tccgtcagcc gcgaagtccc
     1081 gcgtcatcgg ggcaggcgcg catgtaccat accacaagat tgaggtaata ggaatagcgg
     1141 agccgcatcc gggagccgaa aagggaaagg gggacagcaa tgacaaaggc cagggatttt
     1201 gccagcctga cggcatcgaa ggccgtgagg tacaggcgtc catgatgctt tttcatgaac
     1261 ttcctcaggg agatattgta ttccatgcga cccttcaccc aggtcttcct gaccgtcctt
     1321 ccctggaggt ggatgacccg ggccccgggg aagaagacca cccgataacc cttctgcctc
     1381 gcccggacgc aaaagtccgt ctcctcgaga aagaagaaga agcgctcgtc gaaaccatga
     1441 aggtcgtcga aggcggcctt ccggatcatg atggcggcac cgataagcga ttccacgtcc
     1501 cgggcgggcc ccgtgttccc gtccgacgcc ggttgagaga cgcggcccga accggggatc
     1561 acctctcctg tgaggccggg gacggctgcc accgaccttt gaaggctcag gtcctcgtaa
     1621 acgagctgag gccccatcaa tcccagatcc gggatgtctt cagcggtggc caccatccgc
     1681 gccacctctc cttcgatgag gcgggtgtcg gagttgagga gaaggatata tctgccggcg
     1741 gccgcccgcg ccccctgatt caccgcaaca gcgaatcccg cgttgcggtc gttgcgtata
     1801 taccgggcat ggggacaggc gctcttaaca aatttctccg tgccgtcagt ggacgcgttg
     1861 tcgatgacga tgatctccct gccgagagac gtaagcgagg tgtccttttc gatggaatca
     1921 aagagcccct ccaggagctc cctggtgtct ttcgtgataa tgactatgga aagggtgggg
     1981 tccgcttcca tgacctcttc aggggttgcc cggcgccgcc gcgcccggtc tgcccgcttt
     2041 cttttccgcc ttcagggcct taccctgcgg ctggacggga caggcatagt agcagatccc
     2101 gcagttcgtg cacaggctgt cgtctatccg tatcttcttc gacacctcat cgaaggacat
     2161 cgccgggcag tcgaaggtct taaggcaact cttgcagcct atgcaatccc gctcgatgtc
     2221 cgccgggtag gcggagggct tcgccgcccc ttttacaagg agcaggcact cgcgccgggc
     2281 gatgatgacc gccgggaggg cttcctccct gacgcgcccg aaggcctcgt tgatggtccc
     2341 gatcatcgcg ggaacgtcgt aaggatcgac aacgcgcacg tagttcaccc cgagacccct
     2401 gacgatgtcc tcgatggcga cgcttcgcgt acgcgtcccg tccacggtga cccccgactg
     2461 cggcgtgggc tgcatgcccg tcatggccgt cgtggagttg tccatgatga cgaggatgaa
     2521 cctcttccgg tttctaacgg cgtcatagag aggggccata caggcgtgca gaaacgtcga
     2581 gtctcctatc gacgcaagga tggggacgag cttgccgtcc tggttgaagg cgtcatagaa
     2641 gccttcggcg agggtgaccc cggcgcccat atcgaggcag gtgtcaacgg caccctgtga
     2701 tatgcccagc gtgtagcacc cgatatcgcc gggatagatg gcgtcgggcc agaggtactg
     2761 catcgcgaag aaagacgccc tgtgggggca cccgggacag agcttcgggg gcctggggac
     2821 gacggccaca tgggaaagcg cctcttcgat gcccccgtca ggcacgaatg gacgcctgtc
     2881 cgttccggcc ccggcaagca cccgggacag gatgtccctc acgatatcgt aggtgatctc
     2941 tcccgccgac ggcaccgtgc cgtccctgcg gcccatcacc tttttcgcgt cgccgatgag
     3001 cgcctctatc acctggtccg tttcctcaac cacgagaacg cgttccatgg aatcgacaaa
     3061 ccccgtgagg gcctcgtcga cgggatagac cttgaggacc ttgtaaaggg aaacctcttc
     3121 cgacgcgccc agctcttcca tgacatcgta gacgaggggg caactcatgc ccgacgcgac
     3181 gatgcccagc ttccggcccc ttccggcaac gtgtccttcc ctgaaggcca ccctcctcgt
     3241 ccccgggccg tagaggggaa ccgactcgcg ggcatggcta acccggtggg tggaacggat
     3301 gatgacgggt accttgttct cgaaggacca attgagggcg tggtaggcga catcggccgc
     3361 ctcagccggc gatgacggat cgaaaacggg gatcccgaag agggtcgcta taagccgcgt
     3421 gtcctgctcc gtctgggacg actgcgggcc ggggtcgtca caggagacga tcacgagccc
     3481 tccctcaatg gacttttccc gggacctcat cagtgccggg aacgctacat tgaggcccac
     3541 ctgcttcatg aaacaggccg ctctggcccc gccgtaggcc gcggccccga aggcgacctc
     3601 gaaggcgcag agctcgttcg tcgaccactc ggtgtggatc ttcgatccct ccatgtccct
     3661 gaaatagacg actccgggca gtatctcgga actcggcgtc cccgggtatg ccgccgctat
     3721 ctcaaggcct gcctcgacaa ggcccctcgc gatggcctga ttgcctatta ccacttcttc
     3781 cctgctatgc actgacgatc ctcccataag ctcttaaacc atatccgtcc ggcggtcccc
     3841 cgcgttgccc attttcatcg cggcgtcgaa tcccttcccg aagacttcga gattctttgc
     3901 caaaaacttc tccggcacgc gggacctgat cgtctcttcg agaacctctt tcgtaaaagg
     3961 ggcgatgccg aaggcggcga aaaagccgag aagggccatg ttcgcggact ggaacatgcc
     4021 cgtctgacgg gcaataccgt cggcatcgat gaggcggcag gttatctccc gcttctccag
     4081 aagacccgcg atggacggcg gcagctcagc cgcacgggtg ttgacaaggg caaggccgcc
     4141 cttcttcagg aagaggagcg ctttgtagaa ctcgctctcc tcgaaggcga ggaggatgcc
     4201 gccgttctcc ctgccgatga agggactcgc gaaatcgccc accttcacga gggacacgac
     4261 ggaccctccc ctctggctca taccgtattc atcggatgcc accgccttga gcccgctccc
     4321 gatggcggcc tcgatgagta tcgtggaggc gaggagcaca cccctgcccc cgataccggc
     4381 gcatatgatc tcatgtctca tatcataccc ctgtagagag ccaccacggg ctccttcgtg
     4441 tcccggaacc ggccgatctc gtcgatgttc gagatcgagt gcaccccgct tacgccatag
     4501 ccccgcagta tccttctcag atcgtcctcc ctctcgctca ccatgacgag aagtacgaac
     4561 gaagagccat tgtagaggct gttgacg
//
LOCUS       DAOQTR010000112         4575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21026_length_4575_cov_3.978097, whole genome shotgun sequence.
ACCESSION   DAOQTR010000112 DAOQTR010000000
VERSION     DAOQTR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21026_length_4575_cov_3.978097"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKN85_03855"
     CDS             <1..570
                     /locus_tag="PKN85_03855"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNT43569.1"
                     /translation="SNTIAGNISAGAGVPPSAVDYILGICKTYTTRVGEGPFPTELPG
                     PEGELMRKIGDEYGATTGRPRRCGWFDAVLVRRAVKLSGINGLSLMKLDVLDSFDTIK
                     ICTHYKIGKRLYEEPPMALQDFYRCEPQYEEMPGWKTSIKDARRLEDLPEQTLRYIRR
                     IEELVGVPAAIVSTGPDRESTIILRNPFA"
     gene            701..1396
                     /locus_tag="PKN85_03860"
     CDS             701..1396
                     /locus_tag="PKN85_03860"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HNT43570.1"
                     /translation="MATNLDITQTVSMLQYLQNGRKRADNFIVNTDATGFQDVLLRSL
                     TTGRAAEFSSAQAGPGTSSTSAVDDKETKFRKCLDFVLQKEGSRLVKEDGERGASRYG
                     ILQSTARAFGYKGSIRNITGEQVEGIYRKLWEASGSSSLPYPLCVVHFDTYVNSPAAA
                     RKILQRSQGNIGDYLAMREQRYVRLASAKPQVYGKYLKGWKNRINSLRTVVAEFERDT
                     MYARNGLTTVRKG"
     gene            1545..1754
                     /gene="rpsU"
                     /locus_tag="PKN85_03865"
     CDS             1545..1754
                     /gene="rpsU"
                     /locus_tag="PKN85_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNT43571.1"
                     /translation="MPAVIIREGESFESALKRFKKQCEKTGILSEIKKREHYEKPSVK
                     RKKKILAAKKRALKKLKRTMDKGLY"
     gene            1761..2207
                     /locus_tag="PKN85_03870"
     CDS             1761..2207
                     /locus_tag="PKN85_03870"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNT43572.1"
                     /translation="MDYKSKIEEGLKEALKKRDNIRVSVLRMLLASIKNKEVEKIRPL
                     SDEEFFGLVKTSIKQHNDSIESFRKGGRLELAEKEEKELEILKEFQPSQLSEEEILRE
                     IEEAVAAVGATGRKEMGKVIKFLMDKFPGRIDGKVLSAMVLKRLES"
     gene            2229..4520
                     /locus_tag="PKN85_03875"
     CDS             2229..4520
                     /locus_tag="PKN85_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNT43573.1"
                     /translation="MIDKTLSYLDYLKLRDIILQFSATPFSKDALGNLRPLDDIEEIA
                     RRHDRIEAVLDVIKWDGRVPFSDVPDITGVLARTAIRDSVLEPSEFLSLTNFLRACED
                     VGGFLSKVFKKSAFTDELVAGIDRLTALARRIARSVNSEGYLEDSASYELSRIRADLF
                     MNRERIRKQLERIMGREAMRPIIQDDYISLRNNRYVIPLKPNFNEAVQGIVHDYSHSL
                     KTSFVEPVECVELNNSINILINEEKEEEKKVLRELTDFTRGFREALERNVRCLAELDF
                     FHAMALFCHEFRCVRPEVTLSGSLEVKKAVNPFIAMSRKSEAVPIDIVMKESEKVMII
                     SGPNAGGKTAALKTIGLLSLMAKTGLFIPATERPVVPLFSNVFALIGDEQDISLELSS
                     FTAHMYAIKDLYLNAGGDELVLIDEIGGNTEPQEASALAMGVIDAFVEKGCRVVVTTH
                     LNLIKAYGYMKDYAMNVATSFDTTNMRPLYQLIYGTAGYSNAINVARKIDVPQVIIEK
                     SYTYLNEQEFMLNDLITSLEEEKAKTARERGELAKIKEEARERLKVIRERKDDYLRKW
                     EDKARERLLEVEVEVEEIKKEVAKKERASITKSKDKIRSLKERLRGPAKEVGEDIRIG
                     DYVLVKTLGSKGYVVDIDKEGSVFEVAVGNVRTKLKRMFIEKAIEGPKRVSRDRSEIN
                     VEKTEQRDLKIIGMRVEEALKTVDTFLDRAVVEGTSQVRIVHGVGTGRLMQAVRDHLA
                     ETSYVRAFHADERNAGVTIVEFA"
BASE COUNT         1200 a         1169 c         1351 g          855 t
ORIGIN      
        1 tccaatacca tcgccgggaa catctcggca ggagccggcg tgcctccgag cgccgtcgac
       61 tacatcctgg ggatatgcaa gacatatacg acccgcgtcg gtgagggacc cttcccaacg
      121 gagctgcccg gtcccgaggg agagctcatg aggaagatcg gggacgaata cggggctacc
      181 acgggcaggc cgaggcggtg cggatggttc gacgccgtcc tggtgagaag ggccgtcaag
      241 ctgagcggca taaacggtct ttccctgatg aagctcgatg tgctggactc attcgacacg
      301 atcaagatat gcactcatta caagatcggc aaacggctct acgaggagcc gcccatggcc
      361 cttcaggatt tctaccggtg cgaacctcaa tacgaggaga tgccggggtg gaagacgtcg
      421 ataaaagatg cccggaggct cgaagacctg ccggagcaaa ccctgagata cataagacgc
      481 atagaggagc ttgtcggtgt tcctgccgcc atcgtatcca cggggcccga cagggaaagc
      541 accatcatcc tcaggaatcc cttcgcctga gccggcgggg aacgctcgct gtcataaatc
      601 cgacgccatg gccggccaac ggaagcgtct gtcgaggggt ctcctgtgta acccgtcaga
      661 atctaagagg tggcatagtt gttgcaaaaa attcaccact atggctacca atctcgatat
      721 cacacagaca gtatcgatgc tgcagtacct ccagaacggt cgtaagaggg cggacaactt
      781 catcgtcaat accgatgcca cgggctttca ggacgtcctg cttcgttccc tgacgacggg
      841 ccgggctgcc gagttcagtt ccgctcaggc gggtccgggc acgtcttcca cgtcggccgt
      901 cgacgacaag gaaaccaaat tccggaagtg tctcgacttt gtccttcaga aggaaggaag
      961 tcggctcgtc aaagaggatg gggaacgggg tgcgtcccgt tacggcatcc tgcagtccac
     1021 cgcgcgggcc tttgggtaca agggaagcat caggaacatc accggggagc aggtggaagg
     1081 gatctacagg aagctgtggg aggcatcggg ttcatcttca ctgccctatc ctctgtgcgt
     1141 cgttcacttt gacacctatg tcaacagtcc ggcggctgcc aggaagatcc tccagagatc
     1201 gcagggaaat atcggagatt atcttgcaat gagagagcag cgctacgtga ggctcgcttc
     1261 ggccaagccg caggtgtatg gaaagtacct gaaggggtgg aagaacagaa taaacagcct
     1321 gcgaaccgtt gtggccgaat tcgagaggga caccatgtac gcccggaacg gcctcacgac
     1381 cgtcaggaaa ggatagggcg ccgccgctgc cggggctacc cgtagccccg cttcaaaccg
     1441 tcgattgtca atgccatacc gctaaaatct gttgactttg gctgtttttt cgggttttaa
     1501 taaggagctt aaactaacgg accaatggag ggagggaaga atggatgcca gcagttatta
     1561 taagagaagg tgaatccttc gagagcgccc tcaagagatt taagaagcag tgtgagaaaa
     1621 ccgggattct ctccgagata aaaaaaagag aacactacga aaaacccagc gtcaagagaa
     1681 agaaaaaaat actcgctgcg aaaaagagag ctctgaaaaa actcaagaga actatggata
     1741 aaggtcttta ctaggagtaa atggactata aatccaaaat tgaagagggt ttgaaagagg
     1801 ctttaaagaa gagggacaac ataagggtgt ccgtgctccg gatgctcctt gcctccataa
     1861 agaacaagga agtcgagaaa ataagacctc tgtcggacga ggagttcttt ggcctcgtca
     1921 agacgtcgat caaacaacac aacgattcca tcgagagctt caggaaaggc gggcgtctcg
     1981 agctcgccga aaaggaagag aaagaactgg agatcctcaa ggagtttcaa ccctcacagc
     2041 tgtcggagga agagatcctc agggagatag aagaggcggt cgcagccgtc ggtgcgacgg
     2101 gccggaagga gatggggaag gtgataaagt tcctcatgga caaattcccg ggaaggatag
     2161 acggcaaagt gttgagtgca atggtgctta aaagactgga gtcgtgatga caatagtcga
     2221 aagcccctat gatcgataag acactatcat atctcgatta cctcaaactc agggatatta
     2281 tactccaatt ttctgcgacg cccttctcca aggacgccct tggtaatctt cggcctcttg
     2341 acgatatcga agagatcgcc aggcgccatg accggataga agccgtcctg gatgtcataa
     2401 agtgggacgg cagggtcccc ttctccgacg tccccgatat aaccggagtg ctggcgagga
     2461 ccgccatcag ggattccgtc ctcgagccct cggaattcct gtccctcaca aatttcctga
     2521 gggcctgcga agacgttggg ggttttctct cgaaggtgtt caaaaagagc gccttcaccg
     2581 acgagctcgt tgccgggatc gacaggctga ccgcactcgc gaggcgtatc gcgcgttccg
     2641 tgaattccga gggttacctc gaagacagcg cgtcctacga gttgtcacgc atacgggccg
     2701 atctcttcat gaacagggag aggatcagga aacagcttga acgcataatg ggccgggagg
     2761 ccatgcgtcc catcatccag gatgactata tatccctgcg gaacaacagg tacgtcattc
     2821 ccctgaagcc gaacttcaac gaggccgtcc agggcatcgt gcacgactat tcccattcgc
     2881 tcaagacctc ctttgtcgag cccgtggagt gcgtggaact caacaacagc atcaacatcc
     2941 tcatcaacga ggaaaaggag gaggaaaaga aggtcctccg cgagcttacc gatttcacgc
     3001 ggggcttcag ggaggccctg gaaaggaacg tacggtgcct cgccgagctc gacttctttc
     3061 acgccatggc gctcttctgc catgaattca ggtgtgtcag gcccgaggtg acgctgtcgg
     3121 gaagccttga ggtgaagaag gccgtcaatc cattcatagc gatgtcccgc aagagcgaag
     3181 cggtgcccat cgacatcgtt atgaaggaga gcgagaaggt gatgatcatc agcggtccca
     3241 acgccggggg caagaccgca gcgctcaaga cgatcggcct gctctcgcta atggcgaaga
     3301 caggcctttt cattcccgcc accgaaagac cggtggttcc cctcttctcg aatgtcttcg
     3361 cgctgatcgg cgacgagcag gacatatcgc tggaactgag cagcttcact gcccacatgt
     3421 atgccattaa ggacctctat ctcaacgccg ggggtgacga actcgttctc attgacgaga
     3481 tagggggcaa cacggagccc caggaagcat ccgcactggc gatgggggtc atcgacgcct
     3541 tcgtggaaaa gggatgccgc gttgtcgtca cgacccatct caatctcatc aaggcctacg
     3601 ggtatatgaa ggattacgcc atgaatgtgg cgacatcctt cgacaccacg aacatgaggc
     3661 cgctttacca gctcatttac gggaccgccg gttactcgaa cgccatcaat gtcgcgagga
     3721 agatcgatgt ccctcaggtc ataatagaga agagctacac ctacctcaac gagcaggaat
     3781 tcatgctcaa cgatctcata acgagccttg aggaggagaa ggcaaagacg gcacgggaac
     3841 gcggggaact ggcgaagatc aaggaagagg cccgcgagag gttgaaggtc atcagggaga
     3901 ggaaggatga ctacctgagg aaatgggagg acaaggctcg cgagaggctc ctcgaggtcg
     3961 aggtcgaggt cgaggagatc aaaaaagagg ttgcaaaaaa ggaaagagcg tctataacaa
     4021 agagtaagga caaaatacgt tcccttaagg aaaggctgag gggacccgct aaggaggtcg
     4081 gggaggacat ccggataggc gattacgttc tcgtgaagac gctgggaagc aaagggtacg
     4141 tcgtcgatat cgacaaagag ggaagtgttt tcgaagttgc cgtaggcaac gtcaggacga
     4201 agctgaaacg gatgttcatc gagaaggcta tcgaaggccc aaaacgggtg tcgcgcgaca
     4261 gatccgagat caacgtggag aaaacggagc agcgggacct gaagatcatc ggcatgcggg
     4321 tggaagaggc attgaagacc gtcgatacct tcctcgatcg ggccgttgtc gagggtactt
     4381 cacaggtcag gatcgtgcat ggtgtgggca cgggacggct catgcaggcc gtgagggacc
     4441 acctcgccga gacgtcttat gtccgcgcct tccatgcgga cgagcgcaat gccggcgtca
     4501 ccatagtgga gttcgcatga aagggtcact cgacgagctt ctgcagagaa tgaacatcat
     4561 cgacgtcata tccca
//
LOCUS       DAOQTR010000113         4562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21110_length_4562_cov_3.276681, whole genome shotgun sequence.
ACCESSION   DAOQTR010000113 DAOQTR010000000
VERSION     DAOQTR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21110_length_4562_cov_3.276681"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..390
                     /locus_tag="PKN85_03880"
     CDS             52..390
                     /locus_tag="PKN85_03880"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HNT43574.1"
                     /translation="MKATFVEGGPLNVLTLARDRVHAGWLLLTHPLYGNIQPSQQHFR
                     SVLVGLPDEQREVDLGSLSILERAIGLYGAEGTHPVRPARVDRYLDDFAALDVYLLRD
                     SMERYGLLKE"
     gene            460..783
                     /locus_tag="PKN85_03885"
     CDS             460..783
                     /locus_tag="PKN85_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT43575.1"
                     /translation="MIELNKENFEEEVLKSDLPVLVDLWGPQCGPCLALMPKVEEISR
                     KYEGKVKFCKLNVAENRKLVISLRVMGVPTFLFYKGGKEDGPRLSGAEAVAEKIEEKV
                     RQLAG"
     gene            814..2100
                     /locus_tag="PKN85_03890"
     CDS             814..2100
                     /locus_tag="PKN85_03890"
                     /inference="COORDINATES: protein motif:HMM:NF020896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HNT43576.1"
                     /translation="MELKLKKVAVRSLLDGDKTSFSDGVLKVNRREIVDMLMKDKRFS
                     SAMVELALPGESTRILPVKDVVEPRCKLEGPGEVFPGFIGDVETVGEGTTLALVGAAV
                     VTCGKIVGFQEGIIDMSGPGAEYTPFSKTLNVVLVVEPVEGLEKHEYESACRLAGLKT
                     AHYLASAAKGATGGEVETYCLPDLAKAMTSFPGLPKVAYLYMLQSQGLLHDTYVYGVD
                     AKRIIPTLIHPNEVMDGAIVSGNCVSACDKNNTYSHQNNPIIHHMYKRHGKDLNFVGC
                     VITNENTTLADKKRSSSYAVKLAKMLGVEGVIISEEGFGNPDTDLIMNCRKAEQSGIK
                     TVLVTDEYAGRDGASQSLADACPEADAVVTAANANEIIVLPRMEKIIGLPDTANVIAG
                     GFQGSLREDGSIEVEIQAITGATSELGFSRIGAYTI"
     gene            2145..2621
                     /gene="grdA"
                     /locus_tag="PKN85_03895"
     CDS             2145..2621
                     /gene="grdA"
                     /locus_tag="PKN85_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083775795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2274..2276,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HNT43577.1"
                     /translation="MGKLAGKKVLLLGERDGVAAPAMEESLKNCGADIVFSVTECFVU
                     TAAGAMDLQNQQRVKDAAEKFGAENLVVILGSSDAEGAEIYAETVTNGDPTFAGPLAG
                     VPLGLAVYHVFEQEIRDEADPSVWADQIGMMEMVLETESLANAVKGVREQYGKYTL"
     gene            2656..3963
                     /gene="grdB"
                     /locus_tag="PKN85_03900"
     CDS             2656..3963
                     /gene="grdB"
                     /locus_tag="PKN85_03900"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3700..3702,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HNT43578.1"
                     /translation="MKYKVVHYINQFFAGIGGEEMAGHKPESRKGFVGPGQAFNAALA
                     PDAEIVGTVICGDTYFADNLEKAEKEVLDLIASFSPDAVVAGPAFNAGRYGTACGAVC
                     EAVRKNLGLPTVSGMYEENPGVDMYRKSTYIARTADSARGMKDAVPAMARLLLKQLKG
                     EPIGKPDDEGYIERGVRVNFFMDKIGAERAVDMFVKKLKGEPFTTEYPMPTFDRVPPN
                     PPVADMKSATIALVTSGGIVPKGNPDHIEASSASKYGEYSLAGVMTINSSTHETAHGG
                     YDPTYANDDPNRVLPVDVMRDLEKEGAFKKFHETFYATVGNGTSVGNARRFGTEIAQK
                     LKDANVDAVIVTSTUGTCTRCGATIVKEIERAGLPVVHVCTIVPISLTVGANRIVPAV
                     AIPHPLGDPTKTPEEEKKIRRAIVEKALKALQTPVEDQTVFND"
     gene            4031..>4562
                     /locus_tag="PKN85_03905"
     CDS             4031..>4562
                     /locus_tag="PKN85_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43579.1"
                     /translation="MARVAIRGYSYCLNHTPELGLHYGVTPYIERITHGETEYIKKLC
                     KSMQSYEEACAYAPNQAYIGGMDLDDLKAQPAPMYGNRIDGASRYGKFGEIMPEKEFL
                     GLMDICDVFDIIWLEESFAGEVREVMARHPLMNEKILTRLEKGHASSEIEKETKEHGA
                     LPLYSQGRVAGCCRKGH"
BASE COUNT         1071 a         1256 c         1400 g          835 t
ORIGIN      
        1 ttccaggaga tcgactgcct cacaaacaac gcccggctct gagacatttc cctgaaagcc
       61 accttcgttg agggtggccc ccttaacgta ctgaccctcg cgagagaccg agtccatgcc
      121 gggtggctgc ttttgaccca tcccctttat ggaaatatcc agccctccca gcaacacttc
      181 aggagcgtcc ttgtcggcct gcccgacgaa caaagggagg tcgaccttgg atcgctttct
      241 atccttgaaa gagccatcgg tctttacggg gcggagggga ctcaccccgt gaggccagcc
      301 agggtggacc gctacctgga tgactttgcc gccctggatg tatacctgtt gcgggacagc
      361 atggagagat acggcttgct gaaagagtag cgcctgtgat ggttcctgaa gcggctgtaa
      421 aaccccgaag ggcggagaaa gctgaaggga ggtgaaaaga tgatcgaact gaacaaggaa
      481 aacttcgaag aagaggtcct gaagagcgac cttcctgtgc tggttgacct ttggggtccg
      541 cagtgcggcc cctgcctggc tttgatgccg aaggtagagg agatatcccg caagtacgag
      601 ggcaaggtca agttctgcaa gctcaacgtc gccgagaatc gcaagctcgt catatccctc
      661 agagtcatgg gggtgccgac cttcctgttc tacaagggag ggaaggagga tggaccgagg
      721 ttgagcggag ccgaggcagt ggctgaaaag atcgaggaga aggttaggca attggcaggg
      781 tagcgatggg tatttgaaag gggggaaaca taattggaac ttaagctcaa gaaagtagcc
      841 gtaagatccc ttttggacgg agacaaaact tctttttctg acggggtcct aaaggtgaac
      901 cgacgagaga tcgtcgacat gctgatgaag gacaagaggt tctccagcgc catggttgag
      961 cttgccctgc cgggagagag cacgaggatc ctgccggtca aggacgtggt agagccgagg
     1021 tgcaagcttg aagggccggg agaggtgttc ccgggcttca tcggcgacgt cgagacggtg
     1081 ggcgaaggca ccacccttgc cctcgtcggc gcagccgtgg tcacctgcgg gaagatcgtg
     1141 ggtttccagg aaggcatcat cgacatgtcg ggcccgggag cggaatacac gcccttttcc
     1201 aagaccctta acgtggtgct cgtcgtcgaa cccgtcgaag gccttgaaaa gcatgaatac
     1261 gagtccgctt gcaggctcgc cggcctgaag acggcacact acctcgcgag cgccgccaag
     1321 ggtgcgaccg ggggagaagt cgaaacctat tgcttgccgg atctcgccaa ggccatgacg
     1381 tcctttccgg gactcccgaa ggtggcctac ctgtacatgc tgcagtccca ggggcttcta
     1441 cacgacactt atgtctacgg tgtcgatgcc aagaggatca tcccgaccct catccacccc
     1501 aacgaggtca tggacggggc gatcgtctcc gggaactgtg tttccgcctg cgacaagaac
     1561 aatacctatt ctcaccagaa caacccgata atccatcaca tgtacaagag gcatggcaag
     1621 gatctcaact ttgtcggctg cgtcatcaca aacgaaaata ccaccctcgc cgacaagaag
     1681 cgcagttcct cctatgccgt gaaactggcc aagatgcttg gtgtcgaagg cgtgatcatc
     1741 agcgaggaag gcttcggcaa ccccgatacg gacctgatca tgaactgccg caaggctgag
     1801 cagagtggga tcaagaccgt cttggtaacc gacgaatacg cgggcaggga cggggccagc
     1861 caatccctgg ccgacgcctg tcctgaagcc gatgccgtcg tgacggcagc caacgccaac
     1921 gagatcatcg tgctgccaag gatggagaag ataattgggc tcccggacac ggccaatgtg
     1981 atcgccgggg gtttccaggg ttccctgagg gaggacggtt ccatcgaggt ggagatccag
     2041 gcgataaccg gagcgaccag cgagctcggc ttcagccgga tcggcgctta cactatctag
     2101 gtttctgctt caagaatctt tgactttaaa aggaggtgcc tgatttgggc aaactcgctg
     2161 gcaagaaggt gctccttctg ggcgagaggg atggagtggc tgcccccgct atggaggaga
     2221 gcctgaagaa ttgcggtgcg gatattgtct tctccgtgac ggagtgcttc gtctgaaccg
     2281 ccgcgggagc catggacctg caaaatcagc agcgcgtcaa ggacgcggct gagaagttcg
     2341 gggcggagaa cctggtagtg atcctgggtt cttccgacgc cgagggagcc gaaatatatg
     2401 ccgaaacggt gaccaacggc gatcccacct tcgcaggtcc tctggcagga gtcccgttgg
     2461 gactcgcggt ataccacgtt tttgaacagg agatccggga cgaggccgac ccatcggtct
     2521 gggccgacca gatcggcatg atggagatgg tgctcgagac cgagagcctc gccaacgccg
     2581 tcaagggagt tcgtgaacag tacgggaagt acaccctcta gagggcagcc ccgaagaaag
     2641 gggggaaatg atcatatgaa gtacaaggta gtccattata tcaaccagtt cttcgcaggc
     2701 atcggcggtg aagagatggc tggccataag ccggaatcca ggaagggttt cgtaggaccc
     2761 ggccaagcct ttaacgcggc cttagcccct gacgcggaga tcgtgggaac ggtgatctgc
     2821 ggggacacct acttcgccga taaccttgaa aaggctgaga aggaggtcct ggacctgatc
     2881 gcgtcctttt ctcccgacgc ggtcgtcgcc gggccggcct tcaacgccgg ccgttacggc
     2941 accgcctgcg gcgccgtctg cgaagcggtc aggaagaacc tcggtctacc gaccgtgtcg
     3001 ggtatgtacg aggagaaccc cggcgttgac atgtaccgta agtcgaccta catcgccaga
     3061 acagctgaca gcgccagggg catgaaggat gcggttcctg ccatggcccg ccttcttctc
     3121 aagcaactca agggcgagcc gataggcaag cccgatgatg aaggctacat cgaaagaggc
     3181 gtaagggtga acttcttcat ggacaagatc ggggccgaga gggccgtgga tatgttcgtg
     3241 aagaagctca agggagagcc cttcacaacg gaatatccca tgccgacctt tgacagggtg
     3301 cctcccaacc cgccggtggc tgacatgaag agcgcgacca tcgccctcgt gacctccggc
     3361 ggcatcgtac cgaagggcaa ccccgaccac atcgaggcat cgagcgcgtc gaagtacgga
     3421 gaatacagcc tggccggcgt catgacaata aactcttcga cccatgagac ggcccatggc
     3481 ggctacgacc ccacctacgc gaacgacgat cccaacaggg tcctgcccgt cgacgtgatg
     3541 cgggaccttg aaaaagaggg tgccttcaag aaatttcacg agactttcta tgcgaccgtc
     3601 ggcaacggca cctccgtggg caacgccagg cgttttggga cggagatcgc ccagaagctc
     3661 aaggacgcca acgtggacgc cgtgatagtg acctccacct gagggacctg cactcgttgc
     3721 ggcgcaacga tcgtcaaaga gatcgaaagg gcggggcttc ccgtcgtcca cgtctgcacc
     3781 atagtgccca tttcactcac cgttggagcg aacaggatcg tgcctgcggt ggccatccct
     3841 caccccctgg gggacccgac gaagaccccc gaggaggaga agaagatcag gcgtgccatc
     3901 gtggagaaag ccctcaaggc tctccagacg cccgtagagg accagacggt ttttaacgat
     3961 taagtaccca gggatggccc ggatccttat ccgggccatc cgtctataga aacggaggga
     4021 ggagcggccc atggcccgtg tcgctatcag gggttattcc tattgcctaa accatacccc
     4081 ggaactgggg cttcactacg gggtcacccc gtacattgag aggatcaccc acggagagac
     4141 ggagtacata aaaaaactgt gtaaaagcat gcagtcctat gaagaagcat gtgcctacgc
     4201 ccccaaccaa gcctatatag gggggatgga cctggatgac ctgaaggcgc agcccgcccc
     4261 gatgtacggt aacaggatcg atggcgcctc gcggtacggc aaattcggcg agatcatgcc
     4321 cgagaaggag ttcctcggct tgatggatat ctgcgatgtc ttcgatatta tctggctcga
     4381 agagtccttc gccggtgaag tccgcgaagt catggccagg cacccgctca tgaacgagaa
     4441 gatcctgacg cggttggaaa aaggtcatgc ctcttcagag atagagaagg aaacgaaaga
     4501 acacggtgcc cttccacttt attcccaagg cagggtggcc ggttgttgca ggaaggggca
     4561 tg
//
LOCUS       DAOQTR010000114         4556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21142_length_4556_cov_3.200178, whole genome shotgun sequence.
ACCESSION   DAOQTR010000114 DAOQTR010000000
VERSION     DAOQTR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21142_length_4556_cov_3.200178"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..946
                     /locus_tag="PKN85_03910"
     CDS             29..946
                     /locus_tag="PKN85_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT43580.1"
                     /translation="MQRSFSICSRVLLTLLAVFLLQGQASAADKLTRIAENVYAYVDT
                     KGASPKNSFGANAGIIIGRDGIVVVDTLISAREAKRFIRDIRAVSKKPIRYVINTHYH
                     LDHVFGNSEFVKLGAVIVAEENDRREMERSAAETLKNIKGYGLTREDMKGTKPAYPTV
                     TYSERMTIDIGGERVELIHARHSHTGGDTLVYLRDKKVLFAGDILFTDYHPFIAEGNI
                     EEWVKELDEIASMDVEKIIPGHGPVSGKKDVQAMKEYIIAFDRMAKELAAKYGDPAKI
                     AAELKSGLPERRESAFLIGANVEMKYLKK"
     gene            1068..1637
                     /locus_tag="PKN85_03915"
     CDS             1068..1637
                     /locus_tag="PKN85_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43581.1"
                     /translation="MCRICDVGLVEDPASVGDEGDVYFRDHHDVDPSFPAGHVFLVEV
                     GGEEEFEAVRSVLIDAGIPFVVQGTTAAPHDAGVSGVFAVHTPARIAVPREYMGQARD
                     VLEARIFRPVSELRVKALETRLFSDIIWSAVWFAAGFGIACLVVPGDWDQGLRFLVYA
                     VAGLVAVPVGNAALRPGRGAKEKHPWLNE"
     gene            1694..1897
                     /locus_tag="PKN85_03920"
     CDS             1694..1897
                     /locus_tag="PKN85_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002856364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNT43582.1"
                     /translation="MPYVNIKVTKEGVTAEQKEKLIRGVTQLLFDVLGKKPQTTYVVI
                     DEVETDNWGVGGETVTVRRKRVS"
     gene            complement(1898..3061)
                     /locus_tag="PKN85_03925"
     CDS             complement(1898..3061)
                     /locus_tag="PKN85_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT43583.1"
                     /translation="MARFEEFRGKALVFLLFLLFVWFVSFSIRIVFSPILPLVEDEFG
                     VHHAKASAIFTFLSAGYGLSVILSGLLSGRVGYKRTIVLSLSLLSILAFLIPAVHTFY
                     LLYFFAFFLGFAHGMYIPSAIPLITEYYTEKNWGKAIAIHDTGASLAIFATPLVVLFL
                     LHFFPWRGIFVVYGCVFVLVLSVFCLSSTEVRIHDPARALFREIIRIRSLWFMTCIWV
                     FGAGSNLGIYSITPLYLTKELGLTIDYANTILAVSRLVSIGVAVACGFIVDRFSLRRL
                     MFFLLAVTSVFTVLLGLSPVRHTGVLLFFQAFFVTGFFPVGLVAIARTFSREMRGLAT
                     GIILSSSFFLGGGVMPWLLGVSGDLYSFRLGITALGVLTLLASTFVFRLRELK"
     gene            3229..3387
                     /locus_tag="PKN85_03930"
     CDS             3229..3387
                     /locus_tag="PKN85_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43584.1"
                     /translation="MAEATYICRRCGITFSAIPGEEEPPECPECEKNETEKLEESSVV
                     IPPEPPKS"
     gene            3504..>4556
                     /locus_tag="PKN85_03935"
     CDS             3504..>4556
                     /locus_tag="PKN85_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43585.1"
                     /translation="MDLKSLLEARRDEIMGEWVRRILTEVSERYSRRPVEEVRETISE
                     ATEAYFAALTEDDFSKMDRFIEKITAMRLDVGFALSEVQGAFELYRTVLVPIFLTELD
                     TSLLPDALQRLNRCLSHTIRRFSDHFQVQHEQSIREHAGNLERVVGERTRELAESEAK
                     YRMLVEEINDGYFIARKGRLVFANRAFCEMHGYSAEEVIGRPYLDFVAPESRGQLTRI
                     RGRRASTGEAPDQYIYLRLHKDGRKLYSENKVKVITYDGATATAGICRDVTERVTLEK
                     QTLRLVELENERKTIALSTLRQLMATLSHYLLNANAIIGGMARRCVRAASDEERHSSM
                     EAIREQTGKTEGVIAAL"
BASE COUNT         1090 a         1190 c         1487 g          789 t
ORIGIN      
        1 agcgccggaa cgatcaggga ggacaagcat gcaaaggtct ttttccatct gctcaagagt
       61 gttattgacg cttctcgcgg tgtttctgct ccagggacag gcatctgcgg ccgacaaatt
      121 aacacgcatc gccgagaacg tgtacgccta tgtggatacg aagggggcgt cgccgaagaa
      181 cagcttcggg gccaacgcgg ggatcatcat aggcagggac ggcattgtcg tcgtggacac
      241 cctcataagc gccagagagg cgaagcgctt cataagggac atccgggccg tgtcgaaaaa
      301 gcccatcagg tatgtcatca atacccatta tcacctggac catgtcttcg gcaattcgga
      361 gttcgtcaag ctcggggccg tgatcgtcgc tgaggagaac gacaggaggg agatggaaag
      421 gtccgccgcc gaaacgctga agaacatcaa aggatacggt ctcacccggg aggacatgaa
      481 gggcacgaag cccgcctacc ccacggtgac ctattcggag aggatgacca tcgatatagg
      541 cggtgagcgg gtggaactca tccacgcacg ccactcccac accgggggag acaccctcgt
      601 ctacctccgc gacaaaaagg tcctctttgc cggcgacatt ctctttacgg attaccaccc
      661 gttcatcgcc gagggaaaca tcgaggaatg ggtgaaagag ctcgatgaga tcgcgtcgat
      721 ggatgtcgag aagatcatcc cggggcatgg ccctgtgtcg ggcaaaaagg acgtccaggc
      781 catgaaggag tacatcatcg ccttcgacag gatggcaaag gagctcgcgg cgaagtacgg
      841 cgatcctgcg aagatcgcgg cggaactgaa gagcggcctg cccgagcgcc gcgaaagcgc
      901 cttcctcatc ggggcgaacg tggagatgaa gtatttgaag aaatagcttg agtcgcttga
      961 gtcgcttgaa tagcttgagg ggcggaggca aacattcaag gcgttccagg aggagatgag
     1021 tcattctttg cccgcgttgt cgtacgagat atggtgaagg gataacagtg tgccggatct
     1081 gtgatgtcgg gcttgtcgag gacccggcat ccgttggtga cgaaggtgat gtctattttc
     1141 gcgatcatca cgatgtggac ccgtcgttcc ccgcgggcca cgtcttcctt gtcgaggttg
     1201 gcggagagga agaattcgag gccgtccggt cggtgctcat cgatgcgggg atccccttcg
     1261 tcgtgcaggg gacaacggcc gcgccacatg atgccggggt ttccggtgtt ttcgcggtac
     1321 atacgcctgc cagaatagcg gtgccgaggg aatacatggg gcaggcgaga gatgtccttg
     1381 aggcgcgtat cttccggccg gtatcggaac tgagggtgaa ggccctggaa acgaggctct
     1441 tctccgatat catatggtcg gcggtgtggt ttgcggctgg gttcggcatt gcctgcctcg
     1501 tcgttcccgg tgactgggat cagggcctgc ggttcctggt gtatgccgtc gcgggcctcg
     1561 tcgcggtacc tgttgggaac gcggcgttga ggccggggcg gggcgcgaaa gagaaacacc
     1621 cttggttgaa cgagtgaccg ggcggcttaa agccccgtct gccggacggt cacatggata
     1681 ggggagggca acgatgccgt acgtgaacat caaggtaacg aaagaagggg tcaccgccga
     1741 gcagaaggag aagctgatca ggggcgtgac gcagctcctc ttcgatgtcc tgggaaagaa
     1801 gccgcagacg acctacgttg tcatcgacga ggtggagacg gacaactggg gagtgggcgg
     1861 tgaaacggtc acggtaagga gaaaacgcgt gagctgatca tttcagttcc ctgaggcgga
     1921 acacgaacgt gctcgcgagc agggtgagca cgcccagggc cgtgatcccc agtctgaagc
     1981 tgtagaggtc ccccgaaacc ccgagaagcc agggcatgac gcctccaccc aggaagaaac
     2041 tggaggaaag gatgatgccc gtcgccaggc cccgcatctc cctcgagaac gttctcgcga
     2101 tggcgacaag ccccaccggg aagaaacccg tcacgaagaa ggcctggaag aagagaagga
     2161 cacccgtgtg cctcacgggt gacaggccga gaagaacggt gaagaccgac gtgacggcca
     2221 ggagaaagaa catcagcctc ctgaggctga accggtcgac gatgaaaccg cacgccaccg
     2281 ccacgccgat ggacaccagc cgcgatacgg caaggatggt gttggcgtag tcgatcgtca
     2341 accccagctc cttagtcagg tacagtggag tgatcgagta gatcccgaga tttgaccccg
     2401 ccccgaagac ccagatgcag gtcatgaacc agagtgagcg tatcctgatg atctccctga
     2461 acagggcccg cgcgggatcg tggatcctga cctcggtgct ggagaggcaa aagacgctaa
     2521 ggacgaggac gaagacgcac ccatagacga cgaatatccc ccgccaggga aaaaagtgga
     2581 ggagaaagag gacgacgagc ggtgtggcga agatggcgag agaagccccc gtgtcatgga
     2641 tggcgatcgc cttgccccag ttcttctcgg tgtagtactc ggtgatgagc gggatggcgg
     2701 aaggtatgta catcccgtgg gcgaagccaa ggaagaaggc gaagaaatag aggagataga
     2761 aagtgtgaac ggcggggatg agaaacgcca gaatgctgag aaggctgaga gaaaggacga
     2821 tggtccgctt gtatcccacc ctgccggaga gcagcccgga aaggatgacc gaaagaccgt
     2881 atcccgcgga aaggaacgtg aagatggcgc ttgccttcgc gtggtgcacg ccgaactcgt
     2941 cctccacgag agggaggatc ggggagaaga cgatgcggat ggaaaaactg acgaaccaga
     3001 cgaaaaggag aaagaggaga aagacgagcg cctttccccg gaattcctcg aaccgtgcca
     3061 ttaatccaat gtaacccgat aacagacaat ttcaccagaa gtttcacgaa aaatgtgttc
     3121 cccgcccgga ttgtttccgt accgggcatg agggcgaggt ctccttgaca acagctggcc
     3181 cggtgcttat cgatgtacag cacgcaaaca acacgaggag gtgctaagat ggctgaagcg
     3241 acatatatat gcagaaggtg cggcatcacc ttttcggcca tacccggcga ggaggagccg
     3301 ccggagtgcc cggaatgtga gaagaacgaa acggagaaac ttgaggaaag ttcggtggtc
     3361 attcctcccg agcccccgaa gtcctgagga gggtcggtaa gatgcgaagc cccgttgttc
     3421 cgttgctcca tgccgcgatt tcgatcgatg tgatcgcacc atgttcttga cggttgcggg
     3481 gttccggtat aggcttttct gtaatggatc tcaagagcct ccttgaagcg cgccgggatg
     3541 agatcatggg ggaatgggtc aggcggatcc tgaccgaggt gagcgagcgc tattcccgac
     3601 gcccggtgga agaggtcagg gagaccatat ccgaggcaac ggaagcctac ttcgcggcac
     3661 tcacggaaga cgatttttcg aagatggaca ggtttatcga gaagatcacc gccatgcgcc
     3721 tcgatgtcgg cttcgccctc tccgaggtcc agggggcttt cgagctctac aggacggtcc
     3781 tcgtgcccat tttcctcacg gagctcgata cgtctctcct gcccgatgcg cttcaaaggt
     3841 tgaaccgttg cctttcccac accatccgga gattcagcga ccattttcag gttcaacacg
     3901 agcagtcgat acgggagcac gccgggaacc tggagcgggt ggtcggggaa aggacacggg
     3961 aattggcgga atcggaggcg aaataccgga tgctcgtcga ggagatcaac gacggatact
     4021 tcatcgcaag gaaaggaagg ctggtattcg ccaacagggc gttctgcgag atgcacggct
     4081 attcggcgga agaggtcatc ggacggccat atctcgattt tgtggccccc gaatcgcggg
     4141 gccagctgac caggatccgg gggagacggg cgtcgacggg ggaagccccg gaccagtata
     4201 tatacctgag gctccataag gacggcagga agctctattc ggagaacaag gtcaaggtga
     4261 tcacctatga cggggcaacg gcgaccgccg ggatctgccg ggacgtcacg gagagggtga
     4321 cgctcgaaaa acagacgctg cgcctcgtcg agctcgagaa cgaaagaaag acgatcgccc
     4381 tgtcgacgct gcgccagctc atggccacgc tgtcgcacta tctcctcaac gccaatgcca
     4441 taatcggcgg catggcgagg cgctgcgtgc gggccgcttc cgacgaggaa aggcactcat
     4501 cgatggaagc gataagggaa cagacgggca agacggaagg ggtcatagcg gccctc
//
LOCUS       DAOQTR010000115         4554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21149_length_4554_cov_3.789287, whole genome shotgun sequence.
ACCESSION   DAOQTR010000115 DAOQTR010000000
VERSION     DAOQTR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21149_length_4554_cov_3.789287"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKN85_03940"
     CDS             <1..363
                     /locus_tag="PKN85_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT43586.1"
                     /translation="PRDKRWKRREILGALGEILLDRDEERLYPTVAEESCSGCGSCKN
                     ACPQKAIDIMIKERDLSIFGPLSSTSVPIAHIDEGACMACGLCVSTCPSDVIKFPVQE
                     EEHKIEECMAHVPSEKTA"
     gene            complement(681..1196)
                     /locus_tag="PKN85_03945"
     CDS             complement(681..1196)
                     /locus_tag="PKN85_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNT43587.1"
                     /translation="MVIFMTSCLKVLSMLMIFTMLGAIGFATGDLTADKLLVEKKARR
                     LTLLSKGQILRTYKIALGGNPEGPKEREGDGKTPEGSYIIDGRNTRSGYHLSLHISYP
                     NEKDRKRAKELGVSPGAQIMIHGMKNGLGWIGPFHTLYNWTQGCIAVTDAEIDEIAPL
                     VPNGTPIEIRP"
     gene            1681..2370
                     /locus_tag="PKN85_03950"
     CDS             1681..2370
                     /locus_tag="PKN85_03950"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT43588.1"
                     /translation="MMMTTKVSRQRLSNQVYDILKEMIADYRFSPGARINVEQIAKEV
                     GASRTPVWEAVHRLIQEGLLENIPNRGVFMVALRPEKAIELYEVRNALEGLAAGLAAV
                     NIDAKTLKKMKRSLEEQMKVVEAGDLVAYSQLDYEFHALVYEASRNKTLKEMLEEVKS
                     KMRPIGMHITPVLADLYQDHVDLLDSLERHDAHGAEVAFTRHNSRMIELIEKSVGSDT
                     WKGAHENTDKK"
     gene            2417..3667
                     /locus_tag="PKN85_03955"
     CDS             2417..3667
                     /locus_tag="PKN85_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT43589.1"
                     /translation="MKIKVFAVVVLTVLFAAGSLCVIPDASAQDKVRVGLIFGLTGAA
                     SPIGPLQLDGAKLAIKEINAAGGVKLGGKKVPIEFYVKDDESKPDVAIRRFRELQDEN
                     KVNVVVGQTFAPISAALNKEAKKTSIAYFPVNVVAVGMFEKKEMAETTFAIHGSAYSI
                     GYAGASYIVDKLGLKNIIFFGPAYAFGRDQWQGAKDAFEARKVKVEYLESPVGTSDYT
                     SYLLKIKEKKPQIVMLAHWGVDAINVLKQTYEVGIKKNSKVWFNWMTNAFGSGVPPEA
                     LEGVYSLMGWYYDMKGFQDAAIVKASDAFAKKFQAEYKYPPDPYSAMAYEGVREAIRG
                     IELANSLEGKAISKAIMANPTFDSMKGKGTWRADHQPLFKYGAYVTIGKGSKERKNAK
                     WDLVKIIGAYTGEDYLPNLKALGY"
     gene            3738..4517
                     /locus_tag="PKN85_03960"
     CDS             3738..4517
                     /locus_tag="PKN85_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT43590.1"
                     /translation="MSANPIIETKKATIRFNGLTAVDTVDFRMEAGEAVGVIGPNGAG
                     KTTFFNLLTGLYTPTEGAILFSGKDITKVPPHRRVALGIIRTFQLVSVFDSLPVIDNL
                     ALSTIRFGENYGGKGKFFFANVHSQRIMKACMEKLEVLGISDKADLMTSELSYGDKRK
                     LEIAVALSLNPKILLLDEPFAGRSDSEIGEVSVIINRVKKDLSFVIIEHKISRIVDMV
                     GRLCVMHEGRLIADGPPNQVLCDATVREVYWGKEEGLTCSV"
BASE COUNT         1043 a         1295 c         1343 g          873 t
ORIGIN      
        1 ccccgcgaca agcgctggaa gcgccgggaa atactgggtg ccctcggcga gatcctcctc
       61 gaccgtgacg aggaacgcct gtatcccacg gtggccgaag agagctgcag cggttgcggc
      121 tcctgcaaga acgcctgccc gcagaaggcg atcgacatca tgatcaaaga gagggacctg
      181 tccatcttcg gccccttgag ctcaacgagt gttcccatag cccacataga cgaaggcgcc
      241 tgcatggcct gcggcctgtg cgtgtcgacg tgcccgtcgg acgtcatcaa gttcccggtt
      301 caagaggaag agcacaagat cgaggaatgc atggcgcacg taccgagcga aaagacggct
      361 tgaaccgctt aaaccgcttg aacgttaaag gataaaaaag aatagttctg cccgctccgg
      421 tatcctgttg cgaatgcgat cgctccacca ttaaggtacg tggtcatgtc tttcgcttct
      481 cacattcaac agtctgctga caaggtgatt tctgaaggcg gttcgtgctc tcttgtcatt
      541 ccggcgaagg ccttaaatcc aggaaaaacg aggggttaga gaaacctctg gatttaaggc
      601 cttcgccgga atgacgtaga aaatgggttt atcaacagcc tgtcaagcgg tttaagcggc
      661 tcaagcggtt caagccgtct ttatggccgt atctcgatgg gcgtgccgtt ggggacgagg
      721 ggggcgatct cgtctatctc cgcgtcggtg acggcgatgc agccctgggt ccagttgtag
      781 agggtgtgga aggggccaat ccatccgaga ccgttcttca tgccgtggat catgatctgt
      841 gcgccggggg aaacgccaag ctccttcgcg cgcttcctgt ccttctcgtt tgggtaggag
      901 atgtggaggg acaggtggta cccgctgcgg gtgttccgcc cgtcgatgat gtagctccct
      961 tcaggcgtct tcccgtcgcc ctccctttcc ttcggcccct cggggttccc gccgagggct
     1021 atcttatacg tcctcaggat ttggcctttc gataacagcg tcaggcgcct cgcctttttc
     1081 tcgaccagga gcttgtcggc cgtgaggtcc ccggtagcga acccgatggc ccccagcatg
     1141 gtgaagatca tcagcatgga caggactttg aggcagctcg tcatgaagat gaccatacca
     1201 tttcctgggg acctttacca gcgaccgggt ggggaaagac tttatctcac gcccgcttcg
     1261 gtagatagcg cagagaaagg caaatgagag tgtgcggaaa aagattgcgg cagatcgcgg
     1321 agccttgtac gatctgaccg ccttcgcatc ccgtctgcga cgggagggaa aacaggaccg
     1381 cggggatgga attataggac ctctcggaca tataggactt ataagaccta tggcagcagc
     1441 agaaaacggg aaaccccttt cccaggataa gaaaacgata ttctcttctc atcctctgcg
     1501 ccctcgagcg aagcgggcgt gagataacgc ctcggccttt gacccccata ttcgttcctg
     1561 tcaaaatagc ctcttagtct tttgtttcag taccttgtca tactagcaat ttcatgaaat
     1621 atgaaaaata tttcgtgaaa gagcttgaca ggattcccat gcgggggata gaattaccct
     1681 atgatgatga caacgaaggt ttccaggcaa cggctcagca accaggtcta tgacattttg
     1741 aaggagatga tcgcggacta ccgattcagt cccggagcca ggatcaacgt ggagcagatt
     1801 gccaaggagg tgggcgcctc caggacccct gtctgggagg ccgtgcaccg gctgatccag
     1861 gaagggctcc tcgagaacat ccccaacagg ggtgtcttca tggtcgccct gaggcccgag
     1921 aaggccatcg agctctacga ggtcaggaac gcgttggagg gcctcgccgc cggcctcgcc
     1981 gccgtgaata tcgacgcgaa gacgctgaaa aagatgaaga ggtccctcga ggaacagatg
     2041 aaggtcgtag aggcgggaga ccttgtagcc tactcccagc tcgactacga attccacgcc
     2101 ctcgtctacg aggcaagccg gaacaagacg cttaaggaga tgcttgagga ggtgaagagc
     2161 aagatgcggc ccatcggcat gcacatcacc cccgtcctgg cggacctcta ccaggaccac
     2221 gtggatctcc tcgactccct ggagaggcac gacgcgcacg gcgccgaggt ggccttcacc
     2281 aggcacaact cccgcatgat cgagctcatc gagaaaagcg tcggctccga cacctggaag
     2341 ggagcccacg agaatacgga caagaaatga tagtcgaagg gatcttcaca tcatgaaagg
     2401 aggaatcgca atgtccatga agatcaaggt ttttgcagtc gttgttctga cggtactttt
     2461 cgcggcgggg tcgctgtgcg tgatacccga cgcctccgcg caggacaagg taagggtcgg
     2521 tctcatattc ggactcaccg gagcggcatc acccatcggc cccctgcagc ttgacggggc
     2581 caagctggcg ataaaggaga tcaacgcggc gggcggcgtg aaactgggcg gcaagaaggt
     2641 ccccatcgaa ttctacgtca aggatgacga gtcgaagccc gacgtcgcca tcaggcgctt
     2701 cagggaactc caggatgaga acaaggtgaa cgtcgtcgtc ggccagacct tcgcgcccat
     2761 ctccgcggcc ctcaacaagg aggccaagaa gacctccatc gcctatttcc ccgtgaacgt
     2821 ggtggctgtc ggcatgttcg agaagaagga gatggcggag acgaccttcg cgatccacgg
     2881 cagcgcctat tcgatcggct acgcgggagc ctcctacatc gtcgacaagc tgggtctcaa
     2941 gaacatcatc ttcttcgggc ctgcctacgc ctttggccgc gaccagtggc agggtgcgaa
     3001 ggacgcattc gaggcccgca aggtcaaggt ggagtacctg gaatcacctg tgggaacgag
     3061 cgactacacg tcatacctcc tgaagatcaa ggaaaagaaa ccgcagatcg tcatgctcgc
     3121 ccactggggt gtcgacgcga tcaacgtcct caagcagaca tacgaggtcg gcatcaagaa
     3181 gaactccaag gtgtggttca attggatgac gaacgccttc ggaagcggcg taccgccgga
     3241 agcgctggag ggcgtctact ccctcatggg ctggtactat gacatgaagg gtttccagga
     3301 cgcggcgatc gtcaaggcgt ccgacgcctt cgcgaagaaa ttccaggcgg aatacaagta
     3361 tccccccgat ccctattcgg caatggccta cgagggcgta agggaagcca tccgcggcat
     3421 cgagcttgcc aattcactcg agggcaaagc gatatcgaag gccatcatgg ccaaccccac
     3481 cttcgattcc atgaagggaa agggaacgtg gcgggccgac catcagccgc tgttcaagta
     3541 cggtgcctat gtcaccatcg gcaaggggtc caaggagcgg aagaatgcca agtgggacct
     3601 cgtgaagatc atcggggcct atacgggcga ggattacctt ccgaacctga aggctctcgg
     3661 atactagcat aggacacgac ggacggggcg gatacgcgtc cgccccgtcc ccgagaacag
     3721 cacgacggga gtttcagatg tcagcgaacc cgataatcga gacgaaaaag gcgactatac
     3781 gcttcaacgg actcacggcg gtggatactg tcgatttccg gatggaagcc ggagaggcgg
     3841 tgggcgtcat cggccccaac ggcgcgggga aaacgacgtt cttcaacctc ctcacgggcc
     3901 tgtatacccc gacggagggc gccatcctct tctcgggcaa ggatataaca aaagtccccc
     3961 ctcacaggag ggtggccctg gggatcatac ggacctttca gctcgtctcc gtcttcgatt
     4021 ccctccccgt tatcgacaat ttggctcttt ccaccatccg tttcggcgag aactacgggg
     4081 ggaagggcaa gttcttcttc gccaacgtcc acagtcagcg catcatgaag gcctgcatgg
     4141 agaagctcga ggtcctgggc atctccgaca aggcggacct catgacatcg gagctttcct
     4201 acggggacaa gcgcaagctc gagatcgccg tcgcgctgtc cctcaacccg aagatactcc
     4261 ttctcgacga gcccttcgcg ggccgctccg acagcgagat cggcgaggtc tccgtcatca
     4321 tcaaccgcgt caagaaggac ctcagtttcg tcatcatcga acacaagata tcgaggatcg
     4381 tcgacatggt gggcaggctc tgcgttatgc acgaaggccg gctcatcgcc gacggcccgc
     4441 cgaaccaggt gctctgcgat gccaccgtcc gggaggtcta ctggggcaaa gaggagggac
     4501 tgacatgctc agtgtgaaga atatcgacgt cgcctacgga catgcccggg tcct
//
LOCUS       DAOQTR010000116         4531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21306_length_4531_cov_3.140527, whole genome shotgun sequence.
ACCESSION   DAOQTR010000116 DAOQTR010000000
VERSION     DAOQTR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21306_length_4531_cov_3.140527"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKN85_03965"
     CDS             <1..794
                     /locus_tag="PKN85_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43591.1"
                     /translation="DVSGPIVKRYSVDDIHNETAVSHERIASLVSSLRERGILRETEY
                     QETSYFALANRVLREPLWRILKLHQFEDRKMLRNILGNFIVNEMFLDEETLDLAERWQ
                     DRLVMSKEEMGITLASLIARGRDWKSLFETANRFERGIDIQPILKLLYSKSEEKRKNA
                     ISLLVEINDKDMINPLLLHLKEEDVLEIKDLVIQGIGHTGKKRAIIAIMNTLKEIGDR
                     DLKLRALTFFESLFGDNSRNILSDIKEIEEDPVITRKIDELLRMA"
     gene            complement(1080..2351)
                     /gene="serS"
                     /locus_tag="PKN85_03970"
     CDS             complement(1080..2351)
                     /gene="serS"
                     /locus_tag="PKN85_03970"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNT43592.1"
                     /translation="MIDPRMIRENIEAVNAALANRQASFDAGRLVELDGERRRLIQET
                     EKLKNEKNTLSNEIAKLRKSGADATDKIEYQRSLGKRIEETGQSLKAVEEEWEQLLLV
                     IPNMPDPSVPVGSCDDDNKVVKTWGEKPSFDFEPKAHWDLGEGLDILDFKRAAKITGS
                     RFVLYKASGALIERALINFMLDLHVGEHGYTEVLPPFMVNRTSMTGTGQLPKFEEELF
                     HLEGVDYFLIPTAEVPVTNIHADEILKEEELPIKYVSYTPCFRKEAGAHGKDTRGLTR
                     LHQFNKVELVKFAHPDNSFDELEGLLLDAEDVLKKLNIHYRVSALCTGDLGFSSAKTY
                     DIEVWLPGQNIYREISSCSCFTDFQARRAKIRFRRQGGKTELVHTLNGSGLAIGRTVM
                     AVMENYQNADGTITIPEALRPYMHGKDKIMA"
     gene            complement(2352..2921)
                     /locus_tag="PKN85_03975"
     CDS             complement(2352..2921)
                     /locus_tag="PKN85_03975"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNT43593.1"
                     /translation="MRGKKDIYIGGKPPAHSKLIRTPYIIVLIVIAVAAAGFFAYRYA
                     RLARPLKTVVVEEVSDLKVYYPSRPAKLSLKSTPVKTALTDREKADAIISALKTGKVL
                     PDTLVLTDFAADMEGTLILNFTSDLALLKLEPLTEIQTVYAIVNSFLANFTKAKNVQL
                     LAAGQAIFTIGGTVYTYKPMEFNSQMLED"
     gene            complement(2918..3484)
                     /locus_tag="PKN85_03980"
     CDS             complement(2918..3484)
                     /locus_tag="PKN85_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HNT43594.1"
                     /translation="MKPAIIIVDMLEGNYTKPITGIKEEEKIVPVVRDFLKIVRGLDI
                     PVIFACDSFLENDFIFEGRMKPHAIRGTEGTKPLSELEPQPSDIVLDKRRFSAFFKTD
                     LDQTLRTLGVDTVAVGGINTHFCVLATALDAICNDFATIILEDMSAAYKREIHENFLD
                     AYRKNVLYPIFRVMRSSDLIEEFEREVQ"
     gene            complement(3481..4263)
                     /locus_tag="PKN85_03985"
     CDS             complement(3481..4263)
                     /locus_tag="PKN85_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT43595.1"
                     /translation="MFVDSHCHLEMEDFDRDLDVVVRNCVDEGLEYVLTVGTEESYFG
                     KVRGLIERYPFIYGAIGIHPHNAKDFSERTEDVIREAARHPRIVGYGEVGLDFFKNHS
                     PREVQEKIFVRQLGLAQELGLPIIVHSRESKERTLDILKKEYPSGYGVIHCYSYDVAA
                     ARQFLDMGFHISIPGTVTYKTAGSLRDVVRFVPVDRLLAETDAPFLSPVPHRGKRNIP
                     SHVKITIEAMAKERGVKTGELAPLLRDNFVRLFLTDPRKDRS"
BASE COUNT          931 a         1232 c         1329 g         1039 t
ORIGIN      
        1 tcgatgtttc gggccccatc gtgaaacggt acagcgttga cgacatccac aacgagaccg
       61 ccgtctccca cgaacggata gcctcgctgg tctcttcgtt gagggagcgc gggatcctgc
      121 gcgagacgga gtaccaggaa acatcctact tcgccctcgc gaacagggta ctcagggagc
      181 ctctctggcg catcctgaaa ctccatcagt tcgaggacag aaagatgctc cgcaatatcc
      241 tgggcaattt catcgtcaac gaaatgttcc tcgacgaaga gaccctcgat ctggccgaga
      301 ggtggcagga ccggctcgtg atgtcgaagg aggaaatggg catcaccctg gcaagcctta
      361 tcgctcgcgg aagagactgg aagtctctct tcgagacggc caacaggttc gagcggggca
      421 tcgatatcca gcccatcctg aagcttctct acagcaagag cgaggagaag agaaagaatg
      481 cgatcagcct cctggtggag atcaacgaca aggacatgat caaccctttg ctcctgcacc
      541 tcaaggaaga ggatgttctg gagatcaagg acctcgtcat ccagggcatc ggccacacgg
      601 ggaagaagag ggccatcata gctatcatga acaccttgaa agagatcggg gacagggacc
      661 tcaagctccg cgccctaacg ttctttgaat ccctgttcgg cgacaactcc cgcaacatcc
      721 tcagcgacat caaagagatc gaagaagacc cggtcatcac caggaagatt gacgaactcc
      781 taagaatggc ttgaatagct tgagtcgctt gaatcgctta agaggctgtt gagaaagtcc
      841 tctttgtcgt catcccggac ctgatccggg atccagaggt ttttccaacc cacggttttc
      901 ccgggatatc ccttactata gtgaaaacac gggcctaaag ccagaatgac gcaagaacat
      961 aaaagcgggt tcagaaacaa ctttatcaac agcctgttaa gggttaaaaa atgacccctg
     1021 atgcccggga gggctgtcag gggtttggac tttcaagcga ctcaagctat tcaagcgact
     1081 caagccatta ttttgtcttt tccgtgcatg tacggcctta aggcctcggg gatggttatc
     1141 gtgccgtcgg cgttctggta gttttccatg acggccatga ccgtcctgcc tatggcgagg
     1201 ccggagccgt tcagggtgtg gacgagttct gtctttcctc cctgcctgcg gaagcggatc
     1261 ttcgccctgc gtgcctggaa gtcggtgaag cagctgcatg acgatatctc gcgatagatg
     1321 ttctgcccgg ggagccagac ctctatgtcg taggtcttgg cggaggagaa cccgaggtct
     1381 cccgtgcaga gggcgctgac gcggtaatgg atgttgagtt tcttgaggac gtcctcagcg
     1441 tcgagcagga ggccttccaa ctcgtcgaag gagttgtcgg gatgggcgaa cttgacgagc
     1501 tccaccttgt tgaactggtg caggcgggtg agcccccggg tatccttccc gtgtgctccg
     1561 gcttccttgc ggaagcaggg ggtgtatgag acatacttga tcgggagttc ctcttccttg
     1621 aggatctcat cggcatggat gttcgtcacg ggcacctcgg cagtgggtat gaggaaatag
     1681 tccaccccct ccaggtgaaa gagctcttcc tcgaatttcg gcagctggcc cgtgcccgtc
     1741 atggaggtgc ggttgaccat gaagggcggc agcacttcgg tgtacccgtg ctccccgacg
     1801 tggaggtcga gcatgaaatt gatgagcgcc ctctcgatga gcgcccctga cgctttgtag
     1861 aggacgaagc gcgatccggt gatcttcgcg gcgcgtttga agtcgaggat gtcgagaccc
     1921 tctccgaggt cccagtgggc ctttggttcg aagtcgaagg aaggtttctc gccccaggtc
     1981 ttgacgacct tgttgtcatc gtcgcatgat cccacgggca ctgacgggtc cggcatgttg
     2041 gggatgacga ggaggagttg ttcccattcc tcttcaaccg ccttgaggct ctgtcccgtt
     2101 tcttcgatcc tcttgcccag cgagcgctgg tactctatct tgtccgtggc gtcggcgcct
     2161 gacttcctca gcttcgctat ctcgttggag agggtgttct tttcgttctt cagcttctcc
     2221 gtctcctgga taagcctgcg acgctccccg tccagttcga cgagcctgcc ggcatcgaag
     2281 gaagcctgtc ggttcgccag ggcggcattc acggcttcga tgttctcgcg gatcattctt
     2341 gggtcgatca tttaatcctc cagcatctga gagttgaact ccatcggttt gtaggtgtag
     2401 acggtcccgc caatggtgaa gatggcctgg cccgcggcta gaagctgaac gttcttcgcc
     2461 ttcgtgaagt tcgcaagaaa ggaattgacg atggcataga ccgtctggat ctccgtcagg
     2521 ggttcgagtt tcagaagggc cagatcggat gtgaaattga gtatcagcgt tccctccatg
     2581 tcggctgcga aatcggtgag gacaagggta tcgggaagga ccttgccggt cttgagggcc
     2641 gatatgatgg catcggcctt ttcgcggtcc gtgagcgcgg tcttgacggg agtgctcttc
     2701 aggctcagtt ttgccggccg ggaagggtaa tagaccttga gatcgctcac ctcttcgacg
     2761 actaccgtct tcaggggcct ggccagtctg gcgtagcggt aggcgaagaa gcccgccgcc
     2821 gcgacggcta tcacgatcag cactatgata tagggggtac ggatgagctt gctgtgagcg
     2881 ggaggcttcc ctccgatgta tatgtccttc tttcctctca ttgcacttct ctttcgaact
     2941 cctcgatgag gtcgctcgac ctcatgacac ggaaaatggg atacagaacg ttcttgcggt
     3001 aggcgtcgag gaaattctca tggatctctc tcttgtaagc ggcgctcatg tcctcgagga
     3061 tgatggtcgc gaagtcgttg catatggcat cgagggcggt ggcgagcaca cagaaatggg
     3121 tgttgatccc tcccaccgcc acggtgtcga cgccaagggt ccggagcgtc tggtcgaggt
     3181 ctgtcttaaa gaaggcgctg aatcgccgtt tgtcgaggac gatgtccgac ggctggggtt
     3241 ccagctcgga aagcggtttc gtcccttccg ttccccggat agcgtggggt ttcatgcggc
     3301 cttcgaagat gaaatcattt tccaggaagc tgtcgcaggc aaagatgacg gggatatcga
     3361 ggccccggac gatcttgagg aagtcccgga cgacggggac gatcttctct tcttccttga
     3421 tgcccgtgat gggtttggtg tagttgcctt ccagcatgtc aacgatgatt atggcgggtt
     3481 tcatgatctg tcctttcgtg gatccgtgag aaagagtctg acgaaattat ctcgcaggag
     3541 tggcgccagt tcaccggtct taacgccgcg ctccttcgcc atggcctcga tggttatctt
     3601 tacatgggag gggatgttcc gttttccccg gtgcggtacg ggtgacagga aaggtgcgtc
     3661 ggtctcggca aggagccggt cgacgggaac gaaacgcacg acgtccctca agcttccggc
     3721 ggtcttgtac gtgaccgttc cggggatgga gatgtggaac cccatgtcca gaaactgacg
     3781 ggcagccgcc acgtcgtagg aatagcagtg gataaccccg tatcccgaag gatactcctt
     3841 tttgaggatg tcgagggtcc gctccttcga ctcgcgcgag tggacgatga tgggcagtcc
     3901 gagttcctgt gccagaccga gctgcctgac gaagatcttc tcctggacct cgcggggcga
     3961 gtggttcttg aagaaatcga ggccgacctc cccgtaaccc actatcctgg gatgcctcgc
     4021 cgcctcgcgg atgacgtcct cggtcctttc gctgaagtcc ttcgcgttat gagggtggat
     4081 gccgatggcc ccgtatatga aaggatacct ctcgatgagt cctcgcacct ttccgaaata
     4141 agattcctcc gtcccgacgg tgaggacata ttcaagtccc tcgtcgacac agttcctgac
     4201 gacaacatcc aggtccctgt caaaatcctc catctcaaga tggcagtggg aatcaacgaa
     4261 catggatttc ttataacata attggacgcg gggcttcaat caaagagggg tatcgggacg
     4321 aacagcggac ccggcttacc cgtactggtt cacaggctga accaatctct acctggtcgg
     4381 ccggtacgta tcgcttgctc cccccgcacc ctcaccaacg atatgaatcc ggatctcctg
     4441 tccgtagccg ggccgcaaca tgacaacatg gcaagatggc acgtggtatg gacatactta
     4501 ccacctctgt gctcctgagg caatacccct g
//
LOCUS       DAOQTR010000117         4518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21377_length_4518_cov_3.729554, whole genome shotgun sequence.
ACCESSION   DAOQTR010000117 DAOQTR010000000
VERSION     DAOQTR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21377_length_4518_cov_3.729554"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PKN85_03990"
     CDS             complement(<1..1197)
                     /locus_tag="PKN85_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNT43596.1"
                     /translation="MIARIPTATVYGIDGIKIDVEVDIAYGLPAFNIVGLPEASVKES
                     KERVRSAIKNAGFEFPGDRITINLAPADVRKEGASFDLPIAVGILAAMGALKREVVEG
                     HLIAGELSLDGSVKPVRGILPVALLTRQEGIGTIVVPVGNGREASVVRDITVLGADHL
                     LEIFHYFKGEGDLVAFTGDGCGIDSALPCDDTDFSDIRDQGQAKRALEVAAAGAHNII
                     MVGPPGSGKTMLSRCLPTIMPALGYAEAVETTKIHSIAGLLGPDRALIAKRPFRSPHH
                     SISDAGLIGGGHVPRPGEVSLAHNGVLFLDEFPEFRRHVLDALRQPLEDGAVTISRVN
                     HAITFPARFMLVAAMNPCPCGHFGDPKRSCTCTATQIYRYRSRISGPLLDRIDIHIEV
                     PPVTIAE"
     gene            complement(1327..2442)
                     /locus_tag="PKN85_03995"
     CDS             complement(1327..2442)
                     /locus_tag="PKN85_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNT43597.1"
                     /translation="MNRKAKAERKGTFRKRRASDRTYFVIMAGGKGERFWPLSTELVP
                     KPFVSITGKKTLIELTVERARRMVPLERIFVVLGREHLPAARRCLPRLPHGNFIIEPV
                     GRDTAPCVGLAAMILHGRDPDAIMVILPSDHYVPDPTAFSRCIRETVKTAGHGDHLVT
                     IGVPPGRPETGYGYIKTGRKVSAPRDADCFEVARYVEKPNIARARRYIREGGYFWNAG
                     IFVWRAEVLLQGLKYHMPDLHAGLLRFERALGTGDKDAADAIFAGLPRVSIDYGLMEK
                     AKNVLMMPAPFTWDDVGTWTSLLRVLPRDDTGNIVRGKTFSIDTTNCVIITDATPVAT
                     LGVSDLIIIASKNGILICDSSKAQEVRQIAKALGKKQ"
     gene            complement(2458..3192)
                     /locus_tag="PKN85_04000"
     CDS             complement(2458..3192)
                     /locus_tag="PKN85_04000"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNT43598.1"
                     /translation="MEDYSAIADIIRERKYLVAFTGAGISVDAGIPAFRGGQGLWEKY
                     DPMEYAEISSFVRRPEKVWVMLREMAEVIFASAPSEAHRALGRLEKMGYLKAVITQNV
                     DGLHQEAGNSNVIEYHGNHRWLVCMNCHTRLPFTPDATTVNPYPQCEKCNAALKPDVV
                     FFGEGIPMMEMARANEEADRCKVMFIIGTSGVVYPAADIPYRAKSKGAVIVEINVERT
                     PFTSSITDHFLKGTASEILPQILKHL"
     gene            complement(3206..4183)
                     /locus_tag="PKN85_04005"
     CDS             complement(3206..4183)
                     /locus_tag="PKN85_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNT43599.1"
                     /translation="MFNSITDIPGIKVGHATDLTGRTGCTAILCEEGAVAGIDVRGSA
                     SGTRQVDALNVGHIVERVHAILLCGGSSFGLDAASGVSRYLEEKGVGFDVGITRIPIV
                     PTAVIFDLLFGDHRIRPTAQMGYEACLNAGEEVEEGSVGAGTGAVVGKLFEMSRAMKG
                     GLGTCSVAMPDGLKVGALVVVNAFGDIIDNVTGRIIAGLRKSQDSLEFANTTNCLKQG
                     IVKKQFGIVNTTLGVVATNAKFTKRDITKVAQMAQGGLIKTINPVHTTFDGDLVFALS
                     MGDIEADINKVGVLAEFVLSEAIKRGIKKADGFGIIPAFRDINKGWKAG"
     gene            complement(4214..>4518)
                     /locus_tag="PKN85_04010"
     CDS             complement(4214..>4518)
                     /locus_tag="PKN85_04010"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNT43600.1"
                     /translation="GMAAHLPGVIASHTTLPVIGVPASGGILKGKDALYSIVQMPKGI
                     PVASVGIDAGTNAAILACEILSIKYDSVAKALIELKKDLKRENEQKSLNIRNKLGN"
BASE COUNT          826 a         1325 c         1419 g          948 t
ORIGIN      
        1 ctcggctatg gttacgggag gcacctcgat gtggatgtcg atccggtcca gcagcgggcc
       61 cgatatgcgg gagcggtacc tgtagatctg ggtggccgtg cacgtgcagg agcgtttcgg
      121 gtcgccgaag tggccgcagg ggcaggggtt catggcggcc acgagcatga atctggcagg
      181 aaaggtgatc gcgtggttga cccgggagat cgtgacggcg ccgtcttcga ggggctggcg
      241 gagggcgtcg aggacatggc ggcggaattc cgggaactcg tcgaggaaga ggacgccgtt
      301 gtgggcaagg ctgacctcgc ccggccgtgg aacgtgcccg ccaccgatga ggccggcgtc
      361 ggatatgcta tgatggggag agcggaaggg ccgcttcgcg atgagggccc tgtccgggcc
      421 gagaaggccg gctatggagt ggatcttcgt ggtctccacg gcctcggcgt agccaagagc
      481 gggcatgatg gtgggcaggc accgggaaag cattgtcttg ccggagcctg gggggccgac
      541 catgatgatg ttgtgcgccc cggccgcggc gacctccagg gccctcttgg cctgaccctg
      601 gtccctgata tcggaaaaat cagtatcatc gcagggtaaa gcgctgtcga tgccgcaccc
      661 gtcccccgtg aaagcgacga gatcgccctc tcccttgaaa tagtggaaga tctccaggag
      721 atggtcggct ccgaggacgg tgatgtcacg cacgacggag gcctccctgc cgtttcccac
      781 ggggaccacg atcgtgccta taccttcctg ccgcgtgaga agggcgacgg ggaggatgcc
      841 ccgaacaggc ttcacggaac cgtcgaggga aagctcccct gctatgagat gcccctccac
      901 gacctctctc ttcaacgcgc ccatggcggc gaggatgccc acggcgatgg gcaggtcaaa
      961 ggacgccccc tccttgcgga cgtcggccgg ggcaaggttg atggtgatgc ggtcgccggg
     1021 gaactcgaag ccggcattct ttatggcgga cctcacccgc tccttgctct ccttcacaga
     1081 ggcctcggga agcccgacga tgttgaacgc cggcagcccg taagcgatgt ccacctccac
     1141 atcgatcttt atgccgtcga tcccgtaaac agtagcagtt ggaatgcgtg cgatcaaagc
     1201 atacctcctt ccgtgatatg cggacgaaag cgcccgctac cgtgtattac ttctcgtgaa
     1261 gaacaaagtg agagaaaaag tgcggatcgg cggcagcgtg gcaggaacgg gcgagagata
     1321 ctgtctttat tgtttcttcc ccagcgcctt tgctatctgg cggacttcct gcgccttgga
     1381 ggagtcacag atgaggatgc cgttcttcga ggcgatgatg atgaggtcgg agacgccgag
     1441 agtggcgacg ggggtggcgt ccgtgatgat gacgcaattg gtggtgtcga tggagaaggt
     1501 ttttccgcgg acgatgttgc cggtgtcatc cctgggaagg acgcgcagaa gggaggtcca
     1561 ggtgccgacg tcatcccacg tgaagggtgc tggcatcatg aggacgttct ttgccttctc
     1621 catgaggccg tagtcaatgg atacccgcgg caggccggcg aatatggcgt cggcggcgtc
     1681 cttgtcgccc gtcccaaggg ccctttcgaa cctgaggagg ccggcatgga ggtcgggcat
     1741 gtggtatttg aggccctgga gaagaacctc ggcgcgccag acaaagatgc cggcgttcca
     1801 aaagtaaccg ccttcccgga tgtatcggcg ggccctcgcg atgttgggtt tctctacata
     1861 tctcgcgacc tcgaaacaat cggcgtcgcg gggcgccgag accttccgtc ccgtcttgat
     1921 ataaccgtat cccgtctccg gccggccggg agggacgccg atggtgacca ggtggtcgcc
     1981 gtggcctgcg gtctttacgg tttccctgat gcaccgcgag aaggcggttg gatcggggac
     2041 gtagtgatcg gatggcagaa tgaccatgat tgcatcggga tcgcgcccgt ggagtatcat
     2101 ggccgccagt ccaacgcagg gtgccgtgtc gcggccgacg ggttcgatga tgaaattgcc
     2161 gtgaggcaaa cgggggagac accttcttgc cgcgggaaga tgctccctgc cgagaacgac
     2221 gaaaatgcgt tcgagaggaa ccatcctgcg ggccctctcg acggtgagct ctatgagcgt
     2281 ctttttcccg gtaatggaaa cgaagggttt gggtacgagt tcggtgctga gcggccagaa
     2341 acgctctccc ttgcctccgg ccattatcac gaaatacgtc ctgtcagagg cccttctctt
     2401 ccggaaagtt ccctttctct ccgccttcgc tttcctgttc atagcgcccc aaccttttca
     2461 gagatgtttc aggatttgcg gaaggatctc cgaggccgtg cccttgagga aatggtctgt
     2521 gatggatgag gtgaaaggcg tcctttcgac gtttatctcc acgatgaccg cccctttcga
     2581 tttcgcgcga tagggtatgt cggcggcggg gtagaccacc cccgacgtgc ctatgatgaa
     2641 catgaccttg cacctgtccg cctcttcatt ggccctggcc atttccatca tgggaatgcc
     2701 ctcgccgaag aaaacgacat cgggcttcag ggccgcgttg cacttttcgc attgaggata
     2761 ggggttgacg gtggtggcgt cgggcgtgaa gggcaggcgc gtgtggcagt tcatgcatac
     2821 gagccacctg tgattaccgt ggtattcgat gacgttgcta ttgccggcct cctggtggag
     2881 accgtccacg ttctgggtga tgacagcttt caggtagccc atcttctcga gcctgccgag
     2941 ggcgcggtga gcctcggagg gggccgaagc gaatatcacc tccgccatct cccgtagcat
     3001 cacccagacc ttctcgggcc gtctcacgaa ggagcttatc tcggcgtact ccatcgggtc
     3061 gtacttctcc cacagaccct gccctccgcg gaaggccggt attcccgcgt ccacggatat
     3121 cccggcgccg gtgaacgcca cgaggtattt ccgctccctg atgatatcgg caatcgcgct
     3181 gtagtcttcc attttaccct ccacctcagc ccgctttcca gcctttgttg atgtccctga
     3241 aggcggggat gatgccgaat ccatccgcct tcttgatgcc cctcttgatg gcctcggaaa
     3301 ggacgaattc ggcgagaacg ccgaccttgt tgatatccgc ttctatatcc cccatagaaa
     3361 gggcgaacac aaggtccccg tcgaaggtgg tatgcacagg attgatcgtc tttatgagac
     3421 ccccctgggc catctgggcg accttggtga tgtctctctt ggtgaacttc gcgttcgtgg
     3481 cgacaacgcc gagggtcgta ttcacgatcc cgaattgttt tttcacaatg ccctgcttca
     3541 ggcagttcgt tgtattggcg aattccagac tgtcctggga cttcctcaga ccggctatga
     3601 tcctgcccgt cacgttgtcg atgatatcgc cgaaggcgtt gacgacaacg agggcgccca
     3661 ccttaaggcc gtcgggcatg gcgacgctgc atgtccccag tccgcccttc atcgcccggg
     3721 acatctcgaa gagcttgccg acgaccgccc cggttccggc tcccacgctc ccctcctcga
     3781 cctcttcgcc ggcgttgagg caggcctcgt agcccatctg ggctgttgga cggatcctgt
     3841 gatcgccgaa gaggaggtcg aagatgacag ccgtggggac gatgggaatg cgggttatcc
     3901 ccacatcgaa gccgacgcct ttctcctcca ggtagcgcga cacgcccgag gccgcgtcga
     3961 ggccaaaaga gcttccgccg cacaggagga tcgcgtggac cctctcgacg atgtggccca
     4021 cgttcagcgc gtccacctgc cgtgtccctg aagcgctccc gcgaacatcg atcccggcaa
     4081 cggccccctc ttcacagagt atggcggtac accccgtccg gcccgtgagg tcggtggcgt
     4141 gtcccacctt gatgcccgga atatcagtta tggaattgaa catagacgat cacctttcaa
     4201 tgacgagatt gtgtcaatta cccaatttgt tccgaatatt cagggatttc tgctcgtttt
     4261 cccgcttgag gtccttcttc agctctatca gtgccttcgc aacggagtcg tatttgatgg
     4321 agagtatctc gcaggccagt atggccgcgt ttgtgccggc atcgatcccg acggaggcca
     4381 ccggaatacc cttgggcatc tgcacgatgg agtagagcgc atcctttccc ttgaggatgc
     4441 cgccgctggc cggaacgcct atgacgggca gtgtcgtgtg ggacgcaatg acgcccggaa
     4501 ggtgggcagc catcccgg
//
LOCUS       DAOQTR010000118         4513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21409_length_4513_cov_3.470615, whole genome shotgun sequence.
ACCESSION   DAOQTR010000118 DAOQTR010000000
VERSION     DAOQTR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21409_length_4513_cov_3.470615"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(695..1495)
                     /locus_tag="PKN85_04015"
     CDS             complement(695..1495)
                     /locus_tag="PKN85_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT43601.1"
                     /translation="MTIKRKIIHIDEERCNGCGQCVSACAEGAIELEGGKARLVAEKY
                     CDGLAACLGECPVGAISIIEREADPFDEEAVEEYLAGKKGAGDHAVEDPDGSVNPPSP
                     DPAGPTSLPCGCPSPQLQTFAPANSGTTDDGVGIRQATRLTHWPVQIRLVPPTAPFLR
                     NADILVASDCTPIAYPNFHEELLKGKVVLMGCPKFDDTDEYIDRFTAVFSAADIRSIT
                     IAIMEVPCCSKMPRIIEAALKRSGKKIPVETIVISPRGQIVQRTKLAA"
     gene            complement(1606..3240)
                     /gene="hcp"
                     /locus_tag="PKN85_04020"
                     /gene_synonym="priS"
     CDS             complement(1606..3240)
                     /gene="hcp"
                     /locus_tag="PKN85_04020"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNT43602.1"
                     /translation="MFCYQCEQTAKGEGCSKVGVCGKQPEVAALQDLLVYVTAEMSWF
                     ARQAGKHGIKSDRETNLLAAEALFHTLTNVDFDPERLKTKIAAVAAKRDDLAGKISAK
                     GGNTTLPGGKTPTRPDPTIEGLAKQGEAVGFQGAMRNENADVTSLMHTVIFGLRGVAA
                     YAYHAALLGKEDEAVYTFIYEALAEVIENRLGLNEWLGLVLKCGEINLKAMELLDAAN
                     TETYGHPVPTTVPLGAKKGKALLVSGHDLKDLEEILKQTEGTGIYVYTHGEMLPCHGY
                     PKLKKYPHFYGHYGTAWQNQHKEFAEFPGSIVMTTNCIQKPLESYAANIFTTGPVAWP
                     GVTHIADGNYRPAIDKALALPGFAADSNGKSVMVGFARNAIMGVADKVIEAVKAGKIR
                     HFFLVAGCDGAKPGRNYYTEFVEKVPDDCVVLTLACGKFRFFDKQLGDIGGIPRLLDV
                     GQCNDAYSAIQIAVALSKAFNVGVNELPLSLVLSWYEQKAVAILLTLLYLGIKNMRLG
                     PTLPAFITPNVLKVLVDNYSIKPISTPDQDLKEILG"
     gene            complement(3479..3718)
                     /gene="rpoZ"
                     /locus_tag="PKN85_04025"
     CDS             complement(3479..3718)
                     /gene="rpoZ"
                     /locus_tag="PKN85_04025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNT43603.1"
                     /translation="MARITVEDCMEMVENRFELVHLAARRSKQLIKGSKPLVKSTNRE
                     IVTSLREIAEKHIYFEKKESIETKETPYQLSTFTP"
     gene            complement(3699..4334)
                     /gene="gmk"
                     /locus_tag="PKN85_04030"
     CDS             complement(3699..4334)
                     /gene="gmk"
                     /locus_tag="PKN85_04030"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNT43604.1"
                     /translation="MSNSPDGRGPASTSEVQPDDPRKGVLIVVSGPSGVGKSTLIERF
                     LREDSHSEFSISYTTRQKRAHEIDGKDYHFVDEGTFESMVAKGHFLEWEKVHGNLYGT
                     PRAEILSILETGKDIVLDIDVKGAVNISGQCTAARLIFIEPPSVGDLINRLSLRGERE
                     IETRMKRVEEEIARKGEFGYTIVNDDLEKAYGTFRKVIDDIRREDNGTNNC"
     gene            complement(4324..>4513)
                     /locus_tag="PKN85_04035"
     CDS             complement(4324..>4513)
                     /locus_tag="PKN85_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HNT43605.1"
                     /translation="DNFRGTLASSEPIGRKLDFIIQEMVRETNTIGSKSNDLYINERV
                     IRIKVEIEKMREQVQNVE"
BASE COUNT          824 a         1268 c         1331 g         1090 t
ORIGIN      
        1 cgaagggtat aggcgggaac cccgtcggcc tgccccagag ggatctgctt ccaggctctt
       61 tttgccccct ccatcgtcat gggaaccttc tccaccttac tgagatcggt gatcttcata
      121 gtctcgctcc ttgtaggcat atgatgcaga atcagtcatt ataccacagc gcagcacccg
      181 gtcaaagtcc cgctgctcag accttgggcc aacagggggc agcgaagctc aatgccttat
      241 ctcatgcccg cttcgctaga gggcacagag gtgatgaggg gagcgcacag agagaagatc
      301 cggcctgatc ccggcgcccg ccggaatcag gccgtctttg catcccgtct ccgacgggag
      361 gaaaagaaga ggattcttct caggtggggg ggatgggagt tggggggcaa gggaggatgg
      421 ggtatagatc ctatgactac agggggggga tgagggggat gactttttct ggataaggaa
      481 aaagactatc tgtttctctc ccgccggaac ggcgggacga gaaggccgcc ggattttggc
      541 aggctccgaa atccggcggc aatcttttct ctcctttgcc ctctctttct aatctctgcg
      601 gactcgagcg aagcgggcgt gagataatgt ctttgagctt tcccccccgc tcttacctca
      661 cgaccggggc gacgaacggg cgtaagatgg gtctttaggc cgccagcttt gtcctctgca
      721 cgatctgtcc ccgggggctt atcacgatgg tttcgacggg tatttttttt ccggagcgct
      781 tcagtgccgc ctcgatgatg cggggcatct ttgagcagca ggggacttcc atgatggcta
      841 tggtgatgct ccgtatgtcg gcggctgaga agacagccgt gaatctgtcg atgtattcgt
      901 cggtgtcgtc gaacttcggg catcccatga ggacgacttt acccttgaga agttcctcgt
      961 gaaagttggg gtacgcgatg ggggtgcagt ccgaagcgac gaggatgtcg gcgtttcgga
     1021 gaaagggtgc cgtcggcggg acgagccgga tctggaccgg ccagtgggtg agccgcgtgg
     1081 cttgccggat gcccaccccg tcatccgtag tgccgctgtt ggcgggggcg aacgtctgaa
     1141 gctgggggga cgggcagccg cagggcaggg acgtgggtcc ggcggggtct ggggaggggg
     1201 ggttgacgga accgtcgggg tcttcgaccg cgtggtcgcc ggcacccttt ttccctgcaa
     1261 ggtattcttc gacggcctct tcatcgaagg ggtccgcctc tctttcgata atggatatgg
     1321 cgcccaccgg gcattcgccg agacaggcgg caagtccgtc acagtacttc tcggcaacga
     1381 gccgggcctt gcctccctca agctctatgg cgccttcggc gcaggcgctc acgcattgcc
     1441 cgcatccgtt gcatcgttct tcatctatgt ggattatctt tcgcttgatc gtcataagat
     1501 cctctcacgg gttatgggca atgggtcatg ggcgctctgc tttgatgctg tcccggttgg
     1561 tacttgctgt ccataaccca taacccatca cccgtaacct ctctcttatc ccaatatctc
     1621 cttcagatcc tggtctggcg tcgaaatggg ctttatgctg taattatcca cgaggacctt
     1681 gagcacgttc ggcgtgatga aggccggcag ggtgggtccc agccgcatgt tcttgatgcc
     1741 gagatacaga agggtgagca ggattgccac ggccttctgc tcataccagg agagcacgag
     1801 cgacaggggc agttcgttga cgcccacatt gaatgccttc gagagcgcca cggctatctg
     1861 gatggcggaa taggcgtcgt tgcactgccc gacgtcgaga agccggggga tgccgccgat
     1921 atcgccgagc tgcttgtcga agaaccggaa cttcccgcac gcaagggtga ggacgacgca
     1981 atcatcgggg accttctcga cgaactcggt gtaatagttc cttccgggct tcgcgccgtc
     2041 gcatcccgcg acaaggaaga aatgccgtat ctttccggcc ttgaccgcct ctatcacctt
     2101 gtccgccacg cccatgatgg cattgcgggc gaagccgacc ataacggact tcccattgct
     2161 gtcggcggcg aaaccaggca atgcgagggc tttgtcgatc gcgggtctgt aattcccgtc
     2221 ggcgatgtgg gtcactcccg gccaggcgac gggaccagtc gtgaatatgt tcgcggcgta
     2281 ggactccagt ggtttctgga tgcagttggt ggtcatgacg atggagccgg ggaattcggc
     2341 gaattccttg tgctggttct gccaggccgt cccgtaatga ccgtagaaat ggggatactt
     2401 cttgagcttc ggatatccgt ggcaggggag catctcgccg tgggtgtaga cgtatatccc
     2461 cgttccttcc gtttgtttga ggatctcttc gaggtccttg aggtcgtggc ccgagaccag
     2521 gagcgccttg cccttcttcg ctcccagggg gaccgtggtc gggacgggat gtccatacgt
     2581 ctcggtgttt gccgcgtcca gcagttccat ggccttgagg tttatctcgc cgcacttcag
     2641 gacgagcccc agccattcgt tgagccccag cctgttctcg atcacctccg cgagggcctc
     2701 gtagatgaag gtgtagaccg cttcgtcttc cttaccgagc agggccgcgt ggtacgcgta
     2761 cgccgcgact cccctgaggc cgaagatcac ggtgtgcatg agcgacgtga cgtccgcgtt
     2821 ctcgttgcgc atggccccct gaaaacccac ggcctcccct tgtttcgcga ggccttcgat
     2881 ggtggggtca ggtctcgtcg gtgtcttgcc gccgggcagg gtggtgtttc cacccttcgc
     2941 cgagatcttg cccgcaaggt cgtcgcgctt tgcagccacg gccgctatct tggtcttcag
     3001 ccgttccgga tcgaagtcga catttgtcag tgtatggaat agcgcctcag cggcgagcag
     3061 gttcgtttct ctgtccgact tgatcccgtg cttgccggcc tgacgggcga accatgacat
     3121 ctccgccgtc acgtagacca gcagatcttg aagagcggcc acctcaggct gcttcccgca
     3181 gaccccgacc ttcgaacatc cctcaccttt cgctgtttgt tcacactggt aacaaaacat
     3241 aagcccctcc tttatgtgga ttatgcgatc tcataaagcc aagtatagca ccgcgggaaa
     3301 aacgtgcttt gacttaggtc aagaagaggg gttatgggtg ataggtaatg ggttataggg
     3361 agaacgcggg agggacggca gacaatgaag ggagatgatg ggcaggggaa atcaagaaat
     3421 gggttacggg caaaattcct tacccataac ccattaccta taacccgctt ttacgcctct
     3481 agggtgtaaa cgttgacagt tggtagggag tttctttggt ctctatggat tctttctttt
     3541 cgaaatagat gtgtttctcg gcgatctccc tcaaggacgt gacgatctcc ctgttcgtcg
     3601 acttcaccaa tggtttcgat cccttgatga gttgttttga ccggcgcgcc gcgagatgga
     3661 caagttcgaa acggttctcc accatttcca tgcagtcttc aacagttatt cgtgccatta
     3721 tcctctctcc ttatgtcgtc gatcacttta cggaacgtcc cgtacgcctt ttcaagatcg
     3781 tcgttcacga tagtatagcc gaattcgcct tttcttgcaa tttcttcttc cacgcgtttc
     3841 atccgcgtct ctatctctct ctctccgcgc agggacaggc ggttgatgag atctcccacc
     3901 gaaggaggct cgatgaagat gagccgtgca gccgtgcact ggccgcttat attgaccgcg
     3961 cctttcacgt ctatgtcgag gacgatgtcc ttgcccgttt cgaggatgga caggatctcc
     4021 gcccggggtg tcccgtagag attcccgtgg accttttccc attcaagaaa atgccctttg
     4081 gcgaccatgg actcgaatgt tccctcatcg acaaaatggt agtccttgcc gtcgatctcg
     4141 tgcgcccgtt tctgccgtgt cgtgtaggag atggagaact cgctgtggct gtcttccctg
     4201 agaaaacgtt cgatgagagt cgacttgccc accccggacg gaccggagac aactatgaga
     4261 acgcccttcc tcgggtcatc gggctggacc tcggacgtgg acgccggccc tcttccgtcc
     4321 gggctattcg acattctgca cctgttcccg catcttctct atctccacct tgatccgtat
     4381 gacccgttcg tttatataga ggtcgttgga cttgctcccg atggtgtttg tctcccgtac
     4441 catctcctgg atgataaaat cgagttttct tcctatgggc tcggaggatg ccagggtgcc
     4501 gcggaagttg tcg
//
LOCUS       DAOQTR010000119         4490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21554_length_4490_cov_3.303269, whole genome shotgun sequence.
ACCESSION   DAOQTR010000119 DAOQTR010000000
VERSION     DAOQTR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21554_length_4490_cov_3.303269"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKN85_04040"
     CDS             complement(<1..679)
                     /locus_tag="PKN85_04040"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT43606.1"
                     /translation="MKRLILLPVLMMTLFLAAPCGAGQTVTIVSPEGRVSDREARHAL
                     ARILSYDDATLRESLAEYRALLSLFPGDTAIETETAQVLLRLGMEADAAALLRGLYAR
                     DPGDSTATAALADIECSRGHAVACRDLYLDALRTSRHRPDLALRFADKMNMWGDFARA
                     RTLYRQHLAANPGDRDVALRLAMTLASSERYEEAEGVCRGLMLTREDAAVVAALASIK
                     LLEKDFTA"
     gene            complement(681..1316)
                     /locus_tag="PKN85_04045"
     CDS             complement(681..1316)
                     /locus_tag="PKN85_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT43607.1"
                     /translation="MNRSPACLAAACLIAALLFLAGFECPFAAETSGRPDASPVEGQE
                     IPDWMARWELARVLSYAGRHEESISEYRKLLGEKPDLSKARIEMAKVLFRQKKGAEAL
                     GALEGIDARQLDADSVLLMADLYRDRKSYDRAAPLYREYLEKRPGDNAARLRLAEMLS
                     WEKQYDRSLAEYRKILAALPGDVQVRRKYAMVLMWAKRYGEAAAELRRTLK"
     gene            complement(1313..>1804)
                     /locus_tag="PKN85_04050"
     CDS             complement(1313..>1804)
                     /locus_tag="PKN85_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT43608.1"
                     /translation="DNLMKVLTFVISIIILALATTAAYRHLKQDWLLYRTAEDLSRRG
                     AWRQANPIFDDLVKKGFRTKDTLRLLGKSYEETGDLAAAIEAFRKLAAASPGDAGVEL
                     ELAGLYDRKGDHAGAAAIYHALLAKEPENRSVALRLARALTALGRYDEAILRYRAILG
                     EKP"
     assembly_gap    1805..1904
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1912..3672
                     /gene="aspS"
                     /locus_tag="PKN85_04055"
     CDS             1912..3672
                     /gene="aspS"
                     /locus_tag="PKN85_04055"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNT43609.1"
                     /translation="MNVRDTYCGTIRKEHVDMTVTLAGWVFRRRDHGGLVFADLRDVT
                     GIVQIVFSPEISKEAHERAGDIRSEYVLKATGKVRRRPAETENPALPTGEIEVYVSDF
                     EVLNTCKVLPFQLDDEDIGEATRLRYRYLDMRRPEMQKVFIERSKAYTVTRDYLLSKG
                     FYEFETPLLTKSTPEGARDFVVPSRLNTGMFYALPQSPQLFKQLLMMSGFERYFQIAR
                     CFRDEDLRADRQPEFTQIDIEMSFVDREDVMNVNEGLVIALYEALKGRKPPEVPFPRM
                     SFAEAMEKYGSDKPDLRFDLPIVDMTHIFQKTEFGVFKKTIEAGGAVKGLVLKGKTLS
                     RKDLDVSVETARDIGAGGLVWIRMDADRALQSPTVKFLSDEEKSQMISLFSIEPGDVL
                     FIMSDKRLRVSELMGRFRLYLGEKYDLIDKSLDKFLWVIDFPLLEYNEEEKRYVARHH
                     PFTSPKGSLAGFDGDYDSILANSYDLVFNGVELGGGSIRNHRTEDQAEMFRLLNIGEE
                     EAADKFGFLLEALEMGAPPHGGIAFGFDRILMMFLGLGSIRDVIAFPKTQKGSCLLTG
                     APGRVGPRQLAELKIRTIAD"
     gene            3691..>4490
                     /locus_tag="PKN85_04060"
     CDS             3691..>4490
                     /locus_tag="PKN85_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43610.1"
                     /translation="MKKKEKKKGFIFKNVAGASNYPLIDVDHPQLFRDIFPYDEVCKV
                     KFDHKIELIDPPDDIYITDTTFRDGQQARPPFTAQQIEDLFDFLHRLSGPNGVIRQSE
                     FFLYTDKDKEALRRCQEKGYRFPEITGWIRAHKEDLQLVKDMGLKETGILTSVSDYHI
                     FLKLNKTRSQVFKDYLKIVESALDHGIIPRCHFEDVTRADIYGFCVPFAQALMALSEE
                     ARLPVKIRLCDTLGVGITYPGSALPRSIGKIIRAMIDATPVFWDFPMC"
BASE COUNT          886 a         1285 c         1344 g          875 t
ORIGIN      
        1 ccgcggtgaa gtccttttcg aggagtttta tcgaggcaag ggccgcgaca actgcggcgt
       61 cctcgcgggt aagcatgagg cccctgcaga cgccttcggc ctcttcgtag cgctccgaac
      121 tggcaagggt catcgctagc ctcaaggcca cgtcacggtc gccggggttg gcggcaagat
      181 gttgcctgta gagggtccgg gcccttgcga aatctcccca catgttcatc ttatcggcga
      241 aacgtagcgc gaggtccggc ctgtgtcgcg atgtccgcag cgcgtcgaga taaaggtccc
      301 tgcaggcgac cgcatggccg cggctgcatt cgatatcggc aagggctgca gttgcggtgc
      361 tgtcgccggg gtcccgggcg tagaggccgc gaaggagcgc ggcggcgtcg gcctccatcc
      421 ccagacgcag gaggacctgt gccgtttccg tttctatggc ggtgtccccg gggaagagtg
      481 acaggagggc gcgatactcc gcaagggact ccctcagcgt ggcgtcgtcg taggagagga
      541 tccgcgccag ggcgtggcgg gcttccctgt cggataccct gccttccggg gacacgatgg
      601 tgaccgtctg tccggccccg cacggtgccg cgaggaacag ggtcatcatg aggaccggta
      661 gaagaatgag cctcttcatg ctatttgagc gttctcctca gttccgctgc ggcttcaccg
      721 tatctcttcg cccacatgag gaccatcgcg tattttctcc tcacctgcac atcgcccgga
      781 agcgcggcaa gtatcttccg gtattcggcg agggatcggt cgtattgctt ctcccagctc
      841 agcatttccg ccagcctcag cctggccgcg ttgtcaccgg gacgcttctc aaggtattcc
      901 ctgtaaagcg gggcggccct gtcgtaagac tttcggtccc tgtaaaggtc ggccatgagc
      961 aggacagaat cggcatcgag ctggcgggcg tctatgccct cgagggcgcc aagtgcctct
     1021 gcgcccttct tctgccggaa aaggaccttt gccatctcga tccttgcttt cgaaaggtcc
     1081 ggcttctccc ccaggagctt cctgtattcg ctgatggatt cttcatggcg cccggcgtag
     1141 ctgaggaccc gggcgagctc ccatctcgcc atccagtcag ggatctcctg gccctcgaca
     1201 ggtgaggcat cgggccgccc ggatgtttcg gcggcaaagg ggcattcaaa ccccgcaagg
     1261 aacaggagtg ctgcgataag gcatgctgcc gcgagacaag ccggtgatcg gttcatggtt
     1321 tctctcccag gatggccctg tagcgcagga tggcctcgtc atagcgtccc agggcggtca
     1381 gggcccgggc tagccgcagg gcgacggatc tgttttcggg ttccttcgca aggagggcat
     1441 ggtaaatggc cgccgcaccc gcgtgatcgc ccttccggtc atacaatccg gccagttcaa
     1501 gttccacccc ggcgtcgccg gggcttgcgg ccgccagttt cctgaaggcc tctatggccg
     1561 ccgccagatc gcctgtttcc tcgtaggact tcccgagaag cctcagggtg tcttttgtcc
     1621 ggaagccctt ctttaccaga tcgtcaaaga tcgggttcgc ctgccgccag gcccctcttc
     1681 gggacaggtc ctccgccgtt ctgtagagca gccagtcctg cttcagatgc cggtacgccg
     1741 ccgtcgtggc cagggcaagg atgatgatcg atatgacaaa ggtaaggact ttcataaggt
     1801 tatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaaggg gctgaacgtg
     1921 agagatacct attgcggcac gatacgaaaa gaacatgtgg atatgacagt gaccctggca
     1981 ggatgggtct tcaggagacg cgaccacgga gggcttgtat tcgccgatct gcgcgatgtg
     2041 acggggatag tccagatcgt gttctctccc gagatatcga aggaggcaca cgagagggcc
     2101 ggcgacatac gcagtgaata cgtactcaag gcgacgggca aggtcaggag gaggcccgcg
     2161 gagacggaga acccggccct gcccacggga gagatcgagg tgtatgtctc ggacttcgag
     2221 gttctcaaca cctgcaaggt ccttcccttc cagctcgatg acgaggacat aggcgaggcc
     2281 acgcgtctca gataccgcta tctcgacatg cggcgcccgg agatgcagaa ggtcttcatc
     2341 gagaggagca aggcctacac ggtcacgagg gactacctgt tgtcgaaggg gttctacgag
     2401 ttcgaaacac cactcctcac aaagtcgacg cccgaaggcg cgcgcgattt cgtggtgccc
     2461 agcaggctga acacgggcat gttctacgcc ctgccgcagt cgccccagct cttcaagcag
     2521 ctcctcatga tgagcggctt cgagagatat ttccagatcg cccgttgttt tcgcgacgag
     2581 gacttgaggg ccgaccgtca gccggagttc acccagatag atatcgagat gagctttgtc
     2641 gacagggaag acgtgatgaa cgtcaacgaa ggtcttgtca ttgcccttta cgaggcattg
     2701 aaaggcagga agccacccga ggtccctttc ccccggatga gcttcgccga ggcgatggag
     2761 aagtacggca gcgacaagcc cgacctgcgc ttcgatctgc ccatcgtcga catgacacac
     2821 atattccaga agacggagtt cggggtcttc aagaagacga tagaggccgg cggggccgtc
     2881 aagggcctgg tcctcaaggg gaagacgctc tcccgtaagg acctcgacgt gtcggtggaa
     2941 acggccaggg atataggcgc cggcggtctc gtctggatac gcatggacgc cgacagggcg
     3001 cttcaatctc cgacggtgaa gttcctgagc gacgaagaga agtcgcagat gatatccctt
     3061 ttcagcatcg aacccggcga tgttctgttc atcatgtccg acaagcggct ccgggtgagc
     3121 gagctcatgg gaaggttccg gctctacctg ggcgagaaat acgatctcat cgacaagagc
     3181 ctggacaagt tcctctgggt catcgatttc ccccttctcg agtacaacga ggaggagaag
     3241 cgctacgttg cccgccacca tccctttaca tctcccaaag ggagcctcgc cggtttcgac
     3301 ggagactacg attccattct cgccaattcc tatgacctcg tcttcaacgg tgtcgagctg
     3361 ggcgggggga gcatcaggaa ccatcggacg gaggaccagg cggagatgtt ccggctcctc
     3421 aacataggcg aagaggaggc cgctgacaaa ttcggctttc tcctggaggc ccttgagatg
     3481 ggggcgcctc cccatggcgg catagccttc ggtttcgacc gcatactgat gatgttcctg
     3541 gggcttggca gcataaggga cgttatcgcc tttccgaaga cgcaaaaggg ttcctgcctt
     3601 ctgaccggcg cacccggtag ggtggggccg agacagcttg cggagctgaa gatacggacc
     3661 attgccgatt gacatcttta cgggggtgac atgaagaaga aggagaagaa gaagggattt
     3721 atctttaaga acgttgccgg tgcgtccaac taccctctca tcgacgtgga ccaccctcag
     3781 ctcttcaggg acatctttcc ctatgatgag gtctgcaagg tgaagttcga ccacaagatc
     3841 gagctcatcg accctcccga cgatatctat attacggaca cgaccttcag ggacggccag
     3901 caggcaaggc ctcccttcac ggcgcagcag atagaggacc tcttcgactt tctccaccgg
     3961 ctgagcggac ccaacggggt catcaggcaa tcggagttct tcctttacac ggacaaggac
     4021 aaggaggcac tgcgcaggtg ccaggagaaa ggctaccgtt ttccggagat tacgggatgg
     4081 atacgggcgc acaaggagga cctccagctc gtcaaggaca tggggctcaa ggagacaggc
     4141 atcctgacaa gcgtctccga ctaccacata ttcctgaagc tcaacaagac gaggtcccag
     4201 gtcttcaagg actatctgaa gatagtggag agcgctctcg accacggcat aataccgaga
     4261 tgtcacttcg aggatgtgac gagggctgat atctatggtt tttgcgttcc cttcgcccag
     4321 gcgctcatgg ccctgtcgga ggaggcgagg ctgcccgtaa agatacggct ctgcgacacc
     4381 cttggcgttg gcatcacgta ccccggttcg gcgcttcccc ggtccatcgg gaagatcatc
     4441 cgcgccatga tcgacgccac cccggtgttc tgggacttcc cgatgtgcga
//
LOCUS       DAOQTR010000120         4473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_21687_length_4473_cov_3.499321, whole genome shotgun sequence.
ACCESSION   DAOQTR010000120 DAOQTR010000000
VERSION     DAOQTR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21687_length_4473_cov_3.499321"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PKN85_04065"
     CDS             complement(<1..1065)
                     /locus_tag="PKN85_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNT43611.1"
                     /translation="MASELVINVTYSETRIAFLENGTLVEFFIEKKNDHSMVGSIYKG
                     KVVRIVPGMDAAFIDIGLEKSAFLYVGDIILDRMMYEEFDSSDFHFEAGERIEGVLED
                     GQELIVQVSREPIGQKGTRVTSKITLPGRLLVLMPGTDHIGVSRKIEDEEERKRLATV
                     MREICPKGFGLIGRTASEGKTADELATDLNFLKRIWESIQEKAKATRAPAILHQELGI
                     IFRVIRDLHSHSLKRIIVDDQYIHSRLEGFLKEYLPEEGCEVIHFDERDPIFEVYGIE
                     MEVAKLLQKKIWLKSGGYIVLDYTEALTVIDVNTGRYLGKKDLEDTILRTNLEAVKEI
                     AYQIRLRNVGGIIIVDFIDME"
     gene            complement(1055..3310)
                     /locus_tag="PKN85_04070"
     CDS             complement(1055..3310)
                     /locus_tag="PKN85_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNT43612.1"
                     /translation="MRPARYIGCEPNHVRKDPGDVTVRFALCYPDIYEIGMSYYGLFL
                     LYEIANNVQGVWCERCFAPWADMEEHLRRSGTPLGTLESRTPLHAMDLIGFSLTYELN
                     VTNVLNMLALGGVKIRAEERTGKDPIVIGGGPLMLNPKPYERFFDIIVAGEGDEILRS
                     LLETARDMKGEPRDSIIREMARLEGVYSSRVPSPQVKRLFISDLDSAYHPVRPPIPTV
                     DSVHNRLNIEISRGCGNGCRFCLAGFGYRPYRERSFEAVRAVIDEGLRNTGYEEISLL
                     SLSSGDYPFLFDVLEYARSTHRGLSVSLPSLKIGSIGKDEISAMGEMARTGFTFALEA
                     PTASLRSRLNKDIDVETLVAQLPHLRALGWRRLKLYLMVGFPWETDDDLLAIRDVITP
                     FRAAGMDVNLSVSPFTPKPHTPFQWLPMDEEGVLAEKIMLIKDALKKTGVRVRYRDTS
                     VSVVEGIVARADERLASLFEYLHDRGVRLEAWREFFSFEPYRTWFEENSADMRAYTGS
                     RDRAVGSSWDIVDMGFDEAFFGTELDKAESGEMTVSCLAGCAACGLGCSLPQRTPRQE
                     KPEGIVVSDAASPAAEAAGAPKKFTFRYGKYGDGRYIGHLDVMNILVRAMKSAGITMR
                     TRGKYHPLPKIALTDALPVGVESFTEFIEVETGSAQQVDASTVAMINEKLPSGIKIYE
                     FIEGSLRDMVKEYVFLLSADAPVEDVPTLWRRGKDRFFYIWRGKGVKQLWMQGSYTRI
                     IKVESRRIDGF"
     gene            complement(3436..>4473)
                     /locus_tag="PKN85_04075"
     CDS             complement(3436..>4473)
                     /locus_tag="PKN85_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNT43613.1"
                     /translation="GFKVAVDRATLYGDRIQDIRRIIESGEYVAARGSYREYKDIVRD
                     YHEYLRGNINLNKGFNVVMDAGNGTGGVIAAPIMKEFGQKVTELFCDMDGHFPNHFPD
                     PTVEKNLVTLRDTVRKEGADVGIGYDGDADRIGVVDEKGGIIWGDYLMLIYARQILKE
                     HNGATFVSEVKCSRNLFDDIAKRGGKPIMWKAGHSLIKQKMKDVHALMGGEMSGHIFF
                     ADRFFGFDDAIYASLRFLEIMEQDGRPVSEFLADLPKTYFTPEIRTDCPDSVKFDVVR
                     ELTELYRSRYPIIDIDGVRAIFDDGWGLVRPSNTQPILVLRFEAETEEALDRIQSMVM
                     EDLERIMERYR"
BASE COUNT          865 a         1373 c         1243 g          992 t
ORIGIN      
        1 ctccatgtcg atgaagtcga cgatgatgat gccacccacg tttcgcagcc gtatctggta
       61 agctatctcc ttgaccgcct ccaggttggt cctcaggatg gtgtcttcca ggtccttctt
      121 gccgagatac cgtcccgtgt tcacatcaat gacggtgagg gcttcggtat agtcgagaac
      181 tatgtacccc cccgacttca gccatatctt tttctggaga agcttggcca cctccatctc
      241 gatgccatag acctcgaata tggggtcacg ctcatcgaaa tggatcacct cgcagccctc
      301 ctcgggaagg tattccttga gaaacccctc caggcggctg tggatgtact gatcgtcgac
      361 tatgatcctc ttgaggctgt gggaatggag atcgcggatg acccggaaga tgatgccgag
      421 ctcctggtgg aggatggccg gcgcgcgtgt ggcctttgcc ttttcctgga tgctctccca
      481 gatgcgcttc aggaagttga ggtccgtggc gagttcgtcc gccgtcttgc cctccgacgc
      541 cgtcctgcct atcaacccga accccttcgg gcatatctcc ctcatcaccg tggcaaggcg
      601 cttgcgttcc tcctcatcct cgatcttccg ggagacgccg atgtggtccg tgccgggcat
      661 gaggaccagg agccgtccgg gaagggtgat cttcgatgtg acacgcgtcc ccttctgccc
      721 gataggctcg cgggagacct gaacgataag ctcctgcccg tcctcgagga ccccttcgat
      781 cctttctccc gcttcgaagt gaaaatcgga gctgtcaaat tcctcgtaca tcatgcggtc
      841 gaggatgatg tcgcccacgt agaggaaggc cgacttctcg agtccgatgt ctatgaatgc
      901 ggcatccatg ccgggcacga tcctcacgac cttgcccttg tagatgctcc ccaccatgct
      961 gtgatcgttc ttcttctcga tgaaaaattc tacgagggtc ccgttttcca ggaaagcgat
     1021 ccttgtttcg ctatacgtta cgttgattac aagttcagaa gccatcgatc cgtctcgatt
     1081 ccacctttat gatcctcgtg taactaccct gcatccagag ttgcttcacg cccttgccgc
     1141 gccaaatgta gaaaaacctg tccttccccc gcctccagag agtgggtaca tcttccactg
     1201 gagcatcagc actgagaagg aagacgtatt cttttaccat atctcttaga gaaccttcaa
     1261 taaattcgta tatttttatg cccgaaggca gtttttcgtt gatcatcgcc accgttgacg
     1321 cgtccacctg ctgggcgctg cccgtttcga cctcgatgaa ttcggtgaag ctttccacac
     1381 ccaccggaag ggcgtccgtg agagctatct tcggcagggg gtgatacttg ccccgtgtcc
     1441 tcatcgtgat acccgcggac ttcatggccc ttacgagaat gttcatcacg tcgagatggc
     1501 cgatgtagcg gccgtcgccg tacttgccgt agcgaaaggt gaacttcttc ggagcaccag
     1561 cggcctcagc ggccggcgag gcagcgtccg aaacaacgat gccctcgggc ttctcctgac
     1621 ggggcgtccg ttgcggaaga gaacatccaa ggccgcaggc ggcgcagccg gcgaggcacg
     1681 acacggtcat ctcgccagac tccgctttgt ccagttccgt cccgaagaag gcctcatcaa
     1741 aacccatgtc gacgatatcc caggaggatc cgaccgctct gtcgcggctg ccggtatacg
     1801 ccctcatgtc cgccgaattc tcctcgaacc aggtccggta aggttcgaaa gagaagaact
     1861 ccctccacgc ctcgagacgc accccccggt catgaagata ctcgaagagg gatgcgagac
     1921 gctcgtccgc cctcgctacg atgccctcca cgacactcac cgaggtgtcg cggtagcgca
     1981 cccgaacgcc cgtcttcttc aaggcgtcct tgatgagcat gatcttctcc gcgagaacac
     2041 cctcttcgtc catggggagc cactggaaag gcgtatgcgg tttcggcgta aaaggggaca
     2101 cggagaggtt cacgtccatg cctgccgcgc gaaacggggt tatcacgtcc cggattgcca
     2161 ggaggtcgtc gtcggtctcc cagggaaaac cgaccatgag gtagagcttg agcctccgcc
     2221 agcccagggc cctgagatgc ggaagctgag ctacaagggt ctccacatcg atatccttgt
     2281 tgaggcggga ccggagtgag gccgtcggcg cctcgagggc gaaggtaaac cccgtgcgcg
     2341 ccatctcccc catggcggat atctcgtcct ttccgatgct gcctatcttg agcgaaggca
     2401 ggctcacgga gagcccacgg tgggtgctcc tcgcgtattc cagcacatcg aagagaaagg
     2461 ggtaatcccc cgagctcagc gacagcaggg agatctcctc gtacccggta ttccggagcc
     2521 cctcatcgat cacggccctg accgcctcga aggagcgctc gcggtaaggc cggtagccga
     2581 aaccggccag gcaaaacctg cagccgttgc cgcagccccg cgatatctcg atgttgagac
     2641 ggttgtgcac gctgtccacc gtgggtatcg gggggcgcac cgggtggtac gcgctgtcga
     2701 gatcgcttat gaagagacgt ttcacctgcg gagaaggaac acgggaggaa taaacccctt
     2761 ccagccgggc catctcccgg atgatgctgt cccgcggctc tcccttcatg tcgcgcgccg
     2821 tttcgaggag ggaccggagt atttcatcgc cttctccggc cacgatgata tcgaagaaac
     2881 gttcgtacgg cttcggattg agcatgagtg gaccgccccc gatgacgatg gggtcctttc
     2941 ccgtccgctc ttcggcccgt atcttcaccc ccccgagggc gagcatgttg aggacgttgg
     3001 tcacattgag ctcgtaggtc agagagaacc ctatgaggtc catcgcgtga aggggcgtcc
     3061 tcgactccag cgtcccgagg ggggttcccg atctcctgag gtgttcctcc atgtccgccc
     3121 agggggcgaa acagcgctcg caccagaccc cctgaacgtt gttggcgatc tcgtagagga
     3181 gaaagagccc gtaataggac atccctatct cgtatatgtc ggggtagcac agggcaaacc
     3241 tgaccgtcac atcgccgggg tccttcctca catggttcgg ctcgcacccg atataccgcg
     3301 cgggcctcat aatatttcgg ggaatatcga tcatcagtct aaaggcgggt catgggtcat
     3361 gggtcatggg tcatgggaaa aacagaacag ccggatggta ttcttctttt cctatcaccc
     3421 attacctatt acccatcacc tgtatctctc cattatcctt tccaggtctt ccatgaccat
     3481 cgactggatg cggtcgaggg cctcttcggt ttcggcctcg aacctcagga cgaggatggg
     3541 ctgcgtgttg gagggacgca cgagtcccca gccgtcgtcg aatatggccc gcactccgtc
     3601 gatgtctatg atgggatacc gggaccggta gagttcggtc agctcgcgca cgacatcgaa
     3661 cttcacactg tccgggcagt cggtgcgtat ctccggtgta aaataggtct tcggcaggtc
     3721 ggcgagaaac tccgatacgg gcctgccgtc ctgttccatt atctcgagga accgaagaga
     3781 ggcgtagatc gcgtcatcga atccgaagaa acggtcggcg aagaagatgt ggccgctcat
     3841 ctcgccgccc atcagggcgt ggacatcctt catcttctgc ttgatgagcg aatggcccgc
     3901 cttccacatg atgggtttgc cgccgcgctt cgcgatatcg tcgaaaaggt tccgggaaca
     3961 cttaacctct gagacgaatg tcgccccatt gtgttctttg aggatctgcc gggcgtagat
     4021 gagcatgagg taatcgcccc agatgatgcc gcctttttca tccacgacac ctatccggtc
     4081 cgcgtcgcca tcgtacccga tgccgacgtc cgcgccttcc ttgcggacgg tgtccctcag
     4141 tgtgacgagg ttcttctcca cggtagggtc ggggaaatgg ttggggaaat gcccgtccat
     4201 gtcgcagaac aattccgtca ccttctggcc gaactctttc atgatcgggg ctgcgatgac
     4261 ccctcccgtg ccgtttccgg cgtccatgac gacgttgaag cccttgttca gattgatgtt
     4321 cccgcgcagg tactcgtggt aatccctgac gatgtccttg tactcgcggt acgatccccg
     4381 tgccgcgacg tactcccccg actcgatgat cctccttatg tcctgtatcc ggtctccata
     4441 gagggtcgcc ctgtccacgg ctaccttgaa acc
//
LOCUS       DAOQTR010000121         4413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22085_length_4413_cov_6.409592, whole genome shotgun sequence.
ACCESSION   DAOQTR010000121 DAOQTR010000000
VERSION     DAOQTR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22085_length_4413_cov_6.409592"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..790)
                     /locus_tag="PKN85_04080"
     CDS             complement(11..790)
                     /locus_tag="PKN85_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNT43614.1"
                     /translation="MRFRLRPVLLFLSIFLVLTSPTLAGPLEDCAEYARLGVPGQDGD
                     LLCRTGHLLAHDPIRKTPIWVAERVTAEKATSTVVPREAGSFRIDPGLKKGRRAGLSD
                     YRKSGYDRGHMAPAADMHWDKQAMIECFYLSNMVPQVGKKMNQGIWKALEELVRAWAI
                     DRGELFIYTGPIYDGATTKTIGKNKVAVPSRLYKIVYDPNKQEAIAFIMPNEALETED
                     LPLYIFTIRDVEESTGLDFLSSLDKELQDSIETTRAAGLWQ"
     gene            complement(1201..2676)
                     /locus_tag="PKN85_04085"
     CDS             complement(1201..2676)
                     /locus_tag="PKN85_04085"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNT43615.1"
                     /translation="MIPIEHILLIGSLLILVSIGVARLSENLGVPALLLFLGIGMLAG
                     SEGPGGIYFDDAPLAQSVGVIALVFILFAGGLDTKWSEVKISFFPALSLATVGVLLTA
                     TGVGLFAFFALKLPFLTALLLGAVVSSTDAAAVFSVLRSKNISLKGRLRPLLELESGS
                     NDPMAVLLTIGIIQIIVNPGASFGSIALLFVQQMGLGVVFGFFFGRATVILLNRLKSY
                     YEGVYPVFFLALAVLTYGLTAAAGGSGFLAVYIAGLIAGNSDFIYKKSLLRFFDGLAW
                     LSQIAMFLTLGLLVFPSRIVPVVWAGLACSAFLMFVARPAGVFLSLLFARLQWREKVL
                     VSWVGLRGSVPIVLATFPLLAGVAGSETIFNIVFFIVLTSTLLQGWSIPVVARLMKLQ
                     APLEMKRHYPIEFAPIDGVDTQLVDFIIPFNAAIAGKAIVELGLPPDSLIVLVNRDEK
                     FFVPSGGTVLEGGDVVLVLVNDRNISEVREKLMRWKEPVKE"
     gene            complement(2727..3605)
                     /locus_tag="PKN85_04090"
     CDS             complement(2727..3605)
                     /locus_tag="PKN85_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT43616.1"
                     /translation="MTRIRSILAATDFSPNSRHAAERTAMLGATLGVPRGLLLHVLEE
                     SWLESLKRFIGSPAEVERGIIDDASSSLVELAEEIRQASGFSFETQVRKGSTLDGIAE
                     AASHHDLLVVGAHGRHPVRTLALGTTAQRLLGKTRTPVLVVKGKPDRPYRRILVAVDF
                     SPNSQMAMEGCSAIAPEALVRVVHVYEPLLERQMISAGASDKVLEEYRAKARIDAETQ
                     MTAFLEAGQWSTGRTLQHVEYGHASARLPEIAHEWGPDLVVVGKHGRSRVEELLLGSV
                     TIHLLSQSRCDVLVAQ"
     gene            complement(3851..4252)
                     /locus_tag="PKN85_04095"
     CDS             complement(3851..4252)
                     /locus_tag="PKN85_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNT43617.1"
                     /translation="MMNMVHFLVGDSPRRPYYAVLCFIVVAFAFSFFYLAILPGVQGL
                     PSLSHTGQGKSATQAVGFLDCLYFSIGTQTTLGYGDLIPATASAKIASMVQAAFGYVY
                     LAFLVSVFTAQAVLRSRRFQTYLREMIRTLR"
BASE COUNT         1002 a         1241 c         1288 g          882 t
ORIGIN      
        1 aatccctttc ctattgccag agaccggcgg ccctggtggt ctctatgctg tcctgcagtt
       61 ccttatccaa gctcgagaga aagtcgagcc cggtgctctc ctcaacgtcc cgtatggtga
      121 agatatacag gggcagatcc tctgtctcaa gggcctcatt cggcataatg aaggcgatgg
      181 cctcctgctt gttcgggtca tagacgatct tgtaaaggcg ggagggaacc gcaactttgt
      241 tcttcccgat ggtcttcgtg gtggcgccat catatatggg accggtatag atgaagagtt
      301 ctcctctgtc tatcgcccag gcgcggacga gttcttcgag ggccttccaa atcccctgat
      361 tcatcttctt gcccacctgg gggaccatgt tcgagaggta gaagcactcg atcatcgcct
      421 gcttgtccca gtgcatgtcg gcggccggcg ccatgtgtcc gcggtcgtaa ccgctcttcc
      481 ggtagtcaga aagccctgcc cgtctcccct tcttcaagcc gggatcaatt cggaacgacc
      541 ccgcttcccg cggcaccacc gtgctcgtgg ccttttccgc ggtgacgcgc tccgcgaccc
      601 agatgggagt cttacggatg gggtcatggg caaggaggtg gccggtacga cagaggaggt
      661 ctccatcctg gccaggaacg ccaagccggg cgtattccgc acagtcctcg aggggaccgg
      721 cgagggttgg ggatgtgagg acaaggaaga tggaaaggaa aagcagaacg gggcgtagcc
      781 tgaaacgcat ggaggcctcc tgatacgaac gggactgccg atgcgtctat gaaattgtga
      841 aattactggc ccctgtattg atcatattgt ctgggatatg ggggaggttt gcaaggggaa
      901 agtataaaaa gccccgatct ggtccatgac taccagaagg ctggtttctc ctggggtgag
      961 agggaccatt cgaggccttt gaggctaccc tgtgcatcgc gcctgcagat gctggggcgc
     1021 aaatagctct tggaaagttt agcagggatc cgcgccttgt caagggcgtc ggcaattgtt
     1081 gagttggccg gtacagtaga ccggtacggt agttggccgg tacacctcaa atatctcttc
     1141 tgccaacatg aatgcaaggc ctttcgaagt catcgccccg tgcaagcacc tgccggtccg
     1201 tcattccttc acgggctctt tccacctcat gagtttctcc cggacctcgc ttatgttcct
     1261 gtcgttgacc aggaccagga ccacatcccc tccttcgaga acggtaccgc cgctgggcac
     1321 aaagaacttc tcgtcgcggt tcacaagaac gatgaggctg tcgggcggaa gaccgagttc
     1381 cacgattgcc ttgccggcga tcgctgcatt gaagggaatg atgaagtcga cgagttgagt
     1441 gtcgacaccg tcgatgggag caaattcgat ggggtaatgt cttttcattt caaggggcgc
     1501 ctgcagcttc atcaggcggg caacgacggg gatcgaccag ccctggagca gggttgaggt
     1561 gagcacgatg aagaagacga tgttgaatat cgtctcggag ccggccacac ctgccaaaag
     1621 agggaaggtc gcgaggacaa tgggcacgga ccctctcagg cccacccagg aaacgaggac
     1681 cttttccctc cattgaaggc gggcaaagag gagggaaagg aagactcccg cgggacgggc
     1741 aacgaacatg aggaatgccg aacaggcaag gccggcccaa acgacgggga cgatacggga
     1801 tggaaacacc aggaggccga gggtgaggaa catggcgatc tggctgagcc aggcaaggcc
     1861 gtcgaagaag cgcagcaggc tcttcttata gatgaagtca ctgttgcccg caatcaggcc
     1921 agcaatgtac acggcaagaa agccgcttcc ccccgcggcc gccgtcaagc cgtaggtgag
     1981 cacggctagg gcaaggaaga aaacaggata gacgccttcg taataggact tcaatcgatt
     2041 gagcaatatg accgtcgctc ttccgaagaa gaacccgaag acgaccccga gccccatctg
     2101 ctggacaaac aggagggcga tggacccgaa cgaggcacca gggttcacta tgatctgaat
     2161 gatccctatg gtgaggagga ccgccatggg atcgttgctg cccgattcaa gttcgagcag
     2221 gggtctcagg cggcccttga ggctgatatt cttggaccgg agaacggaaa agaccgccgc
     2281 ggcgtcagtc gatgacacaa cagcccccag gagcagggcg gtgaggaacg ggagcttcag
     2341 ggcaaagaag gcgaagaggc cgaccccggt cgccgtgagc aggactccga cggtcgccag
     2401 gctcagtgcc gggaagaagg atatcttgac ctccgaccac tttgtgtcga gacctcccgc
     2461 gaaaaggatg aatacgagtg caatgacacc aacggattgc gcgagggggg catcgtcgaa
     2521 gtagatcccg ccgggacctt cggaaccggc aagcatccct atgccgagaa acaagagcag
     2581 agccggcacc ccaaggttct cggatagcct cgcgaccccg atactgacca ggataaggag
     2641 cgaacctata aggagaatgt gttcgattgg tatcatacgg caatcgtctc agctttatcc
     2701 aaaaccatat gctatcaccg cgaagactat tgagcaacga gcacgtcgca tcgcgactgg
     2761 gacaacaggt gtatcgtcac gctgccaagc aggagttctt caacgcgtga cctcccgtgc
     2821 ttgcccacga ccacgagatc gggaccccac tcgtgagcta tctccggcag tctcgctgac
     2881 gcatgtccgt attcaacgtg ttgcagcgtt cttcctgtgc tccactgccc ggcttccagg
     2941 aaggcggtca tctgtgtctc ggcgtcgatc cgtgcttttg cccgatattc ttcgagaacc
     3001 ttgtcggatg ccccggcgga gatcatctgc ctttcgagga gcggctcgta gacgtgcacc
     3061 accctgacga gggcttcggg ggctatcgcg gaacaaccct ccatggccat ttgggaattc
     3121 ggtgagaaat cgacggcaac gaggatcctc cgatacggcc tgtcaggctt gcccttaaca
     3181 acaaggacag gtgtacgcgt tttgcccaga aggcgctggg cggtagtgcc aagggccagg
     3241 gtccgtaccg ggtgccgacc gtgagcaccc accacgagca ggtcgtgatg tgaagcagcc
     3301 tcggcgatcc cgtccagagt actgcccttg cggacctgtg tctcgaagga aaagcccgat
     3361 gcctgtcgga tctcttcggc cagttccacg agggaactcg aagcatcatc gatgattcca
     3421 cgttcgacct cggccggcga cccgatgaac cgcttcagac tctccagcca tgattcctcc
     3481 aaaacgtgga gcagcaatcc tctcggcacg cccaacgttg caccgagcat tgccgtacgt
     3541 tccgcggcat gccgggaatt gggagaaaag tcggttgcag ccagaataga tcggatacgt
     3601 gtcatgttct aatcctcccg tgtctgagat gcacagcgcc ggtcagaggg tgtggcaggg
     3661 ttctggcgcg attgtcgaag tccacagccc ccccacctca tgctccgacc tctgattcgt
     3721 tacagatgag accggcttag gccatgagct catcatcagg ctatccgcca tggcttgcgt
     3781 gaatcatgcc atcattcctt ctcctgcagc cacatagtgt ttgcgtttgc cctttttggg
     3841 ggtcttcgcg tcaccgcaac gttcttatca tctcccgaag atacgtctga aatctcctgg
     3901 atcgcaacac ggcctgggcg gtaaatacag agaccaggaa agcgagatac acgtaaccga
     3961 acgcggcctg gaccatggaa gcgatctttg cggatgcagt tgcgggaatg aggtcaccgt
     4021 atcccagggt cgtctgcgta ccgatactga aatacaggca atccagaaaa ccaacagctt
     4081 gagtcgccga tttcccttgt cccgtgtggc tcagggaggg caaaccttga acgcccggta
     4141 ggatggcaag gtagaaaaaa gagaaggcaa atgccaccac gatgaagcaa agtactgcgt
     4201 aataaggtct ccgggggctg tctccgacca gaaagtgaac catgttcatc atcgaacctc
     4261 cttttgctgt acttccgtac aggctaaagt gttggatatc tcacgccacc ctcgccctgt
     4321 aattcatctc gatgtaagcg atggtcttat tcccaccatc ctccctttcc acatgaacag
     4381 aagcgggctc gccataaacg atgtgttacg cac
//
LOCUS       DAOQTR010000122         4387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22278_length_4387_cov_3.656741, whole genome shotgun sequence.
ACCESSION   DAOQTR010000122 DAOQTR010000000
VERSION     DAOQTR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22278_length_4387_cov_3.656741"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..966)
                     /gene="phoU"
                     /locus_tag="PKN85_04100"
     CDS             complement(283..966)
                     /gene="phoU"
                     /locus_tag="PKN85_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNT43618.1"
                     /translation="MLEVRIKELKKELVEYATLVEKMIAQSMAGLLERREVPLRRVID
                     ELEPATNDFEIELDELCTGAIAQFQPRARDLRVILMVYKMNSDLERMGDHVANIADSG
                     LFLISHVPVKPLVDIPRLAGETTRSLNDSITAFLNEDASLAQQVCERDHIVDDLQNQI
                     FRELVTFMSSDPATIERSLHRIRVANNLERIADLSTNICEDVIFMVEGRVIKHHREER
                     DEKQKGEKV"
     gene            complement(980..1738)
                     /gene="pstB"
                     /locus_tag="PKN85_04105"
     CDS             complement(980..1738)
                     /gene="pstB"
                     /locus_tag="PKN85_04105"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNT43619.1"
                     /translation="MEDTVLKVEKFSAFFGDNRVLKDVDLTVRRNVVAALMGPSGCGK
                     TTLIRCVNRMHELIPGAGVAGKMTLVDEDIYTMQPIILRRKVGMVFQKPNPFPTMSIY
                     ENVIAGYKLNGIRMAKSELDTIVERSLKRSALWDEVKDALHRKGTFLSGGQQQRLCIA
                     RALAMNPEILLLDEPTSALDPKSTSQIEELFVELKRNVTMLLVTHNIAQAARVSDVTA
                     FIYLGELVEFGPTEKMFTVPKDKRTEEYLTGKFG"
     gene            complement(1720..2568)
                     /gene="pstA"
                     /locus_tag="PKN85_04110"
     CDS             complement(1720..2568)
                     /gene="pstA"
                     /locus_tag="PKN85_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNT43620.1"
                     /translation="MTGGHVGRRLAKDTLFKGAVCLLAFLSLLPLFLILYYITMKGIS
                     VINWDFLFSLPRPIGESGGGVANAVVGTLMLIALSSVISVPCGIAAGIYLSENKTGKL
                     SNTIRLSVIVLQGTPSIVIGIIAYLWVVAPLKTFSALSGGVALSIMMLPVIIKTTEET
                     LKLIPYHLKEASLALGVPYYRTILKVVLPSGISGILTGILLSVARITGETAPLLFTAF
                     GNQFMNVNIFKPIDSLPYRIFYYAMSPYPEWHSFAWGASFILVVLTLALNLIARGISA
                     KWKIQF"
     gene            complement(2565..3482)
                     /gene="pstC"
                     /locus_tag="PKN85_04115"
     CDS             complement(2565..3482)
                     /gene="pstC"
                     /locus_tag="PKN85_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNT43621.1"
                     /translation="MDTGKQRAPSGTDRSEKRFSVCLAGSGLIIVLLLLAIFATLLVT
                     ALPAIKEFGIFFFIGRVWDATTETFGALPFLVGTLLTSFLALVISIPFSIAISIFLGE
                     YFKTGAISTFLRSAVEVLAGIPSVIYGLWGLFFLMPAVRFIEMKLGVAPHGVGILTSS
                     LILAIMIIPFSASIGREVITLVPGDLKEAAYSLGATRFEVIRNIVIPYARSGIIAGIL
                     LALGRAIGETMAVTMLIGNSNFLPTSIFSPANTMASVIANEFAEATGVTAASLIYVAL
                     VLFLVTMVVNIIGTYVIQRISIEASREVS"
     gene            complement(3554..>4387)
                     /gene="pstS"
                     /locus_tag="PKN85_04120"
     CDS             complement(3554..>4387)
                     /gene="pstS"
                     /locus_tag="PKN85_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNT43622.1"
                     /translation="GAPADCSSFAFMTAKELQSAGAPVLHIPTCLGAVVVTYNLPGNS
                     KLNFTPDAVAEIFLGKITKWNDPKIASANPGVKLPDLPVSAVHRADGSGTTYIFSEYL
                     TKVNKEWKEKVGTGKSLKWPAGQIGQKGNPGVAGYVKQTPGAVGYVELLYATQNKMPY
                     GNIKNRAGKFIEPTLKSVSAAANVKIPDDTNVSLTDTEAAAGYPISGFTWLIFYKEQN
                     YGGKAKDRAEALAKLLLWMVGDGQKYVEPLQYAPLSKEAAAKANKLVRSMTYNGAPLV
                     K"
BASE COUNT          843 a         1265 c         1332 g          947 t
ORIGIN      
        1 gacctttgcg gcaaaccttc tttgggaggc aaacccgtcg atcaggctgg gcctccagtg
       61 gatgaggatg ttcacccatt ataacggcgt gggccagggt accggcgcgg gcacgggttt
      121 agccgcctcc agcggcgtca tggaccagta ccggttcgcg gcctggtatt tcttctaaga
      181 atccccgttc ggatatgaaa aggggccttt ttcaaggccc cttttcttta ttggttcccc
      241 gggaggcaat ccctgaagag ctttgccgtc acgcctgcgg gtctagacct tttcaccttt
      301 ctgcttctcg tccctttcct ctcgatggtg cttgatgacc cttccctcga ccatgaatat
      361 cacgtcctcg cagatgttcg tggaaagatc ggcgatgcgc tcgaggttgt tcgcgaccct
      421 gatgcggtgc agcgacctct cgatcgtcgc cgggtccgag gacatgaagg tcacgagctc
      481 ccgaaagatc tggttctgca ggtcgtcgac gatatggtcc cgctcgcaga cctgctgggc
      541 gagggacgca tcctcgttca ggaatgcggt gatgctgtcg ttgaggctcc ttgtcgtttc
      601 gcccgcgagc cggggaatat cgacaagagg cttcaccggc acgtgggata tgaggaacaa
      661 tccgctgtcg gcgatgttcg ccacgtgatc gcccatgcgc tccaggtcgc tgttcatctt
      721 atagaccatg aggatgaccc ttaagtcccg ggccctcggc tggaactgcg ctatggcccc
      781 cgtgcacagc tcgtcgagtt cgatctcgaa atcgttcgtc gccggctcca gttcgtcaat
      841 gacccggcga agagggacct cccgacgctc caggaggcct gccatgctct gggcgatcat
      901 cttctccacc agggtggcgt attcgacgag ctctttcttg agctccttta tcctgacctc
      961 cagcatccct tttagcctcc tatccgaatt ttcccgtcag gtattcttcc gtccgcttgt
     1021 ccttcggcac ggtgaacatc ttctccgtcg gcccgaactc gacgagttcc ccgaggtaga
     1081 tgaaggccgt gacatcggag accctcgcgg cctgggctat gttgtgggtg acgagaagca
     1141 tggtgacgtt cctcttgagt tccacgaaga gctcctcgat ctgcgacgtc gatttcggat
     1201 cgagggccga cgtcggttcg tcaaggagca gtatctcggg gttcatggca agcgcccggg
     1261 ctatgcagag cctttgctgc tgtcccccgg agagaaaggt ccccttccgg tgaagggcat
     1321 ccttgacctc gtcccagagg gccgatctct tcagcgaccg ttcgacgata gtgtccagtt
     1381 ccgatttcgc catcctgatg ccgttgagct tgtatcccgc gatgacattc tcgtatatgc
     1441 tcatcgtggg aaaggggttc ggtttttgaa agaccattcc cactttccgt ctcaggataa
     1501 tgggctgcat cgtgtagatg tcctcgtcga caagcgtcat cttccccgcc acgccggcgc
     1561 cggggatgag ttcgtgcatc ctgttgacgc agcgtatgag ggtcgtctta ccgcaccccg
     1621 acggtcccat gagggcggcg acgacgttcc tcctgacggt gaggtcaacg tccttgagca
     1681 ccctgttgtc gccgaagaag gcgctgaact tctcgacctt tagaactgta tcttccattt
     1741 agcggagatc ccccttgcaa taaggttcag ggcaagcgtc agaacgacaa gtatgaacga
     1801 ggccccccac gcgaaggaat gccactcggg gtaggggctc atggcgtaat agaatatgcg
     1861 gtagggcagg gaatctatcg gcttgaagat attgacgttc atgaactgat tgccgaatgc
     1921 cgtgaagaga agcggtgccg tttcccctgt gatcctcgcc acgctgagga ggatgcccgt
     1981 gaggatgccg cttatgcccg aggggagtac gaccttgaga atggtccggt aatagggcac
     2041 gccgagggcc agcgaggcct ccttcaggtg atagggtatg agcttcaggg tctcctccgt
     2101 ggtcttgatg atcacgggaa gcatcatgat ggaaagggcg acaccgccgg agagagcgga
     2161 aaaggtcttc aggggagcca ctacccagag ataggcgatg atgccgatca ctatcgaggg
     2221 cgttccctgg aggacaatga cactaaggcg tatggtattg ctcagttttc cggtcttgtt
     2281 ctcggaaagg tagatgccgg ccgctatccc gcagggcacg gatatcaccg aggacagggc
     2341 gatgagcatc agcgtcccca cgacggcgtt ggcgacgcct cccccggact cgccgatggg
     2401 gcgcggcagg ctgaagagaa aatcccagtt gatcaccgat attcccttca tcgttatgta
     2461 atagaggatg agaaagaggg ggagaaggga caggaaggca agaaggcaga ccgctccctt
     2521 gaagagcgtg tccttcgcaa ggcgcctgcc gacatgaccg cccgtcatga gacctccctg
     2581 gatgcttcaa tgctgatcct ctgaatgacg tatgtcccga tgatgttgac gaccatggtc
     2641 accagaaaga ggacgagggc gacatagatg agcgacgccg cggtgacgcc cgtcgcctct
     2701 gcgaactcgt tggcgatgac gcttgccatc gtgttggcgg gactgaatat gctcgtcggc
     2761 aggaagttgc tgttgccgat gagcatcgtc accgccatgg tctcgcctat cgcccgtccc
     2821 agcgcgagga gtatgcccgc gatgatgccg gaccgcgcgt aggggataac gatgttccgt
     2881 atcacctcga accgcgtcgc tcccagcgag taagccgcct ccttcaggtc gcccggcacc
     2941 agcgtgatca cctccctgcc gatggacgcc gaaaagggga tgatcatgat ggcaaggatg
     3001 agcgacgagg taagtatccc cacgccatgg ggggcgacgc ccagtttcat ctctatgaac
     3061 cgcacagcgg gcatgagaaa gaagaggccc cagagaccgt agatgacgga agggatccct
     3121 gccaggacct cgacggcgct gcgcaggaat gtcgagatgg cccccgtctt gaagtactca
     3181 ccgaggaaga tggaaatggc tatggagaac gggatggaga tgaccagggc aagaaatgac
     3241 gtgagaaggg tccccacaag gaagggaagg gccccgaagg tctccgtcgt cgcgtcccac
     3301 accctgccga tgaagaagaa gatcccgaat tccttgatcg cgggcagggc ggtcacgagg
     3361 agggttgcga agatcgcgag gagaaggagg acgatgataa ggccgctgcc cgcgagacaa
     3421 acgctgaacc tcttttccga acggtctgtg cctgacggcg ctctttgctt tcctgtatcc
     3481 atacgggttt ggatctatgt ccgggcggcg cctgtaaaca ccgcgccgcc cggtccttcg
     3541 tctcatgatc gccctatttc acgaggggcg cgccattgta tgtcatggag cggaccagtt
     3601 tgttcgcctt cgccgccgcc tctttcgaca acggggcgta ctggaggggt tcgacgtatt
     3661 tctgtccgtc gcccaccatc cacaggagga gtttcgccag cgcctccgcc cgatccttcg
     3721 cctttcctcc gtagttctgc tccttgtaga agatgagcca tgtgaaaccg ctgatggggt
     3781 aaccggcggc cgcctccgtg tctgtcaggg acacattcgt atcgtcgggg atcttcacgt
     3841 tggccgcggc actcaccgat ttgagggtcg gctcgatgaa cttgccggcc ctgttcttta
     3901 tgttgccata gggcatcttg ttctgggtcg cgtagagaag ctcgacgtat cccaccgcgc
     3961 cgggggtctg cttcacgtat ccggcgacgc cggggttccc cttctggccg atctgacccg
     4021 caggccactt gagggatttg ccggtgccga ccttctcctt ccattccttg ttcaccttcg
     4081 tgagatactc gctgaagatg taagtggtcc cgctgccatc ggcgcgatgg acggcactga
     4141 cgggcaagtc gggaagcttc actccagggt tcgcggaggc tattttcggg tcgttccact
     4201 tcgtgatctt gccgaggaat atctcggcca ccgcatcggg ggtgaaattg agtttcgagt
     4261 tccccggcag gttataggtc acgaccacgg ccccaaggca ggtgggaatg tgcaggaccg
     4321 gggcgccggc cgactgcagc tccttcgccg tcatgaaggc gaaggagctg cagtcggccg
     4381 gcgcccc
//
LOCUS       DAOQTR010000123         4387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22280_length_4387_cov_3.425208, whole genome shotgun sequence.
ACCESSION   DAOQTR010000123 DAOQTR010000000
VERSION     DAOQTR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22280_length_4387_cov_3.425208"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKN85_04125"
     CDS             <1..871
                     /locus_tag="PKN85_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT43623.1"
                     /translation="RMNGERDTAGFARYDDIEVERLYMRDLRKLPVISVEEEKMYARM
                     VAEGSPEARKKMIEANLRLVVKIARRYVNQGMSLLDLIEEGNIGLIKAVEKFDLAKEC
                     RFSTYATWWIKQSIERSIANHSRTIRLPVHVSSRVNRISKIISTTVEKTGREPSLEEI
                     SSESGYRIDFVRNLFTMAIKTYSLESYIDENSKLTLEEVIPNPDNEEPLSILEHTRRM
                     EEVASWLDTLASDERKVIMLRFGLDGDEPQTLESIGKTFGVTRERIRQIEQKALNKLR
                     KIVKRRNIGTESI"
     gene            852..2378
                     /gene="murJ"
                     /locus_tag="PKN85_04130"
     CDS             852..2378
                     /gene="murJ"
                     /locus_tag="PKN85_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNT43624.1"
                     /translation="MEQKASESSRRNPALDIIRKGSVITIITLVSRPLGYVREAIQAY
                     LFGATLLVDAFIVAFNFPELIQTLFFSGATSAFLIPVCTKYLHDREEYSRIYSTFINI
                     SVVLTLFVSLLFFFFSGTVVRMIAPGFTEEARAITNNLFLIMIPVIAFHTVLSVMKAF
                     LNAKEHFAAPELSGILWNLFFIASAVLLAKKLGIYSLAIGVSVGSLLQIVMQYPYLRK
                     FGIRYHFSLDLGHPAVATAKRLFTGALIATSIVPINSFVGRIIASYLPHGEVASLSYA
                     FRIFILPFSLFAVPVYTVMFSKLSKLFHDNDMGDFRAHIDSSVVLICVTLIPATILLC
                     ATGDLCVKLLYERGAFTATDTAMTNRALFGYSVGLVFYALSISFVRIFNAMHDMKTPA
                     LIGITSIALNAIIASVLMVPYRNLGISLATSIVSLYNFLFLYVILRHRTGYRLARPAV
                     RDIIKSLLAGILLILLIVLTRRLLPGSLLPPLFASALLTVAVYGIVFKNYYRRLLRRR
                     "
     gene            2383..3234
                     /locus_tag="PKN85_04135"
     CDS             2383..3234
                     /locus_tag="PKN85_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43625.1"
                     /translation="MPVLSRNELTPENVLKHIGKIHDENYGNHGIPLISRVFKDVVDL
                     FNGEWPGYHRCDTQYHDLYHTMQTVPPFVEIISGWNKSGASPRISESFFSYGTIAVLL
                     HDTGYIKELGDDEGTGAKYTLTHVQRSINFANVYLRQVGLPVTEIRHILNIIRCTGVT
                     IDLNVRFRNEQERVVGCALGTADLLGQMSSSDYIDTLPILFDEFAESYGFEGVRKLHK
                     KGSTLYADADELIRSTPTFYEKVALKRFEMMGSMEKYIPRHYGRKRNPYMAAVEKNIR
                     TIRKKWK"
     gene            3493..>4387
                     /locus_tag="PKN85_04140"
     CDS             3493..>4387
                     /locus_tag="PKN85_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43626.1"
                     /translation="MTKTIREFHVSRKARDTYHFSDALFALSGNVIFTNFLAARTFAQ
                     KMNDKRDLILYPESAIRAGALIAMGLIDEILHYVVSVYAESVRRDVMAGALSHLEATL
                     GPAELERTIRQFSDEFPPLAVYQRKTTLDEYLSGQTGETPNRWIVLEEMLLLWLANMN
                     PAFSPFTELFDDSSLRRETSFLKTIAELRVFFDSQPPFGPEGQNLIDLLRSPAVAVPH
                     SLSGQLEYIMERWGYLLGSRFISRMLKSLDLFKEEEKMPFLGPGKAQVYRYTGLEFEP
                     EQFSADKDWMPRLILIAKNVYV"
BASE COUNT          972 a         1391 c         1094 g          930 t
ORIGIN      
        1 gagaatgaac ggggaaaggg acacggcggg ctttgcccgc tacgacgata tcgaggtgga
       61 gcgcctgtac atgagggatt tgcgcaagct gcccgtcatc tccgtcgaag aagagaagat
      121 gtacgcccgc atggtggctg aagggagccc cgaagcccgc aagaagatga tagaggccaa
      181 cctgcgtctc gtggtgaaga tcgcccgcag gtacgtcaac cagggaatga gccttctcga
      241 cctcatcgag gagggcaaca taggcctcat caaggcggtg gaaaaattcg acctggcgaa
      301 ggagtgccgc ttctccacgt atgccacgtg gtggataaag cagtccatcg agcggtcgat
      361 agcgaaccac tcccggacca tacggctgcc cgtccacgtc tcatcccggg tgaacaggat
      421 atcgaagatc atcagcacca ccgttgaaaa gacgggccgg gagccttcgc tggaagagat
      481 atccagcgaa tcgggatacc gcatcgactt cgtcaggaac ctcttcacca tggcgataaa
      541 gacctactct ctcgagagct atatcgacga gaactcgaag ctcaccctcg aggaggtcat
      601 tcccaacccc gacaacgaag agccgctttc catcctggag cacacaaggc gcatggagga
      661 ggtggcctcg tggcttgaca ccctggcctc cgatgagaga aaggtcataa tgctgcgctt
      721 cggcctcgac ggggacgaac cgcagacact ggaatccatc ggcaaaacct tcggcgtgac
      781 gcgggagcgc atacgccaga tagaacagaa ggccctcaac aagctccgca agatcgtcaa
      841 gagacggaat attggaacag aaagcatctg agtcctcccg ccggaatccg gcactggaca
      901 tcatccgcaa aggctccgtc atcaccatca taaccctcgt ttcgagaccg ctgggttacg
      961 tgcgcgaagc gatacaggca tacctcttcg gggccactct gctcgtcgac gccttcatcg
     1021 tggccttcaa ttttcccgag ctcatccaga cgctcttctt ttccggggca acgagcgcct
     1081 ttctcatccc cgtgtgcacg aagtacctcc acgaccgcga ggaatattcc cgcatatact
     1141 cgacgttcat aaacatctcc gttgtcctca cgctcttcgt ctccctgctg ttcttttttt
     1201 tcagcggcac cgtcgtgcgg atgatcgcac ccggcttcac cgaagaggcg agggcgatca
     1261 cgaacaacct cttcctcatc atgatacccg tcatagcctt ccacacggtc ctttccgtca
     1321 tgaaggcatt cctgaacgcg aaggaacact tcgctgctcc ggaattgtcg gggatactgt
     1381 ggaacctctt cttcatcgcc tcggcagtcc tcctggcaaa aaagctcggc atctacagcc
     1441 ttgccatagg cgtcagcgtg gggtccctgc tgcagatcgt catgcagtat ccttatctca
     1501 ggaaattcgg catccgatac cacttctccc tcgatctcgg ccaccccgcc gtcgccaccg
     1561 ccaaacgtct tttcaccggc gcgctcatcg cgacgtccat cgtgcccatc aacagcttcg
     1621 tcgggaggat catagcctcc tacctccccc acggggaggt ggcgtccctc tcctacgcct
     1681 tcaggatatt catccttccc ttcagcctct tcgcggttcc cgtgtacacg gtgatgttct
     1741 caaagctgtc gaagctcttc cacgacaacg acatgggcga tttcagggcc cacatagaca
     1801 gctccgtcgt tctcatctgc gtgaccctca tcccggcgac catattgctg tgcgccacgg
     1861 gcgacctgtg cgtgaagctc ctctacgaac gcggagcatt taccgcaaca gacacggcaa
     1921 tgacgaaccg ggccctcttc ggctacagcg tggggcttgt cttctacgcc ctttccatct
     1981 ccttcgtgcg aatattcaac gccatgcacg atatgaaaac gccggccctt atcggcatca
     2041 cctccatcgc gctcaacgcc atcatcgcct ccgtcctcat ggtcccttac aggaacctcg
     2101 ggatctccct ggccacgtcc atcgtgtccc tctacaactt cctcttcctc tatgtcatct
     2161 tgaggcacag gacggggtac aggctggccc gccccgccgt gcgggacatc atcaagtccc
     2221 ttcttgccgg tatactcctc atccttctca tcgtcctcac acgccggctc ctgccgggaa
     2281 gccttcttcc cccgcttttt gcaagcgctt tgttgaccgt cgccgtttat ggtatagtgt
     2341 ttaaaaatta ttaccgaaga ctcttgcgca ggagatgatg ccatgcccgt attgtcaagg
     2401 aacgagctga cgcctgagaa cgtcctgaag cacatcggga agatccatga cgagaattac
     2461 ggcaatcatg gcatacctct gatctcgcgg gtcttcaagg acgttgtcga cctcttcaac
     2521 ggcgaatggc ccggctatca ccggtgcgac acgcaatacc atgacctcta ccacaccatg
     2581 cagaccgtgc cgcctttcgt ggagatcatc agcggctgga acaagagcgg agcctccccg
     2641 aggatctcgg agagtttctt ttcttacggt accatcgcgg tccttcttca cgatacgggg
     2701 tacatcaagg agcttggcga cgatgaaggc acgggtgcca aatataccct cacccatgtc
     2761 cagcgcagca tcaatttcgc caacgtctac ctccgtcagg taggcctgcc cgtcaccgaa
     2821 atacgccaca tcctcaacat catcaggtgc accggcgtca ccatcgacct caatgtccgc
     2881 ttccgcaacg agcaggaacg cgtggtcggc tgcgccctgg gcactgccga tctgttgggt
     2941 cagatgtcat cttccgatta catcgacacc ctccctatcc tcttcgacga attcgccgaa
     3001 tcctacggtt tcgaaggggt caggaagctt cacaagaagg gctccaccct gtacgccgat
     3061 gccgacgaac tcatcaggtc gacgcccaca ttctatgaaa aagtggccct caagcgtttt
     3121 gaaatgatgg gttccatgga aaagtacatc ccccgccact atggccgaaa aaggaaccca
     3181 tacatggccg ctgtggaaaa gaacatccga accatcagga agaaatggaa ataaacagag
     3241 ttccgggtcg cgggttgcgt gttccgggtt gaggcaaagc ctcaggaagt gaaaacgctt
     3301 tcaggtctca ccgaaaactt cccctctcaa ggttccaatc ttcccggaca atcggtccct
     3361 ccttggtttt tgacccgaaa cgcgcaaccc ggaacccgca actgtctttt gcctgaaacg
     3421 tgaaacctca attgacggaa tccttttact gtgttagcat tccttctacg taagggcaaa
     3481 tggtgtagac ctatgacaaa gaccatcaga gagttccacg tatcacggaa ggccagggac
     3541 acctatcatt tcagcgacgc cctcttcgcc ttgagcggca acgttatatt taccaacttt
     3601 ctcgcggccc gcaccttcgc gcagaagatg aacgacaaac gggaccttat cctctacccc
     3661 gagagcgcga tacgcgcggg tgcgctcatc gccatgggcc tcatcgacga gatccttcat
     3721 tacgtggtca gcgtgtacgc ggaatccgtt cggagggacg tcatggccgg tgccctctcc
     3781 caccttgagg ccactcttgg ccccgcggaa ctggaaagga cgatacgcca gttcagtgac
     3841 gaatttccgc cccttgccgt ctaccagagg aagacgacac tcgatgagta cctcagcggc
     3901 cagacgggtg aaacccccaa ccggtggata gtcctcgaag agatgctcct tctctggctc
     3961 gcgaacatga accccgcctt ctcccccttc accgaacttt tcgacgactc ttccttgagg
     4021 agagagacct cctttctcaa gaccatcgcc gagttgaggg tctttttcga ctcccagccc
     4081 cccttcggcc cggagggcca gaacctgata gacctgctcc gcagcccggc cgtcgccgta
     4141 ccccattcgt tgtcggggca gctggaatac atcatggaac ggtggggata ccttctcgga
     4201 tccaggttca tttcccgcat gctcaagagc ctcgaccttt tcaaggaaga ggagaagatg
     4261 ccttttctcg gacccggcaa ggcccaggtg tatcgctata ccggtctcga gttcgaaccg
     4321 gaacagttca gcgccgacaa agactggatg ccgcgtctta tcctcattgc caagaacgtc
     4381 tacgtgt
//
LOCUS       DAOQTR010000124         4373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22375_length_4373_cov_3.857341, whole genome shotgun sequence.
ACCESSION   DAOQTR010000124 DAOQTR010000000
VERSION     DAOQTR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22375_length_4373_cov_3.857341"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..637)
                     /locus_tag="PKN85_04145"
     CDS             complement(53..637)
                     /locus_tag="PKN85_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNT43627.1"
                     /translation="MANDTAINEALQTLLDGNKRFAAMEQTHPNQDRTRRDEVKAGQK
                     PFAVIVGCSDSRIPPEILFDQGIGDLFIIRLAGNIVDDTALGSIEYAVDHLGTRLVVV
                     LGHSKCGAVTATAKGGEAHGHIGSIAQAILPALENVKGQSGDLVDNAIRENAKLVTAT
                     IASSKPILEKMVEEGKIGVVPLYYDIDTGLVERL"
     gene            874..2862
                     /gene="acs"
                     /locus_tag="PKN85_04150"
     CDS             874..2862
                     /gene="acs"
                     /locus_tag="PKN85_04150"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNT43628.1"
                     /translation="MADATEETRLYPPLKEFVDQAYVKSRDEYEKMWKQSIQDPETFW
                     GGIAKELSWFKPWLKVNREDFAKGEVEWFIGGKTNIAYNCLDAQVEKGKGDKVAILFQ
                     GEPEDDVVKLTYKEMLSMVSKFANVLKKKGVRKGDRIAIYLPMIWQLPVVMLACARIG
                     AVHTIVFGGFSAEALRDRILDAGAKMLITTNGYWRSGKTVSSKANADTACDLCLAQGH
                     TVDKVVVVKRLDNFDVPMKAGRDTWFEEELAAADITDDCPAEMMDAEDPLFILYTSGS
                     TGKPKGVLHTVGGYMVYTYSTFKYIFDYKDKDVFFCTADIGWVTGHSYIVYGPMTNGA
                     TSIVFESVPTFPNPDRFWQIVEKFKVSIFYTAPTAIRALMKEGEKWPQGRNLSSLRLL
                     GSVGEPINPEAWLWYHKYIGGEKCPIADTWWQTETGGILITSLPGAWPGKPGYATLPF
                     FGVEARALQSRPDASKPAEDAPMNEQGELCIGRSWPGMMRGVFGEPERFFNTYFVQQP
                     GFYFSGDGALRDENGYFRLMGRIDDVVNVSGHRMGTAEVEAALNSFNTAVAESAVVGF
                     PHEVKGEDLYAYIILKTGVEGTDALKKELVAHVRKEIGPIASPGKIQFVPGLPKTRSG
                     KIMRRILRKVASGEIDQLGDTTTLADPSVVDDIVKGRQ"
     gene            2940..3914
                     /locus_tag="PKN85_04155"
     CDS             2940..3914
                     /locus_tag="PKN85_04155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT43629.1"
                     /translation="MDERDPSTSGGTCRLSTTEAYHRQEIDGLGWELTVCNSLHPAGT
                     PMRRVLEKDASYGHLLYDHLSRFMPLMDMTSILEIGAGYGYLMKDFLERNPGLRPCLM
                     DISPALLGKQRETLAGHDVSYVLQDALEADAATLQRFELVILNENLGDFPTAVDVDHR
                     VFEDREGSGDLPWRICRLFDTYNLEISQTPFNVNLGALEMVEKLCSSEVPRIFIGEHS
                     CEAALPPALKPYIPLAFDGNPRRIRLKGHDEYTIKFSHLRRIAEYYGYRTVRGPFADY
                     IVPNITEELKAIFATRGLYCDSEEMIYHFIGDLYEYEYLVMMKEGDRR"
     gene            complement(4057..>4373)
                     /locus_tag="PKN85_04160"
     CDS             complement(4057..>4373)
                     /locus_tag="PKN85_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43630.1"
                     /translation="MMLSAGKGSVGLFAVAFCGFWLCLGGWLAIGPASTASFFGLEGY
                     AQKYGIVFSAYGLGAIVGGIISGMAKDTFGSYTAAFWPTLILAIVGIFVAFFVLRPPK
                     AR"
BASE COUNT          968 a         1258 c         1273 g          874 t
ORIGIN      
        1 agaagcctga tcgtcttgtt tctaacctga aacctgaaac ctgagactgc ttctagagcc
       61 tttccaccag gcccgtgtcg atgtcgtagt agagcgggac gacccctatc tttccctcct
      121 ctaccatctt ctccagtatc ggtttcgatg aggctatcgt tgccgtcacg agcttcgcgt
      181 tctccctgat ggcgttgtcc acgaggtctc cggattgtcc cttgacgttc tcgagggcgg
      241 ggaggatcgc ctgtgcgatg ctgccgatgt ggccgtgggc ctcgccgcct ttcgccgtgg
      301 cggtgactgc accgcatttg gagtggccga ggacgacgac gaggcgggtg ccgaggtggt
      361 cgacggcgta ctcgatgctg ccgagagctg tgtcgtcgac gatgttcccg gcgagcctga
      421 tgatgaagag gtcgccgatc ccctggtcga agaggatctc gggagggatg cgtgagtcgg
      481 agcatcccac tatgacggcg aagggtttct gacctgcctt gacctcatcg cggcgggtcc
      541 ggtcctggtt gggatgcgtc tgttccatgg cggcgaaacg cttgttgccg tcaagcagtg
      601 tctgcagcgc ctcgttgatg gcggtatcgt tggccataat agttccccct ggttttttag
      661 agagtatcat aatggattta ggcctcttca tcaagtcaaa agcccgcgtt cgcttcgaat
      721 gcccggaatg ccacaagtat tgtgaaaaga ttaacaaaat tttattgaca tcaccacccg
      781 gcgcgtttaa aatagtctgt tcaataagct aaatacgggt agcttaagct gaatacgggc
      841 agcttcattt aaaaatcaat aaggaggtta tgtatggcag acgcaacaga agaaaccaga
      901 ttgtatccac cccttaagga attcgtcgac caggcctacg tgaagagccg cgatgaatac
      961 gaaaagatgt ggaaacagtc cattcaggac cccgaaacat tctggggcgg catcgcgaaa
     1021 gagcttagct ggttcaagcc gtggctgaag gtcaacaggg aagatttcgc gaagggcgaa
     1081 gtggagtggt tcatcggcgg caagaccaac atcgcctaca actgcctcga tgcccaggtc
     1141 gagaagggca agggagacaa ggtcgccatc ctcttccagg gcgagccgga agacgatgtc
     1201 gtgaaactga cctacaagga aatgctttcc atggtttcca agttcgccaa cgtgcttaaa
     1261 aagaaaggcg tccgcaaggg cgacaggatt gccatctacc tgcccatgat ctggcagctt
     1321 cctgttgtca tgctggcctg cgcaagaata ggcgccgtcc atacgatcgt tttcggcggg
     1381 ttctccgctg aggcgctccg cgacaggatc ctcgacgccg gcgcaaagat gctcatcacg
     1441 acgaacggct actggcgttc gggcaagacg gtgagctcca aggcgaacgc cgacacagcc
     1501 tgtgatctgt gcctcgcgca gggccacacc gtggacaagg tcgtcgtcgt caagaggctt
     1561 gacaacttcg acgtcccgat gaaagccggt cgcgacacct ggttcgagga agaactggcg
     1621 gctgcggaca tcaccgacga ctgccccgcc gagatgatgg acgcggaaga tccgctcttc
     1681 atcctctaca catcaggttc aacgggtaag ccgaagggtg ttcttcacac cgtcggcggc
     1741 tacatggtct atacgtattc aaccttcaaa tacatcttcg actacaaaga caaagacgtg
     1801 ttcttctgca ccgccgatat cggctgggta acgggccaca gctacatcgt ctacggcccg
     1861 atgacgaacg gagcgacctc gatcgttttc gagtccgtcc cgacattccc gaaccccgac
     1921 aggttctggc agatcgtcga gaagttcaag gtcagcatct tctacaccgc ccccacagcc
     1981 atcagggccc tcatgaaaga aggcgaaaag tggccgcagg gcaggaacct ctcctcactg
     2041 agactccttg gttccgtcgg cgagccgatc aacccggaag catggctctg gtaccacaag
     2101 tatatcggcg gagagaagtg ccccatcgcc gacacctggt ggcagacgga gacaggcggc
     2161 atcctcatca cctctcttcc tggcgcatgg ccgggaaaac cgggctacgc gacgcttcct
     2221 ttcttcggcg ttgaggcccg cgcactccag tcacgccccg acgcgagcaa acccgcggaa
     2281 gacgcgccca tgaacgagca gggcgagctc tgcatcggca ggtcatggcc tggcatgatg
     2341 agaggcgttt tcggcgaacc cgaaagattc ttcaacacct actttgtcca gcagccgggg
     2401 ttctacttct ccggtgacgg tgcgttgagg gacgagaacg gctacttcag actgatgggc
     2461 cgtatcgacg acgtcgtcaa cgtatccggc cacaggatgg gaaccgcaga ggtcgaggca
     2521 gccctcaact ccttcaacac ggcagtcgcg gaatccgccg ttgtcggctt cccgcacgaa
     2581 gtgaagggcg aggaccttta cgcatacatc atcctgaaaa caggcgttga gggaacggat
     2641 gctctcaaga aagagctcgt tgcccacgtc aggaaagaga tcggccccat cgcatcaccg
     2701 ggcaagatcc agttcgtacc cggtctgccg aagactcgct ccggcaagat catgagaagg
     2761 atcctgagaa aggtcgcctc cggcgagatc gatcagctcg gcgataccac cacgctggca
     2821 gatccgtcag ttgtcgacga tatcgtaaag ggcaggcagt aagaccggcc cgccttcttg
     2881 aaaggcaatg cgaggccccc gggatcatac ccgggggctt ctttttgtga ggacagtacg
     2941 tggatgagag agacccatca acctccggcg gcacctgccg gctgagcacg acggaagcgt
     3001 atcacaggca ggagatcgac ggtctggggt gggagctgac cgtctgcaat tccctccatc
     3061 ccgccggtac gccgatgcgc cgtgtcctcg agaaggatgc ttcctacggg catctgctct
     3121 atgaccatct cagccgtttc atgcccctca tggacatgac gtccatcctc gagatcggag
     3181 ccggctacgg ctaccttatg aaggatttcc tggagaggaa ccccggattg cgtccctgtc
     3241 tcatggacat ctcaccggcg ctcctcggga aacagcggga gacactggcc ggtcacgatg
     3301 tatcctacgt gctgcaggat gccctggagg cggacgccgc aacccttcag cgcttcgagc
     3361 tggtgatcct caacgagaac ctcggtgact ttccgacggc ggtggacgtg gaccatcggg
     3421 tcttcgagga ccgggaggga tccggtgacc tgccctggag gatatgccgt ctctttgaca
     3481 cctacaacct cgagatatcg cagactcctt tcaacgtcaa cctcggggct ctggagatgg
     3541 tcgagaagct ctgttcttca gaggtgcccc gcatcttcat aggggaacac agctgtgaag
     3601 cggcccttcc tccggccctc aagccgtaca tacccctcgc gttcgacggc aacccccggc
     3661 gcatccgctt aaagggccac gacgaataca ccatcaagtt ctcccacctg cgccgcatag
     3721 ccgaatatta cggttacagg accgtccgcg gcccctttgc cgactacatc gtgcccaaca
     3781 tcacggagga attgaaggcg atctttgcca cgaggggcct ctactgcgac tccgaagaga
     3841 tgatctacca cttcattggc gacctgtacg agtatgagta cctggtgatg atgaaggaag
     3901 gggacaggcg gtagggatca ggggatggca ggggatggca ggggaaaagg aggggttgag
     3961 ggtgagggga ttataggtcc tatcttttcc tatcccccct tgttcacaaa aaaagaggca
     4021 tgttcccatg cctctttttt tgtgcgctct gtgagcctac ctggccttcg gcggtctgag
     4081 aacgaagaat gccacgaaga tacccacgat ggcgagtatc agggtaggcc agaaggcggc
     4141 cgtgtagctt ccgaaggtgt ccttggccat acctgagatg atgcctccga cgatggcgcc
     4201 gagaccgtag gcgctgaaga cgatgccata tttttgagca tacccctcga gaccgaagaa
     4261 ggatgccgtg cttgccgggc cgatggcaag ccatccgcca aggcagagcc agaagccgca
     4321 gaaggcgacg gcgaaaagac ccaccgatcc ctttccggcg ctgagcatca tga
//
LOCUS       DAOQTR010000125         4370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22389_length_4370_cov_4.183082, whole genome shotgun sequence.
ACCESSION   DAOQTR010000125 DAOQTR010000000
VERSION     DAOQTR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22389_length_4370_cov_4.183082"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKN85_04165"
     CDS             <1..498
                     /locus_tag="PKN85_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43631.1"
                     /translation="REITLVTSQWVNSYKRLVPGYEAPVYISWARRNRSTLVRVPLYK
                     PGKENATRVEYRSPDPACNPYLAFSCMLRAGLEGIRNKYPLPGAVEEDVYDMTPERRK
                     ELDIGSLPGSLYEAIELTEGSKLVRDALGDHVFEKLIENKKIEWDRFRAHVSNYEIET
                     YLPVL"
     gene            577..945
                     /locus_tag="PKN85_04170"
     CDS             577..945
                     /locus_tag="PKN85_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT43632.1"
                     /translation="MIVRNFNDPEVLETRYRAHWGATAWMVMTSRFLEGIEFLAYAIL
                     PPGNTIEEHVDEVEEIYMIFRGGGKMKVGDEIREVKEGDSIWIPAGEPHALENTKEEE
                     TFVLVIAAYPFKRFLQMAEK"
     gene            979..1662
                     /locus_tag="PKN85_04175"
     CDS             979..1662
                     /locus_tag="PKN85_04175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT43633.1"
                     /translation="MNIRPGLNINIIINIDHMRETVDVGNSIIHEMAGDRMIVAQTDP
                     PVSRTHLGKELFVTFLDRSGGEPHRFGFPAIVLEFLKEFELSASLKKVQALVLRKAGD
                     FEEYNLRMFYRLEPPSDCGIRIAVDGKGVNLIDISIGGAKFSHEKVFPFEIGEPVRLS
                     LYVDEVAYPVEAKVLRVWEPENERIRKTVALASVQFTDMEAYLKNVLARKIRDVEREM
                     RYKELHPAR"
     gene            1721..1852
                     /locus_tag="PKN85_04180"
     CDS             1721..1852
                     /locus_tag="PKN85_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43634.1"
                     /translation="MKHQGQCPPSRTVPISLRRTLPVDEERMNAMRETATQTLHATL"
     gene            complement(2355..2849)
                     /locus_tag="PKN85_04185"
     CDS             complement(2355..2849)
                     /locus_tag="PKN85_04185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT43635.1"
                     /translation="MIRICADRDFDSILEIINDGARAYKGIIPHDCWHEPYMARPELS
                     HEIADGVVFYGCEEEGVLVGVMGIQDRGDVSLIRHAYVRTARRNCGIGTRLLGFLQST
                     TTKPLLVGTWAAAAWAIAFYEKRGYKVLPPHDKDRLLRKYWNIPRRQVDTSVVLAGPG
                     WPHP"
     gene            complement(2925..3785)
                     /locus_tag="PKN85_04190"
     CDS             complement(2925..3785)
                     /locus_tag="PKN85_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNT43636.1"
                     /translation="MELQGSSDIVRFQRASDFDGLEFLHATFVQHSFPRHTHDTFAIG
                     VIESGVQATYYKGSTHIATCRDICLINPGEVHTGFSPHPSGWTYRVFYPEPSLLETVA
                     SQLLPREHGLPHFSSPVIKDRLLSTAVLSFLRVLDLSDVTIERESLLYAVIAQMISRH
                     ADRKGPMSPVPRHAERPVVKTLLEYLHHHFTDNVTLRELSDVAALSEFHLLRLFRSAV
                     GLPPHAYIIQKRVDHARRLLLTGLPIAHVGIEAGFADQSHFTRAFKRIVGVTPGCYRS
                     SNSVQDFSDL"
     gene            complement(3960..>4370)
                     /locus_tag="PKN85_04195"
     CDS             complement(3960..>4370)
                     /locus_tag="PKN85_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNT43637.1"
                     /translation="VSIWPMAVVRADEAATVIGKGSAILDLSLLESPAGLPVAIGEGS
                     IISHGAIVHGASIASGVLVGAGAMVLDNAVVGEGSIVAAGAVVTPRTVIPPNSLVLGS
                     PGRVARATTLEERQGVTKQARGLFRKSRAYLAEP"
BASE COUNT          954 a         1182 c         1475 g          759 t
ORIGIN      
        1 cgtgagatca ccctggtgac gagccagtgg gtgaactcgt acaagaggct ggtgcctggc
       61 tacgaagctc cggtatatat ttcctgggcg aggcgcaaca ggtcgacact ggtccgggtg
      121 cccctgtata aaccggggaa ggagaacgcg acgcgggtgg agtacaggag ccccgacccg
      181 gcgtgcaacc cctatctcgc cttttcctgc atgctgaggg cgggcctcga gggcatacgg
      241 aacaagtacc ccctccccgg cgccgttgag gaagatgtct acgatatgac gccggagcgc
      301 agaaaggagc tggatatagg gtcccttccg ggaagcctgt acgaggccat agaattaacg
      361 gaaggatcga agctggtccg ggacgccctt ggcgaccatg tgttcgagaa gctcatcgag
      421 aacaagaaga tagaatggga caggttcagg gcgcacgtgt cgaactacga gatcgagacg
      481 tacctgcccg tcctttagaa tcgattgcag ccgcctgacc cgttcgatgg tcttgagcgg
      541 agaaaacgac acgactgcag gcggggaagg agtcagatga tagtccgtaa tttcaacgat
      601 cctgaggtgt tggagacgag atacagggcg cactgggggg cgaccgcgtg gatggtaatg
      661 acgagccggt tcctggaagg catagagttc ctcgcgtatg ccatactgcc cccgggcaac
      721 accatcgagg agcatgtcga cgaggtcgaa gagatataca tgatctttcg gggcggcggg
      781 aagatgaagg tcggggacga aatccgggag gtgaaagagg gggattcgat ctggattccg
      841 gccggtgagc cgcacgccct tgagaacacg aaggaggagg agaccttcgt cctcgtgata
      901 gcggcatatc cttttaagag attcctgcag atggccgaga aatagacagg aaggacggtt
      961 tcctgtccac acgcgaacat gaacatcaga ccaggcctga acatcaatat tatcatcaat
     1021 atagaccata tgagggagac cgttgacgtc gggaattcca taatccacga gatggcgggc
     1081 gacaggatga tcgtcgccca gacggaccct cccgtatcca ggacccatct gggcaaggag
     1141 ctctttgtga cctttctcga caggtcgggc ggagaacccc accgcttcgg ttttccggcg
     1201 atcgtccttg agttcctgaa ggaattcgag ctttcagcgt ctctcaagaa ggtccaggcg
     1261 ctggtcctga gaaaggcggg ggacttcgag gaatacaacc tgaggatgtt ctacaggctt
     1321 gaaccgccgt cggactgcgg catcaggata gccgtggacg gtaagggtgt gaacctcatc
     1381 gacatatcca tcggaggagc caaattcagc cacgagaagg tctttccctt tgagatcggt
     1441 gaaccggtaa ggctgagcct gtacgtggac gaggtggctt acccggtgga ggcgaaggtc
     1501 ctcagggtgt gggagcccga gaacgagcgg atacggaaga ccgtcgccct ggcgtcggtt
     1561 cagttcacgg acatggaggc ctacctgaag aatgtcctgg cgcgcaagat tcgcgatgta
     1621 gagagggaga tgcgctacaa ggagctccac cccgcaagat gaccgcagtc gcggtaatga
     1681 tgtaagacag cgagatcatt gaacaaaatg gtagttccac gtgaaacatc agggtcaatg
     1741 ccctccttcg cggaccgtcc ccatatcgct gcggcgaacc cttcctgtcg atgaggaaag
     1801 gatgaacgcc atgagggaga cggccacgca gaccctccac gccacgctgt aattccggaa
     1861 aagaccgaaa aggaaaagga agcgggtggc cagaggcccc atgccgtacc cgagggtgct
     1921 ggcgagggta gtaacgccga aagggcgccg tgatgatcca ggccgaaccg ctcagcggga
     1981 agaagcggca ccgcgcaagg gatctcccca taggtgatgc ggaagagtct ggttgagacc
     2041 ggagaacccg gaaatcccgg gatgcaatga taagaccgaa ccgggcggcc atcatgaggg
     2101 tggcggcgat aaggaccggc cttgtgccga gcttgtcggt cagccatttc ggcggtgcca
     2161 ccagtgtgcc gccggggaag aagcagagaa gaatatgcaa ccaagggggg acgctctcgt
     2221 tcagccgggt tccgcgggga ggggtttgag agagacgccg aaggaatgga gcgcgcccta
     2281 catgacgagc atgatgatga acccggccgg aacgagaaaa tggccggaga ataacggggg
     2341 ccgccgggga gagatcacgg gtgtggccag ccggggccgg cgagcacgac ggaagtgtcc
     2401 acctggcggc ggggtatgtt ccagtacttc ctgaggaggc gatccttgtc gtgcgggggg
     2461 agcaccttgt agcctctctt ctcgtagaag gctatcgccc aggcagcagc cgcccatgtg
     2521 ccgacgagaa gcggcttggt ggtggtcgat tgcagaaagc cgaggagcct cgtgccgatg
     2581 ccgcagtttc tccgcgccgt ccggacgtag gcgtggcgga taagagacac gtcgccgcgg
     2641 tcctgtatgc ccatgacgcc gacgagaacg ccctcttcct cgcagccgta gaagaccacg
     2701 ccgtccgcga tctcgtggct gagctccggg cgggccatgt aaggttcgtg ccagcaatcg
     2761 tgcggtatga tgcccttgta ggcccgggcc ccgtcattga tgatctccag gattgagtca
     2821 aagtcgcgat cggcgcagat ccttatcatt ctggttcctc cttggcgcgg ctgcgggagg
     2881 cgccgggtgg cggggactcc cgcagcctgg ttagagtgta aggctcaaag gtcggagaag
     2941 tcttgaacgg aattgctgga gcggtagcag cccggggtga ccccgacgat ccttttgaag
     3001 gcgcgggtga aatggctctg gtcggcaaag cccgcctcga tgccaacgtg ggcgataggc
     3061 aggcccgtca ggaggaggcg ccgggcgtgg tccacccgtt tctggatgat ataggcgtgg
     3121 ggagggagcc ccacggcaga tctgaatagg cggagaaggt ggaactcgct cagagcggcg
     3181 acatcagaaa gctcgcggag ggtgacattg tcggtgaaat gatggtggag gtattcgagg
     3241 agcgtcttga ccacgggcct ttcggcgtgt ctgggaaccg gagacatcgg ccccttcctg
     3301 tcggcgtggc gtgatatcat ctgcgctatc accgcgtaaa ggagagactc gcgctcgatc
     3361 gtgacgtccg agaggtcgag gaccctgaga aaggagagaa cggccgtgga gaggaggcga
     3421 tcctttatga cgggcgagga gaaatgcgga agtccgtgtt cgcgggggag cagctgggag
     3481 gcgactgtct cgaggagtga aggttcgggg tagaagacgc gatacgtcca gcccgacggg
     3541 tggggagaga accccgtgtg gacctcgccc gggttgatga gacagatgtc gcggcaggtg
     3601 gcgatatggg tggaaccctt gtaataggtc gcctggacac cgctttctat gacgcctatg
     3661 gcgaaggtgt cgtgagtatg ccggggaaag gaatgctgga cgaaggtggc gtggaggaac
     3721 tccaggccat cgaaatcgga agcgcgctgg aacctgacaa tatcgctgct accctgcaat
     3781 tccattccac ctaccctgcg gcgctccctg ccagtgtgcc gggaaagaga cgcccccgcc
     3841 tagaagactc ttgccgctga aagagcgcat tctccgtgcg cgaggatggc agcgacacgc
     3901 tctgaccggc aattgtcttg acagtcgctc aaccccgcgg tctttccctt gagcctgcct
     3961 cacggctccg ccagatacgc cctcgacttg cggaaaagcc cccgggcctg cttcgtgacg
     4021 ccctgccttt cttcaagcgt cgtcgcgcgc gcgaccctgc ccggcgaacc gaggacaagc
     4081 gaattggggg gtatgacagt ccggggcgtc acgacggcac ctgcggcaac gatggaccct
     4141 tcaccaacga cggcgttgtc aagaaccatg gcgccggcgc ccacgaggac gccggacgcg
     4201 atgctggccc cgtgcacgat ggccccgtgg ctgatgatgg atccctctcc gatggcgacc
     4261 ggaagccccg ccggggattc caggagggac agatcgagga tggcggatcc cttcccgatg
     4321 accgtcgcgg cctcatcggc cctcacgacc gccatcggcc agatgctgac
//
LOCUS       DAOQTR010000126         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22520_length_4354_cov_3.651779, whole genome shotgun sequence.
ACCESSION   DAOQTR010000126 DAOQTR010000000
VERSION     DAOQTR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22520_length_4354_cov_3.651779"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PKN85_04200"
     CDS             <1..868
                     /locus_tag="PKN85_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNT43638.1"
                     /translation="GRSVIVIDYDPGRTENRYYADIARYLRSMRVPVPEVTGHDPEAH
                     LMVLEDLGSKDLHAFAGEDWPTRRALYQKTLAAAKRLHGIQEKDFPSGTVKLMDGFGP
                     ELYLFEQNYFLEHFVRSHCSVDLEPAFESRLMSELSRLTDELCSLRRCLVHRDLQSQN
                     VMVLGSEPYFIDFQGMRFGNPFYDLGSLLCDPYVTFTPGQREELLSFYYILSSQELDW
                     DAFQASFWSASVERLMQALGAYGFLGRAKGLTAFLGHIPAGLRNLSMAASHVPWLSTL
                     RELTAECSVALG"
     gene            1059..2534
                     /locus_tag="PKN85_04205"
     CDS             1059..2534
                     /locus_tag="PKN85_04205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNT43639.1"
                     /translation="MRPVTAVCSYTAGPMFLPALRNLAASDLIEEVLVVAREPIPDIL
                     PRCTVINTGPFASRETFETILAKTATEYLLFIPRTIPMLAGMATLERFIRAARSTGAG
                     TVYSDFHDDTGKGKTLHPLIDYQPGSVRDDFNFGALALLDASIVRDTLQRYGRTPPLR
                     HGALYDIRLKMSIDHGIHHIAEPLYSVVVHGDTPGADAHFAYVDPRNQALQREMETVF
                     TDHLKRINAYLPPDRLKTMEEPAEPFPVRASVIIPVKNRRKTIADALQSALSQETDFT
                     FNILVVDNHSTDGTTEVIADMAGRNPAIRHIIPRRKDLGIGGCWNAAIMDDACGRYAV
                     QLDSDDLYENHLVLETITDIMRDGRYAMVVGSYTIVNERLEQIPPGLIDHREWTADNG
                     HNNALRVNGLGAPRAFSTTAIRRIGFLNVSYGEDYAAALRMSREYRIGRIYESLYLCR
                     RWQGNTDAGLSLEAANRNDAFKDSLRTAELVERQKLAGESP"
     gene            2535..3512
                     /locus_tag="PKN85_04210"
     CDS             2535..3512
                     /locus_tag="PKN85_04210"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HNT43640.1"
                     /translation="MTRALRDRTYATFEGGKSASSLEGLCKGLLAEQRASWPELRQAY
                     DSLRGTRYRDMACNGFSVRLLFNPGRAVNTTAAVSPEEIRERPCFLCVANLPAGQRYI
                     LYRREFLILGNPRPVVPFHLTIAHLDHQPQAIAGHMEAFLRIAADLGPGFTTLYNGPR
                     CGASAPDHLHFQAVPSGQMPIEKEIDGTKGSTLPSLTAGVSVFRMAGLGREIILIEGG
                     DARPVADAFEHYVRALKDGLAPPGQPGEEPMLNAAASFDGKTWRLLVFPRRAHRPAAF
                     YRKDDGKVLVSPAIMEMAGVIVTPAERDFDRLDAGAIESLYREVSLSHD"
     gene            3673..>4354
                     /locus_tag="PKN85_04215"
     CDS             3673..>4354
                     /locus_tag="PKN85_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT43641.1"
                     /translation="MQELTREISLLKDKIRELEKSEAHHKKVVEELGSSLQQLRLLVD
                     AGPDFFFFKDLDLTYRLVNLANAKFFGLTEAEIIGKNDYDLMPGEAAVACQQSDRLAI
                     TEKRTIITTEPVGGRFYETYKFPFLVRGEVAGVAGIVRDITERKLAEEELRKSRALLS
                     DMIENSGALICVKDRDGRYEMVNRKWQEVTGISRENTIGRTDRELFPGLTGIVFRRND
                     LEVMESGSV"
BASE COUNT          902 a         1408 c         1228 g          816 t
ORIGIN      
        1 aggcaggtcg gtcatcgtca tcgactacga ccccgggagg acggaaaacc gctactatgc
       61 cgatatagcg cgttacctgc gaagcatgcg cgttcccgtc cccgaggtga cgggccatga
      121 ccctgaggcc cacctcatgg tgctggaaga cctgggaagc aaagacctcc acgccttcgc
      181 cggagaggat tggccgacgc ggcgcgccct ttatcagaag accctcgcgg cggcaaagag
      241 gctccatggc atccaggaaa aggactttcc ttcaggaacc gtgaagctta tggacggttt
      301 cggtccggag ctatacctct tcgagcagaa ctacttcctc gagcatttcg tcaggagcca
      361 ttgcagcgta gacctcgaac ccgccttcga gtcacgactc atgtcggaac tctcccgcct
      421 gacagacgag ctctgctccc tgcggcgctg cctcgttcac cgcgacctcc agtcccagaa
      481 cgtcatggtc ctcggttcgg aaccgtactt catcgatttc cagggcatgc gcttcggaaa
      541 ccccttctac gaccttggct ccctcctgtg tgacccctat gtcaccttca cccccggaca
      601 aagggaggaa ctcctgtcgt tctactacat tctgtcatcc caggaactcg actgggacgc
      661 cttccaggcg tccttctggt ccgcctcggt ggaacgtctc atgcaggccc tcggggcata
      721 cgggttcctc ggccgggcga aaggcctcac agcctttctc ggccacatcc cggcgggtct
      781 tcggaatctc tccatggcag cctcccacgt tccctggctc tccaccctgc gcgaactcac
      841 ggcggaatgc tcggtggcac tgggctgaga gataggacct acgggcggag aataggacca
      901 ataggaggta taggacatgg gtctcatatg tcctattggt cctattttcc tataggtcct
      961 atcctccccg tcccctgtcc cctgcttcaa ggtccctgtc ctcttggtcc ccctttcctg
     1021 tttccttacc tctctttcca tggtaggata tggcactcat gagaccagtt accgccgtct
     1081 gcagctacac tgcgggaccc atgttcctgc cggcgctgag aaacctggcc gcgtccgacc
     1141 ttatcgaaga ggtgttggtt gtggcccggg agcccatacc ggacatcctt ccccgatgca
     1201 cggtcatcaa caccggtccc tttgcctccc gggagacctt cgaaacgatc ctggcaaaga
     1261 ccgcgacgga gtatcttctg ttcatccccc ggaccatccc gatgctcgcc ggcatggcaa
     1321 cactggagag gtttatccgc gcggcccggt ccacgggagc cggcaccgtc tattccgact
     1381 tccatgacga cacgggaaag gggaagacac tccatcccct catcgattat cagcccggca
     1441 gcgtgcggga tgatttcaat ttcggcgccc tcgcccttct tgacgcctcg atcgtccgcg
     1501 acacgcttca aaggtacgga cgcacaccgc ccctgcgcca cggggccctg tacgatattc
     1561 ggctgaagat gtccatagac catgggatcc accatatcgc ggaaccgctc tacagtgtcg
     1621 tggtccacgg tgacactccc ggggcagatg cacacttcgc atacgtcgac ccccgcaacc
     1681 aggcccttca gagggaaatg gagacggtct tcaccgatca cctcaagagg atcaacgcgt
     1741 accttcctcc ggacaggctc aagacaatgg aagaaccggc ggagcccttt cccgtgagag
     1801 cctccgtgat aatccccgtg aagaaccgta gaaagaccat agcggacgcc ctccagagcg
     1861 ccctctcaca ggaaacggac ttcaccttca acatccttgt cgtcgacaat cattcgacgg
     1921 acgggaccac ggaggtcatc gccgacatgg caggcaggaa cccggccata cggcacatca
     1981 tcccccgccg gaaagacctg gggatagggg ggtgctggaa cgcggccatc atggacgacg
     2041 cctgcggccg ctatgccgtc cagctcgact ccgacgacct gtacgagaac caccttgttc
     2101 tcgagaccat cacggatatc atgcgcgacg ggagatacgc aatggtcgtc ggatcctata
     2161 ccatcgtcaa cgagcggctg gagcagatac ctcccggact gatagaccat cgtgaatgga
     2221 cggccgacaa cggacacaac aacgccctca gagtcaacgg cctcggggct ccgcgggcat
     2281 tctccacgac ggccatccgg cggataggtt tcctcaacgt gagctacgga gaggactatg
     2341 ccgccgcgct gaggatgtcc cgggaataca ggatcggaag gatctatgaa agtctctacc
     2401 tgtgccgccg ctggcagggc aacaccgatg ccgggctttc cctcgaagcg gcaaacagga
     2461 acgacgcctt caaggactcc ctccgaaccg ccgagctcgt cgagcggcag aagctggcag
     2521 gggagtcccc ctgagtgaca cgcgcgttga gagaccggac ctacgcgacc ttcgaagggg
     2581 gaaaaagcgc ttcctcactc gagggattgt gcaaggggct tcttgccgaa cagcgggcat
     2641 cctggccgga gctccggcag gcctatgatt cccttcgcgg gacgcggtac cgggacatgg
     2701 cgtgcaacgg attctcggtc cgtctcctgt tcaatcccgg cagggccgtc aacacaacgg
     2761 ccgccgtaag ccccgaagag atcagggaac gaccctgctt cctctgcgtc gccaacctgc
     2821 ccgcaggaca gagatacatc ctctaccggc gcgagttcct gatcctggga aacccccggc
     2881 ccgtggtacc ctttcacctc accatcgctc acctcgatca tcaaccccag gccattgccg
     2941 gtcacatgga ggcctttctg cgcatagcgg ccgacctcgg tcccggtttt acaaccctgt
     3001 acaacgggcc tcgctgcggg gcttcagcgc ccgaccatct ccatttccag gcggtaccct
     3061 ccgggcagat gcccatagaa aaggagatcg acgggacgaa gggctccact ctcccgtcgc
     3121 tgactgccgg cgtatccgtg ttcaggatgg ccggcctcgg gcgcgagatc atcctcatcg
     3181 aagggggcga cgcccggcct gtggcagacg ccttcgagca ctacgtgcgc gccctcaaag
     3241 acgggttggc accccctggt cagccgggcg aggaacccat gctgaacgct gctgcttctt
     3301 ttgatggaaa gacgtggcgc cttctcgttt ttccccgccg cgcccaccgc ccggccgcct
     3361 tctacaggaa ggatgacggg aaagtcctcg tcagcccggc gatcatggaa atggcgggcg
     3421 tcatcgtcac ccccgcggag cgtgatttcg accgcttgga cgccggggcg atagagtccc
     3481 tgtatcgcga ggtctctctc agccacgact gaacacggcc taaacctctg atcccgtagt
     3541 ggcgataaga tatctggagg tcagcacgat accaggcgcg gcactcaagg aacaccgccg
     3601 cggattccat attgatatac ccgcgtcata tctattatta ttgttcaagg gagaggacaa
     3661 acgttgaaga cgatgcagga attgacccgg gagatctccc tcttaaagga caagatccgg
     3721 gaactggaga agtccgaggc acaccacaag aaggtcgtgg aggaactggg gtcatccctg
     3781 cagcagttgc gcctcctcgt cgatgcggga ccggacttct tcttcttcaa ggatctcgac
     3841 ctcacctacc ggcttgtcaa cctggcaaac gcgaagttct tcgggctcac cgaggccgag
     3901 atcataggca agaacgatta cgatctcatg cctggcgaag cagcggtcgc ctgccagcag
     3961 agcgatcggt tggccatcac cgaaaaaagg acgatcatca ccaccgaacc cgtcgggggc
     4021 aggttctatg agacctacaa gttccccttc ctcgtcaggg gcgaggtggc aggtgtcgcg
     4081 gggatcgtcc gcgacatcac ggagcgcaag ctcgccgagg aggaactgcg caaaagccgt
     4141 gccctcctgt cggacatgat cgagaacagc ggggccctca tctgtgtcaa ggaccgtgac
     4201 ggccgttatg aaatggtgaa ccgcaagtgg caggaagtga ccggcatcag ccgggaaaat
     4261 accatcggca gaacggaccg ggaactcttt cccggactga cggggattgt ttttcgccgg
     4321 aatgacctgg aggtcatgga atcaggatcg gtga
//
LOCUS       DAOQTR010000127         4329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22689_length_4329_cov_3.455545, whole genome shotgun sequence.
ACCESSION   DAOQTR010000127 DAOQTR010000000
VERSION     DAOQTR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22689_length_4329_cov_3.455545"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="amrA"
                     /locus_tag="PKN85_04220"
     CDS             <1..768
                     /gene="amrA"
                     /locus_tag="PKN85_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNT43642.1"
                     /translation="VAGLDAAGLLEGLAKKENELCGAPAVVASMMAATKMGAEAEVLH
                     YTNSGDTAGDRTRVVGYGSLAFWQPDGKAASSPLAAAERKRLLAIARKTLEEYVSSKT
                     VPEIDVKDPRLLERRGVFVTLTIHGQLRGCIGYIKPVAPLYRSVVDMTVAASSRDMRF
                     RPVTKGELKDIEIEISVMSPLKLITSVDEIKVGIHGLYIVKGGNAGLLLPQVATQYKW
                     GREEFLQNTCRKAGLPENSWKDKETKVYVFSAQIFSE"
     gene            807..1178
                     /locus_tag="PKN85_04225"
     CDS             807..1178
                     /locus_tag="PKN85_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43643.1"
                     /translation="MKISFVVPTGDIVNEERDLFLTHAMGGCWHEDGGGAPVTKFALK
                     GHICSKCGLFYTTRNDFSTLEDFLKLYEWARGDPGLEDFVAQFRTRDLVNEKRGPGAR
                     KRFADGLYRILTARGRETGNV"
     gene            1171..2373
                     /locus_tag="PKN85_04230"
     CDS             1171..2373
                     /locus_tag="PKN85_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNT43644.1"
                     /translation="MFDSDTLDRYADVLLWGLTAARKGRFRKGDVIFLQYDPPATTLA
                     EVLYGKILDRGMYPLQRMGLTTFMEHAFYEKATDRHLGWLPPGDRELYEKVNGRIYLR
                     APESLTHLKDIDPMRISKVLVSRKVFRDILDKREEKGVYSWTLCTYPTAGLAKQAKMS
                     VAAYARQIVKACFLDEKDPVRRWDAVHAEATRIKKWLTGLKVRYLRVNSKNIDLVLTP
                     GENRKWAGISGHNIPSFEVFLSPDWRGTEGTYYANLPSFRSGNYIEGVRLVFRKGKVV
                     EADAKKGAEFLRKQVAMDKGASRVGEFSLTDKRFSLIDRFMADTLFDENFGGSEGNCH
                     LALGASYSDTFSGNPAKLTEEMKHKLGFNDSALHWDLVNTEAKTVTARLAKGREIVIY
                     EKGAFCLP"
     gene            2415..3536
                     /locus_tag="PKN85_04235"
     CDS             2415..3536
                     /locus_tag="PKN85_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT43645.1"
                     /translation="MVLYFTQRWIFGPLIPSLMEAFGTDKTTLGIVGAGSLWGYMLTP
                     IIAGLISDRFGRRYVVLFGMFGLSAMTVVSGLAHSTGQLFLFRFVTGLIEGFFFIPMI
                     AFTMELFPERPGFYVTFMSSGTSFGWFTGPALAGWLLDLTGNWRIPFMTTGIAGMILA
                     AVLLAVWPEEKRERHPGGKLLDPSILTRSSLIMLVLVSCTAMFQISGEFGFAMWYPVF
                     LKTEMGMTASMAGLVAGFWGVGQFIGRPTMGHISDRLGYRKVGITGGIIMGLCLMLIL
                     KIDNPVARGSITFITGFVGCAVMGSLWTFTGLTFARTRGLALGVLTTWSYAIASLSPI
                     TIGWIGDHYRVSTGLFVICVPVVFLACATFAATNLVKKQ"
     gene            3712..4155
                     /locus_tag="PKN85_04240"
     CDS             3712..4155
                     /locus_tag="PKN85_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43646.1"
                     /translation="MFVFHSKSPQKESRAMGKAIPVNLLRMWELLSEDIQDALLITVE
                     KNRAAHRNFRGTPVSSCPHCGETDTMDCSTIDAVGDATVGLCLVCGYLWCLECDSSLL
                     TGINCGHWQVCSSCTEKKQSTGYCGTEPLECPRIREWLRKNHPVV"
BASE COUNT          903 a         1279 c         1274 g          873 t
ORIGIN      
        1 gtggccggcc tcgatgcggc gggcctcctt gagggactgg cgaagaaaga gaacgagctc
       61 tgcggagcgc cggcggtggt cgcctccatg atggcggcga cgaagatggg ggctgaggcg
      121 gaggtccttc actacacgaa ctcgggggat acggcaggtg acaggacgag ggtggtgggt
      181 tacggttccc tggccttctg gcaacccgat ggtaaggccg cctcctcacc gcttgccgcg
      241 gccgagagga agcggctcct tgccatcgct cgaaagaccc tcgaggaata cgtgtcgtcg
      301 aagaccgtgc ccgagatcga tgtcaaagac cccaggctcc tcgaaaggag aggggtcttc
      361 gtgacgctca ccattcacgg gcagctcagg ggatgcatcg gctacatcaa gcccgtggct
      421 cctctctaca ggtcggtggt ggacatgacc gtcgcggcgt cgtcgaggga tatgaggttc
      481 cggcccgtca caaagggcga gctcaaggat atcgagatcg agatctccgt gatgtcaccc
      541 ctgaagctca tcacctcggt ggatgagatc aaggtgggga ttcacggtct gtatatcgtg
      601 aaagggggca acgcgggcct gctgctgccg caggtggcca cccagtacaa gtggggccgg
      661 gaggaattcc tgcagaatac gtgtagaaag gccggcctgc cggagaactc gtggaaggac
      721 aaggaaacga aggtctacgt tttttcggcg cagatattct cggaataagg gatgttcagc
      781 ggtcaaccgc tggtaaggga gcacacatga agatcagttt cgtcgtgccc acgggcgaca
      841 tcgtcaacga agagagggac ctctttctca cgcatgccat gggaggctgc tggcatgagg
      901 acgggggcgg ggcacctgta acgaagttcg ccctcaaggg ccacatctgc tcaaagtgcg
      961 gcctgttcta cacaacgaga aatgatttct ccaccctgga ggatttcctg aaactctacg
     1021 agtgggcgcg cggcgacccg ggccttgagg atttcgtcgc acagtttcgg acccgggacc
     1081 ttgtcaacga gaaacgaggg ccgggagcaa gaaaaaggtt cgccgacggt ctctaccgga
     1141 tactgacggc acgggggagg gaaacgggaa atgtttgaca gcgacacact cgacaggtat
     1201 gccgacgtgc tcctctgggg gctcaccgcg gcccggaagg gacgtttcag gaaaggggat
     1261 gtcatctttc tccagtacga ccccccggca acaacccttg cggaggtcct ctacgggaag
     1321 atcctcgacc ggggaatgta tcctcttcag cgcatggggc tcaccacctt catggagcac
     1381 gccttctacg agaaggcaac ggaccggcac ctcggctggc tgccgccggg ggacagggaa
     1441 ctatacgaga aggtcaacgg gaggatatac ctgcgcgccc ccgagtccct cacccatctt
     1501 aaggacatcg accccatgcg gataagcaag gtcctggtat cccgaaaggt ctttcgcgac
     1561 atcctcgaca aaagggagga gaaaggggtc tacagctgga cgctgtgcac ctatcccacc
     1621 gccgggcttg cgaaacaggc gaagatgtcc gtcgcggcct atgcccggca gatcgtcaag
     1681 gcctgctttc tcgacgagaa ggaccccgtc aggagatggg acgccgtcca cgccgaggcg
     1741 acacgcataa agaaatggct cacgggcctc aaggtgcggt atctccgggt caactcgaag
     1801 aacatcgacc tcgtcctcac accgggggag aaccgcaaat gggcgggcat ctcggggcac
     1861 aacatcccca gcttcgaggt cttcctctca cccgactggc gaggcaccga aggaacctac
     1921 tacgccaacc tgccatcctt ccggagcggc aactacatcg agggcgtgcg cctcgtgttc
     1981 aggaagggaa aggtcgtcga ggcggacgcg aagaagggcg ccgaatttct aaggaaacag
     2041 gtcgccatgg acaagggggc ctcccgggtc ggggaattct cccttacgga caagcgcttt
     2101 tccctcatcg accgcttcat ggccgacacc ctcttcgatg agaacttcgg cggctccgag
     2161 gggaactgcc acctcgctct cggggcgtcc tacagcgaca ccttcagcgg caacccggcc
     2221 aaactgacgg aggagatgaa gcacaagctc ggcttcaacg actccgcgct tcactgggac
     2281 cttgtcaaca ccgaggccaa gaccgtgacg gcacgccttg cgaagggcag ggaaatagtg
     2341 atctacgaga agggtgcctt ctgtcttcca tagggtcgca taagcacctc ttcttctttg
     2401 tcggctgggt ggggatggtc ctctacttca cccagaggtg gatcttcggg ccgctgatcc
     2461 cctccctcat ggaagccttc ggcaccgaca agaccaccct cggcatcgtc ggcgcgggct
     2521 cgctgtgggg ttacatgctg acaccgatca tcgccggtct catatccgac aggttcggga
     2581 ggagatacgt ggtcctcttc ggcatgttcg ggctctcggc gatgactgtc gtctcgggcc
     2641 tcgcccactc cacggggcag ctctttctgt tccgtttcgt gacgggcctc atagaaggct
     2701 tcttcttcat ccccatgatc gctttcacga tggaactctt tcctgaaagg cccgggtttt
     2761 acgtcacctt catgtcatca ggcacgtcct tcggctggtt caccggaccg gccctcgcgg
     2821 gttggctcct cgaccttacc ggaaactggc gcatcccctt catgaccacg ggcatagccg
     2881 gcatgatcct cgccgccgtg ctccttgccg tatggcctga agagaagcgg gagaggcatc
     2941 ccggcggaaa actcctcgac ccgtccatac tcacgcggtc gagccttatc atgctcgttc
     3001 tcgtgagctg caccgcgatg ttccagatat cgggggagtt cggttttgcc atgtggtacc
     3061 ccgtctttct gaaaacggag atgggcatga cggcttcgat ggcaggcctt gtggcgggct
     3121 tctggggtgt cggccagttc atcggccgcc cgacgatggg ccacatctcc gacaggctgg
     3181 ggtaccgtaa ggtcggcatc accggcggca tcatcatggg attgtgcctt atgctgatcc
     3241 tcaagatcga caatcctgtc gccagaggct ccatcacgtt catcacgggt tttgtgggct
     3301 gcgccgtgat gggctccctc tggacattta cgggcctcac cttcgcccgc acgaggggcc
     3361 ttgcccttgg cgtgctgacg acgtggtcct acgcgatcgc ctctctctcc cccataacca
     3421 tcggctggat cggcgatcat taccgcgttt ccacgggcct tttcgtcatc tgcgtccccg
     3481 tggtcttcct cgcctgcgcc accttcgcgg caacaaacct tgtcaaaaaa caatagtcaa
     3541 taaccaaact ccaatgacca gataatctcc aataaatcaa taatcaatga ccagacagaa
     3601 gacggggggg aatcaaaacg gtccgctctg tccttattct ttggtcaatt ggctattggt
     3661 gaatcggtca ttatgttctc atttggttcc tgttccattt gtgatacaat aatgttcgta
     3721 ttccactcca aatctcccca gaaggagtct cgagccatgg gcaaggcgat accggtcaat
     3781 ctcctgagaa tgtgggaact cctctccgaa gacattcagg acgcgctcct catcaccgtc
     3841 gaaaagaacc gcgcggcaca caggaatttt cggggaacac cggtctcttc ctgcccccac
     3901 tgcggtgaaa cggacacgat ggattgctcc accatagacg ctgtcggcga cgcgaccgtg
     3961 ggcctgtgcc ttgtctgcgg gtacctgtgg tgcctggaat gcgattcgtc cctcctcacc
     4021 ggtatcaatt gcggccactg gcaggtctgt tcatcatgca cggagaagaa gcagtccacc
     4081 ggctactgcg gcaccgaacc cctggaatgc ccccgcatca gggagtggct gaggaaaaac
     4141 catccggtgg tttgaagacg gattgaaccg cttgaacgtc agaggcaaaa aaaaatggga
     4201 cactgtcgtc atcccggttt tgatccggag atcctcttcg tcttcgtcat tcaagcggtt
     4261 acaaccattt ttgtcttgac ctatgatgcc tttagtggac accaaagttg cggtatacta
     4321 accacacgg
//
LOCUS       DAOQTR010000128         4296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22927_length_4296_cov_3.832822, whole genome shotgun sequence.
ACCESSION   DAOQTR010000128 DAOQTR010000000
VERSION     DAOQTR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22927_length_4296_cov_3.832822"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="PKN85_04245"
     CDS             <1..1536
                     /locus_tag="PKN85_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43647.1"
                     /translation="TRRQAEETLHLTNVYNKNLIEANLDPLVTIDLEGKVSDVNSATE
                     LITGYSRADLIGTDHADCFTQPERARAGYRLVLEKGLVRDYPLEIRHRSGRSTSVLYN
                     SSLYRDETGKIIGVVATARDITEKNRIERHLRETHKMEAIGTLAGGIAHDFNNIIAGI
                     MGFAEMVLDDIPEDTPAHRRLGYVLKGAHRGRDLVKQILAFSRRSEQEKKVISLDQII
                     DEVVTLVRATLPATIEIRRSNTARSDLVSADPSQIHQVVVNLCSNAAHAMSEKGGAIE
                     ISLSEEIVSSAEGSPHHDLSPGSYVRLTVSDNGRGMEPATLERIFEPFFTTKPPGEGT
                     GLGLAVVHGIVRDHDGVITASSRPGGGSSFSIFIPQIRTGEADEIERIEAALEGPESI
                     LVVDDEEFIVEMSTQRLQRFGYRATGATSSAEAIELFRADPGGFDLAVIDHIMPDMTG
                     LELAKALLAIDPGIRIILCSGLNEPIPMERMRQAGIRDFFTKPADRNEFIRIVRNVLD
                     SPP"
     gene            1701..2498
                     /locus_tag="PKN85_04250"
     CDS             1701..2498
                     /locus_tag="PKN85_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT43648.1"
                     /translation="MKRYLIFVAAAMCLIFSGTAPASETLVASGHPEYAPIMWKEGNN
                     IVGAGPELVKLLFKDLNVTVKTPYSGDWSKVQQEAKEGRVDLIVGLYLTEDRKAFFQY
                     SNPYAKDPVVAFVAKGKGFPYSKWDDLIGKKGTTTVGDSFGQVFDRFLAEKLSVTRSA
                     TVEESFSKLLKGRADYFIYAMYSGLFEADKMGITNKVELLSKEICVENWYMGISKKSP
                     YVKYLPQINKKLDELINNGTVDRLIEKYGSQYRKSIAKKKGSPAKAK"
     gene            complement(2616..3629)
                     /locus_tag="PKN85_04255"
     CDS             complement(2616..3629)
                     /locus_tag="PKN85_04255"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HNT43649.1"
                     /translation="MAFTVTTSRSFLARCSVPLIVFVLLFSSIAVGSSAGAFEEDVSA
                     PLTPSFSVGGKIRYLFKSHTSYEFGNPFPPCQEPLSRLEFPLDTWWAGLDMRLSFPRF
                     SLGLETLTSVKQNAHGEFEDSDWDDDARPDEKTTYSTSQMRVAPSYMVRFDADLEVSD
                     WLGLPGWLKIRPLVGIRYQRFNLVSHDGIQYDLTGASGNTLLPGEGIRFKQTYWHYFV
                     GLRSVIDLSRPTGIPSLSLNLQADWAYVEGMNEDNHLFRPGRRFTYEDTYGEAWHCSM
                     GLKKELGEGFSVGLDGDYLTVSTTGNHRLLNRTFGIDMSFSNGVKVWSDQLSLSLTLE
                     YRF"
     gene            3903..>4296
                     /locus_tag="PKN85_04260"
     CDS             3903..>4296
                     /locus_tag="PKN85_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HNT43650.1"
                     /translation="MTSATLKLLACALMVVDHLGSIFFPHEPVMRAIGRLAYPMFAYL
                     IAEGYSHSKDKTSYLGRLFLFSLVSQPFYMFAFRYPVVHFNVFFTLAAGLYAIYAYEK
                     KKSLVPVLLAGISLEFVKASYGLPGALMI"
BASE COUNT          978 a         1239 c         1257 g          822 t
ORIGIN      
        1 acccgcaggc aggccgaaga gacccttcac ctgacaaacg tctacaacaa aaacctcatc
       61 gaggccaatc tcgaccccct cgtcaccatc gacctcgagg ggaaggtcag cgacgtcaat
      121 tcggcgacgg agctgataac ggggtattcc agggcggacc tcatcgggac ggaccatgcg
      181 gattgcttca cgcagccgga aagggcacgc gcgggctatc ggcttgtcct ggaaaaaggt
      241 ctcgtgcggg actacccgct ggagatacgg cacaggtccg gccgcagcac gtcggtgctg
      301 tataattcat ccctgtaccg ggatgagacg ggaaagatca tcggcgtcgt ggcaacggca
      361 cgggacatca cggaaaagaa caggatcgaa aggcacctga gggaaacgca caagatggag
      421 gccatcggca ccctcgccgg gggcattgcc cacgatttca acaacatcat cgccggcata
      481 atgggcttcg ccgaaatggt cctcgacgac atccccgagg atactccggc gcaccggagg
      541 ctgggctatg tcctgaaagg ggcgcatcgg ggccgtgacc tcgtcaaaca gatcctcgcc
      601 ttcagccgca ggagcgagca ggagaagaag gtcatttcgc tggaccagat catcgacgag
      661 gtcgtcacac tcgtgagggc aacccttccc gctaccatag agatcaggag aagcaacacc
      721 gcaagatccg acctcgtgtc cgccgatccc tcgcagatac accaggtggt ggtaaatctc
      781 tgctccaatg ccgcccatgc gatgagcgaa aagggtgggg ccatcgagat atccctttcg
      841 gaagagatcg tttcgtccgc ggaagggtcg ccccatcacg acctttcgcc gggctcatac
      901 gtgaggctca cggtgagcga caacggacgc ggcatggagc cggccacgct ggaacggata
      961 ttcgagccct tcttcaccac caagcccccc ggggagggga cgggactggg tctcgccgtc
     1021 gtccatggga tcgtgaggga ccacgacggt gtgatcacgg catcaagcag gcctggagga
     1081 ggctcgtcct tcagcatctt tatcccccag atcaggacgg gtgaggccga tgagattgag
     1141 aggatcgagg cggccctgga aggaccggag tcgatccttg ttgtcgatga cgaggaattc
     1201 atcgtcgaga tgagcacgca gaggcttcag cgcttcggct accgggccac gggcgccacg
     1261 agcagcgccg aggcgataga actctttcgc gccgacccgg gcggtttcga cctcgccgtc
     1321 atcgaccaca tcatgcccga catgaccggg ttggagctgg caaaggcgct gctcgccata
     1381 gacccgggca tccgcatcat cctgtgctcc gggctcaacg aacccatccc gatggagagg
     1441 atgaggcagg cgggcatcag ggacttcttc acgaagccgg cagacaggaa cgagttcatc
     1501 cggatagtgc ggaacgttct cgacagcccc ccgtagcaaa cctccccgga tgtccctgct
     1561 cccttcccgg gaatgcggat cgtataccgc aaaaccacaa aacgacagat atcggcgaat
     1621 ttgtttttta acgagggctg gattgtggta gtatttcttc actttcggta aggcgcattc
     1681 ttctgggaaa ggggtactgc atgaaacgct atcttatctt tgttgccgcg gcaatgtgcc
     1741 ttatattctc cggcactgcc ccggcgtcgg aaacactcgt agcctcaggg catcccgagt
     1801 atgcacccat aatgtggaaa gagggcaaca acatagtggg cgccgggccc gaactcgtga
     1861 agcttctctt caaagacctc aacgtcacgg tgaaaacacc ctacagcggg gactggagca
     1921 aggtccagca ggaagcgaag gaaggcaggg tggacctcat cgtggggctg tacctgacgg
     1981 aggacagaaa ggccttcttt cagtattcca atccctacgc caaggatccc gtggtggcgt
     2041 tcgtggccaa gggcaaaggg tttccctatt cgaaatggga cgacctcatc gggaagaagg
     2101 gtaccacgac cgtcggcgac agcttcggtc aggtcttcga caggttcctt gccgagaaac
     2161 tctccgtcac ccgctcggcg acggtggaag aaagcttttc aaaactcctg aagggccgtg
     2221 ccgactattt catctacgcc atgtattccg gccttttcga ggcggacaag atgggaataa
     2281 cgaacaaagt ggagctcctt tcgaaggaga tctgcgtgga gaactggtac atggggatct
     2341 ccaaaaagtc tccctatgtg aagtaccttc cccagatcaa caagaagctc gacgaactca
     2401 tcaataacgg aacggtggac aggctgatcg aaaaatacgg cagccagtat aggaaatcga
     2461 tcgctaagaa gaaggggagc cctgcgaagg ccaagtagca gggtagtggg ggtaggaact
     2521 cacaatccag gaatgcgccg cgcggctggt ccatcgtgca ttccctttgg caacgcggcg
     2581 cattccctgg acaaacgtca tgaagagatc tccgatcaaa agcggtattc cagtgtgagc
     2641 gacaggctca actgatccga ccacaccttg accccgtttg aaaaactcat gtcgatgccg
     2701 aaggtccggt tcagcaggcg atggttgccc gtggtggaca ccgtcaggta gtcgccgtcg
     2761 aggcccactg aaaaaccctc acccagttcc tttttcaacc ccatcgagca atgccaggcc
     2821 tcgccgtagg tatcctcgta cgtgaaccgc ctgcccggcc tgaacaggtg attgtcctcg
     2881 ttcatgcctt ccacgtaggc ccagtcggct tgcagattga gactcaggga aggtatgccc
     2941 gtcggccgcg aaaggtcgat gacggagcgc agaccgacga aatagtgcca gtacgtctgc
     3001 ttgaaacgga tgccttctcc agggaggagg gtgtttcccg acgctccggt cagatcgtac
     3061 tgtataccgt cgtggctgac aagattgaac ctctgatacc gtatccccac gagaggcctt
     3121 atcttcagcc agccgggcag gccgagccag tcggagacct ctaggtccgc gtcgaaacgc
     3181 accatgtagc tgggagcgac acgcatctgc gatgtgctgt aggtggtctt ttcgtcggga
     3241 cgggcatcgt cgtcccagtc ggagtcttcg aactcgccat gggcgttctg cttcacgctg
     3301 gtgagggtct ccagcccgag tgaaaagcgg gggaagctca atctcatgtc gagcccagcc
     3361 caccaggtat cgaggggaaa ttccagccgg ctgagcggtt cctggcaggg gggaaaggga
     3421 ttgccgaact cgtaggaggt atggctcttg aaaagatacc tgatcttccc cccaacggag
     3481 aatgacggcg tcaggggagc ggaaacatcc tcttcgaaag ccccagccga cgacccgacc
     3541 gcgatagagg agaacaggag aacaaacact atcagcggaa cggagcatcg ggcgaggaac
     3601 gaacggcttg tcgtaacggt gaatgccatg tcgttgaacg ctcatgttac cacatcatcc
     3661 ccggcgattg tcgtctttat ttgccgggag acaatgaaaa atgtttctcc ataaaggcaa
     3721 atgtgctaca aggggaagga cgggttccgg cagatggcgg tacgaaggtc ccatgcgggc
     3781 gggtcaacgg gatgagatgc tgccgggcgg agagcgagga ccaacggccc tgtcttaagt
     3841 ccggaggcaa gcttccgaaa tatctttgtg gcatttgctt cacgatcaag gagatacagg
     3901 atatgacctc agctacactg aagcttctgg catgcgccct catggtggtc gaccacctcg
     3961 ggtccatctt cttcccccac gaaccggtga tgcgggccat cggcagactg gcctatccca
     4021 tgttcgccta tctcatcgcc gagggctata gccactcgaa ggacaagacc tcctatctcg
     4081 gccgcctctt tctcttctcc cttgtctccc agcccttcta catgttcgcc ttccgctatc
     4141 ccgttgtgca tttcaacgtc ttcttcaccc ttgccgcggg gctttacgcg atatatgcct
     4201 acgagaagaa gaagagcctc gtgcccgttc ttctcgccgg catatccctg gagttcgtga
     4261 aggcctccta cggcctgccg ggggccctca tgatct
//
LOCUS       DAOQTR010000129         4294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_22942_length_4294_cov_3.402689, whole genome shotgun sequence.
ACCESSION   DAOQTR010000129 DAOQTR010000000
VERSION     DAOQTR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22942_length_4294_cov_3.402689"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="tgt"
                     /locus_tag="PKN85_04265"
     CDS             <1..1066
                     /gene="tgt"
                     /locus_tag="PKN85_04265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNT43651.1"
                     /translation="ETPVFMPVGTQGVVKAMTHRQLEELGVTMILSNAYHLYLRPGDE
                     LIRRMGGLHNFSGWDGAILTDSGGYQIFSLGVLREIKEDGVLFQSHIDGSRHFLTPEK
                     IVHVQENIGADIAMCLDECVSYPSTFEYTRESVELTTRWARRCLAARSGEGSQKLFGI
                     IQGGFYPDLRLRSAEDILSLDFDGFAIGGLSVGEPKSLMWDMVDTVIGLMPAGKPRYM
                     MGIGLPEDLLEGVKRGVDMFDCIIPTRFARNGGLFTWDGRINIKNARFTEDEGPIQEG
                     CTCYTCRTFSRAYLRHLTVSHELTSFYLNTIHNVHFYMEFMKKIRSAVSTGTFDGFYH
                     DFKRRYEGGESDDRYSVCNG"
     gene            1038..1358
                     /gene="yajC"
                     /locus_tag="PKN85_04270"
     CDS             1038..1358
                     /gene="yajC"
                     /locus_tag="PKN85_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNT43652.1"
                     /translation="MTGIAYAMGSNPAGGAGQGGGSYMSLIFIAAVFAIFYFILIRPQ
                     QKRAKQHKDFLENLKKGDKVITSGGLYGTITGISDDSVTIEIAEKVRVKVDKGTVVQY
                     QKTQ"
     gene            1355..1723
                     /gene="rpsF"
                     /locus_tag="PKN85_04275"
     CDS             1355..1723
                     /gene="rpsF"
                     /locus_tag="PKN85_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNT43653.1"
                     /translation="MKGDVNVGRYENIVVLSPDLAKEEEDDLMKRIQGNLERAGATIV
                     KLDDWAVRKLAYPIKKKDKGHYFFYLLDMDEGSVATMAKFYRTLDPVLRHMFVVVDDK
                     NEGPKKPPDPVVFDELEGEF"
     gene            1723..1992
                     /locus_tag="PKN85_04280"
     CDS             1723..1992
                     /locus_tag="PKN85_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43654.1"
                     /translation="MNRVFMTGKVEAMPRVVYTPKGDRFVLFPLRVGEGDFLIDVECP
                     GDPAMPRPDAARGRSVMVSGMLVRKKLRSREMIQLKAHKIFWMEE"
     gene            2005..2259
                     /gene="rpsR"
                     /locus_tag="PKN85_04285"
     CDS             2005..2259
                     /gene="rpsR"
                     /locus_tag="PKN85_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNT43655.1"
                     /translation="MRTTRSDSPKKRVYVRRRVCRFCQDSNIVIDYKDPKLLKSFITE
                     RGKIMPRRMTGTCANHQRKLKEAIKMARHIAFLPFTTLLK"
     gene            2272..2724
                     /gene="rplI"
                     /locus_tag="PKN85_04290"
     CDS             2272..2724
                     /gene="rplI"
                     /locus_tag="PKN85_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNT43656.1"
                     /translation="MKLILIEDVQGTGKKGDTVTVKDGYGRNFLVPRGLAVPATEVNV
                     VRFENIVRSVSNKKARDLETASELKAKLEEATLTIKKKAGEDGRLFGSVTHKDIVEAV
                     KAVSGVEVDKKLVRLEEPIKTTGSHTVVIHLQPDVNAEVKIEVEKEEA"
     gene            2725..4116
                     /gene="dnaB"
                     /locus_tag="PKN85_04295"
     CDS             2725..4116
                     /gene="dnaB"
                     /locus_tag="PKN85_04295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNT43657.1"
                     /translation="MARVLKNARDVTGPAAVRVPPQNIEVEQSLLGGLLIDPESMNKV
                     VDVVGPSDFYRDAHGKIYDLMVHLYEKNDAIDIITVSSLAKDRGLLEPIGGVTYLNTL
                     VDLMPSAANVAYYAKIVKEKSLVRTIINVATEIIEKGFNTETDIDTYIDEAEKLIFQV
                     SENKLKPTYLPVKDFVMDNIKTIERLYEKKQTVTGVPTGFIDLDRLTSGLQPSDLIIV
                     AGRPSMGKTSFSMNIAQHVSMLKENPIPVGIFSMEMSKEQLVTRLLSSESEIEHSKLR
                     TGTFSREEWPKLVRAAGKLSEAHMFIDDSPALSVLELRARARRLKKEHDVGLLVVDYL
                     QLMRGRSSGGDRREQEISEISRFLKALAKEINIPVIAISQLNRAPETREKENKRPRLA
                     DLRESGAIEQDADVILFIYRDEVYNKDRDDNKGVAEVIIGKQRNGPTDTVKLSFIDKC
                     ATFRNGYYQDDEG"
     gene            4103..>4294
                     /locus_tag="PKN85_04300"
     CDS             4103..>4294
                     /locus_tag="PKN85_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNT43658.1"
                     /translation="MTKGDGMRSMNVPNILSIFRFFVTFYFIYAVYQGRMRLALYLFL
                     IQGVSDLLDGFIARVTGSKT"
BASE COUNT         1031 a         1178 c         1255 g          830 t
ORIGIN      
        1 agagacgcct gttttcatgc ccgtgggcac ccagggtgtc gtcaaggcca tgacccaccg
       61 gcagcttgaa gagctgggcg tcacgatgat cctgtccaac gcctaccacc tctacctgcg
      121 ccccggtgac gagctcatca gaaggatggg cggcctccac aatttctccg gatgggacgg
      181 tgccatcctt accgacagcg gagggtacca gatattcagc cttggggtcc tcagggagat
      241 caaggaggat ggtgtccttt tccagtccca tatcgacggc tcccgtcatt tcctgacgcc
      301 cgagaagatc gtgcacgtcc aggagaacat aggcgccgac atagcgatgt gcctcgacga
      361 gtgcgtctcc tatccctcca cgttcgaata caccagagag tccgtggaac tgacgacgcg
      421 gtgggcgcgc cggtgcctcg ctgcccggtc gggggagggg tcgcagaagc tcttcgggat
      481 catccagggc ggcttttatc cggacctgag gctgcgctca gccgaggaca tcctttccct
      541 cgatttcgac ggttttgcca tcgggggtct cagcgtcggc gaaccgaaga gtctcatgtg
      601 ggacatggtg gacacggtca tcggcctcat gcctgccggc aagccccgct acatgatggg
      661 catcggcctg ccggaagacc ttctggaagg cgtgaagcgg ggcgtagaca tgttcgactg
      721 catcatcccc acgcgctttg cccggaacgg cggcctcttc acatgggacg ggaggatcaa
      781 catcaagaac gcccgcttca ccgaggacga agggcccatc caggaaggct gcacgtgcta
      841 tacatgcagg accttttcca gggcctatct caggcacctc acggtctctc acgagctgac
      901 cagtttctac ctgaacacca tacacaacgt tcatttctac atggagttta tgaagaagat
      961 ccgaagcgcc gtcagcaccg gcacgttcga cggtttttat cacgatttca agagacgtta
     1021 cgaaggaggt gaatcagatg acaggtatag cgtatgcaat gggtagtaat cctgccggcg
     1081 gggccggaca ggggggcggt tcctacatgt cgctcatctt tatcgcggcg gtcttcgcca
     1141 ttttttactt cattctcatc cgtccgcagc aaaaaagggc gaagcagcac aaggacttcc
     1201 tggagaacct gaagaaggga gataaagtta tcacatcggg tggtttatat ggtacaataa
     1261 cgggtatttc cgacgacagt gtcaccattg agatcgccga gaaagtgcgt gtcaaagtcg
     1321 acaagggcac tgtggtgcag tatcagaaga cacaatgaaa ggagatgtga acgtgggaag
     1381 atatgagaat attgttgtgc tcagccctga cctcgcgaaa gaggaagaag acgatctcat
     1441 gaagaggatc cagggcaacc ttgaaagggc gggcgcaacg atcgtcaaac tggacgactg
     1501 ggcggtgaga aagctcgcct atcccataaa gaagaaggac aaaggccatt atttcttcta
     1561 cctgcttgat atggacgaag gcagcgtcgc gacgatggcc aagttctaca ggaccctcga
     1621 ccccgtgctc aggcacatgt tcgtcgtcgt cgacgacaag aacgaagggc cgaagaagcc
     1681 gcccgacccc gttgtgtttg acgagctcga aggcgagttc tgatgaacag ggttttcatg
     1741 acggggaagg tcgaggccat gccccgtgta gtctataccc ccaaggggga cagattcgta
     1801 cttttcccgc tgcgtgtggg tgagggtgat ttcctcatag atgtggagtg tcccggagac
     1861 cccgcgatgc cccgtcccga cgcggcacgg ggccgcagcg tcatggtgtc ggggatgctc
     1921 gtgcggaaga aactgcggtc gcgggagatg atacagttga aagcacacaa aattttctgg
     1981 atggaggaat agatgccgag accaatgagg acaacacggt ccgattcacc gaagaaaagg
     2041 gtctacgtga gaagaagggt gtgcaggttc tgccaggatt ccaacatagt catcgactac
     2101 aaggacccga agcttctcaa atcgttcatc accgaaaggg gcaagatcat gcccagaagg
     2161 atgacgggga cctgcgcgaa tcaccagcgc aaactgaaag aggccatcaa gatggcccgt
     2221 cacatagcct ttctgccctt tacgacttta ctgaaataac ggaggcaacc gatgaagctc
     2281 atactcattg aagatgtcca gggcacaggg aagaagggcg ataccgtgac cgtcaaagac
     2341 gggtacggga ggaactttct cgttcccagg ggactggccg tgcccgcgac tgaagtaaac
     2401 gtggtccgct tcgagaacat cgtgcgcagc gtttcgaaca agaaggcgcg ggaccttgag
     2461 accgcttcgg aattgaaggc gaaactcgag gaagcaaccc ttacgataaa gaagaaggcc
     2521 ggcgaagacg gcaggctctt cggatccgtt acccacaagg acattgtcga ggccgtcaag
     2581 gccgtttcgg gtgtcgaggt ggacaagaag ctggtccgcc tcgaggagcc gatcaagacg
     2641 accggttcac atacggttgt gatccacctg cagccggatg tcaacgccga ggtcaagatc
     2701 gaggtggaaa aagaagaggc ttaaatggcc cgggtcctaa agaatgccag ggatgtgacg
     2761 ggccctgccg cagtgcgggt ccctccccag aacatcgagg tcgagcagtc attgctcggc
     2821 gggcttctca tcgacccgga atccatgaac aaagtggtcg acgtcgtcgg tccctccgat
     2881 ttctatcgcg atgcccatgg gaagatctac gacctcatgg tccatctcta cgagaagaac
     2941 gatgccatcg atatcatcac ggtaagcagc ctcgcgaagg acaggggcct ccttgaaccc
     3001 ataggcggtg tgacctatct caacaccctt gtggacctca tgccgtccgc ggccaatgtc
     3061 gcctattacg cgaagatcgt aaaggagaag tccctcgtcc ggaccattat caacgtggcc
     3121 acggagatca tcgagaaagg tttcaatacc gagaccgaca tcgacacgta catcgacgag
     3181 gccgagaagc tcatattcca ggtctcggag aacaagctca aacccaccta tctgcccgtc
     3241 aaagacttcg tgatggacaa cataaagacc atcgagcgtc tctatgagaa gaagcagacg
     3301 gtgacggggg tccccacggg gttcatcgac ctcgacaggc tgacgagcgg cctgcagccc
     3361 tcggacctca tcatcgtcgc cgggaggccg agcatgggga agacctcctt ttccatgaac
     3421 atcgcccagc atgtgtccat gctgaaagag aaccctattc ccgtgggcat cttttccatg
     3481 gaaatgtcca aggagcagct cgtcacgagg ctcttgagtt ccgagtccga gatagagcat
     3541 tccaagcttc gcacgggcac gttctccagg gaggaatggc cgaaactggt gagggcggcg
     3601 gggaagctca gcgaggccca catgttcatc gacgattcgc cggcactgtc cgtcctcgaa
     3661 ctgagggcgc gggcccgccg cctcaagaag gagcacgacg tgggccttct tgtcgtcgat
     3721 tacctccaac tcatgcgggg gcgctccagc ggcggtgacc gaagggagca ggagatatcc
     3781 gagatatccc ggttcctcaa ggcgctggca aaggagatca acattcccgt tatcgccatt
     3841 tcccagctca acagggcgcc ggagacccgc gagaaggaga acaagcggcc gcgtcttgcc
     3901 gaccttcggg aatcgggggc aatcgagcag gatgccgacg tcatcctctt catctaccgc
     3961 gacgaggtgt ataataagga cagggacgac aacaaaggcg tcgccgaggt gatcatcgga
     4021 aaacagcgga acggtcccac agatacggtg aaactgagtt ttatcgataa atgcgcgacg
     4081 ttcaggaacg gctactatca agatgacgaa gggtgacggc atgcggtcca tgaacgttcc
     4141 caatatcctt tccattttcc gtttcttcgt cacattctac ttcatctatg ccgtctacca
     4201 gggacggatg cgcctcgccc tctacctctt tctcattcag ggcgtatcgg accttcttga
     4261 cggcttcatc gcgcgggtga cgggatcgaa gacc
//
LOCUS       DAOQTR010000130         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23129_length_4268_cov_3.631616, whole genome shotgun sequence.
ACCESSION   DAOQTR010000130 DAOQTR010000000
VERSION     DAOQTR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23129_length_4268_cov_3.631616"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1642
                     /locus_tag="PKN85_04305"
     CDS             50..1642
                     /locus_tag="PKN85_04305"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT43659.1"
                     /translation="MRQFLRNMSITQKMFVSPVVAAVLIAASGIVFHLYFPGSVNPVI
                     FAVMTAAVVIVLASAYVMAQLVRKPIGEISESFEGLSKGDLTKRLEEASSGEFGVMGR
                     NFNSFVERFSDALSGVADRSSQISFAVNTLDATASQMARGADVVLTEITSVATASEEM
                     SATATEITNNCIAAAKSSEIANNSAVTGENIIQEIIRSMGRIDERVKESALIIHKLGE
                     RSDQIGEIAGIINDIAEQTNLLALNAAIEAARAGEHGRGFAVVADEVRKLAERTTGAT
                     KQIGDTIQTMQNEMKNAVVSMEGGVKEVEIGNNETAKSGSALKEILNQINTLASQINQ
                     IAVASEEQSATTNEINNNIQKISKIMDAAAKNIAENADASSNLSNLSVELNKLANQFK
                     MQSRDQSQGSSVGREEVVLFVEKAAEYLKSAGDKKKVYNEMSDPHGRFVKGDLYVFVN
                     TLEGVTEAHGQNAKLVGKEMHELKDADGKFFIKEITEVAKTKGKGWVDYKWINPVTGK
                     ILEKTTYCQRVGDVYIGCGIYK"
     gene            complement(1819..>4268)
                     /locus_tag="PKN85_04310"
     CDS             complement(1819..>4268)
                     /locus_tag="PKN85_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNT43660.1"
                     /translation="LGQADVLRRMGQLGLRRGNIPEARASYEKALPIYRKVREPVGQA
                     DVYKGLGDIGYYGRNFSSALEMYDKALPYYVHAGEPLGQGNVHRATGDVYFYAGDYTK
                     AMEYYEKALSFYMKANSPMGQANTYRTMGETYLRVGKADHALAMFTSAMALYKKVNEP
                     IGQGDIYKTLGEIYLERGNRMGALDMFREALNYVTAAHSVVDQGHAYQGIGDVFLSAG
                     DLGRSLENYDTALELYRRMQDKESEAFVLIKKAGLAGRKGDVKEATGLFEAGLGKLEE
                     VRSQAMFPEMKKSYMEKVYGHYEDAAIFMLEANENEKAFRSIEAMKARVFLDQLAEAR
                     VDLEKGIDPAVKTERSTLTNELTIAGRKLGEESRKDAPDKEVVEKLKREFSLIQERLD
                     ALKRDIRYKNPLYASVQYPEPVAVKFLQEKVLKEGEVLAEYFLSKKGVYCLVVSRKDF
                     NVVKLSVNHEDLLSKVKAFLTNIRGYQQKERFRDALAEDLYAILVKPLETFLDGKTLI
                     VAPQGILAYLPFEALMTEEDGKKVFMIEKYPVTYVQSGTVLSVLRSQYEREGSAGGFV
                     GFGDPVYDYDEYLSETPEARDKKAEKKNGAVSHSAGDEPPAVSPGAAFTRNSYLRAGG
                     TLTRLQGSGEEIEGIRKIYEEQGGPARSFLRIEAREENARSPEMGRYAFIHFSTHGIL
                     EPGFQAIALSQIPGDREDGFLTLGEIMNSRFNARLVVLSACETGLGELSYSEGVTGLT
                     RAVMYAGSAAAVVSLWSVADEGTRDLMIRFYDGLVKKSISKVEALRAAKIELLKDTKR
                     NAFTHPFFWSAFVMYGE"
BASE COUNT          894 a         1221 c         1250 g          903 t
ORIGIN      
        1 attcttcgat aaagatgcaa gggagaataa aaaaagggag ggtcccgtcg tgagacaatt
       61 tttgcgcaac atgagcatta ctcaaaagat gtttgtttca cccgttgtgg cggctgttct
      121 gatcgccgcc tccggcattg tctttcatct ctattttccc ggttcagtga atcctgtcat
      181 tttcgctgtt atgaccgccg cggtggtgat cgtcctggcg tcggcatacg tcatggccca
      241 actcgtgcgt aaacctatcg gtgagatctc ggagtctttc gagggtctct cgaagggcga
      301 cctcacaaaa agacttgagg aggcgtcgag cggagaattc ggcgtcatgg gcaggaactt
      361 caacagcttt gtggagcgtt tcagcgatgc tctctccggg gtcgcggaca ggagcagcca
      421 gatatccttc gccgtcaaca ccctcgacgc gacggcgagc cagatggcac gtggcgccga
      481 tgttgtcctg acggagatca cctccgtcgc gacagccagc gaggagatgt cggcaacggc
      541 gacggagata acgaacaact gcatcgccgc ggcgaagagt tccgagatag cgaacaattc
      601 ggccgtgacg ggcgagaaca tcatccagga gatcatccgg agcatgggcc gtatcgacga
      661 gcgcgtcaag gagtcggccc ttatcatcca caaactgggg gagcgctccg accagatcgg
      721 cgagatcgcc gggatcatca acgacatcgc ggagcagacg aaccttctcg ctctcaatgc
      781 cgccatcgag gcggcccggg ccggtgagca cgggcggggg ttcgccgtcg tcgccgacga
      841 ggtccgcaag ctggcggaaa ggacgacagg cgcgacgaag cagatcgggg atacgataca
      901 gacgatgcag aacgagatga agaacgccgt cgtttccatg gaaggcgggg tcaaagaggt
      961 cgagataggc aacaacgaaa cggcaaaatc gggcagcgcc ctcaaggaga tcctgaacca
     1021 gatcaatacg ctggcctcgc agataaacca gatagcggtg gcctcggaag agcagagcgc
     1081 gacaacgaac gagatcaaca acaacatcca gaagatatcg aagatcatgg acgcggcggc
     1141 gaagaacatt gcagagaacg ccgacgcctc atcgaacctg tccaacctgt cggtggaact
     1201 gaacaagctt gccaaccagt tcaagatgca gagccgcgat cagtcgcagg gaagctccgt
     1261 cggcagggaa gaggtcgtgc tctttgtcga gaaggcggcg gagtatctca agagcgccgg
     1321 cgacaagaag aaggtctaca atgagatgag cgatccccat ggcaggttcg tcaagggtga
     1381 cctgtacgtg ttcgtgaaca ccctggaggg tgtcaccgag gcccacgggc agaacgccaa
     1441 gctcgtcggc aaggagatgc atgaattgaa ggacgcggac ggcaagttct tcataaagga
     1501 gatcaccgag gtggcgaaga cgaaaggcaa gggctgggtg gactacaagt ggataaaccc
     1561 cgtgacgggc aagatcctgg aaaagaccac ctactgccag cgagtaggcg acgtctacat
     1621 cgggtgcgga atctataagt aaagacggct tgagtcgctt gaatagcgtg agtgttagaa
     1681 gcctctttta acgagagcct tgtctttggc ccttaacaac aggttatcgg agctgaagag
     1741 cctcgtccgg aaaagctatt ggctttaact ctcaagctat tcaagccatt caagccattc
     1801 aagccattca agccgttttc actctccata catcacgaac gccgaccaga agaaggggtg
     1861 ggtgaaggcg ttccttttcg tgtccttgag gagctcgatc ttcgcggccc ttagggcttc
     1921 gacctttgat atgctctttt ttaccagacc gtcatagaag cggatcatca ggtctctcgt
     1981 gccttcgtcg gcgacgctcc agaggcttac gaccgccgcg gcgctgccgg cgtacatcac
     2041 ggctctcgtg agccccgtga cgccctcgga atagctcaac tcgccaaggc cggtctcaca
     2101 ggccgacagg accacgagac gggcgttgaa acggctgttc atgatctctc ccaaagtcag
     2161 gaacccgtct tccctgtccc ccggtatctg gctcaacgct atggcctgga acccgggttc
     2221 gaggatgccg tgggtggaga agtgaatgaa ggcataccgg cccatctcgg gagacctggc
     2281 gttctcctcc cgggcctcga tcctcaggaa tgaacgggca gggccgccct gttcttcgta
     2341 tatcttccgg atcccttcta tctcctcccc gctcccctga agccgtgtca gcgtgcctcc
     2401 ggcacggaga tagctgttcc tcgtgaatgc cgcgcccggg ctcacggcgg ggggttcatc
     2461 tccggcgctg tggctcacgg caccgttctt tttctccgct ttcttgtccc gcgcttcagg
     2521 ggtctccgac aggtactcat cgtagtcgta aacggggtct ccgaaaccga cgaagccgcc
     2581 cgctgatccc tcgcgttcgt actggctgcg caataccgag aggaccgttc ccgactggac
     2641 ataagtgacg ggatacttct cgatcatgaa taccttcttc ccatcttcct cagtcataag
     2701 ggcctcaaag gggagataag caaggatccc ctggggcgcc acgatgagcg tcttgccgtc
     2761 gaggaaggtc tcgagaggtt tgacgaggat ggcatacagg tcctctgcaa gggcatcgcg
     2821 gaaacgctct ttctgctgat aacccctgat attggtgagg aacgccttta ctttggacag
     2881 gaggtcttcg tggttcacgg agagcttcac cacgttgaaa tccttcctgc tcacgacaag
     2941 gcagtagacc cccttcttcg agaggaagta ctcggcgagg acttcgcctt ccttaaggac
     3001 cttttcctgg aggaatttta ccgcgactgg ctccgggtac tggaccgagg catacagggg
     3061 gttcttgtac cggatgtccc tcttcagggc atcgagtctt tcctggatga gagagaactc
     3121 cctcttcagt ttctccacga cctccttgtc gggtgcgtcc tttcgggatt cctccccgag
     3181 tttcctgccc gcgatcgtca gttcgttcgt gagggtgctg cgctccgtct ttacggccgg
     3241 gtcgatgcct ttctcgaggt cgactcgcgc ctccgcgagc tggtcgagga atacgcgggc
     3301 cttcatcgct tcgatggagc ggaaggcctt ctcgttctcg ttggcctcaa gcatgaagat
     3361 ggccgcgtcc tcgtaatggc catagacctt ttccatgtaa ctcttcttca tttcggggaa
     3421 catggcctga gagcgaacct cttcgagctt cccgaggccg gcctcgaaaa ggcccgtcgc
     3481 ctccttcacg tcccccttgc ggcctgcgag accggccttc ttgataagga caaaggcttc
     3541 cgattccttg tcctgcatcc ggcggtaaag ctcaagggcc gtgtcatagt tctcgagaga
     3601 cctcccgagg tcgcccgccg acagaaagac gtcgccgatg ccttggtacg cgtgtccctg
     3661 gtccacgacg gaatgggcgg ccgtgacgta gttcagggcc tcccggaaca tgtcgagagc
     3721 gcccatcctg ttgcccctct cgaggtagat ctcgccgagg gtcttgtata tatcgccctg
     3781 cccgatgggt tcgttgacct tcttgtagag ggccattgcg gacgtgaaca tggcaagagc
     3841 gtggtcggcc ttgccgactc tcagataggt ctcgcccatg gtgcggtagg tgtttgcctg
     3901 gcccatcggg gaatttgcct tcatatagaa ggagagggcc ttctcgtagt actccatggc
     3961 cttcgtataa tcaccggcgt aaaagtagac gtctcccgtg gcgcgatgga cattgccctg
     4021 ccccaggggt tcgcccgcat ggacatagta aggcagggcc ttatcgtaca tctcgagggc
     4081 gcttgagaag ttcctgccgt aatagcctat gtcgccgagc cccttgtaca cgtctgcctg
     4141 ccccacgggt tccctgacct tcctgtatat gggaagggct ttttcgtatg atgcgcgggc
     4201 ctctggtatg ttgcccctgc gcagaccgag ctggcccatc cttcggagga catcggcctg
     4261 ccccaaag
//
LOCUS       DAOQTR010000131         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23132_length_4268_cov_3.271778, whole genome shotgun sequence.
ACCESSION   DAOQTR010000131 DAOQTR010000000
VERSION     DAOQTR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23132_length_4268_cov_3.271778"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PKN85_04315"
     CDS             <1..241
                     /locus_tag="PKN85_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43661.1"
                     /translation="IMFENLTPLFPKEQMRLERLLFGFRTMWGVELQELSGKDCANRL
                     EDLREKGLVEVRGVRVVPTLRGYLFADRLPLMVSE"
     gene            577..1233
                     /locus_tag="PKN85_04320"
     CDS             577..1233
                     /locus_tag="PKN85_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT43662.1"
                     /translation="MYTGGLIGMILSAGPMAKVVMVILFFFSIVAWTIIFMKYRQYKR
                     TEAEGDRFFRAVKEADSFKKLISVYRDSQDNAFYRLVLACYKEVTSRQKDNPGRIRRE
                     DIPPIEDMLRIAISDESVKLERRLSFLATTANTAPFIGLFGTVWGIMDSFREIGVRGT
                     TSLAVVAPGISEALIATALGLATAIPAVLAYNYFLGRLKRILSRMENAAIYLVNILEK
                     "
     gene            1230..1643
                     /locus_tag="PKN85_04325"
     CDS             1230..1643
                     /locus_tag="PKN85_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HNT43663.1"
                     /translation="MKARGDSKGPLSEINVIPLVDVMLVLLIVFMITAPMMQHGLGID
                     IPNVTAKALPAKDEPQILNITKDQRLILNEKKIEAKDLKAAIQLLFASKDKKEIFLRA
                     DKDVPYGFVVRCMGTIREAGVDKVNIVTKPLDRNE"
     gene            1636..2415
                     /locus_tag="PKN85_04330"
     CDS             1636..2415
                     /locus_tag="PKN85_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNT43664.1"
                     /translation="MSEEAWTKMLVVSLALHIIVLGVFSIPIKFPSRKIDLSSAYSVN
                     LVGSTGSLGGGSAGPKIAAQSKPEPKPDKPAPVVKEKKAPPKKPQPIRKEDDAVSLSK
                     KKVPPKTKATKEEVDRLAERIRNIRKKTDYIDVAKAGSGGSGRGAGAGPGLPGSGGGS
                     GAPLDPAMQKYLLDVYEKIKNAWNVPGTARRELETIVTIKVRKDGRITDINIEKRSGN
                     RVYDESVLRVLRAVEPLPTIPQSLNTDSLEIGFRFVPGGIS"
     gene            2412..3209
                     /locus_tag="PKN85_04335"
     CDS             2412..3209
                     /locus_tag="PKN85_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNT43665.1"
                     /translation="MTALQSILMGAVQGITEFLPISSSAHLIVIPWFFNITQGNINPL
                     TYDVMLHFGSLFAVLFIYGKRFFRVVIEGLVDFRDGRGTDSTLFKMVIATIPAVVAGF
                     LGKDIIETYLRAPFVAAFMLALVSILMIVSERINVDRSELTMPIAIAIGVAQAIALVP
                     GTSRSGVTITMALLLGLKKSEAVDFSFMLSIPIILGVSLYELRHVEIQGDGAAIYALG
                     MLSAFVFGAASLKFLIGYLKKHTLDLFAYYRIALALLILVFSLIPRV"
     gene            3235..3402
                     /locus_tag="PKN85_04340"
     CDS             3235..3402
                     /locus_tag="PKN85_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43666.1"
                     /translation="MNMEKDKKEKKAKTISDDFVLYDPENLPYMKLCVCDIIRELPEL
                     TPEDIARFKNK"
     gene            3461..4258
                     /locus_tag="PKN85_04345"
     CDS             3461..4258
                     /locus_tag="PKN85_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43667.1"
                     /translation="MASFRREVMEFYGRNRRTFPWREDITPYRVVVSEIMLQQTGVER
                     VREKYEPFVRRLPDFSTLAAAPLKDIMTIWQGLGYNRRALSLKRLAGVVMERHSGRLP
                     QDRGSLLELPGVGEATAGAIMAFAFNVPAVFIETNIRRVFIHCFFPDEANVSDRQIVP
                     LIEAALDRSNPRDWYFALMDYGSPLGRTGSNANRKSLHYSRQSHFTGSDRELRGEAIR
                     VLLVRGPMTVNDIAAAARRAARRLVPVLDTMVGDGLLVREKESYRIA"
BASE COUNT          947 a         1210 c         1183 g          928 t
ORIGIN      
        1 gatcatgttc gagaacctga cgccgctgtt ccccaaggag cagatgcgcc tggaacgcct
       61 ccttttcggt ttcaggacga tgtggggggt tgagctgcag gaattgtccg ggaaggattg
      121 tgccaaccgt ctggaagatc tccgagagaa gggtctggtg gaggtccgtg gcgtgcgtgt
      181 cgtcccgacc ctcagaggtt acctctttgc agacaggctt ccattaatgg tttccgagta
      241 gcagtttacc agaataattt ttttattgac aaagatttcg taatacatta tcataactca
      301 agctatgtta aataattcac atatctcaga cgggaatagc ttgaaatcag cggtatttac
      361 gattgcccgt aattcagtgt gtgaaactac tggaaatttt atggtcacaa cgtgcattat
      421 ttcacatagc atcacctaac gcgaaattca cctccacaag cttctcataa taacccccct
      481 tccccctggc acccaaccag ggggtttttc tttcctttac aaccattgag ttttgttgta
      541 atctctacac gcaaaatgga cgtttcaagc gcagacatgt acacgggcgg gctcatcggg
      601 atgatcctct cggcgggacc gatggccaag gtcgtgatgg tcattctttt cttcttttcc
      661 atcgtagcct ggaccatcat cttcatgaag taccgccagt acaagaggac ggaggccgag
      721 ggcgaccggt tcttcagggc cgttaaggag gccgactcct tcaagaagct tatctcggtg
      781 taccgggaca gccaggacaa cgcattctac cgcctcgtgc ttgcctgtta caaagaagtg
      841 acctcgagac agaaggacaa cccgggcagg atccgcaggg aggatatccc ccctatcgag
      901 gacatgctca ggatcgccat atccgatgag tcggtcaagc ttgaacggag gctttccttc
      961 cttgccacca cggcaaacac cgcgccgttc ataggtcttt tcggaaccgt ttggggtatc
     1021 atggattcat tccgtgaaat aggcgtcaga ggcacgacga gcctcgccgt cgtggcgccc
     1081 ggcataagcg aggcgctcat cgccacggcc ctgggcctgg caaccgccat acctgcagtt
     1141 ctcgcgtaca actactttct cggaaggctc aagaggatcc tctctcgcat ggagaatgcc
     1201 gcaatctacc tggtgaatat cctggagaaa tgaaagcacg gggcgattca aaggggccgc
     1261 tgtcagagat caacgtcatt cccctcgttg atgtcatgct ggttctcctc atcgtgttca
     1321 tgataacggc acccatgatg cagcacggtc tcggcatcga catccctaac gtgacggcga
     1381 aggcccttcc cgccaaggac gagccgcaga tactgaacat cacgaaggac cagaggctca
     1441 tcctcaacga gaagaagatc gaggccaagg acctcaaggc ggccatacag ctcctctttg
     1501 ccagcaagga caagaaggag atatttctcc gcgccgacaa ggatgtgccc tacgggttcg
     1561 ttgtgaggtg catggggacc atcagggaag cgggcgtgga caaggtgaac atagtaacga
     1621 aaccgctgga tagaaatgag tgaagaagcc tggaccaaaa tgctcgtcgt atcgctggcc
     1681 cttcacatca tcgtgcttgg tgtcttttcc attcccataa aattcccttc gaggaagatc
     1741 gatctgtctt ccgcgtattc cgtcaacctc gtgggcagca cgggtagcct tggtggcgga
     1801 tcggccgggc cgaagatcgc ggcgcaatcg aagcccgaac cgaagccgga caaacccgca
     1861 ccggtggtca aggaaaagaa ggccccaccc aagaagccgc agccaatccg taaagaggat
     1921 gacgctgttt ctctttccaa gaaaaaggtt cctccaaaga cgaaggcaac gaaggaagag
     1981 gttgaccggc tcgcggagcg gatacgcaat atcaggaaga aaactgacta catagacgtt
     2041 gccaaggccg gatcgggtgg ttccggcagg ggcgcgggcg ctggcccggg cttgcccggc
     2101 tccggcggcg ggtccggcgc tccccttgac cctgcgatgc agaagtacct tctcgatgtc
     2161 tacgagaaga taaagaacgc ctggaacgtc ccgggaacgg caagaaggga actggagacg
     2221 atcgttacga tcaaggtaag gaaggatggg cggatcaccg acatcaacat cgagaaacgc
     2281 tcgggcaaca gggtatatga cgagtccgtg cttcgcgtcc ttagggcggt ggagccgttg
     2341 cccaccatcc cgcaatccct caacaccgat tccctggaga tagggttccg gttcgtgccc
     2401 ggagggatat cgtgacggcc ctgcagagca tccttatggg cgccgtgcag ggtataacag
     2461 agttccttcc catcagcagt tccgcccacc ttatcgtcat cccctggttc ttcaatatca
     2521 cccagggcaa catcaacccg ctgacgtatg acgtcatgct ccacttcggg tcgctcttcg
     2581 cggttctctt catctacggc aagagattct tccgtgtcgt catcgagggc ctcgtcgatt
     2641 tccgggatgg ccggggcacc gattccacac tcttcaagat ggttatagcc actatacccg
     2701 cggtggttgc cggtttcctc gggaaggaca tcatagagac ctacctcagg gcgccgttcg
     2761 tagccgcctt catgcttgcc ctcgtgtcca tcctcatgat cgtgtccgag aggatcaatg
     2821 tcgacaggag cgagttgacc atgcccatcg ccatcgcgat aggtgtcgcc caggccatcg
     2881 cccttgtgcc cgggacctcg cgaagcggcg ttaccatcac catggccctg cttcttggtc
     2941 tcaagaagag cgaggccgtc gatttctcct tcatgctgtc catacccatc atccttggag
     3001 tgtcgctcta tgagctgcgg cacgttgaga tccaggggga cggcgcggcg atctatgccc
     3061 tgggcatgct ttcggccttt gtcttcggcg cggcgagcct caagtttctc atcgggtacc
     3121 tgaagaaaca taccctcgac ctgtttgcct actaccgcat cgcgcttgcc ctgttgatcc
     3181 ttgttttctc cctcattccc cgcgtttgac atcgggatgt gcccacttta cactatgaac
     3241 atggaaaagg acaagaaaga aaagaaggca aagaccatca gcgatgattt tgtcctctat
     3301 gatcccgaaa acctccctta catgaaactc tgcgtgtgcg acatcattcg ggagctgccc
     3361 gagttgaccc ctgaagacat tgccaggttc aaaaacaaat aggacatttc cggatcacag
     3421 gctctcaact cccgtcgaac ctcgcgggac agtgccggac atggcgtcgt tccggcggga
     3481 ggtcatggag ttctatgggc gcaacaggcg aaccttcccc tggagggagg atatcacacc
     3541 ttacagggtg gtcgtctcgg agatcatgct tcaacagaca ggggtggaac gggtgaggga
     3601 aaagtacgag ccttttgtcc ggcgcctgcc ggatttctcg accctcgcgg ccgcacctct
     3661 gaaggacata atgacaatct ggcagggtct cggttataac cgcagggcgc tttcgctcaa
     3721 gcgccttgcc ggagtggtga tggagagaca ttcgggtcgg cttcctcaag accggggatc
     3781 tctgctcgaa ttacccggtg tcggcgaggc cacggcgggg gccataatgg ccttcgcttt
     3841 caatgtgccc gcggtgttca tcgaaacgaa tatccggcgt gtcttcatcc attgtttttt
     3901 ccctgatgag gcgaatgtct cggaccggca gatcgtgcct ctcattgaag ccgccctcga
     3961 ccggtcgaat cctcgggact ggtacttcgc attgatggat tacgggtctc cccttggcag
     4021 gaccgggtcc aacgccaaca ggaaaagctt gcactactcg agacagagcc atttcaccgg
     4081 ttccgacagg gaattaaggg gagaggcgat cagggtcctc ctcgtacgcg gtcccatgac
     4141 ggtcaacgac attgccgccg ccgcgcgccg cgccgcgcgc cggctggtgc cggtgctcga
     4201 caccatggtt ggggacggtc tccttgtccg ggaaaaggag agctaccgga tagcctgagc
     4261 catggccg
//
LOCUS       DAOQTR010000132         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23145_length_4265_cov_4.262233, whole genome shotgun sequence.
ACCESSION   DAOQTR010000132 DAOQTR010000000
VERSION     DAOQTR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23145_length_4265_cov_4.262233"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKN85_04350"
     CDS             complement(<1..310)
                     /locus_tag="PKN85_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43668.1"
                     /translation="MDIATLLGLIVGIGSVLAAFLMEGGHISALIQVPAMILVVFGTF
                     GAATVTTSVKQLINLPNLMKVAFSEKKMDSRQLIDLLFDLSQKARKNGLLSLEKDIDT
                     I"
     gene            complement(317..889)
                     /locus_tag="PKN85_04355"
     CDS             complement(317..889)
                     /locus_tag="PKN85_04355"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT43669.1"
                     /translation="MSGESERREFFRITDRIPLEFRPISRDEFERLEGIIRYKSTQVV
                     DRLNEVHFLECRGEPGEETDQMHAYMETINRKLDLILEILGGSSADSGYTAVKTDVNI
                     SGAGVQFVCEARLKEGDLVELKIIIPVFPYPRITCLCEVVRADRSDDAGSLRCALKFM
                     VINENDQDVLVNYIFLKERQYLRQKKETTS"
     gene            complement(879..2354)
                     /locus_tag="PKN85_04360"
     CDS             complement(879..2354)
                     /locus_tag="PKN85_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43670.1"
                     /translation="MIEGTKILVVDDNPEVINILGDFLGLNGCEIYKATCGREALEML
                     EKKDPEIVILDVQLPDLNGITLIDTIKVSKPTTAVIMATGYYDPNFVIDAMKKGASDF
                     LLKPFELDKLMLVMMRVLRERKLLIENESMMQNLNDKKKIEHLNRELQKKIKELTTMY
                     HISNRFNSINIFEDVYEKMVQIVGETLDIRSCGYYVPDGDTNELILYAGHTKNGEPPG
                     CDWQRISIPEDLSGPARRMRKHVVKDNRIYFPLVIQGEPIGFIMTEAKMNGRGQRSLE
                     NDALLLRLIAEKASTQIENRMLYESLFENILHTLKSLIIAITKRDLYTEGHCKRVTGM
                     SLALGERLGVSDYEKDVIKVVCPVHDLGKIGIPDRILLKPERLSDEEYSIMQSHSVYG
                     EEIMSRFEILAKEARVIRHHHERYDGKGYPDRLAGEDIPMCSRIIAVCDAYDAMVTDR
                     PYRKAMGADEIVAEIARCSGSQFDPEIVKAFVDLVRDGYEW"
     gene            complement(2395..2973)
                     /locus_tag="PKN85_04365"
     CDS             complement(2395..2973)
                     /locus_tag="PKN85_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43671.1"
                     /translation="MKNDDPFRILIVDDNKELREILEEYLKGEGHDAQGAANGREALE
                     RHNQEPFDLIITDLNMPEVPGMELIRTIRKENRDTEFVIITGYASMDSAVEAVRIGAF
                     DYIVKPFRMEELQVVVKNARDKITLKKLNAKLVQTLQSFYDEMERYRGKKPEEREGRD
                     EPANGKRASDTEQIVAEIRNLEKLRKGRLLIE"
     gene            complement(2963..3169)
                     /locus_tag="PKN85_04370"
     CDS             complement(2963..3169)
                     /locus_tag="PKN85_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43672.1"
                     /translation="MTISDYQISSVIRTYMKNMKVRMNRAGNEHEVDSREDEVNISRE
                     GMKKMMYDRIGEHVTEKLKRHDEK"
     gene            complement(3226..3555)
                     /locus_tag="PKN85_04375"
     CDS             complement(3226..3555)
                     /locus_tag="PKN85_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43673.1"
                     /translation="MKTRDERDLVYFEGMTLAALDQEDGAAFIQCLLMREKVCERMTA
                     SSAGLDADTAERLRANETRVIERLEEERSKLLKEIERYSEARRALRSYSPKFPLPPAP
                     AFFSFKK"
     gene            complement(3552..>4265)
                     /locus_tag="PKN85_04380"
     CDS             complement(3552..>4265)
                     /locus_tag="PKN85_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43674.1"
                     /translation="GEDDGDEGALYPSSADGLQSPTSFAVKFPGFTVRVINVQTGDAV
                     SPSSETGIESVAERMVDLSRAVTDLVNLQDIDPESGSDTTTIQTLLKETVARMVRDSF
                     ISASGQPNLGTFPVDAGAFGIGIDEKGGLRIDAVVLKEALTGHRDEVVRFVHDFAGSI
                     HDRITYGFNPIACVCRGGQENPFLSRAESKESLTDDEQSSRADFEKRLNELQMLLKSS
                     YELKDSFMQRKPARQGRPV"
BASE COUNT          820 a         1269 c         1071 g         1105 t
ORIGIN      
        1 ttatggtgtc gatatccttc tcgaggctca ggagcccgtt cttcctggcc ttttgggaca
       61 ggtcgaagag aaggtcgatg agttgccggg agtccatctt cttctcggag aatgccacct
      121 tcatgaggtt gggaagattg atgagctgct tgacagaggt ggtcaccgtt gccgctccga
      181 aggtgccgaa cacgacgagt atcatggccg gtacctggat cagcgcggag atgtgtcctc
      241 cttccatgag gaaggcggca agaactgaac cgattccgac tatgagtccc aggagggttg
      301 caatatccat agctcatcaa ctcgtcgtct cttttttttg acgcagatac tggcgttcct
      361 tcagaaaaat gtaattgacc aggacatcct ggtcgttctc gtttataacc atgaatttca
      421 gcgcacacct gagggaaccc gcgtcatcgc tcctgtccgc cctcaccacc tcgcacaaac
      481 aggtgatcct ggggtagggg aacacgggga tgatgatctt gagttccacc agatcaccct
      541 ccttcaggcg ggcctcgcag acaaactgga cgccggcgcc gcttatgttc acatcggtct
      601 tcaccgcggt gtagccgcta tcggcggaag acccgcccag gatctccagg atcagatcga
      661 gcttcctgtt gatcgtctcc atgtacgcat gcatctggtc cgtctcttcc ccgggctctc
      721 cacggcattc caggaagtgg acctcgttca gtctgtcgac cacctgcgtg gacttgtagc
      781 ggatgatgcc ttcaagcctt tcgaactcat cacggctgat cggacggaat tcgaggggga
      841 tcctgtcagt gatcctgaag aactcccttc tttcggactc accactcata gccatcccgg
      901 acgagatcga cgaaggcctt cactatctcg ggatcgaact ggctgccgct gcatcgcgcg
      961 atctccgcca cgatctcgtc cgcccccatc gccttgcggt atggacggtc agtgaccatg
     1021 gcatcgtaag catcgcacac cgctattatg cgtgaacaca tggggatgtc ctcgcccgcg
     1081 agacgatcgg gatacccctt gccgtcataa cgctcatggt gatgcctgat gaccctggcc
     1141 tccttcgcca ggatctcaaa acggctcatg atctcttccc cgtacacgga atggctctgc
     1201 atgatggaat attcctcgtc ggagagcctc tccggcttga gcagaatgcg gtcggggatc
     1261 cctatcttcc ccagatcgtg gacagggcaa acgaccttta taacgtcctt ctcgtagtcg
     1321 ctgacaccga ggcgctcgcc tagcgcgagg ctcatgcccg tgaccctctt gcaatgccct
     1381 tccgtgtaaa ggtcgcgctt cgtgatggcg atgatgagcg acttcagagt gtggaggatg
     1441 ttctcaaaga ggctctcgta gagcatcctg ttctctatct gtgtcgacgc cttctcggcg
     1501 atgagacgaa ggaggagggc gtcgttctcg agggacctct gtcccctgcc gttcatcttc
     1561 gcctctgtca tgatgaagcc tatgggctcc ccctggatga caagcggaaa atatatcctg
     1621 ttgtccttca cgacatgctt tcgcattcgc cgcgccggcc cgcttaagtc ttcgggtatg
     1681 ctgatgcgct gccagtcaca tcccggcggc tcgccgttct tcgtgtgtcc cgcgtagagt
     1741 atcagttcgt tcgtatcacc gtcgggaaca tagtacccgc aggagcggat atcgagcgtc
     1801 tcgcccacga tctggaccat cttctcgtag acgtcctcga agatgttgat ggaattaaac
     1861 ctgttcgaga tgtggtacat cgtggtcagc tcttttatct ttttctgcag ttccctgttg
     1921 aggtgctcga tcttcttctt gtcgttcagg ttctgcatca tgctctcgtt ctcgatgagg
     1981 agtttcctct ccctgaggac cctcatcatg acaagcatga gcttgtcgag ttcgaagggc
     2041 ttgagaagaa agtcggaggc gccctttttc atggcgtcta tgacaaaatt ggggtcgtag
     2101 tagcccgtcg ccatgataac ggcggtggtg ggcttcgaga cctttatggt atcaatgagt
     2161 gttatgccgt tgagatcggg aagctggacg tcgagtatga ctatctccgg gtccttcttc
     2221 tccagcatct ccagggcttc cctgccgcag gtcgccttgt atatctcgca gccgttgaga
     2281 ccaagaaagt ctcccaggat gttgatgacc tcgggattgt catcgacaac gagtatcttc
     2341 gttccctcta tcatcgctca tccctcacgc cttctggcag gacaagacat tctgtcactc
     2401 aatgagcagt ctcccctttc tcagtttttc caggttcctt atctcggcaa cgatctgttc
     2461 cgtatcgctt gccctcttgc cgttcgcggg ttcatccctt ccctcccgtt cctcgggctt
     2521 tttcccgcga taccgttcca tctcgtcgta gaagctctgg agggtctgga cgagcttcgc
     2581 gttcagcttc ttcagggtga tcttgtcccg ggcgttcttg acgaccacct ggagctcttc
     2641 catcctgaaa ggcttgacga tgtaatcgaa ggcgccgatc ctcaccgcct cgacggcgct
     2701 gtccatcgag gcatagcccg tgatgatgac gaactccgtg tcccggttct ctttccttat
     2761 ggtccgtatg agctccatgc cgggtacctc cggcatgttg aggtccgtga tgatgaggtc
     2821 gaagggctcc tggttgtgtc tctcgagggc ttccctgccg ttggccgccc cttgtgcgtc
     2881 gtggccctcc cctttcaggt actcctcaag gatctccctg agttccttgt tgtcgtcgac
     2941 gatgagaatg cggaagggat catcattttt catcatgtct ctttaatttc tcagtcacgt
     3001 gctcaccgat gcgatcgtac atcatcttct tcatcccttc ccgtgaaatg ttcacctcat
     3061 cctcgcggct gtccacttca tgttcgttac cagccctgtt catacggacc ttcatgttct
     3121 tcatgtaggt tcttatgaca ctgcttatct ggtaatccga gatggtcatt ctacttctcc
     3181 ctgttacttt atcggaccgg gggaaggatg acttaagcca tttttttatt tcttgaaaga
     3241 aaaaaacgcg ggggccggcg gcaggggaaa tttcgggctg taggacctca gagcccgacg
     3301 agcctccgag tatcgttcta tctccttgag cagtttcgag cgttcctctt cgagcctctc
     3361 tatgaccctc gtttcgttgg cacggagcct ctccgccgtg tcggcatcaa gtcccgccga
     3421 cgaagcggtc atgcgctcac aaaccttttc acgcatgagc aggcactgaa taaatgcggc
     3481 gccgtcctcc tgatcgaggg ctgccagcgt catcccctcg aaatagacga ggtctctctc
     3541 gtcacgggtc ttcatacggg ccgcccctgc cttgccggct ttctctgcat gaaagagtcc
     3601 ttcaactcat acgaactctt cagaagcatc tgcaactcgt taagtctctt ctcgaaatcc
     3661 gccctggaac tctgttcatc gtcagtcagg ctctctttgc tctccgcacg actgaggaag
     3721 gggttctcct gtcctcccct gcaaacgcag gcaattggat taaaaccata ggtgattctg
     3781 tcatgaatag aacccgcgaa atcatggaca aaacggacaa cctcgtccct gtgccctgta
     3841 agagcctctt tcaaaaccac cgcgtcgatc ctcaggccgc ctttttcgtc gataccgatc
     3901 ccaaacgctc ctgcatcgac cggaaacgtg cccaaattcg gttggcccga agcactgata
     3961 aatgagtctc tcaccattct cgcgacagtc tctttcagga gggtctgtat cgtagtcgta
     4021 tcagatccgc tctcagggtc aatatcctgc aaattgacca gatcggtaac agccctggaa
     4081 agatcgacca tcctttctgc gacgctttca atccccgttt ccgaactcgg cgacaccgcg
     4141 tcacctgtct gcacattgat gaccctgacc gtgaatcccg ggaatttcac agcaaaggag
     4201 gtcggactct gcaatccatc cgcggacgaa gggtaaagag ccccctcatc gccatcatcc
     4261 tcccc
//
LOCUS       DAOQTR010000133         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23247_length_4253_cov_3.648642, whole genome shotgun sequence.
ACCESSION   DAOQTR010000133 DAOQTR010000000
VERSION     DAOQTR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23247_length_4253_cov_3.648642"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKN85_04385"
     CDS             <1..537
                     /locus_tag="PKN85_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT43675.1"
                     /translation="KEVLGVLRKARWRTEFAEVGGTDIRGCSACGLCVVKKDRQCALK
                     KDGFNEVFAKMLKADAIILGSPVYFGGVSSDMKALVERSGYVARANGNAFAGKIGAAV
                     VALRRGGAIHTFDTINHMFLMSRMIVPGSTYWNMGFGRDKGEVSSDKEGLANMRHLAA
                     AIDWLARAIKPHTARYPE"
     gene            649..2370
                     /locus_tag="PKN85_04390"
     CDS             649..2370
                     /locus_tag="PKN85_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43676.1"
                     /translation="MSLRDRVCETPEELMSSELFLTVLKRFVEDLKGKRSALLEIFGK
                     QIDEIGDRDILELGQVFRNLVKMPLETVARLMEGWDDLVPRRSRLQTFVEGLYNYWRE
                     FERFIICDSAGDRLDRRPYRTFASTIESLTHLVRQAYRDIEENITGMHPSVYRQVRAG
                     AEVAVIALPKDTGLPGGSYRKLRDVPMIRQILLYPPLLLNPPMNKRTGKFERIPQNPL
                     ELMEIAPDEWLCYPAKVGPLLVLVYVHEKFYELGLSLCNLFELADDEFLDRPVDAVYL
                     FGVPSGALDGLAPMPTVFCDDEQNGLLVAACPNEDQFGYFGYLKKMVLTLHNVKVMKL
                     GRMPYHGAMVRIVTKGDRSLTLLIIGDTGAGKSETLEAFRTMGEDRIEEMTIIADDMG
                     SLDIGQEGSVIGYGTEVGAFVRLDDLQPGYAFGQLDRSIIMNPSQVNSRIVLPVTTYE
                     NVMEGCSVDMVLYANNYEETDEEHPIIERLNTPEGALHVVREGTAMSKGTTTSVGLVH
                     TYFVNVFGAVQYKALHDDIARRFFRAFFEKGIFVGQLRTRLGIPGWERKGPEQAAQAL
                     LGMIADR"
     gene            complement(2554..3915)
                     /locus_tag="PKN85_04395"
     CDS             complement(2554..3915)
                     /locus_tag="PKN85_04395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT43677.1"
                     /translation="MGFSSFKLHPKIAASIKALGYQVPTPIQVQAIPPVLAGRDVMGL
                     AQTGTGKTAAFVLPILEHLLPGPRGRVRSLIIAPTRELAEQIHVSIGELGRNTHLKSC
                     TVYGGVGVNPQVSKLRSGVDIVVACPGRLLDHLTQKTIDLEDLEILVIDEADRMFDMG
                     FLPDIRRIIRQLPVKRQTLMFSATMAPSIRKLTDEVLSDPVTVKIGHTNPADTVSHAL
                     FPVEQHLKTRMLMELLRQTDTESILIFTRTKYRAKRIGQELQKAGYRAASLQGNLSQN
                     KRQEALDGFRDGSYQILVATDIAARGIDVSNISHVINYDMPDTTDAYTHRIGRTGRAA
                     KTGDAFTFVCQEDEPQVRAIERVLGARIERRTVKDFDYTTPAPERNVEFARPPRPQRR
                     DRAQVKPERAGTGKEDNKKPGTGSPGSARAGLCASGPKPAGPSTQRHRRKRTGPSSKR
                     RGG"
BASE COUNT          822 a         1207 c         1373 g          851 t
ORIGIN      
        1 aaggaagtgc tgggtgtgct caggaaggcc cggtggagaa cggagtttgc ggaggtcggg
       61 ggcaccgaca tccggggctg cagcgcctgc ggcctgtgcg ttgtcaagaa ggaccggcaa
      121 tgcgccctca agaaggacgg cttcaatgag gtcttcgcga agatgctgaa agcggacgcc
      181 atcatcctcg gctcacccgt ttacttcgga ggggtctctt cggacatgaa ggcgctcgtg
      241 gaacgctccg gctacgtggc gcgcgcgaac gggaacgcct ttgccgggaa gataggggcc
      301 gccgtggtcg ccctgcgcag gggcggagcc atacacacct tcgataccat caatcacatg
      361 ttcctcatgt cacggatgat agtccccggc tcgacctact ggaacatggg cttcggccgg
      421 gacaaggggg aggtttcctc cgacaaggag gggctcgcca acatgcgcca tctggcggcg
      481 gcaatagact ggctcgcgcg ggctataaaa ccccatacgg cccggtaccc cgaatagccg
      541 cggcgcgtgc ccccgtcaca gtgccgggaa cccgaagaaa gacagaactg gagaacgagg
      601 actatggaag atcgggtcgc atttaaattt gtaggtagaa aggtcatcat gtccctccgg
      661 gacagggtgt gcgaaacccc ggaagagctg atgtcgagcg aattgtttct caccgtgctg
      721 aagcggtttg ttgaggacct gaagggaaaa cgatccgccc tgctggagat cttcggcaaa
      781 cagatcgacg agatagggga cagggatatt ctggaactgg gacaggtgtt tcggaacctc
      841 gtcaaaatgc cgctcgagac cgtggcgaga ctgatggagg ggtgggacga tctggtcccc
      901 agacggtccc gtttgcagac cttcgtggag ggcctgtata attactggag ggaattcgag
      961 cgcttcatca tctgtgattc cgccggggac cgcctggaca ggcgtccgta ccgcacgttc
     1021 gcttcaacga tcgagtcatt gacccatctg gtgcgacagg cttaccggga catagaggag
     1081 aacattaccg gtatgcatcc cagtgtctac cggcaggttc gggcgggagc ggaagtggcc
     1141 gtgatcgcgc tgccaaagga caccggattg cccggcggga gttatcggaa gctgcgcgac
     1201 gttcccatga tccgccagat cctgttgtac ccgccgctcc ttctgaatcc tccgatgaac
     1261 aagcggacag ggaaattcga gaggataccc cagaaccccc tggagctgat ggagatcgct
     1321 cccgacgagt ggctctgtta ccctgccaag gtcggtccgc tcctcgttct cgtgtatgtg
     1381 cacgagaagt tctacgagct tggcctgtcg ctgtgcaacc tgttcgagct ggccgacgac
     1441 gagttcctcg atcgccctgt cgacgcggtc taccttttcg gagtgccttc cggcgccctc
     1501 gacggcctcg cgcccatgcc gaccgtcttc tgcgacgatg aacagaacgg cctcctggta
     1561 gccgcctgtc cgaacgaaga ccagttcggg tacttcggat atctcaagaa gatggtgttg
     1621 acgctccaca acgtcaaggt catgaaactg gggagaatgc cctatcatgg tgccatggtc
     1681 aggatcgtga cgaaaggcga ccgttctttg acgctgctga tcatcggtga cacgggggcg
     1741 gggaaatcgg agacgcttga agcgttcagg accatgggag aagacaggat cgaggagatg
     1801 accattatcg ccgatgacat ggggtcgctg gacataggac aggaagggag cgtcatcggc
     1861 tacggtacgg aggtcggggc ctttgtcagg ctcgatgacc tccagccggg ctatgccttc
     1921 ggtcagctcg accgctcgat catcatgaac cccagccagg ttaactcacg gatcgtgctg
     1981 ccggtgacca cgtacgagaa cgtgatggaa ggctgttccg ttgacatggt gctctatgcg
     2041 aacaactacg aggagaccga cgaggagcac ccgatcatcg agaggcttaa tacccccgaa
     2101 ggcgcgctgc acgtggtccg ggaggggacc gccatgagca agggcaccac cacatcggtt
     2161 ggcctggtgc acacatattt cgtgaatgtc ttcggagcgg tccagtacaa ggcgcttcac
     2221 gacgacatcg ccaggaggtt cttccgggcc ttctttgaaa aagggatatt cgtggggcag
     2281 ttgcggacga ggctggggat ccccggctgg gaacgcaaag gaccggaaca ggccgcgcag
     2341 gcgctgctgg gaatgatcgc ggatagatga ggtccgggcc ttcccggacc gggtggtcgc
     2401 cggccggcgg gtcgtgcctg tcccacccga tggtccgccc gtcctggcgg gcatcacctt
     2461 tttacccccc actgcgttaa ggcggtagcc ggagatcatg cgaaggacag gaccgggagg
     2521 cacgaactcc atcccgttat gggaagggag gccttatcct ccccgacgct tggaagatgg
     2581 gcccgttctt tttctgcggt gcctctgtgt cgacggaccc gccggtttgg gtccggaggc
     2641 acagaggccg gcgcgcgccg acccggggga acccgtgccg ggttttttgt tgtcttcctt
     2701 tcctgtcccc gcccgttccg gcttcacctg cgccctgtcg cgtctttgcg gccgcggcgg
     2761 ccgcgcgaat tcaacgttgc gctcaggcgc gggtgtcgtg tagtcaaaat ccttcaccgt
     2821 gcgccgctcg atcctggcac cgagcacacg ttcgatggcc ctgacctggg gctcgtcttc
     2881 ctgacagacg aaggtgaatg cgtcccccgt cttagccgcg cggccggtgc ggccgatgcg
     2941 gtgggtgtat gcgtccgtgg tgtccggcat atcgtagttg atcacgtggg atatgttcga
     3001 tacatcgatg ccccgcgccg cgatatccgt cgccacgagg atctggtagg acccgtcacg
     3061 gaagccgtcg agagcctcct gacgcttgtt ctgtgaaagg ttcccctgca gtgaggcggc
     3121 cctgtatccg gccttctgca attcctggcc gatgcgtttt gcacggtact tggtgcgtgt
     3181 aaagataagg atggattccg tatcggtctg ccggagaagc tccatgagca tcctggtctt
     3241 caggtgctgc tccacgggga agagcgcgtg ggacaccgtg tcggccggat tcgtatggcc
     3301 gatctttacc gtgacggggt cggaaagcac ctcgtcggta agcttgcgga tgctcggggc
     3361 catggttgcg gagaacatga gcgtctgccg tttcacgggc agctgcctga tgatccgcct
     3421 gatgtcgggg aggaagccca tgtcgaacat ccggtccgcc tcatcgatga cgaggatctc
     3481 gaggtcctcg aggtcgatcg tcttttgtgt aaggtgatcg aggaggcggc cgggacaggc
     3541 cacgacaata tcgacgccgg aacggagctt gctgacctgc gggttaacgc ccacaccacc
     3601 gtagacggtg cagctcttca aatgggtgtt gcgccccagc tctccgatgg acacgtgtat
     3661 ctgttccgcc agttcccgtg tgggtgcgat gatgagcgag cgcacccttc cccgcgggcc
     3721 ggggaggaga tgctcgagta tgggcagcac gaaggcggcc gttttgcccg taccggtctg
     3781 tgcaaggccc atgacgtccc tgccggcgag gaccggcggg atcgcctgca cctgaatggg
     3841 ggtggggacc tgatagccga gcgcctttat actggcggct atttttggat gaagcttgaa
     3901 tgaactgaaa cccaagagca ccttctttca aataagatcg acagccaacg ggacaggaga
     3961 gcccgaaccg gccaggactg ttccggggag tcatcgggct caatgaaatt gtctgttatg
     4021 ggaacgtttc ggacagaaaa tcttcacgcg gccagggatc gttgcttcgc agcccggtga
     4081 acactataag atatagagcg gcccctgtca aataagtctt ccttcggacg ttttttctat
     4141 gaggagcagc ttcccggcac gggagagcct ctttatctcc ctctcccttt tcagggcatc
     4201 ggatctcgtc gggcagggct ccctgtagac gagtcgaacc ggccggcggc cac
//
LOCUS       DAOQTR010000134         4232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23434_length_4232_cov_4.050275, whole genome shotgun sequence.
ACCESSION   DAOQTR010000134 DAOQTR010000000
VERSION     DAOQTR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23434_length_4232_cov_4.050275"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PKN85_04400"
     CDS             complement(<1..1442)
                     /locus_tag="PKN85_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNT43678.1"
                     /translation="MNSVVVLIISLAVFFVGYRLYAGYIDRKVIKTDPKRATPAKMYM
                     DGVEFMPTSKNVLFGYQFKSIAGAGPIIGPIIAIQWGWLPALVWILLGTFFIGWVQDY
                     GSAIMAMRHEGASFGGLSHKLISPRARTILLSFVYFYLLLIAGAFGNVVVDTAIRLKP
                     APMAWLFLTVAGVVAGQMIYRWKRDIILTTVVTVVIAMVGIWVGTVAPSNQIIGESLS
                     NSRLLWALAAFVFCYFAAVMPIWKFALPVNYVAAYIVFIGLFVGIIGIFVLHPDFTLP
                     AVTSFSIPIGPIWPIMFVTIACGAISGWHSIVSSSGTARQLENELDARPVGAGVMFVE
                     MMLAAFALIIAGTIYASPADYGAAIKLGPGGVFAAGVAKFLNAVGLPTAIGRSYGSVM
                     MVVLAITIMQLVVRFMRVATSELLAGWHPVFRNAHVGTFIASVLGMVLVLTGWWQYLW
                     VLFGGANQLMASLALMLITAWLMSEGKSYS"
     gene            1608..2543
                     /locus_tag="PKN85_04405"
     CDS             1608..2543
                     /locus_tag="PKN85_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HNT43679.1"
                     /translation="MKVCFFGVGGVGGYYGALLTRYFDSTGTGRTYFIARGRHGAAIV
                     ERGLTLKKDGGGAEIRVTPFACSNTVAGLPVCDMIVVSVKGYDLDAATEQVAAITDDR
                     TIVLPLLNGADIYERMRRRLDKGCIFASCLYLGTHIESPGVIFQMGGSGQIAIGRDPL
                     YPDIYPEKLIELFRNAGILIEFHEDVNVEIWKKYIFIAPFALVTAAWSKTIGEVAADA
                     RLARLVRDIMKEIAAIAKALGIELPPDIVETSFLKAGQFPFETKTSFQRDVETKGRQS
                     EWDLFGGTVIRYADKLGVPAENTRATLEQLLKGLK"
     gene            complement(2782..3462)
                     /locus_tag="PKN85_04410"
     CDS             complement(2782..3462)
                     /locus_tag="PKN85_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNT43680.1"
                     /translation="MNITKEELKDVLLRHKQWLSLDEGGECADLSGIDLSEANLAGAN
                     LAGADLSRANLTRADLSQANLFDARLTGADLSGANLSRANLSRAYMARSDLARADLSE
                     ANLSDVNLVRANVSEADLVGATLTGINLSNTDLSNTDLSGATLADAILARADLTAAIL
                     ARADLSRAYLARADLFEADLTGADLSEANLTGANLCEADLTGADLSRAKLDNAEFAGA
                     QWIDGTKK"
     gene            3847..>4232
                     /locus_tag="PKN85_04415"
     CDS             3847..>4232
                     /locus_tag="PKN85_04415"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT43681.1"
                     /translation="MRTTENLSKVGFLHFLFLACLALIVFVPVRVCSADLPYTATIAE
                     GRRAVKEIMERTGASSVSVAFTDGDRLVWTETFGYADRASGTPPTKETMYCIGSTSKM
                     FATVAVMKLVDRGLIALDEPVVKHLKS"
BASE COUNT          900 a         1191 c         1304 g          837 t
ORIGIN      
        1 catgaatacg actttccctc cgacatgagc cacgcagtga tgagcatgag cgccagcgac
       61 gccatgagct ggttggcgcc tccgaagagc acccacaggt actgccacca cccggtgaga
      121 acaaggacca tgccgaggac actggcgatg aaggtgccca cgtgggcgtt gcggaaaaca
      181 ggatgccagc cggccagaag ctccgacgtg gcgaccctca tgaagcgcac gacgagctgc
      241 atgatcgtga tggccagcac caccatcatg acgctcccgt aagacctgcc gatagccgtc
      301 gggaggccca cggcgttgag gaatttcgcg acgccggccg cgaagacccc tccgggaccg
      361 agcttgatgg ccgccccgta atcggcgggg gaggcgtata tggtgccggc tatgatgagg
      421 gcgaaggctg ccagcatcat ctccacgaac atgacgcctg cgccgacggg ccgcgcgtcg
      481 agttcgttct caagctgccg cgcggtgccc gaactcgaca cgatgctgtg ccagcccgag
      541 atggccccgc aggctatggt aacgaacatg atgggccaga tggggccgat ggggatcgag
      601 aagctcgtaa cggcgggcag ggtgaagtcg gggtgcagga cgaagatccc gatgatcccg
      661 acgaaaaggc cgatgaagac gatgtaggcc gccacgtagt tgacggggag cgcgaacttc
      721 catatgggca tcaccgccgc gaaatagcag aaaacgaagg ccgcgagcgc ccagaggagg
      781 cggctgttcg aaagggactc gcctattatc tggttcgaag gcgccacggt gccgacccag
      841 atgccgacca tggcgatgac gaccgtgacg accgtggtga ggatgatgtc ccgtttccac
      901 ctgtagatca tctgtcccgc gacgacgccg gcaacggtca ggaagagcca tgccatgggc
      961 gccggtttca gccgtatcgc cgtgtcgacg acgacgttgc cgaaggcgcc ggcgatgagg
     1021 aggaggtaga aatagacgaa ggagaggagg atggtccgcg cgcgcgggct gatgagcttg
     1081 tggctcaagc ctccgaaaga ggccccctcg tgacgcatgg ccattatcgc gctcccgtag
     1141 tcctggaccc agccgatgaa gaaggttccc agcagtatcc agacgagcgc cgggagccag
     1201 ccccactgga tggcgatgat ggggccgatg atcggtcccg cgccggcgat cgatttgaac
     1261 tggtagccga agaggacgtt cttgctcgtg ggcatgaatt ccaccccgtc catgtacatc
     1321 ttggccggtg tggccctttt tggatccgtc ttgatgacct tcctgtcgat gtacccggca
     1381 tacaggcggt agccgacaaa gaacacggcc aaactgatta tcaggacaac gactgaattc
     1441 atacattcac ctccgtgttg cattggaatg gtgttatgcg ttcacatgtt gtcgctgcgt
     1501 catttttgtg atctgattta ccttattctc ctcccattaa aaccattttg caagaaagaa
     1561 attaaggtgt tatgctggtt catccctagt tcggaaagga atgtcccatg aaggtgtgtt
     1621 tctttggcgt cggaggagtg ggtgggtact acggagccct gctcacccgg tattttgact
     1681 cgaccggcac gggaagaacg tatttcatcg cacgggggcg gcacggggcc gccatcgtcg
     1741 agagggggct cactttgaag aaggacgggg gaggggcaga gatacgggtg acgccctttg
     1801 cctgttccaa caccgtcgcc ggattgcccg tatgtgacat gatagtcgtg tccgtgaagg
     1861 gctacgacct cgatgccgcc acggagcagg tcgccgcgat aacggacgac aggaccatcg
     1921 tattgcctct cctcaacggt gccgatatct acgagcggat gcggcggcgg ctggacaaag
     1981 gatgcatctt cgcctcctgc ctctatctcg gtactcatat agaatcgccc ggcgtcatct
     2041 tccagatggg tggaagcggc cagatagcga tcggcaggga ccctctgtac ccggacatct
     2101 atcccgaaaa gctgatcgag ctcttcagga acgccggcat ccttatcgaa ttccacgagg
     2161 acgtgaatgt ggagatatgg aagaagtaca tcttcatcgc ccccttcgcg ttggtgaccg
     2221 ccgcctggtc caaaacgatc ggtgaggtcg cggctgacgc cagacttgcc cgtctcgtca
     2281 gggacatcat gaaggagata gccgccatcg cgaaggccct cgggatagaa ctaccgcccg
     2341 acatcgtgga gacctccttc ctgaaggcgg gccagttccc cttcgagacg aaaacgtcct
     2401 tccagagaga cgtcgagacg aaaggcaggc aaagcgaatg ggacctcttc ggaggcacgg
     2461 tcatccgcta tgccgacaag ctcggcgtcc ccgcggagaa cacgagggcg accctggaac
     2521 aactactgaa gggtttaaag tgacacgcgt tatcggggcc tccgtggtcg ttgcctggca
     2581 ggtccccggg gttgcgcttc ccggaagctg tattataatt aagtataagg taagcttgcc
     2641 tcgccacttt ggtccgaagg gttccggccc ccaagccgga gtgaaggctc cccagccttc
     2701 acaacccttc gccaaggtgg tttctttagt gagtcttccg gtacttttgg cagtccgagg
     2761 ccttaaccga tcggaaggat tctatttctt tgttccatcg atccattgag cacccgcgaa
     2821 ctcggcatta tccagtttgg cacgggatag atccgcgccg gtgaggtcgg cctcgcagag
     2881 attcgcgccg gtgagattcg cctcactaag atccgcgccg gtgaggtcgg cctcgaagag
     2941 atccgcccgg gccaggtaag cacgggagag gtccgcgcga gccaggatgg cagccgtgag
     3001 gtccgcgcga gccaggatgg catcggcgag ggtggcgccg gacaggtctg tattggacag
     3061 gtctgtattg gacaggttga tgcctgtgag ggtggcgccg accagatcag cttcggagac
     3121 attggcccgg acgagattga cgtcggacag attcgcctcg gacagatccg cccgggcgag
     3181 atccgaacgg gccatgtatg cccgcgagag gttggcccgc gagaggttgg cacccgaaag
     3241 gtcggccccg gtcaggcggg catcaaagag attggcctgg gacaggtccg cgcgagtgag
     3301 gttggcacgg gatagatccg ccccggcaag attcgccccg gcaaggttgg cctcagagag
     3361 gtctatgccg gagagatcgg cgcattcccc gccctcgtcc agcgagagcc actgcttgtg
     3421 tctcagtagg acatctttca gttcttcttt tgtgatattc ataagactcc ctcatggatg
     3481 tgtacgaaca gagatactac taatccacga agcgtttatc aaataaattc ggctagacga
     3541 ttgttttccc gcacccccat tcgccccgaa acacctcgga gaaagcgagc attcttgaaa
     3601 tgctgagatg ccacctggcc cgtcaggtcg tacgctaacg accaattgcg agggatagcc
     3661 cggtctcccc tggccggttc cgtgttcccg atgaagaagt ctaagagacg cgccccattg
     3721 aacatggtct gaaggtttcg tgccttccgg cagggctttt ttcggattga cgagcctcta
     3781 cgcgtatcat atactcctca atacctgaca aatcctgaga attcataaag atagggggac
     3841 gagtagatga ggaccaccga aaacctgtca aaggttggtt ttctccattt cctgtttctt
     3901 gcgtgccttg cgctgatcgt attcgttcct gttcgggtgt gttccgcgga ccttccttac
     3961 acggccacga tcgccgaagg acggagggcg gtcaaagaaa tcatggagcg gaccggcgcg
     4021 tcttcggtct ccgtggcctt taccgatggt gacaggcttg tctggactga gacctttggc
     4081 tatgccgacc gggcgtcggg gacgccgcca acgaaggaaa caatgtattg catcggatca
     4141 acgagcaaga tgttcgccac cgttgccgtc atgaaactcg ttgaccgggg gctgatcgcg
     4201 cttgacgagc ccgtcgtgaa gcatctcaag tc
//
LOCUS       DAOQTR010000135         4226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23476_length_4226_cov_3.272596, whole genome shotgun sequence.
ACCESSION   DAOQTR010000135 DAOQTR010000000
VERSION     DAOQTR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23476_length_4226_cov_3.272596"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..454)
                     /locus_tag="PKN85_04420"
     CDS             complement(80..454)
                     /locus_tag="PKN85_04420"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNT43682.1"
                     /translation="MTLREVKEVLDAEVVVGHDQLDMEVERGACADLMSDLLVYCKTG
                     SLLLTGLANHQVIRTAEVLDAAAVVMVRGKRPRPETVKLAEELRIPLLTTKYILFETV
                     GRLYMKGMTGCIEKAGEERDFS"
     gene            769..1494
                     /locus_tag="PKN85_04425"
     CDS             769..1494
                     /locus_tag="PKN85_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT43683.1"
                     /translation="MIGLAGGAFGGLIGLGGGIIMIPLMAILAGLTQHQAHGTSLLAI
                     VFTALIGAVTYSLHGFADWRAALVLAICAMVTARFGALYAHSLPEKQLKRAFGWFLVV
                     VSLLLVFKGVILRSSYTLGPFLHYATLIGTGAFTGFLSGMMGVGGGGIMVPFLVIFIG
                     MPQHLAQGTSLLAMVPGSAIGAFTHHRLGNVAKHIALGLTLGAAIGGYIGASFAHLVP
                     EFYLKVIFALVGLWMGIRYIKTV"
     gene            complement(1664..2560)
                     /gene="ftcD"
                     /locus_tag="PKN85_04430"
     CDS             complement(1664..2560)
                     /gene="ftcD"
                     /locus_tag="PKN85_04430"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNT43684.1"
                     /translation="MKKIIETVPNFSEGRNPAKIRKILDCFTGVKGVKLLDHHKDKDH
                     NRLVVTAAGEPARVRKAVLDAVGVAVETIDLRRHRGEHPRMGAVDVIPFIPIGETSMD
                     EAVALSIDVAKEIWRSFRVPVFLYEESASHPDRRALPDIRRGEFEGMKEKIKDPRWKP
                     DFGDAAVHPTAGVVAVGARRPLVAFNVNLDTDSMAVATAIARAVRFQGGGLRYCRAIA
                     INLKERGIVQVSMNMTDYTRTPLYRALEMVRMEAKRYGVNVIGSEIVGLVPSDAMIES
                     AAYYMGLENFTTDQILENKLND"
     gene            2799..3110
                     /locus_tag="PKN85_04435"
     CDS             2799..3110
                     /locus_tag="PKN85_04435"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNT43685.1"
                     /translation="MRRSILVSTVMLFAVIALAACQTPAGRSAGDVVDDASITTQVKA
                     KLLEDDVTRGFAVSVQTFEGTVTLIGAVDTRAQIDRAGSIAASVKGVKAVDNRLKLKT
                     R"
     gene            complement(3144..>4226)
                     /locus_tag="PKN85_04440"
     CDS             complement(3144..>4226)
                     /locus_tag="PKN85_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT43686.1"
                     /translation="KAVAKDYGERPRLIYSRDAHEEAAWLVERIKEFYDQGMELSHQC
                     VLFRSSYITIPLQAELGRRNIPFAVYGGLKFYETAHVKDLIAHLKLFVNPKDELAWSR
                     VLLLVPGIGPKTAERMLGEMAAPETLSSIVENVIMPVGAARGRSSAGLTRLARLLEAA
                     ETEPEAVVRRVIDYYDPILREKFDLDWNLRKNDLDALAQISGRYGSVRDFLVDLAIEP
                     PERGVRDFHDTIWERPLILSTIHSAKGLEWPIVFLIGAVDGVLPSSYTTRDDEGLEEE
                     RRLLYVAVTRARERLYILAHHEGRNNGIHTFNRLSRFIEAQNVRACLDEGGSPRALTT
                     DDAPVAGSPEGMSKTDLLERLLHSLK"
BASE COUNT          792 a         1293 c         1227 g          914 t
ORIGIN      
        1 gcttcgaaaa ccgcaaccac ctcatcatca ccctcgcgag ggacatgaag aaggggaagg
       61 agtaagggcg ggcgagagat catgaaaaat cccgttcctc gccggccttt tcgatgcagc
      121 cggtcatccc cttcatgtag agcctgccga cggtctcgaa gaggatgtat ttcgtcgtca
      181 ggagcgggat gcgcaactct tccgccagtt tgaccgtctc cggccgggga cgcttgccgc
      241 ggaccatgac gacggcggcc gcatcgagga cctccgccgt acggatgacc tgatggttgg
      301 cgaggccggt cagcaggagg cttcccgtct tgcagtacac gagaaggtcg ctcatcagat
      361 cggcgcaggc acctctttcc acttccatat cgagctggtc gtgaccgacg acaacctccg
      421 cgtccagcac ctctttgacc tctcgcagcg tcatatcgtc cagactccat gaaaatgtca
      481 tgcactacac agaaatatca tatgttaaga ccgacagcgc ttcaactttt ctcttcgacg
      541 taaagacagt tcaagggttc aagggtaaaa gaagcatgaa aggcaggcga cgaacacctt
      601 cgaccggtgt acgaattgtg ttggcaagcg gtcttctttc gcggtctttc ccttgaaccc
      661 ccttgaaccc ttgaaccttc ttctccccat ggataaaccc gcccgctcgt ggtatattgg
      721 tttcttatgg ctgcggaatc gttctacaaa agtcagggaa agggtctttt gatcgggttg
      781 gcggggggag ccttcggcgg gcttatcggg cttggcggcg gcatcatcat gatcccgctc
      841 atggcgatac tcgccgggct cacccagcac caggcccatg gcacgagcct cctcgcgatc
      901 gtcttcaccg cactcatcgg cgccgtcacg tacagcctgc acggattcgc cgattggcgg
      961 gcggcgctcg tccttgccat ctgcgcaatg gtcaccgccc gcttcggtgc cctctatgcc
     1021 cactccctcc cggagaagca gctcaagcgc gccttcggct ggttccttgt cgtcgtgtcc
     1081 cttcttctcg tcttcaaagg agtcatcctc aggtcttcct atactctcgg tccctttctg
     1141 cactatgcca ccctgatcgg aaccggtgcc ttcaccgggt tcctctcggg gatgatgggc
     1201 gtcggaggag gcggcatcat ggtgcccttt ctcgtcatct tcatcggcat gccccagcac
     1261 ctcgcccagg gcacgtcgct cctcgcgatg gtaccgggaa gcgcaattgg agccttcaca
     1321 caccacaggc tcggcaacgt cgcgaagcat atcgccctcg gacttaccct cggtgccgcc
     1381 atcggcggct acatcggcgc gagcttcgcc cacctcgtac ccgagttcta cctcaaggtc
     1441 atcttcgccc tcgtcggcct ctggatgggg atcaggtaca taaagacggt ttaaaccgct
     1501 tgagccgctt gaacgtgaaa agacaataaa gaatattcct tgcattcaaa ccgccacaaa
     1561 ggccccttca gcgtgccggc gcaacaagga aaaagccttt aacgttcgag cggctcaagc
     1621 ggttgcaacg gtctttgcgg ttcaagcggc tgcaacggtc ttttcagtcg ttcagtttgt
     1681 tttccaggat ctggtcggtg gtgaaatttt cgaggcccat gtagtaggcg gcgctctcga
     1741 tcatggcgtc ggaggggacg aggcccacta tctcgctgcc gatgacgttc acgccgtacc
     1801 gctttgcttc catcctcacc atctcgagcg cacggtacag gggggtgcgg gtgtaatccg
     1861 tcatgttcat ggatacctgg actatgcccc gttccttgag gttgatggca atggcacggc
     1921 agtaacgcag gcccccaccc tggaagcgca ccgcgcgcgc tatggccgtg gccacggcca
     1981 tgctgtccgt gtcgaggttg acgttgaagg ccacgagcgg tcttctcgct cccacggcaa
     2041 ccacgcccgc cgtggggtgt actgccgcgt cgccgaaatc gggtttccat cgagggtctt
     2101 tgatcttctc cttcatcccc tcgaactctc ccctgcggat gtcggggagc gccctcctgt
     2161 cggggtggga ggccgattcc tcgtagagga acacgggcac ccggaaactc cgccagatct
     2221 ccttcgcgac atcgatggag agcgcgacgg cctcgtccat ggaggtctcg ccgatgggga
     2281 tgaagggtat cacatcgacc gcccccatcc ggggatgctc tcccctgtgc ctgcgaaggt
     2341 cgattgtctc cacggcaacg cccacggcgt cgagcacggc cttcctcaca cgcgccggct
     2401 cacccgcggc cgtcaccacc agtcggttgt ggtccttgtc cttgtgatgg tcgagaagct
     2461 tcaccccttt gacaccggtg aaacagtcga ggatcttcct gatctttgcc ggattgcgcc
     2521 cctcgctgaa gttgggaacc gtctcgatga tcttcttcat tgtgtgcgat cattgtaact
     2581 ccttgaaggg ctggagtaaa gaactagaat cgggtagctg cccggccgca ttcccttttt
     2641 ccctttccac gcttgtgaag gcaccaggaa ctgccgccag gcaatggtcc gcgggctgtt
     2701 catgttgact tccgagtggg agggcttagc tttccatgag aagccctccc tgccatgcca
     2761 ggccggcgag gcaaaacaaa ctgtaagagg ggaacattat gagaagatcc attcttgtat
     2821 cgacggtcat gctttttgcg gttattgccc tggccgcgtg tcagacacct gccggaagat
     2881 cggccggaga cgtagtcgat gatgcctcca taacaacgca ggtcaaagca aagcttctgg
     2941 aagacgacgt tacgaggggc ttcgcggtct ccgtccaaac ctttgaggga acggtaacgc
     3001 ttatcggggc cgtggatacc agggcacaga tagaccgtgc gggctcgata gccgccagcg
     3061 tcaaaggagt aaaagcggtg gataaccggc ttaaactgaa gaccaggtga gcgtcaggac
     3121 atcctttcaa ggttcccgtc gcgctatttc agggagtgca acagtctttc aagcaggtcc
     3181 gtcttgctca tgccttccgg cgaacctgca acgggggcat catccgtcgt gagagcccgc
     3241 gggctgccac cttcatcgag gcacgccctg acgttttgcg cctcgatgaa acgggagagc
     3301 ctgttgaagg tgtggatacc gttgttgcgg ccttcgtggt gggcaaggat gtaaagccgc
     3361 tccctggcgc gcgtcaccgc gacgtagaga agcctgcgct cctcttcgag gccttcgtcg
     3421 tcgcgggtcg tgtagcttga ggggaggacg ccgtccacgg cgccgatgag gaagacgatg
     3481 ggccactcca gccccttcgc ggaatggatc gtcgacagga tgagcggcct ttcccatatg
     3541 gtgtcgtgga agtccctgac cccccgctcg ggcggctcga tggcaagatc gacgaggaag
     3601 tcgcggacgg agccgtagcg gcccgatatc tgcgccaggg cgtcgaggtc gttcttcctc
     3661 agattccagt cgaggtcgaa tttttctctc aggatgggat cgtagtagtc gatcactctt
     3721 cgcaccacgg cctcgggctc tgtctcggcg gcttcaagga gacgtgcgag ccgggtaagc
     3781 cctgcggaag atctccccct ggccgcgccc accggcatga tgacgttttc cacgatggag
     3841 ctcagggtct ccggggcagc catctcccca agcattcttt cggcggtctt cgggcctatc
     3901 cccggcacga gcaggaggac gcggctccat gccagctcgt ccttcggatt gacgaagagc
     3961 ttgagatggg ctatgaggtc cttaacgtgt gccgtctcgt agaacttcag gcctccatag
     4021 acggcaaagg ggatgttgcg cctgccgagc tccgcctgca gcggtatcgt tatgtaggag
     4081 gaccggaaaa ggacgcactg atgggacagc tccatcccct ggtcgtagaa ctcctttatc
     4141 ctctccacca gccaggcggc ctcctcgtgg gcgtcccggg aatagatgag acggggcctc
     4201 tctccgtagt cttttgccac ggcctt
//
LOCUS       DAOQTR010000136         4209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23593_length_4209_cov_3.439336, whole genome shotgun sequence.
ACCESSION   DAOQTR010000136 DAOQTR010000000
VERSION     DAOQTR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23593_length_4209_cov_3.439336"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1233)
                     /gene="proB"
                     /locus_tag="PKN85_04445"
     CDS             complement(118..1233)
                     /gene="proB"
                     /locus_tag="PKN85_04445"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNT43687.1"
                     /translation="MKKSGAHMNRLVIKIGTSVLLDDEKKISTGRIRDIARQVRKVKE
                     KGIDVIIVSSGAIGCGMETLGLARKPKEMAKRQALASIGQVLLMKMYRENFEKKGMKV
                     SQILLTHEDIKNRTRCLNLTNTLDTLLKMDIVPVINENDALSFTEIKFGDNDNLSALI
                     AQIATADLLLILSDVEGLFDRDPNRYPDAKMIPVVHRIDEEIEGTATQSASEKSVGGM
                     VSKLEAAKKAGLYGIPTRVVLGGSKDVILKVVEGRQIGTLFLPGRKLARRKWWTAFAF
                     KPKGVIWVDQGAERAVVENGKSLLPSGVVRIDGHFTSGDCVEMKNGSGTVVARGIANY
                     SSSEMDRIKGLKSLDIEKELGYKYTEEMVHRDNMVVI"
     gene            complement(1237..2247)
                     /gene="obgE"
                     /locus_tag="PKN85_04450"
     CDS             complement(1237..2247)
                     /gene="obgE"
                     /locus_tag="PKN85_04450"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNT43688.1"
                     /translation="MKFIDEAIIYVESGKGGSGCVSFRREKFIPRGGPDGGDGGKGGD
                     VIIAGKKDLASLHDFRYKRNYKAENGKAGAGKNKTGREGRDVTIHVPLGTVVYDRDTS
                     ELLFQVLRDGETHAAALGGRGGRGNTRFVTSTHRAPLEFDAGEEGQRRWLHLDLKLLA
                     DVGLVGHPNAGKSTLISRLTQARPKVGDYPFTTLTPALGVLDDNEKTFVIADIPGIIE
                     GASKGKGLGLAFLKHIERTNALLIVLDLSSDDVKGDYRTLLEELGSYNEAMLQKRRLV
                     ILNKADLVTPDVAQRWRSYLKRKGEKAVVVSALTLAGVDELVSAISEGVEALRQNLNQ
                     AV"
     gene            complement(2247..2498)
                     /gene="rpmA"
                     /locus_tag="PKN85_04455"
     CDS             complement(2247..2498)
                     /gene="rpmA"
                     /locus_tag="PKN85_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNT43689.1"
                     /translation="MAHKKAGGSSRNGRDSQGQRLGVKIYGGQAIKAGSIIVRQHGTK
                     IHPGQNVGIGKDDTLFALIDGIVKFDMAGDRRRAHVVPV"
     gene            complement(2500..2817)
                     /gene="rplU"
                     /locus_tag="PKN85_04460"
     CDS             complement(2500..2817)
                     /gene="rplU"
                     /locus_tag="PKN85_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNT43690.1"
                     /translation="MYAIIENGGKQYKIEEGKKVRLEKFAGAEGDEVKIENVLAVNTG
                     ETTLIGSPYVSGAYINGKVVTQGRDKKVTVFKYKRRKDYKVKRGHRQPFTELLVEKII
                     VEA"
     gene            2963..3038
                     /locus_tag="PKN85_04465"
     tRNA            2963..3038
                     /locus_tag="PKN85_04465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2999..3001,aa:Pro,seq:ggg)
     gene            3067..3816
                     /gene="surE"
                     /locus_tag="PKN85_04470"
     CDS             3067..3816
                     /gene="surE"
                     /locus_tag="PKN85_04470"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNT43691.1"
                     /translation="MLILLTNDDGIHSKGIVTLGRYLSEKHDVYMIAPERERTCVAHA
                     VTLHKPLRVREVTRSAYATNGTPADCVLLGVHAVLPRRPDFVVSGINTGPNMGRDVNY
                     SGTVAAAREGAFLGIPSMAVSICAREGFLFDDAAGVACAVMERLTEGVFPQGTVVNIN
                     IPNLPREKLKGFSVTRLGKRIYNDIVHERVDPRGRKYYWIGGNGERYERRRGTDFYAI
                     EKGYVSITPLGLDITDAGSMKIYQKHFRRLL"
     gene            3813..>4209
                     /locus_tag="PKN85_04475"
     CDS             3813..>4209
                     /locus_tag="PKN85_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HNT43692.1"
                     /translation="MKTTLSIIKADIGSIGGHIMPSKKLIKRVIEFVESEAKGLVNDF
                     FVSHTGDDVAILFAHTNGVGFEKLHKLAWDAFLAGTQVAKKQGLYGAGQDLLKDSFSG
                     NVKGMGPAVAEMEFEERPNEPFVMFAADKT"
BASE COUNT          796 a         1276 c         1157 g          980 t
ORIGIN      
        1 aatggccgag ataggcgccg cccgacgcgg cggcgagcac cctgttcttc tcgtccgtgg
       61 atgcgtgcgc cagggtatct gatgcgcctt tcgccttctg ggcaagcttt tccggtctca
      121 tatcaccacc atgttgtccc tgtggaccat ttcttccgtg tatttatatc caagctcctt
      181 ctctatatca agacttttca ggccttttat cctgtccatc tcggaggagg agtagttcgc
      241 gatgccccgg gccacgaccg tgcccgaacc gttcttcatc tccacgcagt cgccactcgt
      301 gaaatggccg tcgatcctga ccacgcccga cggcaaaagg ctcttgccgt tctccaccac
      361 ggcgcgctcg gcgccctggt cgacccatat gacgcctttc ggtttgaagg cgaaggccgt
      421 ccaccacttg cggcgggcga gcttcctgcc ggggagaaag agcgtgccga tctgccggcc
      481 ctccacgacc ttcaggatca cgtccttgct gcctccgaga acgacacgcg tgggtatccc
      541 gtacagtccc gccttcttcg cggcttcgag cttgctcacc atgccgccga cgctcttctc
      601 gctcgccgac tgcgtagccg tcccctctat ctcctcgtcg atcctgtgaa cgacggggat
      661 catcttcgcg tcgggataac ggttgggatc acggtcgaag agcccttcca cgtcggagag
      721 tatgagaagc aggtcggccg tggcgatctg ggcgatgaga gccgacaggt tgtcgttgtc
      781 gccgaacttt atctccgtaa aggatagggc gtcgttctcg ttgatgacgg ggacgatgtc
      841 catcttgagg agggtatcaa gggtattcgt caggttgagg cagcgtgtcc tgttctttat
      901 gtcttcatgg gtgagcagta tctggctcac cttcatgccc ttcttctcga agttctcgcg
      961 gtacatcttc atgaggagca cctggccgat cgatgccagt gcctgccgct tcgccatctc
     1021 cttcggcttg cgggcgaggc cgagggtctc catgccgcag ccgatcgccc ccgaggagac
     1081 gatgatgaca tcgatgccct tctccttgac cttcctgacc tgccgggcga tgtccctgat
     1141 cctgccggtg gagatcttct tctcatcgtc gagaaggacg gaagttccta tcttgatgac
     1201 gagacggttc atatgcgcgc cgcttttctt catcctctag accgcctggt tgaggttctg
     1261 tctcaacgct tcaacgccct cggagatcgc cgaaaccaac tcgtcgaccc ccgcgagggt
     1321 aagagcgctg acgacgaccg ccttctctcc ctttctctta aggtacgacc tccaccgctg
     1381 agccacgtcc ggagtcacga ggtcggcctt gttgagtatc acgagccttc gcttctgaag
     1441 catcgcctcg ttgtagctcc ccaactcctc gaggagggtc ctgtagtctc ccttcacgtc
     1501 gtccgaggag aggtcgagaa cgatgagaag cgcgttcgtt ctctcgatgt gcttgaggaa
     1561 ggcaagaccc aggcctttcc ccttcgacgc cccctcgatg atgcccggga tatcggcgat
     1621 gacgaaggtc ttctcattgt catcgaggac ccccagggcc ggggtaaggg tggtgaaggg
     1681 atagtcgccg accttcggtc tcgcctgggt caggcgggag atgagggtcg attttcccgc
     1741 gttgggatgg ccgacgaggc cgacatcggc gaggagcttg agatcgaggt gaagccaccg
     1801 cctctgtccc tcttcgccgg catcgaactc cagaggggcc cggtgggtgg aggtgacgaa
     1861 cctggtgttc cccctgcctc ccctgccacc cagggctgcc gcgtgcgtct cgccatccct
     1921 gaggacctga aacagaagct ctgaggtatc gcggtcgtaa acgacggtgc cgagaggcac
     1981 gtgtattgtg acgtccctgc cttccctgcc ggtcttgttc tttcccgctc cggcttttcc
     2041 gttctcagct ttgtagttgc gcttgtatct gaaatcgtgg aggcttgcga ggtctttctt
     2101 ccctgctatg ataacgtctc ctcccttccc gccatcaccg ccgtcgggcc cgcctcgcgg
     2161 aatgaacttt tctcgtcgga aacttacaca gccactccca ccttttccag actcaacata
     2221 aatgatcgct tcatctatga acttcatcaa acaggtacaa catgcgccct tcttctgtca
     2281 cctgccatgt cgaacttcac gattccgtct atcagggcaa agagggtgtc gtccttgccg
     2341 atgccgacat tctgtccggg atggatcttc gttccgtgct gcctgacgat gatgctgccg
     2401 gcctttatgg cctgaccgcc gtatatcttg acgccgagcc gctggccctg gctgtccctg
     2461 ccgttgcgtg agcttccgcc tgccttcttg tgcgccatgt tatgcctcca ctatgatctt
     2521 ctctacaagc agttccgtga aaggctgccg atggcctctt ttcaccttgt agtcttttct
     2581 tctcttgtac ttgaagaccg taaccttctt gtcccttccc tgcgtcacaa cctttccgtt
     2641 gatatatgcg cctgaaacat aggggcttcc aatgagcgtg gtctctcccg tgttgacggc
     2701 aagaacgttt tcgatcttca cttcgtcgcc ttccgcgccg gcgaacttct cgagcctaac
     2761 tttcttacct tcttcgatct tgtactgctt gccgccgttc tcgatgatcg cgtacatgtg
     2821 atactcctta ccttaggttc tgaaaaaaac tacatatatt atgataagga cgcggaaaaa
     2881 gtcaatagaa aggagtcacc ggcggatggc cccacccaaa actatggact tttgacccgc
     2941 tatccgttat attgatgtgc atcggggcgt agcgcagcat ggtttagcgc acctgcttgg
     3001 ggtgcaggag gccgcaggtt caaatcccgt cgccccgata ttttatttct cgagaccggg
     3061 tacttcttgc ttatccttct caccaacgac gacggcatcc attcaaaggg gatcgtcacc
     3121 ctcggcagat acctgtcgga aaaacacgac gtgtacatga tcgcgccgga gcgcgaacgg
     3181 acatgcgtcg cccacgccgt gacgctgcac aaacccttga gggtccgtga ggtgacgcgg
     3241 agcgcgtacg cgacgaacgg aacgcccgcg gactgcgtcc tcctcggcgt ccacgccgtc
     3301 ctcccgcggc gccctgattt cgtggtctcg ggcataaaca cgggaccgaa catgggccgg
     3361 gacgtgaact actcgggaac ggtggcggcg gcgagagaag gggccttcct gggaatcccg
     3421 tcgatggccg tctccatctg cgctcgtgag gggttcctct tcgacgacgc ggcgggagtc
     3481 gcctgcgccg tcatggagag gcttacggaa ggagtctttc cccagggcac cgtcgtcaac
     3541 atcaatattc ccaaccttcc gcgagagaag ctcaaaggtt tttccgtgac aaggctcggc
     3601 aaaagaatat ataatgatat tgttcacgaa agagtcgatc cacggggccg gaaatactac
     3661 tggataggcg gcaacggtga aagatacgaa cgcaggcgcg gtaccgactt ctacgccata
     3721 gagaagggct acgtttccat cacccccctg ggtctcgata taacagacgc cggttcgatg
     3781 aaaatttatc aaaaacactt caggaggttg ttatgaagac aacgttatcc attatcaaag
     3841 ctgacatcgg tagcatcggc gggcatatca tgcccagcaa gaaactgatc aagcgggtaa
     3901 tagaattcgt agagtccgaa gccaaaggtc tcgtaaacga ctttttcgtg agccacaccg
     3961 gcgatgacgt cgccatcctc ttcgcccaca cgaacggggt cggcttcgag aaactccaca
     4021 agctcgcctg ggacgccttc ctcgcgggga cccaggtggc gaagaaacag gggctctacg
     4081 gagcgggcca ggaccttctg aaggactcct tttcggggaa cgtcaagggc atggggccgg
     4141 ccgtcgcgga gatggagttc gaggagagac cgaacgaacc cttcgtcatg ttcgcggcgg
     4201 acaagacgg
//
LOCUS       DAOQTR010000137         4209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23596_length_4209_cov_3.202215, whole genome shotgun sequence.
ACCESSION   DAOQTR010000137 DAOQTR010000000
VERSION     DAOQTR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23596_length_4209_cov_3.202215"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKN85_04480"
     CDS             <1..291
                     /locus_tag="PKN85_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNT43693.1"
                     /translation="FASCDLIVTPVSPTTAFKVGERLEDPLTMYLSDIFTIPVNLAGL
                     PGISIPGGFDSRGLPIGLQIIGKPLDEGRMLQAAHVFESVKKVPTIPERLMG"
     gene            638..1234
                     /locus_tag="PKN85_04485"
     CDS             638..1234
                     /locus_tag="PKN85_04485"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNT43694.1"
                     /translation="MTALKNMGVDEYVFAERKGPTLSELKKTVLACERCALAKTRKSV
                     VFGEGNPKADIVFVGEAPGEEEDNQGRPFVGRAGKLLDQLIERIGIKRSDVFICNVLK
                     CRPPGNRDPEPDEAAACKEYLLAQLDIIRPRIICTLGRHAYNTLLETDAPITRIRGKL
                     TTFRGVPLLPTYHPSYLLRSQGRIKEAWEDMDKLKTML"
     gene            1530..2783
                     /locus_tag="PKN85_04490"
     CDS             1530..2783
                     /locus_tag="PKN85_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43695.1"
                     /translation="MKRASAIIVSMAFVLLLCSPAGAAGYTYSVVDYPGATHTEAWDI
                     NNSGVIIGWYDANSDYPWEPRGFVLYEATWYSLDYPGAMWTFAMGINDGGTLVGTSVD
                     AGGVAFGYSLTGGGYTYIDPYPRAGESYVYSVNSSNTMVGWFSGERPHVPEGLRGYYG
                     YSLSGGTYTLLDYPVGPGVVFHAYGINDTDTIVGQCVDFQGTHCCSLSGDTLFFLDRP
                     GADSTVAWGINNGGAIVGEYTDATGTHGFLLDGATWTTLDYPGAYETVAHGINDVGVI
                     VGYFTDPSGTHAFVATPATDLMPVAIDIRPWSSRNLINASSSGFVPVAILGSPNFDAP
                     EMTDPGSLTFGRTGEEASMAFCSPWALDVNSDGYADLLCLFRTGTAGFRCGDTRGILR
                     GVTGDGILFEGKGAVRVYPCQTVSR"
     gene            2895..3782
                     /locus_tag="PKN85_04495"
     CDS             2895..3782
                     /locus_tag="PKN85_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNT43696.1"
                     /translation="MRYILGPMKVPFLILTPMCILVGISTAMWSGARVDIVSVVLIVI
                     GAVCSHISVNALNEYCDFRSGLDFRTERTPFSGGSGTLQERPDKAGWALATGILSLVI
                     CFAIGMYFVFTVGVLLLPLGLTGMALIVFYTQWITKRPIVCLIAPGLAFGPLMVLGTH
                     FVLTGSYSLTALLASLVPGFLVSDLLLLNQFPDVEADRTVGRKHLPILLGRKNVARIY
                     IFFLAASYVPLFLGYALGVFPVHVFFALATTVIAVQTSRGVLRYSEEMKSLVPYLGRN
                     VIINLATPALFAIGLMIAS"
     gene            complement(3834..>4209)
                     /locus_tag="PKN85_04500"
     CDS             complement(3834..>4209)
                     /locus_tag="PKN85_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43697.1"
                     /translation="DDEPAILGTLQQIFERSGYEALTAESAEQALDMLKNESVMIMFL
                     DLNLPGMSGVDLCRLIRNKNRIATIHALTGYVNIFGQIECREAGFDDFFVKPVDINVL
                     LKAAESAAEKLRRWKVDEYEVI"
BASE COUNT          856 a         1307 c         1192 g          854 t
ORIGIN      
        1 ttcgcctcct gcgacctcat cgtcacgccc gtctccccga cgaccgcctt caaggtcggg
       61 gaacgcctgg aagaccctct gaccatgtac ctctcggaca tcttcaccat acccgtcaac
      121 ctggcgggcc tgcccggcat ctccatcccg ggaggcttcg attccagggg actccccata
      181 ggtctccaga tcatcggcaa accgctggat gagggaagga tgctgcaggc ggcgcacgtg
      241 ttcgagtcgg tgaagaaggt gccgacgata ccggaacggc tgatggggtg aaggaaaaga
      301 taaaagatcg tctcacgccc gcttcgctcg agggcgcaga gaaaagcgat gagagggcgc
      361 agagaaaaga tcgtcgcatc ccacctgcgg cgggaggcaa agcgggttca caacatatgg
      421 gatctgtcca cggttggttt tggttgggtt ttcctcccgc tcgttagagc gggatcgaag
      481 gttcccggga attcctcaaa ttcccgggaa tgtcttcctg cgtgatctcc ttctttcctc
      541 cgcgtgctcg agtgaggacc gaagggccga acgggcgtga gatgatcttt tgagaggtca
      601 ggtttagacc atggatgcaa gggatgcaaa aggtttgttg acggcgctta agaacatggg
      661 cgtcgatgaa tatgtctttg ccgaaaggaa agggccgacg ctctccgagc tcaagaagac
      721 ggtcctcgcc tgcgagaggt gcgctctggc gaagacacgc aagagcgtcg tcttcggaga
      781 agggaacccg aaggcggata tcgtcttcgt gggcgaggcg ccgggagagg aagaggacaa
      841 ccaggggcgg cccttcgtcg gcagggcggg caagctcctc gaccagctca tcgaacgcat
      901 cgggataaag agaagcgacg tcttcatctg caacgtcctc aaatgcaggc cgccgggaaa
      961 ccgcgatccc gaacccgatg aagcggcggc gtgcaaggaa tacctcctcg cgcagctcga
     1021 tatcatccgg ccccgcatca tatgcaccct gggccgccac gcctacaaca ccctgctcga
     1081 aacggacgcc cccataaccc gcatccgcgg caaactcacg accttccggg gcgtacctct
     1141 cctgcccacc taccacccct cctacctcct gcgcagccag ggcaggatca aagaagcctg
     1201 ggaagacatg gacaaactaa agaccatgct ctaacccccc acccctgggg ggcccatgtg
     1261 tcccatatgt cctaagcgtc ctatctgtcc tattcgtcct ataggtccta ttcatcccac
     1321 aggtcccata ccactccgcc ctgtcaccaa aaaaacgaca ccaaccccac aggaaccatt
     1381 acaaaacaat tgcattttct gcgacagaca gaaatcccgc gttctcgtta tgatatccct
     1441 atccgtcggg accttctggc agtaaggccg gaaagcgaga gacaggagca cggcgcgtca
     1501 ggttcggata cccctcaagg aggagtaaga tgaaacgagc ttccgcaatc atcgtctcca
     1561 tggcctttgt ccttctcctt tgcagcccgg ccggggccgc cggctacacg tacagtgtcg
     1621 tcgactaccc gggagccacg cacacagagg cctgggacat caataattca ggggtgatca
     1681 tcgggtggta cgacgcgaat tccgactacc cctgggaacc tcgcggcttt gtgctgtacg
     1741 aagcgacctg gtacagcctc gactacccgg gagccatgtg gacatttgcc atggggatca
     1801 atgacggggg aacgctcgtc gggacctcag tggatgccgg cggcgtggcc ttcggatatt
     1861 ccctgacagg cggtggctac acgtacatcg acccgtaccc ccgggcggga gagagctacg
     1921 tctattcagt caacagttcg aacaccatgg tcgggtggtt ctcgggggaa agaccccacg
     1981 tgcccgaggg gctgcgcggc tactacggct attcgctgag tggcggcacc tacacccttc
     2041 tcgactatcc agtgggtccg ggggtggtct tccacgctta cggcataaac gatacagaca
     2101 ccatcgtagg acagtgcgtg gacttccaag gcacgcactg ctgctcgttg agcggcgaca
     2161 cgctcttctt cctcgaccgt cccggagcgg attccaccgt cgcctggggt atcaataacg
     2221 gcggggccat cgtcggagag tacaccgacg cgacgggaac gcacggcttc ctgctcgacg
     2281 gcgccacatg gacgaccctc gattaccccg gagcatacga gaccgttgct catgggatca
     2341 atgacgtcgg ggtgatcgtg gggtacttca ccgatccctc gggcacccat gccttcgtgg
     2401 ccacccccgc gacggatctc atgcccgttg cgatcgacat caggccctgg agcagcagga
     2461 acctcatcaa cgcaagcagc tcgggcttcg tccccgtggc gatcctcggg agccccaatt
     2521 tcgacgcccc tgagatgacg gacccgggca gtctcacctt cggacgtacg ggagaggagg
     2581 cgagcatggc cttctgcagc ccctgggccc tggatgtaaa ttccgacggg tacgccgacc
     2641 ttctgtgtct cttcagaaca ggcacggcag ggttccggtg cggagatacc cggggtatct
     2701 tgaggggcgt aaccggggac gggatcctct ttgaggggaa aggcgccgtc agggtctacc
     2761 cctgccagac agtctcccgc tgagtctaaa agggtcgtct cgtgccttcc cgatcatgtt
     2821 ccatctttca ctccttgcaa aaacaggcct gattctgtat gattaggaac ttattcgtat
     2881 cgggagggtg aaaaatgagg tacatactcg ggcccatgaa ggtccccttt ctgatcctca
     2941 caccgatgtg catcctcgtg gggatatcga ccgccatgtg gtcgggggca cgggtcgata
     3001 tagtctccgt tgtcctcatc gtcataggcg cggtatgctc ccacatcagc gtcaacgccc
     3061 tcaacgaata ctgcgatttc aggagcggtc tcgatttcag gactgagagg acccccttca
     3121 gcggcggcag cgggacgctt caggagaggc ccgacaaggc gggatgggcc ctcgcgacag
     3181 gcatactatc cctggtcatc tgtttcgcca tcgggatgta cttcgttttc accgtcggcg
     3241 tgctcctgct tcccctgggt cttacgggca tggcgctcat cgtcttctat acccagtgga
     3301 tcacaaagag gcccatcgtc tgcctcatcg cacccggcct tgccttcggc cctctcatgg
     3361 tgctgggcac gcatttcgtc ctcacaggca gctactcctt gaccgctctc cttgcgtccc
     3421 ttgtcccggg gtttctcgtg agcgacctgc ttcttctcaa ccagttcccc gacgtggaag
     3481 ctgacaggac ggtgggaagg aaacacctgc ccatcctcct gggaagaaag aacgtggcaa
     3541 ggatatacat attcttcctt gccgcctcat atgtaccgtt attcctcggc tacgccctcg
     3601 gcgtcttccc cgttcatgtc ttcttcgccc tcgccacgac ggtcatagcc gttcagacat
     3661 cccggggcgt cctgagatac tctgaggaaa tgaaaagtct tgttccctat ctgggcagga
     3721 acgtgatcat caacctcgcc accccggcgc ttttcgccat aggcctcatg atcgcctcgt
     3781 aatgccgggc ccccggcggc ggcactcgga aagggcagga tcgcaatggc gtcctagatc
     3841 acctcgtatt cgtcgacctt ccaccggcgc agtttttccg cggcgctttc ggccgccttc
     3901 aggagaacgt tgatgtccac gggcttgacg aagaaatcat caaagcccgc ctcacggcac
     3961 tctatctgac cgaagatgtt cacgtagccc gtaagggcat ggatggtcgc aatacggttc
     4021 ttgttgcgta tcagcctgca aagatcgaca ccgctcatcc cgggaaggtt gaggtccagg
     4081 aacattatca tgacggactc gttcttcagc atgtcgaggg cctgctcggc gctttcagcc
     4141 gtcagggcct catagccgct cctttcgaat atctgctgca gcgtaccgag aatggccggt
     4201 tcgtcatcc
//
LOCUS       DAOQTR010000138         4192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_23731_length_4192_cov_3.965434, whole genome shotgun sequence.
ACCESSION   DAOQTR010000138 DAOQTR010000000
VERSION     DAOQTR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23731_length_4192_cov_3.965434"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKN85_04505"
     CDS             complement(<1..540)
                     /locus_tag="PKN85_04505"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT43698.1"
                     /translation="MKSIILLLWIPPLVYLTFSVFYYAFLAIAYFVTKDKEKAAAPGT
                     RRYLLLIPAHNEEALIGRALASLTRVDYDRDRFEACVIADNCTDGTAGIAAQHNARVL
                     ERHDASRKGKGFAIEWAMKQVDLGAFDAVVIADADNLIDPGFFRGLDEVLDGGRRAVQ
                     CNNCLANSRDTVFTGVIHLS"
     gene            complement(617..2740)
                     /locus_tag="PKN85_04510"
     CDS             complement(617..2740)
                     /locus_tag="PKN85_04510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT43699.1"
                     /translation="MDKLRVGIVGCGTIFHHHQAFIRSYPGATLCAVADRDEKALKKA
                     SDVYGIANCYSDLEEMARAEATDVIHITTPPQTHAALAEKAMNLKNHVFVEKPLTLDH
                     RSAVRLYDIAAHNGVKLCVDHNHLFDPWMLKAKEVLKDLRPGDVTYVESYYGINPRIP
                     EIMGYREAGEISWIFSLPGGLFHDFLSHPLYLMLEYTGRPVKMQTMAGSCGALIQDLS
                     DELHIMVEGEKAMGKLTISFNARPFQHFLKIYHKKAIVTVDFNNMTMVANRLTGLPGA
                     VTKIAGNLGAARALTTQTFSNVYRFVTGKLKPYSGMKNLMHGFYDSILQATDTPVPAE
                     SALAVLKVMDEVWKDAGKLHPVFRNVSARAEVEWAPARGRVLVTGAGGFLGRRLTEVL
                     VEKGYFVRVLVRRLTDVDPFRALGVDVHYGDVRDELALAQAIDGMDFVVHAAAAQEGD
                     WDTFDATTVRGTEKVMRLARQLKVRRVIYISSMSVYHVNGLRKGSVITEDGRLEENPG
                     ARGFYTFSKLEAEKVAKGLMATNGNGNGKVPGVILRPATIYGPRGPVFTPLIGVSLFD
                     RVFMILGKKKMRLPLVYIDNLVDAIALCMEDDRAAGQTFNVIDDEGTTKEGYLKKLAS
                     EVFPRSRSVSLPYWFVHSAVHFQEAVFRMMHRDPVLTRYRLGSASADAAFSNAKIKES
                     IGWQPRVSLEEGLSRTFEWFKENGE"
     gene            complement(2733..3878)
                     /locus_tag="PKN85_04515"
     CDS             complement(2733..3878)
                     /locus_tag="PKN85_04515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT43700.1"
                     /translation="MTGPITVLHLIAPAHFGGAERVVLSLADAIDRSRFNVLIGSFVN
                     ARFPDNEFLGQVREKGISGTVFWLRRTLDIENIPRLVRFIRSEGVAIVHTHGYRSDIV
                     GLIAARLSSRPVIATIHGFVPIDSRLRVYEQCDRFALRFFDLVLPVSDQIERGLCASG
                     VRRERMTTIRNAIAPGGGDGPKRSASPLPLLKRDGDFLIGVVGRLSPEKNIPGFLEAA
                     RDLSARYGHLRFVVVGEGPEREALKGLTTRLGLDGKVRFTGFVEEMEEVYSLLDMLVI
                     SSSTEGVPLTVLEAMRHGVPVVSTGVGGIPEVIEDGVDGLIVESGDTGALCRAVETLV
                     VDGNRYMAISRNAQKKVLRDFDRSSWARRIERCYLSMLNKREESSNG"
     gene            complement(3890..>4192)
                     /locus_tag="PKN85_04520"
     CDS             complement(3890..>4192)
                     /locus_tag="PKN85_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNT43701.1"
                     /translation="IGHGSIIAWDCVIMDRDYHSTDNGPELVRPVTIGNGVWIGCRAI
                     ILKGVTIGEGAVVAAGAVVTKDVSPFTLVAGNPARAVKRVAGWRKGRPSLTISRKT"
BASE COUNT          813 a         1349 c         1188 g          842 t
ORIGIN      
        1 cgaaaggtgg atgaccccgg tgaaaaccgt gtcgcgggaa ttggcaaggc agttgttgca
       61 ctggacggcg cgtcttcccc cgtccaggac ctcatcgagg ccgcggaaga acccggggtc
      121 gatgaggttg tccgcgtcgg cgatgacaac cgcgtcgaaa gcaccgaggt cgacctgctt
      181 catggcccac tcaatggcaa aacccttgcc cttccttgaa gcatcgtgtc tctcgagcac
      241 cctggcattg tgctgtgcgg caatcccggc tgtcccgtcg gtgcagttat ccgcgatgac
      301 gcaggcctca aaacgatccc ggtcgtagtc cacccgggtg aggctcgcga gggccctccc
      361 tatgagggcc tcctcgttgt gagccggtat cagaaggagg taccggcggg tccccggcgc
      421 ggcggccttc tctttgtcct tggtcacgaa atacgcgatt gcaaggaaag cgtagtagaa
      481 gacactgaag gtgagataaa cgagcggcgg gatccacaac agcaggataa ttgacttcac
      541 ccggcaaacc tccgcgagac cgttctacgt tctgcggttg ccattaccca aacccatagc
      601 ccatcaccca tcgccctcat tctccgttct ccttgaacca ctcgaaggtc cttgacagac
      661 cttcctcgag ggataccctg ggttgccatc cgatggactc cttgatcttc gcgttgctga
      721 aggccgcgtc cgcggaggcg gagccgagcc ggtaccgggt cagcacgggg tccctgtgca
      781 tcatcctgaa gacagcctcc tggaaatgga ccgccgagtg cacgaaccag tagggcaggg
      841 acaccgaacg ggaccggggg aagacctctg acgcaagctt cttcaggtag ccctcttttg
      901 tcgtcccctc gtcgtcgatg acgttgaacg tctggcccgc cgccctgtca tcctccatgc
      961 acagggcgat tgcatccacg aggttgtcga tgtaaacgag gggcagcctc atcttcttct
     1021 tccccagtat catgaacacc ctgtcgaaga gcgaaactcc gatgagcggc gtgaaaacgg
     1081 ggcccctcgg cccatatatg gttgctggcc tcagtatgac ccccgggacc ttgccgttac
     1141 cgttaccgtt cgtcgccatc aaccccttcg ccaccttttc cgcctccagt ttcgagaagg
     1201 tgtagaatcc ccgggccccg gggttctctt caagccgtcc gtcctccgtt atcaccgaac
     1261 ctttcctcag gccgttcaca tggtaaacgc tcatggaact gatatagatc acccgcctga
     1321 ccttgagctg ccgggcaagc ctcatgacct tttccgtccc gcggaccgtc gtcgcgtcaa
     1381 aggtgtccca gtcgccctcc tgggcggcgg cggcatggac cacaaaatcc atcccgtcga
     1441 tggcctgcgc cagggcaagt tcgtcgcgca cgtcgccgta atggacgtcg acgccgaggg
     1501 ccctgaaggg atccacatcc gtgagcctcc ggacgagaac gcggacaaaa taccctttct
     1561 ccacgagaac ctccgtcagc cttcgaccca gaaagcctcc cgcccccgtg acgagcaccc
     1621 ttcccctggc tggggcccat tccacttccg ccctcgcgga gacgttcctg aatacgggat
     1681 ggagcttgcc ggcatccttc catacctcgt ccatgacctt gagcacggca agcgcgcttt
     1741 ccgcgggaac gggggtgtcc gtcgcctgca ggatggaatc atagaaaccg tgcatgaggt
     1801 ttttcatccc cgaatagggc ttcagttttc ccgtaacgaa cctgtacaca ttggagaacg
     1861 tctgcgtggt cagcgcgcgg gccgcgccga gattgcctgc tatcttcgta accgcccccg
     1921 gcaaccccgt cagcctgttg gctaccatgg tcatattgtt gaagtcgacg gtgacaatgg
     1981 ccttcttgtg gtagatcttc aggaaatgct ggaaaggcct cgcgttgaag gagatggtca
     2041 gcttgcccat ggccttttca ccctccacca tgatgtggag ctcgtcggac aggtcctgga
     2101 taagggcccc gcacgagccg gccatcgtct gcatcttcac gggcctgccc gtgtactcca
     2161 gcatgaggta gaggggatgg gaaaggaaat catggaaaag gcctcccggc agggaaaaga
     2221 tccaggatat ctcgcccgcc tcccggtaac ccatgatctc ggggatgcgg gggtttatgc
     2281 cgtagtagct ttccacgtag gtgacgtccc cgggacggag gtctttcaat acctccttcg
     2341 ccttcagcat ccagggatcg aagagatggt tgtggtcgac gcacagcttc acaccgttgt
     2401 gcgccgcgat gtcgtaaagc ctcacggcgc tcctgtggtc gagtgtcagc ggcttctcga
     2461 cgaagacgtg gttcttcagg ttcatggcct tctcggccag cgcggcgtgt gtctgagggg
     2521 gcgtggtgat gtggatgaca tccgtcgctt ccgcgcgggc catctcctcg aggtcactgt
     2581 agcagttcgc gatgccatac acgtcgctcg ccttcttcag cgccttctcg tccctgtccg
     2641 ccaccgcgca cagggtggct ccgggatagg agcgaatgaa ggcctggtgg tggtgaaaga
     2701 tcgttccaca accgacaata ccgactctga gcttatccat tcgacgattc ctccctttta
     2761 ttgagcatag acagataaca tctttcgatc ctccgcgccc atgaagaacg gtcaaaatcg
     2821 cgaaggacct tcttttgagc gttgcgggat atcgccatgt acctgttgcc gtcgacgacg
     2881 agcgtttcta cggcccggca gagcgccccc gtgtctcccg actccacgat caggccgtcc
     2941 acgccgtcct cgatgacctc cggtattccg cccaccccgg tggaaaccac cggtacaccg
     3001 tgcctcatcg cctcgaggac tgtgagcgga accccttccg ttgacgagga gatgacgagc
     3061 atgtccagca acgaatagac ctcttccatc tcctcgacga acccggtgaa tctcaccttt
     3121 ccgtcgagac ccaacctcgt cgtcaggccc ttgagggcct cccgttccgg tccttcgccg
     3181 acaacgacga acctgagatg accgtacctt gccgacaggt ccctggccgc ttcgagaaac
     3241 cccggtatgt tcttctcggg gctcagcctt ccgacaacgc ctatcagaaa gtctccgtca
     3301 cgcttgagaa ggggaagcgg ggaggccgaa cgcttcggcc cgtcaccgcc cccgggtgcg
     3361 atggcgttac ggatcgtggt catcctctcc ctgcgcacac cgcttgcgca caacccccgc
     3421 tctatctggt ccgagacggg tagcacaaga tcgaagaagc gcagcgcgaa gcgatcacat
     3481 tgctcgtaga cgcgcaaccg cgagtcgatg ggcacgaagc cgtggatcgt cgcgatcacg
     3541 gggcgggacg acagcctggc ggctataagt ccgacaatat cggagcggta cccgtgggta
     3601 tgaacgatgg caaccccttc ggaacggatg aacctcacga gacggggaat gttctcgatg
     3661 tccagggttc gcctcaacca gaagacggtt ccggagatcc ccttctcacg cacctgcccc
     3721 aggaactcat tgtcgggaaa acgggcattc acgaaggaac cgatgagaac attgaaccgc
     3781 gaacggtcta tggcgtccgc aaggctgagg acgacccttt ctgcccctcc gaaatgcgcc
     3841 ggggctataa ggtgcaagac ggtgatcggt cctgtcaacg gttctcctgt catgtcttcc
     3901 gtgagatagt gagggagggc cggcccttgc gccaacccgc cacccttttt acggcccgcg
     3961 ccgggttgcc cgcgaccagg gtgaagggag agacatcctt tgtcaccacg gcgcccgctg
     4021 ccacgaccgc cccctcgcct atggtcaccc ctttgaggat gatggcccgg caaccgatcc
     4081 agaccccgtt cccgatcgtc acgggcctga ccagctcggg accattgtcg gtcgaatggt
     4141 agtcccggtc catgatgaca cagtcccagg ctatgatcga gccatggccg at
//
LOCUS       DAOQTR010000139         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24016_length_4155_cov_3.306098, whole genome shotgun sequence.
ACCESSION   DAOQTR010000139 DAOQTR010000000
VERSION     DAOQTR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24016_length_4155_cov_3.306098"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKN85_04525"
     CDS             complement(<1..358)
                     /locus_tag="PKN85_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43702.1"
                     /translation="MTTAIEDFLREDVGTDDVTTNAIVPAQHRSRAKIVAKAPGVIAG
                     QGFAKEVFMTLDRDIAYREHREDGEAVSPGDVIAAVEGATRAILTAERVALNILQRLS
                     GIATLTKVFVDAASGTK"
     gene            489..562
                     /locus_tag="PKN85_04530"
     tRNA            489..562
                     /locus_tag="PKN85_04530"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:523..525,aa:Lys,seq:ttt)
     gene            575..649
                     /locus_tag="PKN85_04535"
     tRNA            575..649
                     /locus_tag="PKN85_04535"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:611..613,aa:Glu,seq:ttc)
     gene            923..>2068
                     /locus_tag="PKN85_04540"
     CDS             923..>2068
                     /locus_tag="PKN85_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43703.1"
                     /translation="MDYTSRPRREITDELSALRKRVEELERSARDRKNIEDALRQSEA
                     RYKRLVVNLPGVVYQFMMGPDGSFSFPFVNENLSEITGLSAGDVMRDASRILDMIPPE
                     DMERFREEVFESARSLRPYHTILRLLKGTGYIWVEARSTPERMPDGSVLWDGFFLDIT
                     ERKEKQESLRKTQFTMDRAPDSILWVNDEGRIVYANDRASEWTGFTREELLGMPIFEL
                     DPDFLLENWEGHKEDMKRLGLLSFEGRHVRKDGSTFPVEVSTNYLEVDGHFMSCAFDR
                     DISVRKEAEKALLESEAKFRDLAEKSVAGVYLIQDGIVRYVNARISEMLGYPMDEMID
                     RMRLKDVIYPDDWAVVEANMQRRLTGELVPENYEFRLVTRQGEIRTVE"
     assembly_gap    2069..2078
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2079..2941
                     /locus_tag="PKN85_04545"
     CDS             <2079..2941
                     /locus_tag="PKN85_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43704.1"
                     /translation="QGEPAVIGTLLDITERKEAERKIIESERSLSAILAASPIGIGMA
                     RDRKILWVNERVCRFTGYTQEELQGRSASIFYESPEEYERVGEILYREEEARTKMVRV
                     DGEVRDVLIQLCFTDPDSCIFTVVDITEQQRAEESLRFTQFAMDRARDLIIWVRDDGS
                     IFYVNDAACESLGYDREELLKMTVFDLDRTYPREAWAPAWTRLRQNGSLLLEVRPTRK
                     DGSVFSLEVSASFLSYGGMEYACAFMRDITERRQMEDTLRMSEERFHALFEAAGDAIV
                     SDHRLQRKNT"
     gene            2970..>4155
                     /locus_tag="PKN85_04550"
     CDS             2970..>4155
                     /locus_tag="PKN85_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43705.1"
                     /translation="MGKTLVDFSPESQTGGGKSVEEASAKTRACLQGIPQFFEWKYLR
                     ADGTPFDAEVSLTRIDIRGEVYLQAIIRDITGRKRTEEDLRRLSIAIEQAAEDVIITD
                     AEGVIQYVNPAFEAILGYSREDAIGQTPRLVKSGVHDTAFYEDLWNTIKGGDIWTGQI
                     TNRRKDGALIHEDAIISPLMDSSGGLTGYISLKRDITEEVKLHSQLRQAQKMESVGTL
                     AGGIAHDFNNILTALIGYATIVQMKMEKDNPLMPYIDQVLSASEKAADLTRSLLTFSR
                     QQPITLAPLDLNDAIRATEKLLKRLLTEDIDLDTSLAGERTVIMADRSQIDQILFNLV
                     TNARDAMPKGGVLKIGTSVVDMDRKLIGTFGFGKAGRYVLVSVSDTGTGMDAATREKI
                     FDP"
BASE COUNT          934 a         1098 c         1278 g          835 t
ORIGIN      
        1 ccttcgtgcc cgacgcggca tcgacgaaga ccttcgtcag tgtggcgatc cccgagagtc
       61 tctggaggat attgagggcc acccgttcgg cggtgagtat cgccctcgtg gcgccctcca
      121 ctgccgcgat gacatcgccg gggctgacag cctcgccgtc ctccctgtgt tcgcggtacg
      181 cgatgtctct gtcgagggtc atgaagacct ctttggcgaa tccctggccc gcgatcaccc
      241 ccggggcctt cgcgacgatc ttcgcacggg agcgatgttg cgcgggcacg atggcgttcg
      301 ttgtcacatc atctgtccct acatcttccc tgagaaaatc ctcgatcgcc gtggtcatgg
      361 tggaatatta taaggtgacc atcagggcta gtcaatgaaa gactgtgtgg aagaccgcgt
      421 gaagactgtt gatcaggttc attctgcttg actttgggga aaaattttca tatgttaatg
      481 gacgttttgg gccgttaact cagtctggta gagtatctgc cttttaagca gagagtcgaa
      541 ggttcaagtc cttcacggcc catatgacct tagggtcccc atcgtctagc ctggtccagg
      601 acaccggcct ttcaagccgg caacgggggt tcgaatcccc ctggggacgc tttcccacac
      661 ctcccaccag ttgatgtctt tctcagaatg ccgccatcgt atccgaaatt cgggaggtcc
      721 ccgacagtgg cgaccccgaa cgctctggcg taaggggtgt cttttatttc ccggcgtatt
      781 gtgcgataac ttttatgata cgcgcgtttc ccggaccgcg tgccgccttt cgtcgcggac
      841 attccgggga gcaggcggac cggcgcggcg tatcttgtgt tgctggtgta taacaagaga
      901 atgacagcga gggaaaagcg cgatggatta tacatcaaga ccgcgtcggg aaatcactga
      961 tgagctgtcg gccctcagga aaagagtgga agaactggaa agatccgcgc gcgaccgcaa
     1021 gaacatcgag gacgcgctcc ggcaaagcga ggcgaggtac aagagacttg tcgtgaacct
     1081 gcccggggtg gtgtatcagt tcatgatggg ccccgacgga tctttttctt ttccctttgt
     1141 caacgagaac ctgtcggaga tcaccggttt gtccgcgggg gacgtgatgc gcgacgcctc
     1201 acgcatcctt gacatgatac cacccgagga tatggagcgg tttcgggaag aagtgtttga
     1261 atcggcacga tcccttaggc cttaccacac catcctgaga cttttgaagg gtaccgggta
     1321 catctgggtc gaggcgcgtt ccacgccgga gcgcatgccc gacgggagtg ttctctggga
     1381 tggttttttt cttgatatca cggaacgcaa ggaaaagcag gaatcactcc gcaagacgca
     1441 gttcacgatg gaccgcgccc cggacagtat cctgtgggtg aacgacgagg gccgtatcgt
     1501 ctatgccaat gatcgcgcca gcgagtggac cggattcaca cgggaagagc tcctggggat
     1561 gcccattttt gagctcgatc ccgacttcct tctcgagaac tgggagggcc acaaggagga
     1621 catgaagcgc ctcgggctcc tgtcattcga gggtcgtcat gtcaggaagg acggcagcac
     1681 ctttcctgtt gaggtcagca cgaactatct ggaggttgac gggcacttta tgtcctgcgc
     1741 ttttgaccgg gacatctcgg tccggaagga ggcagagaag gcgcttctcg aatcggaggc
     1801 caagttccgg gacctggccg aaaaatccgt ggcgggcgtc tacctgatac aggatggtat
     1861 tgtcaggtat gtcaatgcca ggatatcaga gatgctggga taccccatgg atgagatgat
     1921 cgacaggatg cggctcaaag acgtgatcta ccccgatgac tgggcggttg tcgaggcgaa
     1981 catgcaaagg aggcttaccg gggaactggt gcccgagaac tacgaattca ggttggtgac
     2041 aagacaggga gagatcagga cagtcgaann nnnnnnnnat cagggagaac ccgccgtcat
     2101 cgggacattg ctcgacataa cggaacgtaa ggaggccgag aggaagatca tcgagtccga
     2161 gaggtcgttg agcgcgatcc tcgctgcttc tccgataggg atcggcatgg ccagagacag
     2221 aaagatccta tgggtcaatg aaagggtgtg caggttcacg ggttacacgc aggaggaact
     2281 gcagggccgg agtgcttcca tcttttatga aagtcctgaa gaatatgaga gggtgggcga
     2341 gatcctgtac cgcgaggaag aggctcgaac gaaaatggtg agggtggacg gcgaggtccg
     2401 tgacgttctc atccaattgt gttttacgga ccccgactcc tgcatcttca ccgtcgtcga
     2461 catcacggag cagcaacggg ccgaggagtc gctgcggttc acccagttcg ccatggaccg
     2521 cgcgcgggac ctgatcatct gggtgaggga tgacggttcg atattctatg tcaacgatgc
     2581 cgcctgcgag tccctcgggt atgaccggga ggaacttctc aagatgaccg ttttcgatct
     2641 ggaccgcacc tatccccggg aggcatgggc gcctgcgtgg acgaggctca ggcagaacgg
     2701 ttcgttgctc ctcgaagttc gaccgacgag aaaggatgga agcgtttttt cccttgaggt
     2761 cagcgcgagc tttctaagtt atggaggtat ggagtatgcc tgcgctttca tgcgggacat
     2821 cacggagcgc aggcagatgg aggacaccct gcgcatgagc gaggagcgct tccacgccct
     2881 gttcgaggcg gcgggtgatg ccatagtgag cgatcatcga ttgcaacgga aaaacacttg
     2941 agatgttcgg ttgcagccgc aatgagatcg tgggaaagac gcttgtggac ttttcgcctg
     3001 agagccagac cggcggggga aagtccgtcg aagaggcctc ggcgaagacg agggcgtgcc
     3061 ttcagggaat tccgcaattc tttgaatgga aatacctccg tgccgacgga acaccctttg
     3121 acgccgaggt gagcctcaca aggatcgaca tccgcggaga ggtgtacctg caggccatca
     3181 ttcgggacat cacgggaaga aaacgcacgg aagaggactt gaggcgcctc agcatcgcaa
     3241 tcgagcaggc tgcggaggat gtcatcatca cggacgcgga aggggtgatc cagtacgtga
     3301 accccgcctt cgaggcgatc ctggggtatt cacgggagga cgccatcggc cagacgccga
     3361 gactggtcaa gagcggtgtc cacgacacag ccttctacga ggacctgtgg aacacgatca
     3421 agggcggaga catatggacc ggccagatca ccaacaggcg aaaagacggt gctctcatcc
     3481 acgaggacgc gatcataagc cctttgatgg actcctcggg aggtctcacg gggtatatct
     3541 cactgaagag ggacataacg gaggaggtca agctccattc gcagctccgg caggcccaga
     3601 aaatggagtc agtcggtacc ctggccggag gtatcgccca cgatttcaac aacatactaa
     3661 cggctctcat cgggtatgcc accatcgtgc agatgaagat ggaaaaagac aacccgctca
     3721 tgccctatat cgatcaggtc ctttcggcct ccgagaaggc ggccgacctg acccggagtc
     3781 tcctgacctt cagcaggcag caacccatca ccctggcgcc tcttgacctg aacgatgcta
     3841 tccgggcgac ggagaagctt ttgaagagac tgcttaccga agacatcgac cttgacacga
     3901 gcctcgccgg agaaagaacg gtaatcatgg ccgacaggtc ccagatcgac cagatcctct
     3961 ttaatctggt cacaaacgcg cgggatgcca tgccaaaggg aggggtgcta aagataggga
     4021 cgtctgtggt cgatatggac cgcaaattga tcggcacctt cggcttcggg aaggcgggac
     4081 ggtacgtgct cgtcagcgtt tccgataccg gcacaggcat ggacgcggca acccgcgaga
     4141 agatattcga cccct
//
LOCUS       DAOQTR010000140         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24079_length_4147_cov_3.319404, whole genome shotgun sequence.
ACCESSION   DAOQTR010000140 DAOQTR010000000
VERSION     DAOQTR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24079_length_4147_cov_3.319404"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1069
                     /locus_tag="PKN85_04555"
     CDS             83..1069
                     /locus_tag="PKN85_04555"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT43706.1"
                     /translation="MGAMDFLKDVHGRLLEYWILVFILCISAAIGLGQRSLWAGVLTF
                     LGIIAGIAALIGTGAFFGRLGYRGRLRKLRKKLLSPDPRIRCEAAGGLGELWHRGQEP
                     IDDLEGLLDDPDEKVRFQAALSLANFREDGAAGAGELAKRVGTTDHALRLQAVDALGK
                     MGEGAREALPELLEVLCDRNWPDRWQAAAAIVFLRPEGHDAVSALVSVLEEEDGYTAE
                     WAAKALGVITPAATDAVPALVKAVADKGRDVRGAAMESLGAMGEKAIPAVGVLTGALG
                     DKDSWIRGMAASALGNIGRAAAEAVPRLRSMAADPDEWVRETAGKALARIEG"
     gene            1124..3217
                     /locus_tag="PKN85_04560"
     CDS             1124..3217
                     /locus_tag="PKN85_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT43707.1"
                     /translation="MKDEYSSRVYDALFRPESIVVIGGSESLSKPGGKVLANIVEHHY
                     RGTLWVVNPGSGEVMGVPAFPSVTDLPGVPELGIIAIPAKLVAGALDDLGRKGTKAVV
                     ILSAGFGEKGEEGRREERRLLAIAEGFGMTIIGPNCSGFMTFHYSGKFAGIIPELKPR
                     TIDFISGSGATVDLVMEQAVLRGLAFCNVVNVGNSIQTGVEDLIALYDENYDVEGSPI
                     LMVYLESLKKPAVLLRHARSLTQKGCAIVGIKSGVSTSGARAAASHTGAMATDDRAVE
                     ALFDKAGIIRVRSKMEMIDVACSLRGTGGRLKGNRACVITDAGGPGVMLTDELERQGL
                     VLPLLGDRTRGRLHGILPAESSVVNPIDCLPSRTAAQIREIFQVLAEEEGGNIDVIAI
                     QVGNPGIYPNREIYREVARAMKTSPIPVIPTLSSVTTCTEPIAEFTSDGNLCFTDEVN
                     LGSALGKVLHRREIFEVSGDLRNYDPAGIGNALRGQRGVLPARTVTEVLEKAGFVFPP
                     QVEAVSLEEMKEACARIGYPLAMKVLGPLHKSDLGGVKLGIADAAAAERAWHDLTGIE
                     DARGVLVQPMVEGTEVILGASRAGELGHLVMFGLGGIYTEVLRDVTFALAPLAGEECR
                     GMVKGIRSYPILEGVRGQRAVSVERLADHLKRLGRLVFDFPVIGEIDINPLKGSGDEL
                     YVVDARIVLYAAPHS"
     gene            complement(3278..>4147)
                     /locus_tag="PKN85_04565"
     CDS             complement(3278..>4147)
                     /locus_tag="PKN85_04565"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43708.1"
                     /translation="EKTTSRGEQFVGVGVDTLPIIPRDNTDRNRTSPFAFTGNKFEFR
                     MVGSAQSIAGSNVALNTIAAEALDEIATRLEKARDKNAEAAAIIKDTWKKHSRIIFNG
                     NNYSDEWVAEAEKRGLPNVANTVDALKAFVTDKAFKLFEKYDVLSRKELHSRYDIYVE
                     TYAKQINIEARVAIDMVRKQFIPAALEYATFLADSIDDLKSVKGPAAVPEDILKKLSA
                     ILNSAYKNLGKLEAAVEKAQAVSDTVKQAESYRDKVFATVQALRADIDSLEMLVPADT
                     WPVPTYTDLLFKL"
BASE COUNT          768 a         1155 c         1399 g          825 t
ORIGIN      
        1 ggaggcccgt tcatgaaaaa gaaatcaatt gacattcaac acagttgctg gaaccctcga
       61 accgtttcct gtcgggggcg taatgggcgc gatggatttt ctgaaagacg tacacggaag
      121 actcctggag tactggatac ttgtcttcat cctgtgtata tcggcggcaa tcggcctcgg
      181 tcagcggtcc ctgtgggccg gtgtgctcac tttcctgggc atcatagcgg gcattgccgc
      241 cctcatcggc accggcgctt tttttggaag gctcgggtac cggggccgct tgaggaaatt
      301 gagaaagaag cttctgagcc ccgaccctcg catccggtgt gaggccgccg ggggtctggg
      361 tgagctttgg caccgcgggc aggaacccat agatgacctc gaggggctgc tcgatgaccc
      421 cgatgagaag gttcgattcc aggcggcact ttcgctggcg aatttcaggg aggacggagc
      481 agcgggtgcc ggagagctgg caaagcgcgt cgggaccacc gatcacgccc ttcgcctcca
      541 ggcggtggat gccctgggga agatggggga aggggcgagg gaggctctgc cggagctcct
      601 ggaggtgctc tgcgacagga actggcccga ccggtggcag gcagcggccg cgatcgtctt
      661 ccttcggccc gagggtcatg atgccgtatc cgcgctcgtc agcgttctcg aggaggagga
      721 tggctatacg gcggaatggg cggcaaaggc cctgggggtt attaccccgg cggcaacgga
      781 tgccgtgccg gcccttgtga aggccgtcgc ggataagggc cgcgatgttc gcggggcagc
      841 gatggaatcc ctgggggcca tgggtgaaaa ggcgatacct gcggtcggtg ttctcaccgg
      901 cgcgctcggg gataaggaca gctggatacg gggcatggcc gcatcggcgc tgggcaacat
      961 cggccgcgcg gccgctgagg ccgtcccgag gctgagatcc atggcggccg atcccgatga
     1021 atgggtccgt gaaacggcag gaaaggcact ggcaaggatc gaaggatagg cgccgcgatc
     1081 gcccgggagc aaggcgggga ccgtggtcaa gagggagtca ggtatgaagg atgaatattc
     1141 gtcgcgcgtg tacgacgcgc tcttccggcc cgaaagcatt gttgtcatcg gagggagtga
     1201 gagcctgagc aaacccggag gaaaggtgct cgcaaatatc gtcgagcatc actaccgcgg
     1261 gaccctctgg gtggtcaatc ccggcagcgg tgaggtgatg ggagtacccg cgtttccgtc
     1321 ggtgacggac cttcccgggg tcccggagct cgggattatc gcaatcccgg cgaaactggt
     1381 ggccggggcc ctcgatgatc tcggccgcaa ggggacaaag gccgtcgtta tcctttcggc
     1441 cggttttggc gagaagggag aggagggtag gcgggaggag aggcgcctgc tggccattgc
     1501 cgaggggttt ggaatgacga tcatcggtcc caactgctcc gggttcatga cattccatta
     1561 cagcgggaaa tttgcaggca tcatcccgga gctgaagccg aggaccatcg atttcatcag
     1621 cggatccggc gccaccgtgg acctcgtcat ggagcaggcc gtcctcaggg gcctcgcctt
     1681 ctgcaacgtc gtgaacgtgg ggaattccat acagacgggc gtggaggacc tcatcgccct
     1741 ctatgacgag aactacgatg tcgaggggtc ccccatcctg atggtctacc tggagtctct
     1801 gaagaagccg gcggtcctcc tcaggcacgc gcgcagcctg acacagaaag ggtgcgccat
     1861 cgtgggcatc aagtctggcg tctccacctc gggggcaagg gcggcggcaa gccataccgg
     1921 cgccatggcc accgacgaca gggcagtaga ggccctcttc gacaaggcgg gtatcatcag
     1981 ggtgcgcagc aagatggaga tgatcgatgt cgcatgctcc ctgcggggaa cgggcgggcg
     2041 gcttaagggc aaccgtgcct gtgtcatcac cgacgccggg ggtccgggtg tcatgctcac
     2101 cgacgaactg gaaaggcaag gtcttgtcct gccgctcctt ggcgacagga cgcgcggcag
     2161 gctgcacggt atactccccg ctgagagctc cgttgtgaac cccatcgact gtctcccatc
     2221 gaggacggcg gcccagatac gggagatatt ccaggtgctc gcagaggaag aagggggcaa
     2281 tatcgatgtt attgccatcc aggtggggaa tccggggatt tatcccaaca gagagatata
     2341 ccgcgaggtg gcccgggcga tgaagacatc tcccattccc gtcatcccga cgctcagttc
     2401 cgtcacgacc tgcacggaac ccatcgctga gttcacgtca gacggcaacc tctgttttac
     2461 cgacgaggtg aacctgggga gcgcgctggg caaagtgctt catcgccggg agatattcga
     2521 ggtgtccggt gatctgagga actacgaccc cgccggcata gggaatgcgt tgaggggaca
     2581 gcgaggcgtc ctgccggcgc ggaccgtgac ggaggtcctg gaaaaagcgg gctttgtttt
     2641 tccgccgcag gtggaggccg tatccctcga ggagatgaaa gaggcctgcg cgaggatagg
     2701 gtaccccctg gccatgaagg tcctcgggcc gctgcacaag agcgacctcg gcggtgtgaa
     2761 gctcggcatc gcggacgccg ccgcggccga acgggcctgg catgacctga cgggcatcga
     2821 ggacgcgcgc ggtgtcctcg tccaacccat ggtggaaggc acggaagtca tcctgggggc
     2881 gagccgtgcc ggtgagctgg gccacctcgt catgttcggg ctcggcggga tatataccga
     2941 ggtgctcagg gacgttacct ttgcccttgc ccctcttgcc ggggaggaat gccgcgggat
     3001 ggtgaaaggt attcgttctt acccgatcct ggaaggtgtg cggggtcaaa gggcagtctc
     3061 cgtggagcgt ctcgcggacc accttaagcg tcttggccgc ctcgtcttcg attttcccgt
     3121 catcggcgag atcgacatca accccctgaa gggcagcggc gacgaactgt atgtggtcga
     3181 cgcgcgtatt gtcctttatg ccgcgccgca ttcctgaaat gaaaaaggcc ggccccataa
     3241 taacgggaga ccgacctttt gtgtctgtcc tgcagtctta cagtttgaag agaaggtccg
     3301 tgtacgtcgg aacgggccac gtatcggcag ggacaagcat ctcgaggctg tcgatatccg
     3361 cgcgcagcgc ctggaccgtg gcgaagacct tgtcacggta gctctcggcc tgtttcacgg
     3421 tgtcgctgac agcctgcgcc ttttcgaccg cagcttccag tttgccgagg tttttgtacg
     3481 ccgaattgag aatggcgctg agctttttca gtatgtcttc cggaacagcg gcaggaccct
     3541 tgacggattt gaggtcgtct atggaatcgg cgaggaaggt cgcgtattcg agggccgcgg
     3601 gaatgaactg cttcctcacc atgtcgatgg cgacgcgcgc ctcgatattg atctgtttcg
     3661 cataggtctc gacgtagata tcgtagcggg aatggagctc cttgcgcgag aggacgtcgt
     3721 acttttcgaa gagcttgaag gccttgtccg tcacgaatgc cttgagcgcg tcgacggtgt
     3781 tggccacgtt cgggaggcct ctcttttcgg cttccgccac ccactcgtcg gagtagttgt
     3841 tcccgttgaa tatgatccgg ctgtgtttct tccaggtgtc ctttatgatc gcggcggcct
     3901 cggcattctt gtccctggcc ttttccagtc tcgtggcgat ctcgtcgagg gcctcggcgg
     3961 ctatggtgtt cagggccacg ttcgatcccg ctatggactg ggcggaacct accatcctga
     4021 actcgaactt gttaccggtg aaggcaaagg gcgaggtcct gttcctgtcg gtgttgtccc
     4081 tgggtattat cggcaatgta tcgacgccga cgccgacgaa ttgttcgccc ctcgatgtgg
     4141 tcttttc
//
LOCUS       DAOQTR010000141         4146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24086_length_4146_cov_3.825959, whole genome shotgun sequence.
ACCESSION   DAOQTR010000141 DAOQTR010000000
VERSION     DAOQTR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24086_length_4146_cov_3.825959"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKN85_04570"
     CDS             complement(<1..516)
                     /locus_tag="PKN85_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43709.1"
                     /translation="MKTKMMVLCALSFLAAACLTAGCGREESATFTTPEGKVTVTAKQ
                     SGAEGTVKVETKEGTATVTTGPQAVNEAELGAPAYPGAQVISSGQFGESKGTRAGSAS
                     AVLLSTNDSFDKVVAFYKANLKDIRQTMDQTAGDQKMVMFMAGREGNMRNVQIVAGAS
                     GGPTNIHIMKVT"
     gene            complement(628..1734)
                     /locus_tag="PKN85_04575"
     CDS             complement(628..1734)
                     /locus_tag="PKN85_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT43710.1"
                     /translation="MGETIRQGFCALPILMLCLVVSCCVTPTGFDEGAWRKKVEAARA
                     ADLYAPHYKDGKYFNPWMPMEEKGFLRFLKWKLSPSGEYTAEERRFLPSFIPGAAERI
                     EAMPEGDLILWVGHATFLIRLGSRFWLTDPILSDRALLPRRKISPAMTPGELKRLGSP
                     GLNVIISHNHYDHLDKQTLESLPDNTRFFVPLGMKEFLMNLGKKDVIEMDWWQSVDAG
                     DGTTVVCLPAQHWSRRIGQAVNSTLWASFLMISPKNTVYYGGDSGYFIGFREIGRRYP
                     GIDYALIPVTAYHPRWFMHYAHMDIDECIDAFDDLGARYFVPTQWGTFHLGDDPPGLP
                     ALDLKRRIAVRDLDPSRFLIMDIGEILPLSGKTE"
     gene            complement(2589..3221)
                     /locus_tag="PKN85_04580"
     CDS             complement(2589..3221)
                     /locus_tag="PKN85_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT43711.1"
                     /translation="MLRVIIADDHPVVLKGLREILQENFSGMTFDEARKGYELINKIH
                     QNDYNLVLLDISLPDINGLEVLKEIKKRRTRLPVLIVSMYPEEQYAIRSIKAGAQGYI
                     TKRSASEELVAAVNKILSGKRYINPTFAEQMVIDFESDTEAPPHEKLSIREMQVMKMV
                     ASGLTNKEIAEQLHLSINTVRTYRVRILEKISVKKTNELIWYAVKYGLVE"
     gene            complement(3185..4096)
                     /locus_tag="PKN85_04585"
     CDS             complement(3185..4096)
                     /locus_tag="PKN85_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNT43712.1"
                     /translation="MDSPGTAGKKTTRREAPSPSRRPDTQEVLEQRVQQRTAQLSELN
                     ALLRQEIAERRKMETALQDSRERLRHLSIHLQKIRENERTSLSRELHDEFGQTLTGMK
                     LDVSWIKRRVASDDGLVVERLDSVLADLDRTIIAVQQMSARLRPVALDDFGLRDAVEL
                     AARDVTKRTNIACHIISEPYNITLNDTVTTGAFRILQEALTNVVRHSGAREVTILLRK
                     RKDVFTMEISDDGKGIRKKELVNPRSIGLTGMHERAHALGGTLAIMGVRGKGTSITLT
                     VPLRPVRRDNKRGMDKCSESSSQTTTP"
BASE COUNT          798 a         1192 c         1149 g         1007 t
ORIGIN      
        1 cgtcaccttc attatgtgta tgttcgtcgg tccaccggag gctccggcga cgatctggac
       61 gtttctcatg ttgccttccc tgcctgccat gaacatgacc atcttctggt ctcccgccgt
      121 ctgatccatt gtttgccgga tgtccttcaa gttggccttg tagaacgcga cgaccttatc
      181 gaaggaatca ttcgtgctga gcaggacggc tgaggccgat ccggcccggg tgccctttga
      241 ctcgccgaac tgccccgaag agatcacctg tgcgccggga taggcgggtg cgccgagctc
      301 agcttcgttg accgcctgtg gacctgtcgt gacggtcgcg gtgccttcct tcgtctccac
      361 cttcaccgtg ccttccgcac ctgactgttt cgccgtcacc gtcaccttgc cttccggggt
      421 cgtaaaggtt gcagattcct ccctgccgca acctgccgtc aggcatgccg cggcaagaaa
      481 ggacagagca cacaaaacca tcatcttcgt cttcatactg tcctccctgt catccttagg
      541 tattgtccgg gtatgtgcct tgaagccatt atagtacagg gagcaaatga ttacaacaaa
      601 gggggagggg cgatacgggg ccgaagatca ttctgtcttt cccgacagcg gcaatatttc
      661 gccgatatcc atgatgagaa atctcgatgg atcgagatcc ctgacggcga tccttctctt
      721 gaggtcgagg gcgggaaggc cgggaggatc gtcaccgagg tggaaggtgc cccactgagt
      781 gggcacgaaa taccgggctc ccagatcgtc aaacgcgtcg atgcattcgt cgatatccat
      841 gtgagcgtag tgcataaacc agcgcggatg gtaggcggtt accggtatca gggcgtagtc
      901 tatcccggga tagcgcctgc ctatctcgcg gaaacctatg aaatatccgc tgtccccccc
      961 gtaatagacg gtgttcttcg gagagatcat gagaaaactt gcccagagtg tgctgttcac
     1021 ggcctggccg atccggcggg accagtgctg ggccggcagg cagaccaccg tcgtgccgtc
     1081 accggcatcg acgctttgcc accagtccat ttcgatcaca tccttctttc cgagattcat
     1141 caagaactcc ttcatgccca gggggacgaa gaagcgggtg ttgtccggca gcgactcgag
     1201 ggtctgcttg tccagatgat cgtagtgatt gtgagatatg atcacgttga gccctggcga
     1261 gccgaggcgc ttgagctccc cgggcgtcat cgcggggctg atctttcttc ggggcaggag
     1321 cgccctgtcg gacagtatgg ggtcggtcag ccagaaccgg gagccgaggc gaatgagaaa
     1381 ggtcgcgtgg ccgacccaga gaataaggtc gccttcgggc attgcctcga tgcgttccgc
     1441 ggctccggga atgaaagagg gcaggaacct cctctcttca gcggtgtact cccctgaggg
     1501 tgagagcttc catttcagga aacgcagaaa gcctttttcc tccataggca tccatgggtt
     1561 gaagtatttc ccgtccttgt aatgcggcgc gtagaggtcg gcagcgcgcg ccgcctccac
     1621 cttttttctc cacgccccct catcgaaccc ggtgggtgtc acgcagcagg agacaacgag
     1681 gcacaacatc aggatcggaa gagcacaaaa cccctgtctg atcgtttccc ccatggtcac
     1741 tccactccac agtgtagcac aggaggcggg agacagggaa cagggacaga gaggaccaat
     1801 aggatgtgga taggaccaat aggaccaatt ggacatatat gacgggatag gacctattgg
     1861 acggatcttc aatttgcacg acaggaacgg catcctgttc ctgaaacccg aaaccgcctt
     1921 tatctgcttg aaccttggac tttcttctat acccacacga cgcaattgaa gatgccgcgt
     1981 tttgctgctc tccgggtcgt gggaccggtt tcagcacggc cggtgccgct cacgattata
     2041 cgatattcgt ggacgcccgg gctgagtctc agcgttgtcc tccacgtccc atccttacat
     2101 ttcctgagcg gccttgtggt ctctgtcccg tcacggatac tccctgccat gaacacctcg
     2161 tgagccgatg gctcatgcag tttgaatgtg accttctttt tgtcccgtct gtcggccatt
     2221 tcctcaacgc accgtgaaaa ggtcaagtat gaagcccggg gaaatactcc acgtccagga
     2281 tccctctaat agagactcta caataggaat cccccccctg tctgtcgccg aatcgacatt
     2341 tatcttgtgg tagtttcgtt caaaagagct gtcatcgttt cctatccgat gacctcccga
     2401 gattgttcct gcgggatctt gcggcggatc gatcgtctct tatgtattca aacggttcga
     2461 gacgttcaac agactgtcga taaaatcatt ttctccgtca ttccaacttt agaccggaat
     2521 gacggaggga cacgaaccgc ctttggaaat gactttgtca gcagcctgtc aagaggttca
     2581 agtcgtaatc actcgacgag gccgtatttc accgcgtacc agatcagctc attggtcttc
     2641 ttgacggaga tcttttccag gatacgcacc cggtaggtcc tgacggtatt tatgctgaga
     2701 tgaagctgct cggctatctc cttgttcgtc agtccgctgg cgaccatctt catcacctgc
     2761 atctcccgga tggaaagttt ctcgtgaggc ggggcctccg tatcggactc gaaatcgatg
     2821 accatctgct ccgcgaaggt ggggttgatg tatctcttgc ccgacagtat cttgtttacc
     2881 gcggccacca gttcctccga cgcgctgcgc tttgttatgt aaccctgggc gccggccttt
     2941 atggagcgga tcgcgtactg ttcctcggga tacatgctca cgatgaggac gggcagacgt
     3001 gtccttctct tttttatctc cttgaggact tcgagaccgt ttatgtcggg aagagagatg
     3061 tcgagaagca ccaggttgta atcgttctga tggatcttat tgatcagctc gtagcctttc
     3121 cgcgcctcat cgaaggtcat gccgctgaaa ttctcctgca agatctccct gaggcccttg
     3181 agcactacgg ggtggtcgtc tgcgatgatg actcggagca tttgtccatt cctctcttgt
     3241 tgtcgcgcct gaccggacgg agcggaactg taagagtgat cgaggtgcct ttaccccgca
     3301 cacccatgat cgcaagcgtt ccacccagtg catgcgcccg ttcgtgcatg cccgtcaaac
     3361 cgatagacct ggggttcacc agttccttct tcctgatgcc cttgccgtca tcgcttatct
     3421 ccatagtaaa gacatccttg cgctttctga ggagtatcgt gacctcacgc gccccggaat
     3481 ggcgcacgac gtttgtaagg gcctcctgga ggatccggaa cgccccggtg gtcacggtgt
     3541 cattgagggt gatgttgtag ggttcggaga tgatgtgaca tgcgatgttc gttcttttcg
     3601 tgacatccct ggcggcaagt tccactgcgt cccggaggcc gaaatcgtcg agagcgacgg
     3661 gccggagtct ggctgacatc tgctggacgg caatgatggt gcggtcaaga tcggccagca
     3721 cggaatcgag ccgttccacg acaagtccgt cgtcggaggc gacccttctc tttatccagc
     3781 tgacgtcaag cttcatgcct gtcagggttt gaccgaattc gtcatggagc tctcttgaga
     3841 gtgaggtacg ctcgttttcc cttatcttct ggagatgtat gcttaagtga cgtagccgtt
     3901 ccctggagtc ctgcagggcg gtctccatct tcctgcgctc cgcgatctcc tgtctgagga
     3961 gagcgttcaa ttccgacagc tgtgccgtcc gctgctgcac gcgctgctcc aggacttcct
     4021 gggtgtccgg tcggcgggat ggtgacggag cctcccgccg tgtcgttttc tttcctgccg
     4081 ttccgggact atccactgct cccccttgcg atcatccttg gcggaccgtg cgctcacgcg
     4141 gccggt
//
LOCUS       DAOQTR010000142         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24172_length_4134_cov_2.907330, whole genome shotgun sequence.
ACCESSION   DAOQTR010000142 DAOQTR010000000
VERSION     DAOQTR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24172_length_4134_cov_2.907330"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PKN85_04590"
     CDS             complement(<1..305)
                     /locus_tag="PKN85_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT43713.1"
                     /translation="MDTFKEMDRRDAMRFICVLFVSVLMPHSAESKEIGKLLENEDRE
                     GFYIRFIKPMRPVDPATWVLKVGGLCERPRNLDLAALKGLPKVTQVSRMKCVESWSS"
     gene            385..1146
                     /locus_tag="PKN85_04595"
     CDS             385..1146
                     /locus_tag="PKN85_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT43714.1"
                     /translation="MLIIEDLQVELAGKMLLKDIDLEIKPGETHVLFGPNGSGKTSLL
                     MTIMGYPQYVVKKGKITFKGRDITHAPLNERAALGIGMSYQRPPTINGLKTRQMIDIC
                     ARRKVDAEEMARSLNFEEFLDRDVNAGFSGGEIKRSELLQLKAQDPDLMLFDEPESGV
                     DIENMALIGTTISQILEKDTPRAGTNVKPFVQQRSERTKMGFIITHTGYILDYVPADK
                     GQVLYNGILACAGNPREIFQCIGRLGYEECVKCTV"
     gene            1134..2360
                     /locus_tag="PKN85_04600"
     CDS             1134..2360
                     /locus_tag="PKN85_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNT43715.1"
                     /translation="MYSLEELEQRARQAVGKKAAFGDDVDLDSFDKTFVPHKYLAEEE
                     ICAIPEEEQQRLIMSGLDVTDTGRGGTYFQKDTTVVHCHSDEEGIEVMPVTEALKKHD
                     WVKEYFWKLVSVDQDKYTASAFLDLHDGYVIRALPGSKSIYPIQACLYLDKENLQQNV
                     HNLIIAEEGSELHIITGCATSPHMKRGVHVGISEFFVKKGAKLSFTMIHNWAEEMSVR
                     PRSVARVEEGGVFINNYICMKPVKSIQMYPTTHLVGDNATAMFYSVIVGTSGSYLDIG
                     GRIYLKNRGCRAEIVQRSISNGAHIINRGHLIGEAPDIKAHLECKGLLLSGGIIDSIP
                     QLEGHVEGVDMSHEAAVGKVAQEEINYLMSRGITEEEATSTIVRGFLSVDIKGLPTPL
                     KQEIDRAIDRSNKDAM"
     gene            2373..3260
                     /locus_tag="PKN85_04605"
     CDS             2373..3260
                     /locus_tag="PKN85_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT43716.1"
                     /translation="MKRRDFLKLGVSALVASQLPRNLFGQAGQPLVSVCTGTDYAAVT
                     RKVITALGGMRAFVKPGNTVVVKPNMGWDRKPEYAANTHPLVVKTIVEECLASGARKV
                     KVFDNTCTDARRSYENCGIPAALKGMKNVDVRFVENDRFRKTALNGRFLKDWPLYDDA
                     LKADVLINVPIAKHHGLTGLTLGLKNMMGIMGDNRGYIHRGIEDALSDVNSVVKSHLV
                     VIDATRILTDHGPQGGDLKDVKVLNTVIASRDIVAADAYATTLFGMKPQDISTTVTAY
                     KRGLGEMDVKKMRVLSFRF"
     gene            3459..>4134
                     /locus_tag="PKN85_04610"
     CDS             3459..>4134
                     /locus_tag="PKN85_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT43717.1"
                     /translation="MRLPASRISQLLFLALFLFLFIITDYRGKDEITVAVNSFFRANP
                     LVLASYVLAVKGFTFLLLPALLLTIFTVVLGRFFCGWICPLGTLIDLVTQRIPKSTPI
                     RFLKGSFKYYLLFTLLVTALFSVNISGILDPIAILIRALTLVFYPLVGYVARAGWSGL
                     YQVAGEGRDNVAFLYDFLRYYILPFRETFYPLAFLSLAIFLFVLILERFERRNWCRNI
                     CPLGTLL"
BASE COUNT          973 a         1198 c         1116 g          847 t
ORIGIN      
        1 ttgctcgacc atgattcgac gcacttcatg cgcgacacct gtgtcacctt cggaagtccc
       61 ttgagggccg cgagatccag gttccgcggt ctctcgcaga gcccgcccac cttgaggacc
      121 cacgtagccg ggtctacagg ccgcatgggc ttaatgaacc tgatatagaa gccctcgcgg
      181 tcctcgtttt cgaggagttt ccctatctct ttcgactccg ccgaatgggg catgagaacg
      241 gatacgaaaa ggacgcaaat gaagcgcatc gcgtcccgtc tgtccatttc cttgaaggtg
      301 tccatatcac aagtatagcc ttgacccttg acaaacagaa gcggataatt agattcagga
      361 acaattagaa taaaggagtg ggccatgttg ataatcgaag acctgcaggt ggagttggcg
      421 ggaaagatgc tcctcaagga catcgacctt gagatcaagc cgggggagac gcatgtcctt
      481 tttggtccca acgggtccgg caagacatcc cttctcatga ccatcatggg ctatccgcag
      541 tacgtcgtca agaaagggaa gataaccttc aaggggaggg acataacaca cgcccccctc
      601 aacgagcgtg ccgctctcgg gatcggtatg tcctatcagc ggcctcccac gataaacggc
      661 ctcaagacga ggcagatgat cgacatctgt gccagaagaa aggtcgatgc cgaggaaatg
      721 gcgcggtccc tcaactttga ggaattcctc gacagggacg tcaacgccgg tttctcgggc
      781 ggggagatca agcgctcgga actccttcaa ctcaaggccc aggaccctga ccttatgctc
      841 ttcgatgaac ccgaatcagg cgtggacatc gagaacatgg ccctcatcgg caccacgatc
      901 tctcaaatcc tggaaaagga cactccgaga gcgggcacga atgtaaagcc gtttgttcag
      961 caacggagtg aacggaccaa gatgggcttc atcatcaccc atacgggcta tatcctggat
     1021 tacgttcccg ccgataaggg ccaggtcctc tacaacggaa tcctggcctg cgcgggcaat
     1081 ccccgcgaga tatttcagtg catcggacga ttaggctatg aggagtgtgt gaaatgtaca
     1141 gtctagaaga gcttgaacag agggcccgtc aggcggtcgg taagaaagcc gccttcgggg
     1201 atgacgtcga cctcgactcc ttcgacaaga cctttgtgcc ccacaagtac ctggccgagg
     1261 aggagatatg cgctatcccc gaggaggaac agcagcgtct cataatgtcg gggctcgacg
     1321 tgaccgacac cggaaggggc ggaacatatt tccagaagga caccaccgtc gtccattgtc
     1381 atagtgacga agaaggcatt gaggtcatgc ccgtcacgga ggcattgaag aaacacgact
     1441 gggtcaagga gtatttctgg aagctcgttt ccgtggacca ggacaagtat acggcgtcgg
     1501 ccttcctgga cctccacgat gggtacgtca tcagggccct gcccggaagc aagagcatct
     1561 atcccatcca ggcctgcctt tacctcgaca aggagaacct ccagcagaat gtccataacc
     1621 tcatcatcgc cgaggaggga tcggagctgc acatcatcac cggctgcgcg acgtcgcccc
     1681 acatgaaacg cggcgtccat gtgggcatat cggaattctt cgtcaagaag ggcgccaaac
     1741 tgagctttac catgatccac aactgggccg aggaaatgag cgtccgtccc cgctccgtcg
     1801 cccgcgtcga ggaaggcggc gtcttcatca acaactacat ctgcatgaag cccgtcaaat
     1861 ccatccagat gtatcccacg acgcacctcg tcggcgacaa cgccacggcc atgttctaca
     1921 gcgtcatcgt cgggacctcg ggctcctacc tcgatatcgg cggaaggatc tacctcaaga
     1981 acaggggatg ccgggccgag atagtgcaga ggtccatcag caacggcgcg cacatcataa
     2041 accgcggcca cctcatcggg gaagcccccg atatcaaggc acacctggag tgcaagggac
     2101 tcctcctgag cggcggcatc atagattcga taccacagct ggagggccac gtcgaaggcg
     2161 tcgacatgtc acacgaggcc gccgtgggaa aggttgccca ggaggagata aactacctca
     2221 tgtcgcgggg gataacagaa gaagaggcaa catcgaccat cgttcgcggc tttctgagcg
     2281 tggacatcaa gggccttccc acaccgctga agcaagagat agaccgcgcc atcgacagga
     2341 gcaacaagga tgcgatgtga ggagggccac ccgtgaagcg cagggacttt ctgaaactcg
     2401 gagtttcggc ccttgtggcc agccagcttc cacgaaatct cttcggacag gccgggcaac
     2461 ccctcgtctc ggtctgtaca ggcacggact atgccgctgt cacccggaag gtgataaccg
     2521 cgctcggggg catgagggcc tttgtgaagc cgggaaacac cgttgtggtc aaacccaaca
     2581 tgggctggga cagaaaaccc gaatatgccg cgaacaccca ccccctcgtg gtgaagacca
     2641 tcgttgagga atgcctcgct tcgggcgcca gaaaggtgaa ggtcttcgac aacacctgca
     2701 ccgatgcgcg ccgctcttac gagaactgcg gcatcccggc agccttgaaa ggcatgaaga
     2761 acgtcgatgt ccggttcgtc gagaacgacc gcttcaggaa aacggccctc aacgggcgtt
     2821 ttttgaaaga ttggcccctg tatgacgatg cccttaaggc cgacgtgctc atcaatgtac
     2881 ccatcgccaa gcatcacggc ctgacgggcc tgaccctggg tctcaagaac atgatgggga
     2941 tcatggggga caacagggga tacattcacc gcgggataga agacgccctg tccgacgtca
     3001 acagcgtcgt gaaaagtcac cttgtagtca tcgatgcgac acggatcctc accgatcacg
     3061 gcccccaggg aggggatctc aaagacgtga aggtcctcaa cacggtgatc gcctcgcggg
     3121 acatcgtggc cgcggacgcg tatgccacca ccctcttcgg aatgaaaccc caggacatct
     3181 ccacgacggt cacggcatac aagcgcggac tgggggagat ggatgtgaag aagatgaggg
     3241 ttttaagttt taggttttaa gttttacgtg aaggacgtga ggacgagaat agctgtcccg
     3301 ttgtcggcaa ttttcttccc cgatctcggc ttagaacaca aagcctgaaa cctagaactt
     3361 ttaataggcc acatatatga tacataaaga gacccaaatg tttacgacgt acacaaatga
     3421 ggtttctaac ttaaaacgta aaacgtaaaa cggtctttat gagactcccc gcctcccgta
     3481 tctcccagct tcttttcctc gccctctttc tcttcctttt catcatcacc gactatcggg
     3541 gaaaggatga gataaccgtt gccgtcaata gcttcttcag agccaatccc ctcgtgcttg
     3601 caagctacgt cctcgccgtc aagggtttta cgttccttct cctgcccgcc ctgttgctga
     3661 cgatcttcac cgtggtcctg ggaaggttct tttgcggctg gatatgtccc ttggggacct
     3721 tgatcgatct tgtcacgcag aggatcccga agagcacccc catccgtttc ctgaagggct
     3781 cattcaaata ctatcttctt ttcacgcttc tggtaacggc cctcttttcc gtgaacatct
     3841 cgggcattct tgatcccatc gcgatcctca tcagggcgct taccctcgtc ttctaccccc
     3901 tcgtcggcta tgtggccagg gcgggctggt cgggcctcta ccaggttgcc ggagaaggac
     3961 gggataacgt ggcgtttctc tatgatttcc tcaggtacta tatcctgcct tttcgagaga
     4021 ccttctaccc cctggcgttc ctctccctcg ccatcttcct tttcgtcctc atccttgaac
     4081 ggtttgaacg gaggaactgg tgcaggaaca tctgcccgct cggcacgctt ctcg
//
LOCUS       DAOQTR010000143         4084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24596_length_4084_cov_3.901713, whole genome shotgun sequence.
ACCESSION   DAOQTR010000143 DAOQTR010000000
VERSION     DAOQTR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24596_length_4084_cov_3.901713"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKN85_04615"
     CDS             complement(<1..217)
                     /locus_tag="PKN85_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNT43718.1"
                     /translation="MKNGRSEEKGGALFSAEEMERYSRQMMIPSIGQEGQERLRKAKV
                     CIAGAGGLGSVSAYYLAAAGVGRIRIVD"
     gene            complement(228..1142)
                     /locus_tag="PKN85_04620"
     CDS             complement(228..1142)
                     /locus_tag="PKN85_04620"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNT43719.1"
                     /translation="MSSVPEFPFFKPVELPDKPVIDGILRGYRPLTSELTFTNLFIWR
                     KHFDFRWSVHRDWLFIAGREDVCPRFAVGPIGPPGRAEAARLLLEWLREDAGDSEPCI
                     ERADESLALEVSGEPGFLVEETRDHFDYVYLTRDLIDLAGGAYRAKRNHINQFHRAVS
                     SYLYEELEERHIEECLALQERWCLLRRCEEDLNLQGEWEATKEILMHHRSLGVSGAVI
                     VIDSGVKGFTLGESLGGDMAVIHIEKADPEIPGLYQFINQQFCARRWSGVKFVNREQD
                     LGIRGLRDAKLSYGPDHFVKKYRIMLKG"
     gene            1325..2278
                     /locus_tag="PKN85_04625"
     CDS             1325..2278
                     /locus_tag="PKN85_04625"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT43720.1"
                     /translation="MDSKDLAIGLDVNKIRHITDSLSTYLQGKEKPLALSLVSFFSRG
                     HVLIEDLPGLGKTTLAIGIAKSLGLTFGRIQCTSDLLPTDITGLSIYNKTSGEFEFHP
                     GPIFNNIVLCDEINRATPKTQSALLEAMEEKQVTIEGKTYKLPRLFCVIATQNPTEQF
                     GTFPLPESQLDRFMMKISIGYPSRQAEKEILKIGSKREDIYDIEPVVNQDEVSRIQEE
                     ISRNIHVSDKILEYTLSIIHETRTSEYLTAGLSTRGALTIVNTARANAFFHDRHFVIP
                     EDIRDLVEYTIPHRVIFKEEYENLNKREIVRSIVDAVPTPL"
     gene            2395..3150
                     /locus_tag="PKN85_04630"
     CDS             2395..3150
                     /locus_tag="PKN85_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT43721.1"
                     /translation="MSISGVFGKTNLSKLDLAIEFPQEVYAGSRVPVKITMQNRRRFL
                     PAFLIGLSAGRISALLPFTPSKGTSSVYTSVTFHERGLHTIENPGVHSVFPFNFFTRF
                     KRLHRTYTITVLPRLKACDLASLYRKERRSRGEKTSDTIGYDSDIVSIREYVRGDPIK
                     YIHWKATAKTGKLKTKEHSSLSYQPVIIEFDRVLIRDIEERISCVAYTIVQLLRNSVP
                     VGLRIDGRLYPPDVTGVHKLNMLRELAVYEKHG"
     gene            3241..>4084
                     /locus_tag="PKN85_04635"
     CDS             3241..>4084
                     /locus_tag="PKN85_04635"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HNT43722.1"
                     /translation="MVDVFTYIIGVLCIVAVSGHIPFVYPLVLFGAMGLSVYLDLKKV
                     SIPRWILTVVSVAVILYFLIRIDVQDLIGQIMEALFILMGIKYLEHREVRDFMQIYAL
                     ALFVLAGLGLMTLGMAFVAYLLVFFLLLSLSFIFLTFYSQDPELELTNSTVRTMISRC
                     LWIPLLAIPLSAAMFVILPRTSYPILSFLNRPDKARAGFTDNVRLGQVSDIQEDERII
                     FRATMDRVDESSLYWRGITLDHFDGTSWRNARRGVYTPAGTRGPVGAMISQTIYLEPY
                     DNTSL"
BASE COUNT          887 a         1278 c         1061 g          858 t
ORIGIN      
        1 gatcgacgat ccggatccgc cccaccccgg cggcggcgag gtagtatgcc gagaccgacc
       61 ccaaccctcc ggcaccggcg atgcagacct tcgccttcct gagacgttcc tgaccctcct
      121 gtccgatcga ggggatcatc atctgccggg aataccgctc catctcctcg gcggaaaaaa
      181 gggccccgcc cttttcctct gatcttccgt tcttcattct ccacccgtca tcctttcaac
      241 attatcctgt acttcttgac gaaatggtcg ggaccgtagg aaagcttcgc gtcccggagc
      301 ccccgtatcc cgaggtcctg ttcccggttg acgaacttca cgccgctcca gcgcctcgcg
      361 cagaactgct gattgatgaa ctgatagagg ccgggtatct caggatcggc cttttctata
      421 tggatcaccg ccatgtcacc tcccagggac tcaccgagtg taaagccctt gaccccggag
      481 tcgatgacga tgacggcccc cgacacaccc agggaacggt ggtgcataag gatctctttc
      541 gtcgcctccc attcgccctg gagattgagg tcctcttcgc acctcctgag gagacaccac
      601 ctttcctgga gcgcgaggca ctcctcaatg tgtcgctctt caagctcttc atacaggtat
      661 gaggagacag cgcggtggaa ctggttgatg tggttccgct tggcccggta cgcaccgccc
      721 gccagatcta tgaggtcccg cgtcaggtag acatagtcga agtggtcccg cgtctcttcc
      781 accagaaacc cgggctcgcc cgatacctca agggcaaggc tctcatcggc gcgctctata
      841 cagggctcgg aatccccggc atcctccctc aaccattcaa ggagaagtct tgcggcctct
      901 gcccggcccg gcgggccgat gggtcccacg gcaaacctcg ggcatacatc ctccctgccg
      961 gcgatgaaga gccaatccct gtggaccgac cagcggaagt cgaagtgctt cctccatata
     1021 aagaggttcg taaaggtaag ttccgacgtc aagggccggt acccgcggag aatgccgtca
     1081 atgaccggct tgtccgggag ttcgacgggc ttgaaaaagg ggaactcagg aacggaagac
     1141 atgcctctat tcaagcggaa ttacggtggt ttgtcaagag aggtgggcag aggcagggga
     1201 aaaagggggc agaagaagga accttcacga agaaatagga cgaataggat ataggaccta
     1261 taggtcctat cttccccgcc cctccttttc ccctttcccc ttgttttgtg ttacaatgac
     1321 gtgcatggac agcaaagatc tcgccatcgg gctcgacgtc aacaagatcc ggcacatcac
     1381 cgactcgctt tcgacgtatc tgcaaggcaa ggagaaacct ctcgccctgt cccttgtctc
     1441 cttcttctca cggggacacg tactcattga ggaccttccg ggcctgggga agaccactct
     1501 cgccatcggg atcgcgaagt ccctcggact aacgttcggc cgcatccagt gcacgagcga
     1561 cctccttccc acggacatca ccgggctgtc catatacaac aagacgtcgg gagagttcga
     1621 gttccatccc ggtcccatct tcaacaatat tgtcctctgt gacgagatca accgtgccac
     1681 cccaaagacg cagagcgcgc tcctcgaggc catggaggaa aaacaggtaa ccatcgaggg
     1741 caagacctac aagcttcccc ggcttttctg tgtcatagcc acgcagaacc cgacggagca
     1801 gtttggcacc ttccccctcc ccgaatcgca gcttgaccgt ttcatgatga agatcagcat
     1861 cggctatccg tcgcggcagg ccgaaaagga gatcctcaag atcggcagca agagagagga
     1921 catatacgat atcgaacccg tcgtaaacca ggacgaggtc tcccgcatcc aggaggagat
     1981 ctctcgcaac attcatgttt ccgacaagat cctcgagtac accctgtcca tcatccatga
     2041 gacgagaacg agcgagtacc tcacggcagg tctgtccacg cggggtgcgc tgacgatcgt
     2101 caataccgcg cgggccaacg ccttcttcca cgacaggcac ttcgtcatac ccgaggatat
     2161 ccgcgacctc gtcgagtaca ccatccctca ccgcgtcatc ttcaaggaag agtatgagaa
     2221 cctcaacaaa agggagattg taagatcgat cgtagacgcc gttcccacac ccctgtaata
     2281 aagatatcca aggcaggctt catctacata gcgctcacca tctttctcgg catcgcggcg
     2341 gcgaatacca acaataatct catctttctc gttgtctccg ccctcttgag cttcatgagc
     2401 atatcggggg tcttcgggaa gacgaacctc tcgaagctgg atctcgcgat agagttcccc
     2461 caggaggtgt acgcgggaag ccgggtcccc gtgaagataa cgatgcagaa ccgccgcagg
     2521 ttcctccctg cctttctcat aggcttgagc gccggccgga tctcggccct tctccccttc
     2581 acgccgagca aagggacctc ttcggtctac accagtgtga cctttcatga acggggcctt
     2641 cacaccatag agaaccccgg cgtgcactcc gtgttccctt tcaatttctt cacccggttc
     2701 aagagactgc accggaccta tacgataacg gtccttccga gactgaaggc ctgcgatctg
     2761 gcaagcctgt accgcaagga gcgcagatcg agaggggaaa agacgtccga cacgataggc
     2821 tacgattccg atatcgtgtc catcagggaa tatgtccggg gagaccccat caagtacatc
     2881 cactggaagg cgacggccaa gacaggcaaa ctcaagacga aggagcattc gtcgctgtcc
     2941 tatcagcccg tcatcatcga attcgacagg gtcctcatca gggacatcga ggaacgcatc
     3001 tcctgcgtcg cctacaccat cgtccagctc ctcaggaaca gcgtgcccgt tgggctcagg
     3061 atagacggtc gcctctaccc cccggacgtg acgggggtcc acaagctaaa catgctgcgg
     3121 gaactggcgg tgtatgagaa gcatgggtga tgggtgatga gttataggtg aggggggcag
     3181 actgaggttc gctgaagata tatgatcacg atccgtttca cacaaatgaa agtccgcacc
     3241 gtggttgatg ttttcacgta catcatcggg gtgctttgca tagttgccgt gtccggccac
     3301 attccctttg tctatcctct cgtgcttttc ggcgccatgg gcctctcggt ctacctcgac
     3361 ctgaaaaagg tctccattcc ccgctggatc ctcaccgtcg tttcggtggc cgtcatcctc
     3421 tactttctca ttcgcatcga cgtgcaggac ctgatagggc agatcatgga ggcccttttc
     3481 attctcatgg gaataaagta cctggaacac agagaggtcc gtgattttat gcagatatac
     3541 gccctcgccc tcttcgtcct cgcagggctc ggcctcatga ccctggggat ggccttcgtg
     3601 gcgtacctgc ttgtcttttt tctcctcctg tccctgtcgt tcatcttcct caccttctat
     3661 tcacaggacc cggaactgga gctcacgaac agcaccgtgc ggacgatgat ctcgcgatgc
     3721 ctgtggatac cgctgctcgc catccccctt tccgccgcga tgttcgtcat ccttccccgc
     3781 accagttacc ccatcctctc ttttctcaac agacccgaca aggcaagggc agggttcacg
     3841 gacaacgtca ggctcggcca ggtgtccgat atccaggagg atgaaaggat cattttcagg
     3901 gccaccatgg acagagtgga tgaatcaagc ctctactgga ggggcatcac cctcgaccac
     3961 ttcgacggga cgtcctggag gaacgccagg cgtggcgtct acacgccggc cgggacccgg
     4021 gggcccgtcg gcgccatgat aagccagacg atctatctcg agccttacga caatacctcc
     4081 ctct
//
LOCUS       DAOQTR010000144         4079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24648_length_4079_cov_3.721421, whole genome shotgun sequence.
ACCESSION   DAOQTR010000144 DAOQTR010000000
VERSION     DAOQTR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24648_length_4079_cov_3.721421"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKN85_04640"
     CDS             complement(<1..447)
                     /locus_tag="PKN85_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNT43723.1"
                     /translation="MNKTFAARSAMLLCISLLFLVPSCAPKKVQIFDMPAGARTDIIQ
                     IALAQQGKKYRSGAKGPDYFDCSGLIYFTYRQAGISVPLTAEAQGRYGAEISRGEAQP
                     GDLVLFRIRNDDHIGIMVNGSDFVHASKSRGVAIDSLESTYWRRYLI"
     gene            complement(447..1085)
                     /locus_tag="PKN85_04645"
     CDS             complement(447..1085)
                     /locus_tag="PKN85_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNT43724.1"
                     /translation="MAIRIVFLDCDGTLTTVKSSWEYLHRRLGLWSDNADEYQALFRQ
                     GAIDYHEFCRRDALLWRGLSVGDVEAVIGEISYQPGARDLIRSLKVAGMVTVIVSTGL
                     SLLVEKVRRELSVDLAFSNDLLSRDGTLSGEISIHVDYDLKGPIVRRTLERLGLSPAE
                     ASAIGDGEGDRGMFEEVGLPIGFNMNGSAPAFPERTIYIEELREAKSLLGVA"
     gene            1175..1756
                     /locus_tag="PKN85_04650"
     CDS             1175..1756
                     /locus_tag="PKN85_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT43725.1"
                     /translation="MSEEKEVREAADWDERYRKGFYDGPDGPHDLLVRYRHLFGGRVA
                     DIAMGSGRDALFLASAGIAVTGLERSWEGLRIAGQAAAARGLSIAMVQGDAARLPFRK
                     DSFQGVIVFYFLLREIAPDIAAVLDRGGILMYETFLKAGGDVDHPRNPAFLLDEGELP
                     ALFPGFDLIHYDEGLREHKGRMRATAQLVARKR"
     gene            1753..2367
                     /locus_tag="PKN85_04655"
     CDS             1753..2367
                     /locus_tag="PKN85_04655"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNT43726.1"
                     /translation="MIVEWNPERPKKKATELIVRTMQEGGIIAYPTDTHYGIGCDLFN
                     IRSIRKLYAMKRLDPKRALSIICRDMKDISTYAVLSDFSFDIVKWYLPGPFTFVLKAR
                     KIIPKLLMTDRKEVGVRIPAHAVPVGIAVLCGRPVINTSARIAGEEVMTDPRVIEKTF
                     GDNISLVVDGGILVSEPSTVVRLADDVAELVREGKGALDDRISR"
     gene            2378..3157
                     /locus_tag="PKN85_04660"
     CDS             2378..3157
                     /locus_tag="PKN85_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT43727.1"
                     /translation="MTERDVQEGSAGIIGNYRNHDLHARVGAIIREHSENRKDIRSVV
                     RDAIPWPRVESVLDLGCGYGWFEEVMPGGLDLIVGIDCLPENEAPFLAAARARAREAV
                     FRAMTLPAPTGLPADRFDLALSLYSLYFFPGAVAEAARVLKPGGLFLCLTHSSSMLEE
                     GQHYFDFRNLRKVIERFSAENGEALLKRHFTTVTCIDYPNALVFREGDEDSLALYIDF
                     KREFIEKDVDPRVVRDTMLSELRRKGELSFNKNDRIFVAGK"
     gene            3154..>4079
                     /locus_tag="PKN85_04665"
     CDS             3154..>4079
                     /locus_tag="PKN85_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT43728.1"
                     /translation="MKRRIYCAHCGTPLGTDFLEGKHRQVCGACDTVYYENPLPVASV
                     ILANGKREILLVRRAKEPFKDMWCCPIGFAETGESIEDAALRELKEEAGVEGRIVQLL
                     DVGSHVNPLYGELLIVTFEVEKTGGQERAGDDASEARYFPVMNLPKLAFDSQVKALAR
                     FVELKKDLWSIHDSFETFVESTVKDRIGYPVVLLSDELANAVQENSEKIVNLWLDDIT
                     TNPSTRSYRRVSREALRSRAMVIMGQFGAWLRKEKSEGEFTSFYRSLGRERNEGGVPL
                     EEAISSLSLLKKHVWMFTYSFGVWEKAVDIYR"
BASE COUNT          821 a         1162 c         1328 g          768 t
ORIGIN      
        1 tatcaggtat cttcgccagt aggtggattc cagggagtcg atggccacac cgcgtgattt
       61 ggaggcatgg acaaaatcgg agccgttgac catgatcccg atgtggtcgt cgttcctgat
      121 cctgaagaga acgaggtcgc ccggttgcgc ctctccccgg gatatctcgg cgccgtagcg
      181 tccctgcgcc tcggctgtga gtgggacgga gatgcctgcc tgcctgtacg tgaaatagat
      241 gaggccgctg cagtcgaagt aatcgggccc cttcgcaccg cttcggtatt tcttgccctg
      301 ctgagccagg gcgatctgga tgatatccgt gcgggcgcct gcgggcatat cgaatatctg
      361 caccttcttc ggggcgcagg aggggacaag gaacaggaga gagatgcaca ggagcatggc
      421 ggaccttgcg gcgaatgtct tattcatcag gcgaccccca gtaaggattt tgcttcgcgg
      481 agctcttcga tgtatatggt gcgctccggg aaggccggtg cggaaccgtt catgttgaaa
      541 ccgatgggca ggccgacctc ctcgaacatc cccctgtccc cctccccgtc acctatcgcg
      601 gaggcctccg cgggagacag cccgaggcgc tcgagagttc ggcggacgat ggggcccttc
      661 aggtcgtaat cgacatgtat cgatatctct ccggacaggg taccgtcgcg ggaaaggagg
      721 tcgttggaga aggcaaggtc aacggaaagc tctctgcgca ccttttcgac gaggagagac
      781 agacccgttg agacgatgac ggtgaccatg ccggccactt tgagggaccg gatgaggtca
      841 cgcgcgccgg gttggtatga tatttccccg atgacggctt ccacgtcacc gacggagaga
      901 cccctccaga ggagtgcgtc cctcctgcag aactcgtggt aatcgatcgc gccctgcctg
      961 aagagggcct ggtactcgtc ggcgttgtcg ctccacagcc ccaggcgacg atggaggtat
     1021 tcccagctgc tcttgacggt ggtaagggtg ccgtcgcagt cgaggaacac tatcctgatg
     1081 gccatgggtc ttatatatca cccaccgcaa aaaaaagcta tcccttagag ccccctggtg
     1141 gactataata gtaccgcgga gggatttgga aggaatgagc gaggagaagg aggtccgcga
     1201 ggcggccgac tgggacgaaa ggtatcgcaa gggtttctac gacggccccg acggccccca
     1261 cgacctgctt gtgcggtacc ggcacctgtt cggcggccgg gtggccgaca tcgccatggg
     1321 cagcgggagg gacgccctgt ttctcgcgtc tgcgggcatt gccgtgacgg gtctcgagcg
     1381 ttcatgggaa ggcctgagga tcgccggtca ggccgcggcg gcccggggac tctccatcgc
     1441 gatggtgcag ggcgacgcgg cgaggctgcc gttcaggaaa gactctttcc agggggtcat
     1501 cgtcttctac ttcctgctcc gggagatcgc cccggacatc gcggccgtgc tcgacagggg
     1561 tggtatactt atgtatgaga cattcctcaa ggcgggcggc gacgtggacc atcccaggaa
     1621 ccccgccttt cttcttgatg agggggaact tccggccctc ttccccgggt tcgacctcat
     1681 ccattacgat gaagggttga gggagcataa agggagaatg cgagcgacgg cgcaactggt
     1741 ggcgaggaaa agatgatcgt tgagtggaac ccggagcggc cgaagaagaa ggcaaccgag
     1801 ctgatcgtgc gaaccatgca ggaaggcggg atcatcgcct accccacgga tacccattac
     1861 gggatcgggt gcgacctctt caacatcagg tccatacgga aactgtacgc gatgaagcgc
     1921 ctcgacccga aaagggcgct cagcatcata tgcagggaca tgaaggacat aagcacctac
     1981 gccgtcttga gcgatttttc cttcgacatc gtcaaatggt acctgcccgg gccctttacc
     2041 tttgtcctca aggcgaggaa gatcatcccc aagcttctca tgacggacag gaaggaggtc
     2101 ggagtgagga taccggccca cgccgtaccg gtgggaatag cggtgctgtg cggccggccc
     2161 gtcatcaata cgagcgcccg catcgccggt gaagaggtca tgaccgaccc gcgggtgatc
     2221 gaaaagacct tcggggacaa catctcgctt gtcgtggatg gagggatcct ggtaagcgag
     2281 ccgtcgacgg tggtcaggct cgccgacgat gtcgcggagc tggtgaggga agggaaggga
     2341 gcccttgacg accgcatttc ccggtagggg gttcagaatg accgagaggg atgtgcagga
     2401 agggagtgcc gggatcatag ggaactacag gaaccacgat cttcacgcgc gggtcggggc
     2461 catcattcgg gagcattccg agaacaggaa ggatatcaga agcgttgtcc gtgacgcgat
     2521 cccctggccc cgggtggaga gcgttctcga tctcggatgc gggtacggct ggttcgagga
     2581 ggtcatgccc ggggggctcg atctcatcgt cgggatagac tgtcttcccg agaacgaggc
     2641 cccgttcctc gcggccgcga gggcccgggc gcgtgaggcg gtctttcgcg ccatgactct
     2701 gccggcgccg acggggctgc cggcggaccg cttcgacctt gcgctgtccc tctattccct
     2761 ctatttcttc cccggtgccg tcgctgaggc agcgcgggta ctcaaacccg gggggctctt
     2821 cctgtgcctc acccacagct cctccatgct tgaggagggc cagcactatt tcgatttcag
     2881 gaacctgaga aaggtcattg agcggttctc cgcggagaac ggcgaggcgc tcctcaagcg
     2941 gcatttcaca acggtgacct gtatcgacta ccccaacgcg ctcgtgttcc gcgagggcga
     3001 cgaggattcc ctcgccctgt acatagattt caagagagag ttcatcgaaa aggacgttga
     3061 tccccgcgtc gtcagggaca cgatgctctc cgagcttcgc cgcaagggcg agctcagctt
     3121 caacaagaac gacaggatat tcgtggcggg gaaatgaaga gaaggatcta ttgcgcccat
     3181 tgcggaacgc ctctcggcac ggacttcctc gagggcaagc acaggcaggt gtgcggcgca
     3241 tgcgacacgg tctattacga gaacccgctg cctgtcgcgt cggtgatcct tgccaacggc
     3301 aagcgcgaga tactccttgt gcgccgcgcg aaggagccct tcaaggacat gtggtgctgc
     3361 cccatcggtt tcgctgagac gggggaaagc atagaggacg ccgcccttcg cgaactgaag
     3421 gaggaggccg gggtggaggg caggatcgtg cagctcctcg acgtgggttc ccacgtgaac
     3481 ccgctgtacg gcgagcttct catcgtcacc ttcgaggtcg agaaaacagg cggtcaggag
     3541 agggccggcg acgacgcctc ggaggcgaga tatttccccg tcatgaacct tccaaagctt
     3601 gccttcgatt cgcaggtgaa ggccctggct aggttcgtgg agctgaagaa ggacctctgg
     3661 agcatccacg attccttcga aaccttcgtg gagagcaccg tgaaggacag gatcggctac
     3721 cccgtcgtcc ttttgtccga cgagctcgcc aatgccgtgc aggagaactc ggagaagatc
     3781 gtcaacctgt ggctcgacga catcacgaca aacccgtcga cacgttccta ccggcgcgtc
     3841 agcagggagg ccctgcgttc gagggccatg gtcataatgg gccagttcgg agcgtggctg
     3901 agaaaagaga agagcgaggg cgagttcacc tctttctacc gcagcctcgg cagggagcgc
     3961 aacgagggtg gggtgcccct ggaggaggcc atcagctcct tgagcctcct gaagaaacat
     4021 gtgtggatgt tcacctattc cttcggcgtc tgggagaagg ccgtcgacat ctaccgcat
//
LOCUS       DAOQTR010000145         4079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24650_length_4079_cov_3.243290, whole genome shotgun sequence.
ACCESSION   DAOQTR010000145 DAOQTR010000000
VERSION     DAOQTR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24650_length_4079_cov_3.243290"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..526)
                     /locus_tag="PKN85_04670"
     CDS             complement(98..526)
                     /locus_tag="PKN85_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HNT43729.1"
                     /translation="MDRKRIDRIIEIKEKLKKDKEREVEETTAKMAAIRAEINAVDGQ
                     IDDNYAKLSARSISGNDFAVIKDYLDYLDVQKSSLLGERASMQETLDLLQHELYEYAR
                     ELKMLGKLQDKIVRAFKKTENRREQKLLDEMALRLDDKRM"
     gene            complement(712..2037)
                     /locus_tag="PKN85_04675"
     CDS             complement(712..2037)
                     /locus_tag="PKN85_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNT43730.1"
                     /translation="MEDSIDRTMSLARDMLSNLYPYRVYGKVNQVVGLVIEGKGPISS
                     VGDSALIYPVDSTVPIDAEVVGFKEGKTLLMPLGDLRGVGIGSRILSRKKTVDVAAGE
                     GLLGRVIDGLGNPMDGKGPLKCTESVPIYRETVNPMRKKRITEPLDLGIRSINGLLTC
                     GKGQRIGIFAGSGVGKSVLLGMIARHTEANVNVIGLIGERGREVREFIERDLGDGIRN
                     SVVVVATSDQPPLIRVRGAFLTIALAEYFRDRGNDVLLLMDSLTRFSMAQREIGLAIG
                     EPPTSKGYTPSVFSLLAKVLERAGNGEGTGTMTAIYTVLVEGDDLDDPIADSARSILD
                     GHVVLSRKLADANHYPPIDVLRSVSRLMKDISPKDQTEYASRIVEILAEFERAEDLIN
                     IGAYTPGNNQKVDHAISMIDKVRTFLQQGVDERVPLEETLNSMKILFYV"
     gene            complement(2021..2656)
                     /locus_tag="PKN85_04680"
     CDS             complement(2021..2656)
                     /locus_tag="PKN85_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNT43731.1"
                     /translation="MKDQEMETFTLHDLADEMPEERTFDFIRLFDEEEARVSEDEAAP
                     EAPTTVNIEEEARRVFEEAFAQGEKAGHEMGMKKVEPMIKRLGAFMGELSLFREGLVK
                     RAENLSTELALIFAEAIVLRECAEHRDAVLAMVRKALSLCEERSGTTIRLRSEDADLI
                     PEGGLGQFKIVRDDTMREPGFIIETNFGDIDGRISVQMEELRKEYLNGRFD"
     gene            complement(2653..3654)
                     /gene="fliG"
                     /locus_tag="PKN85_04685"
     CDS             complement(2653..3654)
                     /gene="fliG"
                     /locus_tag="PKN85_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNT43732.1"
                     /translation="MNPEDLSGVRKAAILLLTMDDEVTKDVMKGLDEEEIEAIGKEIT
                     NLKLIPDHVVHRVQEEFMTKVSKRSKNVVGGESKFRLLVKKSFDEERAEILLGNLDTK
                     KGVPGEFLKRCDPRVLANVIREEHPQTMALVLSVLGTKKAGDAISCLPERVQTDVVVR
                     MANLEKVDLKTLAEVESVIRDQLESTMGGAEGKQLGGVEVVASILNQMDRTLESELLG
                     KLEETNPDLAEKIRQLMFTFEDLAHLDDRSIQVLLKEVSSEDIGIALKGASDSMKEKI
                     YSNMSERAAAMLKEDLEAMGPVRLSDVEKAQVKIAMVAKKLESEGKIFLSRGNEEFI"
     gene            complement(3658..>4079)
                     /locus_tag="PKN85_04690"
     CDS             complement(3658..>4079)
                     /locus_tag="PKN85_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43733.1"
                     /translation="GIVARAVGIDEARGDKIEVLNVPFETEGLADEKGLLDAAERKEM
                     IFSFSRYGFYALIIAALFFFVIKPVLGFLKSRADRAPMYKVKDVYMKSGGGSGTTAVT
                     GDQPVAIESKQQAAINDVLRDKVVVGSVIKEWVKEGT"
BASE COUNT          707 a         1278 c         1129 g          965 t
ORIGIN      
        1 gaggagcgaa gcgacgaacg ggcgtgagat aatgctcttg agcctctcct cccgctctca
       61 ccccatgatc agggtgacaa acgggcgtga agtgtgttta cattcttttg tcgtcgagtc
      121 ttaaggccat ttcgtcgagg agtttttgtt cgcggcggtt ctcggttttc ttgaaggccc
      181 ttactatctt gtcctggagc ttgccgagca tcttgagctc ccgggcgtat tcgtagagct
      241 cgtgctgcag gaggtcgagg gtctcctgca tggatgccct ctcgcccagg aggctcgact
      301 tctggacgtc caggtaatcg aggtagtcct ttatcacggc aaagtcattg ccggagatgg
      361 aacgggcgga gagcttcgcg tagttgtcgt cgatctggcc gtccacggcg ttgatctcgg
      421 cgcggatggc ggccatcttc gccgttgtct cctccacctc gcgctccttg tccttcttga
      481 gcttctcctt gatctcgatg atccggtcta tgcgcttgcg atccatccta accccggtcg
      541 aaaccgcttg agggaaaaat aaggggcttg aatcgcttga atagcttgag tcgcttgagg
      601 gttagagaac caagggcaga gacctcgagt cggggggtga tatccccggc gtgatcccgg
      661 agctgctctg tttttaagcc attcaagcca ttcaagctat tcaagccgcc tttagacata
      721 gaatagtatc ttcatactgt tcagcgtctc ttccaaaggt acgcgttcgt cgacgccctg
      781 ctggagaaag gtccttacct tgtctatcat ggagatggcg tggtccactt tctggttgtt
      841 cccgggcgtg taggccccga tgttgatgag gtcctccgcc cgctcgaact cggcgaggat
      901 ctctactatc cggctcgcgt actccgtctg gtccttcggg cttatgtcct tcatgagcct
      961 cgagacgctg cgcagcacat cgataggcgg gtaatggttg gcatcggcga gctttcgcga
     1021 aaggaccacg tggccgtcga ggatggaccg tgccgagtcc gcgattgggt cgtcgagatc
     1081 gtcgccttcc acgaggacgg tgtatatcgc ggtcatggtc ccggtcccct ctccgttgcc
     1141 ggccctctcg aggaccttcg ccaggagcga gaacacgctc ggagtgtaac ccttcgacgt
     1201 cgggggctcg ccgatggcga ggcctatctc gcgctgcgcc atgctgaagc gcgtcagcga
     1261 gtccatgagg aggagaacat cgttccccct gtcccggaag tactcggcga gggctatggt
     1321 gagaaaagcg cccctcaccc gtatgagggg aggctggtcc gatgtcgcga cgacgacgac
     1381 ggagttcctg atcccgtcac caaggtcgcg ctcgatgaac tccctcacct cgcggccgcg
     1441 ctctccgatg agaccgataa cattcacgtt cgcctcggta tggcgcgcga tcatccccag
     1501 aagcacgctc ttgcccacgc cggagccggc gaagattcct atcctctgtc cctttccaca
     1561 cgtcaggagc ccgttgatgc tgcgtatgcc aaggtcgaga ggttcggtga tgcgcttctt
     1621 gcgcatggga ttcaccgttt cgcgatagat gggcacggac tcggtgcact taaggggccc
     1681 cttcccgtcc atggggttcc ccaggccatc gatgacgcgg ccgaggagtc cctctccagc
     1741 ggctacatcg acggtcttct tcctggagag tatcctcgaa cctatgccca cgccccttaa
     1801 gtccccgagg ggcatcagca gggtcttgcc ttccttgaac ccgaccacct cggcatcgat
     1861 gggaaccgtg ctgtccaccg ggtagatgag ggccgaatca cccacggagg agatggggcc
     1921 ctttccctcg atgacaagac caaccacctg gttgaccttt ccgtagacac ggtaggggta
     1981 aaggttcgac agcatgtccc tcgccaggga catcgtacgg tcaatcgaat cttccattga
     2041 gatactcttt tctgagctct tccatctgga cggagatccg gccgtctatg tcgccgaagt
     2101 tcgtctcgat gataaagccc ggttcgcgca tcgtatcgtc cctgacgatc ttgaactgcc
     2161 ccagtccgcc ttccgggatg aggtccgcgt cctcgctcct caggcggatc gtcgtcccgc
     2221 tcctctcctc gcagagggaa agggccttcc tgaccatcgc gagaacggcg tcgcgatgct
     2281 ccgcgcattc cctgaggacg atggcctccg cgaagataag ggccagttcc gtcgagaggt
     2341 tctcggccct cttcacgagt ccctccctga aaagggaaag ctctcccatg aaggcaccca
     2401 gtctctttat catgggctcc accttcttca ttcccatttc gtgtccggcc ttttcacctt
     2461 gagcgaaggc ctcctcgaat accctgcgtg cctcttcctc gatattgacc gtcgtcgggg
     2521 cctcgggggc cgcctcgtct tcggagaccc gggcctcctc ttcatcgaaa agcctgatga
     2581 aatcgaaggt tctttcctcc ggcatctcat cggcgaggtc atgcagggta aaggtttcca
     2641 tttcctggtc tttcaaatga actcttcatt cccccgggac agaaagatct tgccctcgct
     2701 ttcgagcttc ttggcgacca tggcgatctt cacctgggcc ttttcgacgt ctgagagacg
     2761 aacggggccc atggcctcga ggtcttcctt gagcatggcc gcggcgcgtt cggacatgtt
     2821 ggagtatatc ttttccttca tgctgtccga ggcacccttg agggcgatac cgatatcctc
     2881 ggacgagacc tccttgagaa gcacctggat gctgcggtcg tcgaggtggg cgaggtcttc
     2941 gaaggtgaac atgagctgcc ggatcttttc cgccaggtcg ggattggtct cctcgagctt
     3001 tccgagaagc tccgattcca gggtcctgtc catctggttc agtatgctgg ccaccacctc
     3061 cacgccgccg agctgcttgc cttcggcccc gcccatggtg ctctcgagct ggtcccgtat
     3121 aacgctctca acctcggcca acgtcttcag gtcgaccttt tcgagattgg ccatgcgcac
     3181 gacaacgtcc gtctgcaccc tctcaggcag gcagctgatg gcgtctcccg ccttcttcgt
     3241 cccgagcacc gacagaacca gggccattgt ctggggatgc tcctcccgta tcacgttcgc
     3301 gaggaccctc ggatcacagc gcttcaggaa ctctccggga acacccttct tcgtgtccag
     3361 attgccaaga agtatctccg cccgctcctc gtcgaagctt ttcttgacga ggaggcggaa
     3421 cttgctttcg ccgccaacga cattcttgct cctcttcgag accttcgtca tgaactcttc
     3481 ctggaccctg tggaccacgt ggtcggggat gagcttgaga ttcgtgatct ctttgccgat
     3541 cgcctcgatc tcttcctcat cgaggccctt catcacgtcc ttcgtcacct cgtcatccat
     3601 ggtgagaagg agaatggcgg cctttctgac tcctgacaga tcttcggggt tcatcgatta
     3661 cgtcccttcc ttcacccatt ccttgatcac ggatccgaca acgaccttgt ccctcaggac
     3721 atcgttgatc gccgcctgct gcttgctctc tatggcgacg ggctggtctc ccgtcaccgc
     3781 ggtggtgccg ctaccgccgc cgctcttcat gtacacgtcc ttgaccttgt acatgggagc
     3841 gcggtccgcc cgcgatttaa ggaaaccgag cacgggcttg atcacgaaga agaagagcgc
     3901 cgcgatgata agggcgtaga agccatacct gctgaagctg aatatcatct ccttccgctc
     3961 cgccgcgtcg agaagccctt tttcgtcggc gaggccttcg gtctcaaagg ggacgttgag
     4021 gacctcgatc ttgtcgcccc gcgcctcgtc gataccgacg gccctcgcca cgatccccc
//
LOCUS       DAOQTR010000146         4071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24732_length_4071_cov_3.431773, whole genome shotgun sequence.
ACCESSION   DAOQTR010000146 DAOQTR010000000
VERSION     DAOQTR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24732_length_4071_cov_3.431773"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKN85_04695"
     CDS             complement(<1..437)
                     /locus_tag="PKN85_04695"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43734.1"
                     /translation="MKKVLEALPADFPVSERPYDEMAKEMGMSGAALIDELVALKKAG
                     IIRRIAAMVAHRSVSYEGNAMVVWRVPEAEVEKVGTVMAGFDEVSHCYERDTGGYWDY
                     NLYTMVHGRTREECLATIARMASRSGIGDYRVLFSLREFKKTS"
     gene            608..1771
                     /locus_tag="PKN85_04700"
     CDS             608..1771
                     /locus_tag="PKN85_04700"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNT43735.1"
                     /translation="MVKPASRVEQIPPYLFARIDKKKAEVRQKGMDLVDFGIGDPDIP
                     TPGNIIARMLEATQDPQNHRYPSYEGMLEFRQAAASWYKKRFDVDLDAATEVVTLIGS
                     KEGIAHIPWAYAEEGDIVLVPSPGYPVYKIATMFAGATPYIMPLKEENGFLPRYEDIP
                     EEILKKAKLLFINYPNNPTGACADDAFFEKTLELAKRYDILVCHDAAYSEIAYDGYRP
                     KSILEFDREKKHCLEFHSLSKTYCMTGWRIGFAVGCADGIYNLGKLKTNIDSGAFQAI
                     QYAGIEALTGSQASVAGLNTRLEKRRDMVVEAFKTLGIDVKRPKATYYIWARVPKGYT
                     SSDFCEKLIEETGIVVTPGSGFGDEGEGYFRISITISEDKIAEAAKRLRSFKI"
     gene            complement(1939..2856)
                     /locus_tag="PKN85_04705"
     CDS             complement(1939..2856)
                     /locus_tag="PKN85_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT43736.1"
                     /translation="MRDRTGSLLTDRSYRRLLDSLYEGVFVVDRDRTILYWNDAAEQM
                     TGYKRGEVVGKHCWDGFLTHVDNRGIRLCADRCLLQEAMELKKSVEGKVFFHHRNGHE
                     VPVTVRTTPILGDRGKVLGAAQIFSDNSSTEDLGRKIRELERMALLDPLTKLGNRRYG
                     QMNLSGKLREFKRYGWPFGIFFIDIDKFKAVNDTYGHEAGDRVLRLVAAIIRNGLRSS
                     DVVVRWGGEEFVAIVASVDEEKLRHVGEKLRALVERSSISLEKETKNVTISIGATLAR
                     KKDSAAALIKRADALMYQSKKEGRNRLSI"
     gene            complement(2853..>4071)
                     /locus_tag="PKN85_04710"
     CDS             complement(2853..>4071)
                     /locus_tag="PKN85_04710"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNT43737.1"
                     /translation="WKSYYASTNSFDEGHLVDLAGQLSRYGSDRGEGAVAIAGGEGAA
                     AYPFSIGEDPGGVPVEKKLALVRDFESMARRMESRITQVRVMYRDTRQNVRIATTSEG
                     ARNDSRMQVVLTVLLVGRDGSEMQTSYEAVGGFHGFEFFTDELMEELARKTVRRLQGL
                     LAAREAPMGMKTVVLASEAGGTMIHEAIGHGLEADLAMEGLSCYKGLLGAGIASPLVS
                     VVDDATLPHMRGTYAFDDEGVPSERTVLVEKGVLRNYLFDRFHAMKHSMASTGNGRRE
                     SYRFRPIPRMSNTMILPGTDDPEKILASVDDGILVVKMGGGQVDTVRGDFVFEISEGY
                     IIERGTVGPMIKNATMMGNGLKVLRDIDMVGTDLGFGIGTCGKDGQGVPVADAQPTLR
                     IPGIIVGGRGGQR"
BASE COUNT          837 a         1279 c         1118 g          837 t
ORIGIN      
        1 aaggatgtct tcttgaactc tctcaggctg aaaagcaccc ggtaatcccc gatgcccgac
       61 cgtgacgcca tcctggcgat cgtggccagg cactcttccc ttgtcctgcc atgcaccatg
      121 gtgtaaagat tgtagtccca gtaacccccg gtgtcgcgct catagcagtg gctcacctca
      181 tcgaaacccg ccatgaccgt gccgaccttc tccacctcag cctccgggac gcgccacacg
      241 accatggcgt tgccttcata ggaaactgaa cggtgcgcga ccatggcggc tatcctccgg
      301 atgatgccgg ccttcttcag cgccacgagc tcatctatga gggccgctcc cgacatcccc
      361 atttctttcg ccatctcgtc gtagggcctt tcggatacgg ggaaatcggc gggcagcgct
      421 tccaggacct ttttcatgtg gttctattct tacacatcgg gaaaaacgtt ttcaaacaaa
      481 ttcggagcca aaaaagactt tacattgcac ccgctaacat gttgtaacta acgaatccgg
      541 ccctgccggc gcgagcccgc accaaggcgc cgaacgcgtt gaaacaatcc tttaaggagc
      601 ttgtaagatg gtaaaacccg catccagagt agaacagatc ccgccgtatc ttttcgccag
      661 gatagacaag aagaaggcgg aggtcaggca gaaagggatg gatcttgtcg atttcgggat
      721 aggcgatccc gatatcccta ccccgggcaa tatcatcgcc agaatgctgg aggcaacaca
      781 ggacccgcag aaccaccgct atccgagcta cgagggaatg cttgagttcc gccaggcggc
      841 ggcctcatgg tacaagaaac gttttgacgt ggacctagac gccgcgacgg aagtggtgac
      901 cctcatcggc tccaaggagg gcatcgccca catcccctgg gcctacgccg aggaagggga
      961 catcgtcctc gtcccttccc ccgggtatcc cgtctacaag atagcgacca tgttcgccgg
     1021 ggcgacgccc tacatcatgc ccctcaagga agagaacggg ttccttcccc gctacgagga
     1081 catccccgag gagatcctga agaaagcaaa gcttctcttc atcaactatc cgaacaaccc
     1141 caccggagcc tgcgccgatg acgccttctt cgaaaagacg ctggagctcg cgaaacgcta
     1201 tgacatactc gtctgccacg acgccgcgta cagcgagatc gcctacgacg gatacaggcc
     1261 gaagagcatc ctcgagttcg acagggaaaa gaagcactgc ctcgagttcc actccctttc
     1321 caagacctac tgcatgaccg gctggaggat cggcttcgcc gtgggttgcg ccgacggcat
     1381 ctacaacctc ggcaagctga agacgaacat cgactccggc gccttccagg ccatccagta
     1441 cgccggcatc gaagccctca caggcagcca ggcctccgtt gccgggctca acaccaggct
     1501 cgagaagagg cgcgacatgg tcgtggaagc tttcaagacc ctggggatcg acgtcaagag
     1561 gcccaaggcc acctattaca tctgggcacg ggtcccgaag gggtacacct cctccgattt
     1621 ctgcgaaaag ctcatcgagg aaacgggcat cgtcgtcact cccggcagcg gcttcggcga
     1681 cgaaggcgag ggctacttcc gcatctccat cacgatcagc gaagacaaga tagccgaagc
     1741 agcgaaaagg ctcaggtcgt ttaagattta aaggcggctt gagtcgcttg aatcgtttga
     1801 gtcgcttgag ggtgaaagac tgaaaaggtt ttcagagtat tgtgttgcgc catgagggat
     1861 agtcttttgt ggttgggttc ttttaacctt caagcgactc aaacgattca agccctttat
     1921 tcttattcaa gccggtttct agatggatag cctgtttcgg ccttcttttt tgctctggta
     1981 catgagggcg tcggccctct ttatgagggc ggcggcggag tcttttttcc gggccagggt
     2041 ggcgccgatg gatatggtca cgttcttcgt ctccttctca agggagatgg acgatctctc
     2101 gacgagggcc cggagtttct ctcccacgtg acgcagtttc tcttcgtcca cgctggcgac
     2161 tatggcgacg aattcctcgc cgccccacct aacgaccacg tccgaagacc tgaggccatt
     2221 gcggatgatg gctgccacaa ggcgcaggac acggtccccg gcctcatgac cgtacgtgtc
     2281 attgacagcc ttgaacttgt cgatatcgat gaagaagatc ccgaaaggcc agccgtagcg
     2341 cttgaattcc ctcagcttcc ccgaaaggtt catctgaccg taccgcctgt tcccgagttt
     2401 cgtcagcgga tcgagaagcg ccatccgttc cagctcgcgg atcttgcgcc ccagatcctc
     2461 ggtggatgaa ttatcgctga atatctgagc ggcgccgagc actttgccgc ggtcgcccag
     2521 tatgggcgtc gtccgcacgg tgacggggac ctcgtggccg ttgcgatgat ggaagaagac
     2581 cttcccctcc acgctcttct tcaactccat cgcctcctgg aggagacacc gatccgcgca
     2641 gagcctgatg ccgcggttat ccacgtgtgt gagaaaaccg tcccagcagt gttttccgac
     2701 gacctcaccg cgtttatatc ccgtcatttg ctcggcggca tcgttccagt agaggatggt
     2761 acggtccctg tcgacgacaa aaacaccctc gtaaaggctg tcgagaagcc tccgataaga
     2821 cctgtcggtc aacagactgc ccgtcctatc tctcatctct gcccgccgcg acccccgacg
     2881 atgatccccg gtattctcag ggtgggctgc gcgtcggcca cgggaacacc ctggccatcc
     2941 ttgccgcagg tgccgatccc gaacccgagg tccgtgccca ccatgtcgat gtccctgagg
     3001 accttgagtc cgttccccat catcgtcgcg ttctttatca taggccccac cgtgcctctc
     3061 tcaattatat acccttcgga tatctcgaag acaaagtctc cgcgcacggt atccacctgt
     3121 ccgcccccca tcttgacgac aaggatgccg tcgtccacgg aggccaggat cttctcgggg
     3181 tcatcggttc ccggaaggat catcgtattg ctcatcctcg ggatgggcct gaaacggtag
     3241 gactcacgcc tgccgttgcc cgttgaggcc atggagtgct tcatggcatg aaagcggtcg
     3301 aagagatagt tcctgagaac acctttttca acgaggaccg tcctttcgga ggggacgcct
     3361 tcgtcgtcga acgcgtaggt cccccgcatg tggggcagtg tcgcgtcgtc gacaacgctc
     3421 accaggggcg atgctatccc ggcgccgaga agccccttgt agcaggaaag cccctccatg
     3481 gcaaggtcgg cctccagacc gtgtccgatg gcctcgtgga tcatcgtgcc tcccgcctct
     3541 gaggcgagga cgacggtctt catccccatg ggcgcctcgc gcgccgcgag aaggccctga
     3601 aggcgtctta cggtcttccg cgcaagttcc tccatgagtt catcggtgaa gaattcgaaa
     3661 ccatggaagc cgcccaccgc ctcgtaggat gtctgcatct ccgagccgtc cctgccgacg
     3721 agaagcaccg tgaggacgac ctgcatgcgg ctgtcgttcc tcgcaccctc agaggtggtg
     3781 gctatcctga cgttctggcg ggtgtcgcgg tacatgacgc ggacctgggt tatgcgggac
     3841 tccatcctgc gggccatgga ctcgaagtcc ctgacgaggg caagcttctt ttccaccgga
     3901 acgcccccgg ggtcttcccc tatggaaaag ggatacgccg cggcgccttc gcccccggcg
     3961 atggcgaccg cgccctcccc acgatctgac ccgtagcggg agagctgccc ggccagatcg
     4021 acgagatggc cttcatcgaa ggagttcgtc gaggcgtaat aacttttcca g
//
LOCUS       DAOQTR010000147         4047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_24948_length_4047_cov_3.255511, whole genome shotgun sequence.
ACCESSION   DAOQTR010000147 DAOQTR010000000
VERSION     DAOQTR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24948_length_4047_cov_3.255511"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..397)
                     /locus_tag="PKN85_04715"
     CDS             complement(80..397)
                     /locus_tag="PKN85_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNT43738.1"
                     /translation="MVTDRAPRDDGKKAEDRMIENVGHKAARKLRARERQDRGIWFGL
                     GMFGLVGWSVAVPTLVGLAVGVWIDMRWPGRISWTLTGLIAGVALGCLNAWYWVKRES
                     GHD"
     gene            complement(387..779)
                     /locus_tag="PKN85_04720"
     CDS             complement(387..779)
                     /locus_tag="PKN85_04720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNT43739.1"
                     /translation="MNLRILLPDHVFLEERVRKVIAEALNGHFCLLPRHVDLVTALVP
                     GLFSYESEDGRERFLAVGEGVLVKSGREVIVSVRNAMRGPDLGQLRRIVEEEFKILDE
                     HERAARSAVSRIEVNFIRRFLEIREHGD"
     gene            complement(776..2161)
                     /gene="atpD"
                     /locus_tag="PKN85_04725"
     CDS             complement(776..2161)
                     /gene="atpD"
                     /locus_tag="PKN85_04725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNT43740.1"
                     /translation="MRERGSTGTGMVVSVRSSVVDVRFPEHLPPLYNILRAGDRGAVA
                     IEVVNHIDSRTTRGIALTSTEGLALRSEVVDTGHSLRVPVGKMVLGRVLNVFGETIDR
                     GEALQDGEKRPIHREPVSLRRRSTGSEIFTTGIKAIDVLSPLERGGKAGLFGGAGVGK
                     TVLIMEMIHNMVGQHEGVSLFCGIGERCREGEELYREMKAAGVLDNTVMVFGQMNEPP
                     GARFRVGHAALTIAEFFRDDMRQDVLLLIDNIFRFIQAGQEVSGLMGRLPSRLGYQPT
                     LGTELAEFEERISNTTSGAITSVQAVYVPADDFTDPAAVHTFGHLSASIVLSRKKASE
                     GLYPAIDPLQSSSSMLVPHLVGGRHYSIAQEIRRTLATYEELKDIISMLGMEELSRED
                     RRSVHRARRLERFLTQPFFVTEAFTGYDGKTVAVEDALDGCEKILNDEFSDVPERKLY
                     MLGGIGEVKKR"
     gene            complement(2261..2644)
                     /locus_tag="PKN85_04730"
     CDS             complement(2261..2644)
                     /locus_tag="PKN85_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43741.1"
                     /translation="MFIKHLLISALISLILTGIFVLVFRRRGPWNSFLIFFVVVFLGA
                     WAGGIWYPARGPAIGGVTWLPFFIVGLVLSLLLVAVIPPPEETTVELLKEGEKDTEAR
                     KKLVLGIYFWLMVLALAVMIVARYV"
     gene            complement(2683..3126)
                     /locus_tag="PKN85_04735"
     CDS             complement(2683..3126)
                     /locus_tag="PKN85_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43742.1"
                     /translation="MRRFLALFVCMGVMTLAGCGGKEEPAETKKPVTMEEVKKEAGEA
                     AQTAKAYTRQQMDEYRQQVQAKLEDYDKKLDEMRAKAEKMKKEARAEMEQQIAELRKK
                     REEAAKELEKMQSASGKAWEDLKTGLDSALEEMDKAYQQAASRFK"
     gene            complement(3174..3584)
                     /locus_tag="PKN85_04740"
     CDS             complement(3174..3584)
                     /locus_tag="PKN85_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43743.1"
                     /translation="MYCDVIAFERRDRRRVLFENSSFLAVVPYAAEVPFEVWILPIEH
                     QASFGQISDRAKQDLAAAMEDILGRLHRHFSDLNYNYVIVTSPQYRAREPQMHWYLQI
                     RPRLTTRAGFEIGSGISINPSLPEEDAEVLNSLM"
     gene            complement(3574..>4047)
                     /locus_tag="PKN85_04745"
     CDS             complement(3574..>4047)
                     /locus_tag="PKN85_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43744.1"
                     /translation="PSCPFCAGNEGMLPPLVLEMPGKGGDGWATRVVPNKFPALTPKG
                     DTVRACDGIYVTMPGHGRHEVIIESPYHNEQIGTMSVDQIRIVIETYHRRFVDLMKEH
                     GTMMVLIFRNHGARGNVPHPPPFTGRRDGHGSQLHPLEGRRRPAIFRRLGAMRVL"
BASE COUNT          801 a         1315 c         1044 g          887 t
ORIGIN      
        1 atggaggaat cccaggaccg ctccagatac caggaagatt aagtagacgg caatatccat
       61 ggtgatctgc tccttatcct cagtcatgtc cgctctcgcg tttcacccag taccaggcat
      121 tgagacagcc cagcgccact cctgcgatga gtcccgtaag cgtccacgag atgcgaccgg
      181 gccaccgcat atcgatccac acgcccacgg ccagaccgac cagtgtcggc acggctacgg
      241 accatccgac aagaccgaac atccccagac cgaaccagat gcccctgtcc tgtctctccc
      301 tggccctcaa tttccgggcg gccttgtgtc ccacgttttc gatcatcctg tcttccgcct
      361 tttttccgtc gtctcgcggc gcgcggtcag tcaccatgtt ccctgatctc cagaaatctt
      421 cgtatgaaat tgacctctat cctggacaca gccgatcttg ccgctctttc gtgttcgtca
      481 aggatcttga actcctcctc aacgatccgt ctcaactgtc ccagatcggg cccccgcatg
      541 gcgttcctga ccgatacaat gacctcgcgg ccggacttca cgagaacgcc ttcgcccacg
      601 gcgagaaagc gttcccttcc atcttccgat tcgtacgaga agagccccgg aacaagagcg
      661 gtgacgagat cgacatgacg gggcagcaga cagaaatgac cgttcagggc ctccgcgatc
      721 accttcctca ccctttcctc caggaacaca tggtccggta gcaatattct caggttcatc
      781 tcttcttcac ctcgccgatc ccgccgagca tgtacagctt ccgctcaggc acatcggaaa
      841 actcgtcgtt gagtatcttc tcgcagccgt caagggcgtc ttcgacggcg acggtcttgc
      901 cgtcgtatcc cgtgaaggcc tccgtcacga aaaaaggctg cgtcagaaac ctttcaagac
      961 gtctcgcgcg gtgcacgctc cgtctgtcct cccgggagag ctcctccatg cccagcatgg
     1021 aaatgatatc cttcaactcc tcataggtgg caagcgtcct gcgtatctcc tgcgcgatgc
     1081 tgtagtgtct gccgccgacg agatgaggta cgagcatgct ggaggaggac tgcagcggat
     1141 cgatggccgg atagagccct tcacttgcct ttttccggga aagaacaatg gaggccgaca
     1201 ggtgtccgaa ggtgtgcacc gccgcggggt ccgtgaaatc atcggcgggc acgtagaccg
     1261 cctggaccga ggtgatcgcc ccgctggtgg tattggagat ccgctcctca aactcggcaa
     1321 gctccgttcc gagtgtcggt tgataaccca agcgtgaggg gagccggccc atgagacccg
     1381 aaacctcctg ccccgcctga atgaaacgga atatgttatc gatgaggaga aggacatcct
     1441 gtctcatatc gtccctgaag aattcggcta tggtaagggc ggcatgaccg acgcggaacc
     1501 tcgcccccgg tggctcattc atctgcccga ataccatgac ggtattgtcc agcacccctg
     1561 cagctttcat ctcccggtac agctcttctc cctcccggca gcgctctccg atcccgcaga
     1621 aaagactcac cccttcatgc tggcccacca tgttatggat catctccatg atcagcacgg
     1681 tcttccccac gcccgcgcct ccaaagagac ccgcctttcc ccctcgctcc agaggggaaa
     1741 ggacatcgat ggctttgata ccggtggtaa agatctccga gcctgtcgaa cggcgcctga
     1801 gagacacggg ctccctgtgg atcggccgct tctctccgtc ctgcagggcc tcccccctgt
     1861 cgatggtctc cccgaaaaca ttgagcaccc tgcccagcac cattttccca acgggcaccc
     1921 tcagggagtg ccccgtatcc acgacctccg acctcagggc aaggccttcg gtggacgtga
     1981 gggcaatgcc gcgcgtcgtt cgggaatcga tgtggttcac gacctcgata gcgacggctc
     2041 ccctgtcccc cgccctgagg atgttgtaaa ggggtggaag atgttcaggg aatctgacgt
     2101 ccaccacgct gctgcggacg gagacgacca tgcccgttcc cgtcgatccg cgttctcgca
     2161 taccgtccat ctccatcgtt catcttcccc gtcttctctt ccctctccat gcggcggacc
     2221 gttccgggag gagactgtgg ggatctcgct tctcccgtcc tcagacgtag cgcgcaacga
     2281 tcatgaccgc cagagccaga accatcagcc agaagtagat ccccaggacc agcttcttcc
     2341 ttgcctccgt gtccttctct ccctccttca gcaactccac cgtggtctcc tccgggggag
     2401 gtatgacggc gacaaggagc aaggacagga caagtccgac gatgaagaag ggaagccagg
     2461 tgactccacc gatcgcgggt cccctggccg gataccatat cccgccggcc caggctccaa
     2521 ggaagacgac cacgaaaaag atcaaaaaac tgttccaggg accccttctt cggaacacga
     2581 gaacaaaaat ccccgtcaag atcagggata tcagggcgga gatcaggaga tgcttgataa
     2641 acatggccac ctcgtgtcag tcgcgccggg tcggcgccaa tatcatttga aacgggaggc
     2701 cgcctgctga tatgccttgt ccatctcctc cagggcggaa tccaatcccg tttttagatc
     2761 ttcccaggct ttgccggagg cggattgcat cttctcgagc tccttggcgg cttcctccct
     2821 tttcttgcgg agctcggcta tctgctgctc catttcggcc ctggcctctt ttttcatctt
     2881 ctccgctttc gcccgcatct catcgagctt tttgtcgtaa tcctcgagtt tggcctgcac
     2941 ctgttggcgg tattcgtcca tctgctggcg ggtgtacgcc ttcgccgtct gagcggcctc
     3001 tcccgcctct ttttttacct cttccatggt gacgggcttc tttgtctcgg cgggctcttc
     3061 ctttccgccg catccggcaa gggtcatcac acccatgcaa acaaacaacg ccaaaaatct
     3121 tctcatctct ttttcctcct ttctctccta tccgtgtctc tcccctcggt ctcctacatc
     3181 agggagttca agacctcggc atcctcttcg gggagcgacg gattgatact gataccggag
     3241 cctatctcga aaccggcccg ggtcgtaagc cgcggacgta tctgcagata ccagtgcatt
     3301 tgcggctccc tcgccctgta ttgcggagaa gtgacgatga cgtaattgta gttgaggtcg
     3361 ctgaagtgcc ggtggagtct gccgagtatg tcttccatgg cggcggcaag gtcctgcttc
     3421 gcccggtcag atatctgtcc aaaacttgcc tgatgctcga tcggcaggat ccagacctca
     3481 aaaggaacct cggcggcata gggaacaaca gcaaggaatg atgagttctc gaagagaacc
     3541 cgcctgcggt cgcgcctctc aaaggcaatg acatcacagt acacgcatcg cccccaggcg
     3601 tcgaaatatc gctggccttc tccttccctc cagcggatgt aattgggaac catgcccgtc
     3661 acgacgacct gtgaatgggg gtggatgagg gacgttcccg cgcgcgccat gattgcggaa
     3721 gataaggacc atcatggtcc cgtgctcttt cataagatcg acgaagcgcc gatggtatgt
     3781 ttctataacg atcctgatct gatcgacgga cattgtccct atttgctcat tatgataggg
     3841 actttcgatg atgacctcgt gacggccatg tcccggcatt gtcacataga tcccgtcgca
     3901 ggccctgacc gtgtcaccct tcggggtcag cgcgggaaac ttgttgggca caaccctcgt
     3961 tgcccagcca tccccgcctt ttcccggcat ctcaagcacc agcggaggaa gcatcccttc
     4021 gttcccggcg cagaacggac aggacgg
//
LOCUS       DAOQTR010000148         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25004_length_4040_cov_2.861731, whole genome shotgun sequence.
ACCESSION   DAOQTR010000148 DAOQTR010000000
VERSION     DAOQTR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25004_length_4040_cov_2.861731"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKN85_04750"
     CDS             complement(<1..425)
                     /locus_tag="PKN85_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNT43745.1"
                     /translation="MMAADALTASLEDYLEAIFHIIAEKHAVKPRDIAKRLKVSYASV
                     TGALRSLSEKKLINYSPYDLITLTDEGEVAAKDVVRRHEILHDFFVEVLAVPEADADA
                     AACQMEHSIPRDIVERFVRFVEFVETCPRGGQKWIKGFG"
     gene            complement(773..1723)
                     /locus_tag="PKN85_04755"
     CDS             complement(773..1723)
                     /locus_tag="PKN85_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT43746.1"
                     /translation="MTMRLVGLHNSDQKISAVILGTWAIGGSHWGPYDEANAMRAIET
                     ALDNGINAIDTAPVYGDGHAEELIGRAIRGKRDRIFLATKCGLDIYDRSYRRDLSPAY
                     IEKDLHGSLKRLGTDCVDLYQCHWPDPQVPVEETMAALTRFRDEGKIRHIGVSNFNGP
                     QLLEALKFVALFSLQPHYSLLERSAGEDLLPICIGKNINVFPYGSLGAGMLTGKYREC
                     PRFKKGDARSFFYRYFKPAYWPRVRRLLDEVEAIAARGGTTSGAVALSWLLRQPGVTS
                     VIVGARSAEQVRGNIADVPVDLSDEDMRALDTLSSLIYES"
     gene            complement(1720..2685)
                     /locus_tag="PKN85_04760"
     CDS             complement(1720..2685)
                     /locus_tag="PKN85_04760"
                     /inference="COORDINATES: protein motif:HMM:NF022892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HNT43747.1"
                     /translation="MNRKRAISPRRLVVIIGIAALLAVFLSGTQGQAAPSKSPKQLTF
                     ATAEEAARVLADAARAKDTKAMLAIFGPEGKGIIISGDEAVNKDLYERFVKAFQEKMR
                     LDMSTDNKAFLYVGNNEWPFPVPIVRKSGRWSFDMKDGKTEILHRRIGRNELSAVQAC
                     LAYVDAQKDYAGMIGRKGGVAEYARQFLSDPGKRNGLYWESREGEEPSPLGVFMARAR
                     WQHSLLEPPAGKPVPYHGYFYKILTAQGKHAPGGAYNYIVDDRMIGGFALAAWPAQYG
                     VTGVMTFIVNQDGVVYEKDLGRVSGKAVYHMKAFNPDDSWKRVNE"
     gene            complement(2750..3970)
                     /locus_tag="PKN85_04765"
     CDS             complement(2750..3970)
                     /locus_tag="PKN85_04765"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HNT43748.1"
                     /translation="MNRAFRRTLIWVLVASLVIPVGVFAQGTGTSKPAFKQEELEQLL
                     APIALYPDDLVAQILMASTYPLEVVEAARWVKANPNLKGNQLTSALEKQNWDPSVKSL
                     VNFPSVLTMMNEKLDWTQKLGDAFLAQQKDVMNTVQKLRAKAQASGNLKTTKEQKVVV
                     QDQVIVIQSADPQVIYVPTYNPTVVYGAWPYPAYPPYYYYPPGYVAGAAMFSFAAGVA
                     VGAAWGYAWGGCNWHNGDVNVNVNKNINVNNQINRNYYANKISTNPGGHGEWKHDPSH
                     RKGVAYRDQATGAKYGQAPRPGADTRKDFRGYSPDARGSQPQAAGRSGRDSVGQGKPS
                     YASSRTQQTPRFDQGSMGKQGTSHAFEGMERGGTQTKMESERGRASMSGFGSGGERGS
                     GGGFGRSGGGGRRR"
BASE COUNT          764 a         1306 c         1085 g          885 t
ORIGIN      
        1 tacccgaaac ccttgatcca tttctgaccg ccccgagggc aggtctcgac aaactccacg
       61 aatctcacga acctctccac tatgtccctg gggatggagt gttccatctg gcatgccgca
      121 gcgtccgcgt ccgcctccgg gacggcgagt acctcgacga agaaatcgtg cagtatctcg
      181 tgcctgcgga caacatcctt cgccgcgacc tccccttcat ccgtcagcgt tataaggtca
      241 taaggcgaat aattgatcag tttcttttca gacagggaac gaagggcacc cgttaccgac
      301 gcatagctca ccttaagacg tttcgcgatg tcccgcggtt tcacggcatg cttctcagcg
      361 atgatatgaa aaatggcctc caggtagtcc tccaggcttg ccgtcaatgc gtcagccgcc
      421 atcataaaca cccccactaa taagtttagg ttgccctaat tctttacact attcctaatg
      481 gcattgcaag aaaaatgttt cggcgcgtcc ctgtccgttt tccccgcttc aaggtcccag
      541 gcgcccccgt ccggcctttg tcgaaggcca gagggtcctc gttgaactca tggaagcaat
      601 acgggcggcc agagcggaag aacctgcccg tgccatccct gccggaaaca atgcgccccg
      661 gggacagcgg agcgcatccg aattaatggg gtcaagggct cgcggacctg caccttcctg
      721 atgatggtcc gaagcctggg gtcaaagcat cgacggtccc ctcccggtct cctcaggact
      781 cgtagattag gctggacaac gtgtcgagcg cgcgcatgtc ctcatcgctc aagtccaccg
      841 gcacgtcggc tatgtttccc ctgacttgtt cggccgaacg tgcaccgacg atcacagatg
      901 tgactccggg ctgtctgaga agccacgaga gcgccacagc accggaggtc gttcccccgc
      961 gggcggctat cgcttccacc tcgtccagga gccgccgcac cctcggccag tacgcgggct
     1021 tgaagtaacg gtagaaaaag gagcgggcgt cacccttctt gaaccgggga cattcacggt
     1081 attttcccgt caacatgccg gcacccaggg agccataggg aaagacatta atgttcttcc
     1141 ctatgcagat ggggagaaga tcttccccgg cgctcctctc cagcaaagaa tagtggggct
     1201 gcaaggagaa aagggcaacg aatttcaagg cctccaggag ttgggggccg ttgaaatttg
     1261 atacaccgat gtgcctgatc ttcccctcat ctctgaacct cgtgagcgcc gccatcgttt
     1321 cctccacggg gacctgtggg tccggccagt ggcactggta caggtcgacg cagtcggtgc
     1381 cgagacgctt cagggagccg tgaaggtcct tctcgatgta agcaggggaa aggtcacgcc
     1441 tgtacgaacg gtcatagatg tcgaggccgc actttgtggc aagaaagatc ctgtccctct
     1501 tgccgcggat agcccttccg ataagctcct cggcatggcc gtctccgtaa acgggcgccg
     1561 tgtcgatggc gtttattcca ttgtcgaggg ccgtctcgat ggccctcatc gcgttcgcct
     1621 catcgtaggg cccccagtgg ctgccgccga tcgcccaggt ccccaggatg acggccgata
     1681 tcttctggtc cgaattgtgc agtcccacca gcctcattgt cattcattca cccttttcca
     1741 agaatcgtcg ggattgaagg ccttcatgtg ataaaccgcc ttacccgaga ccctgcccag
     1801 atccttctcg taaacgacgc catcctgatt cacgatgaac gtcatgaccc ccgttacgcc
     1861 atactgggcc ggccaggcgg caagagcaaa acctccgatc atcctgtcgt caacgatgta
     1921 attgtacgcc ccgccgggcg cgtgcttgcc ctgcgctgtg agtatcttat aaaagtagcc
     1981 gtggtacggc accggcttcc ctgccggcgg ctccagcaga gaatgctgcc accttgctct
     2041 cgccatgaag acgcctagag gacttggctc ctcgccttcg cggctctccc aatacagacc
     2101 gttacgtttc cccggatcgc tcaggaactg ccgggcgtat tcggccacac cgcccttccg
     2161 gccgatcatt cccgcgtagt ccttctgggc atccacatag gcaaggcagg cctgaacggc
     2221 gctgagctcg ttcctgccta tcctccggtg gagtatctcg gtctttccat ccttcatatc
     2281 gaaggaccac ctgcctgact tcctcacgat gggaaccggg aagggccact cgttgttgcc
     2341 gacatagagg aacgccttgt tgtccgtaga catgtcgagc ctcatcttct cctgaaaggc
     2401 cttcacgaag cgctcgtaaa ggtccttgtt gacggcctcg tcgccggaga tgatgatacc
     2461 ttttccctcg ggtccgaaga tcgcgagcat cgccttcgtg tccttggcac gcgccgcatc
     2521 ggcaagcaca cgcgctgctt cttcggcggt ggcaaaggtc agctgcttgg gcgacttcga
     2581 aggcgcggcc tgcccctgcg tccccgacag gaacaccgca aggagtgccg cgatgccaat
     2641 gatcacgaca agtcttcgcg gagatatggc tctttttcta ttcataacgc ggatcatacg
     2701 atttcctcct cgtaacgttc attgcacaca tcatacctgc agccgggggc taacgccggc
     2761 gaccgcctcc tccacttcgg ccgaagccgc cgcccgaccc tctctcgcct ccggaaccga
     2821 aaccggacat gctcgcgcgg ccccgctcgc tttccatctt tgtctgtgtg ccgccgcgtt
     2881 ccataccctc gaaggcgtgg ctggtccctt gtttccccat ggagccctgg tcaaacctgg
     2941 gcgtctgctg ggtccgggaa ctcgcgtacg atggctttcc ctgtcccacg gagtcccttc
     3001 cggaccggcc tgcggcctgc ggctgtgagc cccgcgcatc gggtgaataa cccctgaagt
     3061 ccttcctcgt gtccgctccc ggcctcggag cctgtccgta cttcgcccct gtcgcctggt
     3121 cccggtaggc aacacccttt ctgtgggagg gatcgtgttt ccattccccg tgcccgcccg
     3181 ggttggtcga tatcttgttc gcgtagtagt tcctgttgat ctggttgttc acgttgatgt
     3241 tcttgttgac gttgacattc acgtcgccgt tgtgccagtt gcaaccgccc caggcgtaac
     3301 cccaggccgc gcccaccgcc acgcctgccg cgaaggagaa cattgcagcc cccgccacgt
     3361 atcccggcgg gtagtaatag tatggcggat aggccggata gggccaggcg ccatacacaa
     3421 cggtggggtt atacgtggga acgtagatca cctgcgggtc ggccgactgt atcacgatga
     3481 cctggtcctg cacgacgacc ttctgctcct tcgtcgtctt gaggttcccc gaggcctgcg
     3541 cctttgcccg gagcttctgg accgtgttca tgacatcctt ctgctgtgcc aggaacgcgt
     3601 ccccaagctt ctgcgtccag tccaatttct cattcatcat ggtgaggacc gacggaaagt
     3661 tcacaaggga tttgacgctg gggtcccagt tctgtttctc cagggcggat gtcaactgat
     3721 tccccttaag attggggttc gccttcaccc accgcgccgc ctcaaccacc tcgagggggt
     3781 acgtcgacgc catgagtatc tgggctacga ggtcgtcggg ataaagggcg atgggagcca
     3841 gaagctgctc cagctcctcc tgcttgaatg ccggcttcga cgtccccgta ccctgcgcga
     3901 acacccccac aggaatgaca agggacgcca caagtaccca tatcaatgtc cgtctaaacg
     3961 ctctattcat catactcctc cagatttgta ggaacaaccg acatcaagtt cagatatttc
     4021 tccgtgggca caaagtccct
//
LOCUS       DAOQTR010000149         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25054_length_4035_cov_3.632915, whole genome shotgun sequence.
ACCESSION   DAOQTR010000149 DAOQTR010000000
VERSION     DAOQTR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25054_length_4035_cov_3.632915"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKN85_04770"
     CDS             complement(<1..326)
                     /locus_tag="PKN85_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNT43749.1"
                     /translation="MGVYPREDEGLLTVSELTAELRRFVSGRFRDVRVEGEVSNAKLY
                     PSGHFYFTLKDDLAMIRGVVFNYAARVPSGRVIKDGEQVLCRGRIDVYEKRGEYQLIV
                     SDILASG"
     gene            complement(327..1613)
                     /gene="thiC"
                     /locus_tag="PKN85_04775"
     CDS             complement(327..1613)
                     /gene="thiC"
                     /locus_tag="PKN85_04775"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNT43750.1"
                     /translation="MKNQLIAAREGKITRVMERVARDEGLDKEHLRQHIAEGKATILA
                     NNRHRDFTPKGVGKGLSVKVNANIGTSPERVNIDEEIEKLTVAREAGADAVMDLSTGG
                     NLDEIRKIVIDAAKIPVGTVPIYQAAIETVKKRKAIVRMEADDIFEVIEKHGADGVDF
                     VTVHCGVTKSALERLRKQGRVTDIVSRGGAFLAQWIIVNGKENPLYENFDRLLTIAKK
                     YDMTISLGDGLRPGCIADATDRGQIEELIILGELCAEALDQGVQVMIEGPGHVPINQI
                     ETNMVLQKRLCNEAPFYVLGPIVTDIAPGYDHITSAMGGALAAYYGADFLCYVTPTEH
                     LGLPMPQDVRDGVVVTKIAAHAADLARGSKKAFALDKAMSTYRKALDWKGQIECAIDP
                     EKIRAFRKERNLKNDVCSMCGEFCSMKLMKDYLKKK"
     gene            complement(1621..2487)
                     /locus_tag="PKN85_04780"
     CDS             complement(1621..2487)
                     /locus_tag="PKN85_04780"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNT43751.1"
                     /translation="MKNLVSIAGFDASSGAGVVRDVDTFFSFGFHGIAVPTCTVVQGP
                     GGVERVEPSSEDLFRDVLRAATAGIPVKGVKIGVLCDESHVRATASFLAKRRGAPVVL
                     DPVFAAKNGVPLITDEGRAACIDVLFEKVTVITPNAEEASAITGIKVGTVKAARKAAE
                     KIRSLGPGAVVIKGGHLEGDPVDVLFDGREYLLYRKARFPRSIHGTGCSFSSALACFL
                     AAGYPLSHAFMATQGYLERLFESSYRIDRKGHFYVSSATERNFDTAGQRMKIQGAHGK
                     PPEDPGPLRIWR"
     gene            2879..3346
                     /locus_tag="PKN85_04785"
     CDS             2879..3346
                     /locus_tag="PKN85_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNT43752.1"
                     /translation="MKKILGVIVAVLFIAAASTVFAVGPGGYGPGPKGFGYGRGIDLS
                     KEQMDKMWQIRERFNTDTSSLRYELFQKRGELRTLYSDPKASDAAILAKEKEVDTLRQ
                     KMHDRMVRFKLEQRKVYTPEQLQKLAESGYGPGFGGGRGGRGGGRGLGPCGRF"
     gene            3414..3803
                     /locus_tag="PKN85_04790"
     CDS             3414..3803
                     /locus_tag="PKN85_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HNT43753.1"
                     /translation="MKNIVAVVLLATVTGLFLVSCASEGYNTQKGAAIGAGLGAIAGQ
                     VIGHDTASTLIGAAVGTLVGAVGGNAVDQHVQNQQRTSTQQSAAYPSSSQVTPQDQPP
                     GQWVEVPGQWIGGKWVPAHKVWVPVNP"
     gene            3800..>4035
                     /locus_tag="PKN85_04795"
     CDS             3800..>4035
                     /locus_tag="PKN85_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43754.1"
                     /translation="MTFVVFFLLLCVFITPSLRAQNSYSEFERGLQLTEPQRTQVEDI
                     RNRYINEWQSLRRESSRKQRELRELNRNPETNSA"
BASE COUNT          850 a         1176 c         1142 g          867 t
ORIGIN      
        1 ccgctcgcca ggatgtcgct cactatcagc tggtattcgc cgcgtttctc gtagacgtcg
       61 attcgccccc tgcacaggac ctgttcgccg tcctttatga ccctgcccga ggggacgcgc
      121 gcggcatagt tgaagacaac accgcggatc atggcaaggt cgtccttcag agtaaagtag
      181 aaatgtcccg agggatagag cttcgcgttg gagacctcgc cctcgacacg aacgtcacgg
      241 aaccggccgc tgacgaacct cctgagctcc gccgtcagct ccgataccgt cagaagccct
      301 tcatcctcgc gcgggtagac gcccatctat ttcttcttca ggtagtcctt catgagcttc
      361 atggaacaga actcgccgca catgctgcag acatcattct tgaggttccg ttccttccgg
      421 aatgcccgta tcttttcggg gtctatggcg cactcgatct gccctttcca gtcaagggcc
      481 ttccgatagg tcgacatggc cttgtcaaga gcaaaggcct tcttgctccc gcgcgcgaga
      541 tcggcggcat gggcagctat cttagtaacc acgacaccgt cacggacatc ctgcggcatg
      601 ggcaggccca gatgctcggt gggggtgacg taacagagga aatcggctcc gtagtaggcc
      661 gccagcgctc cccccatggc ggacgtgatg tggtcgtaac ccggcgcgat atcggtgacg
      721 atgggaccaa gaacgtagaa gggcgcctcg ttgcagagcc tcttctgaag aaccatattt
      781 gtctcgatct ggttgatcgg aacgtggccg ggcccttcga tcatgacctg gacaccctgg
      841 tcgagcgctt ccgcgcagag ttcgcccagg attatgagct cctcgatctg accgcgatcg
      901 gtcgcgtccg cgatgcagcc cggacgaaga ccatccccga gactgatcgt catgtcgtat
      961 ttcttcgcga tggtgaggag gcggtcgaaa ttctcgtaga gcgggttctc cttgccgttg
     1021 actatgatcc actgagcaag gaaggcgccg ccgcggctca cgatgtcggt cactctcccc
     1081 tgttttctca ggcgctccag ggcgctcttc gttacaccgc aatgaaccgt cacgaaatcg
     1141 accccatcgg cgccgtgttt ctcaatgacc tcgaagatgt cgtccgcttc catcctgacg
     1201 atcgcctttc tcttctttac ggtctcaatg gcggcctggt atatgggaac ggtccccacg
     1261 gggatcttag cggcatctat gacgatctta cggatctcgt cgaggttgcc tccggtgctg
     1321 aggtccatga ccgcatctgc tcccgcttca cgggcgacgg tcagcttctc gatctcctcg
     1381 tcgatgttca ccctctccgg agaggtgcct atgttggcgt tgaccttgac ggaaaggccc
     1441 ttgccaacac ctttcggggt gaagtccctg tgtcggttgt ttgcgagaat cgtggccttt
     1501 ccctccgcga tgtgctggcg gagatgttcc ttgtctaggc cctcatcgcg ggcgacgcgc
     1561 tccataaccc tggtgatctt tccttcccgt gctgcaataa gttggttttt catggtcgta
     1621 tcatctccat attctcaacg gacccgggtc ttccggcggt ttcccgtgag ccccttgtat
     1681 tttcatgcgt tgccctgccg tgtcgaaatt cctttccgtc gccgaagaaa cgtagaaatg
     1741 cccttttctg tcgatcctgt aactcgattc gaacagcctc tcgagatacc cctgcgttgc
     1801 catgaaggca tgagacaggg gatatcccgc cgcaagaaaa caggcgaggg ccgaagaaaa
     1861 ggaacagccc gtcccgtgga tcgaccgggg aaatctcgcc ttcctgtaga gaaggtattc
     1921 cctgccatcg aacaacacat caacagggtc gccctccagg tgacctcctt ttatgacgac
     1981 ggcaccgggg ccaagagagc gtatcttctc ggctgccttc ctggcggcct tcaccgtccc
     2041 gacctttata cccgtgatgg cggacgcctc ttccgcattg ggcgttatga ccgtgacctt
     2101 ctcgaaaagc acatcgatgc acgctgctct gccctcatcc gtaatgagcg ggacaccgtt
     2161 cttggcggca aacacagggt cgagaacgac gggcgcgccc ctccttttcg ccaggaagct
     2221 cgctgtcgcc ctgacatggg attcgtcgca gagaacgcct atcttcacgc cctttactgg
     2281 aatccccgcc gtggccgccc gcaggacgtc ccggaagaga tcctccgatg atggctcgac
     2341 gcgctccacg ccgccggggc cctggaccac agtgcaggta ggcacggcaa ttccatggaa
     2401 accgaaagag aagaaggtgt cgacatccct cacgacaccc gctcccgaag aggcatcaaa
     2461 tcctgctatg gaaacgaggt ttttcatctt tctcggctcg tatgtacccc ttagattaaa
     2521 actgaatact ataccatagt tagcctcctg aggccataca aatcccgcgg taagaccgct
     2581 tgaatcgctt gaatggccaa aaggtcaagg caaggacatg atcttcactg ttcctggacc
     2641 cgcgaacgat taaaccacga ggaccgttgc tgcgccatgg tgcagggcgt gtattcgacg
     2701 gttttgtcga aggctctctg tgacgtcgac atttgtttcc gtgaaatctt ccggtcatca
     2761 atggtcataa tacatctttc cctacgttta ccgtgagtta cgaacatctt cagcaaatgg
     2821 cacggtaatt gccaaaaagg aagctatgat ttgcaaaaag ccatatgagg aggttacaat
     2881 gaagaagatt ttgggagtta tcgtggcggt cctgttcata gcagcggcat ctactgtctt
     2941 cgccgtcggg ccgggaggtt acggtccggg accaaaaggg ttcgggtacg gccggggtat
     3001 agatctctcc aaggaacaga tggacaagat gtggcagata cgcgagagat tcaacaccga
     3061 cacctcatcc ctgcgttacg agctctttca gaagcggggc gaactgagga ccctctattc
     3121 cgaccccaag gcaagcgatg cggcgatcct ggcaaaggaa aaggaagtcg acactctgag
     3181 gcagaagatg cacgacagga tggtccggtt caaactggag cagaggaagg tctacactcc
     3241 ggagcagctt cagaaactgg ctgagtccgg ttatggtccc ggcttcggcg gcggtcgcgg
     3301 cggtcgcggc ggcgggcggg gactcgggcc ctgtggcaga ttctaggtac atggaaacaa
     3361 ggtgacgggc ctccccttga cggcccgtca cccccatgaa aagggaggga gaaatgaaaa
     3421 atatcgtagc agttgtacta ctggcgacag tgacagggtt gtttttggtc tcatgcgcgt
     3481 ctgaagggta taacacccag aaaggcgccg cgatcggggc cggacttggc gccatagcgg
     3541 gacaggtgat agggcacgat acagcctcga cgctcatcgg cgcggcggtg ggaacccttg
     3601 tcggagccgt gggaggcaac gcggtggacc agcacgtgca gaaccagcag cgcacttcga
     3661 ctcagcagag cgccgcatat ccgtcatcga gccaggtaac cccccaggac cagcctccgg
     3721 ggcagtgggt tgaagtgccc ggtcaatgga taggcgggaa atgggttccc gcacacaagg
     3781 tatgggtgcc cgtgaatcca tgacatttgt cgtattcttc cttctcctgt gcgtgttcat
     3841 aacgccctct ctccgggccc agaactcata ttccgagttc gagagagggc tccagctgac
     3901 ggaaccccag agaacgcagg ttgaggatat caggaacagg tatatcaacg aatggcaatc
     3961 cctgaggagg gaatcgtcgc gaaagcagag ggagctcagg gaactgaacc ggaaccccga
     4021 gaccaattca gccag
//
LOCUS       DAOQTR010000150         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25220_length_4014_cov_3.509977, whole genome shotgun sequence.
ACCESSION   DAOQTR010000150 DAOQTR010000000
VERSION     DAOQTR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25220_length_4014_cov_3.509977"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKN85_04800"
     CDS             complement(<1..798)
                     /locus_tag="PKN85_04800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT43755.1"
                     /translation="MRRFDLRVLLILSLGHLVVDIYQGALPAILPFLKEKLDLTYAMT
                     GMILMGSNFTSSILQPLFGYVSDKRAMAYLLPLGLVAAGAGFSALPFSPYFLLILGLV
                     IISGLGVASYHPEGYKTAHFFTGDKPATGMSVFSVGGNIGFSLGPILSLYIIDLYGFS
                     WLPVIVVPSLLCAAVIVMFRRTIMMPVAVHGEAARKETPKTSSVEYIALGMVILIVVM
                     RSWTQIGLMTYIPFYYIDYLKGDPLYAGKLVFVFLFCGAVGTLLGAPI"
     gene            complement(785..1330)
                     /locus_tag="PKN85_04805"
     CDS             complement(785..1330)
                     /locus_tag="PKN85_04805"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNT43756.1"
                     /translation="MLETIRDRLLTHRPRQIPCDPSLRAATALLLFEQDGDVFCVLTK
                     RTDTVRHHKSEVSFPGGMFEATDRDFEHTALREAEEEVGVRSGDVKVIGRLDDSWTYS
                     GFVISPFVGVMPHPYEFFPNPREVSYLIFLPYSLLKREEFAPRQRGDGWTSFHYNGDR
                     IWGATCRILMTFRDIIENEKI"
     gene            complement(1471..1689)
                     /locus_tag="PKN85_04810"
     CDS             complement(1471..1689)
                     /locus_tag="PKN85_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43757.1"
                     /translation="MKARIDRMSGAHKTRGNINESIRECFHLLAKSCERYGRMERSRA
                     PQVIMDMERLLIWKRIMFLFNADPDRRN"
     gene            1834..2175
                     /locus_tag="PKN85_04815"
     CDS             1834..2175
                     /locus_tag="PKN85_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43758.1"
                     /translation="MKKQKVGTPDLRDKLVTETVKKVMTLLKSKGQEPDKAAVERTTN
                     EAIDDFVAQWYEVSLEAIELRDGEADFVTDDELDDPELIHEAKLRVIKELYEEPPEDF
                     YETIAQSAITS"
     gene            complement(2172..>4014)
                     /locus_tag="PKN85_04820"
     CDS             complement(2172..>4014)
                     /locus_tag="PKN85_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43759.1"
                     /translation="LDAKARKKLEETGRLFQEKLNDVVRILREAEKIVGEMVMRLDRM
                     VALDIVGPMVDAIQKKYGDQEKVVKYLEDVKEDILTHLDDFKATEEAPSPLPFLKIPK
                     AETSFARYAVNVIVNNGESKGAPVVYESNPTYLNLFGRMEYKVQYGMATTDFTMIKAG
                     SLHKANGGYLVIDALELLRNAFSYEALKRALKNGEIRIEDVLEQYRLISTAGLKPEAI
                     HLNVKVVLTGNPYLYYVLHAYDEDSRELFKVKADFDSRMERNRENTDKYALYVAQCQK
                     DENLLPFDRTAVARLVEMGSRFADHQDKLSTRFGDIADLIRESHYWAKKEGMDVVSAS
                     HVTRAVKEKIYRVNRIEERLQEMMLEDTLIVNTAGEKVGQVNGLAVLDIGDYAFGKPS
                     RITAKTYMGRAGIVNLERETKMSGKIHEKAILIITSYLGGKYAVNKPISLSASITFEQ
                     LYEMVEGDSATCAELYALLSSISGVPLKQSFAVTGSMDQNGDVQPIGGVNQKIEGFFH
                     LCKMRGLDGSHGVMIPKRNARNLVLSDDVIEAVEKGLFSIYTIDRMEEGLELLTGMPA
                     GELGADGSYPEGTLNRLVEKRLTEISEAMEKKKGNGEEDREKKDGYY"
BASE COUNT          850 a         1151 c         1133 g          880 t
ORIGIN      
        1 gatgggagct cccagcagcg tgccgacggc accgcaaaag aggaagacaa aaacaagttt
       61 cccggcatag agcgggtctc ccttgagata atcgatgtag tagaagggga tgtatgtcat
      121 gagccctatc tgggtccagg agcgcatgac aacaatgagg atgaccatgc cgagcgcgat
      181 gtattccacc gaagaggtct tcggtgtctc ctttctcgcc gcctccccgt ggacggccac
      241 gggcatcatt atggtcctgc ggaacattac gatcaccgcc gcgcacagga gggagggcac
      301 gacgatgacc ggcagccagg agaacccgta aaggtctatt atgtacaggg aaaggatggg
      361 gcccagagag aaaccgatgt tcccccccac ggaaaagacg gacatgcccg tcgccggctt
      421 gtcccccgtg aagaaatgtg ccgtcttgta accctcggga tgataggatg ccacccccag
      481 gccgcttatt atgacaaggc ccagtatgag gagaaaatag ggagaaaagg gcagcgcgga
      541 aaaacctgca ccggcggcga caagtcccag cggcaggaga taggccatgg cccttttgtc
      601 cgagacatat ccgaacaggg gctgcaggat cgaggatgtg aagttggacc ccatgagtat
      661 catgcccgtc atcgcatagg tgaggtcaag cttctccttc aggaaaggca gaatggcagg
      721 aagcgccccc tggtagatat cgaccacgag gtgaccgaga gacagaatga ggagcaccct
      781 gaggtcaaat cttctcattc tcgatgatgt ccctgaaggt catgaggatc ctgcacgtgg
      841 cgccccatat cctgtcgcca ttgtaatgga aggaggtcca accgtctccc cgctgccgcg
      901 gagcgaactc ctccctctta agcaggctgt agggaaggaa gatgagataa gacacctccc
      961 gggggttggg gaagaactcg tagggatgag gcatgacccc cacaaaagga gagataacga
     1021 atccggaata cgtccatgaa tcgtcaagcc tgccgatgac cttcacatca ccggaccgga
     1081 cgccgacctc ttcctcggcc tccctgaggg ctgtgtgttc gaagtccctg tccgttgcct
     1141 cgaacatgcc tcccgggaac gagacctcac ttttatggtg ccgcacggtg tcggtgcgtt
     1201 tcgtgagcac acagaagacg tccccgtcct gttcaaaaag caggagcgcc gtcgcggccc
     1261 tgagcgaagg gtcgcatggt atctgcctcg gtctgtgggt caggagacgg tccctgatgg
     1321 tttcgagcat ggcctattgt aacgtgaaga cgtccgcaaa agcaacgagg cagcttgcgc
     1381 gcagccggcc ttcgtccggc aattcctcga gggcacgatt cccgcaacgg aggcacctcc
     1441 ggccagtcat cccgtgtcag tattatgaca tcaattccgg cggtcggggt ccgcgttgaa
     1501 gaggaacatg atgcgtttcc agatgagcag cctttccatg tccattatga cttgaggcgc
     1561 cctggatctt tccatccttc cgtatctctc gcagctcttg gcgagaaggt gaaagcattc
     1621 ccggatggat tcgttgatgt tcccgcgggt cttgtgtgct cctgacatcc tgtcgattcg
     1681 ggctttcatg cctacgatca ctgcaagctg tgtgccagat ggcttccgga aaaactattt
     1741 tactccgcgc cgtgttttgt gtaagaatga atgaaagcag ccacgattac ggatacaacg
     1801 acccatgggg cgtctcttcg gagggtttgc gcgatgaaaa aacagaaggt gggaaccccg
     1861 gacctgcggg acaaactcgt gaccgagacc gtcaagaagg tgatgaccct tctcaagagc
     1921 aagggacagg aaccggacaa agccgcagtc gaaagaacga cgaacgaggc catagatgat
     1981 ttcgtcgcac agtggtacga ggtttcactc gaggccatcg aactcaggga cggcgaggcc
     2041 gatttcgtta ccgatgacga gcttgacgac cccgaactca tccatgaggc gaagctcagg
     2101 gtcataaaag aactctacga agaacccccc gaagatttct acgagaccat agcacagagt
     2161 gccattactt cctaataata gccgtctttc ttctcgcggt cttcctctcc gttgcctttc
     2221 tttttctcca tcgcttccga gatctccgta aggcgctttt ccacgagacg gttgagggtg
     2281 ccctcaggat aactcccgtc ggccccgagt tcacctgccg gcatgccggt caggagttca
     2341 agcccctcct ccatgcggtc aatcgtgtaa atggagaaaa ggcctttctc cacggcctcg
     2401 atcacgtcgt cgctcagcac caggttccgt gcgttgcgtt tcgggatcat cactccatgt
     2461 gatccgtcga gaccacgcat cttgcagagg tggaagaagc cctctatctt ctggttcacc
     2521 ccgcctatgg gttgcacgtc cccgttctgg tccatggagc ctgtcacggc gaaggactgc
     2581 ttcagcggaa cgccgctgat ggatgaaagc agcgcgtaca gctcggcaca ggtagcgctg
     2641 tcaccttcga ccatctcgta gagctgctcg aaggtgatcg aagcggagag gcttatgggc
     2701 ttattgaccg cgtatttgcc gccgaggtag ctcgtgatga tgaggatggc cttctcgtgg
     2761 atcttgccgc tcatcttcgt ctcgcgctcc aggttgacga tcccggccct gcccatgtag
     2821 gtctttgcgg tgatacggga tggtttgccg aaggcgtagt cgccgatatc aaggacggcg
     2881 aggccattca cctgtcccac cttctcgcct gcggtgttca cgataagagt atcctcaagc
     2941 atcatctcct gcagtctttc ctcgatgcgg ttcaccctgt agatcttctc cttgacggcc
     3001 cgcgtcacgt gcgaggcgct cacgacatcc atcccttcct tctttgccca gtaatgagat
     3061 tcccgtatga ggtcggcaat gtcgccgaag cgtgtcgaga gcttatcctg gtggtcggca
     3121 aaacgcgagc ccatctccac gagccgcgcc acagccgtcc tgtcgaaggg gagaaggttc
     3181 tcatctttct gacactgggc tacgtacagc gcgtacttgt ccgtgttttc ccggttcctt
     3241 tccatccggc tgtcgaaatc ggccttcacc ttgaaaagct cccgggagtc ctcgtcgtag
     3301 gcatggagaa cgtagtagag ataggggttg cccgtcagga cgaccttcac attgaggtgg
     3361 atcgcctcgg gcttcaaacc tgccgtgctg atgaggcggt attgctccag aacgtcctcg
     3421 atgcggatct ccccgttctt gagggcccgc ttcaatgcct cgtaagagaa ggcgtttctc
     3481 aggagttcaa gggcgtcgat gacgagatag cccccgttcg ccttgtgcag tgagccggcc
     3541 tttatcatcg tgaagtccgt cgtcgccatg ccgtactgta ctttgtactc catcctgccg
     3601 aagaggttga gataggtcgg gttgctctcg tacaccacgg gggctccctt gctctccccg
     3661 ttgttgacta tgacattgac ggcatacctg gcgaaagagg tctccgcctt cgggatcttg
     3721 aggaagggca gcggcgaagg agcctcctcg gtggccttga aatcatcgag atgagtcaat
     3781 atgtcctctt tcacgtcctc gaggtacttg acgaccttct cctggtcccc gtacttcttc
     3841 tggatggcat cgaccatggg gccgacgata tcgagggcga ccatcctgtc aaggcgcatc
     3901 accatttcgc cgacgatctt ctccgcctcc ctgaggatac ggaccacatc gttcagtttc
     3961 tcctggaaga gacgccccgt ttcttcgagc ttcttcctcg ccttggcgtc gagg
//
LOCUS       DAOQTR010000151         4004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25327_length_4004_cov_4.113700, whole genome shotgun sequence.
ACCESSION   DAOQTR010000151 DAOQTR010000000
VERSION     DAOQTR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25327_length_4004_cov_4.113700"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKN85_04825"
     CDS             complement(<1..363)
                     /locus_tag="PKN85_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43760.1"
                     /translation="MMIAETRTVTSTSRTSATTEDITIRETTTSRLVFRPMLVVNDRQ
                     PDAAVKGTFVYQRKGATQSWEDVPLPPLSSLKKDEAVKLELKSEELLRLFNNLSTLYE
                     LFRTQGISRGESKFIKANA"
     gene            complement(710..2080)
                     /locus_tag="PKN85_04830"
     CDS             complement(710..2080)
                     /locus_tag="PKN85_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNT43761.1"
                     /translation="MERKNILCIGAGYVGGPTMAMIAYKCPQYRVTVVDINHEKIERW
                     NSDDLPVYEPGLDEIIRATRGKNLFFETDIDKGIRESEIIFVGVNTPTKTFGAGAGKA
                     ADLQYWEKTARQIREASESPKIVVEKCTIPVKTALAMERILTSKDGDISFDVLSNPEF
                     LAEGTAMRDLEFPDRVLIGSRETPSGIEARKAVVDIYLNWVPRERIIESNIWSSELSK
                     LVANAFLAQRISSINAVSALCEKTEADVAEVSRAIGLDSRLGPKFLNASVGFGGSCFK
                     KDILNLAYICRSYGLEDVADYWESVVDINEYQKERFILGILSAMFNTLAGKRVCLFGF
                     AFKADTGDTRETPAFTIARRLVEEKADLVITDPKALANAKIDLADVEGITYEPDPYKA
                     AHGADAIAVMTEWKLYTELDYKRIYDSMAKPAFIFDGRNILNHRLLHDIGFNVYPIGK
                     PPLVHF"
     gene            complement(2172..>4004)
                     /locus_tag="PKN85_04835"
     CDS             complement(2172..>4004)
                     /locus_tag="PKN85_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43762.1"
                     /translation="MIRRVEDRTNIDRTKDGKKTKDQLTEELLSLRKRVAELEGPGAG
                     ARSRPEVPSGLEERYRTLFEHASDAIIVADPEGNFLEVNRKAEDLLGYGKEELLRMKV
                     TDIHPREELDRVVLHFSGAVAGRIDSLHDTRLLRKDGTVVPADITGCFVEYEGKRLAQ
                     GIFRNIAERKEMEETLRKSEEQLRVLSENLADGMVYQINSGADGRQRVFTYVSPAVER
                     FHGVKVEDAMMDPSLIYDQVGAEYRTALKESETRACATMSRFEIDVPMRLPSGDVRWR
                     RLISFPHLHSDGSVMWDGIELDVTERRKMEEELAAHRDHLGKLVEERTARISEEVARR
                     KEKEEQYLSLVESIKEWVWEMNADLVHTYVSPRIREILGYEPEEFIGRAPFDFMAPGE
                     PERIAPLAKKILSGKEQFTSLGTAALHKNGQVVYLEASGRPFFDDKGTFLGYRGSCHD
                     ITDQKKTMDALRERERELTAKSEALEEVNAALKVLLRQREEDREELEARLVSNIREMV
                     LPYIHKMRKDKLDLRHRTFLDIVAANLNEIMSPFPTTIKQLNFTPREIEVASLIKEGR
                     TTKEIAEFLGIATSAVDSHRNNIRTKLGLNNKKINLRSHLLSLG"
BASE COUNT          722 a         1165 c         1127 g          990 t
ORIGIN      
        1 ggcattggcc ttaataaact tcgattcacc tctggaaatg ccctgtgttc ggaaaagctc
       61 gtagagggtc gagagattgt taaacaagcg caacaattct tcggatttca gctcaagctt
      121 gacggcttca tctttcttta gacttgatag aggaggaagg ggtacatctt cccaactctg
      181 cgttgcccct ttgcgttgat acacgaaagt tccctttacg gccgcgtctg gctggcggtc
      241 attgaccacc agcatgggtc ggaagaccaa ccgactggta gtggtctcac gaatagtgat
      301 gtcttctgtt gtagctgatg tccttgacgt ggaggtgacc gttctcgttt cagctatcat
      361 catggtctct tcatgcaatg aaacatgatt gaaactgccc catcagacag ttctttgccg
      421 cagaggagag gaacagggcg ctattggata ttcccaccgt attctatccg tgccctttga
      481 tgaggtttat tctattctgt tactgtcctc aggaaatgtc aacgcaaaaa cacccgatgg
      541 agcggatggg ttcgaggcaa tgagcaagag aacgcaatat taacagtccg accggctcga
      601 atggcttgat cgctttaacc aaacaaagaa agagtgtacg gtttctctct gtgtgctctc
      661 ctcaaaacct ctgtgtcctc gagcgaagcg ggcgtgagat gtctttgcct tagaagtgca
      721 ccagcggcgg tttgcctatg gggtagacgt tgaagccgat gtcgtgcaat agcctgtggt
      781 tgaggatgtt acggccgtcg aagatgaagg ccggttttgc catggaatcg tagatgcgct
      841 tgtagtcgag ttcggtgtag agcttccatt ccgtcatgac ggctatggcg tcggcgccgt
      901 gggcggcctt gtaggggtcg ggttcgtagg ttatcccttc gacgtcggcg aggtcgatct
      961 tcgcgttggc aagggccttg gggtcggtga tgacgaggtc ggccttttcc tcgacgagcc
     1021 ttctcgctat ggtgaacgcc ggggtctcgc gggtgtcgcc cgtgtcggcc ttgaaggcga
     1081 agccgaagag gcagacccgt ttccccgcca gcgtgttgaa catggcgctc aagatgccca
     1141 ggatgaagcg ctccttctgg tattcgttga tgtcgacgac actctcccag taatcggcga
     1201 cgtcctcgag gccgtaggac cggcatatgt aggcgaggtt gagaatgtcc ttcttgaagc
     1261 aggaaccgcc gaaccccacg ctcgcgttga ggaacttggg tcccaggcgg ctgtcgagtc
     1321 cgatcgcccg cgatacctcg gccacatcgg cctccgtctt ctcgcacagc gccgagacgg
     1381 cgttgatgga tgatatcctc tgggcgagga aggcgttggc gacgagcttc gagagttcgc
     1441 tgctccagat gttgctctcg atgatcctct cccgcggcac ccagttgaga tagatatcga
     1501 caacggcctt gcgcgcctct attccgcttg gtgtctcacg ggagcctatg aggacgcggt
     1561 cggggaactc gaggtcccgc atcgccgtcc cttccgccag gaactcaggg ttggagagga
     1621 catcgaaact gatatcgcca tccttggatg tcaggatacg ctccatcgcg agggccgtct
     1681 tgacgggaat cgtacatttc tcgacgacga tcttcggcga ctcggaggcc tccctgatct
     1741 gccgcgccgt cttctcccag tactggaggt ccgcagcctt tcccgcgccg gcgccgaagg
     1801 tcttggtagg agtgttcacc cccacgaaga tgatctccga ttccctgatg cctttatcga
     1861 tgtcggtctc gaagaagagg ttcttccccc gcgtcgcccg tatgatctca tcaagcccgg
     1921 gctcataaac cggcaggtca tcggagttcc agcgctcgat cttttcatgg ttgatatcga
     1981 ccacggtgac cctgtactgc gggcatttgt aggcgatcat ggccatcgtc ggcccgccga
     2041 cataaccggc accgatgcac agaatattct tacgctccaa ggagaccctc catcgatagt
     2101 tttgggtatt ctaccacaaa cacggtgttg cggggaagaa actccgtgat atctttcccc
     2161 ctttcgtccg cttatccgag ggagaggagg tgcgagcgga ggttgatctt cttgttgttc
     2221 aggccgagct tggtccgtat gttgttcctg tgggaatcga cggcgcttgt cgctatcccg
     2281 aggaattccg caatctcctt ggtcgtcctg ccttccttga tgagcgaggc cacctcgatc
     2341 tccctggggg tgaagttcaa ctgctttatc gtcgtcggga agggtgacat tatctcgttc
     2401 aggttcgccg cgacgatatc gaggaacgtc ctgtgcctca agtccagctt gtccttccgc
     2461 atcttgtgta tgtagggcag gaccatttcc cggatgttgg ataccagtct cgcctccagt
     2521 tcctcccggt cctcctcgcg ctgcctcaac aggaccttga gggcggcatt cacctcctcg
     2581 agggcctccg atttggccgt cagttcccgt tcgcgttccc tgagggcatc catcgtcttc
     2641 ttctggtcgg tgatgtcatg gcaactcccc cggtatccga ggaaggtgcc tttgtcatcg
     2701 aagaaagggc gcccactcgc ctcaaggtag accacctgcc cgttcttgtg aagggccgcg
     2761 gttcccaggg acgtgaactg ttctttgcct gacaggatct ttttcgccag gggagcgatc
     2821 ctttccggtt cgccgggggc catgaaatcg aagggggctc tgcctatgaa ctcctctggc
     2881 tcatagccga ggatttctcg tatccggggg cttacgtacg tgtggacgag gtccgcgttc
     2941 atttcccaca cccattcctt tatcgattcc acgagggaga ggtattgctc ctctttctct
     3001 ttcctcctgg caacctcctc actgatccgg gcggtgcgtt cttccaccag tttgccgaga
     3061 tggtcgcggt gcgccgccaa ctcctcttcc atcttcctgc gctcggtgac gtcgagttca
     3121 atgccgtccc acatgacgct cccatcggaa tgaaggtggg gaaaggagat aagacgccgc
     3181 cagcggacat cgcccgaagg aaggcgcatc ggcacatcga tctcaaaccg tgacatcgtc
     3241 gcgcaggcgc gggtttcgga ttccttgagc gccgtccggt actcagcgcc cacctggtcg
     3301 tagatgagtg aagggtccat catggcatcc tccaccttca caccgtgaaa cctctccacc
     3361 gccgggctca cgtatgtgaa cacccgctgc cttccgtcgg caccgctgtt gatctggtag
     3421 accatcccgt cggcgaggtt ctcgctgagg acgcgcaatt gctcctcgct tttccgcagg
     3481 gtttcctcca tctccttgcg ctcggcgatg tttcggaaga tcccctgggc caaccgcttt
     3541 ccttcatatt ccacgaagca tcccgtgatg tccgcgggaa ccaccgtgcc gtctttccgc
     3601 agtagcctgg tgtcgtgaag agagtcgatc cggcccgcca ccgctcccga gaagtgaagg
     3661 accaccctgt cgagttcttc ccgcgggtga atatccgtca ctttcatgcg cagcagttct
     3721 tccttcccgt agcccagaag gtcctccgcc tttctgttga cctcaaggaa gttcccttcg
     3781 gggtcggcaa cgattatcgc gtcgctcgcg tgctcgaaga gggtgcgata tctttcctca
     3841 agccccgagg gcacctcagg gcgggacctc gcgcctgcgc ccggtccctc cagttcggca
     3901 acccttttgc gaagggagag gagttcttcg gtgagttggt ctttcgtctt ctttccgtct
     3961 ttcgtgcgat cgatgtttgt gcggtcctcg actctacgta tcat
//
LOCUS       DAOQTR010000152         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25370_length_3999_cov_3.649594, whole genome shotgun sequence.
ACCESSION   DAOQTR010000152 DAOQTR010000000
VERSION     DAOQTR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25370_length_3999_cov_3.649594"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKN85_04840"
     CDS             complement(<1..332)
                     /locus_tag="PKN85_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43763.1"
                     /translation="MATDDIKTVRMAEVVRPLKTTFATSLGRKDAMRSVIVEVRLMDG
                     SSGFGECATSFVLPHETVDRIRAVIRAERPHLTGKRAADFSAIAAELRERHPAFPMTV
                     SGLETTIW"
     gene            complement(319..1305)
                     /locus_tag="PKN85_04845"
     CDS             complement(319..1305)
                     /locus_tag="PKN85_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNT43764.1"
                     /translation="MEERLKKKPDPSEGKLLSVVASVADLLAGPSATAERLTQILLNE
                     RLRCLGDEDDWYMVEALEQQKLSDEGWRGYPGWIGKNRAVPLEGRPALDGVLVRGTLA
                     SAPSLPALYLSAGTRVEMTGEHSGVLVGIRLPGGMMAWVRGADIRHTGTGGDPAAKRQ
                     RIIEAAMSFIDTPYLWGGRSTSLHSLRSGLPVSAVGYALPATGVDCSGLINLSYRAAG
                     IDVPRDARDQHAAARPLPPGRLQPADLIFLTPAEGRGIDHVILYLGGERFVEAVETGR
                     PVEVGTFRTRFGHSREEMVRDGLSDWSGRIHFASLLEEGGNRKNGSEAYGDR"
     gene            complement(1305..1676)
                     /locus_tag="PKN85_04850"
     CDS             complement(1305..1676)
                     /locus_tag="PKN85_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HNT43765.1"
                     /translation="MKIRITAGPVSVEAELGTTPCAMAIAGVLPVTASPNEWGDEFYF
                     EVPVHMPLDDTATRVVKVGDIGYWPPGDAIAIFFGPTPLSTGQNPVPASEVNVVGRIT
                     GDPALLKKARGSVSIRLEPAT"
     gene            1957..>3999
                     /locus_tag="PKN85_04855"
     CDS             1957..>3999
                     /locus_tag="PKN85_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43766.1"
                     /translation="MVGQWDSFLKATPQPILVLDKEFIIVAANKRSQEIAGATEGELI
                     GKRCYEVMHGTKGPAERCPMRSLLTRGNLETVEMEIETIHGTFLVSCAPIVDASGALL
                     GVVHVATDITEKKKAEEELREREVRYRYLFENSPVGMFRSSLADGSIVDANLTLSRMF
                     GIDESEDLHAEDFYADPRDRQRLLNAIKRKGVANDEVLMSRRDGSLFWALISARLDIR
                     EHYIEGVVTDITAEKEAREALRESEERYRVAIENSNDCVAIFRDGVYVYANQKYVEAL
                     GVRDAGDILGTGIGRFIHPDDYDRIERYLRERKAGKDVPTRYELRIIDVRGKVVFFEA
                     AVSVINLKGEPATLVFMRDITKRIEVQKKLEEQVEFLQNFIDTMPNPIFIKDPKGRFI
                     QCNRAFEEYYGVSRQAVEGKTVFDIAPAEEAKVHAGKDRELMKKGGVLEYDGSVTDPS
                     GCAHDVIIRKAAFHKPDGSPGGIVGVVIDVTELKRAGEMLKESEAKYRTVAEESLAGF
                     YIIQDGLFRYVNRRFCEITGYSYDEIVDRMEPLDLVYGEDRDVVEENLRKRLGSEAGS
                     IQYRFRMTRKSGLIARVEALGSSFDYRGRPAAIGTLLDITKETILEEQLQQAQKMEAV
                     GQLAGGMAHDFNNILTTIMGYCGLLQTELDEDDPRQLYAEEISMASEKGAQLTSGLL"
BASE COUNT          835 a         1140 c         1271 g          753 t
ORIGIN      
        1 ctccagatgg ttgtctccaa cccggaaacg gtcatgggaa atgcggggtg tctctcgcgc
       61 aactctgccg cgatggcgga gaaatcggcc gcccttttcc ccgtcagatg gggcctctcc
      121 gcgcggatga ccgcccgtat cctgtccacc gtctcgtgag ggaggacgaa gcttgtggcg
      181 cattccccga atcctgagga cccatccatg agacggacct cgacgatgac actccgcatc
      241 gcgtccttac gccccaggga ggttgcaaag gtggtcttca ggggacggac cacttccgcc
      301 atgcggacgg tctttatgtc atcggtcgcc ataggcctcc gatccgttct ttctgtttcc
      361 tccttcttcc aggagggaag cgaagtggat gcgcccggac cagtcggaga ggccatccct
      421 caccatctct tcacggctgt gtccgaaacg tgttcggaaa gtccccacct cgacaggtct
      481 gcccgtctca actgcctcga cgaaccgttc tccgccgagg tagaggatca catggtcgat
      541 cccccggcct tcagcggggg tgaggaatat gagatcggca ggctggagcc ggcccggggg
      601 aaggggtctt gccgccgcat gctggtcgcg ggcatcgcgg ggaacatcga tcccggcggc
      661 ccgataggag aggttgatga ggccggaaca atccaccccc gttgcgggga gggcgtaccc
      721 gacagcggaa accggaagcc cgctgcggag gctatgcaaa gacgtgctcc gtcctcccca
      781 gagatagggg gtgtcgatga aggacatggc tgcttcgatg atcctctgcc tctttgcggc
      841 ggggtcgccg ccggtgccgg tgtgccggat gtccgcaccc cggacccacg ccatcatgcc
      901 gccaggcagg cgtatgccga ccaggacgcc actgtgttct cccgtcatct ccaccctggt
      961 tcccgcagaa agatagagag cgggcaggga aggtgcggaa gcaagcgtgc cgcgcacgag
     1021 gacgccgtcc agagcgggtc tgccctcaag ggggaccgcc cggttctttc ctatccatcc
     1081 gggatagccc ctccaaccct catcggagag tttttgctgc tcgagggcct ccaccatgta
     1141 ccagtcgtcc tcatcgccga ggcagcgcaa tctctcgttg aggaggatct gtgtcagccg
     1201 ctctgccgtg gcggatggtc cggcaagcag gtcggcgacg gacgccacga cggacaggag
     1261 cttaccttcc gaagggtccg gcttcttttt gaggcgttct tccattacgt cgccggctca
     1321 agccgtatgg aaacggagcc cctggccttc ttgagaagcg ccgggtcgcc ggttatcctg
     1381 ccgacgacat tgacctcgct cgcgggcaca gggttttgtc cggtcgagag cggtgtgggg
     1441 ccgaaaaaga tagcgatggc gtctcccggc ggccagtatc cgatatctcc cacctttacc
     1501 acgcgtgtgg cggtatcgtc gagtggcatg tggaccggta cctcaaagta gaactcgtcg
     1561 ccccattcat tcggggatgc cgttacgggc agaaccccgg caatggccat ggcacagggc
     1621 gtggtgccaa gctcggcttc aacggagaca ggacctgctg tgatacggat cttcatgacg
     1681 cgcactcctt tcggtctact ttatcctcct ttcctgtccc gtgtcaaaag atgggtcgcg
     1741 ccggcaagcc cttcgcgttt ctcttgacct gagacgggaa tggtgtatca ttgaagaaat
     1801 caggaccatc agacacgatg aaaacaggtg agcatgaaaa agaggtcgcc tgcgcagccc
     1861 gacacttcgc caccgcgccg cacgagatct ggatctgcgg ctgacgtccg cgccggaggg
     1921 aagaacagat tctccgcggg gacaagtctt gcgcgcatgg tcggacaatg ggacagcttc
     1981 ctcaaggcga cgccccaacc catcctggtc ctcgacaaag aattcatcat cgtcgcagcc
     2041 aacaagagat cgcaggagat agcgggcgcc actgaagggg agctcatcgg gaaaagatgc
     2101 tacgaggtca tgcatggcac gaaaggtccc gccgagcgat gccccatgcg ttcgcttctc
     2161 acccggggga accttgagac cgtggaaatg gagatagaga cgatacacgg gacgttcctt
     2221 gtctcctgcg cgcccatcgt ggacgcgtcg ggtgctctcc tgggggttgt ccacgtggcg
     2281 acggatatca cggagaagaa gaaagcagag gaagagctga gggaaaggga ggtgaggtac
     2341 cgttatcttt ttgagaactc tcctgtcggc atgttcagga gctccctggc ggacggaagc
     2401 atcgtcgatg ccaacctgac cctgtcgagg atgttcggga tcgatgaaag tgaggacctg
     2461 catgcggagg acttctatgc cgatccccgg gaccgtcagc gactcctgaa cgcgatcaag
     2521 cgcaaggggg ttgccaacga cgaggtcctg atgtcgaggc gggatggaag cttgttctgg
     2581 gctctcattt cggcccgtct cgacatcagg gagcattaca tcgaaggcgt cgtgacggac
     2641 atcaccgcgg agaaggaggc gcgggaggcc ctgcgcgaga gcgaggagcg ataccgcgtc
     2701 gccatagaga actccaatga ttgcgttgcc atattcaggg atggcgtcta tgtctacgcc
     2761 aatcaaaagt acgtcgaggc gttgggggtc cgtgatgccg gggatatcct gggaacgggc
     2821 atcggacggt tcatccatcc agacgactat gaccgcatag aacgctatct ccgggagcgc
     2881 aaggcgggca aggatgttcc cacccggtac gagctgcgga taatcgatgt gaggggaaag
     2941 gtcgtattct tcgaggcggc agtttcagtg ataaacctca agggtgagcc tgcgaccctt
     3001 gttttcatgc gggacatcac gaaacggata gaggtccaga agaagctgga ggagcaggtg
     3061 gaattcctcc agaacttcat cgacaccatg ccgaacccca tcttcataaa ggacccgaaa
     3121 ggaagattca tccagtgcaa cagggccttt gaggaatact acggtgtgtc gcgccaggcg
     3181 gtggagggta agaccgtctt cgacatcgct cccgcggagg aggcgaaggt ccatgccggc
     3241 aaggacaggg aactgatgaa gaaagggggc gtgctcgagt atgacgggtc ggtcaccgac
     3301 ccttcggggt gcgcccatga cgtcataatc cggaaggccg ccttccacaa gcctgacggc
     3361 tctcccggag gtattgtcgg cgtcgtcatc gacgtaacgg aacttaagag ggccggagag
     3421 atgcttaagg agtcggaggc aaagtaccgc accgtcgccg aggagtccct ggccggcttc
     3481 tatatcatcc aggacggtct cttccggtac gtgaacagga ggttctgcga gatcacgggt
     3541 tatagctatg atgagatcgt tgacaggatg gagcctctcg atcttgtgta cggagaagac
     3601 cgggatgttg tggaagagaa cctgaggaag cgccttggta gtgaggcggg gagtatccag
     3661 tacaggttcc ggatgacgcg caagagcggc ctcatcgcca gggtggaggc gctggggagt
     3721 tcattcgact atcggggcag accggccgcc atcgggaccc tcctcgatat cacgaaggag
     3781 accatactcg aggagcagct tcagcaggcc cagaaaatgg aggccgtcgg acagctggcc
     3841 gggggcatgg ctcacgactt caacaatatc ctcaccacca tcatgggtta ctgcggtctc
     3901 ctccagaccg agctggatga agatgatccc cgtcagctct atgcagagga gatcagcatg
     3961 gcatccgaga agggagcgca gctcaccagc ggtcttctt
//
LOCUS       DAOQTR010000153         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25396_length_3997_cov_3.869609, whole genome shotgun sequence.
ACCESSION   DAOQTR010000153 DAOQTR010000000
VERSION     DAOQTR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25396_length_3997_cov_3.869609"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..225
                     /locus_tag="PKN85_04860"
     CDS             70..225
                     /locus_tag="PKN85_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43767.1"
                     /translation="MKRSGIFCFCLLMLAAVIISETADAQPVKVSPVPKIARKKLTAD
                     LVVSMSG"
     gene            255..962
                     /locus_tag="PKN85_04865"
     CDS             255..962
                     /locus_tag="PKN85_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43768.1"
                     /translation="MITIEVTVKNAGPGALPKVCSLAMDLGNMDTHPDYTNRIIPWYA
                     NNIPALAPGAEVKISKTITIPYPGTYKLGIIIITEGLQAGDENPVNNRHQMTFVASKP
                     PDASDLVLDSVALNQEGRIVLRMHNSDDPIPDQDFHSSYVRVKVDNGLNKTIFLRDMD
                     PSGILKKGSTLPSGGAFPGGGTTHLIFAWPNSGPDGIKLDPGHAYIVEVTLDYNGRIR
                     DSNRSNNKRTVTLTYNP"
     gene            complement(1071..2183)
                     /locus_tag="PKN85_04870"
     CDS             complement(1071..2183)
                     /locus_tag="PKN85_04870"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT43769.1"
                     /translation="MTKGPGSLEVVLEPYLDKYDLPALGAAVVRDGKILAMGVVGTRA
                     AGQQIPVTTGDRFHIGSDTKAMTALLAAIYVDAGKLRWNSTVEEVFPELKKNLAAGWG
                     QVSLEGLLSHTSGIPADDERFGKLLEGSLEQGDLDLRELRYWMLSRFLTQPVAAAPRK
                     QFAYSNMGYMIAGAMIERAVGRSWEELMLEKVFVPLRLRSAGFGPQSTVGTVDAPLPH
                     KIVDGKAKPMLAGPNADNPLVLGPAGTVHMSLQDFARWAIWNLGKGKRGPGLVRSETL
                     AKLTTPVIDIPPGKDAAPGTPSGGRYALGWGEREYSWARGPFLTHAGSNGMNLAHIIL
                     DLKTDFGMVLMTNIGGKKADEALRGLAEELYRRYGK"
     gene            2496..3497
                     /locus_tag="PKN85_04875"
     CDS             2496..3497
                     /locus_tag="PKN85_04875"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNT43770.1"
                     /translation="MQLEQTFMNFLLRHQERHNRTYHFLILMDAIISAAKRIEHYYIT
                     GALKGNLGCTDRVNVQGEDVMQMDDIAHEIIIHYLKTTGRVIRAVSEESKDIIPLDEE
                     GGRYFIYFDPLDGSSNVAHGLPVGFLFGIAKRNLDGPEDFHLRAGKDYIAAGMFVIPT
                     GSLTIAFREAGTWRFHIDETMNYVKPVRIMLPDTPDTWELSFNATNRYTYRKEVREWV
                     RRNERRYTFRYLGALAGDFHRILTRGGMFMYPAIVNHPDPKKLRPEGKLRLMYEASVV
                     AFMCREAGGHAVDERGTPILDIEPNDHHQRTALYVGSKPLVEDIAKALRADGHTGLR"
     gene            3682..>3997
                     /locus_tag="PKN85_04880"
     CDS             3682..>3997
                     /locus_tag="PKN85_04880"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HNT43771.1"
                     /translation="MKRRAFTVRRSNRPVKKEITGSIGDIVKEITRDRNVKNEQYREQ
                     SLKMHGLMCARCGREFDHTNRHLLTVHHRDGNHNNNPPDGSNWENLCVYCHDDEHGRG
                     LLG"
BASE COUNT          939 a         1192 c         1096 g          770 t
ORIGIN      
        1 tgacggcgac acaaaagacg gtgagaattg agaacaagaa cactaagaac aagaacagag
       61 ggggacgata tgaaacgatc cggaatcttc tgcttctgcc tcttgatgct cgccgccgtc
      121 attatttccg agaccgctga tgcgcagccg gtcaaggtgt caccggtccc gaagatagcg
      181 cggaagaaac tgacggccga cctggtggtg tcaatgtcag gataaccccg gagcatccga
      241 gagccaggaa agacatgatc accattgagg tcaccgtaaa gaatgccggt cccggagcac
      301 tcccgaaggt ctgttctctc gccatggacc tcgggaacat ggacacacat cccgactaca
      361 ccaaccgcat aataccgtgg tacgcaaaca acattccagc cctggctccc ggcgcggaag
      421 tgaagatatc aaagaccatc acgataccct atccgggcac ctataagctt ggtattatca
      481 tcatcaccga aggcttgcag gcgggtgatg agaatcccgt caacaaccgg catcaaatga
      541 cctttgtggc ctcgaaaccc cctgacgctt cggaccttgt ccttgacagc gtggcgctga
      601 accaggaagg gcggatcgtg ctcaggatgc acaacagcga tgaccccatt cctgatcagg
      661 atttccacag ctcctatgtc cgggtaaagg tcgacaacgg actgaacaaa acgatattcc
      721 tcagggacat ggacccctcg ggtatcttga agaaaggcag cacccttcct tcgggcggag
      781 cattcccggg tggaggtacg actcatctca tcttcgcctg gccaaactcg ggacccgacg
      841 ggatcaagct tgatcccggg cacgcctaca tagtggaggt cacccttgat tacaacggcc
      901 gcatcaggga ctcgaacaga tcaaacaaca agagaacggt aacgctgacc tataacccgt
      961 aagaacagct cccgggtttc gcggaaaggg ccggtacctg caccctcctt cttcctctgg
     1021 tctttgaccc gcaacgcgca acccggaacc cgcaacggtc tttcggttgc ctacttcccg
     1081 tatctcctgt agagttcctc ggccaggcct cttaaggcct cgtcggcctt cttgccgccg
     1141 atgttcgtca taaggaccat cccgaaatcg gtctttagat caaggatgat atgggcaagg
     1201 ttcatgccgt tcgatccggc gtgagtgaga aaggggcctc ttgcccagga atattccctt
     1261 tccccccagc caagcgcgta acgtcccccc gagggcgtcc cgggggcggc gtccttcccc
     1321 gggggaatgt cgattacggg ggtagtcaat ttggcgagcg tttcggaacg gacaagaccg
     1381 gggccgcgtt tgccttttcc caggttccag atggcccacc tggcaaaatc ctgcagggac
     1441 atgtgcactg tgccggccgg tcccagaacg agagggttgt cggcgttcgg cccggcaagc
     1501 atgggctttg ccttgccgtc aacgatcttg tgcgggagcg gggcgtccac cgttcccacc
     1561 gtcgactgag gaccgaaacc ggcgctgcga agcctcaagg ggacgaatac cttctcgagc
     1621 ataagctctt cccacgatct gccgaccgca cgttctatca tggccccggc tatcatgtac
     1681 cccatgttgg aatacgcgaa ctgcttccgg ggcgcggccg ccacaggttg cgtcaggaac
     1741 ctcgacaaca tccagtagcg cagttccctg aggtccagat ccccctgttc aagggaccct
     1801 tcgaggagct tgccgaatct ttcatcatcg gcaggtatcc cgctggtatg ggaaaggagt
     1861 ccctccagcg acacctggcc ccagccggca gcgaggtttt ttttcaactc cgggaatacc
     1921 tcctccaccg tcgaattcca gcgaagcttg cccgcgtcca cgtatatcgc cgcaaggaga
     1981 gccgtcattg ccttcgtatc ggagccgatg tgaaaacggt cccccgtcgt caccgggatc
     2041 tgttgtcctg ccgcccttgt gccgaccacc cccatcgcca ggatcttccc gtccctgaca
     2101 acggcagcgc caagcgccgg cagatcgtat ttgtcgaggt acggttcgag aacgacttcc
     2161 agtgagccag ggcctttcgt catcttcggc ttctcataca ggaccctctg ggcacgaacg
     2221 tccccggccg tcaggatcaa aagaacggcg atgcaaacga agcaacacag cataaacggt
     2281 gcctgaccct ttttcatcat cgaacaaccc cctttcgttg ggacgaattc cggtgaccac
     2341 tcttccggac agaacgataa tagccaacta tgaccaaggg ttcaaggata aagacggttt
     2401 ttctgcttcg ttatcttttg acttgaccgc ccgaaagcac ttatacttca caggtatctg
     2461 ccgcatccgg caaattacgg caaaaagagg tacgcatgca actggaacag acattcatga
     2521 attttctgct tcgccaccag gaaaggcaca atcggaccta tcacttcctc atccttatgg
     2581 acgccatcat cagcgcggca aagcgtatcg agcattacta tatcacgggt gccctgaagg
     2641 gaaacctcgg gtgcaccgac agggtgaacg tccagggaga agacgtgatg cagatggatg
     2701 atatcgccca cgagatcatc atccattacc tgaagacgac aggccgcgtc atacgcgccg
     2761 tcagtgagga atcgaaggac atcatccctc tcgacgagga aggcgggcga tacttcatct
     2821 atttcgatcc tctcgacggt tcgtccaacg ttgcccacgg gcttcctgtg ggttttctct
     2881 tcgggatagc gaagcgcaac ctcgacggtc ccgaggactt tcacctgagg gcgggaaagg
     2941 attatatcgc cgccggcatg ttcgttatcc ccaccgggtc cctgacgatc gccttccggg
     3001 aggctggtac ctggcgcttc catatcgacg agaccatgaa ctatgtcaaa ccagtcagga
     3061 taatgctgcc cgataccccg gacacctggg agctctcctt caatgccacg aaccgctaca
     3121 cgtaccggaa agaggttcgt gaatgggtgc gacggaatga aagacggtac actttccgct
     3181 atctcggggc ccttgcgggc gacttccacc gcatcctcac gcggggaggg atgttcatgt
     3241 atcccgccat cgtcaaccac cccgacccga agaagctaag gcccgaaggc aagcttcgcc
     3301 tcatgtacga ggcatccgtg gtggccttca tgtgcaggga ggcgggaggt cacgcggtgg
     3361 acgagcgcgg caccccgatc ctcgacatag agccgaatga ccaccaccaa agaacggccc
     3421 tctacgtcgg ttccaaaccg ctggtggagg acatcgcaaa ggccctgagg gcggacgggc
     3481 atacgggtct gcgctgacgc gcaaacgggc ggacggggta agaacaacag accattatgg
     3541 ccttttcatg aaaaaaaggt acaatatgtt gatgtgtatg gtccggatga caacgagaca
     3601 ggtcaacaag actcttcctc atgtgcccgt gtgcctgatt gcgcgaagcg cagacccgtg
     3661 ggcgagtcca ggtgaaaaat gatgaaacgc agagcattca cagtgcgcag gtcgaaccgg
     3721 ccggtgaaga aggagatcac ggggtccatc ggggatatcg tcaaagagat cactcgggac
     3781 aggaacgtca agaacgaaca gtaccgcgag caatcgctga agatgcacgg cctcatgtgc
     3841 gcgaggtgcg gcagggaatt cgaccacacg aacaggcatc ttctgaccgt gcaccacaga
     3901 gacggcaacc acaacaacaa tccgccggac gggtccaatt gggagaacct gtgcgtctac
     3961 tgccacgacg acgagcacgg caggggtctt ctgggag
//
LOCUS       DAOQTR010000154         3988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25469_length_3988_cov_3.202136, whole genome shotgun sequence.
ACCESSION   DAOQTR010000154 DAOQTR010000000
VERSION     DAOQTR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25469_length_3988_cov_3.202136"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="PKN85_04885"
     CDS             complement(<1..1878)
                     /locus_tag="PKN85_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43772.1"
                     /translation="MKGESGRDREPFEKISDLKRKIKELEESEAGRRRSEEALKEAGD
                     SLRACLENAPDGVYMMDMEGNFLYGNRKSEEIIGYSRDELIGRNFLEMNLLSEASFLK
                     ADELFQASMEGKPTGPDEIELVSRDGRPVPVEVNTSVAQYMGQRVVIGFVRDITSRKR
                     AAQEILLEKEKLNTLLDNAPFGMLLVDREDRIVYMNTHFTDLFGYDMSDVPDVGTWYS
                     KAYPDARYRDTVTTAVKEDLEAANPGARKPRLFTVTCKDGTEKVVNLIMSVLASGDYL
                     MSCEDITEMRRLESQLRHAQKMESIGTLAGGIAHDFNNILSALMGYASLMQIKMEKDN
                     PLRPYLDQILSASRKAADLTQSLLAFSRKQPVTLLPLDINSTIGSTKKLLKRLLTEDI
                     DLSTSLTRDDTVVMADRSQVDQILFNLVTNARDAMPRGGTLTIKTDIAEIDERFTRTH
                     GFGSPGQYVVITILDTGEGMDEATRRKIFDPFFTTKETGKGTGLGLATVYGIVKQHNG
                     YITVQSEPGRGSAFRIYLPAVRAKTDHEQEAAIPNDPGTGTILIAEDNQEVRRFIREA
                     LREYGYTTIEAVDGEDAVSKFREHGDIDLVIVDSVMPKKNGREVYEEVHRMDPRARVL
                     FM"
     gene            2168..2503
                     /locus_tag="PKN85_04890"
     CDS             2168..2503
                     /locus_tag="PKN85_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43773.1"
                     /translation="MGRSLVISVVLACFLAAGCGGLFPTKIGDIVRDPRKYEGKEVTV
                     SGEVKEVLALVVFKSFVLKDDTGEITVITSRILPAVGQKLKVTGTVKEAFALGTGKLL
                     VIQERGDPR"
     gene            2751..>3988
                     /gene="ablA"
                     /locus_tag="PKN85_04895"
     CDS             2751..>3988
                     /gene="ablA"
                     /locus_tag="PKN85_04895"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNT43774.1"
                     /translation="MHLTKTRNQTAPTIKDMAQPLLWNDWRWQIRHCVRDLTTLENLL
                     GIEFDDARREQYEHTISRFPMSITPYYLSLVDVENLDHDPIFKQSFPSVLEMNISAHD
                     MTDPLHEDEDSPVPGITHRYPDRVLFLVSNTCSMYCRHCTRKRRVGDRDSIPDRSQVM
                     AGLDYIRETRQVRDVLLSGGDPFLLSDDYLDWILTELDDIDHVEVVRIGTRTPVVLPY
                     RITDNLVNVLKKHHPLWINTHFNHPRELTSSSRRALAKLADAGIPLGNQSVLLAGIND
                     CPRIMKSLVHKLVANRVRPYYLYQCDLSEGLSHFRTPVGKGIEIVESLIGHTSGFCVP
                     TYVIDAPAGGGKIPVMPHYLISWSTNKVVLRNYEGVITTYKEPDSYEPVFCDRNCAQC
                     DLQLELDDSVEHTSIGIEGLL"
BASE COUNT          809 a         1196 c         1105 g          878 t
ORIGIN      
        1 cataaagagc acccgcgcac gggggtccat tcggtgtacc tcttcgtaga cctcccgtcc
       61 gttctttttc ggcatcacgg aatcgacgat tacgaggtca atatccccat gttctctgaa
      121 cttgctgacc gcgtcctcgc cgtccacggc ctcgatggta gtatacccgt attcgcggag
      181 ggcctcccgt atgaaccgtc tgacctcctg gttgtcttcg gcgatgagga tcgtgccggt
      241 ccccggatcg ttgggaatag ccgcttcctg ttcgtggtcc gtcttggccc gcaccgcggg
      301 aagatagatg cggaaggcgc tgccgcggcc cggctcgctt tgcacggtga tatacccgtt
      361 gtgctgtttc acgataccgt acacggtggc aaggccgaga cccgtgcctt tgcccgtctc
      421 cttggtggta aagaaggggt cgaagatctt ccgccgggtc gcctcatcca tgccctcccc
      481 tgtatccaaa atggttatca ccacgtattg tccgggcgag ccaaacccat gggttctcgt
      541 aaacctctcg tcgatctccg cgatatccgt ctttatggtg agcgtacccc cgcgcggcat
      601 ggcatccctc gcgttggtga cgagattgaa gaggatctgg tccacctgcg acctgtccgc
      661 cattaccacc gtatcatccc gggtaaggga cgtgctcaga tcgatgtcct cggtcagaag
      721 cctcttgagg agcttctttg tcgatcctat cgtactgtta atgtccaggg gaagaagagt
      781 cacgggttgc ttcctgctga aggccagaag actctgggta aggtcggccg ccttccggga
      841 ggccgacagg atctggtcca ggtatggccg aagaggattg tccttttcca tctttatctg
      901 catgagagag gcgtatccca tcagggccga gaggatattg ttaaagtcgt gtgcgatgcc
      961 ccccgccagg gtgcctatcg attccatctt ctgggcgtgg cggagctggg attcgagcct
     1021 tctcatctcc gttatgtctt cgcagctcat caggtagtcg ccggaggcca gcacggacat
     1081 gataaggttt acgaccttct ccgtgccgtc cttgcacgtg acggtgaaca gccgcggctt
     1141 cctcgccccg gggtttgccg cttcaaggtc ttccttcacc gccgtggtaa ccgtgtccct
     1201 gtacctcgca tcggggtagg cctttgagta ccatgtcccc acatcgggga catctgacat
     1261 gtcatatccg aagaggtccg tgaaatgggt gttcatgtag acaatgcggt cctccctgtc
     1321 gacgagaagc ataccgaagg gggcgttgtc caggagggta ttgagcttct ccttttccag
     1381 aaggatctct tgagcggccc gttttcggct ggtgatgtcg cgcacgaaac cgatgacgac
     1441 cctctggccc atgtattgag ccacgctcgt attgacctcc acaggaacag gacgcccgtc
     1501 cctgctgacg agttcgatct cgtcgggacc ggtgggcttg ccttccatgc tggcctggaa
     1561 caactcgtcc gccttgagga agctcgcctc cgacagcagg ttcatctcaa ggaagttcct
     1621 gccgatcagc tcatccctgc tgtagccgat gatctcctcg ctcttccggt tgccgtacag
     1681 gaagttgccc tccatgtcca tcatgtagac gccgtccggg gcattctcca ggcaggccct
     1741 caacgagtcc ccggcctctt tcagggcctc ttccgagcgc cgtcgtccag cttcggactc
     1801 ctccagttcc ttgatcttcc gcttgagatc ggagatcttt tcgaacggtt cccgatctct
     1861 tcccgactcg cctttcatct ggtctcctta cggtcataaa gagtgcctgt gtgtcagaac
     1921 atatgaaata ctatagtgat aaaatgagaa aatgcaatcc tcgaccacga gaaccatcaa
     1981 cgcgatagca ctggttgtcc tttgattgtc ttatcgcccg atgccgggac gaggttaaga
     2041 gaaccatcgg atcacgtggt cgtccatcca gcattgccgg gcccgcggcg tcggccggga
     2101 cgcgttgccg gcatcgctgt gttatactca aacagagaga tccgtatttt cgccggggag
     2161 gtaaaccatg ggaagaagcc tggtcatttc cgttgtgttg gcctgttttc ttgccgccgg
     2221 gtgcggcggc ctctttccga ccaagatcgg ggacatcgtc agggatcccc ggaaatatga
     2281 gggaaaagag gtgaccgtgt cgggtgaggt gaaggaggtc cttgccctcg ttgtcttcaa
     2341 gtccttcgtc ctcaaggacg acaccggcga gatcacggtg atcacatcac ggatcctgcc
     2401 ggcagtcggc cagaaattga aagtcacggg aacggtcaag gaggccttcg ccttgggcac
     2461 cgggaaactc cttgtcatac aggaaagagg cgatccccgg tgaaagcggg cacgctgggc
     2521 cccctgccga cgatgggatc ccgggtcaac aggccgcgca cgccggctgt ctagtttctt
     2581 ctgacaaaag taacctgttt gacagaagca catgggtctt ccggggtata tataaatggg
     2641 tgtcaaaaga cattcggcaa cccgacacaa ggagaaggcc ctttttccgg ggccggaaag
     2701 cacgtggagg gcacctcgag aagcggtgtt ctgcgctaaa ggaggaaacg atgcacttga
     2761 caaaaacccg gaaccagact gctcccacga tcaaagatat ggcacagccc cttctctgga
     2821 atgactggag atggcagatc agacattgtg tccgcgacct cacgaccctg gagaacctcc
     2881 ttggaattga attcgatgac gcgcgacgtg agcagtacga gcacaccatc agccgttttc
     2941 ccatgtccat aacgccgtac tacctttcgc ttgttgacgt ggagaacctg gaccacgacc
     3001 cgatcttcaa gcaaagcttt ccctccgtgc tggagatgaa tatcagcgcc cacgacatga
     3061 ccgatccgct ccacgaggac gaagacagcc ccgtccccgg catcactcac cgctatccgg
     3121 acagggtgct gttcctcgtc agcaacacct gctcgatgta ttgtcgccac tgtacccgga
     3181 agcggcgcgt gggcgaccgt gacagcatcc ccgaccgttc ccaggtaatg gccgggctcg
     3241 actacatacg ggagacccgg caggtccggg acgtcctgct gagcggaggc gatccgttcc
     3301 tgctgtccga cgactacctc gactggatcc tcaccgaact ggatgacatc gaccacgtgg
     3361 aggtggtccg catcggaacg cgcacgcccg tcgtgcttcc ctaccggata accgacaatc
     3421 tggtgaacgt cctcaagaaa catcaccccc tctggatcaa cactcacttc aaccatcccc
     3481 gggagctgac ctcctcctcc cgaagggccc tggccaagct cgccgacgcg ggcatacccc
     3541 ttggcaacca gtccgtgctt ctggcgggca tcaacgactg tccacgcatc atgaagtcgc
     3601 tggtccataa actggtggcc aaccgggtca ggccatacta tctctaccag tgtgacctgt
     3661 ccgagggttt gagccatttt cgcacacccg tgggcaaagg catagagatc gtcgaaagtc
     3721 tcatcgggca caccagcggg ttctgcgtgc cgacctacgt gatcgatgca ccggcaggcg
     3781 gagggaagat ccccgtgatg ccccactacc tgatctcctg gtcaacgaac aaggtggtgc
     3841 tgagaaacta cgaaggtgtt ataacgacat acaaggaacc cgattcatac gaacccgttt
     3901 tttgcgaccg gaattgtgcc caatgcgatc ttcagctcga actcgacgac agcgttgaac
     3961 atacgagcat aggtatcgag gggcttct
//
LOCUS       DAOQTR010000155         3974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25594_length_3974_cov_3.092881, whole genome shotgun sequence.
ACCESSION   DAOQTR010000155 DAOQTR010000000
VERSION     DAOQTR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25594_length_3974_cov_3.092881"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKN85_04900"
     CDS             <1..614
                     /locus_tag="PKN85_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43775.1"
                     /translation="LSLGNIDIMFGMVPKFNVRDVIDGSRQMSEVILEGPCGIRIIPA
                     TSGVSEFSHLDEEGRMILMSSLSTLPKHDFLIVDTSAGISSNVVYFNAMSHDIFVVVT
                     PDPASITDSYAMVKVLSTKTGRKDFNIIINMVREEKEALELFRKVVGVTDRFLDVYLN
                     YFGYIPLDQHVSISTRKQRLWAEHFPDAVATRALAKICDRLVS"
     gene            611..1354
                     /locus_tag="PKN85_04905"
     CDS             611..1354
                     /locus_tag="PKN85_04905"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNT43776.1"
                     /translation="MMTWKKGYAKTFRDERERTIDEFIPIIKHLAYKVSRRYEDDEII
                     EDLISAGIVGLLEAMDKFDATKGAKLNTFAYLRIRGAMIDELRSRDWFPRSARSKSKK
                     INEVVRKLENKLGRLPKEEEVAEALGMDLDDYFKMLKNYGNLSVLSIEDLHELLGEDR
                     DRIMRYVTEESDDPEKCAEVLELERIMAGEFDKLPERQRYVLSLYYHEDLNMKEIARV
                     LSITEARVCQIHSQAILNLRVSMKKYLRN"
     gene            1393..2292
                     /locus_tag="PKN85_04910"
     CDS             1393..2292
                     /locus_tag="PKN85_04910"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT43777.1"
                     /translation="MEREEILKRLRKIDVLPTFPAVMAEVISVIEDPMSSATDLARTM
                     DPSMAGEVLRCANTAYFGTRNFRKITSIEHAIAIVGLEQLSQMILHMPFLSMMGGEGG
                     LDRERFVRHSMICGIVSKVISRTLHAGEEHAVYIGGLMHDIGAIIIYRYFNEEWKVMS
                     ALVRNEGRTWTQAEEDVLSCDHGMIGACLLTMWNIPQAVTDGVMHHHAPHRAAENVWN
                     AKLIGVGNRFAKNVDLSDNFASLDEFINNNKDFIPLVEETGLDVTLSQELDLFEGVYT
                     LMKNARGLLSCAGDVEDDQGTGS"
     gene            2270..3301
                     /locus_tag="PKN85_04915"
     CDS             2270..3301
                     /locus_tag="PKN85_04915"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNT43778.1"
                     /translation="MIKVLVVDDSLVMRKTISRMLSKDKAIEVVGTAVDGRDALEKVE
                     SLKPDVITMDVEMPVMNGIEALKKIMEKDPVPVIIMSALTREGAEITMEALHLGACDF
                     VTKDFTNLGNSFSSKEAEVIAKVKNVAKNKVRFLLRKLDIKPRPAAVISPDQKVRHEV
                     LAIGASTGGPPALQHILTRLPRAFPVPIVIAQHMPKIFTQSFSQRLDAVSQLQVKEAE
                     DGETLRAGIAYIAPGNTHMAVRRKGRSVFVQFIEGTQYIYRPSVDLLMSTTAETFERQ
                     SFGVILTGMGNDGVQGMKEMKAKGGYIVAQDEETCVVFGMPRAVISANLADAVLPIDK
                     ISEEIMRVL"
     gene            3298..>3974
                     /locus_tag="PKN85_04920"
     CDS             3298..>3974
                     /locus_tag="PKN85_04920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT43779.1"
                     /translation="MRTQEREDVRMQGLGGEELEHFITLLRDGDNFEKGKAIEALVES
                     PGKEVVERIVPLLQEKNTPVRMAVLDVLKRIGSVHLDGVIGMLEDENEDIRVYACEVL
                     SFLRDPRSIPFIVKKSRDDVDNVRNAACMALGEFDDDRAVMALLDALKDEEWIAFSAI
                     LSLGRAKSPKAVPRLLQFFRESGEELSDAACEVLVEYGDDAVLDELFDVLKGWDREKR
                     SAYLKIS"
BASE COUNT          960 a         1070 c         1200 g          744 t
ORIGIN      
        1 acctttccct ggggaacatc gacatcatgt tcggcatggt cccgaagttc aacgtccgcg
       61 acgtcatcga cggctcccgc cagatgagcg aggtcatcct cgaggggccc tgcggcataa
      121 ggatcatacc ggccacgtcg ggggtaagcg agttctctca ccttgatgag gagggcagga
      181 tgatccttat gagctcgctc agcaccctgc cgaagcacga ttttctcatt gtcgacacct
      241 ccgcggggat ctcctccaac gtggtctatt tcaacgcgat gagccacgac atcttcgtcg
      301 tcgtcacgcc ggacccggcg agcatcaccg actcctacgc gatggtgaag gtcctgagca
      361 cgaagacggg caggaaagac ttcaatatca tcatcaatat ggtgcgggaa gagaaggaag
      421 ccctcgagct gttcaggaag gtggtcggcg tcaccgacag gtttctcgat gtatatctca
      481 attatttcgg ctacatacct ctcgatcaac atgtgagcat ctcaacacga aagcagaggc
      541 tgtgggcgga gcattttccc gatgctgtcg ccacgcgggc gcttgcaaaa atctgtgaca
      601 ggttggtgtc atgatgactt ggaaaaaagg atacgcaaag acattcagag acgaacgtga
      661 aaggaccatc gacgaattca tccccatcat caagcacctg gcttacaaag tgtcccggcg
      721 atacgaggat gacgagatca tagaagacct catctccgcg gggatagtgg ggctcctgga
      781 ggcgatggac aagttcgacg ccacgaaggg cgccaagctc aacacattcg cctatctcag
      841 gatacggggc gccatgatcg acgagctgcg ttcccgtgac tggttcccga gaagcgcccg
      901 gtcgaaatcc aagaagataa acgaggtcgt caggaaactg gagaacaagc tcggaaggct
      961 gccgaaggaa gaagaggtgg ccgaggcgct gggaatggac ctcgacgact atttcaagat
     1021 gctcaagaac tacgggaacc tttccgttct cagcatagaa gacctccacg aactcctggg
     1081 cgaggaccgc gacaggataa tgcgctacgt aacggaagag agcgacgacc ccgagaagtg
     1141 cgcggaggtc ctggaactgg agcgcataat ggccggcgag ttcgacaagc tgccggagag
     1201 gcagaggtat gtcctgagcc tctactacca cgaggacctg aacatgaagg agatagcccg
     1261 cgtccttagc atcaccgaag cccgtgtctg ccagatccat tcgcaggcga tcctgaacct
     1321 gagggtatcc atgaagaaat atctgcgcaa ttaaaggttt caagcgggct tccgataata
     1381 tgtctgacag ttatggaacg cgaagagatc ctgaaaagac tcagaaagat agacgtgttg
     1441 cccacatttc cggcggtcat ggcggaggtc ataagcgtca tagaggaccc gatgagctcc
     1501 gccacggacc ttgcaaggac gatggacccc tccatggcgg gcgaagtcct gcgttgtgcc
     1561 aataccgctt atttcgggac tcgcaacttc cgcaagatca cgtccattga gcacgccata
     1621 gccatagtcg ggttggagca gctttcgcag atgatcctcc acatgccctt tctgtccatg
     1681 atgggcggcg agggagggct cgacagggag aggttcgtac gccattccat gatatgcggc
     1741 atagtctcga aggtcataag ccgcaccctc catgcgggag aggagcacgc cgtctacata
     1801 ggcggtctca tgcacgacat cggggcgatc atcatatacc gctatttcaa cgaggaatgg
     1861 aaggtgatga gcgctctcgt gaggaacgag ggcaggacat ggacgcaggc ggaggaggac
     1921 gttctttcct gcgaccacgg gatgataggg gcatgccttc tgaccatgtg gaacattccc
     1981 caggcggtga ccgacggggt catgcatcac cacgctcccc atagggccgc cgagaatgtg
     2041 tggaacgcga aactcatcgg tgtgggaaat aggtttgcaa aaaacgtcga tctgagcgat
     2101 aatttcgcca gtctcgatga atttattaat aataacaaag acttcattcc gctcgtcgag
     2161 gagacgggac ttgacgtgac gctttcacag gagctggacc tcttcgaggg ggtatacacc
     2221 ctcatgaaga acgcgagggg tcttctatcc tgcgcaggag acgtagagga tgatcaaggt
     2281 actggtagtt gacgattcac tggtgatgcg caagacgata tcgaggatgt tgtcgaaaga
     2341 caaggccatc gaggtcgtgg gcaccgccgt tgacggcagg gacgcccttg agaaggtgga
     2401 gtccctgaaa cccgacgtta taaccatgga cgtggagatg cccgtcatga acggcatcga
     2461 ggccctgaag aagatcatgg agaaggaccc ggtgccggtg atcatcatga gtgccctgac
     2521 cagggaaggg gccgagatca ccatggaagc cctgcatctc ggtgcctgtg atttcgtgac
     2581 caaggacttc acgaatctgg gcaactcctt ctcgtcgaag gaggcggaag tgatcgcgaa
     2641 ggtgaagaac gtggcaaaga acaaggtcag gttcctcctt cgcaagctgg atatcaagcc
     2701 cagacccgcc gccgtgatca gcccggacca gaaggtccgt cacgaggtcc ttgccatagg
     2761 cgcctccacg ggcgggccgc cggccctgca gcacatcctg acgaggctcc cccgcgcctt
     2821 cccggtaccc atcgtcatcg cccagcacat gcccaagatc ttcacccagt ccttttccca
     2881 gagactcgac gccgtgtcgc agctgcaggt gaaggaagcc gaggacgggg agaccctgcg
     2941 ggcgggcatc gcctacatcg cccccgggaa cacacacatg gcagtcagga gaaagggcag
     3001 gagcgtcttt gtccagttca tcgaggggac acagtacatc taccggcctt ccgtcgacct
     3061 tctcatgagc accacggcgg agaccttcga gagacagtct ttcggtgtca tcctgaccgg
     3121 tatgggcaat gacggggttc agggcatgaa ggagatgaag gccaaaggcg gatacattgt
     3181 ggcccaggac gaagagacat gcgtcgtctt cggcatgccc agggcggtga tctcggccaa
     3241 cctggccgac gccgtgcttc ccatcgacaa aatatcggaa gagatcatga gggtgttatg
     3301 agaactcagg aaagagagga tgttcggatg caggggcttg gcggtgaaga actggaacac
     3361 ttcatcactc ttctcaggga cggcgacaat ttcgagaagg gaaaggcgat agaggcgctc
     3421 gtcgagtccc cgggaaagga agttgtggag cgcatagtcc cccttcttca ggagaagaac
     3481 acgcctgtca gaatggccgt tctcgatgtc ctgaagcgga tagggagcgt tcatctcgac
     3541 ggcgtcatcg ggatgctcga agacgagaac gaggacatac gggtctatgc ctgcgaggtc
     3601 ctctccttcc tcagggaccc gcgctccata ccattcatcg tgaagaagtc ccgcgatgac
     3661 gtggacaacg tgaggaacgc ggcctgcatg gcgctcggcg agttcgatga cgaccgggcc
     3721 gtcatggccc tccttgacgc cctgaaggac gaggaatgga tagccttttc cgccatcctg
     3781 agcctgggaa gggcgaagag cccgaaggcc gtcccccggc tcctccagtt cttcagggaa
     3841 agcggcgagg aactctcgga cgcggcctgt gaagtcctcg tggaatacgg tgatgatgcc
     3901 gtcctcgacg agctgttcga tgtgctcaag ggatgggaca gggagaaaag gagcgcctac
     3961 ctcaagatca gcga
//
LOCUS       DAOQTR010000156         3962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25729_length_3962_cov_3.765293, whole genome shotgun sequence.
ACCESSION   DAOQTR010000156 DAOQTR010000000
VERSION     DAOQTR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25729_length_3962_cov_3.765293"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /gene="had"
                     /locus_tag="PKN85_04925"
     CDS             <1..921
                     /gene="had"
                     /locus_tag="PKN85_04925"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HNT43780.1"
                     /translation="FDGVPTVSKPPLALGHEIAGTVIAGDPAWIGKEVIIPAVMPCRQ
                     CILCKTGRGNRCLAQKMPGNSIGVYGGFASHIVVPTIDLCLVQDKKGYKLEQLAVVAD
                     AVTTPYQACKRADLQPGDNVIIIGATGGVGVYMAQTAKALGAKTVIGIARNQEKLQRA
                     LGYGCDYVISTLDKTNKDVVGEWRNICKANGLANTGWKIFEVTGSKAGQDLALDLLSF
                     VGKLILVGFGMAKSEYMFSKLMAFDAEVIGTWGCLPEYYPIVLDMVLSKKIQIDPFVE
                     VRPMSTIAATFEEIHKAGSPAKRVVLTPDF"
     gene            953..2098
                     /gene="oah"
                     /locus_tag="PKN85_04930"
     CDS             953..2098
                     /gene="oah"
                     /locus_tag="PKN85_04930"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HNT43781.1"
                     /translation="MGLEWMPREHEKKDHFRFTDQHWGTEAPCVVYERKPLKDNKGNV
                     VPGLYVAWIRLNNPSQFNSYTTEMVKGVIAGFENSSVDREVVGVVFTGTGPFAFCTGG
                     NTKEYSEYYSRRPEEYGAYMELFNNMVDSILMCKKPVISRVNGMRVAGGQEIGTACDI
                     TLTSDLAIFGQAGPRHGSAPVGGASDFLPSYMSIEDAMWSCISCEMWSSYKMWWKGLL
                     TKAFPVLKDDKGNWVRNPQVITDKFIENGEIVYGENKAGQEFKDARAWVGEKQKAGDV
                     DFSLMDAEIDRIMWTYANLFPGCLMKSIDGIRQKKKFFWDTMKNEHRHWLAANMGIEA
                     FLGFGAFNTKKITGMDTIDFIKYRKGVADGVLMDDTFMAEVLGKPQK"
     gene            2163..2933
                     /locus_tag="PKN85_04935"
     CDS             2163..2933
                     /locus_tag="PKN85_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT43782.1"
                     /translation="MAFTHITFQKKNKVATITLNEPPSNWLTILMMREIVEAIMDVKK
                     DPTIQLLVFDHAGEKAFCDGVDVADHTPDKVLDMIEVFHRMFRLMEELDCTTVALVNG
                     RSLGGGCELMAFCDIVIASEKAKIGQPEIAVGVFPPIAAAWFPKIIGLKNTYELLLTG
                     KIIGAKEAQAMGLVNAVLPVENFKAEADKFMADFLKQSRPVAMWTKRAIKAGLNVNFL
                     EALRASEICYLDGCMKTKDAVEGISAFMDKRKPVWKDE"
     gene            2943..>3962
                     /locus_tag="PKN85_04940"
     CDS             2943..>3962
                     /locus_tag="PKN85_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNT43783.1"
                     /translation="MKYAPKEEIEARITKLKTLMEKASLDGAFFHYKIDYYYLSGTMQ
                     DSLLFVPLDSDPVLFVKREISRARRESPLRNIMSYRSIKDIPQHLKPMRRLGMQLDVI
                     PYNDAIRFKDLFSDVEFVDCSPLIKELRKQKSPFEIGLMEKAAEIGRKVYAKVPEFLR
                     EGATEIEVGGQMEAYAKSLGHEGLLRVRSLNYEAYTWHILSGRTGTIVSQSDSPMGGL
                     GLSPAFPVGASFKRIRRNEPILVDFGICYHGYQVDQTRMYAVGSMPDMFVRGYDACRE
                     IHDRVLDKVVEGYTSKELFEYSKELGDKLGYGDYYLGYKPHKVRFLGHGIGIELAELP
                     YIAATH"
BASE COUNT          946 a         1149 c         1142 g          725 t
ORIGIN      
        1 tttgacggtg ttcccaccgt gagcaaaccg cctctggcgc tgggtcacga gatagccggc
       61 accgttatcg ccggtgatcc cgcatggatc ggcaaagagg tcatcattcc cgcagttatg
      121 ccctgcaggc agtgcatact ctgcaagacc ggcaggggca acaggtgcct ggcccagaag
      181 atgcccggca actcgatagg cgtgtacggc ggcttcgcga gccacatcgt cgttcccacg
      241 atcgacctgt gccttgtcca ggacaagaaa ggttataaac tggaacagct cgccgttgtg
      301 gcggacgcag tgaccacccc gtaccaggcg tgcaagaggg ccgaccttca gcccggtgac
      361 aatgtcatca tcatcggcgc gacgggcggc gtgggcgtgt acatggccca gaccgcgaag
      421 gcgctcgggg ccaagaccgt catcggcatc gccaggaacc aggagaaact ccagagggcc
      481 ctgggctacg gctgcgacta cgttatcagc accctcgaca agacgaacaa ggatgtcgtc
      541 ggcgagtgga ggaacatctg caaagccaac ggcctcgcca acacaggctg gaagatcttt
      601 gaggtcaccg gctccaaggc cggccaggat ctcgccctcg atcttctctc cttcgtgggc
      661 aaactcatcc tcgtcggctt cggcatggcg aagagcgagt acatgttctc caagctcatg
      721 gccttcgacg ccgaagttat cggcacctgg ggctgtctgc ccgagtacta cccgatcgtc
      781 ctcgacatgg tcctcagcaa gaagatacag atcgacccct tcgtcgaagt gcggccgatg
      841 agcaccatcg cggcgacctt cgaggagatc cacaaggcag gttcacctgc gaaacgcgtt
      901 gtcctgacac ctgattttta aggtttcaaa caaaaacttt taggaggaaa gtatgggatt
      961 agaatggatg ccaagagaac atgagaagaa agaccatttc aggttcaccg atcagcactg
     1021 gggaacggaa gccccctgcg tagtgtacga aagaaaaccc ttgaaggaca acaagggcaa
     1081 cgtcgtcccc ggcctgtacg tagcatggat caggctcaac aacccctcac agttcaactc
     1141 ctacacgacc gagatggtca agggcgtcat cgccggtttc gagaactcat ccgttgaccg
     1201 cgaggtcgtc ggcgttgtct tcaccggcac gggccccttc gcattctgca ccggcggcaa
     1261 cacgaaagag tactcagagt actacagcag acggccggaa gaatacggcg cgtacatgga
     1321 actcttcaac aacatggtcg actccatcct catgtgcaag aagccggtca tctcccgcgt
     1381 gaacggcatg agggttgccg gcggccagga gatcggcacc gcctgcgaca tcacccttac
     1441 ctctgacctc gccattttcg gtcaggcagg ccccaggcac ggctccgccc ccgtcggcgg
     1501 cgcctccgac ttcctcccct cctacatgag catcgaagat gccatgtgga gctgcatcag
     1561 ctgcgagatg tggtcctcct acaagatgtg gtggaagggc cttctcacca aggcattccc
     1621 cgtcctgaaa gacgacaagg gcaactgggt acggaacccg caggtcatca ccgacaagtt
     1681 catcgagaac ggcgagatcg tctacggcga gaacaaagcc ggtcaggagt tcaaggatgc
     1741 gcgggcgtgg gtcggcgaaa aacagaaagc cggcgacgtc gacttctccc tcatggatgc
     1801 ggagatcgac aggatcatgt ggacctacgc gaacctcttc ccgggctgcc tcatgaaatc
     1861 catcgacggc atccgccaga aaaagaaatt cttctgggat accatgaaga acgagcatcg
     1921 gcactggctc gccgccaaca tgggcatcga ggccttcctc gggttcggcg cgttcaacac
     1981 caagaagatc accggaatgg acaccatcga tttcatcaag taccgcaagg gcgtcgcgga
     2041 cggcgttctc atggacgata ccttcatggc ggaagttctc ggcaaacctc agaagtaatt
     2101 acgaggcaaa ctctaggggg gagcacatcc cccctatttt ttttgaaaga aggagaacca
     2161 tcatggcttt cacgcacata acatttcaaa agaagaacaa ggtcgcaacg ataaccctca
     2221 acgagccccc ctcgaactgg ctgaccatcc tcatgatgcg ggagatcgtc gaggcgatca
     2281 tggacgtcaa gaaagacccg acgatccagc ttctcgtctt cgatcacgcc ggagaaaagg
     2341 cgttctgcga cggcgtcgac gtggccgacc acaccccgga caaggtcctc gacatgatcg
     2401 aggtcttcca ccggatgttc cggctcatgg aagagttgga ctgcacgacg gtggcgctgg
     2461 tgaacggcag atcgctcggc ggcggatgcg agctcatggc cttctgtgac atcgtcatcg
     2521 cttcagagaa agcgaagatc ggccagcccg agatcgcggt gggcgtgttc cctccgatcg
     2581 cggcggcatg gttcccgaag atcatcggcc tcaagaacac ctatgagctt ctcctgacag
     2641 gcaagatcat cggcgcgaaa gaggcgcagg caatgggtct cgtcaacgct gtccttcccg
     2701 tggagaactt caaggcggaa gcggataagt tcatggccga tttcctcaag cagtcgaggc
     2761 cggtggccat gtggacgaag cgcgccatca aggcgggtct caacgtcaat ttcctcgagg
     2821 ccctgagggc gtccgagata tgctacctcg acggctgcat gaagacgaag gatgccgtcg
     2881 aagggataag cgccttcatg gacaagagaa aaccggtgtg gaaggacgag taaccgggcc
     2941 agatgaaata cgcgccgaaa gaagaaatag aagcacggat cacaaagctg aaaacgttaa
     3001 tggaaaaggc ctctctggat ggggcctttt ttcattacaa aattgactat tactacctct
     3061 cgggcacgat gcaggactcc ctgctcttcg tccccctcga ctccgacccg gtgctcttcg
     3121 tcaagcgcga gatatcgaga gcgcgcaggg agtctcccct ccggaacatt atgtcctatc
     3181 ggtccatcaa ggacatcccg caacacctca agcccatgag gcgactgggc atgcagcttg
     3241 acgtcatacc ttacaacgac gcgatcaggt tcaaggacct cttcagcgac gtggaattcg
     3301 tcgactgctc gccgctcata aaggaactga gaaagcagaa atctcctttt gagataggcc
     3361 tcatggagaa ggccgcagag ataggcagga aggtatatgc gaaggtgccg gagttccttc
     3421 gggagggggc aacggagatc gaggtgggcg gccagatgga ggcctacgcg aagtcgctgg
     3481 gccacgaagg gctcctgagg gtccggtccc ttaactacga ggcctacacc tggcacatcc
     3541 tgagcggacg cacgggcacc atagtgagcc agtccgattc acccatgggc ggcctcggcc
     3601 tgtccccggc atttcccgtc ggcgccagtt tcaaaaggat aaggaggaac gagcccatcc
     3661 tcgttgattt cgggatctgc tatcacggct accaggtaga ccagaccaga atgtatgccg
     3721 tcggttcgat gccggacatg ttcgtcaggg gctacgatgc ctgcagggag atccatgacc
     3781 gcgtgctcga caaggtcgtc gaaggataca cgagcaagga gcttttcgag tactccaaag
     3841 aactggggga caagctggga tacggtgact actatctcgg gtacaagccg cacaaggtgc
     3901 ggttcctggg tcatgggatc ggcatcgaac tggcggagct tccttacata gccgccaccc
     3961 at
//
LOCUS       DAOQTR010000157         3961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25735_length_3961_cov_4.048131, whole genome shotgun sequence.
ACCESSION   DAOQTR010000157 DAOQTR010000000
VERSION     DAOQTR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25735_length_3961_cov_4.048131"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PKN85_04945"
     CDS             complement(<1..609)
                     /locus_tag="PKN85_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT43784.1"
                     /translation="MEIELSVRKKLFTAAGEFPFTADLAIASGEMVSFFGPSGVGKTT
                     MLRIIAGLTDADEGFVRVGGQVWYDSTGGTNLPPQRRKVGFVFQEYALFPNMSVRENL
                     RFAQPKTDKRHIDELLDIFELSALQHRKPGFLSGGQNQRVALARALARKPDLLLLDEP
                     LSALDHEMRNTLQDEILEVHRLWGITTILVSHDLPEIFKLCNR"
     gene            complement(599..1021)
                     /locus_tag="PKN85_04950"
     CDS             complement(599..1021)
                     /locus_tag="PKN85_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNT43785.1"
                     /translation="MNTLRGKISEMTTSGNMTLVGVDVDGTRMTAIVIGTPGKASYLR
                     MAEHVELLFNESAVSIGKMIEGQMSLNNQLDCTIERLVTGEILTQVILSFRGEHLTSL
                     TTTMSARRLDLKAGDRVTAFIKTNEVMLREPDEAGNGN"
     gene            1332..1922
                     /locus_tag="PKN85_04955"
     CDS             1332..1922
                     /locus_tag="PKN85_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43786.1"
                     /translation="MRYHQLIAWIRDEIASTKVVLSEEVVGYEETLEPNPEMYWSEST
                     DLPTTRDRQHVIIKTPIVEVVERTVPDRERRSHAAEALAAFVLSDERYLYLAEELMRR
                     DPLYLPHWERLLLHGRGIRTRKAALRHLGRAPWQIFIRAVGAVAAVFGSLCAVCALIC
                     FYAAIVYCIWPDSKVGVWMASVVKRILAEATRMIYD"
     gene            1932..>3961
                     /locus_tag="PKN85_04960"
     CDS             1932..>3961
                     /locus_tag="PKN85_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43787.1"
                     /translation="MKELKVSDKAGKIRRASDDVVREALADLVPGGRTTHRIGGHTDW
                     YHGAAVSPDGSRLITANRDGTARLWDIASGQCLMVFRGHTGEVVRAAFMNHTDRAVTT
                     DDDGTLRLWDCATGACILVLKGHHGRVGGLAVSPDDRYIASSDSFWALRVWSLPEGTC
                     EHSAAGINNVMSILVTRDGNTMLTLDSQHDILRWDFPGLGDPSLVANGDYWDIALSGY
                     GEFVAAGRDGLSCFDVRTGVLKRLYSLEPHKPYKVTTASDETRFVACCSPRETVLVVD
                     RKTGAVEKQFAGPRLVTDMSLSPDGKTLYTVGFDRTCNIWDIGTGECLQAIGSTRSVH
                     HLSLSGNGMYLAACDKGDTVKLWSLTDGSQRAKFPGATSVAFLESARLVVTGHDDGRV
                     RFWDIDSGKEKPGTVLQAYSSAVACVLPLESGAFFATFARHGVPKLWNTEKRNCLRIR
                     NRAGNIDIDTVAIDEDTQRVAMRRQGKNHILVCDLRSGDILATLTDHAYKYGNLSVII
                     TGIRFLPRGRGLVSCGRDGLIILWDLSTFKAARSIHVGNSILSMAVENDGRHVVIGTY
                     AGGVERWDLESGQREICRPPEGSPVAGLALSPDESRLFVSFNDGTVRIIDRQSTALVC
                     SLWNVDDGFFWFTPPDEHGFCSYGVEISTNRSAAQAAKIVIAELNPQMPRTLG"
BASE COUNT          766 a         1222 c         1181 g          792 t
ORIGIN      
        1 ccggttgcac aatttgaata tctccggcag gtcgtggctg acaaggatcg tcgttatccc
       61 ccacagcctg tggacctcga ggatctcgtc ctgaagggtg ttcctcatct catggtccag
      121 cgcggaaagg ggctcgtcga ggagcagcag gtccggcttc cgcgccaggg ccctcgccag
      181 ggccaccctc tgattctgtc cgccggaaag aaagcccggc ttgcggtgct gcagagcgga
      241 cagctcgaag atgtcgagca gctcgtcgat atgcctcttg tccgtcttcg gctgcgcgaa
      301 gcgcaggttc tcgcggacgc tcatgttcgg gaaaagagca tactcctgga acacgaaccc
      361 gaccttgcgc ctctggggcg gcaggttcgt tccgcccgtc gagtcgtacc acacctgacc
      421 gccgaccctc acgaaccctt cgtcggcgtc ggtcaggccc gcgatgatgc gcagcatcgt
      481 cgtcttgccc acgcccgagg ggccgaagaa ggacaccatc tcaccggagg cgatggcgag
      541 gtcggccgtg aaagggaact cccctgcggc cgtgaacagc ttcttcctca ccgacagttc
      601 aatttccatt ccccgcctcg tccggctccc tcagcatcac ttcgttcgtc ttgatgaagg
      661 ccgtcacgcg gtcgcccgcc ttaaggtcca gcctcctcgc cgacatcgtc gtggtcaggg
      721 aggtcaggtg ttcacccctg aaggacagta tcacctgcgt cagtatctcc cccgtcacaa
      781 gcctctcgat ggtgcaatcc agctgattgt tgaggctcat ctgcccctcg atcatctttc
      841 ctatcgacac cgccgactca ttgaacagga gctcgacatg ctcagccata cgcaggtacg
      901 aggcctttcc cggcgtcccg atcacgatcg ccgtcatccg ggtcccgtcc acatcgacgc
      961 ccaccagcgt catgttcccc gatgtcgtca tctcagatat cttcccgcgc aaggtgttca
     1021 tgaccctcaa ttcgttcgac gcttcactga aacactcgtt atatatatac gcacataccg
     1081 cctacgaaag tcaacacaac ctcaaacgcg gcgggccgac tgcggaaaga cgttatctcg
     1141 cccgcttcgc tccccctcac tgttcgctct ttgtgcaaaa tcacccttct cttttgccat
     1201 tgcctgcacg gtgtattgac tgatatccca tacgtgatgc gatgcagcta tttggagtga
     1261 ggtccttggg acgggagatc gggaagttgg ggagcggatc gaaggatgcg caacaagagg
     1321 agcggtcgta catgagatac catcagctca tcgcctggat acgggacgag atcgcgtcca
     1381 caaaggtggt tctcagcgag gaagtggtcg gctatgaaga gaccctggag cccaaccccg
     1441 agatgtactg gtcggagagc accgatcttc cgacgacgcg ggacaggcag catgtcatta
     1501 tcaagacgcc gatcgtggag gtcgtggagc ggaccgtacc ggacagggaa cgtagatccc
     1561 acgctgccga agccctcgcg gcctttgtcc tctccgacga gcggtatctg tacctggcgg
     1621 aggaactgat gaggcgggac cccttgtatc tgccgcactg ggagcgcctt ttgctgcacg
     1681 gcagaggcat caggacgaga aaagccgccc ttcgtcacct tggcagggcc ccgtggcaga
     1741 tctttataag ggcagtcggt gcggtggcgg ccgtcttcgg gtccctctgc gctgtatgcg
     1801 ccctgatctg tttctatgcc gccatcgttt attgcatctg gccggactcg aaagtggggg
     1861 tctggatggc ctcggtggta aagagaatcc tcgcggaagc gacaagaatg atatacgatt
     1921 agaggaaggc catgaaggaa ctgaaggttt cggacaaggc ggggaagata cggcgggcat
     1981 ccgatgacgt cgtgcgggag gcgctggcgg acctcgtccc cgggggaagg accacccatc
     2041 gcataggggg acatacggac tggtatcacg gagcggcggt ctcccccgac gggtcgcggc
     2101 tgatcactgc caaccgcgac ggaacggccc gcctctggga cattgccagc ggccaatgcc
     2161 tcatggtgtt tcgggggcac accggcgagg tggtgcgggc tgcttttatg aaccacacgg
     2221 accgggccgt cacaaccgac gatgacggca ccctgcgcct ttgggactgc gcgacgggcg
     2281 cctgcatcct cgtcctgaaa ggtcatcatg gacgtgtcgg cggcctggcg gtgagccccg
     2341 atgacaggta tatcgcaagc agtgacagct tctgggcact cagggtatgg tcccttcccg
     2401 agggtacttg cgaacattcc gcggcaggga tcaacaatgt catgagtatc cttgtgaccc
     2461 gggacggcaa cacgatgctc acgctggact cacagcacga tatccttcga tgggacttcc
     2521 ccggccttgg tgatccctct cttgttgcga acggtgatta ctgggacatc gctctttcgg
     2581 ggtatggaga gtttgtggcc gccggaaggg acggcctttc ctgcttcgat gttcgcaccg
     2641 gcgtcctgaa gaggctctat tccctggaac cgcacaaacc ctacaaggtg accaccgctt
     2701 ccgacgaaac acggttcgtg gcctgctgca gccccaggga gactgtcctc gtggtggacc
     2761 gcaaaacggg cgctgttgag aaacagtttg ccggtccgag gttggtgacc gacatgagcc
     2821 tctcgccgga tggaaaaacc ctctatacgg tgggctttga ccggacatgc aacatctggg
     2881 atatcggcac gggcgagtgc ctgcaagcca tcggttccac ccggagcgtg caccatctct
     2941 ccctttccgg caacggcatg tatctcgccg cctgtgacaa aggcgacacg gtcaagctct
     3001 ggagcctcac ggatggttcc cagcgggcga agttcccggg ggcaacgtcc gtcgcctttc
     3061 tcgaaagcgc ccgtcttgtt gtcaccgggc atgatgacgg ccgggttcga ttctgggaca
     3121 tcgatagcgg caaagagaag cccgggacgg tcttgcaggc ctactcatcc gcggtcgcct
     3181 gcgtcctgcc gctggaatca ggcgcctttt tcgcaacctt cgcccggcac ggagtgccga
     3241 agctctggaa cacggaaaaa cgcaattgtc tcaggatccg taacagggcc ggaaatatcg
     3301 acatcgacac cgtcgcgata gatgaagaca cacaacgcgt ggcgatgcgt cgccagggga
     3361 agaaccatat acttgtctgc gatcttcggt ccggcgacat tcttgccacg ctgacggacc
     3421 acgcatacaa gtacggcaac ctctccgtca tcatcacggg cattcgtttt ctcccccggg
     3481 gaaggggtct tgtttcctgc ggccgtgacg gccttatcat cctctgggac ctttccacgt
     3541 tcaaggccgc gcgttctatc catgtaggta attcgatcct ctccatggcc gtcgagaacg
     3601 acgggcgtca tgtggtcatc ggcacctatg cgggaggggt ggaacgatgg gacctcgaaa
     3661 gcgggcaacg cgagatctgc cggcctcctg aagggagtcc ggtggcgggg ctcgcgctct
     3721 ctcccgacga aagccggctc ttcgtgtcct tcaacgacgg gaccgtgagg atcatagaca
     3781 gacagagcac ggccctcgtc tgctccctct ggaacgtgga tgacggcttc ttctggttca
     3841 caccgcccga cgagcacggc ttctgctcct acggcgtcga gatctcgacg aacaggtccg
     3901 cggcccaggc ggcgaagatc gtcatcgccg agctgaaccc ccagatgccc cggaccctgg
     3961 g
//
LOCUS       DAOQTR010000158         3960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_25746_length_3960_cov_3.782074, whole genome shotgun sequence.
ACCESSION   DAOQTR010000158 DAOQTR010000000
VERSION     DAOQTR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25746_length_3960_cov_3.782074"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="PKN85_04965"
     CDS             complement(<1..1708)
                     /locus_tag="PKN85_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43788.1"
                     /translation="MQTIVRNSLISRRGLQQILSLPEEDVVYVSLLEILAKFQDIKQF
                     ASEIHTEIHLIKPTLKILGYSYESKPKYFNDSIKGPDVALFATEADRDRTSPLWGTPE
                     YYMNTLGVLLLKRFGRNLEEGVSGFYLEFENRIPSYQLFYFLKNTKTPWGILTNGKQW
                     MLMKKPLAFETRVFSVDLEEAIETNDRDALHLFCKIFSVNGLSTVVPELQEAERQSLV
                     DRLTDKKASLRNTTAGFRKKTEVFPRIVGGLSDLFAEDIFTSTRAYLTENDVYVAKRT
                     TPPNTIDEFNIADITSYLLNKKGSSPVIDPERIFLHARPEELTKDDLLAMRVLDMTPG
                     FGNVTTQLVDGIAYLSFILPYRDRNTFVAQWENEKALKRYILQRILYGIEKSHVAYDI
                     LQYAMQHRYGTEADNYRFGNPLIGMSLSDIAPHVDTRNQMGLFSKNPLDILKDVSKMY
                     RQYFSLSDKIKEDMAVKEELASQLNVYCQRIRDVMDLITATYFSKAIDERKIHESLLM
                     LDSDNASWDSHVSRDWFAEAKRIARRNGFFHLEIEFPFLVDGAFDYIFVQPSLTHIWE
                     DPF"
     gene            complement(1744..1998)
                     /locus_tag="PKN85_04970"
     CDS             complement(1744..1998)
                     /locus_tag="PKN85_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43789.1"
                     /translation="MLRVFLIVLFFLIIGTTALCAGAEPKVEVFPPASYDRHIIYQAL
                     IFFWIGIIGLVVIIAMKLREIKRVQKMGIDEEEGNVPFLD"
     gene            complement(2010..3074)
                     /locus_tag="PKN85_04975"
     CDS             complement(2010..3074)
                     /locus_tag="PKN85_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNT43790.1"
                     /translation="MSCRRLKDWFGERYDMETLGALARSKTVPSHRFDVWYFAGGLTL
                     FLFVFQFITGMALALYYVPHAEHAHKSIVDIVTKLNMGWLFRSLHHWGAQLAILVLFA
                     HVFSTLLLKAYRKPRELVWLTGFVMLAISIFFGLSGYFLLWDERAFAAVRVATGGAGN
                     LPLVGWVIKGFLRGGLDVTGETLTRFYAFHVAILPLVTIAVAGLHILLVQIHGMSVPL
                     SQERKQGGPEPFFPNLFYKDLIVWLVCLGVVVTLAVLLPPEIGRKADPLAPAPENIKP
                     EWYFLFLFQTLKLFPGEMLGMNGETIAIVIISAAILFFFLIPFFDRRSAMGQRSPLFT
                     WIGTFYLVYFTVMTVIGFLT"
     gene            complement(3055..3525)
                     /locus_tag="PKN85_04980"
     CDS             complement(3055..3525)
                     /locus_tag="PKN85_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNT43791.1"
                     /translation="MKLDHSAVTRRTFLNTLLTGWAAAVASGTLWALIKFAFPTLGKE
                     PDFVVLKRADFEGIPLNSVKPFAWGGKLGLYFKKQNGTVEALKGVCTHMECNITYKPD
                     EKKFYCACHKGWFDENGRNIEGPPPKPLEIFDIIVEGDKLIIAKKGVKVELPKA"
     gene            complement(3522..>3960)
                     /locus_tag="PKN85_04985"
     CDS             complement(3522..>3960)
                     /locus_tag="PKN85_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT43792.1"
                     /translation="IKDLVVDMTPFWRMYEKVRPWLVRRTPDPGKEIEQSEEERSRID
                     QFVNCILCACCYGACPVIARDPEYLGPAALAKLERFILDSRDERPDDFLDTVNDEKGV
                     WGCDTVLRCIEACPKDVRPTDSIVGLRKALVKKKARQLLGMKK"
BASE COUNT          818 a         1029 c         1240 g          873 t
ORIGIN      
        1 ggaaggggtc ttcccatatg tgggtgaggg agggttggac gaagatgtaa tcgaaggcgc
       61 cgtcgacgag gaaggggaac tcgatctcga gatggaagaa tccgtttcgc ctggcgatgc
      121 gtttcgcctc cgcgaaccag tcccgggaca cgtgggagtc ccaggaggca ttgtcggaat
      181 cgagcatgag gagtgattcg tgtatctttc tttcgtcgat ggccttgctg aagtaggttg
      241 ccgttatgag atccatgacg tcccgtatcc gttggcagta aacgttgagc tgtgaagcaa
      301 gctcctcttt taccgccatg tcttccttta tcttgtcgga aagggagaaa tactgccggt
      361 acatcttact gacgtccttg aggatgtcga gagggttctt cgagaagagc cccatctggt
      421 tgcgcgtatc cacgtggggg gcaatgtcgg agagcgacat tccgatgaga ggattgccga
      481 acctgtagtt gtcggcttcc gttccgtaac ggtgttgcat ggcatactgg aggatatcgt
      541 aggcgacgtg ggacttctcg ataccataga ggatcctctg aaggatatac cgcttgaggg
      601 ccttttcatt ctcccactgc gccacgaagg tgttcctgtc gcgataggga aggatgaagg
      661 agagataggc gatgccgtcc acgagctgcg tcgtgacgtt gccgaagccg ggcgtcatgt
      721 cgagcactct catcgcgagg aggtcgtctt ttgtcaattc ctccggcctt gcgtggagga
      781 atatcctttc gggatcgatg acgggagagg aacctttctt gttgagaagg taggatgtta
      841 tgtcggcgat gttgaattcg tcgatggtgt tgggaggtgt agtcctcttt gcgacgtaga
      901 cgtcgttctc agtcaggtat gcccgggtcg aggtaaagat atcctcggcg aaaaggtcgg
      961 aaagaccgcc cacgatcctg ggaaagacct ccgttttctt cctgaagccc gccgttgtgt
     1021 tgcgcaggga cgccttctta tctgtaagcc tgtcgacgag ggattgccgc tcggcttcct
     1081 ggagttcggg gaccaccgtc gagagcccgt tgacggagaa gatcttgcag aagagatgga
     1141 gcgcgtccct gtcgttcgtc tcgatggcct cctcgaggtc cacggagaac acgcgcgtct
     1201 cgaaggcgag aggtttcttc atgagcatcc actgtttgcc gttggtcagt atgccccagg
     1261 gggtcttcgt gttcttcagg aaatagaaga gctggtacga gggaatccgg ttctcgaact
     1321 cgagatagaa accgctgaca ccctcttcga gatttctccc gaagcgcttc agaagaagga
     1381 cgcccagcgt gttcatgtaa tattccggcg tcccccacag gggagacgtc ctgtcccggt
     1441 cggcttccgt cgcgaagagg gcgacatcgg ggcctttgat ggaatcattg aagtacttcg
     1501 gcttcgattc ataggagtag ccgagtatct tgagggtcgg tttgatcaga tggatctcgg
     1561 tgtgtatctc gcttgcgaac tgctttatgt cctggaactt cgcgagtatc tcgagaaggg
     1621 agacgtagac gacatcctcc tcgggaaggc tgaggatttg ttggagcccc cggcgggata
     1681 taagcgagtt acgaacaatc gtttgcatag gattgatgct acataataaa ggtttgggcc
     1741 tgcttagtca aggaaaggaa cgttcccttc ctcttcgtcg atgcccatct tctgaacgcg
     1801 ttttatctcc ctcagcttca tcgcgatgat gacgacaagg ccgatgatgc ctatccagaa
     1861 gaagataagc gcctggtaga tgatgtgcct gtcgtaggac gccgggggga agacctcgac
     1921 cttcggctcg gccccggcgc agagcgccgt tgtcccgata atgagaaaga aaaggacgat
     1981 gagaaacacc ctgagcatcg ctgtcacccc tatgtgagaa aaccgatgac ggtcatgacc
     2041 gtgaaataga caagatagaa cgtaccgatc cacgtgaaga gagggctcct ctgccccatc
     2101 gccgacctcc tgtcgaagaa ggggatgagg aagaaaaaga gtatggccgc ggagatgatg
     2161 acgatggcga tggtctcgcc gttcatgccc agcatctcgc cgggaaagag cttgagggtc
     2221 tggaagagaa agaggaagta ccattcgggc ttgatgttct ccggcgcagg cgcgagcggg
     2281 tctgccttcc tgccgatctc cggcggaagg aggacggcca gggtgacgac gaccccgagg
     2341 cagaccagcc atacgatgag gtccttgtag aagaggttgg ggaagaaagg ctcgggtcct
     2401 ccctgtttcc tttcctgtga gagaggtacg ctcataccgt ggatctggac gaggaggatg
     2461 tggagccccg ccaccgcgat cgtgacgagc ggcaggatgg cgacgtggaa tgcgtagaag
     2521 cgtgtcagcg tctcgcccgt cacgtcgagg ccgccgcgca ggaaaccctt tatcacccag
     2581 ccgacaaggg gaagattgcc ggccccgcct gtcgccacgc gcaccgcggc gaaggccctc
     2641 tcgtcccaga ggagaaaata cccgctgagt ccgaagaaga tcgatatggc aagcatcacg
     2701 aaaccggtga gccagacgag ctcccgcggt ttccggtagg ctttgagaag gagggtgctg
     2761 aagacgtggg cgaagaggac cagtatcgcg agctgcgcgc cccagtggtg gagggagcgg
     2821 aaaagccatc ccatgttgag cttggtgacg atgtcgacaa tactcttgtg ggcgtgctcc
     2881 gcatggggga cgtaatagag ggcgagcgcc atccccgtta tgaactggaa gacgaaaagg
     2941 aagagggtga gcccccctgc gaaataccag acgtcgaacc ggtgggacgg cacggtcttg
     3001 gaacgggcga gggcccccag cgtctccatg tcgtatcgtt ccccgaacca gtctttaagc
     3061 cttcggcagc tcaaccttta ctcccttttt cgctatgatg agcttgtcgc cttcgacgat
     3121 gatgtcgaag atctcgaggg gcttcggcgg agggccctct atgttcctgc cgttctcgtc
     3181 gaaccacccc ttgtggcagg cgcagtagaa ctttttctca tcgggcttgt aggtgatgtt
     3241 gcattccata tgggtgcaga cgcccttgag ggcctcgacg gtgccattct gtttcttgaa
     3301 gtagaggccc agtttgcctc cccacgcgaa gggcttcacg gagttgaggg ggatgccctc
     3361 aaagtcggcc cttttgagga cgacgaagtc aggctctttc ccgagggtcg gaaaggcgaa
     3421 cttgatgagg gcccagaggg tcccggaggc gacggcagcg gcccatccgg tgaggagggt
     3481 gttgagaaat gtccttcgcg tcaccgctga atggtcgagc ttcatttctt catccccagg
     3541 agttgtcttg ccttcttctt tacgagggcc ttcctgaggc ccacgatgga gtcggtggga
     3601 cgcacgtctt tcgggcaggc ctctatgcac cgaagcacgg tgtcgcatcc ccacacgccc
     3661 ttttcatcgt tcacggtatc gagaaagtca tcgggccgct cgtcccgcga atcgagtatg
     3721 aagcgttcca gctttgccag ggccgcgggt cccaggtatt cgggatcgcg ggctatgacg
     3781 gggcaggccc cgtagcagca ggcacagagg atacagttga cgaactggtc gatgcggctt
     3841 ctttcctcct cactctgctc tatctctttc ccggggtccg gcgtccgccg cacgagccag
     3901 ggcctcacct tctcgtacat gcgccagaaa ggcgtcatgt cgacgacgag gtcttttatg
//
LOCUS       DAOQTR010000159         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26081_length_3918_cov_3.828372, whole genome shotgun sequence.
ACCESSION   DAOQTR010000159 DAOQTR010000000
VERSION     DAOQTR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26081_length_3918_cov_3.828372"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1632)
                     /locus_tag="PKN85_04990"
     CDS             complement(40..1632)
                     /locus_tag="PKN85_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNT43793.1"
                     /translation="MLSSFFSGISGLIANSQSINVVGNNIANVNTVGFKGSRATFEDV
                     LYQSISGTSGSSQVGRGTALSSVDTSFGQGSFESTSESTDLAIGGKGFFIVRSQESQT
                     NYYTRAGQFRFDADGCMTNPAGDILQGRQIDRTTNAPYGVDTDIIISQAPSEPRATEF
                     IGMCVNLQSDADVAGTLGSLSGVANGSVTSVGLSEGKYPRVGDYTISYAAGTSTLTVQ
                     VAHRDPTGALTGTTSTYSAVVAAGTTYTNFASSGLDITTDSAMVDTASRTISFSGFST
                     DYVSATRNPTTTSNYSSSVTAYDSLGQPHVVTVYFRKAYETTTPQTSVWEWMAHLDAA
                     DSATGANDLAGWGTLIFNNNGALTSGGDATSVSFDFSQGANPAQAIDLVFGSGSGGGT
                     TTQYPIASTTNFQTQDGYPPGVLQNVTVSAEGIISGHYSNGQILNLYQITLANFNNPN
                     GLTKEGSNLYSETIESGVAYTNAPGEGGLGKISANSLEQSNVDLATEFVKMIIAQRGY
                     QANSRVITTTDEVLQELMNIKR"
     gene            complement(1649..2266)
                     /locus_tag="PKN85_04995"
     CDS             complement(1649..2266)
                     /locus_tag="PKN85_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNT43794.1"
                     /translation="MSVTSVTNTLDTTGTTSSSGSTTTTELLNTDAFMNLLVTQLKYQ
                     DPLDPMETNEFMSQLAQLTQVERLQNIYDSLTDLETTIETGNLLDMIGKKIAVDGNTL
                     SQGDEIETAPASDYDSVVFTITNVNTGVEETVTRSKGESLIYTNEGADPVTVTAYALK
                     DGSKVDCATATYRVIAGVRSTGSTVVLVAGNGDEYYASSVTTIKN"
     gene            complement(2276..3715)
                     /locus_tag="PKN85_05000"
     CDS             complement(2276..3715)
                     /locus_tag="PKN85_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43795.1"
                     /translation="MEFVLGVGMENQFFNCLFTDLKGLGKQLFPRARAGTSGEGEANA
                     FFDILSQKMGGNVNLLLSSMAAGLSADDGKAAGGEGSDTSRCPVAASLDPEEIPVLVS
                     SLISAMGQPAVTDNVSDMSEADREAITEFLEGILQILSGGDEVELTSADVMKSDAFEG
                     GGKSEEKDIKGAQDLMNCVAAVLSRLSALTREVDGMDTVASGQDGLPVERDPGPAVKV
                     ERPDPSMMKTEVQNTVPQTALSEDNEEAGFVVEIVKAAKKASVTDPSMKKEMETQPSA
                     PVSDTPDAGKRDVTVLAARTSEVHEDAGEPEKIVIRVKETMEAGMGEDQDGGDNSVMQ
                     HNDVSRQSTHQATGETKGAARNDFSSMMVEKIEKLTEHFAGRNMNMDMTVRLKIGDNE
                     TVLVGLKDEGSSITVEVRGASESTMNFLQSQKDDIARTLEGKNIQTTIHVDIDQDAEE
                     RRQQKQHRDAGQNETAEQQDFGSFFEALA"
BASE COUNT          673 a         1199 c         1118 g          928 t
ORIGIN      
        1 ggtcggtttc ccgacccccg tctttggctt aacgctgtat taccgtttga tattcatgag
       61 ttcctgcagc acctcgtccg ttgtcgtgat gacgcgggag ttcgcctggt accctctttg
      121 ggcgatgatc atcttgacga actccgtcgc gaggtccacg ttcgactgtt cgagggagtt
      181 ggcgctgatc ttgccgaggc cgccctcgcc cggcgcgttg gtgtaggcaa cgccggactc
      241 tatggtctcc gaatagaggt tgctgccttc cttcgtcagg ccattggggt tgttgaagtt
      301 tgccagcgtg atctgataga ggttcaggat ctggccgttg gagtagtggc ccgagatgat
      361 gccctcggcg ctgaccgtca cgttctggag gacacccggg ggataaccat cctgggtctg
      421 gaagttcgtc gtcgaggcta tggggtactg ggtggtcgtg ccgcctcccg agccggaacc
      481 gaagacgagg tcgatcgcct gcgcggggtt ggccccctgg gagaagtcga aggaaacact
      541 tgtcgcgtcg ccgccggagg tcaatgcacc gttattgttg aagatgagcg tgccccagcc
      601 ggcaaggtca ttggcacccg tcgccgaatc ggcggcgtcg aggtgggcca tccattccca
      661 gacgcttgtc tgcggggttg tcgtctcgta ggctttgcgg aagtagaccg tgacgacatg
      721 gggctgaccg agggaatcgt aagcggtgac cgatgacgag tagttggatg tcgtcgtcgg
      781 gttgcgtgtc gcgctcacgt agtccgtgga gaagcccgag aaggatatcg tcctcgacgc
      841 tgtgtcgacc atggcggagt ctgttgttat gtcaagcccg gagcttgcga agttggtata
      901 cgtcgtgccc gcggcaacga ccgcggaata cgtggacgtg gtccccgtga gggcgccggt
      961 gggatccctg tgcgccacct gcaccgtcag tgtgctggtg cccgcggcat aggatatcgt
     1021 gtagtcgccg accctcggat acttgccctc gctcaggccc acgctggtga cgcttccatt
     1081 cgccacgccg ctcaagcttc cgagggtgcc cgccacatcg gcgtcagact gaaggttcac
     1141 gcacatccct atgaattcgg tggccctcgg ttcgctcggt gcctgggata tgatgatgtc
     1201 ggtgtcgacg ccgtaggggg cgttcgttgt ccggtctatc tgccttccct ggagaatgtc
     1261 gcccgccggg ttcgtcatgc agccgtccgc gtcaaaacgg aactgtcccg ccctggtata
     1321 atagttggtc tgactctcct gggaccggac gatgaagaac cccttgccgc cgatggcgag
     1381 gtccgtggat tcgctcgtgc tctcgaagct gccctggccg aaggacgtgt cgacggaact
     1441 caacgccgtg ccccttccga cctgactgga gccggaggtg ccgctgatcg actggtacag
     1501 gacgtcctcg aaggtcgccc tgctcccctt gaacccgacg gtgttgacgt tcgcgatatt
     1561 attacccaca acgttgatgg attggctgtt cgctatgaga ccgctgatgc ctgaaaagaa
     1621 tgagcttaac atggtagatg cctcctcttt agttctttat cgttgtaacc gagctggcgt
     1681 agtattcgtc cccgttgcct gcaacaagga cgaccgtgct gcccgtgctt ctcactccgg
     1741 cgatgacccg gtacgtcgcg gtggcacagt cgaccttgga accgtccttc aaggcgtagg
     1801 ccgtcacggt caccgggtcg gcaccctcat tggtgtagat gagtgactcg cctttgctcc
     1861 tggtaacagt ttcctccaca ccggtgttga cgttcgtaat cgtaaagacc accgaatcgt
     1921 agtccgatgc cggcgctgtc tcgatctcgt cgccctgcga gagcgtgttg ccgtccaccg
     1981 cgatcttctt tccgatcatg tcgagaagat tgcccgtctc tatggtcgtc tccagatcgg
     2041 taagggaatc atatatgttc tggagtcttt cgacctgggt cagctgcgcc agctggctca
     2101 tgaactcgtt cgtttccatc gggtccaggg ggtcctggta cttgagctgg gtcacgagaa
     2161 ggttcatgaa ggcatcggta ttgagaagct cggtggtcgt cgtggacccc gacgatgacg
     2221 ttgttcccgt cgtatcaagt gtgtttgtga cgctcgtaac cgacatggtg cctccttatg
     2281 ccagggcttc gaagaaggaa ccgaagtcct gctgttccgc cgtctcgttc tgtccggcgt
     2341 ccctgtgctg tttctgctgc cttctttcct cagcgtcctg gtcgatatcg acgtggatcg
     2401 ttgtctggat gttcttgccc tccagggtcc gcgctatgtc gtccttctgg gactggagaa
     2461 agttcattgt gctttcgctc gcacccctga cctctaccgt gatcgacgac ccctcgtcct
     2521 tcagaccgac gaggaccgtt tcgttgtcac cgatcttcag cctgaccgtc atgtccatgt
     2581 tcatgttcct gccggcaaag tgctccgtga gcttctctat cttttccacc atcatcgagc
     2641 tgaaatcgtt cctcgccgca cccttcgttt cacccgtcgc ctgatgtgtg ctttgtctgg
     2701 agacgtcgtt gtgctgcatg acggagttgt cgccgccgtc ctgatcttcc cccatcccgg
     2761 cttccatggt ctccttgacg cgtatcacga tcttctcagg ctccccggca tcctcatgga
     2821 cttccgatgt gcgtgccgcg aggacggtaa cgtccctctt tcctgcgtcg ggggtgtccg
     2881 acaccggcgc cgaaggctgt gtttccatct ccttcttcat tgacgggtcc gtcacggacg
     2941 ccttctttgc ggccttcacg atctcgacca cgaacccggc ttcctcgttg tcctcggaaa
     3001 gggcagtctg aggcaccgta ttctggactt ccgtcttcat catcgaaggg tccggccttt
     3061 cgaccttcac agccgggccc ggatcccttt cgacggggag tccgtcctgc ccggaggcga
     3121 cagtgtccat gccatccacc tcccgcgtca atgccgagag ccgggaaagg accgccgcga
     3181 cacagttcat gaggtcctgt gctcctttta tgtccttctc ctcactcttt ccaccgcctt
     3241 cgaaagcgtc gctcttcatc acgtcggcgc tggtcagttc cacctcgtca ccgccggaga
     3301 ggatctggag gataccctcg agaaactccg tgatggcctc gcggtcggcc tcgctcatat
     3361 ccgacacgtt gtccgtgaca gcgggttgtc ccatcgctga aatgagggag gaaacaagga
     3421 ccgggatctc ctccggatcc aggctcgctg cgacagggca acgggaggtg tccgaccctt
     3481 cgccgcctgc ggccttgccg tcgtccgcag ataggcccgc ggccatcgag gacaacaaca
     3541 ggttcacgtt tccgcccatc ttctgcgaaa ggatatcgaa gaaggcattc gcctcacctt
     3601 cacccgacgt ccccgctcga gcccggggaa agagctgctt ccccagtccc ttcagatccg
     3661 taaacaggca attaaaaaac tgattttcca tgcccacccc taaaacaaat tccatgccac
     3721 aaaggcctca ttaatgattt ccagctctta caaacaccct ccgcccacgg ccgcccttgt
     3781 gcccggggaa ataatttcca cgaagaggaa ataatttcct acaagaaagc accccggcgc
     3841 gcaccacaac aaacagataa agacttgcct ctcgcccgct tcgctcaagt tcgcagagga
     3901 cgcagagaaa agattcgg
//
LOCUS       DAOQTR010000160         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26083_length_3918_cov_3.262231, whole genome shotgun sequence.
ACCESSION   DAOQTR010000160 DAOQTR010000000
VERSION     DAOQTR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26083_length_3918_cov_3.262231"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKN85_05005"
     CDS             complement(<1..615)
                     /locus_tag="PKN85_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43796.1"
                     /translation="MFVIIGAIVVLGAVFGGFVLEGGPLKLIVQPVEFLIIGGAAVGA
                     MVISAPKKLLMKIVAKVMASLKGGAINRELYLDLLRLMYEIFQVTRKDGLIALESHIE
                     HPDQSKIFTKYPNITRHHHIISFMTDTFRLIVLGGIAPHDIESLMDLDIDTHHQEGAQ
                     PGMILQKIGDSMPGLGIVAAVLGIVITMQAIGGPAEEVGHKVAVA"
     gene            complement(901..2178)
                     /locus_tag="PKN85_05010"
     CDS             complement(901..2178)
                     /locus_tag="PKN85_05010"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNT43797.1"
                     /translation="MKTLIRGGRLVDPKNSIDGRYDVLVDGPVVKEVSKSIRKVDDDT
                     RIIDASKRIVAPGLIDVHCHLREPGFEYKETIRTGTTAATRGGFTTVVCMANTNPVND
                     SRSVTEFIVDKARAEGVCKVLPCGAVTRGLRSEELSEIGEMFEAGIVAISDDGRSVRA
                     AGLLRKALEYAKIFKLPVISHCEDETLSGGCVHEGRASLLSGLDGIPAIAEELIAGRD
                     IAVARYVDAPLHLTHISTAGSVEIIERAKRTFRKITCDTCPHYFTLTDEATLTYDTNT
                     KVNPPLRSQDDVNAIKEGLRKGTIDIIATDHAPHEFTSKDVEFDLATFGISGFETALA
                     LSLGLVREDILDLKGLLMKMTANPAKLLGLEAGVISEGAPADIIVFDPDMEWTVDRKS
                     FASKGKNTPFHGMTLRGRNLFTMVDGKIVYNEL"
     gene            complement(2175..3098)
                     /locus_tag="PKN85_05015"
     CDS             complement(2175..3098)
                     /locus_tag="PKN85_05015"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNT43798.1"
                     /translation="MNWKRKDLLGIRDLSKEEMLFILDTAESFKDISKREIKKVPTLR
                     GKTVITLFYEPSTRTRTSFEIAAKRLSADTINISSSTSSSTKGESLKDTARNLESMRP
                     DAIVIRHGMPGAPHMLAGILDSSIINGGDGAHEHPTQALLDLFTMRDKKGRIDGLKVV
                     IVGDIAHSRVARSNIFSLKSFGNDVICCGPPTMIPPHIETLGVKVEYDLKRAVRKADV
                     IMMLRIQKERGGTTYIPSTKEYSTLYGLGREHVESAADDVIIMHPGPMNRGIEISDEV
                     ADGPSSVILDQVENGVAVRMAVLYLLIGGEA"
     gene            complement(3098..3628)
                     /gene="pyrR"
                     /locus_tag="PKN85_05020"
     CDS             complement(3098..3628)
                     /gene="pyrR"
                     /locus_tag="PKN85_05020"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNT43799.1"
                     /translation="MKKRVILDKKAIGRTVTRITHEILEKNAGCTELCLIGIRTRGVY
                     LSQRVHAKVTDVEGITLPIGALDITMYRDDISKLRWPEVKKTEISFDVNNMTVVLVDD
                     VLHTGRTTRAAIDAIMDLGRPKRIQLAVLVDRGERELPIHPDYAGIVCPVNENEEVLV
                     RMSEIDGKDEVVIVKA"
     gene            complement(3629..3808)
                     /locus_tag="PKN85_05025"
     CDS             complement(3629..3808)
                     /locus_tag="PKN85_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT43800.1"
                     /translation="MRPYISRASCIDCRECVSICPYEVFTEKDGTVVVETPEDCVECG
                     ACVENCRQEAIYFDD"
BASE COUNT          749 a         1105 c         1159 g          905 t
ORIGIN      
        1 ggcgaccgcc accttgtggc cgacctcttc cgccggtccg ccgatggcct gcatggtgat
       61 gacgatgccg aggacggcgg cgacgatgcc gagacccggc atggagtcac ctatcttctg
      121 aaggatcatg cccggctgcg cgccttcctg gtgatgtgtg tcgatatcga gatccatgag
      181 actctcgatg tcgtgggggg cgatcccccc gaggacgatg agcctgaagg tgtcggtcat
      241 gaaggagata atatggtgat gccgcgtgat gttggggtac ttggtgaata ttttgctctg
      301 atcggggtgc tcgatatgag attccagggc aatgaggccg tccttgcgcg tcacctggaa
      361 gatctcgtac atgagccgga ggaggtcgag atagagctcg cggttaatgg cccctccttt
      421 gagggaggcc attaccttgg caacgatctt catgaggagt ttcttggggg ccgatatcac
      481 catcgcccca acggccgccc cgcctatgat gaggaactcg acgggctgca cgatgagctt
      541 caaggggccg ccctccagaa cgaaaccccc gaataccgca cccagaacta caatcgcacc
      601 gataatgaca aacattgtgt cttccgtgcc ctcaaaagcc ccgctctctc gcggggtgta
      661 cagggtccat caccctttac ggaccagtat gctgatatca tgctgtccat cggtccgggt
      721 ataaaggtta tcggaagagg gaagaaaaga ttaagatacg gttttacgtt ttaggtttta
      781 agttttaagt gaaagaccag ggtaggcaga atagcgggat gcgcctgtat ttcaagggaa
      841 tagagccggt ttttttctgt tttcaaccta aaacttaaaa cctaaaactt gaaaccgtct
      901 ctagagttcg ttgtagacga tctttccgtc gaccatggtg aagaggttcc tgccgcgcag
      961 cgtcatgccg tggaaggggg tgttctttcc cttcgaggcg aaggactttc tgtcgaccgt
     1021 ccattccata tcggggtcga agacgatgat atcggccggg gctccttcgc ttatgacgcc
     1081 ggcctcgagg cccagcaatt tcgccggatt ggcggtcatc ttcatgagaa ggcccttcag
     1141 gtcgaggatg tcctcccgga cgaggcccag cgacagcgca agggccgtct cgaagcccga
     1201 gataccgaag gtcgccaggt cgaactcgac atccttggag gtgaattcgt gaggcgcgtg
     1261 gtcggtggcg atgatatcga tggtcccctt gcggagccct tccttgatgg cattgacgtc
     1321 gtcctgagac ctcaagggag gattcacctt cgtgttcgtg tcataggtga gggttgcctc
     1381 atcggtgagc gtgaaatagt ggggacaggt gtcgcaggtg atcttcctga acgtccgttt
     1441 cgcgcgctcg atgatctcca cgctgccggc agtcgaaatg tgggtaaggt ggaggggcgc
     1501 gtcgacgtat cgcgctaccg cgatgtccct gcccgcaatg agttcctcgg cgatggcggg
     1561 gatcccgtcg agccccgaaa gaagcgaggc ccgtccctca tgaacgcaac ccccggagag
     1621 ggtctcgtcc tcgcagtgcg atatcacggg cagcttgaat atcttcgcgt actccagggc
     1681 cttgcgcagg agcccggctg cgcgcacgga cctcccatcg tcggagatcg ccacgatgcc
     1741 ggcttcgaac atctcgccga tctcggacag ttcctcgctc cttagccccc tggtgaccgc
     1801 gccgcaggga aggaccttgc agacaccctc cgcccgggct ttgtcgacga tgaattcggt
     1861 gacgctccgc gagtcattca cgggattggt gttcgccatg cagaccacgg tcgtgaaccc
     1921 gccgcgggtc gccgcggtcg ttccagtccg tatggtctcc ttgtactcga aaccgggctc
     1981 acgcagatgg cagtggacat cgataaggcc cggcgccacg atgcgcttcg atgcgtcgat
     2041 gatcctcgtg tcgtcatcga ccttgcggat gctcttcgaa acctccttga cgaccggtcc
     2101 gtcgacaagg acatcgtatc tgccgtcgat ggagttcttc ggatcgacaa ggcgccctcc
     2161 ccgtatcagg gtcttcacgc ttcacctccg atgaggagat aaaggacggc catccgcacg
     2221 gccacaccgt tctccacctg atccaggatc accgaggagg ggccgtcggc cacctcgtcg
     2281 gagatctcga tgccccggtt catcgggccg ggatgcatga tgatcacgtc atccgcggcg
     2341 ctctcgacgt gctctcttcc aaggccgtag agggtggagt attcctttgt cgaaggtatg
     2401 tacgtcgtcc cgcccctctc tttctggatc ctcagcatca tgatgacgtc ggctttcctg
     2461 acggcgcgct tgagatcgta ctcgaccttg acccccaggg tttcgatgtg gggaggtatc
     2521 atcgtgggcg gtccgcagca gatgacgtcg ttcccgaaac tcttgaggga gaatatgttc
     2581 gaccgggcga ccctgctgtg ggctatgtcc ccgacgatga ccaccttgag gccgtcgata
     2641 cgccctttct tgtcgcgcat cgtgaaaagg tcgaggaggg cctgtgtagg gtgttcgtgg
     2701 gccccatccc ctccgtttat gatggaagag tcgaggatgc ccgccagcat gtggggcgca
     2761 cccggcatgc cgtggcggat gacgatggcg tcggggcgca tggattccag gttcctggcg
     2821 gtgtctttga ggctttcgcc tttcgtcgaa ctgctggtgc tggaggatat gtttatcgtg
     2881 tcggcgctta agcgcttcgc ggctatttcg aaggatgtgc gcgtccgggt gctgggctcg
     2941 tagaagaggg tgatgaccgt cttgccccgg agcgtgggga cctttttgat ctctctttta
     3001 gaaatgtcct tgaaggattc ggccgtgtcg agaataaaaa gcatctcctc cttagagagg
     3061 tcgcgaatgc cgaggaggtc ttttctcttc cagttcatca ggcttttacg atcaccactt
     3121 catccttgcc gtctatttcg ctcatccgga cgaggacctc ttcgttctca ttcacgggac
     3181 agacaattcc cgcataatcg gggtgaatgg gtaattccct ctcaccccgg tccacgagaa
     3241 cggcaagctg tatcctcttc ggccgtccca ggtccatgat ggcgtctatg gcggcccgcg
     3301 tcgtccggcc cgtgtgcagg acgtcgtcga cgagcacaac ggtcatgttg ttgacatcga
     3361 aggatatctc ggtcttcttg acctcgggcc acctgagctt cgagatgtcg tcgcgataca
     3421 tggtgatgtc aagggcgccg atcggcagtg tgatgccttc cacgtccgtg accttcgcgt
     3481 ggacgcgctg ggacagatag acgcccctcg tcctgatccc gatgagacac agctccgtgc
     3541 atcccgcgtt cttctccagg atctcgtggg tgatccgggt gaccgtcctc ccgatcgcct
     3601 tcttgtccag aatgaccctt tttttcattt aatcgtcaaa gtagatggct tcttgcctgc
     3661 aattctcgac gcaggccccg cactcgacgc aatcttccgg tgtctcgacc acgacggtac
     3721 cgtccttttc ggtgaatacc tcgtatgggc atatggacac acattccctg caatcgatgc
     3781 aggacgcccg ggaaatataa ggtcgcacgg tactttagta ctatagaaag ttttggagga
     3841 aaaagcaaca agaagacagg gggggtaatg tatcattctt tgtgtcagga acaaaaactg
     3901 gggtaccatc atagcgag
//
LOCUS       DAOQTR010000161         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26232_length_3903_cov_3.011954, whole genome shotgun sequence.
ACCESSION   DAOQTR010000161 DAOQTR010000000
VERSION     DAOQTR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26232_length_3903_cov_3.011954"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKN85_05030"
     CDS             <1..741
                     /locus_tag="PKN85_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNT43801.1"
                     /translation="IPDPVESDLLKAAFSRNDSGPAPDSVTEQQIEEILARTGKQDPE
                     QQLNCGACGYDSCREKAIAVALGMAEPEMCIPYMRRLAERRTDQIFSTTPNGVLMLDD
                     ELNILGMNPAFKKFFSCSDVILGRHISYLMDPAPFEKLISGVTETLDITVLHRPYNLL
                     CRELLYMLKEEKQIVGIFINITSQQEHEKKLREIRSQTVEQANELLEHQIKMAQNIAQ
                     FLGESTARGEELVRKLMSLSEGDETNPR"
     gene            722..1891
                     /locus_tag="PKN85_05035"
     CDS             722..1891
                     /locus_tag="PKN85_05035"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNT43802.1"
                     /translation="MKPTPDEPRKYIEAANAQAAKKPGGICGDYIVEERTAEATTVIV
                     ADGIGTDIKARVAAVMCASRLMELIRVGFTLREACHKVVDTMHAARTMDIPFSAFSVC
                     RVLVSGHATVVSYEIPSPILINNRLAAYLPKQRVFPMGLEMVGEVNCMLEYGDGIIMV
                     SDGVSQAGLGQQYRMGWGVQGACDYINGLLAQGMRLKDIPGRIVANVRDISGATYGDD
                     TTCSLLLCREARAINILTGPPARKMDDARVVKEFMEMKGTKIVCGSSTAEIVARNLGV
                     PIEMKNMSNAYHKPPSYDIKGVDYATEGAITLNQVYNILGEAPEKLEPDSSVSDLYRF
                     FHASDTINFLVGTAANPGHEHIVFRQMGVYPRDVIVRLLAEKLKSMGKLVNIIYL"
     gene            1947..3527
                     /locus_tag="PKN85_05040"
     CDS             1947..3527
                     /locus_tag="PKN85_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HNT43803.1"
                     /translation="MTAGAPLDFFNAADYFVDRNVRQGRGHKTAVYTEYRNYTYNDIQ
                     KMANKTANAFREVGITVDDRILVIMLDVPQFYAVFWGAMKIGAVPIPVNTMLTPEDYE
                     YYLNDSRAKVLVVSEQLLPILAQIKGDLLYLRDLVVISETKGAHIPFKQKYRHAPNTV
                     KTEFTTKDDVGFWLYSSGSTGSPKGAIHSQYDMVVTSESFAKGILGITEDDIVFSAAR
                     LFFSYGLGNGMYFPMSVGASAVLSAHAPSPENVFRHLEKFRPTIFFGVPTLYGQMLEY
                     KAKEDKERGIKKANPNDQHELSSVRVCVSAGEALPVDIFERWKKRFGIEIIDGIGSTE
                     MLHIFLSNRPGDIKPGSTGKPVPGYELRLVDESGQEVPRGEIGTLHVKGDSAAQQYWR
                     KREKTRVTMQGEWVNTGDKYYIDEDGYYWCAGRADDMLKVGGIWVSPIEVENTLSQHP
                     AVLECAVVGAPDAKNLIKPKAFVILRNGYEKTEELKEELKKWVLDRQAKFKYPRWVEF
                     VDELPKSATGKIQRFRLR"
BASE COUNT          925 a         1165 c         1126 g          687 t
ORIGIN      
        1 atccctgacc ccgtcgaaag tgacctcctg aaggccgcat tcagcaggaa tgacagcggc
       61 cccgccccgg acagcgttac ggaacagcag atagaggaga tcctggcgcg gaccgggaaa
      121 caggaccccg aacagcaact caactgcgga gcctgcggct acgacagctg cagggaaaag
      181 gccatcgccg tggctctcgg aatggcggag ccggagatgt gcatccccta catgaggcgt
      241 ctcgcggaaa ggcgcacgga tcagatcttc agcaccacgc cgaacggtgt gctcatgctc
      301 gacgacgaat tgaacatcct cggcatgaac cccgcgttca agaaattctt ctcctgcagc
      361 gacgtcatcc tgggacggca catctcctat ctcatggatc ccgccccctt cgaaaagctc
      421 atatccggcg tcacggagac cctggacatc accgtactcc accgtcccta caacctcctg
      481 tgccgcgagc ttctgtacat gctcaaggag gagaagcaga tcgtcggcat cttcatcaac
      541 ataacgagtc aacaggaaca tgaaaagaag ctcagggaga tccgttccca gacggtggaa
      601 caggcaaacg agctccttga acaccagatc aagatggccc agaacatcgc gcagttcctg
      661 ggcgaaagca cggcccgcgg tgaggaactc gtgcgaaaac tgatgagtct gagcgaaggc
      721 gatgaaacca acccccgatg aaccgaggaa atatatcgag gcggcaaacg cccaggcggc
      781 gaaaaaaccc ggcgggatat gcggcgacta catagtggag gaacgcaccg ccgaggcaac
      841 caccgtgatc gtggccgacg ggatcggtac ggacatcaag gcgcgggttg ccgccgtcat
      901 gtgcgcctcc cgcctcatgg agctcatccg cgtcggtttc acccttcgcg aggcgtgcca
      961 taaggtcgtc gacacgatgc acgcggcgcg gaccatggac atccccttct ccgccttttc
     1021 cgtctgccgc gttctcgtga gcggccacgc gaccgtcgtt tcctacgaga tcccctcccc
     1081 catcctcatc aacaaccgcc ttgcggcgta tctgccgaag cagcgcgtct ttcccatggg
     1141 cctcgaaatg gtgggcgagg tcaactgcat gctcgaatac ggcgacggca tcatcatggt
     1201 aagcgacggg gtcagccagg ccggcctggg tcagcaatac cgcatgggtt ggggcgtcca
     1261 gggggcgtgc gactacatca acggtctcct cgcacagggc atgcggctga aggacatccc
     1321 cggcaggatc gtcgccaacg tgagggacat ctcgggggcg acgtacgggg acgacaccac
     1381 gtgcagtctc ctgctctgcc gcgaggcacg ggccatcaac atcctgacgg ggcctccggc
     1441 ccggaaaatg gacgatgccc gggtggtcaa agaattcatg gaaatgaagg gaacgaagat
     1501 cgtctgcggg tcgtccaccg ccgagatcgt cgcccgcaat ctgggcgtcc ccatcgagat
     1561 gaaaaacatg tcaaatgcct atcataagcc gccgtcttat gatataaagg gtgtagacta
     1621 tgcgacggag ggtgcgatca ccctcaacca ggtctacaac atcctggggg aggcacccga
     1681 gaaactcgaa cctgactcat cagtttcaga tctctacagg tttttccacg cctccgatac
     1741 gatcaacttc ctcgttggaa cggcggcaaa tccgggacac gaacatatag tcttcagaca
     1801 gatgggtgta tacccgagag acgttatcgt caggctcctc gcggagaaac tgaaatccat
     1861 ggggaaactg gtgaatatca tatatctata gggggaacgt gaaacgggca agcgcagccc
     1921 tcccccgcac aacagaagga ggtatcatga cagccggagc accacttgac tttttcaacg
     1981 cagccgatta tttcgtggac cgcaacgtcc gccaggggcg gggccacaag acagccgtgt
     2041 acacggaata ccggaactac acctacaacg atatccagaa gatggccaac aaaacagcga
     2101 acgccttccg cgaggtcggc ataaccgtag acgaccgcat cctagtgatc atgctcgacg
     2161 taccgcagtt ctacgccgtc ttctggggcg ccatgaagat cggcgccgtc cccattcccg
     2221 tcaacaccat gctgacaccc gaggactacg aatactacct caacgacagc cgggcgaagg
     2281 tcctcgtcgt ctccgagcag ctcctgccca tcctcgcgca gataaagggc gacctcctgt
     2341 acctgcgcga cctcgtcgtc atatccgaga cgaaaggggc ccacatcccc ttcaagcaga
     2401 aataccgcca tgctcccaac acggtaaaaa cggagttcac cacgaaggac gacgtcggct
     2461 tctggctcta cagctcgggc tcgacgggct cacccaaggg tgccatccac tctcagtacg
     2521 acatggtggt cacctccgag tccttcgcca agggcattct cggcatcacc gaggacgaca
     2581 tagtcttctc ggcggcgcga ctcttcttct catatggtct cggcaacggc atgtatttcc
     2641 ccatgtccgt cggcgcctcg gccgtgctca gcgcccacgc cccgtcgccg gaaaacgttt
     2701 tcaggcatct cgagaagttc cgccccacca tcttcttcgg cgtccccacg ctctacgggc
     2761 agatgctgga gtacaaagcc aaagaggaca aggaaagggg catcaagaag gcgaacccca
     2821 acgaccagca cgaactctcg tcggtcaggg tctgcgtgtc cgccggcgag gcgctgcccg
     2881 tggatatctt cgagcgctgg aagaaacgct tcggcatcga gatcatcgac ggcatcggct
     2941 ccacggagat gctccacata ttcctgtcca acaggcccgg ggacatcaag cccggatcca
     3001 cgggaaaacc cgttcccggt tacgagctga gactcgtcga cgagagcggc caggaagtcc
     3061 cccggggcga gataggaacc ctgcacgtca agggcgacag cgccgcccag cagtactggc
     3121 gcaagcgcga gaaaacgcgg gtcaccatgc agggagagtg ggtcaacacg ggcgacaagt
     3181 actacatcga tgaggacggt tattactggt gcgccggacg tgccgacgac atgctcaagg
     3241 tgggcggcat ctgggtctcc cccatcgagg tggagaacac actgagccag cacccggccg
     3301 ttctggagtg cgccgtcgtc ggcgcccccg acgcgaagaa cctcatcaag cccaaggcct
     3361 tcgtcatcct gaggaacggg tacgagaaaa ccgaggagct gaaagaggaa ttgaagaaat
     3421 gggtactcga caggcaggcg aagttcaaat acccccgctg ggtcgaattc gtcgacgagc
     3481 ttccgaagag cgcgacgggt aagatacaga ggttcagact gaggtaaaga aggttcaaga
     3541 gttccagggt tcaagagttc aagagccttg ggacaaagat acgaaagacc ctccctgtcc
     3601 gacggatggg gagggttgct ttgccccggg aatgtcggga atttgttctt gaataacagg
     3661 tatgaccggg gttatagata tacaaagcgg atatttgagg tgtgacggaa gcgatcgatg
     3721 ccggaattgt catcggggct ttgcagcggc agagggtcga cacgggagac atggttcatc
     3781 gagagcgggg tttgccctgt tccttagatt aaagagagag ggttgagaaa tgaccagagc
     3841 gtttttcact gtggcgatcg ttttcatgtt tttcctgtcg ttccattcct tcgcggttgc
     3901 tgg
//
LOCUS       DAOQTR010000162         3893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26306_length_3893_cov_3.809536, whole genome shotgun sequence.
ACCESSION   DAOQTR010000162 DAOQTR010000000
VERSION     DAOQTR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26306_length_3893_cov_3.809536"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKN85_05045"
     CDS             complement(<1..289)
                     /locus_tag="PKN85_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43804.1"
                     /translation="MNKQAIRKAFFVCAFIGLAYAALAFNVQDRFSMKRTYETSAKKA
                     PRSLNVAVPGPQKEALRQAPVIAGDDLSFEPSLLIAIPIVAWSIREGIVEKD"
     gene            complement(286..597)
                     /locus_tag="PKN85_05050"
     CDS             complement(286..597)
                     /locus_tag="PKN85_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43805.1"
                     /translation="MHRPKKIYIESSGDALFSSVRPFVFLGFIMVFVLVVSYFLVSAH
                     NRAREDLIETLTMERELRQTHKKLATEMAGITQSRLLALKAKERLGLTKPKEEEVLVL
                     K"
     gene            complement(608..1477)
                     /gene="rsmH"
                     /locus_tag="PKN85_05055"
     CDS             complement(608..1477)
                     /gene="rsmH"
                     /locus_tag="PKN85_05055"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNT43806.1"
                     /translation="MSNQEHIAVLSREVCDNLIGTGCKVFVDATVGGAGHSIGLLERY
                     RQLQIIGIDRDKEALDRAEGTLKGYSNRVTLLRGNFRDLKQILGGIGVTSIDGILFDL
                     GISSYQLAAGRGFSFNDDEELDMRMDTRDVLTAYEIVNSYRQDEIARILYEYGEEWQS
                     RRIAKAIFDSRRKGRISTARELAGIVSSVKRKTGKIHPATKTFQALRIAVNDELGSLS
                     EGMDAAVDLLSRSGRIGMITFHSLEDRMVKQRFRGDAALTVLTRKAIRPGMDEIRSNP
                     RSRSAKLRVAEKN"
     gene            complement(1474..1914)
                     /gene="mraZ"
                     /locus_tag="PKN85_05060"
     CDS             complement(1474..1914)
                     /gene="mraZ"
                     /locus_tag="PKN85_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNT43807.1"
                     /translation="MFAGRYEYSIDDKSRVSIPAKFREALGQRYDLRLILTNLDGCIV
                     AYPYQEWLAIQDKVSAAGTIRKEARAFLRYFYSGASECPIDKLGRILIPQALKTDAHI
                     QKNVVITGQGKKIEVWAKEKWEELVRLATADPDQIADIVSELGL"
     gene            2134..2598
                     /gene="greA"
                     /locus_tag="PKN85_05065"
     CDS             2134..2598
                     /gene="greA"
                     /locus_tag="PKN85_05065"
                     /inference="COORDINATES: protein motif:HMM:NF001261.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNT43808.1"
                     /translation="MKVPITREGYEALKKEHETMLSVKRPQILKAIEEAREHGDLSEN
                     AEYDAAREEFQFLQQRVAEIEEQLRNSEIVDVRKGDGETVEFGCTVTLVNLDTDEESL
                     YRIVGPYESDIKKSTISITSPLGRALMGKSVGEEINFNAPGGKRTYEIVKVD"
     gene            complement(2719..>3893)
                     /locus_tag="PKN85_05070"
     CDS             complement(2719..>3893)
                     /locus_tag="PKN85_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT43809.1"
                     /translation="PKFQQHGHTMKSLELMSPDSRDDSIGIESIVERAKAAPVVKLVD
                     LMLWQAIDKRASDIHIEPFENQVSLRYRIDGKLYEMAPPPKHLHLPIVSRIKILSGLD
                     IAEKRLPQDGTFMVKLKDRTIDLRVSVLPTIYGEKAVLRILDRTGVVFLTGPTGSGKS
                     TTLYAILQEIRSSEKNILTVEDPVEYRLAGINQVQVKPEIGLTFASALRAFLRQDPDI
                     MLVGEVRDLETAEICVRSALTGHLVLSTLHTNDAPSAVTRLIDIGVEPYLLAPTVLAV
                     VGQRLVRKLCPECKEAYRPTTKDLGSVKLNADTIYRAAGCPACNNTGYRGRTLIAEIM
                     SSSEELKSLITHTVSYQNMRETARKLGMTTLYESGLKKVEKGITSLEEVMSVTFGI"
BASE COUNT          751 a         1103 c         1120 g          919 t
ORIGIN      
        1 cgtccttctc cacgatcccc tccctgatcg accaggcgac gatgggaatt gcgatgagca
       61 gcgacggttc gaaagaaagg tcgtccccgg cgatcaccgg cgcctgccgg agcgcttctt
      121 tctgtggccc ggggacggcg acgttcaggc tccggggagc tttcttcgcc gatgtctcgt
      181 aggtcctttt catcgaaaac ctgtcctgaa cattgaaagc cagcgcggcg tatgcgagcc
      241 cgatgaatgc gcacacgaag aaggctttcc tgatcgcctg tttgttcatt tcaacacgag
      301 aacctcttcc tcttttggct ttgtgagccc caggcgctcc ttggccttga gcgccagaag
      361 tctcgattgt gttatacccg ccatctccgt tgccagcttc ttatgggtct gcctcaattc
      421 acgttccatg gtaagcgtct cgatgaggtc ctcccgggcg cggttgtggg cgctgaccag
      481 gaagtagctg acaacgagga cgaagaccat gatgaacccg aggaaaacga agggtctgac
      541 gctcgagaag agcgcgtccc ccgatgactc gatgtatatc ttctttggtc tgtgcatagg
      601 gcccccgtca gttcttttct gcaacacgaa gcttggcgct gcgcgagcgc gggttgcttc
      661 gtatctcgtc catccccggc ctgatggcct tccgtgtcag cacggtcagc gcggcgtcgc
      721 cgcggaaacg ctgtttcacc atgcggtctt ccagggaatg gaatgttatc atgcctatcc
      781 tgccgctgcg ggaaaggagg tccacggccg cgtccatccc ttccgaaagg ctccccagct
      841 cgtcgttgac ggcgatgcgg agcgcttgga atgtcttcgt ggcggggtgt atcttccccg
      901 tctttctctt gacggaggaa acgatgccgg ccagctcccg cgccgtgctg atcctgccct
      961 tcctgcggct gtcgaagatg gccttcgcta ttctgcgcga ctgccattcc tccccgtatt
     1021 catagaggat ccgggcgatc tcgtcctgcc ggtaggagtt cacgatctca tatgcggtga
     1081 ggacatccct cgtgtccatt ctcatgtcga gctcctcgtc gtcgttgaag ctgaagccac
     1141 gccctgcagc cagctggtag cttgatatac cgagatcgaa gaggatgccg tctatggacg
     1201 tcacaccgat cccccccagt atctgcttca agtccctgaa gtttcccctc agtagtgtta
     1261 cccggttgct ataccccttg agggttccct ctgcccggtc gagtgcttcc ttatctctat
     1321 caataccgat gatctgtaat tgtctgtatc tttccagcaa gccgatggaa tgtcccgctc
     1381 cgccaacagt ggcgtcgacg aagaccttgc atccggtgcc gatcaggttg tcacatactt
     1441 ctctggaaag aacggctatg tgttcctggt tgctcaaaga ccgagttccg atacgatatc
     1501 agctatctga tcggggtcgg ccgtggccag ccttaccagt tcctcccatt tttcctttgc
     1561 ccagacctct atcttcttcc cttgcccggt gatgacgaca ttcttctgaa tgtgagcgtc
     1621 cgttttcaac gcctggggga tgaggatccg tcccagcttg tcgatggggc actccgatgc
     1681 cccggagtag aaataacgca gaaaagcccg cgcctccttt cggatagtcc ccgcggcgga
     1741 aaccttgtcc tggatggcca gccactcctg gtagggataa gcgactatgc aaccgtccag
     1801 gttggtgagg atgagcctga ggtcgtacct ctgccccagg gcttccctga acttcgccgg
     1861 gatgctcacc ctgcttttgt catcgatcga atattcgtag cgtcctgcga acaatctccc
     1921 acctttttac accttttacc actttagtca tttatacttg taactccata tcttgtcaag
     1981 gtttttttcc cccatctgcg ccgtctggcg cttctctttc gggccagtcc ccgagggtgt
     2041 cagaagaggt ggggtggatg ctgacacaaa tctgttgata atttgccccg cctcaggtag
     2101 tcttttactc catcgcgaat aatcggagcg tcaatgaagg taccaattac cagggaaggc
     2161 tatgaagcac tgaagaaaga gcatgagacc atgctcagcg taaaacggcc ccagatcctc
     2221 aaggccatag aggaagcgcg ggagcatggc gacctttcgg agaacgcgga atacgatgcc
     2281 gccagggaag aatttcagtt tcttcagcag agggttgccg agatcgagga gcagctgaga
     2341 aattccgaga tcgtcgacgt gaggaaaggc gatggagaaa cggtcgagtt cggctgcacc
     2401 gtgaccctcg tcaatctcga caccgatgaa gaatcccttt acaggatcgt cggcccctac
     2461 gaatcggaca tcaagaaaag caccatatca ataacctcac ccctcggcag ggccctcatg
     2521 ggcaagtccg tcggtgaaga gatcaacttc aacgcccccg gtggcaagag aacgtacgaa
     2581 atagtgaaag tagactaaag gcggcttgaa tggcttgagc cgcttgaatg ttgaaaacaa
     2641 aagaccatct cttcattgga gaaggtctct ttttttactc tcaagcgact caagccattc
     2701 aagcgactca agccgtcttt agattccgaa tgtcacgctc attacttctt cgaggctcgt
     2761 tattcctttc tcgacctttt tgaggcctga ttcgtagagg gtggtcatgc ccagtttgcg
     2821 tgcagtctcg cgcatgttct ggtaggagac agtgtgcgtg atgagcgatt tgagctcctc
     2881 cgaggaggac atgatctcgg cgatcagtgt gcgcccacga taacctgtgt tgttgcaggc
     2941 cgggcatccc gcagcccggt agatcgtatc tgcgttgagc tttacggagc ccaggtcttt
     3001 cgtggtgggt ctgtaggctt ccttgcattc ggggcagagc ttcctcacga gtctctgacc
     3061 gacgacggca aggaccgtcg gtgcgagcag gtagggttcg acgccgatat cgatgagcct
     3121 tgtcaccgcc gacggggcgt cgttggtgtg gagcgtggag agcaccagat gacccgtcag
     3181 cgccgagcgg acgcagatct ccgccgtctc gaggtcgcgg acctcgccaa cgagcatgat
     3241 gtcggggtcc tgtcgcagga aggccctcaa ggccgacgcg aaggtgagcc ctatctcggg
     3301 cttgacctgc acctggttga tgccggccag ccggtactcg acggggtcct cgacggtgag
     3361 gatgttcttc tccgacgacc tgatctcctg gaggatggcg tagagggtcg tcgactttcc
     3421 gctgcccgtg gggccggtga ggaagacgac ccccgtcctg tcgagtatgc gcagtacggc
     3481 cttctcgccg tagatggtgg gtagcacgga gacgcgaaga tcgatggtcc ggtccttgag
     3541 tttcaccatg aacgtcccgt cctggggaag ccgtttttcc gctatgtcga ggcccgagag
     3601 gatctttatc cgggaaacga tggggaggtg aaggtgcttc gggggtgggg ccatttcgta
     3661 gagtttgccg tcgatccggt acctcaggga cacctggttc tcaaagggct cgatgtggat
     3721 atcggaggcg cgcttgtcga tggcctgcca gagcatgagg tcgacgagct tcaccacggg
     3781 ggcggccttg gcgcgctcga cgatgctctc tatgccaatg gaatcgtccc gggaatcggg
     3841 ggacatgagc tcgaggcttt tcatggtgtg gccgtgctgc tggaatttcg ggg
//
LOCUS       DAOQTR010000163         3876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26466_length_3876_cov_4.068045, whole genome shotgun sequence.
ACCESSION   DAOQTR010000163 DAOQTR010000000
VERSION     DAOQTR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26466_length_3876_cov_4.068045"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..578)
                     /locus_tag="PKN85_05075"
     CDS             complement(63..578)
                     /locus_tag="PKN85_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNT43810.1"
                     /translation="MLIRLTEIEDILVVKGEMDTARFPKTDDAEFTLLEPVRYELTVA
                     KFDDTVSIEGPVTFKASLACSRCLESYSLSMSLQMAIKLTPKSMLPDAAEMELHNDDL
                     DVYYYEGDEIDLDPFIYEEVMLNIPVRPLCSEECKGICPTCGRNRNAEPCDCPEAPSS
                     LLGEKLKSFLN"
     gene            complement(731..994)
                     /locus_tag="PKN85_05080"
     CDS             complement(731..994)
                     /locus_tag="PKN85_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNT43811.1"
                     /translation="MNVEIRVIPNAKKRAIARTPAGITVRLTALPLEGKANDELIRYL
                     SDVLKVKRSAIRILKGQRDRHKVLELPIDEKELETLLGGPREG"
     gene            complement(1005..1301)
                     /locus_tag="PKN85_05085"
     CDS             complement(1005..1301)
                     /locus_tag="PKN85_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNT43812.1"
                     /translation="MSAFGVFIVWIAKILNMALTIYFWVIFARALFSWIRPNPYNPIV
                     RTVVRLVDPVTNRIRRVLPTRFGMIDVAPFFLMLIIIFLQEAITRLLLHLALRL"
     gene            complement(1534..2811)
                     /locus_tag="PKN85_05090"
     CDS             complement(1534..2811)
                     /locus_tag="PKN85_05090"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNT43813.1"
                     /translation="MKSTFFIHTTGCRANQWDSSVIAGNLQGAGYAPTGADRAEIVVI
                     NACTVTEGAVRDIRRFINRLRRENPGAKVVVAGCHGQAYPHDSFGADLVLGQAEKLSP
                     DRYAAAAGIFVSSRESLVMDEAPEDPAMTGKTRFFFKIQDGCDRFCSYCIVPYARGAP
                     RSRPLREILRTMETLGRRGVQEVVLTGIEISAWHDPGGGTGLTDLIAILEDAPTPPRI
                     RLSSVDPLMFDMGFIETAASSRKLARSFHIPLQSASDRVLAAMRRPYRQEDVRGMIDR
                     ILARMPDAGIGMDVIAGFPGESEDLFEETRAFLESCPVYYLHVFPYSSRPGTAAALMD
                     GTVPRGTAKARVRILKDIDARKRETFHRRFIGSLARIVPEGKRCLGRYMRGYTNNYIP
                     VHIPYDRKLENRMVEVRMDRVEGGIVLGEPIIQ"
     gene            complement(2949..>3876)
                     /locus_tag="PKN85_05095"
     CDS             complement(2949..>3876)
                     /locus_tag="PKN85_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT43814.1"
                     /translation="IKGIAYGAGKGHIITSAVEHPAVLTCCRYLETRGFPVTYLPVDG
                     HGLVDPADVKDAIRKDTILISIMYANNETGTVMPIADIARVAREREIPFHSDMVQALG
                     KIDVDVTALGVDLASFSGHKVYAPKGVGALYIREGLALDNLIHGGHQESGRRAGTENV
                     IGIVAFGRACELAREEMNERRETVEGLRRRLLNGILERIDQVRLNGHPLQRVPNTLNL
                     SFEFIESESLLLALDMKGVAVSSGSACSSGSSEPSHVLTAMGIPGPLCQSALRFSLGC
                     ENSPADVDYVLDILPGLVEKLREMSPFYKKTF"
BASE COUNT          795 a         1112 c         1122 g          847 t
ORIGIN      
        1 gtctcttgtc ccgtctcgcc ttcgcagttt ttcgttttgg tacggccatg gtctactcct
       61 tgtcagttta agaaagattt caatttttcg ccgaggaggc tcgagggcgc ctcgggacaa
      121 tcgcagggtt ccgcgtttct gttcctgccg caggtcgggc atatcccttt gcattcttcg
      181 ctgcacagcg gcctcacggg gatgttgagc atgacctctt cgtagatgaa gggatcgagg
      241 tcgatctcgt cgccttcgta gtagtagacg tcgaggtcgt cgttgtgaag ctccatctcc
      301 gccgcgtcag gaagcatgct tttgggggtg agcttgatgg ccatctgaag ggacatcgag
      361 aggctgtagc tctcgaggca gcgggaacag gccagcgatg ccttgaacgt gacgggtcct
      421 tcgatgctga cggtgtcgtc gaactttgcg acggtaagct cgtagcgcac cggctcgagc
      481 agcgtgaatt cggcatcgtc agtcttcggg aatcgtgcag tgtccatctc acccttcacg
      541 accagtatgt cttctatttc cgtaagtcgt atcaacacgg gtcgccttct tttgaagata
      601 gataatataa agagtttacc attttcttgt caaggaaatt aacctgttgt aactcttgcc
      661 tttttgttgg aagaaggctg tggagctgtt gtcttggcgc aaaggagggg agtggcccca
      721 tcgcccggaa ttacccctca cggggcccgc ccaggagggt ttcaagctct ttttcatcaa
      781 tggggagctc gagcaccttg tgcctgtcac gctggccctt aaggatcctg atggccgatc
      841 gtttgacctt gaggacgtcc gacaggtacc ggatcagctc gtcgttcgcc ttgccctcga
      901 ggggcagtgc cgtgagcctg acggtgatgc ccgccggagt gcgggctatc gccctctttt
      961 tcgcgtttgg tatcaccctg atctccacgt tcatggcggg ctccctagag cctgagggca
     1021 aggtggagca ggagcctcgt gatcgcctcc tggagaaaga tgatgatgag catgagaaag
     1081 aaaggggcga cgtcgatcat gccgaaccgg gtgggcagga ccctccttat gcggttggtg
     1141 acgggatcga cgagcctcac cactgttcgc acaatgggat tatacgggtt tggccgtatc
     1201 cacgagaaga gcgcccgcgc gaagatcacc cagaaataga tggtgagggc catgttgagg
     1261 atcttggcga tccagacgat aaagacaccg aaagcggaca ttgacaactc ctgacaaaat
     1321 taatgaccaa ttgaccaaat tacaataacc aggtaaaaga acaatgacca ataaaggata
     1381 atgaccaatt gaccaaatta caataaccaa atgaaaggat aataaacaat gacgaaattc
     1441 caatgaccaa tgaaaaagat aatgaccaat ttaccaataa ccaattgaac agatagaaag
     1501 gcacaacgga ctgcccgtat cgcttttcgt tggtcattgt attatcggtt ctcccaggac
     1561 tattcccccc tccaccctgt ccatcctcac ctcgaccatc ctgttctcaa gcttcctgtc
     1621 gtaggggatg tggacgggaa tgtaattgtt cgtgtatccc cgcatgtagc ggccgaggca
     1681 gcgcttgccc tcggggacga tccgcgccag gctgccgatg aagcggcgat ggaaggtttc
     1741 ccgctttctc gcgtcgatgt ccttgaggat cctcaccctg gccttcgcgg tccctcgagg
     1801 tacggttccg tccatgaggg cggccgccgt ccctggccgt gaggaatagg ggaagacgtg
     1861 gaggtagtag acggggcatg attccaggaa tgcccgggtt tcctcgaaga ggtcctcact
     1921 ctcaccgggg aaccccgcta tcacgtccat gccgatcccg gcgtcgggca tccttgcgag
     1981 gatcctgtcg atcatccccc tcacatcctc ctgacgatag gggcgccgca tggcggcgag
     2041 cacgcggtct gacgcgctct gcagggggat gtggaagctc cttgccagtt ttcgggagga
     2101 ggcggccgtt tcgataaagc ccatatcgaa catcaggggg tcgacggagc tgaggcgtat
     2161 ccttggcggg gtgggggcat cctcgaggat ggcgataaga tccgtgaggc ctgtccctcc
     2221 cccagggtcg tgccacgccg atatctcgat ccccgtcagg accacctcct ggacacctct
     2281 gcgccccagc gtctccatgg tgcgcagtat ctccctcagc ggcctgctcc gcggcgcgcc
     2341 tctcgcatac ggtacgatgc agtagctgca aaacctgtcg cacccgtcct ggatcttgaa
     2401 aaagaaccgc gtctttcctg tcatggccgg atcctccggt gcctcgtcca tgacgaggga
     2461 ctcgcgagaa ctgacgaaaa taccggccgc tgccgcatac cggtccggag acagcttttc
     2521 cgcctgtccc aggacgagat ccgccccgaa gctgtcatgt ggataggcct gtccgtgaca
     2581 gccggcaaca accaccttcg cgcccgggtt ctcccttctc aggcggttta tgaaacgccg
     2641 tatgtccctg acggcgccct ccgtaacggt gcaggcattg atcaccacga tctcggcccg
     2701 atcggcgccc gtcggcgcgt aaccggcccc ctgaaggttc cccgcgatga ccgaagaatc
     2761 ccactgattg gccctgcagc cggtggtgtg gatgaaaaag gtcgatttca taataataat
     2821 aaggacagtt ttaggtttta ggttttaagt gaaagacgaa cagaaactgg cgggatgctg
     2881 cggggcgacg cagcccgaaa caggaaccgg ttcttcctgt ttttcactta aaacctaaaa
     2941 cgtaaaactt aaaacgtctt cttataaaac ggtgacatct cccgcagctt ttccacgagg
     3001 cccggaagga tatcgaggac gtaatccacg tcagcgggac tgttctcgca tcccaggctg
     3061 aagcgcagcg cgctctggca caggggcccc ggtatgccca tggccgttag gacatgggag
     3121 ggttccgagg agcccgacga acaggccgaa ccggacgata cggcaacgcc cttcatgtcg
     3181 agggcaagca gaagcgattc cgattctatg aactcaaagc tgaggttgag agtgttgggg
     3241 accctctgca ggggatgtcc gttgaggcgc acctgatcga tgcgctccag gatgccgttg
     3301 aggaggcgcc gccgcaggcc ttcgacggtc tcccgccgct cgttcatctc ctctctcgcc
     3361 agttcacagg ccctgccgaa ggccacgatg ccgatgacgt tctccgtccc cgcccgtctt
     3421 cccgattcct gatggccgcc gtggattagg ttgtcgaggg cgagaccctc gcgtatgtac
     3481 agggctccca cccccttcgg ggcgtacacc ttgtgccccg agaaactggc gaggtcgacg
     3541 ccgagggcgg tcacatccac atctatctta cccagggcct gcaccatgtc cgaatgaaag
     3601 ggtatctctc tctcgcgggc gacgcgggcg atatcggcga tgggcatcac tgtccccgtt
     3661 tcgttgttcg cgtacatgat ggagatgagg atcgtgtcct tcctgatcgc gtccttcacg
     3721 tccgccgggt cgacaaggcc atgtccgtcg acgggaagat aggtgacggg aaagccacgg
     3781 gtctcgaggt acctgcagca ggtgagcacg gcggggtgtt ccaccgccga cgttatgatg
     3841 tggcctttgc ccgccccgta ggcgataccc ttgatg
//
LOCUS       DAOQTR010000164         3870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26535_length_3870_cov_3.104587, whole genome shotgun sequence.
ACCESSION   DAOQTR010000164 DAOQTR010000000
VERSION     DAOQTR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26535_length_3870_cov_3.104587"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKN85_05100"
     CDS             <1..569
                     /locus_tag="PKN85_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNT43815.1"
                     /translation="TERVVVVGASTGGTEALKSFLEALPLDAPGMVIVQHMPEHFTAA
                     FAKRLDGICRVSVKEARNDDTVVRGRVLIAPGNKHTLLKRSGARYYVEVKDGPLVSRH
                     RPSVDVLFRSAARYAGKNAVGVIMTGMGDDGAKGMLEMKDAGAYTVAQDEATCVVFGM
                     PKEAIKVGAVKTVAPLGHIAGIVMRESG"
     gene            complement(667..2604)
                     /locus_tag="PKN85_05105"
     CDS             complement(667..2604)
                     /locus_tag="PKN85_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43816.1"
                     /translation="MNACKVLKNKDTVHGTISAELLGETALALINATDELVVLAGGNG
                     NIIALNKKFAARYETGGESLVGSCVYDVLTGDLAGKTRDVALKVLKDGKTAKFEAKTD
                     GSWMEVEIYPIPDSQENIGKVAIFMRDVTRERRYEEALRESEKKYRQIVNTASEGICV
                     ADEEFRITFVNAQALKMFGYSEEEMLGKSLDDFIAEEDLEDQSEKKQRRQEGISDQYE
                     RRFRRKDGQSLWVNISASPILNRKNRFVGCISLFTDITTRKQMENELLNFRKLESVGI
                     LAGGIAHDFNNLLMSMLGYISLARMYLSEGESKARDKLTEAERTIDRAKELTTQLLTF
                     SKGGSPLKKTLHLQPIVREVSRISLSGSRIKCKYSFQDNVWPVKADETQIRQVIHQLV
                     RNSREAMPEHGGSVFISVRNRTLQAEPGTFLPGGDYVELSISDTGNGIAKEHLSHIFD
                     PYFTTKDFGPQKGMGLGLAVCYSVVKKHGGQIEVESEAGEGATFNVYLPAHRTDPVKE
                     EPDRAGAEPASESSLKVLIADDEQSILQTTTMLLTHLGHDVTGVGDGRQAIDLYRSSK
                     EAGFPFDVVILDLISPSGPDGVQILQELLGIDPGVSAILSSGYPNDPQIVNYKEHGFK
                     GVLVKPYRIEDLNLALKLARG"
     gene            complement(2591..3283)
                     /locus_tag="PKN85_05110"
     CDS             complement(2591..3283)
                     /locus_tag="PKN85_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43817.1"
                     /translation="MTAPAKRSKTVILRKDRVEESLEASRRPSARVLIAADDGSILHF
                     LESAIGSWGYETMVVFNDRELLYRMEREERPALVILDGSMQGVDSLVLCRRIRLSPDM
                     TRPYIILLVPDVTDRQSFPDPAWGPDDLLPNPCTRTELHAHVRTGMRVIDLETRVSRL
                     VTDLARAANRIERLSGLLPICSHCKKIRDDSGYWNELEKYISDHSDAEFSHAICPSCL
                     AKYYPDVKDECL"
BASE COUNT          783 a         1068 c         1138 g          881 t
ORIGIN      
        1 caacggagcg tgtcgtcgtc gtgggtgcgt cgacaggcgg aacggaggcg ctcaagtcgt
       61 ttttggaggc ccttcctctt gacgctccgg gaatggtcat cgtccagcac atgccggaac
      121 acttcacggc cgcctttgcc aagcggctcg atggcatttg ccgggtttcc gtcaaggaag
      181 cccggaacga cgacactgtg gtccggggac gggtcctcat cgctccgggc aacaagcaca
      241 ccctcctcaa gagaagcggc gccaggtact atgtcgaggt gaaggacggg ccgctggtat
      301 cgcgtcacag gccttccgtc gatgtcctgt tccggtcggc ggcgcgctat gccgggaaga
      361 acgccgttgg cgtcatcatg acagggatgg gtgacgacgg agccaagggg atgctcgaaa
      421 tgaaggatgc cggcgcttac acggtggccc aggacgaagc gacatgcgtc gttttcggga
      481 tgcccaagga ggccatcaag gtgggcgcgg tcaagacggt agctcccctc ggccacatag
      541 ccggtatcgt gatgagagag agcgggtaga aagagagagg acaggtgata ggtaatgggt
      601 cataggtgat gggagaaagc aatctctccc tatcacccat tacctatcac ccattacccg
      661 tcttctctat cctctggcca gtttcagggc caaattcaga tcttctatcc tgtatggttt
      721 tacgagaaca cccttgaagc cgtgctcctt gtagttgact atctggggat cgttcgggta
      781 gccgcttgag aggatggccg aaacaccggg atcgataccg agaagttcct ggagtatctg
      841 gacaccgtca ggaccgctgg gggagataag gtcgaggatc accacatcga agggaaaacc
      901 tgcctccttc gagctgcggt aaagatcgat ggcctgcctc ccgtcgccca cgcccgtgac
      961 gtcatggccg aggtgggtga gcagcatcgt cgtcgtttga aggatggatt gctcatcgtc
     1021 ggcaatgagc accttgagag aggactcact cgcgggttcg gccccggccc ggtcgggctc
     1081 ctccttcacc ggatcggtcc tgtgcgccgg gaggtacacg ttgaacgtcg ccccctcacc
     1141 ggcctccgac tccacctcta tctggccgcc atgtttcttt acgacggagt agcagacggc
     1201 gaggccgaga cccattcctt tctgtggacc gaaatccttc gtggtgaaat agggatcgaa
     1261 gatgtgtgag agatgctcct tcgcgatccc gttgcccgtg tccgagatgg agagctcgac
     1321 atagtccccg cctggcagga acgttcccgg ttcggcctga agcgtcctgt tccggacgga
     1381 gatgaacacc gagccgccgt gctccggcat ggcctcccgg gagttccgga cgagctgatg
     1441 aatgacctgc cggatctgcg tctcatctgc cttgacaggc cacacattgt cctgaaagct
     1501 gtatttgcac tttattcgtg aaccggacag ggagatccgg gagacctcgc gaacgatggg
     1561 ctgcagatgg agcgtcttct tcagggggct tccgcctttc gagaacgtga ggagctgtgt
     1621 ggtcagttcc tttgccctgt ctatggtcct ttcggcctcg gtgagcttgt cccgggcctt
     1681 tgattcgccc tcggacaggt acatcctggc gagggagata tatcccagca tggacatgag
     1741 gaggttgttg aaatcgtgtg cgataccccc ggcaagaatg cccacggact cgagcttcct
     1801 gaaattgaga agctcgttct ccatctgctt gcgtgtcgtg atatccgtaa aaagggatat
     1861 gcatcccaca aagcggttct tcctgttcag gatggggctg gcggagatgt tgacccagag
     1921 ggattgcccg tcctttcttc tgaagcggcg ttcgtactgg tccgatatcc cttcctgtct
     1981 gcgctgcttc ttctcggact ggtcctccag gtcctcttcc gctatgaagt cgtcgaggga
     2041 cttcccgagc atctcttcct cggaataacc gaacatcttc agggcctgag cattgacgaa
     2101 ggtgatcctg aactcctcgt cggccacgca aatgccctca ctcgctgtgt tgacgatctg
     2161 gcggtacttc ttttcgcttt cccgaagggc ctcctcgtac cggcgctccc tggtgacgtc
     2221 ccgcatgaat atggcgacct tcccgatgtt ctcctgactg tccggtatgg gatagatctc
     2281 cacttccatc catgaaccat cggtctttgc ctcgaatttg gcggtctttc catccttgag
     2341 gaccttgagg gccacatccc ttgtttttcc cgcaaggtca ccggtgagaa cgtcatagac
     2401 gcaggaaccg acgagcgact cgccccccgt ctcgtaccgg gcggcgaact ttttgttgag
     2461 cgctatgatg ttgccgttcc cgccggcgag gaccaccagc tcatcggtgg cattgatgag
     2521 ggctagggcc gtttccccga ggagttccgc ggatatggtg ccgtggaccg tatccttgtt
     2581 cttcagcacc ttacaggcat tcatccttca catccgggta gtacttggca aggcaactgg
     2641 ggcaaatggc gtggctgaac tcggcgtcgg aatgatcgct gatgtatttc tccaattcgt
     2701 tccagtatcc cgagtcatcg cgtatcttct tgcaatggga gcatatggga aggagcccgc
     2761 tgagcctttc gatcctgttc gccgcgcgcg cgagatcggt gacgagccgt gacaccctgg
     2821 tctcgaggtc tattacgcgc atgcccgtcc tgacatgggc gtgcagctca gttctcgtgc
     2881 aggggttcgg aaggagatcg tcgggtcccc atgcggggtc cggaaacgac tgtctgtcgg
     2941 tcacatccgg tacaagaagg atgatatagg gccgcgtcat gtcgggagac agacgaatac
     3001 gcctgcacag gaccagactg tccacgccct gcatggaacc gtcaaggatg acgagagcgg
     3061 ggcgctcttc ccgttccatg cggtacagaa gctccctgtc gttgaaaacg accatggtct
     3121 cataacccca actcccgatg gctgattcaa ggaaatgcaa gatcgagcca tcgtcggccg
     3181 ctatgaggac gcgggccgac gggcgtcgcg acgcctcaag gctctcctcg acgcggtcct
     3241 tgcgcaggat gaccgttttg cttctctttg ccggggcggt catgcagaaa gggagaggag
     3301 gatgttggtt gtctcatcaa ggatgctcgc gccgtaaaca tcctcctctt gaatcgccgc
     3361 atgcatataa ccggtcgtac tgaggtctat tggggggtgg cgaagcttct gtggcagggg
     3421 gaaagtcaag tagatgtatt ggggggcatg atctacctgt cttccgttgc taatggcgag
     3481 tttgaaatgg gaaacgggga tgtctcccga tccctgcgcg caagaagctt cgccggtgga
     3541 ggtcatcatt agatcacatg tggcataagg atcaaccatg acattccata tagcaatacc
     3601 tgtgccaatg cgtaatacat tgatatcatt ggggaggcac agatacagac acctggcatt
     3661 gtggaatcgg caccataatt atggaatcaa ttccatacta aaagtcacat aaacgaatgg
     3721 ttcaagagtt caaggggaaa gaccctggaa agaggctggt cgtcaacaca atcctggctg
     3781 gtgcatgcaa acgaatcttc tttctgtttt ttgacccttg tatgttactc cccatggaat
     3841 atcacaggcc gtccatgata agaagaatga
//
LOCUS       DAOQTR010000165         3868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26546_length_3868_cov_2.840283, whole genome shotgun sequence.
ACCESSION   DAOQTR010000165 DAOQTR010000000
VERSION     DAOQTR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26546_length_3868_cov_2.840283"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="ligA"
                     /locus_tag="PKN85_05115"
     CDS             <1..1046
                     /gene="ligA"
                     /locus_tag="PKN85_05115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNT43818.1"
                     /translation="IEAETTVEDILVSVGRTGALTPVASLNPVTVGGVEVSRATLHNE
                     DEIARKDVRVGDRVIVTRAGDVIPEVVRVLDAARPGRTEPFRMPEKCPVCGEAVVRPP
                     GEAIRRCVNISCPAQIKGSIEHFASKRAMDIDGLGTKLVEQLVDRQVIRDVSDLYYIR
                     KETLAGLDRMADKSAGNIIDAIEASKHRPFARFLNALGIRQVGEHAADLLAERFDSIE
                     KLMAADTQMLLAIPEIGPEAAGSITAFFRDAKNRETIKRILDAGVKLEYEQGGVKKLA
                     GLTFVFTGSLSSMSRDEARAKVEDLGAKTASSVSKKVSYVVAGEEAGSKLDKARQLGL
                     TILTEEEFLALLT"
     gene            1173..1673
                     /locus_tag="PKN85_05120"
     CDS             1173..1673
                     /locus_tag="PKN85_05120"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HNT43819.1"
                     /translation="MKRVISLSGLFCLAVLIVATGLSADAVAGTKTGNLEVTATCSAS
                     CEFLTQGTNLAFGPHDPLGGTETTAQASWSYRCVAGTKYKLYIDGERQMLLDGTDSGN
                     KLAFQLYKDDYSTVFPESESTAGELTAESNAETPFTINGKIAAGDGQSLKAGSYSKTL
                     TVTLLY"
     gene            1681..2484
                     /locus_tag="PKN85_05125"
     CDS             1681..2484
                     /locus_tag="PKN85_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HNT43820.1"
                     /translation="MSRTDTLTWPVRKTGHRPVLPFILLAAIVLLSCAPASAQSFSVH
                     PTRVSFDRTTKAQKLIIRNKGATDLSLQVGAFKWSQNKDGRDVYEDTSDIVVFPPIFK
                     IRKGEQRLVRLGTTLGPTSREGAYRVFVEELPVADPKRSGPGINLVFKVGIPLFVSPL
                     QASREALGICLAIEEGTVVLSALNNDNAHAEIESVSIIGQDSREKEIFSKGFNGWYIL
                     SGSSRVYKVDIPPAICKRLASVHAVVRTARNSFEDVVEVTGGMCGPQTR"
     gene            2463..>3868
                     /locus_tag="PKN85_05130"
     CDS             2463..>3868
                     /locus_tag="PKN85_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HNT43821.1"
                     /translation="MWAADQMILPWLRAGAALVALLIPLFLAVPAPCGDRQHVVLEVI
                     LNTDNKGEFFVYLTADGDCLMSRDDLVAIGFDGPAGTILQTGGRTFISLRSMKDVAYH
                     FDDETVSLHITAAPGLLRKSSIDFRPARRPHVYYPRDTGAFFNYGLNYAGGQPSGHRS
                     FTGTTQVGVRAGDLLLLSDSNYTRSGDDGRFVRLATSVTHESRLDLNRTVLCDLYAAS
                     GYLGSAVNMGGISFSRNFSIDPYFVKQPMVDYRGFATLPSEVRVLMDGAQVRSQKIGP
                     GPFDLRNILAFNGVHDLTITVRDAFGREETVRYPFYSTDTLLARGLHEFSYNAGFLRR
                     GFGTESNDYGGFAASFFHNYGLTDSITIGVRGEGLSDLVNAGPRMSWLIRRYGTLGLS
                     AAASLSRDTRAGYATSLGYTYQPRNASLRLLYNRYTREYATISTRTTDDRTRSELGAA
                     VGYGTSRLGSISFDFAAI"
BASE COUNT          813 a         1202 c         1149 g          704 t
ORIGIN      
        1 cgatcgaagc ggagacgacc gtcgaggata tcctcgtcag cgtcgggcgg acgggtgccc
       61 tcacccccgt cgcctctctc aatcccgtca ccgtgggcgg ggtggaggtc tcccgggcca
      121 ccctgcacaa cgaggacgag atagcgcgaa aggacgtgag ggttggggac agggtcatcg
      181 tgacccgcgc gggcgacgtc atccccgagg tcgtcagggt cctcgacgcc gcgaggccgg
      241 gccggacgga acctttcagg atgcccgaaa agtgccccgt ctgcggcgaa gccgtcgtga
      301 gaccccccgg cgaggccata cggcgctgcg taaacatcag ctgccccgct cagatcaagg
      361 ggagcatcga gcacttcgcc tcgaagcggg ccatggacat cgacggcctg ggcacgaagc
      421 tcgtggaaca actcgtcgac aggcaggtca tccgcgatgt gtcggacctc tattatataa
      481 ggaaggaaac ccttgccggc ctggatcgga tggccgacaa gtccgccggc aacatcatcg
      541 acgccatcga ggcgtcgaaa cacagaccct ttgcccggtt cctcaacgcg ctgggcatcc
      601 ggcaggtggg cgaacacgcg gcggacctcc tggcggagcg tttcgattcc atcgagaagc
      661 tcatggcggc ggacacccag atgctgctgg ccatacccga gatcggcccc gaggcggcgg
      721 gcagcatcac cgcttttttc cgcgacgcga agaaccgcga gaccataaag aggatcctcg
      781 acgccggcgt caagctcgag tacgaacagg gcggcgtgaa gaagctcgcg ggcctcacct
      841 tcgtcttcac gggctccctc tcatccatgt cccgcgacga ggcccgggca aaggtcgagg
      901 atctcggcgc gaagaccgcc tcgtcggtga gcaagaaggt cagctacgtc gtcgccggcg
      961 aagaggccgg ctccaagctc gacaaggccc ggcagctcgg cctcaccata ctcacggaag
     1021 aggagttcct cgctcttctc acgtagtcac tccaaaaacc ctccatatcc ttacgattcg
     1081 tcatttttca ggtccgattg aacacagcgg cctatcttta caaccccgct tgtgttgaaa
     1141 aatacacata aatcaaaagc aaggaggaca caatgaaacg cgtcatcagt ctttcagggt
     1201 tgttttgcct ggcggtgctc attgtcgcaa cgggcttgag tgcggacgct gtcgccggaa
     1261 cgaaaaccgg gaacctggag gtgaccgcca cctgttccgc aagctgtgag ttcctgacac
     1321 aggggacaaa cctcgcattc ggaccgcacg acccgctcgg cggcacggaa accaccgccc
     1381 aggcgtcgtg gagttacagg tgcgtggcag gtacaaaata caagctgtac atagatggcg
     1441 agcggcagat gctgctcgat ggcacggact cgggcaacaa acttgcattc cagctctaca
     1501 aggacgacta ctcaacggtc tttcccgaat cggagtcgac cgccggagaa ctgacggccg
     1561 agtcgaacgc ggagaccccc ttcacgatca acggcaagat agcagcggga gacggccagt
     1621 ccctgaaagc aggctcttac agcaagaccc tgacagtaac actgctctac taggaacaat
     1681 atgtcgagga cagataccct gacgtggccg gtccggaaga ccggccatcg tcctgttctg
     1741 cctttcattc tactggccgc cattgtcctt ctctcctgtg ccccggcatc tgcgcaaagc
     1801 ttcagtgtgc atcccacaag ggtatccttc gacaggacca cgaaggcgca aaagctgatc
     1861 attcggaaca agggcgcaac cgacctttcc ctccaggtgg gagctttcaa gtggtcccag
     1921 aacaaggacg gcagggacgt gtacgaggat acctccgaca tcgtggtctt ccccccgatc
     1981 ttcaagatca ggaagggcga acagaggctg gtgcgcctgg ggacgaccct tggccccacg
     2041 tcgcgggaag gtgcctacag ggtcttcgta gaggagctgc ccgtggcaga cccgaaaaga
     2101 tcgggcccag ggataaatct tgtgttcaag gtggggatac ctctattcgt cagccccctt
     2161 caggcctccc gggaggcgct cggcatctgt ctggcgatcg aagagggaac ggtcgtcctc
     2221 agcgctctca acaacgacaa tgcccacgct gagatagaat cggtaagcat catcggtcag
     2281 gattcccggg agaaagagat attctcgaag ggcttcaatg gctggtacat actcagcggc
     2341 tcatccaggg tctacaaggt cgatattcca ccggcgatct gcaagagact tgcaagcgtc
     2401 catgcggttg tcagaacggc gcggaattcc ttcgaggatg tggtggaggt gacagggggc
     2461 atgtgtgggc cgcagaccag atgatcctgc cgtggctccg cgcgggagcg gcactggttg
     2521 cgctcctcat ccccttgttc ctcgcggtcc ccgcaccgtg cggggaccgg cagcatgtcg
     2581 tactcgaagt catcctcaac acggacaaca agggcgagtt cttcgtctat ctcacggctg
     2641 acggggactg cctcatgagc agggatgatc tcgtcgccat cggctttgac ggccccgccg
     2701 gcacgatatt gcagacagga ggaaggacgt tcatctccct gagatcgatg aaggatgtgg
     2761 cgtatcattt cgacgatgaa acggtgtcgc tccatatcac ggccgcacca ggtctcctga
     2821 ggaaaagctc catcgacttc aggcccgcaa ggcggcccca cgtctattac ccgcgggaca
     2881 ccggggcctt tttcaactac ggcctgaact atgccggcgg gcagccttcc ggccacaggt
     2941 cttttacagg aacgacccag gtcggtgtac gcgcgggtga cctcctcctc ctcagcgatt
     3001 cgaactacac caggagcggt gacgacggga ggtttgtccg gctggcgacc agcgtgaccc
     3061 atgaatcacg gctcgatctc aatcgcaccg tgctctgcga cctctatgcc gcctccggtt
     3121 acctcggcag cgccgttaac atggggggca tcagcttctc ccggaacttc agcatcgatc
     3181 cctatttcgt aaagcagccg atggtggatt acaggggatt cgcgacgttg ccctcggagg
     3241 tacgggtctt gatggacggc gcccaggtcc ggtcgcaaaa gataggtccc ggccccttcg
     3301 acctcaggaa catcctggcc ttcaacggag tccacgatct cacgatcacg gtcagggacg
     3361 ccttcggcag ggaggaaact gttcgctatc ccttctactc cacggacact ctgctggccc
     3421 gcgggcttca cgagttcagc tacaacgccg gcttcttgag gaggggcttc gggacggaaa
     3481 gcaacgatta tggggggttc gccgcgtctt tcttccacaa ctacggcctg accgactcga
     3541 tcaccatcgg cgtaagaggc gagggcctga gcgatctcgt caacgcgggc ccgagaatgt
     3601 cctggcttat caggcgctac gggaccctgg gcctcagcgc ggccgcgagc ctctcacggg
     3661 acacacgggc cggctatgca acctccctgg gctataccta tcagcctcgc aacgcgagcc
     3721 tgaggctcct ctacaacagg tacaccaggg aatacgctac gatctccaca cggaccactg
     3781 acgacaggac acggtcggaa ctgggcgctg ctgttggcta cggtacctcc cgtcttggtt
     3841 cgatctcatt cgacttcgcg gccatcag
//
LOCUS       DAOQTR010000166         3867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26555_length_3867_cov_2.969045, whole genome shotgun sequence.
ACCESSION   DAOQTR010000166 DAOQTR010000000
VERSION     DAOQTR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26555_length_3867_cov_2.969045"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PKN85_05135"
     CDS             complement(<1..1445)
                     /locus_tag="PKN85_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43822.1"
                     /translation="MNSRKSSSTLRSIMRLLCGGSFLISRDLRIEWVGSVSRRWSGGK
                     DAVTLEGKHCYSVIMGRRKPCESCPVARCYESGRMEEMEIKIGKGADAEHFVLTASPL
                     SSEDGGLMGVVEIIRDVTAFRRTEEDLRQLGEFNYEIVENAPVAIFTINKKGEFSSVN
                     PALVTLSGFDSMAKAKEKLLGFNWIKNPYTKKCGMANHIREGLRGKSFQLWDFPFINY
                     WGVPQYIYFKGVPIKDKDGRVEGLLCIIEETTERVRASEQLMQEAKMSMIGRLTTGIA
                     HELNNPLATITANAELALESLENLEGGTFTRKDMEEFKESLRVIEEQAFRCTKTIKSL
                     LDVTRKKDLGKTSIDLTGLLDEILDHINFQRLPVRFIEDVPSPLPAVKGDFDAMRQVF
                     LNVIVNAVDAVEGQKDAAIWIKVRRMPGNTLEVAIEDNGPGIPEKIRELIFEPFFTTK
                     GIKKGTGLGLTLCYDFLQRMGGGIEVGQRLEG"
     gene            1837..2241
                     /locus_tag="PKN85_05140"
     CDS             1837..2241
                     /locus_tag="PKN85_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HNT43823.1"
                     /translation="MFQNTMGGGMNLGFPDVCLTPTPVGPIPIPYPNISTGATTNPAT
                     AALTVLCDGMPALNQMSMGTISNGDNAGVNLGVASGLVMGPTEFILGSFTVLKGGTPA
                     QRMTSITGHNGLSMNGPGVSLAPSQVAVLVLG"
     gene            2242..>3867
                     /locus_tag="PKN85_05145"
     CDS             2242..>3867
                     /locus_tag="PKN85_05145"
                     /inference="COORDINATES: protein motif:HMM:NF022839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-transferase family protein"
                     /protein_id="HNT43824.1"
                     /translation="MESIFISIASYRDNELGRTIRDCTDKAAHPERLVFGIHWQHDNH
                     EFFEQFYDRRCRVIDTDYRESRGACWARHEAQKLYAGEDFLLQIDSHMRFVRDWDRIC
                     VDGVRKLQERGNRRVIISNLMPSYDPDDDGNLPLAYLTYRMGSFNQDGAIALKASVVQ
                     KPPPEPFSPGFSISAAFVFSIGDLYREVPIDPDLYFDGEEISYAVRAYTHGYDIYHCH
                     HPIVYHYYTRKTDRKHWDDHGNWGERSLMAKARMRTLLMRTGEVADLGRYGLGTMRSI
                     EEYEGHSGVNFRERTVKSPAQEESFPVTRDWIRSKLAGGGIGTAQIDPFSYCNARCWY
                     CPVRYHPQPLEARRHMPIALFERIVEDLCEERDKKGLVSPGFNFIYTAHYNEVLLYRH
                     LTDMFEVLRKHGLRTLVLTNGIALTPQKADLIARYDDVVGGQVCVNISAFERDRWIAN
                     TIAGNGASPVTMRRTFNRTMKNLEYASQRLRGVSIQVNEPDPNQALRQADLARRMFPG
                     ATVRSVTSLSDRAGILHEAGVLSNREEIARRSAG"
BASE COUNT          765 a         1243 c         1074 g          785 t
ORIGIN      
        1 ccctcgaggc gctgccccac ctcgatgccg ccccccatcc tctgcaggaa atcataacat
       61 agcgtgagcc ccagtcccgt ccccttcttt atccccttcg tcgtgaaaaa gggctcgaag
      121 ataagttcgc gaatcttctc ggggatgccc gggccgttgt cctctatggc aacctcgagg
      181 gtgtttccgg gcatccgccg gacctttatc catatggcgg cgtccttctg cccctcgacg
      241 gcgtcgacgg cattgacgat gacgttgaga aagacctgcc gcatggcgtc gaaatccccc
      301 ttcaccgccg gcaggggaga gggaacatcc tcgatgaacc tcaccggcag cctctggaaa
      361 ttgatgtgat cgaggatctc gtcgaggagc ccggtgagat cgatgctcgt cttccccagg
      421 tcctttttcc tcgtcacgtc gaggaggctc ttgatggtct tggtgcacct gaaggcctgc
      481 tcctctatca ccctcagact ctccttgaac tcctccatgt ccttcctggt gaaggtgccg
      541 ccctcgaggt tctccaggga ttccagggca agctcggcgt tggccgttat ggttgccagc
      601 gggttgttga gctcatgggc aatgcccgtc gtgagacgcc ctatcatgga catcttggct
      661 tcctgcatga gctgttcgct ggccctgacg cgctccgtcg tctcctcgat gatgcacagg
      721 agcccctcca cccggccgtc cttgtccttg atgggaacac ccttgaagta gatgtactgc
      781 ggcacccccc agtaattgat gaaggggaaa tcccagagct gaaaggactt tcccctgagg
      841 ccttccctga tgtggttcgc catgccgcat ttcttcgtgt aggggttctt tatccaattg
      901 aacccgagga gcttctcttt cgccttcgcc atgctgtcga accccgacag ggtgacgagc
      961 gccgggttga cgcttgaaaa ctcccccttc ttattaatgg taaagatcgc caccggggcg
     1021 ttctccacga tctcataatt gaactccccg agctggcgaa ggtcctcctc ggtcctccgg
     1081 aaggccgtga cgtcccgtat gatctcgaca acccccatga gaccgccgtc ctcgctgctc
     1141 aagggggacg cggtgaggac aaagtgttcg gcgtcggccc ccttgccgat ctttatctcc
     1201 atctcctcca tcctgccgct ttcgtaacac ctcgcgacgg gacagctctc gcagggcttg
     1261 cgcctcccca tgatgacgga atagcagtgc ttgccttcaa gcgttacggc atccttccct
     1321 cccgaccacc ggcggctcac ggaccccacc cattcgatcc tgaggtcccg gctgatcagg
     1381 aaggaccccc cgcacagaag gcgcatgatc gacctcaggg tggaggagct cttccgtgaa
     1441 ttcatctcgg ccatgacgag ataggatagc aaaaaaggtg ccagacgtaa aggtcgcagc
     1501 cgggggctgc gaccgcttga gtggcttgaa tcgcttgaat ggcttgagag ttagaggacg
     1561 gagcggaccg aaaggccttg cgtccacact ccagcctggg tgtaccggca agcctgattc
     1621 ttctactttc aagccactca agcgattcaa gccactcaag cggtctttac ggtctttacc
     1681 tgtctttacc tgttttccct tgactttatc accacatccc catatcatct aaacgaaaat
     1741 gtgcgctatg tcccttatct tattgagata gttcctaccc ggtccgggtc tgttgttgac
     1801 tgttccgtcg gcacatctgg cctgaaagga gggactatgt ttcagaatac catggggggc
     1861 gggatgaacc tgggctttcc cgacgtatgt ctcacgccga caccggtggg gccgataccc
     1921 atcccctatc ccaatatctc aacgggagca acgacaaacc cggcaacagc cgcgctcacc
     1981 gtgctatgtg acggcatgcc cgcgctcaac cagatgtcga tggggacgat aagcaacggg
     2041 gacaatgccg gtgtgaacct cggagtcgct tcggggctcg tcatgggacc gacggagttc
     2101 atcctcggga gtttcaccgt cctgaaagga gggacaccgg cgcagaggat gacaagcatt
     2161 acggggcata atggcctgtc catgaacggg cccggcgtca gtctcgcccc gagccaggtc
     2221 gcggtgcttg tcctggggta gatggaaagc atcttcatca gcatcgccag ctaccgggac
     2281 aacgagttgg gcaggacgat acgcgattgc accgacaagg cggcccaccc cgaacggctc
     2341 gtgttcggca ttcactggca gcacgacaac cacgagttct tcgagcagtt ctatgaccgc
     2401 cgctgcaggg ttatcgacac ggactacagg gaatcgcggg gggcctgctg ggcgcgccat
     2461 gaggcgcaga agctctatgc cggagaggat ttcctcctcc agatcgacag ccacatgcgg
     2521 ttcgtgcgcg attgggacag gatctgcgtc gacggtgtaa ggaaactcca ggaaaggggg
     2581 aacaggcggg tcatcatcag caaccttatg ccctcctacg accccgatga cgacgggaac
     2641 ctccccctgg cctaccttac gtacaggatg ggctccttca accaggatgg cgccatcgcg
     2701 ctcaaggcgt ccgtcgtcca aaaaccaccg cccgagcctt tcagccccgg tttctccatc
     2761 tccgccgcct ttgtcttctc catcggcgac ctctaccggg aggtccccat cgaccccgac
     2821 ctctatttcg acggggagga gatctcctat gcggtgaggg cctacaccca tggctacgat
     2881 atctatcact gccaccaccc catcgtctac cattattata caaggaagac ggaccgcaag
     2941 cactgggacg accacggcaa ctggggggaa aggtccttga tggcaaaggc ccggatgcgg
     3001 acccttctca tgcggaccgg tgaggtcgcc gacctcggac gttacgggct gggcaccatg
     3061 cgctccatcg aggagtacga ggggcattcc ggcgtcaatt tccgggagcg caccgtgaaa
     3121 agccccgctc aagaggagtc cttccctgtc accagggact ggatacgctc gaagctggcc
     3181 gggggaggca tcggcaccgc ccagatcgac cccttctcct actgcaacgc ccggtgctgg
     3241 tactgccccg tgcgctatca cccgcaaccc ttggaggcac gacggcacat gcccatcgcc
     3301 ctcttcgaga ggatcgtgga ggacctctgc gaggagaggg acaaaaaggg tcttgtcagc
     3361 cccggcttta attttatcta taccgcccac tacaacgagg ttctccttta caggcacctc
     3421 accgatatgt tcgaggtcct gcgcaagcac gggctcagga ccctcgtcct cacaaacggt
     3481 atcgccctta cccctcagaa ggcggacctc atcgcccgtt atgacgatgt ggtcggaggg
     3541 caggtctgcg tcaacatcag cgcattcgaa cgggaccggt ggatcgcgaa cacgatcgcc
     3601 ggcaacggcg cctctcccgt cacgatgcgc cggaccttca accggacgat gaagaacctc
     3661 gaatacgcct ctcaacgcct ccggggcgtt tcgatccagg tgaacgagcc agaccccaac
     3721 caggcactaa ggcaggccga cctcgcgcgc cgcatgttcc ccggtgccac cgtccgttcc
     3781 gtcacgagcc tctccgaccg ggccggcata ctccacgagg ccggtgtcct gtcaaatcgg
     3841 gaggagatcg cccgaagaag cgccggc
//
LOCUS       DAOQTR010000167         3864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26582_length_3864_cov_3.661066, whole genome shotgun sequence.
ACCESSION   DAOQTR010000167 DAOQTR010000000
VERSION     DAOQTR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26582_length_3864_cov_3.661066"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKN85_05150"
     CDS             <1..223
                     /locus_tag="PKN85_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNT43825.1"
                     /translation="KKAPGIKVVDDPGKNKYPLAIDAAGKDDTYVGRIRVDDTVKYGL
                     NMWIVADNIRKGAALNAVQIAEALIKKYL"
     gene            complement(307..3168)
                     /locus_tag="PKN85_05155"
     CDS             complement(307..3168)
                     /locus_tag="PKN85_05155"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNT43826.1"
                     /translation="MFGFLRDLKLNIKISILGGGSVLITAAALVALAVWQSGQYNELA
                     QREVDRLLDADLDHIAQGAYNLVRTEDEAVKEQLSRNLNVMRHILTARGGADLSRQTV
                     EWTVVNQFTGQSVKTRLPRMLVGGKWIGQNSQPAVRTPVVDEVESLVGETATIFQRVN
                     ERGDMLRVATNVRDSEGRRAIGTYIPAVDDDGSPNPVIAAVLQGKTYHGRAFVVNAWY
                     LTAYEPLNDGAGNLVGMIYVGIKQKSVEMRIRHAIQQIKVGKTGYVYVLGGKGEDRGR
                     YIISWKGERDDENVWESTDSDGRYVIQEIIKKATVLKPGEMATERYRWQNPGETEPRW
                     KVARLAYYAPWDWVIGVGVHDDELHSYRAILSGGRNRMVRIMGVAGITITLFIGLLGI
                     FIAWTITRHIRQMTKAAEVIIAGDLNQTVDAASRDEVGTLARTFNLMTERLRETVAGL
                     RRSEEKYRSIFENSVEGIFRSTPQGRFLSINPAGVRMFGYGSQQEMIDSVVDIAQELY
                     VDPGDRERRKELLGSRGIVEGFEVEMRRKDGTRIWVSINSRVVRDAAGQVLYYDNTAE
                     DITERRRLEAQVRQSQKMEAIGTLAGGIAHDFNNILSVLMGYASLLQIELNEDDPLQE
                     YVEHIMASSQKAVNLTSSLLTFSRLQPVHLCPVDLNEAIRGTQKLLERLITDDIELKA
                     SLSTEDLVVMADTTQIDQILFNLASNARDAMPKGGTLTIETELVELDSDLVAAHGLGE
                     PGKYALLSVSDNGSGMDEHVKEHIFDPFFSTKETGKGTGLGLSTVYGIVKRHGGHINV
                     YSEKGMGTTFRIYLPAISGIAEQEIVSGQQVRGGTETVLVADDDADVRRLIRDVLTKY
                     GYRVLEAVDGEDAVEKFRQAGKVDLLVIDSVMPGKNGREAYEAISRITPGMKVIFISG
                     YTQDVVLEKGIEEGEFDFLSKPLGSNMLLRMVREVLDRE"
     gene            3352..>3864
                     /locus_tag="PKN85_05160"
     CDS             3352..>3864
                     /locus_tag="PKN85_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43827.1"
                     /translation="MPDIFAADGSGRELTFQVPLSEIYKFNEPEKAKELGQTLETGPQ
                     RPAAMAPAKRFIISTIGIFCLMIGLTGIFGQNPNIFSFLWIVLGGAIIWVFLAKPEMD
                     KKKAGTAPDTKKDPEVTLVVNAGRIVTRSPHNETARDWTEFAEYKKTKKGVRLSFLDG
                     TNIWLPENTFY"
BASE COUNT          754 a         1252 c         1009 g          849 t
ORIGIN      
        1 gaaaaaggcc cctggtatca aagtggtcga cgacccgggc aagaacaagt atcccctcgc
       61 catcgacgcc gccggcaagg acgacaccta cgtcggcagg atcagggttg acgataccgt
      121 caagtacggc ctcaacatgt ggatagtcgc cgacaacatc aggaaaggtg ccgccctgaa
      181 cgccgtccag atagcggaag ccctgataaa gaaatatctg taaccttcag accggattgc
      241 gaaaaccctc cctcgaaagg ggagggtttt ttctttcacg ccgggccttg aacgcgggag
      301 acctcgtcat tccctgtcca gcacctccct gaccatcctg aggagcatgt tggaccccag
      361 gggcttcgag aggaaatcga actctccctc ctcaatgcct ttctccagga ccacatcctg
      421 ggtgtacccg cttatgaaga tgaccttcat accgggagtg atcctgctta tcgcctcgta
      481 ggcttccctc ccgttctttc cgggcatcac cgagtcgatg accagaaggt ccaccttccc
      541 ggcttgcctg aacttttcca ccgcgtcttc accgtcgacg gcttcgagga ccctataccc
      601 gtacttcgtc aacacgtccc gtatcagacg ccgcacgtcg gcatcgtcgt cggcaacgag
      661 aacggtttcg gtgccccctc tcacctgctg cccggacacg atctcctgtt ccgcgatccc
      721 ggagatcgcg ggaaggtata tgcggaaggt cgtgcccatg cccttctcgc tgtacacatt
      781 gatatgacct ccatgccgct ttacgatccc gtagaccgtc gagagtccca gaccggttcc
      841 tttgcctgtc tccttggtag agaagaaggg atcgaaaatg tgctctttca cgtgttcgtc
      901 catgccggat ccgttgtcgg agacggacag gagggcgtat ttccccggtt cacccagtcc
      961 gtgagcggca acgagatcgc tgtccagttc cacgagttcc gtctcgatcg tcagcgtccc
     1021 gcctttcggc atggcgtccc tggcattgct cgcaaggttg aagagtatct gatcgatctg
     1081 ggtggtgtcc gccatgacga ccaggtcttc cgtgctgaga gatgccttga gctcgatatc
     1141 gtcggtaatg agcctttcca gcaatttctg ggtgcccctt atcgcctcgt tcaggtcgac
     1201 aggacagagg tgtaccggct ggagcctgct gaaggtaagc agactcgatg tcaggttcac
     1261 ggccttctgt gaagacgcca tgatgtgctc aacgtattcc tgaagggggt cgtcctcgtt
     1321 gagctcgatc tgcaggagcg acgcgtaccc catcaggacc gaaagaatgt tgttgaaatc
     1381 atgggcgata ccgcccgcga gggtgccaat ggcctccatc ttctgcgact gccggacctg
     1441 ggcctcgaga cgcctgcgct cggttatgtc ctcggcggta ttatcgtagt agagcacctg
     1501 ccccgcggca tcacggacca ctctggagtt tatgctcacc cagatcctgg tgccgtcctt
     1561 gcggcgcatt tccacctcga aaccttccac gataccccgc gacccaagca gctcttttct
     1621 tctttcccgg tccccgggat cgacatacaa ctcctgagct atgtcaacga cggaatctat
     1681 catctcctgc tgcgatccgt acccgaacat ccgcacaccc gccggattga tgctcaggaa
     1741 ccgcccctga ggtgtcgacc ggaatatccc ctccacggag ttctcgaaga tgctccggta
     1801 tttctcctcg ctccgacgca gtcccgccac tgtttccctg agcctttccg tcatcaagtt
     1861 gaatgtccgc gccagggtgc cgacctcgtc gcgggaggcg gcatccactg tctggttgag
     1921 atcgccggca ataatgacct cagcggcttt cgtcatctga cggatgtggc gagtgatggt
     1981 ccaggcgatg aagatgccca ggagtcctat gaacagtgtg atcgtgatcc cggccacacc
     2041 catgatgcgc accatccggt tccttccgcc gctcaggatt gcacgatacg agtgcagctc
     2101 atcgtcgtga acacctaccc ctatcaccca gtcccaggga gcgtaatagg cgaggcgggc
     2161 caccttccac ctcggctccg tctcccccgg gttctgccag cggtaacgtt ccgtggccat
     2221 ttccccgggt ttcagaaccg ttgccttctt tatgatctcc tggatcacgt agcggccgtc
     2281 actgtccgtg ctctcccaga cgttttcgtc gtccctctcc cccttccagg atatgatgta
     2341 gcggccccgg tcctctccct tgcctcccag gacgtagaca tatcccgtct ttccgacctt
     2401 gatctgctgg atcgcatggc gtatccgcat ttcgacgctc ttctgtttga taccgacata
     2461 tatcatcccc accaggttgc ccgccccgtc gttgagcggt tcgtacgccg tcaggtacca
     2521 ggcgttcacc acaaaggcac ggccgtgata cgtctttccc tgcaacaccg ccgcgatgac
     2581 agggttcggg ctgccgtcgt catcgacagc gggtatgtag gttcctatgg cacgcctgcc
     2641 ctctgaatcc ctgacgttcg tcgccacgcg gagcatgtct ccccgctcgt tcacgcgctg
     2701 aaagatggtg gcagtctcac cgacaaggct ctcgacctcg tcgaccaccg gggttctgac
     2761 agcgggctgc gaattctgcc cgatccattt cccgccaacg agcatccgcg gcaatctcgt
     2821 cttcacggac tgtccggtga actggttcac cactgtccac tccacggtct gacgggagag
     2881 gtccgcgccg cccctggcgg tcagtatgtg gcgcatgaca ttgaggtttc ttgacagctg
     2941 ctccttgacg gcctcatcct ccgtccggac caggttgtag gcaccctggg cgatgtggtc
     3001 caggtccgca tccagcagcc ggtccacctc tctctgcgcg agctcgttgt attgaccgct
     3061 ctgccagacg gcgagcgcca caagggcagc cgccgtgatg aggacgcttc ctcctcccag
     3121 gatggatatc ttgatgttga gcttcaggtc cctaagaaaa ccaaacatcc gctccttcct
     3181 aaccctcaca accccccatt aggcaagtat agcaccgagg ccttcttttg ggtgttcctt
     3241 atgcggacat cgaaactgtc gccaggaaaa cccctttctt cccatcaccc atcacccgct
     3301 tttcttgctt ttccctttgc atttgaagcc acaaacgcct agaatatccc catgccagat
     3361 atctttgcag cggacggatc cgggcgggaa ctgacattcc aggtccctct atccgagata
     3421 tacaagttca acgaaccgga aaaggccaag gagcttggcc agacactgga gaccgggccg
     3481 cagaggcctg ccgccatggc tcccgccaag aggttcatca taagcacgat agggatcttc
     3541 tgcctcatga tcggcctaac gggcatcttc ggccagaacc ccaacatttt cagcttcctc
     3601 tggatcgtcc tcggcggggc gatcatctgg gtctttctgg caaagccgga gatggacaag
     3661 aagaaagccg ggaccgcccc ggatacgaag aaggaccccg aggtgaccct ggttgtcaac
     3721 gccggcagga tcgtcacgcg ctcgccccac aacgaaacag cgagggactg gacggaattc
     3781 gctgagtaca agaaaacaaa gaagggggtc cgcctgagct tcctcgacgg cacgaacatc
     3841 tggctccccg aaaacacctt ctat
//
LOCUS       DAOQTR010000168         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26738_length_3848_cov_3.814922, whole genome shotgun sequence.
ACCESSION   DAOQTR010000168 DAOQTR010000000
VERSION     DAOQTR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26738_length_3848_cov_3.814922"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..2132)
                     /locus_tag="PKN85_05165"
     CDS             complement(30..2132)
                     /locus_tag="PKN85_05165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT43828.1"
                     /translation="MPKKKRRVRNIILLASVSFVISLALFLFDVLATYENRSFDLFSR
                     YLNPERASDRIVIIEIDQQSLDALSAESINWPWPRQVYAPIIEFASLADAFLVDILFT
                     EPSSYGQEDDMMLGDAIAKASNVYLPFFLSMNEKAMAPEDIAYLKRFAVPGGPPPKPG
                     YRSVVTPIDAIRHGVKGSGNVAISPDRDGVYRRVPLVFTLAEMTLPSLTFSWFTGRGD
                     TKMDSRGLLLRDRRLPLTEGRLLLRFPSGGKPFTVIPAIDVLSAYRGADPNGRAMSRD
                     FFKGKVVFLGMTAPGLLDLKSTPTMPVSTGVHINATLFENLYLSTFMRPVPVLFVVFS
                     MLLVCVFIAAFVLTFHTIAKNLSVFAAGLVLILAVAAALFSGRYYLPTTYLVVTLLMS
                     FILSAIFSYALEGRERQFIKRTFSQYMDKTIVEHVLQNPDIVRPGGKKARVTVLFADI
                     AGFTTISERNSPEDTALMLHRVLNELTEVVIDEKGVVDKYIGDCIMAFWGSPLETPKD
                     EINACRSSLRFIDAIGGINGVFEREGIPPVAIRVGLHTGDAIAGNMGSVRLFDFTVVG
                     DTVNLASRLESVNKVFGTNIIASEHTVAKTYDLFLSRSLGPIEVKGKSEPVVVHEVLC
                     EAGDAPAALRRKVDLYNEAFQTYREQRFTEAVERFDVLLKEFPHDGPSEFYRRRAESL
                     AGNFSLTKEWDIIRMTEK"
     gene            2329..3084
                     /locus_tag="PKN85_05170"
     CDS             2329..3084
                     /locus_tag="PKN85_05170"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT43829.1"
                     /translation="MRLKNKVALLTAAAGAGIGQATARVMAREGAAVVVTDIHDDRAR
                     TVAEQIAGEYGARTLGLKCDVTSTSDVNRVVEVTLQAFGRVDILFNNAGTNRPTRVVD
                     ITDEVWDLVLATTLTGTFHCCRAVVPAMIRQNGGRIISVTSVAGFRGLAGGHAHYAAA
                     KAGVMAFTRCLAMEVAPHRITANTIAPSFIFNEFIPHIYPQDEIDRMFDEIPYPRKGT
                     PEDVANAALFLASDEGEYMTGQTLCVTGGSWMR"
     gene            3290..>3848
                     /locus_tag="PKN85_05175"
     CDS             3290..>3848
                     /locus_tag="PKN85_05175"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT43830.1"
                     /translation="MITKLKPDELYKKCDPDSFAFAGTDAVVELPQTIGQEKALNSLD
                     FGLNMDSAGFNIFAIGESGTGKMSTVMYMLRKKAANEEAPADWCYVHNFKDPDSSIAL
                     SFGPGRGAVFQKELEEMIKILKLEIPKAFESKEYETQKSKIVDEFQQKQQEYLARLDS
                     EAKERGFTVKRGPTGVLIVPVKENGE"
BASE COUNT          851 a         1058 c         1170 g          769 t
ORIGIN      
        1 tatcaatgat aggaatatgg ctttcttcat catttttcgg tcattctaat aatatcccat
       61 tcttttgtca aagaaaaatt ccccgccagg gattcggccc gtctcctgta gaattcgctc
      121 ggtccgtcgt gggggaattc cttgagaaga acgtcaaaac gctccacggc ctcggtgaat
      181 cgctgctccc tgtaggtctg gaatgcctcg ttataaaggt cgacctttct tctgagtgcg
      241 gcgggtgcgt cacctgcctc gcagaggacc tcgtggacca cgacgggctc cgatttcccc
      301 ttcacctcga tgggacccag gctccgcgac aggaagagat cgtaagtctt cgcgacggtg
      361 tgttcgctgg caatgatgtt cgtgccgaag accttgttga ccgattcgag gcgtgatgcc
      421 aggttcaccg tgtcgccgac gacggtgaag tcgaaaagcc gcacgcttcc catgttcccc
      481 gcgatggcgt ctcccgtgtg aagacccacc cgtatggcga cgggagggat gccctcccgc
      541 tcgaagaccc cgtttatccc gccgatagcg tcgatgaacc tgagcgagct ccggcaggcg
      601 ttgatctcgt ccttcggtgt ttcgagaggc gaaccccaga aggccatgat gcagtcaccg
      661 atgtacttgt ccaccacccc tttctcatcg atgacgacct cggtgagttc gttcaggacc
      721 cggtggagca tgagcgccgt gtcctccggg gaattcctct ccgagatggt ggtaaacccg
      781 gcgatgtcgg cgaagagcac cgtgaccctg gccttcttgc cgccgggcct gacgatgtcc
      841 gggttctgga ggacgtgctc cacgatggtc ttgtccatgt actgcgaaaa ggtcctcttg
      901 atgaactgcc tttcccgccc ctcgagggcg tagctgaaga tggcggagag aatgaagctc
      961 ataaggaggg tcaccacgag ataggtcgtc gggaggtagt accggccgga gaagagggcc
     1021 gccgccacgg ccaggatgag aacgaggccc gccgcgaaga cggagaggtt cttcgctatg
     1081 gtgtgaaaag tgagtacgaa ggccgcgatg aagacgcaga cgaggagcat tgaaaagacg
     1141 acaaagagca ccggaacggg gcgcatgaag gtactcaggt agaggttctc aaaaagcgtg
     1201 gcatttatgt ggacacccgt cgagacaggc attgtcggcg ttgacttcag gtccagaagg
     1261 ccgggtgccg tcatccccag gaagacgacc ttccccttga agaagtcccg ggacatggcc
     1321 ctgccgtttg ggtctgcacc gcggtacgcg ctcaggacat cgatggcagg gatgaccgta
     1381 aagggtttcc cgccgctggg aaaacgcagg aggagccgcc cctccgtcag aggcaggcgc
     1441 ctgtctcgca gaaggagtcc gcgggagtcc attttcgtgt ccccgcggcc cgtgaaccag
     1501 gagaaggtga gactggggag cgtcatctcc gcgagggtga atacgagagg taccctgcgg
     1561 tacaccccgt ccctgtcggg gctgatggcg acatttcctg aacccttcac gccgtggcgt
     1621 atcgcatcga tgggtgtcac gacggaacga tatccgggtt ttggcggcgg acctccggga
     1681 acggcaaaac gcttgaggta cgcgatatct tccggggcca tggccttctc gttcatcgac
     1741 aggaagaagg gcaggtagac attggacgcc ttcgcgatgg cgtcgcccag catcatgtca
     1801 tcctcctggc cgtaagagga gggttcggtg aaaaggatat ccacgaggaa ggcatcagcc
     1861 agtgaggcaa actcgatgat gggcgcgtag acctgccggg gccagggcca gttaatgctc
     1921 tcggcgctga gcgcgtctaa cgactgctgg tcgatctcga tgatgacgat gcggtccgac
     1981 gcccgttcgg ggttgagata acgggagaag agatcaaagg agcggttctc gtaagtggca
     2041 agaacatcaa agagaaaaag cgcaagagag ataacaaagg atactgaagc gagaagtatg
     2101 atattgcgca cccgacgttt cttcttcggc acccggttct ccctatgtcc taaagtgagc
     2161 attcatacca tatcacatac gtcggaaaaa aataacctgt aaaggttcac tgctgttctt
     2221 ttttggcctt taacccggaa ctcgcaacgc gcaacccgca gccgtcttta tttccttgag
     2281 cctcatcccg ccgattatgt atcattaggt tcaaggagaa cagggaccat gagactcaag
     2341 aacaaagttg cccttcttac tgcggccgcg ggcgccggca ttggacaggc gacggcccgg
     2401 gtgatggcgc gggagggcgc cgcggtggtt gttaccgaca ttcacgacga ccgggccagg
     2461 acggtggcgg agcagatagc cggtgaatac ggggcaagaa cacttggcct caagtgcgac
     2521 gtgacaagca cctccgacgt gaaccgcgtc gtggaggtga cactgcaagc ctttggccgt
     2581 gtcgacatac tcttcaacaa cgcgggaacg aaccgcccca cccgtgtcgt ggacatcacg
     2641 gacgaggtct gggacctcgt gctcgcgacg accctcacgg gtacgttcca ttgttgcagg
     2701 gccgtcgtgc cggcgatgat caggcagaac ggaggccgca ttataagcgt gacctccgtg
     2761 gccggtttcc gcgggcttgc cggtggtcat gcccattacg cggcggcgaa ggcgggtgtc
     2821 atggccttca cgagatgcct tgccatggag gtcgcccccc accgcatcac ggccaacacc
     2881 atagcgccca gtttcatctt caatgagttc atcccgcaca tctatccaca ggatgagatc
     2941 gacaggatgt tcgacgagat accctacccc cgcaagggga ccccggaaga cgtcgcgaac
     3001 gccgcgctct tccttgcctc cgacgaggga gaatacatga cgggccagac cctgtgcgtc
     3061 accggcggaa gctggatgag gtagaaaacg gttcaagggt tcaaacgttc aaaggttcaa
     3121 ggggctgaag ggcggggtac gcttgttttt cccactcttg aacgcttgaa cttctgaact
     3181 cttgaacgtt ttttggttcg gggttgatta ttcgctgtga ggtgttattt tttatgtaac
     3241 gtttcaggac cggaaccttt gtcgacagta cacgatcagg aggcagcccg tgatcacaaa
     3301 gttgaagccc gatgaactct acaagaagtg cgatcccgat tcctttgcct ttgccgggac
     3361 cgatgccgtc gtcgaattgc cgcagacgat cggccaggaa aaggccctca attccctgga
     3421 tttcggtctc aacatggaca gtgccggttt caacatcttc gccatcggcg agagcggcac
     3481 cgggaagatg tccacggtga tgtacatgct caggaagaag gcggccaacg aagaagcccc
     3541 ggctgactgg tgttacgtcc acaacttcaa ggaccccgac agctccatag ccctttcctt
     3601 cgggccgggc aggggtgccg tgttccagaa ggagctcgag gagatgatca agatcctcaa
     3661 gctcgagata ccgaaggcct ttgaatcaaa ggaatacgag acgcagaaga gcaagatcgt
     3721 cgacgagttc cagcagaaac agcaggagta tttggcccgt ctcgacagcg aggcaaagga
     3781 gaggggcttt acggtaaaac gggggccgac gggggtcctc atcgttcccg tcaaggagaa
     3841 cggggaac
//
LOCUS       DAOQTR010000169         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26799_length_3843_cov_3.849525, whole genome shotgun sequence.
ACCESSION   DAOQTR010000169 DAOQTR010000000
VERSION     DAOQTR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26799_length_3843_cov_3.849525"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKN85_05180"
     CDS             complement(<1..421)
                     /locus_tag="PKN85_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT43831.1"
                     /translation="MEDVRRILVASRSTAECKSAFHYGVALARALVAEVSILHIEYDP
                     FEKIGFRYLGSLGPFQQEYLVRRKEIREEIEGLVRDEETAGMAIRQMIREGEPVGETV
                     RAVIENNIDLLVMAAHEEGRIERMIYGRSNHEIVRRLP"
     gene            complement(504..899)
                     /locus_tag="PKN85_05185"
     CDS             complement(504..899)
                     /locus_tag="PKN85_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HNT43832.1"
                     /translation="MSKLGPLFQMEDGKRERHIPVIDCPDRVAAGKPFAINVTIGNEV
                     KHPNTTEHHIKWVQVFFRPDNDRFSYQIAHCDFAAHGDSEAGSGKGPVVTDHRVSVVV
                     SVNGTGRIYALSLCNIHGLWESGKDVEVV"
     gene            complement(1723..3126)
                     /locus_tag="PKN85_05190"
     CDS             complement(1723..3126)
                     /locus_tag="PKN85_05190"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT43833.1"
                     /translation="MARVLIVDDDENFSALLSIMVKRAGHDAVAVATIKEGMKVLRGA
                     CEVVFLDIHLPDGNGLEILQDIRNTPSSPEIIIITGFEDSGAAELALKSGVWDYIRKP
                     SSIKQMQLALLRALQYRKEKGYQGLPAALKMDGIIGKSPLVGACLNLVAKAARGEANV
                     LITGETGTGKELFALSIHNNGPRSNKSFVVVDCASLPQNLVESTLFGHKKGSFTGADR
                     ARDGLIRQAHGGTLFLDEIGELPLSIQKSFLRVLQMRSFRPIGSDTEITSDFRLISAT
                     NRNLDRMVEEGQFREDLLFRIRTLQIDLPPLREHPEDIRELLVHYVARLCEQYGVGNK
                     GFSPEFLAALEAHDWPGNVREFISTLERAISEAFHQPTLYPKHLPAEIRIRIKRSELD
                     MGSGSEPHRETAEPVPVLRTYREEAIAAAEKEYLERVIKISEGRLREICRLSGLSRPR
                     LYALLKKYNLTRQTVPS"
     gene            complement(3518..>3843)
                     /locus_tag="PKN85_05195"
     CDS             complement(3518..>3843)
                     /locus_tag="PKN85_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43834.1"
                     /translation="TTPVWGTVVHALGNLERGGRLVINAIRKEEIDKEELLALSYPEH
                     LWLEKEIKSVANVASRDVREFLELAASIPIRPEVEIFSLDEANRALLELKERKIRGAK
                     VLRIV"
BASE COUNT          761 a         1192 c          997 g          893 t
ORIGIN      
        1 agggaagtct tctgacgatc tcgtgattgc ttctgccgta tatcattcgt tctatccggc
       61 cttcctcatg ggcggccatg acaaggagat ctatgttgtt ctctatcacc gcccgcacgg
      121 tctccccgac cggttccccc tcccggatca tctgcctgat ggccattccc gccgtttctt
      181 catcacgtac aaggccctct atctcctcgc gtatctcctt gcggcgaacg agatattcct
      241 gctgaaaagg accaaggctg ccaaggtatc gaaatccgat cttctcaaat ggatcgtatt
      301 cgatatgcag tatggagacc tctgccacga gggccctggc cagcgcaacg ccgtaatgaa
      361 acgcgctctt gcactccgcg gtggatctgc ttgccacaag aatacgcctc acgtcctcca
      421 tgcaactcct ccttttcagc ggcccttccg tccacaatac cggcaggaaa atactgtcgc
      481 atccgtcgac cggttgtaga tctctagaca acctccacgt ctttgccgct ttcccacaga
      541 ccgtggatgt tgcacaggct cagggcgtag atccttcccg tgccgttcac cgacacgacc
      601 acgctgaccc tgtgatcggt cacgacaggg cccttgccgg agcccgcctc ggaatcaccg
      661 tgggcggcga aatcgcagtg cgctatctgg taggaaaacc tgtcgttatc gggcctgaag
      721 aagacctgga cccacttgat gtgatgctcc gtcgtgttcg gatgcttgac ctcgttgccg
      781 atcgtcacat tgatggcaaa cggcttgccg gcagctaccc tgtcggggca atcgatcacc
      841 gggatgtgtc tttccctctt gccgtcctcc atctgaaaca acggacccaa tttgctcatc
      901 aaccccgcgc ctcgcgcaaa gctcaccact ccttcagtcg ccttcggtcg cctccattcg
      961 gcagctatac gggccttctt catgttgtcc ccagggatga aaacaggaag tgattcgtcg
     1021 cagccgttag tcgggcgagg acccgcgcaa gatatgccat tggcgatccg gatacgacgc
     1081 gtgtctgtag caggcacgcc agggtgctaa acatccgtgg attcagaaag gcccccttcc
     1141 ggtttacacg atgtccgtca tgaccccttg ttagcggttt tgccgggctc gacgtcggtc
     1201 ccatcggcga ccaatcccgg gcagatgcgt tcggaacttc gccgcgatcc ccgtgagacc
     1261 ccagtctttc ggttctattc aggagtcaaa gctgtcagcc ggtagccccc tctacttcaa
     1321 cgtatgttct agggaggtaa acatgcccgc ctttccgcga gctcactccc tggcaagtca
     1381 ttgccgcgtt cggcaaccct cctacaggta tatcaaaaag catgccacgg tatgaccacg
     1441 cagagaagat cgcgtattag ccttatcttt caaggagtaa caccccaact gcggatctca
     1501 ttacggaaag cttttgtctc gtgctgcaac atgatgtctt gccaaaaagt actgtgtctc
     1561 gccgcgcaag acactcattc aagggttcaa gggaaggacc atggaaggaa gtcggttgtc
     1621 gaccgaccag cgcacgtgaa cagcgcattc aattccagct gacgaaccgc cttttctgag
     1681 ttctgcctta tactcatgaa cttttgaacc cttgaacctt ctttacgatg ggaccgtttg
     1741 tcttgtgaga ttgtatttct tcagaagcgc gtagagacgg gggcgcgata atcccgaaag
     1801 ccggcagatc tccctgagcc tgccttcgga gatcttgatc acccgttcaa gatactcttt
     1861 ctcagcggca gcgatcgctt cctcacggta cgtcctgaga actggaacgg gttccgcggt
     1921 ttcgcgatgg ggttcggagc cggagcccat gtcgagttcg gatcttttga tcctgatccg
     1981 tatctcggca ggcaggtgtt tcgggtagag cgtaggctgg tgaaacgcct ccgaaatagc
     2041 cctttccaac gtgctgatga actccctgac gttacccggc cagtcatgcg cctcgagggc
     2101 cgcaaggaat tccggtgaaa aacccttgtt gcccaccccg tattgctcac acagacgggc
     2161 gacgtagtgc acgagaagtt cccttatatc ttcggggtgc tcgcgcagcg gcggcagatc
     2221 gatctgcagt gtacgtatcc gaaaaaggag gtcctcccgg aactgcccct cttcgaccat
     2281 cctgtccaaa ttcctgttcg tggccgagat cagcctgaag tcgctcgtga tctccgtgtc
     2341 ggagcctatg gggcgaaagc tccgcatctg caggaccctg aggaaactct tctggatgga
     2401 aaggggaagc tccccgatct catcaagaaa cagcgtgccc ccgtgggcct gtctgatgag
     2461 gccatcccgc gctctatccg ctcccgtgaa ggagcccttt ttgtgaccga agagcgtgct
     2521 ttccacgagg ttttgcggaa gagacgcgca atccacaacg acaaagctct tattggaccg
     2581 ggggccgttg ttgtggatgg ataaggcaaa aagttccttc cccgtgcccg tttcaccggt
     2641 gatgaggacg ttcgcctcac cccgggccgc cttggcgaca aggttcagac aggcaccgac
     2701 cagcgggctc ttcccgatga taccgtccat tttgagggcg gcgggaagac cctggtatcc
     2761 cttttccttc ctgtactgca gggccctgag cagcgcgagc tgcatttgct tgatggacga
     2821 cggttttctt atgtaatccc ataccccgct cttcagggca agctcagcag ctcctgaatc
     2881 ttcgaaaccc gttataatga tgatctccgg tgatgagggg gtgttcctga tgtcctgcag
     2941 gatctcgagg ccgttcccgt cgggtagatg aatatcgaga aagaccacct cgcaggctcc
     3001 cctgaggacc ttcatgcctt cctttatggt cgccaccgcc acggcatcgt gcccggcccg
     3061 cttgaccatg atggaaagca gcgcggagaa gttctcgtca tcgtctacga tcagaacccg
     3121 agccatcgcc cgtcgcctcc cggtgaagca ggcttctgcc gcaagcctta ccggttcacg
     3181 aatgtgatgc ttcgtctgac gcagacctgt gatctacagc agaactcccg acatgagtcc
     3241 gagcgggaac tgccatcctt ccccttatct ctccgtattc atcgagaggc cggcgttcat
     3301 ccgtggccgg cctttcaggg gtcttccgtc tgcgtccccc ggtaacctgc agcacccctt
     3361 acgccgaaaa ctgcgccctc aggcgctctc catcctacaa ttaaacgata tcaaaagccc
     3421 ttcacttttg tcaacctccc cctccccgaa gcgcgggccg aacacccgcc acgctctctc
     3481 ttgggtttcc catgttctga gacaagcgat gaccttttca gacaatcctg aggaccttgg
     3541 cgccccgtat ctttctttcc ttgagttcaa gaagcgccct gttcgcctca tcgagagaga
     3601 agatctccac ctcgggccgg atgggtatgg atgccgccag ttcaaggaat tcccggacgt
     3661 cgcgggaagc cacgttggcg acgctcttta tctctttttc gagccagagg tgctccggat
     3721 aggagagcgc gaggagctct tccttgtcga tctcctcctt tcgtatagcg ttgatgacga
     3781 gacgccctcc ccgttccagg tttccgagcg cgtgcaccac ggtaccccag acaggggtgg
     3841 tgt
//
LOCUS       DAOQTR010000170         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_26984_length_3824_cov_4.468559, whole genome shotgun sequence.
ACCESSION   DAOQTR010000170 DAOQTR010000000
VERSION     DAOQTR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26984_length_3824_cov_4.468559"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PKN85_05200"
     CDS             complement(<1..1122)
                     /locus_tag="PKN85_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNT43835.1"
                     /translation="MTSSLEHHDVDTDRPDLFTIEVNEEEGSVFVTIHQTSGCPSPDE
                     VVRGLEERNLPYFIDRATIAKELAHKATDQPFRAAFAKDEEFEIKVDATQRVATLSLK
                     QGYGGRGVTMVDVQLRLNEMGIVSGVDLDRAALALSEKTYDKPIVIARATEPVPGTDA
                     AIDCLFPLQFSMQPKELENDKVDFRELKLIYAVAAGDILARKIPATRGEPGMTVVGTA
                     IPAKPGKDIVFTAGRNTIISPDGMEVTSAIAGQPFLKGRSVFVEPVYSIKGDINYSVG
                     NIDFKGSVKIEGNVVSGFSIKATESIEVNGVVEDCSLEAGMDISVKGGVLGADRGIMK
                     AGRDLSALFVENCHVEAGRNIVVGDTLNSELFAGDSIDVA"
     gene            1234..2262
                     /locus_tag="PKN85_05205"
     CDS             1234..2262
                     /locus_tag="PKN85_05205"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT43836.1"
                     /translation="MTNQGRQRDAIGKGKVWAGALTYLAVILMTAAGYGQPALKKITF
                     IPQWSPQAQFAGYYAAYEKGIYARHGLEVKILRGGAHRPSSELLEKGEADMASIWLST
                     ALQKRSSGVRLVNVAQVVQRSSLMLIAKKSRGIRRPEDLEGKKVGLWGDDFRIQPTAF
                     FKKYKVTVKVVPQSYSVNLFLRDGVDAASAMWYNEYHTVLNAGINADELTSFFYSDYG
                     LNFPEDGIYIMEETLKKDPAAVCAFVSASLEGWRWAFDHPEEAIDIILKYMVEANVPA
                     NKVQQRWMLERMRDVITPSGSDRAAMGVLKKEDYERVGKELKDGGLIGDVPGMDAFAF
                     TCTGYVKK"
     gene            2249..>3824
                     /locus_tag="PKN85_05210"
     CDS             2249..>3824
                     /locus_tag="PKN85_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNT43837.1"
                     /translation="MLKNKGIAFKLVLFFTLSSAAIFAAIFSYNYLVSRRMIQKGIEE
                     NSANLITTTTSRIEVLLTSTQKVPQNTAYLLENTNYDEAELFKVIYAIVEKNPEIYGA
                     AIAFEPYAFDRTKKHFAPYFYRNGEGISFKYLDRFYDYFHWDWYQIPRELEEPQWTEP
                     YFGEGGGILMSSYAVPFYRVVDGTRRLTGVVVVDISLEELRKIVSSIRILKSGYGFLI
                     SKNGTFVTHPIKEHIMNETIFSVAEERKEPTLRETGRRMIRGERGYSATPVKSAVTGK
                     SCIMTYVPIKSNGWSLGVLFPYDELMADITNLNHVVLALGITGLLLLALAVVFIARSI
                     TKPLTEMADIAGRIGTGDLEAQVPMVRTKDEVGKLASALDCMKTSLKDYIREITDTTA
                     ARQRMESELKIARNIQMGILPKVFPPFPDRKDFDIHALTIPAREVGGDFYDFFLIDRD
                     HLCIVIGDASGKGIPAAIFMAFTKTLIKAKASVSVQTGTLMEEVNDELSRGNPADMFI
                     TVFCAILNTATGELRYT"
BASE COUNT          824 a         1086 c         1115 g          799 t
ORIGIN      
        1 ggcgacatcg atggaatcgc ccgcgaacaa ttcggaattg agggtatcgc cgacgacaat
       61 gttgcgtccc gcctccacgt ggcagttctc gacaaagagc gcggagagat cccttcctgc
      121 cttcatgatg cccctgtccg cgccgagcac gccgcccttg acagagatgt ccatcccggc
      181 ttcaagcgag cagtcttcga cgacgccgtt cacctcgatg ctttccgtcg ccttgatgga
      241 gaacccggac acgacattgc cctcgatctt cacgctgccc ttgaaatcga tgttgccgac
      301 ggagtagttg atatccccct tgatgctgta gacgggttcg acgaacacgg accttccctt
      361 caggaacggc tgcccggcta tggcagaggt cacctccatg ccatcggggg agatgatcgt
      421 gttcctcccg gcggtgaaaa cgatatcctt gccgggtttt gccggtatcg ccgtgcccac
      481 gacggtcatg cccggctcac cccgggtcgc cggtatcttc ctcgccagga tgtcccctgc
      541 ggccactgca tagatgagct tgagctccct gaagtcgacc ttgtcgttct ccaattcctt
      601 cggctgcatg gagaattgaa gagggaacag gcaatcgatg gcggcgtccg tcccggggac
      661 gggctccgtc gccctggcga tcacgatggg cttgtcgtag gtcttctcag acagggcgag
      721 ggccgcccgg tcgagatcta ctcccgagac gatccccatc tcgttgagcc tgagttggac
      781 gtccaccatg gtgacccccc tgccgccgta cccttgtttc aggctcaatg tggcgacgcg
      841 ttgggtggca tcgaccttga tctcgaactc ctcatctttt gcgaaagctg cacgaaaggg
      901 ctggtccgtg gctttgtgcg ccagttcctt tgcgattgtc gctctgtcga tgaagtaggg
      961 cagattccgc tcctcaaggc cccgtacgac ctcgtccggt gaaggacatc ccgatgtctg
     1021 gtgaatggtg acgaagacgc tgccttcttc ctcgttcact tcgatcgtga ataggtccgg
     1081 cctgtcggtg tcaacgtcgt ggtgttcgag cgatgatgtc atgacagtta ctccccctgt
     1141 atgattaaat atcggaagaa agaagacact cttaaggata gcggattttt gtgttgactc
     1201 gcggcccgct atcaatcatc ataataactg atcatgacga accagggcag acagcgcgac
     1261 gcgatcggga aggggaaggt gtgggccgga gcgctgacat atcttgctgt tatcctcatg
     1321 acggcggccg gatatggcca gcctgccttg aaaaagatca ccttcatccc ccagtggagt
     1381 ccgcaggccc agtttgccgg ttattatgcc gcctacgaga agggcattta cgccaggcac
     1441 ggcctcgaag tcaagatcct ccggggaggt gcccacaggc cgtcatcgga actcctcgag
     1501 aagggtgagg ccgacatggc cagtatctgg ctttccacgg ccctccagaa gcgttcgagc
     1561 ggtgtgaggc tcgtcaacgt cgcccaggtg gtgcagcggt catcgcttat gctcatagcg
     1621 aagaagtcgc ggggcatcag aaggccggag gaccttgagg ggaagaaggt ggggttgtgg
     1681 ggtgacgact tccgcataca gccgacggca ttcttcaaga agtacaaggt gacggtcaag
     1741 gtcgttcccc agtcgtattc cgtgaatctc tttcttcggg atggcgtcga cgccgcctcg
     1801 gccatgtggt acaacgagta tcacacggtt ctcaatgccg ggatcaacgc cgacgagctg
     1861 acatccttct tttacagcga ttacggcctg aactttccag aggacgggat ctacatcatg
     1921 gaggagacgc tgaagaagga ccctgcggcg gtatgcgcct ttgtgagcgc gtctctcgag
     1981 gggtggcgct gggccttcga ccacccggag gaggccatcg atatcatcct caagtacatg
     2041 gtcgaggcga acgtgccggc caacaaggtg cagcagcgat ggatgctcga aaggatgagg
     2101 gacgtgatca ctccctccgg cagtgacagg gcggcgatgg gtgtcctcaa gaaggaagat
     2161 tacgaaaggg tggggaaaga gctgaaggat ggcggtctta tcggggacgt gcccggcatg
     2221 gacgcctttg cttttacgtg tacgggatat gttaaaaaat aaaggcatcg ccttcaaact
     2281 ggtccttttt ttcaccttaa gcagcgccgc catctttgcc gccatattca gctacaatta
     2341 cctcgtctcc cgcaggatga tccagaaggg catcgaagag aactcggcga accttatcac
     2401 caccacgacg agcaggatcg aggttctcct gacatccaca cagaaggtgc cccagaacac
     2461 ggcatatctt ctcgagaaca cgaattacga tgaggccgag cttttcaagg tcatctatgc
     2521 catcgtggag aagaacccgg agatatacgg cgcggcgata gccttcgaac cctacgcctt
     2581 cgacaggaca aagaagcact tcgctcccta tttctacagg aacggcgagg ggataagctt
     2641 caaatatctc gacaggttct atgactattt tcactgggac tggtatcaga tcccccggga
     2701 gctcgaagag ccgcaatgga cggaacccta tttcggcgag ggcggcggca tcctcatgtc
     2761 atcctatgcc gtgcctttct acagggttgt cgacggaacg cggaggctga ccggtgtggt
     2821 cgtcgtggac atctccctcg aggaactcag aaagatcgtc tcctcgataa ggatcctgaa
     2881 gtccgggtat gggtttctca tatcgaagaa cggcacattt gtcacccatc ccatcaaaga
     2941 gcacatcatg aacgagacga tcttctccgt cgcggaagag aggaaggaac ccacgctgcg
     3001 cgaaacggga cggaggatga tacgggggga gaggggttat tccgcgaccc ctgtgaagag
     3061 cgcggtgacg gggaagtcat gcatcatgac ctatgtcccg atcaagtcga acgggtggtc
     3121 cctgggcgtt ctctttccct acgacgagct tatggcggac atcaccaatc tcaatcatgt
     3181 ggtcctcgcg ctgggtatta ccggcctttt gcttctcgcc ctcgcggtcg tattcatcgc
     3241 ccgctccata acgaagccgc tgacggagat ggctgacata gcggggagga taggcacggg
     3301 cgacctcgag gcccaggtac ccatggtgcg gacgaaggac gaggtgggaa agctcgcatc
     3361 ggccctcgat tgcatgaaga cgtcgctcaa ggactacatc cgggagatca ccgatacgac
     3421 ggccgcgcgc cagcgcatgg agagcgagct caagatagcc aggaacatcc agatgggcat
     3481 ccttcccaag gtgttccctc cctttcctga caggaaggat ttcgatatcc atgcccttac
     3541 cataccggcc cgcgaggtgg gcggggactt ctacgatttc ttcctcatag acagggacca
     3601 cctctgcatc gtgataggcg acgcctccgg caagggaata ccggcagcca tcttcatggc
     3661 gttcacgaaa acccttatca aggcgaaggc ctcggtcagc gtccagacag gtacactcat
     3721 ggaggaggtc aatgacgaac tgagcagagg gaaccccgcg gacatgttca tcaccgtctt
     3781 ctgcgccatc ctgaacacgg ccacgggcga acttcggtat acga
//
LOCUS       DAOQTR010000171         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27039_length_3818_cov_3.664895, whole genome shotgun sequence.
ACCESSION   DAOQTR010000171 DAOQTR010000000
VERSION     DAOQTR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27039_length_3818_cov_3.664895"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1453)
                     /locus_tag="PKN85_05215"
     CDS             complement(74..1453)
                     /locus_tag="PKN85_05215"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT43838.1"
                     /translation="MFSGTTASQKILIIDDDRSVRLVLATLLRKNGFVPLEASGGQEG
                     ISSLRSEHPRCVLLDLKMPGMDGIETLHALKKIDQFVPVIIITGYADIPTAVQTIKLG
                     AYDFLTKPPQVDKLILTIKRAIEAYSLQEALNLLDDTMLGSLESLFGRSDAMKSVIHH
                     IRQVSRTDFSVIIQGDTGTGKSVVAQTIHDLSKRARNPFQSVDVGVIPENLIESELFG
                     HEKGAFTGADRKKTGFFEIAHKGTIFIDELENTPPLLQNKLLRAVEEKRIYPIGSTKP
                     VDIDVRIIAASNADIKDAVREKRFREDLFFRLSEYMITMPRLRDRPSDIPFLAMKLMT
                     AASNELEKQMRELTPEAAELLMTYPWPGNIRELKNVIRRAVLTCRDGVIRPEDIEFIV
                     GETVSRQDNGTFLMPLKQVALQATREAETEVIKRALTITGGNKSRAARLLEIDYKTLL
                     TKVKDYRIS"
     gene            complement(1548..2216)
                     /locus_tag="PKN85_05220"
     CDS             complement(1548..2216)
                     /locus_tag="PKN85_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43839.1"
                     /translation="MSLNELAELLLAALYEEMQNLKHTNYFLSVDDITASLGVEDRER
                     VVEACHLLEEKSYVLLTYDHLTSLSAFITPVGENFVREGGETGIITEYQRYRAAAGAG
                     APNPDDPGAGFQPPLASSFQPPPSSVPSPPTPAPSEQSAAPLSGEVASHIIASMELII
                     RNDPSLSESAKNDLVIDLRTLELQMSRGSIGRSVVDAVTAELRGVPALIPLIDFLLTM
                     KGVG"
     gene            complement(2364..>3818)
                     /locus_tag="PKN85_05225"
     CDS             complement(2364..>3818)
                     /locus_tag="PKN85_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43840.1"
                     /translation="YLPMQTIEAHGVSLERSLADMRVVPGPSSAGEALEGKALAALAF
                     FDIRWVYQLRFLRVLWNNNAESWDSVRNAMTENEGGFAWLAQDSLRVSNYDKARSFLT
                     LALERKPQDYKARCVAGLLNMELGDAPAAIADFRKALESAGTNVHKIYASLILARLYK
                     LSGDTVRFRHMLGEIGRLTGGCVEAAYEEILFKLEEKDEKGAMKKLVKLVRDDPGFFV
                     TVLIDPELAPYRNTIYAHLTELMAEAKNDAISHLEDARKELNRCRPLLPLSALKTIEP
                     ALQEIEMSIHSESYLGCLDVPRRCGSIRVMCKNAIEEQSRTITGTMKDLRARLNNADA
                     FLKTYRYSCLSEKHRGRLVYLRRSMERIGDVRYFESSVEFESCHQMCREISDELTSIE
                     KRLEFLDLSANLIQMCLKFLKYSSILFSIVFFFGIFIFPLLSEEVNTVLSRMDISVFS
                     SVWSLQKAFLIFGGITSLVAAFLMTIRQVLTGDK"
BASE COUNT          793 a         1034 c         1144 g          847 t
ORIGIN      
        1 aggcccgttc atgaaaaaga aatcaattga cattcaacac agttgctgga acctttgaac
       61 tcttgaaccg tttctaggat attctgtagt ccttcacctt cgtgaggagg gtcttgtagt
      121 caatctcgag gagccttgcg gcacgggatt tgttgccgcc ggtgatcgtg agcgcccgct
      181 tgatcacctc agtttccgcc tcgcgcgtgg cctggagggc gacctgcttc agaggcatga
      241 ggaaagtgcc attgtcctgg cgggataccg tctctccgac tatgaactcg atgtcctcgg
      301 gcctgataac accgtcacga caggtgagca ccgccctgcg gatgacgttc ttcaactccc
      361 ggatattgcc gggccatgga taggtcatga ggagctccgc ggcttcgggt gtcagttccc
      421 gcatctgctt ctcgagttcg ttggaggctg cggtcatgag cttcatggcg aggaaaggta
      481 tgtcggaggg ccggtcacgg aggcgcggca tggtgatcat gtactcgctt agccggaaga
      541 acaggtcctc gcggaatctc ttctccctga ccgcatcctt gatgtccgca ttggatgccg
      601 ctatgatccg gacatcgata tcaacgggct tcgtgctgcc gatcgggtag atcctcttct
      661 cctcgacggc cctgagaagc ttgttctgga ggaggggagg tgtgttctcc agttcgtcga
      721 taaagatggt tcccttgtgg gctatctcaa agaaacccgt ctttttcctg tccgctcccg
      781 taaatgctcc cttctcatgg ccgaagagct cgctttctat gagattctcc ggtatcactc
      841 cgacatcgac ggactggaag gggttcctgg ctctcttgct cagatcgtgt atggtctggg
      901 ccaccacgga ttttcccgtc cctgtgtctc cctggatgat aacggagaaa tccgtcctgg
      961 acacctggcg tatatggtgg atgacgctct tcatggcatc gcttctgccg aaaagggact
     1021 ccagggaacc cagcatcgtg tcatcgagca gattgagagc ctcctggagc gaataggctt
     1081 cgatggcgcg cttgatcgtc aggatcaatt tatcgacctg gggaggcttg gtgagaaaat
     1141 cgtaggcgcc aagtttgatc gtttgcacgg ccgttgggat gtcagcgtag ccggttatga
     1201 tgatcaccgg aacgaactgg tctatcttct tgagagcgtg aagtgtttct ataccgtcca
     1261 tacccggcat cttgaggtcg agaaggacgc accgggggtg ttcgctcctc agggaagaga
     1321 tcccctcctg accgccggag gcctcgagag gcacgaaacc gttcttcctg agcagggtgg
     1381 ccagaaccag ccgcaccgat cggtcgtcat cgattatgag tatcttctga gatgctgtcg
     1441 tcccggaaaa cataattttt tcaatgatga tacagcacaa gacaaaggga tgtcaatcag
     1501 gaatgtccga caggtccgcc ccgtctgccg cttaatgggg gggtgggtca accaactccc
     1561 ttcattgtca gcaggaagtc gataaggggg atgagcgcgg gcacgcctct gagttccgcc
     1621 gtgacggcat ccaccacgga cctcccgatg gagcctctcg acatctgaag ctccagcgtc
     1681 ctcagatcta tgacaaggtc atttttcgcg ctttccgaca gcgacggatc gttgcggatg
     1741 atgagttcca tactggcgat gatatgactg gcgacctcgc cggagagcgg cgcggcgctc
     1801 tgctcagagg gggcaggtgt cggcggggag ggcacggatg agggaggggg ctgaaaagac
     1861 gacgcgagag gcggttgaaa cccggcaccc gggtcgtcgg gatttggcgc gccggcacca
     1921 gcggccgctc tgtatcgctg gtattcggtg atgatccccg tttctcctcc ctcgcgcaca
     1981 aagttttctc cgacgggggt gatgaaggcg ctcagagatg tcaggtggtc gtaggtcagg
     2041 agaacgtagc tcttctcctc gagcagatgg cacgcctcga caacgcgctc gcggtcctcg
     2101 acacccaggc tggccgttat gtcgtcgaca gaaaggaagt aattcgtgtg cttcaggttc
     2161 tgcatttcct cgtacaatgc ggcaaggagc agttcggcaa gttcgttcag actcaagtca
     2221 tccatcgggc agtagtatag caagtgttgc aggggggagt aaagggcaat gtgcacaaga
     2281 aaatgttccg agtcgcgggt tccgagtcgc gggctcaaga agaaacgtca tccggggcac
     2341 aggatgttga agtgtatgga aggttatttg tcgccggtaa ggacctgcct gatggtcatg
     2401 aggaaggccg cgacgaggct cgtgatgccg ccgaagatga ggaatgcctt ctggagcgac
     2461 cagacgctgg aaaagaccga gatatccatc cgtgagagca cggtgttgac ctcttcggaa
     2521 aggaggggaa atatgaatat cccgaaaaag aagacgatgg agaagagaat ggaggaatac
     2581 ttgaggaact tgaggcacat ttggatgaga ttggcggaaa ggtccaggaa ctccagcctt
     2641 ttctcgatgg aggtgagctc gtccgatatc tcccggcaca tctggtggca actctcgaac
     2701 tccaccgaag actcgaaata acgcacgtcg cctatacgtt ccatggaccg cctcaaatag
     2761 accaggcgtc cccggtgctt ctccgacagg caggagtacc gataggtctt caggaaggcg
     2821 tcggcattgt taagccgagc acgcaggtcc ttcatcgtcc cggttatcgt ccgggactgc
     2881 tcttcgatgg cgttcttgca catcactctg atcgaaccgc accggcgggg cacgtcgaga
     2941 caacccaggt aactttccga gtgtatcgac atctcgatct cctgcagggc gggctcgatg
     3001 gtcttcaatg ccgacagagg caggaggggg cggcagcggt tgagctcctt cctggcgtcc
     3061 tcaaggtggg agatggcgtc gtttttcgct tctgccatga gttctgtcag gtgcgcgtag
     3121 atggtgttcc tgtaaggcgc gagctcggga tcgataagga cggtcacgaa gaagccgggg
     3181 tcgtccctga cgagcttcac gagtttcttc atggcccctt tttcatcctt ctcctcaagc
     3241 ttgaaaagga tctcttcgta ggcggcttcg acgcagccgc cggtaaggcg gcctatctcg
     3301 ccgagcatgt gcctgaacct cacggtgtcc cctgacagct tgtacagcct cgctagaatg
     3361 agggaagcgt agatcttatg gacgttcgtc cccgccgact ccaacgcctt cctgaagtcc
     3421 gcgatcgccg ccggagcgtc tccaagctcc atattgagaa gccccgctac acagcgggcc
     3481 ttgtaatcct gaggtttcct ctcgagggcg agtgtgagaa aggacctggc tttatcgtag
     3541 ttggaaacgc ggagcgagtc ctgcgccagc caggcaaaac caccctcgtt ctccgtcatg
     3601 gcattccgga ccgaatccca ggattccgcg ttattgttcc agaggacgcg caggaaacgg
     3661 agctgataga cccaccgaat gtcaaagaat gccagcgccg ccaatgcctt tccctcgaga
     3721 gcttcgcccg ccgatgaagg gccggggacc acccgcatat ccgccagcga tctttcgagg
     3781 gagaccccat gcgcctcgat cgtctgcatg ggaaggta
//
LOCUS       DAOQTR010000172         3815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27072_length_3815_cov_3.505319, whole genome shotgun sequence.
ACCESSION   DAOQTR010000172 DAOQTR010000000
VERSION     DAOQTR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27072_length_3815_cov_3.505319"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1930)
                     /gene="asnB"
                     /locus_tag="PKN85_05230"
     CDS             complement(35..1930)
                     /gene="asnB"
                     /locus_tag="PKN85_05230"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNT43841.1"
                     /translation="MCGITGVLNLNGGRPPEPSRLVRANDALSHRGPDDSGTFTDGQV
                     GLAMRRLSIIDVARGHQPLSNENGAISIVFNGEIYNHLELREELVRRGHVFRTRSDTE
                     TIVHAYEQWGMEGCLSRLRGMFAFAIWDSSRRALFLCRDRMGIKPLYYAEEGGRLYFS
                     SEIRGILLQSGIPCRVDMAAVDAFLTVGFVPNPRTICKGIRKLPPAHFLTVRNGTVTM
                     RKYWELAYSEGNGSTVRELTEEFQDLLAECIGMHLMSEAPLGALLSGGIDSATIAAFA
                     QRSLSTRLKTISISFDSCRLNEADAARETAGALGTDHVPVAFGKDSMDDYPEALYYLE
                     EPLAEPVFVTEYNMHRAVRQKGLKVVLTGEGSDELLAGYHWHRGEVWSRPLLGLPFFL
                     RAFVAEGLLLRSRGAGAMGMYRVLREGRSEAQDQYRAWLTIGDPKIKERLLAREVSAA
                     LKGNGGSLLDSWKDHLPAVAGRPLSDQMLWLQSRTRMVDDINHSLDRMSMAHSVEARV
                     PFLDHKLWEFCASVPFRLKIDGTYFDLTEKFLLRNAVKGIVPEGARLRRKKGLAAPYE
                     TWLMGSRLPDWAETALSEAEIKKVGIFEAKEVARLRREHQAGLPDRATLLMGVLALQT
                     WSRLFTG"
     gene            complement(1968..3191)
                     /locus_tag="PKN85_05235"
     CDS             complement(1968..3191)
                     /locus_tag="PKN85_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43842.1"
                     /translation="MEKVIVLTARELGYQVVKALAAEGIGSIVIYGHEPDEIAQFSKH
                     VIETHEIPGYFDRQGVLTDFLLERKDEWKGTLIIPTDDYGVKFLAANRDRLSAHYAIP
                     APDLDVVNTIVDKKSLYAVARRIGVPVPAIYSPRTMDELRSLEGVLRFPCLVKPGLAH
                     LFIREFGLKMFEVHSFVDLVRHYRELTDDFTSDRFELMICDIIPGPDSRQMVQYVSYM
                     DRSGEVLASMTSRKLRQDPPRYGQGRVAISERVDGVDEISRRLLAELGYHGFSEIEWK
                     YDPRDGTYKLIEINPRFIFYTGLCVACGINFPYIQYADLVLGRKIRIGSFKENVYWIH
                     EYADALHTVLNHRLEDLSLRDYVRPYLGKKCFAIFDPRDCRPFFEQWKQHAGNVIRRW
                     RNARTSPFRINGVSP"
     gene            complement(3179..>3815)
                     /locus_tag="PKN85_05240"
     CDS             complement(3179..>3815)
                     /locus_tag="PKN85_05240"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNT43843.1"
                     /translation="VGSLKKDCDFLIMFLHWGDDHNIFINADQQEAGRRLIDQGADLV
                     IGHHPHVTQGSEVYKGKHIFYSLGNFIFTPREEYESLPYSIRYEDCRENVLFQRPECK
                     IGMYVRVVFDRTGYEVAGVCPVYREETLPAVLPASLASFFDGMLEQMNSQVAGSAYEL
                     NEAGRRMILTRYTLPLILTHPLYWPVFFRKLNVGKAVRFVRQGRVGKLWKK"
BASE COUNT          725 a         1195 c         1112 g          783 t
ORIGIN      
        1 acatcgacgg gacatgtttc gcggtacgca acggtcaccc cgtgaagaga cgggaccagg
       61 tctggagcgc caggacaccc atgagcaggg tggcccggtc cggcaggccg gcctggtgtt
      121 ctcttctcag tcttgcaacc tcttttgctt cgaagattcc aaccttcttt atctcggctt
      181 ccgaaagcgc cgtctccgcc cagtccggca gacggcttcc catgagccat gtttcgtagg
      241 gcgccgccag gcctttcttc ctgcggagcc tcgccccctc gggtacgatg cccttcaccg
      301 cgttcctgag caaaaacttc tctgtgagat cgaaataggt cccgtcgatc ttcaaacgga
      361 aggggaccga ggcgcagaac tcccacagtt tgtgatcgag aaagggtacg cgcgcctcaa
      421 cggaatgggc catgctcatc cggtccagcg agtggttgat gtcgtcgacc atgcgcgtcc
      481 gcgactgcag ccagagcatc tggtcgctca agggccgccc cgccacggcc ggaaggtggt
      541 ccttccagga atcgagcagc gatcctccat tgccttttaa cgccgcggag acctcgcggg
      601 caagcagacg ctccttgatc ttcgggtcgc cgatggtgag ccacgcgcgg tactggtcct
      661 gcgcctcact gcgcccctcc cgcaggaccc tgtacatccc catggcgccg gcaccgcggg
      721 aacgcaggag aagaccctcg gccacgaagg cgcgcaggaa gaagggcagt cccagaagag
      781 gtcttgacca gacctcgccc cggtgccagt ggtaaccggc cagcaactcg tcggacccct
      841 cgccggtgag cacgaccttg aggccctttt gccgcaccgc cctgtgcatg ttgtattcgg
      901 tgacgaacac cggttccgcc aggggttcct ccagatagta gagggcctcc gggtaatcgt
      961 ccatgctgtc cttgccgaaa gcgacgggaa cgtggtccgt tccgagagca ccggccgttt
     1021 cccgggcggc atcggcctcg ttgagacggc agctgtcgaa gcttatggat atggtcttca
     1081 agcgtgtcga aagagacctc tgcgcgaagg cggctatcgt cgccgaatcg atcccgcccg
     1141 acaggagcgc cccgagaggc gcctcgctca taaggtgcat cccgatgcat tccgccagaa
     1201 ggtcctggaa ctcttcggtc agctcccgaa cggtgctgcc gttgccctcg ctgtaggcga
     1261 gctcccagta tttcctcatc gtgactgtcc cgttcctcac ggtaaggaag tgggcggggg
     1321 gaagcttcct tattcccttg cagatggtgc gcgggttcgg cacgaacccg acggtgagaa
     1381 aggcatcgac cgcggccatg tccacgcggc agggtatccc cgattgcagc aggataccgc
     1441 ggatctcgct ggaaaagtag agcctgcccc cctcttccgc gtagtacagg ggcttgatcc
     1501 ccatgcggtc cctgcacagg aaaagggcgc gcctggagct gtcccagatc gcgaacgcga
     1561 acatcccccg caatctcgac aggcagcctt ccatgcccca ctgttcgtag gcatggacga
     1621 tcgtctccgt gtccgacctg gtcctgaaaa cgtgccctcg ccttacgagc tcttctctca
     1681 actccagatg gttgtatatc tcgccattga agacgatgga tatggctcca ttctcattgg
     1741 acaacggttg gtggcccctc gcgacgtcta tgatgctcag gcgacgcatc gcaagcccca
     1801 cctgcccgtc ggtgaaggtc cccgaatcat cgggccccct gtgcgagagc gcgtcgttgg
     1861 cccttacgag ccgggacggc tccggcggcc tcccgccgtt aaggttcaga acaccggtaa
     1921 tgccacacat actgcctcct tccaggcgat gacaccgtga acgtttatca tggtgaaaca
     1981 ccgtttattc tgaacgggga ggtcctcgcg ttcctccacc tcctgatcac attgccagcg
     2041 tgttgtttcc actgttcgaa gaagggcctg cagtccctcg gatcgaatat ggcgaaacac
     2101 ttcttcccca ggtagggcct cacgtagtcc ctgagcgaaa ggtcttccaa gcgatggttc
     2161 agcacggtat ggagtgcgtc cgcgtattcg tggatccaat agacgttctc tttgaaggag
     2221 ccgatcctga tcttcctgcc gagaacaagg tccgcgtact ggatgtacgg aaagttgata
     2281 ccgcaggcga cgcagagtcc cgtgtagaat atgaaccggg gattgatctc gatgagcttg
     2341 tacgtcccgt cgcgggggtc gtacttccac tctatctcgg agaacccgtg atagccgagc
     2401 tccgcaagga gcctgcggct gatctcatcg acgccgtcaa ccctctcgct tatcgccacc
     2461 ctcccctgcc cgtaccgcgg cgggtcctgc ctgagcttcc tcgacgtcat ggaggcaagc
     2521 acctctcccg acctgtccat gtaagaaacg tactggacca tctgcctgct gtccgggccg
     2581 ggaatgatat cgcatatcat cagttcgaac ctgtcggacg tgaagtcgtc ggtgagttcc
     2641 ctgtagtgcc tcaccagatc gacgaaactg tggacctcga acattttgag gccgaattcc
     2701 cgtatgaaga gatgcgcaag acccggtttt accaggcagg ggaacctgag aaccccttcc
     2761 agggaccgga gctcgtccat cgttcggggg gagtaaatgg cgggcaccgg gacaccgatg
     2821 cgccgggcga cggcatagag ggacttcttg tccacaatgg tgtttacgac atcgagatcg
     2881 ggcgcgggga tagcgtagtg agcggacagc ctgtccctgt tggccgcgag gaatttgacc
     2941 ccatagtcgt cggtggggat tatgagcgtt cccttccatt catccttcct ctcgaggagg
     3001 aagtcggtca ggacgccctg cctgtcgaag taaccgggta tctcgtgggt ctctatgacg
     3061 tgcttcgaga actgggcgat ctcgtcgggc tcgtgcccgt agatcacgat gctgccgata
     3121 ccctccgcgg cgagggcctt tacgacctgg tacccaagtt cccgcgctgt cagaacgatc
     3181 actttttcca tagctttccc accctccctt gccttacgaa gcgcacggcc ttccccacat
     3241 tgagctttct gaagaaaacg ggccagtaaa ggggatgcgt caggataagg ggcagggtgt
     3301 accgggtgag gatcatcctc ctgcccgcct cgttgagctc atacgccgat cccgccacct
     3361 ggctgttcat ctgttccagc ataccgtcaa agaaggatgc gagagaggca ggcagcacag
     3421 ccggaagtgt ctcctcccgg tacacgggac acaccccggc gacctcataa cccgtcctgt
     3481 cgaagaccac cctcacgtac atgccgatct tgcactcggg acgctggaag aggacattct
     3541 ccctgcagtc ctcgtagcgt atgctgtagg gcaggctctc gtactcctcc cggggcgtga
     3601 agatgaagtt tcccagggag tagaatatgt gcttcccctt gtagacctcg ctgccctgcg
     3661 tcacgtgggg gtggtggccg atgacgaggt cggcgccctg gtcgatgagc cgtctgcccg
     3721 cctcctgctg gtcggcgttg atgaagatgt tgtggtcgtc gccccagtgg aggaacatga
     3781 tgagaaaatc gcagtccttc ttcagggaac ccaca
//
LOCUS       DAOQTR010000173         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27347_length_3788_cov_3.859630, whole genome shotgun sequence.
ACCESSION   DAOQTR010000173 DAOQTR010000000
VERSION     DAOQTR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27347_length_3788_cov_3.859630"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKN85_05245"
     CDS             <1..367
                     /locus_tag="PKN85_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43844.1"
                     /translation="QVTKLSITGDGKAGVESEIEGGRRTSEVSLPALFTCQKGLNEPR
                     VPLITGVMKAMKTQIDILDPASIEAPFGPIDRESSKITVLSYEAPAGRPSVRIMEGAS
                     PEEKAQNLVRALKDEAKVL"
     gene            383..1363
                     /locus_tag="PKN85_05250"
     CDS             383..1363
                     /locus_tag="PKN85_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNT43845.1"
                     /translation="MTRDVFVVVECKDSKPRKASFELLSIGREMASRFSSTLFAVVLG
                     SGLADAADAVARFAGKTILVDDAALAEYGWDTYGSVLETILKKYEPMVVLGTSSVTGK
                     DLFPRMAARLSAAMVSDAVGVDMAGEGIRIKKPLYGGKVISWLAPKADSTVIVTFRPN
                     SFAVADAGDAGEIIEEAAAVAGNSAMKTMSSERKTSGKVDLQEADFIICGGRGMKAAE
                     NFAMLDEIADLMGGRTGASRTVIDSKWRDYDDQIGKSGKTVSPKLYIGCGLSGALHHI
                     MGMDTSKVIVAINKDPNAPIFQYADYGIVDDVFAILPALKDELKKTREET"
     gene            1369..2658
                     /locus_tag="PKN85_05255"
     CDS             1369..2658
                     /locus_tag="PKN85_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43846.1"
                     /translation="MDKIDVVIVGAGLAGLSCAWVLSGHGLQVIVVERGDFAGSKNVT
                     GGRLYLKPVSGMASEMLDGAPFERKVVRERWSLLGSTNSVSIDHTSDRFRDEDHSYTV
                     LRARLDRWLSERLMARGVFVVPKYRVDDLLWENGAVAGIRAGAEEIPAHVVVAADGVL
                     SFMGRKAGLMPPLSPKTCAVGMKEVLELSEEKINDRFNVGDGEGVAHLFLGDVTKGVF
                     GGGFLYTNKDTVSLGVVAGIGAMMGRIPAMDASTLLDMFKERYEMRRLLEGAKIVEYS
                     AHVIPEAGYDGIGKLCGNGILLAGDAAGLALNMGVTVRGMEFAIASGMAAAEAIVEAK
                     ASGDLSEKGLSRYGERLKETFVLKDLEASRNMPGYIDNGSFFSYYPETFPGLLEKIMW
                     FDESPKGSLGKTLWKELRSSGMLSVKRLIELYRIRNI"
     gene            2670..2948
                     /locus_tag="PKN85_05260"
     CDS             2670..2948
                     /locus_tag="PKN85_05260"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT43847.1"
                     /translation="MKIDEKLALDAFKTDRESHILIDHDICRSRCTLRHCLNICPGHL
                     YSFNEEGNEIVVEYAGCLECGTCMVACTEGAIQWSYPRGDFGVQYRYG"
     gene            3198..>3788
                     /locus_tag="PKN85_05265"
     CDS             3198..>3788
                     /locus_tag="PKN85_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43848.1"
                     /translation="MSESNSRKWIDSKELLTRSAISRATLNNYIKLGVLPRPVVKSPA
                     DSQKGTKKIGYFPDTVLDTISEIKKLKEKGKPMEAIAREMRTSSPESGYEGPERRGSV
                     SQDAGERVPVESVAVPGGGLRLTLDEVTTPAYLLNYQFEIEWINEAAEREIFGKPIRS
                     IRDVEFRNVFRLFLGWEFNHFVKNWEEIFAYHMAFYK"
BASE COUNT          828 a         1013 c         1203 g          744 t
ORIGIN      
        1 ccaggtaacg aaactcagta tcaccggtga cggcaaagcc ggggtcgaaa gcgagataga
       61 ggggggcaga aggacgtcgg aggtctcgct gccggccctt ttcacctgtc agaaaggcct
      121 caacgagccg agggtgcccc tcattacagg cgtgatgaag gccatgaaga cacagataga
      181 tatcctcgat cccgcgtcca tcgaggcgcc cttcggtccc atagacaggg agtcgtcgaa
      241 gatcacggtg ctgtcctacg aggcaccggc cggccggcct tcggtccgca tcatggaggg
      301 agcgtcaccg gaagagaagg cgcagaacct cgtcagggcg ctcaaggatg aagcgaaggt
      361 cctgtagaga ggaggattgc acatgaccag ggacgtattt gttgttgtgg aatgcaaaga
      421 ctcgaagccg agaaaggcat cgttcgaact gctgtccatc ggcagggaga tggcctcgcg
      481 gttctcgtcc acgctgttcg cggtggtgtt ggggtccggc cttgcggacg ccgccgatgc
      541 cgtcgcccgg tttgcgggta agacaattct tgtcgacgat gctgcgctcg cggagtatgg
      601 gtgggatacc tatggatcgg tcctcgagac tatcctgaag aagtacgagc ccatggtcgt
      661 tctgggaacg tcgtcggtga cgggcaagga cctctttccc aggatggcag cgcgactttc
      721 cgccgcgatg gtgtccgacg cggtcggtgt cgacatggcg ggggaaggga taaggataaa
      781 gaaacccctg tacggcggca aggtgatctc ctggctcgcg ccaaaggcgg actcgacggt
      841 catcgtcacc ttcagaccca attcctttgc cgtggccgac gcgggcgacg cgggcgagat
      901 catcgaggaa gcggccgcgg tggccgggaa ttcggcgatg aagacgatgt cgtccgaaag
      961 aaagacctcc ggcaaggtcg atctccagga ggccgacttc atcatttgcg gaggaagggg
     1021 catgaaggcc gccgagaact ttgccatgct cgatgaaata gccgacctca tgggcggccg
     1081 cacgggagcg tcgcggacgg tcatcgacag caaatggaga gactacgacg accagatcgg
     1141 aaagagcggg aagacggtct ctccgaaact gtacatcgga tgcggcctgt caggcgccct
     1201 ccaccacatc atgggcatgg acacctcaaa ggtcatcgtg gccatcaaca aggaccccaa
     1261 tgcccccata ttccagtacg ccgattacgg catcgtggac gatgtcttcg ccatccttcc
     1321 cgccctgaag gatgaactga agaagacacg ggaagagacg taaggggcat ggacaagatc
     1381 gatgtcgtga tcgtcggggc cggcctcgcc ggcctgtcct gcgcctgggt gctttcgggg
     1441 cacggtctgc aggtcatcgt cgtggagcgc ggcgacttcg cgggcagcaa gaacgtgacg
     1501 ggcggcaggc tctacctcaa acctgtaagc ggcatggcat cggagatgct cgacggagcg
     1561 ccttttgagc gcaaggtcgt gcgcgaacgc tggagccttc tgggaagcac caattccgtc
     1621 agtatcgacc acacctcaga ccggttcagg gatgaggacc acagctacac ggtgctcagg
     1681 gcccgcctcg acaggtggct ttcggagcgc cttatggcga ggggcgtttt cgtggtcccc
     1741 aaataccgcg tcgacgatct cctgtgggag aacggagcgg tggccgggat acgcgccggg
     1801 gcggaagaga tccccgcgca cgtcgtcgtg gccgccgatg gcgtcctctc cttcatggga
     1861 cgcaaggcgg ggttgatgcc tcccctttct ccgaagacgt gtgccgtggg aatgaaagag
     1921 gtgctcgagc tttccgaaga gaagataaac gaccgtttca acgtcggtga cggggaaggc
     1981 gttgcccatc tgttcctcgg tgatgtgacg aagggtgtct tcgggggcgg ttttctctat
     2041 acgaacaagg ataccgtttc gctgggtgtg gtggcgggta tcggcgccat gatgggaagg
     2101 attccggcga tggatgcgtc gacgctcctc gatatgttca aggagcgcta cgagatgcga
     2161 agactcctcg agggcgcgaa gatagtcgag tattcggcgc atgtcatacc cgaggccggc
     2221 tatgacggca tcgggaagct ctgcggcaac ggcattctcc tcgctggcga cgccgcgggt
     2281 ctcgccctca acatgggtgt gacggtccgg gggatggaat tcgccattgc ctccggcatg
     2341 gccgccgccg aggccatagt cgaggcgaag gcgtccggtg acctttcgga aaagggcctt
     2401 tcccgctacg gggaacgtct caaggagacc ttcgtcctca aggacctcga ggcatcgagg
     2461 aacatgccgg gatacatcga caacggctca ttcttctcct actatcccga gaccttcccc
     2521 gggctcctcg agaagatcat gtggtttgac gagagcccga aaggttccct gggaaagacc
     2581 ctgtggaagg agcttcgctc atcgggtatg ctgagcgtga aacgactcat cgagctttac
     2641 cgcatcagga atatttgagg tgacaggcca tgaagataga cgaaaagctt gcccttgacg
     2701 ccttcaagac ggacagggag agccacatcc tcatagacca cgatatctgc aggtccagat
     2761 gcaccctgcg acactgcctg aacatctgcc ccgggcatct gtattccttc aacgaagagg
     2821 gcaatgagat agtcgtggaa tacgcgggat gccttgaatg cggcacgtgc atggtggcct
     2881 gcaccgaagg cgccatccag tggagctacc ccagggggga ttttggcgtg cagtaccggt
     2941 acggatgaaa gccggttgca ggtttcatat ctcaggttcc aggaaaatga gctttcttcc
     3001 actcttcgcg accttgccgt gatcgaattc ctggatcggt aagccccgcg cgcgacaagc
     3061 cccgtttcct gtctccttaa gccttaaata tgtactctcc ctagttgtac tgtctaatta
     3121 ttttcttgac aactgttctg tactgtataa gtatgtaaat tgctggttag tttacggtgt
     3181 aaacgggggg aaagtggatg agtgagtcca acagccgcaa atggatagac agtaaggagc
     3241 ttctgaccag gtcagcgata tcaagggcaa ccctcaacaa ctacatcaag ctcggcgttc
     3301 ttccgaggcc cgtcgtgaag agcccggcgg acagccagaa gggtacgaag aagatcggtt
     3361 attttcctga tactgtctta gacaccatca gcgagatcaa gaagctcaag gaaaaaggca
     3421 aacccatgga ggccatagcc agagaaatga ggacgtcctc cccggagtcc ggttacgagg
     3481 ggcctgagcg ccgcggttcc gtttcgcagg acgcgggaga gagggtgccc gtggaatctg
     3541 ttgcagtgcc gggcggagga ttgcggctga ccctcgatga ggtgaccaca ccggcatatc
     3601 tcttgaatta tcagtttgag atcgagtgga tcaacgaagc cgcggagcgc gagatattcg
     3661 ggaagccgat cagatcgata cgggatgtcg agttcagaaa cgtcttccgg cttttcctgg
     3721 gttgggagtt caaccatttt gtgaagaact gggaggagat cttcgcttac cacatggcct
     3781 tctataag
//
LOCUS       DAOQTR010000174         3786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27372_length_3786_cov_2.481372, whole genome shotgun sequence.
ACCESSION   DAOQTR010000174 DAOQTR010000000
VERSION     DAOQTR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27372_length_3786_cov_2.481372"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..2125
                     /gene="dnaK"
                     /locus_tag="PKN85_05270"
     CDS             206..2125
                     /gene="dnaK"
                     /locus_tag="PKN85_05270"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNT43849.1"
                     /translation="MARKVIGIDLGTTNSVVAVMEGGEPKVIINEEGSRLTPSVVAFT
                     KDGEVLVGQTAKRQAITNPENTIFSIKRFVGRRFNEVQEEIKTVPYKVVGDQDGNVRV
                     DVRGKQYTPQEISAFVLQKLRKSAEAYLGQQIDDAVITVPAYFNDSQRQATKDAGRIA
                     GLNVLRIINEPTASALAYGLDKKKDETIAVYDFGGGTFDISILEVGDNVVEVKSTNGD
                     THLGGDDIDQRVIDWIVSEFRKDQGIDLSKDRMALQRLKEAAERAKVELSATVETEIN
                     LPFITADASGPKHLNMKLRRAELEKLADDLFQRTIEPCRRALDDAKIPTNKIDEVVLV
                     GGSTRIPRVQDIVKEFFGKEPHRGVNPDEVVALGAAVQAGVLAGEVKDVLLLDVTPLS
                     LGIETLGGVLTRIIERNTTIPTKKSQVFTTAEDSQTSVEIHVLQGEREMARDNRTLGR
                     FHLIGLPPAPRGIPQIEVTFDIDANGILHVSAKDTATGKEQSIRITASTGLSEEDIQK
                     MVHDSETHAEEDRKRKQEIEQRNQLDNIIYTTEKTLNENKEKLSAEDIRTVEDLLNPA
                     KEAIKSGDADRIKKAADDLTKASHQLAETLYKKTSQATSQGAPGQDAGPQRDAQQSGR
                     RDDDVVDAEFEDVKR"
     gene            2135..>3786
                     /gene="lon"
                     /locus_tag="PKN85_05275"
     CDS             2135..>3786
                     /gene="lon"
                     /locus_tag="PKN85_05275"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNT43850.1"
                     /translation="MVIGLKNDKSIKDRMFIPAELPVLPLRGTVAYPDLVMPLIVGRE
                     KSIRLVDEAMSKDKMIAIITQKNPDIEDPGPEDLYTVGTVATIMKMVKMVDGSQRVVI
                     QGLCRCKVIEFTEADPNIKASILPVFEDYQKDIEIDAMYINLKNLYRKAIEMAPYLSS
                     ELAQIASKVENPGNLADLIASTINISVIEKQEILEKIDLKERLKKATIYLNREVETLE
                     LSSKIQSHVKEGIDKTQREYYLREQLKAIQKELGESDDKINEVDELKKKIKEAKMPDE
                     VRKVADKELDRLSKMSSMSAEYTVARTYLDWLTELPWSKATEDNLDIDGATKILDEDH
                     YDLEKVKKRILEYLAVRKLKADMKGPILCFVGPPGVGKTSLGKSIARALGRKFMRISL
                     GGIRDEAEIRGHRRTYVGALPGRIVQGIKKAGSNNPIFMLDEVDKIGTDFRGDPSSAL
                     LEVLDPEQNFSFSDHYLEVPFDLSKVMFIATANMLDPIPPALKDRMEVLELPGYTEEE
                     KLMIARQFLIPKERSEHGLSEDHIEFEEEALKVVIRSYTRESG"
BASE COUNT         1023 a         1083 c         1102 g          578 t
ORIGIN      
        1 ggtgcccgcc tgaggggccg caatgcctcc cgccggcctc agggcatggc ctgaaccgcc
       61 ggcgaccttc attctcgtct cccgccgata actcgccgaa ttttcagccg gatagcgtca
      121 aaaaaagatt ggtcttcaac gttgacaacg ttagacccca accttatatt acgctagacg
      181 cgaaaaatct caggaggaga cgaaaatggc aagaaaagtt ataggtatag acctgggcac
      241 caccaattcg gtcgttgcgg tcatggaggg gggcgaaccg aaggtcatca taaatgaaga
      301 aggaagcagg ctcacgccaa gcgtggtcgc gttcacgaag gacggcgagg tgctcgtcgg
      361 gcagacggcg aaaagacagg ccatcacgaa ccccgagaac accatattct cgataaagag
      421 attcgtggga agacggttca acgaggtgca ggaagagatc aagaccgttc cctacaaggt
      481 ggtgggagat caggacggca acgtgcgtgt cgatgtccgc ggcaaacagt acacccccca
      541 ggagatatcc gccttcgtgc tccagaaact gaggaaatca gcagaggcgt atctcggcca
      601 gcagatcgac gacgcggtca taaccgtgcc ggcctatttc aacgactcgc agcggcaggc
      661 cacgaaggac gccggaagga tagcgggact caacgtcctt cgcatcatca acgaacccac
      721 ggcttcagcg ctggcctacg gcctggataa gaaaaaggat gagaccatcg ccgtctacga
      781 cttcggcggc ggcaccttcg acatatccat cctcgaggtc ggcgacaacg tcgtcgaggt
      841 caaatcaacg aatggcgaca cccatcttgg aggcgacgac atcgaccagc gggtcataga
      901 ctggatcgtc tcggaattca gaaaagacca gggcatagac ctctcgaaag acaggatggc
      961 cctgcagagg ctgaaagagg cggcagagcg agcaaaggtg gagctttccg cgacagtgga
     1021 gacggagatc aatctcccct tcatcacggc tgacgcctcg ggcccgaaac acctcaacat
     1081 gaagctcagg agggcggagc tcgagaagct cgccgacgac ctcttccaga ggaccatcga
     1141 gccctgcagg cgcgcccttg atgacgcgaa gatcccgaca aacaagatcg acgaggtcgt
     1201 ccttgtcggt ggttccacca ggatccccag agtccaggat atagtcaagg aattcttcgg
     1261 caaggaacct caccggggtg tgaacccgga cgaggtcgtg gcccttggcg ccgccgtcca
     1321 ggcgggcgtt ctggccggcg aggtcaagga cgtccttctt ctcgacgtca caccgctgtc
     1381 actgggcatc gagacgctcg gcggagtact gacgaggatc atcgaacgca acaccaccat
     1441 cccgaccaag aagagccagg tattcaccac cgccgaggac agccagacga gcgtcgagat
     1501 ccacgttctt cagggcgaga gggaaatggc cagggacaac aggacgctgg ggaggttcca
     1561 cctcatcggc ctgccgccgg cgccgagggg cataccccag atagaggtca ccttcgatat
     1621 agacgccaac ggcatactcc acgtatcggc aaaggacacg gcgacgggaa aagagcagag
     1681 catccgcatc accgcctcca cgggcctctc cgaagaggac atccagaaga tggtccacga
     1741 ttccgagacc catgccgagg aggaccgcaa gagaaaacag gagatcgagc agcgcaacca
     1801 gctcgacaac atcatataca cgaccgagaa aacactcaat gaaaacaagg aaaaactctc
     1861 cgccgaggac atcaggaccg tcgaggacct gctgaacccc gcgaaggaag ccatcaagag
     1921 cggtgacgcc gacaggatca agaaggccgc ggacgacctg accaaggcct cccaccagct
     1981 tgccgaaacg ctctacaaga agacctcgca ggcaacgtcg cagggcgctc cgggccagga
     2041 tgcaggccca caacgtgacg cccagcagag cggcagaaga gatgacgacg tcgtggacgc
     2101 ggaatttgag gatgtcaagc gataggggga attgatggta atcggcttaa agaacgacaa
     2161 aagcataaaa gacaggatgt tcataccggc ggaactgccc gttctgccgc tgaggggtac
     2221 ggtggcgtac cccgatcttg tcatgcccct catcgtgggg cgcgagaaat cgatacgcct
     2281 cgtcgatgaa gcgatgagca aggacaagat gatagccatc ataacgcaga agaatcccga
     2341 tatcgaggat cccggcccgg aagacctcta taccgtcggg accgtcgcca ccatcatgaa
     2401 gatggtcaaa atggtcgacg gcagccagag ggtcgtcatt cagggtctgt gcaggtgcaa
     2461 ggtaatagag ttcactgagg cggatcccaa catcaaggca agcatcctcc ctgtcttcga
     2521 ggactaccag aaggacatcg agatagatgc catgtacatc aacctcaaga acctctaccg
     2581 caaggcgatc gaaatggccc cgtacctttc atcggagctt gcccagatag cgtcgaaggt
     2641 ggagaacccg ggcaacctgg ccgacctcat cgcctcgacg ataaacatat cggtcatcga
     2701 aaagcaggag atcctggaaa agatagacct caaggagcgc ctgaagaagg cgacgatcta
     2761 cctcaacagg gaagtggaga cgctggagct cagcagcaag atccagtcac acgtcaagga
     2821 gggcatcgac aagacccagc gcgagtacta cctgagggaa cagctcaaag ccatccagaa
     2881 ggagcttggc gaaagtgacg acaagataaa cgaggtcgat gagctcaaga agaagataaa
     2941 agaagccaaa atgcccgatg aggtgcggaa ggtggcggac aaggaactcg acaggctctc
     3001 gaagatgagc agcatgtccg ctgaatacac cgtcgcgcgg acatacctcg actggctgac
     3061 ggagttgccc tggtcgaagg cgacagagga caacctcgac attgacgggg caacgaaaat
     3121 actcgacgaa gaccactacg atctggaaaa ggtcaagaag aggatcctcg agtacctggc
     3181 cgtgaggaag ctcaaagccg acatgaaggg acctatcctg tgcttcgtcg gtcctcccgg
     3241 tgtcggcaag acgtccctgg gcaagtccat cgccagggcg ctgggccgca agttcatgag
     3301 gatatcgctc ggaggcatac gggacgaggc cgagatacgg gggcacaggc gcacgtacgt
     3361 cggcgccctc cccggccgga tcgtacaggg catcaagaaa gcagggtcca acaaccccat
     3421 attcatgctc gacgaggtcg acaagatcgg cacggacttc aggggcgacc cttcgagcgc
     3481 ccttctcgag gtcctcgacc cggaacagaa cttctccttc agcgaccact atctcgaagt
     3541 acccttcgac ctgtccaaag taatgttcat agcgacggcg aacatgctcg atcccatacc
     3601 gccggcgctg aaggacagga tggaggtcct cgagcttccc ggctacaccg aggaagagaa
     3661 gctcatgata gcacggcagt tccttatccc caaggaacgc tccgagcacg gtctctccga
     3721 agaccatatc gaattcgaag aggaagccct caaggtcgtc atacgttcct acacccgtga
     3781 atcggg
//
LOCUS       DAOQTR010000175         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27480_length_3774_cov_3.390965, whole genome shotgun sequence.
ACCESSION   DAOQTR010000175 DAOQTR010000000
VERSION     DAOQTR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27480_length_3774_cov_3.390965"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PKN85_05280"
     CDS             complement(<1..717)
                     /locus_tag="PKN85_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNT43851.1"
                     /translation="MGGFQNIGKIPELKRRIIWTLGLLAVYRVGIHIPTPGIDGNVLA
                     GIFEQAKGTLLGFFDMFAGGGLERLSIFALGIMPYISASIILQLLTVVIPTLEKLSKE
                     GEAGRRKITQYTRYGTVVISIIQGLFIAIGLEQMRGAGGEPVVFNPGWSFRLMTMITL
                     TGGTSFIMWLGEQITEKGIGNGISLIIFAGIVARMPNAVAGTWGLVNTGELNWFVVLV
                     LIAMMLAVIAFIIFMETSQRR"
     gene            complement(717..1160)
                     /gene="rplO"
                     /locus_tag="PKN85_05285"
     CDS             complement(717..1160)
                     /gene="rplO"
                     /locus_tag="PKN85_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNT43852.1"
                     /translation="MKLSDLKPVPGSKKSRKRVGRGTGSGLGTTAGYGNKGQRSTSGG
                     TKGKSFEGGQLPLTRRIPKRGFKNPFRKEYSIINIGDLEVFKGMEKVGVEDILKSGFI
                     KKVKDGIKLLAVGDIDFPIKISVHKASQKAIEKIQAKGGDVEVLV"
     gene            complement(1157..1342)
                     /gene="rpmD"
                     /locus_tag="PKN85_05290"
     CDS             complement(1157..1342)
                     /gene="rpmD"
                     /locus_tag="PKN85_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNT43853.1"
                     /translation="MSHLKIKWTKSFIGCPEDQRATVRSLGFKRLYQEKTVKNSPEIR
                     GMVKKVMHLVTVLEDAR"
     gene            complement(1344..1850)
                     /gene="rpsE"
                     /locus_tag="PKN85_05295"
     CDS             complement(1344..1850)
                     /gene="rpsE"
                     /locus_tag="PKN85_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNT43854.1"
                     /translation="MVERKRIDAGDLELQDRLVYINRVAKVVKGGRRFSFSAIVVVGD
                     GNGKVGFGLGKANEVPDAIRKAVERAKKSLIEVPVEKGTIPHEIMAKFGTSQVYMKPA
                     REGTGVIAGRAVRAVVEVAGITNILTKCYGSRNYHNVVKATIRGLSQLKSPEVALKQR
                     GKLKVEEG"
     gene            complement(1863..2228)
                     /gene="rplR"
                     /locus_tag="PKN85_05300"
     CDS             complement(1863..2228)
                     /gene="rplR"
                     /locus_tag="PKN85_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNT43855.1"
                     /translation="MARKDKVEARQRRKRRIRKKIYGTEQKPRLTVFKSIKQIYAQLV
                     DDTQHKVLTGISTLNKEVQAGLKSGGNSDAAKKVGEAIGKKALGLGITEVVFDRNGFK
                     YHGRIKALADGAREAGLKF"
     gene            complement(2240..2776)
                     /gene="rplF"
                     /locus_tag="PKN85_05305"
     CDS             complement(2240..2776)
                     /gene="rplF"
                     /locus_tag="PKN85_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNT43856.1"
                     /translation="MSRIGKKPIILPQGTQLELKDGEVLVTGPKGSLQRPFLKGLTLG
                     IDGSTVTVSRTSEEKTIRGYHGLMRTLIANMVEGVSKGFEKKLEIIGIGYRAEFQSGS
                     FTFYLGYSHPIVFALPEGITGGLEKQTQVSVQGIDKELVGQVAAKIRALRKPDAYKNK
                     GIKYADEVLRKKAGKSGK"
     gene            complement(2786..3181)
                     /gene="rpsH"
                     /locus_tag="PKN85_05310"
     CDS             complement(2786..3181)
                     /gene="rpsH"
                     /locus_tag="PKN85_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNT43857.1"
                     /translation="MVDPIADMMTRIRNAITARHETVDIPYSNMKFSISKILKEEGYV
                     RNYKTFVDEGRKKFLKVYISYDENSKSVITGLKTISKPGRRVYAKVADMPRFRNRTGI
                     VVISTSKGLMTDKNAIKNKVGGEPLLVVW"
     gene            complement(3197..3382)
                     /locus_tag="PKN85_05315"
     CDS             complement(3197..3382)
                     /locus_tag="PKN85_05315"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNT43858.1"
                     /translation="MARKAMIEKTKRAPKFKVRVRNRCAVCGRPRAFIRKFQMCRVCF
                     RSYSLRGEVPGVLKSSW"
     gene            complement(3394..>3774)
                     /gene="rplE"
                     /locus_tag="PKN85_05320"
     CDS             complement(3394..>3774)
                     /gene="rplE"
                     /locus_tag="PKN85_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNT43859.1"
                     /translation="SGDIKLITGQKPVITKAKKSIASFKLREGMSVGCMVTLRRERMY
                     EFYRKLVTIVLPRVRDFKGVSPKSFDGRGNYTLGLKEQIIFPEIDYDKIDKVRGMNIT
                     ITTTAKTDEEGHELLKLMGMPFRS"
BASE COUNT          697 a         1164 c          928 g          985 t
ORIGIN      
        1 cctgcgctgc gatgtctcca tgaatattat gaaggcaata acagcaagca tcatcgcgat
       61 gagaacaagc accacgaacc agttgagctc gccggtgttg acaaggcccc acgtaccggc
      121 gacggcgttc ggcatacgcg ccacgatacc cgcgaagatg atcagcgaga tcccgttgcc
      181 tatacccttc tcggtgatct gttccccgag ccacatgatg aaggacgtgc cgcccgtgag
      241 cgtgatcatc gtcatcagcc tgaagctcca cccggggttg aaaacgacgg gctcgccgcc
      301 ggcgccgcgc atctgttcaa ggccgatggc aatgaaaagg ccctggatga tgctgataac
      361 gacggtccca taccgcgtgt actgggtaat cttcctccgt cccgcctcac cttccttcga
      421 gagcttctcg agggtgggta tgacgacggt caggagttga agtatgattg acgccgaaat
      481 gtagggcatg atcccgaggg cgaagatgga gagcctctcc agtccgccgc cggcgaacat
      541 gtcgaaaaac ccgagcaggg ttcccttggc ctgctcaaag atgccagcca ggacattccc
      601 gtcaataccc ggcgtgggga tgtgtatgcc gacacggtag accgcaagca gaccgagggt
      661 ccatatgatc ctgcgcttca gctcggggat ctttccaata ttttggaaac ctcccattag
      721 acgagcacct ccacgtcacc acccttggcc tgtatctttt cgatggcctt ctgtgatgcc
      781 ttgtgaaccg atatctttat ggggaaatct atgtctccca cggcgagaag cttgatgccg
      841 tccttcacct tcttgatgaa acccgatttc aggatatcct caacgccgac cttttccatg
      901 cccttgaaga cctcgaggtc gccgatgttg atgatggagt attccttgcg aaaggggttc
      961 ttgaaacctc tcttggggat tcttctcgtc agcggcagct gaccgccctc gaagctctta
     1021 cccttcgttc cgccgctcgt cgagcgctgg cccttgttgc cgtagcccgc cgtcgttccg
     1081 agccccgatc cggtcccccg gccgacacgc ttgcggctct tttttgatcc cgggactggt
     1141 ttaagatctg atagtttcac cgagcatcct ccagtactgt aacaaggtgc atcacctttt
     1201 tgaccatgcc ccgtatctcc ggggagttct ttaccgtctt ttcctggtag agccttttga
     1261 aacccaggct tctcaccgtc gccctctggt cttccgggca cccgatgaag ctcttcgtcc
     1321 attttatctt gaggtggctc atcctacccc tcctcgactt tcaatttgcc cctctgtttc
     1381 agcgccacct cgggcgactt caactgggag aggcccctga tcgttgcctt caccacgtta
     1441 tggtagttcc tggaaccata acatttcgta aggatattgg tgatccccgc cacctcgacg
     1501 acggcccgaa cggcgcggcc ggcgatgacg cccgtgcctt cccgtgcagg cttcatatac
     1561 acctggctcg tcccgaactt cgccatgatc tcgtggggga tcgtcccctt ttccacgggg
     1621 acttctataa ggctcttctt tgccctttca acggcttttc gtattgcatc aggcacttcg
     1681 ttggccttgc ccaatccgaa gccgaccttt ccattaccgt cgccgacgac aacgatggcg
     1741 ctgaaagaaa acctgcggcc acccttgaca accttcgcga cacggttgat gtagacgagc
     1801 ctgtcctgca actcgagatc gcctgcgtct attcgctttc tttcaaccaa cacaacctcc
     1861 cgttaaaatt ttagcccggc ttcacgcgcc ccgtcggcga gcgccttgat ccttccatgg
     1921 tatttgaagc cgttgcggtc gaaaaccacc tctgtgatgc cgagcccgag ggccttcttt
     1981 ccgatagctt cgccgacctt ctttgcggcg tcactgttgc cgccgctttt cagtccggcc
     2041 tgcacttcct tgttaagggt cgagatcccg gtgaggacct tatgctgggt gtcatccacg
     2101 agctgcgcgt atatctgctt gatgctcttg aacaccgtga gtctcggttt ctgctcggtg
     2161 ccgtagatct tcttgcgtat tctccgtttc cgtctctgcc gggcttcgac tttatccttt
     2221 ctggccatac cgctactcct tatttcccgc ttttgcctgc tttctttctc aggacttcgt
     2281 cggcgtactt gatgcctttg ttcttgtaag catcgggttt tctcaaagcg cgtatctttg
     2341 cggcaacctg tcccaccagt tccttgtcga tcccctggac ggacacctgc gtctgctttt
     2401 caagcccgcc ggtgatgcct tcggggagcg caaagacgat gggatgtgaa taaccgagat
     2461 aaaaggtgaa acttccggac tggaattccg ccctgtagcc gatgccgatg atctcgagct
     2521 tcttctcgaa tcccttcgat acgccctcca ccatgttggc gatgagcgtc ctcatcaggc
     2581 cgtggtaccc cctgatcgtc ttttcttcgc tcgtgcggga cacggtcacg gtactaccgt
     2641 cgatgcccag cgtcaatccc ttcaggaagg gtctctggag ggagcccttg ggccccgtca
     2701 caaggacctc accgtccttg agctccagtt gtgttccctg cgggagtata attggctttt
     2761 tccctatcct cgacatacct gcacctcacc acaccacgag gaggggctct cccccaacct
     2821 tgttctttat cgcgttcttg tccgtcatga gacctttcga cgtggagatg acgacgatac
     2881 ccgttctgtt gcggaacctg ggcatgtcgg caaccttggc gtagacgcgc ctgccaggct
     2941 tgctgatcgt cttaagaccg gtgatgacgc tcttcgaatt ctcatcgtag ctgatgtaga
     3001 ccttgaggaa cttctttctg ccctcatcga caaaggtctt gtaattcctc acatagccct
     3061 cttccttgag gatcttggaa atggagaact tcatgttgga ataaggaatg tctaccgttt
     3121 catgacgagc cgtgatggcg ttgcggatcc tcgtcatcat gtctgctatt ggatcaacca
     3181 tacccatctt accacctcac cagcttgatt ttagaacacc ggggacctcg cccctcaggg
     3241 aataggacct aaagcagacc ctgcacatct ggaatttcct gataaaggct ctcggcctgc
     3301 cgcagacggc acatctgttg cgcactcgca ccttgaactt cggcgccctc tttgtctttt
     3361 ctatcattgc tttccttgcc atgcactcct ccgtcaactc ctgaaaggca tacccatgag
     3421 tttcaaaagc tcatggcctt cttcatccgt cttcgccgtt gtcgtgatcg ttatgttcat
     3481 tcccctgacc ttgtcgatct tgtcgtagtc tatctcgggg aagatgatct gctctttcaa
     3541 tcccagggtg tagttgcccc tgccgtcgaa ggacttcggg gagaccccct tgaagtctct
     3601 caccctggga aggacgatgg tcaccagttt gcggtagaac tcgtacatcc tctccctgcg
     3661 cagtgtcacc atgcatccca cggacatgcc ttcgcggagt ttgaaggagg cgatggattt
     3721 cttcgccttc gtgatgaccg gcttctggcc ggtgatcagc ttgatatcgc ccga
//
LOCUS       DAOQTR010000176         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27486_length_3774_cov_2.959129, whole genome shotgun sequence.
ACCESSION   DAOQTR010000176 DAOQTR010000000
VERSION     DAOQTR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27486_length_3774_cov_2.959129"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKN85_05325"
     CDS             <1..551
                     /locus_tag="PKN85_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNT43860.1"
                     /translation="LGYAGSCFPKDVRALINCASSKKVDLSILRAVEEANRTQRQFFL
                     DKMIGHFGEELSGRTFALWGLSFKPHTDDLREAPSLTISDALLSRGAALRVFDPVAME
                     KARTILPDDGSVTFCSDAYEALEGANALVLVTEWPQFRRPDWDKIRDLMKPAAAVFDG
                     RNQLSPAHVRALGFSYYGIGRG"
     gene            complement(760..2310)
                     /gene="citF"
                     /locus_tag="PKN85_05330"
     CDS             complement(760..2310)
                     /gene="citF"
                     /locus_tag="PKN85_05330"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HNT43861.1"
                     /translation="MAINAAGRNIPDSIPGYGEVRHFKGHDRRPPEGNRFGKRNRTAF
                     NTRTDKTLPDVKAAISASGLKSGMTISFHHHLRNGDFVVNAVLDACAEMGIKDLVIFP
                     TALFGVHKHLTKHIKSGVIRQIMGSVNGPIGQLASEGGMSVPVVLRSHGGRPRAVTSG
                     EVVIDVAFIAAPTADKYGNLSGAVGRSACGSLGYAHSDAEYAQCVVAITDNLIPYPAP
                     CIHIPQFFVDFVVSVDSIGDPAGIVSGTTRVTRDPMRLLIARNAAKLIEATPYFRDGI
                     SFQTGAGGISLAVAAYVRDAMREKGIKGSFGLGGITEFFVDMMKEGLFERLLDVQSFD
                     LAAVKSIGENPNHVEISADWYANPWNAGCAVNMLDTVVLGATEVDRDFNVNVNTEADG
                     ALLHGIGGHQDTAAGAQLTIITTPLLRGRIPCAVDSVYTVSTPGEVVDAVVTEFGVAI
                     NPRRQDLINAAKEAGDVPLVTMDELIETAEKFTGPMSPVETEDRIIGVVEYRDGTVID
                     VVRQLKKK"
     gene            complement(2310..3188)
                     /locus_tag="PKN85_05335"
     CDS             complement(2310..3188)
                     /locus_tag="PKN85_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNT43862.1"
                     /translation="MRLRRTKLYLPGNNPNMLLRGHLFNPDGVILDLEDAVAVSEKDA
                     ARILVRDVLRRGDFGSCEVTVRINAMDTEFWRTDIETVVPCGIDGIRIPKVENPKDIA
                     LMDEALSQIESKAKIRVGKTLIFCLLETALGIWRAYDIAKASRRVAAICPGGEDLRAD
                     LKTTRSPNSEELIGPRRMVVLAAHAAGVDALDTVFADITDDAGLRRESEWVKQLGYQG
                     KSVIHPNQIPIIHDVFTPTAKEIEQAKKIVEAAAEAAARGLGAVQVDGKMVDKPVVKR
                     AEFTLQRAGLKAGGEK"
     gene            complement(3185..3457)
                     /gene="citD"
                     /locus_tag="PKN85_05340"
     CDS             complement(3185..3457)
                     /gene="citD"
                     /locus_tag="PKN85_05340"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF009726.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HNT43863.1"
                     /translation="MKTAQAGTLESMDCIVTVTEAEKGRGISIVMEGASVARFGEAMV
                     KATKDTLKSLGVRDIFVSIQDNGAGDLVLKARVEAAVRRLAGGKAA"
BASE COUNT          671 a         1131 c         1076 g          896 t
ORIGIN      
        1 gtctcggcta cgccggctcc tgtttcccga aggatgtccg ggcacttatc aactgtgcgt
       61 cgtcgaagaa ggtggacctc tccattcttc gggccgtcga ggaggcgaac aggacacaga
      121 ggcagttctt cctggacaag atgatcgggc acttcggaga ggaactctcg ggaaggacct
      181 tcgctctttg gggcctgagc ttcaaacctc acaccgacga cctgagggag gcgccttcgc
      241 tgaccatatc cgatgctctt ctttcgaggg gagccgccct gagggtcttc gaccctgtgg
      301 cgatggagaa ggcccggacg atcctgcccg atgacggttc ggtgacattc tgctccgacg
      361 cctacgaggc cctggagggc gccaacgccc tggtgcttgt cactgaatgg ccccagttcc
      421 gcaggcccga ctgggacaag atcagggatc ttatgaaacc tgccgctgct gtattcgacg
      481 gccgcaacca actatctcca gcccacgtca gggccctggg cttctcctat tacgggatag
      541 ggagggggta aaaaattatt aaaggcgtga acggtaaaca gtaagcggtt aacgggatgg
      601 atcaaacaaa agcctgactg gatttttccc aggcaggaag tatcttgtgg atagtgaaca
      661 aagggaaaag gccaggagaa cacccggcct tttcctcaca gatccaaatg ttttcggctt
      721 ttgccattta ccgtttaccg ctaaccgttc accggtcttt tactttttct tcagctgcct
      781 cacgacgtcg atgacggtgc cgtcgcggta ctcgacgacc ccgatgatcc tgtcctctgt
      841 ctcgaccggt gacatggggc ctgtgaactt ctccgctgtt tcgatcagtt cgtccatggt
      901 caccaggggg acatccccgg cttcctttgc tgcgttgatc agatcctgtc tcctggggtt
      961 gatggccacg ccgaactcgg taaccacggc atcgaccact tcgccaggcg tgctgacagt
     1021 gtagaccgaa tcgaccgcgc agggtatgcg accgcggagc agaggagtgg tgatgatggt
     1081 cagctgcgct cccgctgctg tgtcctggtg gccgcctatg ccgtggagaa gagctccgtc
     1141 ggcctcggtg ttcacgttca cgttgaagtc cctgtccacc tcggtggctc ccagcactac
     1201 cgtgtcaagc atgttcacgg cgcaccccgc gttccagggg ttggcgtacc agtcggccga
     1261 tatctccacg tggttggggt tctcgcctat ggatttaacc gcggcaaggt cgaaggattg
     1321 aacgtcgaga agcctttcga agagcccctc cttcatcatg tccacgaaga actccgtaat
     1381 tcctccgagc ccgaagctcc ccttgatccc cttctcccgc atggcatccc ttacgtaggc
     1441 cgccactgca agggatatgc cacctgctcc ggtctggaag gatatgccat ccctgaagta
     1501 gggtgtcgct tcgatcagct tcgcggcgtt cctggctatc agaagcctca ttgggtccct
     1561 ggtcacccgg gtagtgccgc tgacgatccc tgcggggtca ccgatgctgt ctacagagac
     1621 aacgaagtcc acaaagaact gggggatatg gatgcagggc gcggggtagg gaatcaggtt
     1681 gtctgttatg gccacaacgc actgtgcgta ctcggcgtcg ctatgggcgt acccgaggga
     1741 cccgcaggct gatctgccta ccgctcccga aaggttgccg tacttgtccg ccgtgggcgc
     1801 tgcaatgaag gccacgtcga taacgacctc gccgcttgtc acagccctgg gacgcccccc
     1861 gtggctcctc aggacgacgg ggacgctcat gccgccctcg gaggcaagct gtcctatggg
     1921 gccgttgacg gaacccatga tctggcggat gaccccgctc ttgatgtgtt tcgtcagatg
     1981 tttgtgaacg ccgaaaagag ccgttgggaa gataacaagg tctttgatcc ccatctcggc
     2041 gcaggcgtcg agcacggcgt tgaccacgaa atcgccgttg cggaggtggt ggtggaagga
     2101 gatggtcatg ccactcttca accccgacgc gctgatggca gccttgacgt cgggaagggt
     2161 cttgtcggtc cttgtgttga aggccgttct gttcctcttc ccgaaacggt tgccctcggg
     2221 cggtcttctg tcatggccct tgaagtgcct gacctccccg taacccggta tcgagtcggg
     2281 aatattacgt cctgcggcgt tgatagccat tacttttcac ctccggcttt gagaccggcc
     2341 cgctggagag tgaattccgc cctcttgacc acgggcttgt cgaccatctt cccgtcgacc
     2401 tggaccgccc caagacccct ggccgcggct tcggcggcgg cctccacgat ctttttagcc
     2461 tgttctatct ccttggctgt cggcgtgaag acatcgtgga ttatcggtat ctggttcgga
     2521 tggatgacgc tcttgccctg gtagcccagc tgtttgaccc attcactctc ccgtctgaga
     2581 ccggcgtcgt cggtgatgtc ggcgaagacg gtgtccaggg cgtcgacacc tgcggcgtgg
     2641 gcagccagaa cgaccatcct tcggggtccg atgagctctt cgctgttggg agagcgggtc
     2701 gtcttgaggt cggccctgag gtcttcacct ccggggcaga tggcggccac ccttctggag
     2761 gccttcgcta tgtcataggc cctccagatg ccaagggccg tctctagaag gcagaagatg
     2821 agagtcttgc ctacacggat cttggccttg ctctctattt gggagagagc ctcgtccata
     2881 agggcgatat ccttcgggtt ttccaccttg ggtatgcgga tgccgtcgat accgcagggc
     2941 acgacagtct cgatgtcggt tctccagaat tcggtgtcca tggcgtttat cctcacggtg
     3001 acctcgcagc tgccgaaatc gccccttctg agaacgtccc tgaccagtat gcgcgcagcg
     3061 tccttttcgc tgaccgctac ggcatcctca aggtcaagga tgacaccatc ggggttaaaa
     3121 aggtgccccc taaggagcat attgggattg ttgccgggca ggtacagctt tgtccttctc
     3181 agtctcatgc ggccttcccc cctgcgagcc gtctcacggc cgcctcgacc ctggccttga
     3241 gcacaaggtc cccggctcca ttgtcctgga tggagacgaa aatgtcccgg acacccaggc
     3301 ttttgagggt gtcctttgtt gcctttacca tagcctcgcc gaagcgggct acgcttgccc
     3361 cctccatcac gatggatatg ccccgaccct tttctgcttc ggtcacggtg acgatacagt
     3421 ccatcgattc gagggttcct gcctgggctg ttttcatctt cttacctccc tgtgtcaagc
     3481 gacgtacttc gtcttgccgt tcatcgcttt caaaaaaccg gggcccaccc gaaggtgagc
     3541 cccgggctca ttgatctttc ctggttatgc ccttgcggtt tacgatccgc gattaagggt
     3601 tttgcaggtt ttcaggtttt gcattcccgt tcaccgttga ccgttcactg ttaacgcctt
     3661 taggttttgc cccttccgtc tcacgcctaa cgtctcaagc tcttaaggtc ttcctgccgc
     3721 ctgcgttgca tagggttttg aagtgggttt ccccataaac aaagtttttg attc
//
LOCUS       DAOQTR010000177         3742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27796_length_3742_cov_3.489829, whole genome shotgun sequence.
ACCESSION   DAOQTR010000177 DAOQTR010000000
VERSION     DAOQTR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27796_length_3742_cov_3.489829"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..603
                     /locus_tag="PKN85_05345"
     CDS             124..603
                     /locus_tag="PKN85_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43864.1"
                     /translation="MLVRYLPGRLDERIPVPVQRKIVFHGREGGNRGKLVFETTDGKR
                     QEVSLDDCSPLMSKQKKITMWLKMAGLIVLLIAFFAGGYGIALEAFSQGVAGLSVITV
                     LQLIVSGGLVVYVSLSFKAAVERCLYGGLSFDRGDIYFQSDDERAIFADILKKEERR"
     gene            605..997
                     /locus_tag="PKN85_05350"
     CDS             605..997
                     /locus_tag="PKN85_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43865.1"
                     /translation="MIPVSMKIRAGKLGSLCRFFLSAALCLLFGAVRTGDGWAQTFNE
                     SRWATETTDAGEFLSNLANIIMYGLLSISFCAGTGLIIWGFVVMANKDNNPQGAQGAW
                     KKVAFGGCLVSTSAILGIIKATMTASAG"
     gene            1004..1324
                     /locus_tag="PKN85_05355"
     CDS             1004..1324
                     /locus_tag="PKN85_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43866.1"
                     /translation="MEPGTRHDYQNINSAIIVPRLNISLEDLIICICPAMLGNGIASI
                     LSYVSEEIGVFLITTGMCGSVALYFFARGKRKEEVRQRGCSRSGVLRRYLAYLLGAEW
                     LRCR"
     gene            1328..2416
                     /locus_tag="PKN85_05360"
     CDS             1328..2416
                     /locus_tag="PKN85_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT43867.1"
                     /translation="MSNFLDDIIDRAISEDASDIYIQSESPVFLRISGRMAAAADPPE
                     GYEIDEAIERILRVSGARAQLYDREEKDLSYARQVGSGKEKKTFRFRVNLCMTKRGVS
                     IVMRRLKSVETNPLNLGVPGDLLRLVLESPTGGIIFIAGPTGSGKSTTLASLIAYLVA
                     TRCLNCVTIEDPIEYDIPEGMGNIIEREVGTTTLSFERALRAAMRQRPDVILVGEVRD
                     PETASAAIQAAKTGHLVFTTIHTLMTTQVPSRIASMFPVEKQEWMVQELTDVLLAVMA
                     QVLVPTAGTDRQRGRLVLATEFLDTKDAAIRELLVRGRYDLLRQQQRSGQIGWTLNQS
                     LRVLIDSEEVEEADARRATNDPALFMRE"
     gene            2453..2677
                     /locus_tag="PKN85_05365"
     CDS             2453..2677
                     /locus_tag="PKN85_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNT43868.1"
                     /translation="MNIHEAKTRLSELLNRVESGEGSTIARAGTPIARLVPIRDKTAE
                     RVAGTAKGKVVIEKNFDEPLPESILGEYEG"
     gene            2674..3060
                     /locus_tag="PKN85_05370"
     CDS             2674..3060
                     /locus_tag="PKN85_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNT43869.1"
                     /translation="MRLILDTHVFLWWIVDSPQPSSRVRDVMRNPVNELFLSVASAWE
                     IVIKVNLGRLRLPDRPDRFIPGQLMKNAIEPLPVEMSHALYVSRLPAIHRDPFDRTII
                     AQSILEKMPVVTRDADIAKYKTKTLW"
     gene            complement(3098..>3742)
                     /locus_tag="PKN85_05375"
     CDS             complement(3098..>3742)
                     /locus_tag="PKN85_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNT43870.1"
                     /translation="PIGRERVHYEAPEAERLDAEMGKFLTWFETAQPDPVLKAGVAHL
                     WFVTIHPFDDGNGRIARAIADMTLARSEASSQRFYSMSAQIRNERDIYYDRLEQTQQG
                     TLDITSYLEWFLACLQRALAGVDDTLGTVLRMARFWDTHASETFNLRQRLILNRLLES
                     FEGKLTSTKWAKLSKCSQDTALRDILDLVARGILVKDPGGGRSTSYSLRNDTSW"
BASE COUNT          824 a         1004 c         1155 g          759 t
ORIGIN      
        1 cctcacggcg acgccggaaa atgggcggtg atcgggaaga ccttcaaggc aaggtgcgat
       61 gtggcgatgg cgttctacaa gaagctcggc cgccaggcgg tccgggtgga ataggagttg
      121 acggtgcttg tcagatatct gccgggcaga cttgacgaaa ggatcccggt acccgttcaa
      181 aggaagatag tgtttcacgg acgcgaggga ggaaacaggg gaaagctggt cttcgagacc
      241 accgacggca agaggcaaga ggtcagcctc gatgactgca gcccgctcat gtcgaaacag
      301 aaaaagatca cgatgtggct gaagatggcg ggcctcatcg tcctgctcat agcttttttt
      361 gccggtggat acggcatcgc cctcgaagcg ttctctcagg gtgttgctgg cctcagtgtc
      421 atcaccgtac tccagctcat cgtcagcggc ggccttgtcg tgtacgtttc cctgtctttc
      481 aaggccgcag ttgagaggtg cctctacgga ggtctctcgt ttgaccgggg tgacatctat
      541 ttccagtctg acgatgaaag agcgatattc gcggacatat tgaagaagga agagaggagg
      601 taacatgatc cctgtatcaa tgaagataag ggcgggaaaa ttggggagcc tgtgcaggtt
      661 cttcctttcc gccgcattgt gcctgttatt cggcgccgtg cgtaccgggg acggatgggc
      721 gcagacattc aacgagagca ggtgggcaac ggagaccacc gatgccgggg agttcctgag
      781 caatctggcc aatataatca tgtacggtct cctgtcgatc tctttctgcg ctgggacggg
      841 tctcatcata tggggcttcg tggtgatggc gaacaaggac aacaaccccc agggtgccca
      901 gggtgcgtgg aagaaggttg ccttcggagg ctgcctcgtg tcaacctcgg cgatcctcgg
      961 gattatcaag gcgaccatga cggcaagcgc gggatagatc accatggaac ccgggacacg
     1021 tcatgactac cagaacataa acagcgccat tatcgtgccg aggctcaata tatccctgga
     1081 agacctgatt atctgcattt gccccgctat gctgggcaac ggcatcgctt caattctgtc
     1141 atacgtgtcg gaagagattg gtgttttcct catcacgacg ggcatgtgcg ggtctgtggc
     1201 cctctatttc ttcgcgcggg gaaagcgaaa ggaagaggtc aggcagaggg gctgcagccg
     1261 ctcgggcgtt ctgagaagat atctcgcata cctccttggc gccgaatggc tgcgctgcag
     1321 gtagacgatg agcaatttcc tcgacgatat catcgacagg gcgatcagtg aggacgcctc
     1381 cgacatctac atccaatcgg aaagcccggt cttcctgagg ataagcgggc gcatggccgc
     1441 tgccgccgat cctcccgaag gatacgagat agacgaagcg atagagcgta tcctcagggt
     1501 ctccggcgcc cgggcgcagc tctacgacag ggaggagaag gacctgtcct atgcgagaca
     1561 ggtgggcagc ggcaaagaga agaaaacctt caggtttcgc gtcaacctgt gcatgacgaa
     1621 aaggggggtt tccatcgtca tgaggcgcct gaagtccgtg gaaacgaacc cgctcaacct
     1681 cggcgttccg ggcgacctct tgagacttgt ccttgagagt ccgacggggg gtatcatctt
     1741 cattgccggc cccacaggca gcggcaaatc gacaacgctc gcctccctca tagcgtatct
     1801 tgtggcgaca cggtgcctca actgcgtcac catagaagac cccattgaat acgacatccc
     1861 tgaagggatg ggcaacatca tcgagcgcga ggtgggcacc acgacactgt ccttcgaacg
     1921 ggcgctgagg gcggcgatgc gccagcggcc ggacgtgatc ctggtaggtg aggtgagaga
     1981 tcccgaaaca gcgtcggcgg cgatccaagc ggccaagacg ggtcacctcg tgttcacaac
     2041 catccatacg ctcatgacca cgcaggtccc ctcgcggata gcgtccatgt tccccgtgga
     2101 gaaacaggag tggatggtgc aggaactgac ggacgttctc cttgccgtaa tggcgcaggt
     2161 cctggtaccg acggccggca cggaccgcca gcgcggccgc ctggtccttg ccacggaatt
     2221 tctggacacc aaggatgctg ccatccgcga gctcctggta cggggcagat atgacctgct
     2281 gcgtcagcag cagcgaagcg ggcagatagg ctggaccttg aaccagagcc tgagggttct
     2341 catcgattcc gaagaggttg aggaggccga cgctcgcagg gcaacgaacg atcctgcctt
     2401 gttcatgaga gagtgattag tccactactt agtctattat gggtctatga gggtgaatat
     2461 acacgaagcc aagacgagat tatcagagtt gctcaaccgg gtggaaagcg gagaggggag
     2521 cactatagcg agggcgggaa caccaatagc ccgccttgtt cccattcggg acaagaccgc
     2581 tgaacgggtg gcgggaacgg ccaaagggaa agtggtcatc gaaaagaact tcgacgaacc
     2641 attgcctgaa tcgatccttg gcgagtacga gggatgaggc ttatccttga cactcatgtg
     2701 tttctctggt ggatagtcga tagcccgcaa ccgtcatctc gcgtgcgtga tgtcatgagg
     2761 aatccggtga acgagctctt cctgagtgtg gcttccgcct gggagatagt cataaaggtc
     2821 aatctgggaa ggctccgtct gccggacaga cctgatcgat tcattccggg ccagctgatg
     2881 aagaacgcta tcgagccgct tcctgtcgag atgagccatg cgctctatgt gtcccggctt
     2941 ccggccatcc accgggatcc cttcgatcgc actatcatag cccagtcaat tctggaaaag
     3001 atgcctgttg tcacgcggga tgccgatata gccaaataca agacgaagac tctctggtga
     3061 cgggtgttaa caggaccggg acttgatcgg agacgggtca ccacgatgtg tcgttcctta
     3121 gagagtagct cgtgctgcgg ccgccgcccg ggtccttcac caggattccc cttgcgacaa
     3181 gatcgaggat atcgcgcagt gccgtatcct gggaacactt agacagcttt gcccattttg
     3241 ttgatgtcag ctttccctcg aagctctcga gcagtctgtt caggatcaga cgttgccgca
     3301 ggttgaaagt ttcgctggcg tgggtgtccc aaaaacgggc catcctcagg acggttccga
     3361 gagtgtcgtc cacgccggca agggcgcgct ggaggcaagc aaggaaccat tccaggtagg
     3421 aggtgatatc cagcgtcccc tgctgggttt gttccaacct gtcgtagtag atgtcccgct
     3481 cgttgcggat ctgggccgac atgctgtaaa aacgctgtga gcttgcctcc gagcgggcca
     3541 gggtcatatc ggcaatggca cgcgcgatgc ggccattacc atcatcgaag gggtggatgg
     3601 tgacgaacca caggtgcgcg acaccggctt tcagaacggg gtcgggctgg gcagtttcaa
     3661 accatgtgag gaacttcccc atctcagcat caagccgctc ggcttcgggc gcttcatagt
     3721 ggacgcgctc gcggccaatc gg
//
LOCUS       DAOQTR010000178         3741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27806_length_3741_cov_3.557515, whole genome shotgun sequence.
ACCESSION   DAOQTR010000178 DAOQTR010000000
VERSION     DAOQTR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27806_length_3741_cov_3.557515"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PKN85_05380"
     CDS             complement(<1..765)
                     /locus_tag="PKN85_05380"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT43871.1"
                     /translation="MRIPVLLVSFMLLFCTQVHSAAHSDSVYLFLRGYFSEALGRYDD
                     AEANYSKALREDPGSAELRTSLASLFVKKGELQKAEELLDEAIAIAPTNRVALLILAG
                     IHASKGVTYKAKALYEKLIQLNPEDTEAYMYLGSIYIGEKKYGDAITIYEKVLAYDDD
                     NIIALYYTGRLHGEAKDYEKAKSYLAKVLEIKPNFTPALLDLATIYEIEGNLEKAVEY
                     YQGVIAYDPNDKKARSRLATIFIRQKEYDKAIGKFEE"
     gene            complement(824..1453)
                     /gene="lepB"
                     /locus_tag="PKN85_05385"
     CDS             complement(824..1453)
                     /gene="lepB"
                     /locus_tag="PKN85_05385"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNT43872.1"
                     /translation="MEKKKTRTREYIESILIAALIALLVRSFVIQAYKIPSGSMEPTL
                     LVGDHLLVNRMSYVVKMPFVDNVLFTTGKPKRGDIIVFRYPEDPTKDYIKRVIATGGE
                     TVEIRNKTIFIDGKKIKDPWGHFRPEPASRGFLPFIDKDNIPPVKVPQDSYFVMGDNR
                     DNSLDSRYWGFVEKRHLVGKALIIYFSWDSGAMNALQYVRWSRIGWLIK"
     gene            complement(1514..3172)
                     /gene="argS"
                     /locus_tag="PKN85_05390"
     CDS             complement(1514..3172)
                     /gene="argS"
                     /locus_tag="PKN85_05390"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNT43873.1"
                     /translation="MLRSIAAKIINNGIEDAQRTGSIPPDLRLNFIIETPREEGFGDY
                     STNIAFILAPQLKKKPIDIARMLVERMQDSEFCESVTVAGAGFINFSLKDGLWQELLR
                     DTASKGIESLYPDIGSGKKVLIEFVSANPTGPLHIGHGRGAAVGDVLANLLEKTGHVV
                     GREYYINDAGRQIRTLGRSTFLRLRELKGETVDYPKDLYQGDYVRELAQGVIDEGLSV
                     PGGEEEAVDFLADYAGSRIMQGIKDDLGKFGVHFDNFFLESSLYASGIVDETLDILKE
                     QGIAFERDGALWFKTSLFEKDEDRVLIKSDGQNTYFTSDIAYHLDKFKRSYDVLVDIW
                     GSDHHGYIPRLKASVQALGRNKEDLRFLLIQFVTLIKEGKPVGMSTRSGEFTTLREVL
                     DEVGRDACRFFFLMRKSDAHLEFDLDLAKKTSNENPVYYIQYAHARIESIFRNAREAG
                     IDTDNLAGADCSLLIVKEELDLIRWITRYHSVLEGSARSLEPHRVTFYLMELVGRFHS
                     YYNRTRVLGSDAAVTRARLLLLSTLRGVIRDGLGLVGVSAPEKM"
     gene            complement(3305..>3741)
                     /locus_tag="PKN85_05395"
     CDS             complement(3305..>3741)
                     /locus_tag="PKN85_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNT43874.1"
                     /translation="QGDTGLPVFETSHGKFGIQMSWDNFFQEGTRILALKGADMVFAP
                     TSCAFKSQHIWQSVISSNAITNGVYVMRVNRAGSEESQDFYGMSFCVNPEGELIGGPT
                     GRSDTVLLADLDLDRLKLVRREWPILKGIKPGLYGEMLGGNL"
BASE COUNT          752 a         1062 c         1042 g          885 t
ORIGIN      
        1 ctcctcgaac ttgcctatgg ccttgtcgta ttccttctgc cgtatgaata tggtggccag
       61 cctcgagcgc gccttcttgt cgttcggatc gtaggcgatg acaccctggt agtactctac
      121 cgccttttcc aggttgccct cgatctcgta tatcgtggca aggtcgagaa gggcaggggt
      181 aaagttcggc tttatctcca ggacctttgc caggtagctc ttcgccttct cgtagtcctt
      241 ggcctcgccg tgaaggcggc ccgtatagta gagggcaatg atattgtcgt cgtcataggc
      301 aagcaccttc tcgtagatgg tgatcgcgtc gccgtatttc ttctcgccga tatagatgga
      361 gccgaggtac atataggcct cggtgtcctc ggggttgagc tgtatcagtt tctcataaag
      421 ggccttggcc ttgtaggtga cgcccttcga cgcatggatg cccgcaagta tcaggagggc
      481 cacccggttc gtgggggcga tggcgatcgc ctcatcgagg agttcctccg ctttctgaag
      541 ctcccctttc ttcacgaaga gggaggcaag ggaagtcctc agctccgcgg agccggggtc
      601 ttctctcagg gccttgctgt aattcgcctc ggcgtcatcg tacctcccca gggcctcgct
      661 gaaatacccc ctgagaaaga ggtagacgga atccgaatga gctgctgaat ggacctgggt
      721 acagaacaga agcatgaatg agacaaggag taccggtatt ctcatcaccc tgaataatac
      781 cacagaatcg atgggaaatc taatcatagg acggcatggg agcctacttt atgagccagc
      841 cgatgcgcga ccaccgaacg tactgaagag cgttcatcgc accgctgtcc caggagaagt
      901 agattatgag cgctttcccc accaggtgcc gtttttccac aaaaccccag tagcggctgt
      961 cgagactgtt gtccctgttg tcgcccatga cgaaatagct gtcctgaggc accttgacgg
     1021 gagggatgtt gtccttgtcg atgaagggca ggaaaccccg cgaggccggt tccggtctga
     1081 agtgccccca ggggtccttt atcttctttc cgtcgatgaa tatggtcttg ttccttatct
     1141 ccaccgtctc gcccccggtg gctatgacgc gcttgatata gtccttggtg gggtcctcgg
     1201 gataccggaa gacgatgatg tccccgcgct tcggtttccc cgtggtgaag aggacattgt
     1261 cgacgaaggg catcttgacc acgtagctca tcctgttgac gagcaggtgg tccccgacga
     1321 gcagggtggg ctccatggaa cccgagggta tcttgtatgc ctgaatgacg aaactcctca
     1381 cgagaagggc gatgagcgcg gctatgagga tcgattcaat gtactctctg gtcctggtct
     1441 tcttcttctc catcaaagct ccttattcat ctctccgcca ggcggtaagg cgtgtgacga
     1501 ccggggcggg cccttacatc ttctcggggg ccgacacgcc gacgagaccg aggccgtccc
     1561 tgatcacccc gcgaagggtg gatagcagga gaagacgagc tcgggtgacc gcggcatcgc
     1621 tgccaaggac tctcgtcctg ttatagtaac tgtgaaacct gcccacgagt tccatcaggt
     1681 agaaagtgac acggtggggc tcgaggctcc tggcgctgcc ttcgaggaca ctgtgatagc
     1741 gggttatcca ccgtatgagg tcaagctctt ccttcacgat aagcaggctg caatcggcgc
     1801 cggcaaggtt gtcagtgtcg atcccggcct cacgggcgtt ccggaagatg ctctctatcc
     1861 tcgcgtgggc atactggatg taatagacgg gattctcgtt ggatgtcttc ttcgcgaggt
     1921 ccagatcgaa ctcaaggtgg gcatcgctct tgcgcatgag aaagaagaaa cggcaggcat
     1981 cgcggccgac ctcgtcgagc acttccctga gggttgtgaa ctcgccggaa cgcgtcgaca
     2041 ttcccaccgg tttgccttcc tttataaggg taacgaactg tatgagaagg aatctcaggt
     2101 cttctttgtt cctccccagg gcctgaaccg atgccttcag gcgggggata tacccatggt
     2161 ggtctgaacc ccatatatcg acaaggacgt cgtaggaacg cttgaacttg tccaggtgat
     2221 aggcgatgtc ggatgtgaag taggtattct gcccgtcgga cttgatgagg accctgtcct
     2281 cgtccttctc gaaaagactg gtcttgaacc acagcgcgcc gtccctttca aaggcaattc
     2341 cttgttcctt gagtatgtcc agggtttcat cgacaatacc cgaggcatac agagacgact
     2401 cgagaaagaa attatcgaaa tgcacaccaa actttccgag atcatccttg attccctgca
     2461 ttatgcggga acccgcgtag tctgccagaa aatcgaccgc ctcctcctct ccgccgggaa
     2521 cggaaagccc ttcatcgatg accccctgcg ccagttcgcg aacgtagtcg ccctggtaaa
     2581 ggtccttggg ataatccacc gtttcgcctt taagttccct gagcctgagg aaggtggacc
     2641 ggccaagcgt tctgatctgc cggcccgcat cgttgatgta gtattccctg cccacgacat
     2701 gccctgtctt ctcgaggagg ttggcaagga cgtcccccac cgccgcgccc ctgccgtgac
     2761 ctatgtgcaa aggacccgtt ggattggcgc tcacgaactc gatgaggacc tttttcccgg
     2821 aaccgatatc gggatagaga ctttctattc ccttactggc cgtatccctg aggagttcct
     2881 gccacaggcc gtccttgagg gaaaaattaa tgaaacccgc gccggcgact gtgacggatt
     2941 cgcagaactc gctgtcctgc attctctcca caagcatcct cgcgatgtcg atgggctttt
     3001 tcttcagttg aggggcgaga atgaaggcga tgttcgtgga gtagtcgccg aacccttcct
     3061 cccggggtgt ttcgatgatg aaattcagtc ttaggtcagg gggtatcgaa ccggttcgct
     3121 gtgcgtcctc tatgccatta tttattatct ttgccgctat cgatctgagc atccactttg
     3181 tcctttttta tctgaaggtc ccgcgtgaag tcggggcatc gcagggatac gtctttcccg
     3241 tgaagatatt tcttcttgca ctcctcgcgc catgcgcaaa ccacgcagag ggtaggttcg
     3301 tcgctcacag gttgcctcca agcatctccc cgtacaatcc gggcttgatc cctttcagta
     3361 tgggccactc gcgacggacg agcttcagcc ggtcgaggtc gaggtcggcg agaaggaccg
     3421 tgtcggacct gcccgtcggt ccgccgatca actcaccctc gggattgacg cagaagctca
     3481 taccgtagaa atcctgggac tcttcactgc ccgcccggtt cacgcgcatg acatacaccc
     3541 cgttcgtgat cgcgttggaa gagatgacgc tctgccagat gtgctgggac ttgaaagcgc
     3601 acgaggtggg cgcgaagacc atatcggccc ccttgagcgc cagtattctc gtaccctcct
     3661 gaaagaaatt gtcccacgac atctgtatgc cgaacttgcc atggcttgtc tcgaagacgg
     3721 gaagtcccgt gtccccctgt g
//
LOCUS       DAOQTR010000179         3732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_27928_length_3732_cov_3.549905, whole genome shotgun sequence.
ACCESSION   DAOQTR010000179 DAOQTR010000000
VERSION     DAOQTR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27928_length_3732_cov_3.549905"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /gene="trmFO"
                     /locus_tag="PKN85_05400"
     CDS             complement(<1..1125)
                     /gene="trmFO"
                     /locus_tag="PKN85_05400"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNT43875.1"
                     /translation="MDITVIGGGLAGVEAAHQITRMGGQATLLEMRPGAGTPAHRTSH
                     LAELVCSNSLKSLDLHNAHGLLKEELRRMGSVIMDAADGTAIPGGKALVVDRDRFAAR
                     LTQTVENDPGIRLVREEALRIPEEGIVVVASGPLTSEALAEELRALTGADNLAFFDAI
                     SPIVDAASIDHGKAFYASRYMKGEAGDYLNCPLTREEYDLFHEALVSAERVDLREFEK
                     TSYFEGCLPVEVLAERGRKTLAFGPMKPVGLTDPRDGKRPYAVVQLRKENASGTMLNL
                     VGFQTKMKYAEQERVFRMIPALKNAVFLRYGSIHRNTYINAPLCLNDRLELKADRRVL
                     FAGQITGVEGYMESTAMGLAAGISAFMSATGREFCPPPPVT"
     gene            complement(1110..3353)
                     /gene="topA"
                     /locus_tag="PKN85_05405"
     CDS             complement(1110..3353)
                     /gene="topA"
                     /locus_tag="PKN85_05405"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNT43876.1"
                     /translation="MGKSLVVVESPTKMKTLSKYLGKDYVIKATYGHIKDLPKSTLGV
                     DVEEGFKPHFHVLKGKSKVVDEIKKAGKEAERILIGSDPDREGEAIAFHVAEVIGNDD
                     RIERVLFHEITKKGVLDAMGSPIKLDPAKYNAQKARRILDRLVGYKISPLLWERVSYG
                     LSAGRVQSVALRLVCEREAEIESFQKEEYWVIDVVLELPSGETFTATLERKAVDGSRV
                     PDDAKGAGKFKITSEDEAAAVKADIAGKEFIVTKVETKEKNISPQPSFITSRLQQEAS
                     RVLRLSPKRTMMLAQRLYEGIDMGEEGPVGLITYMRTDSVRVSMEAIAEAREFIGKGF
                     SKPYLPEKPNFFKNRKTAQDAHEAIRPTSVFFTPEKVKPYLDKELFALYDLIWKRFVS
                     SQMTRKKVETKTVDVTAGDYVFVARGTTVLFDGFTKIYEEVGEDEETVAILPEVRKGQ
                     KTVLKDTAMEQRFTNPPPRYSEATLIRTLEGKGIGRPSTYATIVSTVQDRDYVRKEKG
                     RLTPTPLGRTVNGLLSEFFPTVLDVGFTAKMEVRLDEIEDGKKDWIKSLEKFNTSFEG
                     ELQSAQKQMRSLKKEEKETDIECDKCGRKMLLRWGKSGEYLVCSGRPECKNKKNVKVE
                     TDGKIAIVESEAKGVCPKCGGKLVEKKGRFGRFLACSNYPECKHTEAFSLGFACPLEG
                     CPGKLVEKTSKMKKRFISCSEYPRCSFATNKEPTEGECPSCGAPTLFSFKKGLFCLRK
                     DCGWTSR"
     gene            complement(3408..>3732)
                     /locus_tag="PKN85_05410"
     CDS             complement(3408..>3732)
                     /locus_tag="PKN85_05410"
                     /inference="COORDINATES: protein motif:HMM:NF036806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43877.1"
                     /translation="VMAIPGSVLRNEHAGSNRLIKEGARLIDSVEDILTTCFPDVCPP
                     ERERVELDNREDRIYSLIGFEKVHADEVIERSGMETRDVMAALTRLTMKNVVAEVAGG
                     FFIRR"
BASE COUNT          621 a         1155 c         1065 g          891 t
ORIGIN      
        1 tgtaaccgga ggcggggggc aaaattccct gcccgtcgcg gacatgaacg cggagatgcc
       61 cgcggctagg cccattgccg tcgattccat gtacccctcg acccctgtta tctgcccggc
      121 gaagaggacc cgccggtcgg ccttgagctc gagcctgtcg ttcaggcaca ggggggcgtt
      181 gatgtaggtg ttccggtgga tgctgccgta tcggagaaaa acggcgttct tcagggcggg
      241 tatcatcctg aagacccgct cctgctcggc gtacttcatc ttcgtctgaa aaccgacgag
      301 gttgagcatg gtccccgagg cgttctcctt tctgagctgc accacggcgt agggacgttt
      361 tccgtcgcgt gggtccgtaa gtcccacggg tttcatgggc ccgaaggcga gtgttttcct
      421 gccccgctcc gcgaggacct ccacgggaag acaaccctcg aaataggacg tcttctcgaa
      481 ttccctgaga tcgacccttt cggcgctcac aagggcctcg tggaagaggt cgtattcctc
      541 tctcgtgagg gggcagttga gatagtcccc tgcttcgccc ttcatgtagc gggacgcgta
      601 gaaggccttg ccgtgatcga tgctggcggc gtcgactatg ggggatatgg cgtcgaagaa
      661 cgcgaggttg tccgcgcccg taagcgctct cagttcctcg gcgagggcct cgcttgtcag
      721 cggacccgac gcgacgacca cgatgccttc ttccggtatc cgaagcgcct cttcgcggac
      781 gagccggata ccgggatcgt tctcgacagt ctgcgtgagc cgtgcggcga accggtccct
      841 gtcgacgacg agcgccttgc cgccgggtat ggccgtgccg tctgctgcgt ccatgatgac
      901 ggaacccatc cggcgcagct cttccttgag gagcccgtgg gcgttgtgga ggtccaggga
      961 cttgagggag ttgctgcaga cgagctccgc caggtgtgag gtccggtggg cgggcgtgcc
     1021 ggcgccgggg cgcatctcaa gaagagttgc ctgtcccccc atccgggtga tctggtgcgc
     1081 ggcctcgaca cccgcgaggc cgcccccgat taccgtgatg tccatccgca atccttgcga
     1141 agacaaaaga gacccttctt gaaggagaac agggtcgggg cgccgcagga ggggcattca
     1201 ccctccgtcg gctccttgtt cgtggcaaaa gagcaccggg gatattccga gcagctgatg
     1261 aacctcttct tcattttcga cgtcttttcg accagcttgc cggggcaccc ttccaggggg
     1321 cacgcgaatc ccagcgagaa tgcctccgtg tgcttgcatt cggggtagtt gctgcaggcg
     1381 aggaaacggc cgaacctgcc cttcttctcc accagcttcc cgccgcattt ggggcatacc
     1441 ccctttgcct cgctctcgac tatggctatc ttgccgtcgg tttcgacctt gacgttcttc
     1501 ttgttcttgc actccggtct cccggaacag accaggtact cgccgctctt tccccaacgc
     1561 agcagcatct tcctgccgca tttgtcgcat tcgatgtcgg tttccttttc ctccttcttg
     1621 aggctcctca tctgtttctg ggcgctctgg agctcaccct cgaaggaggt gttgaacttc
     1681 tccagggact ttatccagtc cttcttgccg tcctcgatct catcgagacg gacctccatt
     1741 ttggccgtga aacccacgtc gagaacggtg gggaagaact cggacaggag cccgttgacg
     1801 gttctgccaa ggggcgtggg cgtgagcctg cccttttcct ttctcacgta atcacggtcc
     1861 tggaccgtgc tgacgattgt ggcgtatgtg gatggtcttc cgatcccttt cccctccagg
     1921 gtcctgataa gggttgcctc ggaataacgg gggggaggat tcgtgaaacg ctgttccatg
     1981 gccgtgtctt tgagcaccgt cttctgccct ttcctgacct cgggaagaat ggccacggtc
     2041 tcctcgtcct cgccgacctc ttcgtatatc ttcgtgaagc cgtcgaagag caccgttgtg
     2101 cccctggcga cgaagacata atcaccagcc gtgacgtcca ccgtctttgt ttccaccttt
     2161 ttccgggtca tctgggagga aacaaagcgc ttccagatga ggtcgtagag ggcgaagagt
     2221 tccttgtcga gatagggttt caccttctcg ggggtgaaga agaccgatgt gggccggata
     2281 gcctcatggg cgtcctgggc cgtcttcctg ttcttgaaga agttcggttt ctccggcaga
     2341 taaggtttgg agaacccctt cccgatgaac tcccgtgcct cggctattgc ctccatggag
     2401 acccttaccg agtccgtcct catgtacgtg atgagcccca cagggccttc ctcgcccatg
     2461 tcgatccctt cgtagagcct ttgggcgagc atcatggtgc gcttcggcga gaggcgaagc
     2521 acccgggaag cctcctgctg gagcctgctc gtgatgaagg agggctgggg cgagatgttc
     2581 ttctcctttg tctccacctt tgtcacgatg aattcctttc ccgcgatatc ggccttgacg
     2641 gcggcggcct catcttcgga tgttatcttg aattttcctg ctcccttcgc gtcatccgga
     2701 accctcgagc cgtcgacggc ctttcgttcc agcgtcgcgg tgaaggtctc tcccgagggg
     2761 agttcaagga cgacatcgat gacccagtac tcttcctttt ggaaactttc gatctcggcc
     2821 tccctttcgc agacgaggcg cagggccacc gattggaccc gtccggctga aagaccgtag
     2881 ctgacccgct cccacaggag cggacttatc ttgtagccca ccagccggtc gaggatccgc
     2941 cgtgccttct gggcattgta cttggccggg tcgagtttga tgggcgaccc catggcgtcg
     3001 aggaccccct tcttcgttat ctcatggaag aggacccgtt cgatgcggtc gtcattcccg
     3061 atgacctcgg caacatggaa ggctatggct tccccttcgc ggtcggggtc ggacccgatc
     3121 aggatccttt ccgcctcctt acccgccttt tttatctcgt cgaccacctt ggacttgccc
     3181 ttcagaacgt gaaagtgagg cttgaaccct tcctccacat cgacgcccag ggtgcttttc
     3241 ggaaggtcct tgatatggcc gtacgtcgcc ttgatgacgt aatcctttcc caggtacttc
     3301 gacagcgtct tcatctttgt cggcgactca acaacgacaa gcgactttcc catccatgtc
     3361 tccttatatt cttaaacccc ccgttcctcc gggggatcag cggaccctta tcttcgtatg
     3421 aagaagcccc ccgccacctc ggccacgacg ttcttcatgg tcaaccgggt gagggcggcc
     3481 atgacgtccc tggtttccat gccgcttctt tctattacct catctgcgtg cactttttca
     3541 aatcctatga gagaatatat cctgtcttca cggttgtcca gttcaacgcg ttcccgttcc
     3601 ggcggacaga cgtcgggaaa gcaggttgtg aggatgtctt caacggagtc gatcagcctc
     3661 gccccctcct tgatgagcct gttggaaccg gcgtgctcgt tcctcagaac gcttccgggg
     3721 atagccatga cc
//
LOCUS       DAOQTR010000180         3713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28135_length_3713_cov_3.116184, whole genome shotgun sequence.
ACCESSION   DAOQTR010000180 DAOQTR010000000
VERSION     DAOQTR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28135_length_3713_cov_3.116184"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..945
                     /locus_tag="PKN85_05415"
     CDS             205..945
                     /locus_tag="PKN85_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43878.1"
                     /translation="MTSAEELEAPPDAAEQLATLPSVITASEEEEQASAEATGEVGEE
                     PEEEKGLRFKEEIGPAVEKIQPEPEALSEPAEVREETEEIVPAPPRTEEPVMAAPPPP
                     PPPPPTERPVMAAPPPPPPPPPPPPPPPPPPPPVPEEPVMAPPPPPPPQEVSGDLAQA
                     FAEDVREQAEAEPDVAVIAGCRIKIEIPSHIAEQTVGAGVDQKRKVAGVLAAQTLEKN
                     PQLTASPTVDPVSIWSEILTVIMKRLEA"
     gene            complement(1159..2103)
                     /locus_tag="PKN85_05420"
     CDS             complement(1159..2103)
                     /locus_tag="PKN85_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT43879.1"
                     /translation="MATAKKTAQQWFLEAYHHHLLEQSIDKAIKAYKKCIQLDPAYAD
                     AYVNLGLIHLKREQYEKALQYFARVTQLEPDNIEAYINLGYAYEKMDRFGSAKQMYEH
                     SLKINPRHMEAFINLANIAEMQADYNGAIELWKKAIEVDPNAAQPHFFLASTYDRHDM
                     FDEAIEEYGQTLAIDPHHLKALFNLGRLYIQQGEPRKAFEPFARILELNGDNTAAWNY
                     LGYIYETLGSIEYAIFAYSNSLIINNYQEDAHYNLARLQYLQYRVSPDPVMLEGIRSR
                     LLYVTSVNPKHREAKQLLGMIQLHRTREQKETENQEMA"
     gene            complement(2425..2997)
                     /locus_tag="PKN85_05425"
     CDS             complement(2425..2997)
                     /locus_tag="PKN85_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNT43880.1"
                     /translation="MIDTFLLSFIPLFVAFDVLGVLPIYIRFTASMDTTKRTRHLRDS
                     LLTAFTISLLFVIVGNRVMTYLGISMQDFLIAGGIVIFITAIRDLIQGRSEPAGENEF
                     FGVVPLGIPLLAGPAVLATSMIIWNQYPFYYYLISLVLNIIICGAVLFFSDFILKLLG
                     KRVVEALSRVFSLIIASIAIMFIRKGIQGF"
     gene            complement(2994..3191)
                     /locus_tag="PKN85_05430"
     CDS             complement(2994..3191)
                     /locus_tag="PKN85_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43881.1"
                     /translation="MKVLVNDREIHLAPGMTVRHALIGAGLIRDVEAGKKVFDEWGNE
                     TGLDGALSENARIYVREGKPA"
     gene            complement(3172..>3713)
                     /locus_tag="PKN85_05435"
     CDS             complement(3172..>3713)
                     /locus_tag="PKN85_05435"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNT43882.1"
                     /translation="AIINGGGLRTSIKKGPVTVGSIYSVMPFNNYIVGIRLTGQQIRD
                     TLEYGLSGIESKEGRFPQVAGIAVTYSPGGEPGKRVRTIHVAGEPIDLSRRYIVATVD
                     FLAAGGDGYQVFREAIRQSPDFQILGGTIKSGNLVYNDAGRWVRDAVVSEAARQKKLA
                     PVVDGRIKAEGSGDEGPRK"
BASE COUNT          749 a         1057 c         1121 g          786 t
ORIGIN      
        1 gagaggcccg tgatggcagc accgccaccg cctccaccac ctccacctcc accgcctccg
       61 cctccaccgc ctccacctcc ggtacccgag aggcccgtga tggcagcacc tccaccgtct
      121 cctccgtctc caccggaaga gatgccggcg ccgatgtcag ttccgacggg cccgttcttt
      181 gcgggtcctt ccatgcaggg gcagatgacc tccgcagagg aattggaagc gccgcctgac
      241 gcggcggagc agctggccac gcttccctcc gtgatcaccg cgtcggaaga agaggagcag
      301 gcgtcagctg aagcaaccgg ggaagtcggg gaggaacctg aggaagagaa ggggttgagg
      361 ttcaaagagg aaatcgggcc tgcggtggaa aagatccagc cggagcccga agcattgtcc
      421 gagccggctg aggtgagaga agagaccgaa gagatcgtgc ctgctccccc tcgaaccgag
      481 gagcccgtga tggcggcacc cccgccgcct ccaccgccac cacctacgga aaggcccgtg
      541 atggcggcac cgcctccacc tccgcctcca cctccacctc cgcctccgcc tccaccacct
      601 cctccaccgg tacccgagga gcccgtgatg gcgccgcctc cacctcctcc acctcaggag
      661 gtgtcgggag atcttgctca ggcattcgcg gaagatgttc gggaacaggc agaggcagag
      721 ccggatgtgg cggtcattgc cggatgcagg atcaagatag agataccttc tcatatcgcg
      781 gaacagacgg tgggcgcggg tgttgatcaa aagagaaagg tagcaggcgt tctggccgca
      841 cagacgctgg agaagaaccc ccagctcacg gcctcgccga cggtcgaccc tgtctccatc
      901 tggagcgaga tactgacggt catcatgaag aggctggaag catagcgccg caacccgttc
      961 gggtcgcggc ggcttgaacc gcttgaccgt taaaaccgct tgaacgttgg aaacctgaac
     1021 ccccatcagt aaggaatgat gggggttttt gcctgtattc gtcccggcac ggcattacgc
     1081 gcggaccgag gacaatgatg cgtagacgcc ggaggtgaag ttctttaacg ctcgagcagt
     1141 tcaagcggct caagctgttc aagccatttc ttggttttcc gtttctttct gttccctcgt
     1201 tctatgcagc tggatcatgc cgaggagttg tttggcctcg cggtgcttgg ggttcacgga
     1261 agtgacgtag aggagcctgc ttcgtatccc ttcaagcatc accgggtcgg gactcacgcg
     1321 gtactggagg tattgcagcc gcgccaggtt atagtgggcg tcttcctggt agttgttgat
     1381 gatgagggag ttactgtacg cgaagatggc gtactcgatg cttcccagcg tctcgtagat
     1441 atatcccagg taattccacg cggccgtgtt gtcgccgttc aattccagga tgcgtgcgaa
     1501 gggctcgaag gccttcctcg gctcaccctg ttgtatatag aggcgcccga ggttgaagag
     1561 ggccttcaga tggtgggggt cgatggcaag ggtctgcccg tattcctcga tggcctcgtc
     1621 gaacatgtcg tgccggtcat acgttgaagc caggaagaaa tggggctgag cggcgttggg
     1681 atcgacctcg atcgccttct tccacaactc gatggccccg ttgtagtcgg cctgcatctc
     1741 ggcgatgttg gcaaggttga tgaaagcctc catgtgccgg ggatttatct tcagggagtg
     1801 ttcatacatc tgtttcgcgc tgccgaagcg gtccatcttc tcgtaggcat agccgaggtt
     1861 gatgtatgct tctatgttgt ccggttccag ctgggtcacg cgcgcgaaat actgcagggc
     1921 cttctcgtat tgttcccgct tgagatggat gagtccgaga ttgacatagg cgtccgcgta
     1981 ggcggggtcg agctgtatgc acttcttgta cgccttgatg gccttgtcga tggactgttc
     2041 aagcaggtgg tgatggtagg cttcaaggaa ccattgctgg gcagtcttct tcgcggtggc
     2101 cattcgatct cccgcactca cgcatgaggc aacaacacag tagcaaagcg gcgccgacgt
     2161 gtcaagacaa agaagaccgg tgtcaggtct cacggtccag gttttaaagt tcatgggaaa
     2221 gaaaaatggt tcgctcttgc gccggaccct ggaacccttg aactgtctgt aaactgtaca
     2281 gtccagacgg tacagtttaa aatcagctac aattcttgtc aaaacatctt cgatttcatc
     2341 tggaatatga cagtattgcg caaaggtgga ctatcaggtg tgtcgtggga acgattcaag
     2401 cggttcaagc cattcaagcc gtctttaaaa cccctgtatc cccttccgta tgaacatgat
     2461 ggcgatggag gctatgatca ggctgaagac acgggagagg gcctctacga cgcgtttgcc
     2521 caggagtttg aggatgaagt cggagaagaa gaggacggcc ccgcaaatga tgatgttgag
     2581 gacgagggag atgaggtagt aatagaaagg gtactggttc catatgatca tggaggtggc
     2641 gaggacggcg gggcccgcca gaaggggtat gcccagtggc acgaccccga agaactcgtt
     2701 ctccccggcc ggttcggagc ggccctggat gaggtctctg atggcggtta tgaagatgac
     2761 gatgcctccg gcaatgagga aatcctgcat ggatatcccg agataggtca tgaccctgtt
     2821 gccgacgatg acgaagagca gggatatggt gaatgccgtg agcagggagt ccctgaggtg
     2881 ccttgttctc tttgtggtgt ccatggaggc ggtgaaccgg atgtagatcg gcaggacccc
     2941 caggacgtca aaggccacga agaggggaat gaaggaaagg aggaaggtat ctatcatgcc
     3001 ggcttcccct ccctgacata tatccgggcg ttttcggaaa gcgccccgtc aaggcccgtc
     3061 tcgttgcccc attcgtcgaa gaccttcttc ccggcctcga cgtcgcggat caggcccgcc
     3121 ccgatgaggg cgtggcgtac cgtcataccc ggggcgagat ggatctccct gtcatttacg
     3181 aggaccttca tcgcctgatc cttcggcctt tatcctcccg tccaccacgg gggccagttt
     3241 cttttgccgc gccgcctccg atacgaccgc gtccctcacc catctgccgg cgtcgttgta
     3301 aacgaggttc ccgctcttaa tcgttccgcc caggatctgg aaatccggcg attgcctgat
     3361 ggcctcgcgg aagacctggt acccgtcccc tcctgccgcc aggaagtcca ctgtggccac
     3421 gatgtatcgt cgtgagaggt cgatgggttc cccggcgacg tggatcgttc tcaccctctt
     3481 gcccggttcc cctccggggg aatacgtgac ggcaataccc gccacctgcg gaaagcgtcc
     3541 ttccttcgat tcaatacccg agagcccgta ttcgagagtg tccctgatct gctgccccgt
     3601 cagccggatg cccactatgt agttgttgaa aggcatgacg gaatagatgc ttccgaccgt
     3661 gacgggaccc ttctttatac tggtacggag cccgcctccg ttgatgatgg cgg
//
LOCUS       DAOQTR010000181         3703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28238_length_3703_cov_4.240132, whole genome shotgun sequence.
ACCESSION   DAOQTR010000181 DAOQTR010000000
VERSION     DAOQTR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28238_length_3703_cov_4.240132"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PKN85_05440"
     CDS             complement(<1..716)
                     /locus_tag="PKN85_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43883.1"
                     /translation="MEDIKGMYKKIVKDNFPDTIKIDLGGQVLVYRKKVWNIFDADEK
                     CDVERGLRYGENPDQPSAMYELTNGNITLGDVRFFDVETGLVSRVTEKDMLQVGKHPG
                     KINLTDVDNALNILKFLDQKPACAIMKHNNPSGAAYGATTAEAFEKAFWCDRIAAFGG
                     AVVFTKTLDVETAKAMVPYYFEVVCAPDFEGGAIDVLKKWKNLRLMQIKEIERLSAFK
                     TRRTIDFKSLMDGGLIIQES"
     gene            complement(896..2785)
                     /locus_tag="PKN85_05445"
     CDS             complement(896..2785)
                     /locus_tag="PKN85_05445"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT43884.1"
                     /translation="MFDADQLTDKQIQDLKELSLVAKGDILTMTRLAGTGHPGGSMSS
                     LDLYLTVFSCADLTGPDADRIVISHGHTSPGAYASIARLGHMPIEEVVAFFRKTGSPF
                     EGHVVKGLPFIRWSTGNLGQGLSAGVGFAIAARMRGSSSHTYVFMGDGEQQKGQISEA
                     RRLAVKYGLANITALVDYNRLQISGSIDLVMPQNIVENYLADGWDIIEVNGHDHQEIY
                     HALKKARDVDNPVCIIANTVMGNGVPFMENKEKYHGSPVSEAEYKEAMAILGLEDKLD
                     YYREKRKGKWTFELSRDEQDLRIDAGSPFTYQESDKIDNRSAFGKALKDIGDRNIPQG
                     RPVSAFDCDLASSVKTADFAKAYPDNFFQGGIQEHNTATVAGALSTAGVLTFFADFGV
                     FGIDETYNQQRINDINASNLKTAITHVGLDVGPDGKTHQCIDYIGLARGLYRAKTIVP
                     CDPNQTDRVIRYVAAVRGNFFIGMGRNRWPVMYRNEGEPYFAGDYAFEYGKMDLMAYG
                     TDGAIITYGGLVSKALGIREKLAGKGKSFAVVNMPCVDEVDETVMAKLATLPSVVTYE
                     DHNVRTGIAPVITASLLKLGFTGTFLSFGATSYGASGETDEVLASQGLDVETVTAATL
                     KALKG"
     gene            complement(2788..3420)
                     /gene="nth"
                     /locus_tag="PKN85_05450"
     CDS             complement(2788..3420)
                     /gene="nth"
                     /locus_tag="PKN85_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNT43885.1"
                     /translation="MKKDVNSILNKLQEMHGEPRVELSYTNPLELTIATVLSAQCTDE
                     RVNRVTKDLFRKYTKAEDYLNVPEFELEEDIRPTGFYRNKAKSIKNIAGELLERFGGK
                     VPDDIDTFATVKGIGRKSANMIVGLAYSKPGVIVDTHVARVTGRLGLTANKDPDKIEA
                     DLRQVVPMPWWMAFSLLAILHGRYLCKAKKPDCPRCLLREDCNYYSMGGR"
BASE COUNT          665 a         1087 c         1039 g          912 t
ORIGIN      
        1 tgcgattcct ggatgatcag cccgccgtcc atgagggact tgaagtctat cgtccttctc
       61 gtcttgaagg cgctcaggcg ctctatttcc ttgatctgca tcagccgcag gttcttccat
      121 ttcttcagga cgtcgatggc gccgccctcg aagtccgggg cgcaaacgac ctcgaagtaa
      181 tagggcacca tcgccttcgc cgtttccaca tcgagggtct tcgtgaagac gaccgccccg
      241 ccgaaggccg ctatcctgtc gcaccagaag gccttttcga aggcctccgc cgtcgtcgcg
      301 ccgtacgccg ccccgctggg gttgttgtgt ttcatgatgg cgcaggcagg cttctgatcg
      361 agaaacttga ggatgttcag ggcattgtcc acatcggtca ggttgatctt ccccgggtgt
      421 tttcccacct ggagcatgtc cttttccgtg accctgctca cgaggcccgt ctcgacgtcg
      481 aagaaccgca cgtcaccgag ggtgatgttg ccgttcgtga gctcgtacat cgccgacggc
      541 tggtcggggt tctcgccgta ccggaggccc cgctcgacgt cgcatttctc gtcggcgtcg
      601 aatatgttcc acaccttctt cctgtagacg agcacctgtc cgccgagatc gatcttgatg
      661 gtatcgggaa aattatcctt gacgatcttc ttgtacatcc ccttaatatc ttccacgaag
      721 cacccctttt ttagttaatg accaatgacc aatgcaccaa accccaatga ccggaagatg
      781 atcgggaacc gaacaaagag ccaatagcat gattcaggct ttttattcgt ttggttaatt
      841 ggttattgat cattgtctct ttactggtta ttggaatttt gtcattggtc attgttcatc
      901 ctttcaaggc cttcaacgtt gcggctgtta ccgtttccac gtccagtccc tggctggcca
      961 ggacctcgtc cgtttcgccc gatgcgccgt aggaggtggc gccgaaggaa aggaaggttc
     1021 ccgtgaagcc gagtttgagc agggacgcgg ttatgacggg ggcgatgccg gtcctgacgt
     1081 tgtggtcctc gtaggtgacg accgagggca gggttgccag ctttgccatc acggtctcgt
     1141 cgacctcgtc cacgcagggc atattcacca ccgcgaagga cttccccttc ccggccagct
     1201 tctccctgat gcccagggcc ttcgagacaa gccctccata ggtgatgatg gcgccgtccg
     1261 tgccgtaggc catgaggtcc atcttcccgt attcgaaggc gtaatcgccg gcgaaatatg
     1321 gttcgccttc gttcctgtac atcacgggcc agcggttgcg gcccatgccg atgaagaagt
     1381 tgccccgtac ggccgccacg tagcggatga ccctgtccgt ctggttcggg tcgcagggaa
     1441 cgatcgtctt cgcgcggtag aggccgcgcg cgaggccgat gtagtcgatg cactggtgcg
     1501 tcttgccgtc gggccccacg tcgaggccca cgtgggtgat ggccgtcttc aggttcgagg
     1561 cgttgatgtc atttatgcgc tgctggttgt aggtctcgtc tatcccgaag actccgaaat
     1621 cggcgaagaa ggtgagcact cccgccgttg agagcgcccc cgcgacggtg gccgtattgt
     1681 gctcctggat gcctccctgg aagaagttat cgggataggc cttcgcgaag tcggccgtct
     1741 tgacggagct tgccaggtcg cagtcgaagg cgctcaccgg ccggccctgg gggatgttcc
     1801 tgtcgcctat gtccttgagg gcctttccaa aggcgctgcg gttgtctatc ttgtccgatt
     1861 cctggtatgt gaagggagaa ccggcatcga tcctaaggtc ctgctcatcg cgggagagct
     1921 cgaacgtcca cttccccttc ctcttttccc ggtaatagtc gagcttgtcc tcgagcccga
     1981 ggatggccat ggcctccttg tactccgcct cgctgacggg gcttccatgg tacttctcct
     2041 tgttctccat gaaggggacg ccattgccca tgaccgtgtt cgcgatgatg cagaccgggt
     2101 tgtcgacgtc gcgcgccttt ttcagggcgt ggtatatctc ctggtggtca tggccgttca
     2161 cctcaatgat gtcccaaccg tcggcaaggt agttctcgac gatgttctgg ggcatgacga
     2221 ggtcgatgct gccgcttatc tggagcctgt tgtaatccac gagcgccgtt atgttcgcaa
     2281 ggccgtactt cacggccagt cggcgcgcct cggagatctg tcccttctgc tgttcgccgt
     2341 cccccataaa gacgtacgtg tgggacgaac tgccgcgcat cctggcggca atggcgaaac
     2401 ccacaccggc ggacagaccc tgacccagat tgcccgtcga ccacctgatg aaaggaaggc
     2461 ccttcaccac gtggccctcg aaggggctgc ccgtcttcct gaagaatgcc acgacctcct
     2521 cgatgggcat atggccgagg cgggcaatgc tcgcgtacgc ccccggggac gtgtggccgt
     2581 ggcttatcac gatccggtcg gcatcgggcc cggtgaggtc agcgcatgaa aaaacggtga
     2641 ggtacaggtc aagggacgac atggaaccgc cggggtggcc cgtgccggca aggcgggtca
     2701 tagtcagaat atcgcccttc gcgactaacg aaagctcctt caaatcctgt atctgtttat
     2761 ccgtcagctg gtctgcatca aacatctcta tcttcctccc atggagtagt aattacagtc
     2821 ttcgcgcaac aggcatcggg gacaatcggg cttcttcgcc ttgcacaggt acctcccgtg
     2881 gagtatggcg aggagggaaa atgccatcca ccagggcatg ggcaccacct gtctgaggtc
     2941 cgcttcgatc ttatcggggt ccttgttcgc cgtgagcccc agtctgcccg ttacgcgcgc
     3001 cacatgggta tccacgatga cacccggttt gctgtatgct aggcccacta tcatgttggc
     3061 cgacttcctg cctatcccct tcaccgtcgc gaaggtgtct atatcgtcgg gaaccttccc
     3121 cccgaatctt tcgaggagct ccccggcgat gttctttata ctctttgcct tgttccggta
     3181 gaaacccgtg ggccgtatgt cctcttcaag ctcgaactcg ggcacattga ggtagtcttc
     3241 cgcctttgta tatttcctga aaaggtcctt cgtcaccctg ttgaccctct cgtcggtgca
     3301 ctgggccgac aggaccgtcg cgatggtgag ctccagcggg ttcgtatacg aaagctccac
     3361 ccggggctcc ccgtgcattt cctggagttt attcagaata gaattgacat cttttttcat
     3421 gacacaggat taaggacaat aaggatgtat catttatacc attttggggc ggggagtgtc
     3481 caagggaaaa gcaaggcggc ttgaatcgtt tgaatagctt gaatcgcttg agggttagaa
     3541 acgcggccag cttgcagcgt ttgatccctt cctccctcgt agacccttct tgatctttcg
     3601 ctctcaacag gctgttgata aactcatttt ctccgtcatt ccggctttag agactgtgtc
     3661 gcaattattt ttttcgtacc ggtacgaggg agtacttcat tgt
//
LOCUS       DAOQTR010000182         3698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28310_length_3698_cov_2.865221, whole genome shotgun sequence.
ACCESSION   DAOQTR010000182 DAOQTR010000000
VERSION     DAOQTR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28310_length_3698_cov_2.865221"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /gene="kaiC"
                     /locus_tag="PKN85_05455"
     CDS             complement(<1..1061)
                     /gene="kaiC"
                     /locus_tag="PKN85_05455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HNT43886.1"
                     /translation="MPRKRPVANRFELRKCPTGIQGIDEITGGGIPRGRPTLITGGPG
                     SGKTLIALEFLVKGITQFDEPGVFMAFEETAEDLATNVSSLGFDLNGLIEEKKLAVDY
                     VYIERAEIEDTGEYDLQGLFVRLGYAIDSIKAKRVVLDTIEVLFGSLPNEGILRAEIR
                     RLLRWLKEKGVTAIVTGERGSVTMTRYGLEEYVADCVIVLDHRVTDQISTRRLRVAKY
                     RGSYHGTNEYPFLIDRDGVSVLPITSLELTSEARSEWISTGVAGLDAMLDGKGYYRAS
                     DILISGGAGTGKSTLAAHFVKAACERGERALYFAFEESPKQIVRNMRSVGADLAPFMD
                     RGCLLVHAENWFPYWRAVRV"
     gene            complement(1337..2803)
                     /locus_tag="PKN85_05460"
     CDS             complement(1337..2803)
                     /locus_tag="PKN85_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43887.1"
                     /translation="MRTLQHPVLQYRWQVKETNGGPQLVYFGLRNPPNHTRSIIPVPR
                     ELADCLRSLDGRTSAKDLPSDLAGREMYTRFVEEGIIVDAREVRQPSTRDSFRQCVRC
                     VTNDYVLPGLEFDEDGLCAFCQCYELAERSGQGAIMQDGITDEDLVRISSGNTGSRFD
                     AMVLYTGGKDSTYLLWYLAKKLELRVLVASWNMPYTNDTCRENMRRAMRILPHVEFVE
                     RTLPWKMVRQAMRDQFERVGWPCLCPTVAHALFYPLAVQEGIPLVMHGVEEVQLAVMN
                     YVMTEIRSGQPREERVPNHRADTLNFLRMVTTISEPAQPYSLTADLARHMSSVSELLA
                     PVYKPLGDILARAESDPNMPLPILRRLTSNAAYGSWAKAVERMKQEMDWQMPPGQKGL
                     LHTSCRIEKVKDYCQFKRFQGAGSTFLPQAIVELGAGVYFGLLSRDDALEELQESGYY
                     NEPRVLSELTRDLGIDRGEARAAGEMSWTLGECTGCST"
     gene            3094..>3698
                     /locus_tag="PKN85_05465"
     CDS             3094..>3698
                     /locus_tag="PKN85_05465"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT43888.1"
                     /translation="MRKKEPSWKTSASGNPATRPDTRHRNEPHEVTSDWLRIAEGAAK
                     AGFYDWDIRTDHITWSEGIYHLFGLPPDAEPSFATWLATMHPDDIEPALTEIHESIAK
                     RISISNEYRIILPNGSERWINTLGSTFYDEAGKPLRHSGICIDITARKEMEEDLRESE
                     ERLRLAHKATSDVVWDWDIVHDTQRWNEAGAVVFGWTEIFEG"
BASE COUNT          808 a         1116 c         1035 g          739 t
ORIGIN      
        1 actcgcaccg cccgccagta cggaaaccaa ttctcggcgt gcacaagcag gcacccccgg
       61 tccatgaaag gcgcgaggtc tgctccaaca gatcgcatgt tcctgacgat ctgcttcgga
      121 gactcttcga aggcaaaata gagcgcccgc tcaccccgtt cgcatgcggc tttcacaaag
      181 tgggccgcca gcgtggattt cccggtcccc gccccgccgg agatcaggat gtccgaggcc
      241 ctgtaatagc ccttcccatc cagcatggcg tcgaggccgg cgacgcccgt ggagatccat
      301 tcagatcgcg cttccgaggt gagctccagg gaggtgatgg gaagcacgga gacgccgtcc
      361 ctgtcgataa gaaaaggata ttcgttggtg ccatggtacg agccacgata tttcgccacc
      421 ctgagacgac gggtggatat ctggtccgta acccggtgat caagaacgat aacacagtcc
      481 gccacgtatt cttcaagccc gtagcgggtc atggtgacac tacccctttc cccggtaaca
      541 atggcggtaa cgcccttttc cttgagccag cgcaggaggc gtcggatctc cgccctcagg
      601 atgccctcgt tgggaagaga cccgaagagc acctcgatgg tgtcgagaac gacacgcttt
      661 gcctttatgg aatcgatggc gtagccgagg cggacgaaga gtccctgcag gtcgtactcc
      721 ccggtatcct ctatctccgc gcgctctatg taaacatagt cgacggcaag tttcttttcc
      781 tcgataagac cgttcagatc gaaaccaaga gaggagacgt tcgtggcaag atcctccgcc
      841 gtctcctcga aggccatgaa gacacccggt tcatcgaact gtgtgatccc ctttacgagg
      901 aactcgagcg cgatgagcgt cttgccggaa cccggaccac ccgtgatcag tgtcggacgc
      961 cccctgggga tccctccccc ggtgatctcg tcgatcccct ggattcccgt gggacatttc
     1021 ctgagttcga agcgattcgc aactggtctc ttgcgtggca cagattaccc ctccctctgc
     1081 cggtttcacg gctgcgcaaa aagcagggat gaaaagaact ggcaaaacga tcaatacatc
     1141 cgcaatcttt cacaatgatt ctagcaggag aagaggccac gtgcaaagga attttcgctg
     1201 acacttaccg aagggcaaag tgaccgcgcg cgaagccccg cgcagggacc ccggaggtgg
     1261 gccctgcaat gaagcgtctt ctatttctac cgggagatga atgcgcgatc caggctcctg
     1321 accctgtgtc ttccgtctaa gtggagcacc cggtgcattc tcccaaggtc catgacattt
     1381 cacccgccgc ccgcgcctca ccgcgatcga taccgagatc gcgggtcagt tcggacagca
     1441 cccggggctc gttgtaatat cccgattcct gcagctcctc cagggcatca tcccgggaaa
     1501 gaagcccgaa atacacaccg gccccgagct cgacaatggc ctggggtagg aaggtgctcc
     1561 ccgccccctg gaagcgcttg aactggcaat agtctttgac cttttcgatg cggcagctgg
     1621 tgtgcaggag tcctttttgt cccggcggca tctgccagtc catctcctgc ttcatacgtt
     1681 cgacggcctt tgcccatgag ccataggcgg cattggacgt gagacggcgt aagatgggga
     1741 gcggcatgtt cggatcgctc tccgccctgg ccagaatgtc gcccaggggt ttgtaaactg
     1801 gcgccaggag ctcactgacg gaagacatat gccgcgccag gtcggcggtc aaagaatagg
     1861 gctgcgcggg ttcagagatc gtcgtgacca tccgcaggaa gttcaatgtg tcggcgcgat
     1921 ggttggggac ccgttcctcc cggggctgcc cggatctgat ctcggtcatc acgtagttca
     1981 tgaccgccag ttgcacctct tcgaccccgt gcatgacgag agggataccc tcctgcaccg
     2041 cgagaggata gaacagcgcg tgagccaccg tcgggcaaag gcagggccag ccgacacgct
     2101 cgaactggtc acgcatggcc tgccggacca ttttccaggg cagggtacgc tcgacgaatt
     2161 ccacatgggg aagaatgcgc atggcccgac gcatattctc cctgcatgtg tcgttggtat
     2221 aaggcatgtt ccacgaggcg acgaggacac ggagctcgag cttcttcgcc agataccaaa
     2281 gcaggtacgt ggagtccttg ccgccggtgt agaggaccat ggcgtcaaag cgtgaaccgg
     2341 tgttcccgga ggagatccgg acgaggtcct cgtccgtgat gccgtcctgc attatcgcgc
     2401 cctgccccga tctttccgcc agttcgtaac actgacaaaa ggcgcacagc ccgtcttcat
     2461 caaattccag cccggggaga acgtagtcgt tggtcacgca gcgtacgcac tgccggaagc
     2521 tgtccctggt ggacggctga cggacctcgc gcgcatccac tatgatccct tcctcaacga
     2581 atcgcgtata catttcacga ccggcaaggt ctgacggcag gtccttcgcc gaggtccggc
     2641 cgtcaagcga tcgcagacaa tccgccagtt cccgggggac agggatgatg ctcctcgtat
     2701 gattcggggg gttgcgtaag ccgaaataga caagctgcgg tccaccgttc gtctccttga
     2761 cctgccatcg gtactgcaag accgggtgct gaagtgtcct catatctcac ctccctgccg
     2821 cgttgatgtt cactacctgt tcgtagcttt acgggcccct gattccctga cgagatccga
     2881 cggttcgttt cgtctctgtc agccaggata gattcggaaa gtcatgcggc ttccgtatac
     2941 agtcttatac cttccgggaa ccggggtcaa ctccattgtt atcaacaatc cacaattcct
     3001 ccgatctata ccccatcccc acccgtagtc ttcgacaacg ttttctatac taatggtctg
     3061 aaaagggtat ataatccaga aagaggttga gtcatgcgca agaaagagcc ctcttggaaa
     3121 acctccgcat ctggcaaccc agccacgcgt cccgacacca gacacaggaa cgaaccccac
     3181 gaggtaacat cggattggct caggatcgcg gaaggagccg cgaaggccgg attctacgac
     3241 tgggacatcc gtacagacca cattacatgg tcagaaggga tctatcacct cttcgggctt
     3301 cctcccgacg cggaaccatc ctttgcgaca tggctggcta caatgcaccc cgacgacatt
     3361 gagccggccc tgacagagat acacgaatcc atcgcgaaaa ggatctctat cagcaacgag
     3421 taccgtatca tcctccccaa tgggagtgaa cgctggataa acacgcttgg aagcacgttt
     3481 tatgatgagg ccggaaaacc gctgcggcac tccggcatct gtatagacat caccgctcgc
     3541 aaagagatgg aggaagatct gcgggagagc gaggagagac tgaggcttgc ccacaaagcg
     3601 acaagcgatg tcgtgtggga ctgggacatc gtccatgaca cccagagatg gaatgaggcc
     3661 ggcgctgtcg tctttggctg gacagagatc ttcgaagg
//
LOCUS       DAOQTR010000183         3696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28323_length_3696_cov_3.629497, whole genome shotgun sequence.
ACCESSION   DAOQTR010000183 DAOQTR010000000
VERSION     DAOQTR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28323_length_3696_cov_3.629497"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..573
                     /locus_tag="PKN85_05470"
     CDS             352..573
                     /locus_tag="PKN85_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43889.1"
                     /translation="MIDPAVLEHIRIEGSRLINVIEQYKRRLPSRYCLVVPDLIRIVT
                     SQSYIDDIEIEEEAKLQKTPEPKGLRLIK"
     gene            1126..3060
                     /locus_tag="PKN85_05475"
     CDS             1126..3060
                     /locus_tag="PKN85_05475"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HNT43890.1"
                     /translation="MVADFRKRFWISLAVTLPILVLSPMIQSFLGIREAVTFTGDRYL
                     LWTLSSVVFLYGGWPFLKGIFRELADKRPAMMTLIAVAIATAYVYSSIVVFGLEGKVL
                     FWELATLIDIMLLGHWIEMRSVMGASRALEELAKLMPSEAHQLMPDGSTRDVPLDQLG
                     TGDRVLIKPGEKIPVDGDVVEGQTSVNEAMLTGESRPVSKQPGDRVLGGAINGEGSVT
                     VEVKKTGEESFLSQVIDLVRQAQESKSKTQGLANRAALWLTITALGGGILTIVIWLAI
                     VRADFDFALERMVTVMVIACPHALGLAVPLVVAVSTALAAGSGLLIRNRAAFERARNI
                     QAIIFDKTGTLTQGKFGVTDTIVFRDDLEEGELLKYAASVESRSEHPIAQGIASSSGE
                     TFKVEGFKAIPGKGAEATVAGKDVKVVSPGYLRENNIPVEDERIQQLGSQGKTVVFVM
                     IDGGLKGAVALADIIRPESKQAISRLKEMGIRCMMLTGDNRLVARWVSDEIGLDEYFA
                     EVLPQEKAEKVKEVQSRRLIVAMTGDGVNDAPALAQADVGIAIGAGTDVAVETADIVL
                     VKSNPVDVVSILGLSRATYRKMVQNLIWATGYNAFALPLAAGVFYQSGIILTPAVGAI
                     LMGLSTVIVAVNARFLKIVR"
     gene            complement(3214..>3696)
                     /locus_tag="PKN85_05480"
     CDS             complement(3214..>3696)
                     /locus_tag="PKN85_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43891.1"
                     /translation="DSVAVVDAEDKVQAYGNWLGILDGTLTEEVAKGGSTFTRRLNAD
                     RVYTAPGNGKKGRKIHICRKRADSHYAGENDYQGGGLFPFEGLFGVHRHAGCGAGFPP
                     VLLLLFRGGMQCELLSTVVALDGREASRARKIPVLPLGHQTVITSEPVCKWRIIRSRG
                     "
BASE COUNT          825 a         1056 c         1085 g          730 t
ORIGIN      
        1 cttttccgat ctccccgtgc tcgccggccg cccctgcctg accgggagcc gatcccgccc
       61 acaggagggc cagcaccgcg aatatggcaa atgtctttcc ccacatagtc atctcctcgt
      121 caccctttcc cccgcggctc cgccccggag tgggagcgcg aggaacacgc gtacattgtc
      181 acacggtttt catttgttgg caagtgcctg ctcggcgaac aacgccgcct tttccccatg
      241 ccgcgcgagg cgcatggaac accgcgagat ggaagcaacc ttaactcctc gacgtatcgg
      301 ccgataatga tcctgattgc taacgctctt cgatcgggag aagaaggact catgatagac
      361 cccgcggtac tggaacacat tcggatcgaa ggatcgcggc ttatcaacgt catcgagcaa
      421 tacaagagac gattgccctc gcggtattgc cttgtggtgc ccgacctgat acgtatagtc
      481 acgagccaga gctacatcga tgacatagaa atagaagaag aagccaagct tcagaagacc
      541 ccggagccca aaggactcag gctgataaag tgataccagt gtcggcggcc tgccccaggg
      601 cctccttcat ctgtccgaag acgggctata cggtcacctt cccctttcaa tatgaaactc
      661 cgcaccttgt ctgccttacc gaaatagtgc cgcttctccg atcagccgcc aaacccggta
      721 cagaagcgcc cagccggaga cagcttcttg acaaatcgct tttccatgga aaggcttgat
      781 ttaggatgct tagtgtcaac ggcagagcgc gatggaggat aatgccacgg gaatccagcg
      841 gcaagacgta aaaggatccg atcatcggac ttgcgccgcg taaagcttga taacacgaac
      901 tgcgcgaacg gccaagaaca cgcgcagagg aggttttatg aaaggggatc ctgtttgctg
      961 ggtgaacgtc gacgagaaat gcggcatgaa ggccacgggg caaagatgac ggcggacaag
     1021 gacgcgggtc gaatcgacga tcgactgtcg gcacatgcaa agcatggggc tctgcggggt
     1081 cgcgatcatg gagatcacgg aggccatgga ggcaaccatg cccatatggt agccgacttc
     1141 aggaagcgct tctggatatc gctcgccgtg acgctcccca tcctggttct gtctcccatg
     1201 atccagtcct tcctcggaat aagggaagca gtcacattta ccggggaccg ctatcttctc
     1261 tggaccctct cctccgtcgt cttcctctat ggcggctggc cttttctgaa agggattttc
     1321 agggaactcg ccgataaacg gcccgccatg atgacgctca tagctgtggc tattgccacg
     1381 gcatacgttt acagcagcat cgtggtcttt ggactggagg gaaaggtcct gttctgggag
     1441 ttggccaccc tgatcgacat tatgctcctc ggccactgga tagagatgag atccgttatg
     1501 ggcgcttccc gtgccctgga agaactggca aaactgatgc cctccgaggc ccatcaactc
     1561 atgcccgacg gcagcacccg cgacgtaccc ctggaccagc tcggaacagg agatagggtc
     1621 ctgatcaagc caggtgaaaa gatccccgta gacggtgatg tggtcgaagg acaaacctcc
     1681 gtaaatgagg ccatgctcac cggcgaatcg aggcccgtct caaagcagcc gggagatagg
     1741 gtgctcgggg gagcgatcaa tggagaaggc tccgttacgg tggaagtcaa gaaaaccggt
     1801 gaggaatcct ttctctccca ggtgatagat ctcgtcagac aggcccagga gagcaagtcg
     1861 aaaactcagg gcctggccaa cagggccgcc ctctggttga cgattaccgc gttgggagga
     1921 gggatcctga ccatagtcat ctggctggcc attgttcgcg cggactttga cttcgccctc
     1981 gagcggatgg tcaccgtcat ggtgatcgcg tgccctcacg ctctcggact tgcggttccc
     2041 ctggtcgtcg ctgtctccac cgcgcttgcc gcgggcagcg ggcttctcat aaggaaccgg
     2101 gcggccttcg agagagccag aaacattcag gctattatct tcgataaaac agggaccctg
     2161 acacagggaa aattcggcgt caccgatacg atcgtcttcc gggacgacct ggaggaaggg
     2221 gaactcctga agtatgcggc ctccgtggaa tcccgttcgg agcatcccat agcgcaggga
     2281 attgcctctt cctcagggga aacattcaag gttgagggct tcaaggcgat ccccggcaag
     2341 ggagcggaag caacggtcgc cggtaaggac gtcaaggtcg tcagtcccgg gtaccttcgg
     2401 gaaaataaca ttccggtgga ggatgaaaga attcaacagt tgggctctca gggcaagacg
     2461 gtggtcttcg ttatgataga cggggggctg aagggcgccg tcgcccttgc tgatattatc
     2521 agacccgaat cgaagcaggc catatcacgg ctcaaggaaa tgggcatcag atgcatgatg
     2581 ctgaccgggg acaacaggct tgtggccagg tgggtctccg acgagatagg tctggatgag
     2641 tatttcgcgg aagtgctgcc acaagagaag gccgaaaaag tgaaagaggt ccagtcccgc
     2701 cggctgattg tcgcgatgac gggagacgga gtgaatgacg ccccggccct tgcgcaggcc
     2761 gacgtcggta tcgccatagg ggcaggaacg gacgtggcgg tcgaaacggc tgatattgtt
     2821 ctggtaaaga gcaatcccgt tgatgtcgta tccatacttg ggctttcacg ggcgacgtac
     2881 agaaagatgg tccagaatct tatctgggcc acaggataca acgccttcgc tcttcctcta
     2941 gcggccggcg tgttttacca atcaggcatt atcctgaccc ctgccgtggg ggcgatcctg
     3001 atgggtctca gcacggtgat cgttgcggtt aacgcgaggt tcctgaagat cgtcagataa
     3061 gacccttgca cgaactcctc gggaggccgg cccacaacct ctgagctgtt gctaaggccg
     3121 tggcccgccg cttcgatcac ggctcatctg cctcctcctg tccgcacagc ctgataaaga
     3181 gaaaagcgcc gcaggaatgc caggatccga ggatcatccg cgggatcgga taattcgcca
     3241 tttacagacc ggctcacttg tgataacagt ttggtgcccg agaggcaaga cagggatttt
     3301 ccgcgcgcgg gaagcttccc ggccgtcaag ggcaacgaca gttgaaagga gttcgcactg
     3361 cattccaccc ctgaagagaa gcaaaaggac cggggggaat cccgcgccgc atccggcgtg
     3421 ccggtggacc ccgaacaaac cctcgaacgg aaacaacccg ccgccctgat aatcgttctc
     3481 gccagcataa tggctatcgg cccgttttct acagatatgt atcttccggc ctttcttgcc
     3541 attgccaggt gcggtgtaga cgcggtcggc gttcaggcgg cgggtgaagg tgctgccgcc
     3601 cttggccact tcctcggtca gcgtgccgtc caggatgccc agccagttgc cgtaggcctg
     3661 caccttgtcc tcggcgtcga ccacggccac cgagtc
//
LOCUS       DAOQTR010000184         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28438_length_3686_cov_3.620215, whole genome shotgun sequence.
ACCESSION   DAOQTR010000184 DAOQTR010000000
VERSION     DAOQTR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28438_length_3686_cov_3.620215"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /gene="hemC"
                     /locus_tag="PKN85_05485"
     CDS             complement(<1..433)
                     /gene="hemC"
                     /locus_tag="PKN85_05485"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNT43892.1"
                     /translation="MKKTWIVGTRGSKLALRQTQIVVDAMREANPGHEFPIRTIRTTG
                     DTVWDKPLEQIGGKGLFVKEIEEELVRGDIDIAVHSMKDLPAELAEGLVIGAVMVRED
                     PRDAFISTRLAGISELHGGCRVGTGSMRRKAQLQRYRDGIQV"
     gene            complement(396..1700)
                     /gene="hemA"
                     /locus_tag="PKN85_05490"
     CDS             complement(396..1700)
                     /gene="hemA"
                     /locus_tag="PKN85_05490"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNT43893.1"
                     /translation="MKKRAIGILGLNHNTAPLAVREKLFIKEGSIPALLKRIREEGVP
                     ESVIVSTCNRTEIYFSGEDAEEMTEKVRRVLSEAFTAPDDWFTAYTYSFSGEEAFRHL
                     FLVASGLDSMVIGEPEILGQVKDAYRIAASARSTGFFLNKTFHKTFNVAKRIRTETRI
                     GYNPLSISSMAVELAKRIFGTLGDKKILVVGAGEMCEVALKYFQKEGISDIIITNRTY
                     RKAQMLAEEIIGKAEPFETLFDLIVDVDMVLASTGSEEPLIDPMRLQGVMKKRRNRPI
                     FFIDIAVPRDVDPAVNDMDNVYLYDIDDLKELSQKHLSDRVRESEKAQVIIDNEATRF
                     SDWLRKVDVNPLISHIMNRAEEIRAKEMKRALGRMEVSDDETVNNIDAMTRALMNKLV
                     HPYLALLKENGDPAVFDTLKKLFQFEDDDDDEKDVDSGDEGL"
     gene            complement(1703..2518)
                     /gene="ccsB"
                     /locus_tag="PKN85_05495"
     CDS             complement(1703..2518)
                     /gene="ccsB"
                     /locus_tag="PKN85_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNT43894.1"
                     /translation="MNVYLYNTALGLYLASTFIYAVSLASNRPRFEKPGYYLLAAAFV
                     VHFASTAARFFEAGYTPITNIYESLSFLALCVAGFFIYIRKAYHVEMVGSIIAPLVSL
                     IVIMSLGFPRDIRPLIPALQSAWLPVHTVLSFLGNGIFVVGFFISILYLLIEKEIKRK
                     RSFLFSDKFPSLETLDRINYRCMSLGFPFLTAGIITGSIWAGFAWGSYWRWDPKETWS
                     LITWIVYAILFHNRLALGWRGRRTAYMMILGFLLVLFTFLGVNFLLKGLHTYV"
     gene            complement(2515..3159)
                     /locus_tag="PKN85_05500"
     CDS             complement(2515..3159)
                     /locus_tag="PKN85_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNT43895.1"
                     /translation="MRSDTEKHHYYPLFLDVTGKECLVVGGGSVAERKALMLLKFNAS
                     VTVVSPKVTKKLADLADRGVLRVRTRKYRRSDLDNAAVVFACTDDRDTNGAVREDAAK
                     KGVLINVVDKPKECDFIVPSIIRKGDVTIAISTSGTLPMASKKIRQAIERTVTKDYVS
                     YTRIIGALRRHLIDTVPDPLRRKAIMKKISAMEVGDVVRRGLTGMKKVLDEGLS"
     gene            complement(3143..3418)
                     /locus_tag="PKN85_05505"
     CDS             complement(3143..3418)
                     /locus_tag="PKN85_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNT43896.1"
                     /translation="MQARAHIVVHGTVQGVFFRYSTQREAQGLGLTGWVRNLPDGSVE
                     IVCEGEKDAVQALVDWSRRGPRGAFVETADVSWEDHTGRFLTFEIRY"
BASE COUNT          714 a         1049 c         1092 g          831 t
ORIGIN      
        1 cgacctgtat cccatcgcgg tacctctgga gctgggcctt cctgcgcatg ctgcccgttc
       61 ccacacggca gccgccatga agctcgctta tgccggcgag ccgtgtcgag atgaaggcgt
      121 ccctggggtc ctcgcggacc atgacggccc cgatgacgag gccctcggca agttccgcgg
      181 gaaggtcttt catgctatgt acggcgatgt cgatgtcccc gcggacgagt tcctcttcta
      241 tctccttgac gaaaaggccc tttcctccta tctgctcgag gggtttgtcc catacggtgt
      301 cgccagtcgt cctgatggtc cttattggga attcatggcc ggggttggcc tctctcatgg
      361 catcaaccac tatctgtgtc tgcctgaggg ccagcttaga gcccctcgtc cccactatcc
      421 acgtcttttt catcgtcatc gtcgtcctcg aattgaaaga gcttcttcag cgtgtcgaac
      481 acggccggat ccccgttctc cttgagcagc gccaggtagg gatggacgag cttgttcatg
      541 agcgccctcg tcatggcgtc gatattgttc accgtctcgt cgtcgctgac ctccatcctg
      601 ccaagggccc ttttcatttc cttcgcccgt atctcttcgg cccggttcat gatgtggctg
      661 atgagggggt tgacatccac cttcctgagc cagtcagaga acctggtcgc ctcgttatct
      721 atgatgacct gcgccttttc tgactcacgg accctgtcgg agaggtgttt ctgggagagc
      781 tccttgaggt cgtcgatgtc atagaggtag acattgtcca tgtcgttgac ggcagggtcg
      841 acgtcgcggg gcacggcgat gtcgatgaag aatatgggcc tgttcctgcg ttttttcatc
      901 accccctgga gcctcatggg gtcgatgagg ggctcctcgg accccgtgga ggcgaggacc
      961 atgtcgacat cgacgatgag atcgaagagc gtctcgaagg gttcggcctt tccgatgatc
     1021 tcctcggcca gcatctgcgc ctttcgatag gtcctgttgg tgatgatgat atcggagatc
     1081 ccttccttct gaaagtattt cagggccacc tcgcacatct ccccggcgcc gacgacgagt
     1141 atcttcttgt ccccgagggt cccgaatatg cgctttgcca gctccacggc catggagctg
     1201 atggacaggg ggttgtaccc gatccgcgtc tccgtccgta tgcgctttgc gacgttgaat
     1261 gtcttgtgaa aggtcttgtt cagaaagaaa cccgtcgatc tcgccgaagc ggcaatgcgg
     1321 tacgcgtcct tcacctggcc cagtatctcc ggttcgccga tgaccatgga gtcgaggccc
     1381 gaggcgacga ggaagaggtg gcggaaggcc tcttcgccgg agaagctgta cgtgtaggcc
     1441 gtgaaccagt catcgggtgc cgtgaaggcc tcggagagca cccggcggac cttctccgtc
     1501 atctcctcgg cgtcctcgcc ggagaagtag atctccgtcc tgttgcaggt ggagacgatg
     1561 acggattccg gaacgccttc ttcgcggatc ctcttcagga gagccggtat ggatccctcc
     1621 ttgatgaaaa gcttttcccg gaccgcaagg ggcgcagtgt tgtgattgag tcccagtatg
     1681 ccgatcgccc gtttcttcat ggtcacacgt aggtatgcag gcccttgagg aggaaattga
     1741 ccccgaggaa cgtgaagagg acgagcagga aacccaggat catcatgtac gccgtcctcc
     1801 ttccccgcca cccgagagcg agcctgttgt gaaagaggat ggcgtagact atccacgtga
     1861 tgagcgacca cgtctccttg gggtcccatc tccagtagga accccaggca aaacccgccc
     1921 atatggagcc cgtgattatc cccgcggtga ggaagggaaa acccagggac atgcaccggt
     1981 agttgatcct gtccagggtc tccagggagg ggaacttatc ggaaaagagg aaggacctct
     2041 ttctcttgat ctctttttct atgaggaggt agagaatgga tatgaagaaa ccgacgacga
     2101 atatcccgtt ccccaggaat gaaaggacgg tatggacggg aagccaggcg ctttgcaggg
     2161 ccgggatgag gggccgtatg tccctcggga aacccagcga catgatgacg atgagggaga
     2221 caaggggagc gatgatgctt cccaccatct ccacgtggta ggccttcctt atatatatga
     2281 agaaaccggc tacgcacagc gccaggaagg aaagggactc gtagatgttc gtgatggggg
     2341 tgtagccggc ctcgaagaat cgcgccgccg tggaggcgaa gtggaccacg aaggcggcgg
     2401 caagaagata gtatcccggt ttctcgaagc gcggcctgtt cgaggcgagg ctcacggcat
     2461 agatgaaggt ggacgcgaga tagagcccga gtgccgtgtt gtaaaggtaa acgttcatga
     2521 gagcccttcg tcgaggacct tcttcatgcc cgtgaggccg cgcctgacga cgtcgcccac
     2581 ctccatggcg gatatcttct tcatgatggc cttgcgcctc aaggggtcgg ggacggtgtc
     2641 gatgagatgc cttcttaagg cgcctatgat gcgcgtgtag gagacgtagt ccttcgtcac
     2701 cgttctttcg atggcctgcc gtatcttctt ggaggccatg gggagggtcc ccgacgtgga
     2761 tatcgctatc gtcacgtccc cttttctgat gatggagggg acaatgaaat cgcactcttt
     2821 cggtttgtcg acgacattga tgaggacccc cttctttgcc gcgtcctcgc ggacggcgcc
     2881 gttcgtgtcc ctgtcgtcgg tgcaggcgaa cacaaccgcc gcgttgtcga ggtcgctccg
     2941 gcggtatttc cgcgtgcgaa cgcgcagcac ccccctgtcg gcgaggtctg ccagcttctt
     3001 cgtcaccttc gggctcacga cggtcaccga ggcgttgaac ttgagcagca tgagggcctt
     3061 gcgctcggcg acactcccgc cgccgacgac aaggcattcc tttcccgtca cgtcgaggaa
     3121 gaggggataa taatgatgct tttcagtatc tgatctcaaa agtaaggaac ctgccggtat
     3181 gatcttccca ggagacatcg gccgtctcga cgaaggcgcc ccggggacct ctgcgggacc
     3241 agtcgaccag cgcctggacg gcgtctttct cgccttcaca gacgatctcc acgctgccgt
     3301 cggggaggtt cctgacccag cccgtcagcc cgaggccctg ggcctcgcgt tgggtgctgt
     3361 aacgaaagaa aacgccctgc actgtgccgt gtacaactat atgtgcccgt gcctgcatgg
     3421 tgaaacaacg acctctttgg atgccgaaaa accccgccaa cgcccgtccg taaacatttt
     3481 attgttggca agaggttttt tgtattatac ttatcacaac attcccgcaa aatccagttg
     3541 tgttgaccac ttgggagaag gcgattatgt acagactggg tatagatatc ggttcggtta
     3601 gcgtcaacgt cgcggttgtc aacgagaacg ggaaggtcgt caggtctcag tatatccgtt
     3661 acaaggggaa acctttcgtc acggcg
//
LOCUS       DAOQTR010000185         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28467_length_3683_cov_4.258269, whole genome shotgun sequence.
ACCESSION   DAOQTR010000185 DAOQTR010000000
VERSION     DAOQTR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28467_length_3683_cov_4.258269"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1352)
                     /gene="ahbC"
                     /locus_tag="PKN85_05510"
     CDS             complement(168..1352)
                     /gene="ahbC"
                     /locus_tag="PKN85_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HNT43897.1"
                     /translation="MIGISKLYCGAVEASDPLRYGRDSSLLPSHLLQFSKDKRPVVVW
                     NTTKRCNLRCVHCYAFAEGEDYRGDELSTDEGKALIDDLARFRVPVILFSGGEPVLRE
                     DLPELIDHAVKKGVRAVISTNGTLITKEKARVFARCSLSYIGVSLDGLTEINDAFRGV
                     EGAFARAMEGIRNARDAGIKVGLRFTINKRNAGEVPKIFDLIEEENVQRVCFYHLVYA
                     GRGSKLIGEDLSHDETRRVVDLIMDRTKALFARGGSPEVLTVDNHADGPYVYLRLLRE
                     DPRRAAEVLELLRMNEGNSSGVGIACIDEKGNVHADQFWRHHSFGNVRERSFSTIWED
                     LADPVMAKLKEKKKYVVGRCAACRWLDVCAGNFRVRAEAATGNLWAEDPQCYLTDEEI
                     KR"
     gene            1465..1543
                     /locus_tag="PKN85_05515"
     tRNA            1465..1543
                     /locus_tag="PKN85_05515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1501..1503,aa:Pro,seq:tgg)
     gene            complement(1687..2340)
                     /locus_tag="PKN85_05520"
     CDS             complement(1687..2340)
                     /locus_tag="PKN85_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNT43898.1"
                     /translation="MDLIEAIKVRKSARAFKPDAVSRQQIEEILSLVVRAPSAINLQP
                     WEFIVVMGEEKERLSRRLVKLYREKQISCSPGNVKPLSAEFHKRGAESFILMNPYLEK
                     MGQEFNRFINEGSCNFYGAPSAVIFCLDNAFSEARLVDIGIALGYFVLIAHNFGLSTC
                     PIGLINAYEDDIKDMLNIPDNKDVVIGAALGYPDLASPINEFTSPREDLENFVRWID"
     gene            complement(2377..2952)
                     /locus_tag="PKN85_05525"
     CDS             complement(2377..2952)
                     /locus_tag="PKN85_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNT43899.1"
                     /translation="MRVHFSFERPQDHPNIKAWREAFKKMGMAPTKYPSFIESLLKRA
                     LKGGPFPRFNPIVDLYNSIALKYLVPISGHAISGVDGNISLTFAAGTETFIPMDMGEQ
                     EAVDKGEVIVKDDRDALTRRWLWKRSNKDRIESETTRACLLIHAMSGLPDWLCERAAK
                     DLVDSILVNEYGRIVHRATVTASQRETEFAV"
     gene            complement(3075..3476)
                     /locus_tag="PKN85_05530"
     CDS             complement(3075..3476)
                     /locus_tag="PKN85_05530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNT43900.1"
                     /translation="MLNLEIITPERTVVKDEVDVVEATGAEGEFGIMPGHTQFLTTLR
                     IGEIRYRKGSETTYIASSGGFAEVVDDKVTLLVDTAESAKEIDVERAKKAMDRAEALL
                     KTMAYDHTEYRMMEMALLRAIARIGVASKKI"
     gene            complement(3486..>3683)
                     /locus_tag="PKN85_05535"
     CDS             complement(3486..>3683)
                     /locus_tag="PKN85_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNT43901.1"
                     /translation="RFLSQPFFVAEVFTGQPGRYVALKDTIRGFKEIVEGKHDELPEQ
                     AFFMVGTIEEAVEKAKQFMGE"
BASE COUNT          741 a         1105 c          965 g          872 t
ORIGIN      
        1 caggcgccag tgaccacgaa ctgataatga caaatctacc agattccagc cgaccagaca
       61 acagtcacgt gcgtggccaa tgggtcgtct cgtctgtcgt ttctggctgg agattgatta
      121 ttgctcgatt ggctattgtt tggttatttg aatttgatca ttgatcatta tcgttttatc
      181 tcttcatccg tcagataaca ctgcgggtcc tcggcccaga ggttgcccgt tgccgcttcg
      241 gcgcggacgc ggaagttgcc ggcacagacg tcgagccatc tgcacgctgc gcagcggccg
      301 accacgtact tcttcttttc cttgagcttc gccatgaccg ggtccgcaag gtcctcccag
      361 atcgtgctga aagaccgctc ccggacgttg ccgaagctgt ggtgcctcca gaactggtcc
      421 gcgtggacat ttcccttttc atcgatgcag gcgatgccga cgcccgaaga gttcccttcg
      481 ttcatcctga gcagttccaa cacctccgcc gcccttcgcg ggtcctccct gagaagcctc
      541 agatacacgt atggaccgtc ggcgtggttg tcgacggtaa ggacctccgg cgatcctccc
      601 ctggcaaaga gggccttcgt cctgtccatg atgaggtcga cgacgcggcg cgtctcatca
      661 tgggagaggt cctcgccgat gagcttcgat cctcgccccg cgtacacaag gtgatagaaa
      721 cagacacgtt gaacgttctc ttcctctatg agatcgaaga tcttcggtac ctcgccggca
      781 tttctcttgt tgatcgtgaa cctgaggccc accttgatac ccgcgtccct cgcattcctg
      841 atgccctcca ttgcacgcgc gaaggcaccc tcgaccccgc ggaaggcgtc gttgatctcc
      901 gtcagaccgt cgaggctgac accgatgtag gacagcgaac accgggcgaa cacccgggcc
      961 ttctccttcg tgatgagggt accgttcgtc gatatgacgg ccctgacgcc ctttttcacc
     1021 gcgtggtcga tgagctccgg aaggtcctcc ctcagcaccg gctcccctcc ggagaagaga
     1081 atgacgggca cacggaaacg agccaggtca tcgatgagcg ctttgccctc gtccgtcgac
     1141 aactcgtcgc ccctgtaatc ttccccctcg gcaaaagcgt agcagtgaac gcacctgaga
     1201 ttacagcgct tcgttgtgtt ccacacaacg acgggtctct tgtcctttga gaactgcaga
     1261 aggtgtgacg gcaaaagcga agaatccctg ccgtacctca gggggtcgga ggcctctacg
     1321 gccccgcagt agagtttgga tatgccgatc atggtgccct tattgtgagc cgaaacgtga
     1381 aaaaagtcaa cgccgttgtc cctcaccctg taagggcccg acttattgaa atgcgacttt
     1441 ttctgtgata tgctggtggc tgatcggggc gtggcgcagt atggttcagc gcgcttgcct
     1501 tgggcgcaag aggccgctgg ttcaaatcca gtcgccccga ccagaaaacc ggcttaaacc
     1561 gcttgaaccg cttgaacgtc cgaggcgaat ggaggctttt cacgcggtgt tcggttatat
     1621 cacgcgattc tttccttttt cttaccctca aacgctcaag cggttcaagc gattcaagcc
     1681 gtctttctag tctatccacc tcacgaagtt ctcaaggtcc tcacggggag aggtgaactc
     1741 gttgataggg cttgccaggt cgggatagcc cagagcggct ccgatcacca cgtccttgtt
     1801 gtcggggatg ttgagcatgt ccttgatatc atcctcatag gcgttgataa gcccgatggg
     1861 gcaggtggaa aggccgaagt tgtgggcgat caggacaaaa tatccgaggg cgatcccgat
     1921 gtccacgaga cgggcctcgg aaaaggcgtt gtcgaggcaa aaaatgactg ccgacggcgc
     1981 gccgtagaag ttgcagctgc cctcgttgat aaaccggttg aactcctggc ccatcttctc
     2041 caggtagggg ttcatcagta tgaaggattc agctcccctc ttgtgaaact cagctgaaag
     2101 gggcttcacg ttcccgggac tgcaggagat ctgcttttca cggtagagct tcaccagtct
     2161 gcggctcagc cgttccttct cctcacccat gacaacgatg aattcccagg gctggagatt
     2221 gatggccgac ggcgcgcgga ccaccaggct cagtatctcc tctatctgct gtctagagac
     2281 cgcatcgggc ttgaaggccc gtgcgctctt cctcacttta atggcttcga tgagatccat
     2341 gggatcagtc ctcctagttg ccgaacccgc cgacactcat accgcgaatt cggtctctct
     2401 ttgtgatgcc gtcactgtcg cccgatgaac gatccttccg tactcattga cgaggatgct
     2461 atcgacaagg tccttcgctg ccctctcgca gagccagtcg ggcaggcccg acatggcatg
     2521 gatcaaaaga cacgcccttg tggtctcact ctcgatcctg tccttgttcg atcgcttcca
     2581 gagccaccgg cgggtcaggg cgtccctgtc atccttcacg atcacctcac ccttgtctac
     2641 ggcctcctgc tcgcccatat ccatggggat gaaggtttcc gtgccggcgg cgaaggtgag
     2701 gctgatattt ccgtccaccc cgctgatagc gtgcccgctg atgggaacaa ggtacttgag
     2761 ggcgatcgaa ttgtacaggt cgactatggg gttgaaacgc gggaacggtc cccccttgag
     2821 ggcccgcttg agaagggatt ctataaaact cggatacttc gtcggggcca tccccatctt
     2881 cttgaaggct tcccgccagg cttttatatt cggatggtcc tgcggacgtt caaaagaaaa
     2941 atggactctc agatcgtcaa cgacctcctc gagtccatct tcgccgtacc ttatgttgtc
     3001 aatctcgcaa acaaccacac cgacctgcaa tgcaggaaaa aggctgaata gcccctcgtc
     3061 tatggtgaag accattatat tttcttggac gccacgccta tacgggcaat cgcccggagc
     3121 aatgccattt ccatcatccg gtattccgta tgatcatagg ccatcgtctt caaaagggct
     3181 tcggcacgat ccattgcctt cttcgcccgt tcaacatcta tttccttcgc ggactccgcg
     3241 gtatctacaa gcaatgtcac cttgtcgtcg accacctcgg caaacccgcc gctcgatgca
     3301 atgtaggtgg tctccgaacc tttcctgtac cggatctctc ctatacgaag ggtcgtgagg
     3361 aactgggtgt ggcccggcat gataccgaac tcgccctctg ctcccgtggc ctccaccaca
     3421 tcgacttcgt ccttgacaac cgttctctca ggggtaatta tctcaaggtt gagcataacg
     3481 ttctcttatt ctcccatgaa ctgtttggct ttttcaacag cctcttctat ggtgcccacc
     3541 atgaagaatg cctgctcggg aagctcgtcg tgtttgcctt caacgatctc tttgaatccc
     3601 ctgatcgtat ccttcagagc aacgtaccgg ccgggttggc ccgtgaagac ctccgcaacg
     3661 aagaagggct gagagaggaa cct
//
LOCUS       DAOQTR010000186         3682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28478_length_3682_cov_2.886407, whole genome shotgun sequence.
ACCESSION   DAOQTR010000186 DAOQTR010000000
VERSION     DAOQTR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28478_length_3682_cov_2.886407"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1297
                     /gene="glyA"
                     /locus_tag="PKN85_05540"
     CDS             50..1297
                     /gene="glyA"
                     /locus_tag="PKN85_05540"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNT43902.1"
                     /translation="MKLDGKDTKVFELMRRELDREEYSLILIASENYVDEDVLATQGC
                     VLTNKYAEGYPSKRYYSGCAYLDEIESIAIERARTLFGVEHANVQPHSGSSANMAVFY
                     AVLERGDTVLGMDIAHGGHLTHGARPSFSGKWYRAVSYPVSAKNEMLDYDEIRGIARK
                     ERPKLIIAGASAYSKIIDFAKFREIADEVGAYLMADIAHIAGLVAAGLHPSPAGHAHF
                     ITTTTHKTLRGPRGGLVMCNADLAKKIDSAVFPGIQGGPLMHVIAAKAVALRQAMTEE
                     FKEYQRQIIANAKHMAKCLADLGFRIVSGGTDNHLFLVDLTPKNVTGNDAQIALEASG
                     IMVNKNLIPFDTKTPGVTSGIRIGTPAVTTRGMKEKEMEVICEMIDSVLKRPGDTSFM
                     DGTRERVREMCKGFPFYSRIYDI"
     gene            1294..1830
                     /locus_tag="PKN85_05545"
     CDS             1294..1830
                     /locus_tag="PKN85_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNT43903.1"
                     /translation="MKNHRPDWGSYFMEIARIVSMRSTCLRRNVGAVIVKDKRILATG
                     YNGAPSGLKHCLEVSCLRERLKIAPGERHELCRGLHAEQNAIIQAAYHGVSIDGAHLY
                     CTHLPCSICIKMLINAGIREVYYEDGYPDELSLSLIEESGIVLKRIDRVKATDPSARK
                     TPPASKGRTKRKTTSRNT"
     gene            1850..2254
                     /gene="nrdR"
                     /locus_tag="PKN85_05550"
     CDS             1850..2254
                     /gene="nrdR"
                     /locus_tag="PKN85_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNT43904.1"
                     /translation="MKCPFCNYVESKVLDSRMSKEMDTIRRRRECLKCGKRFTTAERL
                     EEGLPLVVKKDGRREVFDRTKILGGLNKACEKRPISVTNLEKIVSRIEYNLLEKGERE
                     IKATEIGEMVMSELKTRSPMSASLPFTGSSVI"
     gene            2316..3419
                     /gene="ribD"
                     /locus_tag="PKN85_05555"
     CDS             2316..3419
                     /gene="ribD"
                     /locus_tag="PKN85_05555"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNT43905.1"
                     /translation="MKDERYMRMALALAKKGMGTVSPNPMVGALLVKDDAIVGKGYHR
                     KAGSHHAEVGAIENAGENASGSTLYINLEPCVHYGRTPPCTDRIIAAGIKRVVVGMLD
                     PNPLVNGKGVEMLRKAGIDVKVGMLEDEARRLNEVFVGSMEKKRPFVTMKAAVSLDGK
                     IATKTCDSRWISNEESRRQANLLRCINDGVLVGINTVISDNPLLVPRLKNPKKIPVRI
                     ILDSKLRIPLACDVVKTSQKYRTWVFTNEDSRTDKETKLTALGVEVIRVPKDDNGRAS
                     VRHICEELYRREITSILVEGGGEVNSTLLREGLVDKVVLFYGTILIGGKGALNLVGGK
                     GIDFLKDAYRIDVATLKRLRENIYIEGYVHRNH"
     gene            3400..>3682
                     /locus_tag="PKN85_05560"
     CDS             3400..>3682
                     /locus_tag="PKN85_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNT43906.1"
                     /translation="MFTGIIEDIGVIAAIEKKSGSWVFVVKTSFEPGSVREGDSVSVD
                     GVCLTATAIRGQTFSVDASLETLRLTTLKDKTPGQKVNLERAMAADGRFG"
BASE COUNT          883 a         1058 c         1134 g          607 t
ORIGIN      
        1 caggatcagg aagatagacg atttcgagaa aaacacggac aggtaagaca tgaaacttga
       61 cggaaaagac acaaaggtat tcgagttgat gagacgcgaa ctggaccgcg aggaatacag
      121 tctcatcctc atcgcctccg agaactatgt agacgaggat gtcctcgcga cacagggatg
      181 cgtcctcacg aacaagtatg ccgaagggta tccgtcgaaa agatattaca gcggctgcgc
      241 ctatctcgat gagatagaaa gcattgccat agagcgcgcc aggacacttt tcggggtgga
      301 gcacgccaac gtccaacccc attccggctc gtcggcgaac atggccgttt tctatgccgt
      361 ccttgaacgc ggcgacacgg tcctggggat ggacatcgcc catggcggcc acctgaccca
      421 cggcgcgagg ccgagcttct cgggcaagtg gtaccgcgcg gtgtcgtacc cggtatcggc
      481 gaagaacgag atgctcgact acgacgagat acggggcatc gcccgcaagg aaagaccgaa
      541 gcttatcatc gcgggagcca gcgcctattc gaagatcata gacttcgcga aattcaggga
      601 gatcgccgat gaggtggggg cctacctgat ggccgatatc gcccacatcg ccggccttgt
      661 cgctgccggc cttcatccga gcccggcggg acacgcccat ttcataacta ccacgacgca
      721 caagaccttg aggggcccgc ggggcgggct cgtgatgtgc aacgccgacc tggcgaagaa
      781 gatagactcc gccgtcttcc ccggaatcca gggaggacct ctcatgcacg tcatcgccgc
      841 caaggcggtg gccctgagac aggccatgac ggaggagttc aaggagtacc agcgccagat
      901 catcgcgaac gcaaagcaca tggcgaagtg cctggccgac ctcggcttcc gcatcgtgtc
      961 cggaggcacc gacaaccatc tcttccttgt cgacctcacc ccgaagaacg tgacggggaa
     1021 cgatgcccag atagccctcg aggcgagcgg catcatggtc aacaagaacc tcatcccctt
     1081 cgacaccaag acccccggtg tcacgagcgg catcaggata gggacacccg ccgtcaccac
     1141 gcgcggtatg aaggaaaagg agatggaggt catctgcgag atgatcgatt ccgtcctgaa
     1201 gcggcccgga gacacgtcat tcatggacgg cacgcgagag agagtgaggg agatgtgcaa
     1261 gggctttccc ttctattcgc gcatatatga catatgaaga accatcgtcc tgactggggt
     1321 tcctacttca tggaaatagc acggatcgtt tccatgaggt cgacttgcct gcgccgaaac
     1381 gtgggagccg ttatcgtgaa ggacaagcgg atcctcgcca ccggctacaa cggggctcct
     1441 tcgggcttga agcactgcct ggaggtctcc tgcctgagag agagactcaa gatcgcaccg
     1501 ggggaacgtc atgaactgtg ccgcgggctc cacgccgaac agaacgcgat catccaggcg
     1561 gcatatcacg gggtatccat agacggcgcg catctctact gcacccatct gccgtgctcg
     1621 atatgtataa agatgctcat caacgcgggc atccgggagg tctattacga ggatgggtac
     1681 cccgacgagc tttccttgag cctcatcgaa gaatccggca ttgtgctgaa aaggatagac
     1741 agggtcaaag caacggaccc ttcggcccga aaaacacccc cggcgtcgaa gggcaggaca
     1801 aaacgaaaga caacgtcacg caacacctga gggtcgtcgg ggtggcctta tgaagtgtcc
     1861 cttctgcaac tacgtggaaa gcaaggtcct cgattcgagg atgagcaagg aaatggacac
     1921 gatccggcgc cgccgcgagt gcctcaagtg cggcaagcgg ttcaccaccg ccgaaaggct
     1981 cgaggaggga ctgcctctcg tcgtcaagaa ggacgggagg cgggaggtgt tcgacaggac
     2041 caagatcctc ggcggtttga acaaggcctg cgagaagagg cccatcagtg tcaccaacct
     2101 ggaaaagatc gtctcgagga tcgagtacaa tcttctcgag aagggcgaga gggagataaa
     2161 ggcgacggag atcggcgaga tggtcatgag cgagctcaag acgaggtcgc ctatgtccgc
     2221 ttcgcttccg tttacaggca gttccgtgat ataaatgagt tcatgagcga gctgacgcag
     2281 ctgctcctga agaaggacga ggagtgactc aacccgtgaa agacgaacgg tacatgcgca
     2341 tggcccttgc cctcgcgaaa aagggcatgg gaacggtgtc ccccaatccc atggtcgggg
     2401 ccctcctcgt gaaggacgat gccatcgtcg gcaagggcta tcacaggaag gccggctctc
     2461 accacgcgga ggtcggcgcg atcgagaacg ccggcgagaa cgcctccggc tccacactgt
     2521 acatcaacct cgaaccctgc gtccattatg ggaggacgcc gccctgcacg gaccggatca
     2581 tcgccgccgg catcaagagg gtggtggtgg gcatgctcga tcccaatccc ctcgtcaacg
     2641 gaaagggcgt tgagatgctg cgcaaggcag gcatcgatgt caaggtggga atgctcgagg
     2701 acgaggcccg ccgcctcaac gaggtgttcg taggctccat ggaaaaaaag aggcccttcg
     2761 tcacgatgaa ggcggcggtg agcctcgacg gcaagatcgc caccaagacc tgcgattcaa
     2821 ggtggatatc caacgaggaa tcgcgccggc aggcaaacct tctccgctgc atcaacgacg
     2881 gggtgctggt gggcatcaac acggtcattt ccgacaaccc ccttctcgta ccgaggctca
     2941 agaacccgaa gaagatcccc gtgaggatca tcctcgactc caagctccgt atcccgctcg
     3001 cctgcgacgt ggtgaaaaca tcgcagaagt acaggacctg ggtgttcacg aacgaggatt
     3061 cccgcaccga taaggagacg aaactgaccg cgctgggcgt cgaggtgatc cgggtgccaa
     3121 aggacgacaa cggcagggca tcggtgcgcc acatctgcga agagctttac cgccgggaga
     3181 taacgagcat tctcgtcgag gggggcggag aggtcaactc caccctcctg cgcgaagggc
     3241 tcgtcgacaa ggtggtcctt ttctacggca ccatcctcat aggcggcaag ggggcgctga
     3301 acctggtcgg cgggaagggc atcgacttcc tgaaggacgc ctaccggatc gacgtcgcca
     3361 ccctgaagag actgagggag aacatttaca tagagggata tgttcacagg aatcattgag
     3421 gacataggcg tgatcgccgc gatagagaaa aagagcggca gctgggtctt cgtcgttaag
     3481 acatccttcg agcccggcag cgtccgggaa ggcgacagcg tgagcgtcga cggcgtgtgc
     3541 ctgacggcga cggcgattcg cggccagacc ttcagtgtcg acgcctccct cgagaccctg
     3601 cgcctgacga ccctcaaaga caagaccccg gggcagaagg tgaacctcga gagggcgatg
     3661 gcggccgacg gccgcttcgg ag
//
LOCUS       DAOQTR010000187         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28500_length_3680_cov_4.157793, whole genome shotgun sequence.
ACCESSION   DAOQTR010000187 DAOQTR010000000
VERSION     DAOQTR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28500_length_3680_cov_4.157793"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="PKN85_05565"
     CDS             <1..1719
                     /locus_tag="PKN85_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43907.1"
                     /translation="RHHERVVKSRAKAVCELRLQKRGGSVFWARMESVPVTPGFDDGD
                     PVLLRTVFTDITERKEAEEALLEGEEKYRVLVESVNSIIARLDGEGRVIFLNDYGRDF
                     FGYSEEELWGTGLVGTILPPGQYGLDAKEFLIYMADHPEDYREREIEMVRRNGEKVWV
                     SWMVRTLYDEKGVFTGMLAVGNDVTERRRLEGEIRQAQKMEAIGTLAGGIAHDFNNIL
                     AGIIGFAEMIEEDSLPGSKDARRAQRIITAASRGADLVRQILAFSRKAEIAREPLSLS
                     PLIGETVRFLRSSLPATIEIQLAVKASDDTVLASPSEIQQILMNLVTNASFAMMDTGG
                     IIRAVITNVVFEPESPILDPDIEPGEYVQISVQDSGSGMSPDIMQRIFEPFFTTKGVG
                     QGTGMGLAVVFGIVKSLHGAISVESERGIGSTFRVLLPMARAAKKDEDPEAVLPPGGT
                     ERILFVDDEELIAEWGQTALEGLGYAVTVITDSEKALVVFTLDPSNFDLVIVDQTMPK
                     MTGFHLARRLLATRADIPIILCTGHSETVSPEYAKEAGIKEFLMKPLRKRQLAEAVRK
                     VLDTRE"
     gene            complement(1769..>3680)
                     /locus_tag="PKN85_05570"
     CDS             complement(1769..>3680)
                     /locus_tag="PKN85_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43908.1"
                     /translation="YRKLIGTRDIGFVVLNDKNVVVDANTKYARLAGRRDIDHVLGKS
                     ILDWTAQHDRDRLSRELAGCLKKGFARSVEIDHTDGEGGITPVEMNVTRIGAGSGKDL
                     VVFCRDVPKQTRTAGKLRYRAGGPAGLTEVAPFALRSTLFSVLENLPLGIILADRSGV
                     GTYVNPQFTGITGYTLDDVPTEKDWAVRAYPDLELRKTVVNAWKRNRSPGSIYESAER
                     PIACKNGREKVISLRATRLEKLTVITLSDAAKQGQTGSALRHNGDRYRSIYENAIEGI
                     FRAAPDGRFLSANPSHARMLGYDSSEDMVRDIASIGRELFLDPENHRQFRETLQTRGV
                     VEKFETRLRRRDGGTIWVSLNARAARDSAGEIAYYEGTAEDITDRKAMEEEKRQLEAR
                     LRQSEKMEAIGKLAGGIAHDFNNILEVLLGHCGILKMQAPKDGPVQERVRQIANTIEK
                     ASSFTRSLLAFSRKQDVVLKPVAINAMVRKSVRILEGLLPKTIALDMALHGGDATVLI
                     DVSKIDQVLLNLVANARDAMPRGGKITIRSRTVSLDEDFRRLYGYGTPGRYVLISVTD
                     TGQGIDDGVMHRIFEPFYTRKKPGKGTGLGLAIVYGIVKQHNGYINASSKPGKGTTFN
                     VYLPVATIRKPA"
BASE COUNT          699 a         1073 c         1118 g          790 t
ORIGIN      
        1 cggcaccacg aaagggtcgt gaagagcagg gcaaaggcag tttgcgaact gcggttgcaa
       61 aaacgcggcg gaagtgtctt ctgggcccgc atggagagcg ttcccgtcac gcctggtttt
      121 gatgatggcg atcccgtcct gctacgcacg gtattcacag acatcactga aaggaaagag
      181 gcggaggagg cgctccttga gggcgaggag aagtaccggg tacttgtcga aagcgtgaac
      241 agcatcatcg cgcggctcga cggcgagggc cgcgtcatct tcctgaatga ttacgggcgg
      301 gacttcttcg gttactccga ggaggaattg tggggaacgg gactcgtggg gacgatattg
      361 cctcccgggc agtacggttt ggacgcaaag gaatttctca tctacatggc ggaccatccc
      421 gaggattatc gggaaagaga gatcgagatg gtccgacgca atggggaaaa ggtctgggtc
      481 tcgtggatgg tccggacact gtacgatgaa aagggtgttt tcaccgggat gcttgcggtg
      541 ggaaacgatg tcaccgaacg caggaggctg gaaggtgaga tccggcaggc gcagaagatg
      601 gaggccatcg gcactctcgc cgggggcatc gcccatgatt tcaacaacat ccttgcgggc
      661 atcataggat tcgcggagat gatcgaggag gattccctcc ccggcagcaa ggatgcccgg
      721 cgggcacaaa ggataataac cgccgcctcg cgcggggccg atcttgtccg ccagatactg
      781 gccttttccc gcaaggccga gattgccagg gagcccctgt cgctttcacc cctgatcggg
      841 gaaacggtgc gtttcttgcg gtcgtctctc cctgccacga tcgagatcca actcgccgtc
      901 aaggcgtccg atgacaccgt actggcgtct ccttcagaga tacagcagat cctgatgaat
      961 cttgttacca atgcctcttt tgccatgatg gacacgggcg ggatcatcag ggcagtgatc
     1021 acgaatgtcg tcttcgagcc cgaatcccct atcctcgatc cggacattga acccggcgaa
     1081 tatgtgcaga tctccgtgca ggattcaggc tccggtatga gccccgacat aatgcaacga
     1141 atcttcgaac ctttctttac cacaaaaggt gtcggccagg ggacgggcat gggcctggcg
     1201 gttgtcttcg gcatcgtgaa gagcctccat ggcgccataa gcgttgagag cgaacggggg
     1261 atcggttcca cgttccgggt tctcctcccc atggcaaggg ccgctaaaaa agatgaagat
     1321 cctgaagcgg tcctgccccc cggggggacc gaacgcattc tgttcgtcga cgacgaggaa
     1381 ctgatagcgg aatggggtca gaccgcgctg gaaggtttgg ggtacgccgt aacggttatc
     1441 acggacagcg agaaggcgct cgtcgtgttc actctggacc cgtccaactt cgacctcgtc
     1501 atcgttgatc agacgatgcc gaagatgacc ggtttccacc ttgccaggag gttgctcgcg
     1561 acacgcgccg atatccccat catcctctgc acgggccaca gcgagacggt ttcaccggaa
     1621 tacgcaaagg aggcggggat aaaagagttc ctgatgaaac ccctcaggaa aaggcagctc
     1681 gccgaagcgg tgaggaaggt cctcgatacc agggaatgat ccacggggac tcctccggca
     1741 ccgccccgaa catgaaccgc tcttccggtc atgccggctt tcggatcgtt gccacgggca
     1801 aatagacgtt aaaagtcgtt ccttttccag gtttgctgga ggcgttgatg tacccgttgt
     1861 gctgtttcac tattccgtag actatggcaa ggccgaggcc cgttcccttt cccggtttct
     1921 tcctggtata gaagggttcg aatatcctgt gcatcactcc gtcatcaatg ccttgtcccg
     1981 tatcggtaac ggagatgagg acgtacctgc cgggggtgcc gtagccgtaa agcctccgga
     2041 aatcttcgtc gaggctgacc gttctcgacc gtatggtgat cttgccgccc cggggcatgg
     2101 cgtcgcgggc attggcgacg aggttcagca atacctggtc gatctttgat acatcaatga
     2161 ggactgtcgc gtccccgccg tggagggcca tatcgagggc gatggtcttc ggcagcagcc
     2221 cctcaaggat gcgcacgctc tttcgaacca tcgcgttgat cgccacgggt ttgagcacga
     2281 cgtcctgttt cctgctgaac gcgagaaggc ttctcgtgaa gctcgatgcc ttctctatcg
     2341 tgttggcgat ctgcctcacg cgttcctgca ctggaccgtc cttcggcgcc tgcatcttca
     2401 gaatgccgca gtggccaagg aggacctcga ggatgttgtt gaagtcgtgg gcgatgcccc
     2461 ctgccagctt gccgatggcc tccatctttt ccgattggcg aagccgcgct tcgagctgtc
     2521 tcttctcctc ctccatcgcc ttccggtccg tgatgtcttc ggcggtccct tcgtagtagg
     2581 cgatctcgcc cgcgctgtcc cgcgccgcgc gggcgttgag ggagacccat atggtgccgc
     2641 cgtccctgcg acgcagccgg gtctcgaact tttcgacgac gccccgcgtt tgcagggtct
     2701 cacggaattg ccggtggttc tcggggtcca ggaagagctc tcgacctatg ctggcaatgt
     2761 cccggaccat gtcctccgac gaatcgtatc cgagcattct tgcatgcgac gggttcgcgc
     2821 ttaagaaacg cccatccggc gcagcccgga agatgccctc gatggcgttc tcgtatatgc
     2881 tgcggtacct gtctccattg tgcctgaggg ccgaccctgt ctgtccctgt tttgctgcgt
     2941 cgctcaaggt tatgacggtg agtttctcga gacgggtcgc ccgcaacgat atgacctttt
     3001 cccgtccatt cttgcaggcg atcgggcgct ccgctgactc atagatgctt cccggcgaac
     3061 gatttctctt ccaggcgttg accaccgttt tcctgagttc caggtcgggg tacgcccgga
     3121 cagcccaatc cttctcggtg ggcacgtcat cgagggtgta gccggtgatg ccggtgaact
     3181 gcgggttgac gtacgtgccc accccggaac gatcggccag gatgatgccg agaggaaggt
     3241 tctcaaggac gctgaagaga gtgctcctca acgcgaaggg ggcaacctcc gtgaggcccg
     3301 ccggcccgcc ggcccggtat cggagctttc cggccgtacg tgtctgcttg gggacatcgc
     3361 gacagaacac gacaagatcc ttcccggagc cggcaccgat gcgcgtgacg ttcatctcga
     3421 cgggcgtgat gccgccctcc ccgtcagtgt ggtcgatctc gacgcttctg gcgaaacctt
     3481 tcttcaggca tccggcgagc tcccgggaaa gcctgtcccg gtcgtgctgc gcggtccagt
     3541 caaggatgct ctttccaagc acatgatcga tgtcgcggcg gcctgcaagg cgggcgtatt
     3601 tcgtattcgc gtccacaacg acgtttttgt cgttgaggac cacaaagcct atgtcccgtg
     3661 ttccgatcag cttccgatac
//
LOCUS       DAOQTR010000188         3670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28620_length_3670_cov_3.449793, whole genome shotgun sequence.
ACCESSION   DAOQTR010000188 DAOQTR010000000
VERSION     DAOQTR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28620_length_3670_cov_3.449793"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="PKN85_05575"
     CDS             complement(<1..1703)
                     /locus_tag="PKN85_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43909.1"
                     /translation="MSITSGAAGNDLQRKEKRRVANPAGTAILAVFSFLLFAGACGAF
                     TTERHKTALFLHSYHRTDWTETIMEGYISALQGRDDLGVHVEYMDTKKTETAEYLRKL
                     RDIYAMKFGAIRFDVILVSDDNAFRFVLSHHKDLFSYAPIVFCGVNRSSRDPVAGRNN
                     VVGVLEGDDFEETLAFAFRVRPKTRTVYVVHDRTKENLKRLEELSRIIRAGYPRVTVE
                     TLGDLSYSRILEAMPTLPRDSIVFFISLWQDAEGRPVSAREASRILRLSPVPVFGRSG
                     SLVGKGLVGGKCVSGFSQGRAAGLLARQILDGKVPGDLPRVIESPNRFMFDHAELVEH
                     GIAENVLPPGSVILNKPPSFFEQNRSTILTAIAVIIVLLAFIAVLVIGLARLKRTSLK
                     LRSREQELLASQETLSGILSASPSGIVKIRGRVFEWVNDAMCSITGYEEDELVGHDSR
                     FLYPDDEEYERVGREIYRKGQEESRWVRKDGTVIELLFQVAKATGDSYIAIITDITER
                     IRTARALRESEKLYRNIIEKIQDVFYRFDINGILIMINPAGARMFRYDSVEDMLGLPM
                     EA"
     gene            2241..3452
                     /locus_tag="PKN85_05580"
     CDS             2241..3452
                     /locus_tag="PKN85_05580"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT43910.1"
                     /translation="MVHYAWIIAFTGTLVTILAHGFGRMSYSVILPPMKDGLVLNYTQ
                     LGSIATGNFIGYLSLAIIGGFLAARFGVRRVVFVSLLVIGVSLFLTGLADSFSFAFVM
                     RLIAGLGNGGSYVPIMALPAAWFIMKKRGLATGIVSAGIGTGLFLAGIILPPIISHFG
                     KEGWRYAWYFLGIAVFVFAFVCYAFLRNNPAEKGLSMYGGTEEQKGGPRVTLFSAFKD
                     VVVESEIWKLGMVYFMYGFSYIIYLTFFVAFLTKEMGVSPIAAGRIFAVLGIFSIFCG
                     VIYGWISDVLGRRYGSMIAYLTLALSYVIFAFWKDTTGFYVSAVVFGIAAFSIPTIMA
                     AASGDAVGGRLAPAGLGFITLFFGIGQALGPLAGGYIKDATGTFTNAFLLSAGVSLLG
                     AFGSLILKKKV"
BASE COUNT          728 a         1092 c         1012 g          838 t
ORIGIN      
        1 gcctccatgg gcaggccaag catatcctcg acggaatcat agcgaaacat cctggccccg
       61 gcggggttta tcatgatcag gataccgttg atgtcgaacc tgtagaagac gtcctggatc
      121 ttctcgatta tgtttcggta gagcttctcg ctctccctca gggcccgggc cgtcctgatg
      181 cgttccgtta tgtccgttat gatggcgatg tagctgtcgc ccgtcgcctt cgccacctgg
      241 aagaggagtt ctatgacggt accgtccttg cggacccacc tggattcctc ctgccctttg
      301 cggtagatct cgcggcccac cctctcgtac tcttcgtcat cgggatagag gaatcgtgaa
      361 tcgtgtccca caagctcatc ttcctcgtaa cccgttatgg aacacatcgc atcgttcacc
      421 cattcaaaga ccctgcccct gatcttcacg ataccgctcg gcgaggcgga gaggatcccc
      481 gacagcgtct cctgtgaggc aaggagttcc tgctctctcg acctcaactt caaggacgtg
      541 cgcttcaggc gcgcgagacc gatcaccagg accgcgatga acgcgagcag aacgatgatc
      601 accgcgatgg cggtgagtat ggtgctcctg ttctgttcaa aaaaggaggg cggcttgttc
      661 aggatgacgc tgcccggagg gagcacgttc tcggcgatcc cgtgttccac cagttccgcg
      721 tggtcgaaca tgaaccggtt cgggctctcg atcacccggg gaaggtcccc ggggactttg
      781 ccgtccagta tctggcgcgc gaggagtccc gcggccctgc cctggctgaa accggagacg
      841 catttccctc caacgagccc tttgcccact aaagagcccg agcggccgaa gaccgggacc
      901 ggtgacagtc tcaggatcct cgaagcctcc cgcgccgaaa cgggcctgcc ctcggcgtcc
      961 tgccacaggg atatgaagaa cacgatgctg tcccggggaa gcgtgggcat ggcctcaagg
     1021 atccgcgaat aggagaggtc gcccagcgtc tccaccgtca cccggggata gccggccctg
     1081 attatccgcg acagttcttc aaggcgcttg aggttctctt ttgtccggtc gtgcacaacg
     1141 tagaccgttc gcgtcttcgg cctcacccgg aaggcaaacg ccagcgtttc ctcgaaatca
     1201 tccccttcaa gcacccccac gacattgttc cggccggcga cgggatcccg gctggacctg
     1261 ttgaccccgc agaacacaat gggagcatag ctgaagagat ccttatgatg ggataagaca
     1321 aatcgaaagg cgttgtcatc ggagacaagg ataacatcga accgtatggc gccaaacttc
     1381 atcgcgtaga tgtcgcgaag cttgcggaga tactcggcgg tctccgtctt cttcgtgtcc
     1441 atgtactcca cgtgcacgcc aaggtcatcg cgcccctgca aggcggatat atagccttcc
     1501 attatggtct cggtccaatc tgtcctgtgg taggagtgga gaaagagagc cgtcttgtgg
     1561 cgctccgtcg tgaatgcccc gcaagcgccg gcgaagagaa gaaacgagaa aaccgcgagt
     1621 attgccgtac ccgccgggtt ggctacccgc ctcttctcct tccgctggag gtcattacct
     1681 gccgcgcctg atgtgatgct catgcgttga gatactcgcg taatcctgtt cctcgatgaa
     1741 caatttacga tcacggccgc ggatcgtttc tcatgccctg caacgcagca ctctgaagcg
     1801 atgctgtgaa caaaccttat gatacttatc ggcacttccg caagagaaat taacagagcc
     1861 accccgaatg acggtttgcc tgccatcagt gttagcgggt taacagaagc caggatcgaa
     1921 gccggttata tttggagagg aaccggagca ctcggagaac agcagaagtt acggtgcccg
     1981 tgaggatggg tgccggtggt cataccggtc ttccctcgag cggtccgcat tggtctcacc
     2041 cgtcaatcgg gcttgcattt tgcgggatga caacgtacca tagttcttaa gcgggggtta
     2101 caacggcggg tgaacggccg ggagaagcaa ctcgaaataa cgctctcatc gtccatggca
     2161 ggattgtgat ctgaaacaga aggcatacct gcaccgtcgc tttattgaag cgcattgcat
     2221 tccaatccgg aggtggaata atggtgcatt acgcatggat catcgctttc accggtacac
     2281 ttgtcaccat tctggcccat ggcttcggca ggatgtccta ttcagtgata ctgcctccca
     2341 tgaaagatgg cctcgtgctc aactatactc aactcggttc catagcgacg ggcaatttta
     2401 tcggctacct cagccttgcc atcataggcg gctttctcgc cgccaggttc ggagtgagac
     2461 gggtcgtttt cgtctctctc ctcgtgatcg gtgtaagcct ctttctcacg ggtctagccg
     2521 actccttctc cttcgccttc gtcatgcgac tgatagcagg gctcggtaac ggcgggagtt
     2581 acgttcccat catggccctg cccgcggcct ggttcatcat gaagaaaagg ggcctggcca
     2641 ccgggatcgt atccgccggc atcggcaccg ggcttttcct ggcgggcatc atcctgcctc
     2701 ccatcatctc gcacttcggc aaggaggggt ggcgctacgc ctggtatttc ctcggaatcg
     2761 cggtgttcgt tttcgcgttc gtctgctacg ccttcttgag gaacaatccg gctgagaagg
     2821 gtctttccat gtacggtggt actgaagagc agaaaggcgg cccaagggtc accctcttct
     2881 cggccttcaa ggacgtcgtc gtcgaatcgg agatctggaa gctgggaatg gtctatttca
     2941 tgtacggctt ctcctacatc atatatctca cttttttcgt cgccttcctc accaaggaga
     3001 tgggcgtgag ccccatagcc gcgggcagga tcttcgccgt cctgggcatc ttcagcattt
     3061 tctgcggcgt catatacggc tggatatccg atgtgctcgg aagaaggtac gggtccatga
     3121 tcgcctacct gaccctggcc ctttcctacg tgatcttcgc cttctggaag gacaccaccg
     3181 gtttctacgt atcggcggtc gttttcggga tcgccgcctt ctcgatcccc accatcatgg
     3241 ccgcggcgtc gggagacgcc gtgggcggac ggctcgcccc tgccggcctc gggttcataa
     3301 cactcttctt cgggataggc caggccctcg gccccctggc aggaggctac ataaaggacg
     3361 ccaccggaac cttcaccaac gcgttcctcc tttcggcagg tgtctcgctg ctcggtgcct
     3421 tcggttctct catcctcaag aagaaggttt gacagcttgg agcggtcccg ggtcgcacgt
     3481 ctcaggcaaa tgacagttgc gggttccggg tcaaagacca aagaatacaa gaatgctgta
     3541 aaggttccgg tcttcccgga cagcctgaag gtctccccgc atttctttct ccgtcattcc
     3601 ggctttagac cggtgtcgtt gatcaaaaat gttttgacaa aaatcgtaac agattttaaa
     3661 ctgtaccgtc
//
LOCUS       DAOQTR010000189         3669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28629_length_3669_cov_3.451577, whole genome shotgun sequence.
ACCESSION   DAOQTR010000189 DAOQTR010000000
VERSION     DAOQTR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28629_length_3669_cov_3.451577"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3192
                     /gene="dnaE"
                     /locus_tag="PKN85_05585"
     CDS             <1..3192
                     /gene="dnaE"
                     /locus_tag="PKN85_05585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT43911.1"
                     /translation="VVLLAMDNRGYQNLLKLISLAHLEGFYYHPRIDREILSQYHEGL
                     ICLTACIKGEIPNAILRGTDEMVRNHVDYYLSLFGDRLFFELQDNGLEEQRIVNERLI
                     ELSSHYGVPIVATNDCHYLRREEAKAHELLLCIQTGKTINDKDRLSFSTDQFYFKSPD
                     EIALAFSRYPEALANTMRVAEMCNVTIDTNTYHFPEFHPPAGMDLDEYFEKLARQGFE
                     KRMPEIRAAYDSFGEELLDQYRKRFDYEAGVIRKTGFSGYFLIVADFINYAKTHGVPV
                     GPGRGSAAGSLIAFCLGITDIDPIKYDLIFERFLNPERISMPDIDVDFCRKGRDRVIE
                     YVTNKYGKDNVAQITTFGTMKSKAAVRDVGRALGMPYAEVDKIAKLITTADRGIERAI
                     NDEPEVRELYQRDDRVRELLDNACVVEGLARHASTHAAGIVIANKHLSEYLPLYRGKH
                     DETVTQYDMKMIEKIGLIKIDFLGLETLTLMDTIVKLLKAEGIEIDLARLPLDDTKTY
                     ELLSSGNTSGVFQLESRGMRDLLTKLRPSQFDDIMPLIALYRPGPLKSGMVDEFIKRR
                     NNPSLVAYETPKLKDILHDTYGVIIYQEQIMKIASVLAGFSMKDADALRKAMSKKIPE
                     QLEKYKQQFVDGAVANDVPKDVAEKIYDVIQRFGEYGFNKSHSTAYGYIAYQTAYLKA
                     HYPTHYFAAMLTTEVGDTDKLVKYVGECRESGIEILPPDINMSQSDFTIVDSKIRYGL
                     SGVRNVGEAAIESIIQAREEAGGFKSFVHFCSVIDSRKVNKKVIESLAMAGCFDSLGL
                     NRSQVLYLAQEMLDKLTKRDTKNNHKQGSIFGEAEVAETTVSFDIPAMDELSHEEILL
                     GEKESLGFYFSKHPLKPYEKLIAELTPYDSQGLKEADTEQDVSMAGIVSALKEITTRR
                     GDRMAYVTLEDTKGIVEVICFPDLYSKNHFVIQSGKPLMVTGSLERSEDGSARIKAKA
                     VALLEALTGELLKTVRIRIHCEVIRKEDLRVLKDILFSLRGRSGVLLEFQLNGERRSL
                     PLKNVRIDPGRKDVILKHFKKGMDVEVIDEILP"
     gene            3176..>3669
                     /locus_tag="PKN85_05590"
     CDS             3176..>3669
                     /locus_tag="PKN85_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNT43912.1"
                     /translation="MRYYLDFEKKLEPLERRVFEIERFYDENEPHYAKELATLRKKIA
                     KIEKETYSELSNWQRSQLSRHLNRPHTLDYVHSLFTDFVELHGDRKFKDDPALVAGFA
                     RFEGVNVAVLGHQKGGDVREMAHRNFGMAHPDGYRKAMRVMDMAARWGKPIITFIDTP
                     GAFPG"
BASE COUNT          880 a         1154 c         1015 g          620 t
ORIGIN      
        1 gtcgtcctcc tcgcgatgga caacaggggg tatcagaacc tcctcaagct catcagcctc
       61 gcccacctgg aaggtttcta ctaccacccc cgcatcgaca gggagatact gagccagtac
      121 cacgaaggtc tcatctgcct cacggcgtgc atcaagggag agataccgaa cgccatcctc
      181 cggggcaccg acgagatggt ccgcaaccac gtggattact acctctcact cttcggcgac
      241 cggctcttct tcgagctcca ggacaacggt ctcgaggagc aaaggatagt caacgaacgc
      301 ctcatcgagc tctcgtccca ttacggggtg cccatcgtcg cgacgaacga ctgccactac
      361 ctgcgccgcg aggaggcaaa ggcccacgag cttctgctgt gcatccagac gggaaagacg
      421 atcaacgaca aggaccgcct gagcttctcg acggaccagt tctacttcaa gtcgcccgac
      481 gagatagctc tcgccttctc ccgctatccc gaggcgcttg ccaacacgat gcgcgtcgcc
      541 gaaatgtgca acgtcaccat cgacacgaac acgtaccact tcccggaatt ccaccccccc
      601 gccggcatgg acctcgacga gtacttcgaa aagctcgcgc ggcagggttt cgagaaacgg
      661 atgcccgaga tacgggcggc ctacgattcc ttcggcgaag agctcctcga ccagtatcgc
      721 aagaggttcg actacgaggc gggcgtcatc aggaaaacgg ggttctccgg gtatttcctc
      781 atcgtcgccg acttcatcaa ctacgcgaag acccacggcg ttcccgtggg gccggggcgc
      841 ggttccgcgg cgggcagtct catcgccttc tgcctcggca tcaccgacat cgaccccatc
      901 aagtatgacc tcatcttcga gcgcttcctg aacccggagc gcatcagcat gcccgacatc
      961 gacgtcgact tctgccgcaa gggacgcgac agggtcatcg aatacgtcac gaacaagtac
     1021 ggcaaggaca acgtggccca gatcaccacc ttcgggacca tgaagtccaa ggcggcggtg
     1081 cgtgatgtgg ggcgggccct cggcatgccc tacgcggagg tcgacaagat agcaaagctc
     1141 atcaccaccg cggaccgggg catcgaacgg gcgatcaatg atgaacccga ggtgcgggaa
     1201 ctataccaga gggacgaccg cgtgcgggaa ctcctcgaca acgcctgcgt cgtcgaaggg
     1261 ctggctcgcc acgcttccac gcacgccgcg ggcatcgtga tcgccaacaa gcatctctcc
     1321 gagtaccttc cgctgtacag gggcaaacac gatgaaacgg tgacgcaata cgacatgaag
     1381 atgatcgaga agatcggtct catcaagata gacttcctcg gcctcgagac cctcaccctc
     1441 atggacacga tcgtgaagct cctcaaggcc gaaggcatcg agatcgacct cgcccggttg
     1501 ccccttgacg acacaaagac ctacgagctc ctttcgtcgg gcaatacctc cggagtcttc
     1561 cagcttgaaa gccgcgggat gcgcgacctt ctcacgaaac tgaggccctc acagttcgac
     1621 gacatcatgc cgctcatcgc cctgtacagg cccggtccct tgaaaagcgg catggtcgac
     1681 gagttcatca agcgccgcaa caacccgtcg ctcgtggcct acgagacgcc gaagctcaaa
     1741 gacatcctcc acgacacgta cggcgtcatc atctaccagg aacagatcat gaagatcgcc
     1801 tccgtgctcg ccggcttctc catgaaggat gccgacgcgc tccgcaaggc catgagcaag
     1861 aagatccccg aacaactcga gaaatacaag cagcagttcg tcgacggggc cgtggcgaac
     1921 gacgtcccga aggacgtggc ggagaagatc tacgacgtca tccagcgttt cggggaatat
     1981 ggcttcaaca agtcccacag cacggcctac ggctacatag cctaccagac ggcatacctc
     2041 aaggcccatt accccaccca ctatttcgcg gccatgctca ccaccgaggt gggtgacacg
     2101 gacaagctcg tcaagtacgt cggggaatgc cgggagtcgg gcatagagat cctgccaccc
     2161 gatatcaaca tgagccagag cgatttcacc atcgtcgatt cgaagatccg ctacgggctc
     2221 tccggcgtca ggaacgtggg cgaagcggcg atcgaaagca tcatccaggc ccgcgaggaa
     2281 gcgggagggt tcaagtcctt cgtccacttc tgcagcgtca tcgattcccg caaggtcaac
     2341 aagaaggtca tcgaaagcct tgccatggca ggctgcttcg acagtctcgg cctcaacagg
     2401 tctcaggtcc tctatcttgc ccaggaaatg ctcgacaaac tgacgaagcg ggacacgaag
     2461 aacaatcaca agcagggcag catctttgga gaggctgagg tcgccgagac gacggtctcc
     2521 ttcgacatcc ccgccatgga tgaactctcc cacgaagaga tcctccttgg cgagaaggaa
     2581 tcccttggtt tctacttcag caagcatccc ctgaaaccct atgaaaaact catcgccgaa
     2641 ctcacccctt acgacagcca gggcctcaag gaggctgaca ccgaacagga cgtgagcatg
     2701 gcgggcatag tgagcgccct gaaagagata acgacaaggc ggggcgacag gatggcgtac
     2761 gtcaccctgg aggacacgaa gggcatcgtg gaggtcatct gtttccccga tctttacagc
     2821 aagaaccact ttgtcatcca gagcggaaaa cccctcatgg tcacggggtc gctcgagagg
     2881 tccgaggacg gcagtgcccg gataaaggcg aaggccgtgg cccttctcga agcactgacg
     2941 ggtgaactcc tgaaaaccgt caggatacgg atacactgcg aggtcatcag gaaagaggac
     3001 ctgagggtgt tgaaggatat cctcttcagc ctccggggca ggtcgggcgt tcttctcgag
     3061 ttccagctca acggggaaag acggtccctc cccctcaaaa acgtcaggat cgaccccgga
     3121 cggaaagatg tcatcctcaa gcacttcaag aaaggcatgg acgtagaggt catagatgag
     3181 atactacctt gatttcgaaa agaagcttga accgctggaa agacgggtct ttgagataga
     3241 acggttctac gacgagaacg agccgcacta cgcgaaggag ctcgcgaccc tcaggaagaa
     3301 gatcgcaaag atagagaagg agacatactc cgaactgtcg aactggcagc gctcccagct
     3361 ctcgcgacac ctcaaccgcc cgcacaccct tgattatgtc cacagtctct tcaccgactt
     3421 cgtggagctg cacggcgaca ggaagttcaa ggacgacccg gcgctcgttg ccggttttgc
     3481 ccgcttcgaa ggagtcaacg tggccgtcct cggccatcag aagggcggtg acgtccgcga
     3541 gatggcccac aggaacttcg gcatggccca tcccgacggc taccgcaagg cgatgcgcgt
     3601 catggacatg gccgcgagat ggggcaaacc catcataacc ttcatcgaca cgcccggggc
     3661 cttccccgg
//
LOCUS       DAOQTR010000190         3669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28631_length_3669_cov_2.835086, whole genome shotgun sequence.
ACCESSION   DAOQTR010000190 DAOQTR010000000
VERSION     DAOQTR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28631_length_3669_cov_2.835086"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PKN85_05595"
     CDS             <1..503
                     /locus_tag="PKN85_05595"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HNT43913.1"
                     /translation="SIRIYPAPAETATGTCVVICPGGGYGALAFDHEGHQVARWFNTF
                     GVTAFTSYGLFWWTLVFILVFKGLPGNPEAFMGWYLFMWGLFTFFMWFGTWGKNRAIQ
                     FVFLSLTILFWLLAIRDWSGSAAIGTLAGWEGIICGLSAVYLAMAEVLNEVHGRTVLP
                     IGEIKK"
     gene            complement(649..1461)
                     /locus_tag="PKN85_05600"
     CDS             complement(649..1461)
                     /locus_tag="PKN85_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43914.1"
                     /translation="MMGGIEESCYAILGLPVGAPQEELEEAFRRLKRESEGDWDRLKE
                     ITWAYETLAGSRAGAGKEGNGGTGLTGPGDENETTLIGLLFPPQVKVNPFYFWGRCLV
                     FMLLLIAGVRLMVNPPSSELAGHSFFHNISLPFHEAGHIVFRILGDFMGVLGGSLMQV
                     LIPVICLAAFLKRGDAFAASFALWWTGQNFIDMAPYINDARAQQLMLLGGVTGQDVPG
                     YHDWNNILGRLGLLNLDHGLANASHIVGSLLMTAAFIWAAIILWMQWKNLDR"
     gene            complement(1599..1733)
                     /locus_tag="PKN85_05605"
     CDS             complement(1599..1733)
                     /locus_tag="PKN85_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43915.1"
                     /translation="MMKQARTILSSLGKKISVLRRGSKIVLVLCAWLLVISMLLAFGL
                     "
     gene            1927..2448
                     /locus_tag="PKN85_05610"
     CDS             1927..2448
                     /locus_tag="PKN85_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43916.1"
                     /translation="MKSACLAIAVLLVLCFFFAGCGEKEVPRTVKVYPLDNMEGVLTQ
                     SNVAFDRAVSADGRGSLRIDAPSPMTVRLYEVKDIDVDRARLTYQAKLKTQDFKGKVY
                     LEMWCVFTGRGEYFSRDLENSVTGTTDWVTQETPFFLKKGQKPDIVRLNLVVDGNGTV
                     WIDDVKLVRGPLK"
     gene            2528..2986
                     /locus_tag="PKN85_05615"
     CDS             2528..2986
                     /locus_tag="PKN85_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HNT43917.1"
                     /translation="MKYQVGETGRVVLAHFEDGDEIISNLSGIIRKENIRGAIFYLLG
                     GIAEGRIVVGPMQDDARPPEPTWRELVESHETLAVGTVFWQGDEPRFHIHGTYGKFDA
                     VKTGCLRGEARTFLIMEAVIVELKGINAVRELDPVINMTLLKLTGGGSIS"
     gene            3143..3550
                     /locus_tag="PKN85_05620"
     CDS             3143..3550
                     /locus_tag="PKN85_05620"
                     /inference="COORDINATES: protein motif:HMM:NF037472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HNT43918.1"
                     /translation="MKGGKIMKYGMACCLLMLTVLIGPEVAVAGVMDKDFEVGTTREL
                     VNLCSVAAGDPMAKEAIHFCQGYIVGAFDYHVAQSAGPGGKCLVCMPDPPPDRDKVVA
                     MFVAWAREHPQYMGDKPVETQFRFLIEKWPCAR"
BASE COUNT          782 a         1010 c         1067 g          810 t
ORIGIN      
        1 cgtcgatccg catctacccc gcgccggccg agaccgcgac cggaacctgc gtcgtcatct
       61 gtcccggcgg cggctacggg gcgctcgcct tcgaccatga agggcatcag gtggcccgtt
      121 ggttcaacac cttcggcgtg acggcgttca cctcgtacgg tctcttctgg tggacccttg
      181 tgttcattct cgttttcaag ggccttcccg gaaatcccga ggctttcatg ggatggtatc
      241 ttttcatgtg gggcctcttc accttcttca tgtggttcgg cacatggggc aagaacaggg
      301 ccatccagtt cgtgttcctg agcctcacca ttctcttctg gcttcttgcc atccgtgact
      361 ggagcggcag cgccgccatc ggcacccttg ccggatggga aggcatcatc tgcggcctct
      421 ccgccgtcta cctggccatg gccgaggtcc tcaatgaagt ccacggaagg actgtacttc
      481 ccatcgggga gataaagaaa taaagacgga ttgaatcgct tgaggggttt gaatcgtttg
      541 agagttagaa gacaaagagg ccaactctcg atcgttcgaa cccctcaagc cgtttcttga
      601 tctttcaccc tcaagctttt caagcgattc aagctactca agccgctttt atctgtccaa
      661 attcttccac tgcatccaga gtatgatggc tgcccagatg aaggcggctg tcatcaggag
      721 ggagccgacg atgtgggatg cgttggccag gccgtggtcg aggttgagga ggccgaggcg
      781 gccgaggatg ttgttccagt cgtggtatcc ggggacatcc tggcccgtca cgcctcccag
      841 gagcatcagt tgctgagctc gggcgtcgtt tatgtagggg gccatatcga tgaaattctg
      901 tcccgtccac cacagggcga aggacgccgc aaaggcgtcg ccccgtttca ggaaggcggc
      961 gagacagatg acgggtatga gcacctgcat gaggcttcct cccagcacgc ccatgaagtc
     1021 gccgaggatg cggaagacaa tgtgtccggc ctcgtggaag gggagactga tattgtggaa
     1081 gaacgaatgg cccgccagct cgcttgacgg cggattgacc atgagcctca cgccggctat
     1141 gagaagaagc atgaatacca ggcatcttcc ccaaaaatag aaggggttta ccttcacctg
     1201 gggagggaag agaagaccga tgagagtggt ttcgttttca tcacccggtc cggtgaggcc
     1261 cgttcccccg tttccttctt tccccgcgcc ggcccgggat cccgccagcg tctcgtaggc
     1321 ccaggtgatc tccttgagcc tgtcccagtc gccctccgat tccctcttca ggcgtctgaa
     1381 agcctcctcc agctcttcct gtggtgcccc cacgggaagg ccgagaatgg cataacagct
     1441 ctcctcgata ccacccatca ttctaaccta ccatgtcaac cgacagggag caaacagggt
     1501 ttcaagtttc aggtttcaag ttacatgctt cagctcaagg aaggggacac cttctcgttc
     1561 tggcctgaaa catgaaacct tggaacctgg gaccgcctct acagtccgaa cgccagcagc
     1621 atgctgatca cgagcagcca cgcgcacagc acaagtacga tcttggagcc cctcctgagg
     1681 acagagatct tcttcccaag ggaggagagt atcgtacgag cttgtttcat catgtcttca
     1741 cctcggtgct gatgctattt caggttatcg gaaagttccc cgtattatta aataccagcc
     1801 gctcctgcgc ctctttcccg gatctttgcc gcgaacatct cttcaccctg gcggcctgtt
     1861 gtgataggat aatgctatag gacgacgtca ggagtccttt gaaatgctgc ggaaggagac
     1921 catcccatga aaagcgcatg tcttgcgata gcagtccttc tggttctctg cttctttttt
     1981 gcgggctgcg gcgagaagga agtgccccgg actgtcaagg tctatcccct cgacaacatg
     2041 gagggggtcc tgacgcagtc gaatgtcgct ttcgatcgcg ctgtgtcggc ggacggcagg
     2101 ggttccctga ggatcgatgc cccgtcgccc atgacggtcc ggctctatga ggtcaaggac
     2161 atcgacgtgg acagggcacg tctgacatac caggcaaaac tgaagaccca ggacttcaag
     2221 ggcaaggtct acctggagat gtggtgcgtc tttaccggaa ggggtgaata tttctcccgt
     2281 gacctggaaa actccgtgac aggaacaacc gactgggtca cacaggaaac acctttcttt
     2341 ctcaagaagg ggcagaagcc cgatatcgtc aggctgaacc ttgttgtcga tggaaatggc
     2401 acggtctgga tcgatgatgt gaagctcgta agaggacctt tgaaatgacc cttcagtgag
     2461 acttgtcggc atcctgctcg gaggttgacg tctttcctcc cggcaagccg atgaaaagga
     2521 gatcgcagtg aaatatcagg tcggtgagac gggcagggtg gtgctggccc attttgagga
     2581 cggcgatgag ataatctcca atctttccgg tatcatcagg aaggagaaca tcaggggtgc
     2641 catcttctat ctcctcggag ggatagcgga gggcaggatc gtcgtcgggc ccatgcagga
     2701 cgacgcgcga ccgccggaac cgacatggcg ggaactcgtc gaaagccacg agaccctggc
     2761 cgtcggcacc gtcttctggc agggtgacga gccgcgcttt catatccacg ggacctacgg
     2821 gaagttcgac gccgtgaaga cgggatgcct tcggggcgag gcgaggacct ttctcataat
     2881 ggaggcggtc atcgtcgagc tcaagggcat caacgccgtt cgcgagctcg acccggtcat
     2941 caatatgacg cttctcaagc tgacaggcgg agggtccata agctgacaag cgattccgca
     3001 cccaagcgta accatccgta aatacattgg tttgatccca aggtccattg actttccatt
     3061 tcttcaggat tataggcttt caaggtgtgc cgaagagaga tgccttgcgt ttctccatag
     3121 tctttttgca tggcaggaga ccatgaaagg aggaaagatc atgaaatacg ggatggcttg
     3181 ttgtctactg atgttgacgg tactcatcgg gccagaagtg gccgtcgccg gggtcatgga
     3241 taaggatttc gaggtcggga caacccggga acttgtcaat ctctgttccg tggccgcagg
     3301 ggacccgatg gcaaaggagg cgatacattt ctgccagggc tacattgtgg gagctttcga
     3361 ttatcacgtg gcacagtccg cgggtcccgg tggcaagtgc ctggtctgca tgcccgatcc
     3421 gccccccgac agagacaaag tggtcgccat gttcgtcgca tgggccaggg agcacccgca
     3481 gtatatgggc gataaacccg tggaaacaca attccgcttc cttatcgaga agtggccctg
     3541 tgcgcgttga aggcagggaa ggaggagatc atgcgtaaga cggtcatcac attcatcgtt
     3601 atcatagcct tcttttttgc cgggtgtgcc gggatgtccc aaacccagca gcgaacggtg
     3661 agcgggaca
//
LOCUS       DAOQTR010000191         3666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28656_length_3666_cov_3.861257, whole genome shotgun sequence.
ACCESSION   DAOQTR010000191 DAOQTR010000000
VERSION     DAOQTR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28656_length_3666_cov_3.861257"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKN85_05625"
     CDS             complement(<1..188)
                     /locus_tag="PKN85_05625"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HNT43919.1"
                     /translation="MYFDLNYVIEQVGKEKGIPKETIIEALEEAILSASKKKYGSHLD
                     LEAHYSEELGEIEVFQFKT"
     gene            complement(191..661)
                     /gene="rimP"
                     /locus_tag="PKN85_05630"
     CDS             complement(191..661)
                     /gene="rimP"
                     /locus_tag="PKN85_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNT43920.1"
                     /translation="MISATETIEKIAGIVAPILGERLLELVEVEFRPAGKRWLLRVYI
                     DREGGVTIDDCEYVSRELSRVLDVEDPIEHPYTLEVSSPGLTRPLKRREDFVRSRGRK
                     CRIITREEIGGKREFLGRIVDTAEDEVTIEEKIGVFTIPICAIKKAHLEFDFEG"
     gene            828..1835
                     /locus_tag="PKN85_05635"
     CDS             828..1835
                     /locus_tag="PKN85_05635"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT43921.1"
                     /translation="MHTYHTYTNPRRILVTGGAGFIGSHLCERLLSDGHEVVCLDNFF
                     TGTRENIAHLIGRPGFEVIRHDITQPILLEVDWIFNLACPASPIHYQYNPVKTIKVNV
                     LGTLNVLGLAKRVRARIFQASTSEVYGDPETHPQVESYWGNVNPIGKRSCYDEGKRVA
                     ETLFFDYMRQNKVDIKVARIFNTYGPRMRADDGRVVSNFIVQALKGSPVTIYGDGSQT
                     RSFTYVDDMLEGIIRMMDHQAEGWKRPEDYTEPMLSGFPGPVNLGNPRESSILDIART
                     VIRLSGSSSPIVHRDLPEDDPKQRCPDISLARKTLEWEPTTGLTEGLTRTIEHFRKAA
                     T"
     gene            1832..2077
                     /locus_tag="PKN85_05640"
     CDS             1832..2077
                     /locus_tag="PKN85_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43922.1"
                     /translation="MTREEELVKAGWEKRFVACEPRLSEMADMYREIGFEVHLEPLPA
                     KEDLGAEETSSCEDKGCTACFDADPERYRIIFTRRPG"
     gene            complement(2345..2614)
                     /locus_tag="PKN85_05645"
     CDS             complement(2345..2614)
                     /locus_tag="PKN85_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43923.1"
                     /translation="MKCRKCKTPITAEKISFRDECPVCGEDLHVCLNCLFYDTGKANS
                     CREDKAEFVKEKDRANFCEYFRFTERQIETSAKEEAEKRWKELFK"
     gene            complement(2616..3254)
                     /locus_tag="PKN85_05650"
     CDS             complement(2616..3254)
                     /locus_tag="PKN85_05650"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT43924.1"
                     /translation="MKKVISFDLDGTLVDARYGDMVWNVGIPSEYAKKYGMAFDEARA
                     FIRARYESVGDGDITWYEIEHWLERFSLDVSPAELLDRYAEHIALLPGAREVVDTLGD
                     RYTLVIASNAARIFVEKELGETGLSGSFSLVVSATSDYGMVKKQEEFFLRLLAEVGAV
                     PHEVVHVGDHPVFDHDVPSGLGIECYCIDGHDGQGNRPRRTISGLKDLLEVL"
     gene            3376..>3666
                     /locus_tag="PKN85_05655"
     CDS             3376..>3666
                     /locus_tag="PKN85_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT43925.1"
                     /translation="MKTMLCVMTALCLFLCIGGNAGAKDTVKIGLITPLTGDVKTYGE
                     SVKNAFTIAVEEYGRKGKYTIQTVMADDRNDATEGANAALKLITQDKVTAIVG"
BASE COUNT          816 a         1139 c          952 g          759 t
ORIGIN      
        1 gtcttgaact gaaagacctc gatctccccc agttcctcgc tgtaatgcgc ctcgagatcg
       61 agatgactgc cgtatttctt cttcgacgcc gagagtatgg cctcctcgag agcctcgatg
      121 atggtctcct tcgggatacc cttttccttc cccacctgct caataacgta attgagatcg
      181 aaatacatcg ttacccctca aaatcaaact ccaaatgtgc tttttttata gcacatatcg
      241 ggattgtaaa cacacctatt ttttcctcta tcgtcacttc gtcttccgcc gtatcgacga
      301 tcctcccgag gaattcccgt ttgcctccga tctcctcgcg ggtgattatc cggcactttc
      361 tcccccgtga cctcacgaaa tcctcacgtc tcttcagggg ccgtgtgaga ccgggagacg
      421 agacctccag ggtataaggg tgctcgatgg ggtcctcgac atcgaggacc cttgacaatt
      481 cgcggctcac gtactcgcaa tcgtcgatgg tcacgccccc ttccctgtcg atgtagaccc
      541 tgaggagcca tcttttgccc gcgggccgga actcaacctc aaccagctcc agaagccgct
      601 cgccaaggat gggtgcaact atgcctgcta tcttttctat ggtttccgtg gcggatatca
      661 tcctggcaat aaattaaaaa agcgggcgga acccacttga cgttactgtc ttatcacatt
      721 tcacggggga atgcaacgac aaataccagc atttatattg actttgcccc gtcctgcggg
      781 gtaaaaccag agtgacgctc cccaaccgac aggcacaggc ggaccgcatg cacacctacc
      841 acacttacac aaatcccagg cgcatactcg tgaccggcgg cgccggtttc atcggctccc
      901 atctgtgtga acggctcctc tccgacgggc acgaggtcgt ctgcctcgac aatttcttca
      961 ccggcacgag ggaaaacatc gcgcacctca tcggccggcc cggtttcgag gtcatccgcc
     1021 acgacatcac ccagcccatc ctcctcgagg tggactggat attcaacctt gcctgcccgg
     1081 cgagcccgat ccattaccag tacaatcccg tgaagaccat caaggtgaac gttctcggga
     1141 cgctcaatgt cctcggtctc gcgaaacggg tccgggcacg catattccag gcctcgacaa
     1201 gcgaggtcta tggagacccc gagacccatc cccaggtcga gagctactgg ggaaacgtca
     1261 atcccattgg aaagagaagc tgctacgacg aaggcaagag ggtcgccgaa accctcttct
     1321 ttgactacat gcgccagaac aaagtggaca tcaaggtcgc gcggatcttc aacacctacg
     1381 ggccgcggat gcgcgccgac gacggtcgcg tcgtgagcaa cttcatcgtc caggccctta
     1441 aggggagccc ggtgaccatc tacggcgacg gttcccagac acggtccttc acgtacgtgg
     1501 atgacatgct cgaaggcatc atccgcatga tggaccacca ggcggaaggc tggaagagac
     1561 ccgaagacta cacggaaccc atgctttccg gctttcccgg ccccgtgaac ctcggcaacc
     1621 cccgggaaag ctcgatcctc gacattgccc gcaccgtcat caggctcagc gggtcatcat
     1681 cgcccatcgt ccacagggac cttcccgagg acgacccgaa gcagcgctgc cccgacatct
     1741 ctctcgcgcg caagaccctt gaatgggagc cgacgacggg actcacggaa ggcctcacgc
     1801 ggacgataga acacttcagg aaggctgcaa cgtgacacgg gaagaggaac tcgtaaaggc
     1861 aggatgggag aaaaggttcg tcgcctgcga gccccggctc tccgagatgg ccgacatgta
     1921 cagggagatc ggcttcgagg tccacctgga gcccctcccg gcaaaagagg acctcggggc
     1981 ggaagagacg tcctcatgcg aagacaaggg ctgcaccgcc tgcttcgatg ccgacccgga
     2041 gcgctaccgc atcatcttca cccggcgccc gggttaacaa tcccctcctc ctgctgtccg
     2101 ggaaatccct tttttccatt gcgtcatcgg gcgcaacatc gttgccggac ggtcaggtaa
     2161 tgaccaacga ccaaattcca ataaccagat aaacagacaa taaccaataa caaaaaaacg
     2221 attcaggcag tcccctgtgc ccttcccctt gttcaattgg ttattgataa attggtcatt
     2281 atcttcttca attggtcatt ggaatttggt tattggctat tacccctccg gtagaccacc
     2341 tgcgtcactt gaacagttcc ttccaccgtt tctccgcctc ttccttcgcg gacgtttcga
     2401 tctgtctttc ggtgaaacgg aagtattcgc agaaattcgc cctatctttt tccttcacga
     2461 actccgcctt gtcctccctg caggaattgg ccttccccgt atcatagaag agacagttca
     2521 ggcagacgtg caggtcctct ccgcaaacgg ggcactcgtc ccggaaagag atcttttcgg
     2581 cggtgatggg ggtcttgcac tttcggcact tcatttcaga gaacctccag gaggtctttg
     2641 aggcccgata tggtcctgcg gggccggttc ccctgcccgt catggccgtc tatgcagtag
     2701 cattcgatcc cgagaccgga agggacatca tggtcaaaaa caggatggtc tcccacgtgg
     2761 acgacctcgt gcgggacggc gccgacctcc gcgaggagtc tcaggaagaa ttcctcctgc
     2821 ttcttcacca tcccgtagtc cgacgtggcc gacacgacga gggagaagga ccccgaaaga
     2881 ccggtctcgc cgagttcctt ctcgacgaat atccgtgccg cgttggaggc gatcaccagg
     2941 gtgtaccggt ccccgagggt atccaccacc tcacgggcac ccggaaggag ggcgatgtgc
     3001 tccgcgtacc ggtcgaggag ctccgccggg gagacatcga gggagaacct ctccaaccag
     3061 tgctctatct cgtaccacgt gatgtccccg tcgcccacgg actcgtaacg ggcccgtatg
     3121 aaggccctgg cctcgtcgaa ggccatgccg tacttcttcg cgtattcgga agggatgcca
     3181 acgttccaga ccatgtcgcc atagcgcgcg tcgacgaggg tcccgtcaag atcgaaggag
     3241 atgacctttt tcatcacagc attatatcag attccggctc ctttttcccc gggttgggcc
     3301 ggttttcgaa aaacattgca ttaagcaggc aagtttgttt taaatttaca cacgaagact
     3361 gacgggagga aaacgatgaa gaccatgctc tgcgttatga cagcactatg cctgttcctt
     3421 tgcatcggcg ggaacgccgg tgctaaggac accgtgaaga tcggcctcat cacgccgctc
     3481 acgggtgacg tgaagaccta cggcgaatcc gtcaagaacg cgttcaccat cgccgtcgag
     3541 gagtatggca ggaaagggaa atacaccatc cagacggtca tggcggatga tcgcaacgac
     3601 gcgacggaag gagccaacgc tgccctcaag ctcatcaccc aggacaaggt gacggcgatc
     3661 gtcggc
//
LOCUS       DAOQTR010000192         3663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28693_length_3663_cov_3.461197, whole genome shotgun sequence.
ACCESSION   DAOQTR010000192 DAOQTR010000000
VERSION     DAOQTR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28693_length_3663_cov_3.461197"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1230)
                     /locus_tag="PKN85_05660"
     CDS             complement(232..1230)
                     /locus_tag="PKN85_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNT43926.1"
                     /translation="MIQHAPYGSLMSDASAFTKSRVILSAVELDIFTLLDGTAETSAA
                     LARRAGFNTRALSHLLDALASLGFLAKNGGSYSLTESGALLSSRHTGSVRPMVLHMGR
                     LWDTWGDLSRVVRKGLKGKRRHASRMDPETLAAFIGAMDVIGRDLSLEIADSYDLGPY
                     RRLLDVGGASGTYTAAFLRKNPLLRATIFDLGVVIPMAREKIASEGLSQRVDLVPGDF
                     YADDLPGGHDLVLLSAIIHQNDPGQNVALYRKAIEALVPGGAILIRDHIMDETRTAPS
                     AGALFAINMLVNTRGGSTYTFEEVKEGLAEAGFEDVELLRTGDRMDCLVEGRKPKR"
     gene            complement(1260..2831)
                     /locus_tag="PKN85_05665"
     CDS             complement(1260..2831)
                     /locus_tag="PKN85_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43927.1"
                     /translation="MKNGVIVVGAGLAGMMAAIAAADEGAEVTLVDRGAIGLGTNTAM
                     SNAVFAGVSDSYGVQEYVRETMEIGRFLNNRKKVETAAEKSREAVALLKSLGVPVGLV
                     GTLYQVRSPDPRVISGVTLVRRVAEAIGSRPSIRVIRDLYVTGIVKRDGVVEGIEGVD
                     SRGGSIVCRADAVVLATGGAGAMYLRNDNQKYIMGQGYHLAAAVGLPLLDMEFVQFFP
                     VVVSGEGLPATIVYPPVHEAIRLVNSAGEDLAVKYGLGSLTDAVMKKRDELSIELYRE
                     TRKGTVFLDCRKVPGEAWNEHPLSILKLIKHDFATRPLPVAPAVHFVMGGVMTDGTCR
                     SGLPGLFACGEVTWGLHGANRRGGNALMECAVFGTLAGRNAAHFNEGGPKGVKMETTE
                     PGTGGTVAAGGSRRSLGDIKGEIRQIAWDYAGVIRSAEGLTKGLEAAREVHRELDGFV
                     PGTRRETCAKEDLTAAVVTLKAILTASLARRESRGSFFREDHPGEDDANWKKNSSLTY
                     DPVERAFAVTHVAVC"
     gene            complement(2914..3438)
                     /locus_tag="PKN85_05670"
     CDS             complement(2914..3438)
                     /locus_tag="PKN85_05670"
                     /inference="COORDINATES: protein motif:HMM:NF020395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family c-type lysozyme inhibitor"
                     /protein_id="HNT43928.1"
                     /translation="MRKIMIPMAVAVVVLVLGVMDVHGQTPKKQSVGKPKSGKSQIVI
                     EFVSFPDQYKRYEVYPWETLKVDDFQEAYTAMTQEGKFEDWVKSLSGTAQGKNRMIRV
                     SRESFVLVVCCKPHMCDTSQIIVLYDPVKKKTFSMLAVDGKFSWFGKPPEHIKDLLNV
                     LLVEEFRAIYRGQI"
     gene            complement(3459..>3663)
                     /locus_tag="PKN85_05675"
     CDS             complement(3459..>3663)
                     /locus_tag="PKN85_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNT43929.1"
                     /translation="SSDNRLSFSFRLVMAPYDVIDYIVVHELAHIKIKNHSKRFWEYI
                     GKVMPDYGRRKEWLAKKGYLLDI"
BASE COUNT          675 a         1211 c         1008 g          769 t
ORIGIN      
        1 tctcctccat gccccatacc acctcacccc cccacacctt cttatccctt ccccccatca
       61 ctcccgtctc aagtcaaagc ccttcctttc cctcccgtcg cagacgggat gcaaagacgg
      121 ccggatcccg gcgggcacct ggatccggcc gcatcttttc tctgaacgct ctcttcatca
      181 cctccgcgcc ctcgagtccc gtcaacgacg ggacgggcgt gagataagat cctatctttt
      241 cggtttcctc ccctctacca gacagtccat cctgtcaccc gtgcgcagaa gctcgacgtc
      301 ctcaaagcct gcctccgcca gaccctcttt cacctcttcg aaggtatagg tgctgccgcc
      361 acgggtgttc acgagcatgt tgatggcgaa gagcgccccc gcggacggcg cggtgcgcgt
      421 ctcgtccatg atatgatcgc ggatcagtat ggccccgccg gggacaaggg cttctatggc
      481 cttgcggtat agagcgacgt tctgtccggg atcgttctgg tggattatgg ctgaaagcag
      541 gacgaggtcg tgccctcccg gcaggtcgtc cgcgtagaag tcaccgggaa cgaggtccac
      601 tctctgcgag aggccctccg aggctatctt ctcccgggcc atgggtatga cgaccccgag
      661 gtcgaagatc gtggcccgca ggagcgggtt cttccgcagg aaggctgccg tgtacgtccc
      721 cgatgcccct cccacgtcga gaagcctcct gtacggccca aggtcatagg agtccgctat
      781 ttccagggac aggtctctgc ctatcacatc catggcaccg atgaaggccg ccagggtttc
      841 ggggtccatc cggcttgcgt gcctgcgctt tcccttgagc cctttcctca ccacgcgcga
      901 aaggtctccc caggtgtccc agagcctgcc catgtggagc accatgggac gtaccgaccc
      961 ggtatgacgc gaagacagga gcgcaccgct ctccgtgagg gagtaggacc cgccgttctt
     1021 cgcgagaaaa cccagggagg caagggcatc gagaaggtgt gagagcgccc gggtgttgaa
     1081 tcccgccctc ctggccagag cggccgaggt ctcggccgtc ccgtcgagaa gagtgaaaat
     1141 atcgagctcc accgcggaaa ggatcaccct gctcttcgtg aaagcgctgg cgtcggacat
     1201 cagtgaaccg tatggtgcat gctggatcat ggcagtctcc ttgcgatagt gtatggtagt
     1261 caacagacgg cgacgtgggt caccgcgaag gccctctcca cggggtcata ggtcagggaa
     1321 gagttctttt tccagttggc atcatcctcg ccgggatggt cctcgcggaa aaagctgccg
     1381 cgactctccc gccttgcaag gctcgccgtc aggatcgcct tcagggtcac gaccgccgcc
     1441 gtcaggtctt ccttcgcaca tgtctcgcgt ctcgtaccgg gcacgaaccc gtccagttcc
     1501 cgatggacct cgcgggcggc ttcaaggcct ttcgtcaatc cttccgccga gcgtatcacg
     1561 cccgcgtagt cccaggctat ctgccgtatc tctcccttta tgtcgccgag ggaccttcga
     1621 gatcctccgg cggccacggt ccctcctgtg ccgggctccg tggtctccat cttcacccct
     1681 ttcggacccc cctcgttgaa atgggcggca ttccgtcccg ccagcgtccc aaagacggcg
     1741 cactccataa gggcgttccc gccgcgcctg ttggcgccgt ggaggcccca cgtcacctcc
     1801 ccgcaggcga agagccccgg cagtccggat cgacaggtgc cgtcggtcat cacgcctccc
     1861 ataacgaaat gcaccgccgg agcaacaggg agaggcctgg tcgcaaaatc gtgcttgatg
     1921 agcttcagga tgctcaaggg gtgctcgttc cacgcttccc cgggcacctt tcggcaatcg
     1981 aggaacacgg ttcctttccg ggtctcccgg tagagctcta tggaaagttc gtcacgcttc
     2041 ttcatgacgg cgtcggtcag gctgccgaga ccgtacttga cggccaggtc ctccccggcg
     2101 ctgttgacca gtcttatggc ttcgtggacg ggcggataga cgatggtggc cggcaggccc
     2161 tctcctgaaa caacgacagg gaagaactgc acaaattcca tgtcgagcaa gggaaggccg
     2221 acggccgcgg caaggtggta tccctgtccc atgatgtatt tctggttgtc attcctgaga
     2281 tacatggccc cggcgccgcc cgttgccagg acaacagcat ccgcgcggca gacaatgctc
     2341 ccgccacggg agtccacacc ttctatgccc tccaccacac catcccgctt cacgataccg
     2401 gtaacataga ggtcgcgaat gacccggatg ctcggacggg aacctatcgc ctcggcaacc
     2461 cttctcacca gcgtcactcc cgagatgacc cggggatccg gtgatctcac ctggtagagg
     2521 gtccccacga gacccacggg gaccccaagg gacttcagca acgcgacagc ctcgcggctc
     2581 ttttccgccg cggtctcgac cttcttcctg ttgttgagga aacggcctat ctccatcgtt
     2641 tcccggacgt actcctgtac cccgtacgag tcggaaacac ccgcgaagac cgcgttcgac
     2701 atggccgtgt tcgttccgag tcctatggcg ccccggtcta cgagggtcac ttccgcgccc
     2761 tcatccgcgg ccgcgatggc cgccatcatt ccggcaagcc ccgctccaac gacgataact
     2821 ccgttcttca tgttccactt ccttccttgt tcgatgcggc attatgacat atcgatattt
     2881 ggccgtggaa gaagggtact caggggaagc gcttcagatc tgacccctgt atatggccct
     2941 gaactcctcc acgaggagca cgttgaggag gtcctttatg tgctcgggag gctttccgaa
     3001 ccatgagaac ttgccgtcga cggcaagcat gctgaaggtc ttcttcttca cgggatcgta
     3061 aaggacgatg atctggctcg tgtcacacat gtggggcttg cagcacacaa cgagcacgaa
     3121 gctctccctc gatacccgta tcatccggtt cttgccctgg gcggtgcccg agagggactt
     3181 tacccaatcc tcgaactttc cctcctgggt catcgccgtg taggcctcct gaaaatcgtc
     3241 caccttgagc gtctcccacg ggtagacctc atacctcttg tactggtcgg ggaaggagac
     3301 aaattcgatc acgatctgcg actttccgct cttgggcttt ccaacggatt gctttttcgg
     3361 cgtctggcca tggacgtcca tgacaccgag aacaaggacg acaacggcta ccgccatcgg
     3421 gatcatgatc tttctcatcg cgtctcctcg ccggaccgct atatgtccag cagataaccc
     3481 tttttcgcaa gccattcctt gcgcctcccg tagtccggca tcaccttccc gatgtactcc
     3541 cagaaccgct tcgaatggtt tttgatcttt atgtgcgcaa gttcatggac cacgatatag
     3601 tcgatgacgt catagggggc catgacaagt cgaaagctga aggagagtct gttgtcggag
     3661 gag
//
LOCUS       DAOQTR010000193         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_28713_length_3662_cov_2.871638, whole genome shotgun sequence.
ACCESSION   DAOQTR010000193 DAOQTR010000000
VERSION     DAOQTR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28713_length_3662_cov_2.871638"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKN85_05680"
     CDS             complement(<1..754)
                     /locus_tag="PKN85_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT43930.1"
                     /translation="MDKIDAIVVGGGLAGLSAAYRLADAGKQVILLERGDAPGSKNVT
                     GGRIYVDPIRSYLPGVIDSAPFERHVVREMITVLDEGSGLQFEYGNEKWKKEPFMSHT
                     VLRAKFDSWLADQAGAKGAFIIPRKKVDELLYEDGRVAGIRSGSEEIGAHIVIAADGA
                     LSFLAEKAGLRKPHRPENLAVAVKEIYQLDERTIEERFGLGPDEGVAGLFMGSVTRGM
                     FGGGFLYTNRDTLSVGLVVGIDSLIHGAGGFDA"
     gene            complement(765..1745)
                     /locus_tag="PKN85_05685"
     CDS             complement(765..1745)
                     /locus_tag="PKN85_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNT43931.1"
                     /translation="MGKVLVFVETKGDEPRKASLELLCEAEKLAASGKFSVEAVCFGA
                     MSDVLKGRLLSCVRRLVRFTDPELAAYSPEGYARALAGYAAETGARMIVAGATGIGRD
                     FLPRVAVMLGTGMVSDVTGANWFDEPMTFVRPMFGGKVFAEVAFPASPVVVTARPNSF
                     AMDIPEGVNGEYEEKAAGSLGGAIHTRVVRVEETKGDAVDLTEADLIVAGGRGLKAAE
                     NFKLIEDLAAAIGGTVGATRSVVDAKWRDQADQIGKSGKTVSPKLYIGAGISGAIHHI
                     MGMDTSKVVLAVNRDPNAIIFNYANYGIVGDLFEVLPAMTEEIRKRKERG"
     gene            complement(1759..2541)
                     /locus_tag="PKN85_05690"
     CDS             complement(1759..2541)
                     /locus_tag="PKN85_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNT43932.1"
                     /translation="MDIIVLVKQVPDPEALVEIGASGKDLNIEPKFAVNLFDEFALEE
                     ALRIKEKHGGKVKALSLGGPKAMEALRTAIAMGADEAVLIEDDAYAARDGYSTALALF
                     KALEKETFDIILTGRQAIDADRGEVPQMVSQMLGLPHVGVVVKLDISDGKAEAVSSLE
                     GAKEVVEVALPAVFTAQKGLNEPRVPLITGVMKAMKVQIPKLTIEDLGLTKDMAAPEN
                     SKTQIVRYLPPKKRPAVQLIPGEAREAAAEAVRILVDIERVI"
     gene            complement(2584..>3662)
                     /locus_tag="PKN85_05695"
     CDS             complement(2584..>3662)
                     /locus_tag="PKN85_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT43933.1"
                     /translation="VVPRAREIDEKDEFPWELWKEFTRLGYLGLRYPEEIGGMNADPV
                     TAMIFYEQIARGSVGFAQSVIMNILMGTYFVYRFGSQAIKERCLYPAMRGEKIATMCF
                     TEDQSGSDLAATRTTAVKDGNEWVINGTKMWITNGPHADIATVLATTDPSLGAKGLNF
                     FLVEKGTPGFSPGQILDKLGCRGTVTGELVFDNVRIPEENLLGAELNKGVEYLAEILD
                     EVRVMTAAMAMGIAQAAYDEGLEYAKKRIAFGKPIGTYQLIRAKFADMATEMEASRLL
                     IYSAAWKIKEKLPSRLEAAMAKMFATETCLKVVDEVTRIWGANAFANEYNPQRHFRDA
                     RFLLYGGGTHEILKDFMGRMLIGK"
BASE COUNT          652 a         1241 c          990 g          779 t
ORIGIN      
        1 gagcgtcgaa gccgcccgcg ccgtgtatga gggaatcgat accgacaacg aggcccacgg
       61 agagagtatc gcggttcgtg tagagaaaac ccccaccgaa catgccccgc gtcacggaac
      121 ccatgaaaag tccagccaca ccctcatcgg gaccgagccc gaaccgttcc tcgatggtcc
      181 tttcatcgag ctggtatatc tctttcaccg ccactgccaa attctcaggc cggtgcggtt
      241 ttctcaaacc cgccttctct gcaaggaacg acaacgcgcc gtcggcggct atgacgatat
      301 gggcccctat ctcctcggaa ccggaccgta tccccgccac acgtccgtcc tcgtaaagga
      361 gctcgtccac cttcttcctc gggatgatga aggctccctt tgcccccgcc tgatcggcaa
      421 gccagctgtc gaactttgcc ctgagaacgg tatggctcat gaagggctct ttcttccatt
      481 tctcgttccc gtattcgaac tgcaggccgc tgccttcgtc gagaacggtg atcatctccc
      541 tgacgacgtg gcgttcgaag ggcgcggaat ctatgacccc gggcagatag gaccggatgg
      601 ggtcgacgta gatgcggccc cccgtcacat tcttgcttcc cggagcatcg ccgcgttcga
      661 ggagtatcac ctgcttgccg gcatccgcga ggcggtacgc cgcggacagc cccgcgagcc
      721 ctcctcccac aacaatggcg tcgatcttat ccacgaggaa tcacctatcc cctttctttg
      781 cgcttcctga tctcttccgt catcgccggg agcacctcga agaggtcgcc gacgataccg
      841 tagttggcat agttgaagat gatcgcgttg gggtcgcggt tgaccgcaag gacgaccttc
      901 gatgtgtcca tgcccatgat gtggtggatg gcccccgaga tgcccgcccc gatatagagc
      961 ttcggggaca ccgtcttgcc gctcttccct atctggtccg cctggtcccg ccacttcgcg
     1021 tcgacgacgg accgtgtggc tccgaccgtg cctcctattg ccgcagccag gtcctcgata
     1081 agcttgaagt tctccgctgc cttcagcccc cgtccgcctg ccacgatgag gtcggcctcg
     1141 gtgaggtcca cggcgtcgcc ttttgtctcc tcgacgcgaa caacgcgggt atggatggca
     1201 ccccccaggg atcccgcggc cttctcctcg tattccccgt tgaccccttc gggtatatcc
     1261 atggcaaagc tgttggggcg cgccgtcacc accacgggag acgccgggaa ggcgacctcc
     1321 gcaaagacct tccccccgaa catgggcctg acgaaggtca ttggctcgtc gaaccagttc
     1381 gcccccgtca cgtcggaaac catgcccgtc ccaagcataa cggcaacacg agggaggaaa
     1441 tcgcggccta tccccgtcgc cccggccacg atcatccgcg cgcccgtctc ggcggcatag
     1501 ccggccagcg cccgggcgta gccctccggg ctgtacgccg caagttcggg gtcggtgaag
     1561 cggaccagtc tgcgcacgca ggacaggagc cttcccttga gaacatcgga catcgcgccg
     1621 aaacacactg cctccacgct gaacttgccg cttgcggcca gcttctccgc ttcgcagaga
     1681 agttccaggg acgcctttct gggttcatca cccttcgttt ccacgaacac aaggaccttg
     1741 cccatctttc cccctgtctt atatgaccct ctcgatgtcg acgaggatcc tcaccgcctc
     1801 ggccgcggcc tcccgggcct ctcccgggat gagctgaacc gccggccgtt tcttcggcgg
     1861 caaatacctc actatctgcg tctttgaatt ctccggagcg gccatgtcct tcgtgagccc
     1921 caggtcctcg atagtgagct tcggtatctg caccttcatg gccttcatga cacccgtgat
     1981 caagggtacc ctgggctcgt tgagaccttt ttgcgccgtg aagacggcgg gaagagcaac
     2041 ctcgacgacc tctttcgcgc cttcaagaga cgacaccgcc tcggccttgc cgtcggaaat
     2101 gtccagcttg acaacaacgc ccacgtgggg cagacccaac atctgggaca ccatctgggg
     2161 cacctcgccg cggtccgcat cgatggcttg cctgccagtg aggatgatgt cgaaagtttc
     2221 cttttcaagg gccttgaaaa gggcaagagc cgtgctgtaa ccgtcccttg ccgcataggc
     2281 gtcatcctct atgagcacgg cctcatcggc gcccatggcg atggctgtgc gcagggcctc
     2341 catggccttc ggacccccga gagacagggc cttcaccttt cccccgtgct tctcctttat
     2401 cctgagcgcc tcttcgagag caaactcatc gaagaggttg accgcgaact tgggctcgat
     2461 attgaggtct ttgccgctcg cccctatctc gacgagcgcc tcaggatcgg gaacctgctt
     2521 cacgagaact atgatatcca ttgtatctca cctcgcatcc cgttatccgg gcaaacacat
     2581 ccgctacttg ccgataagca tcctgcccat gaaatccttg aggatctcgt gtgtgccgcc
     2641 tccataaagg aggaagcgag catccctgaa atgccgctgg ggattgtact cgttggcgaa
     2701 ggcgttggca ccccagatcc tcgtcacctc gtccacgacc ttgaggcacg tctccgttgc
     2761 gaacatcttc gccatggccg cctcgagacg gctcggcaac ttttccttga tcttccacgc
     2821 ggccgagtag atgagaagcc tgctcgcctc catctccgtt gccatgtccg cgaatttcgc
     2881 cctgatgagc tgataggtac ctattggctt tccaaaggct atccttttct ttgcgtactc
     2941 gaggccctcg tcgtatgccg cctgggctat gcccatggcc atggcggccg tcatgaccct
     3001 cacctcgtcg aggatctccg cgagatactc gacgcccttg ttgagctccg cgcccagcag
     3061 gttctcttcc ggtatcctca cgttgtcgaa gaccagttca cccgttaccg ttccccggca
     3121 gccgagcttg tcgaggatct gcccgggtga gaatccgggg gtccctttct cgacaaggaa
     3181 gaaattgagg cccttggctc caagcgacgg gtccgtcgtc gccagcaccg tcgcgatgtc
     3241 ggcatgggga ccgttggtga tccacatctt cgtgccgttt atgacccact cgttcccgtc
     3301 cttgactgcc gtcgtccttg tggcggccag gtcggagccc gactggtcct ccgtgaaaca
     3361 catggtcgcg atcttttccc ctctcatcgc ggggtagaga caccgctcct ttatggcctg
     3421 tgaaccgaaa cggtatacga agtaggtgcc catgaggatg ttcatgatga cgctctgggc
     3481 gaatccgacc gaaccgcgcg cgatctgctc gtaaaagatc attgccgtca ccgggtcggc
     3541 gttcatgccg cctatctcct cggggtagcg caggccgaga taccccagcc tcgtgaactc
     3601 cttccagagc tcccagggga actcgtcctt ctcgtcgatc tcgcgggccc tgggcacgac
     3661 ca
//
LOCUS       DAOQTR010000194         3620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29172_length_3620_cov_3.364376, whole genome shotgun sequence.
ACCESSION   DAOQTR010000194 DAOQTR010000000
VERSION     DAOQTR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29172_length_3620_cov_3.364376"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1013
                     /locus_tag="PKN85_05700"
     CDS             228..1013
                     /locus_tag="PKN85_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNT43934.1"
                     /translation="MKIFHVDAFTPEPFSGNPAGVCILSHPKVDAWMQRVAAEMNLSE
                     TAFLIRAADGYSLRWFTPMVEVDLCGHGTLASAHILWQEGLAARDEKITFHTRSGVLT
                     CTQDGEWVGLDFPATPDVEVAPPEGIAEALGAVPLYVGVSRSDYIIEVDSEETVRGLT
                     PDYDKILGLPVRGVIVTSRATSDEFDFVSRFFAPAVGVNEDPVTGSAHCCLGPFWGRR
                     LGRESLIARQVSRRGGVVKVRLAGDRVHLFGQAVTVMKGEMVV"
     gene            complement(1244..2248)
                     /locus_tag="PKN85_05705"
     CDS             complement(1244..2248)
                     /locus_tag="PKN85_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HNT43935.1"
                     /translation="MDRKRKKDLLILGILRNSSTPLTSTKIAQELQSLGHDISERTVR
                     LYLQRMDTEGLARQARKKGHEITARGESELDSSRIIDRVGFLSAKIDRMTYQMSFDLN
                     TTSGSVVINVTIVDPRQLAKNVDYITRVYADGYAMGHLMAFLSPGETLGHISIPEGMI
                     GVGTVCSITLNGVLLKHGIPTVSRFGGLLEIEDKKPVRFVEIIMYDGTSIDPLEVFIR
                     SGMTNYMGAVDTGSGRIGASFREFPAESRDLVEQLAGKLKRIGLGGLVRIGLPGQPLL
                     DIPVNEGRVGAIVIGGLNPVSILEETGVRAYSRALAGLIDFTRLFRYDEMESRIKEYL
                     "
     gene            2485..>3620
                     /locus_tag="PKN85_05710"
     CDS             2485..>3620
                     /locus_tag="PKN85_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNT43936.1"
                     /translation="MIEWSKTNDAIGTVNRGNPAESGLCTLCRADCKGRCETFLSSLK
                     GRKILYPRDFGIVTAGSANTTHVGVNYNALRIQGYLYGVRGLPKGLKNTADDCIFPNV
                     SIESEFGSKVITKTKMPIMTGALGSTFIAAKYWDSFATGAALVGIPIVVGENVVGIDK
                     EAVINNGKIEKAPELDRRIDTFLKYYDGYGAIIVQMNVEDTRNGVAEYVIDKYGDKVI
                     IELKWGQGAKDIGGEIQVDSLDYALFLKNRGYIVDPDPTIPEVQEAFKHGAIKSFARH
                     SRLGYTDLSTPEKVRENFMRSVNYLRKLGYRRISLKTGSYGMEALAMSLKFATDAKLD
                     LLTIDGSGGGTGMSPWNMMESWGVPSLLLHSKAYEYATIIAAQG"
BASE COUNT          781 a          982 c         1078 g          779 t
ORIGIN      
        1 atgataaggg tggagatgat gatccttgtg atcattttca ttgcgcaaac ccctttgtcg
       61 caacgttcag tgccttgcgg ccagtcctga cagagttata tgcaaaagaa ggggaattgt
      121 caataagtac ggccctttta gtgttgacaa ccaggtctcg gaagtgcaga atctcgaagg
      181 ggccttcaaa ccaacctgtc gatacttcac ggaaagagga gtgtgtcatg aagatctttc
      241 acgtcgatgc cttcacgccc gaaccgttca gcggcaatcc cgcgggggtc tgcattctct
      301 cccatcccaa ggtggatgcc tggatgcagc gcgtggccgc ggagatgaac ctttcggaaa
      361 cggctttcct tatccgcgcg gccgacgggt acagcctgag atggttcacc ccgatggtag
      421 aggttgacct gtgcggacac gggaccctgg cgagcgcgca catcctgtgg caggaaggtc
      481 tggcagcgag ggatgagaag atcacctttc acacacgaag cggcgtgttg acctgcacac
      541 aggacgggga atgggtcggc ctcgattttc cagcgacccc cgatgttgag gtcgctcccc
      601 cggagggtat cgcggaggcg cttggcgctg tcccgttgta cgtgggcgtg agccgctccg
      661 attatattat agaggtggat tccgaagaga cggtgcgagg cctgaccccg gactatgaca
      721 agatactcgg gctgcccgtc cgcggggtga tcgtgaccag cagggcgacc tcggatgagt
      781 tcgatttcgt gtcacgtttc ttcgcccctg ccgttggcgt caatgaagat cccgtcaccg
      841 gttcggccca ttgctgcctg gggcccttct ggggcaggcg tcttggcagg gaaagcctca
      901 tcgcccggca ggtatcacgt cgcggaggcg tggtaaaggt ccgcctcgcc ggtgaccgtg
      961 tccatctctt cggccaggcg gttacggtga tgaaagggga gatggtggtg tagagtgaga
     1021 acgggtaatg ggctataggt tataggtata ggttatagaa aggatggggc caattagggc
     1081 ggactagggt ctcttaactc ttttgtgcgg atcgcgaagg cgctgtgtgg caaaaatgcc
     1141 gggagtcgaa ccggcaaaat tgccgaaaag aatcccggtc acgggaagga gttgtttttt
     1201 gttcctgcct atcacccatc acctatgacc catcacctga tctttacagg tattccttta
     1261 tcctgctttc catctcgtcg tagcggaaga gcctcgtgaa atcgatgagc cccgcaagcg
     1321 cgcgggaata ggcccttacg ccggtttctt cgagaatgga gacggggttc aggccgccta
     1381 tcacgatggc cccgaccctg ccttcattca cagggatgtc gaggagtggc tgcccgggca
     1441 ggccgatgcg gacaaggccc ccgaggccta tcctcttgag ttttcccgca agctgttcga
     1501 cgaggtccct gctctcggcg gggaactcgc ggaaactggc gccgatcctg ccgcttcccg
     1561 tgtcgaccgc tcccatgtag tttgtcatcc cgctgcggat aaacacctcc aggggatcga
     1621 tgctcgttcc gtcatacatg atgatctcca cgaaacggac cggcttcttg tcctcgatct
     1681 cgagaagccc tccgaagcgc gagacggtgg ggatcccgtg cttgaggaga acgccgttga
     1741 gggttatgga gcataccgtt ccgacaccga tcattccttc gggtatgctg atatgtccca
     1801 gggtctcccc cgggctcagg aaggccatga ggtggcccat cgcatagccg tcggcataca
     1861 ctcgtgttat gtagtccaca ttcttcgcca gctgacgagg gtccacgatg gtcacgttga
     1921 tgacaacgct gcccgatgtc gtattgaggt cgaaggacat ctggtaggtc atgcggtcaa
     1981 tctttgccga gaggaatccc acgcggtcga ttatacggga ggagtcgagc tcgctctctc
     2041 cccgggctgt tatctcgtgg cccttcttgc gtgcctggcg ggccaggccc tccgtatcca
     2101 tcctctgcag gtagagcctg acggtccttt cgctgatatc gtgcccgaga gactgaagtt
     2161 cctgggctat cttcgtactt gtgaggggtg tggaggaatt cctgaggatg cccaggatca
     2221 ggaggtcctt ctttctcttt ctgtccatgt cacgctgctc ctttgcggca aaaccaaatc
     2281 tattagcggc aaatttgccg gtgttgttat gatatccgat gaaagacccc tttgcaagaa
     2341 caatcgcaac gaacccctta attattataa aaatctagtt gacaaggaaa ccgggtgttg
     2401 atattctttg ccgaagttaa gcggcaatgg cttgccggta aagtggcagg aagttaccgt
     2461 agagaataca ttagggaggt tgacatgata gagtggtcca agacgaatga cgcaattgga
     2521 accgtgaacc gtggtaaccc ggcggaatcg gggctgtgca cgctgtgccg ggcggactgc
     2581 aaagggagat gcgaaacgtt cctgtcgagc ctgaagggca ggaagatcct ctacccgaga
     2641 gatttcggta tcgttacggc gggcagcgcg aacacgaccc atgtcggcgt gaactacaac
     2701 gcgctgagga tccagggtta tctctacggt gttcgaggcc tgccgaaggg actcaagaac
     2761 acggcggacg attgcatctt cccgaacgtc agcatcgaat ccgaattcgg gtcgaaggtg
     2821 atcacaaaga cgaaaatgcc tatcatgaca ggcgccctcg gttccacctt catcgctgca
     2881 aaatactggg attccttcgc caccggcgcc gccctcgtcg gcattccgat cgttgtcgga
     2941 gagaacgttg tcggtatcga caaagaggcg gtcatcaaca acggcaagat cgaaaaggcg
     3001 ccggagctcg acaggcgcat cgacacattc ctgaaatact atgacggcta cggagcgatc
     3061 atcgtccaga tgaacgtcga ggacacgcgc aacggcgtgg ccgaatacgt catcgacaag
     3121 tacggcgaca aggtcatcat cgaactgaaa tggggccagg gcgcgaagga catcggcggt
     3181 gagatccagg tggacagcct cgactacgcc ctgttcctca agaacagggg ttatatcgtc
     3241 gaccccgatc cgaccattcc cgaggtccag gaggccttca agcacggtgc catcaaatcc
     3301 tttgcccgcc acagcagact tggttacacg gacctctcca cgcccgaaaa ggtgcgggag
     3361 aacttcatga ggtccgtcaa ctatcttcgc aagctcggat acaggaggat ctcactcaag
     3421 accggttcct acggcatgga ggcccttgcg atgtctctca agttcgcaac ggatgcgaag
     3481 ctcgacctgc tcaccatcga cggttccggc ggagggacag gcatgagccc gtggaacatg
     3541 atggaatcct ggggtgtgcc ctcgcttctg ctacacagca aggcgtatga gtatgccacg
     3601 atcatagcgg cacaggggca
//
LOCUS       DAOQTR010000195         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29225_length_3616_cov_3.474867, whole genome shotgun sequence.
ACCESSION   DAOQTR010000195 DAOQTR010000000
VERSION     DAOQTR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29225_length_3616_cov_3.474867"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PKN85_05715"
     CDS             <1..1038
                     /locus_tag="PKN85_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HNT43937.1"
                     /translation="NVLGVLDRSGSTGNSMQLQPSADGDQGIVEAGFGAVGRFLETTT
                     SRIDQVIAGNVAGSAGTGISTGDETLKWGVWAQGFGSYLKQEPRDATMGYTAHIWGTS
                     MGFDRLVSNHSLFGVSGGFAKAYIRTSDTGTRTDADSYQGNLYASYFRDGWRVDGIVS
                     CAYNRYDGSRHIAFGSVDRLATSNYGGYQYSGYVEGGYTVTGGGWNVTPLVSLRYSRL
                     HLNGYTEDGAGSLNLSVDPRDYNMFQSGLGTSFSHPFRWQGSRLVPELHLKWFYDFIG
                     DRQQVTSTFTGGGASFSTEGYDPPRSSLNAGARLTLINGNGVTVSLNYDLELKKDFYS
                     HAGYANIRFLF"
     gene            1004..1999
                     /locus_tag="PKN85_05720"
     CDS             1004..1999
                     /locus_tag="PKN85_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43938.1"
                     /translation="MPAMRTFASFFKGARLILFWTGLLVALWGCASIGPYGDRYEAAD
                     RVAAAGGLTKVHIQTRDFALTAYARISSPGADITVYIEGDGSAWLSPTLLSGDPTPAR
                     PLVLQMAALDPAGNVAYLARPGQYASGPSPGCDPLYWSDRRFSPEVVEAMSEGIEKLR
                     CLSGSGRVDLVGYSGGGAIAVLVAARRSDIASLRTVAGNLDTEAVNRHHGVSFLAGSL
                     NPIDEAARLARLPQRHFAGAKDTVVPVFVVRSFLKQAGDTDTRRLVVLKDVSHSRGWL
                     EKWGDLLALPPITARQGLLDSHALRQVPGLVDVGAEKVGHVVGHELEGHYGKNGL"
     gene            complement(1883..>3616)
                     /gene="glmS"
                     /locus_tag="PKN85_05725"
     CDS             complement(1883..>3616)
                     /gene="glmS"
                     /locus_tag="PKN85_05725"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNT43939.1"
                     /translation="DSAGIAIWNDGAVRLARRQGKVSDLAEALRDGGFDGSMGLAHTR
                     WATHGVPSERNAHPHTAADITVVHNGIIENYLELKEALKEEGHTFTSDTDTEVIPHLI
                     ASLLGTTGSFEGAVRAALRMLKGSYAVGIIRQGEDTLIAAKKESPLLVGLGDNEYIVA
                     SDAPAIINRTNRFIFLEDDDIAVIQKGVLKITNIDGDEVQREARSVQWSGAMAEKGGY
                     RHFMLKEIFEQPRAISDTLIGRIQQEEGEILFEELDLPDITKVGKIWMVACGTSYHAC
                     LIGKHIFEANLRIPVETDIASEFRYRDPILGDNDLVILVSQSGETADTIAAMKEVKKK
                     GLHTLAICNVLGSTLSRECHSVIYTHAGPEIGVASTKAFTTQVSVFFMLMLYLGKRLG
                     VVDSDDGRRLIGELRKIPHKIQTILDASSSIEEHARENMQYRHFLYLGRGINFPTVLE
                     GALKLKEISYIHAEAYAAGEMKHGPIALIDENMPIVFVSPRDQTYKKTCSNMEEVLAR
                     KGKVLLFTDNSRHDMADRVDALFVVPESIYELQPILTIVPLQLMAYYMANLLGTDVDQ
                     PRNLAKSVTVE"
BASE COUNT          621 a         1074 c         1127 g          794 t
ORIGIN      
        1 aatgtgctgg gcgtgcttga cagatcgggg agcactggca actccatgca gctccagccc
       61 tcggcggatg gcgatcaggg aattgtcgag gccggtttcg gggccgtcgg ccgctttctg
      121 gaaacgacca cgagccgcat cgatcaggtc attgccggga atgtggcggg gagcgcaggg
      181 acgggaatat cgacgggtga cgagaccttg aagtggggag tctgggccca gggcttcggc
      241 agctatctga aacaggagcc ccgcgatgcc accatgggtt acaccgcgca catatggggt
      301 acatcgatgg gtttcgacag gctcgtgagc aaccactccc tcttcggtgt gagcggcggg
      361 ttcgcgaagg cctatatccg gacgagcgac accgggacca ggaccgacgc cgacagttac
      421 cagggaaatc tgtacgcgag ctacttcagg gacggctggc gtgtcgacgg gatcgtctcc
      481 tgcgcgtaca accggtacga cgggtcgagg catatcgctt tcggcagcgt cgaccgcctc
      541 gcgacgagca attacggagg ctaccagtac tccgggtacg tcgagggggg atacaccgtc
      601 acgggcggag ggtggaacgt cacacccctt gtctccctgc gttattcgcg tcttcatctt
      661 aacggctata cggaggatgg ggccggttcg cttaacctgt ctgtcgaccc ccgggactac
      721 aatatgttcc agtccggtct gggaacgagc ttctcgcatc ctttccggtg gcagggctcc
      781 cggctcgtac ctgaactaca cttgaagtgg ttctacgact tcatcggcga ccgccagcag
      841 gtcacgtcca ccttcacggg gggcggagcg tcattctcca ccgagggata cgatccaccc
      901 cggtcgagcc tgaacgctgg agcgcgtctg acactgatca acgggaacgg tgtgacggtg
      961 tctctcaatt atgacctgga actcaagaag gatttttaca gccatgccgg ctatgcgaac
     1021 attcgcttcc ttttttaagg gcgcgcgcct tatcctgttc tggacgggcc tccttgttgc
     1081 gctgtggggc tgcgcctcca tcggacccta tggcgacagg tatgaggcgg cggatcgcgt
     1141 cgcggcggcg ggcggtctca caaaagtcca tatccagacc agggattttg cgctcaccgc
     1201 ttacgcgcgc atctccagcc ccggcgcgga catcacggtg tacatcgagg gggatgggtc
     1261 cgcgtggctc tcaccaaccc tgctctcggg ggacccgacc ccggcgagac ctctcgttct
     1321 gcagatggcc gcattggacc cggccggaaa tgtcgcctac ctcgctcgcc cgggacaata
     1381 cgcttcgggt ccctcgcccg gatgcgatcc cctgtactgg tcggacaggc gcttctctcc
     1441 cgaggtcgtc gaggcgatga gcgaggggat agagaagctt cgttgcctct cgggctcggg
     1501 cagggtcgat ctcgtcgggt attccggggg cggcgccatc gccgtcctcg tcgcggcgcg
     1561 gcgaagcgat atcgcaagcc tgaggactgt cgcgggtaac ctcgacacgg aggcggtgaa
     1621 ccgccatcac ggtgtgagct ttcttgcggg gtcccttaac cccatcgatg aggcggcaag
     1681 gctggcacgt cttccgcagc gccattttgc cggggcgaag gatacggtgg taccggtctt
     1741 cgttgtacgg tctttcctaa aacaggcagg cgataccgac acccgcaggc ttgttgtcct
     1801 caaggatgtt tcccattccc ggggctggct tgagaagtgg ggagaccttc tggcccttcc
     1861 tcccataacc gcccgccagg gactactcga cagtcacgct cttcgccagg ttccggggct
     1921 ggtcgacgtc ggtgccgaga aggttggcca tgtagtaggc catgagctgg aggggcacta
     1981 tggtaagaat gggctgtagc tcatagatgc tctcgggaac gacaaacagg gcgtccaccc
     2041 tgtcggccat gtcgtgccgg gagttgtccg tgaagaggag gacctttcct tttcgtgcca
     2101 gcacctcctc catgttggaa caggtcttct tgtatgtctg gtcccgcggc gatacgaaga
     2161 cgatgggcat gttctcgtct atgagggcta tggggccgtg cttcatttcc ccggccgcgt
     2221 acgcctccgc gtgaatgtag gagatctcct tgagcttcag ggctccctcg aggacggtcg
     2281 ggaagttgat gcccctgccg agatagagaa agtggcggta ctgcatgttc tcccgggcgt
     2341 gctcctcgat cgaggaactg gcgtcgagta tcgtctggat cttgtgcggg atcttcctga
     2401 gttcaccgat gaggcgcctt ccgtcgtcac tgtcgacaac gccgagacgt tttccgaggt
     2461 agagcatgag catgaagaag acggagacct gcgtggtgaa cgccttcgtc gaggccaccc
     2521 ctatctcggg tcccgcgtgg gtgtatatga cgctgtggca ttcccttgaa agggtgctgc
     2581 ccagcacgtt gcatatggcg agggtgtgaa gccctttctt cttcacctct ttcatagccg
     2641 ctatggtgtc cgccgtctct cccgattgcg agacgagtat gacgagatcg ttgtctccga
     2701 ggatggggtc gcggtatcgg aattccgacg cgatgtcggt ttccaccggt attctcaggt
     2761 tcgcctcgaa gatatgcttg cctatgagac aggcgtggta cgaagtaccg caggcgacca
     2821 tccagatctt tcccactttc gtgatgtccg ggaggtcaag ctcttcgaag agtatctcgc
     2881 cctcttcctg ctgtatcctt ccgatcaggg tgtccgatat ggcccttggc tgctcgaaga
     2941 tctccttgag catgaaatgc ctgtagccgc ccttctcggc catcgcgccc gaccactgga
     3001 ctgaccgggc ttcccgctga acctcgtcgc cgtcgatgtt cgttatcttc agaacgccct
     3061 tctgtatgac cgcgatgtcg tcatcctcga ggaagatgaa cctgttggtc ctgttgatta
     3121 tcgccggggc gtcgctggct acgatgtact cgttgtcgcc cagacccacg aggagggggc
     3181 tttctttctt ggcggcgatc agggtgtcct ccccttgcct gatgatcccc accgcgtagg
     3241 atcccttgag catcctgaga gcggccctga cggcaccttc gaaacttccc gtcgtgccaa
     3301 ggaggctggc gatgagatgg ggtatgacct ccgtatccgt atccgatgtg aatgtgtgtc
     3361 cctcttcctt cagggcttcc ttcagttcga ggtagttctc tatgatcccg ttgtgaacga
     3421 cggtgatatc ggcggctgtg tgcgggtggg cattgcgctc cgagggaacg ccgtgggtcg
     3481 cccacctggt gtgggccaat cccatggacc cgtcaaagcc accatcgcgg agggcctcgg
     3541 ccaggtcgct caccttgccc tgtctgcggg caagcctcac ggccccgtcg ttccagatcg
     3601 ctatccccgc cgaatc
//
LOCUS       DAOQTR010000196         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29410_length_3600_cov_3.602821, whole genome shotgun sequence.
ACCESSION   DAOQTR010000196 DAOQTR010000000
VERSION     DAOQTR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29410_length_3600_cov_3.602821"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKN85_05730"
     CDS             complement(<1..451)
                     /locus_tag="PKN85_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNT43940.1"
                     /translation="MQGKQLIRTFFHEADVSHVFQLPGLHTLSLNAELFSQQSIKTIT
                     PRHEANLAFMADGYARASGKPAVIVVTPGPGLGNIVSGCMEAYSDDVPLLIVHIDTDR
                     KDMGKGILHELQSPEDIFRHFIKASFRADEPSILSSHLHDALKICNTP"
     gene            570..1124
                     /locus_tag="PKN85_05735"
     CDS             570..1124
                     /locus_tag="PKN85_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT43941.1"
                     /translation="MARSGEMSIGERIKVLRQAKNLTQEELATRAGLTKGFISQVERN
                     LTSLSVESLIGILDALDEKPSNFFNGAFEEKIVFKLEDRVDLEMEGVKGFQILIPAAQ
                     NRLLDPALLELRSGERTPEGEPHEGEEFGFVLSGSVELVLGGKAQKVKKGECFYYKAT
                     RKHYIINKLKATAVLLWVSSPPNF"
     gene            1286..1768
                     /locus_tag="PKN85_05740"
     CDS             1286..1768
                     /locus_tag="PKN85_05740"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNT43942.1"
                     /translation="MVTLIVRTPTRFFLVSGSSDGFSLLNAFDGALLASGVGDTNLVR
                     MSSILPPGCLEIKPRPAALPQGALVPVAYASLTSDVPGEVISAAVAIGIPEDPSLAGL
                     IMEYSARAEERVVLEQVRKMAEKGMEVRKRPIKEIMAISATFKVAAIGAVFAGVVLWD
                     "
     gene            1840..2676
                     /gene="speB"
                     /locus_tag="PKN85_05745"
     CDS             1840..2676
                     /gene="speB"
                     /locus_tag="PKN85_05745"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNT43943.1"
                     /translation="MEGVFHNRYISSRPDEGGPGIVILGCPLDVTSSFRGGSKFGPES
                     LRKASWTLETYSPYLGLDLDDMDLFDAGDLELPQGNLPGSLDRIEKSAARIIGPGRKP
                     LFLGGEHLVTYPLVKSMMGSFPLLQLVHFDAHCDLRDEYEGEKLSHATVMKRVRELGI
                     SGMHQIGIRSGTRQEFAELSPVHSPDGLAGSLERDIPIYISFDIDVFDPSLMSGVTTP
                     EPGGLMFGEVMDYLSVLKGMDIVGADLVELAPDYDTTFVSSVCAAKVAREIVMLLHSG
                     IK"
     gene            2712..>3600
                     /locus_tag="PKN85_05750"
     CDS             2712..>3600
                     /locus_tag="PKN85_05750"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNT43944.1"
                     /translation="MNNKDHGRIMIIGAGGVATVAAHKCAQVPEVFGEILIASRTLRK
                     CDAIRDDIRLRYGRDIGTAKVDADNVPELVALIRSFGPDVVLNLALPYQDLHIMDACL
                     ETGVHYIDTANYEPLDEAHFEYSWQWAYQDRFREKGIMALLGSGFDPGVTNVFAAYAR
                     KHLFDEIHYLDILDCNAGTHGHVFATNFNPEINIREVTQVVRHWRQGEWIETPAIIED
                     GSVHFTFDYPVAGNKESYLLYHEELESLARNLKGLKKARFWMTFSENYLTHLRVLRNV
                     GMTRIDEVDYEGNRVIPIKF"
BASE COUNT          813 a          940 c         1089 g          758 t
ORIGIN      
        1 gtggcgtgtt gcagatcttc agggcatcat gaaggtgaga cgacaggatc gacggttcgt
       61 cggccctgaa agaggcctta atgaagtgtc tgaatatgtc ctccggggat tgaagttcgt
      121 ggaggatgcc tttgcccatg tcttttctgt cagtatcgat gtggacgatg agaaggggca
      181 cgtcgtccga gtaggcttcc atgcaacccg acacgatatt gccgagaccg ggcccgggtg
      241 tgacgacgat gaccgccggt ttgcccgagg ccctcgcgta tccgtcggcc atgaaggcaa
      301 gattggcctc atggcgaggg gttattgttt tgatgctttg ttgagaaaaa agctcagcgt
      361 tcaggctaag tgtatgcaaa ccgggtaatt ggaagacatg ggaaacgtcc gcttcgtgaa
      421 aaaacgttct aattagttgt tttccttgca taatatgtcc agatggcata gtttagcata
      481 ctgaacctga aagttaaata aaaattacct tgacaaaaaa aacgcgaatc tagtttaata
      541 ctgtaaactt tttgtgcgta tatactgtta tggcaagatc gggagaaatg agcatagggg
      601 aaaggatcaa ggtgttgagg caggcgaaga accttaccca ggaggagttg gcgacgaggg
      661 cgggattgac gaagggtttt atctcgcaag tcgagcgaaa cctcacgtcc ctatccgttg
      721 agagtcttat cggcatactc gacgctctcg acgaaaagcc ttccaacttc ttcaacgggg
      781 cctttgagga gaagatcgtt ttcaagctcg aggacagggt cgatctggag atggaaggcg
      841 tgaaggggtt tcagatactc ataccggcgg cgcagaaccg tcttctggac ccggcgctcc
      901 tcgaactcag gtcgggtgaa aggaccccgg aaggggaacc ccacgagggc gaggagttcg
      961 gttttgtcct ttccgggagc gtcgaactgg tgctgggcgg gaaagcccag aaagtgaaga
     1021 aaggcgagtg cttctactat aaggcaacga ggaagcatta catcataaac aagctcaagg
     1081 cgacggcagt cctgttgtgg gtatcttccc ccccgaattt ctgaatgtcc cgtgcaagag
     1141 aacgggagat gcgagggagg ggaagaaaat ggcgagcaag caaagaccgg ggcggggttt
     1201 actctcgcct gttgcgttgt ggagcggctg aactctgccg gcaaatgaga tgccttggaa
     1261 ctgcgaggcc taatccattt gggaggtggt tacattgatt gtcaggacac ctacaagatt
     1321 ttttctagtc agcggctcgt ccgacggttt ttcgctgctc aacgcttttg acggagccct
     1381 tctggcttcg ggtgtgggcg atacgaatct ggtgagaatg agcagtatcc tgcctcccgg
     1441 ctgcttggag atcaagcccc gcccggcggc gctgccgcag ggagccctcg tgcccgttgc
     1501 ttatgcctcc cttacgagcg atgtcccggg agaggtcata tcggccgccg tggcgatagg
     1561 gatacccgag gacccatccc ttgccggcct tatcatggag tattcggcca gggctgaaga
     1621 gagagtcgtc ctggagcagg taaggaagat ggccgagaaa gggatggaag tccgaaagcg
     1681 gcccatcaag gaaataatgg ccatatcggc cacgtttaaa gtcgctgcca tcggcgccgt
     1741 gtttgccggt gtcgtcctgt gggactgatg gcggccctct gatggtcggc ttcacgccgg
     1801 cagtgttcgt gtaaccggag attcccagag gctattgcga tggaaggtgt ttttcacaac
     1861 aggtatatat cttcccgacc cgacgaggga ggaccgggca tcgtgatact tggctgtcct
     1921 ctcgacgtca cgtcctcttt tcggggcggg tcgaaattcg gccccgagag cctgaggaag
     1981 gcgtcatgga ccctggagac ctacagcccc tatctcggcc tcgacctcga cgacatggac
     2041 ctcttcgatg cgggggatct cgaacttccc cagggtaatc tccccgggtc cctcgaccgc
     2101 atcgaaaagt cggccgcaag gataataggg cccggccgca agcccctgtt cctcggcgga
     2161 gagcacctcg tcacatatcc tcttgtgaaa tcgatgatgg ggtcctttcc cctgcttcag
     2221 ctcgtccatt ttgacgccca ttgcgacctg agggacgaat acgagggaga gaagctctct
     2281 cacgccacgg tgatgaagcg cgtcagggaa ctcggcatat cggggatgca ccagataggc
     2341 atacggtcgg gtacacggca ggagttcgcg gagctgtcac ctgttcactc gcccgacggg
     2401 ctggccggaa gcctcgaaag ggatattccc atctatatca gcttcgacat cgacgttttc
     2461 gatccatctc tcatgtccgg tgtcacgacc ccggaaccgg gagggctgat gttcggggaa
     2521 gttatggatt acctcagcgt cctcaagggg atggatatcg tcggcgccga cctcgtggaa
     2581 ctggccccgg actacgatac caccttcgtt tcctccgtat gtgccgccaa ggtcgcgagg
     2641 gagatcgtca tgctcttgca ctccggaatc aaatagacca cgatcgattc gcttttcagg
     2701 agataaggac tatgaacaac aaggaccatg gaaggatcat gataatcggg gcgggagggg
     2761 ttgccacggt agccgcccac aaatgcgcgc aggtgcccga agttttcggg gagatcctca
     2821 tcgccagccg gaccctgcgg aagtgcgacg ccatcaggga cgacatcagg ctgagatacg
     2881 gcagggatat cgggacggcg aaggtggacg ccgacaacgt cccggagctt gtcgctctca
     2941 taaggtcttt cgggcccgac gtggtcctta acctggccct gccctaccag gaccttcata
     3001 tcatggatgc ctgtctcgag acaggggttc actacataga cacggccaac tacgagcccc
     3061 tcgacgaggc ccattttgaa tacagctggc agtgggcgta ccaggaccgg ttccgcgaaa
     3121 aggggatcat ggcccttctc ggttccgggt tcgaccccgg ggtgacgaac gttttcgcag
     3181 cgtacgcccg gaagcatctt ttcgacgaga tacactatct tgacatcctc gactgcaacg
     3241 cggggacgca cggccacgtc ttcgccacca acttcaaccc cgagataaac atccgcgagg
     3301 tcacgcaggt ggtgcgccac tggagacagg gggaatggat cgagacgccc gcgatcatag
     3361 aggacggcag cgtacatttc accttcgact accccgtggc gggaaacaag gaaagctatc
     3421 tcctgtacca cgaggaactg gaatccctcg ccaggaacct gaaggggctg aagaaggcca
     3481 ggttctggat gaccttttcc gagaactacc tgactcacct cagggtcctc cggaacgtgg
     3541 gaatgacgag gatcgacgag gtcgattacg aggggaacag ggtgatcccg ataaagttcc
//
LOCUS       DAOQTR010000197         3593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29479_length_3593_cov_4.078858, whole genome shotgun sequence.
ACCESSION   DAOQTR010000197 DAOQTR010000000
VERSION     DAOQTR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29479_length_3593_cov_4.078858"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKN85_05755"
     CDS             complement(<1..793)
                     /locus_tag="PKN85_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT43945.1"
                     /translation="MESAVRLDHNHELTVKDLPYIVTWREDRCKRCGRCTAVCPMHAI
                     EPSVMVRREISSEDASPSPKVTRKTVVVVRQTTDIERYCTGCGSCTLVCPNDAIEPVF
                     NPQHKFLFHKNPGGHPYRRGGRRNDPRESTLDHLKFTRISMLTDPALDAGRHEFRIRT
                     YLGRILPPEEMPFRVKEGKLELMSGNSRFVPPVREIYPVMIGSMSVGALSPTMWEGLA
                     MGVAYLNEVDRMPVVMASGEGGIPPRLLKSRFIKYFILQIASGYFG"
     gene            complement(754..1881)
                     /locus_tag="PKN85_05760"
     CDS             complement(754..1881)
                     /locus_tag="PKN85_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43946.1"
                     /translation="MCRLAAITSNEYLNPMECIGALETMHEGHDGSGMGLILKNLGGE
                     LAEFFDYPVLSGICSKDGLYTLEEYLFHAGFRLKHQWRPALKPVKGIKRRDHYFAKVY
                     DYPEEYEDRSQEEKEELLLQTRLTLRRMGEENGSITVFSFYPDIVTIKEVGDPLQLGE
                     FFGLTDSRLKAKIIFAQGRQNTNYDINLYACHPFFVQGFGSMTNGENTAFVPIREFLA
                     SRGFAGYSGYDSDSEVFTHILHYAVKKLGYPLHYYKDVITPLGEDDLARREDSEVLSL
                     MRVALRPLCIDGPNVIIGFTPDGSCFMVHDAKKLRPGVVGGVRGRVGLVSEECGLESM
                     VPERESAKDIFPMKYDMVIVSPGAQEVRVWNQLYGWTTIMN"
     gene            complement(2305..2973)
                     /locus_tag="PKN85_05765"
     CDS             complement(2305..2973)
                     /locus_tag="PKN85_05765"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNT43947.1"
                     /translation="MIVAERKTITELVEILRPHRNILVLGCGTCVTVCLSGGEREVSI
                     ISSALRIASRAQDLDFTISELTIERQCDNIFIEQAAEAIEKADAVLSTACGAGVQAIA
                     ERFPTKPVYAGLNTTFLGILEERGVWTEKCAACGSCVLHKYGGICPVTRCAKRVLNGP
                     CGGSREDRCEVRPDRSCAWQLIYRRLKDIGQLERLAEMEPPKNWNSSLSGGPRVLIRE
                     DHRV"
     gene            complement(2970..3431)
                     /locus_tag="PKN85_05770"
     CDS             complement(2970..3431)
                     /locus_tag="PKN85_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT43948.1"
                     /translation="MNANNSEPVIVAFCCHYCAYAAADLAGSGRLQYPPNIRIVRSPC
                     TGRLEVNFFLRALEAGADGVLVAGCEEGSCHFKEGNYLAKGRVNTARQLLKEAGLEPD
                     RLRMVNVSAASSQKFAECVREMVETVKKLGPSPVKRTIRRNTNQEQTGAIQ"
BASE COUNT          696 a         1079 c         1039 g          779 t
ORIGIN      
        1 atccgaagta gcccgaggct atctggagga tgaagtactt gatgaatctc gacttgagga
       61 gcctcggcgg gatgccgccc tcaccggacg ccatgacgac gggcatgcgg tcgacctcgt
      121 taaggtacgc gacccccatc gccagccctt cccacatagt tggcgacagg gcgccgacgg
      181 acatgctgcc gatcatgacg gggtaaatct cgcgcacggg cgggacgaac cgggaattcc
      241 cgctcataag ctccagcttt ccttccttta ccctgaaggg catctcttcg ggaggcagaa
      301 tgcgcccgag ataggtgcgt atcctgaact cgtggcggcc ggcatcgagc gccgggtccg
      361 tgagcatcga tatacgggtg aacttcaggt ggtccagcgt cgattcccgg gggtcgttcc
      421 gcctgccgcc cctgcggtac gggtgtcctc ccgggttctt gtggaagagg aacttgtgct
      481 gggggttgaa aacgggttct atggcatcgt tggggcacac aagggtgcag ctgccgcaac
      541 ccgtgcagta gcgctctatg tccgtggtct gcctgaccac cacaaccgtt ttccgcgtga
      601 ccttcggaga aggagatgcg tcttcggagc ttatctcgcg cctcaccatc accgagggct
      661 cgatggcgtg catgggacag acggcggtgc accggccgca tctcttgcac ctgtcctcgc
      721 gccacgtgac gatataggga aggtccttca ctgtcagttc atgattgtgg tccagccgta
      781 cagctgattc catacccgca cctcctgcgc tcccggtgaa acgatgacca tgtcatattt
      841 catggggaag atatctttgg cactctctct ttcgggaacc atgctttcga gcccgcactc
      901 ctccgacact agtcctaccc tgcccctaac gccgcccaca accccggggc ggagcttctt
      961 cgcgtcgtgg accatgaaac aggagccgtc gggagtgaac ccgatgatga cgttgggccc
     1021 gtcgatgcac aggggccgaa gggccacccg catgagcgag agcacctcgc tgtcctcacg
     1081 ccttgcgagg tcgtcctcgc cgaggggagt gataacgtcc ttgtaatagt gcaggggata
     1141 gccgagcttc ttcaccgcgt agtggaggat gtgcgtgaag acctcgctgt cgctgtcgta
     1201 tcccgaatac cccgcgaacc ccctgctggc gagaaactcc ctgatgggca cgaaggccgt
     1261 gttctcgccg ttcgtcatgg aaccgaaacc ctgaacgaag aaggggtggc aggcatagag
     1321 attaatgtcg taattcgtgt tctgccgtcc ctgggcaaag atgatcttcg ccttcagacg
     1381 ggaatcggtg aggccgaaga attcccccag ctgcagggga tctcccactt ccttgatggt
     1441 gacgatgtcc gggtagaaag aaaagaccgt tatggaaccg ttctcctccc ccatcctgcg
     1501 aagggtaagc cgcgtctgga gaagaagctc ctccttctcc tcctggctcc ggtcctcgta
     1561 ctcctcgggg tagtcgtaga ccttggcgaa gtagtggtcc ctgcgcttta tgccctttac
     1621 cggcttcagc gcgggccgcc actggtgttt cagccggaac ccggcatgga agagatactc
     1681 ctcgagggtg tagagcccgt ctttggagca gatccccgaa agcaccgggt agtcgaagaa
     1741 ctctgcaagc tctccaccca ggttcttgag gataagcccc atccccgagc cgtcgtggcc
     1801 ctcatgcatc gtctccaggg ctccgatgca ttccatgggg tttaagtatt cattcgatgt
     1861 gatcgcagcc aggcgacaca tgacagcctc ctttccggta ttctctcaaa gcgtcttccc
     1921 aggatctacc tcaggcggtg ggtttagtac cgggacatgt cccgcggaga gaaactcaac
     1981 acgcgatcct atacactcaa aacgcttgag catcggggat aattgtatcg ctgaagggga
     2041 ggatacgtca acggcttacg cgccggggaa ggcccatgaa acgaataatg accaatgatc
     2101 aaattccaat gaccaatgaa aaaggcaata accaattgaa caaagaaaag ggcggaacgg
     2161 accgcccgta tcattttggt ttattgatca ttgtctgttt atctggttat tggaatttgg
     2221 tcattggtca ttatcctctc ccaacgggtg ctctggagcg tcagcgtggc cgtggccgcg
     2281 aatctgattt gaacaggcac ggctctatac cctgtggtct tcccgtatca ggactctcgg
     2341 gccaccggag aggctggaat tccagttctt gggcggttcc atctccgcga gcctttcgag
     2401 ctggcctatg tccttgagcc tgcggtagat gagctgccac gcgcaggacc tgtcgggacg
     2461 cacctcgcac ctgtcctccc gggagcctcc gcagggcccg ttgagcacac gtttcgcgca
     2521 gcgggtgacg gggcatatgc cgccgtactt gtgcaggacg caggaaccgc aggcggcgca
     2581 tttctccgtc cacaccccgc gttcttccag gatgccgagg aaggtcgtgt tcagtccggc
     2641 atacacgggc tttgtcggga aacgttcggc gatggcctgc accccggcgc cgcacgccgt
     2701 cgagagcacg gcatcggcct tctcgatggc ctccgcggcc tgctcgatga agatgttgtc
     2761 gcactgccgc tcgatggtga gttcgctgat ggtgaagtcg aggtcctggg cacgggaggc
     2821 aatacgcagc gccgacgata tgatgctgac ctcgcgttca ccgccggaga ggcacacggt
     2881 aacgcaggta ccgcagccaa gcaccagtat gttcctgtgg ggccttaaga tctcgacgag
     2941 ttcagtgatg gtctttcttt cggcgactat cattgtattg ctcctgtctg ttcctggttt
     3001 gtatttcttc taatcgtcct cttcacaggg gaaggaccaa gctttttgac ggtttccacc
     3061 atctcgcgga cacattcggc gaacttctgg gaactcgccg cgctcacatt gaccatgcgc
     3121 aggcggtcgg gctcgaggcc agcctctttg agcagttgcc tcgcggtatt cacccggccc
     3181 ttcgccaggt aattgccttc cttgaagtga cagctccctt cctcacatcc ggcgacgagc
     3241 acgccgtcgg caccggcctc caaggccctg aggaaaaaat tgacctccag cctgcccgtg
     3301 cacggcgagc ggactatgcg gatattgggc ggatactgca ggcgcccgga accggcaagg
     3361 tctgccgctg cgtaggcgca atagtggcag caaaaggcca cgatcacggg ttcggaatta
     3421 tttgcgttca taaccatttt ccctctctcg ctgcctcttc ggcagtgctc ctgtcctcac
     3481 actatcctgc cgccagaagt gcctccagca tgctctcaaa ctgggcggac tcgaaatttc
     3541 tgagctgtat ggcctcggcc ggacattccg aggcgcatat gccgcagccc atg
//
LOCUS       DAOQTR010000198         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29578_length_3585_cov_3.389518, whole genome shotgun sequence.
ACCESSION   DAOQTR010000198 DAOQTR010000000
VERSION     DAOQTR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29578_length_3585_cov_3.389518"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PKN85_05775"
     CDS             complement(<1..1113)
                     /locus_tag="PKN85_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT43949.1"
                     /translation="MIDKTLQDLKALVAVASAEREPDVIIEGGEVFDVFTGEVFPGDL
                     WICGNWIAYVGEKRTKTGKDTQVVDARGLVAVPGYLDAHGHADIFYNPATFSDVAVTK
                     GTTTVFSDSHDLMSCLGPKGFAEVLRKARYFNIKYLWGVPATYPPYPEVEGGEMFSIY
                     DIWDLFSRHPECASVSEVSSYLRIIRNEDEILERMLLARSLGRNVEGHTLGASYDRLN
                     ALVAAGITSCHESIRPADLKNRIRLGLYTMVRHSSIRTDLEELGPAINTLPNDSIILV
                     SDGIFADDLMFRGYMDHIVGEAIRFGVEPQNAIRMATINPARYFRVDSEVGSLTPGRI
                     ADVLLLEGLDRPVPVKVFERGTLAAEGGTLTRRPSPE"
     gene            complement(1411..2661)
                     /locus_tag="PKN85_05780"
     CDS             complement(1411..2661)
                     /locus_tag="PKN85_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT43950.1"
                     /translation="MERHRRYLFLILAAQYLVVYFHRVSPAVVAQDLAASFGISATAL
                     GVLASAYFYPYAAMQVPVGILSDSWGPKKTIVLCSLIAALGAIAFGLAPCFSVAVGAR
                     SLVGLGLAAVFVSSMKIFGLWFRGAELARVAGALMAVGGIGWYSATTPLALLSEVFGW
                     RSAVVIVGVLSLGVIFLIWRFVEDSPEKKGLPPVVGAGEGVVTGRRNVLKDLKVILRN
                     RYFWTIAIWFIMRGGALFGFFGLWAGPYLTDVYGFSKATAGNILSMIAFAMIFVSPVI
                     GHLSDRTLKSRKKILVWSSALNVICFACAVLFFDGLGLVALYVLFFFMGITISSVGTV
                     AIVTTKELFPDEIAGTSMGTINLFPFIGAIIFQPLMGYVLDRAGMTGGSYPPSAYKQM
                     LWIYLITSILALFSIMKCKETFKG"
     gene            2777..>3585
                     /locus_tag="PKN85_05785"
     CDS             2777..>3585
                     /locus_tag="PKN85_05785"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT43951.1"
                     /translation="MRQGTLKRLLDVVKGRTRPDTVIVNGKIVNVFTNSIDEGSSVIV
                     KDGFIASVEENGKATSYHPKRVIDAHGAYLCPGFIDSHTHIDSLYPFYALVPYAIRGG
                     TTTVVTETSAVACACGMEGVNAFTESTRGYPLRCFFLAPPLTPPFPEMEGCKGLTLGE
                     FKRFLRRQDVPAIGEAYWTRIVEGDQRVLEQAVSAINANKKLDGHAAGARAKRLVQYV
                     LTGITSCHESVTVDEVIEKLRLGLTIMIRQGFVRKELPELHRLKDMDIDTR"
BASE COUNT          800 a         1085 c         1043 g          657 t
ORIGIN      
        1 ctccggtgac ggccgacgcg tgagcgtccc tccctccgcg gcaagggtcc cgcgctcgaa
       61 gaccttgacg gggacaggtc tgtcgaggcc ctcgagaagg agcacgtccg ctatcctgcc
      121 cggcgtcagg ctgcccacct ccgaatccac cctgaagtac ctggccggat tgatggtggc
      181 cattcggatg gcgttctgcg gctcgacccc gaaacggatg gcctcgccca cgatatggtc
      241 catgtatccc ctgaacatca gatcgtcggc gaagatgccg tcggacacga ggatgatgga
      301 atcgttggga agggtgttta tggcggggcc caactcctcg aggtcggtcc tgatggagct
      361 gtggcggacc atcgtgtaaa gccccagccg tatccggttc ttcaggtccg cggggcggat
      421 cgattcgtgg caggatgtga tgcccgccgc gacaagggcg ttcagccggt cataggaggc
      481 gccaagcgta tgaccctcga cgttcctccc cagggaccgg gcaaggagca tcctttccag
      541 tatctcgtcc tcgttcctta ttatcctcag gtatgatgag acctcgctca cggaagcgca
      601 ctcggggtgg cgcgaaaaaa ggtcccagat atcgtagatg gagaacatct cgccgccctc
      661 cacctccgga tagggtggat aggtcgccgg aacgccccag agatatttga tgttgaaata
      721 ccgcgccttt ctgaggacct cggcgaaccc tttgggcccg agacagctca tcaggtcatg
      781 actgtcggaa aagacggtcg ttgtcccctt cgtcacggca acgtcggaaa acgtcgccgg
      841 gttgtagaat atgtccgcgt gtccgtgggc gtccaggtag ccgggtacgg cgacaagacc
      901 ccgcgcgtcg acgacctggg tgtccttccc tgtcttcgtt ctcttttccc ccacataggc
      961 gatccagttg ccgcagatcc acaggtcgcc gggaaagacc tctcccgtga agacatcgaa
     1021 cacctcgccg ccttctatga tgacatccgg ctcccgctcg gcgctggcaa cggccacgag
     1081 ggccttcagg tcctggagtg tcttgtcgat cattatggtt ctgtcctgcc ggtcatcttt
     1141 cccttggagt acgtttcaat gtagcccaaa acccccttcg atggcaagag attcgaaaat
     1201 ggttcaaggg ttcaagggtt caaggattca agggttcaag ggaaagaccg cgaggggggt
     1261 cggctgccaa gggaattcgt acaccggccg aaggcgccgg tgcctttttt gtcaagacac
     1321 ttcgcgacct gcgcgacatt ctgttcctgt tcttgaccct ggaactcttg aacttttgaa
     1381 ctcttgaacc ttcttcatcc ttgaacgcct ttagcctttg aaggtttcct tgcatttcat
     1441 aatgctgaaa agggccagaa tgctggtgat gaggtatatc cagagcatct gcttgtacgc
     1501 ggacggcggg taggagccgc ccgtcatgcc cgccctgtcg agaacgtagc ccataagcgg
     1561 ctggaagatg atggccccga tgaaggggaa caggttgatg gtgcccatgg acgtgcctgc
     1621 tatctcatcg gggaaaagct cttttgtggt gacgatggcg acggtcccga cggagctgat
     1681 cgtgatcccc atgaagaaga agagcacata gagcgcgacc agcccgagac cgtcgaagaa
     1741 aagaacggca caggcaaaac agataacatt gagggccgag gaccatacga ggatcttctt
     1801 cctgctcttc aacgtcctgt ccgaaaggtg gccgatgacg gggctcacga atatcatcgc
     1861 gaaggctatc atggagagga tgttgccggc ggtagccttg ctgaacccgt acacgtccgt
     1921 gagatacggc cccgcccaga gcccgaagaa cccgaaaagg gcccctcccc tcatgatgaa
     1981 ccagatagct atggtccaga aatacctgtt cctgaggatg accttcaggt ccttgagcac
     2041 gttgcgcctg cccgtcacaa cgccctcgcc cgcccccacg acgggaggca gacccttctt
     2101 ctccggtgag tcctcgacaa accgccatat gaggaatatg accccaaggg agaggacacc
     2161 cacgatgacg accgccgacc tccatccgaa aacctccgac agcagggcga gaggtgttgt
     2221 cgcggagtac cacccgatac cccccacggc catgagggca ccagcgaccc tggccagctc
     2281 ggccccccgg aaccagaggc cgaagatctt catcgacgac acgaagacgg ccgcaagccc
     2341 cagccccaca agggagcggg ccccgacggc aacgctgaaa cagggggcaa ggccgaacgc
     2401 gatagcaccc agcgcggcta tcaagctgca aagaacgatc gtcttcttcg gcccccacga
     2461 atccgagaga atgccgacag ggacctgcat tgccgcgtag gggtagaagt aggccgatgc
     2521 gaggacgccg agggccgttg ccgatatccc gaagctggcg gctaggtcct gggccaccac
     2581 ggcgggtgat acacggtgga aatagaccac gaggtactgc gcggcaagaa taagaaagag
     2641 gtaccgcctg tgacgctcca cgccgaactc ccccaaatga cgaaagaagc tgaaatacct
     2701 acaacaatac tttgcaggac aggccttttt caagtaaaat agagaaattc ggctgagacg
     2761 ggcaaaaata gggacaatga gacagggcac tctgaaaagg cttctcgatg tcgtcaaggg
     2821 caggacacgg cccgacacgg tcatcgtcaa cgggaagatc gtcaacgtct tcaccaacag
     2881 catcgatgaa ggctcgtccg tcatcgttaa ggacggtttc atcgcctccg tcgaggagaa
     2941 tggaaaggct acctcttacc atccgaagag ggtaatagac gcgcacgggg cctacctgtg
     3001 tcccgggttc atcgactccc acacccacat cgacagcctc tatccattct atgccctcgt
     3061 cccctacgct atccggggcg gcacgacaac ggtcgtaact gaaacgagcg ccgtcgcctg
     3121 tgcctgcggc atggaagggg tcaacgcctt cacggaaagc acgcggggtt acccgttgcg
     3181 gtgtttcttc cttgcccctc cgctcacgcc cccctttccg gaaatggagg gctgcaaagg
     3241 gctcaccctc ggggagttca aacgcttcct caggcggcag gacgtgcccg ccataggcga
     3301 ggcctactgg acacgcatcg tcgaaggaga ccagcgggtc ctcgagcagg cggtgagcgc
     3361 cataaacgcc aacaagaagc tcgacggcca cgccgccggg gcccgcgcga aaaggctcgt
     3421 tcaatacgtc ctcacgggca tcacatcctg ccacgagtcg gtgacggtcg acgaggtcat
     3481 cgaaaagctc cgcctcggcc tcaccatcat gatacggcag ggcttcgtca ggaaggaact
     3541 ccccgagctc catcgactga aggacatgga tatcgatacg agaag
//
LOCUS       DAOQTR010000199         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29617_length_3582_cov_4.224553, whole genome shotgun sequence.
ACCESSION   DAOQTR010000199 DAOQTR010000000
VERSION     DAOQTR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29617_length_3582_cov_4.224553"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..476)
                     /locus_tag="PKN85_05790"
     CDS             complement(216..476)
                     /locus_tag="PKN85_05790"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNT43952.1"
                     /translation="MRFLILIACIVIVVAIFSTQNSQPVSVSFFVWNFQASLAIVVFL
                     SVICGLVLGVLASFFLGLTRKRKAAAGKGPGTTPAEPDGTGS"
     gene            complement(517..1728)
                     /locus_tag="PKN85_05795"
     CDS             complement(517..1728)
                     /locus_tag="PKN85_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43953.1"
                     /translation="MSDFYQTGVVPTLHRLGKDNLEEIESRLRRYAQDRPIALVLPSL
                     YSELSGAALGGIVEELKKVQYIKEIVVTLGPASDSEFQNARRFFSALPQKVCVIWNNG
                     KRMKKLYRAIEAEDLPTGLEGKGRSVWMAYGYVLARREFNAIALHDCDILTYSRDMLA
                     RLCYPVTNPSLDYDFCKGFYTRVTDKMHGRVTRLLVTPLIRALVKIIGFHPLLVFFDS
                     FRYILAGEFSMDVNLARINKIPGDWGLEVGTLAEVYKNTSINRVCQVDIAENYEHKHQ
                     ELSPEDASKGLNKMCVDICKSIFRTLTSEGIVFSDGFFKTLFATYARTAQDHLKRYED
                     DASINGLRFDRHEESLSVETFTKGIKTAAEVIMKDPLGVPLISSWDRVTAALPDILDR
                     IREAVEEDNRS"
     gene            1912..2739
                     /locus_tag="PKN85_05800"
     CDS             1912..2739
                     /locus_tag="PKN85_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNT43954.1"
                     /translation="MPVRRAVVFTDLDGTLLDARTYSFDPALPALGMLRERHVPVVIC
                     SSKTRAEIEHYRAKLRNADPFISENGGAVFVPHGYFRADAVSPARPEGGDEGPHLVLR
                     LGARYQDLRRALRELRGEGFDVRGFGDMTAEEIAAATGLPLNEAVMAAKREFDEPFLF
                     GGDAVMGDKLRASIERKGFRVTQGLFHHILGESDKGKAVSILTGMFRREYGEVTTVAL
                     GDSPNDLPMLQAVDRPIIVEKHGGGYDRVLVAEDFDRARGAGPDGWNSAIMRLLKDL"
     gene            complement(2763..>3582)
                     /locus_tag="PKN85_05805"
     CDS             complement(2763..>3582)
                     /locus_tag="PKN85_05805"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT43955.1"
                     /translation="VVLGSVAAVSISGILIGGIFPGLIMAFLYAAYIIGRTFLQPSMA
                     PDYEVPRSDPVSIVKELLVYVMPLTSVVALVLGPIYMGIATPTEAASLGCVGALTLSI
                     VYGKLTFSIFSEALAATLRTSSMIFLIIAGSVGYSQIISFSGAGAGLISAVVSLNLPD
                     VLLITILLSMLLLLGCFMDQIAMMMVTVPLFMPIVYQANIDPLWFGIMMLIALDIGFT
                     SPPFGLLLFVMKGITSEDITMLDIYKATIPFIACNLFTLTLILAFPRIVMVFLK"
BASE COUNT          765 a          980 c         1112 g          725 t
ORIGIN      
        1 atgtgctcct tcaggaaata ctggtacgtg tgatggaata tggaatcatc gcttaccctg
       61 gcgatcacct cccgcaattc cttgaggtcc gccgccttct cgcccgttga ctttattatg
      121 cttatgcact gcctgaagtc aaaagggcgc tccaattctt cgtgtccttc catacgtttc
      181 acctcaccga tgagcgcggt cccggagacc cgcgatcacg acccggtccc gtccggttcc
      241 gccggggttg ttccgggccc cttcccggcc gccgcttttc gtttcctggt cagccccagg
      301 aagaatgacg ccagcacacc gaggaccagg ccgcatatga ccgagagaaa gaccacgatg
      361 gccagagagg cctggaagtt ccagacgaag aaggagacgg agaccggctg cgagttctgg
      421 gtggagaaaa tggccacgac aatgacgatg caggcgataa gtatgagaaa acgcatcttg
      481 cctccctcgg ccccttcccg gccggcctcg cgttcttcag gaccggttat cctcctcgac
      541 cgcctcccgt atcctgtcga gtatgtcggg caatgccgcc gtcaccctgt cccagctcga
      601 gatgagggga actcccaggg ggtctttcat gatcacctca gcggcagtct tgatgccttt
      661 cgtgaaggtt tccacggaga gactttcctc gtgacggtcg aaacgcaggc cgttgatgga
      721 ggcgtcatcc tcgtacctct tcaggtggtc ctgtgcggtg cgtgcatagg tggcaaagag
      781 tgtcttgaaa aaaccgtcgg aaaagactat tccctcggag gtcagggtcc ggaatatgga
      841 cttgcagata tccacgcaca tcttgttgag gcctttcgag gcgtcctcgg gggaaagctc
      901 ctggtgcttg tgttcgtagt tctcggcgat gtcgacctgg cagacgcggt ttatggaggt
      961 atttttgtag acctccgcga gtgttcccac ctcaaggccc cagtcgccgg gaatcttgtt
     1021 gatgcgggcg aggttcacgt ccatggagaa ctcgcccgcc aggatatagc ggaaactgtc
     1081 gaagaagacc aggagagggt gaaatccgat gatcttgaca agggcgcgta tgaggggggt
     1141 cacgaggagc cgggtgaccc tcccgtgcat cttgtccgtg actctcgtat agaagccctt
     1201 gcagaaatcg tagtcgaggc tggggttcgt gacgggatag cagagcctcg cgagcatgtc
     1261 gcgcgaatag gtgaggatgt cgcaatcgtg cagggcaatg gcgttgaact ccctccgcgc
     1321 caaaacgtag ccgtaagcca tccacacgga gcggcccttg ccttcgaggc ccgtgggaag
     1381 gtcttccgcc tcgatggccc ggtacagttt cttcatcctc tttccattgt tccatatgac
     1441 gcacaccttc tgggggaggg cggagaagaa acgccgggcg ttctgaaact cgctgtccga
     1501 cgcgggaccc agggtcacga cgatctcctt gatatactga acctttttca gttcttcaac
     1561 gatcccaccg agtgcagcgc cggaaagctc gctgtacagg gagggaagga cgagtgcgat
     1621 gggcctgtct tgagcgtatc gcctgagcct cgattcgatc tcctcgagat tgtcctttcc
     1681 cagtctgtgc agcgtgggga caacgcctgt ctggtaaaag tcggacatgc tcacctcctg
     1741 gaatgtagtc tagcactcta tccgagaagc ggtggaaaat ctgtaaacta ttttacactc
     1801 tcctgaaacc gctatatggt agcatatcat aagtatgata atacgtcaca aaaaagggtc
     1861 gcgggctcac atgtgccgtt cgccttcggg cgggggtgcc ggaaaggctg tgtgccggtg
     1921 agaagagccg tcgtgttcac cgacctcgat ggaaccctcc tcgacgcgcg gacctattcc
     1981 tttgatccgg ccctgcccgc cctcggcatg ctcagggaga gacatgtccc cgtagtgatc
     2041 tgttccagca agacacgggc cgagatcgag cattatcgcg caaaactgcg taacgccgac
     2101 cctttcatct cggagaacgg aggcgcggtc ttcgtgccac acgggtattt cagggccgac
     2161 gcggtgtcgc cggcgaggcc cgaaggaggc gacgaggggc cgcatcttgt cctccggctt
     2221 ggcgccaggt atcaggacct gcgcagggcc ctgagagagc ttcgcggcga ggggtttgac
     2281 gttcgcggat tcggagatat gaccgccgaa gagatcgccg ccgcgaccgg cctgcccctc
     2341 aacgaagcgg tcatggcggc aaagcgggaa ttcgacgaac ctttcctctt cgggggagac
     2401 gcggtgatgg gggataagct gcgggcctcc atagaaagga agggcttcag ggtcacgcag
     2461 gggcttttcc atcatatcct gggcgaaagt gacaagggta aggctgtttc catcctcacc
     2521 ggaatgttcc gcagagaata tggcgaggtg acgacggtgg ccctggggga cagccccaat
     2581 gacctgccga tgctccaggc tgtcgaccgc ccgatcatcg tcgaaaaaca cggcgggggc
     2641 tacgaccggg tgcttgtagc ggaggatttc gaccgggccc ggggagcggg accggatggt
     2701 tggaacagtg cgataatgag actcctcaag gacctgtgaa tcctgtccca cggtccttca
     2761 cgtcatttca gaaaaaccat cactatgcgc gggaacgcga ggatgagagt gagggtgaag
     2821 agattgcagg caatgaaggg gattgtcgcc ttgtaaatgt ccagcatggt gatatcttcc
     2881 gaggttatgc ctttcatcac gaaaaggagg aggccgaagg gcggcgaggt gaagccgatg
     2941 tcgagggcga tgagcatcat gatcccgaac cagagcgggt ctatgttcgc ctggtagacg
     3001 atgggcatga agagggggac cgttaccatc atcatggcta tctggtccat gaaacagccg
     3061 agcagaagca gcatcgaaag caggatcgtg atgagaagga cgtcgggcag gttgagggag
     3121 acgacagcgg agatgaggcc cgctccggcg ccgctgaagg aaatgatctg gctgtacccg
     3181 acggaaccgg ctattatgag gaagatcatc gaactcgttc tcagcgtggc cgccagggct
     3241 tccgagaata tcgagaatgt gagctttccg tacacgatcg aaagggtcag ggctcccacg
     3301 catcccaggg aggcggcctc ggtgggtgtg gctatcccca tgtatatcgg gcccaggacg
     3361 agggctacga cgcttgtcaa aggcatgacg taaacgagaa gctccttcac gatgctcacg
     3421 gggtccgatc tcgggacctc gtaatccggg gccatcgagg gctggaggaa agtcctccct
     3481 atgatataag cggcgtagag aaaggccatg atgagtcccg ggaatatgcc ccctatcagt
     3541 atccccgata tggataccgc ggccacggag ccgaggacga cg
//
LOCUS       DAOQTR010000200         3574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29706_length_3574_cov_3.159136, whole genome shotgun sequence.
ACCESSION   DAOQTR010000200 DAOQTR010000000
VERSION     DAOQTR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29706_length_3574_cov_3.159136"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..324
                     /locus_tag="PKN85_05810"
     CDS             55..324
                     /locus_tag="PKN85_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNT43956.1"
                     /translation="MDIAVYLICLVSGAVLGLLHFGSLWVMVSRLGRVRRPVVLVFGR
                     FLVMMAVTLVGLYLIMGGRWERLIAALAGFMLVKVIVARRVRGGR"
     gene            361..1197
                     /locus_tag="PKN85_05815"
     CDS             361..1197
                     /locus_tag="PKN85_05815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNT43957.1"
                     /translation="MSLKEISPDAVILWKWGFVHVNLTMVFTWAIMALLVLACWIVTR
                     DLSTGTAVSRWQNLLEILVSAMRDHIRDVTRSEPGPYLSFIGTLFLFIAVSNFLAVVP
                     GYVPPTGSLSTTAALAICVFVAVPLYGIMDQGVSGYLRHYIRPTVFMLPFHVIGELSR
                     TLALAVRLFGNIMSGTKIVAILLAVTPFIFPIVIQALGLLTGMIQAYIFSILAMVYIA
                     SATRARGDGGTKRTKETARTAERTGKNNPHGPSSTGSGLAHQGMEGREGQERRRSWTA
                     SL"
     gene            1179..1448
                     /locus_tag="PKN85_05820"
     CDS             1179..1448
                     /locus_tag="PKN85_05820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNT43958.1"
                     /translation="MDSITLIGMASVITSGITIAIGSVGPALGEGRAIAQALSAIAQQ
                     PDESGTITRTLFVGLAMIESTAIYCFVISMILIFANPFWDHVIAK"
     gene            1458..2261
                     /locus_tag="PKN85_05825"
     CDS             1458..2261
                     /locus_tag="PKN85_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43959.1"
                     /translation="MLIDWFTVVAQIVNFLVLVALLKYFLYGRVINAMDKREEKIVSR
                     LTDAEEKERRAEEEARSYQEKSREVEEKRGELLARVEEEADGKRKELIRKAREEVAGL
                     EKGWREALMQEQDIFLEELRGHVAREVCVIARRIMKDLASAELETQVVNTFVMRIRDL
                     DKEERNALPSGTGEMNGRLAIVSAFDIGKDDRRKISEALRNTLKKSVDLEFRTSSDMV
                     LGIELRSRDHKLAWTVDDYLGSLEAGIRDVFEEEAREGIRDDEEGPQKT"
     gene            2258..>3574
                     /locus_tag="PKN85_05830"
     CDS             2258..>3574
                     /locus_tag="PKN85_05830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNT43960.1"
                     /translation="MTESVSQKETVLPVLERTFSAFDRAMDELRPRLTVEEIGVLTFV
                     SKGIARVKGLPTVRSGELLLFSGNIQGIAFNTDLEEIGVVLLDQGATLGAGSLVRRTG
                     RVLDVPVGEALIGRVIDPQGRVLDGQGPLRSTRRRPIEKAAPAILDRLPVTVPLETGL
                     KVIDALIPIGRGQRELILGDRQTGKTAIAVDAMINQGGKGVVCIYCAIGKRGSAVAKV
                     VADLNQYGAMGHSIVVVATEEHPPGLQFTAPYAAMTIAEFLMGRGRDVLVVFDDLTRH
                     ARAYRELSLLLRRPPGREAFPGDIFYVHSRLLERSTHLKEELRGGSITALPVVETEAQ
                     NISAYIPTNLISITDGQIYLSPDLFQKGVLPAVDVGRSVSRVGGKTQFHAYRVVAGDL
                     RLAYSQFVELESFARFGTRLDEETRKTLERGRRVREVLKQPQYSPMK"
BASE COUNT          713 a          948 c         1154 g          759 t
ORIGIN      
        1 gcggtcaatt ttcttctcga ccgctggccg caaaaggaag agaggtagat caccatggat
       61 attgccgtct acttaatctg cctggtatcg ggagcggtcc tgggattact ccatttcgga
      121 tcgctctggg tcatggtgtc ccgtcttggc agggtcaggc gaccggttgt gctggtcttc
      181 ggccgtttcc tcgtcatgat ggcggtgacg ctggtcggtc tctatcttat catggggggt
      241 cgatgggaaa ggctcattgc cgctcttgcc ggtttcatgc tcgtcaaggt tattgtcgcc
      301 aggcgtgtgc ggggggggcg ctaggggcac cccgggcaaa gtgaggtttc cttggatccc
      361 atgagcttga aagagatcag ccccgatgcc gtcatcctct ggaaatgggg gtttgtccac
      421 gtgaatctga ccatggtctt cacgtgggcg atcatggcat tgcttgtgct tgcctgctgg
      481 atcgtcacgc gggatctatc gacgggaacg gccgtttcca ggtggcagaa tcttctggag
      541 atattggtga gcgcgatgag ggatcacata cgtgatgtca cgaggagcga gccggggccc
      601 tacttgtcct ttatcgggac gttgttccta ttcatcgcgg tctccaattt tcttgcagtg
      661 gtgccgggat atgttcctcc aaccgggtcg ctttccacca cggcggccct cgccatatgc
      721 gtctttgttg ccgtgcctct gtacgggatc atggaccagg gagtgtccgg gtatctcagg
      781 cactatatca gaccgacggt gttcatgctc ccttttcacg tgatcggtga actgtccagg
      841 acgttggccc tggctgtcag gcttttcggg aatatcatga gcgggacgaa gatcgtcgcc
      901 atcctgctgg ccgttacacc gttcattttt cctatcgtca tccaggccct gggtctcctg
      961 acaggaatga tccaggccta catcttttcg atacttgcga tggtgtacat cgcgtccgcg
     1021 acgcgggccc gaggcgatgg ggggacaaag agaacgaagg aaacggcacg aacagcggag
     1081 cgcaccggta aaaacaaccc tcatggcccc tcttcgacgg gctcgggact cgcgcatcag
     1141 gggatggagg gccgcgaggg acaagaaagg agaagatcat ggacagcatc actctgatcg
     1201 gtatggcttc agtcattaca tcggggatca cgatcgccat cggctcggtg ggtccggctc
     1261 tcggggaagg gagggcgata gcccaggccc tgagcgcgat cgcccagcaa ccggatgaat
     1321 cggggacgat cacgcgcacc cttttcgtgg gtcttgccat gatcgaatcg acggcgatct
     1381 actgtttcgt catctccatg atccttatct tcgccaatcc cttctgggac catgtgatcg
     1441 caaagtagga gggttccgtg ctcatcgatt ggttcacggt cgttgcccag atcgtaaatt
     1501 tcctggtcct cgtggccttg ctgaaatatt tcctttacgg cagggtcata aacgcgatgg
     1561 acaaacggga ggaaaagatc gtctcccgcc tcaccgacgc agaggaaaag gaaagacggg
     1621 cggaggagga agcccgctcc tatcaggaga agagccgcga ggtcgaggag aagcgcgggg
     1681 agctgctggc ccgggttgaa gaagaggctg acggaaagag aaaagagctg atccggaagg
     1741 cccgcgaaga ggtggccggg cttgaaaagg gatggcgcga agccctgatg caggaacagg
     1801 atatattcct cgaggaattg cgggggcatg tggcccggga ggtctgcgtc atagcccggc
     1861 ggatcatgaa agacctcgcc agcgccgagc tcgagaccca ggtcgtgaat acctttgtca
     1921 tgcgcatcag ggatctcgat aaggaggagc ggaacgcgct tccgtccggc acaggggaga
     1981 tgaacggtcg tcttgccatc gtgagcgcct ttgatatcgg gaaggatgac cggcgcaaga
     2041 tctcggaagc tctgaggaat acgcttaaaa agagcgtcga tcttgaattc cggacatcgt
     2101 cggatatggt cctcgggatc gaattgagaa gccgggacca caagcttgcc tggaccgtcg
     2161 atgattacct gggctcgctg gaggccggaa tcagggacgt cttcgaggaa gaggcgcgcg
     2221 agggcataag ggatgacgag gagggaccgc aaaagacatg accgagagcg tttcacaaaa
     2281 ggaaacggtc ctgcctgtcc tggaaaggac gttttccgcg ttcgaccggg cgatggatga
     2341 attgcgtcct cgcttgacgg tggaggagat cggtgtcctt acatttgtct cgaaggggat
     2401 cgcgcgcgtc aaaggacttc ccacggtgag atcgggagaa ctccttcttt tctccggcaa
     2461 cattcagggt attgccttta acacggacct cgaggagatc ggggtcgttc tcctcgatca
     2521 gggcgcaacc ctcggtgccg gctccctcgt gcggaggacc ggcagggtgc tggatgttcc
     2581 cgttggcgag gcccttatcg gcagggtgat cgacccgcag gggcgcgtcc ttgacggtca
     2641 gggtcccctt cgctccacgc ggcgccgtcc catagaaaag gccgccccgg ccatcctgga
     2701 caggctgccc gtgaccgttc ccctcgagac gggtctcaag gtgatcgacg cgctgatacc
     2761 tataggaagg gggcagcgcg agcttatact gggagatcgt cagacgggga agacggcaat
     2821 cgcggttgat gccatgatca atcagggggg caagggggtg gtctgcatct attgcgccat
     2881 cggcaagcgg ggctccgccg tggccaaggt tgtggccgac cttaaccagt acggggccat
     2941 gggtcattcc atcgttgttg tggccacgga ggagcatccg cccggtctac agttcacggc
     3001 gccctacgcg gcgatgacga tagcggagtt ccttatgggt aggggaaggg acgtgctggt
     3061 ggttttcgac gacctcacgc gtcatgcccg ggcttaccgg gagctctccc ttttgcttcg
     3121 caggcctccg ggcagagaag cctttccagg cgacatcttc tatgttcact cgcggcttct
     3181 tgaacggtcc acgcatctga aggaggagtt gaggggaggt tcgatcaccg ccctgcccgt
     3241 tgtggaaacg gaggcccaga acatctcagc ctacatcccc accaacctga tctcgataac
     3301 cgacgggcag atctacctct ctccagatct ctttcaaaag ggtgtcctgc cggcggtgga
     3361 cgtggggaga tcggtgtcga gggtgggtgg aaaaacgcaa ttccacgcgt accgggttgt
     3421 ggcaggtgat ctgcgccttg cctattcgca gttcgtggaa ctggaatcgt ttgcccggtt
     3481 cggcacgcgg ctcgatgagg aaacaaggaa gaccctggag cgcggtcgcc gggtcaggga
     3541 agttttgaaa cagccgcagt attccccaat gaag
//
LOCUS       DAOQTR010000201         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29791_length_3568_cov_2.964987, whole genome shotgun sequence.
ACCESSION   DAOQTR010000201 DAOQTR010000000
VERSION     DAOQTR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29791_length_3568_cov_2.964987"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2093
                     /locus_tag="PKN85_05835"
     CDS             <1..2093
                     /locus_tag="PKN85_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT43961.1"
                     /translation="RITAEEYKAIERKWHGAREFPTHYLRNAGIAAALLAALLLGLAI
                     WNRALRRAVRRKTAELKREMRLSEERAEALRESEERFALFLEHCPIMVFLKDKDNRAI
                     ALSRSFEKLLGRPLSEMLGKTSEELFPPELARSMRADDEEILRTGNLRTVEETFEGRS
                     YITCKFPLHRPDKTVLLGGFTIEITDRKRFEEALKKSEQQYRDVVNNANSIILRWDGA
                     GNIIFMNPYGLGFFGYREEELIGRNVVGTIVPELESASSRDLVSLMEEIQLNPDAFKS
                     NENENMRKNGERVWVSWTNKAVTNENGECVEILSIGKDVTEKRLLEAQLLQAQKMEAI
                     GTLAGGIAHDFNNLLMGIMGYASVMLLTTKPTDPNYEKLRSIEDQIRSGADLTRQLLG
                     FARRGKYEVKPTDLKDLIERCVDMFGHTNREITIHKGFPEDLCATEVDRSQMEQVFLN
                     LFVNAAQAMPLGGDLFVDARNVSFRDTRIVPKEGLYARISVTDTGVGMDEATKARIFD
                     PFFTTKDLGIGSGLGLASAYGIIKNHQGAITVDSKPAKGTRFTIYIPASQKPVKEEEP
                     AAAEVVRGAGTVLLVEDQPAVAETAREMLEALGYDVHVAGSGGEALELFARHRDEVSL
                     VILDMIMPGLSGSETYDRLKEMDPDIKVILCSGYSIEGQAAKILERGCNSFIQKPFTI
                     TELSEKLMEVLGRS"
     gene            complement(2090..2332)
                     /locus_tag="PKN85_05840"
     CDS             complement(2090..2332)
                     /locus_tag="PKN85_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43962.1"
                     /translation="MTTEEEKLVTRYADQAFRGTTIRQEYPVCECGKIFSEKNLCDAP
                     GVFFRSVDVFGKTYTLIEPVCPICKRKIPASFNILN"
     gene            2699..>3568
                     /locus_tag="PKN85_05845"
     CDS             2699..>3568
                     /locus_tag="PKN85_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT43963.1"
                     /translation="MSGVFGIVSKRNCASNLLYGTDYHSHMGTEYGGMAVLGDRFHRS
                     IHDISKSQFKSKFFEEYKEMKGNSGIGVISDRDAQPLIIGSKFGTFAIVSSGIVENTN
                     ELAKELLDSGETFSEMSGGGINSVELIAKLITQGKTLIDGIRGVYERIEGSASILLLT
                     ENGVWAARDRLGRTPLVVGEKDGDHAVATEDCSFLNLGFRRVRFLDPGEIVSIRPSGL
                     QSELPGTDRNQICAFLWIYTGYPASSYEGINVEVVRERSGRFLAKGDKVDADLVAGVP
                     DSGVGHAIGYAMES"
BASE COUNT          826 a          982 c         1105 g          655 t
ORIGIN      
        1 ccaggataac ggcggaggaa tacaaggcga tagagcggaa atggcacggt gcccgggagt
       61 tcccgacaca ctatctccgg aacgcaggca tagccgccgc gctccttgcc gccctcctcc
      121 ttggcctcgc gatatggaac cgggcactgc gacgcgccgt tcgccgtaaa acggcggagc
      181 tgaagaggga gatgaggttg agcgaggaaa gggcggaggc cctccgggaa agcgaggaac
      241 gattcgccct cttcctggag cattgcccta tcatggtctt cctcaaggat aaggacaatc
      301 gcgccatcgc cctgagccga agcttcgaga agcttctcgg gcgcccctta agcgagatgc
      361 tggggaagac gtcggaggag ctgttcccgc ccgaactcgc ccggagtatg cgcgccgacg
      421 atgaagagat cctccggaca gggaatctga ggacagtcga ggagacattc gagggacgca
      481 gttacataac ctgcaagttc cccctgcacc ggcccgacaa gacagttctt ctggggggtt
      541 ttaccattga gatcacggac cgcaagaggt tcgaagaggc tttgaagaaa agcgagcagc
      601 agtacaggga cgtggtgaat aacgcgaaca gcatcatcct gcgctgggac ggggcgggca
      661 atatcatctt catgaaccct tacggcctcg ggttcttcgg gtatcgcgaa gaggagctga
      721 taggaaggaa cgtggtggga accatcgtgc ccgagcttga atcggcaagc tcgagggacc
      781 ttgtgtccct catggaggag atacagctga atcccgacgc cttcaagagc aacgagaacg
      841 agaacatgag gaagaacggg gagcgcgtct gggtctcctg gacgaacaag gccgtcacga
      901 acgagaacgg cgagtgcgtg gagatcctga gcatcggcaa ggacgtgacg gagaagagac
      961 tcctcgaggc acagctcctt caggcgcaga agatggaggc catcgggacc cttgccgggg
     1021 gcatcgccca tgacttcaac aacctcctca tgggcatcat gggctacgcg tcggtcatgc
     1081 ttctcacgac gaaacccacg gaccccaact acgagaaact gaggagcatc gaggaccaga
     1141 taaggagcgg cgcggacctc acgaggcagc ttctcggttt tgcccggcgg gggaagtacg
     1201 aggtgaaacc cacggacttg aaagacctca tcgagcgttg cgtcgacatg ttcgggcata
     1261 ccaacaggga gataacgata cacaagggat tcccggaaga cctctgcgcc accgaggtcg
     1321 accgcagcca gatggagcag gtcttcctca acctttttgt caatgccgcg caggcgatgc
     1381 cgctgggcgg cgacctcttc gtcgatgcgc gaaatgtctc cttccgcgat acccggatcg
     1441 tgccgaagga agggctctac gccaggatat ccgttaccga cacgggcgtc ggcatggatg
     1501 aggcgacgaa ggcccgcata ttcgacccgt tcttcacgac caaggacctc ggtatagggt
     1561 ccggcctcgg cctggcatcg gcgtacggta tcataaagaa ccaccagggg gccataaccg
     1621 tcgacagcaa acccgccaaa gggacccggt tcaccatcta cataccggca tcacagaaac
     1681 ccgtgaaaga ggaagagcct gccgccgccg aggtggtccg gggagcggga acggtgcttc
     1741 tcgtcgaaga ccagccggcg gtggccgaga cggcgcggga gatgctcgag gccctgggct
     1801 atgacgtcca cgtggcggga agcggcggtg aagctctgga actctttgcc aggcacaggg
     1861 acgaggtgag cctcgtgatc ctcgacatga tcatgccggg gttaagtgga agcgagacct
     1921 atgacaggct gaaagagatg gaccccgaca tcaaggtgat cctttgcagc gggtacagta
     1981 tagaaggaca ggcggcaaag atactcgaaa ggggctgcaa ctctttcatc cagaaaccct
     2041 tcaccatcac cgaactatcg gagaaactga tggaagtcct gggcaggagc tagttcagga
     2101 tgttgaagct tgccgggatc ttacgcttgc atatggggca gacgggctcg atgagcgtgt
     2161 acgtcttccc gaaaacatcg acgctcctga agaacactcc cggagcgtcg caaaggttct
     2221 tttcactgaa gatcttgccg cattcacaca cgggatattc ctggcgtatc gtcgttcccc
     2281 tgaatgcctg atcagcatac ctggtgacca atttttcttc ttccgtcgtc atggctttaa
     2341 tataatctcc ttatccctgt ttggcaacct tgcctctggt gccgcgacat tctcgacatt
     2401 gcctcatggg cgccgtccta cagatatggt cggtacgggg agggagaaac ttaaagaaga
     2461 cggttgcggg ttgcgggttg cgggaaagaa gacggtgaaa gccggcgcac gtcaacacgg
     2521 ctgacctgtc agggtctcaa cgcttcgtcg tttcggcgag ggaggacgta aaaggcctgc
     2581 cgggatacgg gcgggatggt gtgcattcct gtggaatttt ccggggaatc tatatatcat
     2641 atccttgcca tttaaacgcg ttatcgacaa cagatcagga tgccatcagg gagggcgtat
     2701 gagtggagtt ttcggcatag tatcgaagag gaattgcgcg agcaacctgc tctacggtac
     2761 cgattatcac tcgcatatgg gaactgaata tggggggatg gccgtactgg gagaccggtt
     2821 ccatcgcagc attcacgata tcagcaagtc gcagttcaag tcgaaattct tcgaggaata
     2881 caaggaaatg aagggcaatt cgggcatcgg ggtcatcagc gaccgggacg cccaacccct
     2941 tatcatcggc tccaagttcg gcaccttcgc catcgtttcc tcgggcattg tggagaatac
     3001 caacgagctc gccaaagagc ttctcgacag cggcgagaca ttcagcgaga tgtccggcgg
     3061 cggcataaac tccgtcgagc tcatcgcgaa gctgatcacc cagggcaaga ccctcatcga
     3121 cggtatcagg ggcgtatacg aacgcattga aggttccgct tccattctgc tcctcacgga
     3181 aaacggggtc tgggcggcca gggaccgcct gggcagaacg ccgctcgtcg ttggagagaa
     3241 ggacggcgac catgccgtcg ccaccgagga ctgctccttt ctgaacctgg ggttccggag
     3301 ggtcaggttc ctcgaccccg gagagatcgt gagcatccgc ccgtcgggtc ttcagagcga
     3361 gctcccgggc acggaccgga accagatatg cgccttcctc tggatataca cgggatatcc
     3421 cgcctccagt tacgaaggga tcaacgtgga ggtggtccgg gaacgctcgg gaaggttcct
     3481 ggcaaagggc gacaaggtgg atgccgatct cgtcgccggc gtccccgatt cgggcgtcgg
     3541 ccatgccata ggctacgcca tggaatcc
//
LOCUS       DAOQTR010000202         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_29862_length_3562_cov_3.577987, whole genome shotgun sequence.
ACCESSION   DAOQTR010000202 DAOQTR010000000
VERSION     DAOQTR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29862_length_3562_cov_3.577987"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PKN85_05850"
     CDS             <1..739
                     /locus_tag="PKN85_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNT43964.1"
                     /translation="FRNVPLIAIAGLGGVGGGTFLNLVRSGAKRFRLAENGVFDVPDM
                     NRQAAAFASTLGRPKIEVYEEFARGINPGVELELFPEGLLAGNIERFFEGSDVYVGVI
                     DVEKGADAKAMTPEMLKRFNIPLFSAMVVGFGTMLVAHHPEGMMPDEFWGLVRKRSSR
                     TRFPSFLSRRFGSTVAERVENSFAAGKGPSTSVGGAFAGAVLASEVMAYLLRGTGLVD
                     REAIFAPRFMVVDPMGPTMEVVDVTAH"
     gene            1111..2439
                     /locus_tag="PKN85_05855"
     CDS             1111..2439
                     /locus_tag="PKN85_05855"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT43965.1"
                     /translation="MSGIFGFIGRGNGFAEVRSGLENLSHRGQESWGIVSALKDGSFQ
                     EARGLGSIFQASGLSRSYFGSLAIGQVRYPTTGETNERNSQPIVGSFGKEKIAVVHNG
                     HIPGYKRLVEELGGLFQTETDTEVILQEIARMEGEDLLEKMEKTLRFLGRQAAFSIII
                     LHDGKLIAARDPFGFRPLSIARRGEEGDYTWALASETCAFHGQFEWIGDVEPGQMVVL
                     DGESMKTVSFADPDPHPCIVECLDYASPASRVFSRGCYEFREKIGAELAKTETEKADI
                     VVPIPRAAIPAALGFHDTAGIPFKVAISTVGEIGRVFIISKEKDRFAQAEKKFQVNGE
                     LVKGKEVFLIDSLLVRGSTAMVLIPKLREAGATKIHMRLTAPPPRFPCHMGMAMAKPG
                     ELLAGNRDDEQLRKLIGADTFRYMTIEELKGLMGTQFCDACFTGKYPFSV"
     gene            3026..>3562
                     /locus_tag="PKN85_05860"
     CDS             3026..>3562
                     /locus_tag="PKN85_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43966.1"
                     /translation="MKRFIAVMIGMALVCAGLGSAFAAGYSFNALDYPGASETRAYGI
                     NDWGVVVGSHQGAGEVYGHAFVLRGDTWTSFDDPDAAFGTSPRGINDWGKIAGTFGNS
                     IGDVSGFTLAFGVRRRLDYPTAAHTFLGGVNGLGTVVGYYMIPSTRSFVLKGTTYTLL
                     DYPPTTDDPENPTGWLRAH"
BASE COUNT          707 a         1027 c         1096 g          732 t
ORIGIN      
        1 gttcaggaat gttcctctca tcgccattgc cggcctcggg ggggtgggcg gaggaacatt
       61 cctgaacctt gtccgctccg gggcgaagcg gtttcgcctt gccgagaacg gcgtcttcga
      121 cgttcccgac atgaaccgcc aggccgcggc ctttgcgtcg accctggggc ggccgaagat
      181 cgaggtttac gaagagtttg cacgaggtat caaccccggg gtcgagctcg aactcttccc
      241 tgaagggctg ctcgcaggca atatcgagcg gtttttcgag ggcagcgacg tctatgtcgg
      301 cgtcatagac gtcgaaaaag gtgcggacgc caaggcgatg accccggaga tgctcaaacg
      361 cttcaatatt cccctctttt cagccatggt ggtcggcttc ggcacgatgc tcgtggccca
      421 ccatccggaa ggcatgatgc ccgatgagtt ctgggggctg gtgaggaaaa ggtcctcccg
      481 cacgcgtttc ccatccttcc tgtcccgcag gttcggatcg accgtggccg aaagggtgga
      541 gaactccttc gcggccggca agggtccgtc aacgagcgtc ggcggcgcct tcgcgggtgc
      601 ggtcctcgct tccgaagtta tggcttacct tcttcggggg accggccttg tcgacaggga
      661 ggccattttt gcccccaggt tcatggtagt cgaccccatg ggaccgacca tggaagtggt
      721 tgacgttacg gcacactgat ctgttcgcct gctgaccgcg atccatctcg cctcacaggc
      781 gtccctattc cccagcagcc gctggcaaca agatctcggg gcctcgcgag aacgaggttc
      841 cggggttcac atgtttgcgc ttgacacgct tccggaagca cccgaaccgg tgccgatcct
      901 ttgcttttgt ccggtgtttc ggggttttgt tgttgcatat ctgcgggaat atgcggaaaa
      961 ttagggtaag cacgcggtcg aggacatccg caacagttca cgcggcattc cgatggtcac
     1021 gcgaagacag ggacagcgcg tcagtggtcg gtgccccgtt ggaccatgcc ggttgagcag
     1081 gccgggtgct gtgagagctt gggaggtatt atgagtggaa tcttcgggtt tatcggcagg
     1141 ggtaacggct tcgccgaggt gaggtcggga cttgagaatc tctcccacag gggacaggaa
     1201 agctggggca tcgtctcggc cttgaaggac ggttctttcc aggaggcccg ggggttgggg
     1261 tccatcttcc aggcttccgg cctcagccgg tcctatttcg ggtcccttgc gatcggtcag
     1321 gtccgttatc ctaccacggg agagaccaac gagaggaaca gccaacccat cgtcggaagc
     1381 ttcgggaagg agaagatcgc cgtcgttcac aacggccata tacccggata caaacggttg
     1441 gtggaggaac tcgggggcct cttccagaca gagacggaca ccgaggtgat cctgcaggag
     1501 atcgcccgga tggaaggcga agacctcctt gagaagatgg agaagacctt gaggttcctg
     1561 ggccggcagg cggccttcag catcatcatt ctccatgatg gcaagctcat cgccgcacgg
     1621 gaccctttcg ggttcaggcc tctctccatc gccaggagag gtgaggaggg cgattacacc
     1681 tgggcgttgg cctcggagac atgcgccttt cacggtcagt tcgagtggat aggcgatgtc
     1741 gagccgggac aaatggtggt cctcgatgga gaaagcatga agaccgtctc cttcgcggac
     1801 cccgaccccc atccctgcat cgtcgaatgt ctcgattacg cctctccggc gagccgggtc
     1861 ttttcaagag gctgttacga atttcgggag aagatcggcg ctgagctggc aaagaccgag
     1921 accgagaagg ccgacatcgt cgttcccatt ccgcgagccg ccattcctgc cgccctgggt
     1981 ttccatgaca cggctggaat acctttcaag gtggccatca gcaccgtggg cgagatcggc
     2041 agggtgttca taatctccaa ggaaaaggac cgctttgccc aggccgagaa gaaattccag
     2101 gtcaacgggg agctcgtgaa aggcaaggaa gtcttcctca tcgattcgct tctggtccgg
     2161 ggttcgacgg ccatggtcct catcccgaaa ctgcgtgagg caggggcgac aaagatccac
     2221 atgcgcctga cggctccgcc cccgcgcttt ccctgtcaca tgggaatggc aatggccaaa
     2281 cccggggagc ttctggccgg gaaccgggac gacgagcaac tcagaaaact gataggcgcg
     2341 gacactttca gatatatgac gatcgaggaa ctgaaggggc tgatggggac gcagttctgc
     2401 gatgcctgct ttaccggaaa gtatcctttt tcggtgtgaa aaggtcacga acgatcacgg
     2461 tcagacctcc tgtcggtcac aggaagacgc gatctcgcga gcggccgcgg taagatctcc
     2521 ctgcccggca cctccagcct ttccggtgtt cgggaacctg tcccgcaccg gcatcgatgc
     2581 cagccgatcc tgtggtcccg cggcatctct tcccgtagag accccatgcc gtattactcc
     2641 gcctctccat ggttctccca caatagccag gtgaattgat caacgggacg gcgctcccgc
     2701 gaggatacgc tccggacgat tgaccggacc cggagacgag cacccgtcga gagcaagggc
     2761 ccgggagtcc cggtcccggt gacagacacg gaaaatgcga cagggaaggt ggtgaaatta
     2821 ccacaaaaaa aatgtccctt tggcgacaga cacgaacggg cactccatgt agcatgtaat
     2881 cagtcttttg cctgcatttt gttattcacc ctgagatacg acacctgcag cgaacacctc
     2941 accttgttct gcttacgcga ccatctcccg cgaaacgccc ccgcgccacc tgtggaacaa
     3001 agattcacaa aaggagggga aacagatgaa acgttttatt gcggtcatga tcggtatggc
     3061 tcttgtgtgt gccggactgg gcagtgcttt cgccgccggt tacagcttca acgctctcga
     3121 ctatcctggg gcttcggaga cgcgggcgta tggcatcaac gattgggggg tcgtcgtcgg
     3181 gtcccaccag ggcgccgggg aggtctacgg ccatgctttt gtcctgagag gtgatacgtg
     3241 gacatctttc gacgatcccg atgccgcctt cgggaccagt ccgcgaggta tcaatgattg
     3301 gggaaagatc gctgggacct tcgggaacag cattggggat gtcagcggtt ttacccttgc
     3361 tttcggggtg cgcagacgcc ttgattatcc caccgcggcg cacaccttcc tcgggggtgt
     3421 caatggcttg ggcacggtgg tcgggtacta catgatccct tcaacgcgca gtttcgtgct
     3481 gaaaggtacg acctacaccc tgcttgacta tcctcccacc actgatgacc cggagaaccc
     3541 gacgggatgg ctccgtgccc at
//
LOCUS       DAOQTR010000203         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_30224_length_3532_cov_3.152430, whole genome shotgun sequence.
ACCESSION   DAOQTR010000203 DAOQTR010000000
VERSION     DAOQTR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30224_length_3532_cov_3.152430"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKN85_05865"
     CDS             complement(<1..246)
                     /locus_tag="PKN85_05865"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNT43967.1"
                     /translation="MMERSGFAVWFTGLPSSGKTVTAEKTCELLYERGVPMVFFDSEE
                     LRKTLIQRPVYTDEERDLFYSVVVYLVECITERGVNVA"
     gene            complement(482..724)
                     /locus_tag="PKN85_05870"
     CDS             complement(482..724)
                     /locus_tag="PKN85_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43968.1"
                     /translation="MARKLSARRRIPKSKFDAQETHICPRCNAFLVRERFYGMYAHIW
                     VWRCIKCGEIIDDVVLYNRRLLKKAAKGVDIMTLIR"
     gene            complement(990..2315)
                     /locus_tag="PKN85_05875"
     CDS             complement(990..2315)
                     /locus_tag="PKN85_05875"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT43969.1"
                     /translation="MIDDEVRWCELLRETLASEGFDVAVAGDSALASQLIHQVDYDVI
                     LTDLRMDGKNGLQLLEETKKVAPTTPVILITAFGSIDSAVQAMKMGAYDFITKSGDMS
                     ELILTVEKALETKSLRKEVIGLKRKLESQYSFHKMIGKSPLMQKIYQLIEMVCDTSGN
                     VLITGESGTGKELVAKAIHYNGVRKNGPFMAVNCAAIPENLLESELFGYRKGAFTDAK
                     IDKEGLVVGAAGGTLFLDEVTEMPPSLQAKILRVIEEREVRPLGGTTSYPTDVRIIST
                     SNRDIQSRIDQGLFRSDLYYRLRVIDIDLPPLRGRKEDIPLLVRHFLNTFNKELKKKV
                     THVSTEAMRILMDYSWPGNVRELENVVQRAVTLCHGETVVPDDFPALMTRTNGRSFLD
                     RGPEKGRTLEELETEYIREILAEVGGNRSRAAEILGIDRKTLYRKLKNE"
     gene            2633..2818
                     /locus_tag="PKN85_05880"
     CDS             2633..2818
                     /locus_tag="PKN85_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43970.1"
                     /translation="MVAMPKQGEKVRSKFNGREFIVELVGERMIVLQERREAIRFVTT
                     IDNLRSFYELDKDRSAP"
     gene            3160..3483
                     /locus_tag="PKN85_05885"
     CDS             3160..3483
                     /locus_tag="PKN85_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43971.1"
                     /translation="MKRSVFSTTMVIFVLLLFASTAVSMNHGMTKSMTGKVIGVDENG
                     KGIAISSKAGGQEIVAGAIVTDATVVHVKGKKANIMDIKAGDTVTLTYSYENNDLYAK
                     KVVKR"
BASE COUNT          755 a          999 c          975 g          803 t
ORIGIN      
        1 ggccacgttg accccccttt ccgtgataca ctccacgaga tagaccacaa cgctgtagaa
       61 caggtccctc tcctcgtcgg tatagacagg gcgctggatc agcgtctttc gaagctcttc
      121 ggaatcgaag aacaccatag gcacgccacg ttcgtacagc agctcacagg tcttttccgc
      181 tgtcaccgtt ttacccgatg acggcaaccc ggtgaaccac accgcgaatc ccgaccgctc
      241 catcatccca ccccctgttc tgtgtcttca tttgtcaacc cgatttcgtt ccgacccggg
      301 aacgaactga gcgctgagct tggatgaaag tgacgagggg tgcggcccgc cctctcgcaa
      361 gacgggaact ccggcagtac cgccccctct tgaaacgcgc tggttttgat cacacccacg
      421 atggtttacc cggaaaacac aagctccgcg tgacgcatgg tacgcccttg ccgatcccgt
      481 gtcaccggat cagcgtcatt atgtctaccc ccttggcggc cttcttgagc agtcgccggt
      541 tgtagagcac cacgtcgtct atgatctcgc cgcattttat gcatcgccaa acccagatgt
      601 gggcgtacat gccgtagaac ctttcgcgga ccagaaaggc attgcagcgc ggacagatgt
      661 gtgtttcctg agcatcgaac ttgctcttcg gtattcgcct gcgtgcgctt agtttccttg
      721 ccatggttcc tccttccttg ctgtggagcc atcaattttc taaggatgtt gcagcttcgc
      781 tgggacactc acccggaata tgcaacttgc cgcgagtctg caatttgcac gccatggcgt
      841 cgaaaacagg cgcaaatgaa cgccagatag tctattgcga tgaaggggaa tgtccggccg
      901 aaaccgggat gtctttcggg taatggggca aaaggtcccc atcgggcagg cattgtgccc
      961 cagtgctccc gggcaccggg gatctccgct tattcgttct tgagtttgcg atagagcgtc
     1021 tttctgtcta tgccgagtat ctccgcggcc cgggagcgat tacccccgac ctcggccagt
     1081 atctcccgga tgtattccgt ctcgagctcc tccagggtcc ggcccttttc cggaccgcgg
     1141 tcaaggaagg acctgccgtt cgtccttgtc atgagcgcgg ggaaatcatc ggggacgacg
     1201 gtctcgccgt ggcacagcgt gacggccctc tggaccacgt tctcgagctc cctcacgtta
     1261 cccggccatg aatagtccat caggatcctc attgcctccg tggagacatg ggtgaccttc
     1321 tttttcagct ccttgttgaa tgtgttcagg aagtgcctga cgagaagagg aatgtcctct
     1381 tttctccccc tgagcggagg aagatcgatg tcgatgacac ggagccggta ataaagatcg
     1441 cttcggaaca gcccctggtc tatccgtgac tggatgtcgc ggttggacgt ggagattatg
     1501 cggacgtccg tgggataaga ggtcgtgcct cccagcggcc gcacttccct ctcctcgatg
     1561 acccttaata tcttggcctg aagcgagggg ggcatctccg tgacctcgtc gaggaaaagg
     1621 gtgccgccgg cggcccccac gacgagtccc tccttgtcga tcttcgcgtc cgtgaaggct
     1681 cccttccggt atccgaagag ctcgctttcc agcaggttct cggggatggc ggcgcagttc
     1741 acggccatga aaggtccgtt cttcctgaca ccgttgtagt ggatcgcctt tgccacgagc
     1801 tctttcccgg tgccgctctc acctgttatg aggacgttgc ccgacgtgtc gcagaccatt
     1861 tcgataagct gatatatctt ctgcatgaga gggctctttc cgatcatctt gtggaagctg
     1921 tactgggatt ccagcttgcg tttcaggccg atgacctctt ttctgaggga cttcgtttcc
     1981 agggcctttt caacggtcag gatgagctcg ctcatgtctc cgctcttcgt tatgaaatcg
     2041 taagcaccca tcttcatggc ctggacggcc gaatcgatgg acccgaaggc ggtgatgaga
     2101 atgacgggtg tcgtaggggc aaccttcttc gtctcttcaa gaagctgcag cccgttcttc
     2161 ccgtccatcc tgagatcggt gaggatgacg tcgtaatcga cctgatgtat gagctgggag
     2221 gccagggcgc tgtcaccggc aacggcaacg tcaaaaccct cagaggcaag cgtctcccgc
     2281 aacagctcgc accatctcac ctcgtcgtcg atcaccagta tcctgtgaaa cctgtccttc
     2341 aggggaacac ctccttgaag accggcaacg cccttttcac accgggggac gaccctcgta
     2401 cgcggaccgg gaagaacctt cagattccat tctaccctat tatgatcgaa tcgtaaaggg
     2461 cgggctgggg cataacacca ctatgagggg caaaaaaccc cctgtctgcc tgcctccgca
     2521 aagatgcttt caccgtgttt tctcttgtcc tttcaacaaa aagggtatgt ggtacacgtt
     2581 ttgcttgagc aggaggacaa gaagtttttc gcacgacaac ggagggagat gcatggtagc
     2641 tatgcctaaa cagggagaaa aggtcagaag caagtttaac ggaagggaat tcattgtcga
     2701 actggtgggt gagagaatga tagtgctcca ggaaagacgg gaagccatac gcttcgtcac
     2761 gacgatcgat aatctgcggt cattctacga actagacaaa gaccggagcg ctccctgatg
     2821 tcgtggaaac aacacaggca aaaaggaggg accgtatgaa agggcgagtt tattccgtta
     2881 tgtggacgat tttcctgctc cttcttctcg cgtcggcggt gatgtcctga aatccctgag
     2941 agcccgtgac tggatgtcac ggtcggatgc ggggatgatt catgaaggcg acagagcggg
     3001 tcggggcatt acgatcactg tcaggggcaa aaggcccctg ttcaccccgg tccttccgac
     3061 tcgttacggc gcaagcgctg cctggtagat gttttgcttc attttagtat acagggagct
     3121 tacagcatgc ccccaaatca cagaaagagg aggaaatcta tgaaacgatc cgtcttcagc
     3181 accaccatgg tgatctttgt tcttttgctc ttcgcatcga cagcggtgtc catgaaccac
     3241 ggaatgacaa agtcgatgac aggcaaggtt atcggtgtcg acgagaacgg aaagggtatc
     3301 gcgatctcgt caaaggccgg aggtcaggag atcgtcgccg gcgccatcgt aacagacgcg
     3361 acggtggtcc atgtcaaggg caagaaggcg aacattatgg acatcaaggc aggcgacacg
     3421 gtaactctca cgtattcata cgaaaacaac gacctttacg cgaaaaaggt cgtcaagagg
     3481 tgacgaaggg ctgacctgca ccccgggtcg ggacgaccgg ggtttcgcac gt
//
LOCUS       DAOQTR010000204         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_30461_length_3514_cov_3.589477, whole genome shotgun sequence.
ACCESSION   DAOQTR010000204 DAOQTR010000000
VERSION     DAOQTR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30461_length_3514_cov_3.589477"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PKN85_05890"
     CDS             <1..557
                     /locus_tag="PKN85_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HNT43972.1"
                     /translation="RLGGLVEVVDGRPLRFTSVISYEGSSVPPLEILMRSRMSDVLGA
                     VEGGSGLILGSYREIPENSVSDAKDLLERMAPHGFGGIVLFGSRGEPLLGISPTDGKV
                     GIVVLGGLNVNAALEQAGVGRGTQAMATLCDYPLLSSIEALERAYLPRKEESSLFLRY
                     LSRTRQESDDGYWSVLRALKQMTF"
     gene            complement(515..2389)
                     /locus_tag="PKN85_05895"
     CDS             complement(515..2389)
                     /locus_tag="PKN85_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT43973.1"
                     /translation="MSTDEEEMVLDQLCRIMIGREERIVALAKKYFTLEDLLAIKARL
                     IGTGYIGGKTVGMLLARKILSRDMSFDWQRVLEPHDSFFIGSDVFYTYLVENGWWKLR
                     MEQKTRDGYFEVAKSLRESMLEGSFPDEIEEQFWRLIEYFGQSPIIIRSSSLLEDAFG
                     NAFAGKYESVFCVNQGTPEERYKQFGEYVRHVYASTMNEDALAYRLQRGLDKHDEQMA
                     LLVQRVSGSYHGSYFYPDLAGVGISYNTFVWRRDMNPKAGMLRLVAGLGTRAVNRVEH
                     DYPSIVALDLPLLRPHSGPSDMKKYSQHNGDVLNVETNVLETIPVTDIITGDMKADRE
                     LMGTRDLEAAEFLRSRGLEPRESWIVTFDRLMSETPLPTIMQRMLKTLETSYQYPVDI
                     EFTINFTASDAFQINLVQCRPLQTKGEGKRVRIPRKVPADRILFQSSGNFMGSNITQG
                     IKRIIVVDPDGYSLLTLSEKYDIARLIGRINRQIPGQEEMPTILAGPGRWGTTTPSLG
                     VPVRFSEINNIAVLVELAHGTGLGSVMPELSFGTHFFQDLVETGVFYVAIFPDREGSS
                     FSRRIVDRKYNLLSILVPESSRYEGIVTVVDTTGDDMRLLADVVTQKVICFSALKTDQ
                     "
     assembly_gap    2500..2509
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2510..3250)
                     /locus_tag="PKN85_05900"
     CDS             complement(<2510..3250)
                     /locus_tag="PKN85_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43974.1"
                     /translation="MARILARESAGIAGIDEIIDGLRTGDNVVWQVDSIDDYLSFAKP
                     FVERSVRDGKKVVYMRFAGHRPVIEGNENVRVHNLDARSGFESFTAQVYNIITSEGRD
                     VHYVFDSLSDLLSAWATDLMIGNFFTIACPYLYQLNTIAYFALLRESHSFKTVARIRE
                     TTQVLIDVYNVDDKIYVHPLKVRGRHSPTMFLPHRKSEDRFLPVTSSVDTGRLLTRIS
                     VKGTDLAKRNLDHWDRFFIRVADLLKEKV"
     gene            complement(3289..>3514)
                     /locus_tag="PKN85_05905"
     CDS             complement(3289..>3514)
                     /locus_tag="PKN85_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNT43975.1"
                     /translation="MAACAMLAALGAADELRLHVNAALNVGCDPRKLAEVFFQLAPYG
                     GMPLVNKALEVFREVLKGRGEWETFTKGGK"
BASE COUNT          613 a         1015 c         1129 g          747 t
ORIGIN      
        1 ccaggctcgg aggtctcgtc gaggtggtgg acggacgtcc tttgcggttc acctcggtga
       61 tcagctatga ggggtcttcg gtccctcccc tcgagatcct catgcgcagc aggatgagtg
      121 atgtgcttgg cgcggtggaa gggggatcgg ggctcatcct ggggagctac cgggagatcc
      181 cggagaacag cgtgtccgac gcgaaggatc ttctcgagcg catggcgccc cacggtttcg
      241 ggggcatcgt gctcttcggg agccggggag aaccccttct cggcatctct cccacagacg
      301 gcaaggtcgg catcgtggtg ctcggcgggc tcaatgtgaa cgcggccctc gagcaggccg
      361 gggtgggcag gggaacccag gcgatggcaa cgctctgcga ttatcctctc ctgagttcga
      421 tagaggccct ggaaagggcc tatctgccga gaaaggaaga gagctctctt ttcctcaggt
      481 atctttcccg gacccggcag gaaagcgacg acggttactg gtccgtctta agggcgctga
      541 agcagatgac cttctgagtc acgacatcgg cgaggagcct catgtcatct cccgtggtgt
      601 caacgacggt gacgatgcct tcgtaccggg agctctcggg aaccagtatc gagagaaggt
      661 tatactttct gtccacgatc cttctgctga aggatgaccc ttccctgtcg gggaagatgg
      721 cgacgtagaa tacccccgtt tcgacgaggt cctgaaagaa gtgggtgccg aaggaaagct
      781 cgggcatcac gctgccgaga cccgtgccgt gcgccagttc caccaggacg gcgatgttgt
      841 tgatctcgga aaaccgtacc gggactccca gtgagggtgt ggtggtgccc caccgtcccg
      901 gtccggcaag gatcgtgggc atctcctcct gaccgggtat ctgtctgttt atcctgccga
      961 taaggcgcgc gatgtcatat ttctccgaca gcgtcaggag gctgtacccg tcagggtcga
     1021 ccactattat ccgcttgatc ccctgcgtta tgttgcttcc catgaaattc cccgaggact
     1081 ggaagagtat cctgtcggcg ggcaccttgc gcgggatcct gaccctcttt ccctcgcctt
     1141 tcgtctgcag gggtctgcac tggacgaggt tgatctgaaa ggcgtcgctg gccgtgaagt
     1201 ttatggtgaa ttcgatgtcc acggggtact ggtagcttgt ctccagggtc ttgagcattc
     1261 gctgcatgat cgtcgggagg ggcgtttcgg acatgagcct gtcaaaggtc acgatccatg
     1321 actccctggg ctcaagcccg cgggacctca ggaactcggc cgcttcgagg tcgcgtgttc
     1381 ccatgagctc ccggtccgcc ttcatgtcgc ccgtgattat gtcggtgacg gggatcgttt
     1441 cgagaacgtt ggtctcgacg ttgaggacat cgccgttgtg ctgggagtat ttcttcatgt
     1501 cgctcggccc gctgtgaggg cggagcagcg ggagatcgag ggcgacgatg ctgggataat
     1561 cgtgctccac gcggttcacc gccctggtgc cgaggcccgc cacgaggcga agcatccccg
     1621 ccttcgggtt catgtcccgt ctccagacga aggtgttgta ggatatcccg acaccggcaa
     1681 gatcggggta gaagtagctg ccgtggtagg agcccgaaac cctctggacc aggagcgcca
     1741 tctgctcgtc gtgtttgtcg aggcccctct ggaggcggta ggccagggcg tcctcgttca
     1801 tggtgctcgc gtacacgtgc cgcacatact cgccgaactg cttgtagcgc tcctccgggg
     1861 tgccctggtt gacgcagaag acgctctcgt acttgccggc aaaggcgttg ccgaaggcgt
     1921 cctcgagaag gctgctcgac cgtatgatga tgggcgactg tccgaaatac tcgatgaggc
     1981 gccagaattg ctcttctatc tcgtcgggga aagaaccctc gagcatggat tccctgaggc
     2041 tcttcgcgac ctcgaaatac ccgtcccggg tcttctgttc catgcgcagc ttccaccacc
     2101 cgttctcgac gaggtacgtg tagaagacat cggaaccgat gaagaacgaa tcgtggggtt
     2161 cgaggacgcg ctgccagtcg aaggacatgt cacgggagag gatcttcctg gcgaggagca
     2221 tgccgaccgt tttccctccg atatagcccg tccctatgag gcgcgccttg atggcgagga
     2281 ggtcctcgag tgtgaagtac ttcttcgcga gggcgactat cctttcctcg cggccgatca
     2341 tgatcctgca caactggtcg aggaccattt cttcctcgtc cgtgctcacc ttttctttca
     2401 ggaggtcggc tcggcgcgcg caacggggcc cgcgtcgtcg gccggggact cgtcggtggc
     2461 cgagtcgttc acggcgacga ggtcagggcg ggcggcgtcn nnnnnnnnnc accttttctt
     2521 tcaggaggtc ggcgacgcgg atgaagaacc tgtcccagtg gtcgaggttc cttttcgcca
     2581 ggtccgttcc cttgacggag atgcgggtga gcaggcggcc cgtgtccacg ctgctcgtca
     2641 ccggcaggaa ccggtcctcg ctcttgcggt ggggaaggaa catcgtgggc gagtgtcggc
     2701 cgcggacctt gagaggatgc acgtagatct tgtcgtcgac attgtagaca tcgatgagca
     2761 cctgggtcgt ttcgcggatg cgggcgacgg tcttgaagga gtggctctcc ctgagcagcg
     2821 cgaaataggc tatggtgttg agctggtaca gataggggca ggcgatcgtg aagaagttgc
     2881 cgatcatgag gtccgtggcc cacgcggaga ggagatcgga gagggaatcg aaaacgtaat
     2941 ggacgtcgcg cccttccgag gtgatgatgt tgtagacctg ggcggtgaag gattcgaagc
     3001 cgctgcgcgc gtccaggttg tgcacccgga cgttctcgtt gccctctatc acgggcctgt
     3061 ggccggcgaa ccgcatgtag accaccttct taccgtccct gaccgaccgc tcgacgaagg
     3121 gcttcgcgaa agacaggtag tcgtcgatgg agtcaacctg ccatacgacg ttgtctcccg
     3181 ttcgcagccc gtcgatgatc tcgtcgatgc cggcgatgcc cgcgctttcc cgtgcaagta
     3241 ttctggccat gcaagaatcc cggctttaga gccgcctcct attggatcct acttcccgcc
     3301 cttcgtgaag gtctcccatt ccccccggcc tttgaggacc tccctgaaga cctccagcgc
     3361 cttgttgacg agcggcatcc cgccgtaagg ggcgagttgg aagaagacct cggcgagctt
     3421 cctgggatcg catccgacgt tgagggcggc gttcacgtgg agcctcagtt catcggccgc
     3481 tcccagggcg gcaagcatgg cacaggcggc catc
//
LOCUS       DAOQTR010000205         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_30464_length_3514_cov_3.259034, whole genome shotgun sequence.
ACCESSION   DAOQTR010000205 DAOQTR010000000
VERSION     DAOQTR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30464_length_3514_cov_3.259034"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1832
                     /locus_tag="PKN85_05910"
                     /pseudo
     CDS             47..1832
                     /locus_tag="PKN85_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            1745..>2198
                     /locus_tag="PKN85_05915"
     CDS             1745..>2198
                     /locus_tag="PKN85_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT43976.1"
                     /translation="MYSIRGLELDRLEAYTREIRDRFGKLPGIVDLDTSLEKGKPEVR
                     VFIDRDKAADLGIDVATVAETANFLLSGEVDITKFKDEARGRRYDVRVRLNPENRSDP
                     EDIGEIYVRSKEGKLVMLSNIVSLKEGGGPSVINRVDRQRAVTLFANLE"
     assembly_gap    2199..2208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2209..2824
                     /locus_tag="PKN85_05920"
     CDS             <2209..2824
                     /locus_tag="PKN85_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT43977.1"
                     /translation="ARSELDDISSKVLTSGYSGSYKGQADTMKESFGYLLFAAILGVI
                     MAYMVLAAQFESFVHPFTVLLSMPFSLIGAFGALFVTGKTLNIFSIIGLILLMGLVKK
                     NAILLVDYTNILRERGAVRRDALLEAGPVRLRPILMTTFAMIMGMMPIALGIGEGAET
                     RSPMALATIGGLITSLLLTLVVVPVAYDLFDEMKERYFPRKDGR"
     gene            2824..3114
                     /locus_tag="PKN85_05925"
     CDS             2824..3114
                     /locus_tag="PKN85_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43978.1"
                     /translation="MKMLIIVYNDVVDEELVAMLKKAGVSGYTKWKEALGEGPGTGPK
                     LATNIWPGKNNVIAVVVNDEELPAVSGLLRDLKGSHPKGGIKAFILEVEGTI"
     gene            3202..>3514
                     /locus_tag="PKN85_05930"
     CDS             3202..>3514
                     /locus_tag="PKN85_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43979.1"
                     /translation="MSLFFVTFFTLYTALHAYAFIKAWYAFRFRPLTGCILALFLLFC
                     VFMPLFVRALEQGGQESIARVFAYAGYIWMSAIFIFFATSLVIDIGRLCVIIASSLFK
                     VG"
BASE COUNT          749 a          975 c         1034 g          746 t
ORIGIN      
        1 gaaagacatc gacctggccg tgcaggatgt cagggaaaag atatccgtga tacggtccaa
       61 gctgccccac gatatcgacg agcccattgt ggagaaagtc gaccccgacg cgacacccgt
      121 cctgtggctt gccctgcacg ggcagagatc gatgcgtgat ctttccacgt acgcggacga
      181 ggtcctcaag gaacagcttc agcggataaa cggggtcggt gcgatccgcc tgtatggaat
      241 gaggctccgc caggcccgca tatggctcga caacgacagg ttgagggcct atggggtgac
      301 ggcccatgac gttatgaagt gcctccagag ggagaatgtg gaactgccgg gaggaaggat
      361 agagagcgcg accaaggaat acaccatcaa ggtcaagggc gagttcccga cgatccagga
      421 cttcaacgag atggtggtgg gctattacaa gggaacgccc gtgcgcctca gagacatcgg
      481 ccgcgcggag gacggcatgg aggagaagcg gaccatttcc cggtataatg gagctcccac
      541 catcgccgtc ggcatccaga agcagtccgg gacgaacacc gtcgaggtca tagagaggat
      601 aaagaaggaa ctggaagaca tatcacgact gctgcccaac gggatgagcc tggacatcgc
      661 cttcgatcag tcgagcttca taaagaggtc cataaacgat gttcgtcatc acctcatata
      721 cggggggttc ttcgctattc tggccgttct tctcttcctg agaaacttca gaacaacgat
      781 aataagcgcg ctggccatcc cggcctccgt catctccact ttcgcgatca tgaacgcctt
      841 cggcttcacc ttcaacaaca tgtccatgct ggccctgtcc ctttccatcg gcattctcat
      901 agacgacgcc atcatcgtca tcgagaacat ccagcgccat atcgaagacg gaatgacgcc
      961 cagggaagcc gcctttttcg ccacttccga gatcggcctc gccgtttcgg cgacgacgct
     1021 ggccatcatc gtcattttca tacccgtggc cttcatgaag ggtatcatag gacgtttctt
     1081 cttccagttc ggtatgaccg tggtctttgc cgtgatggtt cgtttccttc acgctgacgc
     1141 ccatgctgtc ctccctctac ctgaagggta gggacagtgt ccgggggtcc gtccggctgc
     1201 ccggatgcat ccttaaggtc aaagagcgct gccgggtgtc ccatcgtctg caaagattct
     1261 cggagatcct cgaagggggc tatgaaagta tggaaggcag gtaccgcggg gttctgacat
     1321 gggcccttta ccatcgagcg aaggtgcttg tctttgccgg ggggacgttt gtggtcggtc
     1381 tttgcctcat gttcttcata ggaaaggagt tcgtgccgtc ggaggaccag agccgttttg
     1441 tcatccggct ccagacgccc atcgattatt cggtggatga agtggaccac atgaacatga
     1501 gggccgagga gatcgtcaag aagttccccg aggtccgaag cgtcctttac acgcagggcg
     1561 ggggacgaac gaaggaaacg aacaaggcga acctgtccgt caatctggtc ccgaaatcgg
     1621 agcgaggcaa gacccaggag gagatcaagg ccgagatgcg cagggccctg agaaagattc
     1681 cggggctcaa ggcctccgtg gagaacatat ccatgatagg ggcgggcaga ggcaggtggc
     1741 catcatgtac agcatccgcg ggctcgaatt ggacaggttg gaggcgtaca caagggagat
     1801 ccgggaccgg ttcggcaagt tgccggggat agtggacctc gacacgtccc ttgaaaaagg
     1861 caaaccggag gtccgcgttt tcatcgatcg cgacaaggct gccgatttgg ggatcgatgt
     1921 cgccacggtt gcggagaccg ccaatttcct tctgagcggc gaagtggaca tcaccaagtt
     1981 caaggacgag gccaggggca ggcgctatga cgtccgggtg aggctcaacc ccgagaacag
     2041 gagcgaccct gaggacattg gcgagatcta cgtcaggtcg aaggagggca aactggtcat
     2101 gctttccaac atcgtgagcc tgaaggaggg cggtggcccc agcgtcatca accgcgtcga
     2161 caggcagcgg gcggtgaccc ttttcgcgaa tctcgagann nnnnnnnngg ccagatcgga
     2221 actcgacgat atatcctcga aggtcctcac ctcggggtat tcaggcagct ataaagggca
     2281 ggccgacacg atgaaggaat ccttcggata tctcctgttc gccgcgatcc tgggtgtgat
     2341 catggcgtac atggtcctcg ccgcgcagtt cgaaagcttc gtgcatccct tcacggtcct
     2401 tctgtcgatg cccttctccc ttatcggcgc cttcggggcg ctcttcgtga cgggtaagac
     2461 cctgaatatc ttcagcatca tcggactgat ccttctcatg ggtctcgtga agaagaacgc
     2521 catccttctc gtggactaca cgaatatcct cagggagcgc ggcgccgtcc ggcgggacgc
     2581 gctgctcgag gcagggcccg tcaggctccg tcccatacta atgaccacct tcgccatgat
     2641 aatgggcatg atgcccatcg ccctcgggat cggtgagggc gccgaaacgc ggtcgcccat
     2701 ggctctcgcc accattggag gccttatcac ctcgctgctc ctcacgctcg tcgtcgttcc
     2761 cgtcgcctat gatctcttcg atgagatgaa ggaaaggtac tttccccgga aggacggtcg
     2821 ataatgaaga tgctcatcat cgtttacaac gacgtcgtcg acgaggaact tgtcgcgatg
     2881 ttaaagaagg caggcgtttc aggatacacc aagtggaagg aagctctggg tgaagggccg
     2941 gggacggggc ccaaactcgc cacgaacatc tggcccggca agaacaatgt tatcgctgtt
     3001 gttgtgaacg acgaggaact tcccgcggtt tcggggctcc ttcgggacct gaaggggtcc
     3061 catccgaaag ggggcataaa ggcctttatc cttgaggtcg agggaaccat ttagccccgc
     3121 ccctttaccg ggatggcccg tcgtgcttat cttatgaacc atgacctgtc tggatgaagg
     3181 tagagattgt aaggatatga aatgagcctg ttctttgtaa cgtttttcac gctctatacg
     3241 gcccttcacg cgtacgcctt cataaaagcg tggtacgcct ttcggttccg tccgctcacg
     3301 ggttgtatcc tcgccctctt tctcctgttc tgcgtcttca tgcctctctt tgtgcgtgcc
     3361 ctcgaacagg gagggcagga atccatcgcc cgcgtcttcg cctatgccgg ttacatctgg
     3421 atgagcgcca ttttcatctt ctttgccaca tcgctggtca tcgatatcgg caggttgtgc
     3481 gttatcatcg cgtcgtcgct gttcaaggtg gggc
//
LOCUS       DAOQTR010000206         3508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_30532_length_3508_cov_3.633073, whole genome shotgun sequence.
ACCESSION   DAOQTR010000206 DAOQTR010000000
VERSION     DAOQTR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30532_length_3508_cov_3.633073"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKN85_05935"
     CDS             complement(<1..446)
                     /locus_tag="PKN85_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HNT43980.1"
                     /translation="MESGVLSPINVSGLAAILSGFLSFFTPCILPLVPSYLIYISGIT
                     VGDVAAPTKAHRRRVFFHSLSFVLGFSFVFVALGVSTSLLGGFFAEHQTLIIRLGGII
                     LILLGLFYLDIIKISFFNRQHMMQLEQKPVGLAGSFVVGLTFSLGWT"
     gene            484..1401
                     /locus_tag="PKN85_05940"
     CDS             484..1401
                     /locus_tag="PKN85_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNT43981.1"
                     /translation="MKCAAIDIGTNTILLVVAERDDENEAFRDVVDMSTIVRLGEGLA
                     NSGRLSGPAMSRTMDGLRRYTDIAASHGVERLFCVGTAALREAANSGEFLASVKDAFG
                     FDVEVISARDEARYTYLSVRDDHTVRAPDMTIIDIGGGSTEVISGTDRDFGGYVSLPM
                     GSVRLTDAFVSHDPPIGEEMDRVVAYIREHLPAVTCRDGSTLVGTAGTVTNIAAMVAG
                     IGTYDKEIVHGFVIGLGQLKALIASLTGLTSDERRRVTGMEPGREDIIVQGAVILREI
                     MEHGGFDRVKVSAAGVRYGVIYERCGFAT"
     gene            1562..3061
                     /locus_tag="PKN85_05945"
     CDS             1562..3061
                     /locus_tag="PKN85_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43982.1"
                     /translation="MFQALIDNIPSPVYYKDARGVYLICNRAFREYFSVTDEQFIGKD
                     VFQLPLRRDEAEVHHAMDQELLAEPGVRSYELRSVRQDGTVRYDLNKKATLTADDGSV
                     GGIVGVIIDISEQKRMEEDILKAKNLQSLGTLAGGIAHDFNNLLMAIVGNLSLARMHT
                     PDNGRLMDYLNEAERIAFLGKALTQQLLTFSRGGNPIRSMVHPGILVRSVAERALRGF
                     PIDCVCDISPDLFPIEADEDQIRQVIENILRNARESMPAGGKITITARNVSISPEDRL
                     PLMTEDHVRISIADEGTGIAPEDIPKIFDPYYTTKGMGSEKGVGLGLAISYAIVRKHN
                     GSISIESTKGQGSVFHVNLPAYKHEAAQEAAAARRPAGTREGRVLLLDDEELVLEIGK
                     ELLEYLGYTVTTVQGGEEAVALYKQAMELKSPFDAVILDLAIPGSLGGKEVLRELLRI
                     DPGVKAIISSGYLNDPVVADYREYGFQDVLTKPYDSSELDEKLKKVIEG"
     gene            complement(3086..>3508)
                     /locus_tag="PKN85_05950"
     CDS             complement(3086..>3508)
                     /locus_tag="PKN85_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43983.1"
                     /translation="IPLPSGSGIAVLTPSGTVSPGNLNDASLVLKMTLGDRDFLFTGD
                     IGEDVEKALILSRAPLRSSVLKVPHHGSRFSSTTHFIRAVKPDLAVLSVGPGIRGIPS
                     PETVARYTALSIPLYRTDRDGCITVCTDGKKLTVEKEN"
BASE COUNT          798 a          914 c         1119 g          677 t
ORIGIN      
        1 gtccagccaa gggaaaaggt caggcctacg acgaaggagc ctgcgagtcc gacgggcttt
       61 tgctccagct gcatcatgtg ctgacggttg aagaaggata tctttataat gtcgaggtag
      121 aagaggccga ggagaatgag gatgatcccc ccgaggcgta tgatgagcgt ctgatgctcg
      181 gcaaagaagc cacccagcaa tgacgtggag acgcccaggg cgacgaagac gaaagagaat
      241 ccgaggacga aactcaggga atggaagaat acccgtctcc tgtgcgcctt cgtgggggcg
      301 gcgacatcac cgaccgttat cccgctgata tagatgaggt acgatgggac aagggggagg
      361 atgcagggcg tgaagaagga caggaacccg gacaggatgg cggcgagccc gctcacgttg
      421 atcggtgaca ggacgccgga ttccatgagt cgtatcttat gggttttctc gtgaggatgt
      481 caaatgaaat gtgccgcaat tgatatcggt acgaacacga tcctcctcgt cgtggcggaa
      541 agggacgatg agaacgaagc gtttcgcgat gttgtcgata tgtcgaccat cgtgaggctc
      601 ggggaggggc tggccaactc cggccgcctg agcggtcccg cgatgtcccg gacgatggac
      661 ggactcagac gttatacgga tatcgctgcg tcacatggtg ttgaacggct tttctgcgtg
      721 ggcaccgccg cgctgaggga ggccgcgaac agcggcgagt tcctcgcctc cgtgaaggac
      781 gccttcggtt tcgatgtcga ggtcataagc gcccgggacg aggcgcgcta cacctatcta
      841 tccgtgcgtg acgaccatac cgttcgggca ccggacatga ccataatcga tatcggggga
      901 gggagcacgg aggtgatcag cgggaccgac cgggattttg gaggatacgt ctcgctgccg
      961 atgggaagcg tcaggctcac cgacgccttc gtctcccacg accctcccat cggggaagaa
     1021 atggatcgtg tcgtcgcgta catacgggaa catcttcccg cggtgacctg tcgcgacggg
     1081 agcacccttg tcgggacggc aggcacggtg acgaacattg ccgccatggt ggccgggatc
     1141 gggacgtacg acaaggagat cgtgcatggc tttgtcatcg gcctcgggca gttgaaggca
     1201 ctgatagcat cgttgacggg attgacgagc gacgagagaa ggcgcgtcac gggcatggag
     1261 ccgggcagag aggatataat tgtccagggg gcggttattc tgagggagat catggaacac
     1321 ggcgggttcg acagggtcaa ggtcagtgcc gcaggagtca ggtatggcgt catatacgaa
     1381 aggtgcgggt ttgccacgta gtcggctgcc ttccgcccgc aggcctccgg acacccattt
     1441 ttttctatgg accgctgtgg tccttctgtg ataagaacca tataatgccg ttgaaagatt
     1501 cttttgaatc tgagagttta cactggaagc gctcgtagag atcctttcca aaacgcctca
     1561 catgtttcag gcgcttatcg acaacatacc cagtcctgtc tattacaagg atgcgagggg
     1621 ggtctacctt atttgcaaca gggcctttcg cgaatacttc agcgtcaccg acgaacagtt
     1681 catcggtaag gatgtcttcc agctgcccct gaggcgggac gaggctgagg tccatcacgc
     1741 gatggaccag gagctcctcg ccgagccggg ggtcaggtcc tacgagttga gatccgttcg
     1801 gcaggatggg acggtgcgtt atgacctcaa caagaaggcg acgctcacgg ccgatgacgg
     1861 aagtgtgggc gggattgtcg gcgtcatcat agatatcagc gaacaaaaga ggatggaaga
     1921 ggatattctg aaggcgaaga acctccagtc gctggggact ctggcgggtg gcatcgccca
     1981 tgatttcaac aatcttctca tggccatcgt gggcaacctg tcgctcgcca ggatgcacac
     2041 gcccgacaat ggacggctga tggactatct taacgaggcg gagaggatcg cttttctggg
     2101 aaaggccctg acgcagcaac ttttgacgtt ttcccgggga gggaatccca tccggagcat
     2161 ggtgcacccc ggcatccttg tgaggtctgt cgcggagcgg gcattgcgcg gttttcccat
     2221 cgattgcgtc tgcgacatat ctcccgacct ctttcccatc gaggctgatg aggaccagat
     2281 caggcaggtc atcgagaaca ttctcagaaa cgcgagagag tccatgccgg caggcggtaa
     2341 gatcaccata accgcgagga atgtgagtat ttcccccgag gacagacttc cgctgatgac
     2401 agaggaccat gtgaggatat ccattgccga cgaggggaca ggcatagcac ccgaggacat
     2461 tccgaagata ttcgacccct actacacgac gaagggcatg gggtcggaaa agggggtcgg
     2521 gcttggcctt gccatctcct acgcgatagt caggaaacac aacggcagta tttccatcga
     2581 gtcaacaaag ggacagggct cggtgtttca cgtaaatctc cccgcctaca agcatgaggc
     2641 ggcacaggag gcagcggcgg cacgcaggcc ggccggtaca cgtgagggca gggtccttct
     2701 tcttgacgat gaggaactcg tcctcgagat cgggaaggaa cttctcgagt accttggcta
     2761 cacggtgacc accgtgcaag gcggggagga agcggtggct ctctacaaac aggcgatgga
     2821 actgaaaagt cctttcgatg cggtcattct cgacctcgcc atcccgggaa gccttggggg
     2881 caaggaggtc ctgcgggagc tcctgcggat cgaccccggg gtcaaggcga tcatatcgag
     2941 cggatatctc aacgaccccg tcgtcgccga ctatcgggag tatggttttc aggatgtact
     3001 gacaaaaccc tacgattcga gcgagctcga cgaaaaactg aagaaagtca tcgaaggata
     3061 gataccgggg ttcggggaag gactttcagt tctctttctc cacagtcaat ttctttccat
     3121 ccgtacaaac agtgatgcag ccgtcgcgat cggtccgata aagagggatc gagagggcgg
     3181 tgtaccgggc gaccgtctcc ggagacggta tcccccggat gcccgggccc acgctcaaca
     3241 cggccagatc gggcttgaca gcgcggataa agtgggtcgt gctcgaaaag cggcttccgt
     3301 ggtgcgggac cttcaggaca gaggacctaa gcggtgcccg cgacaggatc agtgccttct
     3361 cgacatcttc cccgatgtcc ccggtgaaga gaaagtctct gtcgcccagt gtcatcttca
     3421 ggacgaggga cgcgtcatta agattgcccg gcgaaaccgt gccggaaggt gtaagcacgg
     3481 caatgcctga ccctgaggga aggggaat
//
LOCUS       DAOQTR010000207         3501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_30625_length_3501_cov_3.076030, whole genome shotgun sequence.
ACCESSION   DAOQTR010000207 DAOQTR010000000
VERSION     DAOQTR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30625_length_3501_cov_3.076030"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /gene="pdxA"
                     /locus_tag="PKN85_05955"
     CDS             <1..731
                     /gene="pdxA"
                     /locus_tag="PKN85_05955"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNT43984.1"
                     /translation="QPGISDTRCGEASYLYIREALKLVFTGEASAVVTCPISKASLHL
                     AGVPFPGHTELLAHSAGVDSYVMMLANPRIRVSLVTIHIPLKDVPARVTEEKVFRTIA
                     ITGRSLRESFSIDRPVIKVCGLNPHAGEQGVIGSEEDRISRAILRSREIGMDVTGPYP
                     GDSLFHHIDCDAYIAMYHDQGLIPVKTTDFRHTVNITLGLPFVRTSPGHGTGFDIAGK
                     GIADPASFIEAYRTAEIMTGTTRP"
     gene            997..2028
                     /locus_tag="PKN85_05960"
     CDS             997..2028
                     /locus_tag="PKN85_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT43985.1"
                     /translation="MTRRKAVSLLVAAVLIAAAVFLARTPADAASIVLKYANFPPATT
                     FPCIQMERWAKEVEKRTGGKVRVQTFPGGTLLPAKNIFDGVVAGMADIGNFAMSYQPG
                     RFPLSEAIDLPVGFKNARAASLALFDLVEKYQPKEFAKVKVITVFTCPPADIMTTMPA
                     RSLKELKGVELRTSGTGTDVLKRIGAIPIGMPQSEAPEAIQKGIVKGNVSSMEILKDF
                     NFAAYLPYATEANLFVVSFAVVMNKDRWDSLPADVKKVIDGLAREQAEWTGAYVDNHV
                     REAVEWSRKKYNHQVVRLSDADQNEITRLTKPMIDDYVKKVTSQGLPGKEIMKDVYAL
                     KQKYEKKYK"
     gene            2062..2565
                     /locus_tag="PKN85_05965"
     CDS             2062..2565
                     /locus_tag="PKN85_05965"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT43986.1"
                     /translation="MLMEDAMKYLERVNVALNGFLMVIGGFAVLALMTVATANVVLRM
                     VHAPYRGAYEVVSFLGAVVIAFALGYTEKKKGNIIVEILTERFPKMLQRTLEGASYVI
                     SGIFFSLVSWQIYEYGMKIMESGELSETLKIVFHPFVFAVSFGFAALALTLFVDLAGL
                     LSNREGK"
     gene            2567..3490
                     /locus_tag="PKN85_05970"
     CDS             2567..3490
                     /locus_tag="PKN85_05970"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT43987.1"
                     /translation="MEGPIVGVIGIVVMFAVLFLLRIPAAFTMAIVGFVGVVYVTSFK
                     AALVMIGADMWNIFSSYGLTVIPMFILVGEFAHWGGYNNSLYNATYRWFGHYRGGLAI
                     TTIMACAIFSAISGSNSATAATMSTVAIPEMKKYNYRPELNAGAVAAGATLGVLIPPS
                     IVLVIYGLYTGQSIGKLFFGNVIPSIILTVLIAATVFYICWRHPDWGPKGPRSTWRER
                     FGALPDLIEISLLFGIIMFALFTGCVTATEAAAASCALGLVMCVIRRKLSWKGFIASV
                     TDTLRISAMVFIIVAGAVIFGRFLAVTRLRR"
BASE COUNT          730 a         1077 c          986 g          708 t
ORIGIN      
        1 tacaacccgg catctcggac acccgttgcg gcgaagcgtc ctatctgtac atccgagaag
       61 ccttgaagct cgtcttcacc ggcgaggcgt cggccgtcgt tacctgcccc atcagcaagg
      121 cctccctgca tctcgcaggg gtccccttcc cgggccacac ggagttgctg gcccactcgg
      181 cgggagtgga cagctacgtc atgatgctcg caaaccccag gatacgggtg agcctcgtga
      241 ccatccacat ccccctcaag gacgtaccgg cccgggtaac ggaggaaaag gtctttcgga
      301 ccatcgccat caccgggcgt tcccttcggg aaagtttctc catagaccgc cccgtcatca
      361 aggtctgcgg cctcaatccc catgcaggcg agcagggcgt tatagggagc gaagaagacc
      421 gcatttcgcg ggccatactc cggtcccggg agataggcat ggacgtgacg ggaccctatc
      481 ccggcgactc gctcttccat catatcgact gtgacgcgta catcgcgatg tatcacgatc
      541 agggccttat acctgtgaag accaccgatt tcaggcacac cgtcaatatc acgctgggcc
      601 tgcccttcgt ccggacatca cccggccacg gcaccggctt cgacatagcc ggcaagggca
      661 tcgcggaccc cgctagcttc atcgaggcgt acaggacggc tgagatcatg acgggcacca
      721 cccgcccgta aaacgcatcc cggcaaggat tctcttgaca ccccccgcgc ctgtgtggta
      781 caaacaggcc atgtcaaaaa gggcagaaca cttcatcatg aacacaaaat gggcttggtg
      841 gtggcggaca tagcaggtgt gagaccgtaa atgttcggcc atggacggtt tcgcaccgtc
      901 catggccttc aagtttttaa ggccatgggt tctcgatacc ccatggcctt tttattttcg
      961 acatactgac atcaatacga ataaggagga tgttccatga caagacgtaa ggctgtttcc
     1021 ctgctggtcg cggcggtgct catcgccgcg gcggtatttc tggcgcgaac cccggcagac
     1081 gcggcgtcca tcgtcctcaa gtacgccaat ttcccgcctg ccaccacctt cccctgcatc
     1141 cagatggaaa gatgggcaaa ggaagttgaa aagaggaccg gcggcaaggt gagggttcag
     1201 acctttcccg gaggcaccct cctgccggcg aagaacatct ttgacggagt ggtggcgggt
     1261 atggccgata tcggtaattt cgcgatgagc taccagccgg gaaggttccc cctctccgaa
     1321 gcgatcgacc tccccgtggg cttcaagaac gcgcgcgcgg caagcctggc gctcttcgac
     1381 cttgttgaga aatatcaacc aaaagaattc gccaaagtga aggtcatcac cgtcttcacc
     1441 tgtcctcccg ctgacatcat gacgacgatg cccgcgcgtt ccctgaagga gttgaagggg
     1501 gtggagctcc ggacctctgg cacgggcacg gacgttctca agcggatcgg ggccataccc
     1561 atcggcatgc cccaatccga ggcccccgag gccatacaga aggggatcgt caagggcaat
     1621 gtctcctcga tggagatcct gaaagacttc aacttcgccg cgtaccttcc gtacgcgacg
     1681 gaggcaaacc ttttcgtcgt atccttcgcc gtcgtcatga ataaggacag gtgggattcc
     1741 cttcccgccg acgtgaagaa agtcatcgac ggccttgccc gggaacaggc ggaatggacc
     1801 ggggcatatg tggacaacca cgtgagagag gccgttgaat ggtccaggaa gaagtataac
     1861 caccaggtcg tccggctctc cgacgcggat cagaacgaga taacacgcct cacgaagccg
     1921 atgatcgacg attacgtcaa gaaggtcacc tcccaggggc tccccggcaa agagatcatg
     1981 aaggacgtat acgcgctgaa acagaagtac gaaaagaaat acaaatagag gggtcgtggg
     2041 ccagtacaca ggatccaatt catgctcatg gaggatgcga tgaagtatct cgaacgggtc
     2101 aatgttgctc tcaatggatt tcttatggtc ataggcggtt tcgccgtcct ggcgctcatg
     2161 acggtcgcga cggcgaatgt ggtgctcagg atggtccatg ccccctatcg gggggcctat
     2221 gaggtcgttt cgttcctcgg ggccgtggtc atagccttcg cgctcggata cacggagaag
     2281 aagaaaggca acataatcgt cgagatcctg acggagaggt tcccgaagat gctgcagagg
     2341 accctggaag gggcgagcta tgtcatcagc gggatcttct tttcccttgt ctcctggcag
     2401 atctatgaat acggcatgaa gatcatggaa tcaggcgagc tttcggagac cctgaagatc
     2461 gtcttccacc ccttcgtctt cgccgtctcc ttcggtttcg cagcacttgc cctgaccctc
     2521 tttgtcgacc tggcgggact cctctcgaac agggagggga aatagcatgg aaggcccaat
     2581 cgtcggagtt attggaatcg tcgtcatgtt cgcggtcctc ttcctcctca ggatccccgc
     2641 cgccttcact atggccattg tggggttcgt gggggtggtc tatgtcacct ccttcaaggc
     2701 cgcgctggtc atgatcgggg cagacatgtg gaacatcttc tccagctacg gtctgacggt
     2761 catccccatg ttcatcctcg tcggggaatt cgcgcattgg ggaggttaca acaacagcct
     2821 ctacaacgcc acctacaggt ggttcgggca ctatcgcggc ggacttgcga taaccaccat
     2881 catggcctgc gcgatctttt cggccatcag cggctccaac tccgccacgg cggccaccat
     2941 gagcaccgtc gccataccgg agatgaaaaa gtataactac cggcccgagc tcaacgcggg
     3001 cgcagtcgcc gccggggcga ccctgggagt cctcatcccg ccgagcatcg tcctcgttat
     3061 ctacgggctc tacaccgggc agtccatcgg caagctcttt ttcggtaacg tcatccccag
     3121 catcatcctg acggtcctta tcgccgccac cgtcttctac atctgctggc gtcaccccga
     3181 ctggggcccc aaaggtccga ggagcacatg gcgggaacgt ttcggggccc ttcccgacct
     3241 catcgagatc tcgctccttt tcgggatcat catgttcgcc ctcttcacgg gctgcgtcac
     3301 cgccacggag gccgccgccg ccagctgcgc cctgggcctc gtaatgtgcg tcatccgccg
     3361 taagctctcc tggaaggggt tcatcgcctc cgtcacagac accctgcgca tatcggccat
     3421 ggtcttcatc atcgtcgcag gcgcggtcat cttcgggagg ttcctggccg tcacgcggct
     3481 gcgacgatga tgaagaccat g
//
LOCUS       DAOQTR010000208         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_30640_length_3499_cov_3.702381, whole genome shotgun sequence.
ACCESSION   DAOQTR010000208 DAOQTR010000000
VERSION     DAOQTR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30640_length_3499_cov_3.702381"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PKN85_05975"
     CDS             complement(<1..599)
                     /locus_tag="PKN85_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNT43988.1"
                     /translation="MYHQGNRGRGKLFSLFFILAVCCLFLGASVLHPSARAEAASVTL
                     GWDAATGVSGYKIHRGTASGSYTTSIDVGNRTSYTLSDLTSGSTYYFAVTAYDTSSEQ
                     SGYSNEVSYAGGSSCTYSISPSSASFTSSGGTGTITVTAASGCTWSASTGVSWATISS
                     GTSGTGSGTVAYSVAANTGSSRTAAFSVAGQTFSISQAAA"
     gene            927..2378
                     /locus_tag="PKN85_05980"
     CDS             927..2378
                     /locus_tag="PKN85_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT43989.1"
                     /translation="MRLSIFSRLFLGYIAIILTLGAISAYTVLRLRQVNVEAGIIFNV
                     DQRVVDLKQKLVDSLLSHVGSEKKHVITGDPIFLEQMESGQKTFEGYLSQVSQIVGAS
                     SNRDILARVGSEYARYLSLVNEEIELSKTGRRYSRSEYGHRKEEAVGQVLEALKELEN
                     SYVNDIQERMNRIQETAGSAERLSGLMFAAAVLIVILGSLISTRNITRPLKVLVAKTR
                     EISRGVFEGDLNIGSPREVAELARAVNLMCEKLKKVDTMKSGFFATMSHELRTPLASI
                     KQGISLLRDGAGGPIPDKQKRLFAILSEETNRLIDLVNSLLELSKMEAGMMPFTFSKE
                     SIQSLIRRVVMEMTPLVETKKIRIEVDTGDENEIPPLKLDRERILQALRNLLGNAVKF
                     TPEKGRVTINARIKDRTVQFSMQDTGPGIPRENLETVFEKFHQSPGQAPGSIKGTGLG
                     LAFVKHIITAHGGKVWAESEPGQGTTFLFVLPL"
     gene            2405..2854
                     /locus_tag="PKN85_05985"
     CDS             2405..2854
                     /locus_tag="PKN85_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43990.1"
                     /translation="MPERAAEEPDRTSQAFSDARRDLDEAQILLARGNYQLSLMVLER
                     VLAGPAGRAHGDEVLFTMGLIFAHPGNQNRDYQKSAAFLRRLVKEYPSSSLRDQARTW
                     AEVLEENIKLRRASSEALQENQKLKQIIEQSKKVDIEIEEKKREEKR"
     gene            2864..>3499
                     /locus_tag="PKN85_05990"
     CDS             2864..>3499
                     /locus_tag="PKN85_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT43991.1"
                     /translation="MSAGKILVVDDERNLLELIQMGLEQAGYDVVTATDEVEALIQAR
                     EQVIDLAVLDLQLVRTDGITLMEQLHLISPRMPVIILTGYGSIESAVEAMKRGAYGYL
                     TKPFDSQVLLFEIGKMMENRRMSSEIERLQGLLESKYSFSNIIANSKKMQDVLETISR
                     IAKTDSTVLLLGESGTGKDLIAKAIHLASDRKEKSFVAINCAALPEPLLESN"
BASE COUNT          789 a          985 c         1051 g          674 t
ORIGIN      
        1 gccgccgcct gggaaatgga gaatgtctgt cccgccaccg aaaaggccgc ggtcctcgat
       61 gatcccgtgt tggccgccac cgagtaggcc acggtgccgc tccccgtacc gctcgtgccc
      121 gaggagatcg tggcccatga cactcccgtc gacgcgctcc aggtacaacc gctcgcggct
      181 gtcaccgtga tggtccccgt gccgccgctg ctggtgaagg aggcgcttga cggtgaaatg
      241 gaatacgtgc atgaactccc gcccgcatag ctcacctcgt tggagtaacc gctctgctcg
      301 ctcgatgtgt cataggcggt cacggcgaag tagtatgtgc tgccgctcgt aaggtcggaa
      361 agggtgtaac ttgtccggtt cccgacatcg atgctcgttg tgtagcttcc actcgctgtc
      421 cccctgtgta tcttgtatcc cgaaacgccc gtcgccgcgt cccagccgag tgtcacgctt
      481 gctgcctcgg ccctcgccga aggatggagc acggacgctc ccaggaaaag acagcacacg
      541 gcgagaatga aaaagagaga aaataacttg cctctacccc ggttgccctg gtgatacatt
      601 gaaacctcct gaacatttaa tacgtgccgg atcgactggc caattctcga tcctgccaca
      661 ggaatagcaa aagccgtgcc tcggggtgac gaaatgtgct atactatcga agttaaaggt
      721 aatgagatgt ttccgcagtt tgtctgtaag gcttcagatg cagggttttc ccttcatgtc
      781 ggatctctga ctgaaggata tttcctccac acactcctcc ccggtcaggc tgcatatcgt
      841 cccggcggat catcaggtac ctgatttaga ccggtcgccc gatccggtgc tgaacccggc
      901 acgggcccgg ctggaatgac tgtcccatga gactgagtat attttccagg ctgtttctcg
      961 ggtatatcgc cattattctt accctggggg ccatcagcgc gtacacggtc ctcaggcttc
     1021 gccaggtgaa cgtcgaggcg ggcatcatct tcaatgtcga ccagcgtgtt gtggacctca
     1081 aacagaaact cgtcgattcc ctgctttccc acgtggggtc ggaaaagaaa catgtcatca
     1141 ccggggaccc gatcttcctg gaacagatgg aatcggggca gaagaccttc gaagggtatc
     1201 tgtcccaggt gtcgcagatc gtaggcgcct cttcgaacag ggacatcctg gcccgggtgg
     1261 ggtcggagta cgcacgatac ctgtcgctgg tgaacgagga aatagaactg tcgaagaccg
     1321 gccggcggta cagcaggagt gaatacggac atcggaagga agaggccgtg gggcaggtgt
     1381 tggaggcgtt gaaggagctc gagaactcct acgtcaatga cattcaggag cggatgaaca
     1441 ggatacagga gacagcgggc tcggctgagc gcctgtcggg cctgatgttt gccgccgcgg
     1501 tgctgatcgt catcctgggt tccctcatca gcacgaggaa catcaccaga cccctgaagg
     1561 tcctggtggc gaagacaagg gagatctccc ggggcgtctt cgaaggggac ctcaatatcg
     1621 gctctccccg tgaggttgcg gaactggcca gggccgtcaa cctgatgtgc gagaagctca
     1681 agaaggtcga tactatgaag tccggcttct tcgccaccat gtcccatgaa ctgcgcactc
     1741 ctctcgcgtc catcaagcag ggtatcagtc tccttcggga cggtgcaggc ggaccgatac
     1801 cggacaagca gaaaagactg ttcgccatac tgtccgagga gacgaacagg ctcattgacc
     1861 tcgtgaactc ccttctggag ctatcgaaaa tggaggcggg catgatgccc ttcaccttca
     1921 gcaaggagag cattcagtcc ctgatccgtc gcgtggtcat ggagatgacg cccctcgtgg
     1981 agacgaagaa gatccgcatt gaggtggaca cgggtgacga gaatgagatc cctcccctca
     2041 agctggatcg ggagaggatc ctgcaagcct tgcggaatct cctcgggaac gcggtgaaat
     2101 tcaccccgga aaagggccgt gtaacgataa atgcccgtat caaggaccgt accgtgcagt
     2161 tctccatgca ggatacgggc ccgggcattc caagggagaa tctcgagacg gtcttcgaga
     2221 agttccatca gtcaccgggc caggccccgg gatcgataaa gggtaccgga ttgggcctgg
     2281 ctttcgtgaa acatatcatc acagcgcatg gaggtaaggt gtgggcggaa agcgaacccg
     2341 gacagggaac cacgtttctc tttgtattgc ccttgtgatg tttctcgcga ccttgggttg
     2401 cgcggtgccg gagagggcgg ccgaggagcc cgacaggacc tctcaggcgt tctccgacgc
     2461 gcgtcgggac ctcgatgagg cacagatact cctcgcgcgg ggcaactacc agttgtccct
     2521 gatggtcctg gagagggtcc tggccgggcc ggcgggccgt gcccatggag acgaagtgct
     2581 cttcaccatg ggcctcatct tcgcccatcc agggaaccag aacagggact atcagaagtc
     2641 cgccgccttc ctcaggcggc tcgtcaagga atacccctcc agttccctga gggaccaggc
     2701 aaggacctgg gcggaggtgc ttgaggagaa cataaagctc agacgtgcgt cctcggaggc
     2761 ccttcaggag aaccaaaaac tgaagcagat catagagcag tcgaagaagg tggatatcga
     2821 gatcgaggag aagaagaggg aagagaaaag ataggagaga cctatgtccg cgggtaagat
     2881 actggtagtc gatgacgaga ggaaccttct ggaactcatc caaatgggcc ttgaacaggc
     2941 gggctacgac gtggtgaccg cgacggatga ggtggaggct ctgatacagg cgagagagca
     3001 ggtgatcgat ctcgccgttc tcgaccttca actcgtgcgt accgacggca taacgctgat
     3061 ggagcaactc catctcataa gcccccggat gcccgtcatc atcctcaccg gttacgggag
     3121 catcgagagc gccgtggagg cgatgaaacg cggggcttac ggatatctca ccaaaccatt
     3181 cgattcccag gtccttctct tcgagatagg gaagatgatg gaaaaccggc ggatgtcctc
     3241 ggagatcgaa aggctccagg gccttctgga atcgaaatac agcttctcca acatcatcgc
     3301 caacagcaag aagatgcagg acgtgctcga gacgatatcg cgcatcgcga agacggactc
     3361 caccgtcctg ctcctggggg agagcggcac cggaaaggac ctcatcgcga aggcgatcca
     3421 tctcgccagc gacaggaagg aaaagtcttt cgtggccatc aactgcgccg cccttcccga
     3481 acccctcctg gagagcaac
//
LOCUS       DAOQTR010000209         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_31305_length_3446_cov_4.000590, whole genome shotgun sequence.
ACCESSION   DAOQTR010000209 DAOQTR010000000
VERSION     DAOQTR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31305_length_3446_cov_4.000590"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PKN85_05995"
     CDS             complement(<1..1395)
                     /locus_tag="PKN85_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNT43992.1"
                     /translation="MGVAKYTKDEKDRITDVTLQSGIHVKACYGPADLESVGFSYEKD
                     LAEPGQYPFTRGIHAEGFRSRAWTTRQYTGFGTPEESNERFKLMISHGQTGLNVAFDL
                     PTQMGYDSDDPMAEGEVGRVGMAIDSLRDFEIAFKGINLEKIGSGLTINAVASIMLAM
                     YQAVAEKYGFDPKKISATPQNDILKEMIGRGSWIFPVEPAVNLIGDTIEYSMNVLPRT
                     NPVSICGYHIRESGATPAQEIGYAILIANAYIDNVLKRGYDVDDFVGRFSFNLNVFGN
                     IWEQVAKFRAARKLWARNLKEKYGVKKDSNMYLRGLFGGGGYGLTKAQPENNIMRGAY
                     YALSAALAGAQTIALCSYDEAYTIPTPHSAIISLRTLQLLMDEIGLRDTVDPLAGSYF
                     IETITKEMEAKIEEEMGKIEKVGGIVQAVATGYVQRLVSQQAYAYEKGLQSGELKKVG
                     LNIHTEGEPMEVELH"
     gene            complement(1407..1808)
                     /locus_tag="PKN85_06000"
     CDS             complement(1407..1808)
                     /locus_tag="PKN85_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNT43993.1"
                     /translation="MDTRPIKVLVAKPGLDGHDRGAKVVAHALKEAGMEVIYTGLHRT
                     VDQIIRIAIQEDVDVIGLSIMSGAHIPITEKLMKKAREEGIDDKMVVVGGVIPGRDIP
                     KLRELGARGVFPGGTPFSEITSFINENVKKS"
     gene            complement(1846..2568)
                     /locus_tag="PKN85_06005"
     CDS             complement(1846..2568)
                     /locus_tag="PKN85_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT43994.1"
                     /translation="MKDTSKKTSAGAGPLKGIRALGKPRSMGDVAYDYLKQAIVKGDI
                     PPGQRLIENQLSAQMEVSRVPVREAVKKLEQEGLVERSGVRGFVVKGLNREEIEETLG
                     IRALLESYAAYLATEHITEAILKKLEDSIRAYRKAFEKKGNNTDKLMRLNTQFHEIIY
                     EAAGSGKLYSLINSFRDAIHRYRRPLLACEDYARVSLRDHEEMVKAMRKKDKKRVEAL
                     VRKHILRGMDIIIQELDAGKSV"
     gene            complement(2604..2879)
                     /locus_tag="PKN85_06010"
     CDS             complement(2604..2879)
                     /locus_tag="PKN85_06010"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT43995.1"
                     /translation="MKVEEKLALNAMKNDTMSHILLDQGICAACKERMCIAVCPAHLY
                     SLNEETGEMVVEYAGCLECGTCMIACIYGSITWDYPGGEFGVQYRYG"
     gene            complement(2882..>3446)
                     /locus_tag="PKN85_06015"
     CDS             complement(2882..>3446)
                     /locus_tag="PKN85_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT43996.1"
                     /translation="IHGAGGFDAPGLMEGFTGRPEVQRWIRGGELGEYSAHVISEAGI
                     GAIPKLYTGGMLVAGDAAGFALNLGLTVRGMEFAIASGVMAADTAVEALDANDLSENS
                     LSRYGKRLRESFVLPDMETFRHSREVLDRQRFFTVYPRFICELFDELFTVGDKPKEGL
                     YRTARRVAKKYVLNMETLKDLLAARKL"
BASE COUNT          606 a         1105 c          915 g          820 t
ORIGIN      
        1 gtggagctcc acttccatcg gttcgccctc ggtgtggatg ttgagcccca ccttcttcag
       61 ctccccggac tgcaatccct tttcatacgc gtaggcctgc tgtgatacaa gtcgctgcac
      121 gtagccggtg gcaaccgcct gcacgatacc gccgaccttc tcgatcttcc ccatctcctc
      181 ttctatcttc gcctccatct ccttcgtgat ggtctcgatg aaatagctgc ccgccagcgg
      241 gtctacggta tccctcaggc cgatctcgtc catgagaagc tggagggtgc gaagggatat
      301 gatcgccgaa tggggcgtcg ggatggtgta cgcctcgtcg tagctgcaca gggcgatcgt
      361 ctgggccccg gcgagagcgg cggaaagggc gtaataggcg ccgcgcatga tgttgttctc
      421 gggctgcgcc ttggtgagac cgtagccgcc tcctccgaag agacccctga ggtacatgtt
      481 cgagtccttc ttgaccccgt atttctcctt gaggttccgc gcccagagct tgcgcgccgc
      541 cctgaacttt gcgacctgct cccagatgtt gccgaagacg ttgaggttga aggaaaaacg
      601 gccgacgaaa tcgtcaacgt cgtacccccg tttcagaacg ttgtctatgt aggcgttggc
      661 gatgaggatg gcatagccta tctcctgggc gggcgtcgcc cccgactccc tgatgtggta
      721 accgcagatg ctcacggggt tcgtgcgggg cagaacgttc atggaatatt cgatggtatc
      781 accgatgagg ttcacggcgg gctcgacggg aaagatccag gagccccttc cgatcatctc
      841 tttcaggatg tcgttctgcg gcgtcgccga gatcttcttc gggtcgaaac cgtatttctc
      901 cgccacggcc tggtacatcg caagcatgat cgaggcgacg gcgttgatcg taagaccgga
      961 accgatcttt tcgaggttga tccccttgaa ggctatctcg aaatccctga gagaatcgat
     1021 ggccatgccg accctgccca cctcgccctc cgccatgggg tcgtcggaat cgtaccccat
     1081 ctgcgtggga aggtcgaagg cgacattgag gcccgtctgg ccatgggata tcatgagctt
     1141 gaaacgctcg ttcgattcct cgggtgtgcc gaacccggta tactgccgcg tcgtccacgc
     1201 cctgctgcgg aaaccctcgg catgtatccc ccgggtgaac gggtactgcc ccggctcggc
     1261 aaggtctttc tcgtaggaaa aacccacgct ctcgaggtct gcaggaccat agcacgcctt
     1321 gacatgtatc cccgactgca gggtcacgtc ggtgatcctg tccttctcat ccttggtata
     1381 tttcgccact cccattgcac gcctcctcag gacttcttca cgttttcatt gatgaagctc
     1441 gtgatctccg agaacggtgt cccaccgggg aacacccctc tggcccccag ctccctgagc
     1501 ttcgggatgt ccctgccggg tatgaccccc ccgacaacga ccatcttgtc gtcgatccct
     1561 tcctcacggg ccttcttcat caacttctcc gtgatgggaa tgtgagctcc gctcatgatg
     1621 gacagcccga tgacatccac atcctcctgg atggctatcc tgatgatctg atccaccgtc
     1681 cggtgaagcc cggtgtagat cacctccatg ccggcctcct tgagcgcgtg ggcaaccacc
     1741 tttgccccgc ggtcgtgtcc gtccagtccc ggcttcgcaa ctaagacttt gatgggcctc
     1801 gtgtccaatt gtacctcctt gcttgtttct tccgctcttt cgccgctaca ccgacttgcc
     1861 cgcgtcgagc tcctgaatta tgatatccat cccccgcagg atgtgcttcc tgaccagcgc
     1921 ctcaaccctc ttcttgtctt tcttcctcat cgccttgacc atctcctcgt ggtcacggag
     1981 cgataccctc gcgtaatcct cgcaggcgag aagaggtctc cggtaacgat gaatggcatc
     2041 cctgaaggaa tttatcaggg aatagagctt gccgcttccc gcggcctcat atatgatctc
     2101 gtggaactgc gtgttgagcc gcatgagctt gtccgtgttg ttgcccttct tctcgaaggc
     2161 cttcctgtaa gcgcgaatgc tgtcctcgag cttcttcaat atcgcctcgg tgatgtgctc
     2221 cgtggcaagg tatgctgcat agctttcgag aagggccctg atgccgagcg tctcttctat
     2281 ctcctcccgg ttgaggcctt tcacgacgaa cccccggacc ccgctgcgtt ccacgaggcc
     2341 ttcctgttcc agtttcttca ccgcctcccg cacgggaacc cggctcacct ccatctgcgc
     2401 cgaaagctgg ttctcgatca ggcgctgtcc cggggggata tctcccttga cgatggcctg
     2461 cttgagataa tcataggcca cgtcccccat tgaacggggt ttgcccagag ccctgatacc
     2521 cttcaggggg cctgctcctg ccgatgtctt ttttgacgtg tctttcattt cgtctccatt
     2581 cagtccgcgc ctctggtgtc tcatcaaccg tatctgtact gcacgccaaa ttcacccccc
     2641 ggataatccc aggttatcga cccgtagata caggctatca tgcaggtgcc gcactccagg
     2701 catccggcat actcgacgac catctctccc gtttcctcgt tgagggaata caggtgtgcc
     2761 gggcagacag cgatgcacat gcgctccttg caggccgcgc aaatgccctg gtcgagaagg
     2821 atatggctca tagtgtcatt cttcatcgcg ttcaaggcga gtttttcttc caccttcatt
     2881 gtcatagctt cctcgcagca agaaggtcct tcagggtttc catattgagg acatatttct
     2941 tcgcaaccct cctggcagtc ctgtaaagac cttccttcgg tttgtctccc acggtgaaaa
     3001 gctcgtcgaa gagctcgcag atgaacctgg ggtagacggt aaagaacctc tgcctgtcaa
     3061 ggacctcccg ggaatgacgg aaggtctcca tgtcgggcag cacgaaactt tctctcagtc
     3121 ttttcccgta gcgcgacagg gaattctctg agaggtcgtt tgcatccagg gcctcgacgg
     3181 cggtgtcggc cgccatcacg cccgaggcga tggcgaattc catgccccgt accgtgagac
     3241 ccaggttgag ggcaaagcct gccgcgtcgc ccgcgaccag catgccgcct gtgtacagct
     3301 tcgggatcgc gcctattccc gcctcggaga tgacgtgtgc cgaatactct ccgagttcgc
     3361 ctccccggat ccagcgctgg acctcgggac gcccggtgaa cccctccata agccctggag
     3421 cgtcgaagcc gcccgcgccg tgtatg
//
LOCUS       DAOQTR010000210         3431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_31491_length_3431_cov_3.211197, whole genome shotgun sequence.
ACCESSION   DAOQTR010000210 DAOQTR010000000
VERSION     DAOQTR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31491_length_3431_cov_3.211197"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKN85_06020"
     CDS             complement(<1..667)
                     /locus_tag="PKN85_06020"
                     /inference="COORDINATES: protein motif:HMM:NF025128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SwmB domain-containing protein"
                     /protein_id="HNT43997.1"
                     /translation="MKDPEGAVVHEGRVDLELPLTTAEGVLELPPLTHVFDRCGQYPV
                     EVSVRSGEVLCGQVRTTIRVAAATRIDVVKTVDPATVTPDGDKRVRVAIRLEGVGATV
                     NPSFLSAGTNTGGDRITIAFDKAMAEPGGGVPLFTVTADGAPCPVTEVLLGESDITRI
                     DLVLAAAVARGQEILVSYAGSSLTCVEGKPLMPFDDEAVANRVSPPIFNQDGYGFSGS
                     IAPN"
     gene            complement(761..1897)
                     /locus_tag="PKN85_06025"
     CDS             complement(761..1897)
                     /locus_tag="PKN85_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HNT43998.1"
                     /translation="MTNVHDKEALDGDAIVGSRGVSGDGAADMAFRYLLSLQRNDGGW
                     GASQDGPSSIHMTAVAVASLRRGPAGEDENGRVALEKATEYFLARQHPDGGFGDAFST
                     VHETALACHALEGLLDDPNVVLRAKQYLIQTQSDNGSWNNDLYSTALALEACLASGED
                     ITGAGRGEEETGAGEGLRAGAEPGDAEDGSPGSVGIPSARLDRTGTEGGGDASASPKV
                     QSRATNERTKISLVSRRRASAAPVPDAAQETASTGGSVVVRSVDTDRNEYGPNETVRI
                     CSTIENPSEADFSIVVNAQLADARGHIVDVAAHETGPAVKLGAGASESVTLLWNTGMS
                     QPGAYSVRFHVADAASGSVVDDRKLAFVVTPSVVVDDLDLVVSP"
     gene            complement(2264..2707)
                     /gene="dtd"
                     /locus_tag="PKN85_06030"
     CDS             complement(2264..2707)
                     /gene="dtd"
                     /locus_tag="PKN85_06030"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT43999.1"
                     /translation="MRAVIQRVKSASVEVDGRCVGQTGPGLLVFLGIAKGDGEKDIDW
                     LIEKIVNLRIFEKEDGKFDESLLDRGGSLLVVSQFTLYGDCSRGRRPSFSDAMPSDEA
                     KVFFESFVERARLRVPDVQTGVFQAHMEVTLRNDGPVTLIIESRK"
     gene            complement(2822..>3431)
                     /gene="fusA"
                     /locus_tag="PKN85_06035"
     CDS             complement(2822..>3431)
                     /gene="fusA"
                     /locus_tag="PKN85_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT44000.1"
                     /translation="KQSGGKGQYGDTWLELGPKARGEGFEFVDKIVGGAIPRQYIPAV
                     EKGIVEAMNQGILAGYPVVDFRVTLYDGSYHDVDSSEMAFKIAASMGFKKGLEMCKPT
                     LLEPIMKMEVIVPDENMGDIMGDMNSRRGKIMGVETKGSNQVVKAVVPMAEVLRYAPD
                     LRSMTGGRGTFTMDFSHYEEVPAQIADKIVEAAKREKEAQEK"
BASE COUNT          646 a         1158 c          926 g          701 t
ORIGIN      
        1 ggttcggcgc gatggagccg ctgaaaccgt atccgtcctg gttgaatatg ggcggcgaca
       61 ccctgttcgc gaccgcttca tcgtcaaaag gcatgagggg cttgccttcc acgcacgtga
      121 ggctcgagcc cgcatagctc acaaggatct cctgcccccg tgccaccgcc gcggcgagaa
      181 caaggtcgat cctggtaatg tccgattccc cgagaagcac ctccgtgacg ggacagggcg
      241 ctccatcggc ggtgaccgtg aacaatggga ccccaccgcc cggctccgcc atggccttat
      301 cgaaggcgat agttatcctg tcgccgcccg tattcgtgcc ggcggacagg aaggaggggt
      361 tgaccgtcgc accgacacct tccagccgga tggccacccg cacccgcttg tccccgtccg
      421 gtgtgaccgt cgcgggatcc acggtcttca cgacgtcgat cctcgtcgcc gcggcgaccc
      481 gtatggtggt cctcacctgt ccgcatagga cctctcccga acggaccgac acctccaccg
      541 ggtattgccc gcagcggtca aagacatggg tcaggggagg cagctccaga accccctcgg
      601 ccgtcgtcaa cggcagttca agatcgaccc gtccttcatg gaccaccgct ccctccgggt
      661 ccttcaccag cagctccgcg gtcaaaaatg cgtccgtatt ggacctgttc tgcaacgtga
      721 gacacatgtc caccgttctc ttttcatcct ggttgagatt ctaaggggac acgaccagat
      781 cgagatcgtc gacgacgaca gaaggggtga cgacaaatgc tagcttcctg tcgtcgacga
      841 cgctcccgct ggcagcgtca gcgacatgga aacgcacact gtaggcgccg ggctggctca
      901 tgccggtgtt ccacaggagc gtcacggact cggaggcccc tgcgccgagt ttcaccgcgg
      961 gaccggtctc gtgggccgcc acatccacta tatggccgcg ggcatccgcc agctgagcgt
     1021 tgacaacgat gctgaagtcc gcctccgacg ggttctcgat ggtggagcat atgcgtaccg
     1081 tttcgttcgg gccatattcg ttcctgtccg tgtcgacgga acgcacgaca acgctcccgc
     1141 ccgtcgaggc agtctcttga gcagcatccg ggacgggagc ggcactggcc cttcggcgcg
     1201 aaacgagaga gatctttgtc cgttcgttcg ttgccctgga ctggaccttc ggactcgcac
     1261 tcgcatcgcc cccaccttcc gttcccgtcc tgtccagtct ggccgatggt attccgacag
     1321 acccgggaga cccatcctcc gcatcacccg gctctgcgcc cgcgcgcaac ccttccccgg
     1381 ctcccgtttc ttcctccccc ctcccggccc ctgtaatatc ttcgccggaa gcaaggcagg
     1441 cctcgagagc aagcgcggtc gaatacaggt cgttgttcca cgaaccattg tcggattgcg
     1501 tctgaatgag gtattgctta gccctgagca cgacgttcgg gtcgtccagc agaccctcga
     1561 gggcatgaca tgccagggcc gtttcgtgta cggtggagaa ggcgtctcca aaacccccat
     1621 cggggtgctg cctcgccaaa aaatattccg tcgccttttc cagtgcaacg cggccgttct
     1681 cgtcctctcc cgcaggcccg cgccttagcg acgccacggc cacggcggtc atatgaatgg
     1741 aactcggccc gtcctgcgac gccccccacc cgccgtcgtt cctctggagg gaaagaaggt
     1801 aacgaaatgc catgtctgcc gcaccatcgc ccgaaacacc acggcttccg acgatagcgt
     1861 ccccgtcaag ggcctctttg tcatgaacat tcgtcatagc caccggacct ccccgagcgg
     1921 aacccgcatg gacggccttg atacacgcct tccaggacct tttcgggaaa caggcacggc
     1981 ccgcattcgt tgctcacgtc ttggtatcat ccagcttcgc atccacagcc acgcaaagtg
     2041 tcgccgccca tccgcagcgc atagcagtaa ccctggggga accgatgatg attccactcg
     2101 cgatccgtcg accgtccccg gtacaggtgt aatgcaacgt atatattgaa gatgaaatgt
     2161 gattatacta gcggtatgag taccttgtca agacaaatgt gatgagatgt catacctagg
     2221 cctgccctat gttataccgg gtcacgcgac aaccccgtcg gggctatttt cgggattcta
     2281 tgatcagtgt taccggaccg tcattcctga gggttacctc catgtgggcc tggaacaccc
     2341 cggtctgcac gtcgggaacg cggagcctcg ccctctccac gaagctctcg aaaaagacct
     2401 tcgcctcgtc cgacggcatg gcgtcggaaa aggaagggcg gcgtcctctg ctgcagtcgc
     2461 catacagggt gaactgggaa acaacaagaa gcgaaccccc tctgtcgagc agagattcat
     2521 cgaacttgcc gtcctccttc tcgaagatcc tcaggttcac gatcttctcg atcagccagt
     2581 cgatatcctt ctcgccgtca cccttggcga taccgagaaa gacgaggagg ccgggtcctg
     2641 tctgcccgac acaccgtcca tcaacctcaa cggaagcact cttgactcgc tggataacgg
     2701 cccgcatctg tcctccttat caggttcctg cgccccctca ggctttccgg aatgggaaca
     2761 gcaaaagggc cccttttcca ggggcccatc tcataccatt atgtatccgc gcacggtggc
     2821 tctatttctc ctgcgcctct ttctcgcgct ttgcagcctc gactatcttg tccgcgatct
     2881 gtgcgggaac ttcctcgtag tgggagaaat ccatggtgaa tgttccccgc ccaccggtca
     2941 ttgacctcag gtcgggcgcg tacctcagca cctcggccat gggcaccacc gccttgacca
     3001 cctggttgct ccccttggtc tccacgccca tgatcttgcc cctacgggag ttcatgtcgc
     3061 ccatgatatc ccccatgttc tcgtcaggca cgatcacttc catcttcatg atgggctcga
     3121 ggagagtcgg cttgcacatc tcgagaccct tcttgaaacc catcgacgcc gctatcttga
     3181 atgccatttc cgacgagtcg acgtcgtgat aggagccgtc ataaagggtg accctgaaat
     3241 cgacgacggg atagcccgcc aggataccct ggttcatggc ctccacgatg cccttttcga
     3301 cggcgggaat gtactgcctg ggaatggccc cgcccacgat cttgtccacg aattcgaaac
     3361 cctcgccgcg ggccttggga ccgagctcca gccacgtgtc gccgtactgg cccttgccgc
     3421 cggactgttt c
//
LOCUS       DAOQTR010000211         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_31558_length_3426_cov_3.359537, whole genome shotgun sequence.
ACCESSION   DAOQTR010000211 DAOQTR010000000
VERSION     DAOQTR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31558_length_3426_cov_3.359537"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /gene="pyrH"
                     /locus_tag="PKN85_06040"
     CDS             <1..608
                     /gene="pyrH"
                     /locus_tag="PKN85_06040"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNT44001.1"
                     /translation="IGDVRGLGVEVAIVLGGGNIYRGKKGEKEGMDRVTGDYIGMVAT
                     VINALVLKDTLVRMGTPAVVQTALALERIAEPYDHARARGYLDEGKIVIFACGTGNPY
                     FTTDTAAALRAVEMKADALMKATKVDGVYDRDPEIHADAVFLPEISYSEVLERDLQVM
                     DLTAITLCKENSIPIAVFSIRVRDNLKKVVLGEKVGTIVRR"
     gene            612..1169
                     /gene="frr"
                     /locus_tag="PKN85_06045"
     CDS             612..1169
                     /gene="frr"
                     /locus_tag="PKN85_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNT44002.1"
                     /translation="MSNDVMTELEDKLKKSLAALNKDFSKLRTGVASVTLLEDIKIDY
                     YKQPTPLNQVATIGVPDSRTITIQPWDNTVIAEIEKAIQKSDLGVNPMSDGKTIRLTF
                     PKLTEERRKELTKQGSKMLEGTRVAMRNVRRDINEKLKKLEKDKAISQDDLKKKQDDV
                     QKLTDKHIDMAEKLFSEKEKEILSI"
     gene            1182..1901
                     /gene="uppS"
                     /locus_tag="PKN85_06050"
     CDS             1182..1901
                     /gene="uppS"
                     /locus_tag="PKN85_06050"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNT44003.1"
                     /translation="MTKTAPDRLPTHVAIIMDGNGRWAKQQKLRRAEGHKVGIDSVRV
                     ITETTCDLGIPYLTLYAFSKENWSRPKEEVKMLMFLLGLYLEQELPMMMDRGIRFNII
                     GERGDFSKSLQTQFDDVMRKTARNKNLVLTIALSYSGRKEIMRSVRLLCEDFRKGLVK
                     RIDERAFRRHLYSPAIPDPDFLIRTSGEMRLSNFLLWQVAYTEIYVTDILWPDFRKQA
                     FLDALAEFAKRDRRFGNVKDF"
     gene            1902..2702
                     /locus_tag="PKN85_06055"
     CDS             1902..2702
                     /locus_tag="PKN85_06055"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNT44004.1"
                     /translation="MGELKKRIITGVCVAPVIAVLFYILPPVWLLALLLAIAVVAVLE
                     AASLAGCRMKYLIAVLVIAGVVPLYFRFFQIYMLWMMASAFIIITIKVFDRRASSEDA
                     NRDLTGQTAVVLFCNFFILIPFFYLYLLKELQALFPLILLFSIWASDIFAYGIGKKFG
                     KHPLAPRISPKKTVEGLMGATLGSLIVITASHQLLGLSVPAALGVGAATGVLGQAGDL
                     LESACKRVFNTKDSSQLIPGHGGILDRIDSFIFTAPFLYTCLVWTRYL"
     gene            2699..>3426
                     /locus_tag="PKN85_06060"
     CDS             2699..>3426
                     /locus_tag="PKN85_06060"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNT44005.1"
                     /translation="MKKKVLILGSTGSIGTATLDVIANDPGAFEVRGLACRNNIELLN
                     RQIARFKPQAVCVYDQRQRSAVKAPGVRVHTGIEGMKEMIRSDVDTVVNALPGSIGLE
                     PTIEALRCGKVLALANKESLVMAGRIIRRILSEKGSRLIPVDSEHSALYQLLKGVEGK
                     EVRSLIITASGGPFRRHTKRALESVELSEAMNHPTWRMGQKITLDSATLMNKGLEIME
                     ARWLFDLEPGKIKTLVHPESIVHG"
BASE COUNT          810 a         1025 c          948 g          643 t
ORIGIN      
        1 agataggaga cgtccgcggc cttggcgtcg aggtcgccat cgttctcgga ggcggcaaca
       61 tataccgcgg taagaagggc gagaaggagg ggatggaccg cgtcaccggg gactacatcg
      121 gtatggtggc cacggtcatc aacgcccttg tccttaagga caccctggtc aggatgggaa
      181 ctccggcggt ggtgcagacg gcgcttgccc tggaaaggat cgccgaaccc tatgaccatg
      241 cgcgggccag aggctacctc gacgagggaa agatagtcat cttcgcctgc ggcaccggca
      301 acccctattt caccaccgac acggccgcgg cccttcgcgc tgtcgagatg aaggccgacg
      361 cgctcatgaa ggccacgaag gtggacggtg tctacgacag ggaccccgag atccatgccg
      421 acgccgtctt cctcccggag atatcctaca gcgaggtcct ggagagagac cttcaggtca
      481 tggacctcac cgctataacg ctttgcaagg agaacagcat ccccatcgcc gtcttcagca
      541 tacgcgtcag ggacaacctg aaaaaagtgg tccttggcga aaaagtaggg acaatcgtga
      601 ggaggtaacc tatgagcaat gacgttatga ccgaactgga ggacaagcta aagaaatcgc
      661 ttgcagcgct gaacaaggac ttctcgaagc tgcgcaccgg cgtcgcttcg gtaaccctcc
      721 tggaagatat caagatagac tattacaagc agcccacacc gttgaaccag gtggcgacca
      781 tcggcgtccc cgattcacgt accatcacca tccaaccatg ggacaacacc gtcatcgcgg
      841 agatcgaaaa ggcgatccag aaatcggatc tcggcgtcaa tcccatgtcc gacggcaaga
      901 ccatccggct gaccttcccg aagcttaccg aggagaggcg caaggaactc acgaagcagg
      961 gcagcaagat gctcgagggt acccgcgtcg ccatgagaaa cgtccggagg gacataaacg
     1021 agaagctgaa aaagctggaa aaggacaagg ccatttccca ggacgatctc aagaaaaagc
     1081 aggatgacgt ccagaagctc acggacaaac acatagacat ggcagaaaag ctcttttccg
     1141 agaaagagaa agagatcctc tccatctgag tgacgctact gatgacaaag acggctcccg
     1201 acagattgcc cactcacgtc gccataatca tggacgggaa cggcaggtgg gcgaaacaac
     1261 agaaactcag gagagcggaa ggccacaagg tcgggatcga ctcggtccgt gtgatcacgg
     1321 agacgacctg cgacctcggc ataccctatc tcaccctcta cgccttttcc aaggaaaact
     1381 ggtcccgccc gaaggaagag gtcaagatgc tcatgttcct tctcggcctc tacctcgaac
     1441 aggaactccc catgatgatg gacagaggca tacgcttcaa catcatcggc gagaggggtg
     1501 atttctccaa gtctctccag acacagttcg atgacgtaat gaggaagacg gcacgcaaca
     1561 agaacctcgt cctgaccata gccctcagct acagcggacg caaggagatc atgcgctccg
     1621 tgcgcctcct ctgcgaggat ttcagaaagg gcctcgtgaa gaggatcgac gaaagggcct
     1681 tcaggaggca tctttacagc ccggcgatac ccgatcccga ctttctgatc cgcacgagcg
     1741 gcgagatgag actctcgaac ttcctcctct ggcaggtggc ctacaccgag atctacgtga
     1801 ccgatatcct ctggcccgac tttcgaaaac aggcctttct cgacgcgctg gcagaattcg
     1861 cgaagcgtga cagaaggttt ggaaacgtga aggatttctg attgggagaa ctgaaaaagc
     1921 gcatcatcac gggcgtatgc gttgcccccg tcattgccgt gctcttctat atcctgcccc
     1981 cggtgtggct cctcgccctt cttctcgcca tcgccgtcgt ggcggtcctc gaggcggctt
     2041 cccttgccgg ctgccgcatg aagtacctta ttgccgtcct cgtcatcgcc ggggtcgtgc
     2101 ccctttattt ccgtttcttc cagatataca tgctctggat gatggcatcg gcattcatca
     2161 tcatcaccat caaggtcttc gacagaaggg cgtcgtccga ggacgcgaac cgtgacctca
     2221 cgggccagac cgctgttgtc ctcttctgca acttcttcat cctcatcccc ttcttttatc
     2281 tctatctcct caaggaattg caggcgctgt ttccccttat ccttctcttt tccatatggg
     2341 caagcgacat cttcgcctac ggcatcggga agaagttcgg gaagcacccc ctggcccccc
     2401 ggatcagtcc gaagaagacg gttgaaggcc tcatgggtgc aaccctgggc tctctgatcg
     2461 tcatcaccgc gtcccaccaa ctgcttggtc tcagcgtccc cgccgccctc ggtgtgggag
     2521 cggcgacggg cgttctcgga caggcgggcg acctcctcga atccgcatgc aagagggtat
     2581 tcaacaccaa ggattcatcg cagctcattc ccggacacgg cggtattctg gaccgtatcg
     2641 acagcttcat cttcacggcc cccttcctat acacctgtct ggtatggacg agatacctat
     2701 gaagaagaaa gtcctcatat tgggttccac cggctccatc ggcaccgcga ccctcgatgt
     2761 cattgcaaac gaccccgggg ccttcgaggt ccgcggtcta gcctgcagga acaacataga
     2821 actgctgaac cggcagatag cccggtttaa gccccaagcg gtctgcgtct acgaccaaag
     2881 gcagagaagc gcggtcaagg cgcccggcgt acgcgtccac acgggcatcg aaggcatgaa
     2941 ggaaatgatc cgctccgacg ttgacaccgt cgtcaatgcc ctgcccggga gcatcgggct
     3001 cgaacctacc atagaggccc tgcgctgcgg gaaggtcctt gccctcgcca acaaggaaag
     3061 ccttgtcatg gcggggagga tcatcagaag gatactgtcc gaaaaagggt cgcggctcat
     3121 acccgtcgac agcgagcact ccgctctcta tcagctcctc aagggtgtgg agggcaagga
     3181 ggtccggtcc ctcatcataa ccgcctcggg ggggcccttc aggagacaca cgaaaagggc
     3241 cctggaaagt gtggagcttt cggaagccat gaaccatccc acgtggagga tggggcagaa
     3301 gatcacgctt gattcggcta cgctcatgaa caaagggctg gagatcatgg aggcgcgctg
     3361 gctcttcgat ctcgagccgg ggaagataaa gacccttgtc catcccgaaa gcatcgttca
     3421 cggcat
//
LOCUS       DAOQTR010000212         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_31795_length_3408_cov_3.567551, whole genome shotgun sequence.
ACCESSION   DAOQTR010000212 DAOQTR010000000
VERSION     DAOQTR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31795_length_3408_cov_3.567551"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PKN85_06065"
     CDS             <1..907
                     /locus_tag="PKN85_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44006.1"
                     /translation="VIIMTDADVDGSHIRTLLLTFFYRQMPDLITDGHLFIAQPPLYK
                     IKSGGKESYAKDEDEFERAITQRGMEKTRCAVNGDTQEGPELAEKVEKIKTVEGFLRN
                     SLVDGIDREISLGLMRFDIVKREDFQKEDKLGRLLESLRGSGYRCTMVWDREHNLFCI
                     EAEAKGSKGRMIRVEYEMCAVSDYADAYKAYVEVRNFYEEGVRVTDGNKPPVSFSPDE
                     FLRHILEKGKEGVNIQRYKGLGEMNAEQLWETTMNPEKRRVVKVTIEDAVAADQVFTV
                     LMGNNIETRRTFIEENALSVRNLDI"
     gene            904..1521
                     /gene="coaE"
                     /locus_tag="PKN85_06070"
     CDS             904..1521
                     /gene="coaE"
                     /locus_tag="PKN85_06070"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNT44007.1"
                     /translation="MITIGITGIIGSGKTTASAALKARGIPVIDLDALAKKMLTLAEV
                     HEEIEREFGDGLVIDGQIVVEKLRDIVFADREKLRRLERITHPRVRRELWREVGERAE
                     AGEKAIVVDGPLLFETGLYKELDRTVVVTAGMDVIRERLKLRGMAPDDIERRISNQIP
                     LKEKEEAADYVLLNNGTREDLERNIEALLERINEWEVRTRCTSTT"
     gene            1503..2756
                     /gene="rho"
                     /locus_tag="PKN85_06075"
     CDS             1503..2756
                     /gene="rho"
                     /locus_tag="PKN85_06075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT44008.1"
                     /translation="MHLNDLKSKKIGELTQIARDLNIENASGLRKQELIFSILQAYVD
                     KNENVYGEGVLEILSEGFGFLRSTDSNYLPGPDDIYVSPSQIKKFGLRTGDTTSGQIR
                     PPKDNEKYFALLKVESINFDDPSVVRDKIIFDNLTPIYPNERAKLETVHDNLSTRVMD
                     LFTPIGKGQRGLIVAPPRTGKTMLLQSIANSLTKNHKEIYLIVLLIDERPEEVTDMMR
                     SVKGEVISSTFDEPATRHVQVAEIVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVP
                     SSGKILSGGIDASAMQKPRRFFGAAKNIEEGGSLTIIATALIDTGSRMDEVIFEEFKG
                     TGNCEIYLDRKLAEKRIFPAIDINKSGTRKEELLLGSSDLQRIWLLRKVLQPMNTVEA
                     MEFLLEKLADTDSNKDFLYSMSKGG"
     gene            2762..2968
                     /gene="rpmE"
                     /locus_tag="PKN85_06080"
     CDS             2762..2968
                     /gene="rpmE"
                     /locus_tag="PKN85_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNT44009.1"
                     /translation="MKKGIHPELRTAMVKCACGHTFETLSVKDKITVEICAKCHPIFT
                     GKEKRLDSAGQVEKFERKYGKKQK"
     gene            2968..>3408
                     /locus_tag="PKN85_06085"
     CDS             2968..>3408
                     /locus_tag="PKN85_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNT44010.1"
                     /translation="MFGKLIELEKRFDEIEAEMVKPEVLSNMEEYKKLAKERTDLKEV
                     VDPYREWKAAKAEHEKTSELLKQESDEDMKTLAREELASLQKEMERIEGILTDSLLRK
                     TEKQAKSMFLEIRAGTGGEEAALFARDLFASYMKFAERMRWKTEI"
BASE COUNT          887 a          913 c         1052 g          556 t
ORIGIN      
        1 ggtgatcatc atgaccgacg ccgacgtcga cggttcccat atacgcacgc tcctgctcac
       61 cttcttctac cgccagatgc ccgatctcat cacggacggg catcttttca tcgcccagcc
      121 gcccctgtac aagataaagt ccggcggtaa ggaatcctac gcgaaggacg aagatgaatt
      181 cgagcgcgcc atcacgcaga ggggcatgga aaagacccgc tgtgccgtta acggcgacac
      241 gcaggaggga ccggagctcg ccgaaaaggt ggaaaagata aagacggtcg agggctttct
      301 gcgcaattcc ctcgtggacg ggattgacag ggagatatcc ctcggtctca tgcgcttcga
      361 tatagtgaaa cgggaggact ttcagaagga ggacaagctc ggcaggctgc tcgagagctt
      421 gaggggttcg ggctacaggt gcacgatggt ctgggaccgg gagcacaacc tcttctgcat
      481 cgaggcggag gcgaagggca gcaaaggaag aatgatccgt gtggagtacg agatgtgtgc
      541 cgtgagcgac tatgccgacg catacaaggc gtacgtggag gtgaggaact tctacgagga
      601 aggcgtgcgc gtcaccgacg ggaacaagcc tcccgtctcc ttcagccccg acgagttcct
      661 caggcacata ctcgagaagg gaaaggaagg ggtcaacatc cagaggtaca aaggtctcgg
      721 cgagatgaac gccgagcagc tctgggagac cacgatgaac cccgagaaga gaagggtcgt
      781 caaggtcacc atcgaagacg ccgtcgccgc ggatcaggtc ttcaccgtcc tcatggggaa
      841 caacatcgag acgcgcagga ccttcatcga ggagaacgcc ctgagcgtga gaaacctcga
      901 tatatgataa cgatcggcat aacggggatc atcggcagcg gcaagacgac cgcctcggcg
      961 gccctcaagg cccgcggcat tcccgtgata gacctcgatg ccctggccaa gaagatgctc
     1021 accctggcgg aggtccacga ggagatagaa cgggaattcg gcgatggtct cgtcatcgac
     1081 gggcagatag tggtcgagaa gctgagggat atcgtctttg cggacaggga gaagctgaga
     1141 aggctcgagc gcatcactca cccgcgcgtg cggcgcgagc tctggcggga ggtcggcgag
     1201 cgtgcggagg cgggagagaa ggcgattgtc gttgacggcc cgcttctctt cgagacgggt
     1261 ctttacaagg agctcgacag gaccgtcgtc gtcaccgccg gcatggacgt gatacgggaa
     1321 agattgaagc tgcggggcat ggcccccgac gacatagaga ggcggatatc caaccagata
     1381 ccgctcaaag aaaaggaaga ggcagcggac tatgtgttac tcaacaatgg aacgagagag
     1441 gacctcgaga ggaacatcga ggcacttctg gaaaggatca atgaatggga ggtaagaact
     1501 cgatgcacct caacgaccta aaaagcaaga aaatcggcga attaacacag atcgcgcggg
     1561 atctcaacat cgagaacgcc tcgggtctga gaaaacagga gctcatcttc tccatcctgc
     1621 aggcatatgt cgacaagaac gagaatgtgt acggggaagg ggttctcgag atactttccg
     1681 agggtttcgg gttcctgcgg tccacggatt ccaactatct tcccggcccc gacgacatct
     1741 acgtttcccc ctcgcagata aagaagttcg gcttacggac gggggacacg acgtcgggac
     1801 agatacggcc gccgaaggac aacgagaagt atttcgccct tctcaaggtg gagtccataa
     1861 acttcgacga ccccagcgtc gtgcgcgaca agatcatctt cgacaacctg acgccaatat
     1921 atcccaatga gagggcgaag ctcgagaccg tgcacgacaa cctctcgacg agggtcatgg
     1981 accttttcac gcccatcggc aagggacagc gcgggctcat cgtcgcgccc ccgagaacgg
     2041 gcaagacgat gctcctccag tccatcgcca acagcctgac gaagaaccac aaggagatat
     2101 acctcatcgt cctgctcatc gacgagcggc ccgaggaggt gacggacatg atgcgctccg
     2161 tcaagggcga ggtcataagc tcgaccttcg acgagcccgc gacacggcac gtccaggtcg
     2221 ccgagatcgt catcgagaag gcaaagaggc ttgtcgaaca caagaaggac gtcgtcatcc
     2281 ttctcgacag cataacacgt ctcgcccgcg cctataacac tgtggtgccc tcgagcggca
     2341 agatactctc cggcggtatc gacgcgtcgg ccatgcagaa gccgagacgc ttcttcggcg
     2401 ccgcgaagaa catcgaggag ggggggagcc tgaccatcat cgccaccgcg ctgatcgaca
     2461 cgggctcgcg catggacgag gtcatcttcg aggagttcaa gggcacgggc aactgcgaga
     2521 tatacctcga caggaaactg gcggagaaga ggatcttccc cgcgatagac atcaacaagt
     2581 cgggcaccag gaaggaagag ctgctcctcg gatcgagcga cctgcagcgc atctggctct
     2641 tgaggaaggt gctccagcca atgaacacgg tggaggccat ggaatttctc cttgaaaaat
     2701 tggccgatac ggattctaat aaagactttc tctattcgat gagcaaagga ggataagaga
     2761 catgaaaaag ggcatccacc cggaactcag gaccgcgatg gtgaagtgcg cctgcggtca
     2821 cacgtttgag accctttcag tgaaggacaa gatcaccgtc gagatatgcg ccaagtgcca
     2881 ccccatcttc acgggcaagg aaaaacgcct tgattccgcc ggtcaggtcg agaagttcga
     2941 aaggaaatac ggaaagaaac agaagtaatg ttcggcaaac tcattgagct tgagaaacgc
     3001 ttcgatgaaa tagaagcgga gatggtgaag cccgaggtgc tctccaacat ggaggagtac
     3061 aagaagcttg ccaaggagcg cacggacctc aaggaggtcg tcgatcccta ccgcgaatgg
     3121 aaggccgcga aggctgagca tgagaagacg tcggagcttc tgaagcagga gtccgacgag
     3181 gacatgaaga ccctggcgcg ggaggagctt gccagtctcc agaaggaaat ggaaaggatc
     3241 gaagggatcc tgacggacag cctcctgagg aagaccgaga agcaggcgaa gagcatgttc
     3301 ctcgagatac gggccggcac gggaggggag gaggccgccc tcttcgcgcg cgaccttttc
     3361 gcctcataca tgaaattcgc cgagcgcatg agatggaaga cggagatc
//
LOCUS       DAOQTR010000213         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_31925_length_3398_cov_3.322166, whole genome shotgun sequence.
ACCESSION   DAOQTR010000213 DAOQTR010000000
VERSION     DAOQTR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31925_length_3398_cov_3.322166"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PKN85_06090"
     CDS             <1..1356
                     /locus_tag="PKN85_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNT44011.1"
                     /translation="VSWAASPDGQAPVHPVPGPCAVLGWSRVTSHGTNRCGEPRVLLE
                     EPVKSMSIGFEEALRLTFEAIGPLPAESVGLLECVGRVAASDLFALVDSPSSDVSRKD
                     GYAVVYGEVKDATAAVPVRLSLAGSTSAGAAKNIVLTEGTTVRVLTGARVPAGADAVV
                     ADEYIRADGRDILIESPPDEPDRNILARGRDVRAGQRLIEAGRRISPAAAGFLAAAGL
                     GRVPVFSKPVVGIVGTGDEIVEPGAPLAEDRLYASNIVTLAGWCMRSGLRSLTAIVKD
                     DPDSILAAFRRMSMETDALITSGGAWKGDRDLVADVLERMGWRKIFHQIRMGPGKAVG
                     LGVLNGKPVFILPGGPPSNFMAFLQIAWPGLMRLGGHEEPGLPRVKAKLSAGITEGNA
                     GWTDLFFGTVEETEDLPLFHPMEKRSRLSSIAEATAVASIPEDRDHLAQGSVISVQLL
                     T"
     gene            1470..2585
                     /locus_tag="PKN85_06095"
     CDS             1470..2585
                     /locus_tag="PKN85_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNT44012.1"
                     /translation="MRKSTEHTRQLHRMYALDDERMKGHEGWEKFPLFWFILFLSYRC
                     TRTCEYCYAFNQVGYDNSVEMDDRTFERLLEWIPEVWRVNRTRVNIVVFLGGEPLLRT
                     DRIKNVMDAVYRNTDGMQGMVCTNADLVDSVNWDDLEDIQWISTNITDIPIKELARRM
                     KVIGERSNVINQTIIATLDEFNLERVLDITRFGIESGYRLRYYRNMYWGMDPRYRKAL
                     LERYHELCDMLEDYAIRGYDVHTTFLLDFLIPAWDLESSPYPCGKRMATVFPDGTIGP
                     CIRNHTFKSGTIYDPNPLAKLQVYDFHYDIRRPDLPDECKVCESRTACHAGCPNDKRM
                     FLDSTIGRSVLCDVHREIIPRLRHIESLKRNPSGTER"
     gene            complement(2587..>3398)
                     /locus_tag="PKN85_06100"
     CDS             complement(2587..>3398)
                     /locus_tag="PKN85_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNT44013.1"
                     /translation="AGESIKVGDTILNRGDRLTSAKVGILASLNHNTVSVYRQPTVSI
                     ISTGNELADIGEDMQFGQIRNVNAYTLHAEVGRYGGLPLYLGIVKDTMADAREVFLKA
                     LGSDVVISTGGVSMGKYDFVKDIFVDLGIDILFEWVKVKPGRPCTFGKKGDKLVFGLP
                     GNPVSSLTSFIQFVRPALLRLMGATRINKPVVNAVLDEDIKKQAGKVFLLRGRFTIRN
                     NELHVVTTGNQKSSVLRSMSEANCLIVIPEDTTRMKAGERVAIQLIDHDEL"
BASE COUNT          659 a         1001 c         1067 g          671 t
ORIGIN      
        1 gtcagctggg ctgcttcgcc tgatggccag gcgcccgtcc atccggtgcc gggtccctgc
       61 gctgtcttgg ggtggtcccg ggtgacctcc cacggcacga atcgctgcgg agagcccagg
      121 gtcttgctgg aggagcccgt gaagagcatg tctatcggct tcgaagaggc gttgcgcctg
      181 acgtttgagg cgataggacc tctgcccgct gagagcgtcg gtcttctcga atgtgtgggt
      241 cgcgtggccg cgtcggacct ttttgccctc gtcgactccc cctcgtcgga tgtttcccgg
      301 aaggatggtt acgcggttgt ctacggcgag gtgaaggacg ccaccgccgc ggttcccgtc
      361 cgtctctccc tcgccggttc cacctctgcg ggtgcggcaa agaacattgt cctcacggag
      421 gggacaacgg tgagggtgtt gaccggggcc agggtcccgg cgggggccga tgccgttgtc
      481 gccgacgaat acatcagggc cgacgggagg gacatcctta tagaatcgcc gcccgacgaa
      541 cctgacagga acatactggc ccggggcagg gatgtgcgcg ctgggcagcg cctgatcgag
      601 gcgggtcgcc gcatatctcc ggccgccgcc ggttttctcg ccgccgcggg tcttggcagg
      661 gtccctgtct tctcgaagcc ggtggtgggg atcgtcggga ccggcgatga gatcgtcgag
      721 cccggcgcgc cgcttgccga ggacaggctc tacgcgagca atatcgtcac cctggcaggg
      781 tggtgcatga ggtcgggact gagatcgctg acggccatcg tgaaggacga ccccgattcc
      841 atcctcgccg ccttccggag gatgtccatg gagaccgacg ccctcatcac cagcggcggg
      901 gcgtggaagg gcgatcgcga cctggtggcg gatgtccttg aacggatggg ctggagaaag
      961 atattccacc agatccgcat gggaccgggc aaggccgtgg gcctgggcgt gctgaacggg
     1021 aagcccgtct tcatccttcc gggagggccg ccgtccaact tcatggcatt cctgcagatc
     1081 gcgtggccgg gcctcatgcg cctcggcggc cacgaggaac ccggtctgcc ccgcgtgaag
     1141 gcgaagctct ccgcggggat cacggagggt aacgcgggat ggacggacct cttcttcggg
     1201 accgtcgaag agacggagga cctgcccctg ttccatccca tggagaaacg cagccgcctc
     1261 tcctccatag ccgaggccac ggcggtggca tccatacccg aagaccggga ccacctcgcg
     1321 caaggctcgg tgatctccgt ccagctgctg acataggact tcgggctgcc tgatccccgt
     1381 ccccttccgc caatcctctc agaacctcgt tgacttccat ggacctttct cctattaata
     1441 ttcgaagtca tcaaagaaag cgggtaattg tgagaaaatc gacggaacat acgagacagc
     1501 tccacaggat gtatgctctc gatgacgaaa gaatgaaagg gcacgagggc tgggagaaat
     1561 tccccctctt ctggttcatc ctcttcctgt catacaggtg cacgaggacc tgtgaatact
     1621 gctatgcctt caaccaggtc ggctacgaca acagcgtcga aatggatgac aggacgttcg
     1681 agaggctcct ggaatggatc cccgaggtct ggagggtcaa tcgcaccagg gtgaacatcg
     1741 tcgtcttcct tggtggtgaa cctctcctga ggacggacag gataaagaat gtgatggacg
     1801 ccgtctaccg gaacacggac ggaatgcagg ggatggtctg caccaatgcc gaccttgtgg
     1861 attccgtgaa ctgggacgac ctcgaagata tccagtggat ctcgacgaac atcaccgata
     1921 tccccatcaa agagctggcc aggaggatga aggtgatcgg cgaaaggtcg aacgttatca
     1981 accagaccat catcgccact ctcgatgaat tcaacctgga aagggtgctt gacataacac
     2041 ggttcgggat agagagcggg tatcgtctga ggtactacag gaacatgtac tgggggatgg
     2101 acccccggta caggaaagcg cttctcgaga gatatcacga actatgcgat atgctggagg
     2161 attacgccat caggggctat gacgtccaca ccacgttcct cctcgatttc ctcatccccg
     2221 cctgggacct ggaatcctcc ccctatccct gcgggaagag gatggcgacc gtcttccccg
     2281 atgggaccat cgggccttgc ataaggaacc acacgttcaa atcggggacc atctacgatc
     2341 cgaatccgct ggcgaagctg caggtctacg atttccacta cgatatcagg cggcccgacc
     2401 tgcccgacga gtgcaaggtc tgtgaatcga ggaccgcctg tcacgcggga tgccccaacg
     2461 acaagaggat gttcctggac agcaccatcg ggagatcggt cctctgcgat gtgcacaggg
     2521 agatcattcc gcggctaagg cacatcgaga gtctgaaacg aaacccttca gggacagaga
     2581 gatagcttac agctcgtcgt ggtcgatgag ctggatggcg acgcgttccc ccgccttcat
     2641 ccgggtcgtg tcttcgggga ttactatgag gcagttcgcc tcgctcatgg agcgcagcac
     2701 cgatgatttc tggttgcccg tggtgacgac gtggagttcg ttgttcctga tggtgaaccg
     2761 gccgcgcagg aggaagacct tcccggcctg tttctttatg tcttcgtcga ggacggcgtt
     2821 gacgacgggc ttgttgatcc tcgtcgcgcc catgagtctg aggagcgctg ggcggacgaa
     2881 ctgaatgaag gatgtcaggc tggagacggg gtttcccgga agcccgaaga ccagcttgtc
     2941 ccccttcttg ccgaaggtgc agggcctgcc gggttttacc ttcacccatt cgaagaggat
     3001 gtcgatgccc aggtcgacga agatgtcctt gacgaaatca tatttcccca tcgatacgcc
     3061 gcccgtggag atgacgacat ccgaccccag ggccttgagg aacacctccc tcgcgtccgc
     3121 catggtgtcc ttcacgatgc cgaggtagag ggggaggccg ccgtacctgc ccacctcggc
     3181 gtgcagggtg taggcgttga cgttccttat ctggccgaac tgcatgtctt cgccgatgtc
     3241 cgcgagctca ttgcccgtag agatgatgga gacagtcggc tgcctgtaca cgctcaccgt
     3301 gttatggttg agggacgcca ggatgcccac cttggccgag gtcaggcgat cgcccctgtt
     3361 gaggatggtg tcacccacct tgatgctctc cccggcga
//
LOCUS       DAOQTR010000214         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_31945_length_3397_cov_3.585278, whole genome shotgun sequence.
ACCESSION   DAOQTR010000214 DAOQTR010000000
VERSION     DAOQTR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31945_length_3397_cov_3.585278"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..412
                     /locus_tag="PKN85_06105"
     CDS             80..412
                     /locus_tag="PKN85_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44014.1"
                     /translation="MGGIDRKGLGVKGLIIVVGSCITCSAATVAAYDLFFAQKIVAVD
                     LASSIASQKEEYTAGKITADQLVENIEALLKRMEKRKRNEVLILEEAVAGDISHRSLG
                     NDGGGEEE"
     gene            584..805
                     /locus_tag="PKN85_06110"
     CDS             584..805
                     /locus_tag="PKN85_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44015.1"
                     /translation="MDEKNGKCPHCGGGNIIVGVRVDQTADAGRIGLAYKTKFVLGGT
                     EPFYADVCDDCGTITRIYVKETGKNWYRK"
     gene            complement(932..1906)
                     /locus_tag="PKN85_06115"
     CDS             complement(932..1906)
                     /locus_tag="PKN85_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HNT44016.1"
                     /translation="MMTGAQTRGRPRAIRAFVLWFTVLTALFLTACSTPPSLAEREGF
                     IEVTGGRVWYRIVGSGGATPVVVLHGGPGAASDYLKPLEKLAVDRPVIFYDQLGCGRS
                     DRPTDRTLWRIERFVEELAQVREALKLKEIHLYGHSWGTMLVVDYMLTKPFGVKSLIL
                     SSPCLSAKRWVEDADVLIAQLPPDTQKVIRHHEKEGTTDSKEYDDAVTEYLKRHLCRL
                     DPWPEDLTRSLASSNAEIYKKMWGPSEFHPTGDLRTYDRVDRLREITVPTLFTAGRYD
                     EATPGATTWYSSHLPGSTVRIFQKSSHMAMFEEQEEYMRTLREFMRQF"
     gene            2071..2574
                     /locus_tag="PKN85_06120"
     CDS             2071..2574
                     /locus_tag="PKN85_06120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HNT44017.1"
                     /translation="MGLRFDEVNDHVLSLLREVRSQDFPELVNAKIKVLFDLKKRKSG
                     GMIVLARIIKTNDLMRHLTKNGGDAIEGYDYIITLDKKCWDNTTDLDRVKILRHELRH
                     TFFDIESEDNPYKLLNHSISDFYEEVELNKNDPRWRERVATLTEDIYEQEKEARAEKK
                     NKKIKTA"
     gene            2813..>3397
                     /locus_tag="PKN85_06125"
     CDS             2813..>3397
                     /locus_tag="PKN85_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44018.1"
                     /translation="MMYSQEVEKMCSVTKGADHGPAPIPQEGKWTQVKEVKDICAFTH
                     GIGWCAPQQGTCKLTLNVKDGIIEEALVEALGCSGMTHSAAMAAEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRSQSAFSEGGLAIGAGLEDLGKGLRSQVATMYGTVAK
                     GPRYLEMAEGYVRKIALDDNNEIIGYEFVSLGRMM"
BASE COUNT          793 a          941 c          999 g          664 t
ORIGIN      
        1 gccaagcttc tcaacgtcag gatcatgata aagagcgccg gggaggaagc gggagcaaag
       61 gagggaggca tctgtgcgga tgggcggaat tgacagaaag ggcctcggcg tgaaagggct
      121 catcatcgtg gtaggctcgt gcattacctg ctcagccgcc accgttgccg cctacgacct
      181 cttcttcgcg cagaagatag tcgccgtgga cctcgcctcc tccatcgcgt cgcagaaaga
      241 agaatacact gccggcaaga tcacggcaga ccaactcgtc gagaacatcg aggctctcct
      301 gaaacggatg gaaaagagaa agaggaacga ggtcctcatc ctcgaggagg cggtcgccgg
      361 cgatataagc caccgtagtc tggggaacga cggaggggga gaggaggaat gatgcgagag
      421 tgcccggacg aatgggaaga ccgagacgaa acgatcgcta tcctcaatga tgaggagctt
      481 taaagagtat tcccgctttg cagagcttga catgcctcaa ccgacgttct aggctgtttc
      541 aactccccgc atcaggggac tggacgaaaa gagcggaggc gctatggacg agaagaacgg
      601 caaatgcccc cattgcgggg gcggcaacat catcgttggc gtaagggtcg atcagacggc
      661 ggacgcgggc aggatagggc tcgcctacaa gaccaaattc gtgctgggag gcacggaacc
      721 tttctatgcc gacgtctgcg acgactgtgg caccatcacc cggatctacg tcaaggaaac
      781 ggggaagaac tggtatagga aatgaagacc gttttaagtt ttaagttatt aggttttaag
      841 tgaaagcctt cgttcaacgg atgctcgcac gttcaacgtg acccgcggag cggtttcccg
      901 gtctttgacc taaaacgtaa taacttaaaa cttaaaactg tctcataaac tcccgtaatg
      961 tcctcatgta ctcttcctgc tcttcgaaca ttgccatgtg ggaacttttc tggaagatcc
     1021 gcacggtgga acccgggaga tgggagctgt accaggttgt agcgccgggg gttgcctcgt
     1081 cgtaccggcc cgcggtgaag agggtgggca ccgtgatctc ccggagccgg tccaccctgt
     1141 cgtatgttct gaggtcgccc gtggggtgga attcgcttgg tccccacatc ttcttgtaga
     1201 tctccgcgtt cgacgaggcc aatgaccgtg tcaggtcctc cggccagggg tcgagacggc
     1261 acaggtgccg tttgaggtac tccgtgacgg catcgtcgta ttccttcgag tccgtggtgc
     1321 cctctttctc gtggtggcgg atcaccttct gcgtatcggg tggaagctgc gcgatgagga
     1381 catccgcgtc ctcgacccat cgctttgcgc tgaggcacgg gcttgagagg ataagacttt
     1441 tcaccccgaa aggcttcgtc agcatgtagt ccacgaccag catcgttccc caggagtgtc
     1501 cgtagaggtg gatctctttg agtttcagcg cctcgcgcac ctgggccaac tcctcgacga
     1561 acctttcgat gcgccagagc gtcctgtccg tcggtcggtc cgacctgccg cagcccagtt
     1621 gatcgtagaa gatgaccggt ctgtcgacgg cgagcttctc aaggggtttg aggtagtcgc
     1681 tcgcggctcc cggcccgcca tggagcacga ccacgggggt ggcgccgccg gagccgacga
     1741 tgcggtacca caccctgcca cccgtgacct ctatgaaccc ctcgcgctca gcaagggagg
     1801 gaggagtgga gcaggcggtg agaaagagcg cggtcaggac cgtaaaccat aggacgaagg
     1861 ccctgatcgc ccgcggcctg cctcgtgtct gtgcacctgt catcatcatg tcctcccttt
     1921 tttagcggtg tgcccgtcat gttccttata tcggttgaac cccttccgtg tcaagaatgt
     1981 cacccggggg aacggtccct tgctttttgg caccccctca tggtaaattt ccccgtccgc
     2041 ttaagatccg tacgtatttc aggaggcacc atgggattgc gctttgacga agtgaacgac
     2101 cacgttctga gcctgctcag ggaggtgagg tcgcaggatt tccccgaact ggtcaacgcg
     2161 aagataaagg ttctctttga cctgaaaaag aggaagtcgg gcgggatgat cgtcctcgcc
     2221 cggatcataa agaccaacga ccttatgcgc cacctcacga agaacggggg cgacgccatc
     2281 gaagggtacg attacatcat caccctcgac aagaaatgct gggacaacac caccgatctc
     2341 gacagggtca agatactgcg ccacgaattg cggcacacct tctttgacat agagtccgaa
     2401 gacaacccgt acaagcttct caaccacagc atatccgact tctacgaaga ggtcgagctc
     2461 aacaagaacg acccccgctg gcgcgaacgc gtcgccaccc tcaccgagga catctacgaa
     2521 caggaaaaag aagccagagc ggaaaagaag aacaaaaaga taaagaccgc ttgaatggct
     2581 tgagtcgctt gagagtcaag ggcttccaga cctcttcttt gaaagaagaa ggttattcgt
     2641 ctttgccttt aaagccattc aagctattca agcgactcaa gccgtcaagc tattaaagcc
     2701 gtttccccct ctttccccca tgacatcagc cccgccaagg tagtatacta taggcgcttc
     2761 gtcttttcac gttgactaac cgcaagaaac caaccaagga ggatatacgc tcatgatgta
     2821 ctctcaggaa gtggagaaga tgtgctccgt gacgaaagga gcggaccacg gcccggcgcc
     2881 gataccgcag gagggcaagt ggacccaggt aaaggaagtt aaggatatat gcgcgttcac
     2941 gcacggcata ggctggtgcg ccccccagca ggggacgtgc aagctgacgc tcaacgtaaa
     3001 ggacgggatc atcgaggagg ctcttgtcga ggcgctcgga tgttcgggca tgacccattc
     3061 ggctgccatg gccgccgaga tactgccggg caagaccata ctcgaggcgc tcaacacgga
     3121 cctcgtgtgc gacgcgatca atacggcgat gagggaactt ttcctccaga tcgtttacgg
     3181 caggagccag tcggcattct ccgaaggcgg tctggcgata ggcgccgggc tcgaggacct
     3241 gggaaagggc ctgcgcagcc aggtggccac catgtacggc accgtggcaa agggcccccg
     3301 gtacctcgag atggccgagg gctacgtgag aaagatagct ctcgacgaca acaacgagat
     3361 aatcggctac gaattcgtca gcctgggcag aatgatg
//
LOCUS       DAOQTR010000215         3380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_32161_length_3380_cov_2.893233, whole genome shotgun sequence.
ACCESSION   DAOQTR010000215 DAOQTR010000000
VERSION     DAOQTR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32161_length_3380_cov_2.893233"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="PKN85_06130"
     CDS             complement(<1..1596)
                     /locus_tag="PKN85_06130"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT44019.1"
                     /translation="MYKPTSPQQRLFGIESRVSPSLRKRLESSWAELFRNEILPILFR
                     NEDRYAILYGSTGRPNFSVARLLGLCLLQEWNDLSDQETLDTFSFDIRWRYALDVNDD
                     EDYLSRRSLVEFRRRLVVSDPDMRLIRDLFDTVRDSAIRKMGLSVSDQRLDSTLVVSN
                     IRVKGRLALFSDTLTLFLKSLDTERLARVPEAIRTWYSSEPEGWFGLGFSEQTVRLEE
                     LAQYLHDLVCLFEDCTDEPYLLLKRLFSEHCGMNGTSVHLKKRIEGASLQSPYDPDAS
                     FGHKGSGYSVHVTETCGNDGKPEIITDYEVHGAARSDMGKALDVIERLDTAGLKPDIL
                     FADGGYPSVPSASKIVDRGTELMAPVNRSRLGDDVIGRDLFVFDDEGRVVRCPMGHAP
                     IDHRVLSRNNTVRRSLHAIFDGDTCRSCAMLDQCPVRTPNHRARGCKARDSVGDFRLD
                     ISREIRLRDEMYAAQHTREWKDRYSIRTGIEGTNSELKRAHGMMRIRLRRLAKVCFSV
                     ACKITACNIKRWARAHAAAGGPLW"
     gene            complement(1651..2910)
                     /locus_tag="PKN85_06135"
     CDS             complement(1651..2910)
                     /locus_tag="PKN85_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT44020.1"
                     /translation="MEEKRPASSKVVLVMAAPVAAMAFAAIYLIVRVILFMITDHVWY
                     EKALAFLLLMAEIFILVHGIGYFLEILSVAWTRREFARKELPVEPLSSYPPVAIVMPS
                     YKEPLEVVKNSLITFYNISYPNKYIYFLDDTRYDVPWDTPENMEAYKRSIEELCQYVG
                     VDLFRRKWHGAKAGIINDFMEHLDGQMKEGFIFYNYSKKIKKETERYMVVFDADSNPF
                     PDFLEPLVARMENSPGLAFIQTPQYYINFDSNRIARAAGLQQTVFYEYICEGKASKDA
                     TFCCGTNVLFRREALKSVGGFDESSVTEDFATSLQFHLNKWKTTYINRVRTFQIGPED
                     LGGYFKQQFRWSLGTIGLLRKVITTFILRPRALTLLQWWEYLLSSTWYFIGFVFFIMM
                     ICPPIYLFFNVPVYFARPDFYLLIPEK"
     gene            complement(2912..>3380)
                     /locus_tag="PKN85_06140"
     CDS             complement(2912..>3380)
                     /locus_tag="PKN85_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44021.1"
                     /translation="EVSGKAEYIGYNDSNSGSLFSLAAGYALTDHPRTFKVAASGETR
                     NTRHDNEYVYRNGELVDIIHPYWAPRDYLAGSVLLEWRHDLSKTFICGAEQHFYDLKA
                     SFGTDSENNPYAKIEGEWSWEFLKHWLVGIKALAHTSPKWDATGAWAWLRYRF"
BASE COUNT          712 a          910 c         1006 g          752 t
ORIGIN      
        1 ccataacggc cctccggcgg ctgcgtgggc cctggcccac ctcttgatgt tgcatgctgt
       61 tatcttgcat gccacagaga agcagacctt tgccagccgg cgcagacgga tccgcatcat
      121 tccgtgagca cgtttgagtt ccgagttcgt tccctcgatc cctgttcgaa tgctgtaccg
      181 gtccttccat tcacgggtgt gctgcgcagc atacatctca tcccgcaggc ggatttcccg
      241 ggatatatcc agccggaagt cgccgacgct gtcgcgcgct ttacatcccc gggccctgtg
      301 gttgggagtg cgaacagggc actgatcgag catcgcacat gaacggcagg tatcgccgtc
      361 gaagatcgca tggagggaac gccgcacggt gttgtttctg ctcagcacac ggtggtctat
      421 cggagcgtgg cccatgggac aacggaccac ccgaccctcg tcatcaaaga caaagaggtc
      481 cctgccgatg acatcgtcgc cgagtctgct gcgattgaca ggagccatga gctctgtacc
      541 cctgtcgacg atcttcgatg cagacggcac ggaaggatat cctccgtcgg caaaaagtat
      601 gtccggtttg agaccggcgg tgtcgagccg ttcaatgacg tccagggcct tgcccatatc
      661 cgaccgggca gcgccatgca cctcgtagtc ggtgatgatc tccggttttc cgtcattgcc
      721 gcaggtctcg gtcacgtgga cgctgtatcc cgaccctttg tgtccgaagg acgcgtccgg
      781 gtcataaggg gattgaaggg aagctccttc aatccttttc tttagatgta cggatgtacc
      841 gttcatgccg cagtgttcgg agaagaggcg cttgagcagg agatacggtt catcggtgca
      901 gtcctcaaag aggcatacga ggtcatgaag atactgtgcc agttcctcga gcctcaccgt
      961 ctgttcagaa aaaccgagtc cgaaccagcc ttcgggttcc gatgaatacc atgtacgtat
     1021 tgcttcgggt acccgcgcca ggcgctcggt atcgaggctt ttgagaaaca gtgtgagcgt
     1081 gtccgagaac agggcaagcc ttcccttgac cctgatgttc gatacgacaa gcgttgaatc
     1141 gagcctctga tcgctcaccg aaagacccat cttccttatt gcggagtccc tgacggtatc
     1201 aaacagatcg cggatgagcc tcatgtcggg gtcgctgacc acaaggcgtc tgcggaactc
     1261 gacaagagag cggcgggaca gataatcctc gtcatcgttc acgtccaggg catagcgcca
     1321 cctgatgtca aagctgaagg tatcgagggt ctcctggtcg ctcaggtcgt tccattcctg
     1381 caggagacac aggcccagaa ggcgggcgac gctgaagttg ggccggccgg tagaaccgta
     1441 cagaattgca taccggtctt cgttcctgaa caggatgggg agtatttcat ttctgaacag
     1501 ctcagcccat gaagactcaa gacgcttacg cagggatggt gatacccgac tctcgatacc
     1561 gaataatctc tgttggggtg atgttggctt atacatctgt cattatatca ctattgtaat
     1621 agtagtccaa ccataaatct cctaatcgag ttacttctca ggaatcagga ggtagaaatc
     1681 ggggcgcgcg aaatacacgg gcacattgaa aaagaggtag atgggggggc atatcatcat
     1741 gatgaagaag acgaagccga tgaaatacca cgtgctcgac aggagatatt cccaccactg
     1801 aaggagcgtg agggcccgag gccggaggat gaaggtcgtt atgaccttcc tcaggagtcc
     1861 tatcgtcccc agggaccacc tgaactgctg tttgaaatag cccccgaggt cctccgggcc
     1921 tatctggaag gtgcggaccc tgttgatgta cgtcgtcttc cacttgttga ggtggaattg
     1981 aagtgaagtg gcaaagtcct cggttacgga ggactcgtcg aatccgccca cgcttttcaa
     2041 ggcctcacgg cggaaaagga cgtttgtgcc gcagcagaag gtggcgtcct tcgatgcctt
     2101 gccctcgcag atgtactcgt agaagacggt ttgctgcagt ccggcggcgc gggcgatgcg
     2161 gttggaatcg aagttgatgt agtactgcgg cgtctggatg aaggccagtc cggggctgtt
     2221 ctccatccgg gcgacgaggg gttccaggaa atcggggaac ggattggagt cggcgtcgaa
     2281 gacgaccatg tatcgctccg tctctttctt tatctttttg gaataattgt agaatatgaa
     2341 cccctctttc atctgtccgt cgaggtgttc catgaaatcg ttgatgatac ccgccttcgc
     2401 cccgtgccat ttcctgcgga agagatcgac gccgacgtac tggcaaagct cttcgatgga
     2461 cctcttgtat gcctccatgt tctcgggggt gtcccagggg acgtcgtatc gggtatcgtc
     2521 caggaagtag atgtacttgt tcgggtacga gatgttgtag aaggtgatga gagagttctt
     2581 gacgacctcg agaggctctt tgtaggaggg catgactatg gccacgggcg gataggatga
     2641 caggggttcc acggggagtt cctttcgggc gaactccctg cgtgtccatg ccacggagag
     2701 gatctcaaga aagtacccga tgccgtggac gaggatgaat atctccgcca tgaggaggag
     2761 gaacgcgagg gccttctcgt accacacatg atcggtgatc atgaagagaa tgacgcgcac
     2821 gatgaggtag atggcggcga aggccatcgc tgcgacggga gccgccatga caagaaccac
     2881 ttttgaagat gccgggcgct tttcttccat ctcagaaccg gtacctcagc cacgcccatg
     2941 cgcccgtggc gtcccatttc ggggacgtgt gggccagggc ctttatacca acaagccagt
     3001 gcttcaggaa ctcccagctc cattcaccct cgatcttcgc gtagggattg ttctccgagt
     3061 ccgtgccgaa ggaggccttg agatcgtaga aatgctgctc cgccccgcag atgaaggtct
     3121 tcgagaggtc gtgccgccat tcgaggagga cgctgccggc gagataatcg cgcggcgccc
     3181 agtaggggtg gattatgtcg acgagctccc cgttgcggta gacgtactcg ttgtcatggc
     3241 gcgtattgcg cgtctctccg gaggctgcca ccttgaaggt ccgcgggtgg tcagtaaggg
     3301 cataccccgc cgcgaggctg aagagactgc cgctgttgga gtcgttgtaa cctatgtact
     3361 ccgccttgcc gcttacctcg
//
LOCUS       DAOQTR010000216         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_32283_length_3371_cov_3.514475, whole genome shotgun sequence.
ACCESSION   DAOQTR010000216 DAOQTR010000000
VERSION     DAOQTR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32283_length_3371_cov_3.514475"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKN85_06145"
     CDS             <1..342
                     /locus_tag="PKN85_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT44022.1"
                     /translation="RQGATIIIFPEGTRSADGKLLPFKKGGFTLALRAMVPVVPVGIF
                     GSSALQPKGCFIPLEKGIIYIEVGEPIVLEGMDRSQKTQVMDDVRVRIEELIAKGESS
                     GGEGGRERPGP"
     gene            356..2401
                     /locus_tag="PKN85_06150"
     CDS             356..2401
                     /locus_tag="PKN85_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNT44023.1"
                     /translation="MNLSADLKKEMLGVALIGVFLFFFVSLLSYHPLDPSFHTVTDAA
                     AKNLCGKAGSYLSDLFIQLFGLMSYLLVGYSLVFGIFYVRKKDPPSIIVFSSGLVLFF
                     LSTGTLLQVFAGKVSLRGIEVEFSGFMGSLLEKALMNFLGSFGTILVAVILLLISAFL
                     IIQAPILGVIEDRIARRKKAERRREIKVTQEKKEEETPAKAPEKKAAVQETFEFFKEI
                     GPYKLPDPSLLDDVQKKEMKVDKDSIQANASILEKKLKDYGIEGRVTEVRPGPVITMY
                     EFEPAPGVKVSKIANLSDDLAMALSAVSIRIIAPIPGKAVVGIEIPNKERETVYIREI
                     IESKVFTSSPSHLTLVLGKTINGDSYVADLAKMPHLLVAGATGSGKSVSLNSMILSIL
                     FKATPAHVRFLMIDLKMLELSFYEGIPHLLLPVVTNAKNAKTALRWMTDEMERRYNMM
                     AQKGVRNIEKYNHKVIREDGEALPYIVVVIDELADLMMVSPKEVEEYIARLAQMARAS
                     GIHLILATQRPSVDVLTGIIKANFPARVACKVFSKVDSRTILDTNGAESLLGYGDMLF
                     LSPGIGRLQRLHGSYVSEGEIKHIVEFLKQQGAPSYHHEILEEKDDEEDGGETIDDEK
                     YQEAVEFVSSRGEASISMVQRRFRIGYNRAARIIERMEQEGVVGPSDGVKPREVLKK"
     gene            2422..3111
                     /locus_tag="PKN85_06155"
     CDS             2422..3111
                     /locus_tag="PKN85_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNT44024.1"
                     /translation="MKDRALRRTRNLSSPVLFGVLAVFLLIPFFAGGDATSAAKGETG
                     SPFDSIKKTYAGITALEASFHQKIFVSGIKKIRDFDGNFYFKRHKGFLWKYTKPKGKT
                     FLYDGHYMWQDEEGKTFVLKSKVSREKTGGTFFDLVEDIARIDNLFTVKQHSMLAGMQ
                     YFELLPKKDSTVNIAKIWIDKNNLLRKIEILEFTGNVNTIEFTDIKVNAPVSDSKFTY
                     KSDGIKDVVER"
BASE COUNT          762 a          946 c          973 g          690 t
ORIGIN      
        1 cggcaaggcg ccaccatcat catctttccc gaaggtacgc gaagcgccga cggaaagctc
       61 cttcctttca agaagggggg gttcaccctg gcccttcggg ccatggttcc cgtcgttccc
      121 gtcggcatct tcggttccag cgcgctgcag ccgaaagggt gttttattcc cctggaaaaa
      181 ggcataatat acattgaggt cggtgagccc atcgttctcg aaggcatgga caggtctcaa
      241 aagacacagg tgatggacga cgtgagggtc cgcatcgagg aacttattgc aaaaggcgaa
      301 tcctcgggcg gggaaggagg ccgggaaagg cccggcccat gacgatggca ggcgcatgaa
      361 tctatcggcg gatctgaaga aggagatgct cggggtcgcc ctcatcgggg tgttcctctt
      421 ttttttcgtc agcctcctct cctaccaccc ccttgacccc tcgttccaca ccgtcaccga
      481 tgccgcggcg aaaaatctct gcggcaaggc cggctcgtac ctgtcggacc ttttcataca
      541 gctttttggc ctcatgagct atcttctcgt cggttactcc ctggtctttg ggatcttcta
      601 cgtcagaaag aaggatcccc cgagcatcat cgtcttctcg tcggggctcg ttctcttctt
      661 tctctccacg ggaacgctcc ttcaagtctt cgcgggaaag gtctccctgc gggggattga
      721 ggtggagttc tcggggttca tggggtcgct tctggaaaag gcgctcatga acttcctcgg
      781 cagcttcggc accattctcg ttgccgtaat cctccttctc atctccgcct tcctcatcat
      841 tcaggcgccg atactggggg tgatcgaaga ccgcatagcg aggcgaaaga aggcggaacg
      901 ccgtcgtgag ataaaggtga cccaggagaa gaaggaagag gagacgccgg cgaaggcgcc
      961 ggagaagaaa gcggccgtgc aggagacctt cgagttcttc aaggagatcg ggccctacaa
     1021 gctgcccgac ccgtcccttc tcgacgatgt gcagaagaag gagatgaagg tcgacaagga
     1081 ctccatccag gccaacgcct ccatcctgga gaaaaagctc aaggattacg ggatagaggg
     1141 cagggtcacc gaggtcaggc ccggcccggt catcaccatg tacgagttcg agcccgctcc
     1201 gggggtcaag gtgagcaaga tagccaatct ctcagacgat ctggccatgg cgctcagtgc
     1261 cgtttccatc aggatcatcg cccccatacc cggcaaggcc gtggtcggca tcgagatccc
     1321 gaacaaggaa cgggagacgg tgtacatccg ggagatcatc gaatcgaagg ttttcacctc
     1381 gtccccgtca cacctgacgc tcgtgctggg aaagacgata aacggcgatt cctacgtggc
     1441 ggatctggcg aagatgcccc accttctcgt cgccggggcg acggggtccg gaaagagcgt
     1501 gtccctgaac agcatgatcc ttagcatcct tttcaaggcg acccccgctc acgtccgttt
     1561 cctcatgata gatctcaaaa tgctcgaact gtccttctat gagggcattc cccaccttct
     1621 cctacccgtc gtgacgaacg cgaagaacgc gaagacggcc ctgcgctgga tgactgacga
     1681 gatggagagg cgctacaaca tgatggcgca gaagggtgtc cgcaacatag agaaatacaa
     1741 ccacaaggtc atccgggagg acggcgaggc cttaccctac atcgtcgtcg tgatcgacga
     1801 acttgccgac ctcatgatgg tctccccgaa agaggtggag gaatatatcg cccgcctcgc
     1861 gcagatggct cgtgcgtcgg gtatccatct catccttgcc acccagaggc cctccgtcga
     1921 tgtcctgacg ggcatcatca aggccaattt ccccgcccgg gtggcgtgca aggtcttctc
     1981 caaggtggat tcccggacca tactcgacac gaacggcgcc gagagtcttc tcggctacgg
     2041 ggacatgctt ttcctgagcc ccgggatcgg gcggctccag cgcctccacg ggtcctatgt
     2101 ctccgaaggc gagatcaagc acattgtgga attcctgaag cagcaggggg cgccctccta
     2161 tcatcacgag atcctggaag agaaagacga tgaggaagac ggcggcgaga cgatagacga
     2221 cgagaaatat caggaggctg tcgaattcgt gtcatcgagg ggagaggcat cgatcagcat
     2281 ggtccagagg aggttccgca ttggatacaa ccgggcggcc cgcatcatag aacggatgga
     2341 gcaggaaggt gttgtgggtc cctccgacgg tgtgaagccg agggaggtcc tgaagaaatg
     2401 atccttgaga ggtatggtcg aatgaaagat agagctcttc ggagaacaag aaacctgtca
     2461 tcgccggtat tgttcggcgt tctggcggtg ttcctgctta ttcccttttt cgccggcggg
     2521 gacgcgacgt ccgccgctaa gggcgagacc gggtctccct tcgattccat aaagaagacc
     2581 tatgccggca tcaccgcgtt ggaggcctcc tttcatcaga agatcttcgt ttccggcatc
     2641 aagaagatcc gcgatttcga cgggaacttc tatttcaagc ggcacaaagg gttcctctgg
     2701 aagtacacga agcccaaggg caagacgttc ctctatgacg ggcattacat gtggcaggat
     2761 gaggaaggca agaccttcgt gctcaagagc aaggtcagca gggagaagac ggggggcacc
     2821 ttctttgacc tcgtcgagga catagcacgc atcgacaacc tcttcaccgt caagcaacac
     2881 agcatgctgg cggggatgca gtacttcgag cttctcccga agaaggattc caccgtcaac
     2941 attgccaaga tctggatcga caagaacaat ctcctgagga agatcgagat cctcgaattt
     3001 accggcaacg tcaacacgat cgagttcacc gacatcaagg tgaacgctcc cgtcagcgac
     3061 tcaaaattca cctacaagtc cgacggcatc aaggatgtgg tggaaagata aaagaccgtt
     3121 tcaggtttca ggcgaaagac ggttgcgggt tccgggttgc gggtcaaagg ccaaagaggg
     3181 accctttctt gactggccca accaatacag ttgcaaaggt tccggtattg gctcgaacct
     3241 gaaacgtgaa acgtgagacc tgtgacctgc gacttgggac ggtttctcgt cagagaaacc
     3301 tttttttcag ttcctttacc agcgcgatgt tctccacgtg gcctgtctgt cgggcggtta
     3361 tcttgcccgt t
//
LOCUS       DAOQTR010000217         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_32438_length_3358_cov_3.850439, whole genome shotgun sequence.
ACCESSION   DAOQTR010000217 DAOQTR010000000
VERSION     DAOQTR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32438_length_3358_cov_3.850439"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PKN85_06160"
     CDS             <1..521
                     /locus_tag="PKN85_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNT44025.1"
                     /translation="DFKNGVTTTKDVVDIKGEKVDMRGTGLLANTNEETLRLLSDVGG
                     IIATEKAKYRFQSDSLLYYFQKATYVLEGKVILKGEDLNMVCDLVHIYSTDNEVDRIE
                     ADGNVRVISKGTVAKSEKAVYHFKEGRIVFTQSPRVFKDNVEMKGERVIYTTEDGKLS
                     VERPKIRMERGK"
     gene            527..1264
                     /gene="lptB"
                     /locus_tag="PKN85_06165"
     CDS             527..1264
                     /gene="lptB"
                     /locus_tag="PKN85_06165"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNT44026.1"
                     /translation="MKATLSAQGLLKTYNARKVVDGISMYMETSEIVGLFGPNGAGKT
                     TSFYMIIGFIKPNGGKILLDGEDITDLPMFMRARKGITYLPQEPSVFKKMSVRDNLKA
                     ILEFLGEESDLINHRVMEILESFKLEHLANNSADSLSGGERRRLEIARALMTSPHFML
                     LDEPFSGIDPISVSDLKKIIFGLKQRGIGILLSDHNVRESLPICDRAYVVNAGKVLLE
                     GTPESVAQDRIVREVYLGEEFNIDVGA"
     gene            1251..2621
                     /gene="rpoN"
                     /locus_tag="PKN85_06170"
     CDS             1251..2621
                     /gene="rpoN"
                     /locus_tag="PKN85_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNT44027.1"
                     /translation="MLELKQTQKLSPVLTQQLQQAIRLLQLSKLELVEAIELEMKENP
                     ILEITEEEPREDAEEKESQDEKREMEELLERYSPSEEFFPAEDREAPDYENMVRKTYN
                     LRDHLRWQIGLSDFSPRERVVAEWIIENIDDNGYLICGLEEISRDSGFPVEVLEPVLR
                     KVQRLEPAGVGARDLKECILIQYEVSGEKDPVFENVVANHFELFQHNNLRGIAKATGF
                     PIERIKEVFEKIKSFDPKPGRNFSDEFISYVVPDVYVVKGEEGFDIALNNDDVPELRM
                     SRYYLELATDRKVSGETKRYIKNKMKQAEWFIKSIQQRQRTLFLVAQSIVNFQREFFE
                     NGLRSLRPLILKDVAQDVGVHESTVSRITTSKYMSTPHGVYEMKFFFPTSINKGEGDP
                     LSTNVVMDLIAELVKKEDKKKPLTDDEIAHILKSRHDINIARRTVAKYREILNIRSSR
                     ERTVID"
     gene            2785..3309
                     /gene="raiA"
                     /locus_tag="PKN85_06175"
     CDS             2785..3309
                     /gene="raiA"
                     /locus_tag="PKN85_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNT44028.1"
                     /translation="MDITTSFRHVDTSEDVRRYVEEKLSRLQKYVETPLDIHVVISLE
                     RKYRQRIDVMFTINGVVINAHEVMDDIYAAIDSILDKLERRLTKYRDKMKEYRESKLK
                     RTTPPPTSEESGIIVSRTVEAKPMDPEEAVMQLTASGDMFMVFRERERDNVCIVYKRK
                     DGKFSLIEAAGKGP"
BASE COUNT          887 a          898 c          976 g          597 t
ORIGIN      
        1 tcgatttcaa gaacggcgtc acgacgacaa aggatgtggt ggacatcaag ggagagaagg
       61 tcgacatgcg cggcacgggg cttctcgcca acaccaacga ggagaccttg cggctcctct
      121 ccgacgtcgg cggcatcata gcgacggaga aggccaagta tcgcttccag tcggactccc
      181 tcctttatta ctttcagaag gccacctatg tcctcgaggg aaaggtcatc ctcaagggag
      241 aggacctcaa catggtgtgc gaccttgtcc acatatacag caccgacaac gaggtggacc
      301 gcatcgaggc cgacggcaac gttcgcgtca tatccaaggg cactgttgcg aaaagcgaaa
      361 aggcagtata tcattttaag gaaggcagga tagtgttcac ccaatctccg agggtattca
      421 aggacaacgt ggaaatgaaa ggggagcgtg tgatctacac gacggaggac ggcaaattgt
      481 ccgtcgagag gccgaagata agaatggaga ggggaaaata gcaggaatga aagcaacgct
      541 gtccgcgcag ggtctcctga agacatacaa cgcgcgcaag gtcgtcgacg ggatcagcat
      601 gtacatggag accagcgaga tcgtggggct cttcggcccc aacggagccg gaaagacgac
      661 gtctttctac atgatcatcg gcttcatcaa gcccaatggc ggcaagatcc tcctcgacgg
      721 ggaggacatc accgacctgc ccatgttcat gagggcgcgc aagggcataa cctatcttcc
      781 ccaggaacct tcggtcttca agaagatgtc cgtcagggac aacctcaagg cgatcctcga
      841 gttcctcggt gaggagagcg acctcataaa ccaccgcgtc atggagatac tcgagtcctt
      901 caagctggaa cacctggcga acaacagcgc cgattccctc tcgggcggag agagaaggcg
      961 cctcgagata gcgcgggcgc tcatgacatc gccgcatttt atgctcctcg acgagccttt
     1021 ctccggcata gatcccatat ccgtctcgga cctgaaaaag ataatcttcg ggctcaagca
     1081 aagaggcatc ggcatactgc tgtccgacca caacgtccgt gagtcgctcc ccatatgcga
     1141 cagggcatac gtggtcaacg cgggaaaggt ccttctcgaa ggcacgccgg agagcgtggc
     1201 ccaggaccgg atcgtccgcg aagtatatct tggtgaggaa ttcaacatcg atgttggagc
     1261 ttaaacagac acagaagctc tcgcccgtcc tgacccagca gctgcagcag gcaatccgcc
     1321 tccttcagct ctccaagctg gagcttgtcg aggccatcga gctcgagatg aaggagaacc
     1381 ccattctcga gatcaccgaa gaagaaccgc gcgaggacgc cgaggaaaag gagagccagg
     1441 acgagaagag agagatggag gagctcctgg aacgatactc gccctcggag gagttcttcc
     1501 ccgccgagga cagggaggcc cccgactacg agaacatggt caggaagacg tacaacctca
     1561 gggaccacct gaggtggcag atcggccttt cggacttcag cccacgggaa cgggtggtgg
     1621 cggaatggat catcgagaac atcgacgaca acgggtacct catctgcggc ctcgaggaga
     1681 tatcgcggga ttccggtttt cccgtcgagg tcctcgagcc ggtcctcaga aaggtgcaga
     1741 ggctcgagcc ggccggggtg ggcgcgcggg acctgaagga atgcatcctc atacagtacg
     1801 aggtgtcggg tgagaaggac cccgtcttcg agaacgtcgt cgccaatcac ttcgaactct
     1861 tccagcacaa caacctcagg gggatagcaa aggcgaccgg tttccccata gagaggatca
     1921 aggaggtctt cgagaagatc aagtccttcg atcccaaacc gggaaggaac ttcagcgacg
     1981 agttcatatc ctatgtcgtc cccgacgtct atgtggtcaa gggcgaggaa gggttcgaca
     2041 tcgccctcaa caacgacgat gtcccggagc tcaggatgag ccgctactac ctggagctcg
     2101 cgacggacag gaaggtgagc ggcgagacga agaggtacat aaagaacaag atgaagcagg
     2161 cggagtggtt catcaagagt atccagcaga ggcaaaggac gctctttctc gtcgcccaga
     2221 gcatcgtgaa cttccagcgt gagttcttcg agaatggcct gagaagctta aggcccctta
     2281 tcctcaagga cgtcgcccag gatgtggggg tccacgaatc gacggtaagc cgcataacga
     2341 cgagcaagta catgagcacc ccccacggtg tgtacgagat gaagttcttc ttcccgacga
     2401 gcatcaacaa gggagaaggg gaccctcttt cgacgaatgt cgtcatggac ctcatagcgg
     2461 agctcgtcaa gaaggaagac aagaagaagc ccctcacgga cgatgagata gcccacatcc
     2521 tgaaatcgcg ccatgacata aacatagccc gccgtacggt cgcgaaatac agggagattc
     2581 tcaacattcg gtcctcacgg gaaaggacgg tgatagacta aaggcacaga ttccggatac
     2641 ggggaagcct tggcggagcg tttacgctcc tgatcgatta acgtaatact ttcggggaat
     2701 tggattgaca cgatccggca tatctgttat taataagctc caaggttggt ccacgccaac
     2761 cttatttttg taagcggagg aactatggat atcacaacca gcttcaggca cgttgatacc
     2821 agcgaagatg taaggcgtta tgtcgaggag aagctctccc gtctgcagaa atacgtggag
     2881 acgcccctcg acatccacgt ggtcatctcc ctcgagcgga aataccgcca gcgtatcgat
     2941 gtcatgttca ccatcaacgg ggtcgtcatt aatgcccatg aggtcatgga tgatatctat
     3001 gcggcgatcg acagcatact cgacaagctg gaacgaagac ttacaaagta ccgcgacaag
     3061 atgaaggagt accgcgagag caagctgaaa cgcaccacac cgccgcctac gagcgaggag
     3121 tcgggcatca ttgtatcgag gaccgtggaa gccaaaccca tggaccccga ggaggccgtg
     3181 atgcagctta cggcctcggg ggacatgttc atggtcttcc gcgagaggga acgcgacaac
     3241 gtctgcatcg tttacaaacg gaaggacggg aagttcagtc tcatagaagc ggcagggaag
     3301 ggaccgtgag gttaaccgac atactgaaag aatcctcgat catctccgat atcaaggg
//
LOCUS       DAOQTR010000218         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_32443_length_3358_cov_3.076294, whole genome shotgun sequence.
ACCESSION   DAOQTR010000218 DAOQTR010000000
VERSION     DAOQTR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32443_length_3358_cov_3.076294"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1505)
                     /locus_tag="PKN85_06180"
     CDS             complement(276..1505)
                     /locus_tag="PKN85_06180"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT44029.1"
                     /translation="MKTSLFDRKIFSWCLFDFANSSYSAVIAAVIFPVYYTTVVVGND
                     ANLGDLWWGRAISVSMAFVALSSPFLGGISDYARAKKKFLVAYTLLCVLATAFLALVD
                     RGMVMAGFVLLVLANVGMEGGIVFYNSFLNDIADNEHQGRVSAWGYATGYLGSIISLA
                     VALVLVKTDHITFVWPMVAVFFVMFSLPAFIFLPADQGKGQKLSKAARDGFATTWGVL
                     KRMWSDRDQRRFLLAYFLYEDGVNTVIVFSSIFASTTLGFTSEQLIGLYLVVQVTALA
                     GAFLMARPIDYWGPKKVVSLSLILWMVVTIVVYFIYSKAFFFVAASMAGLGLGTIQAA
                     TRAFFAQFIPPGDESKYFGVYSLVGKTSAVVGPLIFGTVSSMTNNQRPAVAAISVLFI
                     TGCIILQTVRGGKANVR"
     gene            1571..2086
                     /locus_tag="PKN85_06185"
     CDS             1571..2086
                     /locus_tag="PKN85_06185"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HNT44030.1"
                     /translation="MMDLSVFDTMKEDELRDYLTFLLWHYRLMDAFWFIYVSDTFGQE
                     QAERINEKVWSRVPEMGTRDLLQRFGITEKGLKGFTKVLKLFPWTILVEYKFEEKDSE
                     VIVTVPHCPTQEARLKRGLGEFVCKHMHHGEFESIARAVDPSIKVECLFAPPDEHPED
                     CFCKWRFTEAS"
     gene            2128..3237
                     /gene="ald"
                     /locus_tag="PKN85_06190"
     CDS             2128..3237
                     /gene="ald"
                     /locus_tag="PKN85_06190"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNT44031.1"
                     /translation="MIIGVPKEIKEGENRVSMTPAGVHTLVSAGHHILFEEGAGVGSG
                     LTDEEYAREGAEVVESKRTVYARSDMIVKVKEPLDTELSLLQEGQTLFTYLHLASSEK
                     LTNGLVKKKVTAVAYETMEDMFGRLPLLIPMSEVAGRISVQIAMRFLESDHGGRGVLL
                     SGVPGVPPADVVIIGCGVVGINAARIAAGLGAHVTVIDISQHRLRYVDDIMHGKVMTV
                     YSTSHAIRRAIQYADVLIGAVLVPGAKAPVLVSEQMVALMKPGSVIVDVSVDQGGCVE
                     TIRPTSHSEPSYKLYDVIHYAVPNMPASVPRTSTFALTNTTLPYIETIASMGVIKAAS
                     KDPLIASGINVKKGAITHPAVAEAFGMKWKKWNKV"
BASE COUNT          799 a          959 c          935 g          665 t
ORIGIN      
        1 gaccgtcgta gagggctact acacgataaa cgccgcctac aacctcgccg ccaggttagg
       61 cgtcccgatg cccatcacgg aggaactcta ccacatagtc tatgaaggca aggacatcac
      121 cgcgtccctg aaggacatca tcacgagaga attcaagaag gaagactatt agtcgcaggt
      181 cacaagtctc gggcctcagg tcagggcggc acactccaac tgcattttca ggttttgacg
      241 tgtgacatgc ggcctgcgac ctgcgacggg ggcttctatc ttacattcgc ctttccgccc
      301 cgcaccgttt ggaggatgat gcaacctgtt atgaagagca cggatatcgc cgccacggcc
      361 gggcgctggt tgtttgtcat cgaggacacg gtcccgaaga tgagggggcc gacgaccgcg
      421 gacgttttcc ccacaaggct gtacacgccg aagtacttgg attcgtctcc cggagggatg
      481 aactgggcga aaaaggcgcg tgtcgcggcc tggatcgtgc cgagaccgag gccggccatt
      541 gacgccgcca cgaagaagaa agccttgcta tagatgaaat agaccactat ggtgacaacc
      601 atccatagta tgagggacag cgagacgacc tttttcggcc cccagtagtc tatcggccgc
      661 gccatgagga acgcacccgc cagggccgtc acctggacga cgagatagag ccctataagc
      721 tgttccgacg tgaacccgag tgttgtggaa gcaaagatac tcgaaaagac gatcaccgtg
      781 ttgaccccgt cctcgtacag gaagtaggcc aagaggaacc tgcgctggtc cctgtccgac
      841 cacatccttt tcaagacccc ccatgtcgtg gcaaaaccat cccgggccgc ctttgacagc
      901 ttctgtccct ttccctggtc ggcaggaagg aagatgaagg ccgggaggga gaacatgaca
      961 aagaagaccg ccaccatggg ccagacaaag gtgatatggt ccgtcttcac cagaacgagc
     1021 gcgacggcga gtgagattat cgacccgaga taacccgtgg cgtatcccca ggccgatacc
     1081 ctcccctgat gttcgttgtc ggctatgtca ttgaggaagg aattgtagaa tactatcccg
     1141 ccctccatac ccacattggc aagtacgagc agaacgaacc ctgccatcac cattcccctg
     1201 tccaccaaag ccagaaacgc ggtagcgaga acgcacagca gcgtgtaggc gacgagaaac
     1261 ttcttcttcg cccttgcgta atccgagatc cctccgagga acggggagct caaggcaaca
     1321 aaggccatgc tcaccgatat ggcccttccc caccacaggt cacccaggtt cgcgtcgttc
     1381 ccgactacca ccgtcgtgta gtacacggga aatattaccg ccgcgatgac cgccgagtaa
     1441 cttgagttcg cgaagtcaaa gagacaccac gagaatattt ttctgtcaaa aaggctcgtc
     1501 ttcattgtct tttggtgttg aactaattta caataaacgt tgtcgtgatt cactaaaatt
     1561 gaggaggaac atgatggacc tttccgtttt tgacactatg aaggaagacg aactgcggga
     1621 ctacctgacc ttcctcctgt ggcattatcg tcttatggac gccttctggt tcatatatgt
     1681 cagtgatacc ttcggccagg aacaggctga gcgcattaac gaaaaggtct ggtcccgcgt
     1741 gcccgaaatg ggaacccgcg acctgctgca gcgcttcggc atcaccgaaa agggcctcaa
     1801 gggttttaca aaggtgctga agctctttcc gtggacgatc ctcgtcgagt acaagttcga
     1861 ggagaaggac tcagaggtca tcgttaccgt tccccactgc ccgacgcagg aggcgcgcct
     1921 gaaacggggc cttggggagt ttgtctgcaa gcacatgcat cacggggagt ttgagagcat
     1981 agcccgggcc gtggaccctt caataaaggt ggaatgcctc ttcgcccctc ccgacgagca
     2041 tccagaggat tgcttctgta aatggcggtt cacggaagcc tcgtgaatag agcctgtcgt
     2101 cgaaacgcta tctgatatgg aggaagaatg atcataggag taccgaaaga gatcaaggaa
     2161 ggcgagaaca gggtttccat gacaccggcg ggcgtccaca ccctcgtctc cgcgggtcat
     2221 cacatacttt tcgaagaggg agcgggggtg ggaagcggtc tcaccgacga ggaatacgcg
     2281 cgggaagggg ccgaggtcgt cgaatcaaag agaacggtgt atgccagatc ggatatgatc
     2341 gtgaaggtca aggagcctct cgacactgag ctttcccttc ttcaggaggg acaaacactc
     2401 ttcacctatc ttcacctggc ctcgtcggag aaactgacga atgggcttgt taagaaaaag
     2461 gtgacggccg tggcctacga gaccatggag gatatgttcg gccgtcttcc cctgctcatt
     2521 cccatgagcg aggtggcagg aaggatctcc gtccagatcg ccatgcgttt cctggaatcg
     2581 gaccacggcg gacggggcgt cctcctgagc ggcgttcccg gcgtaccccc ggcggatgtc
     2641 gttattatcg ggtgcggagt cgtgggtatc aatgcggccc gcatcgccgc gggtctcggc
     2701 gcccatgtga ccgtcatcga catctcccag caccggctga gatacgtgga cgatatcatg
     2761 cacggcaagg tcatgacggt ctattcgacg agccacgcga tccggcgggc gatccagtac
     2821 gccgatgtcc tcatcggcgc tgtcctcgta cccggggcga aggcccccgt tctcgtctcc
     2881 gaacagatgg tggccctcat gaaaccgggt tccgtcatcg tcgacgtctc cgtcgaccag
     2941 ggaggctgcg tggaaacgat acggcccacc tcccacagcg aacccagcta caagctctac
     3001 gacgtcatcc attacgccgt cccgaacatg cccgcgtcgg tcccgaggac gtccaccttt
     3061 gccctgacca ataccacact tccctacatc gagaccatag catccatggg cgtgataaag
     3121 gcagcatcca aagaccccct gatagcctcc ggcatcaatg tgaagaaggg cgcgataact
     3181 catcccgccg tagcggaagc cttcggaatg aaatggaaga agtggaataa ggtgtgaaaa
     3241 aacaggttcc atgttccatg ttccatgttc cagaagaaag atcgcgagag aacactcatt
     3301 gtcaatgaca atgaccgacg tcgcctcggt tctgttgtct ttagcgtgag acctgtga
//
LOCUS       DAOQTR010000219         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_32488_length_3355_cov_3.210909, whole genome shotgun sequence.
ACCESSION   DAOQTR010000219 DAOQTR010000000
VERSION     DAOQTR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32488_length_3355_cov_3.210909"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKN85_06195"
     CDS             complement(<1..348)
                     /locus_tag="PKN85_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44032.1"
                     /translation="MIADIVIIALIKALQCALMALPESARQGTGRLLGRMGFHLLRRR
                     RNIAISNIRRAFPDLSPNKAGDIALSCFQKLGTNVIELILVPFLPLREVPERFSLEPK
                     DIVETTLGAGRGVI"
     gene            complement(355..540)
                     /locus_tag="PKN85_06200"
     CDS             complement(355..540)
                     /locus_tag="PKN85_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNT44033.1"
                     /translation="MTISQDLLDILCCPKCKGDLVLTASGNGLTCAACRLLYPIRDGI
                     PVMLIEEALPIKPDDPK"
     gene            complement(559..2274)
                     /locus_tag="PKN85_06205"
     CDS             complement(559..2274)
                     /locus_tag="PKN85_06205"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNT44034.1"
                     /translation="MVETDTGAPKDNSQEDMQALYETSMKSLQEGNVLKGRVINIAGD
                     SVIVDVGLKSEGIVSLQEFPPKEGEAQPVSVDDEVEVMIVGREKESGLLRLSKRRVDE
                     IKTWEKIDKSLEDGTPLEGHILNEVKGGFIVDMGINAFLPLSQVDIKPVKNPSSFVGR
                     NLKFKVIKSNRRKGSIILSRRVLLEDERDKKRQEFWKTVKDGQIMYGFVRNITDYGAF
                     VDLGGVDGFLHINDITWGKITHPKEYLRIGDEVKVKIIAIDTEKGRVSIGMKQLKTDP
                     WLKIDEKYPVGSKVKGKVVGIVEYGAFVELEQGLEGLLHVSEMSWDKKFKNPAKLVNK
                     GDNLELVVLKIDLEKKRISLGLKQLAPDPWDELETNYPPGSIVKGKVKNFTEFGMFVG
                     IGNGIDGLVHMSELSWSRRKKAFQEQLKKGTTIDAFVLNIDKAQKKFSLSLKRLKEDP
                     WKGVGNRYSTGDVVEGHITSITDFGVFVEIEEGIEGLIHVSEMDELQGKQPSEVFTLD
                     DTIQAAILNIDEKDKRIALSTRVLRRKENRVNTETREESTGAFSTLGDILEPAMKKNS
                     GDSTD"
     gene            complement(2288..3109)
                     /gene="ispH"
                     /locus_tag="PKN85_06210"
     CDS             complement(2288..3109)
                     /gene="ispH"
                     /locus_tag="PKN85_06210"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNT44035.1"
                     /translation="MNVVKTKNIGFCFGVKRAIKMVLRAAEETGGKVFTLGPIIHNPQ
                     MVDLLKEKGVTPVDDVREVTDGTIVFRTHGIRKDEEEYALSTGLKIIDTTCPFVKRVR
                     KHAKYLERNGYQVVIVGDRNHPEVKSVLSYLDNDGIVLQEPTTLTAKKVGVVSQTTLD
                     KGTFVGIVKELIAGVEELRIYNTICESTQIRQNEAIELSGEVDMMLVVGGKNSSNCTK
                     LYKVVHKVQQNTHHIETVDDLRPEWFKNVSRVGLTGGASTPDLIIDIVEGRVKNF"
     gene            complement(3111..>3355)
                     /locus_tag="PKN85_06215"
     CDS             complement(3111..>3355)
                     /locus_tag="PKN85_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNT44036.1"
                     /translation="LDARPDERANRRYSELTTKGTGPALSAVREDMEKRDRNDSERAI
                     APLVVPPGAIVLDTTGTDVQGVLRMVLAHIEKEDRP"
BASE COUNT          592 a          988 c          891 g          884 t
ORIGIN      
        1 gatgacaccg cggcctgccc cgagggttgt ctcgacgatg tccttcggct cgagggagaa
       61 ccgctccggg acctcgcgga gagggagaaa ggggacgaga atgagctcta tcacgttggt
      121 gccgagcttc tggaaacagg agagggcgat gtctccggcc ttgttgggcg acagatcggg
      181 gaaggccctg cgtatgttgg agatggcaat gttcctgcgt cttcgcagga ggtggaatcc
      241 catcctgccg agcagcctgc cggtgccctg gcgggcgctc tcgggaaggg ccatgagggc
      301 gcactgcagt gccttgataa gagcgatgat tacgatatcc gcgatcacac cggcctattt
      361 cggatcgtcg ggtttgatcg gcagggcttc ctcgatgagc atgacaggta tgccgtcccg
      421 gatgggataa agaagacggc aggccgcgca cgtgagaccg ttcccggacg ccgtcaggac
      481 aaggtcgcct ttgcatttgg gacaacagag tatgtcgagt agatcctggc tgatggtcat
      541 tttcttcctt ccttagggtc aatccgtgct gtcgccactg ttcttcttca tggccggctc
      601 aagaatgtcg cccagggttg agaaagcgcc cgtgctctct tcgcgcgtct ccgtgttcac
      661 ccggttctct ttcctgcgaa gtaccctggt gctgagtgct atcctcttgt ccttttcgtc
      721 gatattgagg atggccgcct gtatcgtgtc atcgagggtg aagacctccg agggctgttt
      781 cccctggagc tcgtccattt cggagacgtg gatgaggccc tcgatcccct cctctatctc
      841 gacgaataca ccgaaatcag tgatgctcgt tatgtggccc tccaccacgt cacccgttga
      901 ataacggttt cccacgccct tccaggggtc ttctttcagg cgtttgagac ttaaggagaa
      961 cttcttctgt gccttgtcta tgttgagaac gaacgcgtct atggtggtgc ccttcttgag
     1021 ctgttcctgg aatgcctttt ttctcctgga ccaggaaagt tccgacatgt ggacaagtcc
     1081 gtcgatgccg ttgcctatgc cgacgaacat accgaactcg gtgaagttct tgaccttgcc
     1141 cttcacgatg gagccaggcg gatagttcgt ctcaagctca tcccaggggt cgggagcgag
     1201 ctgtttaagg cccagggaga tgcgtttctt ctcgaggtcg atcttgagga cgacgagttc
     1261 caggttgtca cccttgttga cgagctttgc agggttcttg aacttcttgt cccagctcat
     1321 ctcgctgacg tggagcagtc cctcgagccc ctgctcgagt tccacgaatg ccccgtactc
     1381 cacgatcccc acgaccttgc ccttcacctt gcttccgacg gggtacttct cgtctatctt
     1441 cagccacggg tcggtcttga gctgtttcat gcctatggag acacgtccct tctcggtgtc
     1501 gatggctatg atcttgacct tcacctcgtc cccgatcctg aggtattcct tggggtgggt
     1561 gatcttgccc catgttatgt cgtttatgtg cagaaaacca tcgacaccgc cgaggtcgac
     1621 aaaggccccg tagtcggtga tgttccggac gaaaccatac atgatctgtc cgtccttgac
     1681 ggtcttccag aattcctgtc tcttcttgtc tctttcgtct tcgaggagta cccgtctcga
     1741 gaggatgatg ctgccctttc tccggttgga cttgatgacc ttgaacttca ggttccttcc
     1801 cacgaatgat gaagggttct tgacgggttt gatatccacc tgcgagagag ggaggaaggc
     1861 gttgatcccc atatcgacga tgaacccgcc cttgacctcg ttcaggatat gaccttccag
     1921 gggcgtgccg tcttcgagtg acttgtcgat cttttcccag gtcttgatct cgtcgaccct
     1981 cctcttcgag agcctcagga ggcccgactc cttctccctt ccgacgatca tcacttccac
     2041 ttcgtcatcg acgctgacag gctgcgcctc gccttctttc ggggggaatt cctgcaggga
     2101 gactatgccc tcggacttca aacccacgtc gacaatgacc gaatcaccgg cgatgttgat
     2161 caccctgcct ttcaggacat tgccttcctg caggctcttc atcgacgttt cgtacagggc
     2221 ctgcatgtcc tcctgtgagt tgtctttcgg tgccccggta tctgtctcca ccatgttcct
     2281 gtgcccccta aaagtttttt acgcgcccct caacgatatc tatgataagg tcgggagtcg
     2341 atgcgcctcc cgtcaaaccc acgcgggata cgttcttaaa ccattccggc ctgagatcat
     2401 caacggtttc gatatgatgg gtgttttgtt gtactttgtg aacgaccttg tagagctttg
     2461 tgcagttgga gctgttcttt ccccccacca ccaacatcat atcgacttcg cccgataact
     2521 ctatggcctc attctgtcgt atctgggtgc tttcgcaaat tgtattgtag atcctcagct
     2581 cctccacgcc tgcgataagc tccttgacga tgccgacaaa ggtgccttta tcgagggtgg
     2641 tctggcttac aactcccacc ttcttggcgg tcaatgttgt tggttcctgt agtacaatac
     2701 catcgttatc caaataactc aagacacttt tgacctccgg atggttcctg tcgcccacga
     2761 tcacgacctg gtagccgttc ctctcgaggt actttgcgtg ttttcggacg cgcttcacga
     2821 agggacaggt cgtgtcgatg atcttgaggc ccgtcgacag cgcatattcc tcttcatcct
     2881 tccgtatccc gtgggtcctg aagacgatgg tgccgtccgt gacctcccgg acatcatcga
     2941 caggtgtgac gcccttctcc ttcaggaggt ccaccatctg cggattgtgg atgattggcc
     3001 ccagggtgaa gaccttcccg cccgtctcct cggcggccct gaggaccatc ttgatggcgc
     3061 gcttgacccc gaagcagaag ccgatattct tcgtcttgac cacgttcatt ctatggacga
     3121 tcctccttct cgatgtgtgc aaggaccatc ctcaagacgc cctgcacgtc cgttcccgtt
     3181 gtatcgagga ctatagcacc cgggggaacc acgagagggg ctatggcccg ttcggagtcg
     3241 ttcctgtccc gcttctccat atcctccctc actgcggaca acgccggacc ggtgcccttt
     3301 gtcgtcaatt cgctgtacct gcggttcgcc ctttcgtcgg gacgggcgtc gagat
//
LOCUS       DAOQTR010000220         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_32619_length_3345_cov_3.364134, whole genome shotgun sequence.
ACCESSION   DAOQTR010000220 DAOQTR010000000
VERSION     DAOQTR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32619_length_3345_cov_3.364134"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PKN85_06220"
     CDS             <1..941
                     /locus_tag="PKN85_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNT44037.1"
                     /translation="IEPMAEDAAFRDRFNAVKKENKLRLCRAMERVFNFTLETDLLLD
                     CQVKRFHEYKRQLLNVFHVITLYNRLKEGRVPEGFVPRVVLFSGKSAPGYLMCKLIIK
                     LIHNVSEIVAAHPLVKDKLQVVFVPNYGVTIAEVTIPAADLSEQISTAGYEASGTGNM
                     KFALNGALTIGTYDGANIEIREEVGEENFFLFGHRAEEIFELRKQYDPKKYIEENKEL
                     AQVMHQLQSGFFSPEEPALFQPIVQALMDGDRYCVLADYSLYVACQEEVARAYSDRDA
                     WTRKAILNTARSGKFSSDRAIHEYARDIWSISPVNR"
     gene            1155..3083
                     /locus_tag="PKN85_06225"
     CDS             1155..3083
                     /locus_tag="PKN85_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT44038.1"
                     /translation="MERYPHIFQQGTIGNLTVKNRIKYAATETNFPYGDGYVSDREVV
                     YMEAQAKGGAGIVTTQGAYPDKKGEGKGFKGMMSINDDKYIPGLKRIADVIRANGALS
                     SIQILHCGREGGIELDYCLMPSIVPQKLSYFKPPREITKEEIKEAVKDHVAAARRAKE
                     AGFDMIELSGIVGYLISTFISRYTNKRTDEYGGDLRQRCRLMVEVIEGVKAEVNDMPV
                     GIRLCGHELLDDRGGNTFEESVESFRIAEDAGADYLSVTVGWHESSQSVITRDVPMGH
                     WLYIAEAVKKAVGVPVMMAFRQFLPHIPEKAISGGQIDFVEMCRPMIADPEFPKKVSE
                     GREGEIIPCIACNVCFSRLYYHQPIMCSVRPSLGQEGEKGWGYYGFEPTEKRKKVLVV
                     GGGPAGLQCAEVAARKGHDVTLFEKNDRLGGNILIASRVDAGAAELMRPIAALEQLCR
                     KAGVDIRLGAACDPATVGSEAPDVLVVASGPSIKGVSKGVLTPHGVMAAGEKVGPRVV
                     IMGAGGVGLGVAVYLLRSGEYEITVIDEAGKPGRDVNPFYFWQYMALMRKRKVNFMMR
                     TVVNVVEDDRVRVTGPSGEGVVEADSVITSLMAPEEGVWKDTAGSLAKEVYFIGDAKK
                     PRRLLNAIRDGYRLGMVL"
     gene            3083..3277
                     /locus_tag="PKN85_06230"
     CDS             3083..3277
                     /locus_tag="PKN85_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT44039.1"
                     /translation="MIPVVDKETCTGCATCWEVCPPGAILFRDGKACIDEDLCEECGF
                     CAAECPVKAIEIPFPNSKTA"
BASE COUNT          743 a          906 c         1062 g          634 t
ORIGIN      
        1 agatcgagcc catggcggag gacgccgctt tcagggaccg tttcaacgcg gtcaagaaag
       61 agaacaagct tcgcctctgc agggcgatgg aaagggtctt caacttcacc cttgaaacgg
      121 atcttctcct cgattgccag gtgaagagat tccacgaata caagaggcag ctcctcaacg
      181 tttttcacgt catcacgctt tacaaccgtc tcaaggaagg acgggtgccg gagggtttcg
      241 tgccccgcgt ggtcctcttc tcgggcaagt cggcgccggg gtatctcatg tgcaagctca
      301 tcatcaagct catacacaac gtctccgaga tagtcgccgc ccatccgctg gtaaaggaca
      361 agctccaggt ggtcttcgtc ccgaactacg gtgtaacgat cgccgaggtc accataccgg
      421 cggcggacct gtcggagcag atatcgacgg ccggctacga ggcgtcgggc acgggaaaca
      481 tgaagttcgc cctcaacgga gcactcacga taggaactta cgacggcgcg aacatagaga
      541 tacgggaaga ggtgggggag gagaacttct ttctcttcgg acacagggcc gaggagatct
      601 tcgaactgag gaagcagtat gacccgaaga aatacattga agagaacaaa gagcttgccc
      661 aggtgatgca ccaactccaa tcggggttct tctcaccgga agagccggcc ctgttccaac
      721 ccatcgtgca ggccctgatg gacggcgatc gctactgcgt cctggccgat tattccctgt
      781 atgtagcgtg tcaggaggaa gtggcgcggg cctacagcga cagggacgcg tggacgcgca
      841 aggcgatcct taacacggca aggtcgggca agttctcaag cgaccgcgca atccacgagt
      901 acgcccggga catatggagc atctcaccgg taaaccggtg aagaaggttc aagaggtaaa
      961 gaaggttcaa gagttccaga gttcaagagt aaaagacaaa aagggcaccg gcgccttcgg
     1021 ccggtgtgcg agttgcgttc ttccatggtc tttcccttga acccttgaac ctttttttcc
     1081 gtcttgactc gtttctggtg gtgtgttact caattcactg tttcttcact tctgaacata
     1141 acagggtggc gatcatggaa cgatatccgc acatctttca acagggcacc atcggcaacc
     1201 tgactgtcaa gaacaggatc aagtacgcgg cgacggagac gaactttcct tacggcgacg
     1261 gctatgtgag cgaccgcgag gtggtctaca tggaggccca ggccaagggc ggggcaggca
     1321 tagtgacgac ccagggagcg taccccgaca agaaggggga gggcaagggc ttcaagggga
     1381 tgatgtcgat caatgacgac aagtacatcc ccggcctcaa gcggatagcc gatgtgatcc
     1441 gtgccaacgg ggccctttcg agcatccaga tactccattg cggcagggag ggcgggatcg
     1501 agctcgacta ctgcctcatg ccgtccattg tcccccagaa gctctcctac ttcaagcccc
     1561 cccgcgagat cacgaaggaa gagataaaag aggccgtgaa ggaccacgtg gcggctgcgc
     1621 ggcgcgcgaa agaggccggc tttgacatga tagagctttc cggcatcgtc ggttacctca
     1681 tctccacgtt tatatcgcgt tacacgaaca agaggaccga cgaatacggc ggggatctga
     1741 gacagcggtg ccggctgatg gtggaggtca tcgagggcgt gaaggccgag gtgaacgata
     1801 tgcccgtggg catccggctc tgcggccacg agcttctcga cgaccggggt ggcaacacct
     1861 tcgaggagag cgtcgaaagc ttcaggatag ccgaggacgc cggggcggat tacctgagcg
     1921 tcaccgtggg gtggcacgaa tcatcccagt cggtcatcac gcgggacgtt cccatgggtc
     1981 actggctcta catagcggag gcggtcaaga aagccgtcgg cgttcccgtc atgatggcct
     2041 ttcggcagtt cctccctcat atccccgaga aggcgatatc cggggggcag atagacttcg
     2101 tggagatgtg ccggcccatg atcgccgacc ccgaattccc gaagaaggtc agcgagggca
     2161 gggaagggga gataatcccc tgcatcgcct gcaacgtttg cttctcgcgg ctttactacc
     2221 accagcccat catgtgctcc gtccgtccca gcctgggaca ggagggcgaa aaagggtggg
     2281 gatactatgg gtttgaaccg acggagaaga gaaagaaggt gctcgtcgtc ggcggcgggc
     2341 ccgcgggcct ccagtgcgcc gaggtggccg cgcgcaaggg ccacgatgtc acgctcttcg
     2401 agaagaacga caggctgggc ggtaacatcc ttatagcctc cagggttgat gcaggcgccg
     2461 cagagctgat gcggccgata gcggccctcg agcagctctg ccgcaaggcc ggggttgaca
     2521 tcaggctcgg cgccgcgtgc gatccggcca ccgtcggctc ggaggcgccc gatgttctcg
     2581 tggtggcgtc cgggccctcc ataaagggtg tctccaaggg tgttttgacg ccgcacggtg
     2641 tgatggcggc gggggagaag gttggaccga gggtcgtcat catgggtgcg ggcggtgtag
     2701 ggctgggtgt tgccgtttac ctccttcgca gcggcgagta tgagatcacc gtcatagacg
     2761 aagcgggaaa gcccggcagg gacgtcaatc ctttctattt ctggcagtac atggccctca
     2821 tgagaaagcg aaaggtcaat ttcatgatgc gcactgtcgt caatgtggtg gaggacgacc
     2881 gggtgcgtgt cacggggccc tcgggcgagg gggttgtcga agccgacagc gtcataacgt
     2941 cgctcatggc cccggaagaa ggtgtatgga aagataccgc gggatcgctt gcgaaagagg
     3001 tgtatttcat cggagacgcg aagaaaccga ggcgccttct gaacgcgatc cgtgacggat
     3061 accgcttagg gatggtgctc tgatgatccc cgttgtcgac aaggaaacat gcacaggatg
     3121 cgcgacatgc tgggaggtat gtccccccgg tgccatactg ttcagggatg gtaaggcctg
     3181 catcgacgaa gacctgtgcg aggaatgcgg cttctgcgcc gccgaatgcc cggtaaaggc
     3241 gatagagata ccctttccaa acagtaagac ggcttgaatc gcttgaatgg cttgagtcgc
     3301 ttgacagcct gttgataaac tctgtctcac ggcatgatcc cgttc
//
LOCUS       DAOQTR010000221         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_32694_length_3338_cov_3.796528, whole genome shotgun sequence.
ACCESSION   DAOQTR010000221 DAOQTR010000000
VERSION     DAOQTR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32694_length_3338_cov_3.796528"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKN85_06235"
     CDS             complement(<1..399)
                     /locus_tag="PKN85_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44040.1"
                     /translation="MNEKKTVVVISQDVVLSGIIDRILSRDYGVFLFRNIQSAIDYIY
                     NSPPNLIILDTSEEDIYSMDVINNLKSDPIFYQLPVLVVIDDIPSVPVWKDLFVEDYL
                     KREDIEQEGVTRVALAILRSERIVEVNPLTK"
     gene            complement(396..1232)
                     /locus_tag="PKN85_06240"
     CDS             complement(396..1232)
                     /locus_tag="PKN85_06240"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT44041.1"
                     /translation="MNSMDVRAVREKIEHINALPTIPKVLNRLLALIENPRVSLNEIS
                     NFISSDPALTTKVLRMVNSPVYGFPGRISSVNQAVILLGLTVVKGLLLGVSVFELMQK
                     TMIGLWEHSIGTALFSRIIAVRAGHKEPDEVSVDGLLHDIGKVFLVLQFPSEYQRALT
                     QTKEDGIIIHEAEKNHFSTTHASVGSWMARKWRFPAKLIDVIEYHHKPHLTKSTAVES
                     AIVHIADILVRARGFGFPGDDLMTPVQPEAWDLLKLTERDILEILEEAEDSLQATEDL
                     TL"
     gene            complement(1225..1521)
                     /locus_tag="PKN85_06245"
     CDS             complement(1225..1521)
                     /locus_tag="PKN85_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44042.1"
                     /translation="MPKIAPGSLEPGMKTAKPVVNTSGMVLLGENTELTPELIDRIRN
                     MEVDGVWIQGLSKPSVPMEEVVAELDRRFRLVGGQPHMDIIRKAVQRHIEGLYE"
     gene            complement(1605..2744)
                     /locus_tag="PKN85_06250"
     CDS             complement(1605..2744)
                     /locus_tag="PKN85_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT44043.1"
                     /translation="MNISRRASEISPFYVMELLEQARLMEAAGHSVIHMEVGEPGFET
                     PGTIRQEAEKALAEGKTFYTHSLGIPELRQAVSRHYRDRYDLDISPERVIITNGSSGA
                     FILLFAALLERGRTLDIADPGYPCYRNIALLTDCAVRALPVSAETNYEVLPEQLGGSP
                     APDVLVIANPSNPTGTIYRAGALNAIHKRLSSRGGVLVVDEIYSGLVYDAPFSSSAAI
                     SEDVIVVNGFSKAFAMTGWRLGWMVVPPGLVRPIQKLAQNLFIAPPSISQYAALAAFD
                     DADGLARMCGLYRERRDFLLPRLRGLGFDIPVTPDGAFYIYAGIERFGMDSMLFVERA
                     LAEAKVAITPGYDFGRFGAASHVRFSYADRLENLVEGCDRLERWL"
     gene            2920..>3338
                     /locus_tag="PKN85_06255"
     CDS             2920..>3338
                     /locus_tag="PKN85_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNT44044.1"
                     /translation="MTKTQVVRYARENGVTFIKLWFTDLLGFAKSFTITIDELENALE
                     EGMGFDGSSIQGFARIDESDMIAKPDVSTFTILPWRPQGEASVARMFCDIFEPDGTPY
                     KGDPRYVLKRNLKAAQEMGFDLFVGPELEYFYFKSDSG"
BASE COUNT          642 a          929 c         1100 g          667 t
ORIGIN      
        1 cttggtaagg gggttcacct ccacgatgcg ctccgagcgc agtatcgcca gggcaacgcg
       61 ggtgacgcct tcctgctcga tatcctccct cttcaggtaa tcttcgacga aaaggtcctt
      121 ccagacggga acgctcggga tgtcgtcgat gacgacgaga acgggcaact ggtagaagat
      181 ggggtcgctc ttgaggttgt tgatgacgtc catggagtag atgtcctctt cggaggtgtc
      241 gaggatgatg aggttcggcg gcgaattgta gatgtagtcg atggcggact ggatgttcct
      301 gaagaggaag acgccgtagt cccgggaaag gatgcggtct attatgcccg agaggacaac
      361 gtcctgcgat atgacgacga cggtcttctt ctcgttcata gggtcaggtc ttccgtggcc
      421 tgcagggagt cctcggcttc ctcgagtatc tcgaggatgt ccctctcggt gagcttgagc
      481 aggtcccatg cctcgggctg gacgggcgtc atgaggtcgt cgccgggaaa gccgaaaccc
      541 cgggccctca cgaggatgtc ggcaatatgg acgatggcgg actcgacggc ggtgctcttc
      601 gtcaggtgtg gcttgtgatg gtactctatg acgtcgatga gctttgccgg gaagcgccat
      661 ttcctggcca tccagctgcc cacgctggcg tgtgtcgtgc tgaaatggtt cttttcggcc
      721 tcgtggatga tgatgccgtc ctctttcgtc tgcgtcaggg ccctctggta ctcgctgggg
      781 aactggagca cgaggaagac cttgccgata tcgtggagaa ggccgtcgac ggagacctcg
      841 tcgggttcct tgtggcccgc ccgcacggca attatgcgtg agaacagggc cgtccctatg
      901 gaatgctccc agaggccgat catggtcttc tgcatgagct cgaagacgga aacgccaagg
      961 aggaggcctt tcacgacggt gagtccgaga agtatgacgg cctggttcac ggaagagatc
     1021 cttccgggga agccgtatac gggagagttg accatcctga gcaccttcgt ggtgagggcg
     1081 gggtcggagg agatgaagtt cgagatctcg tttagggaga cccgggggtt ctcgatgagc
     1141 gcgaggagcc tgttgaggac cttgggaatg gtggggagag cgtttatgtg ttcgatcttt
     1201 tccctgaccg ccctgacgtc catgctattc atagaggccc tcgatgtgcc tttggaccgc
     1261 cttccttatg atatccatgt ggggctggcc gccgacgagg cggaagcgcc tgtcaagttc
     1321 cgcgacgacc tcctccatcg ggaccgacgg cttcgacagg ccctggatcc acaccccgtc
     1381 cacctccatg ttccttatcc tgtcgatgag ctccggggtg agctccgtat tctccccgag
     1441 aaggaccatg ccgcttgtgt tcacgacggg cttcgcggtc ttcattcccg gttcgagact
     1501 gccgggggcg atcttcggca ccacaatcct ccttggaata ctatcggcca aaagccggaa
     1561 aacctaatac ccggtcgccc gacctctcgg ggcgctcgtc ccggttacag ccacctttcc
     1621 agcctgtcgc agccttccac gaggttctcg agcctgtccg cgtaagagaa acggacgtgg
     1681 gaagcggccc cgaagcggcc gaaatcatac cccggggtga tggccacctt tgcctcggca
     1741 agtgcccttt ccacgaagag catgctgtcc atgccgaagc gctcgatgcc ggcgtaaatg
     1801 taaaaggccc cgtcgggcgt gacgggaatg tcgaatccaa ggccccgaag gcgcggcagg
     1861 aggaagtccc tcctctccct gtacagaccg cacatgcggg cgagaccgtc ggcatcgtca
     1921 aaggccgcga gggccgcgta ctgcgagatg gacggcggcg cgatgaaaag gttctgcgcc
     1981 agcttctgga tggggcggac gaggcccggg gggaccacca tccagccaag gcgccaccca
     2041 gtcatggcga aggccttgga gaagccgttc acgacgatga cgtcctcgga gattgccgcg
     2101 ctggaggaga acggtgcatc ataaacgagg ccggagtata tctcgtcgac gacaaggacg
     2161 cccccgcgcg aagagagcct cttgtgaatg gcgttcaggg cgccggcgcg gtagatcgtt
     2221 cccgtcgggt tcgaggggtt ggcgatgacg agcacatcgg gtgccggcga gccgccaagc
     2281 tgctcgggaa ggacctcgta attggtctcg gccgaaacag ggagggcacg cacggcgcaa
     2341 tcggtgagaa gggcgatgtt ccggtagcag ggatacccgg ggtcggctat gtcaagggtc
     2401 cggccccgct cgaggagtgc cgcgaagagg aggatgaatg cgcccgagga gccgttggtg
     2461 atgatcacgc gctcgggaga tatgtcgagg tcgtagcggt cccggtagtg gcgggagact
     2521 gcttgcctca gctcggggat gccgaggctg tgggtgtaga aagtcttgcc ctcggcgagg
     2581 gccttctcgg cctcctgtct tatcgtgccc ggcgtctcga agcccggttc gccgacctcc
     2641 atgtggatga cgctgtgccc ggcggcctcc atcaaccggg cctgctcgag gagttccatg
     2701 acatagaagg gggatatctc gctggccctt ctggagatgt tcatagtcgg ttcctcatct
     2761 gttcccccgg gatgtgccgg atcgacgcta acgaactgta ccagaatgga gcctcgcctg
     2821 tcaacgggat gcgcgtcctg gggccaagga aaaacattga ctttgaaggg acatctcttc
     2881 ataatggagg aaatcgggga acatgacgga gggcgatcga tgacgaagac acaggtggtg
     2941 agatatgccc gggagaacgg cgtcaccttc ataaaactgt ggttcacgga cctgctcggg
     3001 ttcgcgaaga gcttcaccat caccatcgac gagcttgaga acgcccttga agagggaatg
     3061 ggtttcgacg ggtcctcgat ccagggtttt gcccgcatag acgagagcga catgatcgcg
     3121 aagcccgacg tgtcgacctt cacgatactg ccctggaggc cccaggggga ggcctcggtg
     3181 gcgcgcatgt tctgcgacat cttcgagccc gacgggacac cctataaggg agacccgcgg
     3241 tacgtgctga agaggaacct caaagccgcc caggagatgg ggttcgatct cttcgtgggc
     3301 cccgaactgg agtatttcta tttcaagtcc gattcggg
//
LOCUS       DAOQTR010000222         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_32966_length_3319_cov_3.775429, whole genome shotgun sequence.
ACCESSION   DAOQTR010000222 DAOQTR010000000
VERSION     DAOQTR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32966_length_3319_cov_3.775429"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PKN85_06260"
     CDS             <1..951
                     /locus_tag="PKN85_06260"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNT44045.1"
                     /translation="EGKDPATPACVIRSGTLPAQKVVSATLATISEETRKMQLTPPGV
                     IVVGDVIHLREKLNWFEQKPLFGKKVVVTRSAHQSRRFGELLADRGAEPVYLPTIDIE
                     PIVPNSRLRSAIDRISSYDAIIFTSVNGASIFFAHLRGAGRDARALSGLAIIAIGAAT
                     AAHLGVYGIQADLVPEKFTSEGVVSVLEAYGVSGRNFLIPRAEEARDVITRYIRANGG
                     SCSIVPIYRATIPAKAASLPDDLDIVTFTSSSTASNFIMLYGKEILKNRVVASIGPVT
                     TATLTKSGINVDIEAQQSDIPGLLNAIEAYFQPPAKNGKA"
     gene            complement(965..1144)
                     /locus_tag="PKN85_06265"
     CDS             complement(965..1144)
                     /locus_tag="PKN85_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44046.1"
                     /translation="MEGNRKENVTFLGLQRVPRGEALVLVNNESGSTVTYDPARHNII
                     NMDGYIRSVLASRRR"
     gene            complement(1259..1447)
                     /locus_tag="PKN85_06270"
     CDS             complement(1259..1447)
                     /locus_tag="PKN85_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44047.1"
                     /translation="MLMKLSLRKGGTKWVDHNEARMLQRQGQVICVLAIKNEELAEFP
                     PFCSMRNDYCSSCISLPD"
     gene            complement(1943..2740)
                     /locus_tag="PKN85_06275"
     CDS             complement(1943..2740)
                     /locus_tag="PKN85_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT44048.1"
                     /translation="MKKDSDEIRALIVDDERLARENLKELLRDQWDVRVVGEARNADE
                     AERMIAALNPNLVFLDIQMPGGSGFDLLARLEDTPSIIFVTAFDQYAIRAFEVNALDY
                     LLKPIEPERLRNALRRAAFRLPSFDPAGEIFSAADQVFLNTGKKASFVSASNIVAIFS
                     ERNYTNVHNIQGERFMVRSSLRDWERRLPPDMFVLLDRSTIINRNHVELWTLHARKAE
                     LKMTGLAEPIELGRAAYTRFKELIVAPKAKGQGAGKGTRGEDEGETA"
     gene            complement(2724..>3319)
                     /locus_tag="PKN85_06280"
     CDS             complement(2724..>3319)
                     /locus_tag="PKN85_06280"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNT44049.1"
                     /translation="TLRYQINPHFLFNVLNSLDALSKTDPERISELVRQLSRYLRSTL
                     SSRESGLTSLEKEMSTIESYLSLEKVRFEEDLVVSVSMPEEIGDALVPELLIQPVVEN
                     AVKHGMKTSSLPLRVDVTCRAAGEHVMIEVANTGKWIARDTAADAGKGGVGLGNIRTR
                     LELTYGDRYGLEIGGSDDRVVVTIKIPLRGDKNEKGL"
BASE COUNT          671 a          988 c          925 g          735 t
ORIGIN      
        1 gaaggcaagg acccggccac cccggcttgc gtcatccggt cgggtaccct gccggctcag
       61 aaggtcgttt cggcaacgct tgccacgatc tccgaggaga cgcgcaagat gcagctcact
      121 ccaccggggg ttattgtggt cggcgacgtt atccacctga gggaaaagct gaactggttc
      181 gagcagaaac ctctcttcgg gaaaaaggtg gtggtgacgc ggtcggcaca ccagtcccgc
      241 aggttcggtg aactcctcgc ggaccggggt gccgaacccg tctatctccc gaccatcgat
      301 atcgagccca tcgtccccaa ctcccggctt cgctccgcga tagacaggat atcctcatat
      361 gacgccatca tcttcacgag cgtcaatggg gcctccatat tcttcgcgca tctcagggga
      421 gcggggaggg acgcccgggc cctgagcggc ctcgccatta tcgccatagg agccgcaacg
      481 gccgcgcatc ttggcgtcta cggcatacag gctgaccttg ttccggaaaa gttcacgtcc
      541 gagggagttg tctccgtcct tgaggcctat ggcgtttcgg gaaggaattt tctcatcccc
      601 cgtgctgaag aagcccgcga tgtgatcact cgatacataa gggcaaatgg aggaagttgc
      661 agcatcgtgc ccatttacag ggcaacgatc ccggcgaagg ccgcatccct acccgacgat
      721 ctcgacatcg tcactttcac gagttcttcc acggcaagca atttcattat gctctacgga
      781 aaggagatct tgaagaacag ggtcgtcgcc tcaataggcc ccgttacgac ggcaaccttg
      841 acaaagtcgg gtatcaatgt cgatatcgaa gcccagcaat ccgatatccc cggattgctg
      901 aacgctatcg aggcctattt ccagcctccc gcaaaaaacg gtaaggctta aggcaagcct
      961 ctcgtcacct gcgccttgac gccaggacgg aacggatgta tccgtccatg ttgataatgt
     1021 tgtgcctggc ggggtcatat gtgacggtgc tcccgctttc gttgttcacc agaacgagtg
     1081 cttctccccg cggcaccctc tgaagcccga ggaatgtgac gttctccttt cgattacctt
     1141 ccatcgccgt cctcccttat ctggggacct gcggttccgt cgcgggcact cgaacatgtc
     1201 gtctccgccg cataacagtt cagaaagccg caggccacat ccgggctacg cagaccctct
     1261 agtcgggcaa cgagatacag gaactgcagt agtcgttcct catgctgcag aaaggcggaa
     1321 actcggcaag ctcttcgttc tttatggcga gaacgcagat gacctgtccc tgcctctgga
     1381 gcatcctggc ttcgttgtgg tccacccatt tcgtcccgcc cttccttaaa gacaatttca
     1441 tgagcatatc gtcctccctc gttgttgtaa tgatagggta tcctatccag tctgccggag
     1501 ggtccgagag gctatgtgcc gaatcgaccc gccggagttg ccgacaagcc atccagaggt
     1561 cattcaaggt ctccgggact gtgttagcgg gtgactgctt ttggggaagc cggggcactc
     1621 actccccccc gcaggtgaat gtgccccgac ttggtgggat gatgaaacat tggggtttgc
     1681 ggatacaatt ctaccggatt atcggggccc gcgtaagaca gagggacaga ccagacctga
     1741 ttagtgacga accacggaac agtcggtgat agcgaggaca aggaggccgg gacttgtgca
     1801 gacacgatgg tgttggcatg tcccggcctg ttggaatggt gcgactacag gggtttgtgg
     1861 aaatggattt tatatgacaa gaaaggaggg tgccagtatt attgtacgat cgtgcccgat
     1921 gaagtgctca aacagcaatc ctttacgccg tctccccttc atcttctccc ctcgtaccct
     1981 tcccggctcc ctgacctttc gctttcgggg cgacgatcag ttccttgaac cgggtgtagg
     2041 ccgccctgcc cagctcgatc ggctccgcca ggcccgtcat cttgagctcc gctttcctgg
     2101 cgtgaagggt ccacagctcg acgtggttcc tgttgatgat cgttgaacga tcgaggagga
     2161 cgaacatgtc cggaggcagg cgcctctccc agtctctcag cgacgaccgg accatgaagc
     2221 gctctccctg gatgttgtgg acattggtat agttcctctc ggagaaaatg gccacgatat
     2281 tgctcgcgga cacaaaagac gccttctttc cggtattgag gaagacctga tcggcagcgg
     2341 agaagatctc tcccgcggga tcgaaggaag gcagacggaa cgccgccctt ctcaacgcgt
     2401 tcctgagacg ttccggttcg atgggtttca ggaggtaatc caacgcgttg acctcgaacg
     2461 cccgtatggc gtactggtcg aaggccgtaa cgaagatgat ggaaggcgta tcttccaggc
     2521 gcgcgaggag atcgaacccc gatcctcccg gcatctggat gtccaggaat accaggttgg
     2581 gattgagggc ggcgatcatc ctttccgcct cgtccgcgtt ccttgcctcg ccgaccaccc
     2641 tgacgtccca ctggtctctc agcagttcct tcaggttctc ccgtgcaagc cgttcgtcgt
     2701 cgacgatgag tgcccggatc tcgtcagagt cctttttcat tcttgtctcc tcttagaggg
     2761 attttgatgg tgaccaccac cctgtcgtct gacccgccga tctcaaggcc atatcggtca
     2821 ccataggtaa gctcaagccg tgtgcgtatg ttcccaagcc cgacaccgcc ctttcctgca
     2881 tccgctgcgg tatcccgggc gatccacttc cccgtgttcg cgacctcgat catcacgtgc
     2941 tcgcctgccg ccctgcacgt cacatcaacc cgaagaggaa gggacgatgt cttcatcccg
     3001 tgcttcacgg cattttccac gacgggctgt atgagcagtt cgggaaccag ggcgtcccct
     3061 atctcctcgg gcatgctgac agagacgacc agatcctcct cgaaccggac cttctccagg
     3121 ctgagatagc tttcgatggt gctcatctcc ttttcgaggg atgtaagtcc cgactcccgc
     3181 gacgagagag tggagcgcag gtaccgggag agctgccgca caagttcgct gatgcgttcc
     3241 gggtctgtct tcgaaagggc atccagggaa ttgaggacat tgaaaaggaa atggggattg
     3301 atctggtacc tcaacgtct
//
LOCUS       DAOQTR010000223         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_33045_length_3314_cov_3.932495, whole genome shotgun sequence.
ACCESSION   DAOQTR010000223 DAOQTR010000000
VERSION     DAOQTR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33045_length_3314_cov_3.932495"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKN85_06285"
     CDS             complement(<1..571)
                     /locus_tag="PKN85_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44050.1"
                     /translation="MTTTIKIGRLVRCAIVKDLADRDVYLVIVTGTDLLAYLPKRFAG
                     REYRQGEETVAAVFMMGKGKIFLSQRSPQYFRRITEEALSPIIRDGRVRVRRAASVIN
                     GPFAKVSVEGLGDVDPLSASLPYLEGVKAYTADTITIVRYSTDMEKYIVNSLVPAPPD
                     KVVEVVYSRGLREAIVRVDPRYCGFFVGKG"
     gene            817..1008
                     /locus_tag="PKN85_06290"
     CDS             817..1008
                     /locus_tag="PKN85_06290"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HNT44051.1"
                     /translation="MECTMEKNKAACACTYDPCPRKGKCCECVAYHRARKQLPGCFFS
                     KEGEASYDRSYRRFVMENS"
     gene            complement(1153..1644)
                     /locus_tag="PKN85_06295"
     CDS             complement(1153..1644)
                     /locus_tag="PKN85_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44052.1"
                     /translation="MGDYDDERPDWRELDRRRDRSTFYGRQEKGAGKKKEAPKDRWQQ
                     GRVKDALDRLFKGDKGTPEHDKLFARLHNAYGSEAFPKQAERYMAKYGLPDDGPTLIL
                     FLDLKDADVCNSTLDKLRELYASLPPRQKEDARRKISIAAMAHKIKEVRIKAQEVIEE
                     LEE"
     gene            1880..2185
                     /locus_tag="PKN85_06300"
     CDS             1880..2185
                     /locus_tag="PKN85_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44053.1"
                     /translation="MFIWLFHRISGVALIVLFGIKILTSYFLFTEDKKPDWALSLHRQ
                     PVLDVLILLLFTFHSIYGIRTIVMDLGYRNEKRLFVASNVVASVISAVLLYLYLVIS"
     gene            2182..>3314
                     /locus_tag="PKN85_06305"
     CDS             2182..>3314
                     /locus_tag="PKN85_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT44054.1"
                     /translation="MTGLSHDVLIVGGGLAGLRAAVGLCDKYDVALLSKVHPIRSHSI
                     AAQGGINAALANNPDSRDDTWEKHAFDTIKGSDYLADQDAVEVMCREAPGIVYEMEHW
                     GCPFSRFPDGSIAQRPFGGAGYPRTCYSTDLTGHVLLNTLYERAVMKGVRVYPEWYVT
                     ALATHDGVCHGVAAFDLMNGEIVPITAKATVFATGGYGRVYFYSTNALINTGSGIGIA
                     YKAGVPLKDMEFVQFHPTGLIGTNILMTEACRGEGGYLVNNRDERFMERYAPKAMELA
                     PRDIVSRSIQTEINEGRGFDHPLGTYIKLDLTHLGEKKILAKLPGIRKICIDFIGIDP
                     IREPIPIMPCQHYSMGGIDTNAKTATEVRGFFAAGECACVSVHG"
BASE COUNT          697 a         1024 c          887 g          706 t
ORIGIN      
        1 cccctttccc gacaaagaag ccgcaatacc tggggtcgac cctgacgatg gcttctctca
       61 ggccccgtga atagaccacc tccaccacct tatccggcgg cgcgggaaca agggaattga
      121 ctatgtattt ctccatgtcc gtgctgtacc ggacaatggt gatggtatcc gcggtgtacg
      181 ccttcacccc ctccaggtag ggaagcgacg cggagagcgg atccacgtct ccgaggccct
      241 cgacggaaac cttggcaaag gggccgttga tcacgctcgc cgcccttcgc acccgaaccc
      301 tgccgtcgcg tatgatggga gacagcgcct cctccgtgat ccggcggaag tactgcggtg
      361 acctctggct cagaaatatc ttcccctttc ccatcatgaa caccgcggcc accgtctcct
      421 ccccctgcct gtattccctg cccgcgaacc tcttcggaag gtaggccaag aggtccgtcc
      481 ccgtcacaat gacaaggtag acgtccctgt cggcaaggtc cttcacgata gcgcagcgca
      541 caaggcgacc tatcttgatc gtcgtcgtca tgatctcccc cctgagatat actcactgca
      601 caggtgtatt acttccggga gagaaaaaag gacccgaaaa aaagcaaaag cgggttatgg
      661 gtaataggtc atgggtgttg gggaaatgca tcaataccgg caaacattcc agggttcttc
      721 ccataccccg tgacccatta cccgcttttc gtaaccttga aactacgggg tcttccctat
      781 atactgttcg tcaaatatcc gcgcgaggtg attgagatgg aatgcacgat ggagaagaac
      841 aaggccgcct gcgcctgtac ttacgatccc tgcccccgga aaggaaaatg ctgcgaatgc
      901 gtcgcctacc accgggccag gaaacagctg cccggctgct tcttttcaaa ggaaggcgag
      961 gcctcctacg accggtcata caggcggttc gtgatggaaa atagttgaaa aaacagtttt
     1021 aagtgttagg ttttaagttt tacgtgaaag acctttcgct tgagaggatc tttgtacgtt
     1081 caacggaatg cgggaaagtg tcctaaggct tttaacttaa aacctaacac ataaaactta
     1141 acaccgtctt ttctattctt ccagttcctc gatcacttcc tgggccttta tgcggacctc
     1201 ttttatcttg tgggccatgg cggcgatgga gatcttccgc ctggcgtcct ccttctgacg
     1261 cggcggcaat gacgcgtaga gttcacggag cttgtcgagg gtggagttgc acacatctgc
     1321 gtccttcagg tccaggaaga gaatgagcgt gggcccgtcg tcgggcaggc catactttgc
     1381 catgtatctc tcggcctgct tcggaaaggc ctccgacccg taggcgttgt gcagcctggc
     1441 aaagagcttg tcgtgctcgg gcgttccctt gtcgcccttg aagagcctgt ccagggcatc
     1501 cttcactctt ccctgttgcc agcggtcctt tggagcttcc ttcttttttc cggcaccctt
     1561 ttcctgcctg ccgtaaaagg tactcctgtc cctgcggcgg tccagttccc gccaatcagg
     1621 tctttcgtcg tcgtagtctc ccatagctac ctcatcgagg gtttttttct ttattatacc
     1681 ccttccctat cgagattcca cccctttctc cgatagaacc ccggagccga aggaactcca
     1741 cctacctccc acgacaggcg caacgcaaac gaatacggaa cgttgaagcg gttcaagcgg
     1801 ttcaagcggt ctccaaatcc cttgacaaag ctcggcgcgc catgatgaga ttgtctgaaa
     1861 aggaagccaa ggagcggcca tgttcatatg gctttttcac cggataagcg gcgttgccct
     1921 tatcgttctt ttcggcatca agatactcac gagctatttt cttttcactg aggacaagaa
     1981 gcccgattgg gcgctgagcc tccaccggca gcccgttctc gacgtgctga tcctccttct
     2041 cttcaccttc cacagcatct acggcatccg cacaatagtc atggacctcg gctacaggaa
     2101 cgaaaagagg ctcttcgtgg cctccaacgt ggtcgcctcc gtcatctcgg cggtcctcct
     2161 gtatctttat ctcgtgatct catgacgggc ctctcccacg atgtgctgat agtcggcgga
     2221 ggcctcgcgg gattgcgggc ggccgtgggt ctctgcgaca aatatgacgt tgccctgctg
     2281 tccaaggtcc atcccatacg gtcccattcg atcgcggccc agggcggcat caacgcggca
     2341 ttggccaaca accccgacag cagggacgac acgtgggaaa aacacgcctt cgacaccatc
     2401 aagggcagcg attaccttgc cgaccaggac gccgtggagg tcatgtgccg tgaggcgccg
     2461 ggcatcgtct acgagatgga acactggggc tgccccttca gccgctttcc cgacggttcc
     2521 atagcccagc ggcccttcgg gggagcgggg tacccgagga cctgctactc aacggacctc
     2581 acgggccacg tactcctcaa cactctctac gaacgggccg tcatgaaggg cgtcagggtc
     2641 taccccgaat ggtacgtgac ggccctcgcg acccatgacg gagtctgtca cggcgtagcg
     2701 gccttcgacc tcatgaacgg cgagatcgtc cccatcaccg cgaaggccac ggtcttcgca
     2761 acgggcgggt acgggcgggt ctacttctat tccaccaacg cgcttatcaa cacgggaagc
     2821 ggcatcggca tcgcctacaa ggcgggcgtg cccctgaagg acatggagtt cgtgcagttc
     2881 caccccacgg ggctcatcgg caccaacatc ctcatgacgg aggcctgccg cggggaaggc
     2941 gggtacctcg tcaacaacag ggacgagcgc ttcatggaga gatacgcccc gaaggcgatg
     3001 gagctcgcac cgagggacat cgtgtcgcga agcatccaga cggagatcaa cgaaggcagg
     3061 ggcttcgatc accccctcgg cacgtacatc aagctcgacc tcacgcacct gggagagaag
     3121 aagatccttg ccaaactccc gggcatacga aagatctgca tcgatttcat cggtatcgat
     3181 cccatcaggg aacccatacc catcatgccc tgccagcact attccatggg gggcatcgat
     3241 acgaacgcga agaccgcgac cgaggtgcgc gggttcttcg ccgccggcga atgcgcctgc
     3301 gtgagcgtcc acgg
//
LOCUS       DAOQTR010000224         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_33357_length_3293_cov_2.835392, whole genome shotgun sequence.
ACCESSION   DAOQTR010000224 DAOQTR010000000
VERSION     DAOQTR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33357_length_3293_cov_2.835392"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..806
                     /locus_tag="PKN85_06310"
     CDS             288..806
                     /locus_tag="PKN85_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44055.1"
                     /translation="MDRERIERIIENKYSLLLFFLVILLAGSAFTAHNDMMHYTVGSL
                     FAMVVFIIMLKILRASRLLFAAYITLSFLALLLHYLAVFVVHSKSLGTVAIIVYILMI
                     GILIVFMTRRIFSERLVTGDTIKGGISVYIMMGTWWQMVSSCCGCAIPGPSFSPRARS
                     VRVTSCITASAP"
     gene            complement(1109..1348)
                     /locus_tag="PKN85_06315"
     CDS             complement(1109..1348)
                     /locus_tag="PKN85_06315"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNT44056.1"
                     /translation="MGGSVYVVNEIVGTSTTSWEDAAKTAIETAAKSVHDLRIGEVVT
                     QDVTLENGKVVSYRVRLNISFKYHPDKVWHEEVKR"
     gene            1475..1954
                     /locus_tag="PKN85_06320"
     CDS             1475..1954
                     /locus_tag="PKN85_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT44057.1"
                     /translation="MEIGRAIKERAEMLEDTSLSMDFSWEELVTLAGYMGTMTYRKGA
                     TIFEEGDREIFMCVVVKGGVAILKKDERMDPKRLTVIGLGKVIGEMSMIDGSPRSATA
                     TAAADTILVVLSKESMDRLFKEKPHVGLKLLKKIAHSMSQRLRQTTGVLVDYLERSG"
     gene            complement(2013..>3293)
                     /locus_tag="PKN85_06325"
     CDS             complement(2013..>3293)
                     /locus_tag="PKN85_06325"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNT44058.1"
                     /translation="ALTLAVDPLFQVLISAGDAVTFLEFRFQPGMALNLGHEEALLVA
                     HRPAVLGDGKVALDVAEKNFVRHNDLYRRRFFPDIPPRQEEKLPDDVYTEIAYAVQKA
                     TEDALIEMARHLYRISPSKNLCYAGGVGLNSVANKKILDNTPFERIFIQPGACDSGIA
                     LGCALHGAHIIHREPPGKYRLRNAYLGRAYSEEEILADLDQVKNIRFRKERDVALKAA
                     ELLAGGKILGWFEGGSEIGPRALGHRSIICDPRKPEMKDTLNARVKHREGFRPFAPSV
                     LLENASEYFDLDCESPYMLLIAEVKEDKRHLIPAITHVDGTARVQTVTREDNGRYYDL
                     ISEFGRITNVPVILNTSYNIAGEPIVETPGDALKCFMSTEMDYLIIEDYIIEATGPKE
                     LIHGPVIDENKLKAVDIRQNQWLGKLFSKKKKTL"
BASE COUNT          648 a          915 c          987 g          743 t
ORIGIN      
        1 ggacgaactg gaggtatatc tcggcaagca cagtgaagcg accttcagcc acgggctttg
       61 tccgagatgc agggaagaag tcgaaaaggg catgaagggc gaatagaaaa ggtcccgcat
      121 gtcttgaggc actcccacgt tctctcttcc atcttgtaaa ttgtcagcat ttgctgcaca
      181 ataaaatgtc agcccgccac aggcggtatt ctacgttggt gacagggaag gcggggagat
      241 gaatagatcg gcccgtcggc agggaagatc ctctcaaggg ggcacggttg gacagagaac
      301 gcatcgaacg cattatcgag aacaagtatt ccctcctcct cttctttctc gtcattctgc
      361 tggcgggttc agcttttacc gctcacaatg acatgatgca ttacaccgtt ggttcgctct
      421 tcgcgatggt cgttttcata atcatgctga agatcctgag ggcctcccgg ctcctctttg
      481 ccgcctatat aaccctgtct tttctggcgc tgctcctgca ctacctcgcc gtgttcgtcg
      541 tgcacagcaa gtccctggga acggttgcca ttatcgtcta catactgatg atcgggatac
      601 tcatcgtgtt catgacgcgt cggatattct ccgagaggct ggtaacgggc gataccatca
      661 agggcgggat ctccgtgtat ataatgatgg gtacgtggtg gcagatggtc tccagctgct
      721 gtggatgtgc gatccccggt ccttcctttt ccccgagggc acggtcggtc agggtgactt
      781 cctgtattac agcatcagca ccataacatg cctgggattt ggtgacatac taccccggtc
      841 ctatccggca aagctcttct ccgtgatcga ggcccttgtg ggtcagcttt acgtggcggt
      901 ctttgttgcc cgtctcgtag gactgcacgt ggcggtacac gcacggaagc acaacgacgg
      961 acgctagccg gcattaccgt ctttctcccg atcaggcaag gcttctgccg cggtgaagcc
     1021 cgcggggggt gccgccggat atctcacctt cgaggaacgg ggccgggcgg cgggtacgtg
     1081 gtctgaggtt gcagggaatg tctcttgctc agcgcttgac ctcctcgtgc cagaccttgt
     1141 ccgggtgata cttgaaggag atgttgagcc tgacgcggta gctcaccacc ttaccgttct
     1201 ccagggtcac atcctgggtc acgacctctc cgatacggag gtcatgaacg cttttggcgg
     1261 cggtctctat ggccgtcttt gccgcatcct cccatgatgt cgtgctggtg cccacgatct
     1321 cattgacgac atacacgctt cctcccatga caacctcctt gctgtccgcg agtgttataa
     1381 ggtgttaata gcacaaatga aaggcgtgtc aagaagggcg agccttcagt ccggcccgga
     1441 aatggcgata atactgcatt gaggagagag aagaatggag atcggacgtg ccataaagga
     1501 acgggcggag atgctcgagg acacatcgct gagcatggat ttttcgtggg aggaactggt
     1561 cactctcgcg ggttacatgg gcaccatgac ctacaggaag ggagcgacga tcttcgagga
     1621 gggcgacagg gagatcttca tgtgcgttgt tgtgaagggc ggcgtcgcta tcctgaagaa
     1681 ggacgagcgc atggacccga agcgcctgac cgttataggt ctgggaaagg tcattggcga
     1741 gatgtccatg atagatggga gccctcgctc cgcaacggcg acggccgccg ccgataccat
     1801 cctggttgtc ctctcgaagg aaagcatgga ccgtcttttc aaggaaaaac cccacgtcgg
     1861 cctcaaactg ctcaagaaga tcgcccattc catgagccag agactgcgtc agacgacggg
     1921 cgtgctcgtg gattatcttg aaagaagcgg ctgagtcatc ccgtcagccg aggcggcacc
     1981 ggggacggac cgggatggac ctctgacacc ggtcacagcg tctttttctt cttcgagaag
     2041 agcttcccca accactgatt ttgccggata tcgaccgcct tcagcttgtt ctcgtcgata
     2101 acggggccgt ggatgagttc cttgggccct gtcgcctcga tgatgtagtc ctctatgatg
     2161 aggtagtcca tttccgtcga catgaagcat ttcagggcat cccccggcgt ttcgacgatc
     2221 ggttccccgg cgatgttgta cgacgtgttg aggatgacgg ggacattcgt gatcctgccg
     2281 aactcactga taaggtcgta gtacctccca ttgtcctctc gtgtgacggt ctggaccctg
     2341 gcagtaccgt ccacgtgagt tatggcgggt atgaggtgcc gcttgtcctc tttcacctcg
     2401 gcgatgagga gcatataggg actctcgcaa tcgaggtcga aatactcgga ggcgttctcg
     2461 agaaggaccg acggcgcgaa aggccggaaa ccctcccggt gctttacccg ggcgttgagc
     2521 gtatccttca tctccggctt tctcgggtcg cagatgatac tcctgtggcc cagggcgcgg
     2581 gggcctatct cgctgccccc ctcgaaccag ccgaggatct tgcccccggc caaaagctcc
     2641 gcggccttga gcgccacatc ccgctccttc ctgaatctga tgttcttcac ctggtcaagg
     2701 tcggcgagga tttcctcctc gctgtatgcc cggcccaggt aggcattgcg gaggcgatac
     2761 ttgccgggag gctcccggtg gatgatatgg gcgccgtgaa gggcgcatcc cagggcgatc
     2821 ccggaatcac aggctccggg ctggatgaat atcctttcga aaggcgtgtt gtcgagtatc
     2881 ttcttgttgg cgacgctgtt gaggccgaca ccgccggcgt agcacaggtt cttcgagggg
     2941 cttatccggt agaggtggcg ggccatttcg atcagggcgt cttccgtcgc cttctggacg
     3001 gcataggcga tctcggtgta gacgtcgtcg gggagctttt cctcctgccg cgggggtatg
     3061 tccgggaaga agcgcctgcg gtacagatcg ttgtggcgga cgaaattctt ctcggcgaca
     3121 tcgagcgcga ccttgccatc gccaagaacg gcgggccgat gcgcgaccag cagggcttcc
     3181 tcgtgcccga gattgagcgc catgcctggc tggaagcgaa actcaagaaa cgtaacggcg
     3241 tcacccgcag atatcagtac ctgaaaaagt gggtccaccg cgagagtcag cgc
//
LOCUS       DAOQTR010000225         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_33372_length_3292_cov_3.191535, whole genome shotgun sequence.
ACCESSION   DAOQTR010000225 DAOQTR010000000
VERSION     DAOQTR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33372_length_3292_cov_3.191535"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1066
                     /locus_tag="PKN85_06330"
     CDS             149..1066
                     /locus_tag="PKN85_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT44059.1"
                     /translation="MRHVFGPVPSRRLGYSLGVDIIPPKHCTYDCIYCQIGKTTNREI
                     GRKSFYDAGLIADEVARKVGSTRVDVITFSGSGEPTLNSDLGTMIRKVKERVDTPVVV
                     ITNGSLLYEKDVRSDLGEADMVLPSLDAVTEEVFQRINRPHPLLDITRIIEGLREFRA
                     EYTKPIWLEIMLIKNVNDDPDELKKMAQIVSTLNVDRIQLNTVTRPPSEESARRLEEE
                     DLQATCRLFGPRCEVICSFERTGEIPAQADWTRIILETLRRRPLTLDDVVRTTSLSRL
                     QAETKLRAMEDEGLVRSYVLGDDLFYVVA"
     gene            1226..1810
                     /locus_tag="PKN85_06335"
     CDS             1226..1810
                     /locus_tag="PKN85_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44060.1"
                     /translation="MKIRRAVVSVSSKEGLSDLALFLNKLGVEILSTGGTRKYLEGIG
                     LGPIEVSTYTGFPEIMDGRVKTLHPKVHGGILNIRDNEEHQQAMRTHGISHIDMIVVN
                     LYPFIEVVSKGCTFEDAIENIDIGGPSMIRAAAKNFKYVTVVVDPEDYSKVMENMAQN
                     DGATTEELRFYLAKKVFALTSAYDKAITDYLSKI"
     gene            1829..3115
                     /gene="purD"
                     /locus_tag="PKN85_06340"
     CDS             1829..3115
                     /gene="purD"
                     /locus_tag="PKN85_06340"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNT44061.1"
                     /translation="MNVLVIGSGGREHALCWKLSTSTGVDKLYCVPGNGGISDIAECH
                     DINVGDTAGLLEFTKRMKVDLVVVGPENPLAGGIVDAFEAKGIPIFGPVARGAAIEAS
                     KIFSKELMRDSSVPTAPFQVFDDHKNALGYIRAKEPPFVIKADGLCAGKGAYVIKDTA
                     EAESVLTELMVNGIYGEAGKKVVIEEFLPGVEASYLAFADGNTILPMPASQDHKPLLD
                     NDLGPNTGGMGAYTPIPFLNDGLEREIREGIMLKTIEAMREKGISYKGVLYGGLMLSG
                     GRPYVIEFNARFGDPETQPIIFKMESDLLPVLVACVEGRLRDVGEIRWKEGVSVCVVL
                     ASKGYPEKPEKGKVIRGLDRLAGKEDIMVFHAGTKKAGSEYVTSGGRVLGVTALGSTY
                     EDAVNKAYEAVNLIDFEGMHYRRDIGKKALTVGRTL"
BASE COUNT          775 a          838 c         1002 g          677 t
ORIGIN      
        1 acgacctctg gtatgtgaga ccagcgctct aaccatctga gctaatcgcc cgtgcgtttc
       61 tttttattac attcttgtga aaaagtcaac ccaagacagg aaggatttct gttcaaatct
      121 tcgatgggat cgtataatat tctgcattat gcgacatgtt ttcggtccgg tcccttcgag
      181 gagactgggg tactccctcg gcgtcgatat catcccaccc aagcactgca cctacgactg
      241 tatctactgc cagatcggca agaccacgaa cagggagata gggcgaaaga gcttctacga
      301 tgccgggttg atagcggacg aggtggcacg gaaggttggc tcaacgcgcg tggatgtgat
      361 cacattttcg ggttccggcg aacccaccct gaactcggat ctgggcacca tgatcaggaa
      421 ggtcaaagag agagtcgata cgcccgtggt ggtcatcacc aacggatctt tgctgtacga
      481 gaaggatgtg aggtcggacc ttggggaagc cgacatggtc ctgccctcac tcgacgccgt
      541 taccgaagag gtcttccaaa ggatcaacag gccccatccg ctccttgata tcacgaggat
      601 catagagggt ctgagggaat tcagggcgga gtatactaag ccgatatggc tcgagatcat
      661 gcttatcaaa aatgtcaacg atgaccccga tgagttgaaa aagatggcgc aaatcgtctc
      721 gacactgaat gttgacagga tccagctcaa caccgtcacg cggccgccga gcgaagagag
      781 cgcgcgcagg cttgaagagg aagaccttca ggcgacatgc cggctcttcg gcccacggtg
      841 tgaggtgata tgctcctttg aaagaacagg ggaaataccc gctcaggctg actggacgcg
      901 gatcatcctg gaaactttga ggagacgtcc cctgactctc gacgatgtgg tcaggacgac
      961 gagtctttcc cgtttgcagg cagaaacgaa gctgagagcg atggaggacg agggcctcgt
     1021 caggagctac gtcctgggcg atgacctttt ctatgttgtc gcatagccgc tttgtcctgc
     1081 tccccatccc tttttccgcc cccaaaatca tgttgacaaa ggagtgagcc agcatttact
     1141 attacagctt agagagcggc agacacgcag ttttcccttg tctttacccg tatattcttg
     1201 attatatcgc gcgaaggagg ttgttatgaa gatcaggcgt gccgtggtca gcgtttccag
     1261 caaggagggt ttgtctgacc tggcgttatt cttaaacaag ctcggcgtcg agatactgtc
     1321 gacggggggc accagaaagt atctggaagg gatcggactt ggtcccatcg aggtcagtac
     1381 ctacacgggt tttcccgaga tcatggacgg ccgggtgaag acgctgcatc ccaaggtcca
     1441 cggcgggatc ctgaacatcc gtgacaacga ggagcaccag caggcaatga gaacgcacgg
     1501 gatcagtcat atcgacatga tcgtcgtcaa tctctatcct tttatcgagg tggtgagcaa
     1561 ggggtgcacc ttcgaggacg ccatcgagaa catagacatc gggggcccct cgatgatccg
     1621 tgccgccgcc aagaacttca agtatgtcac cgtcgtcgtc gatcccgagg actattcgaa
     1681 ggttatggag aacatggcac agaatgacgg cgccacgacg gaggaattga ggttctacct
     1741 ggcgaagaag gtcttcgctc tcacaagcgc ctatgataag gccatcaccg attacttgtc
     1801 gaaaatctaa ctgaacccgg gggttggcgt gaacgttctt gtaataggat caggagggag
     1861 agagcacgcg ctgtgctgga agctctccac gtcgacgggc gtcgacaagc tgtactgcgt
     1921 tcccggtaac ggcggcatct cggacatagc cgaatgccat gacattaatg tgggagatac
     1981 ggccgggctt ctcgaattca cgaagaggat gaaggtcgac ctcgttgtcg tcggtccgga
     2041 gaaccccctt gcggggggga tcgtcgacgc cttcgaggcg aagggtatcc ccatattcgg
     2101 tcctgttgcc cggggcgccg ccatcgaagc gagcaagatc ttctcgaagg agctcatgag
     2161 ggacagttcg gtgcccacgg cgcccttcca ggtcttcgac gaccacaaga acgccctggg
     2221 atacatacgg gcgaaggagc ccccctttgt tatcaaggcg gacggcctct gtgcggggaa
     2281 gggcgcttac gtcatcaagg atacggcgga ggcggaatcg gtcctcacag agctcatggt
     2341 caacggtatc tatggcgagg ccggcaagaa ggtggtcatc gaggaattcc tgcccggtgt
     2401 ggaggcgtcc tatctggcct ttgcagacgg caacacgatc cttcccatgc ctgcctcgca
     2461 ggaccacaag cctcttctcg acaacgacct cgggccgaat acggggggga tgggcgcgta
     2521 cacgccgata cccttcctca acgacggtct ggagcgtgag atacgtgagg ggatcatgct
     2581 caaaacgata gaggccatgc gcgagaaagg catctcctac aagggggttc tctacggggg
     2641 attgatgctc tcgggcggaa gaccctacgt tattgaattc aatgcgcgtt ttggcgatcc
     2701 ggagacacag ccgataatct tcaagatgga gagcgatctg ctccccgtcc tcgtcgcctg
     2761 tgtggagggc agactcaggg atgtgggaga gatacggtgg aaagaaggag tgagcgtgtg
     2821 cgtcgtcctc gcctcaaagg gatacccgga gaagccggag aaaggcaagg tgatccgggg
     2881 cctcgacagg ctggcgggga aggaggacat catggtattc cacgccggca cgaagaaggc
     2941 gggttcggaa tacgtgacct ccggtggccg ggtcctcggt gtgacggcac tggggtcaac
     3001 gtatgaagac gctgtcaata aggcctacga ggccgtgaat ctcattgatt ttgaggggat
     3061 gcactatcgc agggatatcg ggaaaaaagc cctgacggtg gggcggacgc tataaagaca
     3121 agaaaggcag gaggtttcaa tgtcgaagga attcaatatc ggtgtgatac ccggcgacgg
     3181 gacaggccct gaggttgtgg ccgagggtgt caaggttctc acggccgcat cgaagaagtt
     3241 cggtttttcg ctgaaactca catattacga cataggcggg gaacgctaca ag
//
LOCUS       DAOQTR010000226         3284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_33493_length_3284_cov_4.116135, whole genome shotgun sequence.
ACCESSION   DAOQTR010000226 DAOQTR010000000
VERSION     DAOQTR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33493_length_3284_cov_4.116135"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PKN85_06345"
     CDS             <1..1162
                     /locus_tag="PKN85_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HNT44062.1"
                     /translation="KPFAPALISWSVAMLPKHLLEGKDITTSPLKRKPVGTGPFVFTE
                     WVPGDRVILTANPDYFEGRPYIGRYMMKIIPDTATMFLELKNSSIDLMGLTPLQYVKQ
                     TDYPAFEREFNRFKFLASVYTYIGYNLRSGLFKDKRVRQALAHAINKQEIIDGILLGQ
                     GVEAMGPFKPDMWAYNGNVKRYDYSPEKALALLKEAGYERDADGVLKREGRPFEFTIL
                     VNQGNVVRIQSAELIQRRLSAIGITMKIRVLEWATLVNDFIDKRSYEAVLLGWTIPND
                     PDPFDVWHSSKQGPKELNFIGFENKEVDDLLVRARHTLDRGERKKYLDRLQEVLAEEQ
                     PYTFLFVPYSTVALHKRFKAVEPAPAGIMYNFIKWYVPENQMRYRSNAAVSP"
     gene            1143..2117
                     /locus_tag="PKN85_06350"
     CDS             1143..2117
                     /locus_tag="PKN85_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT44063.1"
                     /translation="MLRYLLKRLFFMIPMVFGITLISFAVIHLVPGEPGALEAEMNPK
                     ITKEARERIRSFYGLDKPLYVQYWSWLTRIVRLDFGTSFATDRRPVIDKIKERMPITI
                     LINLLSMGLIFIFGIPIGIYCARYKDTFLDRFLTSFVFAGYAAPTFWVALLSMIFFGV
                     YLEVLPVSGIKSYDFGEMNIFEKILDVTRHLVLPVCISAVGSLAGISRYMKSSLLEVL
                     RQEYITTAYAKGLPESMVIRKHALRNALLPVITILGLSIPGLIGGSVIFESIFSIPGM
                     GQLFYFSVMARDYPTIMGILVIGAFLTLVGNLVADVSYAVADPRIRIG"
     gene            2259..3158
                     /locus_tag="PKN85_06355"
     CDS             2259..3158
                     /locus_tag="PKN85_06355"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNT44064.1"
                     /translation="MKKHLKNLAPVARGLSTTAKKVFKKYQADHANIMVSSIAFYILL
                     TFIPFTLFSFFILGYVIDVGHTGVQMEKYIARAIPAPYNAVIVKKVLREINVISLSKR
                     LSGPLGLLFLLLFTSRLFAVLRPSFQLIFGKTKKGFIKGKQEEFVLTFAFALIQTIIF
                     FSYVFSVMMQVKVVKAFGGAVTRASVVQFFSVLDLFLVAGMFWFLYYVLTPVRDRRRL
                     LFVSALGAAMWHAGKYFFQYFVLYIGRFTTFFGTYGVFIAFLFWVYYSVFVFIVCAEL
                     LAVSSEPAVSGPVPNYARFRWKR"
BASE COUNT          673 a          964 c          863 g          784 t
ORIGIN      
        1 caaacccttt gccccggccc tgatctcctg gtccgttgcg atgttgccga agcaccttct
       61 cgagggaaag gatatcacca cctcacctct gaagcgcaaa ccggtgggta cgggaccctt
      121 cgtattcacc gaatgggttc ccggcgaccg cgtcatcctc accgcgaacc ccgactattt
      181 tgagggaaga ccctacatcg gacggtacat gatgaagatc atccccgata cggccaccat
      241 gttcctcgaa ctcaagaaca gttccatcga tctcatgggt ctcacgcccc ttcagtatgt
      301 gaagcaaacc gattacccgg cgttcgagcg ggaattcaac aggttcaagt tccttgcctc
      361 tgtctacaca tacataggct ataacctgcg cagcggcctg ttcaaggaca aaagggttcg
      421 tcaggccctg gctcacgcga tcaacaagca ggagatcatc gacgggatac tcctcggtca
      481 gggggtagag gcgatgggtc ccttcaaacc cgacatgtgg gcatataacg gaaatgtgaa
      541 gcgatacgat tacagcccgg agaaggccct tgcccttctc aaggaggctg gatacgagcg
      601 cgatgcagac ggggtcctca agagggaagg gcggccattc gagtttacca ttcttgtcaa
      661 ccaggggaac gtagtgcgga tccagagcgc ggagttgatc cagcgcaggc tctcagccat
      721 cgggatcacg atgaagatcc gcgttctcga atgggcgacg ctcgtcaacg acttcatcga
      781 caagaggagc tacgaggcgg tccttctggg atggacgata cccaacgatc ccgacccctt
      841 cgatgtctgg cactcctcca agcagggacc gaaggaactg aacttcatcg gcttcgagaa
      901 caaggaagtc gacgatctgc ttgtcagggc ccgtcacacc cttgaccggg gggagaggaa
      961 gaaatacctt gaccgtctcc aggaggtcct tgccgaagaa cagccctaca cgttcctttt
     1021 tgttccgtac agcacggtgg ccctccacaa gcgcttcaag gcggtggagc cggcgcccgc
     1081 gggcatcatg tacaatttta tcaagtggta cgtgccggaa aaccagatga ggtacagatc
     1141 gaatgctgcg gtatctcctt aaacgtcttt ttttcatgat ccccatggtc ttcgggatca
     1201 ccctcatctc ctttgccgtg atccatctcg tgcccgggga gcccggggcg ctcgaggccg
     1261 agatgaaccc gaagatcacc aaggaggctc gggagcgcat ccgctccttc tacggcctcg
     1321 ataaaccgct gtatgtccag tactggtcat ggctcacgag gatagtgcgc ctcgacttcg
     1381 gcacatcctt cgccacggac aggcggcccg tcatcgacaa gatcaaggag aggatgccca
     1441 tcaccatact catcaatctc ctctccatgg ggcttatctt cattttcggc atacccatag
     1501 gcatctactg cgcccgctac aaggatacgt tcctggacag gtttctgacc tcgttcgtgt
     1561 ttgccggata tgccgctccg accttctggg tggcgctgct ttccatgatc ttcttcgggg
     1621 tctatctgga ggtccttccc gtgtcgggca tcaagtccta cgacttcggg gagatgaata
     1681 tcttcgagaa gatcctcgac gtgacgagac atctcgtgct gcccgtctgc atctccgcgg
     1741 tgggcagtct cgcgggcatc tcacggtaca tgaagagcag tctcctcgag gtcctgagac
     1801 aggaatacat caccacggca tacgccaagg ggcttcccga gagcatggtc ataaggaagc
     1861 acgccctgcg caatgccctc ttgcctgtca tcaccatcct cgggctttcc atacccggtc
     1921 tcataggcgg cagcgtcatc ttcgaaagca tcttttccat ccccggcatg ggacagcttt
     1981 tctacttcag cgtcatggcc agggattacc ccacgatcat ggggatcctt gtcatcggcg
     2041 ccttcctgac gctcgtcggc aacctggtcg cggacgtctc ctacgcggtg gcggatccaa
     2101 ggataaggat aggataaaga cggttccaag gttcaaaagt tcaagggttc aaggatgaag
     2161 agcctgattt tctcgatagg tcctatcggt cccatatgtc ccataggtct tataattcct
     2221 ataggtccta ttgctgcccc ctgttctccc ggtgcgtcat gaagaagcat cttaagaacc
     2281 tggcgcccgt ggcgagaggg ctttccacca cggcgaagaa ggtctttaag aaataccagg
     2341 cggaccatgc caacatcatg gtctcttcca tagcattcta catcctgctg accttcatac
     2401 ctttcacgct cttctccttc ttcatcctcg gatacgtcat cgatgtgggc cataccggcg
     2461 ttcagatgga gaaatacatc gcacgggcca taccggcacc ctacaacgcg gtcatcgtca
     2521 agaaggttct cagggagata aacgtgatct cgttgtccaa gaggctttcg ggtccactgg
     2581 gcctcctctt tctccttctt ttcacatcga ggctctttgc cgtcctgcgc ccctcttttc
     2641 agctcatatt cggcaagacg aagaaagggt tcataaaggg gaagcaggag gagttcgtcc
     2701 ttacctttgc ctttgccctt atccagacca tcatcttttt cagctacgtc ttttccgtga
     2761 tgatgcaggt gaaggtggtg aaggccttcg ggggggccgt cacgcgcgct tcggtggtgc
     2821 agttcttttc cgtcctcgat ctctttcttg tcgccgggat gttctggttt ctctactatg
     2881 tcctcacgcc cgtgcgggac aggaggaggc ttctcttcgt ctccgccctg ggggccgcga
     2941 tgtggcacgc gggcaagtac tttttccagt acttcgttct ctacataggg cgattcacga
     3001 ccttcttcgg cacctacggc gtcttcatcg cctttctctt ctgggtctat tactccgttt
     3061 ttgttttcat cgtttgcgcg gagctgctgg cggtctcctc ggaaccggcc gtcagtgggc
     3121 ctgtgcccaa ctatgcccga ttccgatgga aacggtgaga gggaccttaa gctcgatcac
     3181 cttttccatc tcctgcctga cgagctgttt catcgtttct gtctcgtcga gggtgacctc
     3241 gaagagcagt tcgtcatgta tctgcaggat aagccgcgaa gaga
//
LOCUS       DAOQTR010000227         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_33789_length_3264_cov_3.414148, whole genome shotgun sequence.
ACCESSION   DAOQTR010000227 DAOQTR010000000
VERSION     DAOQTR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33789_length_3264_cov_3.414148"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKN85_06360"
     CDS             complement(<1..197)
                     /locus_tag="PKN85_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44065.1"
                     /translation="MSMTLEDAREMIEDVFDFDHVGDAMTALRRDLGSSAAKEELVDR
                     LTKYALEEGDDVSGETRAVLL"
     gene            complement(238..558)
                     /locus_tag="PKN85_06365"
     CDS             complement(238..558)
                     /locus_tag="PKN85_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNT44066.1"
                     /translation="MNEERSKKDGGGEKCYAFPNRPTPGMPVVIDETRCTGCNKCVTI
                     CTTDVLVANPEKGKPPIVMYPEECWYAACCVGECPEECLTMRHPLMMRVHFKNKETGE
                     IKRT"
     gene            complement(579..2567)
                     /locus_tag="PKN85_06370"
     CDS             complement(579..2567)
                     /locus_tag="PKN85_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNT44067.1"
                     /translation="MNWEDAVTQTGQVIEWPYPVRYDRQNDIDVDVVVLGGGPGGCMA
                     AISAAKKGLKVALLDKAYPKRSGGGSGFDHWLNTPNPASKVTAEDCVEWERVTFKGYS
                     NSLSRYVAAREAYDTLLEIEKMGAKVRDIDDTFKGAPFRDEKTKFVYCYDYENRFQFR
                     VWGATFKPAIFNECLQLGVQVFDRVVATSLLTEGGKQGARVVGATAINMRTGEFYVFR
                     AKATINAMSCHQGSWRFSTESIGLDMFKPNITGDGPAIMWRAGVELAQMERSRAAMPP
                     GYEYPSYGTGNWWNTWHPATIVDANGKEVPYVDSDGNVVPVERRCVPRPGQKFIAERS
                     RDPLYAKPTMPADLADRIRRGEFVLPLYADLAGMPWYERDAIWGLMVGNEGRTKIPVL
                     VNYETAGFNSHRDMLQNYFTLSGEPYPSMDSTMALGPYRMEAFGNGGEVVVDWDLRTN
                     LEGLYAVGDAILAGNYWYHACATGRYAGRKAAEYAIQLEGPPSIDAAQVERERERVYA
                     PTKRKPGKDWVDWKELRFAGARAMQNYCGAVRNEGMMKMGLKWIEDIKNDHYPEAHAS
                     NPHILLRVLETYNMLTVDEIIFQACMARKASSEVLGHFRQDYPEMDPPEWHKWITIKQ
                     VDGATRTGDLALDFQGDLAGNYEAHNPNYRGYLKKKKA"
     gene            complement(2795..>3264)
                     /locus_tag="PKN85_06375"
     CDS             complement(2795..>3264)
                     /locus_tag="PKN85_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNT44068.1"
                     /translation="GIVGKRAQLCLHAASDMLEGGELTLRYFYEARRAIECAIIRLAV
                     DKAKAKDLKGLREINARLLDEIDERDRLRANNADFHMTIAGIAGNPLLTMMLQSILEL
                     LDEVYPRSMQSEEYIKNTYARHDGIIKAMEKKDYARCDELMARDTEHTTKLVT"
BASE COUNT          564 a         1054 c          871 g          775 t
ORIGIN      
        1 tcaaggagaa ctgcccgcgt ttccccggaa acatcgtccc cttcttccag cgcgtatttc
       61 gtcaaccggt ccacaagctc ttccttcgct gcagacgatc caagatcacg cctgagcgcc
      121 gtcatcgcgt ccccgacatg gtcgaagtca aaaacgtcct ctatcatctc cctggcatct
      181 tccagcgtca tgctcatact accctccgtg tggcgggaca ttggccgtgc gccccggtca
      241 cgtccgcttg atctccccgg tttccttgtt cttgaaatgc accctcatca tgaggggatg
      301 ccgcatggtg agacattcct cggggcactc accgacgcag caggccgcgt accagcactc
      361 ctcggggtac atgacgatcg gcggcttgcc cttctcgggg ttggccacga gcacgtcggt
      421 cgtgcatatg gtaacgcact tgttgcaccc cgtgcatcgt gtctcgtcga tcaccacagg
      481 catgccggga gtcggtctgt tgggaaaggc gtagcacttc tcgccgccgc catctttctt
      541 cgatctctct tcgttcattc tcctcgcctc ctgcctcgtc aagccttctt ctttttcagg
      601 tatcccctgt agttcgggtt atgggcttca tagttcccgg cgagatctcc ctgaaaatcg
      661 agggcaaggt cgccggtcct cgtcgcaccg tcgacctgct tgatcgtgat ccatttgtgc
      721 cactcgggcg ggtccatctc gggatagtcc tggcggaagt gcccgaggac ctcgctgctc
      781 gccttcctgg ccatgcaggc ctggaatatg atctcgtcga cggtcagcat attgtatgtt
      841 tccagaacac gcagaagtat gtgcgggttg cttgcgtggg cctccgggta gtgatcgttc
      901 ttgatgtcct ctatccattt gaggcccatc ttcatcatcc cttcgttcct gacggccccg
      961 caataattct gcatggcccg ggcaccggcg aaacgcagct ctttccagtc gacccagtct
     1021 ttgccgggct tcctcttcgt gggagcgtag accctctccc gttcgcgctc aacctgggcc
     1081 gcatctatgg agggtggacc ctcaagctga atcgcgtatt ccgccgcctt cctgcccgcg
     1141 taccttcccg tggcgcaggc gtggtaccag tagttccccg ccagtattgc gtcgccgacc
     1201 gcgtagaggc cctcgaggtt cgtcctcagg tcccaatcga ccaccacctc tccaccgttg
     1261 ccgaaggcct ccatgcgata cggtccgagg gccatggtgc tgtccatgct gggatagggc
     1321 tcgccgctca gggtaaagta gttctgaagc atgtcacgat gagagttgaa ccccgccgtc
     1381 tcgtagttga caaggactgg gatcttcgtc cgtccctcgt tccccaccat gaggccccat
     1441 atcgcatccc gttcatacca gggcattccc gcaaggtcgg cgtacagggg aaggacaaat
     1501 tctcctctcc tgatgcggtc cgcaaggtcc gcgggcatcg ttggtttggc atagagcggg
     1561 tcccgggacc gctcggctat gaacttctgt cccggcctcg gaacacagcg ccgctccacg
     1621 ggcacgacgt tgccgtcgga atccacgtag gggacctcct tgccgttggc atccacgatg
     1681 gttgccggat gccacgtgtt ccaccagttg cccgtgccgt aggaggggta ttcgtatccc
     1741 ggcggcatgg cggccctgct cctttccatc tgcgcgagct ccacacccgc cctccacatg
     1801 atcgcgggcc cgtcgcccgt tatattgggc ttgaacatgt cgaggccgat ggactcggtc
     1861 gagaaacgcc agctcccctg gtggcaggac atggcgttta tcgtggcctt cgccctgaag
     1921 acatagaatt cgccggtccg catgttgatg gccgtggcac cgacaacacg cgcgccctgt
     1981 ttccctccct ccgtcaggag gctcgtggcc acgaccctgt cgaatacctg cacgcccagt
     2041 tgcagacatt cgttgaagat ggccggcttg aaggtggcgc cccagacacg aaactggaac
     2101 ctgttctcgt agtcgtagca gtagacgaat ttcgtcttct catccctgaa aggagccccc
     2161 ttgaacgtgt cgtcgatgtc ccgtaccttc gcgcccatct tttcgatctc gagaagcgtg
     2221 tcgtatgcct ccctcgccgc aacgtaccgt gagagggaat tggagtaccc tttgaacgtg
     2281 accctctccc attccacgca atcctcggct gttaccttcg atgccgggtt cggcgtgttg
     2341 agccagtggt cgaatccgga cccgccgccg gaccttttcg gatatgcctt gtccagcagg
     2401 gcaaccttca gccccttctt cgcggcgctg atggccgcca tgcacccgcc cgggccgccg
     2461 ccgagcacaa cgacgtccac gtcgatatcg ttctgtctgt cgtacctgac aggatagggc
     2521 cattcgataa cctgacccgt ttgcgttacc gcatcttccc agttcatatt acctccttca
     2581 agaacttcgt ggacctgtcg tgatcatcgt ggtgcgcgtt gagataaaat gccggaacga
     2641 cctgtctccc cgcaaaccga ctgtctgtct cacgttagta tggactaacc aacagaccat
     2701 gtgtaattat cgaccaaagc cggactgctt gtcaaggaat atttgtggga aagagagggg
     2761 agttcttccg gggtcctctc acggcccggt ccgtctacgt cacaagcttc gtcgtgtgtt
     2821 ccgtgtctct cgccatgagt tcgtcgcagc gggcatagtc cttcttttcc atcgctttga
     2881 tgatcccgtc atgacgtgcg taggtgttct tgatatattc ctcggattgc atcgatcttg
     2941 gatagacctc gtccagaagc tcgaggatcg actgaagcat catggtcaga agagggttgc
     3001 cggctatccc agctatggtc atatgaaagt cggcgttatt ggcacgaagc ctgtccctct
     3061 catcgatctc gtcaaggagc cgggcgttga tctccctgag acccttcaaa tcctttgcct
     3121 tcgccttgtc gacggccagc ctgatgatcg cgcattcgat ggcccttcgc gcctcgtaaa
     3181 aataccgcag cgtcaactcg ccgccttcaa gcatgtccga ggccgcgtgc agacacagct
     3241 gagcccgttt gccgacgatc ccct
//
LOCUS       DAOQTR010000228         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_33832_length_3262_cov_2.975055, whole genome shotgun sequence.
ACCESSION   DAOQTR010000228 DAOQTR010000000
VERSION     DAOQTR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33832_length_3262_cov_2.975055"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="PKN85_06380"
     CDS             complement(<1..1415)
                     /locus_tag="PKN85_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT44069.1"
                     /translation="MEVSRAHILVCAGAACVSSGCRQIRDAVVNKIVEQGLQNEIKVI
                     ETGCIGSCDLGPLALVYPEGVLYQKLTPADAEEIVTEHLLKGRIVERLLYKEAVSAKT
                     IPSMKEIDFFKNQIKIVLRNCGVINPMNIEEYIARDGYLALGKALTSMTREEVIDEVL
                     KSGLRGRGGGGFPTGLKWKFAFQSPGDVKYVVCNADEGDPGAFMDRSVLEGDPHSVIE
                     AMAIAAYAIGAQQGYVYVRAEYPLAIERLNWAIGQAREYKFLGKNILETDFNFDIEIR
                     IGAGAFVCGEETALMISIEGKRGEPRPRPPFPVVKGLFNAPTLLNNVETYANIPAIIN
                     NGAAWYAQYGTEKSKGTKVFALAGAVNNTGLVEVPMGTPLGDIVFNIGGGIKGGKKYK
                     AAQLGGPSGGCIPIDHLNVPVDYESVAELGAIVGSGGLIVADEDTCMVDFARFFLDFI
                     QHESCGKCPPCRIGTKRMLEI"
     gene            complement(1431..1925)
                     /locus_tag="PKN85_06385"
     CDS             complement(1431..1925)
                     /locus_tag="PKN85_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT44070.1"
                     /translation="MSEKQCHCAEVQEEELYPRLEEVIMEHRGKGEDLIMVLHKAQNL
                     FGYLPRKAQEMVAEGLNVSLNEVYGVITFYNFFSTVPQGRNSVKVCLGTACYVRGSQE
                     VLDKAQRELGIKVGGTTEDRRYSLDVVRCIGACGLAPAMLVNEDVYGRIKPPHLMDVL
                     EKYE"
     gene            complement(1964..>3262)
                     /locus_tag="PKN85_06390"
     CDS             complement(1964..>3262)
                     /locus_tag="PKN85_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNT44071.1"
                     /translation="EVCSRVQTVNALAVNHRGFDTVITTAFNAGLGNSVCINCGQCVV
                     HCPVGALYEKSAVDEVWDAIHDPDKFVVVQEAPAVRVQLGEEFDMPTGSLVSGKMHAA
                     LRRLGFDAVLETNFTADLTILEEGTELIGRLTSGKNLPLITSCSPGWIKFMETFFHDM
                     IPHMSTAKSPQQMFGALAKTYYAEAKGIDPAKIVSVSVMPCTAKKFECLRPEMNASGH
                     RDVDYVLTTRELGRMMREAGLDIREMPDEPADDLLGDYTGAATIFGATGGVMEAAVRS
                     AYRLVTGRELEDVEIKPVRGLKGIKEAEIDIDGTKVKVAVAHSCGQARALLDKVRDQI
                     AKDGKSEYQFIEVMACPGGCVGGGGQSWGSNIAKRARRGESLYEEDRSLPWRRSHENP
                     SVQALYEKYLEKPNSEKAHKLLHTHYYKRSTVDGKVLPNK"
BASE COUNT          599 a         1022 c          903 g          738 t
ORIGIN      
        1 aagatctcga gcatgcgctt cgtgcctatg cggcagggtg gacattttcc gcatgactca
       61 tgctggataa aatcgaggaa gaacctggcg aagtcgacca tgcacgtatc ctcgtcggca
      121 acgatgagac cgccagaccc gacgattgcg cccaactccg caacggattc atagtcgacg
      181 ggaacgttga gatggtcgat ggggatgcag ccgccggaag gaccgccgag ctgcgccgcc
      241 ttgtatttct tgcctccctt gatgccgccg ccgatgttga agacgatgtc tcccagcggt
      301 gtgcccatgg gcacttcgac gaggcccgta ttattgacgg cgccggcgag ggcgaaaacc
      361 ttggttccct tgctcttttc ggtaccatac tgggcgtacc aggcggctcc gttgttgatg
      421 atggcgggga tattcgcgta tgtttcaaca ttgttgagga gcgtcggggc gttgaaaaga
      481 cccttgacga cggggaaggg gggtctcggt ctcggttcgc cgcgcttgcc ctcgatggag
      541 atcatcaggg ccgtttcctc gccgcagaca aaagcgcccg cgccgatgcg gatctcgatg
      601 tcgaagttga aatccgtctc gaggatgttc ttgccgagga acttgtactc tctcgcttgt
      661 ccgatggccc agttcaggcg ctcgatggca aggggatatt ccgccctgac gtacacgtag
      721 ccctgctggg caccgatcgc gtaggcagct atggccatcg cctctatgac gctgtgcgga
      781 tcgccttcaa ggacgctgcg gtccatgaac gcgcccgggt cgccttcgtc ggcgttgcag
      841 acgacgtatt tcacgtcacc cggtgactga aaggcgaact tccatttcaa ccccgtcggg
      901 aatccgccgc cgccgcggcc acgcaggccc gacttcagca cttcgtcgat gacctcttcc
      961 cgggtcatcg aggttagcgc cttccccagg gccaggtaac cgtcccgggc gatgtactcc
     1021 tcgatgttca tcgggttgat gacaccacag tttctcagaa cgatcttgat ctggttcttg
     1081 aagaaatcta tctccttcat cgaagggatg gtcttggcag ataccgcctc cttgtaaaga
     1141 aggcgctcga ctatcctgcc cttgagaaga tgctccgtta ctatctcttc ggcgtccgca
     1201 ggcgtcagtt tctgatagag aaccccttcc gggtagacca gcgccagagg acccaggtca
     1261 caggagccga tgcaccccgt ttctatgacc ttgatctcat tctgcagacc ctgttcgaca
     1321 atcttattga caaccgcatc cctgatctga cggcaaccgg acgagacgca tgcagccccg
     1381 gcacaaacca atatgtgagc gcgagatacc tccatgttta tccttcctcc ttactcatac
     1441 ttttccagca catccatgag atgcggtggc ttgatccgcc cgtagacgtc ctcgttgacc
     1501 agcattgccg gcgcgaggcc gcatgcaccg atgcaacgca caacgtcgag ggaataccgc
     1561 ctgtcctccg ttgtgccgcc gaccttgatc ccgagttctc tctgggcctt gtccaggacc
     1621 tcctgggaac ctctcacgta acatgccgtg ccgagacaga ccttcacgct gttgcgtccc
     1681 tggggtacgg tagagaagaa attatagaag gtgatgacgc cgtacacctc gttgagcgaa
     1741 acgttgaggc cttcggcgac catctcctgg gcctttctcg gaagataacc gaacaggttc
     1801 tgcgccttgt gcaggaccat gataaggtct tctcccttcc cccgatgctc catgatgact
     1861 tcctcgagcc ggggatagag ctcttcctcc tgaacctcgg cacagtggca ttgcttttct
     1921 gacatcttgc tccctccctc ttagagtaac ctggcgatcg aacttacttg ttcggcaaaa
     1981 ccttgccgtc cacggtactc cgtttatagt aatgagtatg gagaagtttg tgggccttct
     2041 ccgaatttgg tttctccaga tatttctcgt acagcgcctg tacggagggg ttctcgtggg
     2101 aacgcctcca gggcaggctg cggtcttcct cgtacaggga ctcacccctg cgtgcccgct
     2161 tcgcgatatt gctgccccag gactggcctc cgccgccgac gcatccgccg ggacaggcca
     2221 tgacctcgat gaactggtac tcggactttc catccttcgc gatctggtcc cttaccttgt
     2281 cgagaagcgc ccgtgcctgg ccgcaactgt gggccacggc gaccttcacc ttcgtcccat
     2341 cgatgtcgat ctcggcctct tttatcccct taagacccct gacgggcttt atctccacgt
     2401 cctccagttc ccgtcccgtg acgagacggt aggcactccg caccgcggct tccataacgc
     2461 ctcccgtggc gccgaagatg gtggccgcgc cggtgtaatc acccagaagg tcatcggcgg
     2521 gttcatcggg catttctctt atgtcgagtc ctgcctcacg catcatcctg cccagttccc
     2581 tcgtcgtgag gacgtaatcg acatccctgt gcccgcttgc gttcatctcg ggccgcaggc
     2641 actcgaactt cttcgcggtg cagggcatca cggaaacgga gactatcttc gcggggtcta
     2701 tcccttttgc ctcggcgtag taggtcttcg ccagggctcc gaacatctgc tgcggcgatt
     2761 tggccgtgga catatgaggg atcatgtcat ggaagaaggt ctccatgaac tttatccagc
     2821 ccgggctgca ggaggtgatg agaggcaggt tcttccccga ggtaagcctg ccgataagct
     2881 cggtaccctc ctccaggatg gtcaggtcgg cggtgaagtt cgtttccagg accgcgtcga
     2941 agccgagcct tctcaaagcg gcgtgcatct tcccgctcac cagcgagccc gtcggcatgt
     3001 cgaactcttc gccgagctgg accctcaccg cgggcgcctc ctgaacgacg acgaacttgt
     3061 cggggtcgtg gatcgcgtcc cacacctcat cgacggcgct cttctcgtac agggccccga
     3121 cggggcaatg aacgacgcac tgcccgcaat tgatgcagac gctgttgccg agaccggcat
     3181 tgaaggccgt cgtgatgacc gtgtcgaacc ccctgtggtt cacggccagg gcgttcacgg
     3241 tctgaaccct gctgcacacc tc
//
LOCUS       DAOQTR010000229         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_34029_length_3251_cov_3.067897, whole genome shotgun sequence.
ACCESSION   DAOQTR010000229 DAOQTR010000000
VERSION     DAOQTR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34029_length_3251_cov_3.067897"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKN85_06395"
     CDS             complement(<1..874)
                     /locus_tag="PKN85_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT44072.1"
                     /translation="MKGVPFSYSLRNLWTRRLTTVLTVSGMALVVFVFTTILMLAEGL
                     QKTLVETGSYDNAVIIRKGSGTEVQSWVDRAQAGIIETDPIVATGPCGKRLVAKELVV
                     LIGLPKKDTNKVSNVTIRGIGESSLALRPQVALVRGRMPRMGSSEVVVGSSVAKRFKN
                     ADLGGELRFGMRQWRIVGVFDAGNTGFTSEIWGDVEQLMPAFRRPVFSSMLLRMNDPS
                     RFDDFKKRIETDPRLTQEVKRETRYYLEQSEVMARFLRILGISLTLIFSLGAIIGAMI
                     TMYAAVANRIGEIGT"
     gene            complement(871..2028)
                     /locus_tag="PKN85_06400"
     CDS             complement(871..2028)
                     /locus_tag="PKN85_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT44073.1"
                     /translation="MQILKILFKNAFRHKLRTGLTVLSITIALLAFGLLRTVINAWYS
                     GVEASSAYRLVTRNAVSIMFPLPYSYKDRIRQVDGVSEVSWGNWFGGIYIDEKNFFAN
                     FAVDARTYFDLYREYAIPDDERTAFFRDRKGFVAGRKLAERFGWKIGDTVVLKGTIYP
                     GNWEFVMRGIYRGREPNADETVFMFHWDYLNESLKKTAPRRADQVGFYMVGIEDPGRA
                     ISIAEAIDALFKNSFAETITETEKAFTLGFVAMSDTIITAIQLVSFVVIVIILAVVAN
                     TVAMSVRERTNEYAVFKTLGFGGWRIVGLILGESVVITTMGCAVGIAATYPAAALFGK
                     AMGAYFPVFNVEPDTIVLDIVFSLAVGVLAAVIPVYRTLKMKIADGLRMIE"
     gene            complement(2003..2704)
                     /locus_tag="PKN85_06405"
     CDS             complement(2003..2704)
                     /locus_tag="PKN85_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44074.1"
                     /translation="MDAGTIVSIQKLCKSYHRGNQVIPVLSDISFDIAEGEFLALMGP
                     SGSGKSTLLNLIAGIDQPDSGSIVIGDVDVTTLAETELAKWRALNVGFIFQFYNLIPV
                     LTAFENVELPLLLTPLSRSERKAHVEMALRVVGLADRMDHYPSQLSGGQEQRVAIARA
                     IVTDPTILVADEPTGDLDRNSAEEIMDLMDRLNEESKKTIIMVTHDPRAAKKAHVIRH
                     LDKGILTNADPQDTL"
     gene            complement(2705..>3251)
                     /locus_tag="PKN85_06410"
     CDS             complement(2705..>3251)
                     /locus_tag="PKN85_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT44075.1"
                     /translation="IITPIGASSTAKAAVVTLADMSSLKVEADVSESNLGKLKVGQPC
                     QIQLDAIPDTRFDGTVHMIVPTADRTKATVMVKVNFNHLDPRILPEMSAKVAFLSREV
                     GGDERRPRTAVSGKAVVERKGGKYLFVVKDGRADMREVTTGEVFGDMVEIKSGASSGD
                     RVVLDPPERLKDGSRVKITGQ"
BASE COUNT          610 a         1003 c          972 g          666 t
ORIGIN      
        1 aggtgccgat ctcgcctatc ctgttcgcca cggcggcgta catggtgatc atggccccta
       61 tgatggcccc gagggagaag atgagcgtca gcgatattcc gaggatgcgc aggaacctgg
      121 ccatcacctc tgactgttcc aggtagtagc gggtctcccg tttcacctcc tgtgtgagac
      181 gggggtccgt ctcgatcctt ttcttgaagt cgtcgaaccg cgaggggtcg ttcatccgaa
      241 ggagcatgga ggagaaaacg ggccgtctga aggcgggcat gagttgttcc acgtcccccc
      301 atatctcgga ggtgaaaccc gtgttgcccg cgtcgaaaac ccctacgatg cgccattgcc
      361 gcatcccgaa gcgcagctcg cctcccaggt cagcgttctt gaagcgtttc gcgacgcttg
      421 agccgacgac gacctccgag gagcccatcc ggggcatccg gcccctcacg agggccacct
      481 ggggacgaag ggcaagggag ctttccccga tcccccggat cgtcacgttg gagaccttgt
      541 tcgtgtcctt cttgggaaga ccgatgagga caacgagttc cttcgccacg agtcgctttc
      601 cacagggccc cgttgcgacg atgggatccg tttcgatgat gcccgcctgg gcacgatcca
      661 cccagctctg cacctccgta cccgacccct tccgtatgat gacggcatta tcgtacgacc
      721 cggtttcgac cagggtcttc tggaggccct cggcgagcat gaggatcgtc gtgaacacga
      781 aaacgacgag ggccatgccc gagacggtca gcaccgtcgt gagcctgcgg gtccagaggt
      841 tcctcaagct gtaggaaaag ggaacgcctt tcattcgatc atcctcaacc cgtcggcgat
      901 cttcatcttc agggtgcggt atacgggtat cacggccgcg aggacgccca cagccaggga
      961 aaaaacgata tcgaggacaa tggtatcggg ttcgacgttg aaaacgggga agtaggcccc
     1021 catcgccttg ccgaataacg cagccgcggg gtacgtggcc gcgatcccca cggcgcatcc
     1081 catcgttgtg atgaccacgg actctcccag gataagtccc actatccgcc agccgccgaa
     1141 acccagcgtc ttgaaaacgg cgtactcgtt cgtgcgctcc ctgaccgaca tggccaccgt
     1201 gttcgcgacg acggcaagga tgatgacgat gacgacaaag gaaacgagct ggatcgccgt
     1261 tatgatcgtg tccgacatgg ccacgaaacc cagtgtgaag gccttttccg tttccgtgat
     1321 ggtctcggcg aaggagttct tgaaaagggc gtcgatggcc tcggcgatgg agatcgcccg
     1381 gccggggtcc tcgataccca ccatgtagaa gcccacctgg tcggccctcc tcggggcggt
     1441 cttcttcagg gactcgttga ggtagtccca gtggaacatg aagaccgtct cgtccgcgtt
     1501 cggctccctc cccctgtata tgccgcgcat aacgaattcc cagttgccgg gatagatggt
     1561 gcccttgagg acgacggtgt cccctatctt ccatccgaat ctctccgcaa gtttccgtcc
     1621 cgcgacgaac cccttgcggt cccggaagaa ggccgtcctc tcgtcatcgg ggatagcata
     1681 ctcacggtag aggtcgaaat acgtccttgc gtcgaccgca aaattggcaa agaagttctt
     1741 ctcgtcgata tagatcccgc cgaaccagtt cccccaggac acctccgaga ctccgtccac
     1801 ctgcctgatc ctgtccttgt aagaataggg aagggggaac atgatggaga ccgcgttgcg
     1861 cgtcacgagc ctgtaggcgc tcgaggcctc gacccccgaa taccaggcgt tgatgacggt
     1921 cctcaggagc ccgaacgcaa ggagggcgat ggtgatggaa agaacggtaa gaccggtgcg
     1981 gagcttgtgc ctgaaggcgt tcttaaagag tatcttgagg atctgcattg gtcaggatgc
     2041 ccttgtcgag gtgcctgatg acgtgcgcct tcttcgccgc ccgcggatcg tgggtgacca
     2101 tgatgatggt cttcttcgac tcctcgttca atcggtccat gaggtccatg atctcctcgg
     2161 cggagttcct gtcgaggtcc cccgttggtt cgtcggcaac gagtatggtg gggtcggtga
     2221 cgatcgccct cgctatcgcg acgcgctgct cctgcccgcc cgagagttgc gacgggtagt
     2281 ggtccatccg gtcggcaagt ccaaccaccc tcaaggccat ctcgacatgc gccttgcgct
     2341 cggaacgtga aaggggtgtc agcaggaggg gcagttcgac gttctcgaag gccgtcagca
     2401 cggggatgag gttgtagaac tggaagatga aacccacgtt gagagcacgc catttcgcca
     2461 gctccgtttc cgcgagcgtc gtgacgtcga cgtcccctat cacgatggaa ccgctgtcgg
     2521 gctggtcgat gccggcaatg agattgagga gcgtgctctt gcccgaaccc gagggaccca
     2581 tgagcgccag gaactcccct tcggcgatgt cgaagctgat gtcgctcaag accgggatga
     2641 cctggttccc ccggtgatag gacttgcaga gcttttgtat ggagactatc gtcccggcgt
     2701 ccatctactg ccctgttatc ttcacccggg agccgtcctt gaggcgctcg gggggatcga
     2761 ggacaaccct gtcgcccgag gaggcgccgc ttttgatctc caccatgtcg ccgaagacct
     2821 ctcccgttgt cacttctctc atgtcggcgc gcccatcctt cacgacgaag agatatttcc
     2881 cgcccttcct ctcgacgacg gccttgccgc tcaccgccgt ccgcggtctc ctctcgtcgc
     2941 ctccgacctc ccgtgacaga aaggcgacct tcgcgctcat ctcggggagt atcctcggat
     3001 cgaggtggtt gaagttcacc ttcaccatga ccgtcgcctt cgtcctgtcc gccgtgggga
     3061 cgatcatgtg gacggtgccg tcgaaacgcg tatcgggaat tgcgtcgagc tggatctggc
     3121 agggctgtcc gaccttcagt tttcccaggt tcgactcgga aacgtccgcc tccaccttca
     3181 gcgacgacat gtccgcgagg gtgacgaccg ccgccttggc ggtggacgag gcgcctatgg
     3241 gggtgatgat g
//
LOCUS       DAOQTR010000230         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_34347_length_3231_cov_3.797544, whole genome shotgun sequence.
ACCESSION   DAOQTR010000230 DAOQTR010000000
VERSION     DAOQTR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34347_length_3231_cov_3.797544"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..586)
                     /locus_tag="PKN85_06415"
     CDS             complement(83..586)
                     /locus_tag="PKN85_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44076.1"
                     /translation="MNGRLPAYKKSFFLSPERPDGLQLQIFHEDGIVYSDLFIDNRFE
                     GYAEVLHGGMIFGILDVIVWYAIVMRTKIVAMTRKAEMEFFKLIMCSTPYRAKAEMLR
                     IEDRDIISTAWIEDSAGEVYARLNAVFREARGLAMDAFIDRFDFSTTTPAIKEYFLSL
                     LETDQGA"
     gene            complement(583..1671)
                     /gene="rlmN"
                     /locus_tag="PKN85_06420"
     CDS             complement(583..1671)
                     /gene="rlmN"
                     /locus_tag="PKN85_06420"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNT44077.1"
                     /translation="MEEFFGLTLSRLEAQIGALGKEKYRARQLFRWVYNQGNLDFREM
                     TNIPKSLRSVFSEMFSLDTMPIAEERSSRDGSTKLALVTRDAHVIEVVIMPEKSRVTL
                     CVSTQIGCRMGCRFCVTGKIGFKRNLSAAEIVGQVITAREYLRKAGKSPVTNIVFMGM
                     GEPMDNIDNVATALDILKDQSGLDLSYRKITLSSVGLVDGLKTLSAKSAVIAISLNAA
                     DDETRSMLMPINRLYSIGRIMDFARGFKGTKRTRITFEYILIKGVNDSLDDAKRLADL
                     LTGVKCKINLIPYNPSSYTEFERPERGEVDRFHQYLIERYFTVIIRDSRGQDVGGACG
                     QLGMGYLGGAPDPAAPDTKETTASEVTS"
     gene            complement(1763..2695)
                     /locus_tag="PKN85_06425"
     CDS             complement(1763..2695)
                     /locus_tag="PKN85_06425"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNT44078.1"
                     /translation="MKTATGTVAALLILLVLGVGLHASPPGAVYVNYIEGPVEHVAAN
                     SVRGVPAKANRALAEGESLVVAGPGNAELFIRDGSVLRIAGGSRLKVLSVERGAVHFL
                     LESGKAHVNFKGLKGYPLFFSTPSAQVDVFDRATFRVDIGGAGETEVSVLAGELYVAQ
                     PKGRMKIIVGTRLVMRKDGKTPVYTTNRPADEWDKWNKTRDGATELPSSGGSEPRRST
                     VYPAPAGSDPGAAGVAVPPETIPTPVVTHEYIYVGVGPAWGYSWYPYPYPYRPYAWHW
                     WGPRYPGWSGHRRWHGPPRGYWGHRSHPFRRHWR"
BASE COUNT          575 a         1001 c          985 g          670 t
ORIGIN      
        1 ccgtgtggtt agtataccgc aactttggtg tccactaaag gcatcatagg tcaaggcctc
       61 catcttctag cgctcaagcg gttcaagctc cttgatcggt ttccaggagc gacaggaagt
      121 attcctttat cgccggcgtc gttgtcgaga agtcaaagcg gtctatgaag gcgtccatgg
      181 caaggcccct ggcttcgcgg aagacggcgt tcagccgcgc gtagacctcg cccgcggagt
      241 cctctatcca ggccgtggag atgatgtccc tgtcctcgat cctcagcatc tcggccttcg
      301 cgcggtaggg ggtactgcac atgataagct taaagaactc catctccgcc tttctcgtca
      361 tggcgacgat cttcgtcctc atgacgatgg cataccagac gatgacgtcg aggatgccga
      421 atatcatgcc gccgtggagg acctcggcgt aaccctcaaa gcgattatct atgaagaggt
      481 cggagtagac gatgccgtcc tcgtggaata tctggagctg gagcccgtcc ggcctctcgg
      541 ggctcaggaa gaaggatttc ttgtacgccg gaagtctgcc gttcatgatg tcacctcgga
      601 agctgtcgtc tctttcgtgt cgggggcagc ggggtccggg gcacctccga ggtagcccat
      661 gccgagctgc ccgcatgccc cgccgacgtc ctgcccgcgg gaatcgcgga tgatgaccgt
      721 gaagtaccgc tcgatgagat actggtggaa cctgtccacc tcacccctct ccggtctctc
      781 aaattcggtg tacgacgaag ggttgtaggg tataaggttg atcttgcact tcacgcccgt
      841 gaggagatcc gcgagcctct tcgcgtcgtc gagggaatcg ttgaccccct tgatgaggat
      901 gtactcgaag gtgatcctcg tccgtttcgt ccccttgaag ccccgcgcga aatccatgat
      961 cctgccgatg gagtaaagcc tgttgatggg catcagcatg gaccgcgtct catcgtcggc
     1021 ggcattgagg gagatggcga taacggcgga cttcgcgctg agcgtcttga gcccgtcgac
     1081 aagcccgacg gaggaaagag tgatcttgcg gtaggaaagg tcgaggccgc tctggtcctt
     1141 caggatgtcg agggctgtgg cgacgttgtc gatgttgtcc atgggttcgc ccatccccat
     1201 gaagacaata ttcgtcacgg ggctctttcc ggccttcctc aggtattccc gagcggtgat
     1261 gacctgtccc actatctccg ccgcgctgag gttcctcttg aagcctatct tgcccgtcac
     1321 gcagaacctg catcccatgc ggcagccgat ctgcgtggag acacagagag tgacgcgact
     1381 cttctcgggc atgatgacga cttcgatgac gtgcgcgtcc ctcgtcacga gggcgagctt
     1441 cgtcgagccg tcccgggagg acctctcttc ggctatgggc atggtatcga gagagaacat
     1501 ctccgagaaa acactcctca agctcttggg gatgttcgtc atctcccgga aatcgaggtt
     1561 accctggttg tagacccagc ggaaaagctg gcgggcacgg tatttctcct tgcccagcgc
     1621 tcctatctgc gcctcgagac gggaaagggt gagaccaaaa aactcttcca ttgcttgact
     1681 gactctccgg tgcgggtcct tgacgaacgc gaaacaccat acgataccag aaaaagctca
     1741 ttctttcaag atattcagag ggttaacgcc aatggcggcg gaaaggatgg ctgcggtgac
     1801 cccaataacc ccggggagga ccatgccacc gccggtggcc cgaccatcca ggatatcggg
     1861 gaccccacca gtgccacgca taggggcgat agggataggg gtagggatac caggaatagc
     1921 cccatgccgg gcctactccg acatagatgt actcatgcgt aacgacgggt gtggggatcg
     1981 tttcgggagg gaccgcgacc cctgcggcac ccgggtcgga gccggccggt gccgggtaca
     2041 cggtcgaccg ccggggttcg ctcccgccgc tcgagggaag ttctgtcgcc ccgtccctcg
     2101 tcttgttcca tttatcccat tcgtccgcgg gcctgttcgt ggtgtaaacg ggggtcttgc
     2161 catccttcct catgacgagg cgcgtcccga cgatgatctt catcctgcct ttcggctggg
     2221 cgacatagag ctcgccggca aggaccgaca cctccgtttc tcccgccccg ccgatatcca
     2281 cgcggaaggt agcccggtcg aacacgtcga cctgggcgga gggcgtgctg aagaagaggg
     2341 gatatccctt gagccccttg aagttgacat gggccttccc gctttccagc aggaaatgga
     2401 cggcgccgcg ctcgacggac aggaccttga gccggctgcc cccggcgatc ctcaacacgc
     2461 tgccgtcgcg tatgaaaagc tccgcgttgc ccggccccgc caccacgagg gactcacctt
     2521 cggcaagggc cctgttggct ttggccggca cacccctcac tgagttcgcg gcaacgtgtt
     2581 cgacagggcc ttctatgtag ttcacgtaga cggcgccggg aggggaagcg tgcaggccca
     2641 caccgagcac caataggatg agaagcgccg ctaccgttcc tgttgctgtc ttcatgtcgc
     2701 ccctttcctg actatatcca ttctaacacc ttcttcgcga aaagtgcacg cggcatcaat
     2761 tcctcacctg cttcaccggg gaaccccgcc gcacatcagg ccccctaccg ttccctggcc
     2821 ccggccttga gacctcatcc ttgtcgggcg ccgccacagg ctgggcggaa ggtgccggcg
     2881 gggctgcggc cttttccttc accgggttct gcctggccgc ctgcggctca gccttttccc
     2941 ttggcaccgc ggccggcctc tgctcgacgc ggggtgttgc ctggcggggc gcctccggta
     3001 ctctcacggc gggttgtgcc ggcctctgaa cgttctgggc cgccggcgca gttacggctg
     3061 caggcctcgc ctgcacgggc gcgctcaccg acggggtagg ggtaggggta ggggtagggg
     3121 taggggtgtt gcgcggtgcc acgggccggc gattctctgg aacacgcact gtcgggctac
     3181 tctgggtgcc actcaccgcc acggttgatg accgttctgc cgtccttcca g
//
LOCUS       DAOQTR010000231         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_34400_length_3228_cov_3.667822, whole genome shotgun sequence.
ACCESSION   DAOQTR010000231 DAOQTR010000000
VERSION     DAOQTR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34400_length_3228_cov_3.667822"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="bioD"
                     /locus_tag="PKN85_06430"
     CDS             complement(<1..690)
                     /gene="bioD"
                     /locus_tag="PKN85_06430"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNT44079.1"
                     /translation="MKSVFVTGTDTGVGKTIISSSIAAYLSTVKGLDVGVMKPFESGI
                     ETAGDRGLDDTWILKTASGSEDDVNEINPYLFKAPLAPEPAAAEENASIDIDLVTSLY
                     DRLAARHDVVIVEGAGGLLVPITERFFYADLIRAWRAPVIVVSRLTLGTINHTLLTCG
                     HLRSIGAKVLGVILNDTEGAGDIASRTNPAMLKKYLDVPLLGVFPHTPDLFGKGVNRE
                     RLADLLARNIDC"
     gene            complement(690..899)
                     /locus_tag="PKN85_06435"
     CDS             complement(690..899)
                     /locus_tag="PKN85_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44080.1"
                     /translation="MWKVLANAVEMLIYAAVYIILALVAVKVIGATFTTDFEKKISEE
                     NNFALALICASLFTGLAILLSAIVR"
     gene            complement(912..1553)
                     /locus_tag="PKN85_06440"
     CDS             complement(912..1553)
                     /locus_tag="PKN85_06440"
                     /inference="COORDINATES: protein motif:HMM:NF003989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNT44081.1"
                     /translation="MAKFSKIPEATIRRLSSYLKCMVDLEGKSEKVASSAVIASICNV
                     NAAQVRKDFAYFGEFGIRGMGYNVKDLKRHIKEILGINREWRIAVVGVGNMGSALLVY
                     KDFLKQNYKIVAAFDLYPEQVIGRISERVGKPVEILHLNRLREVVQARNIEIGIITTP
                     PQEAQKVADLLIDANIKGILNFSPAPVRTSGEVKLRNLFFTSALDNLVYYLSN"
     gene            complement(1825..2610)
                     /gene="dapB"
                     /locus_tag="PKN85_06445"
     CDS             complement(1825..2610)
                     /gene="dapB"
                     /locus_tag="PKN85_06445"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNT44082.1"
                     /translation="MARVVITGACGKMGRAIVKLALEDPDLEIAGLVEVKGHPMTGQA
                     HDLMGGRAPFVSDEMPQVLTGADVVVDFTEAEASVNHFRMAAEKGVAHVIGTTGLKED
                     HMRTIREAKGARIVISPNMSIGMNVLFDLAQRVSSVLGDAYDAEIVELHHRWKKDAPS
                     GSALRIKDAVEAGQGTRAWIEVFGREGITGERKKDEIGVMSIRGGDIVGEHTLYFAGI
                     GERLELTHKAWSRDNFARGALIAAKWLIKQPPGIYSMKDVLGL"
     gene            complement(2635..3207)
                     /gene="dapA"
                     /locus_tag="PKN85_06450"
     CDS             complement(2635..3207)
                     /gene="dapA"
                     /locus_tag="PKN85_06450"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNT44083.1"
                     /translation="MKRDGLLAHFTTIAEKTRIPLVLYNIPGRTGINMAPETIAELAK
                     VRNIVGIKEAAGSLTQVSEIYRLTKGRFAILSGDDNLFLPMMSVGAVGVISVISNIMP
                     KELKSLYKAFMVEKNIAKAIRIHTKLMPLFQGMFIETNPIPVKEAAYRMGMIAREYRL
                     PLCPMSDKNAAFLGGLLGEYGLAGKGRRKG"
BASE COUNT          631 a          952 c          894 g          751 t
ORIGIN      
        1 gcagtcgatg tttcttgcca gaaggtccgc gagtctttcc ctgtttacgc ccttcccgaa
       61 gaggtcaggc gtgtggggga aaacccccag gagggggacg tcgaggtatt tcttcagcat
      121 ggcggggttc gtccgcgagg ctatatcgcc cgcaccttcc gtgtcgttga ggatcacgcc
      181 gagcaccttc gcgccgatgg acctcaggtg gccgcatgtc aggagggtgt ggttgatggt
      241 ccccagggta agcctcgaca cgacgatgac gggggccctc catgcccgga tgaggtcggc
      301 atagaagaag cgttccgtga tagggacgag aagcccaccc gcgccctcga cgatgaccac
      361 gtcatggcgg gccgcgaggc gatcgtacaa tgatgtgacg agatcgatgt cgatggaagc
      421 gttctcctcg gcggcagccg gttcaggcgc gagcggcgcc ttgaagaggt aggggtttat
      481 ctcgttgaca tcgtcttccg agcctgacgc cgttttgagg atccacgtgt cgtcgaggcc
      541 cctgtcgccc gctgtttcga tgccgctttc gaagggcttc atgaccccca catcgaggcc
      601 tttcactgtc gagaggtagg cggcaatcga cgaggagatg atggtcttcc ccacaccggt
      661 atccgtaccc gttacaaaaa cacttttcat catctcacta tggcggagag aaggatcgca
      721 agacccgtaa agagggacgc gcatatgagg gccagcgcga agttgttctc ctcggagatc
      781 ttcttttcaa aatcggtggt gaaggtagcg ccgatcactt tcacggcaac gagggcgagt
      841 atgatataca ccgccgcata gatgagcatc tccaccgcat ttgcgagaac cttccacatg
      901 gtcgcctctg gctagttcga caaataatat accagattgt ccagcgcgga tgtgaagaaa
      961 agatttctca gtttcacctc ccccgaggtc cgcacgggtg ccggcgagaa gttgagaatg
     1021 cccttgatgt tcgcatcgat gagcagatcg gcgaccttct gggcctcctg cggaggcgtc
     1081 gttatgatgc cgatctctat gttgcgggcc tgcacgacct ctctgaggcg attgagatgc
     1141 aggatctcca ccggtttgcc cacgcgctcg gagatacgcc cgatcacctg ttccggataa
     1201 agatcaaagg cggcaacgat cttgtagttc tgtttgagga aatccttata gaccagcagg
     1261 gcgcttccca tgttaccgac accgacgacg gctatgcgcc attcccggtt gatccccagt
     1321 atctctttga tgtggcgctt caggtccttc acgttgtagc ccatgccgcg gatgccgaat
     1381 tctccgaagt aggcgaaatc ttttcgcacc tgcgccgcgt tgacattgca gatgctggcg
     1441 atcacggcgc tcgaggcgac cttttcgctc ttgccctcga gatcgaccat acacttgaga
     1501 tagctcgaaa gccttcgtat ggtcgcttcg ggaatctttg agaattttgc cattgtccct
     1561 taccttctct gtctgaattt tcccttttgt ttcctatatg taacagttcc aagggggttt
     1621 tgcaagagaa aaatttaaca agcaaactcc cccgcgcgca cccgcgttat acctgatttc
     1681 acaggggata aggtgttgga gattaaatgt taagtcttaa gttgaaagac ctcggatatc
     1741 cgcacgccgc ccccctcata cgtttcgtgc tatacaatgt ctccaatagc ttgaaactca
     1801 aaacctaaaa cataaaacta tttttcacag ccccaggacg tccttcatgg agtagatccc
     1861 cgggggttgt ttgatgagcc acttggccgc gatgagcgct cccctggcga agttatcccg
     1921 cgaccaggcc ttgtgggtca gctcgagccg ctcgccgata ccggcaaaat aaagggtatg
     1981 ctccccgacg atatccccac cgcggatgct catgacaccg atctcgtcct tcttgcgctc
     2041 gcctgtgatc ccctcgcgcc cgaagacctc gatccaggcg cgggttccct gtcctgcttc
     2101 gacggcgtcc ttgatcctca gggccgaacc gctgggggcg tccttcttcc accgatggtg
     2161 gagttcgacg atctcggcat cgtaggcgtc tcccaggacg gaggaaaccc tttgggccag
     2221 gtcgaagagg acgttcatgc cgatgctcat attcggagag ataacgatcc gggcgccttt
     2281 tgcctccctg atcgtcctca tgtggtcctc tttcaggccc gtcgtgccga tgacgtgggc
     2341 cacgcccttt tcggccgcca tcctgaaatg gttgacagag gcctccgcct ccgtgaaatc
     2401 gacgaccacg tccgccccgg tcaacacctg cggcatctcg tcgctgacaa agggggctct
     2461 tccgcccatg aggtcgtgcg cctgtcccgt catgggatgg cctttcacct cgacgaggcc
     2521 ggctatctca aggtccgggt cctcgagggc gagctttact atggcccgcc ccatttttcc
     2581 gcatgctcct gttatgacaa ctcttgccat accacagacc tcccggttcc ggggctaacc
     2641 cttgcgtcgg cctttacccg cgaggccgta ctcgccgaga agaccaccga ggaacgcggc
     2701 gttcttgtcg ctcatggggc aaaggggcag gcggtactcg cgcgcgatca ttcccatccg
     2761 gtaggccgcc tccttgacgg gtatggggtt cgtttctatg aacatgccct ggaaaagggg
     2821 catcagcttc gtgtgtatcc tgatggcctt cgctatgttc ttctccacca tgaaggcctt
     2881 gtacagggac ttgagttctt tcggcatgat attggagatg accgatatga cgcccaccgc
     2941 cccgacgctc atcatgggga ggaaaaggtt gtcgtctccg gagaggatcg cgaatctgcc
     3001 ctttgtcagg cgatagatct ccgagacctg cgtcaacgac cccgcggcct ccttgatgcc
     3061 aacgatgttc cgtaccttcg ccagttcggc tatcgtttcc ggggccatgt ttatgcccgt
     3121 ccggcccggt atgttataga gaacgagggg gatccgggtc ttttccgcta tcgtcgtgaa
     3181 atgtgccaga aggccatcac gtttcacgca tcacgtttta ccccaaaa
//
LOCUS       DAOQTR010000232         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_34749_length_3207_cov_3.752538, whole genome shotgun sequence.
ACCESSION   DAOQTR010000232 DAOQTR010000000
VERSION     DAOQTR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34749_length_3207_cov_3.752538"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1048)
                     /locus_tag="PKN85_06455"
     CDS             complement(59..1048)
                     /locus_tag="PKN85_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44084.1"
                     /translation="MITGIGSFPFTSVDETIDLIFSTCKGIPFWPQLPKRSLLENMYT
                     TFLEGVPCIVSDEEKNTIFMNTEGTDGIEEFYENVANDNVGAFAISERYAPGFHRFLE
                     RLREIEDGLQFIKCQVTGPFSMGLGLKDENDKPVIYNFAFYDIIKKALHMKAKWMIKT
                     IQSAYPGVDIIIFFDEPYMVSFGSAYISIPREEVIASLNEVVEDLPARRGIHVCGNTD
                     WSVPFQSDIDIVNYDAFSYLDTVFYFNEDLKEFLDRGGWIAPGVVPSSPEVMQETPDT
                     VRDRAASFVEKIASVTAPDMDSILFTTSCGLGSLTEEEARRAMELLRQIAYGE"
     gene            1295..2593
                     /locus_tag="PKN85_06460"
     CDS             1295..2593
                     /locus_tag="PKN85_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNT44085.1"
                     /translation="MTIYNGVSAAGVLCFLGFLWLLSRHRRKVSLRVVVWGVALQIVF
                     ALFVFLVPFGSRLFLILNDITMEVIESAFEGIRFLFGPLALSPGKAGSPGFILAFQAL
                     PSVIFFAALMELLYYMGIMERLIRLFAKVFVKVMGISGAESLCASAQIFLGIESNLTI
                     RPYLKEMTPSELMVVLTTGMATIASSVLGFYVIILSGALPKVAGHLISASILLAPAAI
                     LASKVLFPETGSPRTLGKVVAVDYQKAANPIEAVINGAMTGVKMLVGIGALLIAFIGI
                     VALFDSGLHYLGKLIGSAAGTTVDLSLNHLLGYIFYPFTVAMGVPLDDAPQIARIIGE
                     RIIMTEVKSYQDLSTLVQAGKIVHERSVFIATYALCGFAHIPSLAIFVGGTSSLVPER
                     TKDIAVLGLWALLAANVACFITGAVAGIFYTGTGGILFRP"
     gene            complement(3004..>3207)
                     /locus_tag="PKN85_06465"
     CDS             complement(3004..>3207)
                     /locus_tag="PKN85_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44086.1"
                     /translation="GGDYFRKEIIGTVIGAFTPFYAFGAIGAHWFGGYVRDVTGSFSI
                     PFMVAIGLAATSAVLMGFVKKEG"
BASE COUNT          634 a         1009 c          862 g          702 t
ORIGIN      
        1 tctggttatc agctcatcgc cctatcacct atgacccatc acccatgacc cgcctctttt
       61 actccccgta cgcgatctgc ctcagcagct ccatggcccg gcgggcctct tcctcggtga
      121 ggcttccgag gccgcagctg gtggtgaaga ggatgctgtc catgtcgggg gcggtgacgg
      181 acgcgatctt ttccacgaag gacgcggcac ggtcccgcac cgtgtcgggc gtctcctgca
      241 tgacctccgg cgacgaaggg acgacacccg gcgcgatcca gcctcccctg tcgagaaact
      301 ccttgaggtc ctcgttaaag tagaagaccg tatcaaggta ggagaaggcg tcatagttca
      361 cgatgtcgat gtccgactgg aaagggacgg accagtccgt gttgccgcac acgtgaatgc
      421 cgcggcgggc gggcaggtct tcgacgacct cgttcaggga ggctatgacc tcctcccggg
      481 gaatggatat gtaggcggac ccgaaggaga ccatgtacgg ttcgtcaaag aagatgatga
      541 tatccacgcc ggggtatgcc gactggatgg tctttatcat ccacttcgcc ttcatgtgga
      601 gggccttctt gatgatgtcg tagaacgcga aattgtagat gaccggcttg tcgttctcat
      661 ccttgaggcc aaggcccatg ctgaacggtc ccgtcacctg gcacttgatg aactgaaggc
      721 cgtcctctat ctcgcgaagc ctctcgagaa agcggtggaa accgggtgcg tatctttccg
      781 atatggcgaa ggccccgacg ttgtcgttcg caacgttctc gtagaattcc tcgatcccgt
      841 cggtcccctc ggtgttcatg aagatggtgt tcttctcttc gtcgcttacg atgcagggca
      901 caccctccag gaaggtggtg tacatgttct ccagcaggga acgcttcggc agttgcggcc
      961 agaagggtat ccccttgcag gtggaaaaaa tgagatcgat cgtttcatcg acactcgtaa
     1021 aaggaaaact gccgataccg gttatcatta tctcctcgta agtggacttt gcactgctat
     1081 ctggggaaat ttctatcaca cgtggcggga tgaagtaaag agaagagggg gaaaggagag
     1141 ggaagaagca gataggacct ataggaatca ataagaccta taggtcctat caaaacccct
     1201 cccctgccgc ctcatcccct cacctctctt cttgtcaatc cccgccatac ctgttatctt
     1261 atccgcacaa tatcacaata caggggatga tcgggtgacg atatacaatg gggtttccgc
     1321 ggcgggtgtg ctctgttttc tcgggttcct gtggcttctc tcgcggcaca ggcgaaaggt
     1381 gagcctccgg gtggtcgtct ggggggtggc gctccagatc gtcttcgctc ttttcgtctt
     1441 tctcgtccct ttcggttcga ggctctttct catcctcaac gacataacca tggaggtcat
     1501 agagagcgcc ttcgagggga tcaggttcct cttcggcccc ctggcgctgt ctccgggcaa
     1561 ggccggctcg ccggggttca tcctcgcctt ccaggcgctg ccttccgtca tattttttgc
     1621 cgccctcatg gagctcctgt actacatggg gatcatggaa cgtctcatca ggcttttcgc
     1681 gaaggtcttc gtcaaggtca tgggcatcag cggcgctgag tcgctctgtg cctcggccca
     1741 gatattcctc ggcatcgaat cgaacctgac gatccggccc tacctcaagg agatgacccc
     1801 gtcggagctc atggttgtcc ttacgacggg catggccacc atcgcgtcga gcgttctcgg
     1861 cttctatgtc atcatccttt ccggcgccct gcccaaggtg gcgggccacc tcatctccgc
     1921 ctcgatcctg ctcgctcccg ccgccatact cgcgtcgaag gtcctctttc ccgaaacggg
     1981 ctcccccaga acactcggaa aggtcgtcgc cgtcgattac cagaaggccg ctaacccaat
     2041 agaggcggtc atcaacggcg ccatgaccgg cgtaaagatg ctcgtcggca tcggagcgct
     2101 ccttatcgcc ttcatcggca tcgtcgccct cttcgacagc ggcctccact acctcggcaa
     2161 actcatcggt tccgctgccg gcaccaccgt cgacctgtcc ctcaaccacc tcctcggcta
     2221 catcttctat cccttcaccg tggcaatggg cgtccctctc gacgacgccc cgcagatagc
     2281 ccgcatcatc ggtgagcgca tcatcatgac ggaggtgaaa tcctatcagg acctcagcac
     2341 cctcgtgcag gcgggaaaga tagtccacga aagaagcgtc ttcatcgcca cctacgccct
     2401 ctgcggattc gcccacatcc cgtcactcgc catcttcgtc ggcggaacgt cgagcctcgt
     2461 gcccgaacgc acgaaggaca tagccgtcct cggcctctgg gccctcctcg cggccaacgt
     2521 ggcctgcttc ataacgggcg ccgtggcggg catcttctac accggaaccg gcgggatatt
     2581 gttcagaccc taaaagatca aagacattat ctctcgcccg cttcgctcga gttcgcagag
     2641 gacgcggaga gagactgaaa agagagaaaa gattgccgcc ggatctcgga gcctcccaaa
     2701 atccggcggc cttctcgtcc cgccgtcccg gcgggagaga aagaaatagt ctattttatc
     2761 ctcaagaagt tattccccct gtcgtcatag gtcctatatg tccgataggt cctatgacca
     2821 tcctccaacc ccccagactt ccatccctcc cccacaactc ccacctgaaa ccaaacctct
     2881 tctttccctc ccgtcggaga cgggatgcaa agccggccgg attttggaag gcttcgaaat
     2941 ccggccgaat cttttctctg cgtcctctgc gaacttgagc gaagcgggcg agaggcaagg
     3001 cctttaccct tccttcttca cgaaccccat cagcaccgcc gatgttgccg cgaggcctat
     3061 ggcgaccatg aaggggatgg agaaggagcc cgtcacgtcg cgcacgtagc cgccgaacca
     3121 gtgggcgccg atcgcgccga aggcgtagaa gggtgtgaag gctccgatca ccgtgccgat
     3181 gatctctttc ctgaaatagt cgccgcc
//
LOCUS       DAOQTR010000233         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_34915_length_3196_cov_5.259153, whole genome shotgun sequence.
ACCESSION   DAOQTR010000233 DAOQTR010000000
VERSION     DAOQTR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34915_length_3196_cov_5.259153"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKN85_06470"
     CDS             complement(<1..610)
                     /locus_tag="PKN85_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44087.1"
                     /translation="MKRDAEPVVLKDLRNFPVVAILGPRQCGKSTLVKAMKGRIGALI
                     YLDLESPSDLRKLSDPELFFQANKDTTICLDEIQLRPELFPVLRSIVDRSGRKGQALI
                     LGSASRNLIRQGSESLAGRISFIELTPFVTSEVAGLPGYDLLVHWFRGGYPESFLADD
                     DDASNRWRQNFLRTFIERDIPQLGIRITARNLRRLITMLAHSQ"
     gene            complement(747..980)
                     /locus_tag="PKN85_06475"
     CDS             complement(747..980)
                     /locus_tag="PKN85_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44088.1"
                     /translation="MSTPIKETPILRGKDARRFTEKITRNEQEPAPREEYDKLMANYR
                     KVKTDKPLAAFAEGREKTFGAGEALTHEEDVWS"
     gene            complement(993..1748)
                     /locus_tag="PKN85_06480"
     CDS             complement(993..1748)
                     /locus_tag="PKN85_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNT44089.1"
                     /translation="MARNVNDMIKDLGFTYNPNDLSIELPEPDRDRAIFRVQKLFPEF
                     VWDTTALEGNPFTFPEVKTLLEGVTVGGHRIEDEAQVLNQARSWKRLVEMVRDGSFSV
                     DKATFCTLNGIVAREEALEWGTFRNGQVGIAGTSFKPPAHQELDSLFERGTAIVNGIS
                     NPVEKGMVFYLFGALNQFFYDGNKRTSRLMMNGVLLMNGHDAISVPARQRLEYNEKMI
                     RFYDTLDGTEMMSFLLDCSTLRSPAPNSTLTQG"
     gene            complement(1882..2211)
                     /locus_tag="PKN85_06485"
     CDS             complement(1882..2211)
                     /locus_tag="PKN85_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44090.1"
                     /translation="MSFPSMEEILAAKKRERERLRNLPFEEKIKIVEEMNKFLAPLHA
                     RLDKDNWDMPRRALVRGVRRHRSELWGKKWCYLFPETGKKFHFNTKEEGDAILDRELK
                     DGGLLAT"
     gene            complement(2222..2833)
                     /locus_tag="PKN85_06490"
     CDS             complement(2222..2833)
                     /locus_tag="PKN85_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44091.1"
                     /translation="MSIKREKVKLLIPDASPLISLAHGGEDCLELIFKAKLPVVMTDQ
                     VLFEATYRKDEFQDAAVIDKWYRKHRTRIKVVATKIGEARRALESVGKYDHKKFADQG
                     EESVLQAIKSKQLEPGPYLFLFEDEQTADPSFFGVYPVHVVSTYGFLVGLERAHVIPS
                     ADKILERIRSGGRHSLKADIIDRPTRKVRDPGEESEWVPTSPG"
     gene            complement(2823..>3196)
                     /locus_tag="PKN85_06495"
     CDS             complement(2823..>3196)
                     /locus_tag="PKN85_06495"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HNT44092.1"
                     /translation="YTLNLDDSLMMAAKSKALQDRTSVSEMVRRLLSEYLGLSDRTAD
                     NTPHEKEIIEILERYSSGQLKRSEAMRAIGLDPVELEAFTSLMSEYGMSWPEVDRERA
                     KKEGEYVARAIAFNSGEIDEY"
BASE COUNT          617 a          921 c          841 g          817 t
ORIGIN      
        1 cctggctgtg cgccagcatc gtgatgagcc ttctgaggtt ccgtgccgtt atgcggatcc
       61 ccaactgggg aatgtcccgt tcaatgaagg tgcgcaggaa gttctgtctc caccggttgc
      121 tcgcatcgtc gtcatcggcc aggaagctct ccgggtatcc tccccggaac cagtgcacca
      181 acagatcata gccgggcagg ccggcgacct ctgatgtcac gaagggcgtc aattcgatga
      241 aggatattct ccccgcaagg gattcagaac cctgacggat gaggtttctg gacgcggacc
      301 ccagtatgag ggcctgcccc tttcttcccg aacggtcaac aatactcctg agcaccggga
      361 atagctcggg tctcagctga atctcgtcga ggcatatggt ggtatccttg ttcgcctgga
      421 agaaaagttc gggatcgctc agtttgcgaa ggtctgacgg gctttcgagg tcgaggtaga
      481 ttaaggcgcc gatcctgccc ttcatcgctt ttaccagtgt ggacttaccg cattggcggg
      541 gaccgaggat ggcgaccacg gggaagttgc ggaggtcctt aagcacgaca ggttcggcat
      601 ctcttttcac ataaccatgc atattagaag tgtaacttct ataatgcatg attgcaagga
      661 gattctacga tcctgctgtc cgtttgtcga gcttcggcag gtcctcggtc tttacggatg
      721 gatggtacct gagcgtgtac ggcacgtcag ctccacacgt cctcctcatg cgtcagagct
      781 tctccggccc cgaaggtctt ctcccggcct tcggcgaatg cggcaagggg cttgtcggtc
      841 ttcaccttcc ggtaattcgc catcagcttg tcatactctt cccgcggggc cggctcctgc
      901 tcgttcctgg tgatcttctc tgtgaacctc ctggcatctt tgccgcggag gatcggggtt
      961 tctttgatgg gggtggacat gaaatacctt ccttatccct gggtgagcgt ggagttcggt
     1021 gccggggacc tgagtgttga acaatcgagc agaaatgaca tcatctccgt tccatcgagc
     1081 gtgtcgtaga agcgtatcat cttctcgttg tactcgaggc gctggcgagc cggtacgctg
     1141 atggcgtcat ggccattcat cagcagcacc ccgttcatca ttagcctcga tgtgcgtttg
     1201 ttaccgtcgt agaagaactg gttgagggcg ccgaacaggt agaagaccat gcctttttca
     1261 accggattgg atatcccgtt gacgatcgcc gttcctcgtt cgaagagaga gtccagttcc
     1321 tggtgggcgg gcggtttgaa gctcgtgccg gcaataccga cctgaccgtt acggaaggtg
     1381 ccccattcaa gggcctcttc ccgggcgacg ataccgttga gtgtgcagaa tgtcgccttg
     1441 tccaccgaga aactgccgtc cctgaccatt tccacgagtc gcttccagct ccttgcctgg
     1501 ttcagaacct gagcttcgtc ctcgatcctg tgtccgccca cggtgacacc ttccagcagt
     1561 gttttcacct cgggaaacgt gaacgggttg ccttccaagg cggttgtgtc ccacacgaac
     1621 tcagggaaca gtttctggac cctgaagatc gcccggtccc tgtcaggttc tgggagctca
     1681 atgctgagat cgttcgggtt gtacgtgaat ccgagatcct tgatcatgtc gttcacattc
     1741 ctggccatgt tttccgatcc ggtgatttga gaagcctata cattatacat agcaccggag
     1801 tgctgttcaa gatgcgctac cacgggaagt ggaggtcctt aagcgcgaca ggctcggcgt
     1861 ccctcgcatt cttggtcgtc ttcatgtggc aagaagacct ccgtctttta actctctgtc
     1921 caggatcgca tctccctctt ctttcgtgtt gaagtggaac ttcttccccg tttcggggaa
     1981 gagatagcac cactttttgc cccagagttc cgacctgtgc cgccgaacgc ctctcacaag
     2041 ggcccgtcgc ggcatgtccc aattgtcttt gtcgagcctc gcatgaagcg gcgccaggaa
     2101 cttgttcatc tcttccacga tctttatctt ctcctcgaaa ggaaggtttc tgagcctttc
     2161 gcgttcgcgc ttctttgcgg caagaatctc ttccatgctt gggaacgaca ttgcccctcc
     2221 gttatcctgg tgacgtcggg acccattccg attcttctcc gggatctcgg actttcctgg
     2281 tcggccggtc aataatgtcg gccttaaggc tgtgccggcc acccgatcgt atgcgttcaa
     2341 ggattttgtc cgccgagggt atgacatggg cccgttccag accaaccagg aacccgtagg
     2401 tagatacaac atgaaccgga tagacgccaa aaaaggaagg gtccgctgtc tgctcgtctt
     2461 cgaaaagaaa cagatacggc ccgggctcca gttgcttcga tttgatcgcc tgcagtactg
     2521 actcttctcc ctgatcggca aacttcttgt gatcatactt cccgacggac tccagcgctc
     2581 tccttgcttc gcctatcttt gtcgcaacga ccttaatcct cgtcctgtgt ttccggtacc
     2641 atttgtcaat aacagctgcg tcttgaaact catccttcct gtaggtggct tcaaaaagca
     2701 cctggtccgt catgacgaca gggagttttg ccttaaagat cagttcaagg caatcctctc
     2761 ccccgtgagc gaggctgata agcggggagg cgtccggtat aagcagcttg accttctccc
     2821 tcttaatact catcgatctc tcccgagttg aaggcgatgg cccgcgcaac gtattcgcct
     2881 tccttttttg ctcgctcacg atcgacctcc ggccatgaca ttccgtattc actcatcagg
     2941 gacgtaaagg cctcgagttc caccgggtca agaccgattg cccgcatcgc ctcggacctc
     3001 ttgagctgac ccgaggagta tctctcaaga atttcaataa tctctttttc atgaggtgtg
     3061 ttatcggccg tccggtcgct gaggcccagg tactcagaca gaagacgcct taccatctca
     3121 ctgacagacg tcctatcctg caaggccttc gatttcgccg ccatcatcag ggagtcatcc
     3181 agattcaatg tgtaag
//
LOCUS       DAOQTR010000234         3191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35016_length_3191_cov_3.701849, whole genome shotgun sequence.
ACCESSION   DAOQTR010000234 DAOQTR010000000
VERSION     DAOQTR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35016_length_3191_cov_3.701849"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..1270)
                     /locus_tag="PKN85_06500"
     CDS             complement(404..1270)
                     /locus_tag="PKN85_06500"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNT44093.1"
                     /translation="MKIGIIGAGKVGISLGYVLRGNGADVTVISDRLPSALETARGFL
                     GTDMAYTGDVMEVVRSCSVVAIATQDREIAGIARDIFKAAGDLTGKLFFHTSGADPSS
                     ILSPLDEKGAHLGSLHPLQTFPDIESAIEVLPDTSIFIEGDEKALAVLRVIAGHLGAS
                     IHTIAGKDKVFYHLAAVFVCNLLSALMHSGTGVMDRIDVSLEPFLPIIRATMKNIEAK
                     GPLAALTGPVVRGDDKTIRSHLAAMEDMPLHRDIYLALSKMALEMVRERKALSEAEIE
                     ILRRVLEGGEKT"
     gene            complement(1267..>3191)
                     /locus_tag="PKN85_06505"
     CDS             complement(1267..>3191)
                     /locus_tag="PKN85_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNT44094.1"
                     /translation="GTMEFGKKPRIQLTGKYVIDLQHVGEFVDMRGVVVQKGTAEGTV
                     EVDSGQGKHPLKLGGSGRIKGAEISWKGQSFTVDGPFRLAGREMTFDPIVVTGRETQV
                     TMQGKWGPQGLTTSLKGYIDPGLPGRFAGKTVRASGKAVVDARVTVADGQIGVNGNAN
                     MDDVIFAVPGFIRKTKGVQSRAAVKFTRKKTGEIVVDDLSCNLDTINVRASGKISGDG
                     GIESRVTLRAKDTGRAASLFSLGEDIRGGDVSIDLAVSDLRFPLTKLPWVVGSASMKK
                     GFMKVPGIPKVLANIDLRAGFRGHEFDVTVSGLKTGGSVLKKASLKVTGFEQPKFDVL
                     VSMDKLDMADFGGGKEFKLKSLSKDGILARSSGNLSVRAKEMSFGSIPARDLEINAFM
                     TDRKINVSDLKLRVFGGEMDMKGMLDLSGRVPSLYTNGRMTRVKAGLFMAAMGGTSQE
                     ISGEAFITGTLKTEGTTMKDFKANLGGDTAVYSRDGVIKRWKLLSKIFALLNVYDLVR
                     GKIDFGKDGLAYTKAGASFTINKGIYHTNNFLLDSPSMVITGAGDIDISKETINATLE
                     VSPLVALDRTIDKIPIVRSILKNKNKGFLYVTYSVSGSFDDPDISTNYVGTVGTKSLE
                     ILRNILVFPKEVFERK"
BASE COUNT          590 a         1004 c          873 g          724 t
ORIGIN      
        1 ccagaccttc tcgcctttgg gattcaccgg gaatatcctc ccccggtaat tgccgacgag
       61 gcttttcatc acgtgataac cgagcttgtc gaaactgttg gatgccccga cgaccgcaac
      121 ggagccggga gaaaaaagcc gggcaagctt ctccttcatc acaaaccccc cgtgtcaaca
      181 cattgaatga attccctttt actctgtagc acgacaccaa agaaaaaaca acccacgaag
      241 acctgtcgtg tcatctcccg ccgggacggc gggacgagat cgccgccgga tcctgggggc
      301 tccgagatcc ggcggcgatc ttttctctct tttctcctct ctttgcgacc tctgggagct
      361 tgagagagga gcgaacgcga cgaacgggcg tgagataaag tagttaagtc ttttccccgc
      421 cttcaagcac gcgccggagg atttcgattt cggcttcgct caaggccttc ctctccctca
      481 ccatctcgag ggccatcttc gagagcgcca gatagatatc cctgtggagc ggcatgtcct
      541 ccatggcggc gaggtgggag cggatggtct tgtcgtctcc acgcacgacg ggacccgtga
      601 gtgcagcgag ggggcccttc gcctctatgt tcttcatcgt tgcccgtatg atggggagga
      661 agggttcgag ggacacgtct atccggtcca tcacacctgt tccggagtgc atgagggccg
      721 aaaggagatt gcagacgaag acggccgcga gatggtagaa gaccttgtcc tttcccgcga
      781 tggtgtgaat actggccccc aggtgcccgg cgatgacccg gagcacggcg agggccttct
      841 cgtcgccttc gatgaatatg ctcgtgtcgg gaaggacctc aatggcgctt tcgatatcgg
      901 gaaaggtctg cagggggtgg agcgatccca ggtgggcacc tttttcatcg agaggagaga
      961 ggatcgacga gggatcggcg ccgctcgtat gaaagaagag ctttcccgtc aggtcgccgg
     1021 cggctttgaa tatgtccctc gcaatgccgg ctatctccct gtcctgcgtg gcgatggcca
     1081 cgacgctgca actcctcaca acctccatca cgtcccccgt gtaggccatg tcggtcccca
     1141 ggaaaccacg cgccgtctcc agagccgagg gaagcctgtc ggagatgact gtaacgtcgg
     1201 ccccgttgcc cctgagaacg taacccaggg agatgcccac ttttcccgct cctatgattc
     1261 cgatcttcat tttctctcaa agacctcttt cggaaaaacg agtatgttcc tcaggatctc
     1321 cagcgatttc gtgcccactg ttcccacgta gttcgtgctg atatcgggat cgtcaaatga
     1381 ccctgagacg ctgtatgtga cgtagagaaa acccttgttc ttgttcttga ggatgctcct
     1441 cacgatgggg atcttgtcga tcgtcctgtc gagggcaacg agcggggaga cctcaagcgt
     1501 tgcattgatc gtttccttgc tgatgtcgat gtcgcccgcc cccgttatga ccatcgaggg
     1561 gctgtcgagg agaaagttgt tggtatggta gatccccttg ttgatcgtga aggatgcacc
     1621 cgccttggtg tacgccagtc cgtccttgcc aaaatcgatc ttgccgcgca caaggtcgta
     1681 cacgttgaga agcgcgaata tcttggagag gagcttccat ctcttgatga ccccgtccct
     1741 gctgtagacg gccgtgtcgc cgcctagatt ggccttgaag tccttcatcg tcgttccctc
     1801 cgtcttgagg gtgcccgtga tgaaggcctc gccggatatc tcctgggatg tgccgcccat
     1861 cgccgccatg aagagccccg ccttgacccg cgtcatcctc ccgttcgtat acagggatgg
     1921 gacccgccct gagaggtcca gcatgccttt catgtccatc tcgccgccga acacgcgcag
     1981 cttcaggtca gatacgttta tcttgcggtc cgtcatgaac gcgtttatct cgaggtccct
     2041 ggccgggatg cttccgaagc tcatctcttt cgctctgacc gacaggttcc cgcttgaacg
     2101 cgcgaggatg ccgtccttgc tgaggctctt gagtttgaac tctttgccgc ccccgaaatc
     2161 cgccatgtca agtttgtcca tgctgaccag gacgtcgaac ttcggctgct cgaaacccgt
     2221 gaccttgaga gacgccttct tcaggacgct cccccccgtc ttcaatccgc tcaccgtgac
     2281 atcgaactcg tggccgcgga aacctgccct gaggtcgatg ttggcaagga ccttcggaat
     2341 gccgggcacc ttcatgaagc cttttttcat cgacgcggaa ccgacgaccc agggaagttt
     2401 cgtcaggggg aatcgaaggt cgctgacggc aagatcgatg gatacgtctc cgccccttat
     2461 gtcctcgccg agagagaaga gcgatgccgc cctccccgta tccttcgcgc gcagggtgac
     2521 ccggctctcg atcccgccgt cgccgcttat ctttcccgag gcacggacgt tgatcgtgtc
     2581 gaggttacac gagaggtcat ccacaactat ctcgcccgtc tttttcctcg tgaatttcac
     2641 cgcggccctg ctctgaacgc ccttcgtctt tcgtatgaag cccggcacgg cgaagatcac
     2701 atcatccata ttggcgttcc cgttcactcc tatctgcccg tcggctaccg tcacacgggc
     2761 atcgacgacg gccttccccg acgcccggac ggttttgccc gcaaacctgc ccggaagccc
     2821 cgggtcgatg tagcctttca gggaggttgt gagaccctgg ggtccccatt tgccctgcat
     2881 ggtcacctgc gtctcccggc cggtcaccac aatgggatcg aaggtcattt cccggccggc
     2941 aagcctgaag ggaccgtcca ccgtaaaaga ttgtcccttc caggagattt ccgcgccttt
     3001 tatccggccc gagcccccga gcttcagcgg gtgctttccc tgtccgctgt ccacctcgac
     3061 ggtgccctcc gcggtgcctt tctggacgac cacacccctc atgtccacaa actcgccgac
     3121 gtgctgcaga tcaatgacgt actttcccgt cagctgtata cggggcttct tcccgaactc
     3181 catggtcccc t
//
LOCUS       DAOQTR010000235         3191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35017_length_3191_cov_3.683992, whole genome shotgun sequence.
ACCESSION   DAOQTR010000235 DAOQTR010000000
VERSION     DAOQTR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35017_length_3191_cov_3.683992"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1653)
                     /locus_tag="PKN85_06510"
     CDS             complement(58..1653)
                     /locus_tag="PKN85_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNT44095.1"
                     /translation="MPQENSENDIKTGSPEPPGEELLVCERALGTSERKLSSMLELGR
                     LIGLDLNLDDMLTQIASKAKEVLEADRFNLLLYDPKTDELWTKMVFEIEGREFRTPSH
                     VGLAGYALHTGQTVNVEDAHEDPRFFRYIEEAIGYTTKSMLCMPFFSRSGAALGVMQL
                     INKREGRFTAEDEALLRMFTNHASVFIEIAQLQKERMDTLEESRRELERLNKAKGKAL
                     DHLSHELKTPLALIQGYLRILKRKISKQPQGASVLGYFDTLKHYMTRLFEVQRESEKI
                     ILAYREVEEENLVDELENLWKKIEGMDIPIDTREHWQTLKECVGNYVPRDSGSESVFL
                     LYPVAQKAITDARANAPHRDIEFRLSGDEQASIVMDVVVVRDMMTGLLKNAVENTPDG
                     GLVEVSVKEDERNVTVAVRDYGIGITEQNKEHVFEGFFHTQDTDLYGSKKAYDFGAGG
                     KGLDLFQMKVYGRRFGFDISMTSSRCDHIPTDRDLCPGRISECRFVSRREACLEAGGT
                     TFYLTFPAAKEYLRTSTRDESDP"
     gene            complement(1722..2357)
                     /locus_tag="PKN85_06515"
     CDS             complement(1722..2357)
                     /locus_tag="PKN85_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNT44096.1"
                     /translation="MTRSFAGTAGLYCVPLICLALIAGCLGRTKPPYVMDQYTLDYAP
                     GAVTAGQPVQELIRVERFAVAPAFNSTNMAIRKGQYRFETYNYSRWRVNPADMVAGFV
                     LRDITRAGIFKGTYSWYDSDLSRYILEGYVDEFCESSGKGLLSVRITLVDTKAKNPVE
                     QVVFQRQYTQSAPMDDSSPDALAAALSAAMKEVSGRLMTDIAATLRESADK"
     gene            complement(2354..>3191)
                     /locus_tag="PKN85_06520"
     CDS             complement(2354..>3191)
                     /locus_tag="PKN85_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT44097.1"
                     /translation="KYFEQGTVHVTYFDESVQGLQVDSSVKYRGVEIGMVEKIGVAPD
                     YRLIEVVMKINFSGDAANATTAKLKAAGITGIVYVELDHRKKGDLAKTPQITFEPDYP
                     VIPSNPSEIQEIVSGVDDVVKKMKEVDFKAISDELVSATKSVNTFFAGQKMTRIITNL
                     ENMTQTLSDSANKLAKMVGDGRADQMLSDARDTVRDAKETIARVRSELEKMKLAETAA
                     KTTRMIEDLSRRSKTITMEVQLTGENLRRASEDLEQLIERLKADPSDILFSDPPPKRG
                     KR"
BASE COUNT          564 a          924 c          924 g          779 t
ORIGIN      
        1 atcatggata agcaggtcaa cgagggggtc tttacgggtc atttaccagg ccttggatta
       61 aggatctgac tcgtccctag tactggttct aagatattct ttcgcggcgg gaaaagtcaa
      121 ataaaaagta gttccgccgg cttccaggca ggcctcccgg cgcgagacga aacggcattc
      181 ggagatcctt ccgggacaca ggtccctgtc ggtggggatg tgatcgcagc gggaactcgt
      241 catcgagatg tcgaagccga aacgccgccc atacaccttc atctggaaaa ggtcgagtcc
      301 cttaccgccg gctccgaagt cataagcctt cttcgaaccg taaaggtcgg tgtcctgggt
      361 gtggaagaaa ccctcgaaga cgtgttcctt gttctgttca gtgatcccga tcccgtagtc
      421 cctgacggcg acggtgacgt tcctctcgtc ctccttgacg gacacctcga cgaggccccc
      481 gtcaggcgtg ttctccacgg cattcttgag aagccccgtc atcatgtccc gcaccacgac
      541 gacgtccatg actatggagg cctgttcgtc tccgctcaag cggaattcga tatcgcgatg
      601 gggtgcgttg gcccgggcgt cggttatggc cttttgggcc acgggataga ggaggaacac
      661 gctttccgat ccgctgtcgc gcggcacgta attgccgacg cattccttga gggtttgcca
      721 gtgttccctg gtatcgatgg ggatgtccat cccctcgatc ttcttccaga ggttctccag
      781 ttcgtcgacg aggttctcct cctccacctc gcgataggcg aggatgatct tctcactttc
      841 cctctgcacc tcgaaaagcc gcgtcatgta atgcttgagg gtgtcgaaat accccagcac
      901 cgacgcgccc tgtggctgtt tcgatatctt cctcttgaga atgcgcagat acccctggat
      961 gagcgcaagg ggtgtcttca gttcatggga gaggtggtcg agggccttgc cttttgcctt
     1021 gttgaggcgt tccagttccc tgcgggactc ctccagggtg tccatgcgtt ccttctggag
     1081 ctgggcgatc tcgatgaaga ccgatgcgtg gttggtgaac atcctgagga gtgcctcgtc
     1141 ctctgcggta aaccttccct cacgcttgtt gatgagctgc atcaccccga gagctgcgcc
     1201 ggacctcgag aagaaaggca tgcagagcat gctcttcgtc gtgtagccga tggcctcttc
     1261 gatgtagcgg aagaaacgcg ggtcctcgtg ggcgtcctcg acattgacgg tctggcctgt
     1321 atggagggcg taccccgcga gtccgacgtg ggatggcgtg cggaactccc tgccttcgat
     1381 ctcgaagacc atcttggtcc agagctcatc cgttttcggg tcgtaaagga gcaggttgaa
     1441 cctgtccgct tcgaggacct ccttggcctt cgaggctatc tgggtaagca tgtcgtcgag
     1501 gttgagatcg aggccgatga ggcgtccgag ttccagcatg gacgagagct ttcgctccga
     1561 tgtacccaag gcccgttcgc agacaaggag ctcttctccg ggcggctccg gggaccctgt
     1621 tttgatgtcg ttttccgagt tttcttgagg cacgtgcccg atccttacgc ttgcgaccta
     1681 agtaatattt gtcaaaaagg gcggtctgtc aagacttcct gtcatttatc ggcgctttcc
     1741 ctgagagtgg cggctatgtc ggtcatgagc ctgcccgaga cctccttcat ggccgcgctc
     1801 agtgccgccg ccagggcgtc gggagaactg tcgtccatgg gagccgactg ggtgtactgc
     1861 ctctgaaaca ccacctgctc cacggggttc ttcgccttcg tgtccacaag ggtgatgcgg
     1921 acgctcaaga gccccttgcc cgaggattca cagaactcgt cgacatagcc ctccaaaata
     1981 taacgggaga ggtcggaatc ataccaggag tatgttccct tgaatatccc ggcacgggtg
     2041 atgtctctga gcacgaagcc cgcgaccatg tccgccgggt tgacgcgcca gcggctgtaa
     2101 ttgtatgttt cgaaacggta ctgtcctttc ctgatcgcca tgttggtgga gttgaatgcc
     2161 ggcgccacgg caaacctttc cacgcggatg agctcctgga cgggctgccc ggcggtgacg
     2221 gccccgggag cgtaatcgag cgtgtactgg tccatgacgt agggtggttt cgtcctgccg
     2281 agacaccccg caatgagtgc aaggcatatc aggggcacac aatagagccc cgctgttccc
     2341 gcaaatgatc ttgtcatcgt ttacctcttt tcgggggcgg atcgctgaac aggatgtccg
     2401 acgggtccgc tttgagccgt tcgatgagct gctcaaggtc ctcggatgcc ctgcgcaggt
     2461 tctcgccggt gagttggacc tccatggtga tcgtctttga cctgcgcgag aggtcctcga
     2521 tcatcctggt ggtcttagct gccgtttcgg caagcttcat cttctcgagc tccgacctca
     2581 cccgggctat cgtctccttc gcgtcgcgca ccgtgtctcg cgcgtcgctc agcatctggt
     2641 cggcccttcc gtcgcccacc attttcgcca gcttgttcgc ggaatcggaa agggtctggg
     2701 tcatgttctc caggttcgtg atgatcctcg tcatcttctg tccggcgaag aaagtgttga
     2761 cgcttttggt tgccgacacg agttcgtcgg atatggcctt gaagtccacc tctttcattt
     2821 tcttcaccac gtcgtcgacg ccggacacga tctcctgtat ctccgacgga ttggagggga
     2881 tgacgggata gtcgggttca aaggtgatct gcggtgtttt ggcgaggtcc ccttttttgc
     2941 ggtggtccag ctcgacatag actatccctg ttattcccgc cgccttcagc ttggctgtcg
     3001 ttgcgttggc cgcatcgcca ctgaaattga tcttcatgac gacctcgatg aggcgataat
     3061 ctggcgcgac gccgatcttt tccaccatgc cgatctcgac gcccctgtac ttgacgctcg
     3121 agtcgacctg aagcccctga acggattcgt cgaaataggt gacgtggacc gtcccctgct
     3181 cgaagtattt g
//
LOCUS       DAOQTR010000236         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35109_length_3186_cov_3.505270, whole genome shotgun sequence.
ACCESSION   DAOQTR010000236 DAOQTR010000000
VERSION     DAOQTR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35109_length_3186_cov_3.505270"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKN85_06525"
     CDS             <1..314
                     /locus_tag="PKN85_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44098.1"
                     /translation="VLEPLLFDEHIELFRSLASLKQRLCLPAEGRVMAIIFVSDREDL
                     MDILSMRYFLCNVEIVLILPSRKKDIVALGHELRPRFMTYKDADFGEMLSVIKKMTGG
                     W"
     gene            516..767
                     /locus_tag="PKN85_06530"
     CDS             516..767
                     /locus_tag="PKN85_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44099.1"
                     /translation="MRERCMIGTVAVLGLSLAVSGMWLAQSSRCVILEKEGSRVLVSC
                     NGKPARYIDLGGRADMYRVGDSIDDRTFPGASDRKVKEK"
     gene            782..1567
                     /locus_tag="PKN85_06535"
     CDS             782..1567
                     /locus_tag="PKN85_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNT44100.1"
                     /translation="MKPRMPLSALVLAAAVLLCACAPNSVINPASISQMDQQSRAYPQ
                     KEYVIAAGDVLDIKFVGGAEYNETGVTVRPDGRISLALAPEMRVAGLTPGEMANQLRQ
                     KYASELKRPEVVITVRGFSDQKVFVDGEVLLPGVVELKGPTTVMRAIAQARGLRETAR
                     LSNVIIIRKDFEGKPMAANLDLGKVINGTDLSQDIYLMPYDIVYVPKSNIARLNKFVD
                     EYINRVVPGGFPGFQQFTNPYRNSLGGFTNVYPDSGASVTVAP"
     gene            1591..2973
                     /locus_tag="PKN85_06540"
     CDS             1591..2973
                     /locus_tag="PKN85_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNT44101.1"
                     /translation="MTEKRHQPIDFTNIRDLLSVIFKRKYTILAVFVVVFGTVALYAF
                     LAPRIYEAKSILLVKLGREFMRTSEGANSSPGLSVQPETIMKSEISILTSKDLLTRVI
                     NTLGIGRIYPAIAKRSATQTDAEQAAIASFEENLRVTNIPGSGLIQVAFSHGNPSTSA
                     TVVNTLVDAFKDKHLDVFGGKTTAFLERQEKAFQERLKESENNLSAFKLKNKVFSFEE
                     QKTNLIDLLGTLDEKLKTAQNEVTELEQRAAFVRGSRWTADVPVETRNQLVALQQKEQ
                     GLLERYTENSRMVGTVRQELNAMKDAIGKATEQARSVEVARIEGQLAGARARAGSIRA
                     QMRQVEGELNALDGHGRALQGLKREAAQLEQNHQIYSRKLEESLIMDDMDRQKMVAVS
                     VVQKATVPAFPKKQKLNRSQMVGAGFFGGLAAGIALAIILELMTPGMPTPASAEKSLG
                     VPVLITVTKK"
BASE COUNT          745 a          884 c          978 g          579 t
ORIGIN      
        1 gcgtgctgga gcctcttctc ttcgatgaac acatagagct cttcaggagt ctcgcgagtc
       61 tgaagcagcg gctctgcctt cccgccgaag gcagggtgat ggccatcata ttcgtttccg
      121 acagggagga cctcatggac attctctcga tgagatattt cctgtgcaat gtcgaaatag
      181 tgctgatact gccgagcagg aagaaggata tcgtggcact ggggcacgag ttgaggcccc
      241 ggttcatgac gtacaaggat gccgatttcg gcgaaatgct gtcggtcatc aagaagatga
      301 ccggcggctg gtgaaggaat agcggggcgg cgcggcgtga gggatgagcc ctcgcggcac
      361 cgcccttgaa cagcgaatgg atgcaggaaa ggaacatgtc cccgtcgcgg gacgacgatg
      421 cacgagacag ccgggaggtt gaatggcggt agcctgcccc gaaacgatcc ttttcctgcc
      481 ttcaaggggg cagcaacttc tcccaggagg tgaagatgag agagcgatgc atgataggga
      541 cagtggccgt tctcgggttg tcccttgcgg tctccggtat gtggctcgcc cagtcttccc
      601 gttgcgtcat ccttgaaaag gagggctcga gggtgctggt gagctgcaac ggcaaacctg
      661 cgcgatacat cgatctcggc ggcagggccg acatgtaccg cgtgggcgac agcatcgacg
      721 acaggacgtt tccgggagcg agcgaccgga aagtgaagga aaaatgaagg aggtggagat
      781 aatgaaacct cggatgccat tatcagctct tgttctcgcg gcggccgtgc tcctttgcgc
      841 gtgcgcgccc aattccgtca tcaatcccgc atccatcagc cagatggacc agcagtcgcg
      901 ggcttatccc cagaaggaat atgtgatcgc tgcgggagat gttctggaca tcaagttcgt
      961 gggcggtgcc gagtacaacg aaacgggggt cacggtcaga cccgacggac ggatatccct
     1021 cgccctggcc ccggagatga gagtcgcagg cctgacgccg ggtgaaatgg cgaaccagct
     1081 gagacagaag tacgcgtcgg aactgaaaag gccggaggtc gtcatcacgg tgcgtggttt
     1141 ttccgaccag aaggtctttg ttgacggcga ggtccttctc cccggcgtgg tcgagctcaa
     1201 gggtccgaca acggtcatga gggcgatagc ccaggcgcgg ggactccgcg agacggcacg
     1261 gctctccaac gtgatcatta tccgcaagga ctttgaaggc aagcccatgg ccgccaacct
     1321 ggacctggga aaggtcatca acgggacgga cctgagccag gatatctacc tcatgcctta
     1381 cgacatcgtc tacgttccca agtcgaacat agcgaggctc aacaagttcg tggacgaata
     1441 catcaacagg gtagtcccgg gcggtttccc cgggttccag caattcacca acccctacag
     1501 gaattcactc ggcggtttta ccaatgtcta tcccgactca ggcgcctctg tgaccgtggc
     1561 gccgtgacaa caaagggagg ttgacgaatc atgaccgaaa agcggcatca acccattgac
     1621 ttcaccaaca tacgggacct tctctccgtt atcttcaaac gcaaatacac gatcctcgcc
     1681 gtatttgtcg ttgtcttcgg aacggttgcc ctgtatgcgt ttctcgctcc ccggatctac
     1741 gaggcgaaga gcattcttct cgtcaagctg ggaagagagt tcatgaggac atcggagggg
     1801 gcgaactcga gccccgggct ttccgtccag cccgagacga tcatgaagag cgagataagc
     1861 attctgacaa gcaaggacct cttgacacgg gtcatcaaca cgctggggat cggacggata
     1921 tacccggcga tcgcgaagag gtccgccacg cagaccgacg cggagcaggc ggccatcgcc
     1981 tctttcgaag agaatctcag ggtgacgaac attcccggtt cgggcctcat ccaggttgcc
     2041 ttcagccacg gaaacccttc cacatccgcg acagtcgtca acaccctcgt ggacgctttc
     2101 aaggacaaac acctcgatgt cttcgggggc aagaccaccg ccttcctcga gcgtcaggag
     2161 aaggcctttc aggagaggct gaaggaatcg gagaacaacc tgtccgcctt caaactgaag
     2221 aacaaggtct tttccttcga ggagcagaag acgaacctga tagatctcct cgggaccctc
     2281 gatgaaaagc tgaaaacggc ccagaatgaa gtgaccgagc ttgaacagag ggcggccttc
     2341 gtgcgcggtt ccaggtggac cgccgatgtt cccgtggaga cgcgcaatca gctcgttgcc
     2401 ttgcagcaga aggaacaggg gctcctcgag aggtacacgg agaactccag gatggttggg
     2461 accgttcgcc aggagctcaa tgcgatgaag gatgccatcg gcaaggccac cgagcaggca
     2521 aggagtgtcg aagtggccag gatcgagggg caactggcgg gcgcgagggc gagggcaggc
     2581 agcatacggg cgcagatgag gcaggtcgag ggcgaactca acgcccttga cgggcacggc
     2641 agggcattgc agggcctcaa gcgcgaggcc gcccagctgg aacagaacca ccagatctat
     2701 tcccggaaac tggaggaatc cctcatcatg gacgacatgg atcggcagaa gatggtggct
     2761 gtcagcgttg tccagaaagc gacggtcccc gccttcccga aaaaacagaa actcaacaga
     2821 agccagatgg taggcgcggg cttcttcggc ggcctcgcgg cgggtatagc tcttgccatc
     2881 atactcgagc ttatgacgcc cggcatgccg acgcccgcca gcgcggagaa gagcctgggc
     2941 gtgcccgtcc tcatcacggt gacaaagaaa tgacagcacg ccggctgacc aaaaaaccta
     3001 tgaggggatg gtgaaacatg tatctcaact attacaatct ccggaagcag cccttccaca
     3061 taacgccgga ccccgagttc ctctatctca gcccgagtca caaggaggcg cttgccgcca
     3121 tcatttacgg catagaggag aagaaggggt ttgtggccat tgtgggagct gtcggtgtcg
     3181 gaaaaa
//
LOCUS       DAOQTR010000237         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35202_length_3181_cov_4.050544, whole genome shotgun sequence.
ACCESSION   DAOQTR010000237 DAOQTR010000000
VERSION     DAOQTR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35202_length_3181_cov_4.050544"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="PKN85_06545"
     CDS             complement(<1..1749)
                     /locus_tag="PKN85_06545"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNT44102.1"
                     /translation="MTRLRKILLWLLIAVALFTVTGFFVAPPVLKSVLIKKLSASLGR
                     TVSIGKISVNPYTLGVRFRNITVSERSGPGTFFRVDEIRARIGLSAVTGVITLGHLSL
                     RNPHVNITRYDDGSYNFSDLLEKKGTPAGETTGEKDGITFSLRDIAIQNGSADLLDTP
                     VKKKHTLREFNLSVPLLSNAPRHVERDVRPTLSFKLNDDPYVITGRTRPFADSLETFF
                     DINIENVDIPRYLAYLPVKPRFSVPSGYLDIRIEAAFRQFKDKVPLLALKGDMTVSKL
                     VVNDEKGKTVVSLPALRVAVASIEPLAGKMELSKVSIESPEFTLDRDRNGALNIVALV
                     PEMKEKQKSNGNTGKTARAGKKADGGPFQFIVDSVELTGGKVLFTDHSLKDPAKVTVE
                     KLDLKGQKISLTEGSPGAFSTSLVVNRKGAVKLEGTVALDPFSLKAKVDLKGIDIRPF
                     QPYFTDRVRVSVMSGAAGASGDLVVTQAPKKGPSVHYTGSMSVSGLSSVDKETAESLL
                     KLKSFHIRNVEFGYNPTRVTAKGVSLTDFYANIAVRPDGTLNLQQTIVKDDAEADGRI
                     GTTDNKTTAVEKGRAAP"
     gene            1915..>3181
                     /locus_tag="PKN85_06550"
     CDS             1915..>3181
                     /locus_tag="PKN85_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNT44103.1"
                     /translation="MAEKGRSTKTLNVQAVPAVVSCYTGVVKVLLVQPPIEDFYDTSI
                     RTYPLGLSYIAARVRDICDVAILDCRTGFGPRHPEDEGFPELAPYYRDDRQTPFSLFR
                     RYARYGMAGREIEAAIREARPDVVAIASSITAYSREARKVAAIAKGVDDGIITVMGGT
                     HATLFPGQLLGGGNVDYVVRGEGETPFFELTVALASGNAGSMTSIPGLSFHRCGSLHI
                     GGTHIEDDIDLIPARDLLPVERYRIGKRPYTFLLTSRGCPFGCSFCGKPPVPYRRRAL
                     QSIEKELSGIERLGITSIDFEDDMLNLDIPFFHRVLDLVKGRGFVLSAMNGLYSHTLD
                     TATLTKMRDAGFSRLNFSLVDTSPTVHSTQRRRYPANFLGLLDWLESSDFLVETHFII
                     GLPGQTPREIIDTMTFLAGKRMLPGPSVYY"
BASE COUNT          620 a          925 c          900 g          736 t
ORIGIN      
        1 aggagccgca cgccccttct ccacggccgt cgttttgttg tcggtcgttc cgatgcggcc
       61 gtccgcctcc gcatcatcct tgacgatcgt ctgctggagg ttcagcgtcc cgtcgggtct
      121 gacagcaata ttggcgtaaa agtcggtgag cgatactcct tttgcggtaa cccgggtagg
      181 gttgtatccg aattcgacgt ttcggatgtg gaaagacttc agcttgagca gactctccgc
      241 cgtctccttg tcgacggatg agaggcccga gacggacatg cttcctgtgt aatgcacgga
      301 aggccccttc tttggcgcct gtgttactac gaggtctcca ctcgcgcccg ccgcaccgct
      361 catcaccgat accctgaccc tgtcggtgaa atagggttga aaggggcgga tatcaatgcc
      421 cttgaggtcc accttcgcct ttaaggagaa cgggtccaat gccaccgtcc cttccagttt
      481 cacggcccct ttcctgttga cgacgagaga tgtggagaac gcaccgggag agccctcggt
      541 gagcgagatc ttctgaccct tcaggtccag cttctcgacg gttaccttcg ccggatcctt
      601 gagagaatgg tcggtaaaga gaacctttcc tcccgtgagc tcgacactat cgacgatgaa
      661 ctgaaaagga ccgccgtcgg ctttcttccc ggccctagct gtctttcccg tgttgccgtt
      721 gcttttctgc ttctccttca tttccggtac aagggccacg atattcagcg cgccgttccg
      781 gtcacgatcg agggtgaact caggggattc gatggagacc ttcgagagtt ccatctttcc
      841 ggcaaggggc tcgatcgacg cgacggcgac ccttaaggcc ggaagactca cgaccgtttt
      901 gcccttctca tcgttgacga cgagctttga caccgtcata tcacccttga gggcgagaag
      961 aggaacttta tccttgaatt gcctgaaggc tgcctctatt ctgatatcga gatagcctga
     1021 ggggacggag aaccggggtt tcaccgggag ataggcaagg tagcggggaa tgtccacgtt
     1081 ctcgatattg atgtcgaaga acgtttccag ggagtcggcg aagggcctcg tccttcccgt
     1141 gataacgtaa gggtcgtcat tcagcttgaa tgaaagtgtg ggacggacat ctctttcaac
     1201 gtgcctgggg gcgttggaca agagggggac gctgagattg aattccctca gggtgtgttt
     1261 cttctttacc ggcgtgtcaa ggagatcggc gctgccgttc tggatcgcta tatcgcgaag
     1321 cgaaaaggtg atgccatcct tttcccccgt ggtttccccg gcgggagtcc ctttcttctc
     1381 gagaagatcg gagaagttgt atgatccgtc atcgtagcgg gttatgttta cgtgaggatt
     1441 tctgagcgag aggtgaccga gggttatgac gccggtgaca gcggaaagac cgatcctggc
     1501 ccgtatctcg tcgacacgga agaacgtgcc gggaccggag cgctcgctaa cggtaatgtt
     1561 ccggaaacga acacccagcg tgtaagggtt gacgctgatc ttgccgatgg ataccgtcct
     1621 accaagactg gcggaaagct tcttgatgag gaccgatttc aggaccggcg gagcgacaaa
     1681 gaaacccgtg acagtaaaaa gggcgacggc gatgaggagc cataaaagga tctttcttag
     1741 ccttgtcatc cgacattctc ctttcccctt ccttgcaaat acataccatc ccgcgggtgg
     1801 gatcgctacc tctttttccc actactttcc gggtcacgtg cgcgtcttcc acgctcgacg
     1861 cgcaggacgc gggaaactcg tccgtcaccg ttcccgcgtc tgaagtcata tacaatggct
     1921 gaaaaaggaa gatccaccaa gactctgaac gttcaagcgg ttccagccgt tgtttcgtgc
     1981 tatactggcg tcgtgaaggt ccttctcgtc cagccgccca tcgaggactt ctacgatacg
     2041 tccatcagga cctatccgct gggactttct tacattgccg caagggtgcg ggacatatgc
     2101 gatgtggcca tccttgattg caggacgggt tttggcccgc ggcaccccga agatgaggga
     2161 ttcccggagc ttgcgccgta ctaccgcgat gacaggcaga cgcctttttc gttgttcaga
     2221 cggtacgccc gttacgggat ggccggtcgt gagatagaag ccgccatccg ggaggctcgt
     2281 cccgatgtcg tggcgatagc atcttctatc accgcctatt cgcgagaggc ccggaaggtc
     2341 gccgccatcg cgaagggagt ggatgatggg attatcaccg tgatgggcgg gacccacgcg
     2401 accctcttcc ccgggcaact cctcggtgga ggtaatgtcg attacgtcgt tcgcggcgaa
     2461 ggggagacgc cttttttcga attgaccgtc gcccttgctt cggggaatgc cggcagtatg
     2521 acctccatcc cgggactctc ctttcaccgc tgcgggtccc ttcatatcgg gggcactcat
     2581 atcgaggatg acatcgacct cattccggcg cgcgatcttc ttcccgtcga gaggtatcgc
     2641 attggcaaga ggccgtacac ctttctcctc acgtcgagag ggtgcccctt cgggtgttcc
     2701 ttctgcggca agcctcccgt tccctaccgc cgccgggccc ttcagtccat cgagaaggaa
     2761 ctttccggca ttgagaggct ggggataacc tccatagatt tcgaggacga catgctcaac
     2821 ctcgacatcc ccttcttcca ccgtgtcctt gacctcgtga aggggagagg gttcgtcctt
     2881 tccgccatga acggtctcta cagccacacc ctcgatacgg ccacactgac gaagatgcgc
     2941 gacgccggtt tctcgcgtct caacttctcc ctcgtcgata cctcgccgac agtccacagc
     3001 acgcagcgaa ggaggtatcc ggcgaacttc ctcggccttc tcgactggct ggagtcttcg
     3061 gactttctcg tggaaaccca tttcatcatc ggccttccgg gccagacacc gcgagagatc
     3121 atagacacga tgacctttct cgcgggaaag aggatgctgc ccggcccgag cgtctattac
     3181 c
//
LOCUS       DAOQTR010000238         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35249_length_3178_cov_3.775857, whole genome shotgun sequence.
ACCESSION   DAOQTR010000238 DAOQTR010000000
VERSION     DAOQTR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35249_length_3178_cov_3.775857"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="PKN85_06555"
     CDS             complement(<1..1864)
                     /locus_tag="PKN85_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNT44104.1"
                     /translation="MFVASVLAFVAGIYSEASRQIPFFPLLISLAVSLVLVPFLITKG
                     FLRAAGTALLVAFLLAGMLRLALVFLDQPPPAIDPGPSLYRGTVAESSKDVRIIEIEE
                     PPHLSGIRALIRSDHPSAIGDRVTVLGTLRDIVPTFKNPHHVSWRWVKKLEGTFCEIR
                     GEIVSARPGKKLIDGWRRYLAGKVDASGTRHAPVVKALTIGDTASLDDRTKTLFLETG
                     TSHILSISGSHFAVVAGFFFFIARLAFRASSRMRQKGTDRAWAALLTIPFTILFMLVS
                     GSSLPTIRATIMIIVYMLALFFERKGHSVNALFLSALIILVIFPHSLFSPSFQLTFVS
                     VLFIILTSRAVHSLLAKTGRAVRWLLSLTAVGLAATIGTLPVVLYHFHGFNPLGFLYN
                     LAAVPLMCLVSTPLALAGLVAPFGAYLLRLSGEVIEITIALLDTLRRGYIYPVIRPNL
                     PEALIYFATLLSLLFIRRRPVRFAFVAIVLPLLIVTGFVTWQKRFHNNDLCVSYIDVG
                     LGDAILVEAPRGVRLLIDGGGFHGTGFDTGRSVIAPLLLGKKIATLDYVVNSHPHEDH
                     IGGLRFILGHFGVRAYMGLTGPEAQPSMKRVTDILRERCIPSVHLAAGDIIPLPS"
     gene            complement(1855..2610)
                     /locus_tag="PKN85_06560"
     CDS             complement(1855..2610)
                     /locus_tag="PKN85_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44105.1"
                     /translation="MEKIKTIYSPRFLTDYPTVDCESPDRIEFIHSKIKDIAEFIEPE
                     PCALSDLALCHSESLIKTVARTKDLYDTAILAAGGAMMAAETALREPCFALVRPPGHH
                     AGHDFNGGFCFFNNMALAVKGLLAHDTIESALILDIDLHYGNGTHDIVRSDPRIIFRN
                     IDARSREGFFKDLDEALSDASKYDVLGCSVGFDTYIRDWGGMLYGEDYHRVGSSLVSA
                     NRRLFCVLEGGYYVADLGTNTQFLLTGILKACS"
     gene            2873..>3178
                     /locus_tag="PKN85_06565"
     CDS             2873..>3178
                     /locus_tag="PKN85_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT44106.1"
                     /translation="MKRIIVGTAGHIDHGKTTLIRALTGIDCDRLKEEKERGITTELG
                     FAHWHLDDDLLVGIVDVPGHERFVRHMVAGAWGIDTVLLVVAADEGVMPQTREHLDIC
                     "
BASE COUNT          735 a          812 c         1040 g          591 t
ORIGIN      
        1 ctgaggggag gggaatgata tcgccggctg ccaggtgcac ggagggtatg caccgctctc
       61 tgagaatatc cgtgacgcgc ttcatcgacg gctgcgcttc gggacctgtc aggcccatgt
      121 aggccctcac gccgaaatga ccgagaatga aacgcagccc tccgatgtgg tcctcgtggg
      181 gatgactgtt cacgacgtag tccagggtcg ctatcttctt tccgaggagg agaggagcga
      241 tgacggacct tcccgtatcg aacccggtgc catggaaacc gcctccgtcg atgagcaggc
      301 ggacgccgcg gggagcctcg acgagtatgg catcgcccaa ccccacgtcg atgtagctga
      361 cgcacaggtc gttgttgtgg aaacgcttct gccacgtgac gaaacccgtg acgataagca
      421 gcgggaggac gatggcgaca aaggcgaaac gcaccggtct gcggcggatg aagaggagcg
      481 agaggagggt ggcgaaatag atgagtgctt cgggaaggtt cggacgtatc acgggataga
      541 tatacccgcg cctcagtgtg tccagcaggg caatggtgat ctcgatgacc tctcccgaca
      601 gccgcaggag gtacgcgccg aaaggcgcga cgaggcccgc gagggcgagc ggcgtggaga
      661 caaggcacat gagcggtacg gcggcaaggt tatagagaaa gcccaggggg ttgaatccgt
      721 gaaaatgata gaggacgaca ggcagggtcc cgatcgtggc cgcgaggccc acggccgtca
      781 gagacaggag ccatcggacc gcgcgaccgg tcttcgccag cagggaatgg accgcgcggc
      841 tcgtgagaat aatgaagaga acgctgacga acgtcaactg gaaggacggg gagaacagcg
      901 agtgggggaa tatgacgagt atgatgaggg cgctgaggaa caacgcgttc acgctgtgcc
      961 ccttgcgttc gaaaaagagc gcaagcatat agacgatgat catgatcgtc gcgcgtatgg
     1021 tgggaagact cgaaccggag acaagcatga agagaattgt gaaaggtatg gtcaggaggg
     1081 cggcccatgc gcgatcggtc cctttttgac gcattctgga agatgcgcgg aaggcgagcc
     1141 gcgcgatgaa gaagaaaaag ccggccacga cggcgaagtg tgacccggat atggacagga
     1201 tatgagatgt tcccgtttca aggaagaggg tcttcgtcct gtcgtcaagg cttgccgtat
     1261 cgccgatggt cagggccttc acgaccgggg catgccgggt accgcttgca tcgaccttcc
     1321 cggcgagata tcttctccag ccgtctatca gtttctttcc cgggcgtgcg gacactatct
     1381 ctccacggat ctcgcagaag gtcccttcga gtttcttcac ccatctccac gagacgtgat
     1441 gggggttctt gaaggtgggg acaatgtctc tcagagtgcc tagcacggtc acccggtcac
     1501 caatggcgga agggtggtcc gaacggatga gggcccgtat gcccgagaga tggggaggct
     1561 cctcgatctc gatgatccgt acgtccttcg atgactcggc gacggtgccc cggtacagcg
     1621 acgggcccgg atcgatggcg ggaggaggtt ggtcgagaaa taccagggcg agcctcagca
     1681 ttccggcgag gaggaaagca acgagaagcg ccgtgcccgc cgcccggagg aatcccttcg
     1741 tgatcagaaa gggaacgagg acgagagaaa ccgcgaggct gataagtaaa gggaaaaagg
     1801 gtatttgacg ggaggcctcg gagtatatgc cggcgacaaa ggcaaggacg gaggctacga
     1861 acaagccttg aggatacccg tgagaaggaa ttgcgtattc gttcccagat cggccacgta
     1921 ataccctccc tcgaggacgc agaaaaggcg gcgatttgcc gacacgagag aggaaccgac
     1981 cctgtgataa tcttccccgt acagcatccc gccccagtcg cggatgtagg tgtcaaaccc
     2041 cactgagcac cccagaacat cgtatttgga ggcatcggag agcgcttcat caaggtcttt
     2101 gaagaatccc tcgcgggaac gggcgtcgat attcctgaag atgatgcgcg ggtcggaacg
     2161 gacgatgtcg tgggtgccgt tgccatagtg gaggtcgatg tccaggatga gggcgctttc
     2221 gatcgtatca tgcgcgagga gacccttgac cgcgagagcc atgttgttga agaaacagaa
     2281 accgccgtta aaatcgtgac ccgcgtggtg tcccggcggc ctgacgaggg caaagcaggg
     2341 ttctctgagc gccgtctcgg cggccatcat ggcgccgccc gcggcaagga ttgccgtatc
     2401 gtacagatcc ttcgtgcggg caacggtctt tataaggctt tcactgtggc acagcgcgag
     2461 gtccgacagg gcgcagggct cgggttcgat gaactcggct atatccttga tcttcgaatg
     2521 gatgaattct atccggtcag ggctctcgca gtccacggtg ggatagtcgg tgagaaacct
     2581 ggggctgtat atggtcttta tcttttccat gcctttatta atcacaaaga cgggggcaag
     2641 tcaatgagga aaagacggag gataggggac gggggataga aacggttcaa gggttcaaaa
     2701 gttcaagagt tcaagagtaa aaaggaaaga acgcgggatg ccttcggccg gtgtgcaaat
     2761 tgtgcaaaca gccccccttc gcgacctttt ccgtgaaccg tgaaccgtga accttgaact
     2821 gcctttcccc cctttccctg cggtcccatt cgtggtatag tcttagcctg tcatgaaacg
     2881 tataatcgta ggaaccgcag gtcatatcga tcatggtaag acgaccctga tcagggcgct
     2941 cacgggtatc gattgcgatc gtctcaagga agagaaggaa aggggcatca cgacggaact
     3001 cggttttgcc cactggcatc tggatgacga cctgcttgtc ggtatcgtcg atgtcccggg
     3061 acatgaaaga ttcgtgcgtc acatggttgc gggcgcctgg ggcatcgata cggtgcttct
     3121 cgttgttgcc gccgatgaag gggtcatgcc gcagacccgg gagcatctgg acatatgc
//
LOCUS       DAOQTR010000239         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35271_length_3177_cov_3.673286, whole genome shotgun sequence.
ACCESSION   DAOQTR010000239 DAOQTR010000000
VERSION     DAOQTR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35271_length_3177_cov_3.673286"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            530..1057
                     /locus_tag="PKN85_06570"
     CDS             530..1057
                     /locus_tag="PKN85_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:929..931,aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNT44107.1"
                     /translation="MDNDERLKGWNPYLAGALTGLLLILSVWVAGKYFGASTTFVRST
                     GLVEKVFSQERVAQMEYFIKELPKIDWQWMFVVGIFFGSLIASTTSKSFRWQGVPTMW
                     EARFGPNRAKRAVTAFLGGAIAMFGARLADGUPSGHGLSGSLQLAVSGYISLICFFVG
                     GLIVARILYQGGAKK"
     gene            1054..1581
                     /locus_tag="PKN85_06575"
     CDS             1054..1581
                     /locus_tag="PKN85_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNT44108.1"
                     /translation="MNDLLYGIITGIIFGFLLQKARVIRYDKQLAALRLMDMTIVKYM
                     FTTVLVAMVGVYLLKDLGLVKLSIKTTILGGNIIGGLIFGAGWGLLGYCPGTSLGALG
                     EGRWDSIWGIVGMLAGAAVFAELFPLMKRTVLTWGNLGKITIPGILGINHWIVIVIVV
                     VLAVLMFRFFEKKGL"
     gene            1815..>3177
                     /locus_tag="PKN85_06580"
     CDS             1815..>3177
                     /locus_tag="PKN85_06580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT44109.1"
                     /translation="MSAERLVVIGGVAAGMSAASSFKRLKPEAEAIVLQNDHFISYGA
                     CSLPYYISDDVKEINRLISLSPEVATKERGITVLTRHEARSIDPARKTVTAVDLDGNK
                     EKTISYDHLVVATGGLPVRPPFPGIDLGHIFTLRTLHDGIEIKRFIDRWTTFEPCVGP
                     QCVYVNPFGAEKRPMRASIVGGGSIGMEMCESLRKRRVEVTVFEKMDRVLGTMDTSIT
                     NIVEERLAAEGVNLMKGVSVQSFEGDSGSVTRVITDKGTFDTDMVLLVIGARPNTKIA
                     VDAGIELGAGGAIKVDDHLRTNIPDIYAAGDCAEATQMVTGKKAYIPLGATANKQGRI
                     AGENAAGMNNAFEGVVGTAVTKIFDLEVARTGLSPLEAEREGIDHFVSTIKGRSRSTA
                     CPEGKPITVTDPEMTRFMMSLDDAVDLVIYAYKNGRQGDIFIQKAPAATIGVLARAII
                     NIFK"
BASE COUNT          706 a          879 c          908 g          684 t
ORIGIN      
        1 acgctatggg gtgcactatg tttttgcttg ctgacacaaa atacttgacg ttacctgttc
       61 atctccctga ccacagccat tgcccctggt agactccgta tcataaccct gcgttccatc
      121 tggagtgcct ccttatcata gttctggacc gtggtgggtt tctcgctatg atggtacccc
      181 ggtttttgtt cctgacacaa agaatgatac cttatctgca gtcggtacga caccctttga
      241 attcttgaca gactgttgac aaactcctct tttcgtcatc cccgacctga tccggggtcc
      301 agagatctct ctcacccatc gtttttcctg gattccggcc tgcgccggaa tgacgacaga
      361 acgtgaaccc gtttcgaaaa cccacttaat caacagcccg ttttggagct cggaaccttg
      421 aaccgtcttc tcatccctct tgcaggaact ttcgcatggc cttataatgt acctcatcaa
      481 ggccatcgac gcttttgcgg tacgaaaact gggaaaggag gttgcggcta tggacaacga
      541 tgaaagactc aaaggctgga atccttatct tgccggtgcg ctgacggggc ttctcctcat
      601 cctttcggtc tgggtggcag gcaagtattt cggggcttcc acgacctttg tgaggagcac
      661 cgggctcgtc gaaaaggttt tctcccagga aagggtcgcg cagatggaat acttcatcaa
      721 ggagcttccc aagatcgact ggcagtggat gttcgttgtc ggcatattct tcggctccct
      781 aatcgcctcg acgacgtcga aatcctttcg gtggcagggc gttcccacga tgtgggaggc
      841 aaggttcggg ccaaataggg caaagcgtgc ggtgacagcc ttcctgggcg gggcgatagc
      901 catgttcggg gcgcgcctcg ccgacgggtg accgagcggc catgggctga gcggttcgct
      961 ccagctggct gtaagcggtt acatctccct gatctgtttc ttcgttggcg gcctcatagt
     1021 cgcccgcatc ctttatcagg gaggtgccaa aaaatgaacg atctcctcta tggcatcata
     1081 acaggcatca tcttcggctt tcttcttcag aaggcccggg tcatacgcta cgacaagcaa
     1141 ctggcggcct tacggctcat ggacatgacc atcgtcaagt acatgttcac aacggtgctc
     1201 gtagccatgg tgggcgtcta tcttctcaag gacctggggc tcgtaaaact ctccatcaag
     1261 acaacgatcc tggggggcaa cataataggc gggctcatct tcggcgcagg ctgggggctt
     1321 ctgggatact gccccgggac atccctcggc gcgctgggcg aagggcgctg ggattcaata
     1381 tgggggatcg tcggcatgct cgcgggcgcc gcggtctttg ccgaactttt tcctctcatg
     1441 aaaagaacgg tcctgacctg gggaaacctc ggtaagatca ccatcccggg catcctgggc
     1501 atcaaccact ggatcgtcat tgtaatagtg gtggtactgg cagtcctgat gttcaggttc
     1561 ttcgaaaaga aggggctgta acagacaggt tgtaggttat aggcaatggg tgacgggtga
     1621 tcgctgcaag gaccgtggtt ttccggacat tcttcccttc gtcattccgg cgaaggccgg
     1681 aatgacgaac aagagggttg gaggagtgac gaggatggac cggatgtaca acaatcgaat
     1741 gctcatgcaa gaatgctctt ttcccatgac ccataaccca ttacccataa cccggtcttt
     1801 taaaggagtc taccatgtcg gctgagcgtc ttgttgtgat cggcggggtt gccgcgggga
     1861 tgagcgcggc gagcagtttc aagagactga aacccgaggc ggaggcgatc gtcctgcaga
     1921 acgaccattt catctcctac ggggcctgca gtctgcctta ctacatatcc gacgatgtga
     1981 aagaaatcaa ccgcctcatc tccctgtccc cggaggtcgc gacgaaagag aggggcatca
     2041 ccgtgctcac ccgtcacgag gcgcggtcca tagaccccgc caggaaaacg gtgaccgccg
     2101 tggacctcga cgggaacaag gagaagacca tatcctatga ccacctcgtc gtcgcgacag
     2161 gaggactgcc ggtgcgccct cccttcccgg gaatagacct cggacacatc ttcaccctga
     2221 ggaccctcca cgacggtatc gagatcaaga ggttcataga ccggtggaca acgttcgaac
     2281 cctgcgtcgg acctcaatgc gtgtacgtga accctttcgg tgccgagaag agacccatgc
     2341 gggcctctat cgtcgggggc ggctccatcg gcatggagat gtgcgagtcc ctgaggaaaa
     2401 gacgggtcga ggtgaccgtc ttcgagaaga tggaccgcgt cctcggcacc atggacacga
     2461 gcataacgaa catcgtcgag gaaagactgg cggccgaggg cgtgaacctc atgaaagggg
     2521 tgtccgtcca gagcttcgaa ggagacagtg gctccgttac ccgcgttatc acggacaagg
     2581 gaacatttga caccgacatg gtcctgctgg tgatcggcgc aaggccgaac acgaagatcg
     2641 ccgtcgatgc cgggatcgag cttggcgcgg gcggggcgat aaaggttgat gatcatctga
     2701 ggaccaacat cccggacatc tatgccgccg gcgactgtgc cgaggccacg cagatggtca
     2761 ccggcaagaa agcctacatc cccctgggcg ccaccgccaa caagcagggt cgaatagcgg
     2821 gcgagaacgc cgccggcatg aacaacgcct ttgaaggcgt ggtgggaacg gccgtcacaa
     2881 agatcttcga tctcgaagtg gcccgcacgg gcctgtcacc cctcgaagcg gaaagggaag
     2941 gcatcgacca cttcgtgtcc accataaaag gcaggtcgag aagcaccgca tgccccgagg
     3001 gcaagcccat cacggtgacc gatccagaaa tgactagatt catgatgtcg ctcgatgatg
     3061 cggttgatct tgttatttat gcgtataaaa acggcaggca gggggatatc ttcatccaaa
     3121 aagctcctgc ggctaccata ggagttctgg cgcgtgctat aatcaacatt ttcaaat
//
LOCUS       DAOQTR010000240         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35275_length_3177_cov_3.099616, whole genome shotgun sequence.
ACCESSION   DAOQTR010000240 DAOQTR010000000
VERSION     DAOQTR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35275_length_3177_cov_3.099616"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKN85_06585"
     CDS             complement(<1..421)
                     /locus_tag="PKN85_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44110.1"
                     /translation="MKKFKILVTDHLAEEGLKILAADKAVEVEVRAGIKVEELKTIIG
                     NYDAIITRSGTTVKESLIENPGQLKIIGRAGVGVDNIDIEAASKKGLIVLNAPTGNTL
                     AATELTMGIMLAAARKIPLANESLKQGKWDRKKFMGIE"
     gene            533..1300
                     /locus_tag="PKN85_06590"
     CDS             533..1300
                     /locus_tag="PKN85_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44111.1"
                     /translation="MANEEPAEKRTSPDLNLEGERRSEGRRPLINRSNLRDIIIVVCL
                     ISAVLYDLDWVRLSVAGALLTFGCFFHYMTKGVLIRNVVLCREGTYGVVRHPYYMANY
                     LIDSSFCLVSGNVYLLLIYPFLFYWSYGPTIRKEEATLARLHDSEYLRYSLDVPQIFP
                     DAYSVRSMKGVGKGFSMQRITRNEISRFMRFWATGFLIIFIHTLKTTDMQRLWSFDLA
                     VVRDNPYGFALLLSTVILFVASLLVQCKKEESILKAE"
     gene            1305..>3177
                     /locus_tag="PKN85_06595"
     CDS             1305..>3177
                     /locus_tag="PKN85_06595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT44112.1"
                     /translation="MGPAVNPTPGQKWSFADYVALAVLVAAAAMLFFSGLSSRSLWGS
                     EGRWAVIAREMMQGGNYFLPTINGVVYFDKPLLSYWAIIPFSLFSGVTEASARIPGVL
                     AGMGTVALIFAIGRRLFGLASGFYAGLILLTTAMFGFWSRTASAEVLNVLAIWSMLWI
                     LQGRGAGRPFHRYLLFYLVAAVSSFCKGPLAPAVAVTAVAAVSCVGMIRDIRQKGFGG
                     APGKATDHFDWILSARGVLAAVCGLAAFALLLFLPVIMTGSWEAVELMWRENVVRFLE
                     PFDHVEPFYIYFKHTPVFLLPWTLLAIGAVIHMKRWGGGPERRWLATVTVAIFLFFTL
                     SGSRRSYYILPLVPAFALIIGRSLAGFLHRETEKDSPLMKVALVITAFIPVIAGAALI
                     AGFFGFEAYRHSSEIIAGPVIMMAGIAALFFLFKRMYVPGSALVVLLFFGLLLWTFTA
                     GTSIGERGRGLKPFARHVASRIEGVSDGNVAIYGVGNSSFIFYLDRKVPVRALSDVRQ
                     VCSFGDGVGRYLITEEAFVGTIEKECGSERFDRALSESRDDRTKRRNDLVLLVEEGRD
                     IQQQAVEFAAFFPAADRLVHRRADQRSVREGRAHRLAARDEIRGLGDDAFVAEVARRL
                     "
BASE COUNT          545 a          925 c          944 g          763 t
ORIGIN      
        1 gctcgatgcc catgaatttc tttcggtccc acttgccctg cttcagggat tcgttggcaa
       61 ggggtatctt gcgggcggcg gcgagcatga tgcccatggt gagctccgtg gcggccagcg
      121 tgttccccgt gggggcgttg aggacgatga ggcctttctt gcttgccgct tctatgtcga
      181 tattgtccac gccgacaccg gcgcgaccga ttatcttcag ttggccgggg ttctcgatga
      241 gactctcttt gaccgtcgtg ccgctgcggg tgatgatggc atcgtaattt ccgatgatgg
      301 tcttcagttc ttcgaccttt atgcctgccc tgacttcgac ttcgaccgcc ttgtccgctg
      361 caaggatctt gagtccttct tcagcgagat gatctgtgac gaggatcttg aattttttca
      421 ttggaatccg cctaagttat tagttcttgt aatatttctg tgctcgtgat atacgtaacg
      481 acagtaaaag ataccacccg caaacagaaa aagcaataca ggaccaatag ttgtggcgaa
      541 cgaggaacct gccgaaaaga gaacatcccc ggacctgaac ctggaaggcg agaggcgttc
      601 cgagggcagg aggccgctca tcaaccgcag caacctgcgc gatatcatca tcgtggtctg
      661 tctcatctcc gccgtactct acgacctcga ctgggtaagg ctttcggtgg cgggtgcact
      721 ccttaccttc ggctgtttct tccactacat gactaagggg gtcctcatcc gcaacgtggt
      781 cctgtgccgg gaaggaacgt acggcgtggt ccgtcatccc tactacatgg cgaattatct
      841 catagactcg agcttctgcc tcgtgagcgg caacgtttac cttcttctca tatacccctt
      901 cctcttctac tggtcctacg gcccgaccat cagaaaagag gaggctacgc tggccaggct
      961 ccatgacagc gaatacctgc gctacagcct cgatgtaccc cagatcttcc ccgatgctta
     1021 ctccgtccgc agcatgaaag gggtgggcaa gggcttttcg atgcagagga tcacccggaa
     1081 cgagatatcc cggttcatgc gcttctgggc gacgggtttc ctcatcatat tcatccacac
     1141 gctgaaaacg acggacatgc aaaggctctg gtcgttcgat cttgccgtcg tcagggacaa
     1201 cccgtacggt tttgcgcttc tgctctcgac cgtcatcctc ttcgtcgcca gccttctcgt
     1261 ccagtgcaag aaggaagagt caatcctcaa agcagaatag ccccatggga cccgccgtga
     1321 accccacacc cggacagaaa tggagctttg ccgattacgt ggctctggct gttctcgtcg
     1381 cggccgccgc catgcttttt ttttccggtc tttcttcgcg gtcgctgtgg ggttccgaag
     1441 gccgctgggc ggtcatcgcc cgggagatga tgcagggcgg caactatttc cttcccacga
     1501 tcaacggtgt cgtctatttc gacaaaccgt tgctcagcta ctgggcgatc atccccttct
     1561 cgctcttttc gggggtgacg gaagcctccg cccggatccc tggcgtgctt gcgggaatgg
     1621 gtacggtggc cctcatcttc gccatcggca gacgcctttt cggtctggca tcggggttct
     1681 atgccggcct catccttcta acgacggcga tgttcggttt ctggtcgagg acggcgtcgg
     1741 cggaggtcct gaatgttctg gccatatggt cgatgctctg gatcctgcag ggcaggggtg
     1801 ccgggcgccc gttccaccga tatctattgt tctacctggt agcggccgtc tcttccttct
     1861 gcaagggccc cctcgcgccc gccgttgccg taacggccgt cgcagcggtg agctgcgtcg
     1921 ggatgatccg ggacatccgg cagaagggat tcggcggggc cccgggtaag gcgacggacc
     1981 attttgactg gatactctct gccaggggcg ttttggcggc tgtctgcggc cttgccgcct
     2041 ttgccctgct ccttttcctg ccggtgatca tgaccgggtc ctgggaggcc gtggagctta
     2101 tgtggcgcga gaacgtggta aggttcctgg agcctttcga ccacgtggag cctttctaca
     2161 tctatttcaa gcacaccccg gtgttccttc tcccctggac gctccttgcc atcggggccg
     2221 tgatccacat gaagagatgg ggaggcgggc ccgagcggcg gtggcttgcg acggtgacgg
     2281 tcgccatatt tctcttcttt acactttccg gttcgaggag gagctattac atactgcccc
     2341 tcgtccccgc ctttgcgctc atcatcggga ggtctctggc ggggtttcta caccgcgaga
     2401 cggagaagga ctccccgttg atgaaggttg ccctcgtcat cacggccttt atccccgtca
     2461 tcgcgggagc ggcgctcatc gccgggttct tcggattcga agcataccgt cacagttcgg
     2521 agatcatagc gggaccggtg atcatgatgg ccgggattgc ggcgctcttc tttctcttca
     2581 agaggatgta tgtgcccggg tcggcgcttg tggttctgct cttcttcggg ctccttctgt
     2641 ggacctttac cgccgggacc tcgataggcg agagaggccg tggcctcaag cccttcgcgc
     2701 gtcacgtcgc gtcgaggata gagggcgtat cggatgggaa cgtggcaata tacggggtgg
     2761 gcaattcgtc cttcattttc tatctcgacc ggaaggtacc cgtgagggcc ttgagtgatg
     2821 ttcggcaggt gtgttctttc ggggacggcg tgggcagata cctcatcacc gaggaggcct
     2881 tcgtggggac catcgaaaag gaatgtggtt cggagaggtt tgacagggca ctcagcgagt
     2941 cacgggatga ccggacgaag aggcgcaacg acctcgttct cctcgtcgag gaaggacgcg
     3001 atattcagca gcaggcggtc gagttcgccg ctttcttccc cgctgcggat cgcctcgtgc
     3061 accgacgggc tgatcagcga agcgtccgcg aaggccgtgc tcatcggctc gccgcgagag
     3121 acgaaatccg cggccttggc gacgatgcct tcgtagcgga ggttgcccgc cgactgg
//
LOCUS       DAOQTR010000241         3164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35466_length_3164_cov_3.259891, whole genome shotgun sequence.
ACCESSION   DAOQTR010000241 DAOQTR010000000
VERSION     DAOQTR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35466_length_3164_cov_3.259891"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKN85_06600"
     CDS             <1..642
                     /locus_tag="PKN85_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNT44113.1"
                     /translation="YPVELNLLYENSTKRSCAAGEMIYIQSETSRPEFYLIEKGRVKI
                     ALLSKDGSERTVIIQERNTLFGYAAAFDGHPHFQTATALEPTELRVIPVATFLALNEK
                     HSRLSAVIISAFARVTRMLILQIESDSFMDAHRRVAHMLCKLASEVGQRTAKGILIAK
                     KVTQEDLGNLTGLSRVSVSLALNHFEEKDLLRKKRNMIEIFNLEELQTLAGLS"
     gene            775..960
                     /locus_tag="PKN85_06605"
     CDS             775..960
                     /locus_tag="PKN85_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44114.1"
                     /translation="MQVKMFYTAADVSALEKELNTWLSTRKISVNTIKQSYSCDNGTC
                     YTLISVWFESQESVAGI"
     gene            complement(1066..1407)
                     /locus_tag="PKN85_06610"
     CDS             complement(1066..1407)
                     /locus_tag="PKN85_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44115.1"
                     /translation="MFEVSQEASDRMKKFLQEQENPKPIRILTTEGGWRGLYLVMAMD
                     DQKETDEVLTDRGVTFVVDKKLFERAKPIRIDYTHSTLGEGYVFESQLLKGKEGLFTG
                     CRNICDTCEDG"
     gene            complement(1645..2103)
                     /locus_tag="PKN85_06615"
     CDS             complement(1645..2103)
                     /locus_tag="PKN85_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNT44116.1"
                     /translation="MIGVTMGAVGSLTGKDRSQCGLLIENGEGKVLLQLRDDNPDIPY
                     PGCWGTFGGQIEGDETPDEGIRREIWEELEYELSDPEYFGNFPFDGYDIYMYRTVDRG
                     LTLDDLTVREGQGGRFFSLTELEGVDCAANCREIVTAYFGMFHHGKSGKR"
     gene            2231..>3164
                     /locus_tag="PKN85_06620"
     CDS             2231..>3164
                     /locus_tag="PKN85_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNT44117.1"
                     /translation="MASMKSDTARYHYIDNLRTMVIMLVIMLHLAVTYSGLGRWYYNE
                     DHAPELFGLVFFSLFQTFVQGFSMGMLFLVAGYFTPGALERKGLGRFVKDRWRRLGLP
                     SLFYLLVVTPFICWTELPSSQWRSSASNFPDFYSGYLMSGGMKLLGVGPMWFAVALLI
                     FSIMYALLRAVRPPAADTGHGQGTGSFGVLAALILIVTLGAFLLRLAFPLGTIFWGMQ
                     LCYFSQYVILFIAGILAYRTRFQERITPAVGRRWLIAGIVLGLPLLFAIKWMAGVYHF
                     PVHPAHVRSAYANVAGGFSWFSFSFALWESFVAVS"
BASE COUNT          642 a         1007 c          836 g          679 t
ORIGIN      
        1 tatccggtcg agctgaacct gctttacgag aattcgacga aacggtcctg cgccgccggg
       61 gagatgatct acatacagag tgagacgagc cggcccgaat tctatctcat cgaaaaaggc
      121 agggtcaaga tagcgctcct cagcaaggac ggctcggaaa ggaccgtcat catccaggag
      181 cgtaacacgc ttttcggcta tgccgccgcc ttcgacggcc atccccattt ccagacggcc
      241 accgccctgg agccgacgga gctgcgcgtg atccccgttg ccaccttcct cgccctcaac
      301 gagaagcatt cgcggctgtc cgccgtcatc atctccgcct tcgccagggt gacgaggatg
      361 ctgatactcc agatagagag cgactccttc atggacgccc atcggcgggt cgctcacatg
      421 ctctgcaagc tcgcctccga ggttggtcag agaacagcga agggcatcct catcgcgaag
      481 aaggtgaccc aggaggacct cggcaatctg acggggctct cccgggtttc ggtctccctc
      541 gccctcaacc acttcgaaga gaaggacctc ctcagaaaaa agaggaacat gatcgagatc
      601 ttcaacctgg aagagctgca gaccctcgcc ggcctgtcat aagggaatcc cgggaggccc
      661 cgggatccgc cgcggcaaag gcgtgacgcc gatctccccg ttgccggcaa aacccaccgt
      721 gattagagtc tctcaaaagg caccagcaaa cgaaaacgcg aggagggaga agatatgcag
      781 gtcaagatgt tctataccgc ggcagatgta agtgctctcg aaaaagaact caacacgtgg
      841 ctgtcaacgc ggaagatctc agtgaacacg ataaagcaga gctactcatg tgacaacggg
      901 acatgttaca cgctgatctc cgtatggttc gaaagccagg aaagcgtagc cgggatctag
      961 agcaggcggg cggcatgcct cacaaaggcc ctgacaaacc cgcgatcgtt catgaccacg
     1021 ctcctttcag caccgcccga tctcccctga ggtcatcgcc gacccttagc cgtcctcaca
     1081 ggtgtcgcag atgttcctgc accccgtaaa aagaccttct ttccccttca ggagctggga
     1141 ttcaaaaacg taaccctcgc ccagggtcga gtgggtgtag tcgatccgta tgggcttcgc
     1201 cctctcgaaa agtttcttgt caacgacaaa cgtcacaccc ctgtccgtca ggacctcatc
     1261 ggtctccttc tggtcgtcca tggccatgac gagatacaac cccctccatc ccccttcagt
     1321 cgtcagtatc ctgatcggct tcggattctc ctgttcctgc aggaacttct tcattctgtc
     1381 agatgcctcc tggctcactt caaacatggg gacctcctca ttcatttgct ttctcacttt
     1441 ccgttccggg cccggtggga accggacgat accagcatct ctctcctgtt catattgtcc
     1501 ggcaatcaag gggccatttc aagacaaaag aggatggcgg aatgaaagtg gagccggcga
     1561 tccccacgcc gcaggtgccc catgagaccc taacactgta cttcgtaaac ccgatcgtgg
     1621 acaaaaggat cgcggccaag cgcgtcacct cttcccgctc ttgccgtgat ggaacatccc
     1681 gaaataggcg gtcacgatct cgcggcagtt ggccgcgcaa tcgacgcctt ccagttctgt
     1741 gagcgagaag aacctgcccc cctgcccttc cctcaccgtc aggtcgtcaa gggtgaggcc
     1801 cctgtcgacc gtcctgtaca tgtagatatc gtatccgtcg aagggaaagt tcccgaagta
     1861 ctccgggtcg gacagctcgt attcgagctc ctcccatatc tccctccgga tcccctcatc
     1921 gggggtctca tcgccttcta tctgcccgcc gaatgtcccc cagcacccgg gatagggtat
     1981 gtcaggatta tcgtccctga gctgtaacag gaccttgcct tccccgttct cgatgagcag
     2041 gccgcactgg cttcggtcct ttcccgtaag agatcccaca gcccccatgg taacacctat
     2101 cacgacaccg cgtcaaaaca aagcggagag acggcatgtt gtcttgacag atgccggccc
     2161 accctactat agtgtggtag cttatgctca tcaatcactc ttccgataat ccccgcaggg
     2221 tcggtccctt atggcctcga tgaagagcga tactgccaga tatcactaca tagacaacct
     2281 gcggaccatg gtcatcatgc tggtcatcat gctgcacctc gccgtgacct acagcggcct
     2341 cggcaggtgg tattacaacg aggaccacgc gccggaactc ttcggtctcg tcttcttcag
     2401 tctcttccag acattcgtgc agggtttctc catgggcatg ctgttcctgg tggccggcta
     2461 tttcacgccg ggcgccctag agcgcaaggg tctcggccgt ttcgtaaagg accgctggag
     2521 gcggctgggt ctgccctcgc ttttctatct gctcgtcgtg acccctttca tatgctggac
     2581 ggaactgccg tcgtcgcaat ggagaagcag cgcgtcgaac tttccagact tctacagcgg
     2641 atatctaatg tccggcggaa tgaagctcct cggcgtcggg cccatgtggt tcgcggtggc
     2701 gctgctgatc ttttcgatca tgtacgccct cttgcgggcg gtccggccgc cggccgcaga
     2761 caccgggcac gggcagggca cggggagctt cggcgtactg gccgcgctca tcctgatcgt
     2821 caccctcgga gcattcctgc tgaggctcgc cttccctctg ggcaccatct tctgggggat
     2881 gcagctctgc tacttctcgc agtacgttat cctcttcatc gccgggatac tcgcctatcg
     2941 tacaaggttc caggaacgga taacaccggc cgtcggcagg aggtggctga tcgccggcat
     3001 cgtcctcggt cttccactcc tcttcgcgat caaatggatg gccggggtct atcatttccc
     3061 ggtacatccc gcccatgtga ggagcgccta cgcgaacgtc gcggggggat tctcctggtt
     3121 cagtttttcc tttgccctgt gggaatcctt cgtcgccgtt tcca
//
LOCUS       DAOQTR010000242         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35613_length_3155_cov_3.564839, whole genome shotgun sequence.
ACCESSION   DAOQTR010000242 DAOQTR010000000
VERSION     DAOQTR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35613_length_3155_cov_3.564839"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PKN85_06625"
     CDS             complement(<1..1351)
                     /locus_tag="PKN85_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44118.1"
                     /translation="MKKKMALNTRHLSGKAVFAVLLLGMLILSGCATPVGVGYLDPRE
                     SHRKLTANVLTGSTLSAPTMQMLNRTGLAERFKTHPAEVIAAIHKGVPTADETDRIFA
                     LAELSFLYASQSADRSYYLASAVYAWALLFPEGGARAPDAFDPRFRTAVDIYNLGIAE
                     GLTSADGVVIDLKEGTHKLPFGEVAISVRADSFRWSSYRLVRFADASKLSVRGLRNEY
                     RWPGIGAALVASTEYLPGAADPVSAKVPFDIKVAVTLLVRFHNVDEGVRTGRMRGRLE
                     LYTTDSATTVTVGGRTVPLEFDISSALAYSLENSGVYRMETKGFFSGNVTLFKDASRF
                     KEHIFFMSPYIPGRIPVIFIHGTASSPARWAEMLNELQNDRRLWGRYQFWAFTYNTGN
                     PILYSGGLLTEGLKELVRGLDPEGRDPALRRMVIVGHSQGGLLTRLTVVDSGTRFWED
                     "
     gene            complement(1397..2410)
                     /locus_tag="PKN85_06630"
     CDS             complement(1397..2410)
                     /locus_tag="PKN85_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNT44119.1"
                     /translation="MSIRSRNTFPGRFFRRIGNVFRRFAFLIMTAWATLAVYYSDLPP
                     SFRLIAAGLFVIGGLAVLTVGYRRRAARFVFLAAFAAVLVWWLLIPPSNTRNWQPDVA
                     LLPWAAIDGDRVTIHGIRNVDYRTETDYTVRHYDRTFDLTKMKSVDLFVVYWGSPLIA
                     HTMLSFGFAGGDQVCFSIETRKEVGEEYSSIKGFFKQFELTYIIGDERDLVRLRTNYR
                     KEQVYLWRLRTSMDLARNVFLDYLRTVNLLRDRPEWYNALTSNCTTNIRGHTVPYSPN
                     ARFDWRIIINGFIDRMIYDRGMVDRSLPFDEIKRRSRINEKAQAAGNDPAFSRLIRQG
                     LPR"
     gene            complement(2487..3071)
                     /locus_tag="PKN85_06635"
     CDS             complement(2487..3071)
                     /locus_tag="PKN85_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44120.1"
                     /translation="MKRTLSVVLASLLVVFIASVGFSEEKKAAKKAGPKPAGAMVDVI
                     EMTAVIDAVDAAKRTVTFKMPDGTARAFKVGPEVKNFDQIKAGDKVKTTFYESIAVFV
                     AKTGEKPSATEFGTVEVAPKGAKPGAVVVDTLEVTAKVEKIDYKKRTISLKGPEGNVR
                     TFIVDKSVKNFKNIKKGDDVYLRVTEAVAMVVEK"
BASE COUNT          645 a          956 c          916 g          638 t
ORIGIN      
        1 catcctccca gaagcgcgtc ccgctgtcga caacggtcag tctcgtcagg agccctccct
       61 ggctgtggcc cacgatgacc attcgcctca gcgccgggtc cctgccctcg gggtcgagtc
      121 cgcggacgag ctctttgaga ccctctgtca ggagcccgcc cgaatagaga atggggttgc
      181 ccgtgttgta ggtaaaggcc cagaactggt accggcccca gagtcgccgg tcattctgca
      241 gttcgttgag catctccgcc cagcgggcgg gactcgaggc cgtcccatgg atgaagatga
      301 cggggatgcg ccccggtata taggggctca tgaagaagat gtgttccttg aaccgcgacg
      361 cgtccttgaa aagggtcacg ttcccggaaa agaacccctt cgtctccatg cggtacaccc
      421 ccgagttctc cagggagtaa gcaagagccg aggaaatgtc gaactccagg ggtaccgtgc
      481 gccccccgac cgtgacagtg gtggcgctgt ccgtcgtgta cagttccagc cttccccgca
      541 tcctgcccgt ccttacgccc tcgtccacgt tgtggaaccg gacgagcaag gtaacggcga
      601 ccttaatatc aaaagggacc ttcgcggaga cggggtccgc ggcaccggga aggtattcgg
      661 tggaagcaac gagcgccgcg cctatccccg gccaccggta ttcgttgcgc aatccccgga
      721 cggaaagttt tgacgcgtcg gcaaacctga cgaggcgata tgagctccac cggaacgaat
      781 cggcacggac cgagatcgcg acctccccga acggcagttt gtgcgtacct tccttaaggt
      841 cgatgacaac cccatcggcg gaagtgagac cctcggctat cccgaggttg tagatatcca
      901 ccgccgttct aaaacgggga tcgaaggcat cgggcgcgcg ggcaccgcct tcggggaaga
      961 gaagcgccca ggcatacacg gccgaggcaa gatagtacga tctgtctgcg ctctgggaag
     1021 cgtacagaaa ggagagctcc gccagggcga agatgcggtc cgtctcatcc gcggtcggga
     1081 cacccttgtg tatggcggcg atcacctcgg ccggatgggt cttgaatctc tccgcaagac
     1141 cggtccggtt gagcatctgc atggtcgggg cgctgagagt ggacccggtc agcacattcg
     1201 cggtaagctt gcgatgggat tcccgcgggt cgaggtaacc cactccgacc ggagtggcac
     1261 accccgacag gatgagcatg cccagaagga ggaccgcgaa gaccgccttc ccggaaagat
     1321 gtcgtgtgtt cagggccatc ttcttcttca taccattatt cacgaagtca tcacaaacga
     1381 aaaagcctcc cggccgctac ctgggcaagc cttgacgaat gagccgggag aacgccgggt
     1441 cgttgcctgc cgcctgcgcc ttttcattga tacggcttcg tcttttgatc tcatcaaagg
     1501 gaaggctcct gtccaccatg ccccggtcgt atatcatcct gtctatgaaa ccgttgatga
     1561 tgatcctcca gtcgaagcgc gcgttgggag aatagggcac cgtgtgaccc cggatgttgg
     1621 tcgtgcagtt gcttgtgagg gcgttgtacc attcgggacg gtcccgcagg aggttcacgg
     1681 tgcgcaggta atcgagaaag acgttgcgcg cgagatccat cgatgtcctc aggcgccaca
     1741 ggtagacctg ttccttgcga tagttcgtcc tcaggcggac cagatcgcgc tcgtcgccga
     1801 tgatgtaggt aagctcaaac tgcttgaaga acccttttat ggaagagtac tcctctccga
     1861 cctccttgcg cgtctcgatg gagaaacaga cctgatcccc cccggcaaaa ccgaagctga
     1921 gcattgtgtg ggcaatgagt ggtgaacccc agtagacgac gaaaaggtcg acgctcttca
     1981 tctttgtcag gtcgaaggtc ctgtcgtaat ggcgcacggt gtaatccgtc tctgtcctgt
     2041 aatcgacatt gcggatgccg tggatggtca ccctgtcgcc atcgatcgcg gcccagggga
     2101 gaagggcaac gtcaggctgc cagttccttg tattcgacgg cgggatcagg agccaccaga
     2161 ccagaacggc cgcgaaggcg gcaagaaaga caaaccgagc ggcccgacgg cgatacccca
     2221 cggtgaggac ggcgagaccc ccgatgacaa aaagtcctgc agcgatgagt ctgaaagagg
     2281 gaggcagatc ggagtagtag acggcgagcg ttgcccaggc cgtcattatc agaaatgcga
     2341 acctcctgaa gacattgccg atgcgtctga agaaccggcc gggaaatgtg tttctggaac
     2401 gtatgctcat gggaatgcag gaaaccgccc gcgcatggcc gcgggcggtc cccgatgatc
     2461 ctgcgttatc tttggtcccg caaggtctac ttctccacca ccattgccac ggcctcggtc
     2521 actctcaggt agacgtcgtc tcccttcttg atgttcttga agttcttgac ggacttatcg
     2581 acgatgaagg tccgcacgtt tccttcgggg cccttgagag aaatggtacg tttcttataa
     2641 tctatctttt cgaccttcgc cgttacctcc agcgtgtcaa ctaccacagc tccgggcttc
     2701 gcgcccttcg gcgccacttc cacggtcccg aactccgtgg cggaaggctt ctcgcccgtc
     2761 ttcgccacaa agacagcgat cgattcatag aaggtcgtct tgaccttgtc gccggccttt
     2821 atctggtcga agttcttcac ttccgggccg accttgaagg cacgcgccgt gccgtcgggc
     2881 atcttgaaag tgaccgttct cttcgccgca tccacggcat cgataacagc cgtcatctcg
     2941 atgacgtcca ccatagcccc ggcgggcttg gggccggcct tcttcgccgc tttcttctcc
     3001 tccgaaaacc cgacgctcgc gatgaagacc acgagcaacg acgccagtac cactgacagt
     3061 gttcttttca tatacatcct cctctgggat atttcaggcc gcccggcggc ctgtgtattt
     3121 caggtgatca cgaatccgaa cacctcaccg cgaac
//
LOCUS       DAOQTR010000243         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_35789_length_3144_cov_3.630625, whole genome shotgun sequence.
ACCESSION   DAOQTR010000243 DAOQTR010000000
VERSION     DAOQTR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35789_length_3144_cov_3.630625"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKN85_06640"
     CDS             <1..276
                     /locus_tag="PKN85_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT44121.1"
                     /translation="RGVEVYGHKGPKDASFLATAAFNIKGLLPSDVGYELNRRSMYVR
                     IGLHCAPMAHQTVGTFPNGSLRASPGYFTQEDDIRAFIEAVSEIAEG"
     gene            811..1179
                     /locus_tag="PKN85_06645"
     CDS             811..1179
                     /locus_tag="PKN85_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT44122.1"
                     /translation="MNRVLSRFVSPSTGDFPAVNIWSDGEETLVTTEIPGIESGAIDI
                     SVAGKTLILKGAREPDQVEEGSSYHRRERWYGQFSKAIELPFTIDADRVAADFSNGIL
                     TIRLPRSEAEKPRKITIKSA"
     gene            1191..1595
                     /locus_tag="PKN85_06650"
     CDS             1191..1595
                     /locus_tag="PKN85_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT44123.1"
                     /translation="MADKTKDIPKKEAAETDVVERTRAGKIYNPDVDIMEGKDKIIVI
                     ADMPGVNENSVEVTLDDNVLTIYGRVDWKAPEKMKLIHAEYGVGDFQRIFTISGEINR
                     EKIQARVKNGVLRLVMPKNDAPKMRKIAVKAG"
     gene            1628..2128
                     /locus_tag="PKN85_06655"
     CDS             1628..2128
                     /locus_tag="PKN85_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT44124.1"
                     /translation="MTTRRVSSSVKRARGAARLSEENPLLSLRSNIDRLFDNFLSGLN
                     ISPFTATASMFNPSIDIADSGKELRVTVELPGMDENDIDVSVTQTSITIRGEKRDETE
                     EKGNSYHRMERVYGSFTRTVPLPVEVNVEDARADYKKGVLSIIIPKTEKALKEAKKIP
                     VKASAK"
     gene            2167..2349
                     /locus_tag="PKN85_06660"
     CDS             2167..2349
                     /locus_tag="PKN85_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44125.1"
                     /translation="MMAFPATGPDLPGPRLPPDNIADEWMERILETPGMLEDFTVFLL
                     IVFLLETRNMEPETRL"
     gene            complement(2346..>3144)
                     /locus_tag="PKN85_06665"
     CDS             complement(2346..>3144)
                     /locus_tag="PKN85_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT44126.1"
                     /translation="RIGGYVDMLRELGVRAVPFTREVAKKGSGISPYREFVFGEDPDR
                     YEAVINVPKLKTHSMMGMTLGVKNTFGFIRGFAKGRWHLRAGRDRDLFASIIVDIHRI
                     ASPTLTVLDGILGMCGDGPSNGDPVACRMVAVSRNAFALDAFIEDRLWPGMPLPITEC
                     ASRHGLVPPYEVIDLGLPPAPTPFTVPRGCDTDWNLPRPVKRLLRNVLTKKPKVDQDA
                     CRLCGICARVCPADAIRLGERSPLFDYRACIRCYCCQEMCPHAAIRT"
BASE COUNT          773 a          870 c          915 g          586 t
ORIGIN      
        1 cgaggtgtcg aggtgtacgg tcacaagggt ccgaaagacg catcgttcct ggcaaccgcg
       61 gcattcaaca tcaaaggtct gctcccttcc gacgttgggt acgaactcaa caggaggtcg
      121 atgtacgttc gcatcggcct tcactgcgcg ccgatggcgc accagacggt cggcaccttc
      181 ccgaacgggt ccctccgggc atcaccgggg tatttcaccc aggaggacga cataagggca
      241 tttatcgagg cggtcagcga aattgccgaa ggctaagggc ttcattctct tatttcgttc
      301 gatccacgat gtcctgcgag cggaaaaaat gctcaaaaag caggacgtga tacacgaact
      361 cgtaccggta ccaagaaatt tgagttccga ctgcggcatg tgtatcaggc ttgacagcga
      421 tgcggcagat atacaacggt gcctgggtga tattgagatc gacaggagct ttgtcttcga
      481 cggcgagagc taccaacagg tcgaactatg atagccgcgg tcgtcgacgc ctgccccgtg
      541 gtccctgtat ccctgccgct tgccctccag gcgggaaacg ggcagcaccg aacgtggcca
      601 cctcctttcc agctgtacca ataacctatt agattcatcc acttagaacc catccacgcc
      661 acttgacaaa agatatccga gtgaacagat taatttcaca aagaggaaat atgcaacaag
      721 gaggctgata ccatgttgtg gacagacggt tttggcatgt ttggcagagg gcttgacccg
      781 tggtccgagt ttcaacggat cgagaacgag atgaaccgcg tcttatccag gtttgtctcg
      841 ccctcgacag gtgactttcc cgccgtcaac atatggagcg atggcgagga gaccttggtg
      901 acaacggaga tcccgggtat cgaatcaggc gcgatagaca tctcggtcgc aggcaagacc
      961 cttatactca agggtgccag ggagcctgac caggtggagg agggtagttc ctatcatcgc
     1021 cgcgagcgat ggtacggcca gttcagcaag gccatcgaac ttcccttcac gatcgacgcc
     1081 gaccgggtcg cggccgactt ctccaatggc atcctcacga tcagattgcc gcgatcggag
     1141 gcggagaaac cgagaaagat cactatcaaa tccgcgtaag gaggcgcacg atggctgaca
     1201 aaaccaaaga cataccaaag aaagaggccg ccgaaacgga tgtggtggag aggacccgag
     1261 cgggcaagat ctacaacccc gatgtcgaca tcatggaagg taaggacaag atcatcgtga
     1321 tcgccgacat gcccggagtc aacgagaact ccgtggaggt tacactggac gacaatgtcc
     1381 tgaccatcta cggaagggtt gattggaagg cgcctgaaaa gatgaaactt atccacgcgg
     1441 aatacggagt gggcgacttc cagaggatct tcaccatatc gggcgagatc aacagggaaa
     1501 agatccaggc aagagtgaag aacggtgttc tcaggctggt catgcccaag aatgacgctc
     1561 cgaagatgag gaagatcgct gtcaaggctg gttgaacaat gacgtgagac acacaggagg
     1621 tacgatcatg acgacaagaa gagtgtcatc gtcggtgaag agagcacggg gcgccgcccg
     1681 gctgtcggaa gagaatcccc ttctgtcgtt gaggagcaac atcgacaggc tgttcgataa
     1741 tttcctctca gggttgaata tcagtccttt caccgcaaca gcatcgatgt tcaacccgag
     1801 catcgatatc gcggacagcg gcaaggaact cagggtgacc gtcgaactgc ccggcatgga
     1861 cgaaaatgac atcgatgtat ctgtcaccca gacctctatc accattcggg gagagaaaag
     1921 ggacgagaca gaggagaaag gcaatagcta ccatcgtatg gaacgggtct acggctcttt
     1981 cacgaggacc gtcccccttc ccgttgaggt gaatgtggag gacgccaggg ccgactacaa
     2041 gaagggtgtc ctatccatta tcattccgaa gacggagaaa gccctcaaag aggcaaagaa
     2101 gatccccgta aaggcatcag cgaagtagag tacccgtcat acatcgcagg tcagaggccc
     2161 aatgtcatga tggcgtttcc cgcgacgggc cctgacctgc ccggcccccg gctccctccc
     2221 gacaacattg cagacgaatg gatggagagg atactggaaa cacccggcat gctcgaggac
     2281 tttacggtct ttctccttat tgttttcttg cttgaaacgc gaaacatgga acctgaaacc
     2341 cgcctctagg ttcttatcgc cgcgtggggg cacatctcct ggcaacaata gcagcggatg
     2401 cacgcccggt agtcaaagag aggcgatctt tcgccgaggc gtatggcgtc ggcggggcag
     2461 accctggcgc agatgccgca aagccggcac gcatcctgat ccactttcgg tttcttcgtg
     2521 agaacatttc tcaggagtct cttgacgggg cggggcagat tccaatccgt atcgcacccc
     2581 cggggcacgg tgaacggggt cggcgcaggg ggcaggccga ggtcgatcac ctcataagga
     2641 gggacgagac cgtgacgtga cgcgcattcg gtgatgggca ggggcatacc gggccagagg
     2701 cggtcctcga tgaaggcatc gagcgcgaag gcattccgcg agaccgccac catacggcag
     2761 gcaacggggt caccgtttga cggaccgtcg ccgcacatgc ccaggatccc gtcgagaacc
     2821 gtaagcgtcg gcgaagcgat acgatggatg tcgacgatga tggaggcgaa gaggtccctg
     2881 tcccgtccgg cgcgaagatg ccatcgcccc ttggcgaacc cccggatgaa cccgaaggtg
     2941 ttcttgacgc cgagggtcat ccccatcatg ctgtgggtct tgagcttcgg cacgttgatg
     3001 acagcctcgt acctgtcggg gtcctcaccg aaaacaaact ccctgtacgg cgatatcccg
     3061 cttcctttct tcgcgacctc cctcgtgaag ggaaccgccc gaaccccaag ctcccggagc
     3121 atatccacgt acccaccgat cctg
//
LOCUS       DAOQTR010000244         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_36334_length_3110_cov_3.769558, whole genome shotgun sequence.
ACCESSION   DAOQTR010000244 DAOQTR010000000
VERSION     DAOQTR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36334_length_3110_cov_3.769558"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PKN85_06670"
     CDS             complement(<1..966)
                     /locus_tag="PKN85_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNT44127.1"
                     /translation="MIQTVFGEILKVNYVVDQRVKGRVTFRSVAPVTLDQVLPIMETI
                     LRINGVGVVEESGLYRVVPISDVAKEPAQVKFGREPEKVLIQGKSIIQVVPLLHVHSS
                     ETIKFITPFLTATAVVIDVPNINYIILVDTDSNVKRLLTLISFFDGEQTKQKKPKVSV
                     YNIQNGKAKDVAAILQQVLLSAKPPQAPTATATSAPATGSATPGTPPPAQPQAGRPSL
                     QAAGTQGGDTLVSPITKIIADEGLNALIILSTQEDYEFIRAAIERIDVIPRQVLLEGV
                     VAEVSLKDELKLGISWALQFNPGGMRGVLGGVEGTVGFNVPGATPT"
     gene            complement(1561..1986)
                     /gene="gspG"
                     /locus_tag="PKN85_06675"
     CDS             complement(1561..1986)
                     /gene="gspG"
                     /locus_tag="PKN85_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNT44128.1"
                     /translation="MRKILNRQNRKGFTLIELLVVMVIVGLLAALVAPRLFPKLGKGK
                     QAAAKAQIELLGQALDQMRLDVGRYPTTQEGLNMLAVNPGVEGWDGPYLKKALPNDPW
                     GKPYNYQSPGSHGEYDLISYGRDGSPGGEGEDADVTSWQ"
     gene            complement(2358..>3110)
                     /locus_tag="PKN85_06680"
     CDS             complement(2358..>3110)
                     /locus_tag="PKN85_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT44129.1"
                     /translation="AEPALWQAAIIGAFSGAIRETKGLFPQAHGGTIFLDEIGDMPLS
                     IQAKILRVIQERKIRPVGSEKVIDVDVRIVTATNKNLEEQVRLDLFREDLYYRIHVIP
                     IQLPPLRERKEDIPPLVDFFLQKAGALMRKDIKGVTPEAMQKLMLYDWPGNIRELENV
                     IEYAAAMTDREYITEDLVFQTKGVVSQEPLRPLREAKEAFEKSYLIHLLEICKGNITE
                     VAKCAGKYRADLYDLLRKHNLRPEDFKKRESA"
BASE COUNT          538 a          933 c          943 g          696 t
ORIGIN      
        1 cgtcggcgtc gcaccgggca cgttgaaccc caccgtgccc tcaaccccgc ccaggacgcc
       61 tctcatgccg ccggggttga actggagcgc ccaggatatg cccagtttca gttcatcctt
      121 caggctcacc tccgccacca cgccctcgag aagcacctgc cgcggtatca cgtcgatcct
      181 ctcgatcgcc gcccggatga actcgtagtc ttcctgcgtg gacaggatga tgagagcgtt
      241 gagcccttcg tcggcgatga tcttcgtgat cggcgatacg agcgtatctc ccccctgcgt
      301 gccggcggcc tgcaatgaag gacgtcccgc ctgcggttgg gccggcggag gcgtgccggg
      361 ggtggccgaa cccgtcgccg gggctgaagt ggccgtagcg gtgggggcct gaggaggttt
      421 cgcggataaa aggacctgct gcaggatggc cgccacatcc ttcgccttac cgttctgaat
      481 attgtacacg gagactttcg gctttttctg cttcgtctgc tcgccatcga agaagctgat
      541 aagcgtgagg agccgcttca cgttcgaatc agtgtccacc aggatgatgt aattgatgtt
      601 gggtacgtct atcaccacgg ctgtcgccgt gagaaagggg gtgatgaatt tgatggtctc
      661 cgacgagtgc acgtggagca acggcaccac ctgtatgatg gacttcccct ggatgagcac
      721 tttctccggt tctcgtccga acttaacctg ggccggctcc ttcgcgacat cgctgatggg
      781 caccacccgg tacaggccgc tctcctcaac aacaccgacg ccgttgatac gcaggatcgt
      841 ctccatgatg ggaaggacct ggtcaagggt tacgggggca acggagcgga aggtcaccct
      901 ccccttcacc ctctggtcga caacataatt cactttcagt atctctccaa aaacggtctg
      961 tatcaccgag aaaacatcgg cgtcgtcgaa gttgaggctg atccggcccg ccgcgggagg
     1021 acgcgcgggc tgaaccctct ggggctgctg cggccggggt ggctgaaaag aagggggctg
     1081 ctgcggccgg ggtggctggc ccaccctggg gggggtcggt tgcacggcgg gtgcggtctg
     1141 ttgcgcggtg ggggtggcct tcgccgcccc gggccccggg ggtggagacg gggccggggg
     1201 ttttgaaggt gaagccggcg cctcattggc ctcgggcgcc gatcgggtgg tctgtggcgc
     1261 ggcggggaca gcctgtaccc gcgcgggggt cgttgtttgc gccgcaggcg cagcctgctg
     1321 tgccgtcggg gtggcctgtg gcgcggcgga tgcgtcagac gggaccgatc gcaccgcctg
     1381 cggggatgtc tcgagctgga tagcccgcgt gaccggaggc gggtcctcgt tcagggggcg
     1441 tgtcgtgatg gcgtcctcgg aggcgaacgc catcggtccg agacacccca gaatgaaagg
     1501 aacgatcatt atcgccggat atctcatggt ccgggccttg tccttgtgct catatcacgg
     1561 ctactgccag ctcgtcacgt ccgcgtcttc gccttctccg ccgggcgaac catccctccc
     1621 gtaggagatg aggtcatact ccccgtggct ccccggggac tgatagttgt atggtttacc
     1681 ccaggggtca ttgggcaggg cctttttaag atagggcccg tcccacccct ccactccggg
     1741 gtttacggca agcatgttga gaccttcctg cgtcgttgga tatcgtccga catcaagcct
     1801 catctggtcc agggcctgcc cgagaagctc tatctgggcc ttcgcggcgg cctgttttcc
     1861 tttccccagc ttgggaaata gcctcggcgc caccaaagcc gcgagcagac ccacaatgac
     1921 catgacgaca agaagctcga tgagcgtgaa acctttcctg ttctgcctgt taagaatctt
     1981 cctcatctgg gacccccggt atggcctccc gggcgaccac aggtccgtcc cggaaaagag
     2041 ttcctgacgt gccagcctat atctgcagga tacaaaatag ccctccttct gtatgtcata
     2101 aaaacgacaa ttatcttgta gtgcatccac catctcctgt gtcatcgttt tcgattacaa
     2161 tacccgatga gagggccgcg gaggagcacg aagaaggata ggacctgtag gagagatagg
     2221 acatatagga cttttcaggc cggagttggc cgaccggcac gtcctattgg tcctatttcg
     2281 tcctattggt cttatatgtc ctattggtcc tatctctctt acaggtccta tcatctcttt
     2341 cccctctgcc ttttcttcta cgccgactcc ctcttcttga aatcctccgg tctcaggttg
     2401 tgcttcctga ggaggtcgta gaggtcggcc ctgtacttgc ccgcgcactt cgccacttcg
     2461 gttatgttgc ccttgcatat ttcgagaagg tggatgagat agctcttctc gaaggcctcc
     2521 ttggcctccc tgagaggtct gagcggttcc tgggaaacga cgccttttgt ctgaaagacg
     2581 aggtcctccg tgatatactc cctgtcggtc atggcggccg cgtactcgat cacgttctcc
     2641 agttcccgga tgtttcccgg ccagtcgtag agcatcagct tttgcatggc ctcgggggtc
     2701 acgcccttga tatccttcct catgagcgca ccggcctttt ggagaaagaa gtcgacgaga
     2761 gggggaatgt cctctttcct ttcccgtaac gggggcagct ggatggggat cacgtggatc
     2821 ctgtagtaaa ggtcttcccg gaagagatcg agccgcacct gttcctcgag gttcttgttc
     2881 gttgccgtta cgatcctgac atcgacgtcg atgacctttt cgctgccaac gggacggatc
     2941 ttcctctcct ggatgacgcg cagtatctta gcctgaatgg agagaggcat gtcgccgatc
     3001 tcatcgagga agatggtccc gccatgggcc tgcggaaaaa gccccttcgt ttcgcggatg
     3061 gccccgctga aggccccgat gatcgccgcc tgccagaggg ccggctccgc
//
LOCUS       DAOQTR010000245         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_36412_length_3106_cov_4.295641, whole genome shotgun sequence.
ACCESSION   DAOQTR010000245 DAOQTR010000000
VERSION     DAOQTR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36412_length_3106_cov_4.295641"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKN85_06685"
     CDS             complement(<1..586)
                     /locus_tag="PKN85_06685"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT44130.1"
                     /translation="MRYKIFFFVLALFLVFYFYIAHLNPENVKFYYGGARPIELSLAE
                     FIIASFCIGVIISIIISFFYDVKTTITSWMARRSERKSEEYLGLLEKARHYDLKGDRE
                     KAIEHLDRLSRRNPGREETYIALADIYVSMEDYEKARETLDLAETTLGKTENVLFKKV
                     KVNIASRDYNKNEGILKEILRINESSLKAARLLRD"
     gene            complement(590..943)
                     /locus_tag="PKN85_06690"
     CDS             complement(590..943)
                     /locus_tag="PKN85_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNT44131.1"
                     /translation="MNKEKVEFYRKTLLKMRENILNKAKKLKEDSYNLGTDGIQDMAD
                     AASNTYNADILMSISDNDLTILKDIDNSLDKLSKGSYGICEECEEKISEKRLEANPVA
                     RYCITCKRQMEEKGL"
     gene            complement(936..1295)
                     /gene="rsfS"
                     /locus_tag="PKN85_06695"
     CDS             complement(936..1295)
                     /gene="rsfS"
                     /locus_tag="PKN85_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNT44132.1"
                     /translation="MVEACGRLADDKKALDVVVMDLTGITDIADHFVIAGGTSERHVR
                     TIADGIREGMKQEGARPLAVEGYDEGRWVILDYQSVVVHIFLEQLRELYDLESLWIEA
                     KRHRIKNENKNTEVGNE"
     gene            complement(1297..1944)
                     /gene="nadD"
                     /locus_tag="PKN85_06700"
     CDS             complement(1297..1944)
                     /gene="nadD"
                     /locus_tag="PKN85_06700"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNT44133.1"
                     /translation="MATGVFGGTFNPVHVGHLRVAEEIREDFHLDSVYFIPSHVPPHK
                     EAAGGLPGERLRMLKAAVRGNASFKVSDMEIRRGGVSYTIDTLKRLERRFDDIHFIIG
                     VDAFRQIDTWHHYEELFYHTGFIVMTRPASLPADIPEMVPEAVRVKLTALGDGVYRHE
                     SGKKIYLRAVTQMDISSTKVRDLLRGERSIRYLVPPGVEKIIIERGLYRSSDETR"
     gene            complement(1955..>3106)
                     /locus_tag="PKN85_06705"
     CDS             complement(1955..>3106)
                     /locus_tag="PKN85_06705"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNT44134.1"
                     /translation="LAEALGKNQDYLYEENMKDVRAAEKEGMAKSLVDRLRIDDKLIR
                     EMQGSLRDVAALPDPVGEIVKVWKRPNNLLVGRMRIPIGVILIIYESRPNVTIEAFSL
                     CLKSGNCVILKGGSEAYHSNRALYRLIVETLKGSKISPDAVQFVDTADRDYIYTLLER
                     EEEIDLVIPRGGEALIRNIVERSRIPVLKHYKGVCHIYVDDEADLTMAVDVAVNAKVQ
                     KPATCNALETLLVHEKVAKSFLPKVHRELSRQGVTMKGCPKTVAILKDIGRATKEDWP
                     MEYLDLTLAVKVVADMDEAVDHIRKYGSSHTEAIVTANYERAWKFLREVNSSLVLVNA
                     STRLNDGFQLGLGAEMGISTTRLHAFGPMGLEELTVTKFIAFGDGQLRT"
BASE COUNT          564 a          915 c          841 g          786 t
ORIGIN      
        1 ggtcccgaag tagtcgcgcc gccttgaggc ttgattcgtt tatccgcagg atctctttca
       61 atatgccttc gttcttgttg taatccctgg aggcgatgtt caccttcacc ttcttgaaaa
      121 ggacgttctc cgtcttcccc agggtcgtct ccgcgagatc gagggtctcc ctcgccttct
      181 cgtaatcttc catggaaacg tagatatcgg caagggcgat gtatgtttcc tcgcggcccg
      241 ggttgcggcg cgagagcctg tcgagatgtt cgatggcctt ttcccggtcg cccttgaggt
      301 cgtagtgccg ggccttttcc aggagcccca ggtactcctc gctcttccgc tcggagcgcc
      361 tggccatcca cgatgttatc gtggtcttca catcatagaa aaaactgatg atgatcgata
      421 tgatgacgcc gatacagaag gaggctatga tgaactcggc cagcgagagc tctatcggcc
      481 gtgcccctcc gtaatagaac ttcacattct cggggttcag atgcgcgatg tagaaataga
      541 agacaagaaa aagcgccaga acgaaaaaga atatcttgta ccgcatccgc tataaaccct
      601 tttcctccat ttgtcgctta caggttatac aataccgtgc cacaggattt gcttcaagtc
      661 ttttctcgct gatcttctcc tcgcattcct cgcatatgcc gtaggagccc ttgctcagtt
      721 tgtcgaggga gttgtcgatg tccttgagta tggtgagatc gttgtcgctt atgctcatca
      781 ggatgtccgc gttataggtg ttgcttgccg cgtcggccat gtcctggatc ccgtccgtcc
      841 cgaggttata tgaatcctct ttcagcttct tcgccttgtt gagtatgttc tcgcgcatct
      901 tgagaagtgt cttgcggtag aattcaactt tttccttatt cattccccac ctctgtgttc
      961 ttattttcgt tcttgatcct atgccttttc gcttcgatcc acaggctttc cagatcgtag
     1021 agctccctga gctgttcgag aaagatgtgg acgacgacgc tctgataatc gaggatgacc
     1081 cagcggcctt cgtcgtaccc ttcgacggcg agcggcctcg ctccctcctg tttcatgcct
     1141 tcccgtatcc cgtccgcgat ggtcctcaca tgtcgttcgc tggtcccccc ggctataacg
     1201 aagtgatcag cgatgtccgt gatacctgtc aggtccataa cgacaacgtc aagggccttc
     1261 ttgtcgtctg ccagccttcc gcacgcttct accatatcac cgggtttcat ctgaggacct
     1321 gtacaacccc ctttcaatta ttatcttctc cacgccgggt ggcacgagat acctgatgga
     1381 ccgttcgccc ctgaggagat ccctaacctt cgtcgaggag atatccatct gtgtcaccgc
     1441 tcgaaggtat atcttcttgc ccgattcgtg gcggtaaaca ccatctccga gggcggtcag
     1501 tttcacgcgc accgcctcgg gcaccatttc cggtatatcg gccggtagcg aggcgggcct
     1561 cgtcatcacg atgaaaccgg tatgatagaa gagctcttcg taatggtgcc acgtgtctat
     1621 ctgccggaag gcgtcgacgc cgatgatgaa atggatgtca tcgaaacgcc gctccagtct
     1681 cttcagcgtg tcgatggtgt atgatacccc tccccgccgt atctccatgt cggagacctt
     1741 aaaggaggcg ttgcccctga cggcggcctt cagcatcctc aggcgctcgc cggggagccc
     1801 gcccgcggct tccttgtggg gaggaacatg ggatgggatg aaatagacgc tgtccaggtg
     1861 gaaatcctcc ctgatctcct cggcgacgcg caagtgcccc acgtgcaccg ggttgaaggt
     1921 tccgccaaag acaccagttg ccattgtacc gtccttacgt ccgcaattgc ccatcaccga
     1981 aggcgatgaa tttcgtcact gtgagctcct ctaagcccat gggcccgaag gcatggagcc
     2041 tcgttgtgct gatgcccatc tccgcgccga ggccgagctg gaaaccgtcg ttgagccgtg
     2101 tcgaggcgtt gacgagcacc agcgaggagt tgacctcgcg gaggaacttc caggcccttt
     2161 cgtagttcgc tgttacgatg gcctcggtat ggctcgaccc gtacttcctg atatggtcga
     2221 cggcctcgtc catgtccgcg accaccttga cggccagcgt gaggtcgagg tactccatgg
     2281 gccagtcttc cttcgtggcc ctgccgatat cctttaggat ggccaccgtc ttcgggcaac
     2341 ccttcatcgt gacgccctgc cgggagagct ccctgtgcac cttcgggagg aaggacttcg
     2401 cgaccttctc gtgaacgaga agggtctcga gggcgttgca tgtggccggc ttctggacct
     2461 tggcgttgac ggcgacgtcg acggccatcg tgagatcggc ctcgtcgtcg acgtatatgt
     2521 ggcatacccc cttgtaatgt ttgagaacgg gtatgcggga acgttccact atgttgcgga
     2581 taagggcctc gccccccctg ggtatgacga ggtctatttc ctcctccctt tcgagaaggg
     2641 tgtagatgta gtcgcggtcc gcggtgtcca cgaactggac ggcatcgggg gatatcttcg
     2701 accccttcag agtctccacg atgagccggt agagggccct gttggaatgg tacgcctccg
     2761 accctccctt caggatgacg cagttgccgc tcttgagaca cagcgaaaag gcttctatgg
     2821 tcacgttggg acgggactcg tagatgatga ggatgacgcc gatggggatc ctcattctgc
     2881 ccacgagaag gttattgggt cttttccaga ccttgacgat ctccccgacg gggtccggga
     2941 gggccgccac atctctcaag ctcccctgca tctccctgat gagtttgtca tcgatgcgga
     3001 ggcggtcgac gagactcttt gccatgcctt ccttctcggc ggcgcgcacg tccttcatgt
     3061 tctcttcgta caggtaatcc tggttcttcc cgagcgcctc ggcaag
//
LOCUS       DAOQTR010000246         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_36546_length_3099_cov_3.394218, whole genome shotgun sequence.
ACCESSION   DAOQTR010000246 DAOQTR010000000
VERSION     DAOQTR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36546_length_3099_cov_3.394218"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..550)
                     /locus_tag="PKN85_06710"
     CDS             complement(101..550)
                     /locus_tag="PKN85_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT44135.1"
                     /translation="MNKRIPRIYVFHCATGYDQKELMRGHSRQEDEIRMVSLPCSGKL
                     DILYLTKAFDTGADGAAVMICKEGACRYMEGNMRARKRVEAVEELLEEAGLGKGRTTV
                     IELDDEGIAGAIHKLEDFRLQIRTMVENAAAGGVDSSSSRDGKECRV"
     gene            complement(609..1388)
                     /locus_tag="PKN85_06715"
     CDS             complement(609..1388)
                     /locus_tag="PKN85_06715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HNT44136.1"
                     /translation="MEKKLLIIKQVEQEGPGCIADLFSEDGWTVETLEMSGAGTPLPH
                     DLDDIGGIVILGGPMNVYQEDAYPFLKDEESLIRRALIDEVPLLGICLGAQLIAKTCG
                     AAVMRSPKREIGWFAVTKTSEGLKDSLFRGNPQHMTVFQWHEDTFDLPGDGVLLAKGR
                     VCANQAFRVGHSAYGLQFHIEATQEMVESWMRDEKEIDVKRVLRDGRRIMETFVDQGR
                     RVVTNFRRIVESSLRYRRIIARFVDERRWLDQRQINWWEAV"
     gene            complement(1452..1697)
                     /locus_tag="PKN85_06720"
     CDS             complement(1452..1697)
                     /locus_tag="PKN85_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44137.1"
                     /translation="MARSDSLRHFPIVKVVPVSVLDGERSRVAGTALGGIVSSAIESL
                     EDAWSLWEADHSDDTLKESFQKLHYQLEYLRRYLSGD"
     gene            complement(1964..2305)
                     /locus_tag="PKN85_06725"
     CDS             complement(1964..2305)
                     /locus_tag="PKN85_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNT44138.1"
                     /translation="MKLIIAIIQPDRLESVKQELYKEDVNLITVSEVLGHGRQMGVTE
                     VYRGVKEMGNLLRKIRVEIAVNEDFAERTVKAIVKGAQTGETGDGKIFVLDLVECIRI
                     RTEERGGEAIG"
     gene            complement(2318..>3099)
                     /locus_tag="PKN85_06730"
     CDS             complement(2318..>3099)
                     /locus_tag="PKN85_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNT44139.1"
                     /translation="GWLREMGALDFAGGTVVHINAGIAAFMTAIFIGKRKGSNGHAIL
                     PHNLPFTILGTALLWFGWFGFNAGSSLGANDIAVNAFVVTNTAAAAAGMTWALIDWIF
                     NKKPTMLGMATGAVGGLVAITPAAGFVSALSAILIGVFVSIFCYFAVAYIKPKFGYDD
                     ALDVFGVHCVGGIWGALATGLFASKAVNPAGADGLFFGNPKQFLIQVIATAVTVAYSF
                     VASFIIYKVIDLVMKVRVPEKDEIVGLDLTQHHEKAYTILE"
BASE COUNT          608 a          984 c          804 g          703 t
ORIGIN      
        1 ccggcgccca ccgtcagacc ggtaccggcc accggcaaat actcggcggt aaaaatgaac
       61 tccttgccgc ttatcctggc tgccaacttg ctatcaggtc tcataccctg cactccttgc
      121 cgtcacgtga cgaagaactg tcgactcccc cggccgcggc gttctcaacc atcgtcctta
      181 tctgaagtcg gaaatcttct agcttatgga tagcgcccgc tatgccctca tcatccagtt
      241 cgatcaccgt ggtccggccc tttccgagcc ctgcctcctc gagcaactcc tcgacggcct
      301 ctacccgttt cctggcccgc atgttgcctt ccatgtaccg gcacgcgcct tccttgcata
      361 tcatgacggc ggcgccgtcg gcgccggtgt cgaacgcctt ggtcaggtac aggatatcca
      421 gctttcccga acagggcagc gagaccatcc tgatctcgtc ttcctgccgg gaatgaccgc
      481 gcatcaattc cttctggtca tagcccgtcg cacagtggaa gacatatatc ctgggaatcc
      541 gcttattcaa tgttcacacc ctcacctgcc agatgacaac tcatggcgat ctgtttcgcg
      601 gtcacgtgtc agaccgcctc ccaccagttg atctgtcttt ggtccagcca ccttctctcg
      661 tcgacgaacc gcgctatgat cctgcggtat cgcagggaag actcgacgat cctccggaag
      721 ttggtgacga cccttctccc ctgatcgaca aaggtctcca tgatccttct gccgtcgcgg
      781 aggaccctct tcacatcgat ctccttttca tccctcatcc atgactccac catctcctgc
      841 gtcgcttcaa tatggaactg gaggccgtag gcactgtgcc ctaccctgaa ggcctgattg
      901 gcacagaccc ggcccttcgc gagaaggaca ccgtcgccgg gaaggtcgaa ggtgtcctcg
      961 tgccactgga aaacggtcat atgctgggga ttgcctctga agaggctgtc cttcaagccc
     1021 tcggacgtct tcgtgacggc gaaccacccg atctccctct tcggcgatct cattacggcg
     1081 gcgccgcagg tcttcgcgat aagctgcgcg ccgaggcata taccgagaag cggcacttca
     1141 tcgatcaggg cacgcctgat aagggactct tcgtccttga ggaagggata ggcgtcctcc
     1201 tggtaaacgt tcatgggccc gcccaggatg actatcccgc cgatatcgtc aaggtcatgg
     1261 ggaagcggtg tacccgcgcc ggacatctcg agggtctcca cggtccaccc gtcctcggaa
     1321 aagaggtccg ctatgcatcc cggaccttcc tgttcgacct gttttattat aaggagcttc
     1381 ttttccatgt catcctgcct caccttcccc gcgcctcacg gcgggagcgc ttgcgaattc
     1441 gatctgaagc ctcagtcacc gctaaggtat ctcctgaggt actcaagctg atagtgaagc
     1501 ttctgaaaac tctctttcag ggtatcgtcg ctgtgatcgg cctcccacag ggaccatgca
     1561 tcctcaagcg attcgatcgc gctcgagact atcccgccaa gggccgtacc ggccacacgt
     1621 gacctttcac cgtcgaggac gctcaccgga acgaccttga cgatggggaa atgtcttaaa
     1681 ctatctgaac gcgccacaat acacctcgca taaggccttt tagggctagt tagtccaaga
     1741 cgatgacttt ttttgaacgg ctttgttcgt ttccttgaaa aacccctcgt aggtcgttga
     1801 gggatctgta cgtcaatgta cgcctacagt ataatgcaac accggccaat ttacaacaaa
     1861 atattcgagg ttaacagaca gatacgggtc atgggtgctg ggttatgggt cattgggaaa
     1921 aactctgccg tttcctatta cccataaccc agcacccatc ttcttaccct atcgcctctc
     1981 ctcctctctc ttccgtcctg atgcgaatgc attccacgag gtcgaggacg aagatcttgc
     2041 cgtcgcccgt ctccccggtc tgggcaccct tgacgatggc ctttaccgtt ctttccgcga
     2101 aatcctcgtt gacggctatc tcgacgcgga tcttcctaag aaggttgccc atttccttaa
     2161 ctcctcgata tacctcggta acgcccatct gtctcccgtg gccgagcacc tcgctcacgg
     2221 tgatgaggtt gacatcctcc ttgtagagct cctgttttac ggattcgagc ctgtccggtt
     2281 gtatgatcgc tattatgagt ttcattgtcc tccctcctta ctccaggatc gtgtatgctt
     2341 tctcgtggtg ctgcgtcagg tcgagaccca cgatctcgtc cttttccggc acccgcactt
     2401 tcatgacaag gtcgatgacc ttgtagatga tgaagctggc gacgaagctg taggcgacgg
     2461 tcacggcggt ggcgatcacc tgaatgagga actgtttcgg gttgccgaag aaaaggccgt
     2521 cggccccggc cgggttcacc gccttcgacg cgaaaagccc ggtggccagc gctccccaga
     2581 taccgcccac gcagtggacg ccgaaaacgt cgagggcgtc gtcatacccg aatttcggct
     2641 tgatgtaagc gacggcgaaa tagcagaaga tgctgacgaa gacgccgatg aggatggccg
     2701 acagggcgct gacgaaaccc gccgccgggg tgatcgccac caggccgccc acggcccccg
     2761 tcgccatgcc gagcatggtc ggctttttgt tgaatatcca gtcgatgagc gcccaggtca
     2821 ttcccgcggc ggcggcggcc gtgttcgtga ccacgaaagc gttcacggcg atgtcgttcg
     2881 caccgaggga actgccggcg ttgaaaccga accatccgaa ccacagaagg gccgtcccga
     2941 ggatggtgaa gggcaggttg tggggaagga tcgcgtggcc gttcgaccct ttgcgcttgc
     3001 cgatgaagat cgccgtcatg aaggcggcta tgccggcgtt gatatgcaca accgttcctc
     3061 cggcgaaatc gagggcaccc atctccctga gccaccccc
//
LOCUS       DAOQTR010000247         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37036_length_3072_cov_3.934703, whole genome shotgun sequence.
ACCESSION   DAOQTR010000247 DAOQTR010000000
VERSION     DAOQTR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37036_length_3072_cov_3.934703"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKN85_06735"
     CDS             complement(<1..262)
                     /locus_tag="PKN85_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNT44140.1"
                     /translation="MEGQSSLFNETDFLNYRIVRSKMRKRTMTLKVERNGAVVILVPE
                     RTPDEEISRFFRSKVPWIARKLEEYKDVFGETGPRRYVTGERF"
     gene            complement(308..1690)
                     /locus_tag="PKN85_06740"
     CDS             complement(308..1690)
                     /locus_tag="PKN85_06740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT44141.1"
                     /translation="MKIRFLGAARKVTGSCFHLLTDGTQVLVDCGMNQGRNSDALNRE
                     PFRFHPDQIDTLLLTHAHLDHSGLIPRLVAGGFSGRILTTSATAELAGIILLDSAHIQ
                     EKDAEWQTKKAQRAGKDQVFEPLYTTEDVKACLPQFDQNGYDSLHQLPGGMRFRFVDA
                     GHILGSASLELWFHDGGRDKKIVFSGDVGKSGNPIIRDPQHITEADYVVVESTYGNRF
                     HKDLEASIDELEAAVKETFKRGGNVLLPSFAVGRTQDVLYILNRLVREKRLSDINVYV
                     DSPLADKATKIYMSHPEFFDAETLNTFTFRSSAGMRIHFTTTIEESQKLNRVKSGAII
                     IAGSGMCEGGRIRHHFKHNIWRRECSIIFTGFQVPGTLGRYIVDGAKRAYILGEEMAI
                     RAKVYTIGGFSAHADQGELLEWLGAFTNNPRVFIVHGEEPVALEFEKTVREKLGLTTY
                     VPHPGEELEI"
     gene            complement(1948..2313)
                     /locus_tag="PKN85_06745"
     CDS             complement(1948..2313)
                     /locus_tag="PKN85_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44142.1"
                     /translation="MNDTDIIRDLNTMPVNVKARGLLEMLGEEAREGSLHCVHAALYA
                     IEKGEVVVETDVAETVNAMMNWRPPRLVNFLMLMSGEDYDPPGWETAADLREFAGAIL
                     DDIEAKMVTHFPYYRSPES"
     gene            2425..2580
                     /gene="tatA"
                     /locus_tag="PKN85_06750"
     CDS             2425..2580
                     /gene="tatA"
                     /locus_tag="PKN85_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNT44143.1"
                     /translation="MFGLGVPEIIIILVIVILIFGAGKLPSIMSSLGKGIKDFKKEVK
                     DTDSKDQ"
     gene            2624..>3072
                     /gene="folK"
                     /locus_tag="PKN85_06755"
     CDS             2624..>3072
                     /gene="folK"
                     /locus_tag="PKN85_06755"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNT44144.1"
                     /translation="MDSKIFIGIGSNIGQSFENCKTAIKRISSDKRAGLKSISSFYST
                     SPVSDIRQDDYINCAVEIDWQGSPFDLLDLLNSIESSMGRVREEKNGPRVIDLDILMY
                     GDGIIDNDRLTIPHRELHRRRFAIVPLIEIDPDLIHPALKKPLSGFLT"
BASE COUNT          629 a          912 c          833 g          698 t
ORIGIN      
        1 ggaaccgctc tcccgtcacg tagcgcctgg gccccgtctc gccaaagacg tccttgtatt
       61 cctcgagctt cctcgctatc cagggaactt tcgacctgaa gaaccggctt atctcttcat
      121 cgggcgtcct ttcgggaaca aggatgacga ccgctccgtt cctctccacc ttcagcgtca
      181 tcgttcgttt gcgcatcttg ctgcggacga tgcggtagtt gagaaagtcc gtttcgttaa
      241 agagagaaga ctgcccttcc acgttccctc tgacgtcttg cagctttccc ggaccagtcc
      301 gggcttttca gatctccagt tcctctcccg ggtggggcac atacgtggtg agccccagtt
      361 tttccctcac cgtcttttcg aactccaggg caacaggttc ctcgccgtgg acgataaaga
      421 cccgcgggtt gttcgtgaag gcaccgagcc actccaggag ctccccctgg tcggcatggg
      481 ccgagaaccc gccgatggtg tacacctttg cccgaatggc catctcctct ccgaggatat
      541 aggccctctt cgcgccgtcg acgatgtatc ggccgagtgt tccgggcacc tggaaaccgg
      601 tgaagatgat gctgcactcc cgcctccaga tgttgtgctt gaagtgatgg cggatcctgc
      661 ccccctcgca catcccgctg cccgcgatga tgatggcgcc ggatttgacg cggttgagct
      721 tctgcgattc ctctatggtc gtggtgaagt ggatcctcat ccccgccgaa ctcctgaatg
      781 tgaaggtgtt caatgtttcg gcatcgaaga actccggatg agacatgtag atcttcgtgg
      841 ccttatcggc aagggggctg tccacgtaga cgttgatgtc gctgagacgc ttttccctca
      901 ccagcctgtt gaggatatag agaacgtcct gtgtcctgcc cacggcaaag gacggcagaa
      961 gcacgttccc tccccgcttg aaggtctcct tgacggccgc ttccagttcg tcgatgctcg
     1021 cttccagatc tttgtgaaag cggttcccgt acgtggattc aaccacaaca taatcggcct
     1081 cggtgatgtg ctgagggtcc ctgattatcg ggttcccgct cttgccgaca tccccggaga
     1141 aaacgatctt cttgtcccgg cctccgtcat ggaaccacag ctcgaggctg gccgatccca
     1201 ggatatgccc cgcgtcgacg aaacggaacc tcatcccgcc gggcagctga tgcagggaat
     1261 catagccgtt ctggtcaaac tgcgggaggc aggccttgac gtcctccgtc gtgtaaaggg
     1321 gctcgaacac ctggtcctta ccggcccttt gcgccttctt cgtctgccac tcggcatcct
     1381 tttcctggat atgcgccgaa tcgagaagga tgatcccggc cagttccgcc gtcgcggacg
     1441 tggtcagtat cctgccggaa aagccgccag ccaccagacg cggtatgagt cccgaatggt
     1501 cgaggtgcgc atgcgtgaga aggagcgtgt cgatctggtc gggatggaac cggaaaggct
     1561 cccggttgag ggcgtcggaa ttcctgccct ggttcatacc gcagtccaca aggacctggg
     1621 taccatccgt caacagatgg aagcacgacc ccgtgacctt tcttgcagca ccgagaaacc
     1681 tgatcttcat tcgtcacccc gcgggaatca gtatggaacc gagcctgatc taagcctgga
     1741 atcacttgat tatacccgga ggattttgtc ttttcaaggg attaaagacg gcttgaatcg
     1801 cttgaatggc ttgaatggct tgagagtgaa aagattgaag gcgattggat cgataaaggc
     1861 gttggtcccg gcttccgccg ggtggacggg ccgtacggtc atgcaaggtc cttaaccctc
     1921 aagcgattca agcgattcaa gccgtttcta ggattcgggg ctgcggtaat aggggaagtg
     1981 ggtcaccatt ttggcctcga tatcgtcgag gatcgccccg gcgaattccc ggaggtcggc
     2041 ggcggtttcc cagccgggag ggtcgtagtc ctcgccgctc atgagcatca ggaaattgac
     2101 cagccgcggc ggcctccagt tcatcatggc gttgactgtc tccgcaacgt cagtctccac
     2161 gacgacctca cccttctcga tcgcatatag cgcggcatgc acgcaatgga gactcccctc
     2221 ccgtgcctct tcgccgagca tctcgagaag cccgcgagcc ttcacgttca cgggcattgt
     2281 gttgaggtcc ctgatgatat ccgtgtcgtt catgtgcacc cgcttctatt tacacctacg
     2341 gcacggatat aatcaaggat tttcacgtcc gaaaccctgt gaaaaagcat tgactcgcct
     2401 tcttttccga tgatattatt agtaatgttt ggtttgggcg ttcctgagat aataataatc
     2461 ctggtcatcg tgatccttat attcggtgcc ggcaagctgc cttccatcat gtcgtctctg
     2521 ggcaagggca tcaaggattt caagaaagag gtcaaggaca ccgacagcaa ggatcaatag
     2581 agttgcacgt tggcatcaac tgtgggggca tggaggtacc ggcgtggaca gcaagatctt
     2641 tatcggcatc ggctccaaca tcggccagag cttcgagaac tgcaagacag cgataaaacg
     2701 catatccagc gacaagcggg cggggctcaa gtccatatcg tccttttatt cgacatcacc
     2761 cgtctcggac ataagacagg atgactacat taactgcgcc gtggagatcg actggcaggg
     2821 ctccccgttc gacctcctcg acctcctgaa cagcatcgaa tcctcgatgg gccgcgtgcg
     2881 ggaagagaag aacggaccac gcgtcataga cctcgacatt ctgatgtacg gtgacggcat
     2941 catcgataac gaccgcctca ccatccccca cagggaactt caccgcagaa ggttcgccat
     3001 agtcccctta atagagatag accccgacct tatccacccc gccctcaaga agcccttgag
     3061 cggcttcctc ac
//
LOCUS       DAOQTR010000248         3068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37119_length_3068_cov_3.533355, whole genome shotgun sequence.
ACCESSION   DAOQTR010000248 DAOQTR010000000
VERSION     DAOQTR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37119_length_3068_cov_3.533355"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PKN85_06760"
     CDS             <1..713
                     /locus_tag="PKN85_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNT44145.1"
                     /translation="DRCHGGNESPVIGLPYTTDVMVLKGWIEQAARKGFAVYKVKVSG
                     RKADDRRLIASVASILEALDIRYTLRLDGNQGFTKTACLALADHVTKKKYPVELFEQP
                     LKKDDRRGLREITKRSPLPVILDETVFSAEDMEMALNDGLGHGVNIKTAKSGITESLS
                     IMKLGKQGGLRLMAGCMTETMTGLSAGIAMAMGTAAFDYIDLDSVHFLNHRKQYGEIA
                     VEGAGYRRQEMEDGGKVG"
     gene            826..2151
                     /locus_tag="PKN85_06765"
     CDS             826..2151
                     /locus_tag="PKN85_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNT44146.1"
                     /translation="MTSLNRVSPVERLGRSLRVAGIVLRESLRSFLGNNDFEMSAALA
                     TYSFFALVPLLFLVAAFAADTGGTATWLIAGIEGLIDHLFPRIRDWMSVEFSAAIGHP
                     LTLGGAAAVVVFIAAMSLVDSLRTAFLKIFRKNPPASLFTVQFGNVKRAAAMLLLFAV
                     LIGAEIAHLAIGNILRDRLLFTSVGTILVSICVAALCMAFFYRVFLPVRLTAFKLTAV
                     CVISAVLIILMREVFAFVLRTNPAYGETFGSLKVLFVMIVWVYYCFLAILFGAELAVN
                     TGKRDALLLKRLFAGNVQRALSKGLLARFIRSSAKNDVIFEEGDPGGAMYYIVSGSVD
                     IMRGGKHILTMHSGDYFGEISMLLGTPRSAAARAADEPTELVAISQGNFDLILRDNAP
                     IVLSLLKELAGRLKDTDESLLGRQRQKTTSRPFAAFAAHSSTRRLKRDR"
     gene            complement(2421..>3068)
                     /gene="xseA"
                     /locus_tag="PKN85_06770"
     CDS             complement(2421..>3068)
                     /gene="xseA"
                     /locus_tag="PKN85_06770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNT44147.1"
                     /translation="RLAAWRDRDGSTLDVVIVGRGGGAPEELACFNDERVARAIFAAP
                     WPVVSAVGHETDFSIADFVADVRAPTPTAAAALVVPDRTELIRYVLGLKDSLCQAMEK
                     ALERARYMLVSNVMDFKDRKDFFTSHRLYLDDLTVGLTHNIQFYMKNARTKFEALSQR
                     IHDLNPTAILARGYSITSRADTGAVVTDSSAVSPGEALSITLHKGGLDVSVVDRK"
BASE COUNT          593 a          965 c          878 g          632 t
ORIGIN      
        1 aggatcgatg ccacggaggc aatgagtcgc ctgtcatcgg ccttccttac acgactgacg
       61 taatggttct caagggatgg atagaacagg ccgcccggaa gggttttgcc gtgtacaagg
      121 tcaaggtgag cggcaggaag gccgatgaca ggcgactcat tgcctccgtg gcatcgatcc
      181 tcgaagctct ggatatccgt tataccctgc ggctcgacgg aaaccagggt ttcacaaaga
      241 ccgcctgcct cgctctcgcc gaccatgtga cgaagaagaa ataccccgtc gagcttttcg
      301 aacagcctct gaaaaaggac gatcgccgcg gcctcaggga gataacgaag cgctctcccc
      361 tccccgtcat tctcgacgag accgtatttt cagccgagga catggaaatg gccctcaacg
      421 acggtctcgg ccacggcgtc aacatcaaga ccgcgaagag cgggatcacg gagtccctgt
      481 ccatcatgaa gttggggaag cagggcggct tgaggctcat ggccggctgc atgacggaaa
      541 cgatgacggg gctttccgcg ggcatcgcca tggcgatggg aacggccgcc ttcgattata
      601 tcgatcttga ctcggtacac ttcctcaacc accggaagca gtacggggag atagcggtgg
      661 aaggggcggg gtacagaagg caggagatgg aggacggggg aaaagttggg tgaggggttg
      721 ataggtccta ttctcctata agtcctatca tctgtcccgc tccccacccc ctgccgcctc
      781 ttccctgttc cctgatcccc gatcatctgc tataattgac catccatgac atcccttaac
      841 agagtctctc ctgtcgaaag gctcggccgt tcgctgcggg tagccgggat cgtcctccgc
      901 gagtccctga ggtcattcct cggcaacaat gacttcgaaa tgtccgcggc ccttgcgaca
      961 tacagttttt tcgcccttgt ccctcttctc ttcctcgtgg ccgctttcgc cgcagacacc
     1021 ggaggcaccg ccacatggct catcgccggc atcgagggtc tcatcgatca cctcttcccc
     1081 cgaatccggg attggatgag cgtcgaattc tcggcggcga tcggacaccc cctgaccctc
     1141 ggcggggccg ccgcggtcgt cgtcttcatt gccgccatgt ccctcgtgga ttccctcagg
     1201 acggcctttc taaaaatctt caggaagaac cccccggcgt cactgttcac ggtccagttc
     1261 ggcaacgtga aaagggcagc ggccatgctc ctcctctttg ccgttctcat tggagcggag
     1321 atcgcccatc tcgccattgg aaacatcctg cgggatcgcc tcctcttcac gagcgtcggg
     1381 accatccttg tttcaatttg tgtcgccgcc ctctgtatgg ccttctttta ccgggtcttc
     1441 ctgcccgtaa ggctcacggc attcaagctc acagctgttt gtgtcatctc ggccgtcctc
     1501 atcatcctca tgagggaggt cttcgccttt gtcctcagga cgaacccggc ttacggggag
     1561 accttcggct ccctgaaggt cctttttgta atgatcgtgt gggtctacta ttgtttcctt
     1621 gccatccttt tcggtgccga gctcgccgtc aacacgggga agcgagacgc ccttctcctg
     1681 aaacgcctct tcgccggcaa tgttcagaga gccctctcca agggactcct tgccaggttc
     1741 atcaggagca gcgccaagaa tgacgtcata ttcgaggagg gggaccctgg cggtgccatg
     1801 tactatatcg tctccggctc cgtggacatc atgcgcggcg gcaaacacat ccttaccatg
     1861 cactccggag actacttcgg tgagatatcc atgctcctcg gcaccccgcg gtcggcagcc
     1921 gcccgcgccg ctgacgagcc cacggaactc gttgccatct cccaagggaa cttcgacctc
     1981 atccttcgcg acaacgctcc catagtcctc tccctcctca aggaattggc cggcagactc
     2041 aaggacaccg acgaatccct cctcggccgg caacgacaaa aaaccacctc acgcccgttc
     2101 gccgctttcg ctgctcactc gagcacgcgg aggttaaaga gagatcgctg aaaaaagcac
     2161 tcccgggaat tctcccaatt cccgggaacc ttcgaccctg ctctgacgag cagcggagaa
     2221 gaagacccga tgacaagcat cgttaacagg cgttacggaa ctgtctttac ctcgcgccgg
     2281 aacggcggga tgcgaatgcg gcctgatccc ggcgggcgat gggatcaggc cgcgccttct
     2341 ctctgtgccc tctccttgcg cttctccgtg tcctcgagtg aggagcgaaa gcgacgaacg
     2401 ggcgtgagac aaggtcttga ttacttcctg tcgaccacgg acacgtcgag gccgcccttg
     2461 tggagcgtga tcgagagggc ctcgcccggc gagacggcgg aggaatccgt gacgacggcg
     2521 ccggtgtccg ccctcgaggt tatggagtaa cccctcgcca ggatcgctgt gggattgagg
     2581 tcgtgtatcc tctggctgag ggcctcgaac ttcgtgcgcg cgttcttcat gtagaactgg
     2641 atgttgtggg tgaggccgac ggtgagatca tcgaggtaga ggcggtggga ggtgaaaaaa
     2701 tccttccggt ccttgaaatc catgacgttg ctgacgagca tgtaccgggc cctttcgagt
     2761 gctttctcca tcgcctggca cagggaatcc ttaagaccca ggacatagcg gataagctcc
     2821 gtcctgtcag ggacaacgag ggcggcggcg gccgtcggag tgggagcacg cacgtcggcc
     2881 acgaagtccg cgatcgagaa gtctgtctcg tgacccactg ccgacaccac cggccagggt
     2941 gcggcaaaga tcgcacgcgc gactcgctcg tcgttgaagc aggccagttc ctcgggtgcc
     3001 ccgccgccac gcccgacgat caccacgtcc agggtgctgc cgtcgcggtc acgccaggct
     3061 gcgagccg
//
LOCUS       DAOQTR010000249         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37286_length_3058_cov_2.959374, whole genome shotgun sequence.
ACCESSION   DAOQTR010000249 DAOQTR010000000
VERSION     DAOQTR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37286_length_3058_cov_2.959374"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PKN85_06775"
     CDS             <1..930
                     /locus_tag="PKN85_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNT44148.1"
                     /translation="NIAIQGMLHPGDHVVSTRLEHNSVLRPLYHLRQKRIIEYDLVPF
                     DGKGFVDPADIIAAIRPDTKLVVVTHASNVLGTVQPIREIGRRCAEHGVPLLVDAAQS
                     AGAVPIDVDGWQVAALAFTGHKSMLAPTGIGGIVLAGQTEDIEPSRFGGTGIDSASPV
                     HTRDFPYRLEAGTLNLLGIIGLSGSLDYLGEAGIEAVHAREMELVTKLRDGLSLITGI
                     DLYCAEDLSDHVALLTANVRGMDPHDVGAILDADFNIAVRTGVHCAPLVHESLDTSPG
                     GSVRFSVGPFNTGSDIDLTLEAMKEIARHGGKG"
     gene            complement(949..2124)
                     /locus_tag="PKN85_06780"
     CDS             complement(949..2124)
                     /locus_tag="PKN85_06780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT44149.1"
                     /translation="MQNRLWSKQFTAVISATLLLAWAFYALMPTLPIYLMKHLKMGQG
                     NIGMIMAAFSITAIIARPLAGFILDNHPRFRVLIISLIVSTAAYGFYPLVTGVAAMLI
                     LRLVHGAIWGTCSSANATVVADIVPPVRLSEGIGIFALCIPVGMTIGPMFGNEVLSGY
                     GPNAMFLSLLAVSLLAVIIALFARTPPRAITRTRFSIRNLFHRKALPLSLTMFFIMIA
                     YGAIIVFVGIYAGQRGFRNVTAFFLCFSASIFFSRLFLGRLFDRGYFHHLVVAGLTLT
                     AAGLPWLGLAATPLQFLGAGMVSGFGFGILMPTCQAAVNDLADVDERGAANSTYLVSY
                     DLGAGVGVLLIGFLAERIPLGRIYACCFFPILLSAGIFTFAALPHYLRNRSGNRGAG"
     gene            complement(2340..2846)
                     /locus_tag="PKN85_06785"
     CDS             complement(2340..2846)
                     /locus_tag="PKN85_06785"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HNT44150.1"
                     /translation="MAIPSLDLMDQEQKNALLELVLWHYKVIDGFWFLHVSDAFGQSS
                     AESVVEKVWEDAARLASREIVKRFDIKEKGLAGFARAIAFFPMAALVGYDISPEDGAV
                     TISVPHCSAQEARLKHGLGEFKCKDMHRRLFEAFAKEIDPAIRTECLFAPPDPHPADM
                     FCKWRFTI"
     gene            complement(2857..>3058)
                     /locus_tag="PKN85_06790"
     CDS             complement(2857..>3058)
                     /locus_tag="PKN85_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44151.1"
                     /translation="ERWGEQVKAVVVLAKDTHVTEKELIEFCAKHLARYKLPKTVDFV
                     DALPKDPVGKIQKKVLREQYAK"
BASE COUNT          649 a          847 c          984 g          578 t
ORIGIN      
        1 aacatagcca ttcagggcat gcttcacccc ggtgaccatg tcgtgtccac gaggctcgag
       61 cacaactccg tccttcggcc cctttaccac ctgcgtcaga agaggatcat cgaatacgat
      121 cttgtcccct tcgatggaaa aggctttgtc gatcccgcgg acatcatagc ggccatccgg
      181 cccgacacga agctcgttgt cgtgacccac gcgtccaacg ttctgggcac cgtccagccg
      241 atccgggaaa tagggcggcg ttgcgccgag cacggtgtgc cgctcctcgt cgatgccgcc
      301 cagagcgccg gcgcggttcc catcgatgtg gacgggtggc aggtcgcggc ccttgccttc
      361 acgggacaca agtccatgct ggctccgacg gggatcggcg gcattgtcct cgccgggcag
      421 acggaggaca tagaaccttc ccgctttggc gggacgggga tcgattcggc gagccccgtc
      481 cacacccgcg actttcccta ccgcctggag gcgggcaccc tcaacctgct cggcatcatc
      541 ggcctttccg ggagccttga ttatcttggg gaggcgggta tcgaggcggt gcacgcccgg
      601 gagatggaac tcgtcacgaa actccgcgac gggttgtctc tgatcacggg aatagacctt
      661 tattgcgccg aagacctttc cgaccacgtc gcgttgctga cggcaaacgt ccggggcatg
      721 gacccgcacg atgtgggggc catcctggac gctgacttca acatcgcggt cagaacggga
      781 gttcattgtg cccctctcgt tcatgagagt ctggacacgt ctccgggcgg cagtgtgcgt
      841 ttcagtgtcg ggcctttcaa taccggcagc gacatagacc tgacgctcga ggccatgaag
      901 gagatcgcga ggcacggtgg aaagggttga tgtgccgccg acggttgatc agccggcgcc
      961 gcggttgccg gatctgttgc ggagataatg gggaagggcc gcgaacgtga atatgcccgc
     1021 cgacaggagg atcgggaaga agcagcacgc gtagatcctg ccaagaggga tcctctccgc
     1081 aagaaagccg ataagaagga caccgactcc cgctccaaga tcgtaagaaa cgagatacgt
     1141 ggaatttgcc gcgccccttt catcgacgtc cgcgagatcg ttgaccgctg cctggcacgt
     1201 cggcatcagt atcccgaaac cgaagccgct caccatgccg gcgcccagga actggagcgg
     1261 ggttgcagcc aggccgagcc acggcagccc ggccgcggtc aatgtgaggc cggcaacgac
     1321 gagatgatga aaataccccc tgtcgaagag tctgccaagg aagagcctgg aaaagaagat
     1381 ggacgcggag aagcacagga aaaaggcggt gacgttcctg aaacccctct gtccggcgta
     1441 gatcccgacg aagacaatta ttgccccgta agcgatcatg atgaagaaca tggtcaggga
     1501 aaggggaagg gccttcctgt ggaataggtt ccgtatggag aatctcgttc tggtgatcgc
     1561 cctggggggc gtccgcgcga acagggcgat gatgacggcc aggagggaga cggccagaag
     1621 tgaaaggaac atggcatttg ggccatagcc actgagcacc tcgttgccga acatagggcc
     1681 gatggtcatg cccacgggaa tgcacagggc aaagatgcct atgccttcac tgagacgtac
     1741 tggaggcacg atatcggcga cgacggtggc gttcgcagag gaacaggtcc cccatatggc
     1801 gccgtggaca agacggagaa tgagcatggc cgcaaccccc gtcaccaatg gatagaagcc
     1861 gtacgcggcc gtcgaaacga tgagggagat gatgaggaca cggaaccggg gatggttgtc
     1921 gagaatgaat cccgcaaggg ggcgggcaat gatggcggtg atggaaaagg cggccattat
     1981 catcccgatg ttcccctggc ccatcttcag gtgtttcatc aggtagatgg gcagagtggg
     2041 catgagcgcg tagaacgccc acgccaggag caatgtcgcc gatatcacgg ccgtgaactg
     2101 tttcgaccag agtctgttct gcaacgggta tcctttagtg agctgctgga gggatgagcc
     2161 ttccttgcgc tttcagtgat gcaggaacct atcacaaaac ttccgggaat acaaaaaaag
     2221 aatgaaacag gtcatgggtg atgggtgaga ggtgatggga aagagatcgg ggtgctgatg
     2281 atgagggccc ggtcccgttc cggaggggca ggaagggggc cgggcttcaa tcagggggtc
     2341 tagatggtga agcgccactt gcagaacatg tccgcggggt gtgggtcggg gggagcgaag
     2401 agacactctg tccttatcgc gggatcgatc tccttcgcga aggcctcgaa gagcctgcgg
     2461 tgcatgtctt tgcatttgaa ttccccgagg ccgtgtttca ggcgggcctc ctgggcgctg
     2521 cagtggggga cgctgatcgt gacggcgccg tcttccgggg atatatcgta tcccacgagc
     2581 gctgccatgg ggaaaaaggc gatagcgcgg gcgaaaccgg cgagcccctt ctcctttatg
     2641 tcaaaacgtt tcactatttc ccgggaggcg aggcgcgctg cgtcctccca caccttttcg
     2701 acgacgcttt cggcggaaga ctggccgaac gcgtcggaca cgtgaaggaa ccagaacccg
     2761 tcgatcacct tgtagtgcca cagtacaagt tcgagaaggg cgttcttttg ctcctgatcc
     2821 atgagatcga gactgggaat ggccataacg cctcctttat ttcgcgtatt gttcccgaag
     2881 gaccttcttc tgtatcttgc cgactgggtc cttgggaagg gcgtcgacga aatccacggt
     2941 cttcggcagt ttgtagcggg ccaggtgctt cgcgcagaat tcgatgagct ccttttctgt
     3001 tacatgggtg tccttcgcga ggacgacgac ggccttgacc tgctcgcccc agcgttcg
//
LOCUS       DAOQTR010000250         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37403_length_3052_cov_3.683350, whole genome shotgun sequence.
ACCESSION   DAOQTR010000250 DAOQTR010000000
VERSION     DAOQTR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37403_length_3052_cov_3.683350"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKN85_06795"
     CDS             <1..760
                     /locus_tag="PKN85_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44152.1"
                     /translation="FTFAGTVGGDFKTVIDMLATDSRAKLLAVPHVLVSDNKEARIQV
                     GQQVPVVSSETFGSGTVAPQRTIQYKDIGIILKVKPRINEGGLVTLDITQEVSSYETI
                     KLFDNEDYIILKKTETTTSLVVQDGNTIIIGGLIRDDISKSRSGIPLLSKIPILGYLF
                     GQTTQSDQRTELIILLTPRVIRTHADANAVTSDYVDSVTRTGKGKVKREELIKIKPVP
                     PPPAAPQDGFPRDGAERKGGAAQDGFQTQPERVE"
     gene            784..2313
                     /gene="gspE"
                     /locus_tag="PKN85_06800"
     CDS             784..2313
                     /gene="gspE"
                     /locus_tag="PKN85_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNT44153.1"
                     /translation="MSKESAIFLDAEDFPKVPLVIDGISTRFIRENRIIPFEFKNDVL
                     KVIMADPDDREVIEALRVAAAGEVIVYTAAREVIDEHLSKFYSQEEQSINRIMEGMDE
                     ESQELFNEPEEEDIGHLKDLASEAPIIKLVNLLITRAVESRASDIHIEPFGDEVKVRY
                     RIDGVLSDVESAPKRLQAAMVSRIKIMAKLNIAERRLPQDGRIRLLVGDREIDLRVST
                     IPILHGESIVMRILDKESIVIDLDALGFMPDTLNNFNRLIKRPNGIILVTGPTGSGKT
                     TTLYGALDKINSPDKKIITVEDPIEYQLKGVNQIQVRPNIGLSFTNTLRHIVRQDPDV
                     VMIGEIRDLETAEIAIQAALTGHLVFSTLHTNDAPSAITRLLDMGVESFLLSSTVRGI
                     LAQRLVRVICLTCKAEDTSTDEAQELEALGIRGDARLYRGRGCEACTYTGFRGRMGIF
                     ELLLVDEVMRRLILRKADEGEIRDAARSGGMKTLLEDGIRKIEAGSTTLDEVFRVTQE
                     D"
     gene            2320..>3052
                     /locus_tag="PKN85_06805"
     CDS             2320..>3052
                     /locus_tag="PKN85_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT44154.1"
                     /translation="MYSYTATTLEGAIVEGVVEASDERTAVEKIRNTGVIPIKVSLPR
                     EGLGKTFAMRSSRGDLLTFTTELSALLGAGLPIDRSLNILSEISEGKQMKEVINSVLK
                     GIREGSSFSEALQKHPAVFSKLYVSMVRAGEAGGVLDIVLEKLNEFLESAKELKDSIV
                     SAMVYPLILTVTGGISIIILLTFVIPRFSAIFKEIGGSLPLSTQVILTLSQALRSWWW
                     VLFPVAAVALVLLRLYAGTERGALRW"
BASE COUNT          700 a          892 c          929 g          531 t
ORIGIN      
        1 gttcaccttc gcgggcaccg tgggggggga tttcaagacc gtcatcgaca tgctcgccac
       61 ggattcgcgc gcgaagctgc ttgccgtccc ccatgtcctg gtgtcggata acaaggaggc
      121 ccgtatccag gtgggtcagc aggtgcccgt cgtctcatcg gagaccttcg gttcgggaac
      181 ggtggcgccc cagcggacga tccagtacaa ggacatcggg atcatcctca aggtgaagcc
      241 ccgcatcaac gagggcggcc tcgtgaccct cgacatcacc caggaggtct cgtcctacga
      301 gacgatcaag ctcttcgaca acgaggacta catcatcctg aagaagacgg agacgacgac
      361 gagcctcgtc gttcaggacg gcaacaccat catcatcggc ggactgatac gggacgacat
      421 ctcgaagtcc cggtccggta tccctctgct cagcaagatc cccatcctcg ggtatctctt
      481 cggccagacc acacagagcg accagaggac ggaactcatc atcctgctca cgccccgcgt
      541 gatccgcacc cacgccgacg ccaacgcggt cacctccgat tacgtggaca gcgttaccag
      601 gacggggaag ggaaaggtca aaagggaaga gctcatcaag atcaagccgg tgcctccgcc
      661 tccggccgct ccacaggacg gtttcccccg ggacggcgcg gaaaggaagg gcggcgcggc
      721 tcaggacggc ttccaaacgc agccggaaag agtggaatag aaaaaggacc atgaggaaca
      781 ccagtgtcaa aagagagtgc gatcttcctc gatgcagaag atttccccaa ggtacccctc
      841 gtaattgacg ggatatcgac acgtttcatt cgcgagaaca ggatcatccc ctttgagttc
      901 aagaacgacg tcctgaaggt gatcatggcg gatcctgacg atcgtgaggt tatcgaggcc
      961 ctgcgcgttg ccgctgccgg agaggtgatc gtttacaccg ccgccaggga ggtcatcgac
     1021 gagcacctgt ccaagttcta cagccaggag gagcagagca tcaaccggat catggaaggc
     1081 atggacgaag agagccagga gctcttcaac gagccggagg aagaggacat cggccatctc
     1141 aaggacctcg cgtccgaggc gcccatcatc aagcttgtca atctcctcat cacgagggcg
     1201 gtggaaagca gggcgagcga catccacatc gagcctttcg gggacgaggt gaaggtgcgc
     1261 taccgcatag acggggtcct gagcgatgtg gaatcggcgc cgaaacggct ccaggcggcc
     1321 atggtatcga ggatcaagat catggcgaaa ctgaacatcg ccgagcgcag gcttccccag
     1381 gacggccgca tcaggcttct cgtgggggac cgcgagatcg acctcagggt atccacgata
     1441 cccattcttc acggagagag catcgtcatg aggatcctcg acaaggagag catcgtcatc
     1501 gacctcgatg ccctgggttt catgcccgac acactgaata atttcaaccg gctcataaag
     1561 aggcccaacg gaataatact cgtcacgggg ccgacgggca gcggcaagac cacgacgctg
     1621 tatggcgccc ttgacaagat aaactcgccc gacaagaaga tcataaccgt cgaggacccg
     1681 atcgagtacc agttgaaggg tgtcaaccag atacaggtga gaccgaacat cgggctcagc
     1741 ttcaccaaca ccttgaggca catagtgcgg caggacccgg acgtggtcat gatcggagag
     1801 atacgggacc tcgagacggc cgagatcgcc atacaggcag ccctcacggg ccaccttgtc
     1861 ttctcgacgc tccacacaaa cgatgccccc agcgccatca cccggctcct cgacatgggg
     1921 gtggagagct ttctcctgtc ctccaccgta cggggcatcc tagcccagag gctcgtcagg
     1981 gtgatctgcc tcacgtgcaa ggccgaggac acgtcaactg acgaggcgca ggaactggag
     2041 gccctgggca tccgcggcga cgcccgcctc tacaggggca ggggctgcga ggcctgcacg
     2101 tacaccggct ttcgcggaag gatggggatc ttcgagctgc tccttgtcga cgaggtcatg
     2161 cgaaggctta tcctgaggaa ggccgacgag ggggagatca gggacgcggc ccgcagcggc
     2221 gggatgaaga ccctcctcga agatggcata cgaaagatcg aggcgggcag cacgaccctg
     2281 gacgaggtct tcagggtcac ccaggaggat taggtggcca tgtattcata taccgccacg
     2341 accctagagg gtgcgatcgt cgaaggcgtc gtcgaggcgt cggacgaaag gaccgccgtt
     2401 gagaaaataa ggaacaccgg ggtcatacca attaaggtgt cgctaccccg ggagggtctc
     2461 ggaaagacct ttgccatgag atcgtcccgg ggtgacctcc tcacgttcac cacggagctg
     2521 tcggcgctgc tgggtgccgg ccttcccatc gacagaagcc tcaatatact ctcggagata
     2581 tcggaaggga agcaaatgaa ggaggtcatc aattccgtgc tcaaaggtat ccgcgaagga
     2641 agctcctttt ccgaggccct ccagaagcat ccggcggtgt tttcgaaact ttacgtgagc
     2701 atggtgcggg caggggaggc aggaggggtt ctcgacatcg ttctcgaaaa actcaatgag
     2761 ttcctcgagt cggcgaagga actgaaggac agcattgtat ccgcgatggt gtatcccctc
     2821 attttgaccg tgacgggcgg gatatccata atcatcctcc tcaccttcgt cattcccagg
     2881 ttctcggcga tattcaagga gataggcggc agtcttcccc tttcgacaca ggtcatcctg
     2941 accttgagcc aggccctgcg gtcctggtgg tgggtgcttt ttcccgttgc cgccgtggcc
     3001 ctggtgctcc tgagactcta cgcgggaacg gaaagagggg ccttgaggtg gg
//
LOCUS       DAOQTR010000251         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37507_length_3047_cov_3.141043, whole genome shotgun sequence.
ACCESSION   DAOQTR010000251 DAOQTR010000000
VERSION     DAOQTR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37507_length_3047_cov_3.141043"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKN85_06810"
     CDS             complement(<1..243)
                     /locus_tag="PKN85_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44155.1"
                     /translation="MVERKKGLVIAAVVIVALALVPLFTGREYVFSILCMLFLYVTLS
                     QSWNILGGYTGQINVGQAAFFGMGALVARLLWVAGAP"
     gene            complement(245..1120)
                     /locus_tag="PKN85_06815"
     CDS             complement(245..1120)
                     /locus_tag="PKN85_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT44156.1"
                     /translation="MEFAQYITYGVINGSLYALVAIGLALIMGIMGIFNIAQGSLAMI
                     AAYGSVLFFQHMKIDPFVSLALTLPAMFLIGVFMYLTMFMSMNKFDAGQKLRNSMLVS
                     FGLVLIMETLATILWTANERTVTTGYSGTVMALGDLRIPYTGLATIVMAIVVIFGLHS
                     FLKHTTFGKSIWAVSQDHDCAMLMGINVRRTTLAAFGIGVALSAFAGAVVSLHTVSPA
                     VGIDWTNKALILIVLAGVGSINGIFFAGLLLGIVEALGVYLVGVHYTGVVGLLIFIIV
                     LMLRPQGLFVRKAGV"
     gene            complement(1137..1853)
                     /locus_tag="PKN85_06820"
     CDS             complement(1137..1853)
                     /locus_tag="PKN85_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44157.1"
                     /translation="MLEITGIDVFHGDLQAVWDVSLSIEGKGITTLIGSNGSGKSTVL
                     STIAGLLKPVRGSISFDGMRIDTLPAHKIVDAGIAMVPEGRRLFREMTVMDNLEMGAI
                     TKRARKMKNETMAWIFDVFPILKERARQRADTLSGGEQQMLAIGRSLMSQPRLLLVDE
                     LSLGLAPVVVQRISQTLDEINKTKGIGIFLVEQNVVMALELAETGHIIENGRIVGTGK
                     ASELLASDQVRQAYLGMEGE"
     gene            complement(1846..2577)
                     /locus_tag="PKN85_06825"
     CDS             complement(1846..2577)
                     /locus_tag="PKN85_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44158.1"
                     /translation="MDLLKVENLSKSFGALWAVNNVSFSVAEGEIVGLIGPNGSGKST
                     VFHLITGLLKANKGRISYQGKDITRFKPYQVSRSGIARTFQIVRPFPEMTTLKNVMVG
                     RAFGSSSARSMHDTEDDARRLLELTGLADKVSATAGHLGLVYRKKLELARALATKPRL
                     LLLDEVMAGLNPTEMMETVEFLKGLRNTGVTMIVVEHVIKALFSMTDRVVVLNAGEKI
                     AEGTPHEISRNEEVIKVYLGDYEHA"
     gene            complement(2643..>3047)
                     /locus_tag="PKN85_06830"
     CDS             complement(2643..>3047)
                     /locus_tag="PKN85_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNT44159.1"
                     /translation="VGRRRPPHFGMFGLLFMAYLGDDEVERLLGKYPLVATTAKSARD
                     SAEFKQRLKRVREVGYSFDDEGAFEDLSGVAAAIRDGSNAVVGAVGCSFISASVNDET
                     RQAVISAVCEAAARISGSLSNTVLPRAIWGRR"
BASE COUNT          660 a          975 c          753 g          659 t
ORIGIN      
        1 gggtgcgccg gccacccaca gcagcctggc cacgagggcg cccattccga agaacgccgc
       61 ctgccccaca tttatctggc ccgtgtatcc tcccaggatg ttccagctct gcgaaagcgt
      121 cacgtagaga aagagcatgc atagtatgct gaacacatac tccctgcccg taaagagagg
      181 gaccaaagcg agggcgacga tcacaacagc agcaattacc agtccttttt tccgttcaac
      241 cacgtcacac ccccgctttt ctcacgaaga gaccctgcgg cctcagcata agcacaatga
      301 tgaagatcag gagacccacc acacccgtgt agtggacacc gaccaggtag acacccaggg
      361 cttccacgat accgagaagc aaaccggcaa agaagatgcc gtttatgctg ccgacaccgg
      421 ccagcacaat gaggatgagt gccttgttcg tccaatctat cccgacagcc gggctcaccg
      481 tatgaagact gaccaccgcc ccggcaaacg cggaaagtgc gacgcctatc ccaaaggcgg
      541 cgagggtcgt ccttctcacg ttgatcccca tgagcatggc gcagtcatgg tcctgggaca
      601 cagcccatat ggatttaccg aaggtcgtat gcttcaggaa ggagtggagc ccgaaaatga
      661 cgactatggc cataacaatg gtggccagac cggtgtaagg aatccgcaga tcgccgaggg
      721 ccatgacggt gccggagtat cccgtggtga cagtcctctc gtttgccgtc cacagtatgg
      781 tcgcgagggt ctccatgatc aggaccagcc cgaaggaaac gagcatcgaa ttcctcagct
      841 tctgcccggc atcgaatttg ttcatggaca tgaacatggt caggtacatg aaaacgccga
      901 tgagaaacat ggcaggcaac gtaagcgcca gggacacgaa ggggtctatc ttcatgtgct
      961 ggaaaaagag gacgctccca tacgcggcta tcatagccag cgatccctgg gctatgttga
     1021 agatccccat tattcccatg ataagcgcaa gaccgatggc gacaagggcg tacaacgacc
     1081 cattgatgac cccataggtg atgtattgag caaactccat atcttctcct taagccttat
     1141 tcgccttcca ttccgagata ggcctgtctc acctgatcgc tcgcgaggag ttcgcttgct
     1201 tttcctgttc ccacaatgcg gccgttctcg ataatgtggc cggtctcagc cagttccagg
     1261 gccatgacga cattctgttc caccaggaag atgcctatcc ctttcgtctt gtttatctcg
     1321 tccagagtct ggcttatcct ctggaccacc accggcgcaa ggccgagaga cagctcatcc
     1381 acgagcagaa gcctcggctg ggacataagg gacctgccta tcgccagcat ctgctgttca
     1441 ccgccgctca gcgtgtccgc cctctgcctg gccctttcct tcaagatggg gaagacatcg
     1501 aagatccatg ccatggtctc gttcttcatc ttcctggccc ttttcgttat ggctcccatt
     1561 tcgaggttgt ccatcacggt catttcccgg aacagccttc gcccctcagg caccatggca
     1621 atgccggcat cgacgatctt atgcgcgggc agcgtgtcga tgcgcatccc gtcgaaggag
     1681 atgcttcccc tgaccggctt cagcaacccc gctatggttg acagcacggt gctcttcccg
     1741 gacccgttgg agcctatgag ggtggttatc ccctttccct ctatactcaa cgagacgtcc
     1801 catacagcct ggaggtcacc atgaaaaaca tctatccctg taatctcaag catgttcgta
     1861 atccccaagg tagactttga tcacttcttc gttccgggag atctcgtgag gcgtaccctc
     1921 ggcgatcttt tctccagcgt tgagcacgac aaccctgtcc gtcatgctga aaagcgcctt
     1981 tatcacgtgc tccaccacga tcatcgttac tcccgtattc cggagccctt tcagaaattc
     2041 cacggtctcc atcatctcgg tagggttcaa cccggccatc acttcgtcca gcagaaggag
     2101 cctgggtttt gtcgccagcg cccgtgcgag ttccagcttc ttcctgtaga cgagacccag
     2161 gtggcccgcc gtcgcggaca ccttgtccgc gaggccggtc agttcaagca gccgccgggc
     2221 atcgtcctcg gtatcatgca tcgatcgcgc gctggaactg ccgaaagccc tgcctaccat
     2281 gacgttcttc aaggtcgtca tttcgggaaa gggtctgacg atctgaaagg tccgcgctat
     2341 cccggaccgt gatacctggt agggtttgaa acgcgtgatg tccttgccct gatagctgat
     2401 cctgcccttg ttggccttga gcagaccggt aatgagatga aacaccgtcg atttgcccga
     2461 accgttggga ccaataaggc ccactatctc cccttcagcg accgagaagg acacgttgtt
     2521 caccgcccag agggccccga acgattttga tagattctcc accttcaaca gatccatact
     2581 cttctaccta tcctcctgcg gctcccgtcc ctcactcttg cgaccgtccg aatccgaact
     2641 catcatctcc tgccccagat cgctctcggc agaaccgtgt tgctgaggct ccccgatatc
     2701 cttgccgccg cttcacatac ggccgagatc accgcctgtc gtgtttcatc gttcaccgat
     2761 gccgagataa aactgcatcc aacggctccc accaccgcat tgcttccatc cctgatcgct
     2821 gcagcgacac cggagaggtc ctcgaatgct ccctcatcgt cgaatgaata gcccacctcc
     2881 cgcactctct tcagcctttg cttgaattcc gcggaatctc tggctgactt ggcggtcgtc
     2941 gcgacgagcg ggtatttccc cagcagccgc tccacctcat catcaccaag gtacgccatg
     3001 aacagcaggc caaacatgcc gaagtgagga ggcctccgcc tgcccac
//
LOCUS       DAOQTR010000252         3044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37551_length_3044_cov_3.364001, whole genome shotgun sequence.
ACCESSION   DAOQTR010000252 DAOQTR010000000
VERSION     DAOQTR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37551_length_3044_cov_3.364001"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKN85_06835"
     CDS             complement(<1..516)
                     /locus_tag="PKN85_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT44160.1"
                     /translation="MNAPHMLYSRDGNVAVLTINRPEARNAFSPEMIRLWREHLDEAR
                     RDDGVTVIVVTGTGDTFCSGGDIRDMAEGKLRSWDMKRFLWEGVHRIILTLEDLDKPV
                     IAAINGAAMGAGMDMAIMCDLRVCSDRAKLGESYIQMGLIPGDGGAWFLPRLVGVSKA
                     LELLLTADVIDA"
     gene            669..2711
                     /gene="fusA"
                     /locus_tag="PKN85_06840"
     CDS             669..2711
                     /gene="fusA"
                     /locus_tag="PKN85_06840"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT44161.1"
                     /translation="MKKQQTRLSRIRNIGIAAHIDAGKTTVTERILYYTGRTHKIGEV
                     HEGTAVMDYLPQEQERGITITSAVTTLYWEDHEVQLIDTPGHVDFTIEVERSLRVLDG
                     MVAIFCGVGGVEPQTETVWHQADKYRVPRIAFINKLDRVGSDFFRVIDMIVEKFGADP
                     IPLQIPLGREDGFYGVVDLITMQAIAWDDEDLGATYRVISIPAEYKEKAASYREKLFD
                     KISLFDDELMELYLEGREIEPSRIYAALRKGTVGLKCVPVLCGAALRNKGIQPLLDAV
                     IRYLPSPLDVPPVTGENPETRELEERRPSDDEDLAALAFKIVMEEGRKLTYIRIYSGS
                     IKVGGDVYNVNLGKKEKISRIYKMHANHKDRVEEARTGAIVGIVGLKETSTGHTLTQT
                     KPLLLEPIEVYQPVISVAVEAARNADQEKLFLALQRIAQEDPTFVVKTDEDAYQTVVS
                     GMGELHLDVIMHRVREEFGIDLQVGKPQVVYKESIEGTLTREHTFEKVINNTLCKGWV
                     SITVAPNTRGEGNFIISRVGEDNPVYPFVNAAEEGVSEAFNIGVLKGYPLTDVRVEIN
                     DATFNNPEFARLTLKMAAYDALRDACQQAGPVLLVPIMSLTVTVPNEFLGEIIADLNS
                     RKCHIANIMTKDKYTVVDANAPLTNMFGYSTDIRSLSQGRASFTMYFSRYDRIENG"
     gene            2704..>3044
                     /locus_tag="PKN85_06845"
     CDS             2704..>3044
                     /locus_tag="PKN85_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin operon repressor"
                     /protein_id="HNT44162.1"
                     /translation="MDNLSEVLRHLREAGGFISGDHIAGRLGVSRTAVWKYMNQLERF
                     GYDIDKSKGKGYHLVGTPDRLYGWEIDRYRSGGGLGSKIIHKDTLDSTNIFAFRQALA
                     GEAEGTCVVAES"
BASE COUNT          663 a          914 c          899 g          568 t
ORIGIN      
        1 ggcgtcgatc acatcggcgg taagaagaag ctccagcgcc ttcgaaacac cgacaagccg
       61 cggcaggaac catgcgcctc cgtcaccggg gatgagaccc atctgaatgt acgattcgcc
      121 gagcttcgcc ctgtcggagc aaaccctcag gtcgcacatg atggccatgt ccatgccggc
      181 tcccatggcg gcgccgttta tcgcggcgat aacgggcttg tcgaggtctt ccagggtgag
      241 gatgatacgg tgcacccctt cccagagaaa tcgtttcatg tcccaggacc tgagtttccc
      301 ttcggccatg tcgcggatat cgccccccga gcagaaagta tcccccgtgc cggtaacgac
      361 gatgacggtg acgccgtcgt cacgccgcgc ctcgtcgagg tgctcgcgcc acaaccgtat
      421 catctcggga ctgaaagcgt tccgggcttc cggccggttg atggtgagta cggccacgtt
      481 cccgtcccgg gaataaagca tgtgcggtgc attcatcgtt ttcatccttt tcgcggtgag
      541 atatgtccct tgaaggaccc catacagtaa acaccttccg ccgcccaatt tcaagtcgcc
      601 aaaagccctt tgctttcagc cttctttgtt gacttttcgg tcatcttagg tctatatctt
      661 atgcgaaaat gaaaaaacag cagacgcggc tttccaggat caggaacata ggaattgccg
      721 ctcacatcga cgccggaaag acgacggtga cggagcggat tctgtactat accggccgga
      781 cccataagat cggcgaggtc cacgagggca ccgccgtcat ggattacctg ccccaggaac
      841 aggagcgcgg catcaccatc acctcggcgg tgaccaccct ctactgggag gaccacgagg
      901 tccagctcat cgacaccccg ggtcatgtgg actttaccat cgaggtcgag cggtccttga
      961 gggtcctcga cggcatggtg gccatcttct gcggtgtcgg cggcgtggag ccccagacgg
     1021 aaacggtgtg gcaccaggcg gacaagtacc gtgtcccccg gatcgccttt atcaacaagc
     1081 tcgaccgcgt ggggtccgat tttttccgtg tcatcgatat gatcgtcgag aagttcgggg
     1141 ccgacccgat ccctttgcag atcccgctgg gaagggagga cggcttctac ggtgtcgtag
     1201 acctgatcac catgcaggcg atagcctggg atgacgagga cctgggggcg acataccggg
     1261 tgatctccat ccccgcagag tacaaggaaa aggcggcctc gtaccgggag aagcttttcg
     1321 acaagatctc tcttttcgac gatgaactga tggagctcta tctggaaggc agggagatag
     1381 agccgtcccg gatctacgcg gcccttcgca agggcaccgt cggcctcaag tgcgttcccg
     1441 ttctctgcgg cgccgccctg aggaacaagg gtatccagcc cctcctcgac gccgtgatcc
     1501 gttatctgcc gtctccgctg gacgtgcctc ccgttacggg tgagaatccc gagacccgtg
     1561 aactcgagga acgccggccg agcgacgacg aggacctcgc ggccctggcg ttcaagatcg
     1621 tcatggaaga ggggagaaag ctcacctaca tccgcatcta ctcggggtcg ataaaggtgg
     1681 gcggcgacgt ctacaacgtc aacctgggga agaaggagaa gatatcgcgc atctacaaga
     1741 tgcatgccaa ccataaggac cgcgtcgaag aggcgaggac gggggccatc gtgggtatcg
     1801 tggggctcaa ggagacctcg acgggacaca ccctcacgca gacgaagccc ctcctcctcg
     1861 aacccataga ggtctatcag cccgtcattt ccgttgccgt cgaagcggcg aggaacgcgg
     1921 accaggaaaa gcttttcctt gccctccaga ggatagcgca ggaagacccc actttcgtcg
     1981 tcaagaccga cgaggacgcg taccagacgg tcgtgtccgg gatgggcgaa ctccacctcg
     2041 acgtgatcat gcaccgtgtc agggaggagt tcggcatcga cctgcaggtg ggcaagccgc
     2101 aggtggtgta caaggagagc atcgaaggga ccctgacccg cgagcacacc ttcgaaaagg
     2161 tcatcaacaa caccctgtgc aagggctggg tgtccatcac ggtggcccca aacacacggg
     2221 gagagggcaa tttcatcatc tcccgggtgg gcgaggacaa ccccgtctat cccttcgtca
     2281 acgccgcgga agagggcgtt tcggaggctt tcaacatagg cgtgctcaag gggtatcccc
     2341 tcacggacgt gcgtgtcgag atcaacgacg cgaccttcaa caatcccgag tttgcccgcc
     2401 tcacgttgaa gatggcggcc tatgacgccc tgcgcgatgc atgccagcag gcgggccccg
     2461 tgttgctcgt acccatcatg tccctcacgg tgacggtgcc caacgaattt ctcggagaga
     2521 tcatcgcgga cctcaactca cgcaaatgcc atatagcaaa catcatgacc aaggacaagt
     2581 acaccgtcgt cgatgccaac gcccccctta cgaacatgtt cggatactcc acggacatca
     2641 ggtctctgtc ccagggacgg gcttccttta cgatgtactt ttcccgctac gacaggatcg
     2701 aaaatggata acttgagcga agtgctccgg cacctgcgcg aagccggcgg attcatatcg
     2761 ggcgaccaca tagccgggag actgggggta tcgcgcacgg cggtgtggaa gtacatgaac
     2821 cagcttgagc gcttcgggta cgacatcgac aaatccaagg gaaagggcta tcatctcgtc
     2881 ggcacgcccg acaggctcta cgggtgggag atagaccggt acaggtcagg cggcgggctc
     2941 ggctcaaaga tcatccacaa ggacacgctc gattcgacga atatctttgc tttcaggcag
     3001 gccctggccg gcgaagccga ggggacctgt gtggtggccg aatc
//
LOCUS       DAOQTR010000253         3044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37556_length_3044_cov_2.910672, whole genome shotgun sequence.
ACCESSION   DAOQTR010000253 DAOQTR010000000
VERSION     DAOQTR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37556_length_3044_cov_2.910672"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PKN85_06850"
     CDS             complement(<1..1323)
                     /locus_tag="PKN85_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNT44163.1"
                     /translation="MNQAELIGKIIEISHSNLELTARINAILNIIAQDLGFDEAIVYT
                     FDKDKRLTCRYANQKSMLFRILSRYRCHIGEGIVGSVAQRRSPQFFTFKDISPRFGCL
                     FYPELDDHLERFRTFSFLPLSDDSYLFGVVVLCSLMRDSLQEQEKVLLSVISRELGGI
                     LRAFELILSSKKRISELAMLSELGKILTSNVGPYELLKSIALIIAKSLSASFVSVKLE
                     YPLLKLETQRFTYGMIDPAAKEYVEQLERSAIKLLRPVSLRNGTGEQLDNPPPFSLYS
                     APILSKNRVLGTITLCGEKSDQHGIEENGQYIINTISNYISSGLENTLLNTRLRNVVR
                     ELTDAQKRLIEQEKFRSLGEMTANIAHEIKNPLVIIGGFTKRLAKKMQNDRTENRYID
                     IILNEVTRLETILNEVLHYVKENQPYEEFCRLQDFLEDILYIVSSDPAW"
     gene            complement(1298..2767)
                     /gene="glgA"
                     /locus_tag="PKN85_06855"
     CDS             complement(1298..2767)
                     /gene="glgA"
                     /locus_tag="PKN85_06855"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNT44164.1"
                     /translation="MKILIASPEIFPFVKTGGLADVTGALPKALRALGVDVRVILPKH
                     KGIEDLGFPMKYSNHRISCQVSQSFVDAEIVESEYEGIKAYLVEKDEYYYRDYLYSTP
                     DGDYLDNAERYIFFAKSILETIKATGFVPDVLHCNDWETALSPVFLKTIYQNDPDLCN
                     VATLLTIHNLGYQGIFWHYDMHLLNIGWEYFTPESLEFFGHINFLKGGIIFSDILNTV
                     SKQYSREIQTPEFGCGLDGILTKRRDDLYGIVNGIDYGDWNPGTDTSLPGRYTIENRA
                     NKAMCKKALQQVFDLPADPKAPLLATISRLADQKGFDLISEAMEKIHGLGVQYVILGT
                     GERKYHDLFTTLAKKFPRSFSIKIAYDNKLAHLMEAGADMFLMPSRYEPCGLNQLYSL
                     KYGTVPVVRGVGGLEDTIIDFTDSPESGTGFKFYEYTKEAMLDAIGRALSLYGDKKAW
                     EDLVTRCMKEDFSWEKSAREYAELYRKAIAKHESSGTDR"
     gene            complement(2776..>3044)
                     /locus_tag="PKN85_06860"
     CDS             complement(2776..>3044)
                     /locus_tag="PKN85_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44165.1"
                     /translation="SAIGKDLYSLAEVLSTILRKYVKVLNNPPYNYIIHTAPAANVGM
                     GHYHWHIEIIPRLTKMAGFEWGTGFYINPTPPEEATTYLRETEI"
BASE COUNT          598 a          797 c          917 g          732 t
ORIGIN      
        1 ccaggccggg tcggaagaaa cgatatacag gatgtcctcc aggaagtcct ggagcctgca
       61 gaactcctcg tacggctggt tctccttgac gtaatggagc acttcgttaa ggatggtctc
      121 caggcgcgtg acctcgttga ggatgatatc gatatagcgg ttctccgtgc ggtcgttctg
      181 catcttcttg gccagccgct tggtgaagcc gccgatgatg acgagcgggt tcttgatctc
      241 atgggctata ttggccgtca tttcgcccag gctgcggaac ttctcctgtt ctatgagcct
      301 cttctgggcg tccgtcaact ccctgaccac gtttctcagg cgtgtattga gcaacgtgtt
      361 ctccagtccg ctcgatatat agttcgatat ggtgttgatt atgtactgtc cgttctcttc
      421 gataccgtgc tggtcggact tctcgccgca gagggtgatg gtgcccagga ccctgttctt
      481 cgatagaatg ggtgcagaat aaagggagaa gggcggaggg ttgtcgagtt gctccccggt
      541 cccgtttctc aaagaaacgg gacgcaggag ttttatggcc gaccgttcca gctgctccac
      601 gtactccttc gcggcggggt ctatcatgcc ataggtgaac cgctgcgttt ccagtttgag
      661 aagggggtat tccagcttga cggacacgaa ggaggcggag agcgacttcg cgatgatgag
      721 cgctatgctt tttaagagct cgtagggccc cacgttggat gtcagtatct tgccgagttc
      781 cgacagcatc gccagctcgc tgatgcgttt tttcgatgag aggatcagtt cgaaggccct
      841 gaggataccg ccgagctcgc gggatatgac ggagaggagg accttctcct gttcctgcag
      901 cgaatcgcgc atgagcgaac acaggacgac aaccccgaag aggtagctgt cgtcggacag
      961 gggaaggaag gaaaaggtcc tgaacctttc caggtgatcg tcgagctcgg gatagaaaag
     1021 gcagccgaac ctcggggata tatccttgaa ggtaaagaac tgcggagacc gcctctgcgc
     1081 cacgctgccg acgatccctt caccgatgtg gcagcggtag cggctcaata tcctgaaaag
     1141 catgcttttc tggttggcgt agcggcaggt cagcctcttg tccttgtcga aggtatagac
     1201 aatggcctcg tcgaacccga ggtcctgggc gattatgttg aggatcgcgt tgatccgcgc
     1261 cgtcagctcg aggttcgagt gcgagatctc aattatctta ccgatcagtt ccgcttgatt
     1321 catgcttcgc aatcgctttc ctgtaaagtt ccgcgtactc gcgggcagat ttttcccagg
     1381 aaaagtcttc cttcatgcag cgggtcacga ggtcctccca ggctttcttg tccccgtaca
     1441 gggaaagcgc cctgccgatg gcatcgagca ttgcctcttt cgtgtattcg tagaacttga
     1501 agcccgttcc gctttcggga gagtcggtga agtcgatgat cgtgtcctcc agaccgccca
     1561 cgcctctgac aacggggacg gtgccgtact tcaggctgta aagctggttg aggccgcacg
     1621 gttcgtaccg ggaaggcatg aggaacatgt ccgcccccgc ctccatgagg tgcgccagct
     1681 tgttgtcgta ggctatcttt atggaaaagg atctggggaa cttcttcgca agggtggtga
     1741 agagatcatg atacttccgt tcccccgtgc ccagaatgac gtactgcaca ccgaggccgt
     1801 ggatcttttc catggcctcc gagataaggt caaaaccttt ctggtcggcg agacgcgaaa
     1861 tggtcgccag aagcggggcc ttcgggtcgg caggcagatc gaagacctgc tggagggcct
     1921 tcttgcacat ggccttgttc gcccggttct cgatggtgta ccgcccgggc agggacgtgt
     1981 ccgtcccggg gttccagtcc ccgtaatcga taccattgac gatgccgtag aggtcgtccc
     2041 gccgcttggt gagaattccg tcgaggccgc atccgaactc cggggtctgg atctccctgc
     2101 tgtactgctt gcttacggtg ttcagaatgt cggaaaagat gatgccgccc ttgaggaaat
     2161 tgatatggcc gaagaactcc agggattccg gggtgaaata ctcccagccg atgttgagga
     2221 ggtgcatgtc gtaatgccag aatatcccct ggtagcccag gttatggatg gtcaggaggg
     2281 tggccacgtt gcagaggtcg gggtcgttct ggtagatcgt cttcaggaag accggcgaaa
     2341 gggccgtctc ccagtcgttg cagtggagaa catcggggac gaagcccgtt gccttgatgg
     2401 tctccaggat gctcttggcg aagaaaatgt agcgctcggc gttgtcgagg tagtcgccgt
     2461 cgggggtgct gtacaggtag tcccggtagt agtactcgtc cttctcgaca aggtaggcct
     2521 ttatgccttc atactcgctc tcgacgatct ccgcgtcgac gaaggactgc gacacctggc
     2581 atgatatcct gtgattggag tacttcatgg gaaaaccgag gtcttctatg cccttgtgtt
     2641 tggggagaat gaccctgacg tcaaccccca gggccctcaa ggccttcggc agggccccgg
     2701 tcacgtcggc gagaccgcct gttttcacaa agggaaatat ctcgggagaa gcaatcagta
     2761 tcttcattgt gccccctata tctctgtttc tctcaggtac gtcgtggctt cctcgggggg
     2821 cgttgggttg atgtagaagc ccgttcccca ttcgaagccg gccattttcg taaggcgcgg
     2881 gatgatctct atatgccagt ggtagtgccc catgccgacg ttagcggccg gggccgtgtg
     2941 gataatataa ttgtagggtg gattgttgag caccttgaca tactttctca ggatcgttga
     3001 aaggacctcg gccagcgaat acaagtcctt gcctatggca gagt
//
LOCUS       DAOQTR010000254         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37584_length_3042_cov_3.798795, whole genome shotgun sequence.
ACCESSION   DAOQTR010000254 DAOQTR010000000
VERSION     DAOQTR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37584_length_3042_cov_3.798795"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKN85_06865"
     CDS             <1..741
                     /locus_tag="PKN85_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT44166.1"
                     /translation="ESIASVVLEGGRELLLPHGSAYLREKPLFFPNEPVRARQFFGFP
                     LITEDTTFGVIGFVSLNEAELKESAIGALRNLSSLLSLYFTARWMREHLDRIRDFDPV
                     TGAVQFPVFLDVVETMARKKQRFSVLSVKLGNMILYNRKMGAEGTNDLLRKVCQVIRY
                     CAGGNAFVARKSGGRFFVLLKGGETFETRNMMKLLYHAVGRSLAEQGLSAGAGVVELG
                     TVSFPDDSPDLWELFDRIEEKKIRKFIE"
     gene            754..1785
                     /locus_tag="PKN85_06870"
     CDS             754..1785
                     /locus_tag="PKN85_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNT44167.1"
                     /translation="MGFFKNLFGMFSTHLAMDLGTANTLIYMKGEGIVLNQPSVVAID
                     NNSGKVIAVGKEAKEYIGRTPPNISAIRPLKDGVIADFDVAKAMIKYFLKVVSNERKI
                     SKPRMVVGVPSGITQVEKKAVIDACFQVGIRDVHLIEEPMAASIGAGMPIELPRGNMI
                     IDIGGGTTEVAIISLSAVAYSESLRVAGDELDEAIVRYLQKKHQLAIGVIAAEKIKIE
                     GASAFPIDSLSDSINVVGKDLLTSIPRSLKLSKEEVREAIEEPISAIIDSVRRALEKL
                     PAEFVSDLNETGMVLTGGGALLKGLDQRIENETGINVIVAEDPLVSVTMGCGRALEEL
                     AKYKKVFIN"
     gene            complement(2000..>3042)
                     /locus_tag="PKN85_06875"
     CDS             complement(2000..>3042)
                     /locus_tag="PKN85_06875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNT44168.1"
                     /translation="ISFNFNQFEKNLRSMKKSGVIPVVAPSRDVIQQAEFVAQRILEL
                     SDEGVPLDRISVLYRAHYHSMEVQMELTRRGIPFEIRSGLRFFEQAHIKDVVSFLRVM
                     SNPDDEVSWKRMLRIFPRIGKKTADHIYAYLKEQSDPFDPLISGRIADTFKGIRKENV
                     EVWSELFGELKDLFSDEAPGDMISAVLKHGYIEYLKLTYPNYEARLEDLGQLINFSTR
                     YHSLETFLSELSLMSGISGEEIVAAGDTDDERVVLSTIHQAKGLEWQVVFIIWCAEGR
                     FPNPRAIEEGAIEEERRLFYVASTRAMDELYLSYPILAFDRQAGHIIMRPSRFLAELD
                     GARYEEWAISEY"
BASE COUNT          628 a          844 c          953 g          617 t
ORIGIN      
        1 gagagcatcg cctcggtggt gctcgagggg ggcagggagc ttctcctgcc ccacggtagc
       61 gcgtacctca gggagaagcc gctcttcttt cccaatgaac ccgtgagggc gcggcagttc
      121 ttcggctttc ccctcataac ggaggacacg accttcggcg tcatcggttt tgtgtccctc
      181 aacgaggcgg agctcaagga aagcgccata ggggcgctga gaaacctttc gagccttctt
      241 tcgctgtatt tcaccgcccg gtggatgcgc gagcacctgg acagaatacg cgatttcgat
      301 cccgtcacgg gcgccgtgca atttcccgtc ttcctcgatg tcgtcgagac gatggccaga
      361 aagaaacagc ggttctccgt cctgagcgtg aagctgggga acatgatcct ttacaacagg
      421 aagatggggg ccgaagggac gaacgacctc ctgaggaagg tctgccaggt catccggtac
      481 tgcgccgggg ggaacgcctt cgtggcccgc aagagcggag gccgtttctt tgtccttctc
      541 aagggcggcg agacctttga aacacggaac atgatgaagc tcctctatca cgcggtgggc
      601 aggagccttg ccgagcaggg actctcggcg ggagcgggcg tggtggaact cggaacggtc
      661 agtttccccg atgattcgcc tgacctctgg gagcttttcg acaggataga agagaagaag
      721 atacgaaaat tcatcgaata aggaggaagg gggatgggtt ttttcaagaa tttgttcggg
      781 atgttctcca cgcaccttgc catggacctt ggcaccgcca acaccctgat ttacatgaag
      841 ggggaaggca tcgtcctcaa tcagccttcc gtcgtcgcca tcgataacaa ctccggaaag
      901 gtcatcgccg tcggcaagga ggcgaaggaa tacatcggca ggacccctcc caacatcagc
      961 gccatacggc ccctgaagga cggggtcata gcggacttcg acgtggccaa ggcgatgatc
     1021 aagtacttcc tcaaggtcgt gagcaacgag cggaagatat cgaagccgag gatggttgtc
     1081 ggtgtcccct cgggcatcac ccaggtggag aagaaggccg tcatcgacgc ctgcttccag
     1141 gtgggcatcc gtgacgtcca tctcatagag gagcccatgg cggcatccat tggcgcgggc
     1201 atgcccatag aactgccacg gggcaacatg ataatagata tcggcggcgg cacgacggag
     1261 gttgccatca taagcctctc cgcggtggcc tacagcgaat ctttgcgggt ggccggcgac
     1321 gagctcgacg aggccattgt ccgctatctc cagaaaaagc atcagcttgc cataggggtc
     1381 atcgcggcgg agaagatcaa gatagaaggc gcctccgcgt tccccataga ttccttgagc
     1441 gactcgataa acgtcgtggg gaaggacctg ctcacgagca taccgcggag cctgaaacta
     1501 tcgaaggagg aggtccgcga ggccatcgag gaacccatct cggcgatcat cgactccgtg
     1561 agaagggccc tcgagaaact gcccgccgaa ttcgtctccg acctcaacga gacgggcatg
     1621 gtgcttaccg gcggtggggc tcttctcaag ggactcgacc agcggataga gaacgagacg
     1681 gggataaacg tgatcgtcgc cgaggacccc ctcgtatccg ttaccatggg ctgcggcagg
     1741 gctttggaag agctggcgaa gtacaagaaa gtctttataa actaaccaga agacggcttg
     1801 aaccgctcga accgctcgaa ccgctcgagc gtgaaaggaa acggcagaaa aatggccgga
     1861 atcgccccgt tgccgtcgat ctgtaagtga tcaccgtgtt cgcacctttg ggaggttctt
     1921 ttatccttcc catttctttt ctgcgtatcg ccgtgaatgg ttcgggttgt tctaacgttc
     1981 aagcggttca agcggtcttt caatattccg agattgccca ctcctcgtac ctggccccgt
     2041 cgagctcggc gaggaagcgg gaggggcgca tgatgatgtg accggcctgg cggtcgaagg
     2101 cgaggatggg gtagctgagg tatagctcgt ccatggcgcg cgtcgatgct acgtagaaga
     2161 ggcgcctctc ctcttcgatg gccccttcct ctatcgccct ggggttcggg aaccttcctt
     2221 cggcgcacca gatgatgaag acgacctgcc attcgaggcc cttggcctga tggatggtgc
     2281 tcagtacgac ccgttcatcg tccgtgtcgc cggcggcgac gatctcttcg ccggagatgc
     2341 cgctcatgag ggagagctcg ctgaggaatg tctccaggga gtggtacctc gtcgagaagt
     2401 tgatgagctg ccccaggtct tcgagacgcg cctcgtagtt gggataggtg agcttgaggt
     2461 actcgatgta gccgtgcttg aggacggcgg agatcatgtc ccccggggcc tcgtcgctga
     2521 agaggtcctt cagctcgccg aagagctccg accacacctc gacgttctct ttcctgatgc
     2581 ccttgaaggt gtccgcgatc ctgcccgaga tgaggggatc gaaggggtcg ctttgttcct
     2641 tgagataggc atagatgtgg tcggcggtct tcttgccgat gcggggaaag atcctgagca
     2701 tccgcttcca tgaaacctcg tcgtcggggt tcgacatgac gcgcaggaag gagacgacgt
     2761 ccttgatatg ggcctgctcg aagaacctga gcccgcttcg gatctcgaag gggatgcccc
     2821 ggcgggtgag ctccatctgc acttccatcg aatgatagtg ggcgcggtag aggaccgata
     2881 tcctgtcgag ggggacgccc tcgtcggaga gctccagtat ccgctgggcc acgaactcgg
     2941 cctgctgtat cacgtcccgc gacggcgcga cgacggggat gacgccgctc ttcttcatgc
     3001 tccgcaggtt cttttcgaac tggttgaagt tgaaggagat gg
//
LOCUS       DAOQTR010000255         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37585_length_3042_cov_3.669568, whole genome shotgun sequence.
ACCESSION   DAOQTR010000255 DAOQTR010000000
VERSION     DAOQTR010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37585_length_3042_cov_3.669568"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKN85_06880"
     CDS             <1..230
                     /locus_tag="PKN85_06880"
                     /inference="COORDINATES: protein motif:HMM:NF021941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44169.1"
                     /translation="LKDLIDVAKELGRIEKELARITEDSEKLMKKLGNEAFRQKAPAE
                     VIARNEAQYSELSAKREKLIQSRKVLENLSR"
     gene            234..1535
                     /locus_tag="PKN85_06885"
     CDS             234..1535
                     /locus_tag="PKN85_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HNT44170.1"
                     /translation="MPLNFSLIESFIADHQKDMRNKAMLRHDRYRVLSELGTALQTVL
                     KDYEIFETELNALVIEIIDNTSSYETLRECHERAVAGVENFFLEEDTIVDVHDLLRII
                     RDRIAIRVLELVEKEMIGEGLGKPPVDYVWAGLGSEGRDEQTLVTDQDNLLVYAETDD
                     GFASDELRKRCAAMMTEGPVPPNALDAKTLLDGYFRIFAGKASDRLHQVGFEKCKGGV
                     MPVNDRWRGSIKDWKKRIDERMTFGTGIFEPLDVIILTDARRVNGSQAILGELLDYFF
                     ALLTNNKHFMKEFIQSAVLMPSALTFFGGFKVEKEGENKDKFNIKLLGWSPLILSVRM
                     LSLSNGIYERNTLKRIAILRQRNIIKKEMESSLTDAYFTFVRYRIINQINYRDDEGKI
                     SNFLRPDMLGPDEQERLRRAMKAVEAFQKFIQETLLFGEAV"
     gene            1547..1918
                     /locus_tag="PKN85_06890"
     CDS             1547..1918
                     /locus_tag="PKN85_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT44171.1"
                     /translation="MKVVELMNKDVVTCHPTEKLNIILNKLELFNIAGMPVVDKGKLV
                     GIVCQSDILRGLKNGSMTELSVSDVMTTDVITVPPTESAVLVAKIMVEKHINRVPIVE
                     NDKVVGIVTRGDIIKAVAECG"
     gene            2188..2484
                     /gene="gatC"
                     /locus_tag="PKN85_06895"
     CDS             2188..2484
                     /gene="gatC"
                     /locus_tag="PKN85_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNT44172.1"
                     /translation="MKITRETVEYVAHLARLDLDAGEVDAYTRQIDSILEYMDSLNAL
                     DTEGVEPTSHPVPVGCVMREDAVRDSFTAEESVGNAPARIDTFFKVPPIIEVEE"
     gene            2634..>3042
                     /locus_tag="PKN85_06900"
     CDS             2634..>3042
                     /locus_tag="PKN85_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNT44173.1"
                     /translation="MDILNSTIAQLREMLKKKEISSVELTRYYLDRIGKYNGEILAYL
                     RTTDDYAMKMAREADEKIARGEDAPLLGVPLGMKDILCTKGIETTCASSILKGFVPPY
                     DATVIRKLKEAGFVHLGRLNMDEFAMGSSTENSA"
BASE COUNT          752 a          799 c          926 g          565 t
ORIGIN      
        1 ccctcaagga cctcatcgac gtcgcgaagg aactgggcag gatcgaaaaa gagcttgcca
       61 ggatcaccga agatagtgaa aaattgatga agaagctggg caacgaggcg ttccgccaga
      121 aggcgccggc ggaggtcata gcccgcaacg aggcgcagta cagcgagctt tccgcgaaga
      181 gggagaagct catacaaagc agaaaggtgc ttgagaacct ttcgaggtag ttgatgccct
      241 taaatttttc gctcatcgag tccttcatag cggaccacca gaaggacatg cggaacaagg
      301 ccatgctccg tcatgaccgc taccgtgttc tctcggagct ggggacggcg ctgcagaccg
      361 tcctcaaaga ctacgagata ttcgagaccg agctcaacgc gctcgtcata gagataatcg
      421 acaacacatc gtcctacgaa acccttcggg aatgccatga acgggccgta gccggagtgg
      481 agaacttctt cctcgaagag gacacgatcg tggatgtcca cgatctcctt cgcatcatcc
      541 gggacaggat cgcgatcagg gtcctcgaac tcgtggagaa agaaatgatc ggcgagggcc
      601 tgggcaagcc tccggtggat tatgtctggg cagggctggg aagcgaaggc cgcgacgaac
      661 agacgttggt caccgatcag gacaatctcc ttgtctatgc cgaaaccgat gatggtttcg
      721 cctccgatga actgagaaaa cgctgtgccg caatgatgac ggaaggccct gtcccgccaa
      781 atgccctgga cgccaagact ctcctcgacg ggtatttccg gatattcgcc gggaaggcct
      841 ccgaccggct ccaccaggtc ggattcgaga aatgcaaggg cggcgtcatg cccgtcaacg
      901 accggtggag aggttcgata aaggactgga agaagaggat cgacgaacgc atgaccttcg
      961 gaacggggat cttcgagccc ctcgacgtca tcatcctcac cgacgcccgc cgggtcaacg
     1021 gcagccaggc gatcctcggg gagcttctgg actacttctt cgccctcctc accaataaca
     1081 agcacttcat gaaggagttc atccagtccg ccgtcctgat gccgagcgcg ctcaccttct
     1141 tcggaggttt caaggtcgaa aaggaagggg agaacaagga caagttcaat atcaagctcc
     1201 tcggctggtc gccgctcatc ctctccgtcc ggatgctctc cctgtctaac gggatatacg
     1261 agaggaacac actcaaacgg atcgcgatcc tgaggcagcg caacatcatc aagaaggaga
     1321 tggagagttc ccttacggac gcgtacttca ccttcgtccg ctaccggata atcaaccaga
     1381 tcaattatcg cgacgatgag ggcaagatct cgaacttcct tcggcccgac atgctgggcc
     1441 ccgacgagca ggaaagactg cgcagggcga tgaaggccgt ggaggccttt cagaaattca
     1501 tacaggagac actcctcttc ggagaggcag tataaagggg gttattatga aggtcgtgga
     1561 gctgatgaac aaagacgtgg taacctgtca tccgacggag aaactgaaca tcatactcaa
     1621 caagctggag ctcttcaaca tagcggggat gcccgtcgtg gacaagggaa agctcgtcgg
     1681 catcgtctgc cagagcgaca tcctcagggg gctcaagaac gggagcatga ccgagctttc
     1741 cgtgtccgac gtgatgacga cggatgtcat caccgtgccg cccacggagt cggcggtgct
     1801 cgtggcgaag atcatggtgg agaagcatat caacagggtg cccatcgtcg agaacgacaa
     1861 agtggtcggg atcgtcaccc gcggcgacat catcaaggcc gtggcggagt gcggataaac
     1921 aagcgtctca ggtttcagtc tcaggccttt gaagacactg aagccgggaa cggtatggac
     1981 gacaagacgg tttcaggttc aacgtttcag gttgcaggct gacgcgacgg aacgttgagg
     2041 cgagctacca cgagataccc gggaagtggt ttcagatact tgcgggaata tacttgtaac
     2101 ggtgtggacg gtgacggtct gtcctggtct cccgacccgc gacttgggac ctgcaacccg
     2161 tgacttgaga cttgagaaag gactgacatg aagataacaa gagagaccgt tgagtatgtg
     2221 gcgcatcttg cgaggcttga tctcgacgcc ggggaggtgg atgcctatac gagacagata
     2281 gacagcatcc tcgagtacat ggactcgctg aacgccctcg atacggaggg tgtggagccc
     2341 acgagccatc ccgttccggt gggatgcgtc atgcgggagg acgcggtcag ggactccttt
     2401 accgcggagg aatcggtggg caacgccccg gcaaggatcg acaccttctt caaggtgccg
     2461 ccgatcatag aggtggagga gtaagaaccg gcttgagccg cttgaattgc ttgagccgct
     2521 tgagcgttga gaaacaggaa ggcgctcgaa ttgactcctg cgtcattccg gcgcgggacg
     2581 ggattcggga agaatgtgca gccatgatgt ggttcgtttg gaggagatga ggcgtggata
     2641 tactgaattc gacaatagct cagttgaggg aaatgctgaa gaagaaagag atcagctccg
     2701 tggaactgac gaggtattat cttgaccgca tagggaagta caacggcgag atactggctt
     2761 atctgagaac aacggacgat tacgccatga agatggcgcg cgaggcggat gagaagatcg
     2821 cgcgcggaga agacgcgccc cttctgggcg ttcccctcgg catgaaggat atcctgtgca
     2881 cgaaggggat cgagaccacc tgcgcctcga gtatcctgaa aggcttcgtg cccccctatg
     2941 acgccacggt catcaggaag ctgaaggagg ccggattcgt ccatctcggc aggctcaaca
     3001 tggacgagtt cgccatgggc tcatccaccg agaactcggc ct
//
LOCUS       DAOQTR010000256         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37588_length_3042_cov_3.444258, whole genome shotgun sequence.
ACCESSION   DAOQTR010000256 DAOQTR010000000
VERSION     DAOQTR010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37588_length_3042_cov_3.444258"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1131)
                     /gene="dnaJ"
                     /locus_tag="PKN85_06905"
     CDS             complement(31..1131)
                     /gene="dnaJ"
                     /locus_tag="PKN85_06905"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNT44174.1"
                     /translation="MRSRQHVDYFEVLNVPRGATDDEIKKAYRKLALQYHPDRNPGDH
                     SAEEKFKEINEAYEVLGDEQKRRHYERFGTVGDMGSVFDFGFQGNFDSVFNDLFSDFF
                     GNQRPRQRKGGDLRYNLTIEFEEAVFGGEKEIEIPREERCPVCSGSRVEPGHEPVICK
                     HCGGRGQVRQSHGFFTINRTCEYCGGEGQVIKHPCKNCKGRGTVRTKKTLKVRIPPGV
                     DNNSRLRVRGEGMQQPGDTIPGDLFVVLQVKEHAVFERAGDDIVVQVDVGFPLLCLGG
                     EITIPTIEGESVLKVPAGTQQGKVFRLKGLGVNKSNGYGRGDQLVYLNIVIPSDLTER
                     QKTLVEELAAEFRDSSPQTRKGFKEKFMEFFE"
     gene            complement(1151..1744)
                     /gene="grpE"
                     /locus_tag="PKN85_06910"
     CDS             complement(1151..1744)
                     /gene="grpE"
                     /locus_tag="PKN85_06910"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNT44175.1"
                     /translation="MNEKDKDLLSEEKEVRHEEKGESGQKHEEHKKKKKKEELPSEVK
                     KELEEKDGKIRSLEERLLYLQADFENFKKIKAKEKQDLLKYANEVIIRELLPVIDNLE
                     LALKHAESTEDYKGIHEGVKITLNEFMKVLEKAGVKSIEAVGQKFDPNFHEAFYQEEH
                     EDVEPDTVVSEFQKGYLLNDRLIRPSRVGVSKKPEIQ"
     gene            complement(1753..2811)
                     /gene="hrcA"
                     /locus_tag="PKN85_06915"
     CDS             complement(1753..2811)
                     /gene="hrcA"
                     /locus_tag="PKN85_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNT44176.1"
                     /translation="MYCIRMKGLREILSDRERSVLELIVENYIIFAEPVGSITISRAM
                     KKKVSSATIRNIMGDLEELGFLYKPHAVAGRVPTPRAFRYYVNSLPAPGAPGKKELRA
                     LESLSRLRYAYTEEVMVDASRVLAAISRCPSIVVEPRVDTMLFKEVEFVRLSRNTILI
                     VFVTTSGMVHRRFVDTGEDMEQDVLDEMKGYMNERFSGMPFYALKENIIRDMRRDRED
                     FQRLYRKIQDAVDVLVGEEEKREIYIEGASKMIGIPEFSDVERLKDLFRALENKERLI
                     QLLDRYLSDEGINVILGSESDMKGIEGMSIITSAYRIGEDSYGLLGIIGPMRMNYSRL
                     IPIVDYTARAVTDLFRSM"
BASE COUNT          561 a          942 c          773 g          766 t
ORIGIN      
        1 acctattacc aatgacccgt ccctgtcctt ttattcaaag aattccataa acttttcttt
       61 gaaacccttc cgtgtctgcg gggatgagtc cctgaactcg gcggccagtt cctcgacgag
      121 cgtcttctgc cgctccgtca gatcggaggg aatgacgata ttgagataca cgagctgatc
      181 gcccctgcca tagccgttgg acttgttgac gccgaggccc ttaagcctga acaccttccc
      241 ctgctgtgtg cccgccggta ccttgaggac ggattcgccc tcgatggtgg gtatggtgat
      301 ctccccgccc aggcacagga gcgggaatcc cacatccacc tgaacgacga tgtcatcgcc
      361 ggccctctcg aacacggcgt gttccttgac ctggaggacc acgaagaggt cgcccgggat
      421 ggtgtctccc ggctgctgca taccctcgcc cctcactctg aggcggctgt tgttgtcgac
      481 accggggggt atccggacct tcaacgtctt ctttgtcctg acggtccccc tgcccttgca
      541 gttcttgcag gggtgcttga tgacctgccc ttctcctccg cagtactcgc aggtcctgtt
      601 gatggtgaag aacccgtggc tctgacggac ctgtccgcgc ccaccgcaat gcttgcatat
      661 caccggctcg tggcccggct cgacacgtga accgctgcag acgggacacc tctcttccct
      721 ggggatctcg atctctttct ccccgccgaa caccgcctct tcgaactcta tggtcaggtt
      781 gtacctgaga tcgccccctt tcctctggcg cggccgctgg ttgccgaaga aatcgctgaa
      841 aaggtcgttg aatacggagt cgaaattccc ctggaaaccg aaatcgaaga cggaccccat
      901 gtcgccgacg gtcccgaagc gctcgtagtg gcgccttttc tgctcgtcgc cgaggacctc
      961 gtatgcctcg ttgatctcct tgaacttctc ctcggcgctg tggtccccgg ggttgcggtc
     1021 cgggtggtac tggagggcaa gcttccggta tgccttcttt atctcgtcat ccgtggcccc
     1081 ccgcggaaca ttgaggacct cgaaataatc cacgtgctgc ctgcttctca tggtacgcct
     1141 ttcttacgtc ttactgtatt tcgggtttct tggaaacccc cacccgggaa ggtcttatga
     1201 gtctgtcgtt gaggaggtaa cccttctgaa actccgagac caccgtgtcg ggctccacgt
     1261 cctcgtgttc ctcctggtag aacgcctcat gaaagttggg gtcgaacttc tggcccaccg
     1321 cctcgatgga cttgacgccg gccttctcaa gaaccttcat gaattcgttg agcgttatct
     1381 tcacgccttc atggatgccc ttgtaatcct ccgtggattc ggcgtgcttc agggccagtt
     1441 cgagattgtc gatgacgggg agcagctccc tgataatgac ctcgttcgcg tacttcagga
     1501 ggtcttgttt ctctttcgcc tttattttct taaagttctc aaaatcggcc tggaggtaaa
     1561 gcagcctctc ctcgagggac cgtatcttac cgtccttttc ctccagctcc ttcttcactt
     1621 ccgaaggaag ctcctccttc ttcttctttt tcttgtgctc ctcgtgcttc tgcccgctct
     1681 cgcccttttc ttcgtgtctt acctctttct cctcgctgag caggtctttg tctttctcgt
     1741 tcattgtctt cctcacatcg atctgaaaag atccgtcact gcccgggcag tgtaatcgac
     1801 tatggggata agccgggaat agttcatcct catcgggccg atgatcccga gcaggccata
     1861 gctgtcctca cctatccgat aggccgaggt gatgatgctc atcccttcga tccccttcat
     1921 gtcgctctcg cttccaagga tcacgttgat gccctcgtcg ctcaggtacc ggtcgaggag
     1981 ctgtatcagt ctctccttgt tctcgagggc acggaacagg tccttgagcc tctccacatc
     2041 ggagaactcg ggaataccta tcatcttcga cgccccttct atgtatatct ctcttttctc
     2101 ttcctcgccg accagaacat ctacggcgtc ctgtatcttc cggtagagcc tctggaaatc
     2161 ctccctgtcc ctgcgcatgt ccctgatgat attctccttg agggcataga agggcattcc
     2221 cgaaaaacgc tcgttcatat accctttcat ctcgtcgagc acgtcctgct ccatatcctc
     2281 gcccgtatcg acgaacctcc tgtgcaccat tcccgatgtc gtcacgaaga caatgaggat
     2341 cgtgttgcgc gaaagcctga cgaactccac ttccttgaaa agcatggtgt ccacgcgggg
     2401 ctcaacgacg atgcttggac agcgggaaat ggcagcgaga acgcgggagg cgtcgaccat
     2461 cacctcttcg gtgtacgcat agcgcagccg ggagaggctt tcgagggccc ggagctcttt
     2521 cttgccgggg gccccgggag cgggcaggct gttcacgtag tagcgaaacg ccctgggagt
     2581 gggcacacgc ccggcgacgg catggggctt gtagagaaaa cccagttcct cgagatcgcc
     2641 cattatgttg cggatcgttg ccgagctgac cttcttcttc attgccctgg agatcgtaat
     2701 ggaacctaca ggctcggcga agatgatata attctctacg atgagctcaa gaacagacct
     2761 ctcacgatcg cttagaattt cccgtaaccc tttcattctg atacaataca ttaattatcc
     2821 cgcctgtctt tgtcaagaaa atggcgcggg tcctcgatag ccctcatccg gggggtattc
     2881 agcgagacgt ccccgctttc ttcaggcagg ccgcagaagg accgcatgat catccttagc
     2941 catgaagcag ttgcagctcg acgcaaatga acgatcaaag atgggcagcg gaaccgccaa
     3001 tagccggctg accggcccag cacggcagaa ggaagtcgtg tc
//
LOCUS       DAOQTR010000257         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37614_length_3040_cov_3.684757, whole genome shotgun sequence.
ACCESSION   DAOQTR010000257 DAOQTR010000000
VERSION     DAOQTR010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37614_length_3040_cov_3.684757"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKN85_06920"
     CDS             complement(<1..274)
                     /locus_tag="PKN85_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44177.1"
                     /translation="MSNRKIIIFVSFGCIAASLLLALYFFVSKPERQPVPVAEESNKA
                     VVFKNVKYTGERRGVVDWELTAKVARKYIDRPLIELEDLKGLYKPKP"
     gene            complement(292..1260)
                     /locus_tag="PKN85_06925"
     CDS             complement(292..1260)
                     /locus_tag="PKN85_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNT44178.1"
                     /translation="MDHVRTGKDILRKEAEALLKVMEGLDDSFNRAAETIFGCTGRVV
                     LTGMGKSGLVGKKIAATLSSTGTPALFVHPADSLHGDLGMLQRGDVLIVISNSGETEE
                     VLSIIPWVKRLDIPLVAITGDAASTIARLGDVALVVNVEEACPYNVVPTSSTTATLAL
                     GDALAIAVMERRAFNVEDFASLHPGGSLGRKLFLRVEDLMHTGEAVPGVSAETSMKDA
                     ILEITSKRLGVTGVYDGEGRLAGIITDGDLRRAIERYDDSLLAKSAGEVMTPGPKVVR
                     RDALAAYALKKMEEFSITSLFVVNDEDSARPVGIIHIHDLLRAKVV"
     gene            complement(1248..2069)
                     /gene="kdsA"
                     /locus_tag="PKN85_06930"
     CDS             complement(1248..2069)
                     /gene="kdsA"
                     /locus_tag="PKN85_06930"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNT44179.1"
                     /translation="MQKEITIGNVTVGRRPFVFIGGPCVIEGRDISLRTAESIARITA
                     GLGIPFIFKSSYDKANRTSISGFRGPGLDEGLAILQQVKGEVGVPVLTDVHSAGEAAA
                     AAEVADVLQVPALLSRQTDILIACGRTGRAVNIKKGQFLSPNDMAHAIRKVESTGNRN
                     ILVTERGTSFGYHTLVNDFRAIPIMRDFGYPVVFDATHSVQQPSAMTDRSGGEARFIV
                     PLARAAVAVGVEGVFMEVHPDPARALCDGDNSLPLDELEGVLRTLRRIEEALWTT"
     gene            complement(2054..>3040)
                     /locus_tag="PKN85_06935"
     CDS             complement(2054..>3040)
                     /locus_tag="PKN85_06935"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNT44180.1"
                     /translation="VVEGYEDLVDDLAVIAAKDAEVIYEVLIAFHNEGLDEKITKLLN
                     IWAKKPDLSKWEAIGDTIKHPKKEVDIALVGKYVKLKDSYKSLNEALVHGGIANDCRV
                     NIRYVDSEDIEKGITADLFNVDGVLVPGGFGDRGIEGKINAIEHAREKKIPFLGLCLG
                     MQLAVVEMGRNLADCKDANSCEFNEKTPYPLIYLIEEFTDRENKVQKRDQYSDKGGTM
                     RLGAYPCVLKAGTLAHKAYGQDVIHERHRHRYEFNMKYRERLEKSGMIVSGISPDGTL
                     VEIVEMKGHPWFLGCQFHPEFKSKPFDPHPLFRDFIKASLKRRQERLKCKKK"
BASE COUNT          498 a          941 c          945 g          656 t
ORIGIN      
        1 ccggtttcgg cttgtacagc cccttcaggt cttcgagctc gatgaggggc ctgtctatgt
       61 acttccgggc gaccttcgcc gtgagctccc aatccaccac gccgcggcgt tcacccgtgt
      121 acttcacgtt cttgaagacg acggccttgt tgctttcctc cgctacgggg accggttgtc
      181 tttcaggctt gctcacgaag aaatagaggg caagcaggag gcttgccgcg atgcagccga
      241 aagagacgaa gattatgatc ttcctgttgc tcatgtgtcc tcatatgcca ttcacaccac
      301 cttggccctc aagaggtcgt ggatgtgtat gatgcccacg ggccgcgcgc tgtcctcgtc
      361 gttcacgacg aacagggatg tgatcgagaa ttcctccatt ttcttcagcg cgtaggcggc
      421 gagggcatcc ctgcggacga ccttgggccc cggggtcatg acctcacccg cactcttcgc
      481 caggagagaa tcgtcgtagc gttcgatcgc gcgcctgaga tctccgtccg ttatgatccc
      541 cgcgaggcgc ccttcgccgt cgtagacccc cgtgacgcca agcctcttcg acgttatctc
      601 gaggatggcg tccttcatgg acgtctcagc ggagactccg gggacggcct cgccggtgtg
      661 catgaggtcc tccactctga ggaagagctt cctgcccaga gagccgccgg gatggaggga
      721 cgcgaagtct tccacgttga aggcccgcct ttccatgacg gcaatggcga gggcgtcgcc
      781 gagcgcgagc gtcgccgtgg tgctcgacgt gggcaccacg ttgtagggac aggcctcctc
      841 cacgttcacg acgagggcga cgtcgcccag ccgggcgatg gtggacgcgg cgtctcccgt
      901 tatggcgacg agcgggatgt cgaggcgctt cacccagggt atgatgctga gcacctcctc
      961 ggtctccccg ctgttgctga tcacgatgag cacgtctccg cgctggagca ttcccaggtc
     1021 gccgtggagg gagtcagcgg ggtggacgaa gagtgccggc gtgcccgtgc tcgacaacgt
     1081 ggcggcgatc ttcttgccga cgagtcccga cttgcccatg cccgtcagga cgaccctgcc
     1141 cgtgcacccg aatatggtct ccgccgccct gttaaaggag tcatcgaggc cttccatcac
     1201 cttcaggagg gcctccgcct ccttcctcag gatgtccttt cccgttctca cgtggtccac
     1261 agggcctcct ctatcctcct gagggtccgc aggacgcctt cgagctcgtc gagagggagg
     1321 gaattgtcac cgtcgcagag ggcccgtgcc ggatcgggat ggacctccat gaatacccct
     1381 tccacgccga cggctaccgc cgcccgcgcc aggggaacga tgaagcgggc ctccccgccg
     1441 gaacggtccg tcatggccga gggctgctgc acgctgtgcg tggcatcgaa gaccacggga
     1501 tacccgaagt cccgcatgat gggtatggca cggaaatcat tgacaagggt gtggtacccg
     1561 aaggacgtgc cccgttcggt gaccagtatg ttcctgttcc ccgtggattc gaccttcctg
     1621 atggcgtggg ccatgtcgtt gggagagagg aactgaccct tcttgatatt gacggccctg
     1681 cccgtcctgc cgcaggcaat gagtatgtcc gtctgccggg acaggagcgc cggcacctgg
     1741 aggacgtcgg cgacctcggc cgccgccgcg gcctcccccg cggaatggac atcagtgagg
     1801 acggggacac ccacctcgcc cttgacctgt tggaggatgg caagcccctc gtccagtccc
     1861 ggccccctga agcccgatat ggacgtcctg ttcgccttgt cgtaggagga cttgaatatg
     1921 aagggtatgc caagtccggc cgttatgcgc gcgatgctct cggccgtcct cagggatatg
     1981 tccctgcctt cgatgacaca ggggccgcct atgaagacga aaggcctgcg gccgacggtg
     2041 acgttgccga tggttatttc tttttgcact tcagtctttc ctgccttcgt ttcagcgagg
     2101 ccttgataaa atcccggaag agcggatgcg ggtcaaaggg cttcgatttg aactccggat
     2161 ggaactggca tcccaggaac cagggatggc ccttcatctc gacgatctcg acaagcgtgc
     2221 cgtcagggga gatgcccgac acgatcatgc cgctcttctc caggcgctcg cggtatttca
     2281 tgttgaactc gtaacggtgc cggtgccgtt cgtggatgac gtcctgcccg taggccttgt
     2341 gcgcgagggt gccggccttg aggacgcagg ggtatgcgcc gaggcgcatg gtgccgccct
     2401 tgtcggagta ctggtctcgt ttctgcacct tgttctccct gtccgtgaac tcctcgatga
     2461 gatagatgag gggatagggc gtcttttcgt tgaactcgca gctgttcgcg tccttgcagt
     2521 cggcgaggtt gcgccccatc tccacgacgg cgagctgcat gccgaggcac agaccgagga
     2581 aggggatctt cttttcccgc gcgtgctcga tggcgttgat ctttccctcg atgccgcggt
     2641 ccccgaagcc gccggggacg aggacgccat cgacgttgaa gaggtcggcc gttatgccct
     2701 tttcgatgtc ctcggagtcg acgtagcgga tgttcaccct gcagtcgttc gcgatgcctc
     2761 cgtggacgag ggcctcattg aggctcttgt aggagtcctt gagtttgacg tacttcccga
     2821 cgagggcgat gtccacctct ttcttcgggt gctttatggt gtcgccgatg gcctcccact
     2881 tggaaaggtc cggcttcttc gcccagatgt tgaggagttt cgttatcttc tcgtcgagcc
     2941 cctcgttgtg gaaggctatg aggacctcgt agatgacctc ggcgtccttg gcggcgatga
     3001 cggcgaggtc atctacgagg tcctcatagc cttccacaac
//
LOCUS       DAOQTR010000258         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37698_length_3035_cov_3.635570, whole genome shotgun sequence.
ACCESSION   DAOQTR010000258 DAOQTR010000000
VERSION     DAOQTR010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37698_length_3035_cov_3.635570"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKN85_06940"
     CDS             complement(<1..673)
                     /locus_tag="PKN85_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNT44181.1"
                     /translation="MIDETALAVLRKRYLIRDDAGNPLEDPEGMFRRVAEHVASADGN
                     LDRESRQRVADAYFRVMDDLEFLPNSPTLMNAGRELGQLAACFVLPVEDSLDSIFESI
                     KETARIHQSGGGTGFSFSRLRPAGDVVRSTMGAASGPVSFIKVFNTATDVIKQGGTRR
                     GANMGVLRVDHPDIEEFVTVKNDPAELTNFNLSVAVTDAFMAACREDGAFALVDPRNA
                     HQARKV"
     gene            complement(995..2302)
                     /locus_tag="PKN85_06945"
     CDS             complement(995..2302)
                     /locus_tag="PKN85_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNT44182.1"
                     /translation="MVAKIIRFFQTAIWAMKLEDLPFFKSVMVRTARIVVMTIHKFET
                     DHCQRNASVLTYYSLLNIVPLFAVFFAIAKGFGLEKLMVRQIIQLANDANWQADVTAR
                     IIQFSRSLLSHAKGEVIVGVGIVILLWTVISILGKIEDSFNTIWEARQSRTIIRKFTD
                     YLSMMVLGPILFAVWSSVNVLIVGEVKGFMSDISLIGPITLFLLKLLPYFSIWILLLI
                     LYLVMPNTRVPVRSAVIAAFVSGTLIQIAQWVYIGFQIGVSKQSAIYGSFAAIPLFLA
                     WLQISWIIVLIGAEIANAAENQETYGFHPDYSRVGDARRRLLMLRIFHLLVKRFETGE
                     KPLAAGEIGKTLKIPELFVRKILAELVEVDLVSEITRGARKESRFQPARAIANITIKQ
                     ALDVYEKNDTEEPIREDDGLALSLKALLDTMETSPANVKLKEI"
     gene            complement(2410..2850)
                     /locus_tag="PKN85_06950"
     CDS             complement(2410..2850)
                     /locus_tag="PKN85_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT44183.1"
                     /translation="MRAKTKDRVKVHYKGFLRDGTVFDSSLERGPFVFVIGEGAVIPG
                     FERSIVGMCAGDTKTITLPPEEGYGLHREDLCAVVARSRIPSTINLRQGMKLEVQSSS
                     GSSAQAVVLEVTDSDVTLDLNHPLAGKELVFELQLLEILQSNTQ"
BASE COUNT          653 a          842 c          911 g          629 t
ORIGIN      
        1 ggacctttct cgcctgatgg gcgttgcggg gatcgacgag agcgaatgcc ccgtcctccc
       61 ggcaggcagc catgaaggcg tccgtcacgg ccacggagag gttgaagttg gtgagctccg
      121 cgggatcgtt cttgacggtc acgaactctt cgatatcggg atggtcgacg cggaggaccc
      181 ccatgttggc gccccgcctg gtgccgccct gcttgatgac gtccgtggcg gtgttaaaga
      241 ccttgatgaa cgaaacgggc ccgctggcgg ctcccatcgt cgacctgacg acgtcccccg
      301 cgggcctgag cctggaaaag gaaaaacccg tgcctcctcc gctctggtgg atcctggcag
      361 tttccttgat ggactcgaag atggaatcca gggaatcctc gacgggcaga acgaaacagg
      421 ctgccagctg gcccagctcc cgtccagcgt tcatgagggt gggcgagttg ggcagaaact
      481 ccaggtcgtc cattacccgg aaataggcat cggcgaccct ttgacgggac tccctgtcga
      541 ggttcccgtc ggcggaggca acatgttccg ccacgcggcg gaacatgcct tccggatcct
      601 caaggggatt gccggcatcg tccctgatga gatacctttt cctcagcacc gcaagggcag
      661 tttcgtcgat catcgaggtt cacccttcaa tacctagata ctacgtgccc gccccgtggc
      721 tgtcaatgat agggtagggg tcgacgctaa agcgtctaac gggcacacgg gtctgcgcgc
      781 cggggcgcgc aaacgggtcg gcgcgtaagc gcgcctaacg agtaaaaccc gacaagacag
      841 ggaaagatgg acgacctttc cccgtatgcg tgttaggggt tccggcaccc agctgtccac
      901 gcttttcccc gtgtgctcgt taggcacgaa gtgccgaccc gttaaacgcg tcagcgtcga
      961 ctcgttagcc cgaagggcag acccgtccgc gtctttatat ctccttcagc ttcacgtttg
     1021 ccggggaggt ttccatggtg tcgaggagag ccttgaggga aagggcgagt ccgtcgtctt
     1081 cgcggatggg ctcttcggtg tcgttcttct catagacgtc cagggcctgc ttgatcgtta
     1141 tgttggcgat cgcccgtgcc ggctgaaagc gggattcctt ccgcgccccc ctagttatct
     1201 ccgaaacgag gtcaacttcg acgagctccg caaggatctt cctgacgaaa agctcgggga
     1261 tcttcagcgt cttgccgatc tctccggcag cgaggggctt ctctcccgtt tcaaatcgct
     1321 tcacgaggag atgaaagatc ctcagcatga ggaggcggcg cctggcatca ccgacacggg
     1381 aataatcagg atggaacccg taggtctcct ggttctcggc ggcattggct atctccgccc
     1441 cgataaggac gatgatccag cttatctgca gccacgcgag aaagaggggt atggcggcga
     1501 aactgccata aatggcgctt tgtttcgaca cgcctatctg gaacccgata tagacccact
     1561 gggctatctg tatgagcgtg ccggaaacga aggcggcgat gacggccgac cttacgggta
     1621 ccctcgtgtt gggcatgacg agatacagga tgaggagcag tatccagatg gagaaatagg
     1681 gcaggagctt gagcaggaag agtgtgatgg gaccgataag gcttatgtcg ctcataaaac
     1741 ccttcacctc tccgacgatg agcacgttga cgctgctcca gacggcgaag aggatggggc
     1801 ccaggaccat catggacagg taatctgtga acttgcgtat gatcgtgcga gattgccggg
     1861 cctcccatat ggtgttgaag ctgtcctcga tcttgccgag tatggatatg acggtccaga
     1921 ggaggatgac gatgcccacg ccgacgatca cctcgccctt tgcgtgggag aggagggagc
     1981 gggagaactg gataatcctc gcggtgacgt cggcctgcca gttcgcgtcg ttagcgagct
     2041 ggatgatctg ccggaccatc agtttctcca atccgaaacc cttggcgatc gcaaagaaca
     2101 ccgcgaagag aggcacgatg ttaagcagtg agtagtaagt gaggaccgag gcgttgcgct
     2161 ggcagtgatc ggtctcaaac ttgtggatgg tcatgacgac gatgcgggca gtacggacca
     2221 tgactgactt gaagaaggga aggtcttcga gcttcatcgc ccatatggcc gtctgaaaga
     2281 agcggatgat ctttgctacc attcttattt ctccccctga agtgtttcac atcgtcggat
     2341 aacagtcaag cagaaggggc tcccggagac gcagggaaga agagaagacg gcccgtcacc
     2401 gggaccggat cactgggtat tgctctgcaa tatctccagc agttggagtt cgaatacgag
     2461 ttctttcccc gccaggggat ggttgaggtc gagggtgaca tccgaatcgg ttacctcgag
     2521 aacgacggcc tgggcggagg acccgctcga agactggacc tcgagtttca taccctgccg
     2581 gagattgatc gtggagggga tccgggacct cgccacaacg gcacagagat cttccctgtg
     2641 aaggccatac ccctcttccg ggggaagagt gatggttttc gtgtctccag cgcacatgcc
     2701 cacgatgctt ctctcgaatc cgggtatgac cgcgccttca cctatgacaa agacaaaagg
     2761 cccccgttcg agggaagaat caaaaacggt gccgtccctc agaaaaccct tgtagtggac
     2821 cttcaccctg tctttggtct tagcccgcat agtcaaagcc tccatccctc gtggtcctca
     2881 tcgtgatcag tttagttctt gtaacccgat ttcgcccggt aagtcaagga agacagttga
     2941 aaagacggtt tcaagtttca cgcggaagaa agaaacgcgt taaggaactc tctttcagat
     3001 cacgtactga actcaaaggc cgaagacgct gccct
//
LOCUS       DAOQTR010000259         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37853_length_3027_cov_3.212315, whole genome shotgun sequence.
ACCESSION   DAOQTR010000259 DAOQTR010000000
VERSION     DAOQTR010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37853_length_3027_cov_3.212315"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKN85_06955"
     CDS             complement(<1..878)
                     /locus_tag="PKN85_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNT44184.1"
                     /translation="MDKQTIKVVAREFYKYLDNEKGAAFNTRRAYRGDVEDFVRFVSE
                     SSSDVVDHETIRAYIVHIYKGLKKSSLARKVSAIKMFFRFMKKKGYIDENTSLIIRSP
                     RIEKHLPKFYTIDEMFHFLDFLPRDGWLNMRNRAIFELMYSSGMRASEVLAMDKEDLH
                     LEGMWARVTGKGGKERVLPFGEKAKEALEEYLAAVGIMDKFAANTALFINFRGERLTY
                     RGLLRIMKKHQIKAQLFKNLSLHGIRHSFATHMLDNGADLRGIQELLGHSKLSTTQKY
                     THVSMDKLMEIYDKSHP"
     gene            complement(1441..2772)
                     /gene="der"
                     /locus_tag="PKN85_06960"
     CDS             complement(1441..2772)
                     /gene="der"
                     /locus_tag="PKN85_06960"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNT44185.1"
                     /translation="MMTRKENLISIVGRPNVGKSTFFNRIIGYRKAITEDTPGVTRDR
                     NYGSFEMGGRSFLLVDTGGFSSSEEDGFSKLIMKQIEASLQESAAVIFLLDGKEGAMP
                     ADRDIARMLRRLHKPVYYVVNKMDSKKREETLVDFYELGADTLYPVSAAHGLGIGDLL
                     DDILKGMEEGEGKEPPESVEELHVTRIALVGKPNTGKSSLANRILGSERMIVSEIPGT
                     TRDSVDSRIVRDGRELVLIDTAGLRKKGKISEKVEEYSVSSAVRSIERADVVNLIVDG
                     AEGPGHQEGSIAHLIISRGKGFCIVINKWDLVKKQFAQEKYRDMVYERIPHASFAPVI
                     FSSAIQGTGIDDILDMDFAVYEQLTTRIGTAPLNKAFADFFARHDISYQKGKQVKIFY
                     ASQAKASPPTFVLFSNHPRLVPDHYRKYLENSLRQEFGFMGAPIRIVLKKK"
     gene            complement(2762..>3027)
                     /locus_tag="PKN85_06965"
     CDS             complement(2762..>3027)
                     /locus_tag="PKN85_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44186.1"
                     /translation="LFVVLGIQGMLLVSTFSVTIVMAQKLLPTKLGIASGLMVGFAIG
                     TGGIGVTILGIIADNYGVPVALYSIGVLPFIGFLVSILLRYDD"
BASE COUNT          622 a          860 c          823 g          722 t
ORIGIN      
        1 gggtgggatt tgtcgtatat ctccataagc ttgtccatgg agacgtgagt atatttttgc
       61 gtcgtggaga gcttggaatg cccgagcagc tcctggatcc ccctgaggtc ggccccgttg
      121 tccagcatat gcgtggcaaa ggagtgccta atcccgtgaa gggacaggtt cttgaaaagc
      181 tgagccttga tctggtgctt cttcattatc ctcaaaaggc ccctgtatgt gaggcgctcg
      241 ccccggaaat ttatgaaaag ggccgtgttc gccgcaaact tatccattat ccccactgcg
      301 gccaggtatt cctcgagggc ctctttcgcc ttctcgccga agggcaggac cctttccttg
      361 ccgcctttgc ctgtcacccg cgcccacatg ccctcaaggt ggaggtcttc cttgtccatg
      421 gcgaggacct cgctcgccct catacccgaa gaatacatga gctcgaagat ggccctgttg
      481 cgcatattga gccacccgtc cctgggcagg aaatccagga agtggaacat ctcgtctatg
      541 gtatagaact tggggagatg cttctctatt ctcggactgc ggattatgag ggatgtgttc
      601 tcgtcgatat agcccttctt tttcatgaac ctgaagaaca tcttgatcgc cgagaccttc
      661 cgcgccagcg acgatttctt gaggccttta tatatatgca ctatgtacgc ccggatcgtc
      721 tcatggtcca cgacgtccga ggaactctcc gaaacgaacc gcacgaagtc ctccacatca
      781 ccgcggtacg cccgtctcgt gttgaaggca gcccccttct cgttgtcgag atacttgtag
      841 aattccctcg caacaacctt gatggtctgc ttgtccatag acctattatt gtataatttt
      901 cacgaaaata atcaaggaaa ttcccctgtt gcccgcacgc gccccggggc gggggcgtcg
      961 ctgtctttct cgcccgccgg cagagggctt tcgacatcat tgtcgacacc cgtggcgggc
     1021 ttcgacatat cttgcctgca cggaagaaac cgcatctccc tctcatcgat gtaaggccca
     1081 gatatcatga agatatcaga agaggtcttt catggcacac aagttgctta aaatgattga
     1141 tcacgatggc cgggacttcc ggaatgacag ccaggagatt cagataccct tcccggccat
     1201 cacaacacag caatcccgag gctgttcaag cctcacgagg gctcaggggt taccccttcc
     1261 tcccctgatc cctcaccccc ttataaagac gtttgcaacc gcttgaacag cttgaaccgt
     1321 tcgagggcga ggacatttta cttgcgacgc gtgacgtgcg acgcgatgca cgaaatgctc
     1381 gtcttgcagc gctttccttt cccgttcaag cggctcgaat tgttcaagct gttcaagcgg
     1441 tcatttcttc ttcagcacta tccgtatcgg tgcgcccatg aagccgaatt cctggcggag
     1501 gctgttctcg agatacttgc ggtagtggtc ggggacgagg cgggggtgat tggagaaaag
     1561 gacgaaggtg ggcggtgaag ccttggcctg gctggcgtag aatatcttca cctgtttgcc
     1621 cttctggtag gagatgtcgt ggcgggcgaa gaaatcggcg aaggccttgt tgaggggggc
     1681 ggtgccgatc ctcgttgtca actgttcgta aacggcaaaa tccatgtcaa ggatgtcgtc
     1741 tatcccggtt ccctgtattg ccgaggaaaa gatgacggga gcgaaggagg cgtggggaat
     1801 cctctcgtag accatgtcgc ggtatttctc ctgggcgaac tgcttcttca cgaggtccca
     1861 cttgttgatg acgatgcaaa aacccttgcc cctcgatatg atgaggtgcg ctatgctgcc
     1921 ttcctgatgg ccggggccct ccgccccgtc cacgatgagg tttaccacgt cggcgcgctc
     1981 gatactcctt accgcgctcg acacggagta ttcctccacc ttctcggaga tcttgccttt
     2041 cttcctcaga cccgccgtgt cgatgagaac cagttccctt ccgtcccgga cgatccggga
     2101 atccacggaa tcccgggttg tgccgggaat ctcgctgact atcatcctct ccgagccgag
     2161 aatgcgattg gccagtgagg actttcccgt gttgggcttt cccacgaggg cgatccgggt
     2221 aacgtggagc tcttcgacac tctcgggcgg ctcttttccc tccccttctt ccatcccttt
     2281 caggatatcg tcgaggaggt cgcctatgcc gaggccgtgg gcggcactta cgggatagag
     2341 ggtgtcggcg ccgagttcat agaaatcgac gagggtctcc tcccgcttct tcgagtccat
     2401 cttgttgaca acatagtaga caggtttgtg gagccttctg agcatgcggg cgatgtcgcg
     2461 gtcggcaggc atcgcgcctt ccttgccgtc aagaaggaat atcaccgccg ccgattcctg
     2521 aagggacgcc tcgatctgct tcatgatgag cttcgagaac ccgtcctcct cggatgatga
     2581 gaaacctccc gtgtcaacga ggaggaagct tcgcccgccc atttcgaagg agccgtaatt
     2641 gcggtccctc gtcacgcctg gggtgtcctc cgtgatagcc ttgcggtatc cgatgatcct
     2701 gttgaaaaac gtcgatttgc cgacgttggg tctccccacg atactgataa gattctcttt
     2761 tctagtcatc atacctcaat aaaatgctca ccaggaaccc gatgaagggc aaaaccccta
     2821 tggaatagag tgcgacgggc acgccatagt tgtcggcgat gattcccagt atcgtgacgc
     2881 cgatgccgcc cgttccgatg gcaaagccta ccatgagacc cgacgcaatg ccaagctttg
     2941 tgggaaggag cttctgggcc atgacgatgg tgacggagaa ggtggaaacg aggagcatgc
     3001 cctgaatacc gagaacaacg aagagaa
//
LOCUS       DAOQTR010000260         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37869_length_3026_cov_4.067654, whole genome shotgun sequence.
ACCESSION   DAOQTR010000260 DAOQTR010000000
VERSION     DAOQTR010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37869_length_3026_cov_4.067654"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /gene="secA"
                     /locus_tag="PKN85_06970"
     CDS             complement(<1..2012)
                     /gene="secA"
                     /locus_tag="PKN85_06970"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNT44187.1"
                     /translation="MIGNLIKKIVGTKNERELKRIQPIVDRANELEDSMKALRDDELK
                     AMTPHFRERLAGGEALDSLLPEAFAVAREAAWRTLGMRHFDVQLIGGIVLHEGKIAEM
                     ATGEGKTLVATLPVYLNALTGLGVHVVTVNDYLAKRDAEWMGPVYQFLGLSVGVIVHD
                     LDDAARRHAYGCDVTYGTNNEFGFDYLRDNMHYDLEDCVQREFNYAIVDEVDSILVDE
                     ARTPLIISGPAEESTDKYYKINRLIPQLKRDQDFMIDEKARSAYLTEDGVAKLERVLN
                     IENLYDPKYVDFLHHINQALRAHQLFARDVDYIVKDGQVVIVDEFTGRLMPGRRYSEG
                     LHQALEAKENVKIERENQTLATVTFQNYFRMYKKLAGMTGTADTEAVEFKKIYNLDVV
                     VIPTNRDLIRTNFADVVYRTEKEKFRAAVKEIEELYNAGKPVLVGTLSIEKSERVSEM
                     LKRKGIPHNVLNAKNHESEAEVVAQAGRSKAVTISTNMAGRGTDILLGGNPGFLALAM
                     VKGDKDSAEYAKTLEKAKEICAADKEKVISLGGLHILGTERHESRRIDNQLRGRAGRQ
                     GDPGSSRFYVSLEDEIMRLFGSDRVSPILGKLGMDEDTPIEHPLITKAIENAQTRVEG
                     HNFEIRKYLLEYDNVMNKQRETIYGMRREVMKNGDVREKVMEMVDE"
     gene            complement(2110..2580)
                     /locus_tag="PKN85_06975"
     CDS             complement(2110..2580)
                     /locus_tag="PKN85_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT44188.1"
                     /translation="MISVSVPGWGDLDIEYLVIDYNGTCAFDGKMKESVKEMLERVSR
                     YIKVFIITSDTYGNIDSEGNTIGFSIIKVGKESSSQEKAKIIKELGPEKIVAIGNGSN
                     DVLMLKEASLGIGVIGEEGCSKDVLREADFFVKDINDALSILLHPERIVATLRD"
     gene            2751..>3026
                     /locus_tag="PKN85_06980"
     CDS             2751..>3026
                     /locus_tag="PKN85_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44189.1"
                     /translation="MDQQRKWDVDCVIYDCDGVLFDSFDANRRLYNSIALAARGTGLN
                     DDELCYCHTHTVFQSIAHICRNDAQSERRAREFFASTIDFRDFIPYLT"
BASE COUNT          542 a          886 c          834 g          764 t
ORIGIN      
        1 atctcgtcca ccatctccat gaccttttcc cggacgtccc cgttcttcat gacctcgcgg
       61 cgcatcccgt agatggtctc gcgctgcttg ttcatgacgt tgtcatattc caggaggtac
      121 ttgcgtatct cgaagttgtg gccctcgacc cgcgtctggg cgttctcgat ggccttggtg
      181 atcaggggat gctcgatggg agtgtcttcg tccatgccta gtttgcccag gatgggcgac
      241 accctgtcgg agccgaagag gcgcatgatc tcgtcctcga gggagacata gaagcgtgac
      301 gatcccggat ccccctgcct gccggcgcga ccgcgcagct ggttgtcgat ccgccgcgat
      361 tcgtggcgtt ccgtaccgag gatgtgcagg ccaccgaggg agatgacctt ctccttgtcg
      421 gcggcgcata tctcctttgc cttctcaagg gtcttcgcgt attccgcgga gtctttgtcg
      481 cccttgacca tggcaagcgc gagaaatccg gggttgccgc caaggagtat gtcggtgccg
      541 cggcccgcca tgttcgtcga tatggtgacc gccttcgagc ggcccgcttg tgccaccact
      601 tccgcctcgc tctcgtggtt cttcgcgttg agaacgttat gggggatgcc cttgcgctta
      661 agcatctccg acaccctctc cgacttctcg atggagaggg ttccaacgag gacgggtttt
      721 cccgcgttgt agagctcctc gatctccttc accgcggccc ggaacttctc cttctccgtc
      781 ctgtaaacga cgtcagcgaa gttcgtcctg atgaggtccc tgttggtggg gataacgacg
      841 acgtccaggt tgtagatctt cttgaactcc acggcctccg tgtctgcggt acccgtcatg
      901 ccggcgagct tcttgtacat cctgaagtag ttctgaaagg tgaccgtggc cagggtctgg
      961 ttctccctct ctatcttgac gttctccttg gcctcgagcg cctggtggag accttcgctg
     1021 tagcgccttc cgggcatgag cctgcccgtg aactcgtcga cgatgacgac ctggccgtcc
     1081 ttgacgatgt aatccacgtc gcgggcgaag agctgatggg ccctcaaggc ctgattgatg
     1141 tggtggagaa aatcgacgta tttggggtcg tagaggttct cgatattgag gaccctctcg
     1201 agcttcgcga caccgtcctc ggtgaggtag gcgctgcggg ccttttcgtc tatcatgaag
     1261 tcctggtcgc gtttgagctg gggtatcagc ctgttgatct tatagtactt gtccgtcgat
     1321 tcctctgcgg ggccggagat gatgagcggc gtcctcgcct cgtcgacgag aatgctgtcc
     1381 acctcgtcga cgatggcata gttgaactcg cgctggacgc agtcttccag gtcgtagtgc
     1441 atgttgtcgc ggaggtagtc gaagccgaac tcgttgttcg tcccgtacgt gacgtcgcag
     1501 ccgtaggcgt gcctgcgggc ggcatcgtcg aggtcatgga cgatgacacc cacggaaagg
     1561 cccaggaatt gatacacggg gcccatccat tcggcgtccc tcttcgcgag gtaatcattg
     1621 acggtgacca cgtggacgcc gagacccgtc agggcgttga ggtaaaccgg aagggtggcg
     1681 acgagggtct tgccttcgcc cgtggccatc tcggcgatct ttccctcatg cagcacgatc
     1741 ccgccgatga gctggacgtc gaaatggcgc attcccaggg tgcgccaggc cgcttcgcgg
     1801 gcaacggcaa aggcttcggg caaaagggag tcgagggctt cgcctccggc gagccgttcc
     1861 ctgaaatgcg gcgtcatggc cttaagctcg tcatcgcgta acgccttcat gctgtcttcg
     1921 agctcgttcg ccctgtcgac tatgggctga atgcgcttga gctcgcgttc gttcttggtg
     1981 ccgacgatct tcttgatcag gtttccgatc atggtttcac gtaccttcgg caaaaaaacc
     2041 ttcagctcag gtcatgcttc gcacgtggga aggctgccct gagctgtccg ttcttctccg
     2101 taattcttat cagtctctca atgtcgcgac gatcctctcg ggatggagaa ggatgctcaa
     2161 tgcgtcattt atgtccttca cgaagaaatc ggcctcccgg agcacatcct tcgaacagcc
     2221 ttcctcgccg atcacgccga tcccgaggga tgcttccttg agcatcaata cgtcgttcga
     2281 gccgttgcca atggccacta tcttctcggg ccccagttcc ttgatgatct ttgccttttc
     2341 ctgggagctc gattctttcc ccaccttgat gatgctgaaa ccgatcgtgt ttccttcgct
     2401 gtctatattg ccgtacgtgt cggatgtgat gatgaacacc tttatgtagc gggacaccct
     2461 ttcgagcatc tccttgacgc tttccttcat ctttccgtca aaggcgcagg tcccattata
     2521 gtctatgacc aggtattcga tatcgagatc gccccatccg ggaacggaaa cacttatcat
     2581 aaatagcctc cttggtagtc tttcgcatac aattgtagca tatattaatg caattagaac
     2641 cattaatttg cgtctttaag cgatattctt cacaggtttt ctcttgattt tttgcaactt
     2701 acagtttaaa agcagagggt tcgaaattgc tgcacaagga gaatgggaac atggaccagc
     2761 agaggaaatg ggatgtcgat tgcgtcatat acgactgtga cggagtgctc ttcgactcct
     2821 tcgacgccaa caggcgtctc tacaacagca ttgcccttgc cgcgcgcggt accgggctca
     2881 atgacgacga gctttgctac tgccacaccc acaccgtctt tcaatccatc gcccacatct
     2941 gccgcaacga cgctcagtcc gaacgccgtg cgcgggaatt cttcgcgagc accatagact
     3001 tccgggattt catcccttac ctcacg
//
LOCUS       DAOQTR010000261         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_37945_length_3022_cov_2.852039, whole genome shotgun sequence.
ACCESSION   DAOQTR010000261 DAOQTR010000000
VERSION     DAOQTR010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37945_length_3022_cov_2.852039"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKN85_06985"
     CDS             complement(<1..594)
                     /locus_tag="PKN85_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNT44190.1"
                     /translation="MNGAELVLKTAVLNGIDICFANAGTTEIPLVAALDGQAGIKTVL
                     GLFEGVCTGAADGFGRVLDRPAMALLHLGPGFANGIACLHNARRAKAPVVNVIGEHAS
                     WHRDADPPLCMDIAAMVGAVSGWKRTCRSARGLSRDMAAVIAASSRGQVASLIIPNDH
                     QWAECGGIPIVTASPSFEPVDHEVVDRAAKALKTGGRT"
     gene            1033..2892
                     /locus_tag="PKN85_06990"
     CDS             1033..2892
                     /locus_tag="PKN85_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44191.1"
                     /translation="MSQRQNTREELLAEIAGLRQRIVELETTQTEFSQIEATLRKTEE
                     KYRSIFEDAMEGIFQTSVDGRFLSANPSLSRVHGYDSPQDLIESITNISKQLYVNPED
                     RARTMEKIEREGSVRNFETQMYSKDGSIHWISMNIRAVRDKFNMIRYYEGTMLDITER
                     KKAEAALQESEERYRTAIENSNDGVAIIQGYTLQYVNRRFLEMFEFPSTEEAIGESVL
                     TTIHPDDLSRVRDINQRRQRGEPVPSRYEFKGITRNGRTIYVEVSAATIPYRNATVYL
                     VYLRDVTERKEAEEALIQSRNALESLNRAKTKAVNHISHELKTPLSVIQGSMRLIRRK
                     LEPTAQYVSIRDLVDSIERNLQRLFNISRETDQIFRLSQELEAVMLVGDLDRLWQRME
                     SLSEVPADVRGHWNSLREWIGQYFAASTQFFQSIDLYTFVVSVVERMKARLPGRDLDI
                     RVDGANDLFISMDPQILRDIAEGLIKNAIENTPDGGSIRIGLEQKDDRIVLSVTDTGV
                     GITEENKRFLLDGLHHAKETDLYSSRKPLEFGAGGKGLDLLRIKYYSLRFGFDISFES
                     NRCVHIPTDRDLCPGNISKCPAIRGTEECLASGGTTFTVSFPVRKRSASVDEA"
BASE COUNT          687 a          935 c          835 g          565 t
ORIGIN      
        1 cgttctcccg cccgtcttca aggcctttgc cgcccggtcc acaacctcat ggtcgacagg
       61 ctcgaaggag ggggaggccg tgacgatggg aatacccccg cattcggccc actgatggtc
      121 attggggatg atgaggctcg ccacctgtcc gcgagagctt gccgctatga cagccgccat
      181 gtcgcgggag aggccccggg cgctcctgca cgtcctcttc catcccgaaa cggcccccac
      241 catcgccgct atatccatgc agagcggcgg gtccgcgtca cgatgccagg acgcgtgctc
      301 gccgatgacg ttgacgacag gcgccttcgc gcgccgcgcg ttgtgaaggc aggctatccc
      361 gttggcaaag cccggaccca ggtggagaag ggccatcgcc ggcctgtcga ggacccgccc
      421 aaaaccatcg gccgcgcccg tgcagacccc ctcgaagaga ccgaggacgg tctttatgcc
      481 cgcctgaccg tcaagggccg cgacaagggg tatctccgtg gtcccagcgt tggcgaaaca
      541 gatatcgata ccattcaaca cagccgtctt caacacaagt tcagcgccgt tcatagaccc
      601 cccgatgaaa cagatgatgg tgaaacaggt gataggtcat gggtgatggg aaagccttga
      661 cggccgtacc tattacccat gacctataac ccataacctg ttctattctt ctcagtatat
      721 atggaaccgg gaggatctga caaggcatat cggagtcatg atgaggcagg ttatggtgaa
      781 acaggcaatg ggtaatggga aagccttgaa ggccgtacct attacccatg acccataacc
      841 cataacctgc ttttttgccc ctggcaccca ttttcgtccc ttctatccct gtcgtcttgt
      901 tatccccgtc caaacaactt ataatagagt tgattcagga gacggtaaca ggtaaatagg
      961 tgagaggtga ccgatcatca ccgcctgtcc tttcccgtca cccatgagct gtttcatcga
     1021 gaccgaggtt ccatgtcaca acggcaaaac accagagaag agcttcttgc ggagatcgcg
     1081 ggtctccggc agcgcattgt tgagcttgag acgacccaga cagagttcag ccagatcgaa
     1141 gcgaccctgc gcaagacaga ggagaagtat cgcagcatat tcgaggatgc catggaaggc
     1201 atctttcaga caagcgtgga cggacgattc ttgagcgcca acccttcgct ctcccgcgtt
     1261 cacggatacg attctcccca ggacctgatc gaatccatca cgaacatctc gaagcaactc
     1321 tacgtcaacc ccgaagatcg cgcccggacc atggaaaaaa tagaacggga ggggtcggtg
     1381 cggaacttcg aaacacagat gtacagcaag gacggcagca tccactggat ctccatgaac
     1441 attcgcgccg tccgcgacaa gttcaacatg atccgctact acgaaggcac gatgctggac
     1501 atcacggagc gcaagaaggc ggaggccgcc ctccaggaaa gcgaagagcg ctaccgcacg
     1561 gctatcgaga actccaacga cggtgtggcc atcattcagg gctacaccct ccaatatgtg
     1621 aacaggcgct ttctcgagat gttcgaattc ccttccaccg aagaggccat aggcgagtcg
     1681 gtccttacca ccatccatcc ggacgatctc tcgagagtgc gggacatcaa tcagcgccgc
     1741 cagcgcgggg agccggtgcc gtcgcgatac gagttcaagg gcatcacaag gaatggcagg
     1801 acgatctatg tcgaggtttc agccgccacc attccctatc gcaatgccac cgtctacctc
     1861 gtctatctcc gggatgtgac ggagcgcaag gaggccgagg aggccctcat ccagtcccgc
     1921 aacgccctgg agagcctgaa ccgcgccaag acgaaggcgg tcaatcacat ctcccacgaa
     1981 ctgaagacgc ccctgtccgt cattcagggg agcatgaggc ttataagacg aaaactcgaa
     2041 cccacagccc agtacgtctc catacgggac ctggtcgact ccatcgagcg caatctccag
     2101 agactcttca acatctcccg cgagacggac cagatattca ggctttccca ggaactcgag
     2161 gcggtcatgc tcgttggtga cctcgaccgg ctgtggcaaa ggatggagag cctctccgag
     2221 gtgcctgccg acgtgcgcgg ccactggaac tccctgagag aatggatcgg ccagtatttc
     2281 gcggctagca cccagttctt ccagtccatc gacctttaca cctttgtcgt ttccgttgtt
     2341 gagcgtatga aagcccggct gcccgggcga gatctcgaca tcagggtgga cggggcgaat
     2401 gacctcttta tctccatgga cccccagatc ctccgggaca tcgccgaagg cctcatcaag
     2461 aacgccatcg agaacacgcc tgacgggggt agcataagga tcggcctcga gcagaaggat
     2521 gaccggatcg tattgagtgt gacggacacc ggcgtcggga tcaccgaaga gaacaaacgc
     2581 ttcctcctcg atggtttgca ccacgcgaag gagacggacc tctactcctc aaggaaaccg
     2641 ctggaattcg gcgcgggtgg gaaagggctc gacctcctga ggatcaagta ctactccctg
     2701 cggttcggct tcgacatctc cttcgaaagc aaccgctgcg tgcatatccc cacggaccgt
     2761 gacctttgcc cggggaacat ctcaaaatgc cccgccatca ggggcacgga ggagtgtctc
     2821 gcgtcgggtg ggaccacctt cactgtctcc ttccccgtca ggaagagatc ggcctccgtc
     2881 gatgaagcct gatcccagcc tctcttcccg cgacgggcgc actctaagcg tcaccgtcac
     2941 ggatatcgcc atgccgggcg acgcgggggt cgcaaaggcc ggtgatttcg tggtcttcgt
     3001 tcccggcacc attcccggcg ac
//
LOCUS       DAOQTR010000262         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38020_length_3018_cov_3.591968, whole genome shotgun sequence.
ACCESSION   DAOQTR010000262 DAOQTR010000000
VERSION     DAOQTR010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38020_length_3018_cov_3.591968"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKN85_06995"
     CDS             complement(<1..697)
                     /locus_tag="PKN85_06995"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNT44192.1"
                     /translation="MTDHIYWKNGVLYLLDQRELPFRKTYVKCSTAGDVITAINNMTV
                     RGAPLLGIVAAYGMVLGVKEVVAAKRSVRRADIDRIAKKLGATRPTAVNLFWALKRMA
                     EAYEGHAGDRDIAEDMLGEALAIHVEDIENNRMLSLYGAELIEKGDTIMTHCNAGALA
                     TGGYGTALGVMRAAHEAGKKIKVIATETRPYMQGSRLTVWELMQEGIPVELVPENHAG
                     LLCSRGAVNKIITG"
     gene            complement(810..2258)
                     /gene="gatB"
                     /locus_tag="PKN85_07000"
     CDS             complement(810..2258)
                     /gene="gatB"
                     /locus_tag="PKN85_07000"
                     /inference="COORDINATES: protein motif:HMM:NF004015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNT44193.1"
                     /translation="MDYGDYEGVMGLEVHAHLLTESKIFCGCSTKFGAEPNSHTCPTC
                     MGLPGALPVLNRKVVEFAIKLGLALHCSINPRSVFARKNYYYPDLPKGYQISQYEEPI
                     CGNGWLDIVMDGKTKRIRILRIHMEEDAGKLVHERTIDTSSYSMVDYNRSSTPLLEIV
                     SEPDLRTPEEATAYLKTLRDILMYLEICDGNMEEGSFRCDANVSVRKKGDEAFGTRAE
                     LKNLNSFRYIERSLDYEIDRQIALIKKGGSVVQETRLFNAEQGVTYSMRGKEEAHDYR
                     YFPEPDLLPVTVDETWVEEVRKTLPELPMEKMERFMKDYGLPRYDVEILASDRELASY
                     FEAVLKLFPEAKTVSNFIMTELLRELKDGNVSPKDSPVTPARLAELLTLIKDGTISNK
                     IGKEIFPEIYRTGASPRDYVKQKGLLQISDEGAIEGIIDAVIARSPKEVADFKAGKEK
                     LIGFFVGQVMRETKGKANPALLNELLLKKLKA"
     gene            2401..>3018
                     /gene="mtgA"
                     /locus_tag="PKN85_07005"
     CDS             2401..>3018
                     /gene="mtgA"
                     /locus_tag="PKN85_07005"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNT44194.1"
                     /translation="MQELATRKVKKKKPGKLLRFIKVTIVLVVLLVVGFAGFYMVYPD
                     VSKLKKQNPGKTSFMEYREAEYRAKGNKIRIQSKWVPLRSISPYLMKAVLIAEDDKFW
                     SHHGFDTEAIQKALEKNLEAGRFKLGGSTISQQLTKNLYLTPSKNPVRKLKEAIITWR
                     LERALSKRRILELYLNVVEWGEGIFGAEAAARRHFGKSASALTAEE"
BASE COUNT          566 a          895 c          902 g          655 t
ORIGIN      
        1 cgccggtgat gatcttgttc acggcccccc gggagcacag gagcccggcg tggttctcgg
       61 ggacgagttc gacggggatg ccttcctgca tgagctccca caccgtaagg cgcgacccct
      121 gcatgtaagg gcgcgtttcc gtggcgatga cctttatctt cttgcccgcc tcatgggcgg
      181 cgcgcatgac gccgagcgcc gtgccgtagc ctcccgtggc aagcgccccc gcgttgcagt
      241 gcgtcatgat ggtgtcgcct ttttctatga gctccgcgcc gtagagggaa agcatcctgt
      301 tgttctcgat gtcctcgaca tggatggcca gcgcctcgcc gagcatgtct tcggcgatgt
      361 ccctgtctcc ggcatgcccc tcataggctt ccgccattct cttcaaggcc cagaagaggt
      421 tcaccgccgt cgggcgcgtg gcaccgagct ttttggcgat gcggtcgata tcggcccggc
      481 ggacggaccg cttcgcagcc acgacctctt tgacccccag gaccattcca taggcggcga
      541 cgatgccgag cagcggcgca ccccggacgg tcatgttgtt gatggccgtt atgacatccc
      601 cggcggtgct gcatttcaca tatgttttcc tgaagggcag ctctctctga tcgagaaggt
      661 aaagaacgcc gttcttccag taaatgtgat ccgtcatgcg tcacctcttt ctgttgatgg
      721 tcatcccgga cccgatccgg gatccagagg ttcttccgtt gcgcgcttct tcctggattc
      781 cggcctaaag ccggaatgac gaaaaaaatt taggccttca gctttttcag caggagttcg
      841 ttgaggagcg ccgggttggc cttgcctttc gtttccctca tgacctggcc gacgaagaag
      901 ccgatgagct tctccttgcc ggccttgaag tcggccactt ccttcggtga ccgggcgatg
      961 accgcgtcga tgatgccctc gatggccccc tcgtcggaga tctggagcag gcccttctgc
     1021 ttcacgtagt cacgcggcga ggcgccggtc cggtagatct ccgggaatat ctccttgccg
     1081 atcttgttgc tgatggtgcc gtccttgatg agcgtgagaa gttcggcaag ccgtgccggc
     1141 gtaacggggg agtccttcgg ggaaacgttg ccgtccttca gttccctcaa cagctccgtc
     1201 atgatgaagt tgcttaccgt tttcgcctcg ggaaagagct tgagaaccgc ctcgaaatac
     1261 gaagccagct ccctgtcgga ggcgagtatc tccacgtcgt accgggggag gccgtagtct
     1321 ttcatgaagc gttccatctt ctccatgggg agttcgggca gggttttccg cacctcttcg
     1381 acccatgttt cgtccacggt caccggaaga aggtcaggtt cggggaaata gcggtagtcg
     1441 tgcgcctcct ccttgccgcg catggaatag gtgacgccct gctcggcgtt gaagaggcgt
     1501 gtctcctgaa cgacgctccc acccttcttg atgagggcga tctggcggtc gatctcgtag
     1561 tcgagggacc tttcgatgta gcggaaggag ttcaagttct taagctccgc ccgggtgccg
     1621 aaggcctcgt cgcccttctt gcgcaccgag acgttggcgt cgcagcggaa gctgccttcc
     1681 tccatgttgc cgtcgcagat ctcgaggtac atgaggatgt cgcgcagggt cttgaggtac
     1741 gccgtcgcct cctccggtgt cctcaggtcg ggttcgctga cgatctcgag gagcggcgtg
     1801 ctcgagcggt tgtagtccac catgctgtaa ctgctcgtgt cgatggtccg ctcgtggacg
     1861 agcttgcccg cgtcctcctc catgtggatg cgcaggatgc ggatgcgctt cgtcttcccg
     1921 tccatgacga tgtcgagcca tccgttgccg cagatgggct cttcgtattg agagatctgg
     1981 taccccttcg gcaggtcggg atagtagtag ttcttccggg cgaagacgct cctgggattg
     2041 atggagcagt gaagggcgag ccccagcttg atggcgaact cgacgacctt cctgttgagg
     2101 accggcaggg cacccggcag gcccatgcag gtggggcagg tatggctgtt cggctcggcc
     2161 ccgaacttcg tcgaacagcc gcagaatatc ttgctctccg taagaagatg ggcatggacc
     2221 tcgagaccca ttaccccttc gtagtcacca taatccacgg cgcacctctc tgactgcttt
     2281 ctatcgctta caaatgattg aaactataca acaggtaacg gtgtcatttc aaaacaaatt
     2341 gcccccttaa gggagaaagg gaccttccaa tggcgcccgc gatatgataa gaagagatgg
     2401 gtgcaggaat tggcaacaag aaaggtcaaa aagaagaagc cggggaaact cctgcgcttc
     2461 atcaaggtca ccatcgttct cgtcgtcctt ctcgtggtgg ggttcgcggg tttctacatg
     2521 gtctaccccg acgtgtcgaa gctgaaaaag cagaacccgg gtaagacgtc cttcatggaa
     2581 taccgggaag cggagtaccg ggcgaaggga aacaagatac ggatacagtc gaagtgggtc
     2641 cccttgcggt ccatatcgcc ctacctcatg aaggcggtcc tcatagcgga ggacgacaag
     2701 ttctggtccc accacggctt cgacacggag gcgatccaga aggccctcga gaagaacctg
     2761 gaggcgggac ggttcaaact cggcggcagc acgatcagcc agcagctcac gaagaacctc
     2821 tacctcacgc cctccaagaa ccccgtccgc aagctcaagg aagcgatcat cacctggagg
     2881 ctcgagaggg ccctctcaaa acgtaggatc ctcgagcttt acctcaacgt cgtcgaatgg
     2941 ggcgagggta tcttcggcgc cgaggctgcc gcgaggcgcc acttcggcaa gtcagcctcg
     3001 gcgctcaccg cggaagag
//
LOCUS       DAOQTR010000263         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38064_length_3016_cov_2.621074, whole genome shotgun sequence.
ACCESSION   DAOQTR010000263 DAOQTR010000000
VERSION     DAOQTR010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38064_length_3016_cov_2.621074"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKN85_07010"
     CDS             complement(<1..241)
                     /locus_tag="PKN85_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44195.1"
                     /translation="MTHSFTKRTCRALPVMAAAVIILCLTLPATAATAQKTPKPLRIV
                     SLSPAITESLYLLGLGDNIIGVTTYCNKPGQARTKE"
     gene            complement(238..921)
                     /locus_tag="PKN85_07015"
     CDS             complement(238..921)
                     /locus_tag="PKN85_07015"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HNT44196.1"
                     /translation="MNDRKDAFCSWSGGKESALALFRARRSGFTITRLINMVTEDGTH
                     SRTHGLEADLLYLQARAIGIPLTQRRTTWNGYEEEFKKVLSLLNAEGILQGVFGDIDM
                     EEHRAWVERVCSDCDVRPSLPLWLEKREDILSEFIDEGFKAVIVAVDRRYLDDRWVGR
                     EIDRGFVDDIRALGGDVDICGEKGEYHTFVYDGPIFQRPVPFERGGVRSTDDYFFLDI
                     KALPLKDGR"
     gene            complement(921..1832)
                     /locus_tag="PKN85_07020"
     CDS             complement(921..1832)
                     /locus_tag="PKN85_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNT44197.1"
                     /translation="MLRRALQSLFARFAADCVFFSGGLDSSIAAVLSGCKKGILVTLD
                     RDGPDLRYAGEAAGAIGLKLIHKAVSVDEALAAIPEVIATLRSFDPAIPNDLAVWFAL
                     KEAKALGMSSVMTGDGADEIFGGYSYMAEIGDLEEYIRYLSQTMSFNSTILGRHLGVE
                     VVQPFLDKEILDFALQIQGKWKIREEKGVLHGKWVLRRACEGLLPPGILWQGKRPLET
                     GSGMTRLNAIMDTTISDEEFEDKRHSSGMRFYNKAHLYYYEVYRKTVGDIPPPGPHEQ
                     ACEGCGAGVKAGRGHCRVCGWVKEAVQ"
     gene            complement(1867..>3016)
                     /locus_tag="PKN85_07025"
     CDS             complement(1867..>3016)
                     /locus_tag="PKN85_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT44198.1"
                     /translation="KPADTVLLSLGMAYRKRHTSFHYDTTGGPWDSMRRFETTSFTPK
                     VTVSEDIFSLRNTLVAGLDYYYSPSRSNDLGTGTDSDTNINKTEYGFYINDEIYLFKN
                     LQFSAGYRLAKVKYDFDYTDNTGFLAPINDFVKKDKDALRLGLNFIIPERGNIFLNYA
                     QGFRFPATDEYFSPFAVPPINEALEPHTVKEIDLGIRFKFTKNIGGAATVFSGRHKNE
                     IYYNPLTFTNTNYGKTRREGIEASFFWLVVPGLRLDLGYTYMDARFDGDDLDGNRVPM
                     VPKNKLSAAVAWAFKDLTLTFSSLYTGDRYMTSDVRNLYSAMGGFTVYDLTAVYAWKG
                     LKLTGGVRNLFDKQYSEYAVVNNTTGARNYYPSPERNYILGVQYSYSF"
BASE COUNT          598 a          832 c          885 g          701 t
ORIGIN      
        1 tttcctttgt gcgggcctga cctggcttgt tgcaataggt ggtcacgccg atgatgttat
       61 cccccagtcc gagaagatag agggattccg tgatggcggg gcttagggag acgatgcgaa
      121 ggggtttcgg cgtcttttgc gccgtggcgg ccgtggccgg cagggtgagg cacaggatga
      181 tgaccgcggc ggccatgacg ggcaaggccc ggcacgttct tttggtgaat gaatgggtca
      241 ccggccgtcc ttcaagggca atgccttgat atcaaggaag aaatagtcat ctgtcgagcg
      301 gacgccgccc ctttcgaaag ggacgggtct ctggaatatg ggaccgtcgt agacgaaggt
      361 gtggtattcc cccttctcgc cgcatatgtc cacgtcgccg ccaagggccc ggatatcatc
      421 gacgaacccc cggtctatct ccctgccgac ccacctgtcg tcgagataac gtctgtcgac
      481 ggccacgatg accgccttga agccttcgtc tatgaattcc gagaggatat cctccctctt
      541 ttcgagccac agggggaggg aagggcgcac atcacagtcg gagcagaccc tttccaccca
      601 ggccctatgt tcttccatgt cgatgtcccc gaagaccccc tggagtatcc cttcggcgtt
      661 gagcagggac agcactttct tgaattcctc ttcataacca ttccacgtgg tcctgcgctg
      721 cgtgagaggg atgccgatgg cccgcgcctg aaggtacagg aggtccgcct caaggccgtg
      781 cgtcctcgag tgcgtgccgt cctcggtcac catgttgatg agcctggtta tggtgaaccc
      841 cgaccttctg gccctgaaaa gagccagggc actctctttt ccaccgctcc aggagcagaa
      901 cgcatccttg cgatcgttca ttattgaaca gcctccttga cccatccgca taccctgcag
      961 tgaccgcggc ctgcctttac tcccgcgccg catccctcac aggcctgttc atgcggcccg
     1021 ggtggaggga tatcccccac ggtcttcctg tagacctcgt aatagtacag atgggccttg
     1081 ttgtagaagc gcatgcctga ggagtgcctt ttgtcctcaa actcctcgtc ggatatggtg
     1141 gtgtccatta tggcgttgag ccttgtcata cccgaccctg tctcaagcgg tctctttccc
     1201 tgccagagaa taccgggtgg cagaagccct tcacaggcgc gtcgtaagac ccacttgccg
     1261 tgaagtacgc ccttttcttc cctgatcttc cattttccct gtatttgcag ggcgaaatca
     1321 aggatttctt tgtcgagaaa gggctgtacc acctcgacgc ccaggtgtct tccaagaatg
     1381 gtcgagttga aggacatggt ctgtgagagg taacggatgt attcttcaag atccccgatc
     1441 tctgccatgt aagaatagcc gccgaaaatc tcgtcggcgc cgtcgccggt catgacggaa
     1501 ctcattccca gcgcctttgc ctccttaagg gcgaaccaaa cggcgaggtc gttggggatc
     1561 gcggggtcga agctgcgcaa ggtggcgatg acctcgggta tggcggcaag ggcctcatcg
     1621 acacttacgg ccttgtggat gagtttgagg ccgatggcgc ctgccgcctc gccagcgtac
     1681 cggagatcgg ggccgtcccg gtcgagggtt accaggatgc ctttcttgca cccggaaagg
     1741 acggcggcta tggaagagtc caggccgccc gaaaagaata cgcaatcagc ggcaaatcgg
     1801 gcaaagaggc tctggagtgc ccgacgtagc attagtatga cgatgtcctc tgcggaccgc
     1861 gtatcgctag aaactgtagc tatactgcac ccccaggata tagtttcgtt cgggtgaggg
     1921 gtagtagttc cgcgcacccg tagtgttgtt gaccacggcg tactcgctgt attgcttgtc
     1981 gaagaggttc ctgaccccgc ccgtgagctt caggcccttc cacgcataca ccgctgtaag
     2041 atcgtaaacg gtgaaacccc ccatggcgga gtagaggttt ctcacgtcac tcgtcatgta
     2101 gcggtctccg gtgtacaggg acgaaaatgt aagggtcagg tccttgaagg cccaggcgac
     2161 agcggcgctc agtttgttct ttggaaccat gggaacacgg ttcccgtcca ggtcgtcccc
     2221 gtcaaagcgg gcgtccatgt aagtgtagcc aaggtccaac ctcaggccag ggacaacgag
     2281 ccagaagaag gaagcctcga tcccctccct ccgtgtcttt ccgtaattgg tgttcgtgaa
     2341 tgtcaacggg ttatagtata tctcattctt gtgacgtccg gaaaaaacgg ttgccgcccc
     2401 cccgatattc ttcgtgaatt tgaacctgat gccgaggtcg atctctttta cggtgtgggg
     2461 ctcgagggcc tcattgatgg ggggtaccgc gaagggactg aagtattcgt ctgttgccgg
     2521 gaaccggaag ccctgcgcgt aattgaggaa gatgttcccc ctctcgggaa tgatgaagtt
     2581 gagccccaac cgtaaggcat ccttgtcctt ctttacaaaa tcgtttatgg gagcgagaaa
     2641 acccgtgttg tcggtgtagt cgaaatcgta ttttaccttg gccagacggt aaccggcgct
     2701 gaattgcagg ttcttgaaga ggtatatctc gtcgttgatg tagaaaccgt actccgtctt
     2761 gttgatgttc gtgtcggagt cggtgcctgt gccgaggtcg ttgctgcggc tgggggagta
     2821 atagtaatcg agaccagcga cgagagtgtt ccttagggaa aagatatctt cgctgacggt
     2881 gaccttcggg gtgaaagatg ttgtctcgaa ccttctcatc gaatcccagg gcccgcctgt
     2941 cgtgtcgtag tggaacgagg tatgtcgttt ccggtaggcc atgcccaggg aaaggagcac
     3001 tgtgtcggca ggcttc
//
LOCUS       DAOQTR010000264         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38115_length_3013_cov_3.346180, whole genome shotgun sequence.
ACCESSION   DAOQTR010000264 DAOQTR010000000
VERSION     DAOQTR010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38115_length_3013_cov_3.346180"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..572)
                     /locus_tag="PKN85_07030"
     CDS             complement(222..572)
                     /locus_tag="PKN85_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNT44199.1"
                     /translation="MGLLLKWLIMAASIIIAAYFIPGVRVGSFFSALWVALFLGVVNI
                     LVRPVLILITLPINILTLGLFTFVINAVLVLLASSVIKGFEVAGFWWALLYSVVLSLV
                     NYVINLIVQPKEDR"
     gene            complement(591..872)
                     /locus_tag="PKN85_07035"
     CDS             complement(591..872)
                     /locus_tag="PKN85_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44200.1"
                     /translation="MSDIMTVDELLASKNLTREEWELHRDLIEDCRKNETRINECCAE
                     TRDNIEKMAGILDAISVKMVALSVALETIIGEAEDVSLRMLPEHKFFRE"
     gene            1089..1856
                     /locus_tag="PKN85_07040"
     CDS             1089..1856
                     /locus_tag="PKN85_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNT44201.1"
                     /translation="MKDKGKDKAGIGIRFQNETKYTPDSLSGHVFNLGAIPDTFKEYP
                     NPIARVSLPEPKAGGESGLWKLLLKRRSRREYDAGKPLKLNDLSALLWATQGLTAQFG
                     DTFFRTAPSAGGLYPVETYLCVRSVEGLEEGIYHFRPGDYDLELLKKGDHSKALTEAA
                     LQQSLILTAEVTFIWSAVIARGSWKYGQRVYRYIYLDAGHIAQNLYLAGEALGLGVCA
                     VGAFYDDDVNHIVGLDGEEETVIYMAAVGFRSARNPL"
     gene            1880..2935
                     /locus_tag="PKN85_07045"
     CDS             1880..2935
                     /locus_tag="PKN85_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT44202.1"
                     /translation="MYEQGVIRPPSEAQSLLIRVTRNCPWNQCIFCPAYKGVKFSRRT
                     VDEVKKDIDSMEKEYASYKTMIRSAFLQDADSLVLKTSDILEIINYTKEKFPALERIT
                     TYARATTLKRKTVEELRELCKAGLTRIHVGLESGSEKVLKMIRKGITPDDIVEGGRKV
                     VQAGMSLSEYIMPGVGGRTMSSENAIETARLLNQIKPDFIRVRTFAMHPMSPMQKLVE
                     DGTFVVMNDAEIVAEIRLLVENLDEMHTYFSCGDFSLNLLMQVDGYLDEKKAYMLSEL
                     DRFLALTSEQQKAYALIRRSSYMQYPLEAAENEDLLSRIRPEIEKLEKDDPEGFYKYI
                     ETLKSYQLPQPQTEEWK"
BASE COUNT          742 a          878 c          828 g          565 t
ORIGIN      
        1 atcgccggac ggtcatcacg ggttccttca acttcaccta cgcagccgaa gaaaaaaacg
       61 ccgagaacct tctcatcata acctccgggg agctggccgc cctttacagg gacaaccatc
      121 tgaagctcag gaaacactcg aaaagatact aggaactgaa agaccgttag aaccgttgga
      181 atcgttagag accgttgacg gcccttcccc ttccaacggt tctaacggtc ttctttcggt
      241 tgtactatca ggttgatgac gtagttaaca agggacagca cgacgctgta gagcagggcc
      301 caccagaaac ctgccacctc gaagcccttg atgaccgatg acgcgaggag aacgagaacg
      361 gcgttgatga cgaacgtgaa cagtccaagg gtgagaatgt tgatgggcag ggtgataaga
      421 atgaggaccg gccgcacgag gatgttcacg acgcccagaa agagggccac ccagagggcg
      481 gagaagaaac tgccgaccct cacgccggga ataaagtagg ccgcaataat gatggacgcg
      541 gccattatca accatttgag aagaagcccc atgacccggg aagtgtggac ctattcgcgg
      601 aagaacttgt gctccgggag catcctcagg gagacgtcct cggcctcccc tatgatagtc
      661 tcgagagcca cactcaaggc caccatcttt accgagatgg catcgaggat accggccatc
      721 ttttcgatgt tgtccctggt ctcggcgcaa cattcgttga tcctggtctc gttcttcctg
      781 cagtcctcga taaggtccct atgcagttcc cactcttctc gcgtcaggtt cttcgaagcc
      841 agaagctcgt ctacggtcat gatatcgctc atgttaacct ccatccccga aaacaggtgc
      901 cagtgattct gtaactaagt ctatcttttt tggccggaaa aggcaaacgg aaaggcaggg
      961 aaccgtatag gtcctatcca gtcctatggg tcctattgaa gacccccctc gctccccctg
     1021 cccctgcctc tatcattgac ttatcccacc gcatgaagca tattaaaggt gtatttgaaa
     1081 ggtggacagt gaaggataag gggaaggata aggccggcat agggatccgg tttcagaatg
     1141 agaccaagta tacgcccgat tcactttccg gccacgtgtt caacctcggg gcgatccccg
     1201 acacgttcaa ggaatatccg aaccccatcg ccagggtctc gctgccggag cccaaagccg
     1261 gcggggagtc gggcctgtgg aaacttctcc tgaagagaag gtcgcgccgc gaatacgacg
     1321 cgggcaaacc cctcaagctc aacgacctct ccgcgctgct gtgggcaacg cagggtctga
     1381 cggcgcagtt cggcgacacc ttcttccgga cggcgccctc cgcgggcggc ctctaccccg
     1441 tggagaccta cctgtgtgtc cgttccgtcg agggactcga agaggggata taccatttca
     1501 ggcccggcga ctatgacctg gaactcctca agaagggcga tcactcaaag gccctcaccg
     1561 aggcggccct tcagcaatcc ctcatcctca ccgctgaggt caccttcatc tggtcggccg
     1621 tcatagcgcg gggaagctgg aaatacggcc agagggtcta ccggtacatc tatctcgacg
     1681 ccggtcatat cgcgcaaaac ctttacctcg ccggggaggc cctgggactc ggagtctgcg
     1741 ccgttggcgc attctacgat gacgacgtga accacatcgt cggcctcgat ggagaagagg
     1801 aaaccgtcat atacatggcc gcggtcggat tccgctccgc gcggaatccg ctgtgacaga
     1861 actaaggagc ccaaggaaca tgtacgaaca aggagtcata agaccaccca gcgaagccca
     1921 gagcctcctg atacgggtta cgaggaactg cccgtggaac cagtgcatct tctgccccgc
     1981 ctataagggg gtgaagttct cccgccggac agtcgacgag gtcaagaaag acatcgacag
     2041 catggagaag gaatacgcct cctacaagac catgatcaga tcggcgttcc tgcaggacgc
     2101 cgacagcctc gtcctgaaga cctcggacat cctcgagatc atcaactata cgaaggagaa
     2161 gtttcccgca cttgaacgca tcacgaccta tgcccgggca acgacattga aacgcaagac
     2221 tgtggaagag ttgcgcgaac tctgcaaggc gggcctcacg aggattcacg tcggactgga
     2281 gagcggctcg gaaaaggtgc tcaagatgat acggaagggg atcacgcccg atgacatcgt
     2341 cgaaggcggc aggaaggtcg ttcaagcggg gatgtcgctg tcggaataca taatgccagg
     2401 cgtgggcggc aggacgatga gctccgagaa cgccatcgag acggcgcgtc ttctcaacca
     2461 gatcaaaccc gacttcatca gggttcggac cttcgcgatg caccccatgt cgccgatgca
     2521 gaagctcgtt gaggacggga ccttcgtcgt tatgaacgac gcggagatag tggcggagat
     2581 acgcctcctc gtggaaaacc tcgacgagat gcacacctac ttctcctgcg gtgatttcag
     2641 cctcaacctg ctcatgcagg tggacggcta tcttgacgaa aagaaagcct acatgctcag
     2701 cgagctcgac aggttcctcg ccctgacatc ggaacagcag aaggcctatg cccttatcag
     2761 gcgctcctcc tacatgcaat accccctcga ggccgcagag aacgaggacc ttctgagcag
     2821 gatacgcccc gagatcgaaa agctcgagaa ggacgacccc gagggcttct acaagtacat
     2881 agaaacgctg aaatcctacc agctccccca accgcagacg gaggagtgga agtaaaggcg
     2941 gcttgaattg cttgagtagc ttgaatcgct tgacagcctg ttgataaacc ccaccagccg
     3001 gccctctttt ccc
//
LOCUS       DAOQTR010000265         3006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38263_length_3006_cov_2.933921, whole genome shotgun sequence.
ACCESSION   DAOQTR010000265 DAOQTR010000000
VERSION     DAOQTR010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38263_length_3006_cov_2.933921"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1790)
                     /locus_tag="PKN85_07050"
     CDS             complement(132..1790)
                     /locus_tag="PKN85_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44203.1"
                     /translation="MRGYEELEGELRDTRARLDEAEETLRAIRNNEVDALVVEGDRGV
                     QVFTLQGAEQPYRTLMETMAEGALTMATDGTILYCNRQLATMLGAPLNRIIGRSLREI
                     VTSKDWQALQGVLDACGASGCRGEYRLITSGGREITTSVSACPIQLQGVECLCLVISD
                     LTEQRSFEQQLRRAQKLEALGTLAGGIAHDFNNVLAGIIGFAEMVLEDTSPDRPEHRR
                     LTMVLKAAHRGRDLVKQILLFSRRGEQDRKPLALVEVVEDTLKLLGPAFPSTIEILWK
                     GTADDCRILADAAQMHQVVANLCTNAVHAMRETGGILQIVASRAIVAEGTPPPLPEMA
                     PGEYVVLEVSDSGCGMDPETMEQMFDPFFTTKGEGEGTGLGLSVSHGIVKGHGGYIAV
                     ESEPGIGSRFLVYLPGFAEGADIQVESASSSPLGGRERILIVDDEDILVELNRQRLSG
                     LGYDVTATTSSLDALQMFGRAQDSFDLVITDETMPHMTGMDLAAELLKAKPGIPIILC
                     TGHSDKVSPERAWEAGIQKLLMKPFDKRELAEAVRQLLDKDKKS"
     gene            complement(1802..2167)
                     /locus_tag="PKN85_07055"
     CDS             complement(1802..2167)
                     /locus_tag="PKN85_07055"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNT44204.1"
                     /translation="MTKKTRDQVEVFEEALKGAQDKKYVLRLYVAGSTPRSTDAILGI
                     KKICEEYLKGRYELEVIDIYQQPTLARGEQIIAAPTLVKKLPPPLRKFIGNMGDVDRI
                     LVGLDLKSRDGVEPKDEKE"
     gene            complement(2185..2499)
                     /locus_tag="PKN85_07060"
     CDS             complement(2185..2499)
                     /locus_tag="PKN85_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNT44205.1"
                     /translation="MSNEELQGPEDGMDFWQLRLYVAGQTPRSLTAFANLKRICEEHL
                     AGRYRIEIIDLLENPTLAKGDQILAIPTLVRKLPEPVRKIIGDLSNTERVLVGLDLRR
                     LS"
     gene            complement(2496..>3006)
                     /locus_tag="PKN85_07065"
     CDS             complement(2496..>3006)
                     /locus_tag="PKN85_07065"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNT44206.1"
                     /translation="TFCGLEMHLLGMTKLVNEFKPHVVVVDPITNLISVGLPADVRSM
                     LTRLTDLLRTNQATSVFTSLTAIGPGLDQSELGISSIMDTWITVKNIEVQGKRKRALT
                     IVKSRGMNHSNESREFWLTERGLSLEEDPPRAPGTSNARERTPPRKGDRLRASRAPGL
                     RGTGKEKGR"
BASE COUNT          558 a          936 c          812 g          700 t
ORIGIN      
        1 gtgaattcga gcgtggtggg tatgggtttt gccttctgat gggccggata gatgacaagg
       61 atctcaagga actggtacct ttctcctggc ggaggcccaa aggggagatg gaggaaacga
      121 cggcttgcct gtcagctttt cttatccttg tcgagcaact ggcggaccgc ctctgccagt
      181 tcgcgcttgt cgaagggttt catgaggagc ttttggattc ctgcctccca tgcacgttcc
      241 ggtgagacct tgtcgctgtg acccgtgcaa agaatgatcg gaatgcccgg tttagctttc
      301 agcagttccg ccgcaaggtc catccccgtc atatgcggca tggtctcgtc cgttatgacg
      361 aggtcgaagc tgtcctgtgc ccgcccgaac atctgcaggg cgtccaggct gcttgtcgtc
      421 gcggtcacat catagcctaa accgctcaat ctttgtcggt tcaactcaac caggatgtcc
      481 tcgtcgtcga cgatgaggat gcgctccctc ccgcccaatg gggaagaaga cgcgctctca
      541 acctggatgt ccgcaccctc cgcgaacccg ggcaaataca ccaggaacct cgacccgatt
      601 cccggttcgc tctcaaccgc tatatatccg ccatgaccct tcacgatgcc gtgggagacc
      661 gacagtccca gacccgtccc ctccccttct cccttcgtag tgaagaaggg atcgaacatc
      721 tgctccatgg tttcggggtc cattccgcaa ccgctgtcgc tcacttccag caccacgtat
      781 tcacccggcg ccatttcagg gagagggggc ggcgttccct ccgcaacgat agcccgtgaa
      841 gcgactatct gtaatatgcc ccccgtctca cgcatggcat gaaccgcatt cgtgcacaaa
      901 tttgcaacca cctgatgcat ttgcgcagca tcggcgagta ttcggcaatc atccgctgtt
      961 cccttccaga gaatctctat ggtggacggg aatgccggtc cgagcagttt gagagtatct
     1021 tcaacgacct ctaccaatgc cagcggtttc ctgtcctgtt ctccgcgacg actgaaaagg
     1081 agtatctgtt tcaccaggtc acgccccctg tgggccgctt tcaataccat tgtgagcctt
     1141 ctgtgttccg gcctgtcagg actggtatct tcaaggacca tttcggcgaa accgataatg
     1201 cctgcgagga cattgttgaa atcatgggcg atgccgccgg caagggtgcc gagggcctcc
     1261 agcttctggg cccggcggag ctgctgctcg aagctgcgct gttccgtaag atcgctgata
     1321 acgagacaga gacactccac gccctgaagc tggatggggc acgccgatac ggaagtcgtt
     1381 atctcgcgtc ccccactggt gatgaggcga tactctcccc ggcagcctga cgccccgcac
     1441 gcatcaagga caccttgaag cgcctgccag tctttagaag tcactatctc ccgaagggac
     1501 cgcccgatga tcctgttgag cggggcccct agcatcgtgg ccaactgccg gttgcagtaa
     1561 aggatcgtgc cgtccgttgc catggtgagg gcaccctcgg ccatggtctc catcaatgtc
     1621 ctgtagggct gctctgcgcc ctgaagcgtg aatacctgaa cgcccctgtc cccctccacg
     1681 acaagggcat cgacctcgtt gttcctgatc gcccggagcg tctcctccgc ctcgtcgagg
     1741 cgtgcccggg tatccctcag ttcgccctcg agttcctcgt agcctctcat cttatccacg
     1801 gtcactcttt ctcgtctttc ggttcgaccc catccctgga cttgagatcg aggccaacca
     1861 ggatccggtc cacgtcaccc atattgccga tgaactttcg cagaggggga ggaagcttct
     1921 tgaccagcgt gggggccgca atgatctgtt cgcccctggc gagtgtcggc tgctggtaaa
     1981 tatcgatgac ctcaagctcg taccggccct tcaggtactc ttcacagatc ttcttgatac
     2041 ccaggatggc atccgtcgac ctcggtgtac tgcctgcgac gtacagacgc aaaacatact
     2101 tcttatcctg tgcaccctta agagcttcct caaagacttc cacctggtcc cgggtctttt
     2161 ttgtcacaat tgtttccttc ctttctagga aaggcggcga aggtcgagac caacgagcac
     2221 cctctccgtg ttggacaggt ccccgatgat cttccttacc ggctcaggca gttttctcac
     2281 gagtgtgggg atggcgagaa tctgatcacc cttcgcaagg gtggggttct ccagaaggtc
     2341 gatgatctcg atccgatacc tgcctgcgag gtgctcttcg cagatccttt ttaggttcgc
     2401 gaaggcggta agcgatctcg gggtctgccc cgcgacatac agtctgagct gccagaaatc
     2461 cataccatcc tcaggtccct gcagttcttc attgctcatc ggcctttctc cttgcctgtc
     2521 ccgcgcaggc ccggcgcgcg gctggcccgg aggcgatctc ccttccgcgg cggcgtcctc
     2581 tccctcgcgt tcgaagttcc tggcgcgcga ggcggatcct cttccagcga gagtcccctt
     2641 tccgtcagcc agaactccct ggattcattg gagtgattca tgcctctcga cttgacgatg
     2701 gtgagcgcgc gcttgcgttt gccctgcacc tcaatgttct tgacagtaat ccatgtgtcc
     2761 atgatggagg agataccgag ttcactctga tcgagaccgg gacctatcgc ggtcaggctc
     2821 gtgaagacgg acgtggcctg attcgttcta agcaggtcgg tcaggcgcgt gagcatcgac
     2881 ctcacgtctg ccggcaggcc gaccgaaatg aggttcgtta tggggtcaac gacaaccaca
     2941 tggggcttga actcgttcac cagttttgtc atcccgagga ggtgcatctc gagtccgcag
     3001 aacgtc
//
LOCUS       DAOQTR010000266         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38277_length_3005_cov_3.483729, whole genome shotgun sequence.
ACCESSION   DAOQTR010000266 DAOQTR010000000
VERSION     DAOQTR010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38277_length_3005_cov_3.483729"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PKN85_07070"
     CDS             <1..848
                     /locus_tag="PKN85_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNT44207.1"
                     /translation="GDMPVALQKALENTCVQKNPWGRSRNKRADWAQKLDFEVPHVSE
                     TESKRLLFVCCIQAYDPRCMVIPVNVARLFNAGGLEFGILGEEEACCGNEIRRIGEAG
                     LFEELEEENKAAFEEYGVKEIVTLSPHCMNVLKKEYGDMGIKVVHYTELLAPLVEEGA
                     IKLPGSFGKKVIYHDPCFLGKQNGVFDEPRAILGAVPDLELLEFSRSREMSLCCEGGG
                     GRMFFEVEATYQRNADVRVLDAVSRGAEIIATACPFCVMTLEDPATEKGLAVKELSEI
                     LTEVL"
     gene            850..1626
                     /locus_tag="PKN85_07075"
     CDS             850..1626
                     /locus_tag="PKN85_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNT44208.1"
                     /translation="MNILVFTKRVAATQEEELRITGEGQAVDLSKVPFKVNDWDNYSV
                     EEAVRIVDKAGGSVTAISIGDAESDEVLRRAIAMGAKDGFLVETGDVLNDPFARGELI
                     RSFIDKEKIPFDIILTGVQSEDDQFGAVGGIAAALMGLPYASMVIGIDAVEADHVVIR
                     RELEGGLQEKVKVTVPCVLSIQSGINEPRYVSIMGIRKASKVERKVFKADGYEKGSPK
                     IELTKWVYPEKKGGATMLDGELADVCGQLLGILKEKGVCQ"
     gene            1623..2555
                     /locus_tag="PKN85_07080"
     CDS             1623..2555
                     /locus_tag="PKN85_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNT44209.1"
                     /translation="MSYTLIVAEVRRGVFEERNLDSLGLGALLGAQSVLLVPDGAYQV
                     PDKFVDAIVKVKAAEEVYLNPMNMGRMLEAAFAAKGKPDNILFTQSSSGMEAAAYAAG
                     LFGMPVITDVSGYDRDAGIFLKSYYSDKVFGQFKTAGVPAILTVRSGSFREFAAEKTA
                     AAAAEAIDAAAVETRTFVEYVEEEKTDIDITKAEFLLSVGRGVGNQGEVSGYEELAGI
                     LKAVLSGSRPVIDKMWLPKARQVGTSGKTVKPKVYLAMGISGAFQHIAGMKDSDCIIA
                     VNKDPEAPIFQYAHYGIVGDIHKVRDKLKELAKG"
     gene            2555..>3005
                     /locus_tag="PKN85_07085"
     CDS             2555..>3005
                     /locus_tag="PKN85_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT44210.1"
                     /translation="MDEKETKVLVVGGGIGGITAALEIASCGINVTMVEESPSIGGRM
                     IQLDKTFPTLDCSTCVLSPKMVEVALNQKIDLLSWAKVMAVKKEGRGFNATILKRSRY
                     VDLKKCTACGVCSGGCPVVMKNEFNVGTGPRKAVYIPFPQAIPNKALI"
BASE COUNT          654 a          845 c          971 g          535 t
ORIGIN      
        1 acggtgacat gcccgtcgcg ctccagaagg ccctggagaa tacctgcgtg cagaaaaacc
       61 cctgggggcg ctcccgcaac aagcgtgccg actgggcgca gaaactcgat ttcgaggttc
      121 cccacgtatc ggaaacggaa agcaaaaggc tcctcttcgt gtgctgtatt caggcatacg
      181 atccgaggtg catggtcatt cccgtgaatg tcgcgcggct ctttaacgcc ggcggcctcg
      241 agttcggcat ccttggtgaa gaggaggcgt gctgcggcaa cgagatccgg cgtatcggcg
      301 aggcgggcct cttcgaagag cttgaggagg agaacaaggc cgccttcgag gagtacggtg
      361 tcaaggagat cgtcaccctc tcgccgcact gcatgaacgt cctgaagaaa gagtacggcg
      421 acatgggcat caaggttgtc cattacacgg agcttctcgc gcccctcgtc gaagaggggg
      481 cgatcaagct gccggggtct ttcgggaaga aggtcatcta ccacgacccc tgttttctcg
      541 ggaaacagaa cggtgtcttc gatgagccgc gcgcgatcct cggcgccgtc cccgacctcg
      601 agcttctcga gttctcccgc tcgcgggaga tgtcactgtg ttgcgaaggc gggggaggaa
      661 ggatgttctt tgaggtcgag gcgacgtatc agcgcaacgc cgacgtccgc gtcctcgatg
      721 ccgtcagcag gggggccgag atcatcgcaa cggcatgccc cttctgcgtc atgaccctgg
      781 aagacccggc cacagaaaaa ggtctcgccg tgaaggagct ttcagaaata cttacggagg
      841 tcttataaga tgaatatcct tgtatttacg aagagggttg ccgcaaccca ggaagaagag
      901 ctgcgcatca ccggggaagg gcaggccgtc gatctctcga aggtaccctt caaggtcaat
      961 gactgggaca actacagcgt ggaagaggcg gtgcgcatcg tcgataaagc gggcggttcg
     1021 gtgacggcca tttccatcgg tgacgccgaa tccgacgagg tcctgcggcg cgccatcgcc
     1081 atgggggcga aggacggctt tctcgtcgaa acgggagatg ttctcaacga ccccttcgcc
     1141 aggggcgagc tcatccgcag cttcatcgac aaggagaaga tccccttcga tatcatcctg
     1201 acaggtgtgc agtcggaaga cgaccagttc ggagccgtcg gaggcattgc cgccgccctg
     1261 atgggtcttc cctatgcgtc gatggtgatc ggcatcgatg ccgtcgaagc ggaccacgtc
     1321 gtgatccgca gggagctcga aggcggcctc caggagaagg tgaaggtgac cgtcccttgc
     1381 gtgctttcga tacagagcgg catcaacgag ccccggtacg tctccatcat gggcatccgc
     1441 aaggcctcga aggtggagcg caaggttttc aaggccgatg gatacgagaa gggctctccg
     1501 aagatcgaac tgacgaaatg ggtctatcct gagaagaagg gtggggcgac aatgctcgac
     1561 ggagagctcg ccgacgtgtg cgggcagctc ctcgggatcc tcaaggagaa gggggtgtgc
     1621 caatgagtta cacattgatc gtggcagagg tgaggcgcgg cgtattcgag gagaggaacc
     1681 tcgactcgct gggccttggt gcccttctcg gagcgcagtc ggtccttctc gtccccgacg
     1741 gggcctatca ggtgccggac aagttcgtcg acgccatcgt gaaggtaaag gcagccgaag
     1801 aggtgtacct caatcccatg aacatgggca ggatgctgga agccgccttt gccgcgaaag
     1861 gcaagccgga caacatcctc ttcacccagt cctcatcggg gatggaagct gcggcatacg
     1921 ccgcgggcct tttcggaatg cccgtgataa cagacgtgag cggctatgac agggatgccg
     1981 gcatcttcct gaaaagctac tactcggaca aggtgttcgg gcagttcaag acggcgggag
     2041 tccccgccat cctcaccgtg aggagcggga gcttccggga gttcgccgcg gaaaagaccg
     2101 ccgccgctgc agctgaggcc atcgacgccg ccgccgtcga gacccgcacg ttcgttgaat
     2161 acgtcgagga agagaagacg gacatcgaca tcacgaaggc ggagttcctc ctttccgtgg
     2221 gaaggggcgt cggcaaccag ggcgaggtct ccggctacga ggagctcgcg ggcatcctga
     2281 aggcggtcct ttccggatcg cgtcccgtca tcgacaagat gtggctgccc aaggcgcggc
     2341 aggtggggac gtcgggcaag acggtgaagc cgaaggtata ccttgccatg ggcatatcgg
     2401 gcgccttcca gcacatcgcg ggcatgaagg actcggactg catcatcgca gtcaacaagg
     2461 acccggaggc ccccatcttc cagtatgccc attacggcat cgtcggggac atccacaagg
     2521 tgcgtgacaa actaaaagag ctggcaaagg gctgatggac gaaaaagaaa caaaagttct
     2581 cgtggtgggt ggagggatag gcggcatcac cgccgccctg gagatcgcct cctgcggcat
     2641 caatgtgacc atggtggaag agagtccctc catcgggggc aggatgatac agctcgacaa
     2701 gaccttcccc accctcgact gttccacctg cgtcctttcg ccgaagatgg tggaggtcgc
     2761 gctgaaccag aagatagacc tcctgtcatg ggcgaaggtc atggcggtga agaaggaggg
     2821 cagggggttc aacgccacca tcctgaaaag gtcccggtac gtggacctga agaaatgcac
     2881 cgcctgcggc gtctgttccg gaggctgtcc ggtggtgatg aagaacgagt tcaacgtggg
     2941 aacgggaccg cggaaggccg tctacatacc ctttccccag gccattccca acaaggccct
     3001 catag
//
LOCUS       DAOQTR010000267         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38412_length_2998_cov_4.219844, whole genome shotgun sequence.
ACCESSION   DAOQTR010000267 DAOQTR010000000
VERSION     DAOQTR010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38412_length_2998_cov_4.219844"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PKN85_07090"
     CDS             complement(<1..1442)
                     /locus_tag="PKN85_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44211.1"
                     /translation="MKAKLEEVLKEKERELKLYRRFLDLRSYCEKKVFPRIDAPIAEH
                     VETLKGIIEKLIPDPKDRTEEMFSGEIFALLGTIYLHDVSLMKEFDWPLNGNILNARD
                     MDVKGVFMNYEIARRLDIPEGAVEAVNALSYAHLVKKVPLETEITDNTSKAIIRNMKV
                     FEYLFNFAHLLVDVFFTDLAHNHLKRYNDHEIALRPGEAVLDVDSREGIISIQFNSRL
                     PYEAHMLESVKRYVESMFIRFKNHVNGKLGFQYREIVWVITSNFYYERDVFRIPRVSP
                     FLDFEGVPVQRWDDSSVVLDRLFNEGSVVVAGEVSAGKSTLLTYFVLPQLTASHENVY
                     YCELWSQPTKELRDVIGKRHKIPRVDDLDIISLCKTLLKGGPCFFLIDGLEKIVLMEQ
                     GEREKLVRFVEFMLAEPGAYFILCGEKETFFDWYRVFQGMQISSLYELKALERTPYGR
                     SLSAPRRVEGDADVVLPHDRPDVGSGEQTSG"
     gene            complement(1524..2426)
                     /gene="secF"
                     /locus_tag="PKN85_07095"
     CDS             complement(1524..2426)
                     /gene="secF"
                     /locus_tag="PKN85_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNT44212.1"
                     /translation="MMELIKDTKIDFISFRKKAFVISAILVAVGVFSFIMISLGKANL
                     SIDFTGGTNVQVRFAERVNIGDLRSALEKGGIGDVQIQEVSGTRDFFIKTKLSDTEKE
                     SLTAKITSILSTQLKGAKFEMLGSNMVGPGVGQSLKKYAIIAIVLALLGIIIYIAWRF
                     TFLSGIAATIATFHDVLAVAGVFYLLNKEFNLLIITALLTIAGYSLQDTVVVFDRIRE
                     NSGKMKSRDDFGNIINASINEVLSRTIVTGISTLISLAAIMIFGGEVLFDFAFALFLG
                     LIVGTYSSIFVASPILYAWRQKIR"
     gene            complement(2437..>2998)
                     /locus_tag="PKN85_07100"
     CDS             complement(2437..>2998)
                     /locus_tag="PKN85_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HNT44213.1"
                     /translation="VITGLVVTLAPTIGPTLGQDSINKGMLATLLATLFVVIFMIVYY
                     RYSGIIANIALGLNLIYLLGVFSAFKATMTLPGIAGIALTIGMGVDSNVLIFERIREE
                     LRLGKTVRAAVDGGYAKAWVAIFDSHITTLITAAVLFIFGTGPIKGFAITLIFGMIIN
                     LFTAVFGSKTIFDWILVKFKPRTLSI"
BASE COUNT          659 a          835 c          829 g          675 t
ORIGIN      
        1 ccgctggtct gctcgccgct gccgacgtcc ggccggtcat gcggcagcac gacgtccgcg
       61 tctccttcga cccttctcgg agccgacagg gatcgcccgt aaggcgtcct ctccagggcc
      121 ttcaactcgt agagcgatga tatctgcata ccctggaaga ccctgtacca gtcgaagaaa
      181 gtctccttct cgccgcaaag tatgaaatag gcgcccggct cggcaagcat gaattcgacg
      241 aaacgcacca gtttctcccg ttccccttgc tccatcaaga cgatcttctc gagaccgtct
      301 atgaggaaga agcatggtcc tcccttgagg agcgtcttgc acagggagat gatgtcgaga
      361 tcgtctaccc gggggatctt gtggcgtttc cctatcacgt cgcgcagctc ttttgtcggc
      421 tgcgaccaga gctcgcaata atagacattc tcgtgagagg ccgtgagctg gggcaggaca
      481 aagtatgtga gcagggtgct cttccctgcc gatacctctc ccgccaccac aacggagcct
      541 tcattgaaga gcctgtcgag gaccacggag gagtcgtccc atcgctgcac cgggacacct
      601 tcaaaatcga ggaagggaga gactctgggt attctgaaga cgtctcgctc atagtagaaa
      661 ttgctcgtga tgacccacac tatttccctg tactggaaac cgagcttccc gttgacgtgg
      721 ttcttgaacc ttatgaacat gctctccacg taccgtttga cactctcgag catgtgcgct
      781 tcgtaaggga gcctgctgtt gaactgtatg gatatgatcc cttcccggct gtcaacatcg
      841 agcaccgcct cacccggcct cagggcgatc tcgtggtcgt tatacctctt gaggtggttg
      901 tgggcaaggt cggtgaagaa gacgtccacg agaagatggg caaagttgaa gaggtactcg
      961 aagaccttca tgttccgtat gatggccttg gaggtgttat ccgttatctc cgtctcgaga
     1021 gggaccttct tgacgagatg ggcgtagctc agggcattga cggcctccac cgcaccttcg
     1081 gggatgtcga ggcgtcgcgc tatctcgtag ttcatgaaga cgcccttcac atccatatcc
     1141 cgggcgttga gtatgttccc gttgagcggc cagtcgaatt ccttcatcag ggaaacatcg
     1201 tggagataaa tggtcccgag cagggcaaag atctccccgg agaacatctc ttcggtgcgg
     1261 tccttcgggt cggggatgag tttctctatg atgcctttca gggtctccac gtgctccgcg
     1321 atgggggcgt cgatccgtgg gaagaccttt ttctcgcaat agctcctgag gtcgaggaac
     1381 ctgcggtaga gcttgagttc cctctccttc tccttcagga cctcttccag cttggccttc
     1441 acgcaacctc tcccgcctgc atgcacctgc agcagatcgt ccgcattata tctttcccgg
     1501 gtgtcctaac ggcaaggtta ccgttaacgg atcttttggc gccatgcata gaggatcggg
     1561 ctcgccacga agatcgacga gtaagtgccc acgatgagcc caagaaaaag cgcaaaggcg
     1621 aaatcgaaga gcacttcccc gccaaagatc ataatggcgg ctagactgat aagggtactg
     1681 ataccggtga cgatcgtcct ggaaaggacc tcgttgatgc tagcgttgat gatattgcca
     1741 aaatcgtcgc gggatttcat cttgcccgag ttctccctta tgcggtcgaa gacgacgacc
     1801 gtgtcctgga gggaatatcc cgctatggtc aaaagagctg tgatgatgag aagattgaac
     1861 tccttgttga gaagatagaa gacacccgct acggccagaa catcgtggaa ggtggcaatg
     1921 gtcgccgcga tgcccgacag gaaggtaaac ctccaggcaa tgtagatgat gatcccgagc
     1981 agggccagaa cgatggcgat gatggcgtat ttcttcagcg actggcctac gccgggaccg
     2041 accatgttgc tccccagcat ttcgaacttg gcgcccttca gctgcgtcga caggatggag
     2101 gttatcttcg cggtgaggga ctccttttcc gtgtccgaaa gcttcgtctt gataaagaaa
     2161 tcccgcgttc cgctgacctc ctggatctgc acatcgccaa tacccccctt ctccagggcg
     2221 gacctgaggt caccgatatt gaccctttcg gcaaacctca cctgcacgtt cgtgcccccc
     2281 gtaaagtcga tgctgaggtt ggccttaccg agagagatca taatgaaaga aaacacccct
     2341 acagccacga gtatcgccga tatgacaaat gccttcttcc taaaggagat gaaatctatt
     2401 ttcgtatctt taatgagctc catcatgggc gccgcctcat atactcagtg ttctgggttt
     2461 gaatttcacg agtatccagt cgaagatggt cttcgatccg aacaccgcgg taaagagatt
     2521 tatgatcatg ccgaagatca gggttatcgc gaaacccttt atcggccctg ttccgaatat
     2581 gaagaggacg gccgccgtga tgagcgtggt gatgtgggag tcgaatatcg ccacccacgc
     2641 cttcgcgtag ccaccgtcca ccgccgcccg gaccgttttc cccagccgca gctcttcgcg
     2701 tatcctctcg aagatgagga cgttggaatc aacgcccatg ccgatggtga gcgcgatacc
     2761 cgctatgccg gggagggtca tcgtcgcctt gaaggcgctg aagacaccga gcagatatat
     2821 gaggttgagt ccgagggcga tattggcgat gatgcccgaa taccggtagt aaacgatcat
     2881 gaatatgaca acgaagaggg tcgcgaggag agtcgccagc atgcccttat tgatcgagtc
     2941 ctgaccgagc gtcggaccga tggtgggcgc cagggtgacc accaggccgg tgatgacg
//
LOCUS       DAOQTR010000268         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38522_length_2992_cov_4.088866, whole genome shotgun sequence.
ACCESSION   DAOQTR010000268 DAOQTR010000000
VERSION     DAOQTR010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38522_length_2992_cov_4.088866"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..835)
                     /locus_tag="PKN85_07105"
     CDS             complement(56..835)
                     /locus_tag="PKN85_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNT44214.1"
                     /translation="MDQGEVIVRKDGRVLYLTIKREKALNALNRNILEGLAVVFEGLE
                     KKRDASVVVMTGYGPKAFVAGADVREIKDAGAGRTEFIRMGQRVFHRIMTSPSVVIAA
                     VNGFALGGGCELALACDIRIASENASIGMPETGIGVMPGYGGTQLLSRLLHPGMAKYM
                     IFSGTPVNASEALQLGLVEKVYPEGVFMEEVEKLARKIASRGPIALECCKRAIDNGVG
                     LPLREALEVEIVEYDKVARSRDAEEGLSAFLEKREPVFTGT"
     gene            complement(907..1308)
                     /locus_tag="PKN85_07110"
     CDS             complement(907..1308)
                     /locus_tag="PKN85_07110"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT44215.1"
                     /translation="MSKECFNVFDEMYKRKKFTWSDAVKKGKVIAISGTVATDENHRT
                     LYAGDLNAQMRYIFESVKNLLEKMGASMDDVIKTTDYITPQAVPDYAKTAGIRREYFK
                     EDSYPAATGVVVHSLLRPDWLIEIDFLAVVD"
     gene            complement(1361..>2992)
                     /locus_tag="PKN85_07115"
     CDS             complement(1361..>2992)
                     /locus_tag="PKN85_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT44216.1"
                     /translation="GRSDVIGMARTLFADPDWPVKAREGRADEIVKCAACGYCSEADE
                     RYETVVCIEWPKGALSAPSPWLLAPPCKAACPAGLDIRSYIDLAAQGEYEKALAVIEE
                     KIPFPASVSRVCPHPCERVCNRGTFDRPIAINGLKRFVVDAVAKAGKREKVEPFPRTR
                     DERVAVIGSGPAGMTAAFELAKLGYPVTVFEAAPVPGGMLALGIPEYRLPKKMVREEI
                     DAIEKRGVEIRLNTPVGEGGMPFGSLREEGFKAIVVAIGAHRSVSPGIEGEGLEGVHH
                     GVGFLKAVNLGVSVTIGEKVAVVGGGNVAVDAARTVHRMGAKEVTIFYRRSKDEMPAY
                     PEEIEAAETEGVKIVFLSAPVEVTGKDGKATGLRCVRTELGAPDESGRRTAHPVKDSE
                     FEIAADCVILAVGETPDAGFFGSAGLPMKVQGDSQWTGQEGIFACGDAVTGPATVIEA
                     IASGRRTATGVHRYMCGESLDYTIAEPAVIPVEDVELERFKKREREKMPALNVPDRVG
                     NFREVELGFTELMASSEAERCFQCGMFPRKDKNIG"
BASE COUNT          524 a         1036 c          758 g          674 t
ORIGIN      
        1 atctgcccac atcccaatac gcccagtttc tttatttcca ttgtgatctc cttatctacg
       61 ttccggtgaa gactggttcc ctcttctcga gaaaggcgga gagaccctct tcggcatccc
      121 gggaacgggc caccttgtcg tactcgacga tctccacctc cagggcttcc cgcaagggca
      181 gccccactcc gttgtcgatg gccctcttgc agcactcgag ggcgatcgga cctctcgaag
      241 cgatcttccg cgcgagcttt tccacttctt ccatgaacac gccctcaggg tagacctttt
      301 cgacgaggcc cagttgcagg gcctcagagg cattcacggg ggttcccgaa aagatcatgt
      361 atttcgccat tccagggtgc agcaagcggg agagcaactg cgttccccca tagccgggca
      421 tcactccaat gcctgtctcg ggcatgccga tggaggcatt ctctgacgcg atgcgtatgt
      481 cgcaggccag cgccagctcg caacctcctc ccagtgcgaa cccgttcacg gcggcaatga
      541 ccacgctggg ggacgtcatt atccggtgga aaaccctctg tcccatcctg atgaactccg
      601 ttctgcccgc gcctgcatcc ttgatctccc gcacgtccgc gccggccacg aaggccttcg
      661 gaccgtatcc ggtcatgacc accacgcttg catcacgttt tttttcgaga ccttcgaaga
      721 cgaccgcaag tccctccagt atgttcctgt tgagagcgtt gagcgccttc tcacgcttta
      781 tcgtgagata gagcactctc ccatcctttc tgacgatcac ctcaccctgg tccatattct
      841 cctctcgtgg gcaggccccg ctgagattcc gcagggcctg ccctgctcac gtcgctcttg
      901 tcgctatcag tccacgactg caagaaaatc gatctcaata agccagtcag gcctgaggag
      961 gctgtgcacc acaactcccg tggcggccgg atagctgtcc tctttgaaat actcccttcg
     1021 tatgccggcc gttttcgcgt agtcggggac ggcctggggc gtgatgtaat ccgtcgtttt
     1081 gatgacgtcg tccatgctcg cgcccatctt ttcgagcagg ttcttgacgc tttcgaatat
     1141 atagcgcatc tgcgcgttaa gatcgcccgc gtagagcgtc cggtggttct cgtcggtggc
     1201 caccgtaccc gagatggcta tgaccttgcc tttctttacc gcatcactcc aggtgaattt
     1261 cttccgcttg tacatttcat cgaacacgtt aaaacattct tttgacattg tggtacctcc
     1321 atctatgttg ttgctggtgc tcgtgttgcc ccggtgcgga ctacccgatg ttcttgtcct
     1381 tcctcgggaa cataccgcac tggaaacacc gctcagcctc gcttgacgcc atcagctccg
     1441 tgaagccaag ctccacttcc ctgaaattgc caaccctgtc gggcacgttg agggcgggca
     1501 tcttctcccg ttccctcttc ttgaaacgct ccagttcgac gtcctccacc gggatcaccg
     1561 cgggctccgc tatcgtatag tcgagggatt caccgcacat gtacctgtga acacccgtcg
     1621 ccgtccggcg gcccgacgcg atggcttcga tgacggtcgc cggccctgtt accgcatcgc
     1681 cgcaggcaaa tatgccttcc tggccggtcc attgcgaatc cccttgcacc ttcatgggaa
     1741 gccccgcaga cccgaagaag ccggcatcag gcgtttcgcc caccgcgagg atcacacaat
     1801 ccgcggcgat ctcaaactcc gagtccttta cgggatgcgc cgtccggcgg ccgctttcat
     1861 ccggcgcgcc gagctccgtc ctcacgcatc tcaaacccgt cgccttcccg tccttccctg
     1921 tcacctcaac cggtgccgac aggaagacga tcttcacgcc ctcggtctca gccgcctcga
     1981 tctcctccgg gtacgcgggc atctcatctt tcgatctccg gtagaatatg gtgacctctt
     2041 tcgctcccat gcggtggacc gtccttgccg catcgacggc aacgttgcca ccgccgacga
     2101 ccgcgacctt ttccccgatg gtcacagaga ctcccaggtt caccgccttc aggaaaccca
     2161 caccatgatg cacaccctcg agcccttcgc cctcgatccc ggggctcacg ctcctgtggg
     2221 ctcctatggc gacaacgatc gccttgaacc cttcctcgcg caggctgccg aaaggcatgc
     2281 cgccttcacc gacaggggta ttgagccgta tctccacgcc gcgtttttcg atggcatcga
     2341 tctcttccct taccatcttc ttcggcaggc gatactcggg aatgccgagc gccagcatgc
     2401 cgccgggtac gggggccgct tcaaagaccg taacggggta accgagcttc gcgagctcga
     2461 aggcggcggt catgcccgcc ggtcccgaac cgatcacggc aaccctttca tcccgcgtcc
     2521 tcggaaaagg ctcgaccttc tccctttttc ccgctttcgc cacggcatcc acgacaaacc
     2581 gtttcaaccc gttgatcgcg atcggtcggt cgaacgtgcc cctgttgcat actctctcac
     2641 acggatgcgg gcacacccgg ctcaccgatg cgggaaaggg gatcttctct tcgatcaccg
     2701 ccagggcctt ttcgtactct ccctgtgcag ccagatcgat gtagctccgg atatccaatc
     2761 ctgccgggca ggcggccttg catggaggcg caaggagcca cggagatgga gcggacaggg
     2821 cgcccttggg ccattcgatg cagacgacgg tttcatacct ttcgtccgcc tcgctgcagt
     2881 acccgcaggc ggcgcatttc acgatctcgt cagccctgcc ttctctcgcc ttcacgggcc
     2941 agtcagggtc ggcaaaaagg gtcctcgcca tgcctatgac atccgacctt cc
//
LOCUS       DAOQTR010000269         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38660_length_2985_cov_3.082935, whole genome shotgun sequence.
ACCESSION   DAOQTR010000269 DAOQTR010000000
VERSION     DAOQTR010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38660_length_2985_cov_3.082935"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PKN85_07120"
     CDS             <1..355
                     /locus_tag="PKN85_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNT44217.1"
                     /translation="SVHGRTLTALYNKYVDAGFDPLMPYASGGLEVSRKAGGFTIGKV
                     REERKAQADPDSAAWTMPDLGGLSMKAALDRISTKGQAVRIYGSGVVTDQYPKPRERV
                     EKDTRCILYGRTYQK"
     gene            352..1803
                     /locus_tag="PKN85_07125"
     CDS             352..1803
                     /locus_tag="PKN85_07125"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNT44218.1"
                     /translation="MRLSELIRGMKKTAVTGDTSLEITGVTKDSRNVSEGSLFFATST
                     SSAYLNDALERGARAVVTDGDIDRTFACVIRVEDVKRALGEAASKFHGHPSRKMHVTG
                     ITGTNGKTTTTYLIESIFEAAGKRPGVIGTISYRYAGKTLNAPNTTPGASETNALLRD
                     MMDAGISHVIMEVSSHALHQSRVEGIDFDVAVFTNLTHDHLDYHGDFESYKASKRLLF
                     EDFLARSRKKRRYAIMNIDDPSIRDFAVAPPVTMLSYSTRGFADACLSSYSESIDGLT
                     LTLSLPGKKIPIVSPLVGMFNASNILAASLSGHVTGISPQAIKEGIERLGGVPGRLER
                     VANDRGISIFVDYAHTPDALENVLSLLGRLKKGRLIVVFGCGGNRDAVKRPLMGAIAA
                     RFADEVIVTSDNPRNEDPLTIIEDIRRGLEGKAARIIPDRREAIFEGIGSARPDDVVL
                     VAGKGHEDYQIIGGTTLHFSDREVIEESLHVAT"
     gene            1790..>2985
                     /gene="murF"
                     /locus_tag="PKN85_07130"
     CDS             1790..>2985
                     /gene="murF"
                     /locus_tag="PKN85_07130"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNT44219.1"
                     /translation="MWRLEDIVKAVGGTVLKKEKEVFAAISTDSRTIGKGDLFIPILG
                     KSFDGHAFIGEALKRSGGGTLCRADSPVSLADADGTVILVEDTNQALLDLARFKRHTL
                     SGAFISITGSNGKTTTKEILAAMMTKRYGVHFNEKNLNNLIGVPMSILSVAGDPEICI
                     LELGTNTPGEIKKLAVATNPDASLITNVNPSHLEGLASLEGILEEKLDLFRHTKEGGK
                     IFVNIDDPGIAGWWRTTQRTTVTFGIDSDADFRLAVGEDLGWEGSDVIISCPAGTIRA
                     RTSLLGRHNLYNILAAAAIASTMGVEGELIGEAIGKFVSYDKRFQPATSPKGYVIIDD
                     TYNANPSSMKWAISTLAALPAAGKRVAVLGGMKELGEMSARYHRELGAYLREAGLGLI
                     VLLGEET"
BASE COUNT          678 a          925 c          889 g          493 t
ORIGIN      
        1 cagcgtgcac ggcaggacgc tcacggcgct ctacaacaag tatgtcgatg ccggcttcga
       61 ccccctcatg ccctacgcca gcgggggcct tgaggtctcg cggaaggccg gcgggttcac
      121 gataggaaag gtccgcgagg aaagaaaggc gcaggccgac ccggacagcg ccgcatggac
      181 gatgccggac ctcggtggtc tttcgatgaa ggccgccctc gacaggatat cgacgaaagg
      241 acaggcggta cgcatctacg ggtccggcgt cgtcaccgac cagtacccca aaccgcggga
      301 gcgggtcgag aaggacaccc ggtgcattct ctacgggagg acataccaga agtgagattg
      361 agcgagctca tacggggcat gaagaagacc gccgtgaccg gtgacacgtc cctcgagata
      421 acgggcgtca cgaaagattc ccggaacgtc tccgaggggt ctctcttttt cgctacctcc
      481 acgagcagcg catacctcaa tgacgctctg gaacgggggg cgcgcgccgt ggtcaccgac
      541 ggagacatcg accgtacctt cgcgtgcgtc atccgcgtcg aggatgtgaa gcgggcactc
      601 ggcgaggccg cgtcgaagtt ccacggccac ccctcccgca agatgcatgt cacgggcatc
      661 acggggacca acggcaagac caccacgacc tatctgatcg agtccatatt cgaggcggcg
      721 ggaaaaagac ccggcgtcat cggcaccata tcctaccgct acgccggcaa gacactcaat
      781 gcgcccaaca caactcccgg cgccagcgag acgaacgccc ttcttcgcga catgatggac
      841 gccgggataa gccacgtgat catggaggtc tcctctcacg cgctccatca gtcacgcgtt
      901 gaaggtatcg acttcgatgt cgccgtattc accaacctca cccacgacca tctcgattat
      961 cacggcgact tcgaaagcta caaggcctca aaaaggcttc tcttcgaaga ttttctcgca
     1021 aggagcagga agaagagaag atatgccata atgaatatcg acgatccctc catacgggac
     1081 ttcgccgtcg ccccaccggt gacgatgctg tcctacagca cgcggggttt cgctgatgcc
     1141 tgcctgtcga gctattccga gagcatcgac ggcctgacac tcaccttatc cctaccgggg
     1201 aagaagatcc ccattgtctc acccttagtc ggcatgttca atgcctccaa catccttgcc
     1261 gcatcattgt caggccatgt cacaggtata tcgccccagg cgataaagga agggatcgaa
     1321 cggctggggg gcgtcccggg aagactggag cgagtggcga acgacagagg catttccatc
     1381 tttgtcgatt acgcccacac cccggacgcg ctcgaaaatg tcctcagtct gctcggaagg
     1441 ctcaagaaag ggaggctcat cgtggtcttc ggttgcgggg gcaacaggga tgccgtgaag
     1501 agacccctca tgggcgccat cgccgcgcgc ttcgccgacg aggtgatcgt cacctccgac
     1561 aacccgcgca acgaggaccc gctcacgatc atcgaggaca tcagacgggg cctcgaaggc
     1621 aaggcggcga ggatcatccc ggacaggcgc gaggccatct ttgaaggcat cgggtcggcg
     1681 agacccgacg acgtcgtgct tgtcgcaggc aagggacatg aggattacca gatcatcggc
     1741 ggaaccaccc ttcacttcag cgaccgcgaa gtaatcgagg agtctctcca tgtggcgact
     1801 tgaggatatc gtgaaggcgg tgggaggaac ggtcctcaaa aaggaaaagg aggtgttcgc
     1861 ggccatctcg acggattccc ggacgatcgg gaaaggggac ctcttcatcc ccattctggg
     1921 caagtccttt gacggtcacg cgttcatcgg ggaggccctc aaaaggtcgg gagggggaac
     1981 gctgtgccgc gccgacagcc ccgtgtccct tgcggatgcc gacgggaccg tcatcctagt
     2041 ggaggacacc aatcaggcgc tcctcgacct ggcccgcttc aagaggcaca ccctttcggg
     2101 cgccttcatc tcgatcacgg gaagcaacgg caagacgacg acgaaggaga tcctcgccgc
     2161 catgatgacg aaacgctacg gcgtccactt caacgaaaag aacctgaaca acctcatcgg
     2221 tgtgccgatg agcatcctct ccgttgccgg ggaccccgag atatgcatcc tggagctggg
     2281 cacgaacacg cccggcgaga taaaaaagct cgccgtggca acgaaccccg atgcatccct
     2341 catcacgaac gtgaacccct cccatcttga agggctcgcg tcgctcgagg gcatcctcga
     2401 ggaaaagctc gatctcttcc ggcacacgaa ggaaggcggg aagatattcg tcaacatcga
     2461 cgatcccggc atagccggct ggtggcgaac aacacagagg acaaccgtca ccttcggcat
     2521 cgacagcgac gccgacttcc gtctcgcagt cggggaagac ctcggatggg aaggctccga
     2581 tgtcatcatc agctgcccgg cggggaccat acgggcgcgg acttctctcc tcggcaggca
     2641 caacctctac aacatccttg ccgccgccgc gatagcgtcg accatggggg tcgaaggcga
     2701 gctcatcggg gaggccatcg ggaagttcgt ttcctacgac aagagattcc agcccgcgac
     2761 aagcccaaag ggctacgtca tcatcgacga tacgtacaac gcgaacccct cgtctatgaa
     2821 gtgggccata agcacccttg cagctcttcc cgcggcaggg aaaagggtgg ccgtcctcgg
     2881 gggcatgaag gaactcggcg aaatgagcgc gcggtaccac agggagctag gggcatactt
     2941 gcgggaagca gggctcgggc tcatcgtcct tctcggtgaa gagac
//
LOCUS       DAOQTR010000270         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_38915_length_2971_cov_2.900892, whole genome shotgun sequence.
ACCESSION   DAOQTR010000270 DAOQTR010000000
VERSION     DAOQTR010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38915_length_2971_cov_2.900892"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /gene="sucC"
                     /locus_tag="PKN85_07135"
     CDS             complement(<1..1217)
                     /gene="sucC"
                     /locus_tag="PKN85_07135"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNT44220.1"
                     /translation="MKIHEFQAKKLLSEYGVPIPRGQIARTDEEALNVARQLGEGPFV
                     LKAQVHAGGRGKAGGIRVVDNIDDVGRAAWEMLGKTLVTAQTDEEGKPVRTLLVEEAV
                     NVGKEVYIGIVIDRDKACPVILASPEGGVEIERLARERPDRVITEVVDPSVGFRPFQA
                     NRIFYGLGLDESLARPVMASVIGLYRLLVEKDLSLAEINPLVVTDEGGIIALDAKVVA
                     DDNALFRHPDIAGLRDVRQEDPLEVEAGKHNLNYIKLKGNVGCMVNGAGLAMATMDLI
                     KLAGAEPANFLDVGGGATSRMVKEAMRILVFDKDVKMIFINIFGGILRCDTLAQGVVE
                     AARELVIDVPIVVRLEGTNVEEGRRMLADSGLSFAVASDLADAARMVGETVRGIGPIL
                     LFVLFPSLSPPPFS"
     gene            complement(1204..1791)
                     /locus_tag="PKN85_07140"
     CDS             complement(1204..1791)
                     /locus_tag="PKN85_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT44221.1"
                     /translation="MAFRYDIRLSGSGGQGIILMGIILAEAIGIYDGMFVAQTQSYGP
                     EARGGSSKAEVVVSDGEIDYPKALQLDLLVAMNQKSCDDYYMDLKPDGILVVDSTLVR
                     QVPLSRAYQIPFTRIAREELKREMVANIVALGALARLTPIVSTRAIESAVLGRVPKGT
                     EKLNRDALQAGMRAARKTKKVRFPEKISELVDEDS"
     gene            complement(1793..2629)
                     /locus_tag="PKN85_07145"
     CDS             complement(1793..2629)
                     /locus_tag="PKN85_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNT44222.1"
                     /translation="MSDITKLIHHYLRHDKKFPHVWCPGCGIGIMLGALIRAIDHKGY
                     EKDEIVLVSGIGCTGRLPVYVDFNTLHTTHGRALTFATGIKLAKPRLKVIVVMGDGDA
                     VAIGGNHFIHAARRNIDLTAVIVNNSVYGMTGGQYSPTTPYGMKTATSVYSNVEHSFR
                     ISELAVTAGAVFVGRGTVYHAKLLESLMEKAFQKVGFSVVEIISHCHTQYGRMNRMGS
                     AVEMMQWQRDHAVTMEKAAQMNEEDLKDKFRIGVLIDRDLPPYQDEYEKVRRWSKGAE
                     RK"
     gene            complement(2629..>2971)
                     /locus_tag="PKN85_07150"
     CDS             complement(2629..>2971)
                     /locus_tag="PKN85_07150"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNT44223.1"
                     /translation="QIGEHFHIDDADVAIIAYGCVARSAKRAVSDCREKGIKAGMLKL
                     NTIWPFMRTDVEKVLRRAKVVIVPEMNMGQYSREVKRVNKGEAKVFTINRVDGTIITP
                     QEIMARIKEVF"
BASE COUNT          529 a          965 c          804 g          673 t
ORIGIN      
        1 gagaatggtg ggggagacag tgaggggaat aggacgaata ggaggatagg tcctattccc
       61 ctcactgtct cccccaccat tctcgccgcg tccgcgaggt cggatgccac cgcaaagctg
      121 aggcccgaat cggcgagcat cctcctgccc tcttccacgt tcgttccttc aaggcggacg
      181 acgatgggaa cgtctatcac cagttcgcgg gccgcttcga caacaccctg cgccagtgtg
      241 tcacaccgga gtatcccgcc gaagatgttg atgaatatca tcttcacatc cttgtcgaaa
      301 acgaggatcc tcatggcctc ctttaccatt cgcgacgtcg cccctccgcc gacatccagg
      361 aagttggcgg gttccgcgcc ggccagcttg atgaggtcca tcgttgccat ggcaaggcct
      421 gccccgttta ccatgcagcc cacattcccc ttgagcttta tatagttgag gttatgtttc
      481 cccgcttcca cctcgagcgg atcttcctgt cttacgtccc tcaggcccgc gatgtcggga
      541 tgccggaata gggcgttgtc atcggcgacg acctttgcgt cgagggcaat tatgcctcct
      601 tcatccgtca caacgagggg attgatctcg gcgagggaaa ggtccttctc gacaaggagt
      661 ctgtacagtc ctatcacgga ggccatcacc ggccgggcaa gggattcgtc gagaccaagc
      721 ccgtagaata tcctgttcgc ctggaagggt cgaaatccca cggaggggtc gacgacctcg
      781 gtgatcaccc ggtccggtcg ctcccgggca agtctttcga tctccacacc cccttccgga
      841 ctcgccagta tcacggggca ggctttatcc ctgtcgatga cgatgccgat atagacctcc
      901 ttgccgacgt tcaccgcctc ttcgacgagg agtgtccgga ccggttttcc ctcctcatcc
      961 gtctgagcgg taaccagggt ctttccgagc atttcccacg ccgcgcggcc gacgtcgtca
     1021 atgttgtcca caaccctgat gcccccggcc tttccccgtc cgcccgcgtg gacctgcgct
     1081 tttaggacaa acggcccctc gccgagctgc ctggcgacat tcaacgcctc ttcgtctgtt
     1141 ctcgctatct gcccccgcgg aatgggaaca ccgtactccg aaagaagctt tttcgcctga
     1201 aactcatgaa tcttcatcga caagctccga gatcttctcg ggaaaccgca cctttttcgt
     1261 cttccgggcc gccctcatcc ccgcctggag agcatccctg ttgagcttct ccgtcccctt
     1321 ggggacgcgg ccgaggacgg cgctttcaat ggcccgggtg gagactatgg gggtaagccg
     1381 cgccagggcc cccagagcga cgatattggc gaccatctct cttttcagtt cttcccgcgc
     1441 tatgcgtgta aaaggtatct ggtacgccct cgacaggggt acctgcctga ccagggtcga
     1501 atcaacgacg aggatcccgt caggtttgag gtccatgtag taatcatcgc aggacttctg
     1561 gttcatggcc acgaggaggt ccagttggag ggccttgggg taatcgatct cgccgtcgct
     1621 gacaacgacc tcggccttgc tcgaaccgcc gcgcgcctcg gggccgtagc tctgcgtctg
     1681 cgccacgaac atgccgtcgt agatgcctat ggcctcggcg aggatgatgc ccatgagaat
     1741 gatcccctgc cctccggagc cgctgagcct gatatcgtac cgaaaggcca tgctatttcc
     1801 tctccgcgcc cttcgaccac ctccgtacct tttcgtactc atcctggtac gggggcaggt
     1861 ccctgtcgat aaggacgcct atcctgaact tgtctttcaa gtcttcctca ttcatctgcg
     1921 cggccttctc catcgtcacc gcatggtccc tctgccattg catcatttcc acggcgcttc
     1981 ccatccggtt cattcgcccg tactgagtgt ggcagtggga tatgatctcc acaaccgaga
     2041 accccacctt ctggaacgcc ttttccatga gactctcgag cagcttggcg tgatagaccg
     2101 ttccccggcc gacgaagacg gcccccgccg tcacggcgag ctcggatatt ctgaaggaat
     2161 gctccacgtt ggaatagacg ctcgtcgccg tcttcatacc gtagggcgtc gtcggggagt
     2221 actgccctcc cgtcatgccg tagacgctgt tgttgacgat gaccgccgtg agatcgatgt
     2281 tgcggcgggc cgcatggatg aaatggttgc cgccgatggc caccgcgtcg ccgtcaccca
     2341 tcaccacgat caccttgagg cggggctttg ccagctttat ccccgtcgcg aaggtaagcg
     2401 ccctgccgtg ggtggtatgc agggtgttga aatcgacgta cacgggaagt ctcccggtgc
     2461 accctatgcc cgagaccagg acgatctcgt ccttttcgta ccccttgtgg tcgatggccc
     2521 tgataagggc gccaagcatg atgcctatac cgcatccggg gcaccacacg tggggaaact
     2581 tcttgtcgtg gcggagataa tgatggatca gtttggtgat gtcgctcatc agaacacctc
     2641 cttgatccgg gccattatct cctgaggtgt tatgatggtg ccgtccaccc tgttgatggt
     2701 gaagaccttg gcctcgccct tgttcacccg cttcacctcc cgcgagtact gtcccatgtt
     2761 catctcgggc acgatgacca ccttcgcccg cctgagcacc ttctccacgt cggtcctcat
     2821 gaacggccat atcgtgttca gtttgagcat tccagccttt atccctttct cccggcagtc
     2881 cgacacggca cgcttcgccg agcgggcgac gcacccgtag gcgatgatcg caacgtcggc
     2941 atcatcgata tggaagtgtt cgccgatctg c
//
LOCUS       DAOQTR010000271         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39017_length_2965_cov_4.347079, whole genome shotgun sequence.
ACCESSION   DAOQTR010000271 DAOQTR010000000
VERSION     DAOQTR010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39017_length_2965_cov_4.347079"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKN85_07155"
     CDS             complement(<1..810)
                     /locus_tag="PKN85_07155"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT44224.1"
                     /translation="MKWFNNWSIGTKVMSISLFTIVIIMAGMILYFIPMVKDKLMGEK
                     KTALSDHTDIAYTIINSYEAKVKSGEFKVDDAKQRGIANLKSVRYSGNEYFFIMDTSG
                     KVIMHPINAQLDGKDVSNEKDPEGKYFFREMAQTAKEKGEGFVAYMWPKAGNSKPVPK
                     ITFVKLFKQWDWVIGTGIYVDDVEAETSKIQWQVIIATVVCAIVILCIAFFVSRKVKN
                     ALNDAVHVANDLAEGNLTVDVEIRSEDETGRLLRAMKNMVEKLREVVEGVSS"
     gene            1037..1639
                     /locus_tag="PKN85_07160"
     CDS             1037..1639
                     /locus_tag="PKN85_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNT44225.1"
                     /translation="MDLTREQLEMVFAVLTGIGLSAACGFRIFLPLLALNLASMYGLV
                     GLAPGFEWIAGYQVTMAFGIATVLEIVAYYIPWLDNIMDSIASPVSVIAGTIATASLI
                     TDIPSSLKWTISIVAGGGIAGLLQGATVALRAKSTIFTAGLGNFLVASFELLASAIVA
                     FLSLILPIAAFILALLLITYCLILARRVYMKKKRGRGEAA"
     gene            complement(1803..>2965)
                     /locus_tag="PKN85_07165"
     CDS             complement(1803..>2965)
                     /locus_tag="PKN85_07165"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HNT44226.1"
                     /translation="GIVYCMTRKKTEEIARFLREKGLPALPYHAGLDRDVRLRHQREF
                     LEGDSTIMVATIAFGMGINKPDIRYVAHLNLPRTLEGYYQETGRAGRDGEPADAWMVY
                     SLADIVMLRQMLDASPGDEDFKAIQYRKMAAMLGYSETARCRRQVLLGYFGETLAETC
                     GNCDTCLGNVEVVDGTLLAQKALSCAYRTGQMFGVNHLVDVLVGKDTARIRAFGHNSV
                     STFGIGGELSAREWRSVYRQLVAAGLMKVDMEKKGGLRLSGKCHPVLRGEEVFELRKD
                     PSPSARTTGKRTLKGAGAETPNVEADGLAEGFAFDPDLWERLRSLRGELARSSSLPAY
                     TVFHNNTLREMVTRLPQTLEEMLAISGVGQAKLEKYGEIFLEAIKKHKEETA"
BASE COUNT          528 a          883 c          842 g          712 t
ORIGIN      
        1 ggagctgaca ccctccacga cctcgcggag cttctccacc atgttcttca tggcccgaag
       61 gagcctgccc gtctcgtcct cgctccgtat ctccacgtcg acggtgaggt tgccctcggc
      121 caggtcgttg gccacatgga cggcgtcgtt caacgcgttc ttcaccttgc gggagacgaa
      181 gaaggcgatg cagaggatga cgatggcaca gacgacggtg gctatgatga cctgccactg
      241 gatcttggag gtctccgctt cgacgtcatc gacatagata cccgtgccga tgacccagtc
      301 ccactgtttg aaaagcttca cgaaggtgat cttgggaacg ggtttggagt tgcctgcttt
      361 gggccacatg taagccacga agccttcccc cttctccttg gcggtctggg ccatctcgcg
      421 gaagaagtac ttgccctccg ggtccttctc gttcgacacg tccttcccgt cgagctgggc
      481 gttgatagga tgcatgatta ccttgccgct cgtgtccatg atgaagaagt actcgttccc
      541 ggagtagcgg acggacttca ggtttgcgat accccgctgt tttgcatcgt cgaccttgaa
      601 ctcgccggac ttgaccttcg cctcgtaaga attgatgatc gtgtaggcga tatcggtgtg
      661 atcgctcaat gccgtcttct tctcgcccat cagcttgtcc tttaccatgg ggatgaagta
      721 gaggatcatc ccggccatga tgatgacgat ggtgaagagg gatatgctca tcacttttgt
      781 tccgatggac cagttgttaa accatttcat aataacctcc ttgtatggat gcaggactgt
      841 gctgctcttt accgtgatgg attacctgag cctgccggct attctgctgt gaaggtgaac
      901 tctccaacat atcatcagtt gtcggtattt tgatgcgggg gaatatggcc tattgtctta
      961 ttttgttgta agacgagtcc tacacggaag cattctcttc ttgaccctgg tatccgggaa
     1021 ggacgaaaat taaggtatgg atctgacgcg ggaacagctt gaaatggtat tcgcggtcct
     1081 tacggggatc ggcctcagcg cggcctgcgg gttcaggatc tttctgcccc ttctcgccct
     1141 gaaccttgcc tccatgtatg gcctcgtcgg cctggcgccg ggattcgaat ggatcgcggg
     1201 gtaccaggtg acgatggcct tcgggattgc cacggtgctc gagatcgttg cctattacat
     1261 tccctggctt gacaacatca tggactccat cgcctcgccg gtgagcgtca tcgcgggcac
     1321 gatcgcgacg gcatcgctca taacggacat cccctcttcg ctgaagtgga ccatttcgat
     1381 cgttgccggg ggcgggatag cgggcctgct tcagggcgcc acggtggccc tgcgagccaa
     1441 atccaccatc ttcaccgccg ggctgggcaa tttcctggtc gccagcttcg agctccttgc
     1501 gtccgccatc gtcgccttcc tgtcgctcat cctccccatc gccgccttca tcctcgctct
     1561 gctcctcatc acctactgcc tcatcctggc ccggagggtg tatatgaaga agaagagggg
     1621 caggggagag gcggcttgaa cagcttgagc cgaaaaagat tgttgcaacc gcttgaaccg
     1681 cttgaacgtt aaaagaaaaa gacaaaagag cacggtccgg ctcccagttg tcaccccggg
     1741 ctccgggcag tgtcattgtc ctttgctttc cccgttcaag cggctcaagc tgttcaagcg
     1801 gttcaagctg tctcttcctt atgtttcttt atggcttcga gaaagatctc cccgtacttt
     1861 tccagttttg cctggccgac tcctgagatg gccagcatct cctccagtgt ctgcggcagg
     1921 cgtgtcacca tctctctgag ggtgttgttg tgaaatacgg tgtacgcggg gagagaggag
     1981 cttctggcaa gttctcctcg cagtgagcgg agacgttccc agaggtcggg gtcaaaggcg
     2041 aagccctccg caaggccatc agcttcgacg tttggggtct ctgccccggc gcccttgagg
     2101 gtcctcttcc ctgtggtcct cgcggaaggt gagggatcct tgcggagctc gaacacctct
     2161 tctcctcgca gcacggggtg gcatttgccc gacagacgca gccccccctt cttttccatg
     2221 tccaccttca tgagccccgc ggcgacgagc tgcctgtaca cggacctcca ttcacgtgcc
     2281 gacagctccc cgcctatgcc gaatgtcgac acgctgttgt ggccaaaggc ccgtatgcgt
     2341 gccgtgtcct ttccgactag tacgtcgacg agatggttga ccccgaacat ctgacctgtc
     2401 ctgtaggcgc aggagagtgc cttctgcgcg agcagggtgc cgtccacgac ctccacattc
     2461 ccgaggcatg tgtcgcagtt gccgcaggtt tccgcaaggg tctctccaaa gtacccgagg
     2521 agcacctgcc gtcggcagcg agcggtctcg gagtagccga gcatcgctgc catcttgcgg
     2581 tactggattg ccttgaagtc ttcatctccc ggggaggcgt cgagcatctg ccggagcatg
     2641 acgatgtcgg ccaggctgta gaccatccac gcgtccgccg gttctccgtc acgtcccgcg
     2701 cgccctgttt cctggtaata gccttccagg gtccggggaa ggttgaggtg ggcgacatag
     2761 cgtatgtcgg gcttgttgat gcccatgccg aaggctatgg tcgcgaccat gatggtgctg
     2821 tccccttcaa ggaattccct ctggtgcctg agccgcacgt cccgatcgag acccgcatgg
     2881 tagggcaggg cgggaagccc cttctcccga aggaatcgcg cgatctcttc agtcttcttc
     2941 cgtgtcatgc aatagacgat gcccg
//
LOCUS       DAOQTR010000272         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39184_length_2957_cov_3.110958, whole genome shotgun sequence.
ACCESSION   DAOQTR010000272 DAOQTR010000000
VERSION     DAOQTR010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39184_length_2957_cov_3.110958"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKN85_07170"
     CDS             <1..307
                     /locus_tag="PKN85_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44227.1"
                     /translation="MLVSSVISGSSNAFLTLRVGAITKQYCGSLVMPTKRTVRRQATL
                     EATRMLGSIVAEGTKKVYDAIWTSSKSRLGTAIDDIGAYAKRIWMKLGGEGPKSAEP"
     gene            complement(304..684)
                     /locus_tag="PKN85_07175"
     CDS             complement(304..684)
                     /locus_tag="PKN85_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNT44228.1"
                     /translation="MAYTASTASNKDEKAGTVFAGDLEVQGTVKFKSSLMIEGIFSGE
                     IISEGVLTVGPSAKVTATIRTKTLVSRGEINGDVYADEQVVLEETAVHNGNITSPGIV
                     IERGSTFNGSCSMKRADTGNTGNF"
     gene            complement(871..1146)
                     /locus_tag="PKN85_07180"
     CDS             complement(871..1146)
                     /locus_tag="PKN85_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HNT44229.1"
                     /translation="MANDPGFGYRVTATVVETKGSCNAGHKAGDTFELSCHNPAGLCG
                     FFYHSIFGDLEAFQFGGSLPWWDGDKIEVQCPDVANMVTLELERKKR"
     gene            complement(1327..2373)
                     /locus_tag="PKN85_07185"
     CDS             complement(1327..2373)
                     /locus_tag="PKN85_07185"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNT44230.1"
                     /translation="MYISVIGPALSGKTTLFQALSGVESARGFSDGDNIISIDVPDER
                     VDELSRIYRPKKTTYARIELADTVAISEGNVKDSTINAKTLQKMRMSDAFILTLNDFE
                     EAAAAELEKEFHGIMSEFILADMILIEGRLERIKKQAGKKENTALLLEEKLLEQCLAH
                     VGEGKPLRTLELAVSDEKTLRGFRFLSQKPMMIAINCSEDGMGEMPGEEERDMEGCPV
                     IHVCASLECELAMMEEAERAAFMEEFSVKESIRGRMIRLAFDTLGLISFLTVGEDECR
                     AWPIRKGMNAQEAAGAIHTDLASKFIRAETVSYADLMKYGGMAECKKAGVWRLEGKTY
                     IVEDGDIISIRAGT"
     gene            complement(2573..>2957)
                     /locus_tag="PKN85_07190"
     CDS             complement(2573..>2957)
                     /locus_tag="PKN85_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44231.1"
                     /translation="DRIDIHIEVPPVTIAELSRERPEEPSAAIRERVSKARAIQLDRF
                     NGRNIHANAQMSARVIRKHCPLDTPTRQLLEAAVDKWVLSPRAYHRILKVSRTIADLD
                     ASTDIAEAHVAEAIRYRALDKRLTI"
BASE COUNT          561 a          878 c          820 g          698 t
ORIGIN      
        1 gatgcttgta agctccgtca tatcggggtc gtccaatgcc ttcctcacac ttagggtggg
       61 ggcgataacg aaacagtatt gcgggtctct cgtcatgccc acgaagcgga cggtccgccg
      121 ccaggccacc ctggaggcga caagaatgct gggttccatc gtggccgagg gaacgaagaa
      181 ggtatacgac gcgatctgga cctcgtcgaa gagcaggtta gggacagcta tcgacgatat
      241 aggcgcctac gcgaagagga tctggatgaa actgggcggc gagggaccga agagcgccga
      301 accctagaag ttgcccgtgt tccccgtgtc cgccctcttc atggaacagg acccgttgaa
      361 ggtcgaccct ctttctatga cgatgccggg agaggttata ttgccgttgt ggacggccgt
      421 ctcttcaagc acgacctgct cgtcggcgta aacgtctccg ttgatctcgc cgcgggacac
      481 gagggtcttt gtccgtatcg tggcggtgac cttagcggat ggccccaccg tcaggacgcc
      541 ttcggagatg atctcgcccg agaagatgcc ttcgatcatg agcgaactct tgaactttac
      601 agtcccctgg acctcgaggt cgccggcaaa caccgttccg gccttttcat ccttgtttga
      661 agcagttgaa gctgtgtacg ccattctttc ctcttttagt tcacgtattt tctactctat
      721 ttccccctgc tggacgggaa ggttgtacat tgtgagcata tatcttacca gatgttgccg
      781 gaaaacgcga aattaaatct ccaccaccct gaagacggtt caagagttca agggttcaaa
      841 ggctttccct cttgaaccct tgaaccgtct ttatctcttt ttcctttcca gttccagcgt
      901 gaccatgttc gccacgtcgg ggcattggac ctctattttg tcgccgtccc accaggggag
      961 gctgccgccg aactggaatg cctcgaggtc gccgaagatg ctgtgataaa aaaagccgca
     1021 caaccccgcg ggattgtgac agctcaactc aaaggtatca cccgctttgt gaccggcatt
     1081 gcagcttccc ttcgtctcaa cgaccgtcgc cgttaccctg tacccgaagc caggatcatt
     1141 tgccatgtga attgacctcc tttctcatag tatgacctgc cccattccat cggaacgcct
     1201 tattacctat catacagata ttccggaaat acgcaacggt tggtgagcgc ccgcggaaag
     1261 agacgttccg ccaaagggga tgacgaccga tcaccaacgg agaagataat gaccattccg
     1321 gggtcctcag gtcccggccc ttattgagat gatgtcccca tcctcaacga tgtacgtctt
     1381 gccttcgagc cgccagacgc cggccttctt gcattcagcc ataccgccgt acttcatgag
     1441 atcggcgtag gagacggtct cggcacggat gaacttcgag gccaggtcgg tatggatggc
     1501 ccccgccgct tcctgcgcgt tcatgccctt ccggatcggc catgcccggc attcgtcctc
     1561 tcccactgtt aggaacgaga taagtcccaa cgtgtcgaag gcgaggcgta tcatgcggcc
     1621 ccttatggac tccttgacgg agaactcctc catgaaggcg gcgcgttcag cctcctccat
     1681 catggcaagc tcgcactcca gactggcgca gacgtggata acggggcagc cctccatgtc
     1741 ccgctcctcc tccccgggca tctcgcccat cccgtcctcg gagcagttga tggcgatcat
     1801 catgggtttc tgggagagga acctgaaacc cctgagcgtc ttctcatcgc tcactgcaag
     1861 ctcgagcgtc ctcaacggtt tgccctcccc cacgtgggcg aggcattgct cgaggagttt
     1921 ttcctccagg agaagggccg tgttctcctt cttcccggcc tgtttcttga tgcgctcgag
     1981 gcgtccttcg atgaggatca tgtcggccag tatgaactcg ctcattatgc cgtggaattc
     2041 cttctcgagc tccgctgcag cggcctcctc aaaatcgttg agtgtgagga tgaaggcatc
     2101 gctcatgcgc atcttctgca gcgtcttcgc gttgatggtg gagtccttga cgttgccctc
     2161 cgatatcgcg acggtgtcgg caagttctat ccgcgcgtag gtggtcttct tcggtctgta
     2221 gatcctcgac agctcgtcga cgcgctcatc aggtacatcg atggagatga tgttgtcccc
     2281 gtcggaaaac ccccgcgcgc tctccacgcc gctcagcgcc tggaaaagcg ttgtcttgcc
     2341 ggagagagcc ggcccaatga cggaaatata catcctcttc tcccctgtct catatctcag
     2401 gaagccatcg cggcccgacc atcggctttc atgcgattgt accgctgacc tcgaacgccc
     2461 gtaatgccag cctgggccac cattatagct ccttttcccc tgacgttcaa gccgctcaag
     2521 cggttcaagc cattcaagtg gttcaagcca ttcaagtggt tcaagccgcc tttcaaatgg
     2581 tcagtctctt gtcgagcgca cggtatctga tcgcttccgc tacatgcgcc tcggctatgt
     2641 cggtgcttgc gtcgaggtcg gctattgtgc gcgatacctt gagaatgcgg tggtaggcac
     2701 ggggggagag gacccatttg tccacggcgg cttcgagcaa ttggcgggtc ggggtgtcga
     2761 ggggacagtg tttgcgtatc acccgggcgg acatttgcgc gttggcgtgg atgttccttc
     2821 cgttgaaacg gtcgagctgt atggcgcggg ctttggacac cctttcgcgg atggcggcgg
     2881 agggttcttc cggtcgctcg cgggaaagct cggctatggt tacgggaggc acctcgatgt
     2941 ggatgtcgat ccggtcc
//
LOCUS       DAOQTR010000273         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39211_length_2956_cov_2.609790, whole genome shotgun sequence.
ACCESSION   DAOQTR010000273 DAOQTR010000000
VERSION     DAOQTR010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39211_length_2956_cov_2.609790"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKN85_07195"
     CDS             complement(<1..243)
                     /locus_tag="PKN85_07195"
                     /inference="COORDINATES: protein motif:HMM:NF020388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44232.1"
                     /translation="MDLWEANREAGMIYMQARGQVIAGMGGAIDLNFTAVKDLMQMYG
                     VMDWKRCWEKVHRCFHVFLKEQRDKQKLEQMRPHGRR"
     gene            complement(351..746)
                     /locus_tag="PKN85_07200"
     CDS             complement(351..746)
                     /locus_tag="PKN85_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44233.1"
                     /translation="MTVIDVEERTGVWFDMDGGGRVQIRTLTADDMKSIRKQTVKKRT
                     EFKKVDGTPGRFEYEEVNDELQNELFWDRVIVDWSGLYDGKGSEIPCTKENKLLLMMR
                     SIKFAKFVGECLKELSDSEAELEELASKN"
     gene            complement(756..1172)
                     /locus_tag="PKN85_07205"
     CDS             complement(756..1172)
                     /locus_tag="PKN85_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44234.1"
                     /translation="MAFLAGNDAKVALGTATVVGMGNWSLDGITVDLLETTSFGDSAK
                     QYITGLLDYGTVSFAGLYDPSDTTGQGVLISALENNSAINSIRLYVDNTSYWTPDVTT
                     NSSSAIYVTSANISIDKSGLGQVSFQARCTGPWVLE"
     gene            complement(1297..1965)
                     /locus_tag="PKN85_07210"
     CDS             complement(1297..1965)
                     /locus_tag="PKN85_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44235.1"
                     /translation="MKLTNLKLGIGVPLSYPQVPSAFFDSFIAMEKPGYFYLRTSTGP
                     IDDMRNNLVREALEIGCTHLIMMDADQIYPVDTIPRLLSHRKPVVGCLVYRRYPPFDP
                     LMLRGKINSYYTVTEWEPGELVEVDATGTGCVLFEMDVFKKMKPPWFRFRLSNGKPVG
                     EDIGFCSDLKAAGYPIHVDTGVVCGHLSNMIVNGNTWKLYRKLKEAEKAHDVQHGVVK
                     TVAS"
     gene            complement(1952..2437)
                     /locus_tag="PKN85_07215"
     CDS             complement(1952..2437)
                     /locus_tag="PKN85_07215"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT44236.1"
                     /translation="MDLMVDFVDRYDLRRKRILDMGSMDINGSYRKLFPDGEYVGADV
                     RPGKSVDVIVGSEAWDDLGTFDAVISGQTLEHVADIPQFLDDCDGKLLPGGLLCIIAP
                     SAGPRHDVPIWVGHFSKETMAGLVSDAGFEVIEAVTIKAHPFDDTRVIARKPEVVDEV
                     D"
     gene            complement(2445..>2956)
                     /locus_tag="PKN85_07220"
     CDS             complement(2445..>2956)
                     /locus_tag="PKN85_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT44237.1"
                     /translation="GKDIFARQEISSSPAPKGLCLNLGCGYRKLEGFVNIDNRPEVGP
                     DMVCDVLDGLPYEDSSVDMVRADDFLEHIPIGKTVQVVTEIWRVLKPGGIFESLTPST
                     DGRGAFQDPTHASFWNANSWLYYTDPATRHLYGMVPDFEVISIEDRLTSEALQIIHTH
                     ALLKARKDA"
BASE COUNT          613 a          926 c          754 g          663 t
ORIGIN      
        1 ccgcctcccg tgaggtctca tctgctcaag tttctgcttg tcccgctgtt ccttgagaaa
       61 tacatggaag cacctgtgaa ccttttccca acaccgcttc cagtccatga caccgtacat
      121 ctgcatcaag tctttaaccg ccgtgaagtt aaggtctatc gccccgccca tcccggctat
      181 cacctgaccc ctggcctgca tatagatcat gcccgcttcc cggttcgctt cccagaggtc
      241 caccctgcaa gtgcggcagg gcggttcctc cggtggcgtc ctactggcgt atatggtccg
      301 gcaacttggg caacggggag cctgctcgtc gctccaccgc acccactcga tcagtttttt
      361 gaggccagct cctcaagctc cgcctcgctg tcgctcagtt ccttgaggca ttccccaacg
      421 aatttcgcga acttgatgga ccgcatcatg aggagaagtt tattctcctt cgtgcagggg
      481 atttcactcc ccttcccgtc gtaaaggccg gaccaatcca cgatgacccg atcccagaac
      541 agctcgttct ggagttcatc gttgacctcc tcgtactcga agcggccagg agtgccgtcc
      601 accttcttga actcggtcct tttcttgacc gtctgcttcc gaatggactt catgtcatcg
      661 gcggtgaggg tccgaatctg aaccctcccg cctccgtcca tgtcaaacca tacgcccgtt
      721 ctctcctcta catcgataac cgtcataaat cccccttact ccagaaccca cggcccggtg
      781 catctggcct ggaaggagac ctggccgaga ccggacttgt cgatgctgat attcgccgag
      841 gtgacgtaga tcgccgaaga ggagttcgtg gtcacgtccg gggtccagta cgacgtgttg
      901 tccacataga gcctgatcga gttgatggcg ctgttgtttt ccagggccga aatgagaacc
      961 ccctgccccg tcgtgtcgga cgggtcatag agccccgcaa acgacacggt gccgtaatcc
     1021 agaagccccg tgatgtactg cttggccgag tcgccgaatg aggtcgtctc gagcagatca
     1081 acggtgatac cgtcaagaga ccagttgccc atcccgacaa ccgtagccgt gccgagtgcc
     1141 acttttgcgt cgttgccagc aagaaaagcc attttgatac ctcctaagtt ttccgggcaa
     1201 agaaaaaggg catgtcaatg tgtcggcacc gacatgccct catctttctt gcgtcccccc
     1261 ggatgtggcc gcacccggag agaacccgga ttgtttttaa ctcgctaccg ttttcaccac
     1321 cccatgttga acatcatggg ctttctccgc ttccttcaac ttccgatata acttccaggt
     1381 attgccgttc acgatcatgt ttgacagatg accgcagaca acccctgtat ccacatggat
     1441 aggatacccc gccgccttga ggtcggaaca gaaaccgatg tcctccccca cgggctttcc
     1501 gtttgataac ctgaaccgga accacggggg cttcatcttc ttaaagacat ccatctcaaa
     1561 aaggacacag cccgtcccgg tggcgtccac ctccactagt tccccaggtt cccactccgt
     1621 cacggtgtaa tagctgttga tcttcccccg gagcatcagc gggtcgaagg gcgggtaacg
     1681 gcggtaaacc aggcacccga ccacgggctt ccggtgagac aggagccggg ggatcgtatc
     1741 gacggggtat atctggtccg catccatcat gatgagatgg gtacacccga tttccaacgc
     1801 ttcccggacc aggttgttcc tcatatcgtc aatcggcccc gttgatgtcc gtaggtaaaa
     1861 gtaccccggt ttctccatgg cgatgaagga atcgaaaaag gcactcggca cctggggata
     1921 ggaaagagga acgccgatac cgagcttgag gttagtcaac ttcatcaacc acctccggct
     1981 tccgggcgat aaccctcgta tcgtcaaacg gatgggcctt gatggtcacg gcctcgatca
     2041 cctcgaaccc ggcatccgac acaagccccg ccatcgtctc cttcgagaaa tgacctaccc
     2101 atatcggaac gtcgtggcgt ggaccggcgg aaggggcgat gatgcacagg aggcctcctg
     2161 gtaacagctt gccgtcgcag tcgtccagga actgagggat gtcggcgacg tgctcaagcg
     2221 tttgaccgga aatcacggcg tcaaaggttc caaggtcgtc ccatgcctcg gaaccgacga
     2281 tgacatcgac gctctttccg gggcgaacat cggcgccgac atactcgccg tcggggaaca
     2341 gttttcggta gctcccattg atgtccatgc ttcccatgtc aagaatccgc tttcgccgaa
     2401 gatcgtagcg gtccacgaaa tcaaccatca aatccattcc agccttatgc atctttcctc
     2461 gctttcagta gggcgtgcgt atgtattatc tgaagagctt cactcgtcaa tcggtcctcg
     2521 atgctgatca cctcgaagtc ggggaccatc ccgtaaagat gccttgtcgc cgggtcggtg
     2581 tagtagagcc aagagttagc gttccagaat gaagcgtggg tcgggtcttg aaaggccccc
     2641 ctcccatctg tcgaaggggt gaggctctcg aatatccctc ccggtttcag tacccgccat
     2701 atctccgtca cgacctggac cgtcttgcct atcgggatgt gctccaagaa gtcatcggcc
     2761 cgaaccatat ccacggagct gtcctcgtag ggcaggccgt ccaatacgtc acacaccata
     2821 tccgggccca cttcggggcg gttgtcaatg ttcacaaagc cctcaagttt ccggtatccg
     2881 catccgaggt taagacacag gcccttcggg gcggggcttg acgaaatctc ctggcgggca
     2941 aatatgtcct tccccc
//
LOCUS       DAOQTR010000274         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39251_length_2954_cov_3.499483, whole genome shotgun sequence.
ACCESSION   DAOQTR010000274 DAOQTR010000000
VERSION     DAOQTR010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39251_length_2954_cov_3.499483"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PKN85_07225"
     CDS             <1..1120
                     /locus_tag="PKN85_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44238.1"
                     /translation="GSAPHAPIEPFTHMALKGEPFPLIDWDFPCFTDSQRFARIHDSD
                     RPQASTGARRRPAGPCRACAGWTACRGRFAAAAAEGGCSSFFREMAQIALTRARQAEA
                     RACLAPAAAPAPPSETENREEGEEGPTQPLQGTGGPTWIDLRGGPAQALIVSGVLKQV
                     VELHPGRRYALVARTGLTRILEKHPAIEMSGHPPKGARVLTIRAGSFPGPQEGEYQRL
                     AASFGLATPVEERLWVPWGFEDERNLMALIPRKEKNILICTSRAPHNVIPIERWEALA
                     AMLGREGMGVVQTGGRKDPSVRGAYNILGLLTERQFISLPRHFDAVVTSDALMTCAAR
                     LCRTPVVILGDSLGPDSTGLSLAAVRRAVARAVEGQQG"
     gene            complement(1138..1836)
                     /gene="modB"
                     /locus_tag="PKN85_07230"
     CDS             complement(1138..1836)
                     /gene="modB"
                     /locus_tag="PKN85_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNT44239.1"
                     /translation="MPHTLATDYLTHPAIALTLKVTLVALILQLAAGLPLGLYLAGKK
                     SPAKTVVEIITTLPMIFPPMALGFFLLLVLGKNGPLGHLLMEVFAFKIIFTPSGVLVA
                     VFVVGLPFMVKSVQAARQQMDGTLIEAAATLGKTRAQTLTKVVLPNIKGGIVTGLLLA
                     FGRSVGEVGISLMLGGNIVGRTETLSLAIYNAVFEGEFLRAAIFSIILTVIAIAVLLV
                     LNTVNRKRIPGLGM"
     gene            complement(1838..2584)
                     /gene="modA"
                     /locus_tag="PKN85_07235"
     CDS             complement(1838..2584)
                     /gene="modA"
                     /locus_tag="PKN85_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT44240.1"
                     /translation="MKGKPIIVGTALCLLLLFLGVSLASAQTLMIASAAGYKKPVSEA
                     IRSFEKATGIKTDAAFGNIQMVSSQAKQTGEISCIIGDRKFLTALGSTVAFVSYEPIG
                     KGVLVLAYRKGVSLDKVEDMASDKVKTVFMPQDKKAIYGIAGTEALKSYGYTSKLAGK
                     ITQVATVPQVVSYLIAGEADAGFINLTEAMANKERLGGYLVVPEDRYTEILIVAGVVK
                     GFENKPETKKFTDYLKTREAKGIFGKYGVK"
     gene            complement(2590..>2954)
                     /locus_tag="PKN85_07240"
     CDS             complement(2590..>2954)
                     /locus_tag="PKN85_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44241.1"
                     /translation="IEILPLVFTCFVSHDLPEIFKLCNRVIAFRDGRVVDDGNPYDIF
                     GRSKVSGKLQFVAEVLKVEKEDIVDVLTLLIGNSPVKVAVCNNRQETFMPGDKALVVS
                     KAFNPIIQKLNSNGKGGN"
BASE COUNT          561 a          921 c          878 g          594 t
ORIGIN      
        1 ggggtccgcc cctcacgctc cgatagaacc cttcacacac atggcattga agggggaacc
       61 cttccctctt atcgattggg actttccctg tttcacggac tctcagcgtt ttgcgcggat
      121 ccatgacagc gaccgccctc aagcctcgac aggagcgagg cggagacctg ccggtccgtg
      181 cagggcctgc gcgggatgga cggcatgccg cggaagattt gccgccgcag ccgcggaagg
      241 ggggtgttcc tcattcttcc gggagatggc acagatcgcg ctgacccgcg cgaggcaggc
      301 cgaggcccgc gcctgcctcg cgcctgccgc agcaccggcc ccaccttcgg agacggaaaa
      361 ccgcgaggaa ggagaggaag ggcccaccca acctttgcag ggaaccggag gtcccacctg
      421 gatagacctc cgcggcggtc ccgcccaggc ccttatcgtg tcgggggtgc tcaagcaggt
      481 ggtggaactg caccccggaa ggcggtatgc tctcgtcgca cggaccgggc tcacacggat
      541 cctcgagaaa cacccggcga tagagatgag cgggcatccg ccgaagggcg cccgggtcct
      601 tacgatccgg gccgggagct ttccgggtcc acaggaaggg gaatatcaaa ggctcgccgc
      661 ctctttcggc ctggcgacac ccgttgagga gcgtctctgg gtcccctggg ggttcgagga
      721 tgagcggaac ctcatggccc ttatcccccg gaaggagaag aatatcctca tctgcacctc
      781 ccgcgccccg cacaacgtga tccccatcga gcgctgggag gctctggcgg cgatgctcgg
      841 cagggaggga atgggagtcg tgcagacggg aggaaggaaa gacccctctg tccgcggcgc
      901 ctacaacatc ctcggccttc tcaccgaaag gcagttcata tccctgccgc gccattttga
      961 cgcggtggtc acctctgatg ccctcatgac gtgtgccgcc cgcctctgcc ggacccctgt
     1021 cgtaatcctc ggagactcgc ttggccccga cagcacgggc ctctcgctcg cggccgtccg
     1081 ccgcgccgtg gcccgggcgg tcgaagggca acaagggtag agtttctccc gccggcatta
     1141 catgcccagg cccggtatcc tctttctgtt caccgtgttg aggacaagga gcacggcgat
     1201 agctattacg gtgagtatga tggaaaatat cgccgccctg aggaactctc cttcgaagac
     1261 ggcgttgtag atggcaagcg acagtgtctc cgtgcgcccg acgatgttgc cgccgagcat
     1321 gaggcttatg ccgacctccc ccacggagcg tccgaaggcg agcaggaggc ccgtcacaat
     1381 gccgcccttg atgttgggaa ggaccacctt ggtgagcgtc tgggccctcg tcttcccaag
     1441 ggtggcggcc gcctcgatca gcgtgccgtc catctgctgc cgcgccgcct gcacgctttt
     1501 caccatgaaa ggcaggccca ccacgaagac ggcgacgagc acccccgagg gtgtgaatat
     1561 tatcttgaac gcgaacacct ccatcagaag atgcccgaga ggtccgttct tgccgaggac
     1621 aaggagcagg aagaacccca gcgccatcgg cgggaagatc atcggcaggg tggttatgat
     1681 ctcgacgacg gtcttcgccg gacttttctt ccccgcgagg tacaacccaa ggggcagacc
     1741 ggccgcgagc tgcaggatga gcgccaccag ggtcaccttg agcgtgagcg cgatggccgg
     1801 gtgcgtcaga tagtctgttg ccagcgtatg gggcatgtta tttcacgccg tatttcccga
     1861 atattccctt cgcttccctc gtcttgagat aatcggtgaa cttcttcgtt tccggcttgt
     1921 tctcaaaacc cttcaccacc ccggcgacaa taagtatctc ggtgtacctg tcttccggca
     1981 cgacgagata gccgcccagc cgttccttgt tcgccatggc ctcggtgagg ttgatgaacc
     2041 ccgcgtcggc ttctccggcg ataaggtagg agacaacctg gggcacggtg gccacctgtg
     2101 ttatcttgcc ggcaagcttt gatgtataac cataggactt cagcgcttcc gtccccgcaa
     2161 tgccgtagat ggcctttttg tcctgcggca tgaataccgt cttcaccttg tcggatgcca
     2221 tgtcctcaac cttgtcaagg ctcactccct tccgataggc gaggacaagg acacccttgc
     2281 ctatcggttc ataggagacg aatgccaccg tggaaccgag ggcggtcagg aacttcctgt
     2341 cgccgatgat gcagcttatc tcaccggtct gctttgcctg actggagacc atctggatgt
     2401 tcccgaaagc ggcgtcggtc tttatccccg tggccttttc gaaggatctt atcgcctccg
     2461 aaacaggctt cttatatccc gctgccgacg ctatcatcag ggtctgggcc gacgccaggg
     2521 acacacccag gaagagaagg agaagacaca gtgccgttcc gacaataatg ggttttcctt
     2581 tcatatgcct caattgcctc ctttgccgtt ggaattcagt ttttgaatga tggggttgaa
     2641 ggccttggag accacgaggg ccttgtcacc gggcatgaac gtctcctgcc ggttgttgca
     2701 gacggcgacc ttcacgggcg agttcccgat aaggagggtc agtacgtcca cgatgtcctc
     2761 cttctcgacc ttgagcactt ccgcgacgaa ctgcagtttg ccgctgacct tgcttcgacc
     2821 gaagatatcg tagggattgc cgtcatcgac gacacgcccg tccctgaatg ctatcacccg
     2881 gttgcacaat ttgaatatct ccggcaggtc gtggctgaca aagcaggtga agaccagtgg
     2941 aaggatctcg attt
//
LOCUS       DAOQTR010000275         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39253_length_2954_cov_3.377716, whole genome shotgun sequence.
ACCESSION   DAOQTR010000275 DAOQTR010000000
VERSION     DAOQTR010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39253_length_2954_cov_3.377716"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKN85_07245"
     CDS             complement(<1..347)
                     /locus_tag="PKN85_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNT44242.1"
                     /translation="MRVDFYDTTLRDGAQSEDIAFSLTDKLRITEKLDEFGIHYIEGG
                     WPGSNPKDLQYFKEVKKLSLRNARIVAFSSTVKANARPDTDPVMTALLDAGTDAVAIV
                     GKSWDLHVRDALRV"
     gene            complement(344..1576)
                     /locus_tag="PKN85_07250"
     CDS             complement(344..1576)
                     /locus_tag="PKN85_07250"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNT44243.1"
                     /translation="MLVVQKYGGTSVRDVQRIQNVARRAIEYKGRGMDVIVVVSAMSG
                     ETDRLLNLAHEIAKFPDEREVDVLISTGEQVTSALLAITLREMGHKAVSLLADQIRII
                     SDSSYGTARIASIEDAKLREEIKGGNIIVVPGFQGVDDGGNITTLGRGGSDTTAVALA
                     AALKADLCEIYTDVDGVYTTDPNICEKARRLDKISYDEMLEMASLGAKVLQIRSVELA
                     QKYNVPILVKSSLVEGKGTLVCKEDMIMMEKVVISGITYNKNETKITINKVPDKPGIA
                     SKIFSSLSAANIVVDMIVQNVSHENYNDITFTVVKADGKKAFKITEEIGAEIGAKVAM
                     DEDMAKVSIVGLGMRSHSGVASRMFTLLADEGINIELISTSEIKISCVIESKYGELAV
                     RALHKGFDLDAEDVTEES"
     gene            complement(1653..2081)
                     /gene="tsaE"
                     /locus_tag="PKN85_07255"
     CDS             complement(1653..2081)
                     /gene="tsaE"
                     /locus_tag="PKN85_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNT44244.1"
                     /translation="MGRTEYISKDPSETIDIGEMIGRSASAGEVYAIYGDLGAGKTQF
                     VKGIARGLGVPDWEYVVSPSFTLMNVYEGAMTLCHVDLYRLEEADLGDLNIEEFLAGG
                     IVAVEWAERSRWWEGVIEVRIEISGEMERKIVIIKHENQG"
     gene            complement(2044..>2954)
                     /locus_tag="PKN85_07260"
     CDS             complement(2044..>2954)
                     /locus_tag="PKN85_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNT44245.1"
                     /translation="DGEITDGDMMRGFLKRRLPSSHKGSFGHAVVIAGSPGKTGAAHM
                     ASLAALKIGAGLVTLVIPETLNAILEAKTTEVMTYPVADDGRGFFIPGSFDEIATFVE
                     DKDVVIMGPGLSQNEGVMELVRRIYREIDKPFVIDADGINAFRGHTRLLKKKGGEAVL
                     TPHPGELARLIDKTPKEVNDDRMGTALACARAWGGSILLNGAASVLATPDGQMFINPT
                     GNPSLAKGGSGDILTGFIGGLASQGYSLTRSTLLGAYLHGYMADTWVENHSDMDLLAL
                     DLVTGLGDAIEEIRNGTDRVYIERSL"
BASE COUNT          512 a          913 c          835 g          694 t
ORIGIN      
        1 accctgagag cgtccctcac gtgcaggtcc caggatttcc ccactatggc caccgcgtcg
       61 gtccccgcgt cgagaagggc cgtcatgacc gggtccgtgt cggggcgggc gttcgccttc
      121 accgtgctcg aaaaggccac gatgcgggcg ttcctgaggg agagcttctt tacctccttg
      181 aagtactgca ggtctttcgg gttcgagccc ggccagcctc cctctatgta atggatgccg
      241 aactcgtcga gcttttccgt tatcctcagt ttatcggtga ggctgaaggc gatatcttcc
      301 gactgggccc cgtccctgag ggtggtatcg taaaagtcga ccctcaactc tcctccgtta
      361 catcctcggc atcaaggtcg aaccccttgt gaagcgcccg taccgcaagt tcaccgtact
      421 tgctctcgat aacgcaggat atcttgatct ccgacgtgga tatgagctct atgttgatcc
      481 cctcgtcggc aaggagcgtg aacatcctgg aggcgacacc ggaatgggag cgcatgccga
      541 gcccgacgat ggagaccttg gccatatcct cgtccattgc gacctttgcc cctatctcgg
      601 cccctatctc ctcggtgatc ttgaaggcct ttttcccgtc ggccttcacg accgtgaagg
      661 tgatatcgtt gtagttctcg tggctgacat tctggacgat catgtcgacg acgatgttgg
      721 ccgcgctcag ggagctgaat atcttcgatg cgatacccgg cttgtcgggc accttgttga
      781 tggttatctt cgtttcgttc ttattgtagg tgataccgga aatgacgacc ttttccatca
      841 tgatcatgtc ctccttgcag acaagtgttc cttttccctc gacgagcgat gatttgacga
      901 ggatgggcac gttgtacttc tgtgcgagtt cgacggaccg tatctggagt accttcgccc
      961 ccagggatgc catctcgagc atctcgtcgt aggatatctt gtcaaggcgt ctcgcctttt
     1021 cgcagatgtt gggatcagta gtgtagacgc cgtccacgtc ggtgtagatc tcgcacaggt
     1081 cggccttgag ggccgccgca agggcgacgg cggtggtgtc cgagccgccc cttcccagtg
     1141 tggtgatgtt tcctccgtca tcgacgccct ggaacccggg cacgacgatg atgttgcctc
     1201 ccttgatctc ctcccggagc ttcgcgtcct cgatgctggc gatgcgggcg gtcccgtagc
     1261 tcgagtcgct gatgatcctt atctggtccg ccagcaatga cacggccttg tggcccatct
     1321 ccctgagggt gatcgcgaga agggcggacg tgacctgctc tcccgtggag atgagcacat
     1381 ccacttccct ttcatcgggg aatttcgcta tctcgtgggc caggttgaga agcctgtccg
     1441 tctcgccgga catggctgag acgaccacga tgacgtccat cccgcgcccc ttgtactcga
     1501 tggcacgcct ggccacattt tgtatcctct gcacgtccct taccgacgtg ccgccatatt
     1561 tctggacaac gagcattgta tcctccaggt atagggtgta agggtatggt accgggaatc
     1621 aagagaggtt cccgccgcgg ttaccgcggt actcaaccct gattttcatg cttgataata
     1681 acaattttcc gttccatttc tccactaatt tctatgcgca cctctatcac tccctcccac
     1741 cagcgggacc tttccgccca ctcgacggcg acgatccctc cggcaaggaa ctcttcgata
     1801 ttgaggtcgc caaggtccgc ttcttcaagg cgatagaggt ccacatgaca gagcgtcatc
     1861 gcgccttcgt acacgttcat gagcgtaaac gaggggctca ccacgtattc ccagtcgggg
     1921 acgccaaggc cccgcgcgat gcccttgacg aactgggtct ttcccgctcc gaggtcgccg
     1981 tagatcgcgt agacctcgcc ggccgaggcc gagcggccta tcatttcccc gatgtctatc
     2041 gtttcagagg gatctttcga tatatactct gtccgtccca ttacggatct cctcgatagc
     2101 gtcgcccaat cccgtcacga ggtcaagggc gagaaggtcc atgtcggaat ggttctccac
     2161 ccaggtgtcg gccatgtacc cgtggaggta cgccccaaga agggtgctcc gggtcaggct
     2221 gtagccctgg gaggcaagac cgccgataaa gcccgtcagg atatccccgc ttcctccttt
     2281 cgcgagggaa ggatttcccg tggggttgat gaacatttgg ccatcgggcg tggcgagcac
     2341 gctggcggct ccgttgagga ggatgctccc gccccatgcg cgggcacagg cgagcgccgt
     2401 gcccatccga tcgtcgttca cctctttcgg ggtcttgtcg atgaggcggg ccaactcccc
     2461 cggatgcggc gtcaggacgg cctcgcctcc tttcttcttc aggagccgcg tgtgtccccg
     2521 gaacgcgttg atgccgtcgg cgtctatgac gaagggtttg tcgatctccc ggtatatcct
     2581 tcgtacgagc tccatgaccc cctcgttctg ggaaaggccg ggacccatga tcaccacgtc
     2641 cttatcttcc acgaaggtgg ctatctcgtc gaaggacccg gggatgaaga aacccctgcc
     2701 gtcatccgcg acgggatatg tcatgacctc cgtcgtcttt gcctccagta tggcgttcaa
     2761 tgtctcgggg atgaccagtg tgacaagccc ggcgccgatc ttgagggcgg cgagcgatgc
     2821 catgtgcgcc gcccctgttt tccccggcga accggcaatg acgacggcgt ggccgaagct
     2881 ccccttgtgg gacgagggga ggcgcctttt gagaaagccc cgcatcatgt cgccgtcggt
     2941 tatctccccg tcga
//
LOCUS       DAOQTR010000276         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39254_length_2954_cov_3.325974, whole genome shotgun sequence.
ACCESSION   DAOQTR010000276 DAOQTR010000000
VERSION     DAOQTR010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39254_length_2954_cov_3.325974"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>323)
                     /locus_tag="PKN85_07265"
     CDS             complement(<1..>323)
                     /locus_tag="PKN85_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44246.1"
                     /translation="TKMGIAADGVYVDLGIGDYSRISLEKIKAVADRIGRKVHIFHVP
                     DVFGRNISEVAKGLRRAPCSACGMIKRYVMNKVCMDKDYNLLLTGHNLDDEAAALFGN
                     ILYWK"
     assembly_gap    324..333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<334..520)
                     /locus_tag="PKN85_07270"
     CDS             complement(<334..520)
                     /locus_tag="PKN85_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginosuccinate synthase"
                     /protein_id="HNT44247.1"
                     /translation="MKCRVCGQTASISLRSHKTAFCDTHFITFFEKRVATTIRRYRLI
                     GETDRPVVAVSGGKDSLS"
     gene            complement(517..705)
                     /locus_tag="PKN85_07275"
     CDS             complement(517..705)
                     /locus_tag="PKN85_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNT44248.1"
                     /translation="MHVNWEGKEYVFEKPMLVSRLLQELNVSQEGHLVVANNRLVTED
                     HRLGADDNVKVIRVVSGG"
     gene            944..>2954
                     /locus_tag="PKN85_07280"
     CDS             944..>2954
                     /locus_tag="PKN85_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNT44249.1"
                     /translation="MNWEKLTIKAQEAIQEAQKKAESLGQQSIENEHLLFALISQKDG
                     IIRPILEKLGVKADMIARDLDRELAKIPRVEGVSQTYISSRFKQTLDTAFTEAERLKD
                     EFVSTEHLLIGIADGKDGPAAATLRIYGITKDSIYAVLKDLRGTQRVTDQAPEEKYQA
                     LQRYCRDLTEEARKGKLDPVIGRDEEVRRVMQVLSRRTKNNPVIIGEPGVGKTAIVEG
                     LAQRIISGDIPETLKNKRVLSLDLGSLLAGAKYRGEFEDRLKAVLKEIDEAAGTIVLF
                     IDELHTIVGAGGAQGAVDASNMLKPALARGELRCIGATTLDEFRKYIEKDAALERRFQ
                     PVYVGEPSVEETIAILRGLKEKYELHHGVVIKDPALIAAATLSHRYITDRFLPDKAID
                     LVDEASSRLRMEIDSVPTEIDEIDRKIIQSQIEIEALKKEKDEASKERMKKLEDELAE
                     MKKEVQEKRGHWEKEKALITGISETKEKIDQARTQADDLERRGDFGRVAEIRYGTISS
                     LEQELKQRNDELAALQKGKKMLKEEVDEEDIARVVSKWTGIPVSKMLEGEMDKLVHME
                     DNIHKRVIGQDEAIGAVANTIRRARAGLQDPNRPLGSFLFLGPTGVGKTELARALAEF
                     LFDDEQAIVRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGYLTEAVR"
BASE COUNT          671 a          868 c          921 g          484 t
ORIGIN      
        1 tcttccagta aaggatgttc ccgaagaggg cggcagcctc gtcatcaagg ttgtgtccgg
       61 taagcagaag gttgtagtcc ttgtccatgc agaccttatt catgacgtag cgcttgatca
      121 tgccgcatgc cgaacagggt gcccggcgca gccccttcgc cacctcgctg atgttcctgc
      181 cgaaaacgtc ggggacatga aagatgtgga ctttcctgcc gatcctgtcg gccacggcct
      241 ttatcttttc cagcgagatg cgggagtagt cgcctatgcc gaggtcgacg tagacgccgt
      301 cggcggcgat gcccatcttc gtcnnnnnnn nnngggacag ggagtccttg cctcccgaca
      361 cggccacgac ggggcggtcg gtctcaccga tgagccggta acgccggatg gtcgtggcca
      421 ccctcttctc aaaaaaagta atgaaatgtg tgtcacagaa ggcggtcttg tgggaccgca
      481 gactgatgct tgccgtctgt ccgcataccc tgcatttcat ccgcccgata cgacccgtat
      541 gacctttacg ttatcgtcgg caccgagcct gtggtcttcg gtgacgagcc ggttgttcgc
      601 gacaacgaga tggccctcct ggctgacatt gagttcctgc aggagccggg acacgagcat
      661 cggcttctca aagacgtact ctttcccttc ccagttgacg tgcatggcta attctttagc
      721 acaacgatgg ggtgatgtca aaaaggctcc ggggacggta tcggcgcggg ccggtgtgat
      781 gaacgcacgg gaaggcagcc ggccgcccat tgattccggg gatatcgtgc ttatatttca
      841 gacaagattt cggatgtcac cctatgaagc aggccgcaga aggttgtccc cggggcgcga
      901 tgggccacga agggtctgac atatactgtc acgaggtgaa catatgaact gggagaaact
      961 gaccataaag gcccaggagg ccatccagga ggcgcagaag aaggccgagt ctctgggcca
     1021 gcagagcatc gagaacgagc acctgctctt tgccctcata tcgcagaaag atgggatcat
     1081 acggcccata ctggaaaagc tcggcgtcaa ggcggacatg atagcccgcg acctcgaccg
     1141 cgaacttgcc aagataccgc gcgtggaagg cgtgagccag acctatatat cgtcgcgctt
     1201 caagcagacc ctcgacacgg cgttcacgga agcggaaagg ctcaaggacg agttcgtgag
     1261 taccgaacac ctcctcatcg gcatagccga cgggaaggat ggaccggcgg cggccactct
     1321 caggatatac ggcataacaa aggacagcat ctacgcggtc ctcaaagact tgaggggcac
     1381 ccagcgggtg accgaccagg ccccggagga aaaataccag gcgctccagc gctactgccg
     1441 ggacctgacg gaagaggccc gcaagggcaa gctcgacccc gtcatcggcc gcgacgagga
     1501 ggtccgcagg gttatgcagg tcctgtcgcg caggacaaag aacaaccctg tcatcatcgg
     1561 cgagcccggc gtgggcaaga cggccatcgt ggaaggtctc gcccagagga tcatatccgg
     1621 agatatcccg gagaccttga agaacaagcg cgtcctctcc ctcgacctgg ggtccctgct
     1681 cgcgggagcg aagtaccgtg gtgagttcga ggacaggctc aaggccgtcc tcaaagagat
     1741 cgacgaggcg gcggggacga tcgtcctgtt catcgacgag ctccacacca tcgtcggcgc
     1801 cggcggtgcc cagggcgcgg tcgacgcgtc gaacatgctc aagcccgccc tggcccgggg
     1861 cgagctgcgc tgcataggcg ccacgaccct cgacgagttc cgcaagtaca tagagaagga
     1921 cgccgcgctg gagcgccgtt tccagccggt ctacgtgggc gagccctccg tcgaggaaac
     1981 gatcgccatc ctgaggggcc tcaaggagaa gtacgagctc catcacgggg tcgtcataaa
     2041 agaccccgcc ctcatcgcgg cggcgacgct ttctcatcgc tacatcaccg accggttcct
     2101 ccccgacaag gccatcgacc tcgtggacga ggcgtcatcg cggctgcgca tggagataga
     2161 cagcgtcccc accgagatcg acgagatcga tcggaagatc atccagtccc agatcgagat
     2221 cgaggcgctg aagaaggaaa aggacgaggc atcgaaggag cgcatgaaga agctcgagga
     2281 cgaactcgcc gagatgaaga aagaggttca ggagaagcgc gggcactggg agaaggagaa
     2341 ggcccttatc acgggcatct ccgaaacaaa agagaagatc gaccaggcga ggacacaggc
     2401 cgacgatctg gagcgcaggg gagattttgg acgggtggcg gagatccgat acggcaccat
     2461 atcctccctg gagcaggaac tgaagcagag gaacgacgag ctcgccgccc tccagaaggg
     2521 gaagaagatg ctcaaggagg aggtcgacga agaggacata gcccgcgtcg tctccaagtg
     2581 gacgggcatc cccgtctcca agatgctcga aggggagatg gacaagctcg tgcacatgga
     2641 agacaacatc cacaagcgcg tcatcggaca ggacgaggcc atcggcgccg tcgcaaatac
     2701 catccggcgc gcccgtgcag ggctccagga ccccaaccgg cccctggggt cattcctctt
     2761 cctcggcccg acgggcgtcg gcaagacgga actggcccgg gcccttgcgg aattcctctt
     2821 cgatgacgag caggccatag tccgcatcga catgagcgaa ttcatggaga agcactccgt
     2881 gtcccgtctc atcggggcac ccccgggata cgtgggatac gaggaaggcg gttacctcac
     2941 cgaggcggtc cgca
//
LOCUS       DAOQTR010000277         2952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39279_length_2952_cov_3.916810, whole genome shotgun sequence.
ACCESSION   DAOQTR010000277 DAOQTR010000000
VERSION     DAOQTR010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39279_length_2952_cov_3.916810"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..1223
                     /locus_tag="PKN85_07285"
     CDS             285..1223
                     /locus_tag="PKN85_07285"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNT44250.1"
                     /translation="MPYDLCRTIIDNMSRVIVGKERSIELLMVALLADGHVLLEDIPG
                     VGKTLIAKSLAKSIGATFKRVQFTPDLLPSDITGFNVYDQQKGEFIFQFGPVMTHVLL
                     ADEINRTIPRTQSSLLESMEERQVTVDGKTYPLPHPFFVMATQNPIDLEGTFPLPEAQ
                     LDRFLLRVRLGYPGQDEEVAILKRFQENDPLRVLDAVATPREVSRLQEVRKGIRVSEP
                     VRKYITDVVRATRDNPACHLGASPRGSLGLMRAGQAMAILRQREYVLPDDIKELALPV
                     LAHRLILHEEVRLQGRSQEQVVGEILETVHVPTETD"
     gene            1223..2431
                     /locus_tag="PKN85_07290"
     CDS             1223..2431
                     /locus_tag="PKN85_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT44251.1"
                     /translation="MEAISRRPSSLFITLPALALGMVLLITALVYKQRDLSILTLLLL
                     CAATGAKLWTAFGRRGTQCATDADTKRVFPGNPIELTLTLTSTTFLPLWVRADIPLNG
                     LCLTSRNSETRLTGESGLWRDTGRFRWDVSAPRRGLFRPGPVGLTCGDLFGFFPKESE
                     VDDPLEIIVYPRLVPLKAFSIIRRDFFGIPGDMSPVRDPVYILGTHDYQHGRPARFIH
                     WKASARRHVLQEKVFEPTEQEKVLFVVAVEDFALSGAADAFERSLEAVASMAALFDRQ
                     GVATGLVTDSAVEGKHTSFRSVRRDAGHLGSLLEILARVRMEKGRGLAEGLRAMSALP
                     FGVTCLCFSHRMDDSTRAVEEYLRRRRVPVIHYTWVPPSVADRGIRPSERGSVRSTRD
                     LFAHKGKGEQ"
     gene            2428..>2952
                     /locus_tag="PKN85_07295"
     CDS             2428..>2952
                     /locus_tag="PKN85_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44252.1"
                     /translation="MKRTEKALLALCGAILDFTWLYAWAAFSMVSVGTAPAAPSDTAA
                     IFTAAAMLSLLCAGRGLRILTVGLLQGAGIVLAALWTVRTMSDAMTPLFDIRWITGFF
                     GAPRSAMEWLGLGIALFFSTALWLGGAFFAVRPRTHEKICSRFDLGLAAFFTLVLIKL
                     AMAVKGNILTGDTMT"
BASE COUNT          551 a          974 c          857 g          570 t
ORIGIN      
        1 tgtccagcat atccacgctc cccgattcta caggagcaat attcgtgcca ggatcttcaa
       61 gcatggaccc gtcccttgga agcccccact accatctgtg ccgtatgcca acggatcccg
      121 gctgcccgtc ccggtgaccg gagagggcag cgtcacaggc aaggcaaaag atcttccgca
      181 ggattgtgaa ggggcaatcc ttcgacaatg gtccccccct gtgttatact cttgacggtt
      241 ttgaggtatt cgatattaca acccctaccc cggagaaccc tgttatgcct tatgatctgt
      301 gcagaaccat catcgacaac atgtcccgcg tcatcgtcgg caaggaaagg tccatcgaac
      361 tgctcatggt ggcgcttctg gccgacggtc atgtgcttct ggaggacatc cccggcgtcg
      421 gcaagacgct catcgcgaaa tccctggcga aaagtatagg ggcgaccttt aaaagggtcc
      481 agttcacccc ggacctcctg ccgtcggaca tcaccggctt caatgtctac gaccagcaga
      541 agggtgagtt catcttccag ttcgggccgg tcatgaccca tgtgctcctg gcggacgaga
      601 taaaccgcac catcccccgg acacagtcga gtcttctcga gagcatggaa gagaggcagg
      661 tgaccgtcga cgggaaaacg taccccctcc cccacccctt ctttgtcatg gccacacaga
      721 accccatcga cctcgaaggg acattcccgc tgccggaggc ccagctcgac cgttttctcc
      781 ttcgagtccg tctcggctat ccgggacagg acgaagaggt tgccatcctg aagcgcttcc
      841 aggagaacga ccccctgcgg gtgctcgacg cggttgccac gccccgggag gtctcccggc
      901 tgcaggaggt ccgaaaaggc attcgcgttt cggaaccggt caggaagtac ataaccgatg
      961 tggtccgcgc cacgcgcgac aaccccgcgt gtcacctggg agccagcccc cggggatcgc
     1021 tcggcctcat gcgcgcgggg caggcgatgg ccatactgag gcagcgggaa tacgtccttc
     1081 ccgacgatat caaggaactg gccctccccg tcctcgccca tcgccttata ctccacgagg
     1141 aggtccggct ccagggaagg tcacaggaac aggtcgtcgg cgaaatactc gagaccgtgc
     1201 acgtccccac tgaaacggat tgatggaagc gatcagtcga cggccctcaa gcctcttcat
     1261 aacccttccc gcgctcgcac tgggaatggt gcttctcatc acagcgcttg tctacaagca
     1321 aagggacctt tcgatattaa cacttcttct gctttgcgcc gcgacaggcg cgaaactgtg
     1381 gacggccttc gggcgccgcg gcacacaatg cgcgaccgat gcggacacca agagggtctt
     1441 tccgggaaac ccgatcgaac tcacgctcac gctcacgagc accacattcc ttcccctctg
     1501 ggtccgagcc gacatccccc ttaacggcct ttgtctcacc tccaggaata gcgaaacacg
     1561 tctgacgggc gagtccggac tgtggcgcga caccgggcgc ttccgctggg atgtgtctgc
     1621 cccgcgccgc gggctcttcc gtccgggacc cgtcggtctc acctgcggcg acctcttcgg
     1681 gttcttcccg aaggaaagcg aggtggatga ccccctggag atcatagtct acccgcggct
     1741 cgttcccctc aaggcctttt ctatcatccg cagggatttt ttcggcatcc ccggggatat
     1801 gagccccgtg cgcgatcccg tctacatact cggcacccat gactaccagc acgggcggcc
     1861 ggcgaggttc atacactgga aggcgagcgc gcgcaggcac gtcctgcagg aaaaggtctt
     1921 tgagcccacg gagcaggaaa aggtcctttt tgtcgtggcg gtggaggatt tcgcgctctc
     1981 cggcgctgcc gatgccttcg agagaagcct cgaggcggtg gcctccatgg cggcgctctt
     2041 cgacaggcaa ggtgtggcga cagggctcgt gacggactcg gcggtcgagg gaaagcacac
     2101 ctctttcagg tccgtccgcc gggacgccgg ccatcttgga tcgcttctcg agatcctggc
     2161 gcgcgtccgc atggagaaag gacgcggcct tgcggagggg ctgcgggcga tgagcgccct
     2221 tcccttcggt gtcacctgcc tgtgtttctc ccaccggatg gatgattcaa cccgcgcggt
     2281 ggaggaatac ctcagaagac gccgggtacc cgtcatccac tacacctggg tcccgccttc
     2341 cgtggcagac aggggcattc gcccgtcaga gcgcggctcc gtccgctcca cccgggacct
     2401 cttcgcgcac aaagggaaag gcgagcaatg aaaagaaccg aaaaagcgct tctcgccctg
     2461 tgcggcgcaa tcctcgactt cacatggctg tatgcgtggg ccgcgttctc catggtgtcg
     2521 gtgggaactg cccccgctgc gccctctgac accgcggcca tcttcaccgc cgccgccatg
     2581 ctcagcctcc tctgtgcggg aagaggattg aggatcctca ccgtcgggct tcttcagggc
     2641 gcagggatcg tcctcgctgc cctgtggacc gtccgcacca tgagcgatgc gatgacccct
     2701 cttttcgaca tccggtggat cacgggattc ttcggggcac cgcgcagcgc tatggagtgg
     2761 ctggggctgg gcatcgctct cttcttcagc acggccctgt ggctcggcgg cgccttcttc
     2821 gccgtgagac cccggaccca tgagaagatc tgctcccgct tcgacctcgg cctcgcggcc
     2881 ttttttaccc tcgtcctcat caagttggcc atggcggtga agggaaacat actcaccggg
     2941 gatacgatga cg
//
LOCUS       DAOQTR010000278         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39582_length_2937_cov_3.423317, whole genome shotgun sequence.
ACCESSION   DAOQTR010000278 DAOQTR010000000
VERSION     DAOQTR010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39582_length_2937_cov_3.423317"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1964
                     /gene="dxs"
                     /locus_tag="PKN85_07300"
     CDS             111..1964
                     /gene="dxs"
                     /locus_tag="PKN85_07300"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNT44253.1"
                     /translation="MLLQTINSPEDLKKLSIAQLVELAGEIRPFITDVISRTGGHLAS
                     NLGAVELTIALYYVFNPPGDKIVWDVGHQCYTCKLLTGRRESFCTIRQDGGLSGFPCK
                     AESVYDVFDMGHASNSISVAAGLAESKRKLGSTSKIIAVIGDGSLTGGMAFEALNHAG
                     HLRSDIIVILNDNEMSISKNVGGLASHLNRIMTGEFMSSIREELKNRMKTMPTIYKTA
                     RYLEEAVKGIFGPGMIFEELGFTYVGPVDGHNMEHLLETFRNVSRFKGPLLVHVITRK
                     GKGYTHAEDDPERFHGISTFELTTGNTLSKAKKTYTDIFGDTIIDLAKMDERVVAITA
                     AMGLGTGLDKFSDMFPDRFYDIGIAEQHGVTFSAALALGGLRPFVAIYSTFLQRAYDQ
                     IILDVCLQNLPVVFAVDRSGIVGQDGPTHHGVYDLSYFRHIPNMVLMAPKDENELRQM
                     LYSGYRYGRPVAIRYPRGEALGVPIEDDFSEIPLGTWESLREGKDITMIACGNMVAAA
                     VEAADVLAAEGVDAGVVNGRFIKPMDEKLLAEVAERTGRILTLEENVVIGGFGSGVGE
                     ALARAGSHIPLRSMGIPDTFVTHGSQGMLRKSLGIDLDGIVRTVRTWLGKE"
     gene            1946..2695
                     /locus_tag="PKN85_07305"
     CDS             1946..2695
                     /locus_tag="PKN85_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNT44254.1"
                     /translation="MARERIDILLARRSLAPSREKAQVLIMAGEVYVDGQRVLKADRK
                     VDETVCIEVRGNPIPYVSFGGVKLKAALDAFGLTVKGLTALDIGSSTGGFTDCLLAEG
                     ASKVYAVDSGTHQLHEKLRGDERIVLRENFNARYLTLTDIGERVDVVTIDVSFISLKK
                     IIPAAVGVLSDAGRIVSLVKPQFEVGRYNVGKGGIVKDGEKIESVLMDVRQFGETLGL
                     DAVGTVEAPREREKKNREYFILWVRSGDPDR"
     gene            2665..>2937
                     /locus_tag="PKN85_07310"
     CDS             2665..>2937
                     /locus_tag="PKN85_07310"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNT44255.1"
                     /translation="MGKIGRPRQVRLFASIIFRRDADVEGALRVLGGAMGPIEEKTPP
                     ALFTHTTYYDREMGEGLMRSFILFSPLVDREVLPDIKIFTNATEDLF"
BASE COUNT          646 a          871 c          880 g          540 t
ORIGIN      
        1 tacaaactgg agcaactcgt cgatgaagca gtgagatcga tcgcattcct gggagagaac
       61 ggccgcatac tcgaggacat agcgcgcttc atcggcaaca gggtttcgta atgcttctcc
      121 agacaatcaa ttctcccgag gaccttaaaa aactgtctat tgcccagctc gtcgagcttg
      181 ccggcgagat acgccccttc atcacggacg tcatatcccg gactggcgga catctcgcct
      241 cgaacctcgg cgccgttgag ctcaccatcg ctctctatta cgtcttcaac cctcccgggg
      301 acaagatcgt gtgggatgtc ggccatcagt gctacacgtg caagctcctg acgggccggc
      361 gggagagctt ttgcaccatc cgccaggacg ggggactctc cggcttcccc tgcaaggccg
      421 agagtgtata cgatgtgttc gacatgggcc atgccagcaa ctccatatcc gtcgccgcgg
      481 ggcttgccga gagcaagaga aagctgggat cgacctccaa gatcatagcc gtcatcgggg
      541 atggttcgct gacgggcggc atggccttcg aggccttgaa ccatgccggg cacctcagaa
      601 gcgatatcat cgtcattctc aacgataacg agatgtcaat atcgaagaac gtcggagggt
      661 tggcgagcca cctcaaccgg atcatgacgg gagaattcat gagcagcatc cgggaagaac
      721 tgaagaaccg gatgaaaacc atgcccacca tctacaagac ggcgcgatac ctggaggagg
      781 ccgtcaaggg catcttcggg ccgggcatga tcttcgagga actcggtttc acctacgtcg
      841 gtcccgtcga cggccacaac atggaacacc tcctggagac cttcaggaac gtgagcaggt
      901 ttaaaggacc gctactcgtc cacgtcatca ccaggaaggg gaagggctac acccacgccg
      961 aggatgatcc cgagcggttc cacggcatct caacctttga gctcaccacg ggaaacaccc
     1021 tctccaaggc gaagaagacc tataccgaca tcttcggcga cacgataatc gacctggcaa
     1081 agatggatga gagggtggtg gcgatcacgg ccgcgatggg cctcggcacg ggcctcgaca
     1141 agttctccga catgttcccc gacaggttct acgacatagg catcgccgag cagcacgggg
     1201 tgaccttttc cgcggccctc gccctcggcg ggctcagacc ctttgtcgcc atctattcca
     1261 cctttctgca gagggcctac gatcagatca tcctcgacgt gtgccttcag aacctgcccg
     1321 tggtgttcgc ggtggataga agcgggatcg tgggccagga cgggcccacc catcacggcg
     1381 tttacgacct cagctatttc cgccacatcc cgaacatggt cctcatggcg ccgaaggacg
     1441 agaacgagct tcggcagatg ctctattccg ggtaccgcta cgggaggccc gtcgccatcc
     1501 gctatccgcg gggcgaagcc ttgggcgtgc ctatcgagga cgatttcagc gagatacctc
     1561 tcggcacctg ggagtcgctc cgtgagggca aggacatcac catgatagcc tgcgggaaca
     1621 tggtggccgc ggccgtggaa gccgccgatg tgcttgcagc ggaaggtgtg gacgccggcg
     1681 tcgtcaacgg caggttcata aaaccaatgg atgagaaact ccttgctgag gtagccgaaa
     1741 ggactgggcg catcctcacc ctggaagaga acgtggtcat cggcggattc ggcagcgggg
     1801 tcggggaggc cctcgccagg gcaggttcgc atatccctct cagatcgatg ggcattcccg
     1861 acacgttcgt gacccatggg agtcagggaa tgctacgcaa atctctcggc atcgacctcg
     1921 atgggatcgt caggacggtc cggacgtggc tagggaaaga atagacatcc tccttgccag
     1981 gcgctccctg gcaccatccc gggaaaaggc gcaggtgctc atcatggccg gagaggtcta
     2041 tgtcgacggc cagagggtct tgaaagcgga ccggaaggtc gacgagaccg tctgcatcga
     2101 ggtcagggga aaccccatcc cctatgtgag tttcggcggg gtcaagctca aggccgcgct
     2161 cgacgctttc ggcctcaccg tgaagggcct gacggccctc gatatcggtt catcgacagg
     2221 cgggttcacc gattgcctcc tcgcggaagg ggctagcaag gtgtatgccg tcgattccgg
     2281 gacgcaccaa ctccacgaga agctccgggg cgacgaacgg atagtcctga gggagaattt
     2341 caacgcccgt tacctgaccc tcaccgacat cggcgaaagg gtcgatgtcg tcacgataga
     2401 cgtgtcattc atctccctga agaagatcat ccccgccgcc gtgggcgtcc tcagcgacgc
     2461 tggccgaata gtctccctcg tgaagccgca gttcgaagtt ggaagataca atgtgggcaa
     2521 aggaggcatc gtgaaggacg gggagaagat agagtccgtc ctcatggacg tcaggcagtt
     2581 cggtgaaacc ctgggccttg acgcggtcgg gaccgtggag gctcccaggg aaagagaaaa
     2641 gaagaacagg gaatacttca ttctatgggt aagatcgggc gaccccgaca ggtaagactt
     2701 ttcgccagca tcatcttccg ccgcgacgcg gacgtcgaag gggccctgcg cgtcctcggc
     2761 ggcgcaatgg ggcctatcga ggagaagacc ccgccggccc tcttcacgca cacgacatac
     2821 tacgacaggg aaatgggcga agggctcatg cggtctttta tcctcttctc ccctctcgtc
     2881 gaccgggagg tccttcccga tatcaagata ttcacgaatg ccaccgagga tctcttc
//
LOCUS       DAOQTR010000279         2936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39605_length_2936_cov_3.582784, whole genome shotgun sequence.
ACCESSION   DAOQTR010000279 DAOQTR010000000
VERSION     DAOQTR010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39605_length_2936_cov_3.582784"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKN85_07315"
     CDS             complement(<1..279)
                     /locus_tag="PKN85_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNT44256.1"
                     /translation="MAAIFEDNSFSIGRTPLVRLNRVTAGAKAMVLAKVEGRNPAYSV
                     KCRIGASMVWDAEKTGRLRPGMEIVEPTSGNTGIALAFVAAARGYKLTL"
     gene            complement(439..1866)
                     /locus_tag="PKN85_07320"
     CDS             complement(439..1866)
                     /locus_tag="PKN85_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNT44257.1"
                     /translation="MNPIEMPPDVMLKKSPFLVVRQLRDDEVRVYSKLHGNLTSYGSE
                     IIDVLGAFDRPVSMGTAAAQVSAMCRCEPSVLMRELYEKRFLVEGDRDERDMFAEYVA
                     MARHRNEVARISKVTFLLSAKCNLSCKGCYHSFYDFKSGHMTSEFADSVLSGLFPYLK
                     KRGIRSLLISFLGYEPLLNFETMRQVCDRAAAMSEEYGIDTTFKIFTNAFTLNEDVYD
                     WIVKNGPRLSFKVSLDGVREDNDQRRVDRAGKGTFDRVVGNLKRIIATGAECDVLTTL
                     SKINFANIERFVDEMAAIGIRNITANIFCGQSPEERRIELSEEERFEAIKRMDLATEK
                     HGIEFDGEWKFAVVQMITGAHFTCPAGMRQMVFCADGAIHPCQRFAGTELTFGVFGDD
                     FWEKLGEGQCEGYERWIADLYDGVMDRVKEEHADLTGWSCPFVPFIRGECLGKHVEKD
                     FNERLIEYYVTRPMDRIIAKSRIHC"
     gene            2155..2643
                     /locus_tag="PKN85_07325"
     CDS             2155..2643
                     /locus_tag="PKN85_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNT44258.1"
                     /translation="MEALKDWLRPELIWFLIGLALMLLEFVLPGFIIFFFGVGAWVVA
                     LICLFADVSINAQLIVFLVASVLLLLSLRKWMRGVFVGRKKFGRPSDDDQLEFTGKRA
                     VVTRTIDPVMGGKVELHGTSWNAETDGDEPVEEGAAVEITGKQGLTLKVKKYPAKGGN
                     GQ"
     gene            2640..>2936
                     /locus_tag="PKN85_07330"
     CDS             2640..>2936
                     /locus_tag="PKN85_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNT44259.1"
                     /translation="MNFFTVIIVGLIILVVIAFLKTIRVVPQKVAFIVERLGKYNATL
                     DAGLHVLIPFIDKVSYRHTLKEQAIDVPSQTCITRDNIAVEVDGILYLQVIDPQK"
BASE COUNT          592 a          896 c          774 g          674 t
ORIGIN      
        1 cagggtcagt ttataccccc tggctgccgc gacgaaggcc agggcgatgc cggtattgcc
       61 cgatgtcggc tctacgatct ccatgcccgg tctcaacctg cccgtcttct ccgcgtccca
      121 taccatcgag gcgccgatcc tgcattttac cgagtaggct ggattgcgtc cctcgacctt
      181 cgcgagcacc atcgctttcg cgcctgccgt caccctgttg agcctgacga gcggcgttct
      241 gcctattgag aacgagttgt cctcgaatat cgctgccatg ggcggcctcc ttgtctatgc
      301 cttcagatcc gacccggatc gggaccgaag gatggtataa cttttattat atcattttgg
      361 cgccccgggc aagacatcga ccgcctccgt cacagccgga aacggacaag cgcagaaggg
      421 ggccccggga ccgaaacctc agcaatgtat acgcgatttc gcgattatac ggtccatggg
      481 gcgcgtgaca tagtactcga tgaggcgttc gttgaaatcc ttctcgacat gtttgccgag
      541 acattcgccc cgtatgaaag ggacaaaggg acaactccag ccggtaaggt ccgcgtgttc
      601 ttcctttact ctgtccatca cgccgtcata gaggtcggcg atccagcgct cgtaaccctc
      661 gcattggcct tcgccaagct tctcccagaa atcgtccccg aaaaccccga aggtgagctc
      721 cgttccggca aaacgctggc agggatggat cgcgccgtcg gcgcagaaga ccatctgcct
      781 cattcccgcc ggacaggtga agtgggcgcc ggttatcatc tgcacgacgg cgaacttcca
      841 ctccccgtcg aactcgatgc cgtgtttctc cgtggcaagg tccatccttt ttattgcctc
      901 gaacctctcc tcttccgaca gctctatcct tctctcctcg ggcgattgac cgcaaaatat
      961 attggcggtg atgttcctga tgccgatggc ggccatttca tccacgaagc gctcgatatt
     1021 ggcgaagttg atcttgctga gggtcgtcag gacgtcgcac tccgcgcccg tggcgattat
     1081 ccttttcagg ttgccgacga ccctgtcaaa cgttcctttt ccggcgcggt ccacccttcg
     1141 ctggtcattg tcctccctga ccccgtccag gctcaccttg aaactcagcc ggggtccgtt
     1201 cttcactatc cagtcataga catcttcatt cagggtgaag gcattcgtga atatcttgaa
     1261 cgtggtgtct attccgtatt cctcggacat ggccgccgcc cggtcacaga cctgcctcat
     1321 ggtctcgaaa ttcaggagag gctcgtagcc gagaaaggat atgagcagcg accggatgcc
     1381 cctcttcttc aggtaaggga agagacccga taggacgctg tcggcaaact cactcgtcat
     1441 gtggccgctc ttgaaatcgt agaaactgtg atagcacccc ttgcacgaga ggttgcattt
     1501 ggcggagaga agaaaggtca ccttggatat cctggcgacc tcgttcctgt gccttgccat
     1561 ggcgacatac tccgcgaaca tgtcccgctc gtccctgtct ccctccacca ggaaacgttt
     1621 ctcgtagagc tccctcatga ggaccgaagg ctcgcaccgg cacatcgccg acacttgcgc
     1681 cgcggccgtg cccatcgaga cgggcctgtc gaaggctccg agaacgtcga tgatctcaga
     1741 cccgtatgac gtcaggttgc cgtgcagctt gctgtatacc cttacctcgt catcgcgcag
     1801 ttgccttacc accagaaagg gcgacttctt caacataaca tcgggcggca tttctatcgg
     1861 gttcacgtga ttttctccca tattcagtgc ggttcgtcag gaactcgggc cctatctttt
     1921 tttgtcgatc tgatgtcctg tcattatggt aggttcacca taaaaaccac cgtccttctc
     1981 tgtcaagata caccagatca ggcgcaatac tcaagcaccg ctcgcggata gcgacccggc
     2041 gtgcctcatg cctcgatgga ccggtgacgc ggcatgtgca ctattgttgt tttcttcaag
     2101 aactcaatct gctagaatcc ttaacagtac gatccaaaca agcggggtga cgcggtggaa
     2161 gcattgaaag attggttgag gcccgagttg atatggtttc tcatcggcct tgcgctgatg
     2221 ctcctggagt tcgtccttcc aggcttcatc atattcttct tcggcgtcgg ggcctgggtc
     2281 gtggcgctca tctgcctctt cgccgacgta tccatcaacg cccaactcat cgtgttcctc
     2341 gtggcctcgg tgctgcttct cctctcgttg aggaaatgga tgaggggcgt cttcgtcggc
     2401 cgcaagaagt tcggccggcc atctgacgac gaccagttgg aattcacggg aaagagggcc
     2461 gtggtgacac ggacgatcga ccccgtcatg ggaggcaagg tcgagcttca cggcaccagt
     2521 tggaacgcgg agaccgatgg ggacgaaccg gttgaagaag gggccgccgt ggagatcaca
     2581 ggcaagcagg gccttacgct gaaggtcaaa aaatatccgg caaagggagg gaacggtcaa
     2641 tgaacttctt tactgtcatc atcgtcggtc tcatcatact cgtggtcatc gcctttctga
     2701 agaccatacg cgttgtcccc cagaaggtcg ccttcatcgt ggagcgcctc ggcaagtaca
     2761 acgcgacgct ggacgccggc ctccacgtcc tcataccctt tatcgacaag gtctcctaca
     2821 gacacaccct gaaggaacag gccatcgacg tcccctccca gacatgcatc acgagggaca
     2881 acatcgccgt cgaggtcgac ggcatcctct accttcaggt catagatccc cagaag
//
LOCUS       DAOQTR010000280         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_39640_length_2934_cov_4.095172, whole genome shotgun sequence.
ACCESSION   DAOQTR010000280 DAOQTR010000000
VERSION     DAOQTR010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39640_length_2934_cov_4.095172"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /gene="galT"
                     /locus_tag="PKN85_07335"
     CDS             complement(<1..717)
                     /gene="galT"
                     /locus_tag="PKN85_07335"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNT44260.1"
                     /translation="MPELRKDPIIDRWVIISMERGKRPVFFAEEAPPTKAAVCPLCPG
                     NENMTPPEVYVVRPPGSTPGSPDWSLRVVPNKFPALRIEGTLNREGIGIYDKMNGIGA
                     HEVIVETPNHGETLSDMPVERVQSVFIAYRERMRDLMRDTRFKYIMVFKNSGSVAGAS
                     LDHAHSQLIALPIVPRRVSEEVGGGLAYYKYKERCIFCDIIAQEKEENTRIVCENERY
                     VAISPYASRFPFETWILPKRH"
     gene            778..1464
                     /locus_tag="PKN85_07340"
     CDS             778..1464
                     /locus_tag="PKN85_07340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNT44261.1"
                     /translation="MIPLKDNIPTRTFPIITMGLIFVNIIIFLWQHLALHGPASALLY
                     TYYGLVPREFIASVTARHDLLPYNIMTVFTSMFLHGGFLHIIGNMLYLWIFGNNVEDR
                     LGHMGYLLFYLLSGVCAAVFQIFYDPSSEVPMIGASGAVSGILGAYLLVYPHARIKTL
                     LIIIIFIKVVDIPAIVLLTIWFFMQFLYSNVDGIAWYAHVGGFIFGLLFALLFLVRSK
                     GRKAGRASGA"
     gene            1461..2780
                     /locus_tag="PKN85_07345"
     CDS             1461..2780
                     /locus_tag="PKN85_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNT44262.1"
                     /translation="MRVPRVLAINPYIYDFAAYNFWSSPLGLLYIAAILRRNGFHVDL
                     IDCMSVSEEKRKPDGRAPFRKHKVPKPDALKGVRKTLRRYGMPPEELSALLEGMEPPD
                     IILVTSIMTYWYVGAMEVVDLARRVHPRSKIVVGGIYPTLCREQASLRFAAADLVVSS
                     GELGDFYGYVEAATGRRPGFTPHMDNLDILPYPAHDLCRDIPFVPLLTSLGCAFRCTY
                     CATSYMYPAMARRGVDSVVDEILHWHDRGVRRFVVYDDSFLFDSSRHAKPLLERIAGL
                     PFPVDIYNPNALNAAFIDDEVANLLVSSGFREVRIGLESVDPGRQAQTGGKVTGALFE
                     RAVGALRRAGYGRGQIRAYILAGLPGQPWQEVRRAIDYLKALDVTPSIAEYTPIPHTP
                     LFDKHKDSARFPVADDAIFQNNALFPYAWEGFTEEDLDYLKKYAKKG"
BASE COUNT          561 a          927 c          802 g          644 t
ORIGIN      
        1 gtgcctcttc ggaaggatcc atgtctcgaa gggaaagcgc gaggcatagg gtgatatcgc
       61 gacgtatctc tcgttctcgc agacgatgcg agtgttctcc tctttttcct gggctatgat
      121 gtcgcagaag atgcaccgct ccttgtactt gtaatacgcg agaccccctc ccacctcctc
      181 cgatacccgc ctcggcacga tgggcagggc gatgagctgg gaatgggcgt ggtcgaggga
      241 cgcccccgcc acggacccgc tgttcttaaa gaccattata tacttgaacc gggtgtccct
      301 catgaggtcc cgcatccgct cgcgatatgc tatgaagacg ctctggaccc tctccacggg
      361 catgtccgaa agggtctccc cgtgatttgg agtctccacg atgacctcat gggcgccgat
      421 accgttcatc ttgtcgtaga ttcctatccc ttcccggttg agggtgccct ctatcctcag
      481 ggcggggaac ttgttgggga ccaccctgag cgaccagtcg ggagaccccg gtgttgagcc
      541 cggcgggcgg acgacgtaca cctcgggagg cgtcatgttc tcattacccg ggcacagggg
      601 gcagaccgcc gccttcgtcg gcggcgcctc ctccgcaaag aagacggggc gcttgcccct
      661 ctccatagaa atgatcaccc atcggtcaat gatgggatct tttctcagtt caggcatacc
      721 ttactccgga tcctcgtttt ttgacatgac tgttgttgcg tgataagaaa gagttcagtg
      781 ataccgctga aggacaatat cccaacccgc acctttccca ttataacaat gggtctgatc
      841 tttgtaaata tcattatttt cctgtggcag catctggcgc tccacgggcc ggcgagcgca
      901 ctgctctaca cttactacgg tctcgtgccc agggagttca tcgcctccgt cacagcgcgg
      961 cacgacctgt tgccctacaa catcatgacg gtcttcacgt caatgttcct tcacggcggt
     1021 ttcctccata ttatcggcaa catgctctat ctttggatat tcgggaacaa cgtcgaggac
     1081 aggctggggc acatgggata cctcctgttc tacctccttt ccggggtatg cgcggcggtc
     1141 ttccagatat tctacgatcc ttcgtcggag gtccccatga taggggccag cggggccgtc
     1201 tcgggcatac tcggggcgta tcttctcgtc tatcctcatg cccggataaa aacgctcctc
     1261 atcataataa ttttcatcaa ggtggtcgac attcccgcca tcgtgctgct gaccatctgg
     1321 ttcttcatgc agttcctcta ctccaatgtc gacggcatcg cctggtacgc ccatgtgggc
     1381 ggcttcattt tcggcctcct cttcgccctt ctcttcctcg tccgcagcaa gggccgcaag
     1441 gcgggcaggg cctccggcgc atgagagtcc caagggtcct cgccatcaat ccctatatct
     1501 acgattttgc cgcctacaat ttctggtcga gccctctcgg cctcctttat attgcggcca
     1561 tcctgaggcg caacggattt cacgtggatc tcatcgactg catgtccgtc agcgaagaga
     1621 agcgcaaacc tgacgggcgc gcgcccttcc gcaaacacaa ggtgccgaaa cccgacgcgc
     1681 tcaagggggt gagaaagacc ctcaggaggt acgggatgcc acccgaagag ctgtcggccc
     1741 ttctcgaagg catggaaccg ccggacatca tcctcgtaac ctccatcatg acctactggt
     1801 acgtgggcgc gatggaggtc gtcgatctcg cacgccgggt acacccccgg tcgaagatag
     1861 tcgttggcgg catatacccc accctgtgcc gggagcaggc ctccctgagg ttcgcggcgg
     1921 cggacctcgt cgtttcttcc ggggagctgg gggacttcta cgggtacgtc gaagccgcga
     1981 cgggacgacg gcccggcttc accccccaca tggacaacct cgacatcctg ccctatcccg
     2041 cacacgacct ctgccgggac atccctttcg tgcccctgct gacgtcgctc gggtgcgcgt
     2101 tccgctgcac ctactgcgcc acgtcctaca tgtatccggc aatggcgcgg cgcggcgtcg
     2161 attccgttgt ggacgagata cttcactggc acgacagggg cgtccgccgc ttcgtcgtct
     2221 atgacgacag cttccttttc gacagttcgc gtcacgcaaa accccttctc gagaggatcg
     2281 ccggccttcc cttccctgtg gacatataca accccaacgc cctcaacgcc gccttcatag
     2341 acgacgaggt ggccaacctg cttgtcagct cgggctttcg cgaggtccgg atcggactgg
     2401 aatcggtcga tcccggcagg caggcacaga ccgggggaaa ggtgacgggc gccctcttcg
     2461 aaagggctgt aggcgccctc aggcgggcgg ggtacggcag gggccagatc agggcctata
     2521 tcctcgccgg cctcccggga cagccctggc aggaggtgag gcgggccatc gattatctca
     2581 aggccctcga cgtcacacca tccattgccg aatacacgcc gatcccccac acgccgctct
     2641 tcgacaagca caaggattcc gccaggtttc ccgtggccga cgacgccatc ttccagaaca
     2701 acgcgctctt cccctatgcc tgggaaggct ttacggaaga ggacctcgat tatttgaaga
     2761 aatatgcaaa aaaggggtaa tgggtgatag gttgtgggtt atgggaagaa ctcgcaaaaa
     2821 gatgtccctg ttgcccatga cccatgaccc atgacctgca tttaggacag atatttgtgc
     2881 agcttctggt aactcaggaa atcgccgtct atgaagtccg ccccgagttc cttc
//
LOCUS       DAOQTR010000281         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_40095_length_2913_cov_3.832050, whole genome shotgun sequence.
ACCESSION   DAOQTR010000281 DAOQTR010000000
VERSION     DAOQTR010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40095_length_2913_cov_3.832050"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1011)
                     /locus_tag="PKN85_07350"
     CDS             complement(10..1011)
                     /locus_tag="PKN85_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT44263.1"
                     /translation="MDEKTDEGLLRLSFDDINVARSLFGPREENIKYLRKYFNIKASI
                     RGNHLTLIGGKKDIEDTGKVINELHGIVKKGFPVNTSDIDHAVRFVVNAHETARDAQK
                     DQIYIAGSKKLITPKSTNQRQYVHAMNKYDVVIGIGPAGTGKTYLAMAMALAAYYRRD
                     VSRIILTRPAIEAGEKLGYLPGTMYEKVNPYLRPLYDALYDMVDMDRATRLIERGVIE
                     IAPLAFMRGRTLNDAFIILDEAQNTASEQMKMFLTRLGFSSKTVITGDITQIDLADKK
                     TSGLVEVRGILKGVKGIKFVYFTEKDVVRHPLVQKIIKAYEARRLELEESRNAERKG"
     gene            1181..1711
                     /locus_tag="PKN85_07355"
     CDS             1181..1711
                     /locus_tag="PKN85_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44264.1"
                     /translation="MPKKTLSFKTVLTMGVIIGIVVIAIAVIAIQFQQKRTLKASIEG
                     TLPRMEEKTGWQALTADGEGEHHYIIEKKQGESGQAVGAWDRLTYSKEGKEKYILKRR
                     RNGMFTTGMESLVRRFTLYDLKCGKEPRQYAIIKVFEVAEDGRTVDYGKAGSQKDWED
                     IPPEAIVDRLAKIACP"
     gene            1774..>2913
                     /gene="lpxC"
                     /locus_tag="PKN85_07360"
     CDS             1774..>2913
                     /gene="lpxC"
                     /locus_tag="PKN85_07360"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNT44265.1"
                     /translation="MNKVLIIDDERSILDSLSGILEDEGFSVLKATDGKEGLAIFERE
                     APDVVLLDVWMPELDGIQVLKEIRARKDDAKVVVISGHGTISTAVEAVKMGAYDFLEK
                     PLSIDKVLEVVSRSIGAGLKHDPSVADFRLEVAPGITREKQKTIGKSTVVYGVGLHSG
                     VKTGMILLPMPENTGIVFETIPDGERIPAYIDYVFSVGYASSVKGKNSVVRTIEHLLS
                     ACHMYGITNLLVKVSEEIPVFDGSAIEICRKIDEAEIVEQTDGIEPVVITEKVELPNL
                     QNGKYLAAEPSERFEIDYLLEQKRPIGTQHYRFVAGRDDYVANIAPARTFGFLKDFEQ
                     LAKMGLGTGGRINNFIILGEDEVINTKLRFEDEFVRHKILDLIGDI"
BASE COUNT          657 a          808 c          825 g          623 t
ORIGIN      
        1 gagggaggtt tatcctttcc gttctgcatt cctcgactcc tccaattcca ggcgacgtgc
       61 ctcgtaggcc ttgattatct tctgcaccag gggatggcgc acgacatcct tttcggtgaa
      121 atagacaaat ttgatcccct tgacgccctt caggatgccc ctgacctcca cgagaccgct
      181 cgtcttcttg tcggcaaggt cgatctgggt gatatcgccg gttatcaccg tcttcgatga
      241 gaaaccaagc ctcgtcagga acatcttcat ctgctccgag gcggtgttct gggcctcgtc
      301 gaggatgatg aaggcgtcgt tgagggtcct gccgcgcatg aacgccaggg gcgcgatctc
      361 gatgaccccc ctttctatga gccgtgtggc ccggtccatg tcgaccatgt cgtagagggc
      421 gtcatagagg ggcctcaggt agggattgac cttctcgtac atcgtacccg gaagatatcc
      481 cagcttctcg ccggcctcta tagccggacg ggtgagaatg atcctggaaa cgtcgcgtct
      541 gtaataggcg gcaagggcca tcgccattgc aaggtacgtt ttccccgtac cggccgggcc
      601 gatgcctatc acgacgtcat acttgttcat agcgtggaca tactgtctct gattggtgct
      661 cttgggcgtg atgagcttct tcgagccggc gatgtatatc tggtccttct gtgcatcacg
      721 ggccgtctcg tgggcattaa cgacaaacct cacggcgtgg tcgatgtcgg aggtgttcac
      781 gggaaaacct ttcttcacga tgccgtgaag ctcgtttatg acctttcccg tatcctcaat
      841 atccttcttg ccccctatga gggtaagatg attgcccctg atgctcgcct tgatattgaa
      901 gtatttcctg aggtacttga tgttctcttc acgcggcccg aagagacttc ttgccacgtt
      961 tatatcatcg aaggacagtc gtaacaatcc ttcatcggtc ttctcgtcca acaatcctct
     1021 ctttcccctg ttttgttccc taatatatca taaaacagga ttttgcaaaa gatgcaagaa
     1081 ttttggcggc cgctcttgca aagggccctg ttcgatggta tggtttggaa aaaatcgccg
     1141 gagggcctgc gagcggcacg ctctcggaaa ggaatgtcat ttgcccaaaa aaacgctatc
     1201 cttcaagacg gtattgacga tgggggtcat catcgggata gtggtgatcg ccatcgccgt
     1261 catagccatc caatttcaac agaaaaggac cctcaaggcc tcgatcgagg gcacccttcc
     1321 aagaatggaa gagaagaccg gctggcaggc actcaccgcc gacggggagg gtgagcacca
     1381 ctacatcatc gagaaaaagc agggagaatc cggccaggct gtcggtgcgt gggacaggct
     1441 gacgtactcg aaggaaggca aagagaagta catcctcaag cggcgaagga acggcatgtt
     1501 cacaacgggg atggaaagcc ttgtccgccg cttcacgctc tacgacctga agtgcggcaa
     1561 agaaccccgt caatacgcca tcataaaggt cttcgaggtg gctgaggacg gcagaacggt
     1621 ggactacgga aaggcgggaa gtcagaagga ctgggaggac atcccccctg aggccatagt
     1681 cgacaggctg gcaaagatcg cctgcccgta gcggacaccc ctcacgtcac aagtatttgt
     1741 aagtcgccgg catttcgtat agaatattgc gtcatgaaca aggtcctcat catagatgac
     1801 gaacggagca tcctcgactc gctctcgggg atcctcgagg acgaaggatt ctcggttctg
     1861 aaggccacgg acggcaagga aggccttgcc atcttcgagc gcgaagcccc cgacgtggtg
     1921 ctcctcgatg tctggatgcc tgagcttgac ggtatacagg tcctcaagga gatacgcgcc
     1981 cggaaggacg acgcgaaggt ggtggtcata tcggggcacg ggacgatatc gacggcggtg
     2041 gaggccgtca agatgggtgc ttacgacttt ctcgaaaaac ccctctccat cgacaaggtg
     2101 ctcgaggtcg tctcccgcag catcggcgcg ggtttgaaac atgacccctc tgttgccgat
     2161 ttcaggctgg aagtggcgcc cggcatcacg agggaaaagc agaagaccat cggaaagagc
     2221 accgtcgtct acggggtggg actccactcc ggcgtgaaga cgggaatgat cctcctgccc
     2281 atgccggaga atacgggcat agtcttcgag accattcccg acggggagcg tatacccgcg
     2341 tacatcgact acgtcttctc cgtggggtac gcgtcgtcag tgaaaggcaa gaacagtgtc
     2401 gtgcgcacca tcgagcatct cctgtccgcc tgtcacatgt acgggatcac caatcttctc
     2461 gtcaaggtga gcgaagagat acccgtcttc gacggttcgg ccatcgagat ctgcaggaag
     2521 atcgatgagg ctgagatcgt cgaacagacc gatggtatcg aaccggtcgt catcacggaa
     2581 aaggtggagc tccccaatct gcagaacggg aaatacctag cggcggagcc ctcggagcgc
     2641 ttcgagatcg actacctcct ggaacagaag aggcccatcg ggacccagca ttaccggttt
     2701 gtcgccggcc gcgacgacta cgtcgccaac atcgctcccg cgaggacctt cggcttcctc
     2761 aaggactttg aacagcttgc caagatgggt ctcggaaccg gcgggcgcat caacaatttc
     2821 atcatcctcg gcgaagacga ggtcataaac acgaagctga ggtttgagga cgagttcgtc
     2881 cgccacaaga tcctcgacct catcggcgac atc
//
LOCUS       DAOQTR010000282         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_40237_length_2906_cov_3.164504, whole genome shotgun sequence.
ACCESSION   DAOQTR010000282 DAOQTR010000000
VERSION     DAOQTR010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40237_length_2906_cov_3.164504"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKN85_07365"
     CDS             <1..705
                     /locus_tag="PKN85_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNT44266.1"
                     /translation="LYVVNGSKVFTTNAGYADFYALLAKDEDRQSIMIIDRELIEKNG
                     PGRFEINDLGRKMGINTTTSGELVFSDFEIPKNNVLGQRGKGLRMVLDGFELGRIVIA
                     AQAFGAMLYGYEKTVGYAKKRKQFGQSIIRFQSVEHDLVDMYVEIEKCRNLLYKAAWL
                     YDNKDPESKKYSSLVKLAIPECAIKVLNRTIDILGGYGYMRDQGLEGALRDIRITSIY
                     EGTSNIQKNTLSRMIF"
     gene            complement(834..2690)
                     /locus_tag="PKN85_07370"
     CDS             complement(834..2690)
                     /locus_tag="PKN85_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNT44267.1"
                     /translation="MLIVNNLSKAYGTQTLFDGASFTVGPGERLGLVGRNGTGKTTLF
                     RMILGEETPDSGEIHIPRGYTIRHLSQHISFSERSVLAEACLNLPVSDDGRDETYKAK
                     TVLAGLGFTEDEFSSDPEKLSGGFQVRLNLAKILIARPMMLLLDEPTNYLDIVSIRWL
                     TQFLKGWKGEMVLITHDRDFMDSVTTHTMAIHRMRMRKIAGTTHKLYEQIIEEEEVYE
                     QTRVNEEKKRKEIELFINRFRAQATRARSVQSRVRQLQKKERMEKLATLENLEFSFNA
                     APFPGRWLLEADNVGFSYDPSGPELISDLTIAVRKRDRIGIIGKNGKGKTTLLNILAG
                     EFTPRSGTITYSQNLKIGYFGQMNIDRLDPEVTIEDEIWSVEPGRGKTAVRGICGAML
                     FDGDKALKKIGVLSGGERSRVLLGKLLAAPANLLLLDEPTNHLDMESIDSLIEAINEF
                     DGAVMIVTHSEMILDAVAERLIVFDGNRVGIFDGSYEDFLSRVGWEDEAEGRAGESEG
                     VAAPAKSAIRRESKRLKADIVNERSRAMTPVKTRINDIESAITETERQLARDNEALIA
                     ASQKGDGKAIISLSMSIHEARQRIDGLFDELEVLTLEHDALAREFEQRLNEL"
BASE COUNT          559 a          871 c          867 g          609 t
ORIGIN      
        1 ctctatgtgg tgaacggctc gaaggtcttc acgacgaacg cggggtacgc cgacttctac
       61 gccctcctcg cgaaggacga ggaccgtcag agcataatga tcatcgacag ggaacttatc
      121 gagaagaacg gtccgggccg gttcgagatc aacgacctcg gcaggaagat gggcataaac
      181 accaccacgt cgggagaact cgtcttcagc gatttcgaga tcccgaaaaa caacgtcctc
      241 ggccagcgcg gcaagggctt gaggatggtc ctcgacgggt tcgaactggg caggatagtt
      301 atagccgccc aggccttcgg ggccatgctc tacggctacg agaagaccgt cggctacgcg
      361 aagaaacgca agcagttcgg ccagtccata atccgttttc agtccgtcga gcacgacctc
      421 gtggacatgt acgtggagat cgaaaagtgc cgcaacctcc tgtacaaggc cgcctggctc
      481 tacgacaaca aggacccgga atcgaagaag tactcctccc tcgtgaagct ggcgataccg
      541 gaatgcgcca taaaggtcct caacaggacc atcgacatcc tgggaggcta cggctacatg
      601 cgcgaccagg gcctcgaggg cgccctgagg gacatacgga taacatcgat ctacgaaggg
      661 acgagcaaca tacagaagaa cacactgtcg agaatgatct tctaaaggac ggttcaaggg
      721 ttcaagggta agaccgcgaa gggaggtcgg ctgtcaacac aattcgcaca ccggccgaag
      781 gcaccggtgt cctgtttgtc ttttactctt gaacccttga accttcttca tctctacagt
      841 tcgttcagcc gctgttcgaa ctcccgtgcc agggcgtcgt gttccagggt gaggacctcg
      901 agttcgtcga agaggccgtc tatccgctgt ctggcctcgt ggatggacat cgagagggat
      961 atgatggcct tgccgtcgcc cttctgtgag gccgcgatga gggcctcgtt atcgcgggcg
     1021 agctgcctct ctgtttcggt gatggcggac tcgatgtcgt tgatgcgcgt cttcacgggc
     1081 gtcatggcgc gggaacgctc gttgacgatg tcggccttga gccttttcga ttctcttctg
     1141 atggcgctct tcgccggggc cgcgacgccc tcgctctccc ctgccctgcc ctcggcctcg
     1201 tcctcccagc cgacgcggga gaggaagtcc tcgtagctgc cgtcgaagat gccgaccctg
     1261 ttcccgtcga agacgatgag ccgctccgcg acggcgtcga ggatcatctc gctgtgggtc
     1321 acgatcatca ccgcgccgtc gaattcgttg atggcctcga tgagggagtc gatggactcc
     1381 atgtcgagat ggtttgtggg ctcgtcaaga agcaggaggt tcgccggggc ggcgaggagc
     1441 ttgcccagga gcaccctgct cctctcgccc ccggagagca cacctatctt cttgagggcc
     1501 ttgtccccat caaagagcat ggcgccgcag atacccctga cggccgtctt gccccggccg
     1561 ggttccacgc tccatatctc gtcctcgatg gtcacctccg ggtcgaggcg gtcgatgttc
     1621 atctgcccga aatagccgat cttcaggttc tgactgtatg tgatcgtacc cgagcggggc
     1681 gtgaactccc cggcaaggat gttaagaagc gtcgtcttgc ccttcccgtt cttcccgatg
     1741 atcccgatgc gatcgcgttt tctgacggct atggtgaggt cactgatgag ctccggcccc
     1801 gacgggtcgt aggaaaaacc cacattgtcc gcctcgagga gccatctgcc ggggaacggg
     1861 gcggcattga aggagaactc gaggttctcg agggtggcaa gcttctccat cctctccttc
     1921 ttctggagct ggcggaccct ggactgcacg gacctcgccc gcgtggcctg ggcgcggaag
     1981 cggttgatga aaagttctat ctctttccgc ttcttctctt cgttgacgcg ggtctgctcg
     2041 tagacctcct cttcctcgat gatctgctcg tatagcttgt gggtcgtccc cgcgatcttg
     2101 cgcatcctca tacggtggat ggccatggtg tgggtcgtca cggagtccat gaaatccctg
     2161 tcgtgggtga tgaggaccat ctcgcccttc caccctttca ggaactgcgt gagccagcgt
     2221 atcgagacga tatcgagata gttcgtcggt tcgtcgagaa ggagcatcat cggccgggcg
     2281 atgaggatct tcgccaggtt gaggcgcacc tggaaaccgc cgctcagttt ctccgggtcc
     2341 gaagagaact cgtcctcggt gaagccgagc ccggcgagca ccgtcttcgc cttgtacgtc
     2401 tcgtcgcggc cgtcgtcgct cacgggaagg ttgaggcacg cctcggcgag gacggacctt
     2461 tcgctgaaag agatgtgctg ggagagatgg cggatggtgt agccccgggg gatatggatc
     2521 tcccccgaat cgggcgtctc ttccccgagt atcatcctga agagcgtcgt cttccccgtc
     2581 ccgttccgcc ccacgagccc gaggcgctcc cccggcccga cggtaaagct cgccccgtcg
     2641 aagagtgtct gggtcccgta ggccttggaa aggttattga cgataagcat ggcagcgccc
     2701 gcaaagcgct ctatattaac ccaacagaag ggatggttgc aacagcttga acagcttgaa
     2761 cgctcgaacg gaagacatct gagaccgcag actgttcgaa ccgcccgaga ccgccgcaga
     2821 aacggcaaaa ggattcttcc tccctttcgc gctcaagcgg ttcaagcggt ttctacgggt
     2881 tttcttccat ctcccatatg ccgcag
//
LOCUS       DAOQTR010000283         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_40249_length_2905_cov_3.882105, whole genome shotgun sequence.
ACCESSION   DAOQTR010000283 DAOQTR010000000
VERSION     DAOQTR010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40249_length_2905_cov_3.882105"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..973)
                     /locus_tag="PKN85_07375"
     CDS             complement(131..973)
                     /locus_tag="PKN85_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44268.1"
                     /translation="MALMEVRRVTFAYKDKPVLKDVSVEIGEGEVVTLLGPNGSGKST
                     LLKTIIGLIRPGQGTVRLAGRDISSIPQKELARQIAYVPQFHRSAFPYRVFDVILMGR
                     MPHKTFFFRYSQEDIDIARTVMDRLSIAHLAGRPYTEISGGERQLTLIARALAQGARV
                     FVMDEPASGLDYGNQLKLLEHITDLSGEGYTFIKSTHSPEHALWIAHRAVMIRKGAII
                     ADGCCDDVVNSETLLQLYNTRVKVVRLNGSLRVCVPEMLKDNGAEEGTGRQPMFVRSG
                     TSRR"
     gene            complement(973..1986)
                     /locus_tag="PKN85_07380"
     CDS             complement(973..1986)
                     /locus_tag="PKN85_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNT44269.1"
                     /translation="MRTGFVITGLACALVVAVLVSLVLGKYPVGIDDVCGFFLWKAFN
                     ASVNLSIDRQLLENILLNIRLPRIMAALFVGASLSISGAAFQAMFINPLVSPGILGVL
                     AGASFGAAVGMVLSKSWFFVQASCFLFGFLAVAAAVGVARLYKGNSIILLILGGVISG
                     ALFTSLLSIVKYLADPYNQLPAIVYWLMGGLTLVDAGVIRNTAIPVVAGVGLVISLSG
                     YLNVLSMGDEEARSMGVPVERIRIILIVIATMLSAITVVLAGMVGWVGLIIPHISRMI
                     VGPDNRVLIPVSALVGALYLVAVDDVSRLLFTTEIPLGIITSLVGIPFFAVVLKRAGK
                     GWS"
     gene            complement(2012..2821)
                     /locus_tag="PKN85_07385"
     CDS             complement(2012..2821)
                     /locus_tag="PKN85_07385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT44270.1"
                     /translation="MRDTAIDWNDVWKKESAKSSVKKRDVTFWNEKARNFSKKPWESN
                     YSEDFLKIMEPRRCWTVLDMACGTGALTIPLARYVRRITAVDFSPRMLEVLEEQCRVE
                     GIENVRTINASWEDDWSSRGIDEHDVVIASRCLVVDDLRKAVMKLNGAARERVYISTI
                     VGDGPHDRMIYEAVGRRLTPGPDYIYTYNLLHQLGIYANLFIIKDIRTESFRDNDEAL
                     AYYEHFLGKLTKGEKEDLRKYLQRHLVPKNGRWVLDYRRTTRWAVMWWDKE"
BASE COUNT          582 a          874 c          864 g          585 t
ORIGIN      
        1 ggccccgaga aggaaatgga gcggtgcgca cgcgcggccg gtgcttttta ggatggaccg
       61 tcttaccggc ctgaggacgt gcacgttctc caacaccgtt tccagcgcct gctcgtaact
      121 gatcattgcc tcacctccgc gaagttcccg aacgaacgaa catgggctgg cgccctgttc
      181 cctcctcggc gccgttgtcc ttcagcatct cgggcacaca gacgcgcagc gatccgttca
      241 atctgaccac cttgaccctg gtgttataaa gctgcagcag ggtttcgctg ttcacaacat
      301 cgtcgcagca cccgtcggcg atgatcgccc ccttccggat cataacggcg cggtgtgcta
      361 tccagagggc gtgctccggt gagtgggtcg actttatgaa ggtgtatccc tccccggaca
      421 ggtccgtgat gtgttcgagc aatttgagct gattgccgta gtcgaggccg ctcgcgggct
      481 cgtccatgac aaagacgcgg gcgccctgcg ccagggcccg ggcaatgagg gtgagctgtc
      541 tctcgccccc gcttatctcc gtgtagggac gcccggcgag atgcgcgatg gagagtctat
      601 ccataacggt ccgcgcgatg tcgatgtctt cctgggagta ccggaagaag aacgtcttgt
      661 ggggcatgcg gcccatgagg atcacatcga agaccctgta gggaaaggcg ctcctgtgga
      721 actgggggac gtaggctatc tgcctggcca attccttctg cggaatggac gagatgtccc
      781 gtcccgcgag gcggacggta ccctgaccgg gcctgatgag gccgatgatc gtcttgagga
      841 gggtgctttt cccactcccg ttgggaccga ggagagtgac cacctctccc tcgccgatct
      901 ccacgctgac gtccttcagg accggtttgt ccttgtacgc gaaggtgacg cgcctgacct
      961 ccatgagagc catcaactcc acccctttcc ggccctcttg aggaccacgg cgaagaaagg
     1021 tatgccgacg agggaggtga tgatccccag gggtatctcc gttgtgaaga gaaggcgcga
     1081 aacgtcatcg acggcaacga ggtaaagcgc cccgacgagg gcgctcaccg ggatgagcac
     1141 gcggttgtcc ggtcctacga tcatcctcga gatgtggggt atgatgagcc cgacccagcc
     1201 gaccatcccg gcaagcacga cggtgatggc gctcagcatc gtggcaatga caatgagaat
     1261 gatccggatc cgttcgacgg gaacgcccat ggaccgcgcc tcctcgtcac ccatggagag
     1321 gacgttgagg taccccgaaa gcgagatgac gagaccgaca ccggcaacga cggggatggc
     1381 cgtgttcctg atgacgcccg cgtcaacaag ggtgaggccg cccattagcc agtagactat
     1441 cgcgggaagc tggttgtaag ggtcggcgag gtacttcacg atggacaaaa gggaagtaaa
     1501 aagcgcaccg cttattaccc cgccgaggat caggaggatg atcgagttgc ccttgtaaag
     1561 gcgggcaacg ccgaccgccg ccgctacggc cagaaaaccg aagagaaaac agcttgcctg
     1621 cacgaagaac cagctcttcg agagcaccat cccgacggca gcgccgaagg acgctcccgc
     1681 gagcacgccc aggatgcccg gtgagacaag agggttgatg aacatcgctt ggaaggcggc
     1741 ccccgatatg gacagcgatg ctcccacgaa gagcgccgcc atgatgcggg gcaggcggat
     1801 attgaggagg atgttctcca gcagctgcct gtcgatggac aggttcacac tggcattgaa
     1861 cgccttccaa aggaagaacc cgcagacatc atcgataccc accgggtact tgccgagcac
     1921 cagcgagaca agcacggcaa caacgagggc acaggcgagt ccggtgatga cgaatcccgt
     1981 tctcatggca ggaaggcctc gctcatctct ttcattcctt gtcccaccac atcaccgccc
     2041 atcgcgttgt cctcctgtaa tcgaggaccc acctcccgtt cttcggtacg aggtgtctct
     2101 gcagatactt cctgaggtcc tccttttcgc ctttcgtcaa tttcccgagg aaatgctcgt
     2161 agtacgcaag ggcctcatcg ttgtcacgga aggattcggt ccggatgtcc ttgatgatga
     2221 acaggttggc atagataccg agctgatgga ggaggttgta ggtgtatata tagtccggcc
     2281 cgggagtgag tcgcctcccg acagcctcat agatcatccg gtcatgaggc ccgtccccga
     2341 caatggtgga aatgtagacg cgttcccggg ccgcaccgtt caatttcatg acggctttcc
     2401 ggaggtcgtc gacgacgaga cagcgcgacg cgatcaccac gtcgtgttca tcgatgcccc
     2461 gggagctcca gtcgtcctcc cagcttgcgt tgatggtcct gacgttctcg atgccctcca
     2521 cccggcattg ctcctcgagg acttcgagca tccggggtga gaagtcgacg gcggttatcc
     2581 tcctgacgta ccgtgccagg gggatggtca gcgcgcctgt cccgcaggcc atgtccagca
     2641 cggtccaaca gcgcctgggt tccattatct tgaggaaatc ctcggagtag ttcgattccc
     2701 agggcttctt ggagaagttg cgggccttct cgttccagaa ggtgacatcc cgttttttca
     2761 cggaactttt cgcgctctcc ttcttccaga catcattcca gtcaattgcc gtatctctca
     2821 tcggactatc ctgtcttcac gtcttcttcc gccagtaggt tttgccttcc gcgcagggct
     2881 tgcagaggat cctgccgttg tcgcg
//
LOCUS       DAOQTR010000284         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_40285_length_2903_cov_4.393258, whole genome shotgun sequence.
ACCESSION   DAOQTR010000284 DAOQTR010000000
VERSION     DAOQTR010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40285_length_2903_cov_4.393258"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKN85_07390"
     CDS             <1..430
                     /locus_tag="PKN85_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNT44271.1"
                     /translation="GYFRWDPDRGNAEDGFNGLLSLTQRDRLTTYTLILESGYREDLF
                     SADNLGFTQYYRASAVVNHRLTERFSVGLVGIVERTDYSQNPRKDWLYTADLTADYRV
                     LRWLTIGARVGYAEDDSNIPASSYDEWHAFLTLTASYNVF"
     gene            492..1913
                     /locus_tag="PKN85_07395"
     CDS             492..1913
                     /locus_tag="PKN85_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNT44272.1"
                     /translation="MINPGRSYNLEDYKEIFLRRRLYFIVPLVVIFLGACLVAAFTPR
                     KYMASTLVLVTPPRIPAELVRSTVTSGITERLNSISQEIMSRTRLEAVIDELKLFPEE
                     AKKMDREGLVALMRQNIKVDIPQRSSENVGYFAIKYTSKDPVVAAAVANKLSSLFIEE
                     NLKQREQQAVGTSEFLSNELKTTKERLEAQGKELADYKRRYLGELPEQRDTNIRLLEQ
                     LQQLYQRNEESLRAAQDRQLLIGRQIRDLENPSVQPGEGTSGTRSRGSFETQLDEMKK
                     NLAELQARYTERHPDVVSLKRKISEMEKRKDAFNIKNDPRYRELSNTMAATDMEIKRL
                     QDESGKLRAQIGQYRSRIEGATVREQQMSSLVQEYENTKRLYESLLKKSEEAQQAENL
                     ERRQKGEQFRVVDPARVPEKPFQPDVPKILLMGLIAGIACGLGGIFAREQLDRSFHDP
                     EDVEATLGFKVLANIPKIISRAS"
     gene            1916..>2903
                     /locus_tag="PKN85_07400"
     CDS             1916..>2903
                     /locus_tag="PKN85_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44273.1"
                     /translation="MYNKFFGLKDKPFEITPDPRYLYLSESHREALAHLTYALNESKG
                     FTVITGEVGTGKTTLVQMLLSRLDSHTRIAHLFNPKLGTRDFFKYICQDFGLKTDGLG
                     TKGEFLALLHTFLMECYARRERVVLIVDEAQTLSPALLEEVRLLTNLETPKAKLLQVV
                     LLGQPELDNTLARSEFRQLKQRISLRFNLKPLNRRETGEYIVRRLKAAGARNTDLFDD
                     DAVKEIYKYSKGIPRLINVVCDNSLMTGFVYEKHRIDRDIVREVIKDMEGPAERKGWK
                     AIILPAIVILALAGMLIAWWGDLSFGNRPYAGESAQKVFRSKIMKALEITSLE"
BASE COUNT          672 a          874 c          835 g          522 t
ORIGIN      
        1 cggttacttt cgatgggacc ccgaccgggg gaatgccgag gacgggttca acggattgct
       61 gagcctcaca cagagggacc ggctcacgac gtacaccctg atcctggaaa gcgggtaccg
      121 ggaggatctg ttctcggcgg acaacttggg cttcacgcag tactaccgtg cttcagccgt
      181 cgtgaaccat cgtttgacgg agcggttctc cgtgggactg gttggcatcg tcgaacgtac
      241 cgattactcc cagaatccgc gcaaggactg gctctacacg gcagacctca cggcggatta
      301 ccgcgttctg cgttggctta ccatcggtgc gcgggttggc tacgccgagg acgactcgaa
      361 tatcccggcg tcgagctacg acgagtggca cgccttcctg accctgacgg caagctacaa
      421 cgttttctaa ccccgactca aattcatcac tcataactca aaactgttca ttagatcagt
      481 ctggagcaca aatgatcaat cccggccgct cttacaatct ggaggactac aaggaaatct
      541 tcctgcgacg caggctttat ttcatcgttc ctcttgttgt catattcctc ggcgcctgtt
      601 tagtggcagc tttcactccg agaaaataca tggcttccac gctcgtcctt gtcacacctc
      661 cgcgcatccc ggcggaactg gtccggtcca ccgtgaccag cggcatcacc gagcgcctca
      721 actccatcag ccaggagatc atgagccgca cgcggctgga ggcggtcatc gacgagttga
      781 agcttttccc cgaagaagca aagaagatgg acagggaggg ccttgtcgct ttgatgcggc
      841 agaatatcaa ggtcgatatc ccgcagaggt catcggagaa cgtaggctac ttcgccatca
      901 agtacacctc gaaggacccg gtggttgcgg ctgcagtggc caacaaactc tcgtcgcttt
      961 tcatcgagga gaacctgaaa cagcgggagc agcaggcagt cggcacgtcg gagttccttt
     1021 cgaacgagct caagacaacc aaagaacgtc ttgaagccca gggaaaggaa ctggcggact
     1081 acaagcggag gtacttgggg gagctgccgg agcagaggga tacgaacatc cggctcctgg
     1141 agcagcttca acagctatac cagcgtaatg aggagagcct cagggcggca caggataggc
     1201 agcttctgat cgggcgacag attagagatt tggaaaatcc ctccgtgcaa cccggtgaag
     1261 gcacttcggg tactagatcc cgggggagtt tcgagactca actcgacgaa atgaagaaga
     1321 acctcgcgga gctgcaagcg cgctacacag aacgtcaccc cgacgtggtt tccctgaagc
     1381 gaaagatttc agaaatggaa aagcgcaagg acgccttcaa catcaagaac gacccccgct
     1441 atcgggagct ttcaaatacg atggcggcga cggacatgga aatcaaaagg ctccaggacg
     1501 aaagcggaaa gcttcgcgct caaatcggcc aataccgtag cagaatcgag ggggccaccg
     1561 tgcgggagca gcagatgtcg tccctcgtcc aggagtatga gaatacgaag aggctctacg
     1621 agtcgctctt gaagaaaagc gaggaggccc agcaggccga aaacctcgag cgccggcaga
     1681 agggcgagca gttccgcgtt gtcgacccgg cgcgggtgcc tgagaaaccg ttccaaccgg
     1741 atgttcccaa gatcctgctc atgggtctta ttgctggcat cgcctgcgga ctgggcggca
     1801 tcttcgccag ggagcagctc gaccgctctt tccacgatcc cgaggacgtg gaggccacgc
     1861 tgggcttcaa ggtgctcgcc aacatcccta aaatcatcag cagggcatcg taaacatgta
     1921 caacaaattc ttcggactga aggacaagcc cttcgagatc acgccggacc ccaggtacct
     1981 gtacctgagc gaaagccacc gcgaggccct ggcccacctg acctatgccc tgaacgaatc
     2041 gaagggcttc acggtcatca cgggcgaggt cggcacgggg aagacgaccc tcgtgcagat
     2101 gctcctctcc cggctcgaca gccacacgcg tatcgcccac ctgttcaacc cgaagctcgg
     2161 cacccgggac ttcttcaagt acatctgcca ggacttcggg ctcaaaacgg acgggctcgg
     2221 caccaaggga gaatttctcg ccctactgca taccttcctc atggaatgct acgcccgcag
     2281 ggagcgggtg gtcctcatcg tcgacgaggc ccagacgctc tcgcctgccc tgctagagga
     2341 ggtgcggcta ctcaccaatc tcgagacgcc gaaggccaag ctcctgcagg tcgttctcct
     2401 gggccagccc gagctggaca atacgcttgc ccggagcgaa ttccggcagc tcaagcagcg
     2461 gatctccctg cgcttcaatc tcaagcccct caaccgccgg gaaacggggg agtacatcgt
     2521 ccggcggctc aaggccgcgg gggcccggaa cacggatctc ttcgacgacg atgccgtcaa
     2581 ggagatctac aagtactcca aggggattcc tcggctcatc aacgtcgtct gcgacaacag
     2641 cctcatgacg ggcttcgtct acgagaagca ccgcatcgac cgggatatcg tccgcgaggt
     2701 catcaaggac atggaaggcc cggcagagcg caaggggtgg aaggccatca tcctgccggc
     2761 catcgtcatc ctcgccctgg ccggaatgct catcgcgtgg tggggcgatc tcagcttcgg
     2821 caatcgtccc tatgccggag agagcgccca gaaggtgttc cgcagcaaga tcatgaaggc
     2881 cctcgagatc acctccctcg agc
//
LOCUS       DAOQTR010000285         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_40358_length_2900_cov_4.437961, whole genome shotgun sequence.
ACCESSION   DAOQTR010000285 DAOQTR010000000
VERSION     DAOQTR010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40358_length_2900_cov_4.437961"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..633
                     /locus_tag="PKN85_07405"
     CDS             82..633
                     /locus_tag="PKN85_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT44274.1"
                     /translation="MLEVRFHGRGGQGAVTSAEIIAQAAIKQGMNAQAFPSFGPERRG
                     APVQAFLRVSDKPIRLRSKVYKPDSVLILDPTLITSANPTAGLKKGGYIIINSNKSVE
                     ELKNTFPGQNIIAVDASKIAREELGVPITNTTMLGALVRATRVVELSTLEEPVRNRFG
                     VNGQKNINAYTRAYNEATVIEAE"
     gene            754..2376
                     /gene="ade"
                     /locus_tag="PKN85_07410"
     CDS             754..2376
                     /gene="ade"
                     /locus_tag="PKN85_07410"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNT44275.1"
                     /translation="MKTISGNIVDLHNETVFPGTVHIANGIITGIDRERYWYDTYILP
                     GFVDSHVHVESSMLTPAGFARLAVVHGTVATVSDPHEIANVLGPQGVRFMMEDAAGIP
                     FVFAFGVPSCVPASPFETSGAAMGLAEMEALFDEQGAGYLSEMMNVPGVIGGDPDVQA
                     KIEAARRRGKPVDGHAPGLRGEGLRRYIAAGISTDHESFELEEAREKVSLGMKILVRE
                     GSAARNFDTLYPLIDESPGFCMLCTDDQHPDSLVKGHINTLVARAIRKGVDVMKVLRC
                     ACVNPVLHYGLDVGLLRPSDPADMIVVDGLEDLEVLKTIVRGEIVAEAGESRIPHRRA
                     TPVNIFRATVRKPEDFRVLKRSDAIRVIGVIEGQVVTYAHLEAPALEGDALACDTDRD
                     ILKIAVVNRYADRPPAVAFVRGFGLKKGAMASSIAHDSHNIVAVGTSDEMLCDAVNLV
                     IEKKGGISLVSEEMKMVLPLPVAGLMSNEDGYSVAERYSRLTLLARDLGTTLKAPFMT
                     LSFMALVVIPKLKLSDKGLFDGEKFTFTDLFV"
     gene            complement(2457..>2900)
                     /locus_tag="PKN85_07415"
     CDS             complement(2457..>2900)
                     /locus_tag="PKN85_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT44276.1"
                     /translation="LFKGLTEKRLKDLALLAQFHDIGNISIHDRILLKSGSLTEEELN
                     EMRKHCDIGHRIAQSAATLTPIADYILKHHEWWDGSGYPLGLTGEEIPLESRIMAIAD
                     AYESMTGGRPHRKAVSREEALKELRRCAGTQFDPDLVKKFIKIVA"
BASE COUNT          618 a          812 c          843 g          627 t
ORIGIN      
        1 acaaggggat catgctcctt gacacaccca aaggaacaca gtggcggata aagacttcag
       61 tttcttaaaa ggagagagta tatgttagag gtcagatttc acggaagagg cgggcaggga
      121 gccgttacgt ccgccgagat catcgcacag gctgccatca agcagggcat gaacgcgcag
      181 gcattcccga gcttcggtcc cgagcgccgc ggagcccctg tccaggcttt tttgcgtgta
      241 tccgacaagc ccatcaggct gaggtcaaag gtctacaaac ctgacagcgt tctcatcctg
      301 gaccccaccc tcatcacctc ggcaaacccg acggcaggtc tcaagaaggg tggttatatc
      361 atcatcaatt ccaacaagtc tgtggaagag ctgaaaaaca catttcccgg gcagaacatc
      421 attgccgtcg acgcgtcgaa gatcgccagg gaagagctgg gtgttcccat aacgaacaca
      481 acgatgctcg gggccctcgt tcgggcaacg cgcgtcgtcg agctcagcac gctggaagag
      541 ccggttcgca accgcttcgg cgtcaacggc cagaagaaca tcaacgccta caccagggca
      601 tacaacgaag cgacagtgat agaggcggag tgatatactt acaataacca atgaccaacc
      661 tccaatgacc agtcaagaga cagccggcgg caaaggtctt tcccctggtc actggaattt
      721 ggttattggt tattcgatac ttcgggtctt tcaatgaaga ccatctccgg taatatcgtc
      781 gatctccata acgagaccgt ctttcctgga acagtccata tcgccaacgg catcataacc
      841 ggcattgaca gggagcgcta ctggtatgat acctatatcc ttcccggttt tgttgattcc
      901 cacgttcacg ttgagagttc catgctcacg ccggccggtt tcgcccgcct tgccgtcgtt
      961 cacggaacgg tggcgaccgt ttccgacccc catgaaatag ccaatgtcct ggggccgcag
     1021 ggcgtgcgtt tcatgatgga ggacgccgcg ggaataccct tcgtttttgc tttcggcgtg
     1081 ccctcctgcg ttcccgcgag tccctttgag acgtcgggcg ccgcgatggg cctcgccgag
     1141 atggaggccc tctttgacga gcaaggcgct ggatatctca gcgagatgat gaacgtcccc
     1201 ggtgtcattg gcggcgaccc ggacgtgcag gcgaagatag aggcggcaag acgaagagga
     1261 aagccggtgg acgggcacgc accgggcctt cgaggagaag ggttgcgcag gtacatagca
     1321 gcgggcatat ccacggacca cgaatccttt gagcttgagg aggcgcgcga gaaggtctcc
     1381 ctgggtatga agatactggt ccgtgaaggt tcggcggcaa ggaacttcga tacgctctat
     1441 cccctcatcg acgaatctcc gggcttctgc atgctctgca ccgatgacca gcaccccgat
     1501 tccctcgtaa agggacacat caacacactt gtcgcccggg ccatcagaaa aggcgtggac
     1561 gttatgaagg tcctgaggtg tgcctgtgtc aaccctgtcc ttcactacgg tctcgacgtg
     1621 ggtctgctca gaccttctga ccctgccgac atgatcgttg tcgacggtct tgaggacctt
     1681 gaggtcttga agaccatagt acgcggcgag atcgttgccg aagccggtga aagccgcata
     1741 ccgcatcgca gggccacccc ggtcaacatt tttcgggcga ccgtcaggaa accggaggat
     1801 tttcgggtcc tcaagaggtc cgacgccatt cgtgtcatcg gggttataga aggtcaggtc
     1861 gtgacctatg ctcacctcga ggctcccgcc cttgaggggg acgcactggc ctgcgatacg
     1921 gacagggaca tactgaagat agctgttgtg aaccgctatg cagatcggcc gcccgcggtg
     1981 gccttcgtga ggggattcgg cctgaagaaa ggcgccatgg cctcgtccat tgcgcatgat
     2041 tcccacaata tcgtcgctgt gggcacgagc gatgagatgc tctgcgacgc cgtcaatctt
     2101 gtcatcgaga agaagggcgg catctcgctt gtctcggagg agatgaagat ggtgctgccc
     2161 cttcccgtgg cgggtctgat gtcgaacgag gacggttaca gtgtcgccga aaggtacagc
     2221 cgtctcacct tgcttgcccg ggaccttggc acgacgctca aggcgccctt tatgacgctt
     2281 tccttcatgg ccctcgtggt gatcccgaaa ctcaaactga gcgacaaggg actcttcgac
     2341 ggggagaagt tcactttcac ggacctcttt gtgtgaaagg gttcgacttg ccgttttaat
     2401 gggttcaata ggttacatgg gtcccatatg acccgggaaa agaagagctc cacttgtcat
     2461 gctactattt tgatgaattt ctttacgaga tcggggtcga actgagtgcc ggcgcacctg
     2521 cgcagttctt tgagcgcttc ctccctggac accgccttgc ggtggggccg cccgccggtc
     2581 attgactcgt aggcatctgc tatggccatg atcctgcttt cgaggggtat ctcctcaccc
     2641 gtgagaccca aggggtaccc gctgccatcc caccactcat gatgtttgag aatatagtcc
     2701 gctatgggtg tcagggttgc ggccgattgg gcgatgcggt gaccgatatc gcagtgcttg
     2761 cgcatctcgt tgagttcttc ctcggtgagg ctgcccgact tcaggagtat gcggtcgtgg
     2821 atgctgatat tcccgatatc gtggaattgc gcgagaagag cgaggtcctt caggcgcttc
     2881 tcggtcaggc ccttgaacag
//
LOCUS       DAOQTR010000286         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_40477_length_2894_cov_4.647411, whole genome shotgun sequence.
ACCESSION   DAOQTR010000286 DAOQTR010000000
VERSION     DAOQTR010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40477_length_2894_cov_4.647411"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PKN85_07420"
     CDS             <1..1235
                     /locus_tag="PKN85_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44277.1"
                     /translation="AVDAMKHGAYDYIPKPFNPDQLMAVINRGLDKRRLTIEKEKLRE
                     ERDQRLLEVASEKSKLHTIVNSMADGILVINREHQLVLWNPAVLKMLNVRGRVDESGV
                     GMDFQKALKQQDLIDVINKAFSPDSSQYTIISEEVVLAGETPKTLMVNVAGVRDERGE
                     NLGVVCTFRDISRLKEVEEIKSQFVRMVAHEIRAPLGAIEGYLNAYLTGVAGTDPQFL
                     RQMLERAKLRARSLMDLVNDLLLFAKLESKKVVRKKELLDITEIIENTVELFRVQGST
                     KDVSFFVEIPEKLPLIEADRSEMEQLFTNLVSNAMKYNVKNGKVTVRAVPEPHYLSIS
                     VSDTGIGMEEECLSCIFDEFYRVQGPNTRYTTGTGLGLSIVKRIVESHFGRIEVESAV
                     GKGTVFTVRLPLKQVNDK"
     gene            1274..>2894
                     /locus_tag="PKN85_07425"
     CDS             1274..>2894
                     /locus_tag="PKN85_07425"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNT44278.1"
                     /translation="MATKKASKTNQILEKKVLAPSITMYKLYVPDIARKVKAGQFVVV
                     RGDDMGERIPLTVADYDTKKGTITIIFQEVGTSTQKLAKFEAGMALLDVVGPLGKPSH
                     IEKFGTVVCVGGGVGVAPVLPIAKALKEAGNEVISIIGARTQSMLILEEEMKKASTTV
                     HVTTDDGTYGHHGFVTDVLKKIIEERGAANIALVVGIGPVIMMKAVTDVTRPFGVKTV
                     VSLNTIMVDGTGMCGCCRATIAGETKFVCVDGPEFDGHAVDFPELVLRQKMYNREERR
                     ALWDHKCKIDAQEKAVKKARKRVKMPEQAPKQRIQNFAEVALGYTKENAMMEAQRCLG
                     CKKPPCVEGCPVNVQIPQFIAKIKEGDFMSAIHIIKETNSLPAVCGRVCPQESQCESK
                     CILGKKSEPVAIGRLERFAADYQLGLGDVRVPTLPKKTGKKVAIIGAGPAGLTVAGEL
                     SKKGHECTIYEALHKAGGVLVYGIPEFRLPKAIVQREVDYLEKLGAKVKVDSIVGQTV
                     TLDELFAQGYDAAFVGTGAGLPYFLNIPGENL"
BASE COUNT          678 a          859 c          861 g          496 t
ORIGIN      
        1 cggctgtcga cgcaatgaaa cacggcgcct acgattacat acccaagcct ttcaacccgg
       61 accagctcat ggcggtcatc aaccggggcc tcgacaagag gcggctcacc atagaaaagg
      121 aaaagctccg cgaagagagg gaccagcggc tcctcgaagt ggcaagcgag aaatcaaagc
      181 tccacaccat cgtgaactcg atggccgacg gtatcctcgt catcaacagg gagcaccagc
      241 ttgtcctgtg gaacccggcg gtgctcaaaa tgctcaacgt gcggggcagg gtcgacgagt
      301 ccggagtggg catggatttc cagaaggcgc taaagcagca ggacttgata gatgttatca
      361 acaaggcgtt ctcccctgat tcttcccagt atacgatcat ctccgaagag gtggtcctcg
      421 caggggagac cccgaagacc ttaatggtca atgttgcggg agtgcgcgac gagagaggcg
      481 agaacctcgg cgtcgtctgc accttccgcg atatctcccg tctcaaggag gtcgaggaga
      541 taaaatccca gttcgtgagg atggtggcgc acgagatacg ggcgccgctg ggggcgatcg
      601 aagggtatct caacgcctat ctcacgggag tggcgggcac ggacccccag ttcctccggc
      661 agatgctgga gcgggcaaag ttgagggcaa ggtcgctcat ggacctcgtc aacgaccttc
      721 tgctcttcgc gaagctggaa tcgaagaagg tcgtaaggaa gaaagagctc ctggacatta
      781 cggagatcat cgagaacacc gtcgagctct tcagggttca gggttcgacg aaggatgtgt
      841 cgttcttcgt cgagataccc gaaaagctgc ccctcatcga ggccgacagg tcggaaatgg
      901 agcagctctt cacgaacctt gtgtccaatg ccatgaagta caatgtcaag aacggcaagg
      961 tcacggtcag ggccgtgccg gagccccatt acctcagcat aagcgtgagc gacacgggca
     1021 tcggcatgga ggaggagtgt ctctcctgca tcttcgacga gttctaccgg gtgcagggac
     1081 cgaacacgcg ctatacgaca ggcaccggat tggggctttc catcgtgaaa agaatagtgg
     1141 aatcccactt cgggcgcatc gaggtcgaaa gcgcggtcgg gaaaggaacg gtcttcaccg
     1201 tcaggctgcc gctcaagcag gtgaacgaca agtaattgtt ttgatagaat taaacttttg
     1261 aaaaggagac gttatggcta ccaagaaagc atccaaaacc aaccagatcc tggagaagaa
     1321 agttcttgct ccctcgatca ccatgtacaa gctgtatgtc ccggacatcg ccagaaaggt
     1381 gaaggccggc cagttcgtcg tcgtccgcgg cgacgacatg ggcgagcgca taccgctcac
     1441 cgttgccgat tatgacacca agaagggcac catcaccatc atattccagg aagtgggcac
     1501 atccacccag aagctcgcga agttcgaggc gggcatggcc ctcctcgacg tcgtcggccc
     1561 cctgggtaaa ccgagccaca tcgagaagtt cggcaccgtt gtctgcgtgg gcggcggcgt
     1621 cggcgtggcg cctgtcctgc ccatcgccaa ggccctcaag gaggcaggca acgaggtcat
     1681 ctccatcatc ggcgcccgca cgcagagcat gctcatcctc gaggaagaga tgaagaaggc
     1741 gagcaccacc gtgcatgtca ccaccgatga cggcacatac ggacaccacg gttttgtcac
     1801 cgacgtcctg aagaagataa tcgaagagcg cggcgctgcc aacatcgccc tcgtcgtggg
     1861 tatcggcccc gtcatcatga tgaaggccgt caccgacgtg acccgcccct tcggcgtcaa
     1921 gaccgtcgtc agcctcaata cgatcatggt cgacggcaca ggtatgtgcg gctgctgccg
     1981 cgccaccatc gccggcgaga cgaagttcgt ctgcgtcgac ggtcccgagt tcgacgggca
     2041 tgccgtggac ttccccgaac tggtcctgcg ccagaagatg tacaaccgcg aagagcggcg
     2101 cgccctctgg gaccacaagt gcaagatcga cgcacaggaa aaggccgtca agaaggcccg
     2161 caaacgcgtg aagatgccgg agcaggcacc gaagcagcgg atccagaatt ttgcggaagt
     2221 ggccctggga tacacgaaag agaacgccat gatggaagcc cagcgctgcc tcggctgcaa
     2281 gaagccgccc tgcgtggaag gctgccccgt caacgtacag atcccccagt tcatcgcgaa
     2341 gatcaaggaa ggcgacttca tgagcgccat ccatatcatt aaagaaacga acagccttcc
     2401 cgccgtctgc ggcagggtct gtccccagga aagccagtgt gagtcgaaat gtatcctggg
     2461 caagaagtct gaacctgttg ccatcggccg cctcgagcgc tttgccgcgg actatcagct
     2521 cggtctcggc gatgtccgcg ttcccacgct gccgaagaag acgggcaaga aggtcgccat
     2581 catcggtgcc ggccccgccg gtctcaccgt cgcaggcgag ctgagcaaga agggccacga
     2641 atgcaccatc tacgaagcgc ttcacaaggc gggcggcgtg ctcgtttacg gcatccccga
     2701 gttccgtctc cccaaggcga tcgtgcagcg cgaggtcgac tacctcgaga aactcggcgc
     2761 caaggtgaag gtcgactcca tcgtcggcca gacggtcacc cttgacgagc ttttcgccca
     2821 gggttacgac gccgcgttcg tcggtaccgg cgcgggtctc ccctacttcc tcaacatccc
     2881 cggcgagaac ctga
//
LOCUS       DAOQTR010000287         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41023_length_2868_cov_2.820832, whole genome shotgun sequence.
ACCESSION   DAOQTR010000287 DAOQTR010000000
VERSION     DAOQTR010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41023_length_2868_cov_2.820832"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PKN85_07430"
     CDS             complement(<1..904)
                     /locus_tag="PKN85_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNT44279.1"
                     /translation="MWKVIYNLLTLCALPVFVVIGLANAKMRGNFRARLLPRVKGRPD
                     TGACMIHGASIGEAIIAEGVADYLAKDGGPAHFLFTANTSYAQEMLERKEGARDGRKV
                     ASLPFDLRFSVRRFLSHHNPSAIIIVETEIWPNLIWEAGRRRIPVVIVNGRISDSTLA
                     SYRRLSVFMRSALAGVDAVIAQSAEHRQRFISIGMAPERVFATGNIKYYRPVADSIPG
                     REERDRIIAIGSVKEKELDEIYRAVKLLKSDLPDHRIFIAPRELHLAATIEEELSKTF
                     RTARYSRIKEGTDEDRADIVVVDTV"
     gene            complement(996..1553)
                     /locus_tag="PKN85_07435"
     CDS             complement(996..1553)
                     /locus_tag="PKN85_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HNT44280.1"
                     /translation="MKKKIKSFLMLHVMPPMVFAFLTALRLTLRVEHVNRPPDEAWGN
                     VRNYIVCFWHGRLLMMPFAKKGGGGKVLISRHRDGEFIARIVNYFGIGTVRGSYRKGS
                     VSSLREILHDLKDNIDIAITPDGPKGPRFTVKKGIVELARLSGKALLPVSYGARKKKR
                     FIPGTASYSPTLSPGSFFSGETLSL"
     gene            complement(1553..>1957)
                     /locus_tag="PKN85_07440"
     CDS             complement(1553..>1957)
                     /locus_tag="PKN85_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT44281.1"
                     /translation="FILKLPKGYDTVVGEKGVRLSGGQKQRIAIARALYKNAPILILD
                     EATSSLDAQSEIEVQKALENLMKGRTTIIIAHRLSTIINAHRIIVIDRGMVVQEGTHG
                     SLIEQEGPYRKLYEIQFREGARKKVIPLGKKR"
     assembly_gap    1958..1967
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1968..>2868)
                     /locus_tag="PKN85_07445"
     CDS             complement(<1968..>2868)
                     /locus_tag="PKN85_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44282.1"
                     /translation="LIAFLILPFSIYPIVAFGKKLRKIGLNIQQYLARLTSFLHETIT
                     GQRIVKAFSMEGYENKRFTAENESLFRISMKRTKIRALSHPVMEVIGGVAVGVVIWYG
                     GREVILGNSTPGTFFAFMASLIMLYEPIKKINKENHNVQQGLAASQRVFEIIDRQPEV
                     MEKPDAAQLEKVEGVIDFENVSFRYDEKMVLNNINLRIEKNEVIAVVGKSGVGKTTLV
                     NLIPRFYDVTEGLVRVDGADIRDVTLDSLRRNIALVTQDVILFNDTIRSNIAYGVEPD
                     EERLFEATRMAFARVPAVEHAVTA"
BASE COUNT          512 a          818 c          885 g          643 t
ORIGIN      
        1 ccacggtgtc gacgaccacg atgtcggcac ggtcctcatc cgtgccctcc ttgatgcggg
       61 aatagcgggc ggtcctgaag gtcttcgaaa gctcctcctc gatcgtggcg gccagatgga
      121 gttccctggg cgctatgaat atcctgtgat ccgggaggtc ggatttgagg agcttgaccg
      181 ccctgtatat ctcgtcgagc tccttttcct tcacgctgcc gatggctatg atcctgtcgc
      241 gttcttccct gcccgggatg ctgtcggcaa cgggacggta gtacttgatg ttgcccgtgg
      301 cgaagacccg ctccggggcc atgccgatgg aaatgaagcg ctgcctgtgc tccgcggact
      361 gcgcgatgac ggcgtctacc ccagccagag ccgatctcat gaacacggac agcctgcggt
      421 aactggcgag ggtgctgtcc gatatgcgcc cgttgacgat aacgaccggg atgcgccgtc
      481 tgccagcctc ccagatcagg ttgggccaga tctccgtttc cacgatgatg atggccgagg
      541 gattgtggtg ggagaggaag cgtcgcacgg agaagcgcag gtcgaagggt agagaggcca
      601 ccttcctgcc gtccctggcg ccttcctttc tttcgagcat ttcctgcgcg tatgatgtgt
      661 tggcggtgaa gagaaaatga gccggcccac cgtccttcgc gaggtagtcg gccacgccct
      721 cggcgatgat ggcctctcct atcgacgccc cgtggatcat gcacgcaccc gtatccggcc
      781 tgcctttcac ccgcgggaga aggcgcgcgc ggaagttccc cctcatcttc gcgttggcaa
      841 gtccgatgac gacgaagacg ggcagggcgc agagggtgag cagattgtag atcactttcc
      901 acatgccgct tcttccgctc tttcggtcag ttggacaagc gttgcctcga gcttgaggcg
      961 cgttttctct gtttcttcgg cactgaggtc acggtctaca gggatagggt ctccccagag
     1021 aaaaacgacc cgggagaaag ggtaggggag tatgaagcgg tcccaggaat gaaacgtttt
     1081 tttttacgcg caccgtaact gacggggagg agggccttgc cggagagccg cgcaagctcc
     1141 acgatgcctt tcttcacggt aaagcggggc cctttcgggc cgtcaggggt gatggcgata
     1201 tcgatattgt ccttgaggtc gtggaggatc tccctgagcg aggagacgct gccctttctg
     1261 tatgagcccc ggacggtgcc gatcccgaag tagttcacta tcctggcaat gaactctccg
     1321 tcgcggtggc ggctgatcag caccttgccc ccgccgccct tcttcgcgaa gggcatcatg
     1381 aggagcctgc catgccagaa acagacgatg tagttgcgga cgttccccca tgcctcgtcg
     1441 ggcggcctgt tcacgtgttc aacgcgcagc gtcaacctca gtgccgtcag gaacgcgaat
     1501 accatgggag gcatgacgtg cagcatcagg aaggatttga tcttcttctt catcagcgtt
     1561 tctttcccag ggggatgacc ttcttgcggg ctccctcgcg gaactggatc tcgtagagct
     1621 tcctgtaggg cccttcctgc tcgatgagcg acccatgggt gccttcctgg accaccatcc
     1681 ccctgtcgat gacgatgatc cgatgggcgt tgatgatcgt ggagagcctg tgggcgatga
     1741 ttatcgtcgt cctccctttc atcaggttct cgagggcctt ctggacctct atctctgatt
     1801 gggcgtcaag ggaactcgtg gcctcatcga ggatgaggat gggggcgttc ttgtaaaggg
     1861 ccctggctat ggcgatgcgc tgcttctggc cgccggaaag acgcacccct ttttcgccga
     1921 cgacggtgtc gtatcccttg gggagcttga ggatgaannn nnnnnnnggc ggtcacggcg
     1981 tgctccaccg ccggcacccg ggcgaaggcc atccgggtcg cctcgaaaag cctctcttcg
     2041 tccggctcga ccccgtaggc gatattgctc cgtatcgtat cgttgaagag gatgacgtcc
     2101 tgcgtgacaa gggcgatgtt ccttctgagg gaatcaaggg tcacgtcgcg gatatcggcg
     2161 ccgtcgaccc tgacgagacc ctcggtgacg tcgtagaacc tcgggatcag attgacgagg
     2221 gtggttttcc ccacaccgct tttcccgacg acggcgatca cctcgttctt ctcgatccgg
     2281 aggtttatgt tgttgaggac catcttttca tcgtatcgga aggagacgtt ctcgaagtct
     2341 atgacaccct cgaccttctc gagttgggcg gcatccggct tctccatgac ctcgggctgc
     2401 ctgtcgatga tctcgaagac gcgctgactg gcggcgagcc cctgctggac gttgtggttc
     2461 tccttgttga tcttcttgat gggctcgtag agcatgataa gggatgccat gaaggcgaag
     2521 aaggtaccgg gggtggagtt tcccaggatg acctcccggc ccccatacca gatgacgaca
     2581 ccgacagcga ccccgccgat gacctccatc acggggtgcg atagtgcccg gatcttcgtg
     2641 cgcttcatgg agatcctgaa gagcgattcg ttctccgcgg tgaatctctt gttctcgtat
     2701 ccctccatgg agaaggcctt gacgatcctc tggccggtga tggtctcatg gaggaagctg
     2761 gtgaggcgcg ccaggtattg ctggatattg agccctatct tcctgagctt cttgccgaag
     2821 gcgacaatgg gatagatgga aaagggcagg atcaggaagg cgataagc
//
LOCUS       DAOQTR010000288         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41054_length_2866_cov_3.406973, whole genome shotgun sequence.
ACCESSION   DAOQTR010000288 DAOQTR010000000
VERSION     DAOQTR010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41054_length_2866_cov_3.406973"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1260
                     /locus_tag="PKN85_07450"
     CDS             157..1260
                     /locus_tag="PKN85_07450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT44283.1"
                     /translation="MKIFVTGTRGIPDIPGGVEKHCQELFPRIARSGHEVILATRASY
                     VTDRLDRWKGVKLVHLFAPRKKSLEAIIHTTLAVLTARLHNPDILHINAIGPSLLVPL
                     ARLLGMKVVMTNHGPDYDRQKWGRLAKAMLRLGEKLGGLFANEVIVISNVIADILRTR
                     CGRESHLIYNGVPIPEPSTSTDYLEGIGAETGRYVLAVVRFVPEKGITDLIQAFTGMD
                     TEMKLVIAGDADHETEYSRKIRQLARADSRIILTGYITGEPLHQVFSHARLFVLPSYH
                     EGLPIALLEAMSYGLDVLVSDIPANKEVGLAEDRYFICGNVEDLRCRMEHRLSRRMAE
                     KDREAMRREVQQRYNWDDIAKQTIAVYERARKG"
     gene            1265..2665
                     /locus_tag="PKN85_07455"
     CDS             1265..2665
                     /locus_tag="PKN85_07455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNT44284.1"
                     /translation="MYQDQVRILNTFRMIADAVCIIAAAYAALLTPKVLFPWQVTIST
                     MNFVTTIAIVVTANNYIMGRFRLYSDRKARSILSIVWSITRTIVADFAIISMWVLLYD
                     PLSASRGFFILFLFYSYLFLVGERVIVQLYFDRIKLKRSDLRKVLIVGDLERGSIVAQ
                     QMQRQLSWGHQIVGHFTIGKRSEKARSLNTPEELVDILHRANIDEVIFTLEADPSIDL
                     VSYLDKCRVMGVTSRVVPAMWRPGARHMSTEYLQGVPFLTFQTNNFNATGMLYKRILD
                     IVGGMVGTFLFLAMYPFVALAITCDSPGPVMFRQKRVGRNGRIFEVFKFRSMYADAEK
                     RKKELMERNEMEGAIFKIKDDPRITRVGRWLRKTSIDEVPQFLNVLKGEMSLVGTRPP
                     TVDEVEKYQDWQFKRISAKPGITGLWQISGRNEITDFDSIVDLDTQYLENWRFLDDVR
                     ILLMTIWVVIRGKGAE"
BASE COUNT          669 a          772 c          838 g          587 t
ORIGIN      
        1 acaatattgc tcgttttatc atttccgtgt aactaccact gtcgaacccg ggatagagag
       61 attaatgcaa aaggcaaaca taaataacta taatatagca actgcaaggt tgggtccgtt
      121 ccctatgcat cactacgttt atggtaaggg ttgattgtga agatcttcgt caccggcaca
      181 cggggcatcc cggacattcc cggcggggtc gagaagcact gtcaggagct gttccccagg
      241 atcgcccgga gcgggcacga ggtcattctg gccaccaggg catcgtacgt gacggatcgt
      301 ctggatcggt ggaagggggt taaactcgta cacttgttcg caccacggaa gaagagcctg
      361 gaggccatca tccacaccac gctggccgtg ctcactgccc gcctgcacaa ccccgacatc
      421 ctccacatca acgccatcgg gccttctctg ctggtccccc tcgccaggct tctcggcatg
      481 aaggtggtca tgacgaacca cggccccgac tacgataggc agaagtgggg caggctcgcc
      541 aaggccatgc tgcggctggg agagaaattg ggaggcctgt tcgcaaacga ggtcatcgtc
      601 atctcgaacg tcatagcaga catcctcagg acacggtgcg gaagggaatc acacctcatt
      661 tacaatggcg taccaattcc agaaccgagc acttcgacag actatttgga aggcatcggt
      721 gcggagaccg gcaggtatgt cctggccgtc gtacggttcg tcccggaaaa gggcataaca
      781 gacctcatcc aggcattcac gggcatggac acggagatga agcttgtcat cgcgggcgat
      841 gcggaccatg aaaccgagta cagcaggaag atcaggcagc tcgcccgggc cgacagccgc
      901 atcattctga ccgggtacat caccggcgaa ccgctccatc aggttttctc ccacgcgcgc
      961 ctctttgtgc tcccatcgta tcacgaaggc ctccccatcg ccttgctcga ggccatgagc
     1021 tacggcctcg acgtactggt ttctgacatt cccgcgaata aggaagtggg cctcgccgag
     1081 gacagatatt tcatctgcgg gaatgtggag gacctcagat gcaggatgga acaccggctt
     1141 tccagacgga tggctgaaaa ggacagggag gccatgcgtc gcgaggttca gcagaggtac
     1201 aactgggacg acatagcgaa gcagaccata gcggtctacg aaagagccag aaaaggatga
     1261 cgacgtgtac caagaccagg tgaggatcct caacacattc cgcatgatag ctgacgcggt
     1321 gtgcatcatc gctgcggcct atgcggcact gctgacaccc aaggtactgt tcccctggca
     1381 ggtgaccata tccaccatga acttcgtcac cactatcgcc atcgtcgtga cggcgaacaa
     1441 ctacatcatg ggtcggttca gactctacag tgaccgcaag gcacgttcga tactcagcat
     1501 cgtgtggtcc ataaccagga ccatcgttgc cgacttcgcc atcatctcca tgtgggttct
     1561 cctgtacgat ccactctctg catcgcgggg tttcttcatc ctgttcctct tctacagtta
     1621 tctctttctc gttggtgagc gcgtgatcgt gcagctctat ttcgaccgga tcaagctgaa
     1681 gcgatccgac ctccgcaagg tcctcatagt cggggacctc gaacggggta gcatcgttgc
     1741 acaacagatg cagcgccagc tgagctgggg ccaccagatc gtcgggcact tcaccatcgg
     1801 gaaacggtcc gagaaggcca ggagcctgaa cacgccagag gaactcgtgg acattctcca
     1861 cagggcgaac atagacgagg tcatctttac cctggaagcg gacccttcca tagacctggt
     1921 atcttatctg gataaatgca gggtcatggg ggtgacgtcc cgggtggtgc cggccatgtg
     1981 gaggccgggg gccagacaca tgagcaccga gtatcttcag ggtgttccct tcctgacctt
     2041 tcagacaaac aacttcaacg ctacgggcat gctttataag aggatactcg acatagttgg
     2101 tggcatggtg gggacgttcc tcttcctggc gatgtatccc tttgtggccc tggccatcac
     2161 gtgtgattca ccaggcccgg tcatgttcag gcagaagagg gtcggcagga atggccggat
     2221 ctttgaggta ttcaagttca gatccatgta cgcagatgca gagaagcgca aaaaggagct
     2281 catggagagg aacgagatgg agggcgcgat cttcaagata aaggatgacc cacgcatcac
     2341 cagggtcggc aggtggctca ggaagacgtc tattgatgag gttccgcagt ttctcaacgt
     2401 gctcaagggc gagatgagcc tggtggggac gaggccgcct accgtcgacg aggtggaaaa
     2461 gtaccaggac tggcagttca agcgcatatc tgctaaacct ggcataaccg gcctgtggca
     2521 gatctcaggg cgcaacgaga tcacggactt cgacagtatc gtggatctcg atacccagta
     2581 cctggagaac tggcggttct tggatgatgt caggatcctg ctcatgacga tatgggtggt
     2641 gatccgaggc aagggagcgg aataggttac ctggacgagg ccgctttcga gaagacaacg
     2701 catgaaaagg ggtacgttat gcgggaaagc attgtgttca tcgttcttgc gatggtgttc
     2761 atgggtactt gcctgcgggc tgaggcggca tcggaagggt atgtgatcgg tgcgggagac
     2821 gttctcatca tctcccactg gaaggacgag gcactgacca gagagg
//
LOCUS       DAOQTR010000289         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41076_length_2865_cov_3.689680, whole genome shotgun sequence.
ACCESSION   DAOQTR010000289 DAOQTR010000000
VERSION     DAOQTR010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41076_length_2865_cov_3.689680"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKN85_07460"
     CDS             complement(<1..194)
                     /locus_tag="PKN85_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44285.1"
                     /translation="MKRAILLTVLVAIATVFLLSLGAGTVKAQAGSGGAQDQPAAPAW
                     RAQMEAADLMISEAEKTKKR"
     gene            complement(586..1236)
                     /locus_tag="PKN85_07465"
     CDS             complement(586..1236)
                     /locus_tag="PKN85_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT44286.1"
                     /translation="MTDPRDSAVYLLDLGEPVLIDCGSGHGLERMVENVRKAGFSPAD
                     IKALIMTHCHVDHIGAAPLLRSRFGTRLVIHEKDAEILERGDIRLTAAFCFDVELKPL
                     AVDRKLVGETGTLRFGNHDLTWVHTPGHTPGSISLYMDVDGERILFAQDIGAPLLAEF
                     DCDPAAWLESIQKLFALNADVLCDGHSGAYGPKDVVRQYLEYCLASQYRQGYITAG"
     gene            complement(1356..>2865)
                     /locus_tag="PKN85_07470"
     CDS             complement(1356..>2865)
                     /locus_tag="PKN85_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44287.1"
                     /translation="RWSLKDMRLADIAASREHVKTVVDDNLGELTEEILSASTLYRLI
                     TEHRPEIIVDCVNTATALSYQNIHHAYEAVDTDAALVDGMSAIYEVLSATSIPPLIRH
                     VQILYQAMKEAGTRLYLKIGTTGTGGMGLNIPFTHGEESPSRLLMAKAAAAGAHTMLL
                     YTLSKTPGWPIIKEMKPAAMIGWKDIGKGPVRRGNKGYPLYDCPVESAYLLKEGSTFS
                     YEGSDAGSPLGGRQLEGVYIDTGENGVFSIDEFKAITSLGLMEFVTPEDIAHNVIMNI
                     IGVNSSKDVIGAIDNAVMEPTFRAGILRRDAIEEAVRLGSGGIAYGFLGPRMSKLVTE
                     CKLIKQSYGTVEDALSVSPERLAYSLEKLLEVEPEMRSAPISIGIPILLPDGERLIFA
                     RRAVKDKSWEERPWAVTTANIDRWAEREWVDLRPANMARWQERFRKASEDACRFPEKR
                     CSRFNRGMLFWKKTEEGKTIIDPGEITAWVFINEFAGGRPHAYSESKEMVYF"
BASE COUNT          537 a          859 c          820 g          649 t
ORIGIN      
        1 caccgcttct tggtcttctc cgcctcggat atcatcagat ccgcggcttc catctgtgcc
       61 ctccaggccg gggcggccgg ctggtcctgg gcccctccgc tgcctgcctg ggccttaacc
      121 gtgccggctc ccagggaaag aaggaaaacc gttgctatcg cgaccaacac tgtcaacaaa
      181 attgctcttt tcatagttcc ctctccttgt ttgtaaagaa tgtttgtagc ctgtcattca
      241 tccttttcga tgaacctggt agtgaccgcc tgcacctcct tctggtcttt cgatccccgg
      301 cacatttccc cttttcggtg ccatactcac tggagcaatc ggtcctaaca ggtacgatcc
      361 aacgctgcat ctgaacgtgc tcgatgctca tgattatagg ataatcaagg caatccgcct
      421 tgtctgtcgc agaaagtatg attatcgcat agccattttg atgatttccc tgtcatcctt
      481 ttcgatgaca ggcgccggga tgtgcagcgg gcggttgaac ggggttgtcc atgaaggagc
      541 cgggcagaca atgcgaccat atgcggggct tgaggcccgc gggtatcacc cggcggtgat
      601 atagccctgc ctgtattgcg aagcgaggca gtattcgagg tattgtctga caacgtcctt
      661 cgggccatag gctccggaat ggccgtcgca gaggacatcg gcgttaaggg cgaagagttt
      721 ctgtatggac tcgagccagg ccgcagggtc gcagtcgaat tcggcgagca ggggtgcccc
      781 tatgtcctgg gcgaagagga tgcgctcacc gtccacatcc atgtaaaggg agatcgaccc
      841 gggcgtgtga cccggcgtgt ggacccacgt gaggtcatgg tttccgaagc ggagcgtccc
      901 cgtttccccg acaagctttc tatcgacggc aaggggtttg agttcgacat cgaagcaaaa
      961 ggctgcggtg agccgtatat ccccgcgctc gaggatctcc gcatcctttt cgtggatgac
     1021 cagccttgta ccgaaacggg agcgcaggag aggcgcggca ccgatatggt cgacatgaca
     1081 gtgggtcatg atgagagcct tgatgtcggc ggggctgaaa cctgccttcc gaacattttc
     1141 gaccatgcgc tcgaggccgt gccccgaccc gcagtcaata agcaccggct cgccaagatc
     1201 gagaagatag acggcgctgt cccttggatc cgtcatatcc gccccggcaa cgcgccacat
     1261 aatgtcggtt atccgttgag gtcccacgac gaacacacac ccggtttctt tccgttttca
     1321 cctggaacct ggaacctgga acctggaacc ttcatttaaa aatacaccat ctccttcgac
     1381 tcgctgtagg catgcggcct gccgcccgcg aactcgttga tgaagaccca cgccgttatc
     1441 tcgccggggt cgatgatcgt cttgccttcc tccgtcttct tccagaagag cattccgcgg
     1501 ttgaagcggg agcagcgctt ctcggggaac ctgcaggcgt cctcgcttgc cttccggaac
     1561 ctctcctgcc atcgtgccat attggccggt ctcaaatcca cccattcccg ctcggcccat
     1621 ctatcgatgt tggcggtggt gacggcccag ggtcgttcct cccagctctt gtccttcacg
     1681 gcccgtctgg cgaagatgag gcgctccccg tcgggcagga ggatgggtat cccgatggaa
     1741 atgggggcgc tgcgcatctc gggctcgacc tcgagaagct tctccagcga atacgccagg
     1801 cgctcgggcg agacggaaag ggcgtcctcg actgtgccgt agctttgctt tataagcttg
     1861 cattcggtga cgagcttgga catccttggc cccaggaagc cgtaggcgat accgccgctt
     1921 ccgagacgga cggcctcctc gatggcgtcc ctgcgcaaaa tgcccgccct gaaggtgggc
     1981 tccataacag cgttatcgat cgctccgatg acatccttcg aagagttgac gcctatgatg
     2041 ttcatgatca cgttgtgggc gatgtcctcg ggggtcacga attccatgag gccgagagac
     2101 gtgatcgcct tgaactcgtc gatggagaag acgccattct cgccggtatc tatgtacacc
     2161 ccctcgagct gtcttcctcc caggggggaa ccggcatcgg atccttcata agagaaggtc
     2221 gacccctctt tgaggaggta cgcgctttcg acggggcagt catagagagg gtagcccttg
     2281 ttcccccgcc gtacgggccc ttttccaatg tccttccatc cgatcatggc cgcgggtttc
     2341 atctccttga tgatgggcca ccccggcgtc ttgctcaggg tatagaggag cattgtgtgc
     2401 gccccggcgg cggccgcctt tgccatgagg agcctcgacg gactctcttc gccatgcgtg
     2461 aaaggaatgt tgagacccat gccgcctgta cccgtggttc ctatcttcag atacagccgg
     2521 gtccccgcct ctttcatcgc ctggtagagt atctgaacat gccggatgag cggagggata
     2581 ctcgtcgccg acagcacctc atagatggcg ctcatgccgt ccacgagggc tgcgtccgtg
     2641 tccaccgcct cgtatgcgtg atgaatgttc tgataggaca aagccgtggc ggtgttgacg
     2701 caatcgacga tgatctccgg cctgtgttcg gtgatgagtc tgtaaagggt tgacgcgctg
     2761 aggatctcct ccgtgagctc gccaagattg tcatcgacca ccgtcttcac atgttccctg
     2821 gaggcggcga tatcggccag acgcatatcc ttcagggacc acctg
//
LOCUS       DAOQTR010000290         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41086_length_2865_cov_3.011388, whole genome shotgun sequence.
ACCESSION   DAOQTR010000290 DAOQTR010000000
VERSION     DAOQTR010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41086_length_2865_cov_3.011388"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKN85_07475"
     CDS             complement(<1..645)
                     /locus_tag="PKN85_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNT44288.1"
                     /translation="MAGKQDYYELLGVSKTASEDDIKKAYRKLALKYHPDRNPGNKEA
                     EEKFKLINEAYAVLSDPEKRKQFDAFGMGGFQQRYSEEDIFRGFNVGDLFKDLGFGGG
                     DIFSMIFGRQAGRGARQQRQQSWDFNDYITRQQQGTQAARDLDLHYELEIPFMDAIHG
                     AEKRISLATATGTEEVNVKIPKGIASGKKLRLKGKGNQGRSGQRGDLYITMKVAE"
     gene            complement(654..1427)
                     /locus_tag="PKN85_07480"
     CDS             complement(654..1427)
                     /locus_tag="PKN85_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNT44289.1"
                     /translation="MVRKTTALSGNTPEVLDSSLSLLEELFVGDATPPEIAGKLLSHI
                     RRRTGVWDPYATLKYREYRDALAAVGGLRGGECLEDLLSLSALGNSTDYFTGGTFMNE
                     LPVIEGDVDLAAAAISRESDEMLLLGDNMGDFVFDLPLVRFLERNGRKVFYAVREHPA
                     QNDLSMEDVRRLGLHDLHPRIISTGKANVGLPREDIAGEIERLWKGESPVIAKGMGNF
                     ETISEFDGERQVVYIMKVKCPAVARAVGNDIGTYIAHVR"
     gene            1717..2658
                     /locus_tag="PKN85_07485"
     CDS             1717..2658
                     /locus_tag="PKN85_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44290.1"
                     /translation="MADKRRTSVYVDYHILDLISMTRVSDEALLSEWKAGRSIWDRRG
                     QEMVSLVTSVDEMELDFVIQMNRGGLCVTDTFQITDNIDNFERWEGADHADTDHWRAI
                     VELYDQLDVISGHDDLVGEHAYPHYCEQLARVLKEEPAGDTGRSASFDEETAILRDCA
                     AALHDVYDMQLWADLKHIQYGLNWKVLRSVLPRYSRSAVLNGEDAGLNKNLLGLLNRL
                     VNIGKKSCPRLPMQDRHIDFVLDIVRKKYCQEDIDRSISHIAHCLRGGIDCYLTTDGE
                     LAERFAERKQNLQLALGTSIHLELLRPTELEKKLGAA"
BASE COUNT          578 a          865 c          791 g          631 t
ORIGIN      
        1 ttccgccacc ttcatggtta tataaaggtc accacgctgc cctgaacgcc cctggttgcc
       61 cttgcccttc agcctcagct tctttccgct cgcgatccct ttcggtatct tgacgttcac
      121 ctcttccgtt cccgtcgccg tggccaggga tatccgcttt tccgcgccgt gtatggcgtc
      181 catgaaaggt atctccagtt cgtagtggag gtcgaggtcg cgcgctgcct gcgtcccctg
      241 ctgctgcctc gtgatgtagt cgttgaagtc ccacgactgc tgcctctgct gccttgcccc
      301 gcgacccgcc tgccttccga agatcatgct gaagatgtcc cctccgccga agccgaggtc
      361 cttgaagagg tctcccacgt tgaagcccct gaagatgtcc tcctcgctgt agcgctgctg
      421 gaagcctccc ataccgaagg catcgaactg cttgcgtttt tcggggtcgc tcagcaccgc
      481 gtaagcctcg tttatgagct tgaacttctc ctcggcctcc ttgttgcccg ggtttcggtc
      541 gggatggtac ttgagcgcca atttcctgta cgccttcttt atgtcatcct ctgaggccgt
      601 cttggagacg ccgagcaact cgtagtaatc ctgtttccct gccatacggt ctgtcacctc
      661 acgtgagcta tgtacgtccc tatgtcgttg ccgactgccc gggcaacggc ggggcatttc
      721 accttcatta tatataccac ctgacgttcg ccgtcaaatt cgctgatggt ctcgaaattc
      781 cccatgccct tcgcgatgac cggactttcc cctttccaga gcctttcgat ctcccccgca
      841 atgtcttccc tcggcagacc cacgttggct ttcccggtcg atatgatgcg cgggtgcaga
      901 tcgtgaagtc caaggcgccg gacatcctcc atggaaaggt cgttctgcgc gggatgctcc
      961 ctgaccgcat agaacacctt cctgccgttc ctttcgagaa agcgcacgag gggaagatcg
     1021 aagacgaagt cgcccatgtt atcgccgagc aggagcattt catcgctttc gcgagagatc
     1081 gccgccgccg ccagatccac atcgccctcg atgacgggga gctcattcat gaatgtcccg
     1141 ccggtgaagt agtccgtgga gttgccgagg gcggacagac tgagcaggtc ttccaaacac
     1201 tcgccgccac gaagtccccc gacggcggcc agagcgtctc tgtactcgcg atatttgagg
     1261 gtggcgtagg gatcccagac gccggtcctt cgcctgatgt gcgacagcag ctttccggct
     1321 atctccggcg gggtcgcgtc cccgacgaaa agctcctcga gaagcgacag tgacgaatcg
     1381 aggacctccg gggtattccc ggaaagggcc gtcgtcttcc ggaccaatcc ttccagacat
     1441 gcgcgacagg gctcctgaac tttcatgaca ggcaatagga tataatcaaa tgatatgcat
     1501 ttcaacgtca aaggagcgcc gtggcgctcc cgaaataaac gctttcattc ttttcctcat
     1561 ttgttataat gggggcaggg gcttaagggt ggttccaggc catacggatt gacgctcatc
     1621 ctcacgttcg tcgctcaaat tttctttctc gtcgcctgac atgctcttcc ggcacgatct
     1681 atctttcacg gggattcccc ggacaaaggg tgacagatgg cagacaagcg gcgcacgagc
     1741 gtctacgtgg attatcacat tctcgacctc atctcgatga cccgggtgag cgatgaggcc
     1801 ctcctatccg agtggaaagc gggcagatcg atctgggacc gtcgcgggca ggagatggta
     1861 tctcttgtca cctccgtgga tgagatggag ctcgatttcg ttatccagat gaaccgcggc
     1921 ggcctctgcg tcaccgatac ctttcagatc accgacaata tcgacaactt cgaacgctgg
     1981 gaaggcgcgg accacgcaga taccgatcac tggagggcca tcgtggaact ctatgaccag
     2041 ctcgacgtca taagcgggca tgacgatctt gtcggggaac atgcctatcc ccactattgc
     2101 gagcagctgg cccgcgtcct gaaggaggag cctgccggag acacggggag gtcggcctcg
     2161 ttcgatgagg agacggcgat actcagggac tgcgcggcgg cgctgcatga cgtctatgac
     2221 atgcagctct gggcggacct caagcacatc caatacggcc tcaactggaa ggtcctccga
     2281 tccgtcctgc cgagatattc gcgcagcgcc gtgctcaacg gcgaggacgc ggggctgaac
     2341 aagaaccttc ttgggcttct caaccggctc gtcaacattg ggaagaagag ttgcccccgg
     2401 ctgcccatgc aggaccggca catcgacttc gttctcgata tcgtgaggaa gaagtactgc
     2461 caggaggata tcgaccgcag catctcgcac atagcccact gcctgcgggg cggtatcgac
     2521 tgctacctta ctacagacgg tgaactggcg gagagattcg ccgaaaggaa acagaacctc
     2581 caactcgccc tgggaacatc catccacctg gaactcctgc gcccgacgga actggagaaa
     2641 aagctagggg cggcttgaat tgcttgagta gcttgagtcg cttgagagtt aaatgaaaag
     2701 gaagacggag agcgatttgg agcaggcagc aacggtgggg ataaagagac cgtgaacccc
     2761 ggcctttgcc gggacaaaca accttgcggt tcgatcaggt tcttcaaccc tcaacagcct
     2821 gttgataaac tctgtctcac ggcatgatcc cgctcggtgg agaaa
//
LOCUS       DAOQTR010000291         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41221_length_2859_cov_3.635877, whole genome shotgun sequence.
ACCESSION   DAOQTR010000291 DAOQTR010000000
VERSION     DAOQTR010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41221_length_2859_cov_3.635877"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2472)
                     /gene="polA"
                     /locus_tag="PKN85_07490"
     CDS             complement(<1..2472)
                     /gene="polA"
                     /locus_tag="PKN85_07490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNT44291.1"
                     /translation="MTENTSAATYKRVFLVDGHSYLYRAFYATPHLSNSRGVPTNAIY
                     AFVSMIRKLASSENPDTLIVVFDSRGPSFREEISREYKAQRPAMPDNLSLQVPFVKQI
                     IDAMGLPVVEKEGFEADDIIATITEHLRTRDDVMTFIVTGDKDMMQLVGDRVRILDSM
                     KNVVIGEAEVIEKFGVPPRNVVDYLSLCGDTSDNIPGVPGIGEKTARELVSSLGSVEE
                     IYARLDEIKRKAVRERLVAGREKAEMSKKLATLRYDVPIETGVEGLIERPPDLETLRR
                     MYRELEFTALYREVRVQGTQKKDVREKALSELATDRVSIAASFRGKNAGDLALEAFAA
                     FDGEGVFFSADGADLTAVLRSAKEVIGHDLKPLFVVAGDLLAEKKVFDTMLAAYLANP
                     LRKDFAPGPIAEEYLDADISAMDASSSLRDLAACLPDLADALRARLGELDLSDLFFGT
                     ELPLVAVLADMEKAGVRIDRRRLGELSRDIDQRLTGIVKDIYAHCGGPFNINSPQQLS
                     RVLFEILGLTPVRKTKTGFSTDTAVLETLSESHPLPGRILEYRTLTKLKNTYIDVLPT
                     LINPLTGRIHTTFNQMVAATGRLSSSDPNLQNIPVRGQEGPKIREAFVPADGCLILSS
                     DYSQIELRVLAHLSGDRELIATFHRDEDIHSTVARDVFHVTADAMTQDMRRAAKVINF
                     GIIYGMSAFGLARELGVGQKEAQEYIDSYFERHRAVREFMDSVIEEARVKGFVRTLLG
                     RMRFIPELGNSDPAVRQLGERVAMNTPIQGTAADIIKMAMINIRRATRLKGLSSRLIL
                     QIHDELLFEVAPDETTVTTQLVAQGI"
     gene            complement(2492..>2859)
                     /locus_tag="PKN85_07495"
     CDS             complement(2492..>2859)
                     /locus_tag="PKN85_07495"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44292.1"
                     /translation="IGLTEEETRNRVKDLKDGGRIRRIGPVLDAKRLGYTSLLCGVAI
                     PPDRLGQISGTVSEEPGVTHNYEREGELNLWFTVTMERKEDIERFLSMLEETFSITIQ
                     RFPEKRTFKIRTRFPVTED"
BASE COUNT          495 a          869 c          878 g          617 t
ORIGIN      
        1 gatgccctgg gcgacgagct gggtcgtgac cgtggtctcg tcgggggcga cctcaaagag
       61 cagttcgtcg tgtatctgga ggataagccg cgaagagagg cccttgagcc ttgttgccct
      121 gcggatgttg atcatggcca tctttatgat gtccgcggcg gtgccctgaa tgggagtgtt
      181 catcgcgaca cgctcgccga gctggcgaac ggccgggtcg gagttgccca gctccggtat
      241 gaaccgcatc ctcccaagga gcgtcctcac gaaccccttc acccgggcct cctcgataac
      301 gctgtccatg aactccctca cggccctgtg ccgctcaaag tagctgtcga tgtactcctg
      361 ggcctccttc tggcccacgc cgagttcccg ggcgagaccg aaggcgctca tgccgtagat
      421 tatcccgaag ttgatcacct tggcggcgcg ccgcatgtcc tgggtcatgg cgtcggccgt
      481 cacgtgaaag acgtcccggg ccaccgttga atgtatgtcc tcgtcgcgat gaaatgtggc
      541 gatgagttcc cggtcccctg acaggtgggc caggacgcga agctcgatct gcgaatagtc
      601 cgaggacagg atcaagcatc cgtccgcggg cacgaaggcc tcgcggatct tcggcccttc
      661 ctgtccccgt acggggatgt tctggaggtt ggggtcgctg ctcgaaagcc gtcccgtcgc
      721 ggcgaccatc tggttgaagg tcgtgtgtat cctccccgtg agcgggttga tgagcgtggg
      781 caggacgtcg atgtaggtgt tcttcagttt cgtgagcgtg cggtactcga ggatcctgcc
      841 gggcagggga tgactctcgg agagcgtctc caggactgcc gtgtctgtcg agaaccccgt
      901 cttggtcttc ctgacggggg taagcccgag gatctcgaaa agcacccggc tgagttgctg
      961 cggggagtta atgttgaaag gtccgccgca atgggcgtag atgtccttca cgatccccgt
     1021 gagacgctgg tcgatatcgc gcgacaattc accgagcctg cgcctgtcga tcctgacgcc
     1081 agccttctcc atatcggcga gcaccgccac gaggggcaac tcggtaccaa agaaaaggtc
     1141 tgacaggtca agttccccca accgtgcccg gagggcatcg gcaaggtcgg gaagacacgc
     1201 ggcaaggtct cgaagggacg atgacgcatc catcgcggat atgtcggcat cgagatactc
     1261 ctccgctatc ggcccgggcg cgaagtcctt gcgcagaggg ttcgcgagat aggcggcgag
     1321 catggtgtcg aagaccttct tctcggcaag gagatccccc gcgaccacga agagtggttt
     1381 cagatcgtgc cctatcacct ccttcgcgct gcgaaggacc gcggtcaggt cggcgccgtc
     1441 ggcggagaag aagacccctt caccgtcgaa ggcggcaaag gcctcaagcg ccaggtcgcc
     1501 ggcgttcttt ccccggaagg aggccgctat cgacacacgg tccgtcgcaa gttcggagag
     1561 tgctttttcc ctcacatcct tcttctgcgt cccctggacc cggacctcgc ggtaaagggc
     1621 ggtgaactcc agttccctgt acatcctgcg gagggtctcg aggtccggcg gcctttcgat
     1681 caggccctcc acgccggtct cgatggggac gtcgtacctg agcgtggcca gtttcttgct
     1741 catttccgcc ttttcccgtc ctgcaacgag cctttccctg actgccttcc tctttatctc
     1801 gtctaagcgg gcatagatct cctcgacgga tccgagtgag gagacgagct cgcgggccgt
     1861 tttctcgccg atgcccggca cgcccgggat gttgtcggag gtatcgccgc agagcgagag
     1921 gtagtccacg acattccgcg gcgggacgcc gaacttctcg atcacctccg cctcgcctat
     1981 gacgacgttc ttcatggagt cgaggatgcg caccctgtca cccacgagct gcatcatgtc
     2041 cttgtcgccc gtcacgataa aggtcatcac gtcgtccctc gttctcaggt gttccgtgat
     2101 ggtggcgatg atatcatccg cctcgaagcc ctctttctcc acgacgggca gccccatggc
     2161 atcgatgatc tgtttgacga acggcacctg gagggaaaga ttgtcgggca ttgccggccg
     2221 ctgcgccttg tattcccggg atatctcctc cctgaaggaa ggcccccggg agtcgaagac
     2281 gacgatgagg gtatcggggt tctcgctgct cgcgagcttc cggatcatgc tcacgaaggc
     2341 ataaatggcg ttcgtgggaa ccccccgtga gttggagagg tgcggtgtcg cgtagaaggc
     2401 gcggtacaga taggaatgtc catcgacgag gaagacgcgc ttgtatgtcg cggcggatgt
     2461 gttctctgtc atgttccctt cctggcggtc tttagtcttc ggttacaggg aaacgggtcc
     2521 ttatcttgaa ggtccgtttt tcagggaagc gctggatggt gatggagaag gtctcttcca
     2581 gcatcgagag gaacctttct atgtcctcct tcctctccat cgtaacggtg aaccagaggt
     2641 tcagctcccc ttcacgttcg taattgtggg tgacccccgg ctcttcgctc acagtgccgg
     2701 agatctgccc gagtctatca ggaggaatag cgactccgca caggaggctt gtgtacccca
     2761 acctcttggc gtcgagcacc ggcccgatgc gccgtatgcg gcccccgtcc ttgaggtcct
     2821 tcaccctgtt cctggtctct tcctcggtca ggccgatct
//
LOCUS       DAOQTR010000292         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41257_length_2858_cov_2.762397, whole genome shotgun sequence.
ACCESSION   DAOQTR010000292 DAOQTR010000000
VERSION     DAOQTR010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41257_length_2858_cov_2.762397"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..760)
                     /locus_tag="PKN85_07500"
     CDS             complement(170..760)
                     /locus_tag="PKN85_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44293.1"
                     /translation="MVSKRPHVFPGVIFLVLFLFLSGAVVNADPLPAVDPGMKGKTVG
                     AADDLPKVSAVKNPHGFLQFDGTGYRVVTKEVKPERTGHIRLKPVTQPGGSGKGSTVY
                     TLEVLDDRTKEESKQESPASSDGARLAVERVEDLDKKGVAPEPRKEHKADVGLGVKVS
                     ESSEVMVGRALVLERKDDKGLESRDDGWRFRFKTNF"
     gene            821..2281
                     /gene="thrC"
                     /locus_tag="PKN85_07505"
     CDS             821..2281
                     /gene="thrC"
                     /locus_tag="PKN85_07505"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT44294.1"
                     /translation="MGETAYYYSTNNPAERVTFETALLTGMASNYGLYMIARKDIPVL
                     SRGRIEAMKGMTYAGIAYEVLEPFLGREVPPARLRSLLDDAYDARKIPTLVQKVTGQT
                     RIMWLTRGPTYSFKDYAARFFGRMLDHFLGERGLRRVVIVATSGDTGGAVADALLGLK
                     NIDNIVFYPRGSISEGQRRQMTTLGDNVHAFEVNGDFDVCQALAKNILGDASYAFEVF
                     GDRERFTSANSISVGRLLPQAVYPFYAYSQMETSSGPFIASIPSGNFGNMMGTVIARQ
                     MGLPVEKIICGVNENTEFPDFLASGSYAVRPSIKSPSSAMIVSHPSNLARLIDFYGGH
                     MFDERDPSTGNVTRPGIIDRMPDMQAIRRDIVSLAINNPRHFETMKDVYERYGIILDP
                     HGAVGWAALEEFLGGKHGRTAVVYETADPGKFPDDVRKAIGVVPELPPGMKEQAGLPE
                     RIYSIGAEPEKTPAGLKLSPAQVSETKEKIRGIYNR"
     gene            2307..2618
                     /locus_tag="PKN85_07510"
     CDS             2307..2618
                     /locus_tag="PKN85_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNT44295.1"
                     /translation="MVQAATKPIPSFLPLLAILALCLLPTTGCFLGDSIGDIDVGKLE
                     GLMEREQRLLVVDNRTTLEYASGHIPGAMHIPQEEFYRLASLLPPEKDTPIVFYCRGY
                     G"
BASE COUNT          609 a          887 c          785 g          577 t
ORIGIN      
        1 cctgcacgtg gctggtcaga atccccctct gttatctgtt cgtcgtcgtc ctgggattcg
       61 aggcgcacgc cgtgtggtgg accatgaacc tgtcgcaggc cctcgccgcc gcgctgatat
      121 tcagaagata tatgggaaaa aagtggctgc cgggtcttcc ggctcaatcc tagaaattcg
      181 tcttgaagcg aaaccgccag ccgtcgtcgc gggattccag acccttgtcg tcctttcgct
      241 caaggacaag agcgcggccg accatcactt cgctcgactc ggagaccttg accccgagac
      301 ccacgtcggc tttgtgctcc tttcttggtt cgggagctac gcctttttta tcgaggtcct
      361 ccaccctttc aacggcaagg cgggctccat cgcttgaagc gggactctcc tgcttgctct
      421 cttccttcgt cctgtcatcc agcacttcga gggtatagac cgtggagccc tttccgctcc
      481 cccccggctg cgtgacgggc ttgagcctga tatggcctgt cctttccggt ttgacctcct
      541 tcgtcaccac ccggtagccc gtaccgtcga actgaaggaa accatgaggg ttctttactg
      601 ctgacacttt ggggagatcg tctgcggcac cgaccgtctt gcccttcatc ccggggtcta
      661 ccgcaggcaa ggggtccgcg ttgaccacgg ccccgctcag gaacaaaaag agaacgagaa
      721 agatcacgcc ggggaacaca tggggtcttt tcgagaccat tctaggacaa ttataaagga
      781 agttgaaaaa aaatcaaact ttttgtatgc ttaacctgcc atgggcgaga ccgcgtatta
      841 ttacagcacc aataacccgg cggaacgcgt cacttttgag accgcgctgc tcaccgggat
      901 ggcatcgaac tatgggcttt acatgattgc ccggaaggac atcccggtcc tttcgcgggg
      961 ccgcatagaa gccatgaagg gcatgacata cgccgggata gcctacgagg tcctggaacc
     1021 tttcctcggg cgggaggtgc ccccggcgcg gctgagatcg cttctcgatg acgcctacga
     1081 tgcccggaag atccccacac tggtccagaa ggtcacggga cagacgcgca taatgtggct
     1141 cacccgggga ccgacgtatt ccttcaagga ctacgccgcc cggttctttg gcaggatgct
     1201 cgatcacttc cttggagaaa ggggactgcg aagggtcgtc atcgttgcca cgagcggaga
     1261 taccggggga gccgtggccg acgccctcct tggcctcaag aacatcgaca acatcgtctt
     1321 ctatcccagg ggatccatca gcgagggcca gcggcggcag atgacgaccc tcggcgacaa
     1381 cgtccacgcc ttcgaagtga acggcgattt cgacgtctgc caggcgcttg ccaagaacat
     1441 cctcggcgat gcgtcctacg ccttcgaggt cttcggtgac cgggagcgct tcacctccgc
     1501 aaattccatc agcgtgggca ggctccttcc ccaggcggtc tacccctttt acgcgtattc
     1561 gcagatggag acctcttcgg gacccttcat tgccagcatc ccttcgggca acttcggcaa
     1621 catgatgggg accgtcatag cgaggcagat ggggcttccc gtcgagaaga tcatctgtgg
     1681 cgtcaacgag aacaccgagt tccccgattt tctcgcctcg gggagctatg ccgtgcggcc
     1741 ttccatcaaa tcaccgtcat cggccatgat cgtctcccat ccgagcaacc tggcgcgcct
     1801 catagatttc tacgggggcc acatgttcga cgagcgggac ccgtcaacgg gcaacgtgac
     1861 gagacccggc atcatcgacc gcatgcccga catgcaagcc atacggcggg acattgtctc
     1921 cctggcgata aacaatcccc gtcatttcga gacgatgaaa gatgtttacg agcgttacgg
     1981 gattatcctc gacccccacg gcgccgtggg ctgggccgca ctcgaagagt ttctcggcgg
     2041 aaaacatgga cggacggccg tggtttacga gacggccgac ccggggaagt tccccgacga
     2101 cgtccgcaaa gcgataggtg tggtcccgga attgcccccg ggcatgaagg aacaggcggg
     2161 tctccccgaa cgaatttaca gcattggcgc ggagccggaa aagacacccg cgggactcaa
     2221 gctttcaccg gcacaggtga gcgagacgaa ggaaaagatc cgaggaatat acaaccggtg
     2281 aaccaccgtg ttccctgaat aaacacatgg tccaagccgc cacaaaaccc atcccgtcct
     2341 ttctgcccct tctggccata ctcgccctgt gtcttctccc gacgaccggc tgttttctcg
     2401 gcgacagcat cggagacatc gatgtcggaa aacttgaagg tttgatggaa agagagcaga
     2461 gactcctcgt cgtcgacaac aggaccaccc tcgaatacgc gtcaggccat ataccgggtg
     2521 ccatgcacat tccccaggag gaattctaca ggctggcgtc ccttctcccc cctgaaaaag
     2581 acacgcccat cgtcttctat tgcaggggat acggctgagc cccgagcttc aaggccgcgg
     2641 tcgcggcacg gaacgcgggc tacaccaacg tcatgacctt tctggccgga tatcccgcgt
     2701 gggtaaggaa gggtaacccg gtagagcgct aaacatctca aacccgccct tgacggcgtt
     2761 cccccggaat cccgcattga catccccttc tccttgccat agaataaatg gaaacaattt
     2821 ctatttagac ggccatgaag gatcacagcg acattctg
//
LOCUS       DAOQTR010000293         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41732_length_2837_cov_3.826024, whole genome shotgun sequence.
ACCESSION   DAOQTR010000293 DAOQTR010000000
VERSION     DAOQTR010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41732_length_2837_cov_3.826024"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /gene="tilS"
                     /locus_tag="PKN85_07515"
     CDS             <1..517
                     /gene="tilS"
                     /locus_tag="PKN85_07515"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNT44296.1"
                     /translation="SRRPNLRLDLPEHLRVTKTYGNLIFTTKRPAAQVTETFPLGEGR
                     TRIAALGMNVTLTVLKRPPSSYPKDNFTAYFDLDKLDPRDGLAVRTFRRGDRFQPLGM
                     DKPVKIKDFFISRKIPLESRRLVPLLVSGDAIVWVAGHRIDHRYRIDENTRRVLKVTV
                     KKSCQPSSNCD"
     gene            694..1884
                     /locus_tag="PKN85_07520"
     CDS             694..1884
                     /locus_tag="PKN85_07520"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT44297.1"
                     /translation="MLSARAASLKPSPTLAISAKEKALKAQGVDIAGFGAGEPDFDTP
                     VHIKEAGIKAINDNFTRYTPVTGIDPLKDAIIDKFARDNGLAYKREEIIVSCGGKHGL
                     YNLFQALFQEGDEVIVPTPYWVSYPPMIELADAKPVILETREEDDYQITPDLLAKYLT
                     PRTKAIILNYPSNPVGSVFYRENLEAIGKLAVEKGFYLISDEIYEKLVYDDYKHVSIA
                     SMDPAFKERTIICHGVSKTYAMTGWRIGYSAGPAAIIKAMGNIQSQSTSNPTSVSQMA
                     ALAALSGKQDFIGTMVAEFKKRKDFLVKSLRSIEGVTCYDPRGAFYVFPNFNVVMGRK
                     YKDRVVDSSTALTEILLDDFHTAVVPGVEFGNEGYLRLSFATSMEVIEKGVDRITKAV
                     ASLT"
     gene            2017..2289
                     /locus_tag="PKN85_07525"
     CDS             2017..2289
                     /locus_tag="PKN85_07525"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNT44298.1"
                     /translation="MAEKQAKKRVTFKFSDAGAGHVYIAGSFNSWDPSVRPLKKDSKG
                     VWKTSITLSPGAYQYRFIVDGNWMEDPSSGHKEMNEFGGFNSVVIV"
     assembly_gap    2566..2575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2576..>2837
                     /locus_tag="PKN85_07530"
     CDS             <2576..>2837
                     /locus_tag="PKN85_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNT44299.1"
                     /translation="LHEGGLVEKAISNAIRALLERNSDLAEKVIEDDPVINALEVEID
                     EFCLKLLALRQPAARDLRFITTAIKINYDLERIGDMAVNICER"
BASE COUNT          697 a          849 c          749 g          532 t
ORIGIN      
        1 ctctcgaaga ccgaacctgc gtctcgatct ccccgaacat ctcagggtaa cgaagactta
       61 cggcaacctc atattcacaa cgaagaggcc ggcggcacag gtcacggaaa cctttcccct
      121 cggcgagggg aggacccgga tcgccgcgct tgggatgaac gtcacactca ctgtattgaa
      181 gaggcctcct tcatcctacc cgaaggacaa tttcacggca tacttcgacc tcgacaagct
      241 agatccccgg gacggcctag cggtccggac ctttcgccgg ggtgaccggt tccagcccct
      301 cggcatggac aagccggtca agataaagga tttcttcata tcccgcaaga tacccctcga
      361 gtcccgcaga ctggtccccc tcctcgtctc aggcgacgcc atcgtctggg tcgcgggcca
      421 caggatcgac catcggtaca ggatagacga gaacacccgc agagtcctga aggtcactgt
      481 gaaaaaatcc tgtcaaccat cttcaaattg tgattgacag agccttccct catggtgtaa
      541 aagttaccgc tgtacccata cgggatcagc agggagtgag gagcaaggag cagggagtga
      601 ggagtcctta aacaagaaga tccggtgagg cttcgcgatt ccttcccttt ttctgttcac
      661 tctgcaccca aaaaaagcta aaggagttcc gttatgctat ccgcaagagc cgcctcactg
      721 aaaccgtcac ccacactcgc catctcggca aaggagaagg ccctaaaggc gcagggggtc
      781 gatatcgccg gcttcggcgc cggcgagccg gacttcgaca cccccgttca tatcaaggaa
      841 gcgggcataa aggccatcaa cgataacttc acccgctata ctcccgtcac cggcatcgat
      901 ccccttaagg acgcgataat agacaagttc gcccgcgaca acgggcttgc atacaagcgg
      961 gaagagatca tcgtttcctg cggcggtaaa cacggcctct acaacctttt ccaggccctt
     1021 ttccaggaag gtgacgaggt tatcgttccc accccctact gggtatccta cccccccatg
     1081 atcgaactcg cggatgccaa acccgtcatc ctcgaaacca gggaggaaga cgactaccag
     1141 atcaccccgg acctcctcgc gaaatacctt acgcccagga cgaaggcgat catactgaac
     1201 tacccttcga accccgtcgg ctccgtcttc tatcgggaga acctcgaggc aataggcaag
     1261 ctcgcagtgg aaaagggttt ctacctcatt tccgacgaga tatacgaaaa actcgtctat
     1321 gacgactaca aacacgtcag catcgcctcc atggaccccg cgttcaagga gcggacgatc
     1381 atatgccacg gcgtctccaa gacctacgcg atgacgggct ggaggatcgg ctacagcgcg
     1441 ggccccgccg ccatcataaa ggccatgggg aacatccaga gccagagcac ctccaacccg
     1501 acctccgtgt cgcagatggc ggccctcgca gccctttccg gcaagcagga cttcatcggc
     1561 accatggtcg ccgagttcaa gaagagaaaa gactttctcg ttaagagctt aaggagcata
     1621 gaaggcgtga cctgctatga tccccggggc gccttctacg tgttccccaa cttcaatgtc
     1681 gtcatgggca ggaagtacaa ggaccgcgtt gtcgattcct caacagctct gacggagatc
     1741 cttcttgacg acttccacac cgccgtggtc cccggtgtgg agttcggcaa tgaaggatac
     1801 ctgaggctgt ccttcgccac gtccatggag gtcatcgaga agggtgtcga cagaataaca
     1861 aaggcagtgg catccttgac ctgaacatag agaacatgcc tcggaaaggg ggaaccgatg
     1921 cgggctcacg cacggttccc cctttccgtt cccccggccc ggatgaaaaa tccgggggat
     1981 tgtgatatta tgatggacaa aagcaaggag ggtgcaatgg cagagaagca ggcgaagaaa
     2041 cgtgtaacat tcaaattcag cgacgcgggg gcaggccacg tttatatcgc aggttctttc
     2101 aactcctggg acccgtcggt tcgccctctg aagaaggact caaagggggt ctggaaaacg
     2161 agcatcaccc tttccccggg tgcctaccag taccggttca tcgttgacgg caactggatg
     2221 gaagacccct cttccggcca taaagagatg aacgagttcg ggggtttcaa ctccgtggtc
     2281 atcgtctgat caccgtgtca gagccgcgta ggtcccgaac aacacgtaaa ccccggtgac
     2341 ggcaggagcg gtctcaccgg ctcacatcca tttcaagcct atcgagcgat ccgaaacatg
     2401 gaggcggcgc cgggccttgc cctgcatgtt gtcaggggtc tcagacgcct caaaatcaga
     2461 ttgtaatcga tggcaaagtt gactatactc tacccatcca gaccaggagg cgagcaatgc
     2521 ttgaaggaca catctacaag actttcgatc ttgagctgaa agagcnnnnn nnnnnctcca
     2581 tgaaggaggg ctcgtcgaga aggccatcag caatgccatc agggcccttc ttgaacgcaa
     2641 ctcggacctt gccgagaagg tcatagagga tgacccggtc atcaatgccc tcgaggtgga
     2701 gatcgacgag ttctgtctca agctcctggc actcaggcag cccgcggccc gcgacctgag
     2761 attcatcacc acggccatca agatcaacta cgacctggaa cggatcggcg acatggccgt
     2821 caacatctgc gagaggg
//
LOCUS       DAOQTR010000294         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41785_length_2835_cov_3.314029, whole genome shotgun sequence.
ACCESSION   DAOQTR010000294 DAOQTR010000000
VERSION     DAOQTR010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41785_length_2835_cov_3.314029"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1057
                     /locus_tag="PKN85_07535"
                     /pseudo
     CDS             95..1057
                     /locus_tag="PKN85_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611518.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1169..2359
                     /gene="istA"
                     /locus_tag="PKN85_07540"
     CDS             1169..2359
                     /gene="istA"
                     /locus_tag="PKN85_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNT44300.1"
                     /translation="MLRKEDWMDIKEQVERGMYRKDVAERLAVSPRTIQRALRRGGPP
                     KGDRPGAKKSKLDPFTPVIDDLLRQGVWNAVVILRELRARGFTGECTIVRDYIRPKRP
                     LREGRQTVRFETPPGRQMQSDWGEIVTIVAGRPQRVFFTVNTLGYSRRFHFLCTDRND
                     AEHTYEGMIRSFEHFGGVPSEVLVDNQKSAVIEHRVGNAVRFNERFLDLAGHYGFTPR
                     ACRPYRARTKGKDERMVGYIKHHFFVRYRAFESLSHMNECALLWLAEEADVRMHGTVK
                     EIVRERFAREAPCLGPLPSIRYDTSYREQRLAHWDGYIDVRGNRYSVPAHLCGRSVNI
                     RITLDDDLLIYDNAIPVARHRLRHATDGWVTDNDHHRDLWRNTLKVERRALAVYEEVI
                     PCSL"
     gene            2347..>2835
                     /locus_tag="PKN85_07545"
     CDS             2347..>2835
                     /locus_tag="PKN85_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44301.1"
                     /translation="MQLVSLLEKMKMDHISGQLDALCEQAAKKDMNYRDFLTDVLQTE
                     WTGRHQKGVEARLKLARFPWMKTMEQFDFSFQPTIDRKVIRELSSVAFVERGENIIIL
                     GPPGVGKTHLSVALGVKALEAGHTVLFLTLESMITRLTRARMENRVERQLIQFVSPKV
                     LII"
BASE COUNT          675 a          801 c          824 g          535 t
ORIGIN      
        1 cctcgccaaa aggggaaaac ggaggatacg tcttatgaag aataatgaac ctgtttacgg
       61 aaaatagcaa gagaaagaag aaccgcaatc ggtactggag gagatcatca gggaaggggc
      121 aaggaaattg ctccagacag cgatagagaa tgaagtggcg gaatacatcg ccctgtactc
      181 ggagagaaga gatgaaaagg gcagacgcat ggttgtccgt aacggtttcg ccccggagcg
      241 ttccatcctc accggcatcg gctccatatc gataagacag cccaggatac gggacagaag
      301 ggagaaaaaa gaatccttca ccagttccat ccttccccgc tatctgcgcc gcgtcccctc
      361 cctcaataat ctcatacccg tcctttacct caagggcgtc tccaccggcg acttcaccga
      421 tgccctgtcc gccattctcg gcgagaacgc gaaagggctc tccgcgaaca ccgttgtccg
      481 tctcaagagg cagtgggagg aggaataccg gcagtggacg aagcgttctc ttgaggggaa
      541 aagatacgcc tacttctggg tggacggcat caacttcaat gtccatcttg aggacccgga
      601 aaacaaaagg cagtgcatgc tcatcattat cggcgccggc gaggacggca ccaaggaact
      661 cgtgggaatc ctcgacggat accgggagag caagctctcc tggatggagc tgttgtccga
      721 tctcaagcaa gggggtcttg ccgaaggacc agcccttgcc ataggcgacg gtgccctggg
      781 tctctgggta gccctctagg aggtgtatcc ggacacaaaa gagcaacggt gctgggtgca
      841 taaaacagcg aacatccttg acaagatgcc aaaaagcgtg cagccgaagg caaaggaaag
      901 aatccatgac atctatatgg tccccacaga gggacagacc cttgtcgcct ataatgcatt
      961 cctctccctc tatgggacaa agttcgggaa ggcctgcgag tgtcttatga aggacaaagg
     1021 ggttctcctg tccttctatg acttccctgc ggagcactgt gtcatcaacg gtgttatatg
     1081 tcttataatc agggaaataa aatgtcctct catataagcg atctgatata ccctcaatac
     1141 aaagaacatc gttccggagg gtatgcctat gctcagaaag gaggactgga tggatatcaa
     1201 ggaacaggtt gaaaggggta tgtatcggaa ggacgtagcg gagcgtctcg ctgtaagccc
     1261 aaggaccatt cagagggcgc tgagaagagg cggcccgccg aaaggtgaca ggcccggggc
     1321 aaagaagagc aagctcgatc cctttacgcc cgtcatcgac gacctcctcc gtcagggggt
     1381 ctggaatgcc gtcgtcatct taagggagct tcgggcacgc ggttttaccg gtgagtgcac
     1441 catcgtcaga gactacataa ggcccaagcg cccattgcgc gagggaagac agaccgtccg
     1501 cttcgagacg cccccgggca ggcagatgca aagcgactgg ggagagatcg tcaccatcgt
     1561 cgcagggagg ccgcagaggg tcttctttac cgtcaacacg ctgggctatt cccggaggtt
     1621 ccacttcctc tgcaccgacc gcaatgacgc cgagcacacc tacgagggga tgatcagatc
     1681 attcgagcac ttcggcgggg tgccctccga ggtcctcgtg gataaccaga aaagcgccgt
     1741 catcgagcac cgtgtgggca atgccgtcag gttcaacgaa cgcttcctcg acctcgccgg
     1801 ccactacggg ttcaccccca gggcctgccg cccctaccgt gcccggacca agggcaagga
     1861 cgaacggatg gtggggtata tcaaacacca cttcttcgtc cgctaccgcg ccttcgagag
     1921 cctctcccat atgaacgagt gcgcccttct ttggcttgcc gaagaggcgg atgtgaggat
     1981 gcacggaacc gtcaaggaga tcgtccggga gcgtttcgcc cgtgaggcac cctgtcttgg
     2041 tccccttcct tccatccgct acgacacctc ctaccgggaa cagaggctcg cccactggga
     2101 cggctatatc gacgtgaggg ggaaccgcta cagcgtcccc gcgcacctgt gcggcaggag
     2161 cgtgaacatc cgcatcaccc tcgatgatga tcttcttatc tacgacaatg ctatccctgt
     2221 ggcccgtcac cgtctccgtc acgcaacaga cggatgggtc accgataatg atcatcaccg
     2281 ggatctctgg aggaacaccc tgaaggtgga acggagagcc ctcgccgtct acgaggaggt
     2341 gatcccatgc agcttgtgag cctccttgag aagatgaaga tggatcatat aagcggccag
     2401 ctcgacgcgc tgtgcgagca ggcggcgaag aaagacatga actaccggga ttttctcacc
     2461 gacgttttac aaaccgagtg gacgggccgc caccagaagg gggtcgaggc gaggctcaag
     2521 cttgcccgtt tcccctggat gaagacgatg gagcagttcg acttctcctt ccagcccacc
     2581 atcgaccgga aggtcatccg ggagctctcc tcggtcgcct ttgtggagcg gggggagaac
     2641 atcatcatcc tggggcctcc cggcgtgggg aagacgcacc tctccgttgc cctgggggtg
     2701 aaggcattgg aggcgggcca cacggtgctc tttcttaccc ttgagtcgat gattacccgc
     2761 ttaacgaggg cgaggatgga gaaccgggtg gagaggcagc tcatccagtt cgtctccccg
     2821 aaggtcctca tcatc
//
LOCUS       DAOQTR010000295         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_41809_length_2834_cov_3.502699, whole genome shotgun sequence.
ACCESSION   DAOQTR010000295 DAOQTR010000000
VERSION     DAOQTR010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41809_length_2834_cov_3.502699"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKN85_07550"
     CDS             <1..347
                     /locus_tag="PKN85_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44302.1"
                     /translation="LLAELAHDILESLGYRVTAVTDSTQALRVFSSDPASFDLVITDQ
                     IMPGFTGLNLAKHFLDIREDIPIILCTGHSATVSPEIAKEAGIRAFVMKPLSKEDLAE
                     VIRAVLDVGTVQ"
     gene            478..1428
                     /locus_tag="PKN85_07555"
     CDS             478..1428
                     /locus_tag="PKN85_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT44303.1"
                     /translation="MKVHFWGTRGSLPAAASTLDLKRKVFGAIKSLAGRHLAGDDDVR
                     EFLEKELPFHLAGHYGTNTPCVEIRGGTEHVICDAGTGIRDLSRTLGVAPDGDPGERV
                     FNIFLSHLHWDHIQGFPFFMPAYSEGSRIVVYGGHPDMKAALDYQRGYSNMPLPVKAD
                     LSFRRLEPGRQYEIAGFQVLVASQNHPGDSYGYRFEVEGGSVVYSTDSEHLEEGSADE
                     RRFIEFFRNADLVIIDAQYPLLDAFSVKENWGHSNNIVAVELGAKAGVRRLVLFHNEP
                     TVDDHGLETFLNDTRRYLEIYDPGNPMQIDLAYDGMEIEI"
     gene            complement(1553..2239)
                     /gene="gpmA"
                     /locus_tag="PKN85_07560"
     CDS             complement(1553..2239)
                     /gene="gpmA"
                     /locus_tag="PKN85_07560"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNT44304.1"
                     /translation="MTRIVLLRHGESQWNLENRFTGWTDIDLSERGVEEARQAGRALR
                     EAGFIFDVAFTSVLKRAIRTLWIVLEELDRMWIPQRKSWRLNERHYGALQGLNKAETA
                     VEYGEEQVRLWRRSYDVPPPPLERRDKRFCGNDPRYDALAPEELPVSESLKDTAARVM
                     PYWEGTIVPVLARGARIIISAHGNSLRSLVKHLDNIPDEDVPALEIPTGKPLVYEFSQ
                     DLKPTKHYYL"
     gene            complement(2259..>2834)
                     /locus_tag="PKN85_07565"
     CDS             complement(2259..>2834)
                     /locus_tag="PKN85_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNT44305.1"
                     /translation="VSRETYTYITAEAARLGFPVASLDRQRPWFLAMTMSSIELMKAG
                     YNPHYGIDKYFLTRAAGRKKLVELETIDYQIDLLARLPDKEQESFLLYALKDLSSLVQ
                     QVDRLMAAWTTGDSDLIAELLEKSVGEDESLKVIHRKILTERNRNMAKKIALHLGSSG
                     CVFVVVGAAHLVGDKGIVELLRKEGFTVEQL"
BASE COUNT          535 a          814 c          837 g          648 t
ORIGIN      
        1 acctccttgc cgagttggcc cacgatattc tggagtcgct cgggtaccgg gtcaccgcgg
       61 tgacggacag tacccaggcg ttgagggtgt tctcgtccga tcccgcctct ttcgatctcg
      121 ttattaccga ccagatcatg ccggggttca ccggtctcaa tctggcaaaa cacttcctgg
      181 atatacggga ggatatcccc atcatcctct gcacgggtca cagcgcgacg gtatcgccgg
      241 agatcgcgaa agaggccggc atcagggcgt tcgtgatgaa gcccctttcg aaggaggacc
      301 tggcggaggt catccgagcc gtgctcgatg ttggaaccgt gcagtgacgc tcagagggtg
      361 tgactgcagt tctgtttcgg tccggtagct tcccggattg tccttgtaat ttcgcttctc
      421 cacgactatt gtattggtat tccgggctgc gcatcgtggc cgggacggag gttcctgatg
      481 aaggtgcatt tctgggggac gcgggggtcg ctgccggcgg ccgcgagcac gctggacctg
      541 aaacggaagg tgttcggggc cataaagagt cttgcggggc gacatttggc aggcgatgac
      601 gatgtccggg agttcctgga gaaagagctc ccctttcacc tggcgggtca ctatggcacg
      661 aacacgccct gtgtcgagat aaggggtgga acggagcatg tgatctgcga cgccggtacg
      721 gggatccggg acctttcgcg cacgcttggc gtcgcgccgg acggcgatcc gggcgaaagg
      781 gtcttcaaca ttttcctgtc ccacctgcac tgggaccata tccagggatt tccgttcttt
      841 atgcctgctt actcggaagg gagcaggatc gtggtctacg gcggccatcc cgacatgaag
      901 gctgccctcg actaccagag gggatattcc aatatgcctc ttcccgtgaa ggcggacctg
      961 agctttcgca ggcttgagcc gggccggcag tacgagatag cgggtttcca ggtgctggtc
     1021 gcatctcaga accatcccgg cgattcctac ggctaccgtt tcgaggtcga gggcggaagc
     1081 gtcgtctact ccaccgacag cgaacacctg gaagaggggt ctgccgacga gcgccggttc
     1141 atagaattct tccggaatgc cgaccttgtt atcatcgatg cccagtaccc actactcgat
     1201 gccttttccg tgaaggagaa ctggggccat tccaacaaca tcgtcgccgt cgaactgggg
     1261 gcgaaggcgg gagtcaggcg tctcgtcctc tttcacaacg aacccaccgt ggatgaccat
     1321 ggccttgaaa ccttcctcaa cgacacgagg cgttacctcg agatctacga ccccggaaac
     1381 ccgatgcaga tcgacctggc ctacgacggg atggagatag agatttaaag accgtgttaa
     1441 gttttaagtt attaagtttt aagttaaaga ctttttttct ttcacctaaa acctgaaact
     1501 taacaccgtt tctttttttc tttcacctaa aacctgaaac ttaacaccgt ttctaaagat
     1561 aataatgttt cgtcggtttc aggtcctgtg agaactcgta gacgaggggt ttgcccgtgg
     1621 gtatctcaag ggcggggacg tcctcgtcgg ggatgttgtc gaggtgcttg acgagagagc
     1681 gcaggctgtt gccgtgggcc gatatgatga tcctggcgcc ccgcgcgaga acggggacga
     1741 tggtcccctc ccagtagggc atcacgcgag cggcagtgtc tttcaggctt tcggagacgg
     1801 gcagctcttc cggggcaagg gcgtcgtacc gggggtcgtt gccgcagaaa cgcttgtccc
     1861 gtcgctcaag ggggggaggg ggcacatcgt aactgcgccg ccacaggcgg acctgttctt
     1921 ctccgtactc gaccgccgtc tccgccttgt tgaggccctg cagggccccg taatggcgtt
     1981 cgttcagtct ccaggatttg cgctgtggta tccacatgcg gtcgagctct tcgaggacga
     2041 tccagagggt ccgtatggcc ctcttcagca ccgatgtgaa ggccacgtcg aagatgaaac
     2101 cggcctctct cagcgcacgt ccggcctgcc gggcctcttc aactcccctc tcggagaggt
     2161 cgatatccgt ccagcccgtg aacctgttct ccaggttcca ctggctttcc ccatgccgca
     2221 gcaaaacgat ccgtgtcatg tatgacgctc cttcattatt ataattgttc caccgtgaac
     2281 ccctcttttc tcagaagctc cactatccct ttgtcgccca cgaggtgtgc cgctcccacg
     2341 accacgaaca cgcatcccga ggatccaagg tgcagggcga tcttcttcgc catgttcctg
     2401 ttgcgttccg tcaggatctt ccggtggatc accttcaggc tctcgtcttc acctaccgac
     2461 ttctcgagga gctcggcgat caggtcgctg tcacccgttg tccaggcggc catcagtctg
     2521 tcgacctgct gcacgaggga gctgaggtct ttcagcgcat agaggagaaa agactcctgc
     2581 tccttgtcgg ggagccttgc cagaaggtcg atctggtagt cgatggtttc cagctccacg
     2641 agctttttcc tgccggccgc ccttgtaagg aagtacttgt cgatgccgta atgcggattg
     2701 taaccggcct tcatgagctc tatggaggac atcgtcattg cgaggaacca cggtctctgc
     2761 cggtcaaggg atgcgacagg gaacccgagg cgcgcggcct cggcggtgat gtaggtgtat
     2821 gtctcgcgcg acac
//
LOCUS       DAOQTR010000296         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_42117_length_2819_cov_3.697902, whole genome shotgun sequence.
ACCESSION   DAOQTR010000296 DAOQTR010000000
VERSION     DAOQTR010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42117_length_2819_cov_3.697902"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..912)
                     /gene="budA"
                     /locus_tag="PKN85_07570"
     CDS             complement(67..912)
                     /gene="budA"
                     /locus_tag="PKN85_07570"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNT44306.1"
                     /translation="MGSEKITELEFFLSYDILTGLTGAMERTGCSAADIIEQALRQFL
                     SAKREGNAVYLSAPINALVEGLYVENTTIGEVRQHGDFGLGTFNYLDGEMLLLDGKVY
                     QIRSDGNVYDVGDDERSPFACVTFFSPDTFDDINGDSDVPDLATLLDRLIPSRNMLYA
                     IRVDGVFSYVRCRAASRSENYMPLVEATRDQSIFDFHDVRGSLAGFYTPAFMASLNAP
                     GFHLHFLAEDRRRGGHLIDCTLKESRIGIQHVPRLDVGLPVTLDYLTVDLTRDTGEDL
                     EKAEK"
     gene            complement(902..2257)
                     /gene="pcnB"
                     /locus_tag="PKN85_07575"
     CDS             complement(902..2257)
                     /gene="pcnB"
                     /locus_tag="PKN85_07575"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNT44307.1"
                     /translation="MEPRIVPRKEHRISRKHVSPNALRTLYRLHSRGFIAYLVGGCVR
                     DLLLGRTPKDFDIGTNATPGQIKRLFHNCRLVGRRFRLAHLRFQDEIVEVSTFRRSAD
                     PSDAVEVDEANQGRRHVRHMKDENGMVLADNIFGTPEEDALRRDFTINALAYNIADAS
                     VVDYCTGLTDLKDRLIRSIGDPQVRFTEDPVRMLRAVRFAASHGFDIERTAWECIRQL
                     CSTISRVSPSRLYEEVQKLFLLGFACPTFALLEKSGLLVSLFPDLAGWARERDGCFEA
                     LERHLAGIDELVRSGQRASVALLLAALFGPRLEEAAMARHREGIPRRQALDETCAGFL
                     KEISRGVQIPGRVGARLNAILALKTSLCRMPPRRPATLAGRPEFAETVMHLKTAAGTE
                     GEHQAALTWWKAFLLENPPAESAEAPADEAPRKKRRRRRKKPRRGRSSVVREEGTAHG
                     Q"
     gene            complement(2348..>2819)
                     /locus_tag="PKN85_07580"
     CDS             complement(2348..>2819)
                     /locus_tag="PKN85_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44308.1"
                     /translation="QVSWQCVDPQRRIVRINWSNGKYLDTLTISADGKRMEGKNQLGS
                     RVWAVRHGGQTAAAQRTPAQGAAVKINEKCAPIIGTWRSEQNGRTIVVTADGRLNGNP
                     RYTWSCHPDGVRYNFRWDDGARVVVWDDLRMDPDKMHLTQRQAGQVWDRFVKIR"
BASE COUNT          540 a          860 c          862 g          557 t
ORIGIN      
        1 tcagctgctc ttccgagggg cagtccatct tcactatccg gaaggtggtc ttcaacccgg
       61 tgcctgtcat ttctcggctt tttccaggtc ctcgcctgta tcccgcgtga ggtccacggt
      121 aagataatcc agagtgaccg gcaggcccac gtccagccgc ggcacatgtt gaatgcctat
      181 gcgggactct ttgagcgtgc aatcgatgag gtgcccacct cggcggcggt cttcggccag
      241 gaaatggaga tgaaagccgg gggcattgag ggatgccatg aacgccgggg tgtagaaacc
      301 ggcaagagac cctctcacat cgtggaaatc aaagatggac tggtcgcggg tggcctcgac
      361 gaggggcatg taattctccg acctcgaagc cgcccggcac cgcacgtagc tgaagacacc
      421 atccacacgg atggcgtaga gcatattccg ggagggtatg agcctgtcga gaagggtggc
      481 aaggtccggg acatcggaat ctccgttgat gtcgtcgaaa gtatcggggc tgaaaaaggt
      541 cacgcaggca aagggcgatc tctcatcgtc gcccacgtcg tacacattcc cgtccgaacg
      601 tatctggtac actttgccgt cgagaaggag catctccccg tccaggtagt tgaacgtccc
      661 gagaccgaaa tccccatgct gcctgacctc gcctattgtg gtgttctcaa cgtaaaggcc
      721 ctccacgagc gcgttgatgg gggccgaaag atagacggcg ttgccttctc ttttcgcgct
      781 gaggaattgt cttaacgcct gttcgatgat gtcagccgct gaacaccccg ttcgctccat
      841 cgcacctgtg agcccggtca ggatatcata tgacaaaaag aactcgagct ccgtgatctt
      901 ctcactgccc atgcgcggta ccctcttccc gaacaacgct gctcctcccc cggcggggtt
      961 tctttcgcct ccgccttcgt ttctttcggg gagcctcgtc cgctggtgcc tctgccgatt
     1021 ccgccggagg attctcgagg agaaaggcct tccaccacgt gagagccgcc tggtgttctc
     1081 cctcggttcc ggcagcggtc ttgaggtgca tgactgtttc ggcaaactcg ggtctgccgg
     1141 caagcgtggc gggtctgcgg ggcggcatcc tgcacaggga ggtctttagg gcaaggatcg
     1201 cgttcaacct ggcgcccaca cggccgggga tttgaacacc ccgcgaaatc tccttgagga
     1261 aaccggcgca ggtctcgtcc aacgcctgac gcctggggat accctcccgg tgacgggcca
     1321 ttgcggcctc ctccagccgg ggaccgaaga gtgcggccaa aagcagcgcc accgatgccc
     1381 tctgcccgct tcggaccagt tcatcgatcc cggcaaggtg tctctcgagc gcttcgaaac
     1441 agccgtcacg ctcccgggcc cagccggcaa ggtccggaaa aagagacacg agaagcccgc
     1501 tcttctccag gagcgcgaag gtcgggcatg caaaaccaag gaggaagagt ttctgtacct
     1561 cctcgtagag ccgtgatgga gagacacggg atatggtgga acacagctgg cggatgcatt
     1621 cccatgcggt cctctctatg tcgaagccat gggacgcggc gaacctcacg gcacggagca
     1681 tccgaacggg gtcttccgta aaacgcacct gcgggtcgcc gatggagcgg atgagccggt
     1741 ccttcaggtc ggtgagcccc gtacagtaat ccacgacgga agcgtccgcg atattgtatg
     1801 ccagggcgtt gatggtaaag tcgcggcgga gtgcgtcctc ctcgggtgtc ccgaagatgt
     1861 tgtcggcgag gaccatcccg ttctcatcct tcatatggcg gacgtgtcgc cgtccctggt
     1921 tcgcctcgtc gacctcgact gcatccgaag gatcggcgga acgacggaaa gtggaaacct
     1981 ccacgatctc gtcctggaaa cgcaggtggg ccagtctgaa ccggcggccg acgaggcggc
     2041 aattgtgaaa gagcctcttg atctggcccg gcgtggcgtt cgtgccgata tcgaaatcct
     2101 tcggtgtccg ccctaggaga aggtcgcgga cgcaaccgcc cacgaggtag gctatgaagc
     2161 cgcgggaatg aagccggtag agggtgcgaa gggcgttcgg gctcacgtgc ttgcgcgaga
     2221 tgcggtgctc tttacgcggc acaatccttg gttccatgcg catcaagata ccatatggct
     2281 acgaaaaagc aaagtaagac atcacctggg gacgctgctg aaagcgctgt ttcttccagc
     2341 gaccggatca gcgtatctta acaaatcggt cccacacctg cccggcctgc cgctgggtca
     2401 ggtgcatctt gtcgggatcc attctcagat cgtcccagac gacaactctc gcgccgtcgt
     2461 cccaccggaa attgtaacgg accccgtcgg gatggcagga ccacgtatac cggggattgc
     2521 cgttcaagcg cccgtccgcc gtgacaacga tggtccgccc gttctgctcg gagcgccatg
     2581 tgccgatgat cggcgcgcac ttctcgttga tcttgacggc ggcgccctgc gcgggtgtcc
     2641 tctgcgcggc agcggtctgt cccccgtggc gcacggccca gacacggctg ccaagctggt
     2701 tctttccctc catgcgcttg ccgtcggcgg agatggtgag cgtatcaagg tacttgccgt
     2761 tgctccagtt gatacggacg atccgccgct gggggtcgac gcactgccag gacacctgc
//
LOCUS       DAOQTR010000297         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_42243_length_2813_cov_4.255983, whole genome shotgun sequence.
ACCESSION   DAOQTR010000297 DAOQTR010000000
VERSION     DAOQTR010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42243_length_2813_cov_4.255983"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..640
                     /locus_tag="PKN85_07585"
     CDS             101..640
                     /locus_tag="PKN85_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT44309.1"
                     /translation="MTTNTIFSGFGGQGVLSMGFTLASAAMLEGRYVTYLPSYGVEVR
                     GGTANCTVVVSDEEIASPVASEPEFVVAMNQPSFNRFQNILQSGGLMCVNASLVNASS
                     VRSDIEVLSIPTSELAEQLGTIKVANMIMLGALIRASNIISFDAMIKNLAELLGEGKS
                     KLIKLNREALERGFNYVKE"
     gene            646..1074
                     /locus_tag="PKN85_07590"
     CDS             646..1074
                     /locus_tag="PKN85_07590"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT44310.1"
                     /translation="MVITQLSVSLENVPGALSHVSELLGREGVNIRAISVADTSDIST
                     VRFVVDDPEKAKNILKGNGFNPRETGVLAVETPDHPGGLLAVLKPLKAAGINVHYLYP
                     HLGRVSGNAIVILGVDRTEEAQKVLAQNWVKTLGKEVYSI"
     gene            complement(1203..1820)
                     /locus_tag="PKN85_07595"
     CDS             complement(1203..1820)
                     /locus_tag="PKN85_07595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNT44311.1"
                     /translation="MRAERILWVCVACVLFAAVLRPIGTAHGGSAAFRDTIYDPGRLK
                     PLDSNPTLKVGDVAPDFTLPSITGNPVSLHDYRGRANVVISFVPAAWTPVCSDQWPGY
                     NIMKGLFDRNDAILLGITVDNIPTLYAWTRQMSEKGLWFPVLSDFYPHGAVASKYGIL
                     RTNGESERALFVIDRKGVIRYIDVHDINKRPRLEDLARALQNLQN"
     gene            complement(1831..2436)
                     /locus_tag="PKN85_07600"
     CDS             complement(1831..2436)
                     /locus_tag="PKN85_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT44312.1"
                     /translation="MSIKKLIVLVCAAAALYACFQLFPGGTAALAQTTQLDYSDRRMP
                     LPAFELPIPQDRAEREYLGLSDKGTFKVGDIKSQIVIIEIFSFYCPICQVHAPLVHEV
                     YEKIKARADLKEKIRMIGIAATNTAFEARSFKETYNIPFPVFSDEEGEIALLLGVKYT
                     PTFIGVKVDGKGGQEQFYFLPKVFTNPTRFIEDIMKTSGLE"
     gene            complement(2574..>2813)
                     /locus_tag="PKN85_07605"
     CDS             complement(2574..>2813)
                     /locus_tag="PKN85_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44313.1"
                     /translation="EEIARAQEMKKRAQEGRARAMAAGGTSTDRPQYPWVTMEKAADK
                     MIADALKTIERCDLQIKQGRALKVEAENELKKLGQ"
BASE COUNT          571 a          810 c          811 g          621 t
ORIGIN      
        1 cctgtcccac aaactggaag atggggcccg tcgaggcatg ccgatggata gacgaggtga
       61 tgacgaagca gttccccctg ggactggtaa aagaggtctc atgacgacta acacgatatt
      121 ttcaggtttc ggcgggcagg gcgttctctc catggggttt acactcgcga gcgccgcgat
      181 gctggagggc agatatgtca cctatcttcc gtcctatggc gtggaggtgc gcggcggcac
      241 ggcaaactgc accgtggtgg tctccgacga ggagatcgct tcgcctgtgg catcggagcc
      301 ggaatttgtc gttgccatga accagccttc cttcaaccgc ttccagaaca tcctccaatc
      361 agggggtctc atgtgtgtca acgcctctct cgtgaatgcc tcatcggtgc gaagcgacat
      421 agaggtcctt tccatcccca cgagcgaact cgcggagcag ctgggaacca tcaaggttgc
      481 caacatgatc atgctcgggg cgttgatcag ggcgagcaac atcatatcct tcgacgccat
      541 gatcaagaac cttgccgagc ttctcggcga agggaagtca aaactgatca aactgaacag
      601 ggaggccctc gaacggggct tcaattacgt caaggagtaa tgacaatggt catcacgcag
      661 ttatccgtca gtctcgagaa tgtacccggc gcgctttcgc acgtgagcga gctcctcggt
      721 cgcgaagggg tgaacatccg cgccatctcc gtggccgata catcggatat cagcaccgtc
      781 cgtttcgtcg tagacgaccc cgaaaaggcg aagaacattc tcaagggcaa cggtttcaac
      841 cctcgtgaga caggagtcct tgctgtcgag acccccgacc acccgggagg tctgcttgcc
      901 gttctcaagc ctctcaaggc cgccgggata aatgttcact atctctatcc ccacctggga
      961 agggtgtcgg gcaacgccat cgtcatcctc ggggtggaca ggaccgaaga ggcccagaag
     1021 gtgctggccc agaactgggt caagacgctg gggaaagaag tctacagcat ctgaagaagt
     1081 cccaggtcac aggtttcagg tctcaggaca ataagacggt tgcgcgttgc gagttgcggg
     1141 ttagaggcca gtaaaggaca gcaaggcgca aggatgccga ccttctctcg tgatacatcc
     1201 cgctagttct gcaggttttg gagtgccctt gccaggtctt cgaggcgggg tcgcttgttt
     1261 atgtcgtgga cgtcgatgta tctgatcacg cccttcctgt cgattacgaa gagggcccgt
     1321 tccgattccc cgttcgtacg aagaatgccg tatttcgagg cgacagcgcc gtggggatag
     1381 aagtcggaga ggaccgggaa ccacagtccc ttctcggaca tctgccttgt ccaggcataa
     1441 agcgtgggaa tattatcgac ggttatgccg agaagtatcg catcattcct gtcgaaaagg
     1501 cccttcatta tgttgtagcc gggccactgg tcggagcaga ccggtgtcca ggctgccggc
     1561 acgaaggaaa tgaccacgtt ggcgcggccc cggtaatcgt ggagcgaaac agggtttccc
     1621 gttatggatg gaagcgtgaa atccggggcg acgtcgccca ccttcaacgt tgggttggag
     1681 tccagcggct tgagccttcc agggtcgtag atagtgtctc tgaaggccgc ggaccctccg
     1741 tgtgccgtcc cgatcgggcg caggaccgcg gcgaagagga cgcatgcgac acaaacccac
     1801 aggatgcgct ccgcccgcat tgtctggctc ctattccagt cccgatgtct tcatgatgtc
     1861 ctcgatgaac cgggtagggt tcgtaaaaac cttcggcagg aagtagaact gttcctgccc
     1921 tcccttgccg tccactttca cgcctatgaa ggtgggtgtg tacttgacgc cgagaagaag
     1981 cgcgatctcc ccctcttcat cggaaaagac cgggaagggt atgttgtacg tttctttgaa
     2041 ggaccgggcc tcaaacgcgg tgttcgtggc cgctatgcct atcattctta tcttctcctt
     2101 caggtccgcg cgcgccttta tcttctcgta cacctcgtgg accaggggag catggacctg
     2161 gcagatcgga cagtaaaaac tgaagatctc gatgatcacg atctggctct tgatatcgcc
     2221 gaccttgaac gtgcctttgt ctgaaagacc caggtattcc ctttcggctc gatcctgggg
     2281 aatgggcagt tcgaaagcgg gcaagggcat ccgtctgtcc gagtagtcca actgcgtggt
     2341 ctgggccaaa gcggccgttc ctcccggaaa gagctggaaa caggcataca acgctgctgc
     2401 cgcgcacacc agaacgatca gcttcttgat gctcataccg tcctccctgg ttatcgacat
     2461 ggaacgtgaa ggcaaagagg cacaggggac catccgtctc ggactcatgg agcccgggag
     2521 gcctccctgt gttcacctct ctgcggtcgt cgggactcgg aaagtcttgt gtcctactgt
     2581 cccagtttct ttagctcgtt ctccgcttct accttcagcg cccggccctg ttttatctgc
     2641 aggtcgcaac gttcgatggt tttcaaggcg tccgcgatca tcttgtccgc cgctttctcc
     2701 atggtcaccc atggatactg aggccggtct gtcgaggtcc cgccggccgc catcgccctg
     2761 gcgcgtcctt cctgggccct cttcttcatc tcctgtgccc tggcgatctc ctc
//
LOCUS       DAOQTR010000298         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_42267_length_2812_cov_3.317737, whole genome shotgun sequence.
ACCESSION   DAOQTR010000298 DAOQTR010000000
VERSION     DAOQTR010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42267_length_2812_cov_3.317737"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1152
                     /locus_tag="PKN85_07610"
     CDS             172..1152
                     /locus_tag="PKN85_07610"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNT44314.1"
                     /translation="MERSLLIAISLLMLLPCLSRASSDPVRFFRMGDGKISIRNEHSG
                     REATVNLFAADGKLNENALDAIDRVFGFTDGRKGEHISLRLLVLLDHFTDRVAPGKTI
                     HLVSGYRSPAYNQKLKKSGGNVARTSTHMDGMALDFYLDGINGKLLWEIIRRGNCCGV
                     GHYGGKNIHLDSGKPRFWEAATSKTGTRESEFNRKLYFSTEYDRYRQGEKVRFFLTSL
                     SDFPFGVRKTASLLRDGDGENGMTALMIEGRDGEECVMINDRNASRSIRGTLPAGLEP
                     GRYRIRFDFCKRPFEQMPTTTSSNEIEIVKDWKTVQEFQGSRVQEEKRPR"
     gene            complement(1417..>2812)
                     /locus_tag="PKN85_07615"
     CDS             complement(1417..>2812)
                     /locus_tag="PKN85_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNT44315.1"
                     /translation="GGSAGKEGPIAQIGSGFGSILASYLKLSERERRIMLLAGAAGGI
                     GAIFKAPLGAALFAAEVLYSSADFEFEAIIPCILSSVIGFMVFTFYDGTATIFRIPAF
                     TLATAGQLPFYVGLGLLCALVGYFYVKVFYGTRDRFFRRLDMPKWLKPALGGLMLGVL
                     AFFLPEILAGGYQWIQSAIDGHLAVGLMIALVFGKIVATSLTISSGGSGGVFAPSLFI
                     GSMLGGFYGDICGRIFPEIVTQPAAFVLVGMGGFFAGVAKTPIAALIMVAEMTGGYSL
                     ILPMMIVSALAYLLLGKTTLYEKQVATRVDSPAHVGEYSVDIMDHLLVKDAVVPDRKI
                     ETIPEEMGFELMVDLMADSYQQDFPVVDRNGNLVGIISMTDLRTAMADSSLHRLLVAR
                     DIAVTGVTAVTPEDSLNTALQIMADMNVRELPVVERGDPGRIVSMVSRRDITRAYHNE
                     MERRGSRRTGGAKG"
BASE COUNT          626 a          859 c          795 g          532 t
ORIGIN      
        1 tgtcaacgca atccgcacac cggccgaagg catccggtgc cctttttgtc ttttactctt
       61 gaacctttga actcttgaac cctggaacag tctttgggtt gcatatcctt tgaccaccta
      121 ttataattct gcatgatttc gtccgacata tcagactcac gcctttcact catggaacgg
      181 tctttgttga tcgccatttc actgctcatg ctcctcccct gcctgagcag ggcatcgtcc
      241 gaccctgtcc gtttcttccg gatgggggac gggaagataa gcatacggaa cgagcacagc
      301 ggacgggaag caacagtgaa cctctttgct gccgatggca agctcaacga gaacgcgctc
      361 gacgccatcg acagggtctt cggttttacc gacggtcgga agggggaaca catctccctg
      421 aggcttctcg ttctgctcga tcatttcacc gacagggtcg ccccgggcaa gaccatccac
      481 ctcgtctcgg gctacagaag ccccgcatat aaccagaagc tgaagaagtc cggcggcaac
      541 gtggcgagga caagcaccca catggacggc atggcgctgg acttctacct tgacgggata
      601 aacggcaagc tcctgtggga gatcatccgg agggggaatt gctgcggcgt cggccattac
      661 gggggcaaga acatacacct cgattccgga aaaccccgct tctgggaagc ggcaacgtca
      721 aagaccggca ccagggaaag cgaattcaac cgcaagctct atttctcgac cgaatacgac
      781 cgctacagac agggggaaaa ggtccgtttc tttctgacgt ccctgagcga cttccccttc
      841 ggggtccgca agaccgcctc attgctcagg gacggcgacg gagagaacgg catgacggcg
      901 ctgatgatcg aagggcggga cggggaagag tgcgtcatga taaacgaccg gaacgcgtcc
      961 cgatcgatcc gcggaacgct ccccgcggga ctggaaccgg gccgatacag gatccggttc
     1021 gatttctgca agagaccctt tgaacagatg ccaacgacaa cctcatcgaa cgagatagag
     1081 atcgtcaaag actggaagac ggttcaagag ttccagggtt caagagttca agaagaaaag
     1141 aggccccggt aagatcgtcc agcgttccgt tccggttgac ggggcggtcg ggtcggaata
     1201 cgcgtctctg actctggaac tcttgaactt tttttgccgg gtgtgatccg aagcgtggcc
     1261 gcccggggaa gaagcagcgt gtcccccccc gcgttacccc tcagcccgct ttatcgacct
     1321 tcatctttct gccagttcac ctgccccgca tcgagaagga cagacgacag acccctcaat
     1381 tcccacggct tccgtccgct tcgatcgtgt cggggcctag cccttcgccc ctcccgtcct
     1441 gcgggagcct ctgcgttcca tctcattgtg gtatgcgcgg gtgatatccc ttctgcttac
     1501 cattgacacg atcctgcccg ggtcccccct ctccaccacc ggcagttccc gcacgttcat
     1561 atcagccatg atttgcaagg ccgtgttcag ggagtcctcc ggggtgaccg ccgtcacgcc
     1621 cgtaaccgct atgtcccgcg cgacaaggag gcgatggagc gaagagtccg ccatggcggt
     1681 gcgaagatcg gtcatggaga tgatcccgac aaggttgccg ttcctgtcca caaccggaaa
     1741 atcctgctga tatgaatccg ccatgaggtc caccatgagc tcgaatccca tctcttcggg
     1801 gatggtctcg atcttcctgt cgggcaccac cgcgtccttg accaggaggt ggtccatgat
     1861 gtccaccgag tattccccca cgtgagcggg agaatcgacc cgcgtggcca cctgcttttc
     1921 atagagggtc gtcttcccga gaagaaggta cgcaagggcg gacacgatca tcatgggaag
     1981 gatgaggctg tagcctccgg tcatctcggc aaccatgatg agcgcggcga tcggggtttt
     2041 tgccaccccg gcgaagaaac ctcccatgcc gacgaggaca aaggcggcgg gctgggtcac
     2101 gatctccgga aatatccttc cacagatgtc cccgtagaaa cccccgagca tggaaccgat
     2161 gaaaagggac ggggcgaaca cacccccgct cccgccggag ctgatggtca gcgacgtggc
     2221 cacgatcttg ccgaaaacga gggctatcat gagacctacc gcaaggtgcc cgtcgatggc
     2281 cgactggatc cattggtacc ctcccgcgag gatctcgggc aggaagaagg cgaggacacc
     2341 gagcatcaga cctcccaggg cgggcttcag ccacttcggc atgtcaaggc gcctgaagaa
     2401 ccggtccctc gtaccgtaga aaactttaac atagaaatag cccacaagag cgcagagaag
     2461 tcccagcccg acatagaacg gcaactgtcc ggcagttgca agagtgaatg cggggatccg
     2521 aaagatcgtc gccgtcccgt catagaacgt gaagaccatg aatccgatga cggaagaaag
     2581 gatacagggg atgatcgcct cgaattcgaa atcggcgctg ctgtaaagga cctcggcggc
     2641 aaagagcgcg gcacccaaag gagccttgaa tatggcccct attccgcccg cggccccggc
     2701 aaggagcatg atgcgccgct ccctttcgct cagcttcaga tacgacgcga gtatggagcc
     2761 gaaccccgag ccgatctgag ctatgggccc ttccttgccc gccgagcccc cc
//
LOCUS       DAOQTR010000299         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_42297_length_2811_cov_3.117199, whole genome shotgun sequence.
ACCESSION   DAOQTR010000299 DAOQTR010000000
VERSION     DAOQTR010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42297_length_2811_cov_3.117199"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..2280)
                     /gene="glgX"
                     /locus_tag="PKN85_07620"
     CDS             complement(211..2280)
                     /gene="glgX"
                     /locus_tag="PKN85_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNT44316.1"
                     /translation="MIDRSDSTCFPLGPTVYGNGVNFSVFSKNADAVELLLFDHVDDG
                     TPARVIRLDPEEDRTYHYWHVFVPGLVPGQVYGWRVRGPSAPERGHRFDAGKVLLDPY
                     GKAVAVPHGYSRMAASQPGDNTAVAMKSVVADLDDYDWEGDGLLMRPFARTVIYEAHV
                     AGLTKNPNSGVAPEKRGTYAGLIEKIPYLKDLGITAIELMPVFQFDDQDAPPGKTNYW
                     GYAPVSFFAPHSGYSSRKDNLGPLDEFRDMVKALHKAGIEVILDVVYNHTAEGDHRGP
                     TISFRGFGNDIYYVLDNDRARFANYTGTGNTLNANQPIVRRMIIDSLHFWVDKMHVDG
                     FRFDLASILSRDEFGRPMENPPVLWDIESDPILAGVKLIAEAWDAAGLYQVGRFIGDN
                     WKEWNGKFRDDVRAFLRGEEGTVTRLAQRLMGSPDIYGGREREPEQSVNFVTCHDGFS
                     LNDLVSYNHKHNETNGEENRDGTDYNLSWNCGFEGPTDDPAIDGLRNRQIKNFFTITL
                     ISVGTPMLSMGDEVRLTRYGNNNSYCQDNETSWFDWSLLTRHADMLRFTKSLIALRLS
                     RRYAKAERSMTLNELLRKARMSWHGVKLGEPDWADHSHSLAVTLEVEKGLLFHIMLSS
                     YWGPLEFELPPTGSARGSWRRLIDTSLESPDDFCAWNLAPRIAGDEYQLQSYSVAILF
                     AHATEEV"
     gene            2514..>2811
                     /locus_tag="PKN85_07625"
     CDS             2514..>2811
                     /locus_tag="PKN85_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HNT44317.1"
                     /translation="MKKSYTILIATILAVTVLCVALDAEAVPNPGAIQWRASPRAFVT
                     SLYVGVLGRQPESQAVVNGWATQVNATPHSRYQVFWRFVNSPEYQGSRWARQKRE"
BASE COUNT          526 a          821 c          876 g          588 t
ORIGIN      
        1 caacgcgggc ctcaccatca cagacttcat ggacgaagaa ggcttctact atatctcttc
       61 caccttatcc ggctccgcgt tctgacatat gtcatcggca acgggcggac gggacggagt
      121 gtctgcatca ttttggtcat tggtcattgt ctgtttatct ggtcattgga atttggtcaa
      181 ttggtcatta tcctcgacag ccgtgatcca tcacacctct tctgtcgcat gagcgaagag
      241 gatcgccacg gaataagatt gcagctggta ttcgtctccc gctatccgag gggccaggtt
      301 ccaggcgcag aagtcgtcgg gggactcgag ggacgtgtcg atcagtcggc gccaggatcc
      361 ccgggctgac ccggtcggcg gcagctcgaa ctcgagaggc ccccagtagc tgctgagcat
      421 tatgtggaac aggaggcctt tctcgacctc gagggtcacc gcgaggctgt gcgagtggtc
      481 ggcccagtcg ggctcgccga gcttcacccc gtgccacgac atgcgggcct tgcgaaggag
      541 ttcgttgagg gtcatcgacc gctcggcctt tgcgtaccgc cgggaaagcc gaagcgcgat
      601 gaggctcttg gtgaagcgga gcatgtccgc gtggcgggtg agaagagacc agtcgaacca
      661 gctcgtctcg ttgtcctggc agtagctgtt gttgttcccg taccgggtga gccgcacctc
      721 gtcgcccatg gagagcatcg gcgtgcccac ggagataagg gtgatggtga agaagttctt
      781 gatctggcgg ttccggagcc catcgatggc agggtcgtca gtggggccct cgaaaccgca
      841 gttccagctc aggttgtagt ccgtgccgtc cctgttttct tctccgttcg tttcgttgtg
      901 cttgtggtta taggagacga ggtcgttgag ggaaaacccg tcatggcagg tcacgaagtt
      961 cacgctctgt tcgggctcgc gctcccgccc gccgtagata tcggggcttc ccatcaggcg
     1021 ctgggcaagc cgtgtcacgg tcccctcttc gcccctgaga aaggcccgca cgtcatcccg
     1081 gaacttgccg ttccattcct tccaattgtc gccgataaag cggccgacct ggtacagccc
     1141 cgccgcgtcc cacgcctcgg cgatgagctt gaccccggcg aggatgggat cggactcgat
     1201 atcccacagc acgggcgggt tctccatggg ccggccgaac tcgtcgcggg agaggatgga
     1261 ggccaggtcg aaacggaaac cgtccacgtg cattttgtcg acccagaaat ggaggctgtc
     1321 gatgatcatc ctgcggacga tgggctggtt ggcgttcagg gtgttgcccg tccctgtgta
     1381 gttcgcgaat cgcgccctgt cgttgtcgag gacgtagtag atgtcgttcc caaagccccg
     1441 gaaggagatg gtcgggcccc tgtggtctcc ctcggccgtg tgattgtaga caacatcgag
     1501 gatgacctcg atgccggcct tgtggagggc ctttaccatg tcgcggaact cgtcgagggg
     1561 accgaggttg tctttccggg aactgtaccc gctgtgggga gcgaagaaag acacgggggc
     1621 atacccccag tagttcgtct tgcccggggg agcatcctgg tcgtcaaact ggaatacggg
     1681 catgagttcg atggccgtga tgccgaggtc cttcagatag ggtatcttct ctatcagccc
     1741 cgcgtaggtg cctctcttct ccggagcaac accggaattg ggattcttcg tcagtcccgc
     1801 cacgtgcgcc tcgtagatca ccgttctggc gaaggggcgc atgaggagcc cgtccccctc
     1861 ccagtcgtag tcatcgagat cggccacaac gcttttcatc gcaaccgccg tattgtcgcc
     1921 cggctgggag gcggccatgc ggctgtatcc gtgagggacg gccacagcct tcccgtaggg
     1981 gtcgagaagg accttgccgg catcgaacct atggcccctt tccggcgcgg aggggccgcg
     2041 gacgcgccag ccgtagacct gccccggcac gagaccggga acgaagacgt gccagtagtg
     2101 gtaggtacgg tcctcttcgg ggtcgagcct gatcacgcgc gccggggttc cgtcgtcgac
     2161 gtgatcgaag agaagcaact ccaccgcgtc ggcattcttt gaaaagacgc tgaagttcac
     2221 cccgttaccg tacacggtgg ggccaagagg aaaacaggtg ctgtcggagc ggtcgatcat
     2281 ttcaccagga cagtctctat gctagcacct attcagaccg tcgtcaaagg aaagcgagcg
     2341 ccccgccctt gacattgcct ttcccggcgg gtatcctgtt ttaagccagc ctacccggag
     2401 tcgtccacaa cgaggaacgc tacagaaact gatgatgcag gtatgccgta cctggtttcc
     2461 atgaaatgga tggtggaagg ctcccatatt tcgatcattg tggaggaggg caaatgaaga
     2521 aaagctatac catactcatc gcaaccatac ttgccgtcac tgttctttgc gtcgctctcg
     2581 atgccgaagc ggtcccgaac ccgggggcca tacagtggag ggcatccccg cgcgcctttg
     2641 tgacatccct ctatgtcggg gtccttggca ggcagcccga aagtcaggct gttgtgaacg
     2701 ggtgggccac gcaggtgaac gccacccccc actcccgcta ccaggtattc tggaggttcg
     2761 tcaattcgcc cgaataccag ggaagccggt gggcgcggca gaaaagggaa t
//
LOCUS       DAOQTR010000300         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_42517_length_2802_cov_3.202039, whole genome shotgun sequence.
ACCESSION   DAOQTR010000300 DAOQTR010000000
VERSION     DAOQTR010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42517_length_2802_cov_3.202039"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /locus_tag="PKN85_07630"
     CDS             <1..1720
                     /locus_tag="PKN85_07630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT44318.1"
                     /translation="CWQSCAGRSPPLGRGWGEARGEHAPLSAAAVVEEVAQADRDRAE
                     GDLLPRDFVFREQAHFEGLLARAEVEPVELSEALCDLSRRSKKPLIAVWMAGVVAEKG
                     RDLLIENNIPVYRTPEEAVKAYLYMHQYRKNIEMLYEIPEDISPGHSRLNNHQKAIIR
                     NCLREGTNSLSMEQSVSLMKTYDIPFPPTSFPRGMDEAVREAEAMGFPVTLKVAGGES
                     AGPGELFPGIASRQTLLEVLGGIRERSSRRPDPTDMPEMMIQKTVRGKTAGWFLNTRR
                     DIDFGSTIFLSAAREDGPSGDRSVVALPPLNQTLAKIFLEDAGVQEALRKEDRQGLQS
                     LLRQFEALCVNFACLIVDFPEILTIEAGPIVVDDGGVYMTDAVIDLDGGFSRQPARYY
                     SHLVITPYPTQYVTPWTLRDGTDIVMRPIRPEDEPLSREMLARTSEEALRVRFFSSVN
                     VQEIPHSTLMKFCNVDYDREVALVAEVREAEEKRIVGGGRLIIEPDLKRAQFAVLVHD
                     GLHGKGLGEKFVDMLIGIAQERGLEGIYGIVLTENEKMLRLCKKMGFVSKRLPDGITR
                     VEMKLN"
     gene            complement(1724..>2802)
                     /locus_tag="PKN85_07635"
     CDS             complement(1724..>2802)
                     /locus_tag="PKN85_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing transglutaminase
                     family protein"
                     /protein_id="HNT44319.1"
                     /translation="IELRRVRKNDDHTFTMPRFIERRIRYTALSALSNVIPEENISLK
                     THLQLPPDLSPRIVSLAKSLAVRGDRNATVNRILSHLASDRYGYSLKDLPVSKNPLES
                     FLFDSPKGNCEYFASAMAVLLRVNEIPSRLVGGYQGGYYNEMGNYYIVPQKNAHVWVE
                     AHIPGKGWVRFDPTPAGAGLSSLSGRGALSKLRMFLDTINYYWYGIILTYNLERQISI
                     TRTIVSGLRRPSSFSFSPLMKKTAQYSALFAAAIGLILLAWFLISSAKRREMVMLDRF
                     LKRLKTAGYEKQTSEGLEEFIRRIGDDGLRTASMEFARAFEEIYYGDRRFTADDLKRL
                     NHLAGKIDDVAGQEDRMADLTPGR"
BASE COUNT          581 a          780 c          869 g          572 t
ORIGIN      
        1 ctgctggcag tcgtgcgcgg gccgctcacc gccactcggg cgggggtggg gcgaggctcg
       61 cggtgagcac gccccactgt ccgctgcggc ggtcgtcgaa gaagtggcgc aggctgatcg
      121 cgatcgagcg gaaggcgatc tgctcccacg ggatttcgtc ttccgtgaac aagcccactt
      181 cgagggactc ctcgcccgcg cggaagtcga gcccgtggaa ctttcggaag ctttgtgcga
      241 cctgtcccgc cggtccaaaa agccattgat cgcagtatgg atggcgggcg tcgtggctga
      301 aaagggaagg gacctgctca tcgagaacaa catacccgtt taccggaccc ccgaagaggc
      361 ggtcaaggcc tatctataca tgcaccagta ccggaagaac atagaaatgc tctacgagat
      421 acccgaagac atctcgcccg gccattcacg gttgaacaac caccagaagg ccattatccg
      481 gaactgtctc agggaaggga ccaactccct ttccatggag cagtccgttt ctctgatgaa
      541 gacctatgat attccttttc cgccgacgtc attccccagg ggtatggacg aagctgttcg
      601 cgaggcggaa gcgatgggtt ttcccgtaac gctcaaggtc gcgggcgggg aaagcgcggg
      661 accgggcgaa ttgtttcccg gtattgcttc ccggcagacc cttctcgagg tcctgggcgg
      721 gatcagggag agatcgagcc gaagaccgga cccgacggac atgccggaaa tgatgatcca
      781 gaaaacggtt cgtgggaaga cggcgggatg gttcctgaac accaggaggg acattgactt
      841 cgggtccacc atctttctct cagccgctcg cgaggatggt ccgtccggcg ataggtccgt
      901 cgtggccttg cctcccctga accagacgct tgcaaagata ttcctggagg acgcgggagt
      961 gcaggaagcc cttcggaaag aggaccggca agggctccag tcgctgttga gacaattcga
     1021 ggccctatgt gtcaatttcg cgtgcctcat cgtcgatttt ccggagatcc tcacgatcga
     1081 ggccggtccc atcgtggtcg atgacggcgg cgtctatatg actgacgcag tgatcgatct
     1141 tgacggcggg ttctcgaggc aacctgcgcg ctactattcc catctcgtca taacccccta
     1201 tcccacccag tacgtgacgc cctggacatt gagggacgga acggacatcg tgatgcgccc
     1261 aatacgcccc gaggacgagc ctctgagtcg tgagatgctt gcccggacct ccgaggaggc
     1321 cttgagggtg cggttcttct cttccgtcaa cgttcaggaa ataccgcaca gcacccttat
     1381 gaagttctgc aatgtcgatt acgaccggga ggttgccctg gtggcggagg tcagggaggc
     1441 cgaggaaaag cgcattgtcg gaggcggacg gctcatcata gagcccgatc tcaagagggc
     1501 gcaattcgcg gtcctcgtcc atgacggtct ccacggcaaa ggtctgggcg agaagttcgt
     1561 cgatatgctc atagggatcg cccaggagag ggggcttgag ggcatttacg ggatagttct
     1621 caccgagaac gagaagatgc tccggctctg caagaaaatg ggctttgtat caaagcggct
     1681 gcccgatggg atcacgcggg tggagatgaa attgaattga cccttaccgc cccggcgtca
     1741 gatcggccat cctgtcctcc tgtcctgcaa catcgtcgat cttgccggca agatggttca
     1801 gtctcttgag atcatccgcc gtgaaccgcc tgtccccgta atagatctcc tcgaaagccc
     1861 gggcaaattc catggaggcc gtcctgaggc cgtcatcgcc gatgcgccgg atgaattcct
     1921 cgaggccctc cgatgtctgt ttttcgtatc ccgctgtctt gagccgcttg aggaaacggt
     1981 caagcatcac catctccctg cgcttcgccg aggagatgag gaaccaggcc aggagaatga
     2041 ggccgatggc tgccgcaaag agcgccgaat attgcgccgt cttcttcatg aggggagaaa
     2101 aggagaagga cgacggcctt ctaaggcccg acacgatggt cctcgttatg gatatctgcc
     2161 tttcaaggtt gtaggtgagg atgatcccgt accagtagta gtttatggtg tcgaggaaca
     2221 tccggagctt cgacagggcc cccctccccg agagggacga aagccccgcc ccggctggtg
     2281 tcggatcgaa tcggacccac ccctttccgg gtatgtgtgc ctccacccac acgtgggcat
     2341 tcttctgggg cacaatatag taattgccca tttcattgta gtatccgccc tgataccctc
     2401 caacaaggcg ggaagggatc tcgttgacac gcaggagaac ggccattgcc gaagcgaagt
     2461 actcgcagtt tccctttggg gaatcgaaga gaaagctttc gagagggttc ttcgagacgg
     2521 gaaggtcttt gagggaatag ccgtacctgt cggaggcgag gtggcttagg atgcgattca
     2581 cggtggcgtt cctgtcaccc cgaacggcaa ggctcttcgc gagggagacg atgcgcggcg
     2641 agaggtcggg gggaagctgg aggtgggtct tcagggagat gttctcctcg ggtatgacgt
     2701 tcgagagagc cgacagggcg gtataacgga tccgcctctc tatgaagcgc ggcatggtga
     2761 atgtgtggtc gtcgttcttc cttacccttc tgagctcgat gt
//
LOCUS       DAOQTR010000301         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_42713_length_2793_cov_4.095690, whole genome shotgun sequence.
ACCESSION   DAOQTR010000301 DAOQTR010000000
VERSION     DAOQTR010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42713_length_2793_cov_4.095690"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1801
                     /locus_tag="PKN85_07640"
     CDS             242..1801
                     /locus_tag="PKN85_07640"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44320.1"
                     /translation="MEQTDQTLTDFLLDPRNYPERTRAVSHVETHISHVFIGDRFVYK
                     VKKAVNFGFLDFSTLKKRRFFCEQEVVLNSRLAKDIYLGVQPFYRSHAGFSFARKGRG
                     LPVEYAVRMKRIPLERLLFKLIGEGRPLYGSLEDVGRTLARFHEDIVPYRGRGFGGVE
                     SIGAATEENFEQIRPFTALTVGRKTLASIARYTRAFIVDHRARFAERRRKGFIREGHG
                     DLHCQHVCLEHPPVIFDCIEFNRGFRVIDILEDIAFLFMDLEYRGRFDLSARLHKAYF
                     AHQPKALDEELLRFYKVYRAVVRGKVEGFAARGVEDRSQREGAVARARDYFGLADHYI
                     NHFQKPFNPVVFMGLSGSGKSTIARDFSPNAVILRSDEMRKEITGVRKGRHAYSSFGE
                     GIYTPELTNRIYRLMLDRATEHAGMGKKVIVDATYLRAEQRRDFHETCIERGLNPFFI
                     HCFASEAVLKERIRKRMEAGTDISDADIAVLDHQLEHLEDPEELPRYRTLRVNTEDAI
                     HNIVHALREFL"
     gene            1896..2069
                     /locus_tag="PKN85_07645"
     CDS             1896..2069
                     /locus_tag="PKN85_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44321.1"
                     /translation="MMFGVEYECRDCGRAFRVDEEEKREPRCPGCEGGNVTIRQARPL
                     PQWIMRKKEAAPT"
     gene            2129..2470
                     /locus_tag="PKN85_07650"
     CDS             2129..2470
                     /locus_tag="PKN85_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44322.1"
                     /translation="MEIRIRRVGSRTLTGNVSGRVNTYRVDQDGREFLLTRTSNTLAS
                     NIGIAGQRGILYVETEDNKVHHQVVAPSGACGLNTDDEVVEGLSPLALRAVLMVDKCD
                     EPGEITITREE"
     gene            2557..>2793
                     /locus_tag="PKN85_07655"
     CDS             2557..>2793
                     /locus_tag="PKN85_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNT44323.1"
                     /translation="MDGKDYYEVLEISVEATDRQVKEAYRRLAFQYHPDRNGSDPGAV
                     ERMKSINEAYAVLSDPAKRQRYDNLRQSYGTSAYD"
BASE COUNT          641 a          739 c          898 g          515 t
ORIGIN      
        1 tcatgggata ccccgagtgg aagggacttc tgtgctcgat acggaaggcg tgatagagga
       61 agaagtcgca cagcggaagg ctgtctgcat ccttccccgg cgggacccgt aaagatcaac
      121 ttgaaggccg gcttcacgag ccggcctttt tttcgtgccc gggatcttgt cgggccgccg
      181 tggcagggcc ttgctttcgc ggctaataga tactagcata gagtatagcc gagagcgtca
      241 catggaacag acggatcaga cactcaccga ttttctcctt gatccccgaa actatcccga
      301 gagaacgcgt gccgtctcgc acgtggagac gcacatatca cacgtgttca tcggtgacag
      361 gtttgtttac aaagtgaaga aagcggtgaa tttcggtttt ctcgatttct cgacgttgaa
      421 gaagcgccga ttcttctgcg aacaggaggt ggtgctcaat tcccgtcttg caaaggacat
      481 ttatctcggc gttcagccct tttacaggag ccatgccggg ttcagttttg cccggaaggg
      541 acggggtctg cctgtggaat acgcggtaag aatgaagcgc atccccctgg agcgcctgct
      601 tttcaagctg atcggcgaag ggcggcccct gtatggctcg ctggaagacg tgggcaggac
      661 gctggcccgc ttccacgagg acatcgtgcc gtacaggggg cgcgggttcg gaggtgtcga
      721 atccataggc gccgccacgg aggagaattt cgagcagata aggcccttca cggcactgac
      781 cgtcggcagg aagaccctcg cttcgatcgc gcggtatacc cgcgcgttca tagtcgacca
      841 cagggccagg tttgctgaga gaaggaggaa ggggttcatc cgggaaggcc acggggacct
      901 ccactgtcag catgtctgtc tggagcatcc tcccgtcata ttcgactgca tcgagttcaa
      961 caggggtttc agggtcatcg atatcctgga ggacatcgct tttctcttca tggacctcga
     1021 ataccgggga aggttcgacc tgtcggcgag gctccacaag gcctatttcg cacaccagcc
     1081 gaaggccctc gatgaggaac tgctccggtt ctacaaggtg taccgcgccg tcgtgcgggg
     1141 caaggtggag ggattcgccg ccaggggcgt ggaagacagg tcgcaaaggg aaggggctgt
     1201 ggcgagggcc cgggactact tcggccttgc cgatcattac atcaaccact tccagaagcc
     1261 attcaatccg gtggtcttca tgggtctgtc gggatcgggc aaatcgacga tcgcgaggga
     1321 tttctcaccc aacgccgtga tcctcaggtc ggacgagatg aggaaggaga taacgggcgt
     1381 gaggaagggc aggcacgcgt acagttcctt tggagaaggg atctacactc ccgagctcac
     1441 caaccggatc taccgcctga tgctggacag ggcgaccgag cacgccggga tgggaaagaa
     1501 ggtgatcgtc gacgcgacct acctcagggc ggagcaaaga cgggatttcc atgagacctg
     1561 tatcgagagg gggcttaatc ctttcttcat tcactgcttc gccagcgagg ccgtcctgaa
     1621 ggagaggatc agaaagagga tggaggcggg tacggatatc tccgacgcgg acatcgccgt
     1681 gctcgatcac cagctcgaac accttgaaga cccggaagag cttcctcgct accgcactct
     1741 ccgcgtcaac acggaggacg ccatccacaa catagtccac gcgctgagag agttcctgta
     1801 ggaacagcgc ctccccgggg gtgaaggccg ggggtctgga aaaagggccg cggaagtact
     1861 atactcacat aagctccgct tcatggaggt gacatatgat gtttggcgtc gagtacgaat
     1921 gcagggactg cggccgggcc ttccgggtcg atgaagagga gaaaagggag ccgaggtgcc
     1981 cgggctgcga aggcgggaat gtgacgataa gacaggcccg tccgctgccg cagtggatca
     2041 tgcgcaagaa agaggccgcg ccgacctgaa agtagaaaag agggtggcgt tgccattcgt
     2101 gaagatcttt gatcaggacg cgtctcgcat ggaaataagg atcaggagag tgggttcgag
     2161 aaccctgacg ggcaacgtgt cgggaagggt gaacacatac agggtcgatc aggacggcag
     2221 ggaatttctg cttacccgga cctccaacac gcttgcgagc aatatcggca ttgccgggca
     2281 gaggggcatt ctctacgttg agaccgagga taataaggtc caccatcaag tggtggcccc
     2341 gagtggggcc tgcggactca acaccgatga cgaggtggtt gaagggctct cgcccctcgc
     2401 gcttcgcgcg gtgctcatgg tcgacaagtg cgatgagccg ggcgagatca cgataacaag
     2461 ggaagaataa cagggcgttc cgatcggaca cggggaaaca gcacctgccc tcgagtcccc
     2521 gatcggggca tggcaggccc tgcagaggga aggaatatgg acggaaaaga ctattacgag
     2581 gtcctcgaaa taagcgtcga ggccacggac aggcaggtca aggaggcata tcgcaggctt
     2641 gcgttccagt atcacccgga cagaaacggg tcggacccgg gtgccgtgga gcggatgaag
     2701 agcataaacg aagcgtatgc cgttctgtcc gacccggcca agagacagcg gtatgacaat
     2761 ctgaggcaaa gctacggcac ctccgcctac gac
//
LOCUS       DAOQTR010000302         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_42755_length_2791_cov_4.311404, whole genome shotgun sequence.
ACCESSION   DAOQTR010000302 DAOQTR010000000
VERSION     DAOQTR010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42755_length_2791_cov_4.311404"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKN85_07660"
     CDS             <1..432
                     /locus_tag="PKN85_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44324.1"
                     /translation="FAVIRDPVLRKTWLKGSEGLIPHVNVLGITAALAAYRDGEEWLT
                     QCLAYLESNRGLLEHFLGERSPSLSMSRMEATYLAWIDCRESRIPGNPFRFFLEEGRV
                     ALNDGREYGKGGEGFVRLNFACPRGSLTEALERMADAMQRL"
     gene            complement(461..1243)
                     /locus_tag="PKN85_07665"
     CDS             complement(461..1243)
                     /locus_tag="PKN85_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44325.1"
                     /translation="MYDTKSFNPEADRLPPMYTGHNPELLYNGIEMRKQRAMRGTGET
                     EEQHRARMAREIALPLMGSLDFDSVYAVRVTPAEVRRDGKDGLIHVSCRLCAAFEAGR
                     EDPGKKAFVVRYLPHLDNRYTIARKDGTRVTIEEIKFSEYAVVIANARDLPIQRTVGP
                     QAREDGRRQKEGDEKPEGERIEEAIELTADQARRMEGGIMALLIGKLAPPYTSHEEIG
                     RKPTAERPGTYLGRYHYLYMDLMTIWFYDVTTGKVLKKMRFS"
     gene            complement(1740..>2791)
                     /locus_tag="PKN85_07670"
     CDS             complement(1740..>2791)
                     /locus_tag="PKN85_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT44326.1"
                     /translation="MKPIIFLTPVLQLIVFGYAVTMDVTNIRMAVHDLDKTSVTREIA
                     RRFEASGYFRIVRFAASEQEVRDLLDRGKATVVLNFDRGFTSDLEARRGASVQILIDG
                     ADSNTALVAAGYAGAIISRYGRDIATRMASWTPGSLDVRPRAWYNADLRSRNYNVPGV
                     IAIMVLLVCLLLTSMAIVREREIGTMEQLMVTPIRPAELILGKTIPFALIGFFDMFLV
                     TVVGVLWFDVPIRGSIPLLVLATAVYLLSVLGFGLFISTISRTQQQAMMATFLFFAPA
                     ILLSGLMFPIENMPRAVQFITYLNPLRYFLVIIRGIFLKGSGMAILWPQFLALFLLGA
                     SVVATSSLRFKKRLG"
BASE COUNT          537 a          818 c          873 g          563 t
ORIGIN      
        1 ttcgcggtga tcagggaccc ggtgttgagg aagacctggc tgaagggcag cgaaggtctt
       61 atcccccacg tgaatgtcct ggggatcacc gcggccctgg cagcctacag ggacggcgag
      121 gagtggctca cccaatgtct tgcctatctc gagagcaaca ggggtcttct ggagcacttc
      181 ctgggcgaga gatcgccttc cctttcgatg agccgcatgg aggcgaccta tcttgcctgg
      241 atcgattgca gggagtcgcg gataccgggg aacccgtttc gcttctttct cgaggagggg
      301 agggtggccc tgaacgacgg ccgggaatac gggaagggcg gagaggggtt tgtcaggctc
      361 aactttgcct gtccgagagg gagcctcacc gaggcgctcg agcgcatggc cgatgccatg
      421 caaaggttgt agtcgccgaa agaaggggcg gtcggaacgg ctaagagaac cgcatcttct
      481 tgaggacctt ccctgttgtc acgtcgtaga accatattgt catgaggtcc atgtacaggt
      541 aatgatatct cccaagatag gtgcctggtc tttccgccgt cggcttgcgg ccgatctcct
      601 cgtgcgaggt gtacggcggc gcgagcttgc cgatgagaag cgccatgata ccgccctcca
      661 tgcgtcgtgc ctggtcggcc gtcaactcta tcgcctcttc gatcctttcg ccctccggct
      721 tttcgtcgcc ctccttctgt ctcctgccgt cctcgcgtgc ctgcggccct acggtcctct
      781 gtatcgggag gtccctcgcg ttggcgatga ccaccgcgta ttcgctgaac tttatctcct
      841 cgatggtcac ccgggtgcca tccttcctgg ctatggtgta cctgttgtcg aggtggggca
      901 ggtacctgac gacgaaggct tttttcccag ggtcctcccg gcccgcctcg aacgcggcac
      961 agagccggca gctcacgtgg atgagtccgt ccttcccgtc acggcggacc tcggcggggg
     1021 tgaccctcac ggcatagacg ctgtcgaaat ccagggaacc catgagggga agggctatct
     1081 cccgggccat cctggccctg tgctgttcct ctgtctcccc cgttccccgc atcgctcttt
     1141 gttttctcat ctcaatgccg ttgtagagga gttccgggtt gtgtcccgtg tacatgggag
     1201 ggagcctgtc cgcctccggg ttgaaagact tcgtgtcgta caccgtggag agaaagccgt
     1261 ttcccggccc acccggccga tgtcccccgc cggatccccc cttgcccgac cccgaacaac
     1321 cggccaagaa aagaacgcta catgccagta atgcggcaac ggaagtgaag aacccccggc
     1381 gatatctcat attctccctt tcctcctttt gcctatcatt ctggtatcgc ctgtcgccga
     1441 ttgtcaacac caaaaggcgg ttcggccgtc gagccgacac tgtcggggaa acgccgtcca
     1501 ccccgccgga tactcttcag cttgcctctg gaatgtgcaa accctcaaca ggctgttgat
     1561 aaacccattt tctacgtcat tccggcgaag gccttaaatc cagaggtttc tctaacccct
     1621 cgtttttcct ggattccggc cttcgccgga atgacgaaag agcacgaatc gtcttcagaa
     1681 atcactttgt caacagcctg tcaagcgatt caagccattc aagccattca agccgtcttt
     1741 catcccaacc gcttcttgaa cctcagcgaa ctcgtggcga cgacggatgc cccgagaagg
     1801 aagagggcga gaaactgggg ccagaggatg gccatgccgc tgcccttcag gaagatacca
     1861 cggatgatga cgaggaaata ccgaagtgga ttgaggtagg tgatgaactg gacggcccgg
     1921 ggcatgtttt ctatggggaa catgaggccc gacaggagga tcgccggggc gaagaagagg
     1981 aacgtcgcca tcatcgcctg ctgctgggtc cgggagatgg tggagatgaa gaggccgaag
     2041 ccgaggacgg aaaggaggta cacggcagtc gcgaggacga gaaggggaat ggagccccgt
     2101 atggggacgt cgaaccagag gacgccgacg acggtgacga ggaacatgtc gaagaacccg
     2161 atgagggcga aggggatggt cttccccagg atgagttcgg cggggcggat gggcgtgacc
     2221 atgagctgtt ccatggtgcc gatctcccgt tcccggacga tggccatgga tgtgagcaaa
     2281 aggcacacga ggaggaccat gattgcgatg acccccggca cgttgtagtt cctgctcctc
     2341 aagtccgcgt tgtaccaggc gcgggggcgc acgtccaggg agcccggcgt ccatgaggcc
     2401 atccgtgtgg cgatgtcccg gccgtaccgg gagatgatgg cgccggcgta tcctgccgcg
     2461 acaagggcgg tgttcgagtc cgcgccgtcg atgagtatct ggacggaggc cccgcgacgt
     2521 gcctcgaggt ccgacgtgaa gccccggtcg aaattgagga ccactgtggc ctttccgcgg
     2581 tccaggaggt cccttacctc ctgttcggac gcggcgaagc gcacgatcct gaagtatccc
     2641 gacgcctcga aacgccgggc gatctcgcgg gtgaccgacg tcttgtcgag gtcatggacg
     2701 gccatgcgga tattggtgac atccatcgtc acggcatagc cgaagacgat gagctgaagg
     2761 acgggggtca ggaagatgat gggcttcatc t
//
LOCUS       DAOQTR010000303         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_43009_length_2780_cov_3.800000, whole genome shotgun sequence.
ACCESSION   DAOQTR010000303 DAOQTR010000000
VERSION     DAOQTR010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43009_length_2780_cov_3.800000"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKN85_07675"
     CDS             <1..721
                     /locus_tag="PKN85_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44327.1"
                     /translation="SDMMKVLRSIPRNVTLLNVLLAVALVLSGIGVVCPAMSVSDRLK
                     LPRIVPKETVLPERPAEEAAQPLPTDYAVVSEMNLFHPQRVIPIDAKKELPKPELLLC
                     GIIMHGNQRVAFVEDKKNPTTSPGRGKRQTELRKGSVYAGYSVTEINTDSIALSRGEQ
                     TVSVYLMDQAKRGGKSATSAPSEARPSSTERSSSPSPVPPAAPPSPRPSRPLPSRGPE
                     EPRSDGSKAPPSPVPPHRRDR"
     gene            complement(803..1195)
                     /locus_tag="PKN85_07680"
     CDS             complement(803..1195)
                     /locus_tag="PKN85_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44328.1"
                     /translation="MKEEQESEIRVLIVDDERLARVNLRGLLDEHPYVRVVGEARNIA
                     EARRVLDATQVDLIFLDIQMPGGSGFDLVETLEHRPHVVFVTAHDHYVTRASASQALD
                     FLLKPVDPDRLGESLQKALSYREDRSVC"
     gene            complement(1319..>2780)
                     /locus_tag="PKN85_07685"
     CDS             complement(1319..>2780)
                     /locus_tag="PKN85_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNT44329.1"
                     /translation="VVPNAIRSKPYMRPDIPYLPELATSRSAIVSYSTRSGRRLAAFL
                     KTSDPEFLFVVERPLRDVLAPIETMGKVTLATLVVMLFVITVAVFRMASPLLKKLEGC
                     MAFVRNIEAGILDGRLDAKDTDEISGLARGLNTMAQSLKESRNALEEAEHLYRGLFEN
                     AVEGIFVTDPEGIVLNANPAFASLVGYASPAEVVGRNVSLYYSTTRRNRLLDMLETHG
                     TVKNFEILFHRREGSQRRGSIYARADKDEKGKILRIQGILDDITEQREIEKERRRAED
                     AELRSVQAKLEALRYQINPHFLFNVLNSLSALARISPRQTDQLIQQLSRYLRSTISSS
                     ESGFVPLSQEMGTIESYLNIEKVRFEDDLMVNIDLPSQAMDVRVPELILQPIVENAVK
                     YGTKTSELPLKITIEGSVTDDRLLLLKVTNTGHWVSTDDGGRTGKGVGIENLRKRLTL
                     IYADQYLLRTEEDSGLVRVIIGVPLETGSRQTGSQG"
BASE COUNT          566 a          809 c          795 g          610 t
ORIGIN      
        1 aagtgacatg atgaaggtgc tgcggtcaat cccgcgcaat gtgaccctcc tgaacgtcct
       61 tctggctgtc gccctggtcc tgtcaggtat cggcgttgtc tgcccggcga tgtccgtgag
      121 cgaccgcttg aagcttccgc ggatcgttcc gaaggagacg gtcctgccgg agagacctgc
      181 ggaagaggcc gctcaacccc ttcccaccga ctatgccgtc gtcagtgaaa tgaacctttt
      241 ccatcctcaa agggtcatac ctatcgatgc gaagaaggaa ctgccgaaac cggagctttt
      301 actgtgtggc ataatcatgc atgggaacca gcgtgtcgcc ttcgtggaag acaagaagaa
      361 cccgacaaca tctcccggac gggggaagag acagacggaa ctgaggaaag gctctgtcta
      421 cgcgggctat tcggtgacgg agataaacac agacagcatt gctctctcca ggggtgagca
      481 gaccgtgtcc gtgtatctta tggaccaggc gaaacgcggc ggcaagagcg ccacgtccgc
      541 tccctccgag gcgagaccat cttccacaga gcggtcatct tctccgtccc ccgttcctcc
      601 tgctgcccca ccttctcccc gcccttcaag acctttgcca tcaagggggc cagaggaacc
      661 acgctcggac ggcagcaagg ccccgccctc tcccgtgcct cctcatcgca gggaccggtg
      721 acggcacgcc atgcccaggg aagccggtcg atcgtcttac aggccgttga tacagcatta
      781 acctcactga gcaagggctt tatcaacaga ctgatcggtc ttcccggtag ctcagggctt
      841 tctggaggga ttcgcccagg cgatcggggt caacgggctt gaggaggaaa tcgagggcct
      901 gcgacgccga cgcccgcgtg acataatggt cgtgggcggt aacaaagacc acatgcggcc
      961 ggtgctccag cgtttccacg agatcgaatc cggacccgcc gggcatctgt atgtcaagga
     1021 agatgaggtc gacctgcgtt gcatccagga cccgccgggc ctccgcaatg ttcctggcct
     1081 cacccacgac gcgaacataa ggatgctcgt cgagaagccc tctaaggttt acccgggcga
     1141 ggcgttcatc gtccactatg agtactctga tctcgctttc ctgttcttcc ttcatggttg
     1201 tttcaacctc aaatttaacg cgctaccacc gccagggaaa ccattgcctg caaccccgac
     1261 tgaagcggaa cggaaggcgt agaggcgcgt tggtaagccg aaatgtcccg atacaccctc
     1321 atccttgaga ccctgtttgc ctgcttcctg tttccagcgg taccccgatg ataacacgga
     1381 ccagcccgct gtcttcctcc gtccgtaaga ggtactgatc ggcatagatg agggtcaggc
     1441 gcttgcggag gttctcgata cccactcctt ttccggtcct tcccccgtcg tctgtcgata
     1501 cccagtgccc ggtgttggtg actttgagga gaagcagacg gtcatcggtg accgagcctt
     1561 cgatggttat cttgagcggc agttccgatg tcttcgtccc gtatttgacg gcgttttcca
     1621 cgataggctg gagtatgagt tcgggaaccc gcacatccat ggcctgcgag ggaaggtcta
     1681 tgttcaccat cagatcatcc tcaaaacgga ccttctcgat gttgagataa ctctctatgg
     1741 tccccatttc ctgacttaag ggaacaaagc cggattcgct cgaggagatg gtggaacgca
     1801 gatagcggga tagttgttga atgagctgat cggtctgtcg cgggcttatt ctggcgaggg
     1861 cgcttaagga attgaggaca ttgaagagga aatgaggatt gatctgatac ctgagggcct
     1921 caagcttcgc ctgaacggac cgaagctcgg cgtcttccgc ccggcgccgc tccttttcga
     1981 tctccctttg ttccgtgatg tcgtcgagga tgccttgaat tcggagtatc tttccctttt
     2041 cgtccttgtc cgcccgggca tagatggatc ctctcctttg agatccctca cgccggtgaa
     2101 agaggatctc aaagttcttg accgttccgt gggtttcaag catatccagc agtcggttgc
     2161 gccttgtggt ggaataatag agggagacgt tcctgccaac tacctctgcc ggagaagcgt
     2221 atcccacgag ggaggcgaag gccgggttgg cgttgaggac gatgccttcc gggtcggtca
     2281 cgaatatccc ttctaccgcg ttctcgaaca gtccgcggta caaatgttcc gcctcctcca
     2341 gcgcattgcg gctctccttg aggctctgcg ccatggtgtt gagaccccgg gcaagcccgg
     2401 agatctcgtc ggtatccttt gcgtccagtc tcccgtcgag aatcccggct tcgatgttcc
     2461 ggacgaatgc catgcatccc tccagctttt tcaagagggg gctggccatc ctgaagacgg
     2521 cgacagtgat gacaaagagc atgacaacga gggttgcgag cgtaaccttg cccatcgtct
     2581 cgataggcgc gagaacatcc cgcagaggtc tttcgacaac gaacagaaat tcagggtctg
     2641 aggtcttcag aaacgcggcc aggcggcgcc ccgatctggt ggaatagctg acaatggcgc
     2701 tgcggcttgt cgccaactca ggcaggtaag ggatgtcggg acgcatgtat ggtttggacc
     2761 gtattgcgtt ggggaccacc
//
LOCUS       DAOQTR010000304         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_43191_length_2772_cov_4.433934, whole genome shotgun sequence.
ACCESSION   DAOQTR010000304 DAOQTR010000000
VERSION     DAOQTR010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43191_length_2772_cov_4.433934"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKN85_07690"
     CDS             complement(<1..461)
                     /locus_tag="PKN85_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNT44330.1"
                     /translation="MARNVMQVKEKKDRYQVCYIPGDDSGYDVMEAAMIVMQALNPPI
                     DWVRADMGWSMWEKSCKEFPEGDKRRNTVPQETIDKVRDTDATFMAAITSKAGVKGFK
                     SAILQMRQIFDLYINFRPAKLMPGLECPLKGDPKIDIKLFRENTEDLYSAV"
     gene            complement(937..1428)
                     /locus_tag="PKN85_07695"
     CDS             complement(937..1428)
                     /locus_tag="PKN85_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNT44331.1"
                     /translation="MKVTGKVWKYGDDINTDVIFPGKYTYTVSDRNEMGKYALEDLDP
                     EFTKNAQPGDIIVGGKNWGCGSSREQAVTCIKMRGIGAIIAKGFARIYYRNALNEGLP
                     IVVSPEAVDALEKGDEVTVDFDKSEIVTPKGTFKFPPYPDFVKGLIEDGGLIPHVKKS
                     LGL"
     gene            complement(1433..2689)
                     /locus_tag="PKN85_07700"
     CDS             complement(1433..2689)
                     /locus_tag="PKN85_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNT44332.1"
                     /translation="MGKTFAEKVLGKAAGEPVSAGQVVIVEPHFCMSHDNAAPIWGTF
                     EKIGVKKVWKPDHLVFILDHAIPAPTDKHAENHMKIRQVVKEQGIRYFYDVTSKGGVC
                     HQIMCEEGFALPGLIVVGSDSHTCTYGAFGTFSTGIGRSEMAATWATGQIWFRVPESM
                     KITVTGKFKKGVSAKDLILKIAGDIKADGADYMSIEFHGPAIEEMSLSERMTLCNMGI
                     EVGAKNAVCPPDQKVLDFIKPIAKTDQWEAIWADDDAVYAKELHYDLGDLVPAVAKPH
                     TVDNYAPIDEVKGTPIHQAFLGSCTNARIEDLRLAAGILKGKEVAVRTIVLPASWTVY
                     RQALEEGLIEVFLDAGCVIVNPGCGPCMGNHEGILAPGETCISTANRNFKGRMGNKDS
                     FIYLASPMTVAASALKGEISDPREVL"
BASE COUNT          405 a          851 c          802 g          714 t
ORIGIN      
        1 tccacggccg agtagaggtc ctcggtgttc tcccggaaga gcttgatgtc gatcttcggg
       61 tcgcccttca gggggcactc gagccccggc atcagtttcg ccggcctgaa gttgatgtag
      121 aggtcgaaga tctgcctcat ctggaggatg gccgacttga agcccttcac gcccgccttg
      181 gaggtgatgg cggccatgaa ggtggcgtcg gtgtccctga ccttgtcgat ggtctcctgg
      241 ggtacggtgt ttcttctctt gtccccctcg gggaattcct tgcaggactt ctcccacatg
      301 ctccatccca tgtcggccct aacccagtcg atggggggat tgagggcctg catgacgatc
      361 atggcggctt ccattacgtc gtagccggag tcgtcgccgg ggatatagca gacctggtag
      421 cggtccttct tttccttcac ctgcatcaca tttcttgcca tgtgcacttc cccctcctgg
      481 tttcatcaaa ggcggaccga aaacgcgccc tcaccgccgg ctgggatatc tttcccgctt
      541 cttgtgttgc gacccgcctt tgccctaaat ttttacagtc cttgcccttg cttttcgcct
      601 tacgcttttg cctttactct ttcgattccc gatttccgat ttacgtttta cgggttttgc
      661 cctttcgatt cccgattccc gattcacgat ttacgattca cgggttctgc ccttcctgct
      721 tcctgtcgcc tgtctcctgt cgtcttgccc ttccgtctta cgtcttccgt cttacgcttt
      781 tgcttttacg tctttcgtct cacgtcttac gtcttacgtc ttacgtctca cgcttttgcc
      841 ttggctcttt cgattcccga tttccgattc acgttttacg ggttttgctc tttcgattca
      901 cgattcacga tttacgattc acggtttctt tcgcccttac aaccccaaac tcttcttcac
      961 gtggggtatc agccctccgt cctcgatcag gcccttgacg aaatcggggt agggcgggaa
     1021 cttgaaggtc cccttggggg tgacgatctc actcttgtcg aagtcgacgg tgacctcatc
     1081 gcccttctcg agggcgtcga cggcctcggg gctcaccacg atgggcaggc cctcgttcag
     1141 ggcgttgcgg tagtagatcc tggcgaaacc cttggcgatg atggccccga tcccccgcat
     1201 cttgatgcag gtgacggcct gttcccggga ggagccgcag ccccagttct tgccgccgac
     1261 gatgatgtcg cccggctggg cgttcttggt gaactcgggg tcgaggtctt cgagggcgta
     1321 cttgcccatc tcgttacggt ccgagacggt gtaggtgtat ttccccggga atatgacgtc
     1381 ggtgttgatg tcgtcgccgt acttccagac ctttcctgtg accttcatgg cgctaaagca
     1441 cctccctggg atcggatatc tcgcccttta aagcggaggc ggcgaccgtc atggggctgg
     1501 ccaggtagat gaagctgtcc ttgttgccca tgcggccctt gaagttcctg ttggccgtcg
     1561 agatgcaggt ctcacccggc gccagtatcc cctcgtggtt gcccatgcag ggtccgcaac
     1621 ccgggtttac gatgacgcac ccggcgtcga ggaagacctc gatgagccct tcttccagcg
     1681 cctggcggta gaccgtccag gaggccggca gcacgatggt cctcacggcc acttccttgc
     1741 ccttcaggat acctgcggca agccgcaggt cctcgatgcg ggcgttggtg cagctcccga
     1801 ggaaggcctg gtggatgggt gtgcccttga cctcgtcgat gggggcgtag ttgtccaccg
     1861 tgtggggttt ggcgacggcg gggaccaggt cacccaggtc gtagtgcagt tccttggcgt
     1921 agaccgcgtc atcgtcggcc cagatggcct cccactggtc cgtctttgct atgggcttga
     1981 tgaagtccag gaccttctgg tccggggggc agacggcgtt tttggcgccg acctcgatcc
     2041 ccatgttgca gagggtcatc ctctcggata gagacatctc ttcgatggcc gggccgtgga
     2101 actcgatgga catgtagtcg gccccgtcgg cctttatgtc gcccgcgatc ttgagtatca
     2161 ggtctttggc ggagacgccc ttcttgaact tgccagtcac ggtgatcttc atcgattcag
     2221 ggacgcggaa ccagatctgc cccgtcgccc aggtggcggc catctcgctg cggcctatcc
     2281 ccgtggagaa ggtgccgaag gcgccgtagg tgcaggtgtg gctgtcgctg cccacgacga
     2341 tcaagcccgg gagggcgaag ccttcctcgc acatgatctg gtggcagacg ccgcccttgc
     2401 tcgtcacgtc gtagaagtac cggatccctt gttccttgac cacctggcgg atcttcatgt
     2461 ggttctcggc gtgcttatcc gtcggcgcgg ggatggcgtg gtccaggatg aacaccaggt
     2521 ggtcgggttt ccagaccttc ttgacgccta tcttctcgaa ggtgccccag atgggcgcgg
     2581 cgttgtcgtg gctcatgcag aagtgaggct cgacgatgac gacctgtccg gcggaaacgg
     2641 gctcgccggc agccttgccc agtacctttt cagcgaatgt ctttcccatg ccgaccccct
     2701 cctacttgcc cgcggctttt tccttgaggt actcgatgag gccgccggcc ttcatgatgt
     2761 ccatctcgag gt
//
LOCUS       DAOQTR010000305         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_43455_length_2760_cov_3.351571, whole genome shotgun sequence.
ACCESSION   DAOQTR010000305 DAOQTR010000000
VERSION     DAOQTR010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43455_length_2760_cov_3.351571"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKN85_07705"
     CDS             complement(<1..845)
                     /locus_tag="PKN85_07705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT44333.1"
                     /translation="MDEKPYVSVLVPVLNEEESLPELNERLRDALAAIHRPYEIIYIN
                     DGSTDRTEGILETFHREDRRIKVIEFNRNYGQHMALFAGFDFARGEIVVTIDADLQNP
                     PEEIPRLVAKAEEGYEVVATYRKQRKDSLFRKLPSYIVNRITAKLVGVRLKDYGCMLR
                     AYRRNIVDYMNMCPESSSFIPALANTFAKKITEIEVGHEERKKGTSKYSPFKLFRLNF
                     DLMTNFSLLPIQFISMLGVAIAFLGLAFAIFLMVRRLLIGPEGEGTFTLFGVLFFFIG
                     IQIFAL"
     gene            complement(838..2391)
                     /locus_tag="PKN85_07710"
     CDS             complement(838..2391)
                     /locus_tag="PKN85_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT44334.1"
                     /translation="MKIRLTALIILAGILFFFNLGATSLWDPDEPRQAIMAREMMDRG
                     DYIRPYLNGIPYLEKPPFYSWMIVIAAKMSGTVDEFASRAPSALAALLLVVMTFLLGC
                     RLGDERAGFFSGLVLATNYQFLSNARESVMDMTFAFFIGLAIYLAFISLEKGKRLNFA
                     LSFIPGAFAILTKGPAGLVIPVGVMFVYLIFRRGLRRYIIPLVLGCLLAAAIASVWFL
                     LAGESYIKEFILHQNVTRYTNAFDHLESPWCYFHKLFFNFMPWSLFLPFALFGAWKRR
                     YVLPLLWFVLIFLFFELSQSKRAIYLLSAYPALALIVGLFIRDSWDSLIEKGWTNTVL
                     KVFAVLLALIPIGAVVAMYVLPEEDVAVFREGPVSLYVYLAVLFLAACAFLVALFKRS
                     RHFSLASFLFFLVFTGFFYSAYYMPVVDRTSKSLTLITDALGDHRRSKKIYTYGFNSA
                     GIIFYVGKPVKALSDIKEIKEDESDILLIVEEKPTMHLRETLEKSFVPLKRVKYEKEH
                     YIFYVRKNG"
     gene            complement(2564..>2760)
                     /locus_tag="PKN85_07715"
     CDS             complement(2564..>2760)
                     /locus_tag="PKN85_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44335.1"
                     /translation="WSSPLIVVTAIRVALHLPDALPCVPHDTRSPAAGQAALPFRVVS
                     DIFLFILHKAGVLFQFSFFA"
BASE COUNT          685 a          725 c          809 g          541 t
ORIGIN      
        1 agggcgaaga tctgtatgcc gatgaagaag aagagcacgc cgaagagggt gaaggtcccc
       61 tccccttcag ggcctatgag gagccgtcgc accatgagga agatcgcgaa ggcaagaccg
      121 agaaaggcga tggccacacc gagcatgctg atgaactgta tgggcaggag cgaaaagttc
      181 gtcatgaggt cgaaattgag cctgaagagc ttgaaggggc tgtacttgga cgtccccttc
      241 ttcctttcct cgtggccgac ctctatttcc gttatcttct tcgcaaaggt attggcaagc
      301 gccgggatga agctcgacga ttcgggacac atgttcatgt agtcgacgat gttacgccgg
      361 tacgccctga gcatgcagcc gtagtccttg aggcggacgc cgacgagctt cgctgtgatc
      421 ctgttgacta tgtaggaagg cagcttgcgg aacagcgaat ccttcctctg cttccggtac
      481 gtggcaacga cctcgtaacc ctcttccgcc ttggccacga gcctcgggat ctcttcgggc
      541 gggttctgca gatcagcatc gatggtcacc acgatctcgc cccgggcgaa atcgaaaccg
      601 gcgaaaaggg ccatgtgctg accataattc ctgttgaact ctatgacctt gatgcgtctg
      661 tcctcgcgat ggaaggtctc gaggatcccc tccgttcggt ccgtgctccc gtcgtttata
      721 tagatgatct cgtagggtct gtgaatggcc gccagagcgt cgcggagccg ctcgttcagt
      781 tccgggagcg actcctcttc gttcagaacg gggacaagca cggacacata aggcttctca
      841 tccatttttc ctcacataga agatatagtg ctccttctcg tacttcaccc tcttcaaggg
      901 cacgaaggac ttttccagag tctccctgag atgcattgtg ggtttctctt cgacaatcag
      961 cagtatatca ctttcgtctt cttttatctc cttaatatcg ctcagggctt tgaccggttt
     1021 gccgacatag aatatgatgc cggcggaatt gaatccgtac gtgtatatct tcttgctcct
     1081 cctgtggtcg ccgagggcgt ccgtgatgag ggtaagggac ttcgacgtcc tgtcgacgac
     1141 gggcatatag taggcgctgt agaagaaacc cgtgaagacg agaaagaaaa ggaaggatgc
     1201 cagggagaag tgcctgctcc ttttgaaaag cgccacgaga aaagcgcagg cagcgagaaa
     1261 gagtacggca agataaacgt acagggacac cggcccttcc ctgaagacgg caacgtcctc
     1321 ctcgggcagc acatacatgg cgacgaccgc gccgatgggt atgagggcga ggagcaccgc
     1381 gaagaccttg aggacggtgt tcgtccaacc cttttcaatg agactgtccc aggaatccct
     1441 gatgaaaagg cccacgataa gcgccagcgc cgggtaggcg gacaggaggt agatcgccct
     1501 cttgctctgg gaaagctcga agaaaaggaa gataagcacg aaccagagaa ggggaagaac
     1561 gtaccgccgc ttccaggccc cgaagagagc gaagggaagg aagaggctcc agggcatgaa
     1621 gttgaagaaa agcttatgaa agtaacacca gggcgactcg agatgatcga aggcgttcgt
     1681 gtagcgggtc acgttctgat ggaggatgaa ctccttgatg tagctctcgc cggctagaag
     1741 gaaccatacg gacgcgatgg cggccgccag gagacagccg agcacgaggg gaatgatata
     1801 gcgcctgagg cccctcctga agatgagata gacaaacatg accccaacgg ggatgacaag
     1861 ccccgccggc cccttcgtga ggatggcgaa ggcgccgggg atgaaggaaa gcgcgaagtt
     1921 caggcgcttg cccttttcca gtgagatgaa ggccaggtag atggcgaggc cgatgaagaa
     1981 ggcaaaggtc atatccatca ccgattccct ggcattcgag aggaactggt aattggtcgc
     2041 gaggacgagt cccgagaaga aacccgcccg ttcgtcgccg agcctgcatc ccagaagaaa
     2101 agtcatcacc acgaggagga gtgccgcaag cgccgagggc gcccgggaag cgaattcatc
     2161 gaccgtcccc gacatctttg ccgcgatcac gatcatccac gaatagaagg gcggcttctc
     2221 caggtagggg atgccgttga ggtagggccg gatgtagtcc cccctgtcca tcatctccct
     2281 cgccatgatg gcctgccgcg gttcgtccgg gtcccacagg gaggtggccc ccagattgaa
     2341 gaaaaaaagg atgcccgcga gaatgataag cgccgtaagt cgtattttca ttttctttcg
     2401 cttcgtaagg atacaaacat ggcaaaatac aggaagtttt atatgccaac acgggacatt
     2461 tttcaacatt tctttactcc aggcaaaccc gcttttccat agacgcaatc atttcgaagg
     2521 gttcccgccc cttcagcgac ccttcggcat cgacacccgg tcatcaggca aaaaaggaga
     2581 actggaaaag aactcccgct ttatgtagaa taaatagaaa tatatctgaa accacgcgaa
     2641 acgggagagc agcctggccg gccgcaggcg aacgggtatc gtgaggcacg cagggaaggg
     2701 cgtccggcag atgtaaggcg acccggatgg cagtgacgac aatcagggga gaagaccatg
//
LOCUS       DAOQTR010000306         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_43674_length_2751_cov_5.555267, whole genome shotgun sequence.
ACCESSION   DAOQTR010000306 DAOQTR010000000
VERSION     DAOQTR010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43674_length_2751_cov_5.555267"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKN85_07720"
     CDS             <1..380
                     /locus_tag="PKN85_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT44336.1"
                     /translation="DDPLTAQQHNDLGVAYEEKGDFALAEKEYEKALKKNRDWVIPYL
                     NLGHLYYRQEQLDRAEHILREGLRTRGDHPDLLNNLAWVLMEKGRLEHAQYLVDKAIA
                     IEDREEYRDTRREILERMKEKQP"
     gene            683..1735
                     /locus_tag="PKN85_07725"
     CDS             683..1735
                     /locus_tag="PKN85_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNT44337.1"
                     /translation="MDREMTRREFLNATAVTGTVLLAGNLFPGISRAQGVVRIPESDS
                     ITITVLADNYVDATRGDDKIAKRLKRVTSPLDTAPHGEHGLAYRIETVVNGQSHCCLF
                     DFGSDPEGMMKNMKLLKVDPAKVEALGISHDHWDHQQAFLGIMQANKDAFRKGIPLYV
                     GEPFFVGTFQRLPTGNVISLLGLNRQDVEGLGFIKIVEIKGPTPFIPGAYFTGKVEQV
                     TDYEKVPPRFVEKKGDDYVPTTFPGEQAVILNAKGKGLVVLSACAHRGIVNTVKHAQK
                     MTGIEKVYMVMGGFHLTGAKPEVIQKTVADIKAINPQYIVPTHCTGFEAVEAFSKEMP
                     NQFIINTVGTRYVIAS"
     gene            complement(1919..>2751)
                     /locus_tag="PKN85_07730"
     CDS             complement(1919..>2751)
                     /locus_tag="PKN85_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNT44338.1"
                     /translation="LPRLVEPLQHFQENQVVGSHGLLSSRVDCRETCGRTSKSCNYHA
                     ARGPPFSLDRYRQIGYFPVDLFPREAGMIRAERTIAKLNAGKDVKIVAIGDSLTYGWQ
                     VSKGYLDFLKEMLRKRYPEASFQIVNKGIPGDTASGGLDRIDSDVYFYEPDCVLVEFA
                     LNDAFCGYSSELFGRNIQEMIAGIRKNTKAEVVLVTAVWLDDPQSYDFVEKFFYGKLE
                     EMAQKFGLPIARVHERWRRQVLSGAVDFQELVQFDGVHPTVEGYRLMAEAVMEVFSDS
                     "
BASE COUNT          605 a          806 c          802 g          538 t
ORIGIN      
        1 tcgacgaccc gctgacggcc cagcagcaca acgacctcgg ggtggcctac gaggagaagg
       61 gcgacttcgc cctggccgag aaggaatacg agaaggccct gaagaagaac cgggactggg
      121 tgatccccta cctgaacctc gggcacctct actaccggca ggaacagctc gaccgggcgg
      181 agcacatcct ccgcgagggc ctgcggacca ggggcgacca ccccgacctg ctcaacaacc
      241 tcgcctgggt cctcatggag aagggccggc tggaacacgc gcagtacctc gtcgacaagg
      301 ccatcgccat cgaggacagg gaggaatacc gcgacacccg ccgggagatc ctcgagcgca
      361 tgaaagagaa gcagccctga agctctcctg cgcatgtctt cctggggtgc tgcttttcgt
      421 gttctctccg agaatgccct ttattttctt gacggctgtt gtaatcgaga gcatagtgat
      481 cgtgctgttg cgtcttcaac accgtcaccc cccaatcccc cctaaccttc aggagtgcag
      541 catgtccggg cgacacctga aggtgcccac gcaagcgccc cattgggatt cgtgaccctc
      601 tggggctgcc gtctccacgt ctgcatgact ccagatttca aatccatctt gtgtttcgta
      661 tgccgcacga gaggagggtt ctatggacag ggaaatgact cgcagagagt ttctgaatgc
      721 cacggctgtc acgggcacgg ttcttcttgc ggggaatttg tttcccggga tctcccgggc
      781 gcagggtgtg gtcaggattc ccgagtccga cagcatcacc ataacggtcc tggcggataa
      841 ttatgtcgac gccacccgcg gcgacgacaa gatcgccaag cgactcaaac gggtcacgtc
      901 tcctctggac accgcaccgc acggcgagca cggtcttgcc taccggattg aaactgtcgt
      961 caacgggcag tctcactgct gcctcttcga tttcggctcc gatcccgagg gaatgatgaa
     1021 aaacatgaag ctgctgaagg ttgacccggc gaaagtcgaa gcccttggga tcagtcacga
     1081 ccattgggat catcagcagg ctttcctcgg gatcatgcaa gcgaacaagg atgcattccg
     1141 gaaaggcatc cccctttacg tgggcgaacc attcttcgtg gggacctttc agaggttgcc
     1201 caccgggaac gtcatcagcc tgctgggcct gaacaggcag gatgtcgaag ggctcgggtt
     1261 tatcaagatc gtggagataa aggggcccac tcccttcatc cccggcgcct acttcacggg
     1321 caaggtcgag caagtgactg actacgaaaa agtccccccg cggttcgtgg agaaaaaagg
     1381 agacgactac gtgccgacga ccttccccgg cgagcaggcc gtaatcctca atgccaaggg
     1441 gaaggggctg gttgtacttt ccgcatgcgc ccacaggggg atcgtaaaca cggtgaaaca
     1501 tgcacagaag atgaccggca ttgaaaaagt ctacatggtg atgggtggat ttcacttgac
     1561 gggtgcaaaa ccggaggtca ttcaaaaaac ggttgccgac atcaaggcca tcaatccaca
     1621 atacattgtg ccgacccatt gcaccggatt cgaggccgtc gaagcattca gcaaggaaat
     1681 gcccaatcag ttcatcatca acacggtcgg gacacggtat gtcattgctt cctgagcatg
     1741 gccgtcgttg gctcggctcg ctctggaacc aacaaaagca gggtcagaaa tggccctgct
     1801 tcattttcag catttcaaga gcaaatccgc agatcctttt atccatttcg tgcatgaggc
     1861 agtaggtgat gtcggcacgc cgtatctgga attccagaca agtcattcat cttctgcatc
     1921 atgaatcgga aaacacctcc atcaccgcct cggccatgag gcggtagccc tccaccgtcg
     1981 ggtgcacccc gtcgaactgg acgagttcct ggaagtcgac ggcgccggag agcacctgcc
     2041 tgcgccagcg ctcgtggacg cgggcgatgg gcaggccgaa tttctgcgcc atctcctcga
     2101 gcttgccgta gaagaacttc tcgacgaagt cgtaggactg agggtcgtcg agccagacgg
     2161 ccgtgacgag gacgacctcg gccttcgtgt tcttccggat cccggcgatc atctcctgga
     2221 tgttgcggcc gaagagctcc gacgagtagc cgcagaaggc gtcgttgagg gcgaactcga
     2281 caaggacgca gtccggctcg tagaagtaga catccgaatc gatccggtcg agcccccccg
     2341 aggccgtgtc gccggggatg cccttgttga caatttggaa cgacgcctcc gggtagcgct
     2401 tccggagcat ctcctttaga aagtcgaggt atcctttgct gacctgccag ccgtaggtaa
     2461 gggagtcgcc gatggcgacg atcttcacgt ccttgcccgc attgagcttc gcgatcgtcc
     2521 tctccgcccg gatcatgcct gcctcccgtg gaaaaaggtc tacgggaaaa tacccgatct
     2581 gccgatacct gtcgagggaa aagggcggcc cgcgcgctgc atggtaattg catgattttg
     2641 atgttcggcc gcaggtttcg cggcaatcca cccgggagga aagaagacca tgggatccga
     2701 caacctgatt ttcctggaag tgctggagtg gttcgacgag acggggcagg a
//
LOCUS       DAOQTR010000307         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_44238_length_2729_cov_4.009723, whole genome shotgun sequence.
ACCESSION   DAOQTR010000307 DAOQTR010000000
VERSION     DAOQTR010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44238_length_2729_cov_4.009723"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKN85_07735"
     CDS             complement(<1..544)
                     /locus_tag="PKN85_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT44339.1"
                     /translation="MEVYSLKNGLNYIFEERRNTGVVAIQVWVNVGSKDEDDRVAGIT
                     HFIEHLIFKGTEKLKGNAFASRIEAMGGSVNAFTSFDNTVYHIVIPSRVFREGFELLM
                     ESVRHPAFPKEEIAKERKVILEEIKMGEDDPQRSLFKELFSLSYHGEAYGKPVIGFAR
                     TVERISRQDIVDYFRSHYVPA"
     gene            complement(683..1276)
                     /locus_tag="PKN85_07740"
     CDS             complement(683..1276)
                     /locus_tag="PKN85_07740"
                     /inference="COORDINATES: protein motif:HMM:NF005325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNT44340.1"
                     /translation="MKKDEKTTNIFLSGVGGQGAILASNILGEVFIRAGYDVKKAEVH
                     GMAQRGGDVTTHFRFGKKVYSPIIKYGDVDFLLSFELLEAIRYINWVKPDGKIVINTQ
                     TILPPSVSLGQMQYPQGVEDTFKKHFKDNVHVVDGLGIAKELGNLQAQNVVLVGAFSN
                     FFPKMKEAYFIDAIKKLLAPKLHDLNIRAFEAGRKAL"
     gene            complement(1287..>2729)
                     /locus_tag="PKN85_07745"
     CDS             complement(1287..>2729)
                     /locus_tag="PKN85_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT44341.1"
                     /translation="FGKVPMLEPDDAQECLDFTKAAFDISEKFDTPVLLRTVTRIAHA
                     DSLVQTGKRVESKMPLGLDPKEAPKYVMVPAHVRPRRKAVEERMKKLEAFADKTKINK
                     MEINSKSVGIIASGSAYMYAKEVFPEYSFLKLGMVWPLPKKMIADFFKQVKKVMIIEE
                     LDPFFETEIRAMGYKVFHGKDIIPNMYELSPEIIEQAVKGKAYKAPKVRVKPEDLPRR
                     PPNLCAGCSHRPLFYALKKLGAFVFGDIGCYTLAVAPPISALHTTICMGAGTGMEHGA
                     MKAMGKEALGKMVAVLGDSTFLHSGIAPLMNAAYNKGTTTTIVLDNRITGMTGHQEHA
                     GTGFTVRGEVANQVDYAEIGKALGVKSIRYVDPYNIKETMEVVKEEMNKDEASLILCK
                     DSPCMLLRRAKPLDKFKNPLYAINLDKCRGCKMCLEINCPAISWREGAGQTADGQKRK
                     GTAYINPDQCVGCEVCVQVCKFDAIEPAKK"
BASE COUNT          521 a          758 c          778 g          672 t
ORIGIN      
        1 tggcaggcac ataatgggac cggaaataat cgacgatgtc ctgtctcgag atcctctcca
       61 cggtccgggc aaagccgatg acgggtttcc cgtacgcctc tccatggtag ctcagggaga
      121 agagctcctt gaaaagactg cgctgaggat cgtcctcgcc catttttatc tcctcgagga
      181 tgaccttgcg ttcctttgct atctcctcct tggggaaggc ggggtgccgg acggattcca
      241 tgagcagctc gaagccctcc cgaaagaccc gggacggtat gacgatatgg tagacggtgt
      301 tgtcgaagga tgtgaaggca ttgacgctcc cccccatggc ctctatccgg gaagcgaagg
      361 cattgccctt gagcttttcc gtccccttga agatgaggtg ctcgatgaag tgcgttatac
      421 ctgctacccg gtcgtcctca tccttgctgc caacattcac ccacacctga atggcgacga
      481 caccggtgtt cctgcgttct tcgaagatat agttgaggcc gttcttgaga gaatagactt
      541 ccatggctgc cgtccttccg ggaaatgaga acatcacgag caagacaagg gcaatgaacg
      601 acaggaatga gaaaggcccc cgaaagggcc tttcttcctg tttaaaacgt atgaaacgta
      661 aagtcatata cttcttcggt tgctacaggg cctttcttcc cgcttcgaag gccctgatgt
      721 tgagatcgtg cagcttgggc gccagaagct tcttgatcgc gtcgatgaaa taggcttcct
      781 tcatcttcgg gaagaaattg gagaacgcgc cgacgaggac gacgttctgg gcctgcaggt
      841 tgccgagctc tttcgctatg ccgaggccgt cgacgacatg gacattgtcc ttgaaatgct
      901 tcttgaaggt gtcttccacg ccctggggat actgcatctg gccgaggctt accgacgggg
      961 gaaggatcgt ctgggtgttg atgacgatct tgccgtccgg ctttacccag ttgatgtagc
     1021 gaatcgcttc gaggagctcg aaggaaagga ggaagtcgac atcgccgtac ttgatgatcg
     1081 gcgaatagac cttcttgccg aaacggaaat gggtggtgac atccccgccc ctctgggcca
     1141 tgccgtggac ttccgccttc ttgacatcgt atccggcccg gatgaagact tcaccaagta
     1201 tgttgctcgc aagtatggca ccctgcccgc ctacaccgga aagaaatatg tttgtggtct
     1261 tttcatcttt tttcatatcc gcctcctcac ttcttagccg gttctatggc gtcgaattta
     1321 catacctgta cgcagacttc gcagccaaca cactgatcgg gattgatata ggccgtgccc
     1381 ttcctcttct ggccgtctgc cgtctgaccc gcgccttccc gccagcttat ggcaggacag
     1441 ttgatctcga ggcacatctt gcagccccgg cacttgtcca ggttgatggc atacagagga
     1501 ttcttgaact tatccagagg tttcgccctg cgaaggagca tgcaggggct gtccttgcag
     1561 agaatgaggg acgcttcgtc cttgttcatc tcttccttga cgacctccat cgtttccttg
     1621 atgttgtagg gatcgacgta gcggatgctc ttgacgccca gggccttgcc gatctcggcg
     1681 tagtccacct ggttcgccac ttcgccgcga acggtaaagc ccgttcccgc gtgttcctgg
     1741 tgacctgtca tacccgtgat cctgttgtcg aggacgatcg tggtggtggt acccttgttg
     1801 taggcggcgt tcatgagggg agcgatcccg gagtggagga acgtggagtc gccgaggacg
     1861 gctaccatct ttccgagagc ttccttgccc atcgccttca tggcgccatg ctccataccg
     1921 gtgcccgcgc ccatacagat ggtcgtgtga agggcggaga tcggcggggc gactgccagc
     1981 gtgtagcagc cgatgtcgcc gaagacaaag gcgcccagtt tcttcagggc gtaaaagaga
     2041 ggcctgtgcg agcagccggc acagaggttc ggcggccttc tcggcaggtc ttccggtttc
     2101 accctgacct tcggggcctt gtaggctttg cccttcacgg cctgctcgat gatctccggt
     2161 gagagttcgt acatgttggg gatgatgtcc ttgccgtgga agaccttgta gcccatcgcc
     2221 ctgatctctg tttcaaagaa ggggtcgagt tcttcgatga tcatgacctt cttcacctgc
     2281 ttgaagaaat cggcgatcat cttcttcgga agcggccaca ccatgccgag cttcaggaag
     2341 gagtattccg ggaagacttc cttggcatac atgtaggcgg aaccactggc gatgatgccg
     2401 acgctcttgg agttgatctc catcttgttg atcttcgtct tgtcggcgaa ggcttccagc
     2461 ttcttcatgc gctcctcgac ggcctttctt ctcggccgga cgtgggcggg gaccatgacg
     2521 tacttcggcg cctctttcgg gtcgagtccg agaggcatct tggattcgac tctcttgccc
     2581 gtctgaacga gtgaatcggc gtgagcgatc cttgtcaccg ttctcaggag aacgggggtg
     2641 tcgaacttct cgctgatgtc gaatgcggcc ttcgtgaaat caaggcattc ctgggcgtcg
     2701 tccggttcca gcatgggtac cttgccgaa
//
LOCUS       DAOQTR010000308         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_44259_length_2728_cov_3.642724, whole genome shotgun sequence.
ACCESSION   DAOQTR010000308 DAOQTR010000000
VERSION     DAOQTR010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44259_length_2728_cov_3.642724"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..>928)
                     /locus_tag="PKN85_07750"
     CDS             complement(22..>928)
                     /locus_tag="PKN85_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT44342.1"
                     /translation="VSSLVVRIAKTDSTCIISGESGVGKEVVLNMIHALSERKDFPLV
                     KVNCAAIPETLLESELFGYEKGAFTGAYQRKAGKFELANKGTIFLDEIGDIPLVLQSK
                     LLRVIQEKEVERLGGAHPVKIDVRIVAATNRNLEEEVKKGTFREDLYYRLNVVNIVIP
                     PLRERREDIPLLIDFFLKKYNLKHKKNVKGLTREARDTMVKYDYPGNVRELENIVERA
                     IVLTRGDHITSQDLPNLAEQAMVTGDGSIRGTVESIERSMIIEALINADWVQTKAAMA
                     LGLSERMLRYKIKKYGISRSSRSAG"
     assembly_gap    929..938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<939..1262)
                     /locus_tag="PKN85_07755"
     CDS             complement(<939..1262)
                     /locus_tag="PKN85_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44343.1"
                     /translation="MDRVGILIVEDEQTQRSLLGGLLRKEGYTIGEASDGESALALFQ
                     KDIFEIVLLDYRLPDTDGLSLLKRIKEINPATEVVMVTAFGSIENAVGALKAGASEYL
                     TKPIDL"
     gene            complement(1285..>2728)
                     /locus_tag="PKN85_07760"
     CDS             complement(1285..>2728)
                     /locus_tag="PKN85_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44344.1"
                     /translation="FTHVQREIDINLEYLSFLDRSPVIITPYFFNIMAFDESIIDDLD
                     AVMGRFLKNPAERGLEELSLANVIEYDLTGKIVAEKGRSDIPAGLLQKIVSGEEATVI
                     RMPVAGDRSLVFGRRLGERILFVRINAPELETLRRKVILRDIVDREEKRFNIDSIRIY
                     DEKGGLFVGTSDAGKNTFLLERDLSSKVLPGFRMQIFISRNLARDTIQRSAIGFILIL
                     AVLFVSGAGSIYAFFVMERKYAKRVKDMEREMEIKERLVSLGKLASGMAHEIRNPLNA
                     ISLSVQRLKREFVPWDEKKEEYLTFLDIIRKELTRVNGIVEEFLQSTRAQAPFSREDL
                     RDLLEEVIIIVGEKASSKGIFIENATGTGIHVDCQRDRLKQAFYNIIINAIESMKDGG
                     KIAITVKQRDSRVEISVRDSGPGISEEALARIFEYYYTTKDKGMGLGLPISYVIVKDH
                     GGDIKVTSAAGQGTTFVIVLPRTQDATTAA"
BASE COUNT          494 a          783 c          756 g          685 t
ORIGIN      
        1 cccgtgggcc cgtttgcgcg atcagcccgc ggagcgggat gatcgcgata tgccgtactt
       61 cttgatctta tacctcagca tgcgctccga cagaccgagg gccatcgcgg ccttcgtctg
      121 gacccagtcg gcattaatga gggcttcaat gatcatcgat ctctcgatgg attcgacggt
      181 tccccgaata ctgccgtcgc ctgtcaccat tgcctgctcc gccaggttcg gaaggtcctg
      241 gctggtgatg tggtctcccc gggtaaggac tatggccctc tccacgatgt tctccagttc
      301 cctgacgttg cccgggtagt cgtatttgac catggtgtcg cgtgcctccc gcgtgagacc
      361 cttgacgttc ttcttatgct tgaggttgta tttcttcagg aaaaaatcga tgagcagcgg
      421 aatgtcctcc ctgcgctccc ttaagggagg gatgacgata ttgacgacat tgaggcgata
      481 gtacaggtct tcccggaatg ttcccttttt gacctcctcc tccaggttgc gattggtcgc
      541 ggcgacgatg cggacatcga tcttgacggg atgcgcccct ccgaggcgct cgacctcttt
      601 ctcctggatc accctgagga gctttgattg aagcacgagg gggatgtcgc ctatctcgtc
      661 aagaaagatg gtccctttgt tggcgagttc gaacttgccg gccttgcgct ggtaagcgcc
      721 ggtgaaggcg cccttttcat agccgaagag ttcactctcc aggagcgtct cgggaatggc
      781 ggcgcaattg accttgacga gggggaaatc cttgcgctcg ctgagcgcgt ggatcatgtt
      841 gaggacgacc tcctttccaa caccgctctc gccgcttata atgcacgttg aatccgtctt
      901 cgctatcctg accaccaggc tcgagaccnn nnnnnnnnga ggtcgatggg tttcgtaagg
      961 tactcggacg ccccggcctt gagggccccg acggcgttct ctatgctccc gaaggcagtg
     1021 accatgacca cctccgtcgc cgggtttatc tcctttatcc ttttgaggag ggacaggccg
     1081 tccgtgtccg gcagtctgta gtcgagaagc acgatctcga aaatgtcttt ttgaaaaaga
     1141 gcgagggcgc tttctccgtc ggatgcctca cctatcgtgt agccttcttt cctgagcagc
     1201 ccgccgagga gcgatctctg tgtctgttcg tcttccacga tcaatatgcc gaccctgtcc
     1261 ataaccgcaa cccctccttt ccctttacgc cgccgtcgtg gcgtcttgcg tgcggggcag
     1321 gacgatgacg aaggtggttc cctgcccggc agcgcttgtc accttgatgt cgccgccatg
     1381 gtctttcact atcacgtacg atatgggcag tccgaggccc atacccttgt cctttgtggt
     1441 gtagtagtat tcgaatatcc tcgccagcgc ctcttcactg atgccgggcc cggaatccct
     1501 gaccgatatt tcgacgcggg agtctctctg cttcaccgtg atggcgatct ttccgccgtc
     1561 cttcatggac tctatggcat ttatgatgat gttgtagaat gcctgcttaa gcctgtccct
     1621 ctggcaatcg acgtggatgc cggtgcctgt ggcgttctcg atgaagatac ccttcgatga
     1681 ggccttttcg ccgacgatta tgatcacctc ttcaagaagg tcgcgcaggt cttccctgct
     1741 gaagggggcc tgcgccctgg tcgattggag gaactcctcg actatcccat tgacccgcgt
     1801 gagctccttc ctgatgatat cgaggaaggt gaggtattct tccttcttct cgtcccaagg
     1861 gacgaattct ctcttgaggc gctgaacgga aaggctgatg gcgttcaggg gattcctgat
     1921 ctcgtgggcc atcccggaag ccagttttcc cagcgatacc aggcgttcct tgatctccat
     1981 ctcccgctcc atatccttga cccttttcgc gtacttcctc tccatgacga agaaggcgta
     2041 gatgctcccg gctccggaga cgaagaggac cgcaaggatg agtatgaagc cgatggccga
     2101 cctctggatg gtgtcccgtg caaggtttct cgagataaag atctgcatgc gaaagccggg
     2161 gaggaccttc gaggaaagat cccgctcgag aaggaaagtg ttcttcccgg catcgctggt
     2221 cccgacgaag agaccgcctt tctcgtcata gatcctgatg gagtcgatgt tgaacctctt
     2281 ctcctctcta tcgacgatat ccctgaggat gacctttctt cttaatgttt ccagttccgg
     2341 ggcgttgatc cttacaaaaa ggatgcgttc acccagacgc ctgccaaaga cgagcgacct
     2401 gtcgccggct acgggcatcc tgatgacggt cgcctcctcc cccgagacga ttttttgcag
     2461 gagaccggcg ggaatgtcgc ttctcccttt ttcggcgacg atcttacccg tgaggtcgta
     2521 ctcgatgacg ttcgccaggg agagttcttc caggcccctt tctgccgggt tcttgagaaa
     2581 ccggcccatg acggcgtcaa ggtcgtcgat gatcgactcg tcgaaggcca tgatgttgaa
     2641 gaaatagggg gtgatgatga cgggtgacct gtccaggaag ctgaggtact cgaggttgat
     2701 gtctatctct ctctggacat gggtgaag
//
LOCUS       DAOQTR010000309         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_44260_length_2728_cov_3.506921, whole genome shotgun sequence.
ACCESSION   DAOQTR010000309 DAOQTR010000000
VERSION     DAOQTR010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44260_length_2728_cov_3.506921"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKN85_07765"
     CDS             complement(<1..429)
                     /locus_tag="PKN85_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNT44345.1"
                     /translation="MGGSYEYYRPATIDEAMALMEGSGGNGVYIAGGTDVMVLIRQKK
                     LAPACLISLRNIGELAYLETGGGLTVGSAVTHDEIAKNAFIQRSYSALADATKKVGSL
                     QIRNVATMGGNICNAAPSADTACPLLVLDASARIAGKGGER"
     gene            complement(474..1781)
                     /locus_tag="PKN85_07770"
     CDS             complement(474..1781)
                     /locus_tag="PKN85_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNT44346.1"
                     /translation="MERPFDSKVRSEKELKVLQLDGLKWTVEHAYNGSAHYRRKLDEA
                     GVRPGDIKSLADIHKLPFTTSKDLQEGYPFPLRSVPFEDVVRIHASSGTTGKRKVLCY
                     TAKDIDDWANMFARCYSYADCTPEDRIQIAVGYGVWTAGWGFQNGCERFGAMSIPIGP
                     GNTDMQCQFLEDFGTTAMTCTASMGLLMAELIEEKGLRGKIPLRKMIFGSERASDAMR
                     QRISELSGVAYDQLFDIPGMTELYGPGTGLDCRYHTGIHYWADYYILEILNPETLEPV
                     PEGEVGEMVVTTLRKEAAPLIRYRTRDLTRIMPDKCPCGSLMPRHDRILGRSDDMFIF
                     RAVNIYPSHIDQILSNIKEVGSEYQIRLYRKDKGGKDHMTIRVERSQGTEKSHDADKH
                     IRKAIEKEVKKQVLVSCDVEVEDYGSLPRSERKTKRVFDDRDS"
     gene            complement(1835..>2728)
                     /locus_tag="PKN85_07775"
     CDS             complement(1835..>2728)
                     /locus_tag="PKN85_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNT44347.1"
                     /translation="QVNWGGNVVYNFTYAYVFMAIKSMFAPDIPNNDGCARPIRLTAP
                     EGTVINCRFPAAVAARMGVGHFLTEVIYRALSDVLPGKVIAASGGTPAAMNVFYGKRK
                     DARPWHSVIIRGGGMGAGARNDGNYVYIFPANGANTPVEIFESDTPLIVEKREIVADS
                     GGAGRMKGGLGKREVFRVPDDEYAPIPPVNLGIQAGRYIYPAEGLFGGKPGAKAQFLV
                     NGVPGNSYGLTQLKPGDVVTIDAAGGGGYGSAGERDPEMVLNDVREGYVSPESARSDY
                     KVAVDPKTLEIDEEETRKLRQ"
BASE COUNT          453 a          914 c          743 g          618 t
ORIGIN      
        1 gcgttcccct cccttccctg ctatccgcgc gctcgcgtca aggacaagga gcgggcatgc
       61 cgtatcggcc gacggcgccg cgttgcatat gttgcccccc atggtcgcaa cgttgcgtat
      121 ctgcagggag ccgacctttt tggtcgcgtc ggccagggca ctgtagcttc tctggataaa
      181 agcgttcttc gcgatctcat cgtgggtgac ggcactccct acagtgagac cgccaccggt
      241 ctccaggtag gccagttcgc ctatgttccg cagggaaata agacacgccg gggcgagctt
      301 cttttgcctt atgagcacca taacgtcagt gcccccggct atgtacacac cgtttccgcc
      361 ggacccctcc atcaaggcca tggcctcatc gatggtcgca ggcctgtaat actcatagct
      421 accacccata acccctccta attgttttgc acccgggaat gtccgtccct tgtctacgag
      481 tccctatcgt cgaagaccct cttcgtcttg cgctccgacc tgggaaggga accgtaatcc
      541 tccacctcca cgtcgcagct cacgagtacc tgtttcttga cctccttttc tatggccttg
      601 cggatgtgct tgtcggcgtc gtgggacttc tcggtcccct gtgaccgctc cacccgtatc
      661 gtcatgtggt ccttgccgcc cttgtccttc ctgtagaggc ggatctggta ttcgctgccg
      721 acctccttga tgttggagag tatctggtcg atgtggctgg gatagatgtt taccgccctg
      781 aagatgaaca tatcgtcgct ccgccccagg atcctgtcat ggcggggcat gagactcccg
      841 caggggcatt tgtccggcat gatcctcgtc aggtccctcg tgcggtagcg gatgaggggc
      901 gccgcctcct tgcgtagcgt cgtcaccacc atctccccca cctcgccttc gggcaccggt
      961 tcaagcgttt cgggattcag gatctccagg atgtagtaat cggcccagta gtggatgccc
     1021 gtgtgatagc ggcaatccag gcccgtcccc ggaccgtaga gctctgtcat tcccgggata
     1081 tcgaagagct ggtcgtacgc gaccccggac aactccgata tcctctggcg catggcgtcg
     1141 ctcgccctct cggagccgaa gatcatcttc ctcagaggga tcttcccccg aaggcccttc
     1201 tcctcgatta gctccgccat gagaaggccc atggaggctg tacaggtcat ggccgtcgtc
     1261 ccgaagtcct ccaggaactg acactgcatg tccgtgttgc cggggcctat gggaatggac
     1321 atggcgccga accgctcaca tccgttctgg aaaccccatc ccgccgtcca gacgccgtat
     1381 cccacggcta tctggatcct gtcctcgggg gtgcaatccg cgtacgagta acaccgggcg
     1441 aacatgttgg cccagtcgtc gatgtccttc gccgtgtagc agagaacctt gcgcttgccc
     1501 gtcgttccgc tcgacgcatg gatgcggaca acatcttcaa aagggacgct cctcaaggga
     1561 aaagggtacc cctcctggag gtccttgctc gtcgtgaagg gcaacttgtg aatgtccgcg
     1621 agggacttta tgtcccccgg cctcacccct gcctcgtcga gctttcgccg gtagtgcgcg
     1681 gaaccgttgt acgcgtgttc gacggtccat ttcagcccat cgagctggag aactttcagt
     1741 tccttttccg atctcacttt tgaatcgaat ggccgctcca tgttcactgt cctcctcttg
     1801 actctgacat gcctttcgcg cggcatgccg ttcttcattg tctcagtttc ctcgtctctt
     1861 cctcgtcgat ctcgagcgtc ttcggatcga cagccacttt gtagtccgac ctcgcgcttt
     1921 cagggctcac atatccctcg cggacgtcat tgagcaccat ctccgggtcc cgttcaccgg
     1981 cgctaccgta gccgccgccg ccagcggcgt ctatggtcac cacgtcgccg ggcttaagct
     2041 gggtcagccc gtaggagttc cccggcacgc cgtttaccag gaactgcgcc ttcgccccgg
     2101 gctttcctcc gaaaaggccc tctgccggat agatgtaacg gcccgcctgg atccccagat
     2161 taacaggggg aatgggggca tactcatcat cggggaccct gaacacctct cgcttcccca
     2221 gcccgccctt catcctgccg gcaccgccgg aatcggcgac tatctcccgt ttctccacaa
     2281 taagcggcgt gtcgctctcg aagatctcga cgggcgtgtt tgccccgttg gccgggaaga
     2341 tatacacgta gttcccgtcg ttgcgcgcgc ccgcgcccat gccgccgccc ctgatgatga
     2401 cactgtgcca gggcctcgcg tcctttcgct ttccatagaa gacgttcatc gccgccggcg
     2461 tgccccccga agcggctatc acctttccgg gcagcacatc ggagagcgcc cggtagatga
     2521 cctccgtcag gaagtggccc acgcccattc gcgccgccac cgccgcggga aacctgcaat
     2581 tgatgacggt gccctcgggc gccgtgaggc ggatgggcct tgcgcacccg tcgttgttgg
     2641 ggatgtccgg cgcgaacatg ctctttatcg ccatgaaaac gtaagcatag gtgaaattgt
     2701 agaccacgtt gccgccccag ttgacctg
//
LOCUS       DAOQTR010000310         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_44447_length_2721_cov_3.013503, whole genome shotgun sequence.
ACCESSION   DAOQTR010000310 DAOQTR010000000
VERSION     DAOQTR010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44447_length_2721_cov_3.013503"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKN85_07780"
     CDS             complement(<1..573)
                     /locus_tag="PKN85_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNT44348.1"
                     /translation="MDWLPAILAGLPIAIVGSLMVFLSWPAKWAMPVGWTSAALIAGL
                     WWNMPVRWVAASTIAGFINALDILLIVLGALLILQTLRKSGALEAISFSMSSVSKDRR
                     IQAIIIAWLMVSFLEGAAGFGTPAAIAAPLLVGLGFPPLVAVICTLIGDSTAVTFGAV
                     GVPIWGGFEPVRDLVGLPPGGDFTDFLRIVG"
     gene            complement(595..960)
                     /locus_tag="PKN85_07785"
     CDS             complement(595..960)
                     /locus_tag="PKN85_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNT44349.1"
                     /translation="MIRVKRVYDAPAGDDGYRVLVDRLWPRGMSWERSRIDLWLKDIA
                     PSNQLRRWFHHEPEKWAEFKQRYFAEIRDRTDVLGSLFEKLKTERVTLLYGARDRQFN
                     NATALKEYLENKPAADDKV"
     gene            complement(935..1516)
                     /locus_tag="PKN85_07790"
     CDS             complement(935..1516)
                     /locus_tag="PKN85_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNT44350.1"
                     /translation="MLPIGPLMIEHRLIEKMIGLIQRRADRYAREGDIDPVFIDSVID
                     FMRTYADRCHHGKEEEILFRELNKKVLPPELKHILDQLVEEHRQGRQTVADLVEARQE
                     YARGDREAVRTILDRLRYMTDLYPRHIEKEDGHFFIPCMSLFTQEENEEMLNEENAFD
                     RELVHQIYREKISAWSKESDGEEDTRDTREKGL"
     gene            complement(1631..2080)
                     /locus_tag="PKN85_07795"
     CDS             complement(1631..2080)
                     /locus_tag="PKN85_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT44351.1"
                     /translation="MFAPKNILVPTDFSEFSDNALEKAYDIAKQYKAKIHLLHVIGVV
                     QTCAVDYCFDQATIDDLDRKAAESSKEIMRKQIEKVVKSKDVEIAAYVTKGTPYEEIL
                     KEQQSRNIDLIVIASHGRTGILGHLIGSVAEKVVRAATCPVVLVKNR"
     gene            complement(2103..>2721)
                     /locus_tag="PKN85_07800"
     CDS             complement(2103..>2721)
                     /locus_tag="PKN85_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44352.1"
                     /translation="AKHEFPFKSIPPFAEERGTRQREMDLQQIIRNIQMLLPQELQRN
                     IELILAEQELPVMIDMAQLQAALLNLIRNAQEAMPEEGRITISTGSARLYGDSAGKRG
                     RGSPKTWAFVSISDTGSGIDEETRSRIFEPFYTTKGDPHQGLGLPMASRTIRQHNGTI
                     DIETRSGHGTTVKVYLPFAGRKPARRKAIPLPPAGTAIVAPLRQR"
BASE COUNT          504 a          822 c          748 g          647 t
ORIGIN      
        1 ccccacgatc cgaaggaaat cggtgaaatc ccctccggga gggaggccga ccagatccct
       61 gacgggctca aacccacccc agatcggtac ccctactgcc ccgaaggtaa cggcggtact
      121 gtcgcctatg agcgtgcata tgacggccac gaggggcgga aatcccaacc cgacgagaag
      181 cggcgccgca atggccgcgg gagtgccgaa accggcggct ccttccagga aggagaccat
      241 gagccacgct attatgatcg cctgtattct ccggtctttt gaaacagagg acatggaaaa
      301 ggaaatggct tcgagggcgc cgctctttct aagcgtttga agtatcagaa gggcgcccag
      361 tacgatgagg agaatatcga gggcattgat aaacccggcg atcgttgagg cggcgaccca
      421 tcggaccggc atgttccacc aaagaccggc gatcaacgcg gccgaggtcc aaccgaccgg
      481 catcgcccat ttggcgggcc aggaaaggaa aaccataagg gaacccacta tggctatggg
      541 aagacccgca agaatagccg gtaaccagtc catgcgccac ccgacctccc tcgctcatac
      601 tttgtcatct gctgccggct tgttctccag gtactctttc agcgctgtcg cgttgttgaa
      661 ctgccggtct ctcgcgccat agagaagggt cacacgctcc gtcttgagct tttcgaacag
      721 cgaccccagc acatcggtcc tgtccctgat ctcggcaaag tacctctgct tgaactcggc
      781 ccacttctcc ggttcgtgat gaaaccatct ccgcagctgg ttgcttggcg cgatgtcctt
      841 cagccacaga tcgatgcggg acctttccca gctcatgccc cgcggccaga gtctgtccac
      901 caggaccctg tatccgtcat cccccgcggg ggcgtcatag acccttttca cgcgtatcac
      961 gggtatcttc ctcaccgtcg gattccttgc tccaggcact gatcttttcc ctgtatatct
     1021 gatggaccag ctccctgtcg aacgcgtttt cctcgttgag catctcttcg ttctcttcct
     1081 gggtgaaaag cgacatgcag ggaatgaaga aatgcccgtc ctccttttct atgtgcctcg
     1141 ggtaaaggtc ggtcatataa cgcaggcgat cgaggatcgt tcgtacagcc tctctatcgc
     1201 cacgcgcgta ttcctgtctc gcttcgacca ggtccgcaac cgtttggcgg ccctgccggt
     1261 gttcctcgac cagctggtcc aggatgtgtt tcagctccgg gggaagcact ttcttgttca
     1321 gttccctgaa gaggatctct tcttcttttc cgtggtgaca cctgtcggca taggtgcgca
     1381 tgaagtcgat gacggagtcg atgaatacgg ggtcgatgtc accttcccgc gcgtacctgt
     1441 cagctcttct ttggatcaaa cctatcatct tctctatcag tctgtgttcg atcatcaagg
     1501 gaccgatcgg cagcatcgga gacctcctcc tgtttgatgt cgtccgctcc gcatcgcctt
     1561 ccggtaacct ccgcgatatc ggggcgaagc gcagagcctc gttccggggc cgcgcgtcct
     1621 ttgtcgcccc ttacctgttc ttgaccagga ccaccgggca cgtggctgcc cgcaccacct
     1681 tttccgccac gcttccaatg agatggccca gtatccccgt cctgccatga gacgctatca
     1741 cgatgaggtc gatgttcctg gactgttgtt ccttcaatat ctcctcgtaa ggtgttcctt
     1801 tcgtcacata ggccgcgatc tccacgtcct tcgacttgac gaccttctcg atctgcttcc
     1861 gcatgatctc cttcgaggat tcggccgcct tcctgtccag gtcatcgatg gtcgcctgat
     1921 cgaaacaata atcgacggcg caggtctgga cgacgccgat gacatggagg agatggatct
     1981 tcgccttgta ctgtttcgcg atgtcgtagg ccttttccag ggcattatcg gagaactcgg
     2041 agaaatccgt gggtaccagg atattctttg gtgcgaacat agatgcctcc ttaaaggttt
     2101 attcaccgct gccgcagcgg cgcgacgatg gctgttcccg ccgggggaag aggtatcgcc
     2161 ttgcggcgcg cgggcttcct tccggcgaac gggagataga ccttcaccgt ggttccgtgt
     2221 ccggatctgg tctcgatatc gatggtcccg ttgtgctgcc ggatggtgcg tgaggccatg
     2281 gggaggccca gtccctggtg cgggtctccc tttgtcgtat agaaaggttc gaatatcctg
     2341 gatcgtgttt cctcgtcgat cccggagccg gtatccgaga tggagacgaa ggcccaggtt
     2401 ttggggctgc cgcggcctct cttcccggcg gaatcaccgt agagcctggc ggaacccgtg
     2461 ctgatcgtaa tgcgtccttc ttccggcatg gcctcctggg cgttcctgat gaggttcaga
     2521 agggcggcct ggagctgtgc catgtcgatc atcacaggca attcctgctc cgcaagtatc
     2581 aactcgatat ttctttggag ctcctgcggc aggagcatct gtatgttgcg tataatctgt
     2641 tgcaggtcca tttctctctg cctcgtaccc ctctcttcgg caaagggcgg gatgctcttg
     2701 aagggaaact catgtttggc a
//
LOCUS       DAOQTR010000311         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_44807_length_2706_cov_2.912486, whole genome shotgun sequence.
ACCESSION   DAOQTR010000311 DAOQTR010000000
VERSION     DAOQTR010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44807_length_2706_cov_2.912486"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKN85_07805"
     CDS             complement(<1..212)
                     /locus_tag="PKN85_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNT44353.1"
                     /translation="MKAKYQDLKFELKDGLGILTLNVPEKLNACGKRTRSELHSFWSA
                     MQSEDRCRVIIMTGAGTSFCAGQDVD"
     gene            complement(234..1838)
                     /locus_tag="PKN85_07810"
     CDS             complement(234..1838)
                     /locus_tag="PKN85_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNT44354.1"
                     /translation="MKEETGKTIDYGKYEGQTFGQILDGLARDVPDKEMIVFNGERMT
                     YGQFHQRVMQIAMAMKKLGIKKGDRVAALFPNCPDFFVVQQAVLYIGAVFVLLSTRYR
                     EYELSYMLKHSQARVLFTIDEYLKTSFTDIIDKVRPELPGLEFCFVMGTKVPSWGRPF
                     EEALNLGKDIDEKLLREDLPKYNDTASILYTSGSTGLPKGVVMTHRAFVFGALQVARR
                     LRITPDDVSLMVVPCSHTLCAFIQFPNALMGRCRIVMMETFEAGQALEMYNKEKISLI
                     YGVPTMFVLMLEHPDFAKTDFSSSRAGYTGGAIIPEELMSGVRYKMNCKLVSVYGLSE
                     CGACSMNDVEDDESLKIGTVGRPLDGVDIVITNEKQEKAPDGEVGEVCLKGPILFTEY
                     YQQPELTKAAYDRNGYFCTGDLGKYLDNGMLAIVGRKKEMIIRGGFNVYPAELEEQIS
                     LIDGVQSVAVVGLPDKVMGEKIVACIIPSPGSNITDKDIIAYCKARFANYKVPNVVII
                     MNEFPVTALGKVQKFKLVEALEAKGI"
     gene            complement(2263..>2706)
                     /locus_tag="PKN85_07815"
     CDS             complement(2263..>2706)
                     /locus_tag="PKN85_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT44355.1"
                     /translation="RLSVKENLLIGTSNAPDMTKARKEEMLEKAYMYFPILKEKESQG
                     GGYLSGGQQQMLAIARGLMCDPKLVLLDEPFEGLAPVIIWELIDIIKKLCETEKMTLL
                     LVEQKAALALKMSDRGYVLEKGLVKCEGTCQFLSESDEVRERCGL"
BASE COUNT          554 a          816 c          709 g          627 t
ORIGIN      
        1 tcatcgacat cctgtccggc gcaaaaggag gtccccgcgc cggtcatgat tatcaccctg
       61 cacctgtcct cggactgcat ggcactccag aatgaatgga gctccgatct cgtacgtttc
      121 ccgcaggcgt tgagcttctc cggaacgttg agcgtcagaa tgcccagccc gtccttgagt
      181 tcaaacttca gatcctgata ttttgctttc ataagaaacc ctgtcgtcgt ttcctatatc
      241 cctttggcct cgagcgcctc tacgagtttg aacttctgga ccttgccgag ggccgtcaca
      301 gggaactcgt tcattatgat gaccacgttc gggaccttgt agttcgcgaa gcgtgccttg
      361 cagtacgcga tgatatcctt gtccgttata ttgctcccgg gactggggat tatgcaggcg
      421 actatcttct cgcccatcac cttgtccggc agaccgacca cggcgacgga ctgcacgccg
      481 tcgatgaggc ttatctgctc ctcgagttcg gcggggtaca cgttgaagcc tcccctgatg
      541 atcatctctt tcttgcggcc cacgatggcg agcatgccgt tgtccaggta tttacccagg
      601 tctcccgtgc aaaagtaacc gttccggtcg tacgcggcct tcgtcaactc gggctgctga
      661 tagtactcgg tgaaaaggat cggacctttg aggcagactt cacccacctc gccgtcaggc
      721 gctttctcct gcttctcatt ggtgatgaca atgtccactc cgtcaagcgg acggccgacg
      781 gtcccgatct tcagggattc atcgtcttca acatcgttca tcgagcaggc cccgcattcg
      841 ctcagaccgt acacggacac aagcttgcag ttcatcttgt accgcacgcc ggacatgagc
      901 tcttcgggaa ttatagcccc gccggtgtaa cccgcacgac tgctcgagaa gtccgtcttc
      961 gcgaaatcgg ggtgctccag cataaggacg aacatcgtgg gaacaccata gatgagggat
     1021 atcttctcct tgttgtacat ttcgagtgcc tggcccgctt cgaaggtctc catcatgacg
     1081 atgcggcacc ggcccatgag ggcgttcggg aactggatga acgcgcagag cgtatgcgag
     1141 cagggcacga ccataaggct cacgtcatcc ggagtgatcc tgagccgccg cgccacctgc
     1201 agggcgccaa agacaaaagc ccggtgcgtc atgacgacgc ccttgggcag accggtgctg
     1261 cccgaggtgt agagtatgga agcggtatcg ttgtacttcg gaaggtcttc cctgagcagc
     1321 ttctcgtcga tgtccttgcc gaggttgagg gcttcctcaa acggccgtcc ccacgacggg
     1381 accttggtgc ccatgacaaa gcagaactcc aggccgggca gctcgggacg aactttgtcg
     1441 atgatgtcgg tgaagctcgt cttcaggtac tcgtctatgg tgaacaggac acgtgcctgg
     1501 gaatgcttga gcatgtagct caactcgtac tcccggtaac gggtcgacaa aaggacaaaa
     1561 acggcgccta tatagaggac ggcctgctgg accacgaaaa agtcggggca attgggaaag
     1621 agagcggcaa cacggtcacc tttcttgatg ccgagtttct tcatggccat cgcgatctgc
     1681 atcacccgct ggtggaactg tccgtatgtc atcctttcgc cgttaaagac gatcatttcc
     1741 ttatcgggta catcgcgcgc gaggccgtca agtatctggc caaaggtctg cccttcgtat
     1801 ttgccgtagt cgatagtctt cccagtctct tctttcatgg ctctaccact ccttttcttg
     1861 tgtgatctca ggtttcatca gtacatccaa ctcgtccgac agacatggta ttctctactc
     1921 tgctcgacat ccatcccccc cgggaaagca ctcttaagca gacagcctgg gcactccggt
     1981 tctttccgga cagtcctgca acaccgatga acaggacagg gctgagaaac gatacgatac
     2041 gacgatggaa cgctgactca cgagcccgtc gggcatgacg ggcaacataa ctgacccgcc
     2101 cgtcggataa cccgacgtcg gaagaggtta acacgcgcgg tatttccggg ttcgccactg
     2161 aatcgtaata aatgatctct cgcccctgca taaccgctct ccatcaacac cactcgtata
     2221 gcgcccgggc aacaccggta tgtaggatga cggggccgcg ttctaaagcc cgcatctctc
     2281 tcgtacctcg tcactctcgg aaaggaactg gcaggtcccc tcgcacttta cgagcccttt
     2341 ctccaggaca tatcccctgt cggacatctt aagcgcgagc gccgccttct gttcgacgag
     2401 cagaagggtc atcttctccg tctcgcagag cttcttgatt atgtctatga gttcccatat
     2461 gatgaccggc gccagcccct caaagggctc gtcgagaagg acaagcttgg ggtcgcacat
     2521 gaggcccctc gcgatggcaa gcatctgctg ctggcctccg gaaaggtacc ctccgccctg
     2581 ggattccttt tccttcagga tgggaaagta catgtacgcc ttctccagca tctcctcttt
     2641 tcgggccttc gtcatgtcag gagcgttgct cgtgcctatc aaaaggttct cctttaccga
     2701 gagccg
//
LOCUS       DAOQTR010000312         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_45231_length_2689_cov_3.302202, whole genome shotgun sequence.
ACCESSION   DAOQTR010000312 DAOQTR010000000
VERSION     DAOQTR010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45231_length_2689_cov_3.302202"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKN85_07820"
     CDS             complement(<1..420)
                     /locus_tag="PKN85_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT44356.1"
                     /translation="MDAPPLIEFRNVTKRFNGKTVLDNVNLSIYENQVTTIIGKSGTG
                     KSVLLKHIIGLLKPDEGTILFRGTPINKMKKAEWEGHRSKIAYLFQNNALLDSMTVLE
                     NVAFPLRQTTDLPKAEIEKRAMKRIEDLELAEAVGKFP"
     gene            complement(430..1251)
                     /locus_tag="PKN85_07825"
     CDS             complement(430..1251)
                     /locus_tag="PKN85_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT44357.1"
                     /translation="MKERDETNKPVNMLVSFFSTVGRWTIRTVGNFGAMAVFFFLGFI
                     NIFRSRQIREITSQTYYIGAKSSLIVMLVALFTGMVLGLQLFYTLVKFGSVGALGSAI
                     SLSLVRELGPVLTAIMITARAGSAMAAEIGILRNSEQIDALYTMGVDPIRYLISPKIA
                     ASIISFPLLTAFFDLIGIIGGYITGVVLLGTNGGTYFYRVQSSIDMVDIRGGFIKALV
                     FGAIVSCICCFQGYFCHMRSDGFGAKSVGLATVSAVVISCVMILVSDYVVTSFLM"
     gene            1572..1916
                     /locus_tag="PKN85_07830"
     CDS             1572..1916
                     /locus_tag="PKN85_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44358.1"
                     /translation="MDVKRCTVVAVLLAASILLSGCGKPVPPDKAAYVGEWQSREMYL
                     SIAEDGSVKYKRMSGGVSKSLSAPLRGFEGDNFVVGLPFLSTTFIVARPPYEADGQRK
                     MIVDGVTLTRFR"
     gene            complement(1933..>2689)
                     /locus_tag="PKN85_07835"
     CDS             complement(1933..>2689)
                     /locus_tag="PKN85_07835"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HNT44359.1"
                     /translation="QFGAFELGRLARPGRFYDFTRYRPTIDIEGAINELAVPNTTVHY
                     ARRQGQNDLVFLDLLEPHTNADVYVESVVRLLKALRTSKYVLLGSMYDVVPHTRPLLV
                     SGYGMGEKALQDVRMAGAMPITYRGPSTFANLITKKAAEEGIDAVVFIVSLPQYVVME
                     EDHVGKVRLMEVLNTLYNIPIDKEEFEKALRQREIITERLKASPEVADVLSQLENTYD
                     MRVKAMEKEGILQLGSDMEEIFWKTMEKNIGKA"
BASE COUNT          565 a          804 c          747 g          573 t
ORIGIN      
        1 cgggaatttc ccgacagcct ccgcaagttc cagatcctct atccgcttca tcgcccgctt
       61 ctctatctcc gccttcggca ggtccgtcgt ctgacgcagg ggaaaggcca cgttctcgag
      121 gacggtcatc gaatcgagga gggcgttgtt ctggaacagg taggctatct tgctcctgtg
      181 accttcccac tcggccttct tcattttatt gatgggcgtg ccccggaaga ggatggtgcc
      241 ttcgtcgggc ttgagcaggc cgatgatgtg cttgaggagc acgctcttgc cggtaccgct
      301 cttcccgatg atcgttgtga cctggttctc atagatgctc aaattgacgt tgtccagcac
      361 ggtctttcca ttgaaccgct tcgtaacgtt cctgaactct ataagagggg gggcatccat
      421 atctcgtcct tacatcagga atgatgtcac cacgtagtcg gaaacgagga tcatcacgca
      481 ggagatcacg accgccgata ccgtggccaa acctaccgat ttcgcgccga acccgtcact
      541 cctcatgtgg cagaaatatc cctggaagca gcagatgcag gagacgatgg cgccgaacac
      601 gagggccttt atgaaccccc cccggatatc caccatgtcg atggaggact ggacgcggta
      661 gaaatatgtg cccccattcg tccccaggag gacgacgccc gtgatatacc cgccgatgat
      721 gcctatgagg tcgaaaaatg ccgtaagcag gggaaagctg atgatcgaag ccgctatctt
      781 cgggctgatc aggtagcgga tggggtccac acccatcgtg taaagcgcgt cgatctgctc
      841 tgaattacgc agtatgccga tctcggcggc catcgccgaa ccggcacgcg ccgttatcat
      901 gatggccgtc aggaccggcc caagctcccg aacgagggaa agggaaatgg ccgacccgag
      961 ggcgcccacg gaaccgaact tcacgagggt gtagaagagc tgaagaccca gcaccatgcc
     1021 cgtgaaaagg gcgacgagca tcacgatgag ggaggatttt gcaccgatgt agtaggtctg
     1081 gctcgtgatc tcacggatct gcctcgacct gaagatgttg atgaaaccga ggaagaagaa
     1141 gacggccatg gcgccgaaat tcccgacggt acggatggtc caccggccta cggtcgagaa
     1201 aaaagagaca agcatattca caggtttgtt tgtctcgtcg cgttccttca tgtcgtcccc
     1261 agcatcccaa gccgaaaatg cctgccctct ctcccggttc catgacgacc gggccaaaca
     1321 ttgcgacagg ccgatacctc ttatctccgt tcagccatta taagcaacgg gacccgtact
     1381 gtcaactaat gcgtaagcat ttcaagacca tgtccaacgt ccttttgtca aggttttcat
     1441 cacgggaccg gggtggacca ggccgcctgg cgcgctgtcg acccaacgga cctgttgcct
     1501 tttccgccgt cacggagtag catatcggcg atgagcacag gagcggacgt gatcggaaga
     1561 gaggcggtcc catggatgtg aagcggtgca cggtggttgc cgtgctcctt gcggcatcca
     1621 tccttctatc cggatgcggc aagccggtac cacccgacaa ggccgcgtac gtcggcgaat
     1681 ggcaatcacg ggagatgtac ctctccatag ccgaagatgg gtccgtgaag tacaaacgca
     1741 tgagcggagg cgtctcgaaa tccctgtccg cgccgctacg gggctttgaa ggggacaact
     1801 tcgtcgtcgg ccttcccttc ctttcgacga cattcatcgt cgccagaccg ccctatgaag
     1861 ccgacggtca gcggaagatg atagtggacg gcgttaccct gacccgcttc cggtagcggc
     1921 ccttggacgg cgtcaggcct tccctatgtt tttttccatg gtcttccaga agatctcttc
     1981 catgtcggag ccaagctgga gaatgccttc cttctccatc gccttgaccc gcatgtcgta
     2041 ggtgttctcg agctgcgaga ggacgtccgc cacctccggg gaggccttga gcctctccgt
     2101 gattatctcg cgctgccgca gcgccttctc gaactcctcc ttgtcgatgg gaatgttata
     2161 gagcgtgttg aggacttcca tcaatctgac cttccctacg tggtcctctt ccatcacgac
     2221 atactggggc agggaaacga taaagaccac cgcgtcgata ccctcctccg cggccttttt
     2281 cgttatcagg ttggcaaagg tcgaagggcc cctgtaggtg atgggcatgg cgcccgccat
     2341 cctcacatcc tggagcgcct tttcgcccat cccgtatcca ctcacgagca acggtctcgt
     2401 atggggcacc acatcgtaca tgcttcccag caggacatat ttgctcgtcc tgagagcctt
     2461 aagaagtctc acgacggact cgacgtagac atcggcattg gtatgaggct ccaggagatc
     2521 gaggaaaaca aggtcattct gcccctgcct ccgggcatag tggaccgtcg tgttcggcac
     2581 ggccagctcg tttatggctc cctcgatatc tatcgtaggc cggtacctgg taaaatcgta
     2641 gaaccgcccc ggcctcgcga gccggcccag ctcgaaggcc ccgaactga
//
LOCUS       DAOQTR010000313         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_45518_length_2678_cov_4.314525, whole genome shotgun sequence.
ACCESSION   DAOQTR010000313 DAOQTR010000000
VERSION     DAOQTR010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45518_length_2678_cov_4.314525"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="PKN85_07840"
     CDS             <1..1453
                     /locus_tag="PKN85_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNT44360.1"
                     /translation="HRASAKDASSNISTKTCDVNDDAPHIIVPLPGDLSWGIIDIYNN
                     TTGSGTMRQVRHYRIFVAVALAAFILASARGYAMNLIFEDAQYSFQTLRATAATAGGA
                     ADIGECLKTAYRITEGDDESWYREWFRTAEQRRKEADDLAAQGRMMSAHQEYLKASNY
                     YRTAEFFLHNNPEDPRILATWREGRESFLKAARLSRAPIIPVEIPFEGTTLPGYRCLV
                     DDRKVKRPLLVIQTGFDGTAEELYYTVAVFALARGYNCLLFEGPGQGRVIREQKIPFR
                     PNWETVITPVVDFALGQEETDPRHIAIMGISFGGYFVPRAMAFEKRIPFCIVNGGVYD
                     FHRTARLTPELEKMLDSAEGSGAIDREVYERMEKSPSLRWAVGNGMFTFHAKTPSEWF
                     RMTRAYTLEGVTDRITCTMLVVDSEDDRDMPGQSRMLYDALRSPRDYMLFTREEGAEE
                     HCQVGASRISNARILDWLDNAMKAKPMMEGSRE"
     gene            complement(1562..2581)
                     /locus_tag="PKN85_07845"
     CDS             complement(1562..2581)
                     /locus_tag="PKN85_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT44361.1"
                     /translation="MGERTYPLRMVAWELTRNCNLSCMHCRASAASGPYEGELSTEEC
                     KAVIDDILSFSEPTVILTGGEPLMRRDIFDIIEYGNDRGLRMVIAVNGTLVDEGVAKR
                     LEASGIKRVSLSIDARDGTGHDKFRGVDGSFRAVTGAAGTLKLTGLPFQINTTVTRLN
                     VDDLDDIYALVRSLGAVAWHVFLLVPVGRGTGLKGKELSAEAYEEVLHRLRAIEKRGE
                     LEMKVTCAPHYYRIVKEDGETPKSAGCLAGKSFMFISHRGIAQPCGYLEIDSGDVRRD
                     GVRTVWEGSEVFRRLRDLTLYRGKCSTCRYVRICGGCRARAYEETGDYLQEEPLCSYR
                     EKNNQ"
BASE COUNT          570 a          803 c          773 g          532 t
ORIGIN      
        1 ccatcgtgca agcgctaaag acgcatcaag caatatcagc acgaaaacgt gcgatgtcaa
       61 cgacgatgcg ccgcacatca ttgttccctt gcctggtgac ctttcctggg gcataataga
      121 catatacaac aacacgacgg ggagcggcac catgagacag gtcagacatt accggatatt
      181 cgttgcagtt gcccttgcgg cgttcatatt ggcatctgcc agggggtatg ccatgaacct
      241 tatcttcgag gacgcgcagt actctttcca gaccttgagg gcaacagcgg cgacggctgg
      301 gggcgccgcc gacatcggtg aatgcctcaa aaccgcctac cgcatcacgg aaggcgatga
      361 cgagagctgg taccgcgaat ggttcaggac ggccgagcag cgaaggaagg aagccgatga
      421 tctcgccgca cagggcagaa tgatgagcgc acaccaggaa tacctgaagg cgtccaacta
      481 ttacaggacc gccgagttct tcctccacaa caaccctgaa gacccccgaa tacttgccac
      541 ctggcgagaa ggccgcgaga gctttctcaa ggccgcgcga ctttcccggg caccgataat
      601 tcccgtggag atccctttcg aaggcacgac cctcccgggg tatcgctgtc tcgtggatga
      661 ccggaaggta aagcgaccgc ttctcgtcat ccagacaggt ttcgacggaa ccgcggagga
      721 gctctattat accgtggccg tattcgccct cgcgagagga tacaactgtc tgctgttcga
      781 ggggccgggt cagggacgcg tcattcgtga acagaagata ccattccgcc cgaactggga
      841 aaccgtgatc acacctgttg ttgatttcgc tctgggacag gaggaaacgg acccccggca
      901 catcgcgata atggggatca gcttcggggg gtatttcgtg ccgcgggcca tggccttcga
      961 gaaaaggatc cccttctgca tagtgaacgg cggcgtctac gactttcaca ggactgcccg
     1021 cctcaccccc gagctggaga agatgctcga ctcggcggag ggctccgggg ccatcgacag
     1081 ggaggtttac gagaggatgg aaaagagtcc ttccctccga tgggcggtcg gcaacggcat
     1141 gttcaccttc cacgcgaaga cgccgtcgga gtggttcagg atgacacggg cctacacgct
     1201 tgaaggggtg acagacagga tcacctgcac gatgctcgtc gtcgactccg aggatgacag
     1261 ggacatgccg ggccagtcac ggatgctgta cgacgcgctc aggtctcccc gggattacat
     1321 gctcttcacc agggaagaag gggcggagga acactgccag gtgggcgcct cccgtatatc
     1381 gaacgcgcgc atcctcgact ggctggacaa cgcaatgaag gcaaaaccga tgatggaggg
     1441 aagcagggaa tgaaggctca atgaacaact gcaccgtatc ctgccggatg ggagctttga
     1501 cgcttttcgt tcgactgtcg atcttcggcc aattcgctct tgtctggtta ttgggatttg
     1561 atcattggtt attcttttcc ctgtacgaac acagcggctc ttcctggagg taatctcccg
     1621 tctcctcgta ggcgcgggcg cggcagccgc cgcagatgcg gacatagcgg caggtcgagc
     1681 attttccacg atataacgtg agatcgcgca ggcgccggaa cacctcggag ccctcccaga
     1741 cagtcctcac accgtccctt cgcacatcgc ccgaatcgat ctcaaggtaa ccgcagggct
     1801 gggcgatgcc tcggtgagag atgaacatga agctcttccc ggcaaggcac cctgcgcttt
     1861 tcggcgtctc cccatcttcc ttcacgatgc ggtaatagtg cggggcacag gtcaccttca
     1921 tctccagctc gcccctcttt tcaattgccc gaagccggtg gagcacctcc tcataggcct
     1981 cggcggagag ttcctttccc ttgaggcctg ttcccctgcc gacaggcacg aggagaaaga
     2041 cgtgccaggc gacagccccg agggacctga ccaacgcata gatatcgtcg aggtcatcga
     2101 cattgagcct cgtcaccgtg gtatttatct ggaaaggcag acccgtcagc ttgagggtcc
     2161 ctgccgcccc ggtcacggcc cggaaggacc cgtcgacacc gcgaaacttg tcgtgaccgg
     2221 ttccgtccct tgcgtcgatg ctgaggctca ccctctttat acccgaggcc tcgagccttt
     2281 tcgccacccc ttcgtcgaca agggtaccgt tcacggcgat gaccattctc aatcccctgt
     2341 cattgccata ttcgatgatg tcgaagatat cgcgtctcat cagcggttcc ccgccggtca
     2401 aaatgaccgt cggctccgag aaggacagga tgtcatcaat gacggccttg cactcttccg
     2461 ttgagagctc gccctcgtag ggacccgacg ccgctgacgc gcggcagtgc atgcaggaaa
     2521 ggttgcagtt gcgggtcagc tcccaggcga ccatcctcaa agggtatgtt ctttcgccca
     2581 tgtaccgatc tctttcgcaa agtaggtaat gatgatgtcc gcgccggcgc gccggatgct
     2641 tgtcgtggat tcgatgaccg cccgcttcag atcgaagg
//
LOCUS       DAOQTR010000314         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_45523_length_2678_cov_3.310332, whole genome shotgun sequence.
ACCESSION   DAOQTR010000314 DAOQTR010000000
VERSION     DAOQTR010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45523_length_2678_cov_3.310332"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..208)
                     /locus_tag="PKN85_07850"
     CDS             complement(2..208)
                     /locus_tag="PKN85_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44362.1"
                     /translation="MQSTMLEGSSLTVPNDLGYLPAIQAFVSEVMERRGYSKRDTTMF
                     LIALEEAIVNVVKHGYDHNQPLMW"
     gene            516..>2678
                     /locus_tag="PKN85_07855"
     CDS             516..>2678
                     /locus_tag="PKN85_07855"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNT44363.1"
                     /translation="MDREDADAQDVKLLSAEDVRTGLDADTMRRAILENLNFIQARIP
                     VLATRNDWYMALAYTVRDRMLENWVRSTEGLRRTPHAVAYLSAEFLMGPHLGNAMVNL
                     GIREHTAEAVAALGLDLDELLEQEAEPGLGNGGLGRLAACFLDSLATEKIHALGYGIR
                     YEFGIFDQEIVDGWQREITDKWLALGNPWEIARPEITYYVQFGGHTQQYQDEDGRYRV
                     RWVPERVVKGMAYDTPIPGYRVEIVNILRLWKSEACESFDFQAFNTGDYDGAVEEKIA
                     SEMISKVLYPNDEAAAGKRLRLAQQNFFVSCSLQDMIRIHAFVGGKPETLHERFAVQL
                     NDTHPSIAVAELMRLLVDQYRIDWDTAWDATRRTFSYTNHTLLPEALEKWPVPLFQNL
                     LPRHLEIIYEINRRFLDEVRAAYPGDEERARRLSLIDETGDRYVRMAHLATVGSHTVN
                     GVAVLHTELLKRTVMRDFHELTPEKFRNVTNGVTPRRWMVLSNPAMSKLITDAIGDGW
                     VCDCQNNIRRLEPFAGDAGFREAWSEAKRRNKSFLARLIRERTGVVVDPESMFDIQVK
                     RIHEYKRQHLNALHVVSHYLRLKRGHKAEVAPRTVIFGGKAAPGYYMAKLIIKLITSI
                     AEVVNGDPETREKLKVVFLPDFNVKLGQRVYPAADLSEQISTAGKEASGTGNMKFSMN
                     GALTIGTLDGANVEIREEVGAENFFLFGLDAGEVFDLKS"
BASE COUNT          580 a          758 c          810 g          530 t
ORIGIN      
        1 attaccacat caggggctgg ttatggtcgt atccgtgttt caccacgttc acgatggcct
       61 cttcgagggc gatgaggaac attgtcgtat cccgtttact gtagccgcgg cgctccatca
      121 cctccgatac gaaggcctgg atcgccggaa gatacccgag atcgttggga acggtaaggc
      181 tggagccttc aagcatcgtt gattgcatga tcatcccctg ttagatagtc gcgaacccga
      241 tagatcaagt gagagtcatt atactccaca gagtctccca tcttcatcaa aaaaaacgcc
      301 gcatggtacc aacaaggcgc atccctccgg accacaccct tcattttccc tccagtcccg
      361 aagccattca agcgatttga cctgcctctc ccgtttttcg cctttgaacg ttcaagcagc
      421 tcaagctgtt caagcggctc cagccgtccg tattgacacc accacgagaa atcgggttac
      481 tatgttgata gcggtgtaga ccagaggagg ttgccatgga tcgggaagac gccgatgctc
      541 aggatgtgaa gcttctgtca gcggaagatg tccggacggg tctcgacgcc gacaccatga
      601 ggcgcgcaat tctggagaat ttgaatttca tccaggcccg cattccggtg ctggcgacca
      661 ggaacgactg gtacatggcc cttgcgtaca cggtgcggga caggatgctg gagaattggg
      721 tccgttcgac ggaagggttg cgccgcactc cccacgcggt ggcctatctc tcggcggagt
      781 tcctcatggg cccccacctg ggaaacgcca tggtcaacct ggggatccgg gagcacacgg
      841 cagaggcggt ggccgcgctc ggtctcgatc tcgacgaact tctggaacag gaggctgagc
      901 cgggtcttgg aaacgggggg ctggggaggc ttgccgcctg tttcctcgat tccctggcga
      961 cggagaagat acacgccctc ggttacggga tacgctacga attcggcatc ttcgaccagg
     1021 agatcgtgga cggctggcag cgggagataa cggacaagtg gctggccctc ggcaatccct
     1081 gggagatcgc ccggcccgag atcacttact acgtgcaatt cgggggtcac acccagcaat
     1141 accaggacga ggatggacgc tatcgggtcc gctgggttcc cgaaagggtt gtgaagggta
     1201 tggcgtatga tacgcccatt ccgggatatc gtgtcgagat agtcaacatc cttcgtctgt
     1261 ggaagtcgga agcctgcgaa tcgttcgatt ttcaggcctt caatacgggc gattacgacg
     1321 gggctgtcga ggagaagatc gcctccgaaa tgatctccaa ggttctttat cccaacgacg
     1381 aggcggcagc gggcaagagg cttaggctgg cgcagcagaa tttcttcgtt tcatgctccc
     1441 tgcaggacat gatccgcatc cacgccttcg tgggggggaa gcccgagacc cttcatgaga
     1501 ggtttgccgt tcagctcaac gacacccatc cgtccatagc cgtcgcggaa ctcatgcggc
     1561 ttctcgtcga ccagtaccgc attgactggg atacggcatg ggacgcgacg agaaggacct
     1621 tctcgtacac gaaccacacg cttcttccgg aagccctcga gaagtggccg gtaccgctct
     1681 tccagaacct gctgccccgg cacctcgaga tcatatacga gatcaacaga cgctttctgg
     1741 acgaagtcag ggcggcctat ccgggagatg aggagcgggc gcgccgcctg tcgcttatag
     1801 atgagaccgg ggacaggtat gtgcgcatgg cgcacctcgc caccgtggga agccacacgg
     1861 tgaacggagt ggcggtgctt cacacggaac tcctgaagag gacggtgatg cgggactttc
     1921 acgaactgac accggaaaag ttccgcaacg tcacgaacgg ggtgacgccc cgtcgatgga
     1981 tggtgctgtc gaatcccgcc atgtcgaagc ttatcaccga tgccatcggc gacggctggg
     2041 tctgcgattg tcagaacaac atccgacgcc tcgaaccatt cgccggtgac gccggtttcc
     2101 gggaagcctg gtcagaggca aaacgccgga ataagagttt cctggcgcgg ctcatcaggg
     2161 agcggacggg ggtagtcgtg gatccggaga gtatgttcga tatccaggtc aagcggatcc
     2221 acgagtacaa acggcagcat ctcaacgcgc tccacgttgt ctctcattac ctcaggctca
     2281 agaggggtca taaagctgaa gtcgcccccc gcaccgtcat attcggcggc aaggccgcgc
     2341 cgggctatta catggcaaaa ctcatcataa agctgatcac ctccatcgcg gaggtggtga
     2401 acggtgatcc cgagacgcgc gagaagctca aggtggtgtt ccttcccgat ttcaacgtca
     2461 aactcggcca gcgcgtatac ccggcggcag acctgtcgga gcagatctcc accgcgggca
     2521 aggaggcatc gggtacgggg aacatgaagt tttccatgaa cggcgccctg acgataggga
     2581 ccctcgacgg ggcgaacgtc gagatacggg aggaggtggg ggcggagaat ttcttcctct
     2641 tcggcctcga tgccggagag gtctttgatc tgaagtcg
//
LOCUS       DAOQTR010000315         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_45653_length_2673_cov_4.150879, whole genome shotgun sequence.
ACCESSION   DAOQTR010000315 DAOQTR010000000
VERSION     DAOQTR010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45653_length_2673_cov_4.150879"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKN85_07860"
     CDS             <1..716
                     /locus_tag="PKN85_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019292901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44364.1"
                     /translation="SMLGLAELLERKPRELSGGQRQRVALGRALVRSPKVFLMDEPLS
                     NLDARLRLETRGELKRLHKQLGTTTVYVTHDQAEALSLSDRIAVLDRGDIRQVGTPRE
                     VYSDPANTFVAGFMGSPSMNFIAAVVRRREPLTVECNGLILSPAVKRTPEEEHVLIGV
                     RPEDISISKRVNGEGIAVSVLLVEPAGPFHWADVAWGPAVLRGMAEAQEELAPGDWAH
                     MAFHPDEALIFEAASGLRV"
     gene            878..1789
                     /locus_tag="PKN85_07865"
     CDS             878..1789
                     /locus_tag="PKN85_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT44365.1"
                     /translation="MPQIPPLYLEILQKLAISIIIACLAVVVSRVAARLLRKRVKDGS
                     EMQTLGVLVRKITYITAVVIILVVVFELGSNFGTVLGILGAGVAFASQEVIGSFAGYL
                     NIITGSIFRIGDRIRIGNVVGDVLDISLLRTTVMEIGEWVKADQYTGRIVSLANRVVF
                     SDPVFNYTQYSRYLWDEIMVPVTYDTNWPRAVEIILAKGQELTLGFHAEARAEFEEMA
                     KKYPSLPAVPVEPAVYIAMTDNWVELTLRYIVEARERRSIKGQIHRELLTSFGEETEI
                     TVASMTVDIVKFPPLRSNDPAPKAPVP"
     gene            complement(1842..2105)
                     /locus_tag="PKN85_07870"
     CDS             complement(1842..2105)
                     /locus_tag="PKN85_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44366.1"
                     /translation="MKSARGRLWRACLIASVILGGSTAAGSAGETTKIYDRNWNIKGY
                     IRDDGGTTKKIYDKNRNLQGYIKEDRIYDRNWGRKGTLRDPNR"
     gene            complement(2144..2311)
                     /locus_tag="PKN85_07875"
     CDS             complement(2144..2311)
                     /locus_tag="PKN85_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44367.1"
                     /translation="MVMIDAGRCDGGEGVACDWCGKEEAVGVFFVPGRGVLSLCAHCR
                     VFLVAVLIDVA"
     gene            complement(2407..>2673)
                     /locus_tag="PKN85_07880"
     CDS             complement(2407..>2673)
                     /locus_tag="PKN85_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNT44368.1"
                     /translation="AFIDGDPVDVVLRFDETYRPLILRKTWHPSQRHLELPDGRIEMT
                     FRVNGFLGIGKWIHQWIPHVEVVTPKELRDAFLDELKNAVEKHG"
BASE COUNT          541 a          764 c          802 g          566 t
ORIGIN      
        1 cgtcgatgct gggtctcgcg gagcttcttg aacgaaaacc ccgggagctt tcgggtggac
       61 agaggcagcg cgtcgccctg gggagagccc tcgtcaggtc gccgaaggtc ttcctgatgg
      121 atgagcccct ctccaatctc gatgcccgcc tgaggctgga aaccagggga gagctgaaga
      181 gactgcacaa gcagctggga acgacgaccg tctacgtcac gcatgaccag gccgaggcgc
      241 tcagtctctc agacaggatt gcggtgctgg accgcggcga catccgccag gttgggacgc
      301 cccgggaggt gtattcggac ccggccaaca ccttcgtggc cgggttcatg ggcagccctt
      361 cgatgaactt catcgccgct gttgtgagac ggagggagcc gctcacggtc gagtgcaacg
      421 gcctcatcct gtctcccgcg gtgaaaagga cgccggaaga ggaacatgtc ctgatcggcg
      481 tgaggccgga ggacatctcc atatcgaaaa gggtgaatgg tgaggggata gcggtcagtg
      541 tcctgctcgt cgagcccgcc ggtccctttc actgggcgga tgtggcatgg ggcccggcgg
      601 tcctgagggg tatggccgag gcgcaggagg agcttgcgcc aggcgactgg gcacacatgg
      661 cctttcatcc cgatgaggcc cttatcttcg aggcggcatc gggtctgagg gtctgaggcg
      721 gggatgccgg ttctcaccag gccctgtgtc cgggacatat ttccgataat atcttgcaaa
      781 ttatttgaca ttacaggaga attgaaaagg cgtcggctga aagagaagcc agccgggcaa
      841 atggatcgaa ggatttgaga cctcagggag gttgatcgtg ccgcagattc caccgctcta
      901 ccttgagata ctgcagaagc tggccatttc gatcatcatc gcctgcctcg ccgttgtcgt
      961 cagccgcgtt gccgcgagac tgctgagaaa acgcgttaaa gacggctctg aaatgcagac
     1021 attgggtgtg ctcgtccgca agatcaccta cattacggcc gtcgtgatca ttctcgtggt
     1081 tgtctttgag ctgggcagca acttcggcac cgttctgggt atcctggggg ccggcgtggc
     1141 ctttgcctca caggaggtga taggctcctt cgctggctac ctgaacataa tcaccgggag
     1201 catattcagg atcggcgaca ggatacggat aggcaatgtc gtcggtgacg ttctcgacat
     1261 cagcctgttg cgaacgaccg tcatggagat aggcgaatgg gtgaaagctg accagtacac
     1321 gggcaggata gtgagcctcg ccaaccgggt ggtcttctcc gaccctgtct tcaactacac
     1381 ccagtattcg cgatacctct gggacgagat aatggtcccc gtcacctatg atacgaactg
     1441 gccccgtgcc gttgagatca tactcgcgaa ggggcaggag ctgacgctgg ggttccatgc
     1501 cgaagcgaga gcggaattcg aggagatggc aaaaaagtat ccctcactcc ccgccgttcc
     1561 tgtcgagcca gccgtctaca ttgccatgac ggacaactgg gtcgagctga cattgcgcta
     1621 catcgttgaa gcgagggagc gcagatcgat aaaaggacag atccacagag aactcctcac
     1681 gtcctttggc gaagagacgg agatcaccgt ggcctctatg acggttgata tcgttaagtt
     1741 cccaccgttg agatcgaacg atcccgcccc gaaggccccg gtcccttgag acggatcgga
     1801 gcctcgcggg ttatccatgc accagtgtct atgcaaggtc ttcatcggtt ggggtcccgg
     1861 agcgttcctt tgcggcccca gttcctgtcg tagatacggt cctccttgat gtagccctga
     1921 aggttccgat ttttgtcata gatcttcttt gtcgttcctc cgtcgtcccg gatatagccc
     1981 ttgatgttcc agttcctgtc gtatatcttg gtcgtttccc ctgcggatcc cgcagcggtg
     2041 gacccaccaa ggatcaccga cgctatcagg cacgccctcc acagcctgcc gcgagccgac
     2101 ttcatgttgt tcacctccca accacggaaa gtcctttccg tcgtcaagcg acgtctatga
     2161 ggacagccac gaggaatacc cggcagtgag cgcaaagact gagcacgccc ctgcccggca
     2221 caaagaatac gcccacggct tcctccttcc cgcaccagtc acacgcaacg ccctcaccac
     2281 catcacatct ccccgcatcg atcatgacca tcacccctct tgcttataat ttaatgatgt
     2341 ggtgtgacag cagtgtgtca gaagggagag gaccaagaga agggcgctgc gaggcgtgta
     2401 ttatttctac ccgtgcttct ccacggcgtt cttgagctcg tccaggaagg cgtcgcgcag
     2461 ttctttcggg gtgacgacct cgacgtgggg gatccattgg tggatccact tgccgatccc
     2521 caggaagccg ttcaccctga aggtcatttc gatacggccg tcggggagtt cgaggtgccg
     2581 ctggcttggg tgccaggttt ttctcaggat gaggggcctg taggtttcat cgaatctgag
     2641 gaccacgtcg acgggatcgc cgtcgatgaa ggc
//
LOCUS       DAOQTR010000316         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_45779_length_2669_cov_3.529074, whole genome shotgun sequence.
ACCESSION   DAOQTR010000316 DAOQTR010000000
VERSION     DAOQTR010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45779_length_2669_cov_3.529074"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..141
                     /locus_tag="PKN85_07885"
     tRNA            66..141
                     /locus_tag="PKN85_07885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Lys,seq:ctt)
     gene            complement(463..1083)
                     /locus_tag="PKN85_07890"
     CDS             complement(463..1083)
                     /locus_tag="PKN85_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44369.1"
                     /translation="MEKKIDTADTDRLKQALQPYLPGNIITDMFEDRVLTDLWDVLYT
                     AVEYYTGGEKDALEAVRKEKVESVDQLLDRLRWWGKSAESPLDIERIKELLEPYLPED
                     VIDEALLDGESGDLKDLLTIAVDYFTDGDRRAMDSIKSVEISGVDDLLDQIRWWYNAT
                     ESPDAWYDPVEGKWYSPDENYEEDYGDSDYGGEDLSVRDEEEGEVE"
     gene            complement(1392..2024)
                     /locus_tag="PKN85_07895"
     CDS             complement(1392..2024)
                     /locus_tag="PKN85_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44370.1"
                     /translation="MTDSASQALSILRDTSNFEWYVIPFLLVVIYIYHNEIRLKNYSA
                     VFAGLALWGCDWFNEIWNALVFHFTQYAPVWGTPGDSAYVILIGLNIEISLMFLLMGV
                     ACTIILPEDRRMKILGIPNRLFFAIIFTTLAVIVEIILNAIGALTWEYSWWSARFPWL
                     LWIFGYFYFFVVAYLVYDMRTIRAKAITVGAIFAVDIASLVIFMGVLGWI"
     gene            2347..>2669
                     /locus_tag="PKN85_07900"
     CDS             2347..>2669
                     /locus_tag="PKN85_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT44371.1"
                     /translation="MNREEKLTRIFEAALRVFARYGYRRTRVEDIADELGMTKGNLYL
                     YVKDKRDLYEKAVAQGLLRWQAMVGRSIEKIDDVCDQFLTMGRKSYAYLSRDVSLRTV
                     LINDPS"
BASE COUNT          589 a          762 c          739 g          579 t
ORIGIN      
        1 gcgtcggact tcgaatccgc aggtcgggcg ttcgagtcgc cccgggcgta ccagaaacag
       61 catatgggcc attagctcaa caggtagagc aactgactct taatcagtag gtcccaggtt
      121 cgagccctgg atggcccacc aaaggaagag gggcgcggca gatgacgccg ggtccctctt
      181 ccttctttgt attcgggccg cacggccgcc gatgcgggcc cactgaagga gagaggcccc
      241 tttcccgttg caaggacggt aacacatggt tctcaggagt cggttttcca ccttcccgaa
      301 tatcctgatg atcacggaag caatcgagat caggggcggg aacggcaggc agcgggctcc
      361 acgcagggtc ggaccccgcc ttcaggggct cgtccttctt tgcgctccgc caggattccc
      421 tccccgcgca cgtgcctgcg gcatggcaag aggacatgcc gctcactcca cttccccttc
      481 ttcctcgtct ctcacgctta agtcttctcc gccgtaatcg ctgtcgccgt agtcttcctc
      541 gtaattctca tccgggctgt accatttccc ttccaccggg tcgtaccatg catccggaga
      601 ttccgtggca ttgtaccacc acctgatctg gtccagaagg tcatccacgc cggaaatctc
      661 gacactcttg atcgagtcca tggcgcgcct gtcgccgtcg gtaaagtagt ctacggcaat
      721 ggtaaggagg tccttcaggt ctccgctctc tccgtccaga agcgcctcgt cgatgacgtc
      781 ttcgggcaga tagggctcca acagctcctt gatcctttcg atgtccagcg ggctttccgc
      841 gcttttgccc caccacctga gcctgtcgag aagctgatca acgctttcga ccttttcctt
      901 cctgacggcc tccagagcat ctttttcacc gccggtataa tattccacgg cggtgtacag
      961 cacatcccac aggtcggtga gaaccctgtc ttcgaacata tcggtaatga tattgccggg
     1021 gaggtagggt tgcagggcct gcttcagtct gtccgtatcc gctgtatcaa tcttcttctc
     1081 catggggcgt cctccctgtt ctccgtgtcg gggcacaggg cgggcccgtt tcagcgaacc
     1141 ctgcggcaat gcctccgcct gtgacaatcc cgggcggaga acggtgttgt taatagttat
     1201 atggagtgtc gatcacggtt attcaatatc cgaaaaactg gggtggggct cgatggaagg
     1261 cggccggaga attcttccgg tacggccgtg aaaaaatgtc cgccgtgcca gaaagtccag
     1321 tcggagacga ggcgggttca ggacagacag ggccgggaaa cccccggccc tgctcacgat
     1381 cgtctcacgc gctatatcca gccgagcaca cccatgaaga tcacgaggct cgcgatgtcc
     1441 acggcgaaaa tcgctcccac ggtgatggcc tttgcccgga tggttctcat gtcgtagacc
     1501 agataggcga ccacgaagaa gtagaagtat ccaaagatcc acagcagcca ggggaaccgc
     1561 gcgctccacc aggaatactc ccaggtcaac gcgcctatgg cattgaggat gatctccacg
     1621 atcacggcca gggtggtgaa tatgatggcg aaaaaaagcc ggttggggat cccgaggatc
     1681 ttcatcctcc ggtcctcggg cagaatgatg gtgcaggcga cgcccatcag gagaaacatc
     1741 agggagatct cgatattcag cccgatgaga atgacatatg cgctgtcacc cggcgtcccc
     1801 cagacaggcg cgtactgggt gaaatggaac accagcgcgt tccagatctc gttgaaccag
     1861 tcacaacccc acagggcgag gccggcgaag accgcgctgt aattcttgag ccgtatttcg
     1921 ttgtgataga tgtagatgac gacaagcaag aacgggatga cgtaccactc gaagttcgaa
     1981 gtgtccctca ggatggatag tgcctgagat gccgaatctg tcataaaaca cctcctgcta
     2041 ttgtattgtc tgttccttta caccacagat gaaaacgctt gttgttctga ggcaatcgta
     2101 gtatccggca ggcggtcaag tcaatgaaaa tttgaacatt ttgttcactt catcatgttt
     2161 tcatctttcg cgtccgcccg gtgagcagca ggaaacctcc cctgcttcac ggatgctgca
     2221 tgaatcggca cgttatgacg gttgtgccgc ggacatccga tttcccgaag aatctctcaa
     2281 ggatgaattg ctattgacat cgaatacccg agatgcaaga tcagggatac acgaacatgg
     2341 tgctcgatga accgggaaga aaagcttacc agaatttttg aagccgcctt gagggtcttt
     2401 gcccgttacg ggtacagaag aacccgggtc gaagacattg ccgacgagct ggggatgacc
     2461 aagggaaacc tctacctgta cgtgaaggac aagcgggacc tctatgaaaa ggctgtagcc
     2521 caggggctgc tccgctggca ggcgatggtg gggagatcga tcgagaaaat cgatgacgtg
     2581 tgcgatcagt tcctcaccat gggcagaaag agctacgcct atctttcccg ggatgtatcg
     2641 ttaaggaccg tcctaatcaa cgatccgtc
//
LOCUS       DAOQTR010000317         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_45856_length_2666_cov_3.272309, whole genome shotgun sequence.
ACCESSION   DAOQTR010000317 DAOQTR010000000
VERSION     DAOQTR010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45856_length_2666_cov_3.272309"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1017
                     /locus_tag="PKN85_07905"
     CDS             145..1017
                     /locus_tag="PKN85_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT44372.1"
                     /translation="MRIDLHIHSKNGSDGRWDVRDIFAEASRRRLSVISVTDHDSVAA
                     QEQAQSLARKYGIRYITGIELNITFSHAGHKAGKEVSLDCLGYGFDYRHPPLTDKLER
                     LRSHRVRRAEQILEKLNEEFRAQGMPEFTGKDMDAIQANVDGALARPHIAAYLIEKGI
                     VRDKQEAFDRYLVKCDVPKMPLSLAEASALIRRAGGRLVLAHPGDPNGTSLVSLTPLV
                     EQQQDIIRDSMLEYIDGVECWHSRHEKAVVQSYADFAKRHSLMATGGSDCHQQPPVMG
                     TVEVPGWVAGQFDF"
     gene            1143..1745
                     /locus_tag="PKN85_07910"
     CDS             1143..1745
                     /locus_tag="PKN85_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44373.1"
                     /translation="MREPFKDFVEREIVDDAVLIACGLPATNKTETTEVIARIKGYAI
                     LRTDLIRRDVLKGQDIFDEKVASDMNKRNLVYEKMFSLADELASQGRGVILDATFIKQ
                     SLRKRAAAAAARNKRTFIIQQTSAPEAYSLNKISKRTKENYESNALTPEAYFNNKEKF
                     EPVDLDEIKRLYPDLSIVHLVVDTASDNEDGWYVISRETR"
     gene            1770..2549
                     /locus_tag="PKN85_07915"
     CDS             1770..2549
                     /locus_tag="PKN85_07915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT44374.1"
                     /translation="MKKIWIPVLILLLAAGCASTFKQKRELSKPMVAIAMGKIQQGEI
                     QGALVELRRAVQVNPKDPEVYYAYALAYWSTEDLDRALENIDKAVNLAGRLGLEHPGL
                     KSEAYNLKGTILVGMGDHDAAIKAFQNAVKDELYATPEYAYYNMASVYCSQKLYPEAE
                     QSAKEALQKNPHYAPAWKILGQSFMNQDKDAQAIEALQKAILEYPQYTEAHWEIAQVY
                     FKTGNTREAVKSLREVVILDENGLLGARARQSLQELGASVR"
BASE COUNT          645 a          761 c          779 g          481 t
ORIGIN      
        1 gttctacaag gtctatcggg cttacgtgcg ggccaaggtc acttccttca tgctcgacga
       61 cgccggcctc gacgggaccc gaaaacaggc cgcgctcgag accgcccgca ggtactacga
      121 cctcgcccac cggtatatca tgccatgagg atcgacctgc acatccattc aaaaaacggc
      181 tctgacggga gatgggacgt aagggacatc ttcgccgagg cctcacggcg gaggctctcc
      241 gtcatctcgg taaccgacca cgactccgtc gccgctcagg agcaggcgca gagcctcgcg
      301 cgaaaatacg gcatacggta catcaccggg atagagctca acatcacctt ttcccatgcc
      361 gggcataagg ccggcaaaga ggtgtcgctg gactgcctgg gctacggctt cgactaccgc
      421 catccccccc tgacggacaa actcgagcgg ctcaggagcc accgggtccg cagggccgag
      481 cagatcctgg aaaaactcaa tgaggaattc cgggcccagg gaatgcccga gttcacgggt
      541 aaggatatgg atgcgatcca ggcgaatgtc gacggagccc tcgccagacc gcatatcgct
      601 gcatatctca tcgaaaaggg catcgtgcgc gacaagcagg aggcgttcga ccgctatctg
      661 gtcaaatgcg atgtcccgaa gatgcctctg agccttgccg aggcttcagc gctcatcagg
      721 agagcgggcg gcaggctggt gctggcacac cccggagatc ccaatgggac atccctggta
      781 tcgctgacgc ctctggttga gcagcagcag gatatcatcc gggattcgat gctcgaatat
      841 atcgacggag tcgaatgctg gcactcgcgc cacgagaagg ccgtcgtcca gtcgtacgcc
      901 gatttcgcga agcgtcactc cctgatggcg acaggcgggt ccgactgtca ccagcagccc
      961 ccggtgatgg ggactgtcga ggtccccggc tgggtggccg gccagttcga tttctgaaca
     1021 aagcggcaag ttcttctttg cctcccgtca acttgtggta taaaagaaat gcggtgtgtc
     1081 cttcattcta tgcttcgggg ggaccatcgg atcgattata tgtgccatgg aggatgtgat
     1141 tcatgaggga gccgttcaag gattttgtcg agcgtgagat cgtggacgat gcggtattga
     1201 tcgcgtgcgg cctgccggcg accaacaaga ccgaaacaac cgaggtgatc gcccgcatca
     1261 aaggttatgc catactccgg accgatctga tccgtcgcga tgtcctgaag ggtcaggaca
     1321 tcttcgatga aaaggtagcc tctgacatga acaagagaaa cctcgtctac gagaagatgt
     1381 tttcactggc ggacgaactg gcctctcagg gcaggggagt gatcctggac gccaccttca
     1441 tcaagcagtc cctgaggaaa cgggcggcgg cggcggcggc acggaacaag aggaccttca
     1501 tcatccagca gacgagtgct cctgaggcct attctctcaa caagatctca aagcgcacga
     1561 aagagaatta cgaatccaac gcgcttaccc ccgaggccta cttcaacaac aaggagaagt
     1621 tcgagcctgt ggatctcgat gagatcaagc gactctatcc ggatctctcc atcgttcatc
     1681 tggtggtgga cacggcgtcg gacaacgagg acggctggta tgtcatcagc agggaaaccc
     1741 gatagatgat cattcataag gcggacaaca tgaaaaaaat atggatcccg gttcttattc
     1801 ttctcctggc ggcggggtgc gcctccactt tcaaacagaa acgcgaactc tcgaagccca
     1861 tggtcgccat cgccatgggc aagatacagc agggcgagat acagggggcc ctcgtcgagc
     1921 tcaggcgggc agttcaggta aatccaaagg accccgaggt atactatgca tacgccctcg
     1981 cctactggag cacagaggac ctcgacaggg cgctggagaa tatcgataag gccgtgaatc
     2041 tggccggcag gctcggcctg gaacatcccg gcctcaagag cgaggcatac aatctcaaag
     2101 gcaccatcct ggtgggcatg ggggaccatg acgcggcaat caaggccttt cagaacgccg
     2161 tgaaagacga gctctacgcc acacccgaat atgcctatta caacatggca tccgtgtact
     2221 gcagccagaa actctatccc gaggcggaac agtccgcaaa ggaagcgctc cagaaaaatc
     2281 ctcactatgc accggcctgg aagatcctgg ggcagtcgtt catgaatcag gataaggatg
     2341 cccaggccat cgaggccctc cagaaggcga tcctcgaata tccccagtac accgaagcac
     2401 actgggagat cgcccaagtc tacttcaaga caggaaatac ccgggaagcc gtcaagtcct
     2461 tgcgagaggt cgtcatcctg gatgaaaacg ggctgcttgg cgccagggcg cggcagagcc
     2521 tccaggaact gggggcatcg gtgcggtagc ggagagtcat ggacagcaga gccctcgtat
     2581 ggataacccg cgatgaagcc gaggacctcg ggataaaagg ggacgagttt cctttgagga
     2641 tcctggatat caacagcctg atatac
//
LOCUS       DAOQTR010000318         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46193_length_2653_cov_4.402232, whole genome shotgun sequence.
ACCESSION   DAOQTR010000318 DAOQTR010000000
VERSION     DAOQTR010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46193_length_2653_cov_4.402232"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKN85_07920"
     CDS             <1..326
                     /locus_tag="PKN85_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HNT44375.1"
                     /translation="AEREGIDHFVSTIKGRSRSTAYPEGKPITVTYVAEKASGRLIGA
                     QMAGNEGVAHRIDTLATALYNRMTLEDIARLDLAYAPPFATVWDPILVAANVALKRLQ
                     KERQL"
     gene            441..881
                     /locus_tag="PKN85_07925"
     CDS             441..881
                     /locus_tag="PKN85_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT44376.1"
                     /translation="MIEKSQWGEGLSREDMERFAQYLYVARAQPSEPIFNEGAIEPYM
                     CFVADGLVKVTKGDSKKNVKTICEIGPGRTVGEMSIIDGLPRSASAVAVEETTVLVLT
                     KENFEVLLNDNPKLGVALFRRLAQMISHRLRVSDWMLVEYIYES"
     gene            complement(1107..1544)
                     /locus_tag="PKN85_07930"
     CDS             complement(1107..1544)
                     /locus_tag="PKN85_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44377.1"
                     /translation="MNPERIEADFYERTTIYGYRCNSCGYILDESKGGYLYLQNDRGD
                     RIPLKDHGEREIIAQMLRMEEESLHDCEALAASQEAMIDLMEERVGYLSACVCIDCLG
                     QFGLDLRKDALVCPHCRSEHIRTLLEMTDKRCPSCSTGRMERF"
     gene            complement(1525..2004)
                     /locus_tag="PKN85_07935"
     CDS             complement(1525..2004)
                     /locus_tag="PKN85_07935"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNT44378.1"
                     /translation="MHIYFAHPCFNESQETFKREFLGRLRVALGQTDYGKAIGIVDPF
                     DDTPNIEGNRETKLKLSRTVKETCLRMLEDCDIIVALVDDGDTGVAFEAGYAHAINIP
                     IILVSKANCDEANAMLIGAARERIDNILQEEQIGKLARMFEWYYISKERDGHEPGKN"
     gene            complement(2015..>2653)
                     /locus_tag="PKN85_07940"
     CDS             complement(2015..>2653)
                     /locus_tag="PKN85_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44379.1"
                     /translation="MKRDQKNETFRMSGMGGRPAGLTVIELTIVVIIICIIVGLVIKG
                     QSLIVKGQMKQIFKQKQDIAAAFSRYYDHFAYFAGDDPVASSRYSGATNGNGNGRVGV
                     GSSVTAPDFSCTGTGTEQCDLWFELRQANLLGGTGFTNPRHVFSGNVALTYNAPGTTG
                     AGHWLAFEGMPSDVCRGLDKAYDDGVWNTGSIQGASDYDTARDGNFTLFFRL"
BASE COUNT          577 a          776 c          747 g          553 t
ORIGIN      
        1 acgcggaaag agaaggcatc gaccatttcg tgtccaccat caagggcagg tcgaggagca
       61 ccgcgtaccc cgagggcaag ccgatcacgg tgacctacgt ggccgagaag gcatcgggca
      121 ggcttatcgg cgcccagatg gcaggcaacg agggcgtcgc ccacagaata gacacgctcg
      181 cgacggccct gtacaacagg atgacactcg aggacatcgc ccggttggac ctcgcctacg
      241 cgccgccttt cgccacggta tgggacccga tactcgtcgc cgccaacgtg gccctgaaaa
      301 gattgcaaaa ggaaaggcaa ctatagtata cagttgatac aaccaggtga gagcataact
      361 gttaacagat ccaagaatgc tgaggtccag gagaggccat gctgcaccac gcgttgtcat
      421 ccgttgaaga acgtatttcc atgatcgaga agtcccaatg gggggaagga ttgagccgtg
      481 aggacatgga gcggttcgcg cagtacctct acgtcgccag ggcgcagccg agtgaaccca
      541 tcttcaatga aggggccatc gaaccctaca tgtgtttcgt cgccgacggg ctcgtcaagg
      601 tcaccaaggg ggactccaag aagaacgtca agaccatatg cgagatcggc cccggtagaa
      661 cggtcggcga aatgagcatc atcgatggcc tgccccggtc cgcgtcagcg gtggcagtgg
      721 aagaaacgac ggtcctcgta ctcaccaagg agaacttcga ggtcctcctg aacgacaacc
      781 ccaagctcgg agtcgccctc ttccgaagac tcgcgcagat gataagccac agattgcggg
      841 tgtcggactg gatgctcgtc gaatacatat acgaatcgta gacgccgtcc caagtccgcg
      901 acaacagggg agttctggaa gtcccggtcg ttcctgacac ctgaagactc gtcgtcccgg
      961 ctccggaatt gggccctcgc ggtccgccac cccggctttc agccggaatg gcggaggagg
     1021 aagtgggggg atgactgagg agtacgttgc cggaatggcg ggaaaccttc aggtcgtccg
     1081 gcaacatcta aatccatata cgaaccctaa aagcgttcca tccgtcccgt gctgcagctc
     1141 gggcatcgtt tgtcggtcat ttccagcagg gtcctgatgt gctcggacct gcagtgggga
     1201 caaacgaggg catcttttct gaggtcgagg ccgaactggc cgagacagtc gatgcacaca
     1261 caggcggaga gataacccac gcgctcttcc atgagatcga tcatcgcctc ctgagaggcg
     1321 gcaagcgcct cgcagtcatg aagggattcc tcttccatgc ggagcatctg ggcaattatc
     1381 tcgcgctcgc cgtggtcctt cagcggtatc ctgtcgcccc tgtcgttctg cagatagaga
     1441 tacccaccct tcgactcgtc gaggatgtac ccgcaggaat tgcagcggta tccgtagatc
     1501 gtggtccttt cgtagaaatc tgcctcaatt ctttccgggt tcatgaccat cacgctcctt
     1561 tgatatgtag taccattcaa acatccgggc aagctttcca atctgctcct cctggagtat
     1621 gttgtctatc cgttccctcg ccgcgccgat gagcatggcg ttggcttcgt cgcagtttgc
     1681 cttcgatacg aggatgatgg ggatgttgat cgcgtgggca taaccggcct cgaaggccac
     1741 ccccgtgtcg ccgtcgtcga cgagggcaac aatgatgtcg cagtcctcca gcatccgaag
     1801 acaggtctcc ttgacggtcc ggctcagctt gagcttcgtt tccctgttgc cctcgatatt
     1861 gggcgtgtcg tcgaagggat cgacgatgcc gatcgctttt ccataatcgg tctgtcccag
     1921 tgcgaccctc aacctcccca ggaattccct cttgaaggtc tcctgggatt cgttgaaaca
     1981 gggatgagcg aagtagatat gcatggtcac ctcatcacaa cctgaaaaag agtgtgaaat
     2041 ttccgtctct ggcggtatcg tagtccgaag cgccctgtat gctgcccgtg ttccagacac
     2101 cgtcatcgta ggccttgtcc agacccctgc acacatcgga gggcatgccc tcaaatgcca
     2161 gccagtgacc tgcccccgtc gttccgggag cgttgtacgt gagggccaca tttccgctga
     2221 atacatgcct ggggttcgtg aatcctgtcc cgccgaggag attcgcctgc ctcagttcga
     2281 accagaggtc acactgctcc gttcccgtcc ccgtgcagga aaaatcaggg gccgtcaccg
     2341 acgaaccaac gcctacccgc ccgttcccgt tgccgttcgt ggcaccgctg taccgggagg
     2401 aagccaccgg atcatcgccg gcaaaatatg cgaaatggtc ataataccgg gagaaggcgg
     2461 cggcgatatc ctgcttctgc ttgaagatct gcttcatctg gcccttcacg ataagggact
     2521 gtccttttat aacgagacct acgataatgc agatgatgat cacgacaatg gttagttcga
     2581 tgacggtgag gccggcaggt ctccctccca taccggacat cctgaaagtt tcgttctttt
     2641 ggtctcgttt cat
//
LOCUS       DAOQTR010000319         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46343_length_2648_cov_3.539915, whole genome shotgun sequence.
ACCESSION   DAOQTR010000319 DAOQTR010000000
VERSION     DAOQTR010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46343_length_2648_cov_3.539915"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1216
                     /locus_tag="PKN85_07945"
     CDS             230..1216
                     /locus_tag="PKN85_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44380.1"
                     /translation="MYVAGWQRVKVFSAVFALVLLFVPFRPASGQEIFVNGGIAGGAH
                     KGTSTRGHWSVAYFQHLTEHATVSASYINEGHQPDHDRDGIAFQIWGRGAPLGPGVSF
                     AGGVGPYLFSDTREGTSGYGCEIQHGLGAIFSGAITWYALSPLLLQARGNYINGSNGF
                     DTLSVSFGIGYLLGDEGRGNPPPGGHSTKNEVGLFMGGTALNCDHSSALSVGLEYRRK
                     LSPNFDWTVAGLHEGDKRPVGRYGVMSEVWAVRAFYGDRLGLGIGAGAYLARDRYGGD
                     EGGARDDVNAVVSLTAVWWFTPRLGLRAVFHRVATDHDRDADVLVGGLAISF"
     gene            complement(1228..1794)
                     /locus_tag="PKN85_07950"
     CDS             complement(1228..1794)
                     /locus_tag="PKN85_07950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNT44381.1"
                     /translation="MADEQGGKVKVLGFAGSLRKGSFNKMLLNAAVKVAPADVELEVF
                     DIGGIPPFNQDLENEPHEKVRELKEKVRAADGVLIVTPEYNYSVPGVLKNAIDAASRP
                     YGTNPFEGKPVAIMGASIGTLGTARAQYHLRQTCVFLDCFVLNRPEIMVPFAEQKFDA
                     SGNLMDDRTKQMIEELLAELAKWARKLR"
     gene            1972..2472
                     /gene="def"
                     /locus_tag="PKN85_07955"
     CDS             1972..2472
                     /gene="def"
                     /locus_tag="PKN85_07955"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT44382.1"
                     /translation="MSTIDIRVIPDPVLRKLAKPVENITDAVLKLSGDMVETMILAHG
                     AGLAAPQIGLPIRLITIDASLNPTEEKPIILINPVIVETHLEENGEEGCLSVPGYYEY
                     VRRAKKVHARGTDLRGNIVDFECEGQLARAFQHEIDHLNGVLFVDLLSPIKKNIFKKK
                     YTGKKA"
     gene            2469..>2648
                     /locus_tag="PKN85_07960"
     CDS             2469..>2648
                     /locus_tag="PKN85_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44383.1"
                     /translation="MRIVFFGSSAFSVPSLLKIKDSVVAVVTKKARPRGRGYQIDDNE
                     VKSAALSLNVPLVEIG"
BASE COUNT          529 a          747 c          797 g          575 t
ORIGIN      
        1 cgtctgcgac ggcaggtagg aaagaggctt tctttcagtg agaccggaga aagggattgt
       61 tgctgtagca ccattggatg acagggtcga gtgcaaaagt actacgtgat taatgtcggt
      121 gctgtgacag agagaggaga ctgggtgtgt tagtctgtac ttgatgcatg tccgatcggg
      181 tgttgtgcgg tccgcaccca cggtcccgtc gtgaagtgag gaggtcttcg tgtatgtcgc
      241 aggctggcag cgtgtgaagg tcttttccgc cgtgttcgct ctggtgctcc tcttcgttcc
      301 tttccgtccc gcttcaggcc aggagatatt cgtaaacggg gggattgccg gaggcgcgca
      361 caagggcacg tccaccaggg gccattggtc tgtggcatat tttcaacacc tcaccgagca
      421 tgcaacggtc agcgcgtcct atatcaacga gggacaccaa ccggaccacg acagggacgg
      481 tatagcgttc cagatatggg gccggggtgc cccgttgggt ccgggtgtct ccttcgccgg
      541 aggggtcggt ccctatttgt tctccgatac gcgagaagga acatcggggt atggctgtga
      601 gatccaacat ggtctggggg ccatcttcag cggcgcgatc acgtggtacg ccctgagtcc
      661 ccttctgttg caggcgcgcg gcaactacat caacggctcg aacggcttcg acaccctttc
      721 ggtctccttc ggtatagggt atctgctcgg tgacgaaggc cgagggaacc ctccacccgg
      781 cggtcattcg acgaagaacg aggtgggcct cttcatgggc gggaccgctc tgaactgcga
      841 tcacagcagc gccctctctg ttggtcttga ataccgtcgg aagctctccc ccaatttcga
      901 ctggacggtg gcggggcttc atgaaggcga caaacgccct gtcggccggt acggagtgat
      961 gtccgaggtg tgggcagtgc gggcgttcta tggcgatcgc ctcgggttgg gcatcggcgc
     1021 gggagcgtat cttgcccggg acaggtacgg gggagatgag ggaggcgcga gggacgatgt
     1081 gaacgcggtg gtttccctga cggctgtctg gtggttcaca ccccgcctgg ggctccgcgc
     1141 ggtctttcat cgggtcgcca cagaccacga ccgcgacgcg gatgttctcg tgggtggcct
     1201 ggcgatctcg ttctgaagcc gccctgttca gcgaagcttc ctggcccact ttgccagttc
     1261 ggccaggagt tcctctatca tctgctttgt cctgtcatcc atgaggttgc ccgaggcatc
     1321 gaacttctgc tccgcgaaag ggaccattat ctcgggccgg ttgagaacga aacaatcgag
     1381 aaagacgcat gtctgccgca gatggtactg cgccctggcg gtcccgagcg tgccgatgga
     1441 agcacccatg atggcgacgg gttttccctc gaaggggttc gtaccgtacg gcctcgacgc
     1501 ggcgtcgatg gcattcttca ggacccccgg aacggagtag ttatactcag gcgtgactat
     1561 caggacgcca tcggccgccc tgaccttttc cttcaactcc ctgaccttct cgtgaggctc
     1621 gttctcgaga tcctgattga aaggggggat ccccccgatg tcgaatacct ccaactccac
     1681 atccgccggc gcgaccttga cggcggcatt gagaagcatc ttgttaaagg accctttcct
     1741 caaactcccc gcgaaaccca gcactttcac ttttccacct tgttcatcgg ccatgggaac
     1801 ctccttgcac ggcatgtggg atgaaatgaa gctaagtcct cccggcaatg gtgtcaacgg
     1861 taaagacctc gcctctcgac agctcccacc ccgcgatcaa ggcacccgat agaatgtgag
     1921 atagggcgtt ccagttattg actctgcgcg cggatggttt taaaatagtg catgtccaca
     1981 attgatattc gtgtcatacc agatcctgtt ctcaggaagc ttgcgaagcc tgtcgaaaac
     2041 atcacggacg ctgtcctcaa gctctccggg gatatggtcg agacgatgat cctggcccat
     2101 ggagcaggtc tggcggcacc gcagatcggg ctcccgatca ggctcataac cattgacgcc
     2161 tctttgaacc ccacggagga gaagcccatc attctcatca atcccgttat cgtcgagacc
     2221 cacctcgagg agaacggcga ggaggggtgc ctgagcgttc ccgggtatta cgagtacgtc
     2281 aggagagcga agaaagtgca tgcccgtggt acggacctca gggggaacat cgtcgacttc
     2341 gagtgcgagg gacagcttgc gcgcgcgttc cagcacgaga tcgaccatct caacggcgtc
     2401 ctcttcgttg atcttttaag tcccataaaa aagaacattt tcaagaaaaa gtatacaggc
     2461 aagaaggcat gaggatcgtc tttttcggat cctccgcctt ctccgtaccg tcccttctaa
     2521 agatcaaaga ttcagtggtt gccgttgtga ccaagaaggc gcgccccagg ggcaggggat
     2581 accagatcga cgacaacgag gtcaagagcg cggccctgag cctcaatgtg cccctcgtcg
     2641 agataggc
//
LOCUS       DAOQTR010000320         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46417_length_2645_cov_3.577992, whole genome shotgun sequence.
ACCESSION   DAOQTR010000320 DAOQTR010000000
VERSION     DAOQTR010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46417_length_2645_cov_3.577992"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PKN85_07965"
     CDS             <1..988
                     /locus_tag="PKN85_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44384.1"
                     /translation="LRTGLVVALEDGILLFIAIVLPWYLAVYAREGFPYLYENILRQN
                     FLRFFDAWSHKRPFYYYFTTLPLDFFPFSLFLPLGLYAAVRNWKTDRSTRFFTLWFVW
                     FFFFLSLSSGKISKYMLPALPALAVIVSGTITDTKSIYNRIAFGVTAGVFAALGIGLI
                     VYRTSLYGEFLPERVILGSLAICAGFLVFLSMRHHRARAAFVSIAAFMALAYMTGNIS
                     IFEKWNRYKSPKPMAQKIRALAGDTVPWVYYGSMRGVYVYYVGRLAVHVEEHDVAGLK
                     DLASRRREFYILTRGRDADEVTGALPGAVVETEDRIGDTAMVLFKFTREDGG"
     gene            994..2637
                     /locus_tag="PKN85_07970"
     CDS             994..2637
                     /locus_tag="PKN85_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT44385.1"
                     /translation="MRPGKTFIKYTIILLVLSYAFIFYGLGDYSLKEPDEGRYAEIPR
                     EMVESGDYTVPRLNDVRYFEKPPFLYWAVAVSYKLFGVSEWSFRFPNALAAFLCVMCL
                     FFFGRRWTDEEAAFLASLVLLSSFGFFGMARIVTTDMVLTFWLFLALLCFYEHYRKRG
                     GAFVYGFYGAMAMATLTKGPVAPVLLCGTVLIFLLAQRDISFVRHMKPLTGFFLYFAM
                     AAPWFVIISVKEKEFVDFFFVDQHFLRFTTRKHDRGGPLHYFIPVLLGGMLPWSLFIP
                     RAVASTWRRPDCRFFILWSALVFVFFSVSGSKLPPYILPAFPALSLPLGILFHESRGS
                     RFRRHWEIVVYGLLMAIFAFSCLLYFSDDFMAYIADISMDATSITLDLKHFSLWMSLT
                     SAACLVLFLLRSFRDPGRLFVILLLFSLSTIIAIMAHSGVVDRINTAKKLALAASELE
                     TRPDIVVNYSGLDLTIPFYLKRPVVIASYKGELAMGAKYDDARKIFMEEAEFFRLLGS
                     DRKVLFITKSKRVKNLEKRFPGRITTHLCQNDRCLLSNY"
BASE COUNT          474 a          845 c          690 g          636 t
ORIGIN      
        1 gctccggacc ggcctcgtcg tcgccctcga ggacggcatc ctcctcttca tcgccatcgt
       61 ccttccctgg taccttgccg tgtatgcccg cgagggtttt ccctacctct acgagaacat
      121 tctccgccag aacttcctgc ggttcttcga cgcctggagc cacaagaggc ccttttacta
      181 ctattttaca acgctccccc tggatttctt ccccttcagc ctcttcctgc cgcttggcct
      241 ctacgcggcc gtcaggaact ggaagacaga ccggtcgaca cgcttcttca cgttgtggtt
      301 cgtctggttc ttcttcttcc tttccctctc atcgggaaag atcagcaagt acatgctccc
      361 ggcgctgcct gccctcgcgg tcatcgtttc gggaacgatc accgacacga agagcatcta
      421 caacaggata gccttcggtg tgacggcggg agtctttgcc gccctgggaa taggcctgat
      481 cgtatacaga acgtccctgt acggagaatt cctccccgaa agggtcatcc tcggttcgct
      541 tgccatatgc gccggctttc tcgtctttct ctcgatgcga catcaccgcg cgcgggcggc
      601 ttttgtctcg atagccgcct tcatggcact cgcgtacatg acggggaaca tctccatatt
      661 cgaaaagtgg aaccggtaca aatcaccgaa acccatggct cagaagatac gggcactcgc
      721 gggcgacacc gtgccctggg tctattatgg cagtatgcgc ggagtttatg tatactacgt
      781 cgggcggctg gccgtccacg tggaggaaca tgacgtcgcc gggcttaagg acctcgcgtc
      841 gcgccggagg gaattctaca tcctgacccg cggacgcgac gcggacgaag tgacgggtgc
      901 cctgcccggg gccgtcgttg agacggaaga ccgcatcggg gatacggcga tggtcctttt
      961 caagttcacg agggaagacg gcggatagag atcatgagac cgggaaagac cttcatcaaa
     1021 tacaccatca tccttctcgt cctgtcgtac gccttcatct tctacggtct cggtgactac
     1081 tccctgaagg agcccgatga agggagatac gccgagatac cccgggagat ggtggaaagc
     1141 ggggactaca ccgtcccgcg actcaacgac gtgcggtatt tcgagaaacc gcccttcctc
     1201 tactgggccg tggcagtatc gtacaagctc ttcggggtga gcgagtggtc attcaggttt
     1261 cccaacgccc ttgccgcttt cctctgcgtc atgtgcctct tcttcttcgg ccggaggtgg
     1321 accgacgagg aggcggcctt ccttgcctcc ctggtcctcc tgtcgtcctt cggatttttc
     1381 ggaatggcgc ggatagtcac cacggacatg gtcctcacct tctggctctt tctggccctc
     1441 ctgtgcttct acgaacacta ccggaagaga gggggcgcct tcgtctacgg attctacgga
     1501 gccatggcga tggcgacgct cactaaaggc cccgtcgcgc cggtcctctt gtgcggtacc
     1561 gttctcatct tcctcctcgc gcaaagggat atctccttcg taaggcacat gaagcctctc
     1621 acgggcttct tcctgtattt cgccatggcg gcgccgtggt tcgtcatcat atcggtgaaa
     1681 gaaaaggagt tcgtcgactt cttcttcgtg gaccagcatt tccttcgctt caccaccagg
     1741 aaacatgaca gaggagggcc cctgcattac ttcatacccg ttctcctggg ggggatgctg
     1801 ccctggtcgc ttttcatccc ccgcgccgtc gcctcgacgt ggagacgccc ggactgccgt
     1861 ttcttcatcc tgtggtccgc ccttgtgttc gtcttcttca gcgtctccgg ctccaagctt
     1921 ccgccgtaca tcctccccgc cttccccgcc ctttctcttc ctctgggcat ccttttccac
     1981 gagagccgcg ggtcccgttt tcggcggcac tgggagatcg tcgtctacgg actcctcatg
     2041 gccatatttg ccttctcctg cctgctctat ttcagcgacg atttcatggc atatatcgca
     2101 gacatttcga tggacgcgac atccatcacg ctcgacctga aacacttttc cctctggatg
     2161 tctctcacct cggctgcctg tctcgtcctt ttcctcctgc ggagcttcag agacccggga
     2221 aggcttttcg tcattctgct tctcttctct ctttccacca tcattgccat catggcccat
     2281 tccggggtgg tcgacaggat caacacggcc aagaagctgg cgctggccgc gtcggaactg
     2341 gagacgcgac cggacattgt cgtgaactac tccggactcg acctgacgat acccttctat
     2401 ctcaaacgtc ctgtcgtcat cgcgtcgtac aagggagagc tcgccatggg cgccaagtat
     2461 gacgacgccc ggaagatatt catggaggaa gcggagttct tccgccttct cggctccgac
     2521 aggaaggtcc tcttcatcac gaaatccaag agggtaaaga acctggagaa aaggttcccc
     2581 ggacgtataa cgacccatct gtgccagaac gacagatgtc tcctgtcgaa ctactgaccc
     2641 ctgag
//
LOCUS       DAOQTR010000321         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46554_length_2640_cov_3.868085, whole genome shotgun sequence.
ACCESSION   DAOQTR010000321 DAOQTR010000000
VERSION     DAOQTR010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46554_length_2640_cov_3.868085"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="PKN85_07975"
     CDS             <1..1750
                     /locus_tag="PKN85_07975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNT44386.1"
                     /translation="AVAYPIPRRTSTDVPALDILGVILGDGESSRLQEALKNKKGVVN
                     GIETYVFTPKEEGLFVIYANHSANDRDSVLREIDREIGRIQKQGIKDWELVKARNMLR
                     AYYVYDAETAQGRARQIGDAKTMTGDPHFIEKFLKDVDRVSADDVRKAAVKYLRPDTR
                     RIVVMGPAKAVKNPYRKTLGNGLTCLVNRNTSSPTFSFRIGFTGGVKAEQPGRNGEFN
                     LLARMLLKGTKNKKSSEIARQIDLLAGTLVPYNGRNLFGLSGRFLSKDVKEVLRLLKE
                     LLMETSMTERELTRVKREVLSEIRQRDDDPISFTFLRFNEAIFAGHPYSRDPMGSEKD
                     VEALSLTDLEGVYRKYVTPHGAVLALSGDIDEKEVFSWFEELFGRWEGPESPLARVEA
                     GRAGKEVAVEKDIQQTHMVFGFVGPGLTDKDRYAAEVLDAVLSGMGGRIHRVLREENP
                     YAYAVTFFNQMAYETGAMGIYIGTSEKFAKDVIRIAREEIEKIRREGISEKEVQDGKN
                     YLIGNHYIRMQSNGAIVPSMCFDALYGLDPDLFKTWPGFIEKVTKEDVDRVARRYLTL
                     DSMVRLVVGKIKDNNK"
     gene            complement(1824..>2640)
                     /locus_tag="PKN85_07980"
     CDS             complement(1824..>2640)
                     /locus_tag="PKN85_07980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT44387.1"
                     /translation="DSDMNIDPAKVEKAITPKTKAIIVVHLFGKICDMKRILGIAEKR
                     GISVVEDMAQALGAKRGGTAVGTFGDIAATSFYPTKNLGGIGEGGMVLTRRADLGEMA
                     RKLRVHGMGEKPYIHEVIGFNSRLDEIKALALCVKLRKLASWNKTRLETAGYYNMHMR
                     DLPLVLPTIDDETSHIFHHYVIRTVRRDALQLHLREKGIMTGIYYPLPLHLQPCFSYL
                     GYKEGDLPIAEEAARTSLAIPVFPELKKREKDYIIKGIRSFFEKTDEGSENGQ"
BASE COUNT          597 a          739 c          779 g          525 t
ORIGIN      
        1 tgccgtcgcc tatccgatcc ccaggaggac aagcaccgac gtcccggccc tcgacattct
       61 cggcgtcatc ctgggagacg gggagagttc caggctccag gaggccttga agaataagaa
      121 gggtgtcgtc aacggaatag agacatatgt cttcaccccc aaggaagagg ggcttttcgt
      181 catctacgcc aaccattcgg ccaatgacag ggacagcgtg ctccgggaga tagacaggga
      241 gatcgggagg atacagaagc agggcatcaa ggactgggaa ctcgtcaagg caaggaacat
      301 gctgagggcg tactacgtct atgatgccga gacagctcag ggcagggccc gtcaaatagg
      361 cgacgccaag accatgacgg gagatcccca ttttatagag aagttcctca aggatgtcga
      421 cagggtatcg gcggatgacg tccgaaaggc ggcggtgaaa tatcttcggc ccgataccag
      481 gcggatcgtt gtcatggggc ccgccaaggc ggtgaagaac ccgtaccgaa agacattggg
      541 aaatggcctg acatgcctcg tgaacaggaa cacctcgtca ccgaccttct ccttcaggat
      601 cggtttcacc ggcggtgtca aggcggagca accgggaagg aacggtgagt tcaatctcct
      661 tgcacggatg ctcctgaagg gaaccaagaa caagaaatcc tcggagatag ccaggcagat
      721 agacctcctc gcgggcacac tggtgcccta caacgggcga aatctcttcg gcctgtcggg
      781 aaggttcctg agcaaggacg tgaaagaggt cctccgactc ctgaaggaac tcctgatgga
      841 gacgtccatg accgagcggg aactcacccg ggtgaagaga gaggtcctct ctgagatacg
      901 ccagcgggat gacgatccga tctccttcac cttcctgcgc ttcaacgagg cgatattcgc
      961 cggccatcct tacagccgcg accccatggg gagcgaaaag gacgtcgaag cgctgtccct
     1021 cacggacctt gagggcgtgt accgcaaata cgtcacgccc cacggtgccg tccttgccct
     1081 atcgggagac atcgacgaaa aagaggtctt ttcatggttc gaagagttgt tcggccgctg
     1141 ggagggacca gaaagtcccc ttgccagggt ggaagccgga cgggccggca aggaggtcgc
     1201 cgtcgagaag gacatccagc agacccacat ggtcttcggc tttgtcggtc cagggctcac
     1261 ggacaaggac cggtacgcgg cagaggtcct cgacgcggtg ctcagcggta tgggcggcag
     1321 gatacaccgg gtcctcaggg aggagaaccc ttacgcctat gccgtcacct tcttcaacca
     1381 gatggcctac gagacggggg ccatggggat ctacatagga acgagcgaga agttcgcaaa
     1441 ggatgtgatc cgtatcgccc gcgaggagat cgaaaagatc cgccgggagg gtatttccga
     1501 aaaagaggtc caggacggga agaactacct catcggcaac cattacatca ggatgcagtc
     1561 gaacggcgcc atcgttccgt ccatgtgctt cgatgcgctt tacggccttg acccggacct
     1621 tttcaagaca tggccggggt tcatcgaaaa agtgacgaag gaggatgtcg accgggtggc
     1681 ccgcagatac ctcacccttg actccatggt cagactggtg gtggggaaga taaaggacaa
     1741 taacaaatga ccggggatgg ccgggccttt cccatgaccc atcacctata acccatatcc
     1801 tgcgttatta ccccttcttc tggtcattga ccattctcac taccttcatc cgttttctca
     1861 aaaaaggacc ttattccttt gattatatag tccttttccc tcttcttcaa ttccgggaaa
     1921 acggggatgg cgaggcttgt tctggccgcc tcttcggcga tgggaagatc gccttccttg
     1981 tatcccagat acgaaaaaca gggctgcaga tgaaggggga gaggatagta tatgccggtc
     2041 atgatcccct tttccctgag gtggagttgg agggcgtcgc gccgcaccgt tcggatcaca
     2101 taatgatgga agatgtgtga tgtctcgtcg tcgatggtgg gaaggacgag gggaaggtcc
     2161 ctcatgtgca tattgtagta accggcggtc tcgagacggg tcttgttcca ggaggccagt
     2221 ttccttaatt tgacgcagag cgccagggcc ttgatctcgt cgaggcggct gttgaacccg
     2281 atcacctcat ggatataggg cttctcgccc attccgtgga cacggagttt gcgcgccatc
     2341 tcaccgaggt cggcacggcg ggtgagcacc atgcccccct cgccgatacc cccgaggttc
     2401 ttcgtgggat agaaactggt cgccgcaata tcgccaaagg tgcccaccgc cgtcccgccg
     2461 cgcttcgccc ccaaggcctg cgccatatcc tccaccaccg agataccacg tttttccgcg
     2521 atgccgagga tccttttcat gtcgcagatc ttcccgaaga gatgcacgac gatgatcgcc
     2581 ttcgttttcg gggtgatcgc cttctcgacc ttcgccgggt cgatattcat gtcgctgtcc
//
LOCUS       DAOQTR010000322         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46566_length_2640_cov_3.053385, whole genome shotgun sequence.
ACCESSION   DAOQTR010000322 DAOQTR010000000
VERSION     DAOQTR010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46566_length_2640_cov_3.053385"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..741
                     /locus_tag="PKN85_07985"
     CDS             259..741
                     /locus_tag="PKN85_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT44388.1"
                     /translation="MKIAVISDTHLAHPTEGFKRAMKALFSDVDMMLHAGDMTSSSVF
                     DYLSNWDLRAVRGNMDDFDLGARLPESRVEEAAGRRIGIIHGWGSPQGLEDKVLRVFR
                     DVDIIVFGHSHIPFNMKKGGAILFNPGSCRGGYSVSGTVGIIEIGAEVTLRHLKVDEL
                     "
     gene            772..>2640
                     /locus_tag="PKN85_07990"
     CDS             772..>2640
                     /locus_tag="PKN85_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNT44389.1"
                     /translation="MDGICISFLWHMHQPYYKNTFTGEYLLPWTLLHGTKDYYDMGAM
                     LKGFPGMKQNFNFVPSLLEQLVDYEDLDVKDTYLDVFRKDPSHLTEKNKVFLLGNFFN
                     ANWENMIRPFPRYYELLAKRGLYCPADGLSKVTGYFSEDEFRDLQILFFLAWTDPSFF
                     ADFEGLRYLREKGKKFSEEDKKVLMDVQKNILKGTIPLYRELAAEGSVELSTSAFYHP
                     IIPLLIDSASAREAMPDVNLPDVPFAWPEDASRQISSGLDFFQRVMGIRPAGMWPPEG
                     SVSEAALELYMRHGVRWLATDEDILFESLRWGARRDGHGFVINPEVLYKPYRYERNGR
                     DVTIIFRDQSLSDLISFHYSRSDAREAAEDCLGRLRTIRGSIGNRIARPLVTIAMDGE
                     NAWEGYKNDGRDFLSYLYEGILREDGMTPVTISEYLETARDYGSLSRCHAGSWIGHNF
                     SIWLGHSEDNSAWSRLAETRRFLGEEDPDHANSMAWESIYVAEGSDWFWWYGDEHSSD
                     SDEIFDLLFRENLANVYRYLGKEVPEILSIPIIVEDRVARPAREPVNFLRPVIDGRVT
                     NYFEWIGSGFIEGKGHGTAMHEAVFALKDCCFGFNETSLFLRVDLDKRFMDEMDGAV"
BASE COUNT          570 a          729 c          767 g          574 t
ORIGIN      
        1 agtgaggatg ccgagggaga gaagcaggat gccggtgagt tctttcagga gatatatgtt
       61 gaacttgagg ggaattttct tcaataattt catcacttac tctaccaccc cacgcggtgt
      121 cctgtcaatg cagatgggca tcggccttgg gtgcggatgc ggaaggtgcc agaagaaggg
      181 aaagcctccc gcctgccctg gtaacgggca aatttgtttt tgacaggccc ccctttttct
      241 tatacagtta ttcatccaat gaagatcgcc gtcatttcag acactcatct cgctcatccg
      301 acagagggtt tcaagcgcgc tatgaaggcc ctgttcagcg atgtcgacat gatgctccac
      361 gccggcgaca tgacgtcgtc gagcgtcttc gactaccttt cgaactggga cctccgggcg
      421 gtcaggggca acatggacga tttcgacctg ggggcccgtc tcccggagag ccgtgtagag
      481 gaggccgcgg gaagacggat agggatcatc catgggtggg gttcgccgca agggcttgag
      541 gacaaggtcc tccgggtgtt ccgggatgtc gatatcattg tcttcggcca ttcgcacatc
      601 cccttcaata tgaaaaaagg gggggccatc ctcttcaatc ccggaagctg caggggcgga
      661 tactcggtat ccgggacagt cgggatcatt gagatcggcg ccgaggtcac gttgcgccat
      721 cttaaggttg acgaacttta accattcgga attgtgagcg ataatacgaa tatggacggg
      781 atctgcatct catttctctg gcacatgcac cagccgtatt acaagaacac cttcaccggg
      841 gaatacctcc ttccctggac ccttttgcac ggcaccaagg attactacga catgggcgcc
      901 atgctcaagg gttttcccgg tatgaaacag aacttcaatt tcgttccctc cctcctcgaa
      961 cagctcgtgg actatgaaga cctcgacgtg aaagatacgt atctcgatgt gttcaggaaa
     1021 gacccttcgc atctcaccga gaaaaacaag gtcttcctcc tcggcaactt cttcaatgcc
     1081 aactgggaga acatgatccg gcccttcccg cgctactacg aactcctcgc caaacgcggt
     1141 ctctactgtc ccgcggacgg cctgtcgaag gttaccgggt atttcagcga ggatgagttc
     1201 cgggacctgc agatactttt ctttctcgcc tggaccgacc cgagcttctt cgcggacttt
     1261 gaaggtctcc ggtatctcag ggaaaagggg aaaaagttca gtgaagagga caagaaggtc
     1321 ctgatggacg ttcagaagaa catcctcaag ggtacgatcc ccctctacag ggagcttgcc
     1381 gctgaggggt ctgtggaact gtcgacctcg gccttctatc accccatcat tccccttctc
     1441 atcgacagcg cgtcggcgag ggaagcaatg ccggacgtga acctgccgga cgtgcccttt
     1501 gcctggcccg aggacgcctc gcgccagata tcgtcgggtc tcgacttctt ccaaagggtt
     1561 atgggtatcc ggccggcggg catgtggcct cccgagggct ccgtcagcga ggccgccctg
     1621 gagctttaca tgcggcacgg tgtccggtgg cttgccacgg acgaggacat ccttttcgaa
     1681 agtctgagat ggggggcgcg cagggacggg cacggcttcg tcatcaatcc cgaggtcctc
     1741 tacaagcctt atcgttacga gaggaacggc cgcgacgtca ccattatctt ccgggaccag
     1801 tccctctccg acctcatatc ctttcactat tcgcggtcgg acgcaaggga agcagcggag
     1861 gattgcctcg gcaggctcag gacgatccgg ggctccatag ggaacaggat cgcgaggccc
     1921 cttgtaacga tcgccatgga tggcgagaac gcctgggaag gttacaagaa tgacggaagg
     1981 gatttcctga gctatctcta cgagggcatc cttcgcgagg acggcatgac gcccgtcaca
     2041 atatccgaat accttgaaac cgcccgggac tatggaagcc tttcccggtg ccacgccggt
     2101 tcctggatcg gacacaattt ctccatatgg ctcggccata gcgaagacaa ttcggcatgg
     2161 tcccggcttg cggagacccg gcggttcctc ggggaagaag accctgacca cgcgaacagc
     2221 atggcctggg aatccatcta tgtagcggag gggagcgact ggttctggtg gtacggagac
     2281 gaacattcct cggacagcga cgagatattc gatctcctgt tccgtgagaa cctggcgaat
     2341 gtgtaccgct atctcggcaa ggaggtgccg gagatactgt cgatcccgat catcgtggaa
     2401 gaccgtgtgg cgcggcccgc tcgggagccc gtcaattttc tccgtcccgt tatcgatggc
     2461 agggtgacaa actatttcga atggatagga tccggcttca tcgaaggcaa ggggcacgga
     2521 acggccatgc atgaagcggt cttcgccttg aaggactgtt gtttcgggtt caacgaaacc
     2581 agtctgttcc tgcgtgtcga tctggacaag aggttcatgg atgaaatgga tggggccgtc
//
LOCUS       DAOQTR010000323         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46708_length_2635_cov_3.146899, whole genome shotgun sequence.
ACCESSION   DAOQTR010000323 DAOQTR010000000
VERSION     DAOQTR010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46708_length_2635_cov_3.146899"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKN85_07995"
     CDS             complement(<1..730)
                     /locus_tag="PKN85_07995"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HNT44390.1"
                     /translation="MISSPTSKGIIGFTTTIPVELIFAAGYEPCDLNNIFVTDPDPGR
                     FIERAERDGFPKSMCNWIKGIYGVIMEQDMAGVITVMEGDCSNTQALAEILRYRGVRT
                     IPFSFPYDRDREVLAREIEKLGNELSVDEGSLADVELRIAGVREKLSEIDTMTWSERN
                     VTGAENHRWLVGSSDMLGDMEGYGSSAGEFILSARKRESLQGIPLGYIGVPPIVTDLY
                     DFIESVGGQVVYNETQRQFALPYET"
     gene            complement(875..1999)
                     /locus_tag="PKN85_08000"
     CDS             complement(875..1999)
                     /locus_tag="PKN85_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44391.1"
                     /translation="MPVSSRLLQPVSMIVLTGGPCSGKSSSLAYLTEKLSDHGFMVFV
                     VPETATLITGNGIDRRKMDKPGQIVVFEEAIFDMQMSFEDTYKQAVSRIFPERRKVIL
                     LDRGIMDIRAFLTDDVFNSILKKKGLTRAAIRHRYDGVIHLVTAADGAAEYYTGENNT
                     ARLETPEEALRIDLRIKESWLGHPRFKIVDNSTDFEGKIKRAFSAIARFLGIPDVLPT
                     GEKYLVRHVDLAALPAHQRIDMEQVYLRSRDRREVTRIRRRGQDGVYLHFLARTRHAG
                     ARSLPIEEEELIPEQEYLSLARLMDPKTEVLARERICFLWNNQYYELDRYGGRHEGLT
                     MLLAESPTSIPPFVTAGKRVTDDLRYADRTLARRKANKPA"
     gene            complement(2154..>2635)
                     /locus_tag="PKN85_08005"
     CDS             complement(2154..>2635)
                     /locus_tag="PKN85_08005"
                     /inference="COORDINATES: protein motif:HMM:NF018651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44392.1"
                     /translation="SGIRHISMGALLGLTDWRRDVIALFEHVRGLEKRYPGVEFGLAF
                     PRLRRVADDIHQYQEVSDRDMLKIMTAARLFFPRVGISISTRETPRFRDSILEFGVTK
                     MSAGSSTRVGGYLEGEHKYEDGQFEVFDPRSFDEIKAMLRSRGFDPVVTDWRNIVNS"
BASE COUNT          510 a          793 c          736 g          596 t
ORIGIN      
        1 tcgtctcgta aggcagggcg aactgacgct gcgtctcgtt atagaccacc tgccccccca
       61 cgctttcgat gaagtcataa aggtcggtga cgatcggggg aacaccgatg tacccgaggg
      121 gtatcccctg caggctctcc ctctttcgag cgctcaggat gaactcgcct gccgacgagc
      181 cgtacccctc catgtcgccg agcatgtccg aggaaccgac gagccagcga tggttctccg
      241 ccccggtcac gttgcgctcg ctccacgtca tcgtgtcgat ctcggaaagc ttctccctta
      301 ccccggcgat ccgcagttcc acgtccgcga gagacccctc gtcaacggaa agctcgttgc
      361 ccagtttctc tatctctctc gcgagcactt ccctgtcgcg gtcgtaggga aaggaaaagg
      421 gtatcgtcct tacgccccgg tagcgaagga tctcggcaag ggcctgcgtg ttgctgcagt
      481 cgccttccat aacggtgatg acgccggcca tatcctgctc catgatgacc ccgtagatgc
      541 ccttgatcca gttgcacatg ctcttcggga aaccgtcgcg ctccgcgcgt tcgatgaaac
      601 ggcccgggtc ggggtccgtg acgaagatgt tgttcaggtc gcagggctcg taccccgcgg
      661 cgaagatgag ctccacgggg atcgtggtgg taaaaccgat gatgcccttc gatgtcggtg
      721 atgatatcat gctcttaaag ctactatgtt ttgccgcgcc ctgcaaccgt ttcagcgggg
      781 aggtgctgtc cggcacggtc tttcgggtct catatataaa ctatgatatc ctgggcaaat
      841 atttttatag tcaacatatg catgaaataa ttcctcaggc gggcttgttg gcctttcgcc
      901 gggccagcgt cctgtcggca tagcgcagat catcggtcac cctcttccca gcggtgacga
      961 aaggcggtat cgaggtggga gactcggcga ggagcatcgt gagtccctca tgtcttcccc
     1021 cgtacctgtc cagttcatag tactggttgt tccagagaaa gcatatgcgc tccctggcga
     1081 ggacctccgt cttcgggtcc atgaggcggg caaggctgag gtactcctgc tcggggatga
     1141 gctcctcttc ctctatcgga agactgcggg cacccgcgtg cctcgtcctt gccagaaaat
     1201 ggaggtagac accatcctgc ccccttctgc gtatcctcgt cacctctctc ctgtccctcg
     1261 agcggagata cacctgctcc atatcgatcc tctgatgggc cggaagagcg gcgagatcga
     1321 catgcctgac gaggtatttc tcccccgtgg gaaggacgtc gggtatcccg agaaacctgg
     1381 ctattgccga aaaggccctc tttatcttcc cctcgaaatc ggtactgttg tcgacgatct
     1441 tgaaacgggg gtggccgagc cagctctcct tgatcctcag gtctatgcgc agcgcctctt
     1501 cgggtgtctc gagacgggcc gtgttgttct cccccgtgta atactcggcg gcgccgtccg
     1561 ccgccgtgac gagatggatg accccgtcgt agcgatggcg gatcgcggcc cgtgtcaaac
     1621 ccttcttctt caggatgctg ttaaagacgt cgtcggtgag aaaggccctg atgtccatga
     1681 tgcccctgtc gaggagtatg accttcctgc gctccgggaa tatcctcgat actgcctgtt
     1741 tgtacgtgtc ttcgaaggac atctgcatgt cgaagatggc ctcctcgaag accactatct
     1801 ggcccggttt atccatcttg cgcctgtcga tgccgtttcc cgtgatgagc gtcgccgtct
     1861 cggggacaac aaagaccatg aacccgtggt ctgaaagctt ctccgtaaga taggcaagcg
     1921 aggagctctt tcccgaacag ggccctcccg taagaacgat catgctgaca ggctgcaaca
     1981 ggcgggaact aacaggcata caacctctca aagatgggct tgaatgcttg agtcgcttga
     2041 gtgttagaaa caaagacaca atagtgatga cacgtagaat gcctggccgg gcaaagcaat
     2101 ctcttgcttt ttactctcaa gcgattcaag cattcaagcg attcaagccg tctttagctg
     2161 tttactatgt ttctccagtc cgtgacgacg gggtcgaagc cccttgacct gagcatggcc
     2221 ttgatctcgt cgaagctccg gggatcgaaa acctcgaact ggccgtcctc gtacttgtgt
     2281 tccccttcga ggtatcctcc cacacgcgtc gaggaacctg cagacatctt ggtgacgccg
     2341 aactcgagta tgctgtcccg gaaccggggc gtctccctgg tggagatgct gatccccacg
     2401 cgggggaaga agagtcgcgc tgcggtcatt atcttcagca tatccctgtc ggacacttcc
     2461 tggtactggt ggatatcgtc ggccacgcgc ctcaggcgcg gaaaggcaag accgaactcg
     2521 acaccgggat acctcttttc gaggccgcgg acgtgctcga agagcgcgat gacgtccctg
     2581 cgccagtccg taagccccag gagggccccc atcgatatgt gcctgatccc ggagc
//
LOCUS       DAOQTR010000324         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46807_length_2631_cov_4.143634, whole genome shotgun sequence.
ACCESSION   DAOQTR010000324 DAOQTR010000000
VERSION     DAOQTR010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46807_length_2631_cov_4.143634"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKN85_08010"
     CDS             complement(<1..999)
                     /locus_tag="PKN85_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNT44393.1"
                     /translation="MMLKRYLLAPGPTQIPPEVLLKMAEPMVHHRNPMFEVVVDEVRE
                     NLRHLFGTGNEVLIFSSSGTGAMEGAVTNMLSPGDRAVVVRSGKFGERWAEICRAYGV
                     ECVNIDLPWGDVLDPAVVERTLKENPDAKAVYMQATETSTGTMFPVREVAAIVRECPG
                     TLMVVDGITGVGVFPLPMDEWGIDVLIGGSQKALMLPPGLAFAGVSDKAWEFNKASRM
                     PRFYFDWEKERNNLKKNQTNFTPAISLIVGLRESLRILREEGLENVYRRSEMLAKATR
                     EGACAIGLEIFSKNPSPAVTAICAPEGIDGQAIYKTLWKKYGVTGAGGQDQLKGRIF"
     gene            1145..1540
                     /gene="nifU"
                     /locus_tag="PKN85_08015"
     CDS             1145..1540
                     /gene="nifU"
                     /locus_tag="PKN85_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HNT44394.1"
                     /translation="MAQGPYSDTVMDHFEHPRNMGEMDGADGMAQVGNPACGDVTRIF
                     LKIENDRIVDVKFKTFGCAAAIASSSMTTELIKGKTIDEALKLTNEIVARELGGLPEA
                     KMHCSVMAEEALRAAIEDYRKKQGEQGDP"
     gene            1537..1920
                     /locus_tag="PKN85_08020"
     CDS             1537..1920
                     /locus_tag="PKN85_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNT44395.1"
                     /translation="MTEFHVRTSKRIEALNITSQVVNAARGKAGKILHVYTPHTTCAI
                     TINEYADPDVVRDIIEALDRMVPAGYPYRHGEGNSDAHIKAVLVGASVSIPLSGDVPA
                     LGTWQGIFLMEFDGPRERKVQITVT"
     gene            1964..2428
                     /locus_tag="PKN85_08025"
     CDS             1964..2428
                     /locus_tag="PKN85_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44396.1"
                     /translation="MTRLKTICAITLCLVFLGASATIAFAKNEKVATPKEYGAYIKTS
                     KGLIRLLPNIVFDEDGVIFVESNNPAHFFLKDFQYFVLYGKYEMSVLTVNPMGFYQSS
                     ALGKPRFAFGKDIEIDVKPQGKDMYIIKPKGLLGRGYYSIWINDTAWDFMLD"
BASE COUNT          605 a          770 c          734 g          522 t
ORIGIN      
        1 gaatatcctc cccttcaact ggtcctgtcc accggcaccg gtcacaccat atttcttcca
       61 cagggtcttg tatatcgcct ggccgtcgat cccttccggg gcgcaaatgg ccgtgaccgc
      121 cggagaggga ttcttcgaga atatctccag accgatcgcg caggcgcctt cccgggtcgc
      181 tttggcgagc atctcactcc tgcggtagac attttccagg ccttcctccc tgaggatcct
      241 cagggactcc ctcaagccca cgatcagcga gatggcgggg gtgaagttgg tctggttctt
      301 tttcagattg ttccgttcct tctcccagtc aaagtagaac ctcggcatac gggacgcctt
      361 gttgaactcc caggccttgt cgctgacccc ggcaaaggcc agccccggcg ggagcataag
      421 ggccttctgg gaaccgccga tcaggacgtc gatgccccac tcgtccatgg gcagggggaa
      481 aaccccgaca ccggttatcc cgtcgacgac catcaatgtg ccggggcact ccctgacgat
      541 ggcggcaact tccctgacgg ggaacatggt ccccgtggag gtctccgtgg cctgcatgta
      601 gaccgccttg gcgtcggggt tctccttgag ggtcctttcg actaccgccg ggtcgaggac
      661 gtccccccag ggtagatcga tgttcacgca ttccacgccg taagcgcggc agatctcagc
      721 ccagcgttcc ccgaacttgc cgctgcggac cacgacggcc ctgtctcccg gtgaaagcat
      781 gttcgtcacg gcgccttcca tcgcgcccgt acccgatgaa gagaaaatga gcacctcgtt
      841 gccggtgccg aagaggtgcc tcaggttctc cctgacctca tccaccacca cctcgaacat
      901 tggattgcga tggtggacca tgggctccgc catcttcagc aacacctccg gcgggatctg
      961 ggtcgggccg ggagcgagca agtaccgttt aagcatcatc aatctcctta attttaatgc
     1021 tagtttctaa caaattcgtc ttaaaaccgt caagcaaaat gatattacaa aaggtctgcc
     1081 gtttatgcta tatttcacct atacgattcg tcccgccatc gcgggtgaac aacaggaggt
     1141 aatgatggcg cagggcccct atagcgatac agtgatggac catttcgagc atcccaggaa
     1201 catgggcgag atggacggag ccgatggcat ggcacaggtg ggcaaccccg cgtgcggtga
     1261 cgtgacgagg atcttcctca agatagagaa cgaccgcatt gtcgatgtca agttcaagac
     1321 cttcggctgc gcggccgcga tcgcgtcaag cagcatgacc acggaactca tcaagggaaa
     1381 gaccatcgat gaggctctta agctcacgaa cgagatcgtc gcgcgggagc tcggcgggct
     1441 ccccgaggcc aagatgcact gttcggtcat ggcggaagag gccctcaggg ccgccatcga
     1501 agactacagg aagaaacagg gggaacaggg cgatccatga cggagttcca tgtcagaacg
     1561 tcgaaaagaa tagaggccct gaacataacc agccaggtcg tgaacgcggc aaggggaaag
     1621 gccgggaaga tcctccatgt ctacaccccc cacaccacgt gcgcgattac gatcaatgaa
     1681 tacgctgacc ccgatgtcgt gcgtgacatc atcgaagccc tggacaggat ggttcctgcg
     1741 gggtatccgt accggcacgg ggagggaaat tccgatgccc acatcaaggc cgtgctcgtc
     1801 ggggcgtcgg tgagcatccc gctttccggg gacgtgcccg cgctgggaac ctggcaggga
     1861 atcttcctca tggaattcga cggaccgcgg gagagaaagg tccagatcac ggtgacatga
     1921 gatagcaaca cgcgtgccga aagaacgata aatggagaaa ccaatgacaa gactgaagac
     1981 aatatgcgcc ataacactgt gtctcgtttt cttaggggcc tctgcaacaa tagccttcgc
     2041 gaagaatgaa aaggtcgcga caccgaagga gtacggggca tacatcaaga cgagcaaggg
     2101 actcatcagg ctcctgccca acatcgtctt cgatgaggac ggggtcatct tcgtggagtc
     2161 caacaatccc gcccattttt tcctcaagga tttccagtac ttcgtcctct acggcaaata
     2221 cgaaatgagc gtgctcaccg tgaaccccat gggcttctat cagtcgtcgg cactcggcaa
     2281 accacgcttc gccttcggca aggacatcga gatcgacgtc aaaccccagg gcaaagacat
     2341 gtacatcatc aaacccaagg ggctgctcgg caggggatac tacagcatct ggatcaacga
     2401 caccgcatgg gatttcatgc tcgactgaca ccggcttgaa ccgcttgaat ggcttgagcc
     2461 gtttgagcgt tgaaagacaa aaaaaatgac ccccgttgtg ctcggccaca caggggtcgt
     2521 tctttttcta acgttcaagc ggctcaagcc attcaagcgg tttaagccgg tgttttttgg
     2581 aggcggcatc cggatttgaa ccggagaata acggttttgc agaccgtcgc c
//
LOCUS       DAOQTR010000325         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46933_length_2627_cov_3.452566, whole genome shotgun sequence.
ACCESSION   DAOQTR010000325 DAOQTR010000000
VERSION     DAOQTR010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46933_length_2627_cov_3.452566"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKN85_08030"
     CDS             <1..299
                     /locus_tag="PKN85_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT44397.1"
                     /translation="VLTTLRYFRNEYLEHIKDHRCRATVCKPLIEYRVIKEKCTGCQS
                     CVRVCPTGAITGPRSEPHNLDPSKCIKCRSCYEVCRYEAIAGDAIVIRTAESKS"
     gene            296..2494
                     /locus_tag="PKN85_08035"
     CDS             296..2494
                     /locus_tag="PKN85_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT44398.1"
                     /translation="MTREKKVTITIDNRKVEAKANMTILQAARENNIDIPSLCALEHL
                     PSYGACRLCVVEVDGIRGFPTSCTTPVEDGMVIRTDTAEVRTLRQEVLKLLLSEHPAS
                     CLFCEEQTDCKNFQGTIRKVGVTTGCRYCPNDDMCELQEITKKVGLTETSYPVYYRNF
                     PVEKEDPFYDRDYNLCLLCGRCVRVCNDIRLNGTLSFKQRGKLTTIGPAFGRTHLEAG
                     CEFCGACVAVCPTGALSAKTSKWAGKPDEIVESTCPYCPVGCTLDLKVKAGEVVDVSA
                     DYDSPTEHGLICVKGRFAIPEYVLSPDRLAQPTILKPEGYDLLPWEGALDEAAAKLKA
                     AGPKGSFIVVSPQLSNEDLFVAQRFAREVLGTEAILSPVMFDLGSDLRPFLDLAVLSE
                     SVDTIDTAAGILTIGLDTTYGFTPVGIAAKRAAKRGAVLVTVSTDECNLDMLSEEAVQ
                     SDPAKWPDFMEGVMKSMSGNGTRKKAASSFFKKGDRNVLVIGPDAIFSARRGEIFESA
                     LTMRDSLGWKVIVCHPYTNLMGMLAMGAFPGLKPGGMRSDGALKLKDPAAVIDVQQKW
                     KVGYFIGEVPSPQMPGCDYIIYENALPAPSGIDPDLVLPAALFTEAPGTTISCEGKIL
                     VFNKASAPFADSKPDWWIMSSIAERMKKGALKYASLAAIQSEIRKSIPGFSGKDRRVP
                     RKKVAMEGKAAAARNPHGAGRVMTPLETFRGVALHDHVAGMKAIEERRRK"
BASE COUNT          559 a          791 c          810 g          467 t
ORIGIN      
        1 cggtcctcac gactctgagg tatttccgca acgagtacct ggagcacatc aaggaccaca
       61 ggtgccgcgc caccgtgtgc aagccgctca tcgaataccg cgttatcaag gaaaaatgca
      121 ccggatgtca atcgtgtgtc cgggtttgcc caacgggggc aataacaggt ccgaggtcgg
      181 aaccgcacaa cctcgatccg tccaagtgca tcaagtgccg ttcttgctat gaggtctgcc
      241 gttacgaagc tatcgcgggc gacgccatcg tgatcaggac agcggagagt aaatcatgac
      301 cagggagaaa aaggtaacca tcacaatcga caacaggaaa gtcgaagcaa aggcaaacat
      361 gaccatcctc caggcggcgc gggagaacaa catcgacata ccctcgcttt gcgctctgga
      421 gcatctgccg tcctacggcg catgccgcct gtgcgttgtg gaggttgacg ggatacgggg
      481 ttttccgacg tcctgcacca caccggtgga ggacggcatg gtgatccgca ccgacacggc
      541 ggaggtgagg acgctccggc aggaggtcct gaaactcctc ttgagcgagc atccggcaag
      601 ttgcctgttc tgcgaggaac agacggactg taagaacttc caggggacga tccgcaaggt
      661 cggtgtcacc accggctgcc gctattgtcc caacgacgat atgtgcgagc tccaggagat
      721 aacaaagaaa gtggggctca cggagacgtc ctacccggtc tattacagga acttccccgt
      781 cgaaaaggaa gaccctttct acgacaggga ctacaacctg tgcctcctgt gtggacgctg
      841 tgtcagggtc tgcaacgaca tacgcctgaa cgggaccctg agcttcaagc agcgcggaaa
      901 gctgaccacc atagggccag ccttcggacg cacccatctc gaggcgggct gcgagttctg
      961 cggggcctgc gtcgccgtgt gcccgacggg cgcgctgtcg gccaagacca gcaagtgggc
     1021 gggcaaaccc gacgagatcg tggaatccac ctgtccctac tgtcccgtcg gctgtaccct
     1081 cgacctcaag gtgaaggcgg gagaggttgt cgacgtcagc gccgattacg attcgccgac
     1141 cgagcacggg ctcatctgtg tgaaggggcg cttcgcgatc cccgagtacg tcctgagccc
     1201 cgacaggctc gcgcagccga cgatactcaa gcccgagggg tacgatctct tgccctggga
     1261 gggcgccctt gacgaagccg cggcgaagct caaggcggcc ggcccgaagg ggtccttcat
     1321 cgttgtctca ccccagcttt ccaatgagga ccttttcgtg gcccagcgct tcgcgagaga
     1381 ggtcctgggg acggaggcga tcctctcgcc cgtcatgttc gatctcggca gtgacctgag
     1441 gcctttcctc gaccttgccg tgctctcgga atccgtggac accatagaca cggcggcggg
     1501 catcctgacc atcggcctcg acaccacgta cggattcacc cccgtgggca ttgccgccaa
     1561 gagggcggcc aagcggggcg ccgtcctggt caccgtaagc accgacgaat gcaatctcga
     1621 catgctctcc gaagaggccg tccagtccga cccggcaaag tggcccgact tcatggaggg
     1681 tgtcatgaag tccatgtccg gcaatgggac ccggaagaag gcggcatcct ccttcttcaa
     1741 gaagggcgac aggaacgttc tcgtcatcgg gcccgacgct atattctcgg ccaggcgcgg
     1801 ggagattttc gagagcgccc tcacgatgag ggacagcctc ggatggaagg tgatcgtatg
     1861 ccatccctat acgaacctca tgggcatgct tgccatgggc gcctttcccg gtctgaaacc
     1921 gggaggtatg cggtcggacg gggcgctcaa gctcaaagac ccggcggccg tcatcgacgt
     1981 gcagcagaaa tggaaggtcg gttatttcat tggcgaggtg ccctctcctc agatgcccgg
     2041 gtgcgattac atcatctatg agaacgccct gcccgcgcct tcggggatcg atcccgacct
     2101 cgtccttccg gcggccctgt tcacggaggc tccggggaca acgataagct gtgagggcaa
     2161 gatcctcgtt ttcaacaagg cgtcggctcc ctttgccgac tcaaagcctg actggtggat
     2221 catgtcgtcc atagcggaaa ggatgaagaa gggcgccctg aaatacgctt ctctcgcggc
     2281 catacaatcg gagatcagga agtccattcc cggcttttcg ggcaaggacc gacgggtccc
     2341 aaggaaaaag gttgccatgg aaggcaaggc ggccgcggca cgcaaccccc atggggcggg
     2401 ccgggtgatg accccccttg agaccttccg cggtgtggcc cttcacgatc atgtggccgg
     2461 catgaaggcg atagaagaaa ggaggcgcaa gtgaagaagg tcatagaacg gtcgatgatc
     2521 gtccccaata tgcatctgct cgtagtcgag gcacccgaga tcgcctccaa ggtgaagccc
     2581 ggacagttcg tcattgtccg gggcggcgac gaaggcgagc gcatccc
//
LOCUS       DAOQTR010000326         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_46979_length_2625_cov_4.159922, whole genome shotgun sequence.
ACCESSION   DAOQTR010000326 DAOQTR010000000
VERSION     DAOQTR010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46979_length_2625_cov_4.159922"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..530)
                     /gene="cutA"
                     /locus_tag="PKN85_08040"
     CDS             complement(213..530)
                     /gene="cutA"
                     /locus_tag="PKN85_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNT44399.1"
                     /translation="MPEIVQIVTTIDDREAAGRLGRRLVEERLVACCQVVGPIRSIYR
                     WKGTVEETDEWYCVMKTRPSLYRQVEEAIRRLHPYEVPEIVATPVSSALPDYARWVET
                     ETS"
     gene            674..1264
                     /locus_tag="PKN85_08045"
     CDS             674..1264
                     /locus_tag="PKN85_08045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNT44400.1"
                     /translation="MFQINDGNAIILASESTRRVDILRTLGIPFSIIPPDIDETRKLE
                     ETPREYALRLSYEKAHKVGRHFPDKWVIGADTIVVYKNRILGKPVNENEAFKTLQLLK
                     GKWHKVITGFCVLNVDREITCHDAVETRVFMKDLVDEEIMNYIRTSEPLDKAGSYAVQ
                     GKGGYMVKEIKGSYSNVVGLPICEVAEVLLSLGILS"
     gene            1261..1935
                     /locus_tag="PKN85_08050"
     CDS             1261..1935
                     /locus_tag="PKN85_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNT44401.1"
                     /translation="MSDIRHALGVVRERIDRACATAGRDPGEVTLVAVTKTVDASRVI
                     EAIEAGVGDIGENYVQEAKGKIKTIGPGRARWHMIGHIQSNKVKYIPALFDCVHTVDS
                     TAILEGLERRGKDMDVLFEVNLAGEATKSGTDVEGLRRILERASSLVHIKPVGLMTMP
                     PYVEEPEEVRGVFGQLRTTLEMMNAEFGLAMKELSMGMSSDFEVAVEEGATMVRIGTA
                     LFGERR"
     gene            1932..>2625
                     /locus_tag="PKN85_08055"
     CDS             1932..>2625
                     /locus_tag="PKN85_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNT44402.1"
                     /translation="MKPWGGRFKGGTDPLLERFSASIDVDRRLYRQDIEGSRAQAEML
                     ARIGVLTAAERKKVLKGLDEIEEEITAGSFTFTDALEDIHMHIEARLIEKTGEAGKKL
                     HTGRSRNDQVSLDMHLFLKEELRAIDGCVAALLRELLAKAEREKKVIMPGYTHLQRAQ
                     VVPFSHYLLGHYYSLKRDRARLAAALAMADVLPLGSGALAGSTIPLDREWVRGRLGFS
                     RVSENSMDTVADR"
BASE COUNT          593 a          718 c          829 g          485 t
ORIGIN      
        1 tggaaggata cggatcccgc gcgagtgcaa caacatcctt cagatttaag ctttcccaca
       61 taacccctcc aagaatgatg aaaaatttca ctcattatat gaaggttcac ctttattgtc
      121 aaggtaagtt tgggacgccg taacccctcc aaaacaaaga cagacaggca ttccgtgaag
      181 gatccgtcgt cgcggccgaa agggccgtcc cgtcaggagg tctccgtctc cacccagcgc
      241 gcgtagtccg gcagagcgga gctcacgggg gtggcgacga tctccggaac ctcgtaaggg
      301 tgaaggcggc gtatcgcctc ttccacctgg cggtagaggg acggtctcgt cttcatgacg
      361 cagtaccatt cgtcggtctc ttccacggtt cccttccacc ggtagatgct ccggatgggg
      421 ccgacgacct ggcagcaggc gacgagcctt tcctcgacga ggcgccttcc cagtcgcccg
      481 gctgcctccc ggtcatcgat ggttgtcact atctggacga tctcgggcat gtcttcctcc
      541 tcaaaagcgg gtcccgccgt cagcgtgcgc cggtggcctc gcgggaacaa tttaacttga
      601 aaatatggga gatttcctat atttttctat gctacaatag cttttacagg tagtacagca
      661 atttgaggtt gccatgttcc agataaacga cggaaacgcc atcatactcg caagcgaatc
      721 gacgaggagg gtcgacatcc tgaggaccct ggggattccc ttttcgataa tcccgcccga
      781 tatcgacgaa acgaggaagc tcgaagagac accccgcgaa tatgccttga ggctttccta
      841 cgagaaggcg cacaaggtgg gaaggcattt tccggacaaa tgggtcatcg gggccgacac
      901 gatagtggtc tacaagaaca ggatcctcgg caagcccgtg aacgagaacg aggccttcaa
      961 aacactgcag ctccttaagg ggaaatggca caaggtcatt accgggttct gcgtcctgaa
     1021 cgtggacagg gagatcacct gccacgacgc cgtggagacg agggtcttca tgaaggacct
     1081 tgtcgacgag gagataatga actatatccg cacctcggag ccgcttgaca aggccggctc
     1141 ctacgcggtc cagggcaagg gcggctacat ggtgaaggag ataaagggct cctactcgaa
     1201 cgtcgtgggc ctcccgatct gcgaagtcgc ggaggttctc ctctcgctgg gcatcctttc
     1261 atgagtgaca tccggcacgc cctcggggtc gtgcgcgaga gaatagacag ggcctgcgcc
     1321 accgcgggaa gggacccggg cgaggtgacc ctcgtggcgg tgacgaagac cgtcgacgcc
     1381 tcccgggtga tcgaagcgat cgaagcgggg gtgggggaca tcggcgagaa ttacgtgcag
     1441 gaagcaaagg ggaagataaa gactatcggc cccgggcgtg cccgctggca catgataggg
     1501 cacatccaga gcaacaaggt gaaatacatc ccggcactct tcgactgcgt ccacaccgtc
     1561 gacagcaccg ccatactgga agggctggaa aggcgtggga aagacatgga cgttctcttt
     1621 gaagtgaacc tggcggggga ggcgacgaag agcgggaccg acgtggaggg tttgaggcgg
     1681 atcctggaaa gggcttcgtc gctcgtgcac attaagcccg tgggtctcat gacgatgccg
     1741 ccttacgtgg aagagcccga agaggtgcgc ggtgtattcg ggcagttgag aacgaccctt
     1801 gaaatgatga acgccgagtt cggccttgcc atgaaagagc tgtccatggg gatgtcttcc
     1861 gatttcgagg tggccgtcga ggaaggagcg accatggtga ggataggcac ggcgttgttc
     1921 ggggagagac gatgaaacca tggggtggaa ggttcaaggg aggcaccgac ccgcttctag
     1981 agaggttcag cgcgtccata gacgtggacc gccggctcta tcgccaggac atcgagggaa
     2041 gcagggccca ggcggagatg ctggcgagga tcggggtcct taccgccgcg gaaaggaaga
     2101 aggttttgaa aggtctcgat gagatcgaag aggagataac ggccggttcc ttcacgttca
     2161 ccgatgccct cgaagacatc catatgcaca tcgaggcgcg actcatcgaa aagacgggtg
     2221 aggcggggaa aaagctgcac acgggacgca gccgcaacga tcaggtctcc ctggacatgc
     2281 accttttcct gaaggaagaa ctccgcgcca tcgacgggtg cgtcgcggcc cttctgagag
     2341 agctcctcgc gaaggccgag agggagaaga aggtcatcat gcccggttat acgcatcttc
     2401 agagggccca ggtcgtgcct ttttcccact accttctcgg ccactattac agtttgaagc
     2461 gcgacagggc gagactggcg gcggcgctcg ctatggccga tgtcctgccg ctcggcagcg
     2521 gagcgcttgc cggctcgacg atacctctcg acagggagtg ggtcaggggc cggctcggtt
     2581 tctcccgcgt ctccgagaac agcatggaca ccgtcgccga caggg
//
LOCUS       DAOQTR010000327         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_47259_length_2616_cov_3.189379, whole genome shotgun sequence.
ACCESSION   DAOQTR010000327 DAOQTR010000000
VERSION     DAOQTR010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47259_length_2616_cov_3.189379"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PKN85_08060"
     CDS             <1..1313
                     /locus_tag="PKN85_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT44403.1"
                     /translation="QKMIPVLEKAGLAYFNVQAGWHESPIPLVQQWVPEGAFVYLGEA
                     IKKVATVPVAVGYRLKDPIMAEEIVAAGKVDMIAMARQLIADADWANKAQAGKLDDIR
                     KCITCCRCMDDNFVGVPITCSVNTNLEGLPETPVEKPKKVMVIGGGPSGLEAARVATI
                     RGHKVTVFEKGKRLGGLTTLASVLNSEIEPFHDWLVKQVKDLNIEVKLGTEVKADTVD
                     AFKPDVVILAAGGSPAELNVPGIDSDNVISSHDIEAFVSGQSTKKGLMWTLAAKVGKD
                     VAGSPALMRKMLGLNFPIKKRVAIIGGQFSGCELALTLMEKGKQVRIIEETKRLGADI
                     GPVTRWVEMDMMKKGGVAMETLTKVKEITDKGVKAVREDGTEVFFDADTVLLALGLKE
                     NMDLADKLKGKVAEVYVVGESAGGGGKKRLREAMNSGRDVGAKI"
     gene            1390..2250
                     /locus_tag="PKN85_08065"
     CDS             1390..2250
                     /locus_tag="PKN85_08065"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNT44404.1"
                     /translation="MKIKKVGVVGCGFMGGGIVQVCAAAGYDVVTTDISDDAVTKGIA
                     AIKDRLAKKVKKSEMTQQQMDDSMGRITGSTDINVYRDCDLVIESVFEDMELKKRLFA
                     ELDKVCEAKTYFGTNTSCLPVIDMAMATSRPEKVVGIHFFTPPPLMPLVEIMESVKTS
                     KETMDMAVEFGKSVDKSVIVAKDTPGFIVNAQMIPNVLNAVRMVESGIATAEDIDLGA
                     VKGLSHPIGPIALADFFGLDVLYAISTHMYEETKDPMVKPPLILKRMVMARMLGRKTG
                     KGFYDYTSKG"
BASE COUNT          643 a          671 c          845 g          457 t
ORIGIN      
        1 tgcagaagat gatccccgtg ctggaaaagg ccggtcttgc ctacttcaac gtccaggccg
       61 gctggcacga aagcccgatc ccgctcgttc agcagtgggt tccggaagga gcttttgtct
      121 atctgggtga ggccatcaag aaggtggcaa cggtccccgt cgccgtgggg tacagactga
      181 aagacccgat catggcggaa gagatcgtgg ccgccggcaa ggtcgacatg atcgccatgg
      241 cgcgtcagct catcgccgat gccgactggg ccaacaaggc tcaggcaggt aagctcgacg
      301 acatcaggaa atgcatcacc tgctgccgtt gcatggacga caacttcgtc ggcgtgccca
      361 tcacctgcag tgtgaatacg aacctcgagg gcctccccga gacaccggta gagaagccga
      421 agaaggtcat ggtgatcgga ggcggtcctt ccggtctgga agccgcacgg gtggccacca
      481 tcaggggcca caaggtgacg gtgttcgaga agggaaaacg gcttggcgga ctcacgaccc
      541 tggcctcagt gctcaactcg gagatcgagc ctttccatga ctggctggtg aagcaggtga
      601 aggacctcaa catcgaggtg aaactgggaa cagaggtcaa ggccgacacg gtcgacgctt
      661 tcaagcctga cgtggtcatc ctggcggccg gcgggtcccc cgccgagctc aatgttcccg
      721 gcatagacag cgacaacgtg atctcaagcc acgacattga agccttcgtg agcggtcaga
      781 gcacgaagaa gggcctcatg tggacccttg cggcaaaggt cggaaaagac gtcgccggca
      841 gcccggcgct catgcggaag atgctgggtc tcaacttccc gataaagaag cgggtcgcca
      901 tcatcggcgg gcagttctcc ggttgcgagc tcgcgctcac cctcatggag aagggaaagc
      961 aggtgcggat catagaagag acgaaacgtc tcggcgccga tatcggcccc gtcacccggt
     1021 gggtcgagat ggatatgatg aaaaaaggcg gcgtcgccat ggagaccctg acgaaggtga
     1081 aggagatcac cgacaagggt gtcaaggcgg tcagggagga cgggaccgag gtcttcttcg
     1141 acgccgacac ggtccttctg gcccttggcc tgaaagagaa catggatctc gccgacaagc
     1201 tgaagggcaa ggttgccgag gtgtacgtcg tcggtgaaag cgccggcgga ggaggaaaga
     1261 agaggctcag agaggccatg aactccggcc gcgacgtggg cgccaaaata taagtgatta
     1321 acagccgctt ccggcgtgcc gcaaggcgtg acacgccgga ggacggttgc tcggaaaaag
     1381 gagtagactg tgaagatcaa gaaagttgga gtggtaggct gcgggttcat gggtggaggc
     1441 atcgttcagg tatgcgccgc ggctggatac gatgtggtga ccacggatat ctccgatgac
     1501 gcggtcacga aagggatagc cgccataaag gacaggctgg ccaagaaggt gaagaagtcg
     1561 gaaatgacgc agcagcagat ggatgactcg atgggccgta tcacggggtc caccgacatc
     1621 aacgtctaca gggactgcga tctcgtcata gagtccgttt tcgaggacat ggaactcaag
     1681 aagaggcttt tcgcggagtt ggacaaggtg tgcgaggcga agacgtattt cgggacgaac
     1741 acctcctgcc ttccggtgat cgacatggcg atggccacct cacggccgga gaaggttgtg
     1801 ggtatacact tctttacccc tccgcctctt atgccccttg tggagatcat ggaaagtgtc
     1861 aagacaagca aggaaaccat ggatatggcc gttgagttcg gcaagagcgt ggacaagagc
     1921 gtgatcgtcg cgaaagatac cccgggcttc atcgtcaatg cccagatgat acccaatgtc
     1981 ctgaacgccg tacgcatggt cgagagcggt atagcgacgg cggaagacat cgacctgggt
     2041 gccgtaaagg gtctttcgca tccgatcggg cccatagcgc tggcggattt cttcggtctc
     2101 gatgtgctct acgcgatcag cacgcacatg tatgaagaaa cgaaggatcc gatggtgaag
     2161 cccccgctga tattgaagcg catggtcatg gccaggatgc ttggccgcaa gacgggaaag
     2221 ggcttctacg actatacatc gaagggatga atgaatggcc tgcggcggtg atgcggtgac
     2281 gcatgccgga cgacgggctg gaagatcatg atgaaaagta ttgaaatagc gagagataaa
     2341 acgagccggt ccgtgacccc gaaaaaacct tcctactccc accttaacgg gccggcttcg
     2401 ctatttttca tgacaggtcc cggagactga gggaagacgg caaggacatc gtggacgtca
     2461 ggatatctgc caatagaagg gaaagaagca tcggtgaaga cgttccctga agaacgcata
     2521 tttggacatc gcctccctgc agcagcatag gcggcaaccg agatcgagga ggacaacatg
     2581 agtaacgagg gtacacagaa ggcttacagc ggatgc
//
LOCUS       DAOQTR010000328         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_47279_length_2615_cov_4.882031, whole genome shotgun sequence.
ACCESSION   DAOQTR010000328 DAOQTR010000000
VERSION     DAOQTR010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47279_length_2615_cov_4.882031"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PKN85_08070"
     CDS             <1..811
                     /locus_tag="PKN85_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT44405.1"
                     /translation="QRRDLEAKRAQDLYNQTNYEREQRLLAKEYTTKDSVEKAKRELD
                     VSRAQVELGKAKVREAQEKLKALKVSHSYTKIYAPISGYISSVATQEGETVVSGLSAP
                     NLITIIDPTKLEMWIYVDETDIGRVKPGLKVEYWVDAYRDKLYRGVIDRIYPQPEIKE
                     NIVYYLAIVKIDPNDALTLKPEMTSHVRIVIEEKANVIVVPNGAIRFEDRKNVVYVRS
                     KGKTIRQEIVPGVRDQSYTEIISGLTEGQQVVVPSMAPAANKPQNNAGPRK"
     gene            808..1482
                     /locus_tag="PKN85_08075"
     CDS             808..1482
                     /locus_tag="PKN85_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44406.1"
                     /translation="MIVLKDIRKSYFVGDRELPILKGIDLTVTKGEFVVLMGVSGSGK
                     TTLMNIIGLLDKQTEGEYIFADTNVDGLDDEKLADMRNNHIGFVFQQFFLLPYLDAIE
                     NVLIPIVYSRKDIKHPRDKAKVLLGRFGLEHRIHNKPSQLSGGEQQRVAIARALVNDP
                     DLILADEPTGALDSKTGGEIMELFRMLNDEGKTIIVVTHDPKLASFGRRLVRIEDGAI
                     SEDIAT"
     gene            1451..>2615
                     /locus_tag="PKN85_08080"
     CDS             1451..>2615
                     /locus_tag="PKN85_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT44407.1"
                     /translation="MALFQKISQLREFVEEVLKILYYYRGRVIFSFSGVALGILSICI
                     IITTIDGANKKAQVIFEALGPDAIMIFGGGERQRAARIRVSTLRTGDADLLARVEGVY
                     DVMKVFSARNVMMRYKDRNWQTQAIGSTTNYFESFSWGFQAGTVFTADDYDRAEAVCV
                     IGAKVHEELFRGGPALGKTILVGKLPTRVIGVLEERGGSVGGPHVDDRVIMPLTTVTK
                     RIVNERRYLSMVRLKTHRDLDKTIEDVRATLRASHGLKGNLDDDFTIRSSKDILAFVT
                     VISGSLLLFLGTASIVALVVSGFVLANLFYLTIQERKKDIGIRRAYGATQKGILLSFL
                     FESILITFIGGIAGIVLSIVLGGTFEKLFDIPMMFSFKVVIFALLFSFLTGLLS"
BASE COUNT          601 a          734 c          777 g          503 t
ORIGIN      
        1 gcagcgcagg gacctcgagg cgaaacgcgc gcaggacctc tacaaccaga cgaactacga
       61 aagggagcag cgtcttctcg cgaaggaata caccacaaaa gacagcgtgg aaaaggccaa
      121 gcgcgagctc gacgtttccc gcgcccaggt ggagctcggc aaggcgaagg tgagggaagc
      181 ccaggagaag ttgaaggcgc tcaaggtgag ccacagctac acgaagatct atgcccccat
      241 atcggggtac atctcgtccg tcgctaccca ggaaggggag acggtggtct cgggcctgtc
      301 cgcgccgaac ctgattacca tcatcgaccc cacgaagctg gagatgtgga tatacgtgga
      361 tgagaccgac atcggaaggg tgaagccggg gctcaaggtg gagtactggg tcgacgctta
      421 ccgcgacaag ttatacaggg gcgtcataga ccgcatctat ccccagccgg agatcaagga
      481 aaatatcgtc tactacctcg ccatcgtcaa gatcgacccc aacgacgccc tcaccctgaa
      541 gccggagatg acaagtcacg tccggatcgt catcgaggaa aaggccaatg tgatcgtcgt
      601 gcccaacggg gcgatacgct ttgaggacag aaagaatgtc gtctacgtga gatcgaaggg
      661 caagaccatc cgccaggaga tcgttcccgg cgtgcgcgac cagagctaca ctgagataat
      721 atccgggctc accgaggggc agcaggtcgt cgtcccatcc atggctccgg ccgcgaacaa
      781 gccgcagaac aacgcgggcc caagaaagtg atcgttctca aggacatcag gaagagctac
      841 ttcgtggggg accgtgagct tcccatcctc aagggcatcg acctcactgt gacaaagggc
      901 gagttcgtcg ttctcatggg tgtctccgga tctggcaaga cgacgctcat gaacatcatc
      961 gggcttctcg acaagcagac tgaaggcgag tatatcttcg ccgataccaa tgtggacggc
     1021 ctcgacgatg aaaaacttgc cgatatgcgg aacaaccaca tcggtttcgt cttccagcag
     1081 ttcttcctcc tcccttatct cgacgccatt gaaaacgttc tcataccgat agtgtattcc
     1141 agaaaggata tcaagcaccc ccgggacaag gcgaaggtac tgctcgggag gttcggtctg
     1201 gaacacagga tacacaacaa accatcgcag ctctccgggg gcgagcagca gcgcgtggcc
     1261 atagcgcggg cactcgtcaa cgacccggac ctcatcctcg ccgacgagcc cacgggggcc
     1321 ctcgattcga agaccggcgg cgagatcatg gagctgttcc ggatgctcaa cgacgagggg
     1381 aagacgatca tcgtcgtgac ccacgatccc aagctcgcga gctttggaag acgccttgtc
     1441 aggatagagg atggcgctat ttcagaagat atcgcaactt agagagtttg tcgaagaggt
     1501 cctgaagatc ctttactact accggggcag ggtcatcttc tccttctcgg gcgttgcact
     1561 cggcatcctc tccatctgca tcataattac caccatcgac ggggccaaca agaaggccca
     1621 ggtcattttc gaggccctgg ggcccgatgc gatcatgatc ttcggcggcg gcgagaggca
     1681 gcgggccgcg aggatacggg tgagtacgct caggaccggc gacgccgatc tcctcgccag
     1741 ggtggagggc gtctatgatg tcatgaaggt gttttcggcc cgcaatgtca tgatgcggta
     1801 caaggacagg aactggcaga cccaggcgat agggtccacg acaaactatt tcgaatcctt
     1861 ctcctggggc tttcaggccg gcaccgtctt tacggccgac gactatgacc gggccgaggc
     1921 tgtctgcgtc atcggggcca aggtgcatga agagcttttc aggggcgggc ctgccctggg
     1981 aaagacgatc ctcgtgggca agcttcccac cagggtcatc ggtgtgctgg aggagcgggg
     2041 ggggagtgtc gggggacccc atgtggacga cagggtgatc atgcccctga caacggtgac
     2101 aaagcgtatc gtaaacgaaa gacggtacct gagcatggtg cggctgaaga cgcacaggga
     2161 cctcgacaaa accatagagg acgtgcgggc aacactgagg gccagccatg gtctgaaagg
     2221 taacctcgat gacgatttca ccatcaggag ttcaaaggac atccttgcct tcgtgacggt
     2281 gatctcggga tcgctccttc tctttctggg gacggcgtcg atcgtggccc tcgtggtgag
     2341 cggtttcgtc cttgccaacc tcttttatct cacgatacag gaacgcaaga aggacattgg
     2401 catcaggagg gcctatggcg cgacgcagaa gggcatcctc ctttctttcc tctttgagtc
     2461 gatcctcatc accttcatcg ggggcatcgc gggcatagtg ctcagcatcg ttctcggcgg
     2521 caccttcgag aagctcttcg acattcccat gatgttctcg ttcaaggtgg ttatttttgc
     2581 ccttcttttc agttttctca cgggcctctt gtcgg
//
LOCUS       DAOQTR010000329         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_47758_length_2599_cov_3.867531, whole genome shotgun sequence.
ACCESSION   DAOQTR010000329 DAOQTR010000000
VERSION     DAOQTR010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47758_length_2599_cov_3.867531"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /gene="dnaG"
                     /locus_tag="PKN85_08085"
     CDS             <1..1587
                     /gene="dnaG"
                     /locus_tag="PKN85_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNT44408.1"
                     /translation="PSFTVSVEKQIFYCFGCREGGNAINFIMKYENLTFVEALEHLGR
                     QYGIEVERRGDRRKAGHYDALGKLCEYYQKGLKSTSFALEYLARRGISREIADEFRLG
                     YSVRSRGALKAALKATEIPADVFLSTGVVRMKETDLYDMFGGRIVIPIIDVNKRVIGF
                     GGRTLEKDGIPKYVNSPESSVFSKRTSLFGIDRTKKHITDRDEVYIVEGYFDLIGLYG
                     AGITNAVSTLGTSVTEGQIMKLRNYTENITLMLDGDEAGIKSALRLIGLFAEMDVNGN
                     MVVLPEGHDPDSFVREKGAAAVAEVVKSKKPILDFYFDHYSGKEKMTTLQGKQAFIRQ
                     VMPHIDAIRDRVKRRLYVKRLSELTGVEEEHFAGTRTYEVKGGAPRADAVKGVIERRV
                     INVCMARPELLEYLKGKEVLRYIRDDDVREVMTRMVTCFEQGKNLDVKNFIETLDKED
                     LKGLVLKAAFDEAGTDPDEPEKVLLDYFRHIERQFFREKSKKITEKLAEAERSGDAAA
                     VTELLEQKRQVLTHIKNNFL"
     gene            1775..1897
                     /locus_tag="PKN85_08090"
                     /pseudo
     CDS             1775..1897
                     /locus_tag="PKN85_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            2396..2599
                     /locus_tag="PKN85_08095"
                     /pseudo
     CDS             2396..2599
                     /locus_tag="PKN85_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          700 a          640 c          809 g          450 t
ORIGIN      
        1 ccctccttta ccgtaagcgt cgagaagcag atcttctatt gtttcggctg cagggaaggc
       61 ggcaatgcca tcaacttcat catgaaatac gagaacctga ccttcgtcga ggccctcgag
      121 catctcggcc gtcaatacgg catagaggtc gaaaggagag gggacaggag gaaggcaggc
      181 cactacgacg ccctggggaa gctctgcgaa tactaccaga aaggccttaa gagcacgagc
      241 ttcgccctcg agtatctcgc gaggcgcggc atctcccggg agatcgcgga cgagttcagg
      301 cttgggtaca gcgtgcgttc ccgcggcgcc ttgaaggcag ctctcaaggc gacggagata
      361 cccgccgatg tgttcctcag caccggcgtc gtgaggatga aggagacgga tctctacgac
      421 atgttcgggg gcaggatcgt catccccatc atcgatgtca acaagagggt catcggcttc
      481 ggagggagga cactggaaaa ggacggcata ccgaagtatg tgaactcccc tgaatcatcc
      541 gtgttctcga agcgcacgtc cctcttcggc atagaccgga cgaagaagca cataaccgat
      601 cgggatgaag tgtacatcgt tgagggctac ttcgatctca taggcctgta cggggctggg
      661 atcaccaacg ctgtctcgac gctgggtacc tccgtcaccg agggacagat catgaagttg
      721 aggaattaca cggagaacat caccctcatg ctcgatggcg acgaagcggg catcaagagc
      781 gcgctgaggc tcataggcct tttcgcggag atggacgtca acggcaacat ggtcgtcttg
      841 ccggaaggtc acgaccccga cagctttgtg cgggaaaaag gcgccgccgc cgtggcggag
      901 gtcgtaaaaa gcaagaagcc catcctcgac ttctatttcg accattattc cggaaaagaa
      961 aagatgacga cccttcaggg caagcaggcc ttcatcaggc aggtgatgcc gcacatcgac
     1021 gccatccgcg acagggtcaa gagaaggctg tacgtcaaga ggctttcgga gttgacgggg
     1081 gtcgaggagg agcattttgc agggacgagg acgtacgagg tgaaaggtgg ggctccgcgg
     1141 gcggatgcgg tgaaaggggt gatcgagaga agggtcatca atgtctgcat ggcacgcccc
     1201 gagcttcttg aatatctcaa gggaaaggag gtattgcgct acatcaggga tgacgatgtc
     1261 agagaggtca tgacacgaat ggttacctgt tttgaacagg gaaagaacct tgacgtgaag
     1321 aacttcatcg agacgcttga caaggaggac ctgaaggggc ttgtcctcaa ggcggccttt
     1381 gacgaggccg ggacggaccc cgatgagccc gagaaggtgc tcctcgatta tttcaggcac
     1441 attgaacgcc agttcttcag ggagaagtcg aagaagatca ccgaaaagct tgccgaggcg
     1501 gagagatcgg gggacgccgc ggcggtcaca gaactcctgg aacagaagag gcaggtattg
     1561 acgcatataa aaaataactt tttatagaga ggtggccatg cctgaaaaag taaggagata
     1621 ttatccttat gaagagaatc ttgagaacgg ggagctgtac gcattcatat cacacggtga
     1681 gaagcccagt aacatccttg atgacatcga tctcttcgag atcgaacacc aggacgcgat
     1741 cggcggcgtg gagaacgtgg atgccgacga gcccatggag ggggatttcg aggaggtcta
     1801 cgatgatgac ctcatcaatc ccatccgtca ttacatcaag gagatgggaa gcatggccct
     1861 cctgacgcgg gaaggcgaaa aggagatcgc ccgtctcatg gaagaggcga gggaagatgt
     1921 aaagcaagtg ctcctgtcct ttcccggtac cgtcaaggaa ctcctcaatg ccctcatggc
     1981 gctcaagaca tcgaaggcgg ccgtcaaaga catcaccgtc gaggcggatg atgaggaaga
     2041 aggagacacg gagctcgaat atcagcggga aagggtgatc gccctcctcg agcggctcaa
     2101 ggaagagcat gcccggtcga gggagaacgc gaagggccgg aaggacggcc agggcaagga
     2161 gatgcagaag ataataaccg agatcaacct cagcaagaag atcacggaaa agatcattct
     2221 gcgcatgaag cggtacgtgg agcgcatgga caagatcgat gccgagatcg cccggtacaa
     2281 gaggtcaaaa gaaaagggaa gcaagaggca cctccaggcc ctgacgaaca gaaaggcgag
     2341 gatcgaggat gagatcggga tatcctcgaa actcctgagg cattacctga agaggatcga
     2401 gaaggcggaa tccgatttcg cgggggccaa gaacgaactc gtcaaggcca atctcaggct
     2461 tgtcgtgagc atcgcgaaaa ggtacataaa cagaggcctg tcactcctcg acctcatcca
     2521 ggaggggaac atcggcctga tgaaggcggt ggaccgcttc gaatacaaac ggggctacaa
     2581 gttcagcacc tacgcgacc
//
LOCUS       DAOQTR010000330         2589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_48042_length_2589_cov_3.557616, whole genome shotgun sequence.
ACCESSION   DAOQTR010000330 DAOQTR010000000
VERSION     DAOQTR010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48042_length_2589_cov_3.557616"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..1097)
                     /gene="recO"
                     /locus_tag="PKN85_08100"
     CDS             complement(360..1097)
                     /gene="recO"
                     /locus_tag="PKN85_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNT44409.1"
                     /translation="MSLHKDEAIVLSKRAYGESDRIIHLFTREAGKVSAFAKGAGKSQ
                     KRFMNTLEPFNHIRLEYFEKQGRGMVRIENADLVGANSGIEVSMRRACSASFFTEFVD
                     RLTREREPHSALFAALKEFIGLARTRDPVYADILHCELLMLDILGYHPNFDTCVHCGA
                     AMEESERLHFSRERGGILCAVCARSLPHRRCTQGLIPAFLSARAMQGGFATRALEREA
                     QDLMESFLAYHIDVDFRSYRLLKTLVF"
     gene            complement(1212..1736)
                     /gene="mobB"
                     /locus_tag="PKN85_08105"
     CDS             complement(1212..1736)
                     /gene="mobB"
                     /locus_tag="PKN85_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNT44410.1"
                     /translation="MSARSQKVFVLGVAGRSGSGKTTLIERLLPLLKGQGLRVAVIKH
                     THHDLEFDVPGKDSYRFKQAGAVTSVLVSPTGLGIVKDFREEPSLDEIISRYATDCDM
                     IIVEGFKHERAPKIEVFSADRGEPPLCLSGDETYIAIVTDEDIDTPLPRFTRDDITAI
                     ARFVTTRVLGRDIS"
     gene            complement(1733..2278)
                     /locus_tag="PKN85_08110"
     CDS             complement(1733..2278)
                     /locus_tag="PKN85_08110"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNT44411.1"
                     /translation="MIEEWELLESRVDRDYRVFRIQSELAMSPRTKKVGKFYTIDTRD
                     WVNVIPVTPEGQIVMIKQYRHGSKEVTLEIPGGLVDEEDPREAAQRELLEETGYAGDR
                     VELLGFVNPNPAIFNNRCHTYLIENAREISEKALDPDEDIEVVLMSADEIPSIIAGGG
                     INHSLVIVAFHFYFMKKLCSQ"
     gene            complement(2370..>2589)
                     /locus_tag="PKN85_08115"
     CDS             complement(2370..>2589)
                     /locus_tag="PKN85_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44412.1"
                     /translation="DIAACAAGISSPVSYVVTLIVNADGTVKDAKFKDGKNNSQEQCI
                     RSLMKQLKFTATRDGKEASVTVTILLAP"
BASE COUNT          495 a          748 c          755 g          591 t
ORIGIN      
        1 agcctaccaa gatccggcgg ccttctcgtc ccgccgtccc ggcgggagag aaaaacaata
       61 tccttattct gaagaaacta ttcccccctc atcctacctg tagtcatagg acctatgggt
      121 cctatatgtc ccataggtcc tatgaccatc ctccaacccc tacactacct caccccacaa
      181 ctcctatcct ctccccatca ttcccgtctg aagacaaaac tctctttttg cctcccgtcg
      241 cagacgggat gcaaagccgg ccggattttg ggaggcttcg aaatccggcc gaatcttttc
      301 tctgcgatct ctgcgggctt gagtcccgcc agggcgggac gggcgagagg cacggtcttt
      361 tagaaaacca gtgttttcag cagccggtag gatctgaagt ctacgtctat gtggtaggcg
      421 aggaagcttt ccatcaggtc ctgggcctcg cgttcgaggg cgcgcgtggc gaagccgccc
      481 tgcatagccc ttgcggagag gaaagcgggg atgaggccct gcgtgcatcg tctgtgcggg
      541 agggaccttg cgcagacggc gcagaggatg ccgccgcgct cgcgggagaa gtggagcctt
      601 tcggattcct ccatggcggc gccgcagtgg acgcaggtgt cgaagttggg gtggtagccg
      661 aggatatcaa gcatgagaag ttcgcaatga aggatgtcgg cgtagacggg atcccgtgta
      721 cgggcgaggc cgatgaactc cttcagggcg gcgaagagtg cgctgtgggg ctccctctcc
      781 cgcgtcagcc tgtcgacgaa ttcggtgaag aatgacgcgc tgcaggccct tctcatgctc
      841 acctcgatgc cgctgttggc tccgacgaga tcggcgttct ctatccgcac catcccccgg
      901 ccctgttttt cgaaatactc gaggcggatg tgattgaagg gctccagggt gttcatgaac
      961 ctcttctgac tcttgccggc acctttcgcg aaggccgaca cttttcccgc ctcacgggtg
     1021 aagagatgga tgatcctgtc cgattcaccg taggcgcgct tggaaaggac tatcgcctca
     1081 tctttgtgaa gggacatcgc acctccacaa acaggctgca accgtgaaga cggttgcagc
     1141 cgcttgaacc gttggggccg ttggggccgt tggggctgtt tgaacccttg aacccttgaa
     1201 cccttgaact ttcatgagat gtctcttccc agaacgcgcg tcgtcacgaa tcgggctatt
     1261 gccgtgatgt cgtcgcgggt gaagcgagga aggggagtgt ctatgtcttc gtccgtgacg
     1321 atggcgatgt acgtttcgtc accggagagg catagcgggg gttcgccgcg gtcggcgctg
     1381 aagacctcta tcttcggcgc cctttcatgc ttgaaccctt ccacgatgat catgtcacag
     1441 tccgtggcat agcgggagat gatctcgtca agggagggtt cttcccggaa gtctttcacg
     1501 atccccagtc ccgttgggga gacgagaacg gacgtgaccg ctcccgcctg cttgaaacgg
     1561 taggaatcct ttccgggaac gtcgaactcg aggtcgtgat gggtgtgctt gatgacggcg
     1621 acgcgaagac cttgaccctt gagcagggga aggagccttt cgataagggt ggtcttgccg
     1681 ctgcccgacc tgcccgcgac gcccagcacg aagacctttt gcgacctcgc actcattggg
     1741 aacagagttt cttcatgaaa tagaaatgaa aggcgactat cacgagggag tggtttatgc
     1801 cgccgcccgc tatgatggac ggtatctcgt cggcggacat gagcaccacc tcgatatctt
     1861 catcggggtc aagggccttc tccgatattt cccgcgcgtt ctctataagg tacgtgtggc
     1921 agcggttgtt gaatatggcg gggttcgggt tcacgaaccc gaggagttcg accctgtcgc
     1981 cggcatatcc cgtctcctcg agcaattccc gctgtgccgc ttcccggggg tcttcctcat
     2041 ccacgagccc gcccggtatc tcgagcgtta cctctttgga tccgtgccgg tactgcttga
     2101 tcatcactat ctggccctcg ggtgtaacag ggatgacgtt gacccagtcg cgggtgtcga
     2161 tggtgtagaa cttgcccacc ttcttcgttc tcggagacat ggcaagctcc gactgtatgc
     2221 ggaacacgcg atagtccctg tccaccctgg attcaagaag ttcccattcc tcgatcataa
     2281 cggataacaa aataccacgc ggcgggaggt aaatgcaaca tgcaaggccg gatctgacga
     2341 cccggccttg catgcagtgt ctgtttggct cagggcgcga gaagtatcgt gactgtcacc
     2401 gatgcctcct tgccgtcccg ggtcgccgtg aatttgagct gtttcatgag tgaccttatg
     2461 cactgctcct gggaattgtt cttcccatcc ttgaacttcg catccttcac cgtgccgtca
     2521 gcattcacta tcagggtgac aacatagctg acggggctgc ttatgccggc ggcacatgcc
     2581 gcaatgtcg
//
LOCUS       DAOQTR010000331         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_48140_length_2585_cov_3.315810, whole genome shotgun sequence.
ACCESSION   DAOQTR010000331 DAOQTR010000000
VERSION     DAOQTR010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48140_length_2585_cov_3.315810"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKN85_08120"
     CDS             <1..385
                     /locus_tag="PKN85_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT44413.1"
                     /translation="RFTSAHDCGRAINPMAVEGQMEGSIQQAGVAAITEGNLWDKGYL
                     LNPNLLEYKVPLACDMPDMDTIIVSSRDPAGPFGAKEGGLTVRMNAYSAVACAVANAT
                     GELFSELPLTPDRVMKMLERKKGVK"
     gene            385..1335
                     /locus_tag="PKN85_08125"
     CDS             385..1335
                     /locus_tag="PKN85_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNT44414.1"
                     /translation="MILPQFEYKRARSVADAVALYKKEKGKALYLAGGTDLIPLVKLR
                     LREPKAVIDLKGIEDLKIIATRGGSLSVGANATLFDLKKDPVIAAHFPALVESLDATA
                     CETLQMRGTLGGNILQGARCLEYNQSLEWRKARGFCFKMGGKECNVVKGAKVCFANYC
                     SDNAPSLISLGAQVRLMGPGGERTVKLESLFTGDARAPFAMEGGEVLTHILVPLKKTK
                     GAYEKLRVRESMDYPLVGAAVSVIDGKVRICVGGVGAAPRLYVLGDIRDAASVRDVAE
                     KASADAKPVANATLSPAYRKKMVGTLIKRALKRALAEGKG"
     gene            1332..1802
                     /locus_tag="PKN85_08130"
     CDS             1332..1802
                     /locus_tag="PKN85_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNT44415.1"
                     /translation="MKTMKVSFNVNSEDVTLDVRPYETLLETLRERLFLTGAKEACGL
                     GACGACTVIVNGIPLRSCLVLTPEVEGATIMTVEGLREDGNLHPLQETFMEKGAVQCG
                     FCTSGMIMTAKALLDRNKRPTKKEIVTTISSNVCRCTGYKKIVEAVEEAAKRMA"
     gene            1942..2235
                     /locus_tag="PKN85_08135"
     CDS             1942..2235
                     /locus_tag="PKN85_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44416.1"
                     /translation="MKIRVMVDSNLRIKNVFEPPIELEMSQDENTLKDVLGKLSERYP
                     YLRFLEKGEMGDDLHHLYLNGESHFSFPEGLNRKISEGDTVRVDAYMDPLAGG"
     gene            complement(2354..>2585)
                     /locus_tag="PKN85_08140"
     CDS             complement(2354..>2585)
                     /locus_tag="PKN85_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44417.1"
                     /translation="VLRPGGTAFVGGGFGKYTPPEVIRPLADRSRELNYLIGKKETTV
                     DEIQKDLEAAGISENAEFITEGGLWVIIRKDA"
BASE COUNT          570 a          700 c          810 g          505 t
ORIGIN      
        1 ccgtttcacc tccgcccacg actgcgggcg cgccataaat cccatggccg tcgagggcca
       61 gatggaaggc tccattcagc aggcgggggt tgcggccatc accgagggga acctgtggga
      121 caaggggtac ctcctgaacc ccaaccttct tgagtacaag gtccctctgg cgtgcgacat
      181 gcccgatatg gacaccatca tagtgagctc gcgggaccct gccggacctt tcggcgccaa
      241 ggaaggaggc ctgacggtgc gcatgaatgc gtacagcgcc gttgcctgtg ccgtcgcgaa
      301 cgccacggga gagctgttca gcgaactgcc tctgacgccg gacagggtca tgaagatgct
      361 ggaacgaaag aagggggtga agtgatgatc ctgccacagt ttgaatacaa gagagcacgg
      421 agcgtggccg acgcggttgc cctctataag aaagagaaag gaaaggcgct ctacctcgcc
      481 gggggaacgg acctgatccc gctcgtgaag ctgaggctgc gggagccgaa ggcggtcatc
      541 gacctgaaag ggatagagga cttgaagatc atagccaccc gcggcgggtc cctttcggtg
      601 ggcgcgaatg ccaccctctt cgatctcaag aaggaccccg tgatcgccgc tcacttcccg
      661 gctctcgtcg agtcactcga tgccacggcc tgcgagacac tccagatgcg cggcactctg
      721 ggcggcaata tccttcaggg tgcgcgctgc ctcgagtaca accagtccct cgaatggcgg
      781 aaggcccggg gtttttgttt caagatgggc gggaaggaat gcaacgtcgt caagggcgcg
      841 aaggtatgct tcgccaatta ttgcagcgac aatgccccct ccctcatcag tctcggggca
      901 caggtgaggc tcatgggacc tggcggggag cgcaccgtga aactggaaag ccttttcacc
      961 ggggatgcca gggcgccctt cgccatggag ggaggagagg tgctgacgca catactcgtt
     1021 cccctcaaga agacaaaggg ggcctacgag aagctgcgcg tccgtgagtc catggactac
     1081 cctctcgtcg gcgccgccgt gtctgttatc gacgggaagg tgaggatctg cgtgggcggc
     1141 gtcggcgctg ccccccgcct gtatgtcctc ggggatatca gggatgccgc ctctgtcagg
     1201 gacgttgccg aaaaagcctc cgctgacgca aaaccggtgg cgaacgccac gctctcaccg
     1261 gcctaccgga aaaagatggt ggggaccctg atcaagcggg ctctcaagag agctcttgcg
     1321 gagggtaaag gatgaaaacg atgaaagtca gcttcaatgt aaacagtgaa gatgtcaccc
     1381 tcgacgtgag accctacgag acccttctcg aaacccttcg tgagcggctg ttcctcacgg
     1441 gagcgaagga agcctgcgga ctgggtgcat gcggggcctg cacggtgata gtgaacggga
     1501 taccgctgag gtcctgtctg gtcctgaccc ccgaggtgga gggagcgacg atcatgacgg
     1561 tggagggctt gagagaggac gggaacctgc accccctgca ggagacgttc atggagaagg
     1621 gcgccgtcca gtgcggtttc tgcacctcgg gcatgatcat gaccgcgaag gccctcctcg
     1681 acaggaacaa gaggccgaca aagaaggaga tcgtcacaac gatatcctcc aacgtctgcc
     1741 ggtgcacggg ctacaagaag atagtcgagg cggtggagga agcggccaaa aggatggctt
     1801 gaacggttta aaccgcttga accgcttgag cgtcaaaagc catcgcaggt cctgtagggc
     1861 ggatatgtcc gatgggacac gtgttttttt cctcgtaatg ttgtatatta ttaagcagat
     1921 tggcggtatg gaggtgggac gatgaagatc agggtcatgg ttgattcgaa ccttcggatc
     1981 aagaacgttt ttgagcctcc gatcgaactg gagatgagtc aggacgagaa tacactgaaa
     2041 gatgttctcg gcaaactctc cgagcggtat ccctacctga ggttcctgga gaagggagag
     2101 atgggcgatg acctccatca cctgtatctt aacggagaaa gccatttttc attccccgag
     2161 ggactgaaca gaaagatcag cgaaggcgac acggtccgcg tggacgccta catggacccc
     2221 ctggcagggg gatgaaaaga cggcttgaat agcttgagtc gcttgaatgg cttgagggtt
     2281 acaggcaatc cgagcatcat tggttacaac cgtttccaaa gaggttcttt tagtctttca
     2341 actctcaagc gattcaagcg tcttttctga ttattaccca caacccccct tcagtgatga
     2401 actcggcgtt ttcgcttatg cctgccgctt caaggtcctt ctgtatttcg tcgacggtgg
     2461 tctctttctt gcctatgagg taattcagtt cccgtgagcg gtccgcgagg gggcggataa
     2521 cctccggggg tgtgtatttg ccgaagcctc cgcccacgaa ggccgtcccg ccgggtctga
     2581 ggacg
//
LOCUS       DAOQTR010000332         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_48475_length_2574_cov_4.142120, whole genome shotgun sequence.
ACCESSION   DAOQTR010000332 DAOQTR010000000
VERSION     DAOQTR010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48475_length_2574_cov_4.142120"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKN85_08145"
     CDS             <1..425
                     /locus_tag="PKN85_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT44418.1"
                     /translation="EGHAMTDTESKAIQAKSKTEAATPAEQTRPGLVFTPAVDIFETD
                     KEITLLADMPGVRGEDLLIDLHENVLTLDGNVRPPEGPGEVDVFREYRTGKYYRQFTL
                     SEIIDQAEIKARLEGGVLRLTLPKVKAATPRRIMVKGQ"
     gene            complement(604..2007)
                     /gene="hydG"
                     /locus_tag="PKN85_08150"
     CDS             complement(604..2007)
                     /gene="hydG"
                     /locus_tag="PKN85_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNT44419.1"
                     /translation="MDRHEKEKPFIDRDKLYNLIEEKAPSENELNAILNKALQFKGLD
                     QREVASLLRVSDPGQIHRIMEAARTVKEHIYGRRLVFFAPVYTGNVCVNNCLYCAFRS
                     DNKSLKRTVLTMPEIEAEVASLLKDGHKRILLICGESERNGTDYMCEAIRTTYAVREG
                     GNYVRRINIEIAPLPVEDFSRLKKEKIGTYVCFQETYDPVLYREYHPAGTPKADYDYR
                     LTVFDRAMQGGIDDVGLGVLFGLADYRFEILALMEHAHHLEAVFGCGPHTISFPRIEP
                     ADNAPLTKDIPYPVSDDDFKKIIAIIRISLPYTGIILSTRETEEMRTELFDHGVSQIS
                     AGSRTNPGAYSKGQDHDYGSQFSLGDHRSLEEVISSIIEKEYIPSFCTGCYRKGRVGN
                     DFMDLAKPGLIKEFCMPNAVFTFKEYLLDFASPETKERGLRLIDKLVSDVKKKSLKTR
                     MTAVLGQIDHGARDLYF"
     gene            complement(2056..2289)
                     /locus_tag="PKN85_08155"
     CDS             complement(2056..2289)
                     /locus_tag="PKN85_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44420.1"
                     /translation="MLILLTSKTSVPRVNTLLSEYAEIILGRQGIPIRDHGINIISLV
                     VEGNTDQINALTGKIGKLEGVEVRSILTKYREQ"
BASE COUNT          502 a          722 c          739 g          611 t
ORIGIN      
        1 aggagggaca tgcaatgacg gatacggaaa gcaaggccat tcaggctaaa agtaaaacgg
       61 aagcggccac accggccgag cagacgaggc cgggcctcgt gttcacgccg gcggtcgata
      121 tctttgaaac ggataaagag atcaccctgc tcgcggacat gcccggtgtg cggggtgaag
      181 acctcctcat tgaccttcac gagaatgttc tcaccctcga tgggaacgta aggcctcccg
      241 aaggtcccgg tgaggtggac gtgttcaggg aatacagaac gggaaagtat tacaggcagt
      301 tcacgctgtc agagatcata gatcaggccg aaataaaggc acggcttgag ggcggcgtcc
      361 tcaggttgac actgccgaag gtaaaggccg ccacaccgcg gaggataatg gtcaagggcc
      421 aatagggacc ttgccggagg cgcccttcac gtttcccgtt ccgggcggca ggggcggaag
      481 aggccgtcat tctctgcacg gaaggtctct cacgcctgtg aggtgttcat gcctgggtgg
      541 cagaatacct ggtccggaat gtgaggccgt gcggggcgtc tccggactcc tcgagtctgt
      601 ccgctaaaaa tagaggtcgc gggccccgtg gtcgatctgg cccaggaccg cggtcatcct
      661 tgtcttgagg gactttttct tcacgtccga tacaagcttg tcgatgaggc gcagccccct
      721 ctcctttgtc tcgggagacg cgaagtcgag caggtactcc ttgaaggtga acaccgcgtt
      781 gggcatgcag aattccttta tgagacccgg cttggctagg tccatgaagt cgttcccaac
      841 ccttcctttc ctgtagcagc cggtacagaa cgaggggata tattccttct cgatgatcga
      901 cgatatgacc tcttcgaggc tccggtggtc cccgagagag aactgacttc catagtcatg
      961 gtcttgcccc ttcgaatagg cccccgggtt cgtgcgggag ccggccgata tctgactgac
     1021 gccgtggtcg aacagctcgg tcctcatctc ctcggtctct ctcgtgctca gaatgatgcc
     1081 tgtgtagggg agggagatcc tgatgatcgc gatgatcttc ttgaaatcgt catcgctgac
     1141 ggggtaaggg atgtccttcg tgagcggcgc gttgtccgcg ggttcgatcc tgggaaagct
     1201 tatcgtgtgc gggccgcagc cgaaaacggc ctcgagatga tgcgcgtgct ccatgagggc
     1261 gaggatctca aagcgataat cggcaagacc gaaaagcacg cccagcccca catcgtctat
     1321 tccaccctgc atggccctgt cgaagaccgt gagcctgtaa tcgtaatccg cttttggcgt
     1381 cccggcggga tggtactccc tgtacagcac cggatcgtag gtttcctgaa aacagacgta
     1441 ggtgccgatc ttctccttct tcaacctgct gaagtcttcc acgggaagag gggcgatctc
     1501 gatgtttatc cggcgcacgt agtttccccc ctcgcggacg gcatacgtcg tgcggatggc
     1561 ctcgcacatg tagtccgtgc cgttcctctc cgattcaccg cagatgagga ggatcctctt
     1621 gtggccgtcc ttaaggagcg acgccacctc ggcctcgatc tcaggcatcg tgaggacggt
     1681 gcgcttcagg gacttgttgt cactgcgaaa ggcgcagtag aggcagttgt tgacgcagac
     1741 attcccggtg tagacgggcg cgaagaatac gaggcgccgc ccgtagatgt gctccttgac
     1801 ggtccgcgcc gcctccatta tccggtgtat ctgtccgggg tcgctcaccc tgagaagtga
     1861 ggcaacttcc cgctggtcaa ggcctttgaa ctgcaaggcc ttgttcaata tggcgttcag
     1921 ttcgttctct gacggagcct tttcctctat gaggttgtac agcttgtccc tgtcgatgaa
     1981 gggcttttct ttctcgtgac ggtccatgtc tattctcctt gtccgccgtc agacgcgaaa
     2041 cgccgcgctt gcgtctcact gctcacggta ttttgtgaga atagatctta cctccactcc
     2101 ctcgagctta ccgatcttac cggtaagggc gtttatctga tcagtgtttc cttccacgac
     2161 gagagagatg atgttgatgc cgtggtcgcg gatggggatc ccctgtcttc ccaggatgat
     2221 ctccgcgtac tccgagagca gggtgttgac cctgggcacg ctcgtcttgc tcgtcagaag
     2281 gatgagaatt gcaccaaatc tcttttctat cggaccttct cctctagatc agaatgaacg
     2341 ttgacacttc gaaagagata aaatctattt ctcagttctc ctaactataa ctcacgacga
     2401 gagagggtgc aaatgctttt tatcatcgcc gctcctctcc ccaagtgttt tccttaccgc
     2461 ggttccttcg caaccggttt tttcgtgttc ccctgcaatg ttcttccccg cttgttctcc
     2521 ggggcttctt tctggctgat acggaaggcc ttgccggcaa cggtgatggt gccc
//
LOCUS       DAOQTR010000333         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_48508_length_2573_cov_3.681096, whole genome shotgun sequence.
ACCESSION   DAOQTR010000333 DAOQTR010000000
VERSION     DAOQTR010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48508_length_2573_cov_3.681096"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PKN85_08160"
     CDS             complement(<1..891)
                     /locus_tag="PKN85_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44421.1"
                     /translation="MKIGIVGAGLVGSTTAYTIAMKGFGTEIVMIDKDVSRAGAEAAD
                     ILHAVPFGHSAVIGQGDYPDLSGSSAVIVAAGRRRQPGQSRLDLAVDNAAMLRDIIPA
                     ILKHAPGAVLLITTNPVDVMTHEASRIAATMGVSPARVIGSGTVLDTARFRTLLGQFL
                     GIDSSHVHAYVVGEHGDSEVMAWSLARVGGLTMDEICRLLGRELGDGEKQKMGNDVRG
                     AGHRIVSGKGATYYGIGSALAHMVDVILHDHRAILTVSTFLSDVEGISDVTLSLPHIL
                     GGQGILARLPDVLRQSQALFD"
     gene            complement(930..2084)
                     /locus_tag="PKN85_08165"
     CDS             complement(930..2084)
                     /locus_tag="PKN85_08165"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44422.1"
                     /translation="MDTLSREQLYVLIEQRGSACVSLYMPSYRVDPEKRQNQIRFKNL
                     VRKARQALMAKGLRAREADGLLSVVTPLVDDDPFWSFVDSEGFCAFMSEKHLFRFFLP
                     VRFEERVAVMGRFYVKPLLGLVYNDSRFFMLTLGLSGARLFQCSRYGMNELAMEKVPG
                     SFEEAMQYSEQQKGLQLHAHTLAAGERRAAMFHGHGGGADGKKVEILEYFKVVDRGVT
                     GMIGHESAPLLFVGLDHFFGLYKKANSYPFLSGENVAVNPEDLTEAELHDKAVRMMDA
                     HFLGNRNQAVESYLDLCGTGRTAGDIETILPEAYGGRVDSLLLSTEAAKWGTFDPVTL
                     KVDVHEGPAGDDEDLFDLACVYTYLKGGAVYDIAAGTIPGIPPAAAIFRY"
BASE COUNT          522 a          809 c          734 g          508 t
ORIGIN      
        1 atcgaacagg gcctggctct gccgcagcac gtcgggaaga cgcgccagga tgccctgccc
       61 cccgaggata tggggcagcg agagggtcac gtcggaaatc ccctcgacat cggacaggaa
      121 ggtgctgacc gtgagtatcg ccctgtgatc gtggaggatc acgtcgacca tgtgggccag
      181 cgcgctgcct attccgtaat aggtggcccc ctttcccgag acgatccgat gtccggcccc
      241 gcgcacgtca tttcccattt tctgtttttc cccgtcgccc agttcccgtc ccagaagacg
      301 gcagatctcg tccatcgtta atccacccac cctcgcaagg gaccaggcca tgacttccga
      361 atcaccgtgc tctcccacga cataggcatg gacatgactg ctgtcgatac caaggaactg
      421 tcccagcaac gtccggaaac gggcggtgtc gaggaccgtg cccgacccga tcaccctggc
      481 cggggacaca cccatggtag ccgcgatgcg gcttgcctcg tgggtcatca cgtccacggg
      541 gttggtcgtt atgaggagca cggcgccggg cgcgtgcttg aggatggccg ggatgatatc
      601 cctcagcatg gcagcattgt cgaccgcgag atcgagccgg gactggccgg gctgcctcct
      661 cctccccgcg gccacaatga ccgcggagct ccccgaaagg tcaggatagt cgccctgtcc
      721 tatgacagcg gaatggccga agggaacggc atgaagaata tcggccgcct ccgcgccggc
      781 acgcgatacg tccttgtcga tcatgacgat ctccgtacca aatcctttca tagcgatggt
      841 gtaggcggtt gtggacccca ccagaccggc gccgacgatc cctatcttca tggctttcat
      901 ctccttcgcc tttcccctgg cccccgcgat cagtaccgga agatggcggc ggccggcggg
      961 atacccggga tcgttccggc cgcgatgtcg tagaccgcgc ctcccttcag gtatgtgtac
     1021 acacaggcga ggtcgaacag gtcctcatcg tcgcctgccg gtccctcgtg gacatcgacc
     1081 ttcagggtga cagggtcgaa ggttccccat ttcgccgctt cggtagagag aagaagggaa
     1141 tcgacgcgtc ctccgtatgc ctcaggcagg atggtctcga tgtctcccgc ggttcttccc
     1201 gttccacaaa ggtcaagata ggattcgaca gcctggttcc ggttgccgag gaaatgggca
     1261 tccatcatcc tcaccgcttt gtcatggagc tcggcctcgg tcaggtcttc aggattcacg
     1321 gccacgttct cgccggagag aaagggataa ctgttggcct ttttgtacag gccgaaaaag
     1381 tggtcgaggc cgacgaagag aagaggcgcg ctctcatggc ctatcatgcc cgtcacgccg
     1441 cggtcgacca ccttgaaata ctccaggatc tccaccttct tgccgtccgc cccgccgccg
     1501 tgcccgtgga acattgccgc ccgcctctcg cccgctgcaa gggtgtgcgc gtgcaattga
     1561 agacccttct gctgctcgct gtactgcatg gcctcctcga aactcccggg gaccttctcc
     1621 atggcgagtt cgttcatgcc atagcgggag cactggaaga gcctcgcacc actgaggcca
     1681 agagtaagca tgaaaaaccg gctgtcgttg tacacaagtc ccaggagggg cttgacatag
     1741 aaccttccca tcacagcgac cctttcctcg aaccggacgg ggaggaagaa acggaagaga
     1801 tgcttctccg acataaaagc gcagaaacct tcgctgtcaa caaaggacca gaaggggtca
     1861 tcgtcgacaa gaggcgtcac aacagacagc aggccatccg cctcccgggc ccgcagaccc
     1921 tttgccatca atgcctgcct ggccttcctc acaaggttct tgaaccgtat ctggttctgc
     1981 ctcttctcgg gatccacccg gtaggaaggc atgtacagcg aaacgcacgc gcttcccctt
     2041 tgctcgatca ggacgtacag ctgttctctt gaaagagtgt ccattttcac ccacctttgg
     2101 attatcgctg gaccccgggg cgggaacggc agaacccccg tcttcaacac atctgttgag
     2161 gtccatgacc cgtgggtgat gcaagggagg tgccaacctc ccgaaagcct gtggaacctt
     2221 gcaatccgag acggtcacct atggagggag cggagataat ggggaataat gccacaagga
     2281 aacgtcgcag ttcgcaggtc gcagagtctg gcggggggag ggaacacatc tccgtttcgc
     2341 acttgcgtcc ggtcattttt tgacctatcc ttacttcatg ctctcccctg tgaggttctc
     2401 catcatgacc tacaatgttc acagctgcat cggaaacgac ggaaaggaca tgcccggaag
     2461 gatcgctcgt atcatcgcgg cgctcaatcc cgatatcgtg gcgctccagg aactcgatgt
     2521 gggtctcaac agaacgggcg gcgtggacca ggcgcacctg atcgcatgtg aac
//
LOCUS       DAOQTR010000334         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_49415_length_2543_cov_4.248794, whole genome shotgun sequence.
ACCESSION   DAOQTR010000334 DAOQTR010000000
VERSION     DAOQTR010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49415_length_2543_cov_4.248794"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1396)
                     /locus_tag="PKN85_08170"
     CDS             complement(194..1396)
                     /locus_tag="PKN85_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNT44423.1"
                     /translation="MLKTLNIPDLTPKEEITARIDKLRKGMVEKGIELAVILQNVDIF
                     YFTGTLQRGVLVVSAEQGPQFFVEKSVYRAGMETPLDIIPIKRDKEVKDLLAEKKMLH
                     GRCAMEFDVVPVAICERWKSLLGKDRMEDLSPIIRDGRLVKSEFEVAQFRKSGEIVER
                     VFEKAKDIIKEGIREIDIEAELVAEGRKWGHQGFLRMRGLNQEMMNLYVTHGYSTTIT
                     SGADVPISGIGVTHAIAQGASVNTVKRGIPVIVDYGGGYNGYITDETRAYAVGPLKDL
                     FQKPFEVARDIINETMDTAKEGTDATEVFGRAMDRVKTAGLEEHFMGHGEGQVSFIGH
                     GLGLEINELPVITPRHRMILKEGMVFAFEPKFIFPGEGAVGIEVDFVVRKNGLERLTK
                     TPLDIVYV"
     gene            complement(1371..>2543)
                     /gene="proS"
                     /locus_tag="PKN85_08175"
     CDS             complement(1371..>2543)
                     /gene="proS"
                     /locus_tag="PKN85_08175"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNT44424.1"
                     /translation="YVSIFTRCGFRFRVVEADTGQIGGSFSHEFMVLADTGEDIIISC
                     DACGYAANLERAEVGFTDGPVSGKKGLHRRVETPNQRKIDEVSSFLGITPDKLLKTII
                     YNTDKGTIGVLVRGDREINDTKLKNLLKLEEMELADERTIEEATGGPLGFSGPIGLSI
                     PLYADMDLTFMEDFVIGGNERDVHVVDVNVGDLTVKGFYDIKVAVAGDRCPRCESGKL
                     VATRGIEVGHIFKLGLKYSKAMNATFLDSEGKDQFMVMGCYGIGVGRTVAAAIEQGND
                     ENGMILPLPIAPFEVDVLPVNTTHEESMKVARQIYEELLANGVDAILDDRDERPGVKF
                     KDCDLIGIPLRITIGERGLKEGIVEIKRRDSKEFEKVPAKEAARRVMNYVKNAKHP"
BASE COUNT          439 a          806 c          698 g          600 t
ORIGIN      
        1 aaacccttcc acctgggccg gatagcgctc cagcggttca aggctctcat gggcgtgacg
       61 gaaaggcagg agtgcaagcc cctgtcgtga atgcttcccc cggtgatgaa acatgcggtc
      121 acaggtcaca tgttgcaggt cacaggtcag aataacaaaa agatattcag tctggaacct
      181 ggaacgtgaa accctagacg tacactatgt ccaacggtgt cttcgtcagc ctttccaggc
      241 cattctttct gacgacgaag tccacctcta tgcctacggc gccttcaccg gggaagatga
      301 acttcggctc gaaggcgaag accatgccct ctttgagtat catcctgtgc cgcggcgtga
      361 tgacgggaag ttcgttgatc tcgaggccga ggccgtggcc gatgaagctt acctggcctt
      421 ccccatggcc catgaaatgt tcttccagtc ccgcggtctt gacccggtcc atggcccttc
      481 cgaagacctc cgtcgcgtcg gtcccttcct tcgcggtgtc cattgtctcg ttgatgatgt
      541 cccgggccac ctcgaagggt ttttgaaaga ggtctttcaa gggccccacc gcgtacgccc
      601 gcgtctcatc ggtgatgtac ccgttgtacc cgccaccgta atcgacgatt accgggatgc
      661 cccgtttcac agtgttgacg gaggccccct gagcgatggc gtgcgtcacg cctatcccgg
      721 atatgggtac atcggccccg gaggtgatcg tcgtggagta gccgtgggtg acgtagaggt
      781 tcatcatctc ctggttgaga ccgcgcatcc tgaggaatcc ctggtgtccc catttcctcc
      841 cttccgcaac gagttcggcc tcaatatcga tctcccggat cccttccttt atgatgtcct
      901 tcgccttttc aaagacgcgc tccacgatct cgccggattt tcggaactgg gcgacctcga
      961 attcgctctt tacgagcctc ccgtccctga tgatcggcga caggtcttcc atcctgtcct
     1021 tccccaggag cgatttccat cgttcacaga tcgcgacggg cacgacgtcg aactccatgg
     1081 cgcaccgccc gtgaagcatt ttcttctccg ccaggaggtc cttgacctct ttgtcccgct
     1141 tgatggggat gatatcgagg ggtgtctcca tcccggcgcg ataaacgctt ttttccacga
     1201 aaaactgcgg tccctgctcc gccgacacca cgaggacccc gcgctggagc gttcccgtga
     1261 agtagaagat gtccacgttc tgcaggatga cggcgagttc gatgcccttc tccaccatcc
     1321 ccttgcggag cttgtcgatc cgtgccgtta tctcttcctt tggtgtgaga tcagggatgt
     1381 ttagcgtttt taacatagtt catgaccctc cgtgccgctt cctttgcagg caccttttcg
     1441 aattccttgc tgtcccgtcg ttttatctcc acgatgcctt ccttgaggcc ccgttcccct
     1501 atcgtgatcc gaaggggtat cccgatgagg tcgcaatcct tgaacttgac acccggtcgc
     1561 tcgtcgcggt cgtcaaggat ggcgtcgacg ccgttcgcca ggagctcctc gtatatctga
     1621 cgggcgacct tcatgctctc ctcgtgggtg gtgttcacgg gcagcacatc cacctcgaag
     1681 ggggcgatcg gcaggggcag tatcatgccg ttctcgtcgt tgccctgttc gatggcggcg
     1741 gccacggtcc tgccgacgcc gatcccgtag catcccatga ccatgaactg gtccttgccc
     1801 tcgctgtcga ggaaggtggc gttcatcgcc ttgctgtact tgaggccgag tttgaagatg
     1861 tgccccacct cgatgccccg cgtcgcgacg agtttgccgc tctcgcaccg gggacagcgg
     1921 tcgccggcca cggccacctt tatgtcgtag aaccctttga ccgtgagatc acccacgttg
     1981 acgtccacca cgtggacatc acgttcgttg ccgcctatca cgaagtcctc catgaacgtg
     2041 aggtccatgt cggcgtaaag gggaatggaa aggccgatgg ggcccgagaa gcctaaaggc
     2101 ccgcccgttg cctcctcgat ggtcctctcg tcggcaagct ccatttcctc cagcttgagg
     2161 aggttcttca gcttcgtgtc gtttatctcc ctgtcacccc ggacgaggac gccgatcgtc
     2221 cctttatccg tgttgtagat gatggtcttg aggagcttgt ccggcgttat cccgaggaag
     2281 gaggagacct cgtctatctt ccgctggtta ggcgtctcca cgcgcctgtg gaggcccttc
     2341 ttcccggaga cgggcccgtc cgtgaacccc acctcggccc tttcgaggtt tgccgcatac
     2401 ccgcaggcgt cacaggagat gatgatgtcc tcgcccgtgt cggcgagcac catgaactcg
     2461 tgggagaagc tcccccctat ctggccggta tcggcctcga cgacgcggaa cctgaacccg
     2521 caccgcgtga agatgctgac gta
//
LOCUS       DAOQTR010000335         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_49426_length_2543_cov_2.916801, whole genome shotgun sequence.
ACCESSION   DAOQTR010000335 DAOQTR010000000
VERSION     DAOQTR010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49426_length_2543_cov_2.916801"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKN85_08180"
     CDS             <1..461
                     /locus_tag="PKN85_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44425.1"
                     /translation="AGRVGAVNVSEESGADECGRAEGVLGPVPEGLPEGALEGLEGTI
                     SAQPSPIFQGLSETIYYTVTQGDGGDLTGLSVNVVIADGETGKARQSFSAPRAHFKGR
                     SFAGSFTFSTGALEPGEYRVKLMIPPGSGSEAREIATTSFVIRRIDMVVT"
     gene            515..1321
                     /locus_tag="PKN85_08185"
     CDS             515..1321
                     /locus_tag="PKN85_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNT44426.1"
                     /translation="MPNDFIGDLSKTRLLDLISPLLSRKKSGMIQIKGGEVGEIHIEG
                     SNIVHARTGDLAGEEAVLAMMEWNAGRATFDWEAATEEQTVFMPTEQLLMIWTNREAE
                     WGRIRELIPSPNVVFRIPVNGCPDNRNVQANQWKVLALCNGMRSVSDIAETLGWQLFE
                     TSQTLCGMVKDGLLEKAAEKRVEQADRARRTVNGNFFPHIENELRKAMGPIAPIVIDD
                     KLAEFGESKEAFPEDRLSPFIQAIGEEISDASKRVLFTRLMTEYIARKQK"
     gene            1353..2303
                     /locus_tag="PKN85_08190"
     CDS             1353..2303
                     /locus_tag="PKN85_08190"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNT44427.1"
                     /translation="MATENKVTSGLLFRLIAGIFVPILLAFAFLGCVLFLNIDLGGFK
                     FLSIRGIWSQSMNELGAASLKESTASLNRLGEQIVQQKAEDVAREIEIALGSAKKRPA
                     METLRNDGTLKQIAVQKVGVTGYTAVHDAQAVNHFHSNPKLVGSDLHDLSGKLPQFWK
                     LLEKGLAGPVGGYYNWRDADGSIRPKYMYIAPVKGAGLFVAATTYIDEFSKPAQAITA
                     KMGQMQKTYAEQYNRRFGLILIILAVVLLILLGVIYLYSFSVVHPIRHLSEVADKISM
                     GDLKATVDVKAKGEIAVLAESIERMQASVRAAIERLQKRR"
BASE COUNT          576 a          672 c          828 g          467 t
ORIGIN      
        1 gtgctggaag ggttggggct gtgaatgtgt ccgaagagag cggcgccgat gagtgcggcc
       61 gggcggaggg ggttcttggg cccgtgccgg aaggcctgcc tgagggagcg ctggagggtc
      121 tggagggaac gatcagcgcc cagccgagcc ccatattcca ggggctgtcg gagaccattt
      181 actacacggt gacgcagggt gacggtggcg acctgacggg actcagcgtg aacgtcgtga
      241 tcgcggacgg cgaaacggga aaggccagac agtccttctc cgccccccgg gcccacttca
      301 agggcaggtc ctttgccgga agcttcacct tttcgacggg cgcgcttgaa cccggggagt
      361 atagagtgaa gctgatgatc ccgccgggtt ccggaagcga agcgcgggag atagcaacca
      421 cgagttttgt gatccgcagg atagacatgg tagtgacctg acgcggggcg gcggcacgcc
      481 gtcagtggca gaacgagcat gaaaggaggt gcttatgccg aacgatttca ttggggatct
      541 ttcaaaaaca aggctcctcg atctgatatc accgcttttg agcagaaaga aatcgggcat
      601 gatccagatc aagggaggcg aggtcggtga gatacacata gaagggtcga acatcgttca
      661 tgccaggacg ggagacctcg cgggcgaaga ggcggtcctc gccatgatgg aatggaacgc
      721 ggggagggcc accttcgact gggaggcggc gacggaggag cagacggtct tcatgccgac
      781 ggagcagctc ctgatgatct ggacgaaccg cgaggcggaa tgggggagga tcagggagct
      841 tatcccgtct ccgaacgtcg tcttccgtat ccccgtcaac gggtgcccgg acaacaggaa
      901 cgttcaggcg aaccagtgga aggtgctggc cctctgcaac ggcatgaggt ccgtttcaga
      961 catcgccgag accctcgggt ggcagctctt cgagacatcg caaacgctgt gcggcatggt
     1021 taaggacggc ctcctggaga aggcggcgga gaagagggtc gagcaggccg atagggcgcg
     1081 caggaccgtg aacggcaatt tctttcctca catcgagaat gagctgcgga aggccatggg
     1141 ccccatcgcc cccatcgtca tcgacgataa gctcgccgaa ttcggggagt cgaaagaggc
     1201 gtttcccgag gacaggctct cgcccttcat tcaggccata ggcgaggaga tatcggacgc
     1261 ctcgaagagg gtcctgttca cgcgcctcat gaccgagtat atcgcacgga agcaaaaata
     1321 actaaatcct acacttacag gagggggttt gcatggctac agagaacaag gtgacatcgg
     1381 gattactttt caggctcatc gcgggaatct tcgtccccat cctgctggct ttcgcgtttc
     1441 tggggtgcgt gttgttcctt aacatcgatc tcggaggctt caaattcctg agcatccgcg
     1501 gtatctggtc gcagagcatg aacgaactcg gcgcggcaag cctgaaggaa tcgacggcat
     1561 cgctcaacag gctcggcgag cagatcgtcc agcagaaggc cgaggatgtc gccagggaga
     1621 tcgagatcgc cctggggtcg gccaagaaaa ggccagccat ggagaccctg cgcaacgacg
     1681 ggaccctgaa gcagatcgcc gtccagaagg tcggtgtgac gggttatacg gcggtccatg
     1741 acgcccaggc cgtcaaccat ttccactcca atcccaagct cgtgggatcg gaccttcacg
     1801 acctttccgg gaagcttccg cagttctgga agctccttga aaagggactg gccggacctg
     1861 tcggagggta ttacaactgg agggatgccg acggcagcat ccgtccgaag tacatgtaca
     1921 tcgctcccgt gaagggagcg ggtctctttg tcgccgccac gacgtacatc gacgagttct
     1981 ccaagccagc ccaggccata acggcaaaga tgggccagat gcagaagacg tacgcggaac
     2041 aatacaacag gcgtttcggc cttatcctca tcatcctcgc cgtcgtgctt cttatcttgc
     2101 tcggcgtcat ttacctttat tctttctccg tggtccatcc gatccgtcat ctttcggagg
     2161 ttgccgacaa gataagcatg ggcgatctca aggcgaccgt ggatgtcaag gcgaagggtg
     2221 agatcgccgt tctggcagaa tccatcgaaa ggatgcaggc aagcgtgcgg gcggccatcg
     2281 agagactaca gaagagaaga taacagggaa cgaccacagg ttccggggga aggggcctgt
     2341 ggtccaacga aagaacagag gtcgatatga tatatccaca gggaaaaacg atccatcaga
     2401 acctttccgc ggagtacacg gatgttccgc aactcctgtc gaccctcacg ggcaatggtt
     2461 tctcgggagt tgttgagata gaggggggag gcacgaaagg tgcgttcttg atcgccaggg
     2521 gccagatggt agatgcggtg gtg
//
LOCUS       DAOQTR010000336         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_49616_length_2537_cov_3.533844, whole genome shotgun sequence.
ACCESSION   DAOQTR010000336 DAOQTR010000000
VERSION     DAOQTR010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49616_length_2537_cov_3.533844"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..1278
                     /gene="lsrF"
                     /locus_tag="PKN85_08195"
     CDS             397..1278
                     /gene="lsrF"
                     /locus_tag="PKN85_08195"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HNT44428.1"
                     /translation="MPDIDGIKEAKNFYTDRPQTCEGFFLKGSNSLDWGLKNRLARIF
                     NPVSGRTVMLAIDHGYFQGPTTGLERVDINILPLVPYADTLMLTRGILRSVVPPSTSK
                     AIVLRVSGGASILKELSNEDIAVDIEESIRLNVCAMAVQVFIGGEYEKESIINMTKMV
                     DIGNRYGIPTLAVTAVGKDMARDAKYFQLACRICAELGAHYIKTYYVEKDFDTVTASC
                     PVPIVMAGGKKIPELDALTMAYNAVQQGASGVDMGRNIFQSDAPVAMIQAVSAVVHQN
                     MTPADAFDLYRSLKGKG"
     gene            1348..2322
                     /locus_tag="PKN85_08200"
     CDS             1348..2322
                     /locus_tag="PKN85_08200"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNT44429.1"
                     /translation="MEVPTPVPGPREMVVKIHACGICGSDIVEWYRLPRAPLVQGHEI
                     GAEVVAVGSAVQRFKPSDRVFIPPKIPCGSCVYCVKGHFPQCAEIKERLPGAFAEYVL
                     VPEVFVEKGTYPLPDNISYEQSTFIEPLACAARAQRLAGVRKGDTVLVMGCGMSGLLH
                     VKLAAARDCRVVAVDVNPMKLDFAAKAGAVMVVDGSEDIAERLIAGNGRKADIVVLCS
                     SARQAVEAAWRCVDKGGTVVLFAVPGPEEQVVVPVNDFWMKEITILTSYYCGPPDIME
                     AMDLIGSGTVTVHDLITHRLPLDDIVEGFRLVSDGRESIKVIIEPHRK"
     gene            complement(2335..>2537)
                     /locus_tag="PKN85_08205"
     CDS             complement(2335..>2537)
                     /locus_tag="PKN85_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44430.1"
                     /translation="YSFLSRRDGGTRRPPDLGRLRDPAAIFSLFSVSLCDLCELERSG
                     REIRFLSLSPTLSPHDQRTEQA"
BASE COUNT          526 a          711 c          810 g          490 t
ORIGIN      
        1 ggagcgctcc atgtcgactt gcacgatatc ggcggccgaa aggcggctcg tcatgccccc
       61 ctttcgcgtg atgtacaccc gcgaaccttc ccggatgctg atgtttcccg catgagagct
      121 cacgagcccc cgaaggaaca tgtcgcgccc gacttcgatg atctgcatga ttttcgcatc
      181 ctctgccatg acaccatcct ttcggtaagt ttttaacaca gatgggcgag gccggggaag
      241 gcttatcttt ggagcgctga gggtgaggtg ggagcggggg gggaggcaag gtcttcccct
      301 tttcggcttg aacggcaggg ttcattgttt tatactctgc gcagtaaagc tgtttctggt
      361 aacgccggat atttcacgtg aaagaaggag ataatgatgc ctgatatcga tgggataaag
      421 gaagccaaga atttctatac ggacaggccg cagacctgtg aggggttctt tctgaagggg
      481 tcgaattcgc tggactgggg tctcaagaac aggctggcgc ggatattcaa tcccgtgtcg
      541 gggcggacgg tcatgctcgc catcgatcac gggtatttcc agggaccgac gacgggcctg
      601 gaaagggtgg atatcaatat ccttccgctg gtgccttatg cggacacact gatgctgacg
      661 cggggcatcc tgcgcagcgt cgttccacca tcgacctcga aggcgatagt cctcagggtg
      721 tccggagggg caagcatcct gaaggagctt tccaacgagg acattgccgt cgacatcgag
      781 gagtccatac ggctcaacgt gtgcgccatg gccgtgcagg tgtttatcgg cggagagtac
      841 gagaaggaat ccatcatcaa catgacgaag atggtcgaca tcgggaaccg ctacggcatt
      901 cccacccttg ccgtgaccgc ggtggggaag gacatggccc gcgacgcgaa gtacttccag
      961 cttgcgtgcc gcatctgcgc ggagctcggc gcgcattata tcaagaccta ctacgtggag
     1021 aaggacttcg acaccgtcac agcctcctgc ccggtgccca tcgtcatggc gggcggcaag
     1081 aagatacccg agctcgacgc cctgaccatg gcctacaacg cggtgcagca gggagcgtcc
     1141 ggggtggata tggggcgcaa catcttccag tccgacgcgc ccgttgccat gatacaggcg
     1201 gtgagcgccg tggtgcacca gaacatgacc ccggccgacg cgttcgacct gtacaggagt
     1261 ctcaagggaa agggttgacg gaagaccgat cgacgaggga tagatattaa agtctcttac
     1321 tggtacaaca acagggacat acgcatagtg gaggttccga cgcctgtccc cggcccgcgg
     1381 gagatggtgg tgaagatcca cgcctgcggt atctgcggga gcgacatcgt ggaatggtat
     1441 cgccttccgc gggcgccgct tgtccagggt cacgagatag gcgccgaggt tgtggcggtg
     1501 gggtcagccg tgcagaggtt caagccgtca gaccgggtct tcattcctcc caagataccc
     1561 tgcgggtcat gcgtctactg cgtgaaaggg cactttcccc aatgcgcgga gatcaaggag
     1621 cggttgcccg gagcctttgc cgaatatgtc ctcgtgccgg aggtgttcgt agagaaaggg
     1681 acgtaccctc tcccggacaa catcagctac gaacagagca ccttcataga gcccctcgca
     1741 tgcgccgccc gcgcccagcg tcttgcgggg gtgaggaaag gggatacagt cctcgtcatg
     1801 ggctgcggaa tgtcgggtct gctccacgtg aagctggcgg cggcgaggga ttgcagggtg
     1861 gtcgccgtcg acgtcaaccc gatgaagctc gactttgccg caaaggccgg cgcggtcatg
     1921 gttgtcgatg gttcggaaga catcgcggag agactgatcg ccgggaacgg caggaaggcg
     1981 gatatcgtgg tcctgtgctc ttcggcgcgt caggccgtgg aggccgcctg gcgatgcgtc
     2041 gacaagggcg gaacggtcgt cctcttcgct gtgcccggcc ccgaggaaca ggtcgtggtg
     2101 cccgtcaacg acttctggat gaaggagatc accatcctga cgtcctacta ctgcggtccc
     2161 cccgacatca tggaggcaat ggatctcatt ggatcgggca cggtgaccgt tcacgacctc
     2221 ataacccacc gccttcccct cgacgacata gtcgagggct tccgactcgt ctccgacggc
     2281 cgcgaatcga tcaaggtcat catcgagccg cacaggaaat agagaggcgt tatctcacgc
     2341 ctgttcggtg cgctgatcgt gaggcgagag cgtgggggag aggctcaaaa accttatctc
     2401 tcgcccgctt cgctcgagtt cgcagaggtc gcaaagagag acagaaaaga gagaaaagat
     2461 tgccgccgga tctcggagcc tcccaagatc cggcggcctt ctcgtcccgc cgtcccggcg
     2521 ggagagaaaa gaatatc
//
LOCUS       DAOQTR010000337         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_49882_length_2529_cov_2.991512, whole genome shotgun sequence.
ACCESSION   DAOQTR010000337 DAOQTR010000000
VERSION     DAOQTR010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49882_length_2529_cov_2.991512"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKN85_08210"
     CDS             complement(<1..330)
                     /locus_tag="PKN85_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44431.1"
                     /translation="MIRFDAHFFDGRTSRALPVTVDFDGVTLAFGNEMDGTAVRASII
                     DCSIDPAVGRTKRSIRLPGGPLLQTYDHNAVRTMERSLGVNRSSGLVHFLESRWKMVA
                     VCMVCLAL"
     gene            complement(327..1448)
                     /locus_tag="PKN85_08215"
     CDS             complement(327..1448)
                     /locus_tag="PKN85_08215"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HNT44432.1"
                     /translation="MSPGPGRDCPKDDAFARETDDNLAERRPEEVRYRFAFTAITGEY
                     FRIWIVNLFLTVITLGVYAAWAKVRSRRYIWANINLAGHPFTFHGRPAAILRGNMIIA
                     ALFIAYVSMKRFEPSFAGILFILFTFIFPFLVCKSLRFNAHNTSYRNIRFHFRGTLRE
                     SYETYLLFAGIVPFTLFLFFPAWQFYKKRFFLGNLAYGTTGSSFAGRKGPFYKAYFFA
                     VVLGIIVFIALSSLAAVGVHRLTTGSAVEWGPLKVIYSLFFTYLLGATILYTLQALVY
                     SMTMNHCWEKTRLGEVRFVSTLKARRLLFIRITNMAAILCSLGLLIPWAKIRRTRYIL
                     DNLAVVTSRGFDGFASRDASDTTAVGDAATDFVGIEIGL"
     gene            1680..2450
                     /locus_tag="PKN85_08220"
     CDS             1680..2450
                     /locus_tag="PKN85_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNT44433.1"
                     /translation="MVSRALWKGQIHFGDFDVPVKLHTAVREQRIQFHLLHSRDHVRL
                     RQHMLCAYEKVPVPREEQVRGFEVGEGRYIIVDDDELERLEPEGNRTIEIHDFVRGGQ
                     IDPLYFDRVYYLAPDVMLRGYRALAEALGETGMEGICTWTMRKRSYLGSLAAKGKAMR
                     LITLRHADEVIDAGSLDLPVTGLSDRELEAGVNLVTALSAPFQPEKFKNDHQKKLREL
                     IDRKAAGEKISILRPRQRRATPPDRLLETLEASLKKAV"
BASE COUNT          573 a          655 c          832 g          469 t
ORIGIN      
        1 gagggcgaga cataccatgc agaccgccac catcttccag cgggactcga ggaagtggac
       61 gagacctgag gagcggttca cgcccaggct gcgctccatc gtgcgcaccg cgttgtggtc
      121 gtatgtttga agcagggggc ctccgggcag ccgaatggac cgctttgtcc ggccgacggc
      181 agggtcgatg gaacagtcga tgatcgatgc tctcaccgcg gtgccgtcca tctcgttccc
      241 aaaggcaagg gtgactccgt cgaaatcgac ggtcacgggc agggcccggg atgtccttcc
      301 atcgaagaaa tgagcgtcga agcgtatcat aggcctatct ctataccgac gaaatccgta
      361 gcggcatcgc caacggccgt cgtatccgag gcatcgcggg aagcgaaacc gtcgaagccg
      421 cgggaggtga ccacggcgag gttgtcaagg atgtagcggg tgcggcgtat ctttgcccag
      481 gggatgagaa ggcccagcga gcagaggatg gccgccatgt tggttatcct tataaaaagg
      541 agtcttcgcg ccttgagggt gctcacgaac cggacctctc cgaggcgcgt cttctcccag
      601 cagtggttca tcgtcatgga gtagacgagc gcctgaaggg tgtagaggat ggtcgccccg
      661 aggaggtagg tgaagaagag ggaatagatg accttgagcg gtccccactc tacggcggaa
      721 cccgtggtaa ggcggtggac gccgacggcg gcaagggagg acagagcgat gaaaacgatg
      781 atccccagga ccacggcgaa gaagtaagcc ttgtagaagg gccctttgcg tccggcgaag
      841 gagcttcccg tcgtgccgta tgccagattg cccaggaaga accttttttt gtagaactgc
      901 caggccggga agaagaggaa gagggtaaag gggacgatgc cggcaaagag aagatacgtc
      961 tcgtagcttt ccctgagggt gccgcgaaag tgaaagcgga tgttcctgta agaggtgttg
     1021 tgagcgttga aacgcaggga cttgcagaca aggaagggga agatgaaggt aaagaggatg
     1081 aacagaatgc cggcgaagga gggctcgaac cgtttcatcg agacgtaggc gatgaagagc
     1141 gccgcgatga tcatgttccc cctcaggatc gccgccggtc tgccgtgaaa ggtgaagggg
     1201 tgacctgcca ggttgatatt ggcccatatg taccgtcggc tgcgcacctt ggcccaggcg
     1261 gcgtacaccc ccagggtgat gacggtgagg aagaggttta cgatccagat cctgaaatac
     1321 tcacccgtta tggcggtgaa ggcgaagcga taccgcactt cttcaggcct cctctcggcc
     1381 aggttgtcgt ccgtttcccg cgcgaaggcg tcgtctttcg gacaatcccg ccccgggccc
     1441 ggcgacaatg gggcctcggt caggcgatca ggttcattcg agatgcggct caccctcggg
     1501 tacgttcctc cggtattatc ggtccgttta ttataaacgt gggtgacgga attaaaaagg
     1561 attttctcgg tgcgccgaag atcggcccga taccgcgtcc cgagtgtccc ggcatgcgcc
     1621 ggagagtttg acagaaagac ctttcgtggg tatctgttct agataaggga ggtggaccca
     1681 tggtatcgcg ggccctctgg aaaggacaga tccatttcgg cgatttcgac gttccggtaa
     1741 agctgcacac cgcggtcagg gagcagcgga tacaatttca tctccttcac agtcgcgatc
     1801 atgtgcggct caggcagcac atgctgtgtg cctatgaaaa ggtgcccgtg ccccgggagg
     1861 agcaggtccg tggttttgaa gtgggtgagg ggaggtacat catcgttgac gacgatgaac
     1921 tggagcgcct tgagccggag ggcaacagga cgatagagat ccatgatttc gtgaggggcg
     1981 ggcagataga cccgctttac ttcgaccggg tctattatct ggcgcccgac gtgatgctgc
     2041 ggggataccg ggccctcgcc gaagcactcg gagaaacggg tatggagggt atatgtacct
     2101 ggaccatgag gaaacgatcc taccttggat ccctggcggc aaaggggaaa gccatgaggc
     2161 tgatcactct gcgccatgcg gatgaggtca tagacgcggg atctctcgat ctgcccgtga
     2221 ccggcttgtc cgacagggaa ctcgaggcgg gggtcaatct ggtcactgcc ctgtcagccc
     2281 cttttcagcc ggagaagttc aaaaatgacc atcagaagaa actccgggag ttgatcgaca
     2341 gaaaagccgc gggagagaag atatccatcc tgcgccccag acaacgcagg gccaccccac
     2401 cggacaggct cctggaaaca ctggaagcga gcctgaaaaa ggcggtgtga gaacgggtag
     2461 aagggaggcc gcatggcact gcaatccatc tggacgggaa cgatcacttt cagcctcgtg
     2521 gcaataccg
//
LOCUS       DAOQTR010000338         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_49951_length_2527_cov_3.350324, whole genome shotgun sequence.
ACCESSION   DAOQTR010000338 DAOQTR010000000
VERSION     DAOQTR010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49951_length_2527_cov_3.350324"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKN85_08225"
     CDS             complement(<1..232)
                     /locus_tag="PKN85_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44434.1"
                     /translation="MKINSQHIPALYLTAVLPITLLGLLLLGGCASVGPDYVRPDVSV
                     SDQWHTALKGGLKPAAADPGVLAAWWTTLNDEA"
     gene            complement(271..819)
                     /locus_tag="PKN85_08230"
     CDS             complement(271..819)
                     /locus_tag="PKN85_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNT44435.1"
                     /translation="MKKVFVGLTIGVLLTVLTVCHAAAQGPGPGHPGTGMDMDHPLWL
                     DLPHLTIDRNRMDAIREIEMRTVKEGIRKRAELQVASIDLRELLGEDPVDLKVVEAKL
                     KQIAALKMDLQFARIKAIEEIKGKLTSEERKKLREFLETAHMRSLPPRDRMIGPAPCD
                     QPQPPRSPVDGKSVPRKKEKVR"
     gene            1012..2400
                     /locus_tag="PKN85_08235"
     CDS             1012..2400
                     /locus_tag="PKN85_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44436.1"
                     /translation="MIRIGITYRLFLSILAATCAAILCMFLVMHWSINRGFLRYLDTM
                     AQAGMESVSMNLARAYASHGNWDFLRAEPAYWVTRALHEHDGPAPSGRQGQLHPPRLP
                     LVVLDANRRPLFGHPGKEDTIKYRPIIHSGSTVGYVGHLPPRQFLSPPQVQFLQHQKS
                     ALIAAVLGLVLVVVVFSLPLARRLIRPVRTIAAATRELASGRYAVRVPVTSSDELGQL
                     AHAFNAMAGALEKNEKARRQWVADISHELRTPLAVLRGEIEALMEGVRETTPEAVRSL
                     HSEVMRLHRLVDDLHQLTLSDLGALTYRKESVDLVEVLADSVDACEVEFDRKGIALEK
                     DIPRRQAAAHVFADRERLQQLFSNLLGNSLKYTDSGGRLVVRVRQDGATMTVDLEDSA
                     PAVPAGEMDRLFDRLYRVETSRNRASGGAGLGLTICRNIVEAHEGTITASPSPLGGLL
                     MRVSLPLVETGP"
BASE COUNT          475 a          811 c          722 g          519 t
ORIGIN      
        1 gggcttcgtc attaagagtg gtccaccagg cggccaggac ccccggatcc gcggcggcgg
       61 gtttgaggcc gcctttgagt gccgtgtgcc actgatcgga cacactgacg tcgggcctga
      121 cataatcagg cccgacgctc gcgcagccgc cgagaaggag caatccaaga agtgtgatcg
      181 gaagaaccgc cgtgaggtac agggcgggaa tatgctggga gttgatcttc atcggccgga
      241 tttgccttta agtatacttg aaaacgcccc tcaccgcacc ttttcctttt tccgtggaac
      301 actcttcccg tccacagggc tccttggcgg ctgaggttga tcgcatggcg cgggcccaat
      361 catcctgtcg cggggaggca gagacctcat atgggccgtc tcgaggaact ccctcagttt
      421 cttcctttcc tcgctcgtca gtttcccctt tatctcctcg atcgccttga tccgggcaaa
      481 ctgcaggtcc attttgaggg ccgctatctg tttcaacttc gcttcgacca cttttaggtc
      541 cacagggtcc tcacctagaa gctctctgag atcgatcgat gccacctgga gttccgccct
      601 cttcctgata ccctccttca cggtcctcat ctctatctcg cgtatcgcgt ccatgcgatt
      661 cctgtcgatg gtgagatgag gcaggtcgag ccagagggga tggtccatgt ccattcccgt
      721 gcccgggtgg cccggacccg gaccctgcgc ggctgcatgg cacacggtga ggaccgtgag
      781 gagcacacct attgtcaagc caacgaacac ctttttcata tctgcctcct tgattcgatc
      841 ttctgtatcc cgcgtctctc cccggtttgt ttcatgggca aagaatatcc gaagaatgtg
      901 gcaaaattgt gcaggaaata aggatattcg ccggaaagcg tcaaaaggct ccccatttcg
      961 ggagagtggt tgtagaatac atggcatggc acacaacgca agggattcgc gatgatacgg
     1021 atcgggataa cctaccggct cttcctctcc atcctcgccg caacgtgcgc ggcgatcctc
     1081 tgcatgttcc ttgtcatgca ctggagcatc aacaggggtt ttctccggta tctcgacacg
     1141 atggcgcagg ccggcatgga atcggtaagc atgaacctcg ccagggcgta cgccagccac
     1201 gggaactggg atttcctccg tgccgagccg gcgtactggg tgacgcgggc tctccacgaa
     1261 catgacggac cggccccttc ggggcggcag ggacaactgc atccgcccag actccctctt
     1321 gtcgttctcg atgccaaccg gcggccactc ttcggacacc ccgggaaaga ggacacgata
     1381 aagtacagac ccatcatcca cagcgggtcg accgtgggat acgtgggcca tcttcctccc
     1441 cggcagttcc tgagccctcc tcaggtccag ttcctccagc accagaagtc cgcccttatc
     1501 gcggccgtac tcggcctcgt tctggtggtc gtggtctttt cccttccgct cgcgaggcgg
     1561 cttatacgcc ccgtgaggac catcgcggcg gcaacccggg agctcgcctc gggaaggtac
     1621 gcggtacgcg ttcccgtaac gtcctcggac gaactgggtc agctcgcgca tgccttcaac
     1681 gccatggccg gcgccctcga aaagaacgag aaggcccggc ggcaatgggt ggccgacata
     1741 tcccatgaac tcaggacacc cctggctgtg cttcgcggcg agatagaggc cctcatggag
     1801 ggggtgcgcg agaccacccc cgaggccgtc aggtcgcttc acagcgaggt gatgaggctg
     1861 caccgcctcg tcgatgacct tcatcaactg accctgtccg atctcggcgc tctgacgtac
     1921 cgcaaagaga gcgtcgacct cgtggaggta cttgccgact ccgtcgatgc ctgcgaggtc
     1981 gagttcgacc gcaaaggcat agctctcgaa aaggacatcc cccgcaggca ggctgccgcg
     2041 cacgtctttg ccgaccgaga acgccttcag cagctcttct ccaacctcct cgggaactcc
     2101 ctgaaataca ccgacagtgg aggcaggctt gtcgttcgtg tccggcagga cggcgccacg
     2161 atgaccgtgg atctcgagga ttccgccccg gcggtgcctg ccggtgagat ggacaggctc
     2221 ttcgacaggc tctatcgcgt cgaaacctcc cgcaaccgcg catcgggagg cgccggactc
     2281 ggcctcacaa tctgcaggaa catcgtcgaa gcccatgaag gcaccataac cgcctctcct
     2341 tctccacttg ggggcctgtt gatgcgggtg tccctgcctc ttgtggaaac gggtccatga
     2401 acagggtcat cctcgtcgtc gaggacgaac cgaaactggc gagccttctg gccgattacc
     2461 tcagggcctc cagtttcgag ccccactgcc tcgcaaacgg catggacgtc gtcctctggg
     2521 tgcgcga
//
LOCUS       DAOQTR010000339         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_50001_length_2525_cov_3.395547, whole genome shotgun sequence.
ACCESSION   DAOQTR010000339 DAOQTR010000000
VERSION     DAOQTR010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50001_length_2525_cov_3.395547"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..501
                     /locus_tag="PKN85_08240"
     CDS             61..501
                     /locus_tag="PKN85_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44437.1"
                     /translation="MRKRPMPAAASVLIVVLLLLAAASVGAFPGEPDGYNGIPWGTPL
                     ESLTSMEYAGPGKDSPGTALYRRVTDDLTFGKARLTRIEYGFVAGRLTAVTLKVDSLL
                     QYLLMKEEAVRRFGPGTEADPRAERLIWEGDSTTIRLVSAFDLS"
     gene            714..1205
                     /locus_tag="PKN85_08245"
     CDS             714..1205
                     /locus_tag="PKN85_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNT44438.1"
                     /translation="MEILVLYHSRSGNTKKLAQAIAAGVESVTGASCVMKDTKEVGQE
                     DFLRADGIIAGSPVYFGSMAAELKEVFDTFVPVRKKMGDKVGAAFATSGDLSGGKETT
                     MMSIIQALLIYGMIVVGDPLDATGHYGVSCTGAPDEKTVENAGKLGKRVATLALALKK
                     RQA"
     gene            1377..>2525
                     /locus_tag="PKN85_08250"
     CDS             1377..>2525
                     /locus_tag="PKN85_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44439.1"
                     /translation="MKDQEISGKTEKGKRCGPDPAGEYQDVRALLEDSEARYRLLVKH
                     SSDGVFLFNPRTLKILEANASFLKTTGFREEEITRLTLLDITAMKKKEILQNVRGVLR
                     DAEIVSGLRQYRRKDGTLIDVEITSSLINYRNDQFIMVNVRDVTERLRAQHELERQAR
                     MLREQAEIIDIAEDAIIVRDMKNRVVFWNRGAANRFGFSMDEAWGKPLSSLLKTRYPE
                     PLSTIRKRLLAEGKWEGELVHRTKDGRRIIVESKWKLRTDKRNRPATVLQIDNDITER
                     KLAEEVLRRSKTELERRVAERTSEITEANARLVQELRRRQQVEDMLRRGAERYRNLFE
                     NSPLGIYRTDPQGKILMANPALIRMMGYSSFADFGAVKSYRKSCEPTYL"
BASE COUNT          582 a          689 c          811 g          443 t
ORIGIN      
        1 ctacgcggag agatacagga attgccccga catctgtgtc gtcgtggagg gggatgacga
       61 atgaggaagc gacctatgcc ggcggcggca agcgtcctga tagtggtgtt gttgctcctc
      121 gccgccgcga gtgtgggcgc gttccctgga gagccggacg gatacaacgg catcccctgg
      181 gggacacccc tcgagagcct gacgtcgatg gaatacgccg gcccggggaa ggactccccc
      241 ggcacagccc tgtatcggcg cgttacggac gacctgacct tcggcaaggc ccgcctgaca
      301 aggatcgagt atggattcgt cgccggaagg ttgacagctg ttaccctcaa ggttgactcg
      361 cttctgcagt atcttctcat gaaggaggaa gccgtgagaa ggttcgggcc gggcacggag
      421 gctgatccca gggccgaaag gctcatctgg gagggcgaca gcacgaccat aaggctcgtg
      481 agcgcctttg acctgtcctg agacggtgcc cacggggaag gattcctcga gataaaaaga
      541 aaatgaccag cgagcagatt ccaatggtca aagggatgcg ggaggcgata tgccgttccc
      601 tccgccggct tcccctccct gttcgtctat ccccgaacgg gggtacgggt acccccggca
      661 ctatctgatc attggagtcc ggtcatcggt cactatcgtc taaaggaggt ttcatggaga
      721 ttcttgtact gtaccattcg cgatcgggaa acacaaagaa gctggctcag gccatagcgg
      781 caggggtgga gtccgtcacc ggagcctcct gtgttatgaa agacacgaag gaggtcggac
      841 aggaggactt cctgcgcgcc gacgggatca tagccggttc cccggtctac ttcggctcca
      901 tggcagccga gctgaaagag gtcttcgaca cgttcgttcc cgtccggaag aagatgggcg
      961 acaaggtggg ggcggccttt gccacctccg gggacctttc gggaggaaag gaaaccacaa
     1021 tgatgtcgat catccaggcc ctgctcatct acgggatgat cgtcgtcggc gaccctcttg
     1081 atgccacggg ccattatggt gtatcgtgta cgggcgctcc cgatgaaaag acggttgaga
     1141 atgccggcaa gctggggaag agagtggcaa ccctggcgct ggcgttgaag aagcggcaag
     1201 cgtgagcgtt tcacgtggaa caccggcggg acgggctctg accgggtccg aaagccggaa
     1261 ggaccgatgc gcgatgcggg ggttttcctt cgggggatcc cccggcatcc ttttgtgatc
     1321 ccgacctggt gtctggcgcg caaaaatggt caatatcgat caggtgctga tataaaatga
     1381 aggaccagga gatatccggt aaaactgaga agggaaaacg atgcgggccc gacccggcag
     1441 gggaatacca ggacgtcaga gccctgctcg aggacagcga ggcccggtac cgtctgctcg
     1501 ttaagcactc gtcggacggc gtcttcctct tcaatccccg gacgttgaag atccttgagg
     1561 caaatgccag ctttctcaag acaaccggct tccgcgagga ggaaataacc cggctcacgc
     1621 tgctcgacat cacggccatg aagaagaagg agatacttca gaacgtccgg ggtgtgctca
     1681 gggacgccga gatcgtctcc ggactgcgcc agtacagaag aaaagacggc actctcatcg
     1741 atgtggagat cacgtcttcc ctcataaatt accgaaacga ccaattcatc atggtaaacg
     1801 tccgcgacgt cacggagcgg ctccgggcgc agcatgagct tgaacggcag gcgagaatgc
     1861 ttcgggagca ggcggagatc atcgatatcg ctgaagatgc catcattgtc cgcgacatga
     1921 agaaccgggt agtcttctgg aatagggggg cggcgaaccg cttcggcttt tcgatggacg
     1981 aggcctgggg aaaaccgctg tcgtcgcttc tcaagacgcg gtacccagaa ccactcagca
     2041 ccatacggaa acgccttctg gccgagggaa aatgggaagg ggagctggtc catcgtacga
     2101 aggacggcag gcggatcatc gtggagagca aatggaagct caggacggac aagagaaaca
     2161 ggcccgcaac ggtgcttcag atagacaatg acatcacgga gcggaagctc gccgaggagg
     2221 ttttgaggcg ctcaaagacg gaattggagc ggcgcgtggc ggagcgaacg agcgagataa
     2281 cggaggccaa cgcccgcctc gtgcaggaac tgagaagacg ccagcaggtc gaggacatgc
     2341 tgagaagggg ggccgagcgt tacaggaacc tcttcgagaa ctcaccgctc ggcatatacc
     2401 ggacagaccc gcaggggaag atcctcatgg cgaacccggc cctgatccgc atgatgggct
     2461 attcctcctt cgccgacttc ggagccgtca agtcctaccg aaagagctgt gagcccacct
     2521 acctc
//
LOCUS       DAOQTR010000340         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_50040_length_2524_cov_3.144593, whole genome shotgun sequence.
ACCESSION   DAOQTR010000340 DAOQTR010000000
VERSION     DAOQTR010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50040_length_2524_cov_3.144593"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKN85_08255"
     CDS             complement(<1..499)
                     /locus_tag="PKN85_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44440.1"
                     /translation="MTDFPSYVRLHEEGVLGDRLEQILGLASPCMLCPRQCMVDRTTG
                     DRGYCQAPYGLYVSSASPHFGEEGPLVGTGGSGTIFMTHCNLRCVFCQNHDISIRGEG
                     VSCSAAALADVMTDLQRKGCHNINLVTPTHYVHQIVRALPSAIEKGLTIPLVYNSGGY
                     DSPEVL"
     gene            569..841
                     /locus_tag="PKN85_08260"
     CDS             569..841
                     /locus_tag="PKN85_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44441.1"
                     /translation="MKKFEYTVRIYSMEELKRSGVDIEEENNIVYACRPGGECEVHDV
                     GAEQLDNLSGLLNEMGASGWELVELIFHQSGIVSFWKRETADRESS"
     gene            879..1358
                     /locus_tag="PKN85_08265"
     CDS             879..1358
                     /locus_tag="PKN85_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT44442.1"
                     /translation="MWETKFPSLREEMDRVFEEFFGEAGFPTLREADWLPAVDVVERK
                     GEIVVIMDIPAIDPAEVKITILEDKLTVEGERKREEEFREEDYYRSERVHGSFLRSIQ
                     LPADVIGEKASAVYNGGVLTITVPKSRRPEAREIKVSVQEARPVEAPVSARQRRKKT"
     gene            1355..1573
                     /locus_tag="PKN85_08270"
     CDS             1355..1573
                     /locus_tag="PKN85_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNT44443.1"
                     /translation="MKEKVQQALEKIRPLLQRDGGDIQLIDVVDGIVKVKLQGACGSC
                     PMSMMTLKMGVEKQLKQEIPEVKEVVSV"
     gene            1634..>2524
                     /locus_tag="PKN85_08275"
     CDS             1634..>2524
                     /locus_tag="PKN85_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44444.1"
                     /translation="MSPEKRLIIIDDEVTLLAVLQEFLQQFGLEVFTYQSMPDLRAEL
                     GDKRPHAVLCDIVLPGISGIEILKEIKKIDRRIPVIMMTGYAEERERLESLSNGAYAL
                     LTKPFKSFEELYHIVNNSMDHYREVLRTEDLTAQVEERFRREKINILELEFLKNLQRM
                     IGETEDPVFVLRNAHTLLGSFLDFQFFGTMLVGGQEADIHIFPETGGDPELRRSMVSA
                     LAGWGNRVPGQISPGTASVVSELRAAKRLYGYAALYRQEPFNEEETSIFTRFCSHIAF
                     TLEKISLFEEIRTLSRHDGLT"
BASE COUNT          611 a          657 c          780 g          476 t
ORIGIN      
        1 gaagaacctc cggagagtcg tatcctccgc tgttgtagac gagggggatg gtgagaccct
       61 tctctatggc cgaggggagg gccctgacga tctggtggac gtagtgcgtg ggggtgacaa
      121 ggtttatgtt atgacaacct tttcgctgga ggtccgtcat gacgtcggcg agggcggcag
      181 ccgaacagct gacaccttct ccccggatgc tgatgtcgtg gttctggcag aagacgcacc
      241 tgagattgca gtgcgtcatg aatatcgtcc cggaaccgcc cgttccgacg agaggcccct
      301 cctctccgaa atgcggtgag gcggaggaaa cgtaaaggcc gtacggggcc tggcagtaac
      361 ccctgtcacc ggtcgttctg tcgaccatac actgcctggg gcaaagcatg caaggtgacg
      421 caaggccgag tatctgctcc aacctgtcgc ccagcacgcc ctcctcgtgc agcctcacgt
      481 aagacggaaa atcggtcatg acactttaaa aatatcacac aatggtttac tataatagag
      541 caaaggacac ggagcaggag gtagacccat gaagaaattc gaatacaccg tccggatcta
      601 ctccatggaa gagctcaagc gaagcggcgt ggatatagag gaggagaaca atatcgtcta
      661 cgcctgcagg ccggggggcg agtgcgaggt ccacgatgtg ggggccgagc agctcgacaa
      721 tctttccggt ctgctcaatg agatgggagc ttcggggtgg gaactcgtgg agctcatttt
      781 ccaccagtca ggcattgtca gcttttggaa gagagaaacc gccgaccggg aaagttcata
      841 acaggaggta tcagatgcct ttgacgccgt ggaagtctat gtgggaaaca aagtttccct
      901 ctctcaggga agagatggac cgggtgttcg aggaattctt cggcgaagcc gggtttccca
      961 cgcttcgcga ggccgactgg cttccggccg ttgacgtcgt ggagaggaaa ggcgaaatcg
     1021 ttgttatcat ggacataccg gcgatagacc cggcggaagt gaagatcacc atcctcgagg
     1081 acaagctgac cgtcgagggt gaacggaaac gggaggagga gttccgcgag gaggactatt
     1141 accgctccga aagggttcat ggatctttcc tgaggagcat ccagctcccg gcggacgtca
     1201 ttggcgaaaa ggcctcggcc gtatacaacg ggggtgttct taccatcacg gtacccaaat
     1261 cacggaggcc ggaggcgaga gagataaagg tcagcgtaca ggaggcgcgg cctgtcgagg
     1321 caccggtcag cgccaggcaa cggaggaaga agacatgaaa gagaaagtcc agcaggcgct
     1381 agagaagatc aggccccttt tgcagaggga cgggggagat atccagctta tcgatgtggt
     1441 tgacggcatc gtgaaggtca agctgcaggg cgcgtgcgga agctgcccca tgagcatgat
     1501 gaccttgaag atgggcgtgg agaaacagct caaacaggag atcccggagg tgaaagaggt
     1561 cgtttccgtt tgacgggccc cgcaatgcat gagtcgaagg gtggatcgac ggatctgaaa
     1621 ggtccggttc agaatgtcac ctgaaaaacg tctgatcatc attgatgatg aagtgacgct
     1681 tcttgccgtg ctgcaggaat tcctgcagca gttcggactc gaggtcttca cctaccagag
     1741 catgcccgac cttcgcgcgg agctcgggga caagaggcct cacgccgtgc tgtgcgacat
     1801 cgtcctcccc ggcatctcgg gaatagagat cctcaaggaa ataaaaaaga tcgaccgccg
     1861 tatacccgtt ataatgatga cggggtacgc ggaggaaagg gaaagacttg aatccctgag
     1921 caacggagcc tacgcgctgc tcacaaaacc cttcaagagc tttgaggaac tctaccacat
     1981 cgtgaacaac agcatggacc attacaggga ggtactcagg acggaggatc tgacggcaca
     2041 ggtcgaagag cgcttccgcc gcgaaaagat caacatcctc gaactcgagt tcctcaagaa
     2101 cctccagcgc atgataggcg agaccgagga ccccgtgttc gtgctcagaa acgcgcacac
     2161 gctgctcgga agtttcctcg atttccagtt tttcggtacc atgctcgtgg gcgggcagga
     2221 ggccgatata cacatcttcc ccgagaccgg cggcgatccg gaactccggc ggtcaatggt
     2281 ctccgcgctt gcaggctggg gaaaccgggt gcccggacag atctctcccg gcaccgcgtc
     2341 ggtggtctcg gagctcaggg ccgcaaaacg tctttacggg tatgccgcgc tatacaggca
     2401 ggagcctttc aacgaagagg agacgtcgat attcacccgt ttctgttccc acatagcctt
     2461 caccctggag aagataagcc ttttcgaaga gatcaggacc ctctcccggc atgatgggtt
     2521 gaca
//
LOCUS       DAOQTR010000341         2522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_50094_length_2522_cov_3.745034, whole genome shotgun sequence.
ACCESSION   DAOQTR010000341 DAOQTR010000000
VERSION     DAOQTR010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50094_length_2522_cov_3.745034"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKN85_08280"
     CDS             <1..533
                     /locus_tag="PKN85_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT44445.1"
                     /translation="CRRGMSRSFQVVNVFPRLTVFENVQVSVLAKQKRTWNLFAPSAK
                     LAVSETEKILENVGLADIKDATSAALSHGERKVLEIAIALGGSPEFLILDEPTAGMSP
                     EETTRCIDLIKQLKEKLGITILFCEHDMEIVFSISDRIMVMVRGSTIIEGTCDDVRCC
                     QEVQDAYLAGSDACLT"
     gene            521..1210
                     /locus_tag="PKN85_08285"
     CDS             521..1210
                     /locus_tag="PKN85_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44446.1"
                     /translation="MLDVNAIDTYYGLSHILFGVTLSVRAGEVVGLLGRNGAGKSTTM
                     KSIMGIVPPRKGTITFKGAGIAGRKPYQLFRQGIGYVPDDRRVFADLCVDDNLEIVHR
                     RNDEWDKERVYGLFPALKEIRSRRAGHLSGGEQQMLTIARALMGSPELLLLDEPTEGL
                     APLIVRDLEEQIVRLKEAGISILLSEQNVRSALKMITRAYVIDNGRIRFEGTVQELEA
                     NEEVKRKYLMI"
     gene            1430..1996
                     /locus_tag="PKN85_08290"
     CDS             1430..1996
                     /locus_tag="PKN85_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44447.1"
                     /translation="MFMTLARAWKLCFIGLFVLVFGVSSVHAVGDLGIVVEKLKDPEK
                     TLFCPVCGRAISPGVVHTEATAIIREKLKTALTDRNIGYSDGMKQDQPYIQVLVYRFQ
                     ERKGGNFAVEKPASVAFHMHLMKNGVVGKIFAYSEEQKALMQNLLTVGKFFSRGARWV
                     TAEELAGEGINAGLDELVEPKETTEPAE"
     gene            1993..>2522
                     /locus_tag="PKN85_08295"
     CDS             1993..>2522
                     /locus_tag="PKN85_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNT44448.1"
                     /translation="MKALFAMDLMGGKTVRLRKGDFQAVTVYSEDPPSLIEEMVHRGA
                     RDFHIIDLDGARTGEPVHGEIIKRIRSTVKGYMEVGGGIRTDDTIKYYSDLGIDGIIV
                     GTRALEDEEYFEGLSRFPNIVLGLDLLGGKPMSRGWKSAIDRDPATILKAAERIGIMA
                     VLCTSIERDGMLTGPDL"
BASE COUNT          532 a          717 c          780 g          493 t
ORIGIN      
        1 tctgccgccg gggtatgtcg cggtccttcc aggtggtgaa cgtctttccc cggctcaccg
       61 tgttcgagaa cgtccaggtc tcggtgctcg cgaagcagaa gaggacctgg aacctctttg
      121 ccccttccgc gaagctcgcc gtaagcgaga cggagaagat cctcgagaac gtgggtcttg
      181 cggacataaa ggatgcgacg agcgccgccc tctcccacgg tgagcgcaag gtcctggaga
      241 tagccattgc cctcgggggc agccccgagt tcctgatcct cgacgaaccc acggcgggca
      301 tgtcgccgga ggagacgaca cgctgcatcg acctcatcaa acagctcaag gagaagctcg
      361 gcatcaccat ccttttctgc gagcacgaca tggagatcgt gttctccatc tcggaccgga
      421 tcatggtcat ggtccggggg tcgacgatca ttgagggcac ctgcgacgac gtcaggtgct
      481 gccaggaggt gcaggacgca tatctcgccg ggagcgacgc atgcttgacg tgaacgccat
      541 cgacacctac tacggattga gccatatcct cttcggcgtg accttgagtg tcagggccgg
      601 cgaagtcgtc ggccttctcg gtcgcaacgg tgcggggaag tcgaccacca tgaagagcat
      661 catgggaatc gtgccgccgc ggaaggggac gatcaccttc aagggggccg ggatcgccgg
      721 gcgcaaacct taccagcttt ttcgtcaggg tatcgggtat gtccccgatg accgcagggt
      781 cttcgccgac ctgtgcgtcg acgacaacct ggagatagtc caccggcgca acgacgagtg
      841 ggacaaggaa agggtgtatg gtcttttccc cgccctgaag gaaataaggt cgcgtcgcgc
      901 cgggcacctc tccggcgggg agcagcagat gctcaccatc gcgcgggcgc tcatggggag
      961 ccccgagctc ctgctcctcg acgagcccac ggagggcctc gccccgctca tcgtccggga
     1021 cctggaagag cagatcgtaa ggctcaagga agccggcatc agcatcctcc tgtcggagca
     1081 gaacgtccgc tccgccctca agatgatcac cagggcctac gtcatcgata acggcaggat
     1141 ccgcttcgaa ggcaccgttc aggaactgga agcgaatgaa gaggtgaaac ggaagtacct
     1201 tatgatctag ttttcggctc cgcgaaaacc agatccgttt taggttttaa gttttaggtt
     1261 ttaagcaaaa gacaaaaaga ccgtttcagg tttcaccttt caggtgagaa ccgaaaaaag
     1321 gcggatatgt ttcccggtct ttggcctgaa acctgaaacg tgagacctga gcccgttttc
     1381 actctctttt tgtctttctt ttggtccggt tttcctatag aatttataca tgttcatgac
     1441 tttggcgaga gcctggaagc tttgtttcat cgggttgttc gtgctcgtct ttggcgtttc
     1501 gtccgtccat gccgtgggcg acctcggcat cgtcgtggag aagctcaagg atccggagaa
     1561 aaccctgttc tgccccgtgt gcggcagggc gatcagcccc ggtgtcgttc atactgaggc
     1621 cacggccatc atcagggaga agctcaagac tgcgctcacg gaccggaaca tcggatactc
     1681 ggacgggatg aagcaggacc agccttacat acaggtcctt gtctatcgtt tccaggagcg
     1741 caagggtggt aatttcgccg tggaaaaacc cgcctccgtt gcctttcata tgcacctcat
     1801 gaagaacggg gtggtgggca agatattcgc ttactcagag gagcaaaagg ccctcatgca
     1861 gaacctgctg acagtgggga agttctttag caggggggcg cgctgggtga cggcggaaga
     1921 gcttgccggg gaaggcatca acgccggact ggacgaactc gtcgagccga aggagacgac
     1981 ggagcccgcg gagtgaaagc gctctttgcg atggacctga tgggcggcaa gaccgtgcgt
     2041 ctcaggaagg gtgatttcca ggcggtgacg gtttacagcg aagacccgcc ctcgctgatc
     2101 gaggagatgg tccaccgcgg cgcccgggat ttccatatca tcgatctcga tggcgcaagg
     2161 acgggcgagc ccgtgcacgg ggagatcatc aaaaggatcc gttccacggt gaagggatac
     2221 atggaggtcg gcggcggcat ccgcaccgac gataccatta aatactacag cgatctcggc
     2281 atcgacggca tcatcgtcgg cacccgcgcc ctggaggatg aggagtattt cgaagggttg
     2341 tcacggttcc ccaacatcgt cctcggcctc gaccttctcg ggggcaagcc gatgtcgcgg
     2401 ggatggaaga gcgccatcga cagggacccc gcaaccatcc tcaaagccgc cgagcgcatc
     2461 gggatcatgg ccgtcctgtg caccagcatc gagcgcgacg ggatgctgac cggacctgat
     2521 ct
//
LOCUS       DAOQTR010000342         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_50124_length_2521_cov_3.311436, whole genome shotgun sequence.
ACCESSION   DAOQTR010000342 DAOQTR010000000
VERSION     DAOQTR010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50124_length_2521_cov_3.311436"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..875)
                     /locus_tag="PKN85_08300"
     CDS             complement(45..875)
                     /locus_tag="PKN85_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT44449.1"
                     /translation="MKLKRLKKRWMTSVTILVVPHSKSRPLKLRLPVAGLMACMVLAV
                     IGAVYITIAGVKTAEYYVMKTRLSYFTREFNALKQNITSLKETNNELSRLVELKSKNS
                     ILKSAEFSDSGSIDIEAIKKEMMETIESVAEIKKYITEQKNIYLATPRGWPIQGQISS
                     GFGMRHHPVTGVRSHHSGLDIRAPRGTPVKVTADGIVTYSGWSGGNGHIIVIEHGHGF
                     STAYAHNKENLVTVGQTVRKGQDIALTGSTGMSTGPHLHYEVWRNGRPVDPAHYLKDG
                     "
     gene            1161..1394
                     /locus_tag="PKN85_08305"
     CDS             1161..1394
                     /locus_tag="PKN85_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44450.1"
                     /translation="MKKCSAAVLSFLVIVLAASAGFASDDLKIVKDKEGTTWSVGSDE
                     SRERARQEEERDRDRAWDMLRNQSIIIDKRTTK"
     gene            1397..>2521
                     /locus_tag="PKN85_08310"
     CDS             1397..>2521
                     /locus_tag="PKN85_08310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT44451.1"
                     /translation="MEKGRVIIVEDEENILSMLGEFLMANGYAVDPYIDSRKALSALK
                     NNGYHVLITDLMMPKVDGLQLINFIQKEYLDTLGIVMTGYGSMESAISAMRCGAFDYI
                     LKPFKFEEVLATVDSAMYYFNLLKSDNIPKGLTLKANLLRRYSENKMVRENTILRSAL
                     KDKYKFENIIGVSFPMQKVFEMIERVADTSATVLITGESGVGKELVARAIHYNSSKRD
                     NPLVVVNCGAIPETLLESELFGYEKGAFTGAVNTRLGRFELAAGGSIFLDEIGDMSFN
                     LQVKLLRVLQEKSFERIGGSKTIKVDVRIIAATNRKLEDLVSENKFREDLYYRLNVVP
                     IEIPPLRERRQDIPLLVNFFLEQSNRINSAAIEGFSEEAMK"
BASE COUNT          550 a          666 c          719 g          586 t
ORIGIN      
        1 gagggtctcc agggaccccg cttcatgctt cttgtttgcc attgttatcc gtccttgaga
       61 tagtgagcgg gatcgacagg cctgccgttc ctccacacct cgtagtggag atgaggaccc
      121 gtcgacatgc ccgtcgaacc ggtaagtgcg atgtcctgtc ctttcctgac cgtctgaccg
      181 acggtgacga ggttttcctt gttgtgcgcg taggcggtgg agaatccatg gccatgttcg
      241 atgacgatga tgtggccgtt gcctcccgac catcctgagt aagtgacgat gccgtctgcg
      301 gtgaccttga cgggtgttcc gcggggcgcg cggatatcaa gccccgaatg gtgtgaccgt
      361 acccctgtga cgggatggtg acgcattccg aaaccggagc tgatctgccc ctgaatgggc
      421 catccccggg gagtcgcgag atatatgttc ttctgttcgg tgatgtattt cttgatctcc
      481 gccaccgact ctatggtctc catcatttcc ttcttgatgg cctcgatgtc tatcgacccg
      541 gaatcgctga attcggcgga cttgaggatg ctgttcttgg atttgagttc cacaagccgc
      601 gaaagttcgt tgttggtctc cttgagcgag gtgatgttct gcttgagagc gttgaactcc
      661 ctggtaaaat aggagagcct cgtcttcatg acataatatt ccgctgtctt gaccccggcg
      721 atcgtgatgt acacggcccc gatgacagcg aggaccatgc aggccatgag gccggccacg
      781 ggcagcctta gcttcagggg ccgggatttg gagtggggga cgaccagaat ggtaacggac
      841 gtcatccatc tttttttcag acgcttcagt ttcatacgtg atgttcctga acttgcaaac
      901 cttataccgt aatcggcaaa cctgtcaagt ccttaacttg gggggcaact tttcgccgat
      961 atcaggcatc ttccggaccg tcgaagaggg ggatggcttc atgagcgcat catcaggatt
     1021 gtcgcaggta cttgtttggc agaaggtccg tttcatcggg agggggctat ttttttctta
     1081 ctttatccca aaaaattggc tatatatatt ggtatgttca caccggtacc attaatgacc
     1141 tgtgcaacga gatgtgcgtg atgaagaaat gcagtgcggc ggttctctcc tttctcgtga
     1201 tcgttctggc ggcgtcagcc ggctttgcct ccgatgatct caagatcgtc aaggacaaag
     1261 aaggcaccac ctggtctgtc ggttccgacg aatcacgtga aagggccagg caggaggaag
     1321 agcgcgacag ggatcgcgcc tgggacatgc tcaggaacca gtcgatcata atcgacaagc
     1381 ggacaacgaa ataaggatgg aaaaaggaag ggtcatcatt gttgaggatg aggagaatat
     1441 cctctccatg ctcggtgagt ttctcatggc caacgggtat gccgtcgatc cctacatcga
     1501 cagcagaaag gccctttcgg cgctgaagaa caacggctac catgttctga taacggacct
     1561 catgatgccc aaggtcgacg gtcttcagct tattaacttt atccagaaag aatacctcga
     1621 caccctcggc attgtcatga ccgggtatgg cagcatggaa agcgccataa gcgccatgcg
     1681 gtgcggtgcc ttcgactata tcctcaaacc cttcaagttc gaggaagtcc tcgcgacagt
     1741 ggattcggcc atgtactact tcaatctcct caagagcgac aacatcccca agggtcttac
     1801 cctcaaggcg aatctcctga ggcggtattc cgagaacaag atggtccgtg agaacaccat
     1861 tctgaggtcg gccctcaagg acaagtacaa gttcgagaac atcatcgggg tgagctttcc
     1921 catgcagaag gtcttcgaga tgatcgagcg cgtggccgac accagcgcca cggtgctgat
     1981 caccggtgag agcggtgtgg gcaaggaact cgtggcgagg gccatccatt acaattcgtc
     2041 gaagcgggac aaccctctcg ttgtggtcaa ttgcggcgcc attccggaga ccctcctgga
     2101 gagcgagctt ttcggctatg agaagggcgc tttcacgggt gccgtgaata cccgtctcgg
     2161 caggttcgag ctcgcggcgg gcggctcgat ctttctcgat gagatcgggg acatgagctt
     2221 caacctgcag gtcaagctcc tgagggtcct ccaggagaag tccttcgagc gcatcggcgg
     2281 ctcgaagacg atcaaggtcg acgtgaggat catcgccgcc acgaaccgca agctggaaga
     2341 cctcgtcagt gagaacaagt tccgtgagga cctgtactac cgtctcaacg ttgttcccat
     2401 cgagatcccg ccgctcaggg agaggcgtca ggacatcccg ctgctcgtca atttcttcct
     2461 cgaacaatcc aacaggataa actcggcggc catcgagggc ttttcggaag aggccatgaa
     2521 g
//
LOCUS       DAOQTR010000343         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_50480_length_2509_cov_2.982885, whole genome shotgun sequence.
ACCESSION   DAOQTR010000343 DAOQTR010000000
VERSION     DAOQTR010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50480_length_2509_cov_2.982885"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..448
                     /locus_tag="PKN85_08315"
     CDS             134..448
                     /locus_tag="PKN85_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44452.1"
                     /translation="MTRQDILFEPGWTPYRELEIEAGIAMLREIWGGEFSPDYIRGAV
                     EMLRRIIMLPQAMAATPEAKERAEALVKASFSAMELKLLRRAMFDSEQPAAMPSKQEK
                     GE"
     gene            451..1314
                     /locus_tag="PKN85_08320"
     CDS             451..1314
                     /locus_tag="PKN85_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44453.1"
                     /translation="MPEDERDLLNENDSGVSGDDDGDLLSVVSLEGDEEGGEESGSGE
                     GEKKKADAGKDLDPEMLSAQLVRAQEHINNLNKALHEERQSKKKAAEKGGEPAFTKAQ
                     LKELWTEHRDDPDVLFNILSYMADETARSAQAKAVDQVELVSKKKEVDDYLAQNFPDL
                     ANDGSPLRVGVDKAKQELMLADHPFGDILALGLNNLLNLPGTVRAAYEMGKKEAQGGK
                     QMDRRKEKINASNLPKGKTHKVDADDRHTEPASPEVRDVAKRIGLSKQGQEIYQRILK
                     NAKTRSVTVEG"
     gene            1318..1983
                     /locus_tag="PKN85_08325"
     CDS             1318..1983
                     /locus_tag="PKN85_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44454.1"
                     /translation="MRTKKAAVAKQTDPVADQVPVQPVDSSQLTPEELAIARRVAAEG
                     PILDHGEEGMVDFSLAEDPLKLPEPALKEQREKRLAFRWIRRSPERIDQVRNASPPLR
                     WWLCNRVNTPFLAKYVDPILGAVVRLDLILVCKPWAHFMAEQKAKLELADMGTASTIK
                     AKDGIRDGKDDHYEWKGGMRPESSTDRLAAELHRAPLLEEGKDFLQGGSMDAAGGDDI
                     IAD"
     gene            1997..>2509
                     /locus_tag="PKN85_08330"
     CDS             1997..>2509
                     /locus_tag="PKN85_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44455.1"
                     /translation="MANTDAPFGFIPYGKLLSADWYPVATAYGTAIYVGDWVEITNTG
                     LVCKVFDGDTRLGVEIDATGAAGDELGAVLAILDSNGDPLKYLPASTTGDGVVAGYVL
                     VADHPLQEFLVQEDGVNTPIAAASLGLNVAMISTHSGSTTTGRSKQEIDSDSVNTTNT
                     LALRIVKSYGG"
BASE COUNT          601 a          733 c          805 g          370 t
ORIGIN      
        1 acatcaaccc cgcagtgaat atgatcctgg aagccctgaa gcaggccccg gaagttgttc
       61 aggtgatcca gcagtacatg cagacgaaag ctgaaaccca gcggctcgtc gaaggggtcg
      121 ggcaggaggc ggcatgacca ggcaggacat ccttttcgag cccggatgga ctccgtacag
      181 ggagctcgag atcgaggcgg gaatcgccat gctccgggag atctggggcg gcgagttctc
      241 gccagactat atccgggggg ccgtcgagat gctgcggcgt atcatcatgc tcccgcaggc
      301 catggcggcg accccggagg caaaagaacg ggccgaggcc ctcgtcaagg cctcattctc
      361 cgccatggag ttaaagctcc tgcggcgagc catgtttgat agtgaacagc cggcagcaat
      421 gccgagtaaa caagagaaag gagaatgatc atgccagaag atgaacggga tctcctcaac
      481 gaaaacgact ccggcgtatc cggagatgat gatggagatc tgctctccgt cgtgtccctc
      541 gagggtgacg aagaaggcgg agaagaaagc ggatccggtg aaggcgaaaa gaagaaggcc
      601 gacgccggca aggacctgga ccccgagatg ctcagcgcgc agcttgtcag ggcccaggaa
      661 cacatcaaca atctcaacaa ggcactccac gaggagcggc agtcgaagaa gaaggcggcc
      721 gagaagggcg gggaaccggc cttcacgaag gcccagctca aggaactgtg gacggagcat
      781 cgtgacgatc cggacgtttt gttcaacatc ctcagctaca tggctgacga aacggcccgc
      841 agcgcacagg caaaggccgt ggaccaggtc gagctcgtct ccaagaaaaa ggaggtcgac
      901 gactacctcg ctcaaaactt tccggatctc gcaaacgacg gatcgccgct acgggtcgga
      961 gtcgataagg ccaagcagga gctcatgctg gccgatcatc cattcggtga cattctggcc
     1021 ctcggcctca acaacctcct gaacctgccg ggcaccgtca gggcggcata tgagatgggg
     1081 aagaaggagg cccaaggagg gaagcagatg gaccgcagga aggaaaagat caacgcaagc
     1141 aatctgccga agggcaagac ccacaaggtt gatgcggatg atcggcacac ggagcccgcg
     1201 agccccgagg tgagggacgt tgcgaagcgg atcggactgt ccaagcaggg acaggagatc
     1261 taccagcgga tcctcaagaa cgccaagacc cgttcagtaa ccgtggaggg ataagacatg
     1321 agaacgaaaa aagcggcagt tgcaaaacaa accgatcccg ttgcggatca ggttcccgtc
     1381 caaccggtgg acagctccca gctcacgccc gaggagctgg ccatcgcgag acgagtagcc
     1441 gccgagggcc cgatcctgga ccacggcgaa gagggaatgg tggatttctc cctggcggaa
     1501 gacccgctga agctccccga gcctgctctc aaggagcaga gagaaaagag acttgcattc
     1561 cggtggatcc gcagaagccc ggagcgaatc gatcaggtgc gaaacgcctc ccccccgctc
     1621 cgctggtggc tctgcaaccg agtgaacacg cctttcctgg cgaaatacgt cgatccgatc
     1681 ctgggcgccg tcgtgcgcct ggacctgatc ctggtctgca aaccgtgggc ccatttcatg
     1741 gccgagcaaa aggccaagct ggagctggcc gacatgggaa ccgcgagcac gatcaaagcg
     1801 aaagacggga tccgggacgg gaaggatgac cattacgaat ggaaaggcgg catgaggccg
     1861 gagagctcca cggacaggct cgcggccgag ctgcacaggg ccccgctcct cgaggaggga
     1921 aaggacttcc tccagggcgg atcgatggat gcggcgggag gcgatgacat catcgctgac
     1981 taaggagtaa ctcatcatgg caaatactga cgcccccttc gggttcatcc cgtacggaaa
     2041 gctgctctcc gccgactggt atccggtggc gacggcctac gggacggcga tctatgtggg
     2101 cgactgggtc gagatcacca ataccggcct ggtctgcaag gtcttcgacg gcgacacgcg
     2161 cctcggcgtc gaaattgatg caaccggcgc agccggcgac gagctgggag cggtcctggc
     2221 gatcctcgat tccaacgggg atccgctgaa gtacctcccg gcctccacga ccggcgacgg
     2281 cgttgtggcc ggctacgttc tcgtggccga tcacccgctc caggagttcc tcgtccagga
     2341 ggacggggtc aacactccca tcgcggcggc gagcctcggg ctcaacgtgg ccatgatctc
     2401 gacccactcc gggtccacga ccacgggccg cagcaagcag gagatcgatt cggactccgt
     2461 gaacacgact aacactctgg ccctccggat tgtcaagtcg tacggcgga
//
LOCUS       DAOQTR010000344         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_51066_length_2491_cov_3.668309, whole genome shotgun sequence.
ACCESSION   DAOQTR010000344 DAOQTR010000000
VERSION     DAOQTR010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51066_length_2491_cov_3.668309"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="PKN85_08335"
     CDS             complement(<1..1473)
                     /locus_tag="PKN85_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UUP1 family membrane protein"
                     /protein_id="HNT44456.1"
                     /translation="MKRPALAAALALIAISLIIVTYRIVSLGYPLTPARPDYVWTFRF
                     DGVLQGTGKDPLFLLSLPSQEHGQIILDESIGSGAMNFNLLKEYDNRIGVWSGRPQRD
                     GESISYRATILFQHKGSDGTVMPPRSSYPYGVADDQIAPIEQLTGQWRTMALSTRIQV
                     VLGFARVGAAGSGNDPNLMKLDETLEGYDDITRLLILLAAVNVPARTVEGVDLVEGVV
                     GSPFPWVEAWTGQSWEHIDPDRMEVIPSARKFLPLSVGGMSAIRISAGKLPMKRFELT
                     RNVVGRWRIFFERVSRSNRVLDVWSLFRIPPEFQQTFRILLLVPLGALLIALLRNIVG
                     FQTFGIFMPVLMALAFRSTGLIYGLGIFAAIIAIGYAARRYLNRMRLLLVPRMSVLVS
                     LVIFCFVILALVGNRLGLRQFMAVGLLPFVILTMTIERFFVVVEEHGMKTALQTTAGS
                     AAVAVITYLIIQWEVLQITFFVYPELILFVMGLQILVGRYT"
     gene            complement(1470..2000)
                     /locus_tag="PKN85_08340"
     CDS             complement(1470..2000)
                     /locus_tag="PKN85_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HNT44457.1"
                     /translation="MTAKDILREISNHASCFIIPALLFLTCAFPDHTLGEPDKPVIGG
                     EEYVRILPYGFTVPARIDTGAATTSLDARNIRVEESTVTFALPPKWGGATIKARIVDW
                     KYVRTSKSREKRPVIELELCIASKQLRARVNLNDRSRMRYPLLIGRNVITGNFLVDTS
                     QSFWNSPETEDTEVDR"
     gene            2096..>2491
                     /gene="amrB"
                     /locus_tag="PKN85_08345"
     CDS             2096..>2491
                     /gene="amrB"
                     /locus_tag="PKN85_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT44458.1"
                     /translation="MTTVRLPSVSGMFYPEDPVLLRKDIEAYLKGAEVEPISGRVHAI
                     VSPHAGYVYSGQVAAFAYKAVSGLEVDSVIVIAPSHRAYFEGIALWETGSFRTPLGDI
                     RIDEKGAGELLETRDVFTANRNVHKAEHSL"
BASE COUNT          537 a          680 c          786 g          488 t
ORIGIN      
        1 ggtgtaccgg cccacgagga tctgaagccc catcacaaac agaatgagct cgggatagac
       61 gaagaaggtg atctgaagga cctcccattg tatgataagg tacgtgatca ccgcgaccgc
      121 ggcgctaccg gcagtcgtct gcagcgccgt cttcatgcca tgctcctcga cgacaacgaa
      181 gaagcgctct atcgtcatgg tgagaatgac gaagggaaga agaccgacgg ccatgaactg
      241 tctgagcccg agtctattgc ccacgagagc caggatgaca aagcagaata tcacgagact
      301 tacgaggacg gacatcctgg ggacgagaag cagccgcatc ctgttcaggt atcgtcgggc
      361 cgcgtatccg atagcgatta tcgccgcgaa gatgccgagg ccgtagatga gaccggtgct
      421 tcggaaggcg agggccatca acacgggcat gaatatgccg aaggtctgga agccgacgat
      481 gttgcgaagc agggcgatga gcagggcgcc cagcggcaca aggagcagta ttctgaaggt
      541 ctgctggaat tcaggcggga tcctgaaaag ggaccacacg tcgaggaccc tgttggaccg
      601 gctgaccctc tcgaagaaga tgcgccaccg gcccacaaca ttccgggtca gctcaaatcg
      661 cttcatcggg agcttgccgg cggagatcct gatagccgac atccccccaa cgctcaaggg
      721 gaggaatttc ctcgccgagg ggatcacctc catgcggtcg ggatctatgt gctcccagct
      781 ttgtcccgtc caggcctcga cccaggggaa tggggagccc acgacgccct ccacgaggtc
      841 cacgccctcg acggtccgtg ccgggacatt tacggcagcc aggaggatga gaagcctcgt
      901 gatgtcgtcg tacccttcca atgtctcgtc gagcttcatg agattggggt cattgccgga
      961 gcccgctgcg ccgaccctgg caaatcccag gaccacctgt atccgggtgg agagggccat
     1021 ggtgcgccat tggcccgtca gctgctcgat gggggctatc tgatcgtctg cgaccccgta
     1081 aggataggaa ctccggggcg gcatgaccgt tccgtcagat cctttgtgct ggaagaggat
     1141 ggtggcccgg tacgatatgc tctcaccgtc cctctgcggc ctgcccgacc agacaccgat
     1201 gcggttatca tactctttga gaaggttgaa gttcatggct cccgatccga tggactcgtc
     1261 gaggatgatc tgaccgtgct cctgtgatgg cagtgacagg aggaacagcg ggtctttacc
     1321 ggtgccctgc agcactccgt cgaagcgaaa ggtccatacg taatcgggcc gggcgggggt
     1381 gagcggataa cccagcgaga cgatgcggta ggtgacaatg ataagagata tggcaatgag
     1441 cgccagcgct gccgccaggg ccggtcgttt catctgtcga cctctgtatc ctctgtttcc
     1501 ggagaattcc agaaggattg cgaggtatca acgaggaaat tgcctgtgat cacgtttctg
     1561 ccgatgagca aaggatatct catgcgcgag cggtcgttga gattgacccg ggcccgcagc
     1621 tgctttgagg cgatgcacag ttccagttcg atgaccggcc gcttctcgcg cgacttggag
     1681 gtgcggacgt acttccagtc gacgatacgg gccttgatgg tggcgcctcc ccatttggga
     1741 ggaagagcga aggtgacggt cgactcctcc acgcgtatgt tcctggcgtc cagggatgtt
     1801 gttgcggctc ccgtatcgat gcgcgccggg accgtgaacc cgtagggcag tatcctgaca
     1861 tattcttcgc ctcctatgac gggtttatcc ggctcgccca gggtatggtc gggaaaggcg
     1921 caggtgagga acaacagtgc cgggatgatg aagcaggagg cgtggttgct tatttcccgg
     1981 aggatgtcct tggctgtcac agcatgtatt atagcacata gccttcgcgg gatagacaaa
     2041 aaagccgcgc tcccggcggt cacatatgat ataatggact ggtacgggtc gaaagatgac
     2101 aacggtacgc ttaccttcag tgagcgggat gttctacccc gaagatcccg tccttcttag
     2161 aaaggatatc gaagcctacc tgaaaggggc ggaggttgag cccatctccg gtcgggtcca
     2221 cgcgatagtc tctccccatg cggggtatgt ctattcgggg caggtggcgg cctttgcgta
     2281 caaggcggtg tccgggcttg aggtcgacag tgtcatcgtc atcgccccga gccacagggc
     2341 gtattttgaa ggcattgcct tatgggagac gggcagcttc agaacgccgc tgggggacat
     2401 aaggatcgac gagaaaggcg ccggggaact ccttgaaacg agggatgtct ttacggccaa
     2461 caggaatgtc cacaaagcgg agcactccct g
//
LOCUS       DAOQTR010000345         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_51237_length_2486_cov_3.896339, whole genome shotgun sequence.
ACCESSION   DAOQTR010000345 DAOQTR010000000
VERSION     DAOQTR010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51237_length_2486_cov_3.896339"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKN85_08350"
     CDS             <1..386
                     /locus_tag="PKN85_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44459.1"
                     /translation="DARVLEYVDEEISVGDYVTSGGEFPALVVIDAVCRFIPGVLGND
                     ESVSAESFREPLLEYPQYTRPETFMGSSVPPVLLSGNHEEIRKWRRKEAIKKTVLRRS
                     DLLQGFEPTEEDRRFMREIMEEFPE"
     gene            394..951
                     /locus_tag="PKN85_08355"
     CDS             394..951
                     /locus_tag="PKN85_08355"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNT44460.1"
                     /translation="MGVIHYPVYDKRGDVVATSLTNLEIHDMARTCMTFGLDMCYIVS
                     PLARQREIAERLIHHWIHGYGATYNPARGEALKKVRIRSDIGAMIGDFGSGPRPLVIG
                     TSSRKREGKTMSSGEFRDLVMMGRQPALVLFGTGWGLTEEVVELCDRMLEPISGAGDY
                     NHLSLRVALGIILDRIFSERGGHHE"
     gene            944..1291
                     /gene="rplS"
                     /locus_tag="PKN85_08360"
     CDS             944..1291
                     /gene="rplS"
                     /locus_tag="PKN85_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNT44461.1"
                     /translation="MNEMVDLLEKEHMRLDLPEVGIGDNVKVYTRIFEGEKERIQVFE
                     GVVIRKRGGNTRASFTVRKVSYGVGVEKTFPMHSPLIDRIERTTRSKIRRSKLYYLRN
                     LRGKAAKLKEKRD"
     gene            1302..1886
                     /locus_tag="PKN85_08365"
     CDS             1302..1886
                     /locus_tag="PKN85_08365"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNT44462.1"
                     /translation="MICRLTGLVGGIDEAGRGPLAGPVVSACVIWERAPEDKNGVTDS
                     KLLTQKERVGLFSWIMDNALSVGIGMASREEIDEMNILKASLLSMERALLATGMEPDV
                     VLVDGNRGLRNYPNARPVVRGDRRCFFMASASIVAKVIRDRAMDAYHRIYPNYNFKQN
                     KGYPTREHKAAIREFGITPIHRVTFKGVREYCAG"
     gene            1876..2232
                     /locus_tag="PKN85_08370"
     CDS             1876..2232
                     /locus_tag="PKN85_08370"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNT44463.1"
                     /translation="MPDKLTTGREGEDRAAAVLIREGYRILDTNFRSPFGEIDIVAEE
                     SGCLVFVEVKRRRGRGFGTSFDAIDARKKTHIIRSAQVYLKKNRCVDRRIRFDVVGID
                     GDTVRVIRHAFGEEKG"
BASE COUNT          575 a          627 c          792 g          492 t
ORIGIN      
        1 tcgacgcgcg cgtgctcgag tatgtcgatg aggagatatc ggtgggggac tacgtcacat
       61 cgggcgggga gttcccggcg cttgtcgtga tcgatgccgt ctgccgtttc atccccgggg
      121 tcctgggaaa tgatgaatcg gtatcagcgg agagtttccg ggagcctctc ctggagtacc
      181 cgcagtacac gaggcctgaa acgttcatgg gttcctcggt gccgcccgtc ctcctgtcgg
      241 ggaaccatga ggagatacgg aagtggcgtc gcaaggaggc gataaagaaa acggtcctga
      301 ggcgttccga cctgctccag gggttcgagc ctaccgagga ggaccgcagg ttcatgagag
      361 agatcatgga ggagttccct gaataacgta agcatcgggg tcattcatta tcccgtttac
      421 gacaagcggg gagacgttgt ggcaacgagc ctcacgaacc tcgagataca tgatatggca
      481 agaacttgca tgacttttgg tctggacatg tgctatattg tgtcacctct cgccagacag
      541 cgggagatag cggaaaggct gatccatcac tggatacacg gatacggggc aacgtacaac
      601 cccgcacggg gagaggccct caagaaggtg aggatcagat cggacatcgg cgcgatgatc
      661 ggggatttcg gcagcgggcc aagacccctc gttatcggta catcctctcg caaaagggag
      721 ggtaagacga tgtcctccgg cgaattccgc gacctcgtca tgatgggacg tcagccggcc
      781 ctggtgcttt ttggaacggg ctggggcctg acggaggagg tcgtggagct ttgcgacaga
      841 atgcttgaac ccataagcgg cgcgggtgat tacaatcatc tttcccttcg cgtcgcgctg
      901 ggtattatat tggatagaat tttcagtgaa agaggaggac accatgaatg agatggtcga
      961 tctgcttgag aaagagcata tgagactcga cctcccggag gtcgggatag gcgataacgt
     1021 taaggtctac acgaggatct tcgagggaga gaaggaaagg atccaggtct tcgaaggcgt
     1081 tgtcatccgc aagagaggcg ggaacacacg ggcgtcattc acggtccgga aagtctccta
     1141 tggtgtcggc gtcgaaaaga cgtttcccat gcattcgccc ctcatcgaca ggatcgaaag
     1201 gaccacgagg agcaagatca ggagatcgaa actgtactat ctgcgcaacc tcagggggaa
     1261 agcggcgaag ctcaaagaga aaagggatta gcagaaggac gatgatctgc cggctgacag
     1321 gtctcgtggg tggcatcgat gaggccggac ggggcccgct ggccggcccg gtggtttcag
     1381 cctgcgtgat ctgggagaga gcgccggaag acaagaacgg tgtgaccgac tcgaagcttt
     1441 tgacacagaa agagcgagtc ggtctttttt catggataat ggacaacgcg ctcagtgtcg
     1501 gtataggcat ggcgagccgg gaagagatcg acgagatgaa tatcctgaag gccagcctgc
     1561 tttccatgga gcgtgccttg ctggcaacgg ggatggaacc cgatgtggtg ctcgtggacg
     1621 gcaaccgggg tctcaggaat taccccaatg cccggcccgt tgtgcgcggg gaccgcaggt
     1681 gtttcttcat ggcaagcgcc tccattgtcg ccaaggtcat ccgtgacagg gccatggacg
     1741 cgtaccaccg tatctatccg aactacaatt tcaagcagaa caagggctac ccgacgcggg
     1801 agcacaaggc cgcgatcagg gaatttggga taacacccat ccatcgcgtg accttcaagg
     1861 gggtgaggga gtactgtgcc ggataaactg acgacagggc gggaaggaga ggaccgagcg
     1921 gcggccgttc tgataaggga gggctaccgc atcctcgaca cgaacttccg aagccccttc
     1981 ggagagatcg acatcgtcgc cgaagagagc ggctgtcttg tctttgtcga ggtcaaaaga
     2041 cgcaggggcc gcggtttcgg cacgtctttc gatgccatag acgcccgaaa aaaaacgcac
     2101 atcatacgtt ccgcccaggt ctatctgaag aagaaccggt gcgtcgaccg gcgtatccgt
     2161 ttcgacgtcg tcggcatcga cggtgacacg gtccgcgtga tacgccatgc tttcggagaa
     2221 gagaaaggat gatagcaaca gagtggtttg ctccatacct tcctggaagc tcatccgtca
     2281 tcccggcgga gacctcttcg actttcttcc cgatcaattc atccatactg gttcaacagg
     2341 acttcctgga ttccggcctt cgccggaatg acggattgga actggttgtc ggctttcata
     2401 agcctggacc tctgaggtcg acatgatcga cgagaaggtc atggagcttc tggcggccgt
     2461 gagggatgga cggatctccg tcgagg
//
LOCUS       DAOQTR010000346         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_51464_length_2478_cov_3.955427, whole genome shotgun sequence.
ACCESSION   DAOQTR010000346 DAOQTR010000000
VERSION     DAOQTR010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51464_length_2478_cov_3.955427"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PKN85_08375"
     CDS             complement(<1..609)
                     /locus_tag="PKN85_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNT44464.1"
                     /translation="MAMFTPIKPLRISNEVTEQLKQSILLGHFKPGDKLPSERELAEE
                     FQVSRVAIREALRTLENAGFVVTRQGITGGAFVTDLSFQQLTNSFLDLFLCEKLSIPE
                     LYHVRRLIEPEVARLAALRINDEYARRLRTALEAEKTPGPTISEEVDRKTTVHYILAE
                     MCGNRFFEALVTCVMRLTKQVVEAVKPDTEKLHPAGLHDAAVE"
     gene            826..1077
                     /locus_tag="PKN85_08380"
     CDS             826..1077
                     /locus_tag="PKN85_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44465.1"
                     /translation="MNRRSSIGITAVVCLFVLVLLAGSVPSYAQEKVITLNFSNFFPA
                     SHKNSIIMADWCKEVEKRTKGRVKITYYPGAVLTPAGQT"
     gene            1087..1854
                     /locus_tag="PKN85_08385"
     CDS             1087..1854
                     /locus_tag="PKN85_08385"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT44466.1"
                     /translation="MKGIADIGESVLGYTKGRFPLTEVIDLPLGYKNAYAATRMINAY
                     FKKFQPKEFDQVKVMYLHAHGPGILFTKRPVQNLEELKGMKVRSTGFSAKVAAALGAS
                     PVAMPQTESYEALRTGIVEGILNPIEAMKGWKIGEVVKYTIENYGSAYSTSFFVVMNK
                     EKWKALPPDIQKTIEGINEEWMEKQGRQWDAIDKEGREFMVQRGNKFIALSKAEDARW
                     AAKVRPVIDDYVKELKTKGLPGEEALKFCLEYLKANQ"
     gene            1973..>2478
                     /locus_tag="PKN85_08390"
     CDS             1973..>2478
                     /locus_tag="PKN85_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44467.1"
                     /translation="MAHQMNTYWNPILETMPREKLRELQLRKFKRIVEYAYENSPFYR
                     RLYSAAGMEPGDIKAFEDIAKVPKTDKGMLRKVQDNPPFPYGDILTVPLDEVTEFRQT
                     SGTTGTPVYQADTWQDWEWWSECWSYILYSQGYRSSDRVFLPFGYNIFVAFWAGHYAL
                     EKIGCEVVP"
BASE COUNT          590 a          620 c          755 g          513 t
ORIGIN      
        1 ttcgaccgcg gcgtcatgga gacccgcggg gtgaagtttc tccgtgtcgg gcttgacggc
       61 ctcgacgacc tgcttcgtga gcctcatgac acaggtgacg agggcctcga agaaccggtt
      121 cccgcacatt tcggcaagga tgtagtggac ggtggtcttt cggtcgacct cttcggaaat
      181 ggtcgggccg ggggtctttt ccgcctcgag ggcagtcctg aggcgccggg cgtattcgtc
      241 gtttatcctg agcgcggcaa gccgtgccac ctcgggttct atgagacggc ggacgtggta
      301 gagctcgggg atggataact tctcacagag gaaaaggtcg aggaaggaat tggtgagctg
      361 ctggaaagac aggtcggtaa cgaaggcgcc tccggtgata ccctgacggg tgactacaaa
      421 accggcattc tcgagggttc tcaaggcctc cctgatagcc acccggctca cctggaattc
      481 ctccgcgagc tcccgctcgg aaggcaactt gtcgccaggc ttgaaatggc cgaggaggat
      541 ggattgcttg agctgctccg tcacttcgtt tgagatccgt aaaggcttta tgggcgtgaa
      601 catggccatt gtgctggtga tatcatatgt caggtctgcg gcaattgcaa taactttttt
      661 gttgacaaaa agatttcata tggataaata ttagaccaat agataaggac cattgggggg
      721 gataacatcg ttcggatccc tggaaagaca aaacaaagcg gacgaaaccc atgtatgagg
      781 ctacacatcc cgttttgggg tacatcaaat aacagggggt tcgttatgaa caggaggagt
      841 agtatcggaa taacggccgt agtctgtctg ttcgtgcttg ttcttctcgc aggttccgtt
      901 ccatcgtatg ctcaggagaa ggtgatcacc cttaatttct ccaatttctt cccggcgtcg
      961 cataagaaca gtatcatcat ggcggactgg tgcaaagagg tggagaaaag gacgaagggc
     1021 agggtgaaga tcacctatta tcccggtgcc gttctgactc cggccggcca gacctaggac
     1081 agtgttgtga aagggatcgc cgatattggc gagagcgttc tcggttacac gaaaggcagg
     1141 ttccctctta cggaggtcat cgatctgccc ctcgggtaca agaatgccta tgccgcgaca
     1201 aggatgatca acgcctactt caaaaagttt cagccgaaag agttcgatca ggtaaaggtc
     1261 atgtacctcc atgcccatgg accgggcata ctcttcacga agaggcccgt ccagaacctc
     1321 gaggagttga aggggatgaa ggtccgttcc acgggattca gcgcgaaggt ggccgccgct
     1381 ctgggggcct ctcccgtcgc gatgccccag acggagtcct acgaggctct gcgcaccggg
     1441 atcgtcgagg gcatcctgaa ccccatagag gcgatgaagg gctggaagat cggcgaggtg
     1501 gtcaaataca cgatcgagaa ctacggttcc gcgtacagca catccttctt cgttgtcatg
     1561 aacaaggaga agtggaaagc cctgcctcct gacatccaga agaccataga gggcatcaat
     1621 gaagagtgga tggaaaagca gggcaggcaa tgggatgcca tagacaagga gggcagggaa
     1681 ttcatggttc agcgcggcaa caagttcatc gccctgtcga aggccgagga tgcgagatgg
     1741 gcggcgaagg tccgtcccgt catcgacgac tacgtcaaag agctcaagac gaagggcctc
     1801 cccggtgaag aggcattgaa gttctgcctg gagtacttga aggcaaacca gtaatcggca
     1861 gccccacgca ttgcgggccg ggactacccc ctcctgaatg ggtcatggca accggctcgc
     1921 agtgctttac aatgtgatcg gagttgaaga aaatcaggga ggttgcggcg atatggctca
     1981 tcagatgaac acatactgga atccgattct cgagacgatg ccgagagaaa agctgcgcga
     2041 gctccagctc aggaagttca agaggatagt ggagtacgcg tacgaaaact cgcctttcta
     2101 ccgaaggctt tacagtgccg cgggcatgga gcccggagac atcaaggcct tcgaggacat
     2161 cgcgaaggtc ccgaaaacgg acaaggggat gctcaggaag gtgcaggaca atcccccctt
     2221 tccctatggg gacatcctca ccgttcccct ggacgaggtg acggagttca ggcagacgag
     2281 cggtacgacg ggcactcccg tctaccaggc cgatacctgg caggactggg aatggtggtc
     2341 cgaatgctgg tcatacatac tctattctca gggatacagg tcgtcggaca gggtctttct
     2401 gcctttcggc tacaacatat tcgttgcttt ctgggcagga cattacgcgc tggagaagat
     2461 aggttgtgag gtcgtgcc
//
LOCUS       DAOQTR010000347         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_51480_length_2478_cov_2.768469, whole genome shotgun sequence.
ACCESSION   DAOQTR010000347 DAOQTR010000000
VERSION     DAOQTR010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51480_length_2478_cov_2.768469"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1418)
                     /locus_tag="PKN85_08395"
     CDS             complement(261..1418)
                     /locus_tag="PKN85_08395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT44468.1"
                     /translation="MSDRSLRYEIGPIRPPNEAYSLLVRFTRNCPWNKCLFCQVYKGK
                     RFEKRSLDEIRSDIDTIKAIYDDIRELSWKKGFGGKITDSLVEAVFDSHVINESYKSV
                     AVWMYFGCKNVFIQDANSLAMKADVFIDALRYLKETFPTIDRVTSYARSRTLARSVSP
                     ESLKRMREAGLTRLHVGLESGHDPLLTYVNKGTTREDHMLAGRKVKEAGIELSEYVVL
                     GLGGKKWWREHALDTADVLNRIDPDFIRFRTLKVLRTMPLHEKLETGDFLLPDEEDIL
                     REERLLIENLEGIGSYVKSDHILNLLEEIDGRLPSDKKKFLETIDRYFNLSGEQRLIF
                     RFGRRSGIYRYLDDLGDELACFRIRKSIREMEEKEPGSVEKAISLLLENYI"
     gene            1908..>2478
                     /locus_tag="PKN85_08400"
     CDS             1908..>2478
                     /locus_tag="PKN85_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44469.1"
                     /translation="MRNLHNRPHRPHDVVDPADIGAGIAGGQAHTGENEKWLRLMADS
                     LPLSIVYIDRDFRYRFVNKTYESWYGRTRDDIYGRGVEEVLGPHEWSLLREYVERSLK
                     GQTVVFEQDISDRAGVSRHLRSKVLPCTGPGSQIDGVCMLITDTTEERQVETALRRSE
                     DRYHALFENIPVGICINTPDGRIVSYNSVL"
BASE COUNT          547 a          687 c          657 g          587 t
ORIGIN      
        1 attgacgaga gacaaagccg aagcccaaaa gccccctttt tcatggcaac gacaggaaaa
       61 atactctcac ggaccatcgt caaatgtcaa taaacaaaaa taggttgcgg gttgccagaa
      121 aaagactgcg gaaggagatc ggttgtcaac gcaattattg accagtgcat cgcatccttg
      181 cgccttgcta tgctcttttg gtctttgacc cggaacccgt aacccgtaac agccttattc
      241 ctatgaaacg gtcttctggt ctagatgtaa ttttcgagga ggagggaaat ggccttctca
      301 acgctgccgg gttccttttc ttccatttcc cggatggatt ttctgatgcg aaaacaggcg
      361 agttcgtccc ccaggtcgtc aaggtatcga tatatgccgg aacgcctgcc gaaacggaat
      421 atgagccttt gttccccgga gaggttgaaa tagcggtcga tggtctcgag gaatttcttc
      481 ttgtccgagg gaagccttcc gtcgatctct tccaggaggt tcaggatgtg atcgctcttc
      541 acgtagctgc ctatcccctc gaggttctcg atgagcagcc tctcctcccg gagaatgtcc
      601 tcttcatcgg ggaggagaaa atcgcccgtc tcaagttttt cgtggagcgg catggtcctc
      661 agcaccttca gggtccggaa ccttatgaaa tcggggtcga tgcggttgag gacatcggcg
      721 gtatcgaggg catgctccct ccaccacttc tttcctccaa ggcccaggac cacgtattcc
      781 gagagttcga tcccggcctc cttgaccttt ctgcccgcaa gcatgtggtc ctcgcgggtc
      841 gtccccttgt tgacgtaggt gaggagagga tcgtgaccgg attccaggcc cacgtggagc
      901 cttgtcaatc cggcctccct cattctcttc aggctttccg gcgatacgct cctggccagc
      961 gtgcgggacc gcgcgtagga cgtaacgcgg tcgatggtgg gaaaggtctc cttcagatac
     1021 ctcagggcgt cgatgaaaac atcggccttc atggcaaggg agttcgcgtc ctgaatgaag
     1081 acgttcttgc acccgaaata catccagacg gcgaccgact tgtagctctc attgatgacg
     1141 tgactgtcga acaccgcttc cacgagggaa tctgtgatct ttcccccgaa accctttttc
     1201 cacgagagtt ccctgatgtc gtcgtagatg gccttgatgg tatctatgtc cgacctgatc
     1261 tcgtcaaggg agcgcttctc gaaacgcttc cccttgtaga cctggcagaa gaggcacttg
     1321 ttccagggac aattcctggt gaaccgcaca agaaggctgt aggcttcgtt gggtgggcgt
     1381 atgggcccta tctcgtatcg cagggacctg tcgctcattc cttgtctaca tgaacgggaa
     1441 atgccggggt agacgccttg gacatcgctt tcttcctctt ctggttctgg gatgctttct
     1501 tcctttttct ttttaattcc gtcttcttgg aaagagatgg catgtggcct ccttacagtt
     1561 ttcgtcaaca taccacacaa cccccaatgt attcaagaag gtaaagcagg ccataaagca
     1621 ggtcatgggt gattggtcat gggtaatggg gaaacacgtt cctgtcacct gtcacctgtc
     1681 acctatgacc tatgacctat gacctatgac gccattattt atccccaaat gcggccaaat
     1741 cgtgctatta taatggcata gtgcacatca agacataagg cccgataccc acatgtcagt
     1801 catcgtctcg ctgcactggt tcttccgcca ccttccatcg gcatcctgtc gggccctgct
     1861 tcgaccaggc gtgatgtgac cgttccgaga ggaggtgcac ggaaaacatg agaaaccttc
     1921 acaacagacc gcacaggccc cacgatgtcg tcgaccccgc ggatattgga gcaggcattg
     1981 ccggcggaca ggcccacacg ggtgagaacg agaaatggct ccgcctcatg gccgattccc
     2041 tgcccctgtc gatcgtgtac atcgacaggg acttccgcta ccgtttcgtc aataagacct
     2101 acgagtcatg gtatggcaga acacgtgacg acatatacgg gagaggggtc gaggaggttc
     2161 tgggtcctca tgagtggtct ctcctcaggg agtacgtcga aagatcgcta aaaggccaaa
     2221 cggtggtctt cgagcaagac atctccgaca gggccggcgt ttcgcgtcat ctcaggagca
     2281 aggtcctgcc ctgcacaggt ccggggtcgc agatagacgg cgtatgcatg ctcatcaccg
     2341 ataccacgga ggaaagacag gtcgaaaccg ccctgcgcag gagcgaagat cgctatcacg
     2401 cactttttga gaacatcccg gtggggatat gcatcaacac gcctgacggc aggatagtgt
     2461 cctacaactc ggtgctcc
//
LOCUS       DAOQTR010000348         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_51554_length_2475_cov_3.247521, whole genome shotgun sequence.
ACCESSION   DAOQTR010000348 DAOQTR010000000
VERSION     DAOQTR010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51554_length_2475_cov_3.247521"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKN85_08405"
     CDS             <1..967
                     /locus_tag="PKN85_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNT44470.1"
                     /translation="LEFNHMAQDLTGYSRDDVIGRSHFEIIHGAQDPGSCPLFARVLK
                     EHTSSVASETLLKTRSGETVALLVTAFPLFNASGDFIGGVEFFRDISDKKRMERERRN
                     LFSMFAHDMKSPLAGVSGLLQRLFSGKAGPLSDKQRDHLSTIMRSISRLQAMVSKFVE
                     FSRLDTGKCDPVPGPYDINAALREQIEMVRLTAEKKGVQIVFEDAQVDVPAVEADSAM
                     IDRVLANLLDNAVKYTNAGGTVTVRLTSDARRVFVEVMDTGIGIREQDMPCVFDAFCR
                     VDRDSEGSGLGLSIARGIIEAHNGTLSVESVPGKGSRFWFVIPRR"
     gene            complement(998..2182)
                     /locus_tag="PKN85_08410"
     CDS             complement(998..2182)
                     /locus_tag="PKN85_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNT44471.1"
                     /translation="MMPLDTYEFNDVIHRCFRCGYCKFPTNWMDVNNCPPYARFRMES
                     YSCGGRLWLTRAWLNETLEWSEHLAEILYSCTTCRNCEVKCPLSFNVDIVNMVVAARS
                     EMIERGKVPPAVRRFLENIDLHGNPYGSARSRRDSWMQGTDIERYEGQEYLYYVGCTG
                     SYDTRAQRSARALATLLGKAGASFGVLGNHENCDGNEVHKLGEAGLFEMLAQDNIERF
                     KALGAKKIVTLSPHAYNAMKNLYPLYGGDFQVFHYTELLYELFKEGKLDVSNGFDARL
                     TYHDPCFLGRWNDGYETPRRLLAAVPSLRLAEMEKSRDAALCCGGGAGNFEIDLLGGG
                     DSSPARRRVRHAAETGATVLAVACPKCLVMLEDALKAEELEEKLAVRDIAEILTEAGG
                     IR"
     gene            complement(2179..>2475)
                     /locus_tag="PKN85_08415"
     CDS             complement(2179..>2475)
                     /locus_tag="PKN85_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT44472.1"
                     /translation="DIPYTVTGRCVGVGHSMMFAWTYAFNRADPGTMREAREALHETD
                     DLVLELGGVIWKPGTYGQQLILGRLDPGTRALMRRIKEVLDPGGIMNPGNWEGI"
BASE COUNT          462 a          732 c          750 g          531 t
ORIGIN      
        1 cctggagttc aatcacatgg cgcaggacct cacgggttat tcccgggacg acgttatcgg
       61 cagatcacat ttcgagatca tccacggcgc gcaagacccc ggttcgtgtc ccctgtttgc
      121 gcgtgtgctc aaggagcata cctcttccgt cgcgtctgag acattgttga agacgaggag
      181 cggcgaaacg gtcgcgctgc tcgtcacggc ctttccgctt ttcaacgcct cgggagactt
      241 cataggggga gtcgagtttt tcagggacat ctcggacaaa aagcgcatgg agcgggaacg
      301 caggaacctc ttctccatgt tcgctcacga catgaagagt ccccttgcag gggtttccgg
      361 actattgcag agactgttct ccgggaaggc gggccctttg agcgacaagc aaagggatca
      421 cctttccacc attatgagat ccatttcgag actgcaggcc atggtgtcca aattcgttga
      481 attttcaaga ctggacaccg gcaagtgcga tcccgtccct ggcccgtacg atatcaatgc
      541 ggccctcaga gaacagatcg agatggtgag gctgacagct gagaagaaag gcgtgcagat
      601 cgtttttgaa gacgctcagg ttgacgtgcc ggcagtggaa gccgatagcg cgatgataga
      661 cagggttctg gccaatctcc tggacaacgc cgtgaaatac accaatgcgg gggggacggt
      721 gacggtacgc ctcacgagcg acgcgcgccg tgtttttgtc gaggtcatgg ataccgggat
      781 cggtatccgc gagcaggaca tgccctgtgt attcgatgct ttttgccgtg tggaccgtga
      841 cagtgaaggt tccggtctgg gcctgtcaat agccaggggg atcatagaag cccacaatgg
      901 aacgctgtcg gtcgagagtg tcccgggaaa gggcagcaga ttctggtttg tcattcccag
      961 gcggtgaagg acgggaagac ggacagcgcg ggccccatca tcgtattccg ccggcctctg
     1021 tcagtatctc cgcgatgtcc cttacggcca gcttctcctc gagttcctcc gccttgaggg
     1081 cgtcttcgag catgacgagg catttcggac aagccaccgc gaggacggtc gctcccgttt
     1141 cggcagcgtg tctgacccgg cgtcgcgccg ggctcgaatc gccgccgccg agaaggtcta
     1201 tctcaaaatt gccggcccct ccgccgcagc acagtgccgc gtccctgctc ttctccatct
     1261 cggccaaccg aagggaggga acagcggcga gaagcctcct cggtgtctcg tacccgtcgt
     1321 tccaccgccc caggaagcac gggtcatggt atgtcagccg cgcgtcgaat ccgtttgaaa
     1381 cgtccagttt tccttccttg aagagttcat agagcaattc ggtgtagtgg aatacctgga
     1441 agtcgccgcc gtacaggggg tagaggttct tcatcgcgtt gtaggcgtgg ggtgaaaggg
     1501 tgacgatctt cttcgctccg agggccttga agcgctcgat gttgtcctgc gccagcattt
     1561 cgaagaggcc tgcctcgccc agcttgtgca cctcgttgcc gtcgcagttc tcatggttgc
     1621 ccagcactcc gaaggaagcg ccggccttgc cgagcagggt cgcgagggcc cgggcggacc
     1681 tctgcgcccg cgtgtcgtag gagcccgtgc atccaacgta gtacaggtac tcctggccct
     1741 cgtatcgctc tatgtccgtc ccctgcatcc acgagtcgcg cctgctgcgg gcgctgccat
     1801 aagggttgcc gtggaggtcg atgttttcga ggaatctcct cacggctggc gggacctttc
     1861 cccgttctat catctcgctc ctcgccgcca ccaccatgtt gacaatgtcc acgttgaagc
     1921 tcaatggaca cttgacctcg cagttcctgc aggtggtgca ggagtagagg atctcggcaa
     1981 ggtgttccga ccactcgagt gtctcattca gccacgcccg tgtcagccag agccttccgc
     2041 cgcaggaata ggattccatg cggaagcggg cgtagggtgg gcagttgttc acgtccatcc
     2101 agttcgtggg gaacttgcaa tagccgcatc tgaaacagcg gtggatgaca tcgttgaatt
     2161 cgtaggtgtc taaaggcatc atattccctc ccagtttccc gggttcatga tgcctccggg
     2221 gtcgaggacc tctttgatgc gtctcatgag cgcccgcgtg cccgggtcga ggcgcccgag
     2281 gatgagctgt tgtccgtacg tccccggctt ccatatcacg ccgcccagtt ccaggacaag
     2341 gtcatcggtt tcgtgcaggg cctcccgtgc ctcacgcatc gtcccggggt cggcccggtt
     2401 aaaggcgtag gtccacgcga acatcatcga gtgcccgacg ccgacgcacc ggcccgtcac
     2461 cgtgtagggg atgtc
//
LOCUS       DAOQTR010000349         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_51585_length_2474_cov_3.020256, whole genome shotgun sequence.
ACCESSION   DAOQTR010000349 DAOQTR010000000
VERSION     DAOQTR010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51585_length_2474_cov_3.020256"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKN85_08420"
     CDS             complement(<1..265)
                     /locus_tag="PKN85_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44473.1"
                     /translation="MARNVMQVKEKKDRYQVAYIPGDDSGFDVMEAAMIVMQALDAPI
                     DWVRADMGWCMWEKTCKEFPEGDKRRNTVPQETIDKVRDTDATF"
     gene            complement(636..1127)
                     /locus_tag="PKN85_08425"
     CDS             complement(636..1127)
                     /locus_tag="PKN85_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNT44474.1"
                     /translation="MKITGKVWKYGDDVNTDVIFPGKYTYTVSDRAEMGKYALEDLDP
                     EFTRNAQTGDIIVGGKNWGCGSSREQAVTCLKVRGIGAIIAKGFARIYYRNALNEGLP
                     IVVSPEAVDALEKGQEVTIDFDRSEIVTPKGTFKFPPYPDFVKGLIEDGGLIPHVKKS
                     LGL"
     gene            complement(1132..2388)
                     /locus_tag="PKN85_08430"
     CDS             complement(1132..2388)
                     /locus_tag="PKN85_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNT44475.1"
                     /translation="MGKTFAEKVLGKAAGESVSAGQVVIVEPHFCMSHDNAAPIWGTF
                     KKIGVDKVWKPDHLVFILDHAIPAPTDKHAENHKQIREVVKEQGIRYFYDVTSKGGVC
                     HQIMCEEGFALPGLVMVGSDSHTCTYGAFGAFSTGIGRSEMAATWATGQIWFRVPESM
                     KITVKGKFNKGVSAKDLILKIAGDIKADGADYMSIEFHGPAIEEMSLAERMTLCNMGI
                     EIGAKNAVCPPDQKVLDFIKPIAKTDEWEAMWADEDAVYAQELNYDLGDIEPCVAKPH
                     TVDNYAPIGEVKGTPVHQAFLGSCTNARIEDLRLAADILKDKEVAVRTIVIPASWSVF
                     RQAMKEGLIDVFLDAGCVITNPGCGPCMGNHEGILAPGETCISTANRNFKGRMGNKES
                     NIYLASPMTVAASALKGEISDPREVL"
BASE COUNT          417 a          747 c          675 g          635 t
ORIGIN      
        1 tgaaggtggc gtcggtgtcc ctgaccttgt cgatggtctc ctggggtacg gtgttccttc
       61 tcttgtcacc ctcggggaac tccttgcagg tcttttccca catgcaccag cccatgtcgg
      121 cgcgaaccca gtcgatgggc gcgtcaaggg cctgcatgac tatcattgcg gcttccataa
      181 cgtcgaagcc cgaatcatcc cccggaatat aggctacctg gtatctgtcc ttcttctcct
      241 tgacttgcat aacgttcctg gccatattat tgcctccttg ttttaaaaag cgtaacttgt
      301 aactcgttac ttgttactcg tgaatcgtga accgggaggg gcaaaggcag ggccccgtcc
      361 accgttagcg cccttgatcc agccccctgc ctctcagatt ttgactctcc cacaaaaaga
      421 cccgggtttt cctctctctt gcacccctct gcggccctct gtgacctctg tggtgagatg
      481 gtttgatttt ggctttggat cgttccctcc cgttcaccgt taaccgttta cgcacttgat
      541 cctggaagtc acgagtcatg cgttacggtc ttgcagcctt gctttccgtt atccgagtta
      601 cgagttacgc gttacgagtc acgcgttacg gtcttttaaa gccccagact cttcttcacg
      661 tgggggatca gccccccgtc ctcgatcaga cccttcacga aatccgggta gggcgggaac
      721 ttgaaggtcc ctttgggtgt cacgatctcg ctcctgtcaa aatcgatggt gacttcctga
      781 cccttctcaa gcgcgtccac cgcttccggg cttaccacga tgggcagccc ttcgttgagg
      841 gcgttgcggt agtagatcct ggcgaaaccc tttgctatga tcgcgccgat gccccttacc
      901 ttgaggcatg tgacggcctg ctcacgggaa gagccgcagc cccagttctt tccacccacg
      961 atgatgtccc ccgtctgtgc gttcctggtg aactcggggt cgagatcctc gagggcgtac
     1021 ttgcccatct cggcgcggtc agagacggtg taggtgtact tgcccgggaa aatgacgtcg
     1081 gtgttgacgt cgtccccgta tttccagacc tttccggtga tcttcatggt cctaaagcac
     1141 ctccctggga tcggagatct cgcccttgag agcggaggca gcgactgtca tcgggctggc
     1201 aaggtagatg ttgctctcct tgttgcccat acgtcccttg aagttcctgt tggctgtcga
     1261 aatgcatgtc tcgcccggtg ccaggatccc ttcgtggttg cccatgcagg ggccgcagcc
     1321 cgggttggtg attacgcacc cggcgtccag gaagacatcg ataagccctt ccttcatggc
     1381 ctggcggaag accgaccatg aagcgggaat aacgatcgtc cttaccgcaa cttccttgtc
     1441 cttcaggata tccgcggcaa gccgcaggtc ctcgatgcgg gcgttggtgc agcttcccag
     1501 gaacgcctgg tgaacgggtg tgcctttcac ttccccgatg ggggcatagt tgtcgacggt
     1561 atggggtttg gcaacacatg gctcgatgtc gcccaggtcg tagttgagct cctgtgcgta
     1621 tacggcgtct tcgtcggccc acatggcctc ccattcgtcc gtcttggcga tgggtttgat
     1681 gaaatccagg accttctggt caggggggca tacggcgttc ttcgcgccga tctcgatccc
     1741 catgttgcag agggtcatcc tctccgccag agacatctct tcgatggccg ggccgtggaa
     1801 ctcgatggac atgtagtcag cgccgtcggc ctttatatcg ccagcgatct tgagtatcag
     1861 gtccttggcg gatactcctt tgttgaactt gcccttgacg gtgatcttca tcgactccgg
     1921 gacgcggaac catatctgac ccgtagccca ggtggctgcc atctcgcttc ggcctatacc
     1981 cgtggagaaa gctccgaagg cgccgtaggt gcaggtgtgg ctgtcgcttc ccaccatgac
     2041 cagaccgggc agtgcaaagc cctcttcgca catgatctgg tggcagacgc cgcccttgct
     2101 tgtgacatcg tagaagtacc tgatgccctg ttccttgacc acttcccgga tctgcttgtg
     2161 gttctccgcg tgcttgtctg ttggtgccgg aatggcgtgg tcaaggatga acaccaggtg
     2221 atcaggtttc cataccttgt caacgccgat cttcttgaag gttccccaga tcggtgccgc
     2281 gttgtcgtgg ctcatgcaga agtgaggttc gactatcacg acctgcccgg ccgaaacgga
     2341 ttccccagct gccttgccaa gaaccttttc agcgaatgtc tttcccatgc cgacctctcc
     2401 ctcctacttg ctggcggcct tttccttgag gtactcgatg aggccgccgg ccttcatgat
     2461 gtccatctcg aggt
//
LOCUS       DAOQTR010000350         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_51754_length_2469_cov_3.527341, whole genome shotgun sequence.
ACCESSION   DAOQTR010000350 DAOQTR010000000
VERSION     DAOQTR010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51754_length_2469_cov_3.527341"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1319)
                     /locus_tag="PKN85_08435"
     CDS             complement(276..1319)
                     /locus_tag="PKN85_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNT44476.1"
                     /translation="MANIAYLLVSLEGEAPESQRSLIGEYTSAKNLRIDNYITVELPS
                     RRRERLRRVKELLAGVNRWDVIVVSDLAALGDDIADVIAVVGDLLERGVRFIAAKQDM
                     DLNGVGDSSSKTATALFAMLTSVEREVASRRVREALAQKRKEGVVLGRPKGSLSASKL
                     DGKKELIIEYLSKGVSKASLARILETSPTNLASYLRTRNIAVSKKDPAMRRDASPKGS
                     PEREASPETPPPVAAPVQEVPAPQFHETTEVLLCRQCGKNIHDPRTITCAGGYVDYPN
                     GESHPRVPYPRHETERCPKCGVMPGGFHHEGCYMERCPKCGERLVSCGCRTRGEADEK
                     ASGDQSRSVRYLP"
     gene            complement(1590..2078)
                     /locus_tag="PKN85_08440"
     CDS             complement(1590..2078)
                     /locus_tag="PKN85_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNT44477.1"
                     /translation="MSYRVQVITVSDKGSRGDRVDTSGPAIKKMLEKDFIVGDVIIVP
                     DEADIIADAMKRAIDEEGMDMVVTTGGTGLSARDVTPEATRSVIERDLPGFAEVMRLE
                     SYRITPRAIISRAVCGIRRKSIIINLPGSEKAAVECLGFVIGAIPHALAKLKGDAADC
                     GA"
     gene            complement(2075..>2469)
                     /locus_tag="PKN85_08445"
     CDS             complement(2075..>2469)
                     /locus_tag="PKN85_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNT44478.1"
                     /translation="KKDHSHSPTDLLKDKGLMGDAHAGFMHRQVSLLARESIDKIRSK
                     GLDVNAGDFAENLTTEGIVLHELPVGTSLKTTGGVLLRVTQIGKECHTRCNIFQTVGD
                     CVMPREGIFAEVLTEGDVSVGDELEVIA"
BASE COUNT          482 a          764 c          679 g          544 t
ORIGIN      
        1 cgtcatatac cgcatccttc cctcataatt tcacggcaga ctacacgata ccacaatcgg
       61 gggtgttttg cacgccctct tcgcagacgg ggtcctgcgc cccaggtcat ccgcaatggc
      121 gagcgcgccg taaggaagat aatgaggttc ctggggatcc gccaggccag aagaacaaga
      181 atgtcctcag aaaaggcctc ttttcccgcg gcatttcaag acgtcattcc ttgatccacg
      241 tgaaaaaccg gctttcgaag gatttcagac agatctcagg gcaggtaccg cacagaccgg
      301 gattgatcgc ccgacgcctt ttcgtccgcc tcgccccggg tcctgcaccc gcacgagacg
      361 agcctttccc cgcatttggg gcagcgttcc atgtagcatc cctcgtgatg gaaccctccc
      421 ggcatgaccc cgcacttggg acagcgttcc gtttcgtgcc tgggataggg aacgcgggga
      481 tgggattccc cgttgggata gtcgacatat cccccggcgc aggtgatcgt tctgggatcg
      541 tggatgttct tgccgcactg cctgcacagg agaacctccg tggtctcgtg aaactgggga
      601 gccggcacct cttgcaccgg tgccgcgacg ggcggcggtg tctccgggga tgcctctctt
      661 tcgggggacc ccttcggaga ggcatccctc ctcatcgcgg gatccttctt tgataccgct
      721 atgtttcgcg tcctgagata gctggcgagg ttggtgggcg acgtttcgag gatgcgcgcc
      781 agagaggcct tgctcacacc cttcgacagg tattctatga taagctcttt cttgccgtcc
      841 agtttcgacg cggagaggct ccctttgggt ctgccgagga ccacgccttc tttcctcttt
      901 tgcgcgaggg cttctctgac acgcctggag gcaacctccc tctccaccga ggtgagcatg
      961 gcgaacaggg cggtggcggt ctttgaggag ctgtccccga caccattgag gtccatatcc
     1021 tgcttcgccg cgatgaaccg cacccctctc tccaggagat cgcccaccac ggcaatgaca
     1081 tcggcaatgt catctcccag tgccgcaagg tccgatacga cgatgacatc ccaccggtta
     1141 acgccggcaa ggagctcctt cacccgccga agacgctccc ttctccggga agggagctct
     1201 actgtgatat agttgtctat ccggaggttc ttcgcggagg tatattcccc tatgaggctc
     1261 ctctgagatt ccggggcctc accctcgagg gacacaagca gatacgctat gtttgccatt
     1321 atccatccct tgtcgaaaga ccttcgtcga ctcgccgaca acgagcgccg aaccgcaggt
     1381 catgatcatg cctgtttgca gtttatgaaa tgatcaaact cgccatatcg tatgaccata
     1441 atgtccaaca gaatcatccg ggcataatac agcacttatt tatatcacac tattatgccg
     1501 ggaatgaaat tgtaacacac tttcacccac agattacctg cagattagaa aaaattcaag
     1561 ggccaggcgc gtgccggccc ggaaagtatc taggccccgc agtcggccgc gtcaccctta
     1621 agcttggcca gggcatgggg gatggcgccg ataacgaagc cgaggcattc cacggccgcc
     1681 ttttcgctgc cgggaagatt gatgatgatg ctcttgcggc ggatcccgca aacggcgcgg
     1741 gagatgatgg cgcggggggt gatcctgtag ctctccaacc gcatgacctc cgcgaaaccg
     1801 ggaagatcac gttcgatgac cgaacgggtc gcctcggggg taacgtcgcg cgcggacaag
     1861 cccgtccctc ccgtcgtgac gaccatgtcc atgccttcct catcgatggc ccttttcatc
     1921 gcgtcggcta tgatatcggc ctcatcgggc acaatgatga catcacccac gatgaagtct
     1981 ttctcgagca tcttcttgat ggccggaccg ctcgtgtcga cacggtctcc acgtgagcct
     2041 ttgtcgctca ccgtgatcac ctgcacgcgg tagctcatgc aatgacctcc agttcatctc
     2101 ccaccgagac atcgccttcc gtgagcacct cggcaaagat cccctcccgg ggcatgacgc
     2161 agtcaccgac ggtctgaaat atgttgcagc gcgtgtgaca ttccttgcct atctgggtca
     2221 ctcgcaggag aacgccgccc gttgtcttca ggctcgttcc cacgggcagc tcgtgaagga
     2281 cgatcccttc cgtcgtgaga ttctcggcga aatcgccggc attgacgtcg aggccctttg
     2341 accggatctt gtcgatgctc tccctggcga gaaggctcac ctggcggtgc atgaaacccg
     2401 cgtgggcgtc acccataagg cccttgtcct tgaggagatc tgtcggggag tgagaatggt
     2461 cttttttca
//
LOCUS       DAOQTR010000351         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_51914_length_2465_cov_4.079668, whole genome shotgun sequence.
ACCESSION   DAOQTR010000351 DAOQTR010000000
VERSION     DAOQTR010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51914_length_2465_cov_4.079668"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="PKN85_08450"
     CDS             <1..1491
                     /locus_tag="PKN85_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNT44479.1"
                     /translation="VGPEDSIEAVKEKIIGTNQRFLPVVEKNELKGAITRTDLLRVLE
                     DEIAKTVLEKLEFHEKYVQRKNVRKLMEERLDEATMGKLVDMGNLADEMGFHAYLVGG
                     FVRDLLLRIENYDIDIVIEGDGIAFAEEMVRKFHTRMRSHREFATAKVLFPDGFKIDI
                     ATARLEYYRAPAALPTVEHGSLKLDLHRRDFTINTLAISLNRNTFGELVDFFSAQRDI
                     KEKTIRVLHSLSFVEDPTRVFRAIRFEKRFGFKIGKQTLNLIKNAVKLNFLARMRGKR
                     IWTELALILREDAPEAILARLAELDLLRFISPDIVFNKDKEKLFAQMHGVYQWHEFLY
                     QGKTVDKVQFYILAIVDNMKLSDIAEFAARMEISERFRRHTMENVERLRLAMARFSQG
                     VAGIKKSEVYRLLDGLSREALLFIMARTRSQEVKKAISNYITHHDSFRPFTTGNDLKR
                     LGVPEGPLYKEILEDLKDAKIDMDLKTKEAESHYLETYLGERGILT"
     gene            1488..2195
                     /locus_tag="PKN85_08455"
     CDS             1488..2195
                     /locus_tag="PKN85_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNT44480.1"
                     /translation="MSLLVPALEVQLECYEGPMAVLITLIKRNKVSIWDIPIALITDR
                     FLQYVEIAKEMNLKIAEDFIEMASLLLFIKSKMLLPSNGATDEEDPREELVERIIEYE
                     RVRNMAVAIDDLPMLERDVYCIGRGSLEKETDFDLLCLCNLYFELMNVKEEPYFMVRD
                     VRPTLEEKIAALKDLLRSDGFFEWDLVAEEERNERVATILGMLELAKIRVATLTQRRP
                     FGRIIMKTRDAAALAEG"
     gene            2265..>2465
                     /locus_tag="PKN85_08460"
     CDS             2265..>2465
                     /locus_tag="PKN85_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44481.1"
                     /translation="MAGRTGRKVLLIVTVVIAAVIFLASILPGIIGGAGGGKIGVVEV
                     EGTIADLKDVMADIVRFKEDKGI"
BASE COUNT          575 a          675 c          736 g          479 t
ORIGIN      
        1 gtgggtcccg aggactccat cgaggcggtg aaggagaaga tcatcggcac caaccagcgg
       61 ttccttcccg tggtggagaa gaatgaactc aagggggcca tcacgaggac ggacctcctg
      121 agggtcctcg aggacgagat cgccaagacc gttctggaaa aactcgagtt ccacgaaaag
      181 tacgtgcagc gcaagaacgt ccgcaagctc atggaggagc gactggatga ggcgacaatg
      241 gggaagctcg tggacatggg caaccttgcc gacgagatgg gttttcatgc ctacctcgtg
      301 ggcggtttcg tccgcgatct ccttcttcgc atcgagaact acgacatcga tatcgtcatt
      361 gagggtgacg gcatcgcctt cgccgaggag atggtcagga agttccacac caggatgcgt
      421 tcccacaggg agttcgcgac ggcaaaggtg ctctttcccg acgggttcaa gatcgacatc
      481 gcgacggccc gcctcgagta ctacagggca ccggccgcgc tgcccaccgt cgagcacggc
      541 tcgctgaaac tggacctgca ccgccgcgat ttcacgatca acacccttgc catatccctt
      601 aacaggaaca ccttcggcga actcgtggac ttcttcagtg cccagcggga catcaaggaa
      661 aagactatcc gtgtcctcca cagcctgagc ttcgtggagg accctacgag agtgttccgc
      721 gccatccgct ttgagaagcg attcgggttc aagatcggca agcagaccct gaacctcatc
      781 aagaatgccg taaagctgaa ttttctcgct aggatgcgtg gaaagagaat atggacggaa
      841 ctggccctta tcctgaggga ggatgccccc gaggccatcc tcgccaggct cgcggaactc
      901 gaccttctgc gtttcatctc gccggacatc gtcttcaaca aggacaagga gaagctcttc
      961 gcgcagatgc acggcgtata tcaatggcac gagttcctct accaggggaa gacagtcgac
     1021 aaggtccagt tctacatcct tgccattgtg gacaacatga agctctccga catcgcggag
     1081 ttcgcggcgc gcatggagat aagcgagcgt ttccggaggc acaccatgga gaacgtggaa
     1141 cgcctgcggc tggccatggc gcgtttttcc cagggagtgg cgggcataaa aaagagcgag
     1201 gtctatcgcc tgctggatgg actttcccgg gaggccctgc ttttcatcat ggccaggacc
     1261 cgttcccagg aggtaaagaa ggcgatctcg aactacatca cccatcacga ctcgttcagg
     1321 cccttcacga ccggcaacga cctcaagagg ctcggtgtgc ctgaaggacc tctttacaag
     1381 gagatcctcg aagacctcaa ggatgccaag atcgacatgg acctcaagac caaggaagcg
     1441 gagtcccact accttgagac ctatctcggc gaaaggggga tcctcacatg agtcttctcg
     1501 ttcccgcgct ggaagtccag cttgaatgct atgaggggcc catggcggtc ctcatcactc
     1561 ttatcaagcg gaacaaggtg agcatctggg acatccccat tgccctcatt acggaccgct
     1621 tcctccagta cgtcgagata gcgaaggaga tgaacctgaa gatagccgag gacttcatag
     1681 agatggcctc cctccttctc ttcatcaagt cgaagatgct ccttccttcc aacggggcga
     1741 cggacgagga ggacccccgc gaagaactcg tggagcgcat catcgagtat gaacgcgtca
     1801 ggaacatggc tgtcgcaatc gacgacttgc ccatgctgga acgcgacgtc tattgcatcg
     1861 ggcggggcag cctggaaaag gagaccgatt tcgatcttct gtgcctgtgc aatctctact
     1921 tcgagctcat gaacgtcaaa gaggaaccct atttcatggt gcgtgatgtg agaccgacgc
     1981 tggaggagaa gatcgcggcg ctcaaggacc ttctgaggtc cgatgggttc tttgaatggg
     2041 acctcgtcgc cgaggaagag aggaacgagc gcgtggccac cattctcgga atgctggaat
     2101 tggccaagat acgggtggcg acgctgacac aacggcgacc ttttggtaga attatcatga
     2161 agacacggga cgccgcggcg ctggccgaag gatgagtcgc ggtgccgatc tgacaggagc
     2221 agcgggagcg gttttctggc acgggcgctg caaggaggaa tgaaatggcc ggtcgcacag
     2281 gcaggaaggt gttgctcatt gttaccgtcg tgatagcggc tgtgatcttt ctcgcgagta
     2341 ttctgcccgg catcatcggc ggagccggcg gaggaaagat cggtgtcgta gaggtcgaag
     2401 gcacgatcgc tgaccttaag gacgtcatgg ccgatatagt caggttcaag gaggataaag
     2461 gtatc
//
LOCUS       DAOQTR010000352         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_52138_length_2457_cov_4.922565, whole genome shotgun sequence.
ACCESSION   DAOQTR010000352 DAOQTR010000000
VERSION     DAOQTR010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52138_length_2457_cov_4.922565"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKN85_08465"
     CDS             <1..339
                     /locus_tag="PKN85_08465"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44482.1"
                     /translation="GDVLGIEAARNAIINEATDTLREQGLSVDVRHIMLVADIMTCDG
                     EVKQIGRHGVSGEKASVLARAAFEVTVNHILDAAVRGDVDDLRGVTENVIVGQPIQLG
                     TGDVQLVAKR"
     gene            349..648
                     /locus_tag="PKN85_08470"
     CDS             349..648
                     /locus_tag="PKN85_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNT44483.1"
                     /translation="MINIDRALRSSIRTGKVVLGSNLALEAGTKGQAKLFISAIDCPE
                     EVAVELKKADVPVYSYSGRGKDLGSACGKALLGRGSGGDRARRIGDHGAPEGDQG"
     gene            654..1085
                     /locus_tag="PKN85_08475"
     CDS             654..1085
                     /locus_tag="PKN85_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HNT44484.1"
                     /translation="MSEFKLTTEGIRYIALFESLTGGVARDCIIDDENDRIIFVIKKG
                     DMGAAIGRKGSNINRVKKSIGKQIEIVEHSDDVKEFLENVFQPASIKNVTIVSKNNKR
                     LAYVEVANRDKGIAIGRDGRNILKAKMLSSRHHGVDDIIIQ"
     gene            1161..1589
                     /locus_tag="PKN85_08480"
     CDS             1161..1589
                     /locus_tag="PKN85_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNT44485.1"
                     /translation="MGKGMYAARKLESDRKSFRWKDLNYSRRVLKLNVKADPLGGAPR
                     ARGIVLEKVGVEAKQPNSAIRKCVRIQLIKNGRQVTAFCPGDGAIGFIDEHDEVVVER
                     IGGRMGGAKGDIPGVRFQVVAVNNVALNEMVSGRKEKPVR"
     gene            1594..2157
                     /locus_tag="PKN85_08485"
     CDS             1594..2157
                     /locus_tag="PKN85_08485"
                     /inference="COORDINATES: protein motif:HMM:NF003106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNT44486.1"
                     /translation="MSVKIFNKWDPEEIEVADQSIKRYFNLRPTVVMHSGGRHARQQF
                     KKSEQHIVERLINKMMQGEDNTGKKMKTYKIVEEAFDIVHQRTKENPLSVFAKAISNA
                     GPREEVVRLKYGGITVPKAVDTAPQRRVDVALNFISTGAQRSAFKSKRSAASCLADEI
                     IAASKGDVRSFAMNRKDSVERVAKAAR"
BASE COUNT          613 a          642 c          782 g          420 t
ORIGIN      
        1 ggcgatgtcc tggggatcga ggcggctcga aacgccataa taaacgaggc caccgacact
       61 ttgagagaac agggcctctc cgtcgatgtg aggcacatca tgctggtggc cgacatcatg
      121 acctgcgacg gcgaggtgaa gcagataggc cgccacggcg tctcggggga gaaggcgagc
      181 gtcctcgcgc gggccgcctt cgaggtgacg gtcaaccaca tcctcgacgc cgcggtccgg
      241 ggagacgtgg acgatctcag gggagtgact gagaacgtca ttgtaggcca gccgatccag
      301 ctgggaacgg gcgacgttca gttggtcgca aaacgatgag gtaaaaggat gataaatatt
      361 gatagagcgc tcagaagttc gatcagaacc ggtaaagttg ttctcggctc aaatctcgcc
      421 ctggaggccg ggaccaaagg gcaggcaaag ctcttcatca gcgccatcga ctgcccggaa
      481 gaggtggcag tcgagctgaa gaaggccgac gtccccgtct acagctattc gggaagggga
      541 aaggatctgg gctcagcctg cggaaaagcc cttctcggtc gcggctctgg cggtgataga
      601 gcccggcgaa tcggagatca tggcgctcct gagggagatc aggggtaacg aggatgagtg
      661 agttcaagct taccaccgaa gggatcaggt acatcgccct cttcgaaagc ttgaccggcg
      721 gcgtcgcgag agactgcatc atcgacgacg agaacgaccg gattatattc gtcatcaaga
      781 agggagacat gggagctgcc atcgggagga agggcagcaa catcaaccgg gttaagaagt
      841 cgataggaaa gcagatcgag atcgtggagc acagcgacga tgtcaaggag ttcctggaga
      901 acgtcttcca gcccgcctcg ataaagaacg tgacgatcgt ctcgaaaaac aataagcgat
      961 tggcttatgt agaagtagca aatagggaca agggcatcgc cataggccgg gacggccgca
     1021 acatattaaa ggccaagatg ctctcttcga ggcatcacgg cgttgatgac atcatcatcc
     1081 agtaaaaaaa gccggcgctc gactgaggtc ggatggggcc aacggttggc gatggacata
     1141 gcattcaact aggtgaaata atgggaaagg gaatgtacgc ggccaggaaa ctggagagcg
     1201 accgcaagag cttccggtgg aaagatctga actacagccg gagggtactg aagctgaacg
     1261 tcaaggcaga ccccctgggc ggcgcgccgc gggctcgagg catcgtcctg gagaaggtcg
     1321 gcgtcgaggc caaacagccg aactccgcca tcaggaagtg cgtccggata cagctgatca
     1381 agaacggaag acaggtcacc gccttctgtc cgggggacgg tgccatcggg ttcatagacg
     1441 agcacgacga ggtcgtggtg gagaggatcg gcggcaggat gggcggagcc aagggcgata
     1501 tcccaggcgt ccggttccag gtcgtcgccg tcaacaacgt ggccttgaac gagatggtct
     1561 cggggcggaa ggagaagccc gtaaggtgat accgtgagcg ttaagatctt caacaaatgg
     1621 gaccccgagg agatcgaggt ggcggaccag agcataaagc gctatttcaa cctccgtccg
     1681 acggtcgtca tgcattctgg cggcaggcat gcaagacagc agttcaagaa gtctgagcag
     1741 cacatcgtcg agcggctcat caacaagatg atgcaggggg aggacaacac cggcaagaag
     1801 atgaagacct acaagatcgt cgaagaggcc ttcgacatcg tccaccagag gacgaaggag
     1861 aaccccctct cggtctttgc gaaggcgatc tccaacgccg gccctcggga agaggtggtc
     1921 cgtctcaagt acggcgggat aaccgtcccc aaggccgtcg acaccgcccc ccagaggagg
     1981 gtggacgtcg ccctgaactt catctccacc ggagcccaga ggtcggcctt caagtccaag
     2041 cgttcggcgg ccagctgtct cgccgacgag atcatcgccg cctcgaaggg cgatgttcga
     2101 agcttcgcca tgaacaggaa ggacagcgta gagcgggtcg ccaaggcggc acgataaaac
     2161 ggggaacgaa gcgtttcgcc ttctgcgagc aaacgtttca aggccccgaa ccttattttc
     2221 gtttttgcgc tcgatgaaga tgagggcgcg cgtcccatcc gggtcgctcc cctcgccttc
     2281 aaccgagatt tttggcgtga tgtgatgatt tctaaagagt tggcccttta tccagtggtc
     2341 atccatcata aggtcttcga tgaaccgatc gcaatcgtga acctgtacga tagggtgctg
     2401 gtatgataga agaccatata gacgccccac ggctggctga cttccccagc actagat
//
LOCUS       DAOQTR010000353         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_52143_length_2457_cov_3.273938, whole genome shotgun sequence.
ACCESSION   DAOQTR010000353 DAOQTR010000000
VERSION     DAOQTR010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52143_length_2457_cov_3.273938"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..651)
                     /gene="coaD"
                     /locus_tag="PKN85_08490"
     CDS             complement(163..651)
                     /gene="coaD"
                     /locus_tag="PKN85_08490"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNT44487.1"
                     /translation="MKETLAVYPGSFDPVTFGHLDILARGLELFDKVIIAIAYNIEKQ
                     GLFTFEERKDLIQQSINGNSNVIIDSFDGLLIDYVKKVNARFVIRGLRAMSDFEYEFQ
                     MASINRTLNPGMDTLFMMTSKDYFFISSRTIKEVAAFHGSVSSFVPAPVERRLKEIFK
                     KR"
     gene            complement(661..1236)
                     /gene="rsmD"
                     /locus_tag="PKN85_08495"
     CDS             complement(661..1236)
                     /gene="rsmD"
                     /locus_tag="PKN85_08495"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNT44488.1"
                     /translation="MATLRITGGRLKGMSIGVLPGDVARYTSSKVREALFNMIGDVEG
                     MSVLDLFAGSGSFSIEALSRGAASATLVEANRKMTDLIGKNLARTGLNKYCQVLHMDV
                     RYAVPLLYGRKCMYDVIFMDPPYEMGYVTRTMTLLDEHPLRNSDTITIAEYSRRESDC
                     LSLFEGLKGARTRKYGDTMISLFRDPLNPGA"
     gene            complement(1242..2186)
                     /locus_tag="PKN85_08500"
     CDS             complement(1242..2186)
                     /locus_tag="PKN85_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNT44489.1"
                     /translation="MGLAGIDGSGVKLIMVGGKGGVGKTTCASAIALKMAEEGRKVLI
                     ITSDPAPSLSDIFEKEIGDRETRIDDALELYGLEVSSDIVLARWKERFGPEIYEVVSS
                     FASVDYDFVEYIGTAPGIEEEYMLNFIMELVEGGSYDVVVWDTAPAGHTLRLLRLPQL
                     FLSHMEAATKFYMNMYGYIEKVKDTIRLKESKKTLLQIIAGWEALSERIVEFIRDGSC
                     VKYLIVTIPEALGVKMTNRVIGELEENRLTVENIVINNVVREADCEFHRLRKTMQEHY
                     IEELRETYRGKTLTLLYLAAQEIKGMDRIAGVTRSLFG"
BASE COUNT          521 a          694 c          662 g          580 t
ORIGIN      
        1 cggcatccgc cttgccggcc ttctcaatac attcccccag gaagcccgcc acgggcgggt
       61 tgatggcgcc ctgtatgcgg accgtgtaca catcttttgc gtgggagagt ggtgcgaaaa
      121 gaacgactgc gccgataagc gcaataatga agcacagcct cttcatcgct ttttaaagat
      181 ctccttgagt cgcctttcga cgggagcggg cacgaaactg gagacggagc catggaatgc
      241 ggcgacttcc ttgatcgttc tcgaactgat gaagaaataa tccttgctgg tcatcatgaa
      301 gagggtatcc atcccgggat tgagggtcct gttgatggag gccatctgaa actcgtactc
      361 aaagtcactc atggcgcgca ggcccctgat gacgaaacgg gcattcacct ttttcacgta
      421 gtcgatgagc aggccatcga aactatcgat aatgacattg ctgttcccgt ttatggactg
      481 ctggatgagg tccttccgct cctcaaatgt gaagagcccc tgtttttcga tattgtaggc
      541 gatggcgatt atgaccttgt cgaaaagttc aagccccctc gcgaggatgt cgagatggcc
      601 gaaggtcacg gggtcgaatg aaccgggata cactgcgagc gtctctttca tggtttctcc
      661 ttaagcaccg ggatttagtg ggtccctgaa cagcgagatc atcgtgtcgc cgtatttcct
      721 cgtccttgcg cctttgagcc cctcgaagag ggacagacag tcgctttcgc gcctggagta
      781 ctcggctatg gtgattgtat cggaattccg gagtggatgt tcatcaagaa gtgtcatcgt
      841 gcgcgtcaca tatcccattt cgtaaggagg gtccatgaag atcacatcgt acatgcactt
      901 cctgccataa agcagaggga ctgcatatct tacatccata tgtaacactt ggcaatactt
      961 atttaatccc gtcctggcga ggttcttccc gataaggtcc gtcatcttcc tgttcgcctc
     1021 gacgagtgtc gccgatgccg caccccggct cagtgcctct atggagaagg agccggagcc
     1081 ggcgaaaaga tcgaggacgg acatgccctc cacgtcgccg atcatgttga aaagggcctc
     1141 tctcaccttg gaggacgtat accgcgccac atcccctggc aggacaccga tcgacatccc
     1201 cttgagcctt cctcccgtta tgcgcagcgt tgccatgggt cctacccgaa gagcgacctg
     1261 gtcacgcccg ctattctgtc catgcctttt atctcctggg cggcgaggta caggagggtc
     1321 agggtcttgc ccctgtacgt ctctctcagc tcttcgatat agtgttcctg catggtcttg
     1381 cggaggcggt ggaactcgca gtcggcctcc cgcaccacgt tgttgatgac gatattctca
     1441 acggtgagac ggttctcctc gagctcgccg atgacccggt tcgtcatctt cacgcccagg
     1501 gcctcgggga tggtgacgat gaggtacttc acgcatgaac cgtccctgat gaactccacg
     1561 atacgttccg aaagggcctc ccagcccgcg attatctgga ggagcgtctt cttcgattcc
     1621 ttgagtcgta tcgtgtcctt caccttctcg atgtaaccgt acatgttcat gtagaacttc
     1681 gtcgccgcct ccatgtgcga caggaagagc tggggcagcc tgaggagcct cagggtatgc
     1741 cccgcggggg ccgtgtccca cacgaccacg tcgtagctgc cgccttccac gagctccatg
     1801 atgaaattga gcatgtattc ctcttctatc cccggggcgg ttcctatgta ctcgacgaaa
     1861 tcgtaatcga cgctggcaaa ggaagagacg acctcgtaga tctccggtcc aaaacgctct
     1921 ttccagcgcg ccaggacgat atcggaggag acctccagac cgtagagttc gagagcatca
     1981 tctatccggg tctcccggtc accgatctct ttctcgaaga tatccgaaag ggagggggcc
     2041 ggatcgctcg tgatgataag gacctttcgc ccctcctcgg ccatcttcag agcgatcgcc
     2101 gacgcgcacg tcgtcttgcc cacgccaccc ttgccgccga ccatgatgag cttgaccccg
     2161 gatccatcga tgcctgcaag tcccatagac gaacatcata ccatctttga cgcgggaaag
     2221 acagggaaga cggacaggaa acaggtttta cgttttaggt tttacgtttt aggttttagg
     2281 caaaaacgtc ataatttctt tcacttaaat tcttcctcgt ggcccgtgaa catcgccttg
     2341 gtgtgggaga agggtgtcct gcccagggtt gccatgtaga ggtggacaat gagatagatc
     2401 aggaagacat atccaacgat cacgtggatg gcatcgagga cgcggatacc gcccgtg
//
LOCUS       DAOQTR010000354         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_52445_length_2448_cov_3.803176, whole genome shotgun sequence.
ACCESSION   DAOQTR010000354 DAOQTR010000000
VERSION     DAOQTR010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52445_length_2448_cov_3.803176"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..>2448
                     /locus_tag="PKN85_08505"
     CDS             189..>2448
                     /locus_tag="PKN85_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44490.1"
                     /translation="MRSGHPYRLSSRSYLLFILAFLFFLDGFALWLAITVDHDRTLEQ
                     AHAVLERTAVSLQERMERVVFSTEAILRNRASRIREQGMGRAVASRKEWEHFRRGAQS
                     LPGPGQLWLLDHRGNLVMSSTEYPSRRANYREKEYFSDQTDRAVDLYIGPITKDDIVK
                     NYSFTITKRIEGNDGTLLGVVVAGIETGIFADFLRRISPGEGGTVAVFRGDGALVLRQ
                     PMEEEYIGKGFSHLALFEQDFEASPWGSFRTDKAIDGVGRIIGYSKIVGLPLIAATTL
                     PIDSVLAGWRERVGIYSIVAIIAFMALVALSLVIRRTTSREEKERTRQLAEINASLQA
                     QIAERIRAQAALKESEKRWATTLASIGDGIIATDVAGRITFMNAVAEALTAWRSMDAP
                     GKPVGDVFTVIDEDTRQAIRDPVARVLEAGATVGLSGHTILIRRDGRELPIDDSGSPI
                     LDSEGNMTGVVLVFRDITDRRAAQKEITDSRRDLSRAQAVAHIGSWRLRVRERELTWS
                     DETYHIFGVIPGTPVTYEMFLAMVHPDDRETLIESWEGALAGAYYDVTHRIVVGGHIK
                     WVRAVAELEFDEEGTLLAGFGTVQDVTEQKRMEQALQVAHDELELRVEERTAEIRRQA
                     ELLDLANDAIIVTDTRGRMAFWNTGAVHTYGFTRKDMAGIMNHHTVLRTRFPIPVENI
                     LAIVESSGRWEGELTQTTKEGRRIVVLSRWAMRRGHSGRPPEIMEINTDITTRRQAEE
                     ALRLASVYNRSLI"
BASE COUNT          522 a          752 c          761 g          413 t
ORIGIN      
        1 tcaggggtat tgcctcagga gcacagaggt ggtaagtatg tccataccac gtgccgccga
       61 acgacgggtg acgggacaga caggacccgt cccttctctc ccccccgggt tctccccttc
      121 ccttgcacct tcgtgatgca tgtgcgataa ttaggcagca gtcgatcccc ccggtgagga
      181 acgcgatcat gagatcagga catccatacc ggctctcttc aagatcatac cttttgttca
      241 tccttgcctt cctcttcttc ctcgacggtt tcgctttgtg gctcgccata acggtggacc
      301 acgacaggac cctcgaacag gcccacgcgg tcctcgaaag aacggccgtc tcactgcagg
      361 aaagaatgga acgcgtcgtc ttctccacgg aggccatcct gaggaaccgt gcgtcgagga
      421 tccgcgagca aggcatgggg cgcgcggtcg cctcccggaa ggaatgggag catttccgaa
      481 ggggagccca atcgctgccc ggtccgggcc aactgtggct cctcgaccac cggggaaacc
      541 ttgtgatgag ctcgaccgaa tacccttctc gtcgggccaa ctaccgggag aaagagtatt
      601 tctcagacca gacggaccgg gcggtcgacc tttacatagg acccattacg aaggacgaca
      661 tcgtgaagaa ttactctttc accatcacca agcgcataga agggaatgac ggcacgctcc
      721 ttggtgttgt cgtggcaggc attgagacgg ggatctttgc cgacttcctt cgccgcatca
      781 gccccggaga gggagggacg gtcgcggtct tccgaggcga cggggccctg gtcctcaggc
      841 aaccgatgga ggaagagtac atcggcaagg gcttcagcca tctggccctt ttcgagcagg
      901 atttcgaagc gagcccctgg ggcagtttca ggaccgataa ggccatcgac ggggtcggtc
      961 gcatcattgg gtacagcaag atcgtgggcc tgcctctcat cgcggccacg acccttccca
     1021 tcgattccgt cctggccggg tggcgtgagc gcgtcgggat atattccatc gtggcgatca
     1081 tagccttcat ggcccttgtc gccctctccc tcgtgatacg caggaccaca tcgcgggagg
     1141 agaaggaaag gacgcgccag cttgccgaga tcaatgcgtc ccttcaggcc cagatcgccg
     1201 agcggatccg ggcacaggcg gccctcaagg aaagcgagaa gcgttgggcc acaacgctcg
     1261 ccagcatcgg ggacggcatc atcgcaaccg acgtggcggg caggataact ttcatgaacg
     1321 ccgtcgccga ggcgctgacc gcctggagga gcatggacgc cccgggcaaa cccgtgggcg
     1381 atgttttcac ggtgatcgat gaagacaccc ggcaggccat tcgtgatccc gtcgccaggg
     1441 tcctcgaggc cggtgcgacg gtgggtctct cggggcacac catactcatc cggagagacg
     1501 gcagggagct gcccatcgac gacagcgggt cccccatact cgactccgag ggcaacatga
     1561 cgggtgtcgt gctcgtcttc cgggacataa cggaccgccg ggccgcgcag aaggagatca
     1621 ccgactcgag acgcgacctc agcagggcac aggccgtggc ccacatcgga agctggcgcc
     1681 ttcgagtccg ggagcgtgaa ctgacgtggt ccgacgagac atatcacatc ttcggggtca
     1741 taccggggac ccccgttaca tacgagatgt tcctggccat ggtgcatccc gacgaccggg
     1801 aaacgcttat cgaaagctgg gaaggggcgc tcgcgggggc atactatgac gtcactcacc
     1861 gcatcgtcgt cgggggccac atcaagtggg tgcgcgcggt ggcggaactg gagttcgacg
     1921 aggaaggaac gctgctcgca gggttcggaa cggtccagga cgtgacggag cagaaacgga
     1981 tggagcaggc cctgcaagtg gcccacgacg agctggagct tcgcgtcgag gagaggaccg
     2041 ctgagataag acgacaggcg gaactcctcg acctcgcaaa cgatgccatc atcgttaccg
     2101 acacacgggg aagaatggcc ttctggaaca cgggggcggt ccacacatac ggcttcaccc
     2161 gcaaggacat ggccggtatt atgaaccacc acacggtcct ccgcaccagg ttccccattc
     2221 ccgtcgaaaa catacttgcc atcgtcgaaa gcagcggccg gtgggaaggc gaactgaccc
     2281 agacaacaaa ggagggacgg cggatagttg tcttaagccg ctgggcgatg cgccgcggac
     2341 actccggcag accccccgag atcatggaga tcaacaccga catcaccacc cgcaggcagg
     2401 ccgaagaggc ccttcgcctg gcaagcgtct acaacaggag cctcatcg
//
LOCUS       DAOQTR010000355         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_52530_length_2446_cov_2.354663, whole genome shotgun sequence.
ACCESSION   DAOQTR010000355 DAOQTR010000000
VERSION     DAOQTR010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52530_length_2446_cov_2.354663"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PKN85_08510"
     CDS             complement(<1..1127)
                     /locus_tag="PKN85_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT44491.1"
                     /translation="MSPSLDLPKNYEFSEVERRWQDAWRDEDNYFDPASTKPKFIIDT
                     PPPYPTGTFHIGNAFNWCYIDFIARYRRMRQFNVMFPQGWDCHGLPTEVKVEEIHGIT
                     KNDVPREEFRKMCRELTLSNIEKMRRTLRRMAFSVDWSHEYITMLPAYYGKTQLSFLR
                     MLAAGYIYQSEHPVNFCTRCETAIAFAEVAYEPRETTLNYFDFDGLEIATTRPELLAA
                     CVAVAVHPDDERYRGIPGRRLSVPLFGHEVPLITDPAVDPGFGSGAVMICTFGDKQDV
                     HWWKQHSLELRKAIDRQGRMTGIAGKYAGMKSSECRKAILDDMKGAQILTRQEVLEQR
                     VGTCWRCKTPIEIMSERQWFVKIDQDAIMKAAREITWNPEH"
     gene            1292..>2446
                     /locus_tag="PKN85_08515"
     CDS             1292..>2446
                     /locus_tag="PKN85_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNT44492.1"
                     /translation="MMNRLGERLAAVLVLSFILIAPVVQPPWLLAIMVVLFSMVLFLI
                     RKTRFLAMALIPIAILYGVSILPLLVFSCTLTIMVMGELAFRGTDERLYSYIGYIAAA
                     LVGCVLVMGYLNTWVPLVVLFGVVVAVLLRAILREREDALMIEALGIAMTMFLIYELN
                     YHADMALIGIAVAIAFSFAYFSYRAGTADVSGLFSAALIGIILIIFADIRWFLVVLAF
                     FIMGSASTKYRYDYKLRIGVEQVHGGARGYLNVFANGSVSAAAAVLWGVTGSPVFLAL
                     FVGSIATAAGDTMASEIGVTGGSPFLITTFERVPAGTNGGVTLTGEGVAIAGAFIVSL
                     LAFLLGIIDLPTAAACMVAGFIGTNIDSVVGALMENPGFVGNAGTNFLATL"
BASE COUNT          437 a          692 c          731 g          586 t
ORIGIN      
        1 atgtgctccg ggttccatgt gatctcgcgg gcagccttca tgatcgcgtc ctggtcgatc
       61 ttcacgaacc actggcgctc gctcatgatc tcgatcggag tcttgcacct ccagcaggtg
      121 ccgaccctct gctccaggac ttcctggcgg gtgaggatct gtgctccttt catgtcgtcg
      181 aggattgcct tgcggcattc ggaggatttc atgcccgcat attttcctgc gattcccgtc
      241 atccggccct gccggtctat ggctttccgg agttcgaggc tgtgctgctt ccaccagtgg
      301 acatcctgct tgtctccgaa cgtgcagatc atcacggccc cggacccgaa cccggggtcg
      361 acggcggggt cggtgatcag ggggacctcg tggccgaaga gaggaacgga gagccttcgt
      421 cccggaatgc cacggtaacg ttcgtcgtcc gggtggactg caaccgccac gcatgccgcg
      481 agcagttctg gtctcgttgt cgcgatctcc aggccgtcga agtcgaagta attgagggta
      541 gtctcccggg gttcgtatgc aacctcagca aacgcgatgg cggtctcgca ccgcgtgcag
      601 aagttgacgg ggtgctcgct ctggtagata taccctgcgg caagcattcg caggaaagag
      661 agctgcgtct ttccatagta ggccgggagc atcgtgatgt actcgtggct ccagtcgacg
      721 gagaaagcca tgcggcgcag tgtacggcgc atcttttcga tgttggagag ggtcagttcc
      781 ctgcacatct tcctgaactc ttcccttggg acatcgttct tggtgatgcc gtggatctcc
      841 tccactttca cctcggtcgg caggccatgg cagtcccatc cctggggaaa catcacgtta
      901 aactgcctca tcctccggta tctggcaatg aaatcgatat agcaccagtt gaaggcattt
      961 ccgatgtgaa acgtgccggt gggatagggg ggtggagtgt cgatgatgaa ctttggcttc
     1021 gtggatgccg ggtcaaaata gttgtcttca tcgcgccagg cgtcctgcca gcgccgttcc
     1081 acctccgaaa actcgtaatt cttggggaga tccaaagacg gcgacatttt ctcaatatgg
     1141 gtctttacag gagatatacc taatcgaacg taaatactgc cggactgggc ggcatctgcg
     1201 gaaccgggct gaacggcacg gatcagcatc ccgggttgaa cgtgcggacc cagacaaaag
     1261 ctcttatctt gtccgggcaa acaagagaag aatgatgaat cgattggggg agcgcctcgc
     1321 cgctgtcctc gtgctctctt tcatcctcat cgcacccgtt gtacagccgc cgtggctcct
     1381 ggcaatcatg gtggtgctct tttcaatggt gctctttctc atcagaaaga cccggttcct
     1441 tgcgatggcg ctcatcccca tcgccatcct ctatggcgtc tcaatccttc ccctcttggt
     1501 cttctcctgc accctcacca tcatggtgat gggcgagctt gcattcaggg ggacggatga
     1561 gcgtctttac tcgtacatcg ggtatattgc cgcggcactg gtgggctgcg ttctcgtgat
     1621 gggctacctc aacacatggg tcccccttgt cgtgctcttc ggggtcgtcg tcgcagtcct
     1681 cctgagggcg attctcaggg aacgggaaga tgcgctgatg atcgaggcgc ttggcatcgc
     1741 catgacgatg ttcctcatct acgaactgaa ttaccatgca gacatggccc tcatcgggat
     1801 tgctgtcgca atcgccttct cttttgcata tttctcgtac cgggcgggga ccgcggacgt
     1861 gagcgggctc ttctcggctg cactgatagg aatcatcctg ataatctttg ccgacatccg
     1921 ctggttcctg gtcgtcctgg cattcttcat catgggatcg gccagtacaa aataccggta
     1981 tgactataaa ctccggatcg gcgttgagca ggttcacggt ggcgcaaggg gatacctgaa
     2041 tgtctttgcg aacgggagcg tctccgcggc cgcggcagtc ctgtggggtg tgaccggctc
     2101 tccggtattc ctcgcccttt tcgtgggcag catagccacc gcggcaggcg ataccatggc
     2161 aagcgagatc ggggtgacgg gtggcagccc gtttctgatc accacgttcg agagggtgcc
     2221 ggcggggacg aatggcggcg tgacgctcac cggggaggga gtggcgatcg cgggagcctt
     2281 cattgtctct ctgcttgcct ttctcctcgg gattattgac ctgccgactg cggcagcctg
     2341 catggttgcc ggattcatcg gcacaaacat tgacagcgtg gtgggagcgt tgatggagaa
     2401 ccccgggttt gtcgggaatg cggggacgaa cttccttgcg acgctt
//
LOCUS       DAOQTR010000356         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_52724_length_2440_cov_3.029769, whole genome shotgun sequence.
ACCESSION   DAOQTR010000356 DAOQTR010000000
VERSION     DAOQTR010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52724_length_2440_cov_3.029769"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKN85_08520"
     CDS             complement(<1..458)
                     /locus_tag="PKN85_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNT44493.1"
                     /translation="MKKLFVLFIALSLCLVGYTAQAEARNASVKKAPARQNREAEPPC
                     KSFIVVEAQGGKMIDGSNINEKRAPASITKLMVTYVVLGRLATGGIKLTDKVTISKEA
                     SKIGGSQVYLKEGEVFTLEELMKAMMVASGNDAAYAISGYVAGSKDEMVNL"
     gene            complement(478..1233)
                     /locus_tag="PKN85_08525"
     CDS             complement(478..1233)
                     /locus_tag="PKN85_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNT44494.1"
                     /translation="MFSIPILFIVVIVVFFLFSAIKILNEYERGVIFRLGRVLGMPKG
                     PGLIILIPIIDRMVKVSLRTVVLDVPPQDVITRDNVSIKVNAVVYFRVVDPLKAIIDV
                     ENYLYATSQLSQTTLRSVLGQAELDDLLSHREQLNDRLQDILDTHTEPWGIKVSNVEV
                     KNVDLPQEMQRAIARQAEAERERRAKVISAEGEFQAATKLSEASEILSQNPMALQLRY
                     LQTLIEISTEKNSTIVFPLPIDLIKMFMDKLSK"
     gene            complement(1238..>2440)
                     /locus_tag="PKN85_08530"
     CDS             complement(1238..>2440)
                     /locus_tag="PKN85_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNT44495.1"
                     /translation="RIQGAINPAVAGFLGECIEKAGKADAEALVVLLDTPGGLDSSMR
                     DIVKEIMDAPVPVIVYVAPSGARAASAGAIILLASHVAAMAPGTNAGAAHPVTIGKEK
                     ADEVMMKKVVSDAEAYSKSIAAKRGRSVDFAAKAVTESISVTAKEALAGKVIDVVADS
                     TDDLLARIDGRTVETRKGKVVLHTKGAKKIEIAMPFKFRFLAYVSDPNVAYILMMIGI
                     YGILFEFYSPGTIFPGVIGGISLILALYAFQAIPISFAGLALILLGIVFFILEVKIVS
                     HGLLGIAGIVSVVIGSVMLVDLPGSPLAISFTTILIVAIISAIFVFGILSFAVKAQLS
                     RVKTGSEGLLGEMGTARTKIAAGGRGKVMVHGELWNAHSDEAIGEGEEIVVTAMEGLV
                     IKVRKKGG"
BASE COUNT          492 a          750 c          648 g          550 t
ORIGIN      
        1 aggtttacca tctcgtcctt gctgccggcg acatacccgc tgatggcgta ggctgcgtcg
       61 ttgccggagg cgaccatcat cgccttcatg agctcctcca gggtgaagac ctcgccctcc
      121 ttgagataga cctggctgcc cccgatcttc gacgcctctt tcgatatggt taccttgtcc
      181 gtcagcttga tccccccggt cgcaagcctt cccaggacga cgtaggtcac catgagcttc
      241 gttatgctcg cgggggcccg cttttcgttg atgttactcc cgtctatcat cttgccgccc
      301 tgggcctcga ctacgataaa ggacttgcag gggggttcgg cctccctgtt ctgccgggcg
      361 ggcgctttct tgactgatgc gtttctggcc tcggcctgcg ccgtataacc gacgaggcac
      421 aacgataggg cgataaacag tacgaacagt tttttcatgt cctccaaatt ccttgcctta
      481 cttgctcaat ttgtccataa acatttttat gaggtctatg ggcaacggaa agactatggt
      541 cgagttcttc tccgtcgata tctctatcaa tgtctgcagg tacctcaact gcaaggccat
      601 cgggttttgg gagaggatct ccgatgcctc ggaaagcttt gtggccgcct gaaactcacc
      661 ttcggcgctg ataaccttcg cccttctctc cctttccgcc tccgcctggc gcgcaatggc
      721 ccgctgcatt tcctgaggca gatctacatt cttcacctcg acgttggaga cttttatgcc
      781 ccagggttcc gtatgggtat cgagaatatc ctgaagcctg tcgttcaatt gctccctgtg
      841 tgaaagcagg tcgtcgagtt cggcctgacc gagaacgctt ctcagggtgg tctgggaaag
      901 ctggctcgtc gcgtagaggt agttctccac gtcgatgatg gccttcaggg gatcgacgac
      961 acggaagtag acgacggcgt ttaccttgat ggaaacgttg tcgcgggtta tgacatcctg
     1021 cggaggtacg tcaagcacaa cggtccgcag gctcactttg accatgcggt caatgatggg
     1081 gatcaggata atgaggcccg gtcccttcgg cataccgagc actctgccga gcctgaagat
     1141 cacgcccctt tcatactcgt tgagtatctt tatcgcactg aaaagaaaga agacaacgat
     1201 aacgacgata aacaggatcg gaatggaaaa catactctta ccctcctttt ttccttacct
     1261 tgatgacaag cccttccatc gcggtgacca cgatctcctc gccttcaccg atcgcctcgt
     1321 cactgtgggc attccagagt tcgccatgga ccatgacctt ccccctgcca ccggcggcga
     1381 tcttcgtcct ggccgtcccc atctccccga gaagcccctc ggagccggtc ttgaccctgg
     1441 acaactgcgc ctttacggca aaggagagta tgccgaaaac gaatatggcc gatatgatgg
     1501 cgacaatgag gatggtcgtg aaagatatgg caagcgggct gcccggaaga tcgacgagca
     1561 tgaccgaccc gatgacgacg gacacgatgc ccgcgatgcc caaaagtccg tggctgacta
     1621 tcttgacctc caggataaag aatacgatac ccaggaggat caatgcaagc ccggcaaaac
     1681 tgatcggtat cgcctgaaag gcgtagagcg ccagaatgag agagatgcca ccgataacgc
     1741 cggggaagat cgtacccggg ctgtagaact cgaaaaggat gccgtagatc ccgatcatca
     1801 tgagtatgta cgcaacgttc ggatcgctca cgtaggccag gaaccggaac ttgaagggca
     1861 tcgcgatctc tatcttcttc gcgcccttcg tgtgcagcac gacctttcct ttcctggtct
     1921 ccacggtcct tccgtctatc cgcgccagaa ggtcgtccgt agaatcggcc acgacatcaa
     1981 tgaccttccc tgcgagggcc tccttcgcgg tcaccgagat gctttccgtg accgcctttg
     2041 ccgcaaaatc gacactcctt ccgcgcttcg cggctatgct cttcgaatac gcctccgcgt
     2101 cgctgaccac cttcttcatc atcacttcgt cggccttttc cttgccgatg gtgacaggat
     2161 gagcggcgcc cgcgttggtc ccgggggcca tcgccgccac gtgggaggcc agaaggatga
     2221 tggcgcccgc cgaggccgcc ctcgcccccg atggggcgac atagacgatg acgggaaccg
     2281 gcgcgtccat gatctccttc acgatgtccc gcatggacga atccaatccc cccggcgtat
     2341 cgaggaggac cacaagggcc tcggcatccg ccttgccggc cttctcaata cattccccca
     2401 ggaagcccgc cacggccgga ttgatggcgc cctgtatgcg
//
LOCUS       DAOQTR010000357         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_52758_length_2439_cov_3.335151, whole genome shotgun sequence.
ACCESSION   DAOQTR010000357 DAOQTR010000000
VERSION     DAOQTR010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52758_length_2439_cov_3.335151"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PKN85_08535"
     CDS             complement(<1..719)
                     /locus_tag="PKN85_08535"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNT44496.1"
                     /translation="MANVGVVGVQWGDEGKGKIIDYLSGFADIIVRFQGGANAGHTIV
                     IGDKKVILHLIPSGILREKTYCVIGNGVVMDPEVLEKEIDELKGLGYLQDERRLMVSG
                     YTHVIMPYHKKLDALRESRSAKKIGTTGRGIGPAYEDRVSRMGIRVVDLIDRKVFREK
                     LKANLEIKNFLIRRYYKDEPLMIRDILKKFEPYRKMLKRHVTDSTSFLHKAMAKKKRI
                     LFEGAQGTFLDIDHGTYPYVTS"
     gene            complement(834..1904)
                     /gene="ruvB"
                     /locus_tag="PKN85_08540"
     CDS             complement(834..1904)
                     /gene="ruvB"
                     /locus_tag="PKN85_08540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNT44497.1"
                     /translation="MIQEENSTEISELYRKEENGGEEGIVTLRPSRLHEYVGQDTIVE
                     TLQIAIEAALKRGEPLEHILFNGPPGLGKTTLAHIIANEMGTRIVTSSGPALEKGGDL
                     MGLLTHLEKGDVFFIDEIHRIPKIVEEFLYPAMEDFAVDFIFDKGIHARTHRYRLEQF
                     TLIGATTRSGLLSSPLRERFGIVRDLDFYRDEDLVKIIKRSASILGVTVDDEGAHETA
                     KRARGTPRVANRLLKRVRDYAEVRAGGAITKNVAIDALSLEGIDDHGLGETDRKLLKT
                     IILNYKGGPVGIEALAATLQLESDVLIEVVEPFLLKSGFIIRTSQGRKASERAFSHLG
                     MPLSGAPATGGLFDSTPTRKDR"
     gene            complement(1901..>2439)
                     /locus_tag="PKN85_08545"
     CDS             complement(1901..>2439)
                     /locus_tag="PKN85_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNT44498.1"
                     /translation="DVLIPAFVMSEIRRSKKAEDDLSLYISFHQTERQPKPVLVGFRH
                     ELDREFFELFITVEDIGPMAAVKALTKSIREIARHIEEKDVKSLRKLKGIGERKADKI
                     VATLKGRVAKYALMPETAAASPIAEDFVKEVEQVLVTQLGHKVLEARQMIEEAMKRNP
                     RIATSEELFEEVYRGQRQ"
BASE COUNT          425 a          735 c          686 g          593 t
ORIGIN      
        1 gaggtgacgt aagggtacgt cccgtggtcg atatcgagaa aggtgccctg ggccccctcg
       61 aagagtatcc ttttcttttt cgccatcgcc ttgtggagga aggacgtcga gtcggtgacg
      121 tgcctcttga gcatcttccg gtagggctcg aatttcttca ggatatccct gatcatcagc
      181 ggctcgtcct tgtaatagcg gcggataagg aagttcttga tctccaggtt ggccttcagt
      241 ttctctcgaa agaccttcct gtcgatgagg tcgacgacgc ggatgcccat cctgctcacc
      301 ctgtcctcgt aagcgggacc gatccctcgt cccgtcgtgc ctatcttctt cgcgctcctc
      361 gattccctga gggcgtcgag ctttttatgg tagggcatga tgacatgagt atacccgctc
      421 accatcagcc gcctttcatc ctgaagatag ccgaggccct tcagctcgtc tatttccttc
      481 tccaggacct cggggtccat gacaacgccg ttgccgatga cacagtatgt cttctcgcgg
      541 agtatgcccg aagggataag atggaggatg accttcttgt cgccgatgac gatggtatga
      601 ccggcgttcg ccccgccctg gaaccggacg atgatgtccg cgaacccgct gagatagtcg
      661 atgatctttc ctttgccttc gtcaccccac tgaaccccga caactcccac attggccatg
      721 accttctcct gtgctttttc gttgaagaac atatcttttt aatatactat cccgctaaaa
      781 tcaaggctaa tcggggaaag gcggggcttt cggggaatgc ggggaggatg tgtctatctg
      841 tctttcctgg tgggtgtgct atcgaacaac ccacccgtgg ccggcgctcc tgaaaggggc
      901 atgccgagat gggagaaggc cctttccgag gccttcctgc cctgggaagt gcggatgatg
      961 aaccccgatt tcagcagaaa gggctcgacg acctcgatga gcacgtccga ctccagctgc
     1021 agtgtcgcgg caagggcctc gatgcccacg ggaccaccct tgtaattgag gatgatggtc
     1081 ttgaggagct tcctgtccgt ctcgccgagg ccgtggtcgt cgatgccttc aaggctcagg
     1141 gcgtcaatgg ccacgttctt cgttatcgcc ccaccggccc ggacctccgc gtaatcacgg
     1201 acgcgcttga ggagcctgtt cgccaccctg ggggtccccc tggcacgctt tgccgtctcg
     1261 tgggccccct cgtcgtcgac ggtgacgccc agtatcgagg ccgagcgctt tatgatcttc
     1321 accaggtctt cgtccctgta aaaatcgagg tcccgcacga tgccgaaacg ctctctcagc
     1381 ggcgatgaca gaagacccga gcgagtcgtc gcgcctatga gggtgaactg ctcgaggcgg
     1441 tacctgtgcg tcctcgcgtg gatacccttg tcgaagatga aatcgacggc gaagtcttcc
     1501 atggcgggat agaggaactc ctcgacgatc ttgggtatgc gatggatctc gtcgatgaag
     1561 aagacgtccc ccttttcaag gtgcgtcagg aggcccatga ggtcgccgcc cttctccagc
     1621 gccgggcccg aggatgtcac gatccgtgtg cccatctcgt tggcgatgat gtgcgccagc
     1681 gtggtctttc caagccccgg gggcccgttg aagagtatgt gctccagggg ctcccccctt
     1741 ttcagggccg cctcgatggc tatctggagg gtctccacga tggtgtcctg gccgacatac
     1801 tcatgcaggc ggctcggccg cagagtgacg atgccttctt cgcccccgtt ctcttctttc
     1861 ctgtaaagct cggagatctc cgtggagttc tcttcctgta tcattgcctc tggcccctgt
     1921 agacttcctc aaaaagctct tccgacgtgg cgatgcgagg gtttctcttc atcgcctcct
     1981 ctatcatctg ccgtgcctcc agcaccttgt ggccaagctg ggtgacaaga acctgttcca
     2041 cctctttcac gaaatcttcc gcgatcggcg aggctgcggc cgtctccggc atgagggcat
     2101 acttcgcgac cctgcccttc aaggttgcca cgatcttgtc ggccttcctc tcccctatcc
     2161 ctttgagctt gcggagggac tttacgtcct tttcctcgat atggcgcgct atttcccgta
     2221 tggacttcgt gagggctttg acggcggcca tggggccgat atcctccacg gtgatgaaaa
     2281 gctcgaagaa ctccctgtcc agctcatgtc tgaagccgac gaggacgggc ttgggctgcc
     2341 tttcggtctg gtggaaggat atgtagaggc tcaggtcgtc ctcggccttc ttcgaccttc
     2401 ttatctcgct catgacgaat gccgggatga ggacatcat
//
LOCUS       DAOQTR010000358         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_52974_length_2433_cov_3.121531, whole genome shotgun sequence.
ACCESSION   DAOQTR010000358 DAOQTR010000000
VERSION     DAOQTR010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52974_length_2433_cov_3.121531"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKN85_08550"
     CDS             complement(<1..518)
                     /locus_tag="PKN85_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNT44499.1"
                     /translation="MLKESLISEFQKIVGKDNVLTSPEALKAYSYDGTTSWVHEPDVV
                     IFPTSTDEVSRIMKLANAEKIPVTPRGGGTNVSGGSVPWHGGVVLVMTKFNQILKVDK
                     ENLTATVEPGVVLQDLTLRLAKEGLFFPPDPQSFLGATMGGIISENAGGPAGVKYGVT
                     KQYVLGVEVVLP"
     gene            748..1662
                     /locus_tag="PKN85_08555"
     CDS             748..1662
                     /locus_tag="PKN85_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNT44500.1"
                     /translation="MDILETIAPIFIIIAFGYVLKSRKMLSDAFVSEANRFVFRFPLP
                     FLIFTGILKANMKDVGWLSVLSLIIPTAMVMALAALSAVVARYQGGKRGSFIQTTFHG
                     NVTYVGLAVIFYLLGDEGLERGSLLVGMLILFNNAGAVLALSLSSGKHRNVMKTALSI
                     LTTPVIVATFAALILAFLKVTMPAVIMKSITIVANIALPMALIIIGCSISPEMLKKTI
                     KPAALAATLKVIVLPALAVLFCRIFAIDLKEALPAIILLATPTAITAYIMARELGGDT
                     DLASGTVTLTTLVSPLTYLGWIWLLKTA"
     gene            complement(1801..>2433)
                     /locus_tag="PKN85_08560"
     CDS             complement(1801..>2433)
                     /locus_tag="PKN85_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT44501.1"
                     /translation="RYRPVEIAPETFVIQATIGEGVAPLAVHLNSMLIRGAEPVVVHP
                     GYSFFRKLKDYDRELRARFIEDLGTIQEEARSLGIRIALENVFMSYFYFQELDEFAAI
                     SEAVPGIGVTLDIGHAYISKCAAKHPDPEGAIIDDVRRLGVENLFHVHLHNNSGSRDD
                     HDFVEGSIDMARVLKGLTDLGYAGKVVIETLDIERLGFPAVLQKLEQIAP"
BASE COUNT          485 a          737 c          651 g          560 t
ORIGIN      
        1 ctcggaagga ccacctcgac acccagaaca tactgttttg tgacgccata tttcacgccg
       61 gcaggaccgc ctgcattctc ggatatgata ccgcccatcg tcgcaccgag gaaactctgc
      121 gggtccggcg ggaagaacaa accttccttc gcgagccgga gggtgaggtc ctggaggaca
      181 acgcccggct cgaccgtggc ggtcaggttt tccttgtcaa ccttgagtat ctgattaaac
      241 ttcgtcatga ccaggacaac gccgccatgc cagggaacag accctccact gacgtttgtg
      301 ccgccgcccc tgggggttac gggtatcttt tcagcgttgg ccagcttcat gatccgggac
      361 acttcatccg tagacgtagg gaagatgacg acatccggct catggaccca gcttgtcgtt
      421 ccgtcgtagg aatacgcttt cagagcctct ggcgaggtaa ggacgttgtc cttgcccaca
      481 atcttttgaa attcgcttat caacgactct tttaacatcc cttctacccc ctgagtttga
      541 tattgttact aatttcactt tcttatatcc ttttcccctt tcctttgtca acaaaaatca
      601 caaggccaaa aatgctctga tatcaagtaa ttggccacag ttaaggcctt gcggaagaat
      661 gggaaaaatg gtatggaatc gtatgtttcc catagtgaac aaatggaccg cacatcgcct
      721 gtacatcgac gttttctggg ggtatacgtg gatatccttg agaccatagc tccgatcttc
      781 atcatcatcg ccttcgggta tgtcctgaag agcaggaaga tgctttccga tgccttcgtt
      841 tcggaggcga accgtttcgt gttccgattc cccctcccct ttctcatctt cacggggatc
      901 ctgaaagcga acatgaagga cgtgggatgg ctgtcggtcc tctccctcat cataccgacg
      961 gccatggtta tggccctggc cgccctttcg gcggtagtgg ccaggtacca gggcggcaaa
     1021 cggggaagtt tcatccagac gacgttccac ggcaacgtga catacgtcgg ccttgccgtt
     1081 atcttctatc ttctcgggga cgaaggactc gaaaggggga gcctcctcgt cggtatgctg
     1141 atactcttca acaatgccgg agccgtgctg gcgctctccc tgtcttccgg gaaacaccgc
     1201 aacgtgatga aaacggcgtt gtcgatcctc accacaccgg tcatcgtcgc caccttcgct
     1261 gccctcatcc tcgccttcct gaaagtgacc atgcctgccg tcatcatgaa gagcatcacg
     1321 atcgtggcca acatagccct tcccatggcg ctcatcatca tcggctgctc catttctccg
     1381 gagatgctga agaagaccat caagccggcc gctctcgccg cgaccctcaa ggtcatcgtc
     1441 cttcccgcgc tggcggtcct gttctgccgt atcttcgcga tagacttgaa agaggccctt
     1501 ccggcgatca tcctcctcgc gacaccgacg gccatcacgg cctacatcat ggcacgtgag
     1561 ctcggcggcg acaccgacct cgcctccggc acggtgacac tgacaaccct cgtctcaccc
     1621 ctgacgtatc tggggtggat atggctctta aagacggctt gaatcgcttt aattgtttga
     1681 gagtgaaagg caaaaagatc tcccggtggg gtccggtgcg tcgcacggcg aggatcctct
     1741 tgcctgctcc ttctcaaccc tcaagcgact caagcggttc aagccgttca agcgcgcccg
     1801 tcagggcgcg atttgttcca gtttttggag gactgccggg aagccgaggc gttctatgtc
     1861 cagggtctcg atgacgacct tgccggcgta gcccaggtcc gtgagaccct ttaagaccct
     1921 cgccatgtct atcgaaccct ccacgaagtc gtggtcgtcg cggctgccgc tgttgttgtg
     1981 caggtggaca tggaagaggt tttccacgcc caatcggcgg acatcgtcga tgatcgcgcc
     2041 ttcggggtcg ggatgcttcg ccgcgcattt cgagatatag gcgtggccga tgtcgagggt
     2101 gacccctatc ccgggaacgg cctcactgat ggcggcgaac tcgtccagtt cctggaagta
     2161 gaaataggac atgaacacgt tctccagcgc tatcctgatc ccgagggacc tggcctcttc
     2221 ctgaatggtg ccgaggtcct cgatgaatcg ggccctcaac tccctgtcgt aatccttgag
     2281 cttgcgaaag aaggagtatc ccgggtggac cacgaccggc tcggcacccc ggatcagcat
     2341 cgagttcagg tgcacggcga ggggcgcgac gccttcgccg atcgtggcct ggatcacgaa
     2401 cgtctccggc gcgatctcca ccgggcggta gcg
//
LOCUS       DAOQTR010000359         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_53034_length_2431_cov_4.087542, whole genome shotgun sequence.
ACCESSION   DAOQTR010000359 DAOQTR010000000
VERSION     DAOQTR010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53034_length_2431_cov_4.087542"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1950
                     /locus_tag="PKN85_08565"
     CDS             <1..1950
                     /locus_tag="PKN85_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44502.1"
                     /translation="RRRMSCRRWTMPWGTEMGLPLKNSEYLERVKSFQSSSEKYMESH
                     LHGQWKKNRNMFKLIHENQALMTDPRYRHRSKLYIPKARNAVMRKLAAFLSTYFSNPD
                     VVSVKPRRKRDPVMAQAAALLHSCMNYRLKNTMNFFLNTLFAWLDIMQYGRGAVCMGW
                     DYQEETTKRKETLPLKDEDGIPEDIIEQTVETVKVLKDEPTLRHVPLWNLFLAPTADP
                     IDPVNSSPCLVERIPVFTYDAVKKFKGSEWNAPKDVDLEEKGKLDEFLEGYAWKPSSI
                     EDEIYGEDKDGFTKNALWQQIEVWKCFVNVDGEDVYFLSLRGEHMLTEPERVEDKWPC
                     RGRPYILGGIIPDSGLVYWPSFLEIVEGLQREMNAIRNQRRDNVTLALNKKLLVRRNT
                     GIDTNSLLYSRPGAPILGDDIGEMAVRELRYEDVTSSSYKEQQINEQAFEEATGITPY
                     NMGTQRPGMNPTATGVSILTEEANTVNAMELRIINETFMVPMLRMHAQFEQAFESEEI
                     MRYVADEEGIDFDSVWTDEAIEGEYHIEVDAGIGATSRELRLRNLGMMLDRAMAINTQ
                     YGAPVMNLIQFARDAMPLAGFENPDKYVNENVLKMVMSKFGQVAGMNLMDPTQGPARL
                     ISPANSGGSQVESMQGYGGDKQIYR"
     gene            1922..2248
                     /locus_tag="PKN85_08570"
     CDS             1922..2248
                     /locus_tag="PKN85_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44503.1"
                     /translation="MAETSRSTGDVSLDEYIGELLSRADVADRLKACRGDCPLLGIIR
                     DLSHNAMIDLVQVDPRDTIKVIQRQEVAKLYNTIDNAIDGKVLEGHAAEDDITTMEEE
                     GYGSKG"
     gene            2232..>2431
                     /locus_tag="PKN85_08575"
     CDS             2232..>2431
                     /locus_tag="PKN85_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44504.1"
                     /translation="MAAKDKGLQDRQADELNTPVEDGLEADSDIISSRDRDVFLKQYQ
                     EHERSELRDQGFDEDEIRAILG"
BASE COUNT          571 a          692 c          786 g          382 t
ORIGIN      
        1 cggcgccgga tgagctgccg ccgatggacg atgccgtggg gtactgagat gggacttccc
       61 ttgaaaaaca gcgaatatct tgagcgcgta aaatccttcc agtcatcctc cgagaagtac
      121 atggagagcc acctgcacgg gcagtggaag aagaaccgca atatgttcaa gctcatccac
      181 gagaatcagg ccctcatgac cgacccgcgc tatcggcacc ggagcaagct ctacatcccc
      241 aaggcgcgca acgcggtcat gcggaagctg gcggcttttc tatcgactta cttctccaac
      301 cccgacgtgg tgagcgtaaa gccccgtagg aagcgcgatc cggtcatggc tcaggcggct
      361 gcgttgctcc attcctgcat gaactaccgg ctgaagaaca cgatgaactt cttcctcaac
      421 accctgttcg cgtggctcga catcatgcag tacggcaggg gcgcggtctg catgggatgg
      481 gactaccagg aggagacgac gaagaggaag gagactctgc ccctgaagga cgaggacggc
      541 atccccgagg atatcatcga gcagaccgtc gagacggtca aggtgctcaa ggacgagccg
      601 acgctgcggc atgtgccgct gtggaacctg ttcctcgcgc cgaccgcaga cccgatagac
      661 ccggtcaact catcgccctg cctcgtggag aggatacccg tctttaccta cgatgcggtc
      721 aagaagttca agggcagcga gtggaacgcg cccaaggacg ttgacctgga ggagaagggc
      781 aagctcgacg agtttctgga gggctatgcg tggaagccgt cctccatcga ggacgagatc
      841 tacggcgagg acaaggacgg gtttacgaag aacgccctgt ggcagcagat cgaggtctgg
      901 aagtgctttg tcaatgtgga cggggaggac gtgtatttcc tctcgcttcg cggcgagcac
      961 atgctcaccg agccggagcg cgtcgaggac aaatggccgt gcaggggcag gccgtatatc
     1021 ctgggcggaa tcatacccga ctccgggctc gtgtactggc cctcgttcct cgaaatcgtg
     1081 gaagggctcc agcgggagat gaacgcgatt cggaaccagc gcagggacaa cgtgactctg
     1141 gcgctcaaca agaagctgct ggtcaggcgc aacaccggga tcgacacaaa ctcgctgctc
     1201 tactcgcgcc ccggcgcccc catcctgggc gacgacatcg gggagatggc cgtgcgcgaa
     1261 ctgaggtacg aggacgtgac ctcaagctcg tacaaggagc agcagatcaa cgagcaggcg
     1321 ttcgaggagg caaccgggat caccccgtac aacatgggta ctcagcgtcc cggcatgaac
     1381 ccgaccgcga ccggcgtgtc catcctcacc gaggaggcga acacggtcaa cgccatggag
     1441 ctcaggatca tcaacgagac gttcatggtc cccatgcttc gcatgcacgc tcagttcgag
     1501 caggcgttcg agagcgagga gatcatgcgc tacgttgcgg acgaggaggg gatcgacttc
     1561 gactccgtgt ggacggacga ggcgatagag ggggagtatc acatcgaggt tgacgccggg
     1621 atcggcgcca cctcgcggga gctgaggctc aggaacctgg gcatgatgct ggatcgcgcg
     1681 atggcgatca acacgcagta cggcgccccg gtcatgaacc tcatccagtt cgccagggac
     1741 gcgatgcccc tggcggggtt cgagaacccg gacaagtacg tcaacgagaa cgtgctcaag
     1801 atggtcatga gcaagttcgg gcaggtggcg ggcatgaacc tcatggaccc gacacaggga
     1861 ccggctcgac tcatcagtcc ggcaaacagc ggcggaagcc aagtggagag catgcaggga
     1921 tatggcggag acaagcagat ctacaggtga cgtgagcctc gacgaataca tcggcgagct
     1981 gctcagccgt gcggacgttg cggacaggct caaggcatgc aggggcgact gccccctgct
     2041 cggcatcatc cgcgaccttt cgcacaatgc gatgatcgat ctcgtgcagg tggaccccag
     2101 ggacacgatc aaggtcatcc agcggcagga ggtagcgaag ctctacaaca cgattgacaa
     2161 cgcgatagac ggcaaggtcc tggagggcca cgccgcagag gacgatatca cgaccatgga
     2221 ggaggaagga tatggcagca aaggataagg gacttcagga ccgtcaggcg gacgagctga
     2281 acacccctgt cgaagacggg ctggaagccg attccgacat catttcaagc agggaccgcg
     2341 acgtgttcct caaacagtac caggagcacg agcgaagcga gctcagagac caggggttcg
     2401 acgaagacga gatccgggcg atcctcgggg a
//
LOCUS       DAOQTR010000360         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_53382_length_2421_cov_3.784024, whole genome shotgun sequence.
ACCESSION   DAOQTR010000360 DAOQTR010000000
VERSION     DAOQTR010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53382_length_2421_cov_3.784024"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1177)
                     /locus_tag="PKN85_08580"
     CDS             complement(131..1177)
                     /locus_tag="PKN85_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44505.1"
                     /translation="MKVKTYTFDDIRKGMDVIKEQFGPDTIIMDIKQNDHNGNGWTKK
                     GCEISIAVENDPAVTMETDLGEIRKKTEAIWSDAARYLTERLVSIETDMLLDRLKAYP
                     MPLKILHDRLVRAGLDRHITMALLSEVFAEIGSIAENSTKALFFTKAKLGKRIPLCDA
                     MSAEEPLLLIGPAGSGKTETAKKLARLMHGKDLHPSIIAFDPVRRGTYEELRVFSEQT
                     AIPFQFAASIEDLRRKAREGGGKKLIDVTGHMTYQGEVVDNFREMKKLVVLPAGMRDE
                     TMDHYLGIMDGAAVSGLIFTKLDEEERLGHLCNSLLRLSRPIAALTRGAGMGDILIPD
                     QETFGRILIEGKAW"
     gene            complement(1167..>2421)
                     /locus_tag="PKN85_08585"
     CDS             complement(1167..>2421)
                     /locus_tag="PKN85_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNT44506.1"
                     /translation="LGSDVVAQVFTQPRALVLASFVILSIGMVPGIPLVPFLILSSIT
                     YGVSHVMKKEPDEDLPAVPDASDESREKGELIQPLEILEVEIGYGLIPIVDAEQNGEL
                     LDKIRAIRKQIAVELGIVVPPMKLRDNLQLGASEYVVLLKGIEMGRGSLISGNLLAMG
                     PEGKERPADGIPTKEPVFNLDAYWIAEKDRDAYISEGFTVVDHATVIATHLTELVRNN
                     AHELLTRQETQKLIDNIAPTHQKVIEEISQNQVNVGVVQKVLQNLLREQVSIRDLITI
                     LEAVADASSSTKDPDSITEYVRQKMARSILKPYLVDGILNIHILEKTLEERLLSSVQP
                     TDQGSILALDLGFSQKLIEKIGNEAKKAMLQNYQPVILVHPLLRGRLRRFLDRYIQGI
                     TVISHNEIPPQIRIQSVGVIRINEG"
BASE COUNT          415 a          708 c          714 g          584 t
ORIGIN      
        1 gaaaggtcgg cgtcgaggat aaaggtggac ctcttcctgt tcccgagaag ataggccatg
       61 ttcgacgcta tcgaagattt gcccactccg cccttgccgc tcgttatcgc tatcgttctg
      121 cttccgtttc ctaccatgcc ttcccctcga tgagtattct gccgaatgtc tcctggtccg
      181 ggatgaggat gtcccccatg ccggctcctc ttgtcagggc cgcgatgggc cgcgacagcc
      241 tcaggagact gttgcacagg tggccgaggc gctcttcctc gtccagcttg gtgaatatga
      301 gacccgagac ggccgccccg tccatgatgc cgagatagtg gtccatcgtc tcgtccctca
      361 ttcccgccgg gagaacgaca agtttcttca tctcccggaa attgtcgacc acctccccct
      421 gataggtcat gtggcccgtg acgtctatga gcttctttcc gcccccctcg cgtgccttgc
      481 ggcgaaggtc ctcgatgctc gccgcgaact ggaaggggat ggccgtctgc tcggagaaga
      541 cacggagttc ctcgtaggtg cctctcctga ccgggtcgaa ggcgatgatg gacggatgga
      601 gatctttccc atgcatgagc cgggccagtt tcttcgccgt ctccgtcttt cccgatccgg
      661 ccggcccgat gaggagcagc ggctcttccg cactcatggc gtcacacagg ggtatgcgtt
      721 tgcccagttt cgccttggtg aaaaagaggg ccttggtgct gttctccgct atggacccta
      781 tctcggcgaa cacctcggag agaagggcca ttgtgatgtg tctgtcgaga ccagccctga
      841 ccagtctgtc gtgaagtatc ttgagcggca tgggataggc cttgagcctg tcgagaagca
      901 tgtccgtctc gatggacacg agacgttccg tgagataccg tgccgcgtcg ctccagatgg
      961 cctccgtctt cttcctgatc tccccgagat cggtctccat ggtgacggcc gggtcgttct
     1021 caaccgctat ggatatctcg catcccttct tcgtccagcc gttgccgttg tggtcgttct
     1081 gcttgatatc catgatgatg gtgtcagggc cgaactgctc cttgatcaca tccatgccct
     1141 tgcggatatc gtcgaacgta taggtcttaa ccttcattga tccttatgac ccccactgat
     1201 tggatcctta tctgaggtgg tatctcgtta tgggagatga ccgttattcc ctgtatgtac
     1261 cggtcgagga accttctgag ccttcccctc agcaggggat ggacgagaat gacgggctgg
     1321 tagttctgaa gcatggcctt cttcgcctcg ttgccgatct tctctatcag tttctggctg
     1381 aaaccgagat cgagtgcgag gatggacccc tggtccgtgg gctgtacgct ggaaagaagc
     1441 cgctcctcga gggtcttctc caggatgtgg atgttgagga tgccgtccac gaggtagggc
     1501 ttgaggatgc ttctcgccat cttctgacgg acatactcgg tgatgctgtc agggtccttc
     1561 gtcgacgacg acgcgtcggc caccgcctcg aggatcgtta tgaggtcacg gatcgagacc
     1621 tgttcacgca gaaggttctg aaggaccttc tggacgacgc ccacgttgac ctggttctgg
     1681 gagatctcct cgatcacttt ctggtgcgtc ggggcgatat tgtcgatcaa tttctgggtc
     1741 tcctgcctgg tcaggagttc gtgggcgttg ttcctcacca gttccgtcag gtgcgtggcg
     1801 atgaccgtgg cgtggtcgac aacggtgaag ccttccgata tgtaggcatc gcggtccttt
     1861 tcggcgatcc agtacgcatc gaggttgaag accggctcct tcgtgggaat gccgtccgcc
     1921 ggcctttcct ttccctcggg acccatggca aggaggttcc ccgagatgag gctgccgcgg
     1981 cccatctcta tgcccttcag caggacaaca tattccgacg cgccgagctg caggttgtcc
     2041 ctgagcttca tgggcgggac cacgatgccc agttcgacgg ctatctgctt gcggatggcc
     2101 cgtatcttgt cgagaagttc cccgttctgt tccgcgtcca cgatgggtat gagaccgtag
     2161 ccgatctcca cctccagtat ttcgaggggc tggataagct cgcctttctc cctcgactcg
     2221 tcggaagcat cgggtacggc aggcaagtcc tcatcaggct ccttcttcat gacgtgactg
     2281 accccgtacg tgatggagga gaggatgaga aagggcacca gcgggatgcc gggcaccatg
     2341 ccgatggaga gtatgacgaa ggaggcgagc acgagcgccc tgggctgggt gaagacctgg
     2401 gccacgacat cgcttcccag g
//
LOCUS       DAOQTR010000361         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_53572_length_2416_cov_4.009742, whole genome shotgun sequence.
ACCESSION   DAOQTR010000361 DAOQTR010000000
VERSION     DAOQTR010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53572_length_2416_cov_4.009742"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>2416)
                     /locus_tag="PKN85_08590"
     CDS             complement(147..>2416)
                     /locus_tag="PKN85_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44507.1"
                     /translation="LASSIAADPTVVRASRSGDYRHVQAKLEALYKRIGRPFFTLFVL
                     DRKGIVRVDAQFPRQMGVNLSDRDYFLRAREGKTSVTGPVPARGPSTPGDPIVIVSAP
                     IREDNEFHGVVCIPFDPNFLGAIVSQVRTGKTGYAYLVNSEGLVLAHPKKEYVLRVRL
                     QDHPGTEEIYKLVQAGRTGTASYYSGGARKVAGLTTMQLTGWTVAFAQNRDEVMAPVN
                     RILWIMLVSGILFLIMTITTIVIVSSRLSSPVQRLLERMKKMTEHSTEIILQIGTDRK
                     IAFANPAFERITGTRPEEVIGTEPDFANPHNIPPDVIWDSLNSGTPWSGRVSLRAIDG
                     SPVTLDVMVMPLRNSNGTVEGYLEIGRDVTKELMFENKMQQSQKLEAIGTLAGGIAHD
                     FNNILSGILGYAELALITGKNDGETEKFLKEIIKAAQRAGELVGQILTFSRQTEVELR
                     PILPGPILEEASKLLRASAPASIDIRLRIDSRSAILAEPTQIHQIVMNLFTNAVHAIE
                     PEHGTITIELEDLVVDDDLAKAHPDMRPGRHIALSVSDTGSGMEQEIVDRIFEPFFTT
                     RSQGKGTGLGLSVVHGIVKELGGMIELKSQAGKGTTFSIYIPCTDMDAAGPPREAPPT
                     EKGRYRIVLIDDEEAILTTTGAILTQLGHKVTTFANGLDALEAVKEDPHDFDLVITDH
                     SMPKITGLEIARSLRENGIDIPVILTSGYLAHTMGDEIRDVGVSEVLTKPITTQQLTE
                     TIGRVLGDPRPIDPS"
BASE COUNT          426 a          694 c          733 g          563 t
ORIGIN      
        1 tccgcccccc cggcacgatc cgtcggtcgg gagggcctga aagcagctag cgtgctacta
       61 atgaaaaaaa ggtagcatcc actgcacctc gtgtcaacag aaatgttttc catcatggaa
      121 ggatcgaaaa aatgggggag ggggccctag cttgggtcga tgggccgggg gtcgccaagc
      181 accctgccta tcgtctcggt aagctgttgc gttgttatcg gttttgtcag cacttccgat
      241 acgccaacgt cgcgaatctc gtcgcccatg gtgtgggcga ggtagcccga ggtgagtatg
      301 acggggatgt cgatgccatt ctctctaaga ctccgtgcta tttcgagacc cgtgatcttt
      361 ggcattgaat ggtctgtgat cacgaggtcg aagtcgtggg ggtcctcttt tactgcttcc
      421 agggcatcga ggccgttggc aaaggtggtg accttgtggc cgagctgcgt caggatggcg
      481 cccgtggtcg taagaattgc ctcctcgtcg tctatgagga cgatccggta tctccccttc
      541 tcggtcgggg gggcttcccg gggtgggccg gcagcgtcca tgtccgtaca ggggatgtag
      601 atgctgaagg tcgtgccctt gccggcctgg cttttgagct cgatcatccc gcccagttcc
      661 ttcacgatgc cgtgcacgac agagagcccg aggccggtgc ctttcccctg ggatctcgtg
      721 gtgaagaagg gctcgaatat acgatccacg atctcctgtt ccatgccgct tcccgtgtcg
      781 gagacggata gcgcgatgtg tcttcccggt cgcatgtcgg gatgggcctt cgcgaggtcg
      841 tcatcgacca cgagatcttc gagttcaatg gtgatggttc cgtgttccgg ttcaatggcg
      901 tgcacggcgt tcgtaaaaag gttcatcacg atctggtgga tctgggtcgg ttccgccagg
      961 atggcagatc ggctgtcgat ccgcaaccgg atgtcgatgg aggccggtgc ggacgcgcgc
     1021 aggagcttcg aggcctcctc aaggatgggt ccgggcagga tcggtctcag ctcgacctcc
     1081 gtctgcctgc tgaaggtcag tatctgtcct accaactcgc ccgccctctg agcggctttt
     1141 ataatctcct tcaggaactt ttctgtctcc ccatcatttt ttcccgtgat cagggcaagt
     1201 tccgcgtaac cgaggatgcc actcaggatg ttgttgaagt cgtgggctat tccaccggcc
     1261 agagtgccga tggcctcaag cttttgcgat tgctgcatct tgttctcgaa catgagctcc
     1321 tttgtcacgt cccggcctat ttccaggtag ccctccactg tgccgtttga gtttctaagg
     1381 ggcatgacca tcacgtcgag agtgaccggg ctcccgtcaa ttgcccggag ggacaccctt
     1441 cccgaccagg gagttcctga attgagggag tcccagataa cgtccggcgg gatattgtgg
     1501 ggattggcga agtccggttc ggtgccgatc acctcctccg gccttgtccc cgttatcctt
     1561 tcgaaggccg ggttcgcaaa ggcgatcttc ctgtcggtcc ctatctggag gatgatctcc
     1621 gtggagtgtt ccgtcatctt cttcatcctt tcgagaagcc tctgcacggg agaactgagc
     1681 ctgctcgaaa cgatgactat cgtcgtgatc gtcatgataa ggaagagtat tccgctgaca
     1741 agcatgatcc acaggatcct gttgacgggg gccatcacct cgtccctgtt ctgggcaaag
     1801 gcgacggtcc aacccgtgag ctgcatggtc gtcaatccgg ccaccttccg cgcgccgccg
     1861 gaataatagg acgccgtccc cgttcttccg gcctgcacga gcttgtagat ctcttccgtg
     1921 ccgggatggt cctgaagacg tacccggagg acgtactcct tcttcgggtg ggccagcacg
     1981 agcccttcgg agttcaccag gtaggcgtac cccgtcttgc cggtcctgac ctgcgatact
     2041 atggccccga ggaagttcgg atcgaaaggt atgcagacga ccccgtggaa ctcgttgtcc
     2101 tcccggatcg gggcggatac gataacgatg gggtctcccg gcgttgaagg gcccctggcg
     2161 ggcacaggac cggtaacgct cgttttgccc tccctcgcgc gcaggaaata gtcgcggtcc
     2221 gagaggttaa cgcccatctg cctgggaaat tgcgcgtcaa ccctgacgat accttttctg
     2281 tcgaggacga agagcgtgaa gaaaggcctt cctatgcgct tgtaaagggc ctccagtttc
     2341 gcctggacgt gacggtagtc tccgcttcgg gaggccctga ccaccgtcgg gtccgcggca
     2401 atggatgagg ccagct
//
LOCUS       DAOQTR010000362         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_53680_length_2413_cov_4.077608, whole genome shotgun sequence.
ACCESSION   DAOQTR010000362 DAOQTR010000000
VERSION     DAOQTR010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53680_length_2413_cov_4.077608"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKN85_08595"
     CDS             complement(<1..1074)
                     /locus_tag="PKN85_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT44508.1"
                     /translation="MVSAGTKEPIITVGVADRQSETTGRLNGPYLIEGARSLSGSFHA
                     RAAASGISLFDEGGQLIADSPSVRLTAREGSTFTIFGVTIGIRFHWERKEDQTFQGNL
                     VLAARGDGTMAVINEIPVEDYLVSVISSEMSGRAPGEFLKAHAIMSRSWLIAALGKKG
                     APGAAMKPGQAPGEFVRWYNREDHDIFDVCADDHCQRYQGITKIISGEAERAVGETRG
                     LVLFHGGTVCDARYYKACGGRTENYETAWEDVHIPYLVSVTDWPDTHDPVLSEEEATR
                     WILSCPDAYCNTKDMTLLAEVLPGFDQETTNFFRWKVEYSRAELEEIIRERSETDIGT
                     LVDLVPLERGPSGRIFRLRITGTK"
     gene            1325..1627
                     /locus_tag="PKN85_08600"
     CDS             1325..1627
                     /locus_tag="PKN85_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44509.1"
                     /translation="MKRTILVVLVIAVLMIAAMPAVSDARGRHHHHGPHYRGSCCGGA
                     GWGLVGVTSGLIAGTIIGSAMTRPAPVYVVSPPPPPPPPRVYYYYPPPRRVYVYPY"
     gene            1874..2332
                     /locus_tag="PKN85_08605"
     CDS             1874..2332
                     /locus_tag="PKN85_08605"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNT44510.1"
                     /translation="MKTRFVMFSAAVILTVLVLASSSSALCVRVSKANLRSGPGKNHE
                     VAWQVYMYMPFEKVGESTDKKWYAVRDCDGDTYWIHRSLVTHRYRCAVVKVQKARVRK
                     GPGTRFSTVKDSPALKYYTYRIISSKRNWFRLRDEWGDIGWIRKDLLWIK"
BASE COUNT          474 a          695 c          729 g          515 t
ORIGIN      
        1 cttcgttccc gttatcctga ggcggaaaat gcgccccgac ggccctcttt caagaggaac
       61 gaggtcaacg agggtgccaa tgtccgtctc cgagcgctct ctgatgatct cctcgagctc
      121 cgcccgggag tattcaacct tccatcggaa gaagttcgtc gtctcctggt cgaacccggg
      181 gagcacctcc gcgagaaggg tcatgtcttt cgtgttgcag tatgcgtcag gacaagagag
      241 tatccatcgc gtcgcctcct cttcggacag gacggggtcg tgcgtatcgg gccaatccgt
      301 aacgctgaca agataaggga tatggacatc ttcccatgcg gtctcgtaat tctccgtgcg
      361 gccgccgcag gccttgtagt accgggcgtc gcagacggtg cccccgtgga agagcacgag
      421 ccctctcgtt tcccccacag cccgctccgc ctcaccggag atgatcttcg tgatgccctg
      481 gtatcgctgg caatggtcgt cggcgcagac atcgaagata tcgtggtcct ctctgttgta
      541 ccagcgaacg aactctcccg gagcctgccc gggcttcatc gccgccccgg gagcgccctt
      601 ctttccaagc gctgctatga gccagctccg ggacatgatg gcatgggcct tcaggaattc
      661 acccggtgcc cgtccgctca tctccgagga gatgacgctt acaagatagt cctcgacggg
      721 gatctcgttg atgacggcca tggtgccgtc cccccgggcg gcgagtacaa ggttgccctg
      781 aaaggtctgg tcctccttgc gctcccagtg gaagcggata ccgatggtga cgccgaagat
      841 ggtgaaggtg gacccttccc gggcggtcag ccgtacggaa ggagagtcgg caatgagctg
      901 ccctccttca tcgaagagac ttatccccga tgcggccgcc cttgcgtgaa aggaccccga
      961 cagggaacgc gcgccttcga tcaggtaagg gccgttgagg cggcccgtcg tttccgattg
     1021 cctgtccgct acacccacgg tgatgatcgg ttcttttgtg ccggctgata ccatgttttc
     1081 ccgaatgatg gtttacctta ttacgttttt gggaggttgt caatcaccgt accatgtcga
     1141 cggtaatgtc gtaactttgg ccgagcgtcg acagatcttg cctgccggcc tggtatcacc
     1201 accgctgtca tgccggaaac aacctgaatt atcggtgcgt tgcggacaaa ctccgggggt
     1261 ggtaccattc ttgctataca aaaatgctac aaagcatcac agaatctcga taggtggagg
     1321 gattatgaaa agaactatct tggtcgtcct cgttatcgcg gtcctcatga tcgccgcgat
     1381 gcctgccgtc agcgatgccc ggggcaggca tcatcatcac gggccgcatt accgtggatc
     1441 ctgttgcgga ggcgccggtt ggggccttgt gggggttaca agcgggttga tcgcggggac
     1501 catcattgga agcgccatga cccgaccggc ccccgtgtac gttgtatcgc cgccgccccc
     1561 tccacctccg ccgagggtgt actactacta tccacccccc cggagagttt acgtgtatcc
     1621 gtattagagg cggtttcagg gttccagagt tcaagggttc aagagagaaa gacgagacag
     1681 gcccggcagg ccacatgagt cctgcgggac ctgtcttcct ttttcgagac ggggagcccc
     1741 ggttgggcgg atcccgtgga acgcctcttg gcgcccggta atgttccttc ctgtaagctg
     1801 ttttaccgac gcgtccttcc cgaaaaagta gaataacagg atcacaacgc aagagtacag
     1861 ggagggactg tccatgaaga cgagattcgt catgttctcc gcagccgtca tcctaacggt
     1921 tcttgtcctc gcgtcgtcat cgtcggcgct gtgcgtcagg gtgtcgaaag cgaacctgag
     1981 atcggggccg ggaaagaacc atgaggtggc gtggcaggtc tacatgtata tgccctttga
     2041 gaaggtgggg gaatctacgg acaagaagtg gtatgccgtc cgggattgcg acggcgacac
     2101 gtactggata cacaggagcc tggtaaccca caggtaccgg tgcgccgtcg tgaaggtaca
     2161 gaaggcgcgg gtacggaagg ggccgggtac gcgcttttcc accgtcaagg acagcccggc
     2221 cctcaagtac tacacgtaca ggatcatctc ctcaaaacga aactggttcc ggctccgcga
     2281 cgagtggggc gatatcggct ggataaggaa agaccttttg tggataaagt gagaccaggt
     2341 catgggtcac gggcaatggg cgacagggga gcgcgttcct ggctttccgt aacccgtcgc
     2401 ccgttgcctg tgg
//
LOCUS       DAOQTR010000363         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_53940_length_2407_cov_2.730867, whole genome shotgun sequence.
ACCESSION   DAOQTR010000363 DAOQTR010000000
VERSION     DAOQTR010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53940_length_2407_cov_2.730867"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PKN85_08610"
     CDS             <1..616
                     /locus_tag="PKN85_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNT44511.1"
                     /translation="FVFTDFPVSTENVRLDFSQAVRDSFERITVDGECSTNDTVMLFT
                     RRGQEDADALTSFREGLRAVLKELSMMVVRDGEGATRVAHIMVEGARKKEWAEKIARR
                     IALSPLTKTAFFGADPNWGRIIAAAGDAGVPLNPSKVDITLQGEMIAKGGAEIPFSEK
                     KMKKLMSRKEISVTVDLNDGTASFDIYTTDLTYDYVKINASYRS"
     gene            638..1351
                     /gene="kdsB"
                     /locus_tag="PKN85_08615"
     CDS             638..1351
                     /gene="kdsB"
                     /locus_tag="PKN85_08615"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF009905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNT44512.1"
                     /translation="MPARYESTRLPGKPLLEIAGKPLIRLVYERASESRLQDGVIVAT
                     DDERIVQAVKAFGGDAVMTSSSCRSGTDRVFETVRQREVDLIINLQGDEPFMRPDMAD
                     LLFSVMENENLDMATLCSPIADEREYHDPNTVKVVLDDRGFALYFSRSPIPYLKNDTA
                     RPLLYKHIGIYAFKRDFLERFVKMPRSRLEEMESLEQLRVLENGFRIRVLTTQYDGFG
                     VDTPADLERARLVLDRKIV"
     gene            complement(1457..>2407)
                     /locus_tag="PKN85_08620"
     CDS             complement(1457..>2407)
                     /locus_tag="PKN85_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT44513.1"
                     /translation="LPQDGRITIKVGKKSIDFRVSTVPARFGERLCTRILDKSNTRLG
                     LDKLITHLPVRRLVEGMIRTPYGIIYVTGPTGSGKTTTLYSSLAEINDARKNIFTVED
                     PIEYDLKGINQVQVNHDIGLNFARVLRAFLRQDPDVILVGETRDAETAKIAVEAALTG
                     HLVFTTVHANDAPGTFMRLIEMGIEPFLISSSVIGVIAQRLVRKICDKCKETYHDLSA
                     TKYLGLPPDTRLYKGRGCNACGYTGYKGRVGVYEVLAVNEEIMRLVSSGAETLAVREA
                     AVNNGMIDLREYSLILLREGAISVDEVLRAVLLQNGASVH"
BASE COUNT          531 a          725 c          677 g          474 t
ORIGIN      
        1 cttcgtcttc acagacttcc ccgtttccac cgagaacgtg agacttgact tctcccaggc
       61 ggtcagggac agcttcgaac ggataaccgt ggatggagaa tgcagcacca acgacacggt
      121 catgctcttc accaggcgcg gacaggaaga cgccgacgcc ctcacaagct tcagagaggg
      181 gctccgcgcg gtcctgaaag agctttccat gatggtcgtg cgcgacggcg aaggcgctac
      241 cagggtcgcc cacatcatgg tggaaggagc gcggaagaaa gaatgggcag agaagatagc
      301 ccggcgcatc gccctgtcgc cgctgacgaa aacggccttt ttcggcgccg accccaactg
      361 gggccgcatc atagctgccg caggcgacgc cggcgtgccc ttgaaccctt cgaaagtcga
      421 catcaccctg caaggtgaga tgatcgcgaa aggcggggcg gaaatcccct tcagcgaaaa
      481 gaaaatgaag aaactgatga gcaggaagga gatatccgtc accgtcgatc tcaacgacgg
      541 cacggcctcc ttcgacatct acacaacgga tctcacatac gattacgtaa agataaatgc
      601 ttcgtacagg agctagaggc tgagaaagct catcgtcata cccgcgcggt acgaatcgac
      661 acggcttccg gggaaacccc tcctcgagat agcgggcaaa ccgctcatac gccttgtcta
      721 tgaacgcgcc tcggaatccc ggctccagga cggggtgatc gtggctaccg atgacgagcg
      781 catagtccaa gccgtaaagg ccttcggcgg tgacgcggtc atgacgagct cctcctgcag
      841 gagcggcact gacagggtct ttgagaccgt gagacagaga gaggtggatc tcatcatcaa
      901 ccttcagggc gacgagccct tcatgcgccc cgacatggcg gatctcctct tctccgtgat
      961 ggaaaacgag aaccttgaca tggccaccct gtgctcaccc attgccgacg aaagggagta
     1021 tcacgacccc aacaccgtga aggtcgtcct cgacgaccgg ggttttgccc tctatttctc
     1081 ccggtcaccc atcccttacc tgaagaacga cacggcaaga cccctgctct acaagcatat
     1141 cggcatctat gcgttcaaac gggacttcct ggaacgtttc gtgaaaatgc cgaggagcag
     1201 gctcgaagag atggaatccc tcgagcagct tcgcgtcctg gagaacggct tcaggatacg
     1261 tgtcctcacc acccagtacg acgggttcgg cgtggacacc cccgccgatc tcgagcgggc
     1321 caggctcgtc ctcgacagaa agatcgtgtg acacccgccg ccgacctgtc accatccttc
     1381 cgggagcggc cggcacgcgc cggatatgcc cttcctcccc gcgggcgaac gcgcgggcat
     1441 tatcgcatcg ccacacctaa tgcacggacg ctccgttttg caggagaacc gccctgagga
     1501 cctcgtcaac actgatggcc ccttccctga ggaggatgag gctatactct ctgaggtcta
     1561 tcatgccgtt gtttacggcc gcttccctca cggcaagcgt ttccgctcca gaggatacca
     1621 ggcgcattat ctcctcgttg accgcgagaa cctcatagac accgacacgt cccttgtagc
     1681 cggtatagcc gcaggcgttg caacccctcc ctttgtacaa cctggtatcc ggcggcaggc
     1741 ccaggtattt agtggcggac aggtcgtggt aggtctcttt gcatttgtca cagatctttc
     1801 gcaccaacct ctgggcaatg accccgatga ccgaggagga gatgagaaac ggctcgatgc
     1861 ccatctcgat aaggcgcatg aaggtacccg gggcatcgtt cgcatggacg gtggtaaaga
     1921 caaggtggcc ggtaagagct gcttcaacgg ctattttggc tgtttcagca tccctcgttt
     1981 caccgacgag gatcacgtcg gggtcctggc ggaggaaagc cctgagaacc cgggcaaagt
     2041 tgaggccgat atcatggttc acctgcacct ggttgatccc cttcaggtca tattcgatgg
     2101 gatcctcgac ggtgaagatg ttctttctgg cgtcattgat ctccgcgaga gagctataca
     2161 gggtcgtcgt cttgcctgaa cctgtgggac ctgtgacata gatgatcccg tatggcgttc
     2221 tgatcatccc ctctacaaga cggcggaccg gaaggtgcgt gataagcttg tcaagaccga
     2281 gccgcgtatt actcttgtcc aggatcctcg tgcacaacct ttcgccgaat ctggccggaa
     2341 cggtggatac acggaagtcg atggacttct ttcccacctt tatggtgatc ctcccgtcct
     2401 gagggag
//
LOCUS       DAOQTR010000364         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_53978_length_2406_cov_3.001701, whole genome shotgun sequence.
ACCESSION   DAOQTR010000364 DAOQTR010000000
VERSION     DAOQTR010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53978_length_2406_cov_3.001701"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1915)
                     /locus_tag="PKN85_08625"
     CDS             complement(80..1915)
                     /locus_tag="PKN85_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HNT44514.1"
                     /translation="MPNAQTLQTLSDERLLMALAQEQARRRKYNYIDTLYPEDGPLSR
                     HNYPKHMAFFRAGAIHNERAFLAANRIGKCLPLDAPVMMADGSWCQLSEIREGDYVMG
                     YDLATGRASPVRVTEVTRSGKMEVFETTFSDGGTVRCTREHWMPGITRSGRRQNSHGK
                     KVPVLPKKRRFYEYMRSTGNAVSSRHRFLSPNLVDYLSVGTLPLHPYVVGALLGDGSL
                     NREGVMFTTSSDEPILEKILSLTQGLVEKAVHHKGDRCCYTLYQVKDGRNGLLSNALR
                     GIGLAGQTCENKHIPDAYLTASEDDRLELLAGLVDTDGTHNDFIQKSERLTKDFARLV
                     RSLGGKATVTHEKKICTNATGGPKEAWYWRCTWRLNRRLPLVLDRKQPRISKRSVDYS
                     QRIIRKIESLGMMDCGCITVDHPDHCFIAYDWVAVGNTEGVGGYELTLHATGLYTPWW
                     EGQRFDRPIKAWAAGDTNQTVRDIIQAKLLGPIDDIGSGLIPKHLIHRTRPKQGVPDG
                     VEIIYVRHVPTGGISRIGLKSYEAGRKSFQGTEQDIIWLDEEPPLAIYAECLIRTMTT
                     KGMLLCTFTPLEGMTDVCMLFLKDGKIPEGGVAVNESTPIQIEAA"
     gene            complement(1827..>2406)
                     /locus_tag="PKN85_08630"
     CDS             complement(1827..>2406)
                     /locus_tag="PKN85_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44515.1"
                     /translation="EVAGYRGRQKNSNRSLKYKDKHPMESVIKRLMGNPRVLAALEGF
                     KMEELIGDEFGPDHIKRQLIRIAQANMLDFAKWSEDGEVKFIDSAKLTRAQGECIISV
                     SQTVTKYGGSIKIERINPLDALDKLSRIHGMYKDSLEITDPVEKELREMSDEEIRDGI
                     RSILAQRPDLADALRRAVADGLGTGAGEAKKV"
BASE COUNT          452 a          734 c          648 g          572 t
ORIGIN      
        1 cggttcagat gataaggctg tatggtgtcc tgaagggcaa caattgacat cctcccctcc
       61 gtaaaggtct ttgttctccc taagccgcct ctatctgaat gggagtgctc tcgttgactg
      121 ccacaccgcc ctcggggatc ttcccgtcct tgaggaacag catgcagaca tcggtcatgc
      181 cctcgagcgg ggtgaacgta cagagcagca ttcccttggt ggtcatggtt cggatgaggc
      241 actccgcgta gatcgccaga ggcggctcct catcgagcca gatgatgtcc tgctcggtgc
      301 cctgaaatga tttacgcccc gcctcatagc tcttgaggcc tattcttgat atcccgcccg
      361 tgggaacatg ccttacatag atgatctcga cgccgtcagg cacgccctgt ttcgggcgcg
      421 tcctatggat aaggtgcttg ggtatgagcc cgctgccgat atcgtcaatg gggcccagga
      481 gcttggcctg gatgatgtcc cggacggtct gattcgtatc gccggcagcc caggccttta
      541 tcggcctgtc gaaacgctgt ccttcccacc acggagtata cagcccggta gcatggaggg
      601 tgagctcgta tccgcccacg ccttcggtgt tccccacggc cacccaatca taggcaataa
      661 agcagtggtc cggatgatca actgttatac acccgcagtc catcatgccc aggctctcga
      721 tctttcggat gatcctctgc gagtaatcga cggatctctt cgagatccgg ggctgtttcc
      781 gatcgagaac aaggggaagc ctccgattga gccgccaggt acatcgccag taccacgcct
      841 ccttcggacc acccgtcgca ttggtgcaga tctttttctc gtgggtgacg gtggccttcc
      901 cccccaggct tcttaccagc cgggcaaagt ccttcgtgag ccgctcagat ttctggatga
      961 agtcattatg tgttccgtca gtatcaacca ggccggcgag cagctcaagg cgatcgtcct
     1021 cgctggccgt aaggtaggcg tcaggaatgt gcttgttctc gcatgtctgt cctgccagcc
     1081 cgatgcccct gagggcattg ctcaagagac cattccttcc gtctttgacc tggtacagcg
     1141 tgtagcagca ccgatcaccc ttatggtgaa cagccttttc gacaaggcct tgcgtgagcg
     1201 atagtatctt ctcaaggatg ggctcgtcag aggaggtagt aaacataaca ccctctcggt
     1261 tgagcgatcc gtcaccgagg agagccccaa caacgtaggg atgaagcggt agcgttccga
     1321 ccgagagata atcaaccaga ttcggggaaa gaaaccggtg cctggatgaa acagcattgc
     1381 cggtggatct catgtattca tagaatcggc gcttcttcgg cagaacagga accttcttgc
     1441 catgagagtt ctggcgtctt ccgctcctgg tgatcccggg catccagtgc tccctggtgc
     1501 accgcacggt cccgccgtca gagaacgtgg tttcaaagac ctccattttc ccggagcggg
     1561 tgacctctgt cactctcaca ggagaagccc tgccggtggc aaggtcgtac cccatgacat
     1621 agtcgccctc tcgaatctcc gagagctggc accacgatcc atccgccatc atcaccggtg
     1681 cgtccagcgg caagcacttt ccgattctgt tcgcagccag gaacgcccgc tcgttgtgta
     1741 tggcgccggc ccggaagaag gccatgtgct tggggtagtt atgccgcgag agaggcccgt
     1801 cttccggata gagtgtatcg atgtagttat actttcttcg cctcgcctgc tcctgtgcca
     1861 aggccatcag caaccgctcg tctgagagcg tctgcaaggt ctgggcgttg ggcaaggata
     1921 gagcggatac cgtctctgat ttcttcatca ctcatctcgc gcaattcttt ttcgaccggg
     1981 tctgtgatct ccaggctgtc cttatacatg ccgtgtatcc gggacagctt atccagtgcg
     2041 tccagtggat ttatccgttc gatctttatg ctgccgccgt acttcgtcac ggtctgggac
     2101 acgctgatga tgcactcccc ctgcgcccgg gtcagcttcg cggaatcgat gaacttgacc
     2161 tcgccatcct cgctccactt cgcaaagtcc agcatgttcg cctgggcgat ccggatcagc
     2221 tgcctcttga tgtgatcagg cccgaactca tccccgataa gctcctccat cttgaagcct
     2281 tcgagcgccg ccagcaccct ggggttcccc atgaggcgct tgatcacgct ctccatgggg
     2341 tgcttgtcct tatacttcag gcttcggttc gagttctttt gcctccccct gtagccggcc
     2401 acctcc
//
LOCUS       DAOQTR010000365         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_54127_length_2401_cov_4.456948, whole genome shotgun sequence.
ACCESSION   DAOQTR010000365 DAOQTR010000000
VERSION     DAOQTR010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54127_length_2401_cov_4.456948"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKN85_08635"
     CDS             complement(<1..353)
                     /locus_tag="PKN85_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNT44516.1"
                     /translation="MIFSVLTLFPGIFASPLKESILGKAAAKGLVGFNIINIRDFADD
                     PHRSCDDTPYGGGPGMVMKVEPIFRAIEHVKEVHGRARFVLLSPQGRSFDQFTAERFA
                     RSSHICLICGRYEGV"
     gene            complement(350..847)
                     /gene="rimM"
                     /locus_tag="PKN85_08640"
     CDS             complement(350..847)
                     /gene="rimM"
                     /locus_tag="PKN85_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNT44517.1"
                     /translation="MKWIPVGRVVSTFGIRGELKFHYYNEVKEELLDYTSLFALKGSE
                     FLELKPEGARYRKRLFYLSFQGFSTVEEVSFLVGKELFVREEDLPRLEEGEYYEYQLI
                     GLEVTTVQGSRLGKVRSLMHTGASDILVVEGESELMIPMVEGFIIDIDVSGGTVRVDV
                     EGLIP"
     gene            complement(847..1080)
                     /locus_tag="PKN85_08645"
     CDS             complement(847..1080)
                     /locus_tag="PKN85_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNT44518.1"
                     /translation="MLKELIEYIAKALVDNPDLVRVSEIEGEKTSVIELNVAKDDLGK
                     VIGKQGRTARAMRTILSAASTKVKKRAVLEIIE"
     gene            complement(1099..1347)
                     /gene="rpsP"
                     /locus_tag="PKN85_08650"
     CDS             complement(1099..1347)
                     /gene="rpsP"
                     /locus_tag="PKN85_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNT44519.1"
                     /translation="MKFRLSRFGAKKKPFYRIVVADERSPRDGRFIDKVGFYDPLKNP
                     AEISLDKEKIKTWYEKGVRPTRTVESLFKREGVLKELS"
     gene            complement(1365..>2401)
                     /locus_tag="PKN85_08655"
     CDS             complement(1365..>2401)
                     /locus_tag="PKN85_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNT44520.1"
                     /translation="AGLPAAICRSSSGKTTTCAKLAIHIRKMGRKPLLVPSDVYRPAA
                     IEQLVKLGKQINVEAFTMDVVKDPVTICKEAKIYAMRNGYDTVIVDTAGRLHIDDAMV
                     NELVEQKKVLNPKETLLVLDAMTGQDAVSIAGVFNEKLGVDGIILTKLDGDTRGGAAI
                     SIKAVTGKPIKFIGVGEKLDALEPFFPDRLASRILGMGDVVSLVEKAQDVFDEKEARE
                     LERKFRKDEFTLEDFRDQIRQMKKLGSLESIIGMIPGMNKFKGAINFAEAEKDIKKTE
                     AIINSMTSKERARPNIIDGSRRLRISKGSGTQVQDVNDLLRKYMETKKMLKKFTKGGA
                     KGLQRQLFSR"
BASE COUNT          475 a          738 c          598 g          590 t
ORIGIN      
        1 tcgacgccct cgtatcggcc gcagatgaga caaatgtggg acgaccgggc gaagcgctcg
       61 gccgtgaact ggtcgaaact tctgccctgc ggcgaaagga ggacgaacct ggccctcccg
      121 tggacctcct ttacgtgctc tatggccctg aagatgggtt caaccttcat gaccatcccc
      181 ggcccaccgc cgtagggcgt gtcgtcgcag gagcggtgag ggtcatcggc aaaatcgcgt
      241 atgttgatga tattgaaccc gacaaggccc ttggcggccg ccttgccgag tatgctctcc
      301 ttgaggggcg acgcgaatat gcccgggaac aatgtgagga ccgagaagat cacgggatga
      361 gcccttcaac gtccacgcgg actgtaccgc cgctcacgtc tatatcgatg atgaatcctt
      421 ccaccatggg gatcatcagt tcactttctc cttcgacgac aaggatgtca ctggcacccg
      481 tgtgcatgag cgaccggacc tttccaagac gcgacccctg cacggtcgtc acctcaagac
      541 cgatcaattg gtactcgtaa tactcgcctt cctccaaccg cggaaggtct tcctcccgga
      601 caaaaagctc cttccccacc aggaatgaga cctcttcgac cgttgagaac ccctggaagg
      661 aaagatagaa caagcgtttc ctgtatcttg ccccttccgg cttaagttcc aggaactcac
      721 tgcctttaag ggcaaacaac gaggtgtagt cgagtaattc ttccttcacc tcgttgtaat
      781 agtgaaactt gagctcaccc cgtatgccga atgtcgacac gacacggccg acagggatcc
      841 acttcattat tcgatgatct cgaggaccgc tctcttcttc accttcgtcg aagccgcgct
      901 caggatcgtc ctcattgccc tggctgttct gccctgtttc ccgatgacct tcccgaggtc
      961 atccttcgca acattgagct caatgaccga cgtcttctcg ccttcgatct cggagaccct
     1021 gaccagatca ggattgtcaa ctaacgcctt cgcaatgtac tcgatcaact ctttcaacac
     1081 gatccacctc cgttggtttt atgaaagctc cttaagaacc ccctctctct tgaacagact
     1141 ttcaacggtc ctcgtcggcc gtacaccttt ttcgtaccac gttttgatct tttccttgtc
     1201 gaggcttatc tccgccgggt tcttcaacgg atcgtagaac ccgaccttat cgatgaacct
     1261 cccgtccctg ggggaacgct catcggccac aacaatgcgg taaaaaggtt tcttctttgc
     1321 tccaaacctc gacagtctaa atttcacagc caaaatccca cctcctatcg tgagaaaagt
     1381 tgtctctgga ggcccttcgc gcctcccttc gtgaacttct tcagcatctt ctttgtttcc
     1441 atgtattttc tcaaaaggtc gttcacatcc tggacctggg tcccgctgcc cttcgagatg
     1501 cgcagccttc ggctgccgtc tatgatattg ggacgggccc tttccttcga ggtcatggaa
     1561 ttgatgatcg cttccgtctt ctttatgtcc ttttcggcct cagcgaagtt gatggcccct
     1621 ttgaacttgt tcatgccggg gatcattccg atgatcgatt cgagggaacc cagtttcttc
     1681 atctgtcgta tctggtcacg gaaatcctcg agggtgaatt cgtccttgcg gaacttccgc
     1741 tccagctccc gggcctcttt ctcgtcgaag acatcctgtg ccttctccac aagggaaacg
     1801 acatccccca tgccgaggat ccgcgacgcc agtctgtcgg ggaaaaacgg ttccagcgca
     1861 tccagtttct cgccgacacc gatgaacttg atgggcttgc ccgtcaccgc cttgatggat
     1921 atggcggcgc cgccccgggt atcgccgtcg agcttggtga ggattattcc gtccacgccg
     1981 agcttctcat tgaagacccc ggctatgctc accgcgtcct gaccggtcat ggcgtccagg
     2041 acgagaaggg tctccttcgg gttcaagact tttttctgtt cgacgagctc gttcaccatg
     2101 gcgtcgtcga tatgcagtcg tccggcggtg tcgacgatca ccgtgtcgta gccattccgc
     2161 atggcgtaga tcttcgcctc tttacagatc gtcacggggt ccttgacaac atccatggtg
     2221 aaggcctcaa cgttgatctg cttgccgagc ttgacgagct gctcgatggc ggccggccgg
     2281 taaacgtctg aaggaacgag aaggggcttg cgtcccatct tacggatatg gatggcgagc
     2341 ttcgcgcacg tcgtcgtctt gcccgagctc gaacgacaga tcgctgccgg cagaccggct
     2401 g
//
LOCUS       DAOQTR010000366         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_54272_length_2397_cov_3.286934, whole genome shotgun sequence.
ACCESSION   DAOQTR010000366 DAOQTR010000000
VERSION     DAOQTR010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54272_length_2397_cov_3.286934"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..>2397)
                     /locus_tag="PKN85_08660"
     CDS             complement(171..>2397)
                     /locus_tag="PKN85_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNT44521.1"
                     /translation="NRALYIRQITQMQRPGAIAFNQVLLADVYHHVGREAEAESLLED
                     ALNGWDKDQDLPPEAIAFSLTLIAKIYRTSGRYAEAEPLIQRSLAICEKGLGPDHPTV
                     AFALNNLAMIYRRTGRNADVESLYKRSLAIFRKSFGDNHPAVSASLNNLAFYYGTTGG
                     HQQAHSCFTSSLGIQDSLRESVFTLLTEKQKLSYMKTQEMTIHGYVSHTLSFPRSSAA
                     TVVDAFNAWLRWKGSVIEAQGRYLDALGASDDPKVRETFKELTALKRDIARLQLAGTG
                     KTAPGDYARKMSELVKKKESVEPELMAASREFMLQKKAQTADVGTLSSALSRDSAYLD
                     YARITLFDFRKRTWTEPHYLLFLLTPGETVEVQLIDTGASENVDRHINAYLGEMARAG
                     REGVLPSEKKLKAEASVLYDILVKPVEPSLKGRKRLLVSPDGNLNLIPFEALVEPAGT
                     YLIEDFLVSYVGAGRDMLKFAKAKLAGGESLIIADPDYDLGQKEVEKARSSAGLRSAG
                     TKGHVSRDAAGMRFDRLPDTREEADTIEKILHDRFGQKVFNEQDKRALELVLFRVSSP
                     KVLHLATHGYFLAEEETKQSEKAAELPLTGDPLQDTPITIENPMLRSGIVLAGANASL
                     KEGKDEGMVTAEKVLGLNLGGTDLVVLSACQTGVGDIQSGEGVFGLKRAFILSGAKTL
                     VMSLWSVPSKETVDLMTAFYALLSEGKTPSEALSQAKLTLMKQKPHPFFWAAFVLTGS
                     P"
BASE COUNT          441 a          682 c          734 g          540 t
ORIGIN      
        1 tttctccacc gagcgggatc atgccgtgag acagagttta tcaacaggct gttgaaggtt
       61 aaaaaccgaa gataaagaga cagacacggc caggcctagg aagccttccg gatctggccg
      121 tactctttcg tctctcactc tcaagcgatt caagcaattt gaacgggttc ctagggtgag
      181 cctgtcagga cgaaggctgc ccagaagaag gggtggggct tttgcttcat gagggtgagc
      241 tttgcctggc tgagcgcttc tgacggggtc ttcccctcgg aaaggagggc gtagaaggcg
      301 gtcatcaggt ccaccgtttc ttttgaggga acactccaga gactcatgac gagggtcttt
      361 gccccggaaa ggatgaaggc ccttttcaac ccgaagacgc cttcgccgct ctggatgtcg
      421 ccgacgcctg tctggcaggc ggacaggacg acgaggtcgg tgcccccgag gttgagcccg
      481 aggaccttct cggccgtgac catgccctcg tcctttccct ccttgagaga cgcgttggcc
      541 ccggcaagga ctatacccga gcgcagcatc gggttctcta tggtaattgg agtgtcctgc
      601 agcgggtcgc ccgtcaacgg gagctctgcc gccttctcgg actgctttgt ctcctcttcg
      661 gcgaggaagt acccgtgcgt ggccaggtga aggacctttg gggaggacac gcggaacagg
      721 acaagctcca gcgccctctt gtcctgttcg ttgaacacct tctgcccgaa cctgtcatgc
      781 aggatctttt caatggtgtc ggcctcttcc ctggtgtcgg gaagacggtc gaaacgcatg
      841 cctgccgcgt ccctcgagac atggcctttc gtgcccgccg acctgagacc ggccgatgac
      901 cgcgccttct cgacctcctt ctgtcccaga tcgtagtcgg ggtctgctat tatgagcgat
      961 tcaccgcctg caagctttgc cttcgcgaat ttgagcatgt ctctgcccgc tccaacgtaa
     1021 ctgaccagga aatcttcgat gagataggtg cctgccggct ccacaagggc ctcaaagggg
     1081 atgaggttga gattgccgtc gggactaacg agaaggcgct ttctcccttt cagggaaggt
     1141 tcgacgggct tgacgagaat gtcatagagg accgatgcct cggctttaag cttcttttcg
     1201 ctcggcagca cgccttccct tccggcacgt gccatctccc cgaggtacgc gtttatgtgc
     1261 ctgtccacgt tttcgctcgc ccccgtgtcg atgagctgta cctccacggt ctcccccggg
     1321 gtgaggagga agagcaggta atggggttcc gtccatgttc tcttcctgaa atcgaagagg
     1381 gtgatccttg cgtagtcgag ataggcagag tcccgggaaa gggctgatga aagggtcccg
     1441 acatccgccg tctgcgcctt cttttgcagc atgaactccc tgctcgcggc catgagctca
     1501 ggttcgacag attccttctt tttcaccagc tcggacatct tcctcgcgta atccccgggc
     1561 gccgtctttc ccgtcccggc aagctggagc cttgcgatat cccgcttcag cgccgtcagt
     1621 tccttgaacg tctcccgcac cttgggatcg tcggacgcgc ccagggcgtc gaggtaacgc
     1681 ccctgggcct ctatgacgga gcccttccac ctaagccagg cattgaaggc atcgactacg
     1741 gttgcggcgc tgctgcgggg aaaactcaag gtgtggctca cgtatccatg gatcgtcatc
     1801 tcctgggttt tcatatagga gagtttttgc ttttccgtga gaagtgtgaa gacggattcc
     1861 cggagcgaat cctgtatgcc aagggagctg gtgaaacagg aatgggcctg ctgatgtccg
     1921 cccgtcgtcc cgtagtagaa ggcaagattg ttgagcgatg cgctgacggc aggatggttg
     1981 tccccgaagc tcttccggaa tatcgcgagg gagcgcttgt acagggattc cacatcggca
     2041 ttccggccgg tgcgtcgata gatcatggcg aggttgttga gggcgaaggc gacggtggga
     2101 tggtccgggc caagcccctt ctcacatatc gcgagggagc gttggataag tggttccgcc
     2161 tcggcgtaac ggccgctcgt gcggtagatc tttgctatca gggtgaggga gaaggcgatc
     2221 gcctcgggag gaaggtcctg gtccttgtcc cagccgttaa gcgcatcctc taacagggac
     2281 tccgcctcgg cctcacggcc aacgtggtgg tagacatcgg cgagaagcac ctggttgaag
     2341 gcgatggcgc cggggcgctg catctgtgtt atttggcgga tgtagagagc gcgattg
//
LOCUS       DAOQTR010000367         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_54385_length_2394_cov_3.532279, whole genome shotgun sequence.
ACCESSION   DAOQTR010000367 DAOQTR010000000
VERSION     DAOQTR010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54385_length_2394_cov_3.532279"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKN85_08665"
     CDS             complement(<1..211)
                     /locus_tag="PKN85_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44522.1"
                     /translation="MGTERSYHLSSRIQLIGILFLLFVVDGLALWFTARFDYTRTLEH
                     AKTILQKTTISLEERVRRTIDSTEAI"
     gene            508..981
                     /locus_tag="PKN85_08670"
     CDS             508..981
                     /locus_tag="PKN85_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT44523.1"
                     /translation="MEKESKELMRSESGRMMHPLWDMERWLERNFMRPLSLFHPMMTR
                     SLTEELMPPVDILEENSEVVIRMEVPGVKKEDIDVTLTGNTITISGERKTDKEVKRKD
                     YYLAEHSYGSFCRSFGLPSEVQADKVKSTMKDGILEVRLPKTEEAKKKEIHVKVQ"
     gene            complement(1092..1394)
                     /locus_tag="PKN85_08675"
     CDS             complement(1092..1394)
                     /locus_tag="PKN85_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT44524.1"
                     /translation="MPEFGSPFSGLANDRKLTEQELIRAIRFMIAAEYEAVQLYMQLA
                     ESTDNRLAIKVLRDIADEERVHAGEFLRLLRELDPDEEKFYAEGAEEVEEEIGKLK"
     gene            complement(1505..>2394)
                     /gene="ablB"
                     /locus_tag="PKN85_08680"
     CDS             complement(1505..>2394)
                     /gene="ablB"
                     /locus_tag="PKN85_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative beta-lysine N-acetyltransferase"
                     /protein_id="HNT44525.1"
                     /translation="FPQTMSGTADGRTMLDRPVDTLEHFEGCLIQHGPSNDRVYLMKL
                     GPSDPERVALSLIAKATEQGYSKVFAKVPDSAQDAFLRHGFQVEARIPGFYNGSKDAA
                     FLGFYLSYERTREENRSVLDSLLDLYLNDSASPPAALPDGFLLRLCSRDDTSRMAQIY
                     STVFPSYPFPIHDPSYLRETMETHVRYFGIEAVGTLIALSSAEMDRECGNVEMTDFAT
                     LPEWRGYRLSVHLLRAMEEAMRAERFITAYTIARSSSPGMNLTFARCGYRYGGRMINN
                     TNISGQIESMNVWYKRLED"
BASE COUNT          517 a          634 c          699 g          544 t
ORIGIN      
        1 ggatcgcctc cgttgagtcg atcgtccttc tcaccctttc ctccaatgag atagtggtct
       61 tttgaaggat ggttttcgcg tgctcgaggg tcctcgtgta gtcgaatctc gccgtgaacc
      121 agagggcaag gccgtcgacg acgaagagaa gaaaaaggat gccgatcagc tggatcctgg
      181 aagaaagatg atatgatcgt tctgttccca ccctggagcc ccttggggtt tggtaatgac
      241 cggccggtga tagcttcctt ctatactgat tatcatgcct tgctcgatat gccaacctct
      301 gatggtgccg gaggcgctca tcgcctgaca ggcggccttc caggcgtgtc attgaaaggg
      361 atgacatgtc cgttgtcgaa aaaccgtcga agaattgtcc tttctcctac agaccggagg
      421 agcttccctg attatcctgt tattgtagac caacaacctt ggtattaccg ggtagaaagg
      481 ctttttaggc aaaaatggag gtgtgccatg gaaaaggagt caaaggaatt gatgaggtcc
      541 gaatccggca gaatgatgca tcctctctgg gacatggaaa gatggctgga aaggaatttc
      601 atgcggccct tgtccctctt tcacccgatg atgaccagat cactgacgga ggagttgatg
      661 cctcccgtgg atatactgga ggagaacagc gaggttgtga tcaggatgga ggtgccgggt
      721 gtgaagaagg aggatatcga tgttacgctc accgggaaca cgatcaccat ttcgggggaa
      781 cggaaaaccg acaaagaggt aaagaggaag gactattatc tcgccgagca ctcctatggt
      841 tccttctgcc gctccttcgg tctcccttcg gaggttcagg cggacaaggt caaatcgacc
      901 atgaaggacg gcatcctcga ggtgaggctc ccgaagaccg aggaggcgaa gaagaaggag
      961 atacacgtca aggtccagta gtgttgcggt aagtcctcac aacccccata aaccgcagtc
     1021 cggcaagagg ctcctgatgg aacgcattcc gtcggactgc ggcacctttc caaaaaccgt
     1081 ttccgagggg gctatttcag cttcccgatc tcttcttcga cctcttccgc cccttctgcg
     1141 tagaacttct cttcatcggg gtccaattcg cggagcagac gcagaaactc gccggcatga
     1201 acacgttctt cgtcggctat gtcacgaagg acctttatgg cgagccggtt gtccgtcgac
     1261 tccgcgagct gcatgtagag ctgtaccgcc tcgtattcgg cggcgatcat gaaccggatg
     1321 gcccggatga gctcctgctc cgtgagtttg cgatcgtttg ccagtcccga gaaaggtgat
     1381 ccgaattccg gcatggcgat cctcccttag ttttgttttt gcatggccgt ctgtgacctg
     1441 tacgcatact aaattgcgtt cggccgcctg tcaagactgc ccctcctgga agatcggttg
     1501 gtcttcagtc ttccagacgc ttgtaccaga cgttcatgct ctctatctgt cccgatatgt
     1561 tcgtgttgtt gatcatcctt ccaccatacc ggtagccgca tctggcaaag gtgaggttca
     1621 tccccgggga ggatgatctg gctatggtgt acgccgttat gaagcgttcg gcacgcattg
     1681 cctcttccat tgcccggaga agatgaacgg acaggcggta tcccctccac tcgggcaggg
     1741 tggcgaagtc ggtcatctcc acgtttccgc actccctgtc catttccgcg gaagacaggg
     1801 cgataagcgt tcccaccgcc tcgatcccga aatagcggac atgtgtttcc atggtttcgc
     1861 gaagatagga agggtcgtgg atggggaagg gataggacgg aaagaccgtc gagtagatct
     1921 gtgccatcct ggaggtatcg tcgcgggaac agaggcgcag gaggaagccg tcaggaagcg
     1981 ccgccggggg tgacgcggaa tcgttcaggt aaagatcgag caggctgtcg agcacggagc
     2041 ggttttcctc gcgggtccgc tcgtaggaca ggtagaaccc gagaaaggcc gcatccttag
     2101 acccgttgta aaaaccgggg atacgggctt ccacctggaa accgtgacgg aggaacgcgt
     2161 cctgcgccga atcgggcacc ttggcgaata ccttggaata cccctgttcc gttgccttcg
     2221 cgataaggct caaggccacc ctctcgggat ctgaaggtcc gagcttcatg aggtagaccc
     2281 tgtcgttcga ggggccgtgc tggatgaggc agccctcaaa atgctccagc gtgtcgacgg
     2341 gtctatcaag catcgttcga ccgtctgctg tgccgctcat tgtctgcggg aacc
//
LOCUS       DAOQTR010000368         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_54395_length_2394_cov_2.846516, whole genome shotgun sequence.
ACCESSION   DAOQTR010000368 DAOQTR010000000
VERSION     DAOQTR010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54395_length_2394_cov_2.846516"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1298)
                     /locus_tag="PKN85_08685"
     CDS             complement(21..1298)
                     /locus_tag="PKN85_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNT44526.1"
                     /translation="MNKRPFVVIAVAVILILAAAAAVRSFFWKPSDRSEVRVIPEGEY
                     DPAVWGRHYPLEYGSFQKNQEMTASPTDFGGSMKFQHSLRQPEILTNFKGMAFSKDYT
                     EDRGHPHALTDLKESGRITLQSPGACMTCKSAHIIDIYRSSGEAYARKPLGELFAKMK
                     YSITCANCHDPATMSLRVSNPAFIEAMRKKGIDVAKAPREEMRSYVCGQCHAEYYFEP
                     ETSRVVLPWDKGLHPEQVYAFYGGKPGGFEGDWVHPDSQTAMLKAQHPDFEIFSAGTH
                     ARAGISCADCHMPYMRERGRKYSSHWVTSPMKHIDASCRRCHPEEAKWILSRVKATQK
                     NVWELQHTAGTTVAKAHEAVARAASLNGTNRAELDRAKEYLRKAQWYWDFVAAENSMG
                     FHNPALCLNILGQSIDLAHKAREAAGKAAGIAP"
     gene            complement(1291..1788)
                     /locus_tag="PKN85_08690"
     CDS             complement(1291..1788)
                     /locus_tag="PKN85_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNT44527.1"
                     /translation="MPRRNEDSADFDAVIKRHVWRLVGMGACLGAIVVVVIFSGYHLG
                     GFSYFCGSCHSMENNYFAWKTSRHKQFACIECHMPRDNLAYAVAYKIYAGIRDLAGET
                     MRTYPFTIKLTGHARAIANSNCLRCHFSTVEKTPMARGNPECMKCHRFLVHGRPVEQG
                     GNRLE"
     gene            1955..>2394
                     /locus_tag="PKN85_08695"
     CDS             1955..>2394
                     /locus_tag="PKN85_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNT44528.1"
                     /translation="METFWITFTVAVMLAGLAGTFLPMLPGVPLIYACLIFYGFVSGW
                     KAYGLVTVIVWGVVTGVTVLIDLYAGSVGAKRYGASRYGFWGAVLGAVFGAVVAGLPG
                     IIIGPIIGAVAGELLAGRSYREAWLSGWGTFIGFLSGSLVKIIVA"
BASE COUNT          401 a          692 c          726 g          575 t
ORIGIN      
        1 ggagttgctc caaattcctg tcacggggcg atgcccgcag cctttccggc tgcctccctg
       61 gccttgtggg ccagatcgat ggactggccg aggatgttga ggcacagggc cgggttatga
      121 aaacccatgc tgttctcggc ggcgacaaag tcccagtacc actgggcctt acgcagatac
      181 tcctttgccc tgtccagttc cgccctgttc gttccgttca aggaggctgc cctggctacg
      241 gcctcgtgag ccttcgcgac cgttgtccca gccgtgtgct gcagctccca gacgttcttc
      301 tgcgttgcct tcacgcggct gaggatccat ttcgcctctt cggggtggca tcgccggcag
      361 gacgcgtcaa tgtgcttcat ggggcttgtc acccaatggg aactgtattt cctgccccgc
      421 tcccgcatgt acggcatgtg gcaatcggcg caggatatgc cggccctggc gtgagtgcct
      481 gccgagaata tctcgaaatc gggatgctgg gccttcagca tcgccgtctg cgaatcggga
      541 tgcacccagt cgccttcgaa cccgccgggt ttaccgccgt agaaggcgta gacctgctca
      601 gggtgcagtc ccttgtccca gggcagcacc acccgtgatg tttcaggttc aaagtagtat
      661 tcggcatggc actggccgca cacgtagctg cgcatctctt cccggggcgc cttcgcgacg
      721 tcgatgccct tttttcgcat ggcttcgatg aaggcgggat tggagacgcg aagcgacatt
      781 gtggctggat cgtggcaatt cgcgcaggtg atggagtatt tcatcttcgc gaaaagctct
      841 ccgagaggtt tcctggcgta ggcctccccg gaactcctgt agatatcgat gatgtgggcg
      901 gatttgcatg tcatgcacgc gccggggctc tggagggtga tcctcccgga ttccttgagg
      961 tcggtgaggg catgcgggtg cccccggtct tccgtgtagt ccttgctgaa ggccatgccc
     1021 ttgaaattcg tgaggatctc gggctgcctg agggagtgct ggaacttcat ggaaccgcca
     1081 aagtcagtgg gtgacgcggt catctcctgg ttcttctgga aacttccgta ctcgagggga
     1141 tagtggcggc cccagacggc gggatcgtat tccccttccg gtatgactct cacctccgac
     1201 ctgtcggaag gtttccagaa gaaggatcga accgctgccg cggccgcgag gatgaggatg
     1261 acggcgacgg caatgaccac gaaaggtctt ttattcaagc ctgttccctc cctgttccac
     1321 gggtcttccg tggacgagaa acctgtgaca cttcatgcac tcggggttgc ccctcgccat
     1381 gggcgtcttc tcgacagtcg agaaatgaca cctcaggcag ttggagttcg ctatcgcgcg
     1441 cgcgtgtcct gtgagcttga tcgtgaaggg atacgttcgc atcgtctctc ccgccaggtc
     1501 gcggattccc gcgtagatct tgtaggcgac ggcgtaggcc agattgtccc ggggcatgtg
     1561 acactcgata caggcgaatt gcttgtgtct cgatgttttc cacgcgaaat aattgttctc
     1621 catgctgtgg caggagccgc aaaagtacga aaaccctccg agatgatagc cggagaagat
     1681 gaccaccacg acgatggccc cgagacaggc ccccattccg acgagtctcc agacgtgtct
     1741 ctttatgacg gcatcaaaat cggcgctgtc ctcgtttctc cggggcatcc tttttcccct
     1801 tgaccctggt cttggaacat gaacagttta ttatggaggt gtatccccgt caacccaaaa
     1861 gaccgtttca ggttcggggt ttcaagtcta aggcaaaaga ccgttctttc ttccgggact
     1921 tgaaaccccg aaccgttcag gggtaatgtt gagtatggag accttctgga tcaccttcac
     1981 cgtggctgtt atgcttgcag gtctcgcggg gacgttcctt cccatgctcc ccggggtgcc
     2041 gctcatatac gcttgcctca tcttttacgg tttcgtctcc gggtggaagg cgtacggtct
     2101 tgtcacggtc atcgtctggg gtgtggtcac aggcgtcacc gtgcttatcg acctttacgc
     2161 cgggtctgtc ggcgcgaagc gatacggcgc gtcgcgttac ggtttttggg gcgcggtgct
     2221 gggtgcggtc ttcggggccg ttgtcgccgg cctgccgggc atcattatcg gccccattat
     2281 cggggccgtc gcgggagaac tcctggccgg gcggtcctac cgcgaggcct ggctgtcggg
     2341 ttggggcact ttcatcggtt ttctgtcggg aagccttgtc aagatcatcg ttgc
//
LOCUS       DAOQTR010000369         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_55092_length_2375_cov_3.019828, whole genome shotgun sequence.
ACCESSION   DAOQTR010000369 DAOQTR010000000
VERSION     DAOQTR010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55092_length_2375_cov_3.019828"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKN85_08700"
     CDS             complement(<1..729)
                     /locus_tag="PKN85_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT44529.1"
                     /translation="MKIDLDKCKGCGLCEEVCPLGVITLKDRKAAIGPGCVECKTCMK
                     VCPEAAFTPETPADHPMCTSCPIMCRIPEGGHGACMRYYNEKGEILRVGRVHSYEEVM
                     DLVREGTGAGPIGTPLITGIGSGTTYPDFRPSPFIVSGVKDGIDIVTVVTEAPLSYSG
                     VKLKVDTDLHVGEEGKKVYVRRKGKRHIGHLCTEEYGSKILSLGGVNILTSKNGLFAA
                     RVLHEFLQGKKIKVEVEDGTKLELA"
     gene            complement(751..1896)
                     /locus_tag="PKN85_08705"
     CDS             complement(751..1896)
                     /locus_tag="PKN85_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT44530.1"
                     /translation="MSTLVIRNVGTIFTGDIERPVVNGPVSILIEDGKIKAIEGTEPK
                     ADKAIDANGMTVCPGFIDSHVHFVFGDFTPRQSQLGYIESSLHGGVTSMISAGEVHLP
                     GRPTDPKGTKALALLAQKSFAKLRPAGVKTYGGGLILEKGLVEADFKELAEDGVWLVG
                     EIGLGSIKAAEEARQMVEWAKKYGMKVLMHVGGTSVPGSSTVTCDDVLVAKPTVACHL
                     NGGPTSIPVPEVKRVIEQTDCAIEVVHCGNPLSALEIGRFMKEKGMLKRLILGNDAPS
                     GTGVIPLGIWRMVNFMSSLCDIPAETAVCFATGNTKNVFGLAQGIVAPGCDADLQIID
                     APMGSAGKDAKAAIEVGDIPGIGMVLIDGAVKTQVSRNTPPPVRKTA"
     gene            complement(1919..>2375)
                     /locus_tag="PKN85_08710"
     CDS             complement(1919..>2375)
                     /locus_tag="PKN85_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT44531.1"
                     /translation="APYATYAFATHMTQVDVDEVTGVCKVIKVHAAHDVGRAINVSSV
                     RGQIYGGVAMGIGLALMEEFIPGKSGSFDNYYLPTSMDMPEVEAMIVEDREPTGPFGA
                     KGVGEPALIPQAAAIVNAIKDATGVRMRQLPCDIERLKLALEKDKENKG"
BASE COUNT          378 a          766 c          700 g          531 t
ORIGIN      
        1 ggcgagttcc agcttcgtgc cgtcttcgac ctcgaccttt atcttcttgc cctgaaggaa
       61 ctcgtggagg accctcgcgg caaacagacc gtttttcgat gtgagtatgt tcacgccgcc
      121 gagggagagt atcttcgacc cgtattcctc ggtgcagagg tggccgatgt ggcgtttgcc
      181 ctttctgcgg acgtacactt ttttgccttc ctcccccacg tggaggtccg tgtcgacctt
      241 aagcttcacg ccgctgtagc tcaagggggc ctcggtgacg acggtgacga tgtcgatgcc
      301 gtccttgacc ccgctgacga tgaaaggcga gggtctgaag tcagggtagg tcgtacccga
      361 gccgatcccg gtgataaggg gcgtgccgat gggtccggcg cccgtccctt cgcgcacgag
      421 gtccatcacc tcttcatagg agtggacccg gcccacgcgc agtatctcac ctttttcgtt
      481 gtagtatctc atgcaggcac cgtgcccgcc ctcggggatc ctgcacatga tggggcacga
      541 ggtgcacatg ggatggtccg cgggggtttc cggcgtaaag gcggcctcgg ggcacacctt
      601 catgcaggtc ttgcactcga cacaaccggg gccgatggcg gccttcctgt ccttgagggt
      661 tatcaccccc agggggcaca cctcttcgca gagcccgcag cctttgcatt tatcaaggtc
      721 tatcttcata tgacaccccg ggcttttccc ttacgccgtt ttcctgacgg gcggcggggt
      781 gtttctgctg acctgtgttt tcaccgctcc gtcgatgagg accatcccga tgccggggat
      841 gtccccgacc tcgatggccg ccttcgcgtc cttgccggcg gaacccatgg gagcgtcgat
      901 gatctggaga tcggcgtcgc agccgggcgc cacgatgccc tgggcgagtc cgaagacgtt
      961 cttcgtgttc cccgttgcga agcagactgc cgtctcagcg ggtatgtcgc agagggagga
     1021 catgaagttg accatcctcc atatcccgag agggatgacc cccgtccccg agggggcgtc
     1081 gttgccgagg atgagccgct tcagcattcc cttttccttc atgaacctgc cgatctcgag
     1141 ggccgacagg ggattgccgc agtgcaccac ctcgatggcg cagtccgtct gctcgatgac
     1201 ccgcttcacc tccggcacgg ggatgctcgt gggtccgccg ttcaaatggc aggccaccgt
     1261 gggtttcgcc acgaggacat cgtcacaggt cacggtggag cttcccggca ccgatgtgcc
     1321 gccgacgtgc atgaggacct tcatgccgta cttctttgcc cactcgacca tctgccgcgc
     1381 ctcttcggcc gcctttatgc tcccgagacc gatctcgccg acaagccaca cgccatcttc
     1441 ggcgagttcc ttgaagtcgg cctcgacgag acctttttcg agtatcaggc cgccgccgta
     1501 tgtcttaacg cccgccggcc tcagcttggc gaaggatttc tgggccagaa gcgcgagggc
     1561 cttcgtcccc ttggggtccg tcggcctgcc gggcaggtgg acctcaccgg cggatatcat
     1621 cgatgtcacg ccgccatgga gactgctttc gatatacccg agctggctct gacgaggagt
     1681 gaaatcgccg aagacgaagt gcacgtggga atcgatgaat cccgggcaga ccgtcatgcc
     1741 attggcatcg atggccttgt ccgccttagg ctctgtgccc tcgatggcct tgatcttccc
     1801 gtcctcgatg aggatggaga caggaccgtt cacaacaggt ctttcgatgt cgcctgtgaa
     1861 gatggttccc acattcctta taacgagcgt gctcatacgc ctcctccttt cactcggttc
     1921 atcccttgtt ctctttatcc ttctccaggg ccagcttcag cctctctatg tcgcatggta
     1981 gctggcgcat ccggactccc gtcgcgtcct tgatcgcgtt cacgatggcc gcggcctggg
     2041 gaatgagggc cggttcgccg acccctttgg ccccgaaagg tcccgtgggc tcgcggtctt
     2101 cgacgatcat cgcttcgacc tcgggcatgt ccatggacgt cgggagatag taattgtcga
     2161 aggacccgct cttcccgggt atgaactcct ccatgagcgc gaggccgatc cccatggcca
     2221 cacccccata gatctgtccc ctcacgctgc tcacattgat ggcccttccc acgtcgtggg
     2281 ccgcgtgcac cttgatgacc ttgcagacgc ccgtcacctc gtcgacgtcg acctgcgtca
     2341 tgtgtgtcgc gaaggcgtac gtggcgtagg gggcg
//
LOCUS       DAOQTR010000370         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_55441_length_2366_cov_3.127218, whole genome shotgun sequence.
ACCESSION   DAOQTR010000370 DAOQTR010000000
VERSION     DAOQTR010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55441_length_2366_cov_3.127218"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PKN85_08715"
     CDS             complement(<1..1085)
                     /locus_tag="PKN85_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT44532.1"
                     /translation="MWLADTSIRRPVTATMFLMALVVLGIVSYPSIGVDLYPKVDFPI
                     VNITTRLIGASPEIMDIDVTDKIEESINTINGVKTISSQSVEGTSVVTVEFELEKDID
                     LAVQDVREKISIIRAKLPTDIDEPIIQKVDPDATPVLWLSLSGSRSIGELSTYADEVL
                     QEKLQKVSGVGAVNVTGLRLRQVRIWLNLDKLKAYQITSSDVSQALARENVEIPGGRI
                     ESLSKEYSIKVKGEFPRVSDFNDLIVAYSRGAPVRIRDIGRAEDGLEERRSIARLNGE
                     TAVGLGIQKQSGTNTVEVVDLVKKELAKVQKTLPPGMNLSISFDQSRFIKTSINEVQH
                     HLIIGGLLAVLAVFLFLKNVRTTLISAL"
     gene            complement(1072..2181)
                     /locus_tag="PKN85_08720"
     CDS             complement(1072..2181)
                     /locus_tag="PKN85_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT44533.1"
                     /translation="MVRRLSSLFTHNPRFRAASAAWLLLPALLVAACGKQETRVADEK
                     TINVQVMTVETKSLRPFIETIGSLKAFEQVVVSSEIEGILRTTNVEEGAFVSKGSLLA
                     TVEDRDYASELRRVEFALKQAEATLVNAKAEYGRKETLYREELVTKQQFDDVATRLAV
                     ADAEVERSKAALAIAKERYAKTRILAPMAGYISEKKISQGEYVRNGTPLYTLVQIDPL
                     KLLFTVSEKDIGRLKVNQDVQFKVDAYPDREFKGRVTILYPTIDERTRSLTVEARAAN
                     PEKLLKPGLFAKVMLYTTEARDTVVIPVTSMLYEGKNVKVFVVEGDRAKERIVKVGNK
                     YDDTMEVVEGLKGGETLVVVGQQNLAEGVKVHVAR"
BASE COUNT          386 a          742 c          731 g          507 t
ORIGIN      
        1 agggcgctga tgagggtcgt ccggacgttc ttcaggaaga ggaacacggc caggacggcc
       61 agcagccccc cgatgatcag gtggtgctgc acctcgttga tggaggtctt gatgaaacgg
      121 gactggtcga aggagatgct gaggttcatc cccggcggca gggttttctg gaccttcgcc
      181 agttccttct tgacgaggtc gaccacttcg acggtattcg tccccgattg cttctggatg
      241 ccgagtccga cggccgtttc cccgttgaga cgggcgatgg accgcctctc ctcgaggccg
      301 tcctctgcgc gcccgatgtc ccggatgcgc acgggggccc ccctggaata ggccacgatg
      361 agatcgttga aatcggaaac gcgcgggaat tcccccttga ccttgatgga atactccttg
      421 gagaggctct cgatccgtcc cccggggatc tccacgttct cccgcgccag ggcctgcgag
      481 acatcgctgg acgtgatctg gtaggccttg agcttgtcca ggttcagcca gatgcgcacc
      541 tgccggagcc tcaggcccgt gacgttgacg gcccccaccc ccgaaacctt ctgcagcttc
      601 tcctggagaa cctcatcggc ataggtggac agctcgccga tcgagcggct gcccgacagg
      661 ctcagccaca ggaccggggt ggcatcgggg tccaccttct ggatgatggg ctcgtcgatg
      721 tcggtgggga gcttcgcgcg gatgatggag attttctccc gcacgtcctg cacggcgagg
      781 tcgatgtctt tctccagctc gaactcgacg gtgacgacgg aggttccctc gacgctctgg
      841 gagctgatcg tcttgacgcc gttgatcgtg ttgatcgact cctcgatctt gtccgtcacg
      901 tcgatgtcca tgatctcggg gcttgccccg atcaggcgcg tcgtgatgtt gacgatgggg
      961 aaatcgacct tggggtagag atcgacgccg atcgacgggt agctcacgat gcccaggacc
     1021 accagggcca tgaggaacat cgtcgccgta acgggcctcc ggatcgaggt gtcagcgagc
     1081 cacatggacc ttcaccccct ccgcgaggtt ctgctggccc acgacgacga gggtctcgcc
     1141 ccctttcaat ccctcgacga cctccatcgt gtcgtcgtac ttgttcccca ccttgacgat
     1201 ccgctccttc gcgcggtccc cctcgacgac gaacaccttg acgttcttgc cctcgtaaag
     1261 catggacgtc acggggatga cgacggtgtc cctcgcctcc gtcgtgtaga gcatgacttt
     1321 tgcgaacagt cccggcttga gcagcttctc ggggttggcg gccctcgcct cgaccgtgag
     1381 gcttcgcgtg cgctcgtcga tcgtcgggta gaggatcgtg acccgtccct tgaactcgcg
     1441 gtcggggtag gcgtccacct tgaactggac gtcctggttg accttgagcc ggccgatgtc
     1501 cttctccgac acggtgaaga gaagcttcag cgggtcgatc tggaccagcg tgtacagggg
     1561 cgtgccgttg cggacgtact ctccctggga gattttcttt tcactgatgt agcctgccat
     1621 gggggccagg atgcgcgtct tggcgtatcg ttccttcgcg atggccaggg ccgccttgga
     1681 gcgctcgacc tccgcgtcgg caacggccag gcgcgtcgcc acatcgtcga actgctgctt
     1741 cgtgaccagc tcctccctgt acagggtttc cttgcggccg tactccgctt ttgcgttgac
     1801 gagcgtcgcc tcggcctgct tcagggcgaa ctcgaccctc cggagctcgc tggcatagtc
     1861 ccggtcctcc accgttgcca gaagcgaacc cttcgacacg aaggcgccct cctcgacgtt
     1921 cgtggttcgc aggatccctt cgatctcgct cgacacgacg acctgctcga aggccttcag
     1981 cgaaccgatc gtctcgatga agggcctcag ggattttgtc tccaccgtca tgacctggac
     2041 gttgatcgtt ttttcatcgg ccaccctggt ctcctgtttc ccgcatgcgg caacgagaag
     2101 ggcaggaaga agcagccacg cggccgacgc cgcccggaat cgagggttgt gcgtgaagag
     2161 ggaagagagt cgcctgacca ttcggttcga ttcctccggt ttctctatgg tttcacgggt
     2221 tcagccggca cgccggacac gtaacccatc aggggggtcc ccgtgacgcg ctgcaatcgc
     2281 aagataaaat actggtagtt gtaggtcgcg tcggacatga gcttctgggc cgagatgagc
     2341 aggttgttgg cgtcgatgac gtcgag
//
LOCUS       DAOQTR010000371         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_55517_length_2364_cov_3.730619, whole genome shotgun sequence.
ACCESSION   DAOQTR010000371 DAOQTR010000000
VERSION     DAOQTR010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55517_length_2364_cov_3.730619"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKN85_08725"
     CDS             <1..509
                     /locus_tag="PKN85_08725"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT44534.1"
                     /translation="GYVTTAWWAVKFPLVEGFRHPVALYDAFKNLIIMFILLAVGRGG
                     TQGQGRLLGHFMLWYGVFDLVIDIFQAPGPLFRDFGIGRFSGALVALIGLLLIARSAR
                     RNSRKTTELSTLRFAPASLIAAIPSRLTPGIWFRVMLFMAILLFCLTIPGGWSREWLH
                     HLAWQLGI"
     gene            769..>2364
                     /gene="carB"
                     /locus_tag="PKN85_08730"
     CDS             769..>2364
                     /gene="carB"
                     /locus_tag="PKN85_08730"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNT44535.1"
                     /translation="MKKPPKIKGIDKVLIIGSGPIIIGQACEFDYSGTQACKALRAAG
                     YKIVLVNSNPATIMTDPGMADHTYVEPLTIDVIEKIIEKEKPQGLLPNLGGQTGLNLA
                     AELSRRGILEKHGVRIIGVQADAIERGEDRDEFKKTMKRLGIPMPESALAYSVEEALE
                     IADRLGYPVVIRPAYTMGGTGGGIAFNKEELRTIASRGISASIIGQILIEEAVVGWEE
                     LELEVVRDADNRMITVCFIENIDPMGVHTGDSFCCAPMLTISEEVQKKLQDYSYRIVE
                     AIQVIGGTNIQFAHNPEDGRIVVIEINPRTSRSSALASKATGFPIAFISAKLAGGYTL
                     KDIPYYRGKGLDEYVPTGEYVVIKFARWAFEKFSEAEDRLGTQMKAVGEAMSIGKTYK
                     EAFQKAIRSLETKRYGLGFAKNFNQLPLDDLMRLLNEPSSERQFIMYEALRKGAGIDE
                     LHEKTKIKRWFIEQMKEILDLEEEILRHKGGTLPDALLAKAKEDGFSDRYLSFLLGVP
                     ETDIRKKRQTLGKNETWCRVPVSG"
BASE COUNT          533 a          738 c          660 g          433 t
ORIGIN      
        1 atggttacgt gaccacggca tggtgggcgg tgaaatttcc cctcgtcgag ggcttccggc
       61 atcccgttgc cctctacgac gcgttcaaaa acctcatcat catgttcatc ctccttgccg
      121 tgggacgagg gggcacccag gggcagggca ggcttctcgg ccacttcatg ttgtggtatg
      181 gggtgttcga tctggtcata gacatcttcc aggcgcccgg tcctcttttc agggacttcg
      241 gcatcggccg tttttcaggc gctctggtgg ccctcatcgg cctcttgctc atagcgagat
      301 cggcgcgaag gaactccaga aagaccacgg agctcagcac cctgcgtttc gcgccggcgt
      361 cgctcatcgc cgcaatacct tcccgcctca ccccggggat ctggttcagg gtgatgctct
      421 tcatggcgat actccttttc tgcctcacca tccccggcgg atggagccgg gagtggctcc
      481 accatctcgc ctggcagttg ggaatatgaa acagagaaaa acggcggccc acagggtgcc
      541 cgctcacccg acgaggcggc gggccctccc ctctccagag tatcccttcc gggccgtcga
      601 tcaccgcccg gttggtcata gtttctcctg taactacaag tagttggcga acaggtcact
      661 atttttatcc cgccaccggt taccaaaacc cgaaatcctt tgaaaacgct tgactgactg
      721 ccactcatct gttacgatgc agagcaaatc cgaaaggaat ccccggcaat gaaaaaaccc
      781 cccaaaatca aaggcatcga caaggtcctc atcatcggct ccggtcccat catcatcggg
      841 caagcgtgcg agttcgacta ctccggcacc caggcctgca aggccctgag ggctgccgga
      901 tacaagatag tcctcgtgaa ttccaacccc gcgacgatca tgaccgatcc cgggatggcg
      961 gatcacacat acgtggaacc cctcaccatc gacgtcatcg aaaagatcat agaaaaggag
     1021 aaaccccagg ggctcctccc caatctggga ggtcagacgg gtctcaacct tgccgcagag
     1081 ctgtcccgcc gggggatact tgagaagcac ggggtccgca tcataggagt ccaggccgac
     1141 gcgatagagc gcggcgagga ccgtgacgag ttcaagaaga ccatgaagag actgggcatt
     1201 cccatgcccg agagcgctct cgcctactcc gtggaagaag ccctcgagat cgcggacagg
     1261 ttgggatatc ccgtcgtcat ccgccccgcg tacaccatgg gcggcacggg aggcggcata
     1321 gccttcaaca aggaggagct gcggaccata gcgagccggg gcatctccgc cagcatcatc
     1381 gggcagatcc tcatcgagga agccgtggtg ggctgggaag agctggaact cgaggtcgtc
     1441 cgtgacgcgg acaacagaat gatcaccgtc tgcttcatcg agaacatcga tcccatgggc
     1501 gtccacaccg gcgactcatt ctgctgcgcg ccgatgctca ccatttccga ggaggtgcag
     1561 aagaagcttc aggattactc ctaccggatc gtggaggcca tccaggtcat cggcggcacc
     1621 aatatacaat tcgcccacaa ccccgaagac ggccgcattg tcgtcatcga gatcaacccc
     1681 aggacatccc gctcgtcagc tctcgcctcg aaggcaacgg gcttccccat cgccttcatc
     1741 tcggccaagc tggcaggcgg ctacaccctg aaggacatcc cctattaccg cgggaagggg
     1801 ctcgacgaat acgtccctac gggagagtac gtggtcatca agttcgcccg ctgggccttc
     1861 gagaagttca gcgaggcaga ggacaggctg ggcacccaga tgaaggccgt cggcgaggct
     1921 atgagcatcg gcaagaccta caaggaagcg ttccagaagg cgatccgttc cctggagaca
     1981 aaacgctacg ggcttggttt cgccaagaat ttcaatcaac tgcctctcga cgacctgatg
     2041 cgcctgctca acgaaccctc ctctgaaagg cagttcatca tgtacgaagc gctgcgcaag
     2101 ggtgccggca tcgatgaact ccatgagaag acgaagatca aacggtggtt catcgaacag
     2161 atgaaggaga tactcgatct cgaggaagag atccttcgcc acaagggagg cacgctcccc
     2221 gatgccctcc tcgccaaggc aaaagaggac ggattctccg acaggtatct ctccttcctc
     2281 ctcggcgtcc ctgaaacgga catcaggaag aaacggcaga ccctcggcaa gaatgagaca
     2341 tggtgccgcg tacccgtcag cggc
//
LOCUS       DAOQTR010000372         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_55812_length_2357_cov_2.228931, whole genome shotgun sequence.
ACCESSION   DAOQTR010000372 DAOQTR010000000
VERSION     DAOQTR010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55812_length_2357_cov_2.228931"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PKN85_08735"
     CDS             <1..877
                     /locus_tag="PKN85_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNT44536.1"
                     /translation="DGPMPQTREHVLLARQVNVPALVVFMNKTDQVDDPELLDLVEME
                     VRELLSKYEFPGDDIPVIRGSALKVMEEGNGTREDPISKCIWELMDACDSYIPAPVRE
                     VDKPFLMPIEDVFTITGRGTVVTGRVERGVIKGGEEVAIVGMKTDVTKTVATSLEMFR
                     KILDSAMAGDNVGVLLRGVDKDGVERGQVLAKPGSITPHTKFKAEVYVLKKEEGGRHT
                     PFFAGYKPQFYFRTTDVTGGIKLPEGVEMVMPGDNATFEVDLIVPIAMDPGLRFAVRE
                     GGHTVGAGVVTEILE"
     gene            898..1203
                     /gene="rpsJ"
                     /locus_tag="PKN85_08740"
     CDS             898..1203
                     /gene="rpsJ"
                     /locus_tag="PKN85_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNT44537.1"
                     /translation="MAKKIRIRLKAFDHRVLDSSASQIAETADRSGAKVSGPIPLPTE
                     INKVTILKSPHKDKDAREQFETRTHKRLIDIINPTQKTMDALMQLNLPSGVDIQIKL"
     gene            1237..1863
                     /gene="rplC"
                     /locus_tag="PKN85_08745"
     CDS             1237..1863
                     /gene="rplC"
                     /locus_tag="PKN85_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNT44538.1"
                     /translation="MSIGILGKKLGMAQIYNDQGQAVAVTVIQAGPCPVVDMKTPDKD
                     GYSALVLGFGKVKPKNVNKSRQGLFGKAEVEPQNTLREFRVDDLDGYSVGQEINVTLF
                     SEGETVNVSGISKGKGFAGVMKKYHFGGSNSSHGASVVHRRGGSSGASSYPGRVFKGK
                     RMPGRMGSEKVTVKNLTVVAVDTDNNLLLVKGAVPGAKNSLVTLFKKG"
     gene            1987..2357
                     /gene="rplD"
                     /locus_tag="PKN85_08750"
                     /pseudo
     CDS             1987..2357
                     /gene="rplD"
                     /locus_tag="PKN85_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
BASE COUNT          554 a          649 c          732 g          422 t
ORIGIN      
        1 cgacggtccc atgccccaga cccgtgagca cgtgcttctt gcccgccagg tcaacgtgcc
       61 ggccctcgtg gtcttcatga acaagacgga ccaggtggat gatcccgagc ttcttgacct
      121 tgtggaaatg gaagttcgtg agctgctgag caagtatgaa ttccccggag acgacatccc
      181 cgtcatccgc ggctcggcgc tgaaggtcat ggaagaaggg aacggcaccc gtgaggatcc
      241 catcagcaag tgcatctggg agctcatgga tgcgtgcgac agctacatcc ccgcgcctgt
      301 gcgcgaagtt gacaagcctt tcctcatgcc catcgaggac gtgttcacca tcaccggccg
      361 cggcaccgtg gtcacgggca gggtggagcg tggagtcatc aagggcggcg aagaagtggc
      421 catcgtcgga atgaagacgg acgtcacgaa gactgtagcc acgtcccttg aaatgttccg
      481 taagattctg gacagtgcca tggcgggaga caatgtggga gtgctgcttc ggggagtgga
      541 caaggacgga gttgagcgcg gccaggtgtt ggcgaagccg ggttcgatca cgccgcacac
      601 gaagttcaag gcagaagtgt acgtactgaa gaaggaagag ggcggacgac acacgccgtt
      661 ctttgcgggg tacaagcccc agttctactt ccggaccacg gacgtgacgg gtggcatcaa
      721 gcttcccgag ggagtggaaa tggtcatgcc gggagacaac gcgacgtttg aagtggacct
      781 gatcgtgccc atcgccatgg acccgggtct tcggtttgcg gtgcgcgagg gcggccatac
      841 ggtcggcgcc ggcgtcgtca ccgagattct tgaatagccc cagaggggga atgaacagtg
      901 gcaaagaaaa ttcgaatccg gctgaaggct ttcgaccaca gggttcttga cagctcagcc
      961 tcccagatag ccgaaacggc tgatcggagc ggcgccaagg tttccgggcc gatacccctt
     1021 ccgaccgaga tcaacaaggt tacgatcctg aagtcccctc acaaggacaa ggacgcgcgc
     1081 gaacagtttg aaaccaggac acataagcgc ctcatcgaca tcatcaaccc gacgcagaag
     1141 accatggacg cgcttatgca gctgaacctt ccttctggag tggacatcca gatcaaattg
     1201 taggggaagc tctgagctga aaggagttgg agtgaaatga gcatcggaat tctgggtaag
     1261 aaactcggaa tggcccagat atacaacgat cagggccagg cggtggcggt cacggtgatc
     1321 caggccggcc cgtgccctgt agtcgatatg aaaaccccgg acaaggacgg gtacagtgcc
     1381 cttgttctcg ggttcggaaa ggtcaaaccg aagaatgtca acaagtcccg ccagggactt
     1441 ttcgggaagg ccgaagtgga acctcagaac acccttcggg agttccgggt tgacgacctc
     1501 gacggatatt ccgtcggcca ggagatcaac gtgactcttt tctccgaggg agaaacggtg
     1561 aacgtgtcag gaatcagcaa gggtaagggg tttgccggag tcatgaagaa gtaccacttc
     1621 ggcggttcga attccagcca tggcgcttcc gtggttcacc ggcggggcgg ctccagcggg
     1681 gccagcagct atcccggccg cgttttcaag ggcaagagaa tgcccggaag aatgggcagt
     1741 gagaaagtaa cggtcaagaa cctcactgtc gtagcagtgg acaccgacaa caaccttctt
     1801 cttgtcaagg gtgcggtacc cggcgcgaaa aacagcctgg tcaccctttt caagaaaggc
     1861 tagtcggcag agaaggaggc cggaattaca atgcctaccc tgaaactcgt atcattccag
     1921 ggggaacagg tcggggagat gcagcttgcc gatgaagtct tcggcgcccc tgttcatgtt
     1981 cccgccatgc accaggtggt ggtcgcccag ctcgccaacc ggagacgtgg aacacagtcc
     2041 gcgaagacca ggggcgaagt gagcggcggc ggcaagaaac cctggagaca gaagaagacc
     2101 ggacgggccc gtcacggaag cacccgttct cccatctgga ccgacggcgg tgtgacccat
     2161 gctccgaagc cccgtgatta cagccagaag gtgaacaaaa aggtacgccg gctcgcgatc
     2221 cgcagcgctc tctcgctgaa agtccgggac gagctcatga ctgttgtcca gtcctttgat
     2281 ctttccaagc cctccacgaa ggaaatgatc gccttctttt cggccgttaa cgccaggaag
     2341 ccgctgatcg tccttcc
//
LOCUS       DAOQTR010000373         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_56305_length_2343_cov_4.154720, whole genome shotgun sequence.
ACCESSION   DAOQTR010000373 DAOQTR010000000
VERSION     DAOQTR010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56305_length_2343_cov_4.154720"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKN85_08755"
     CDS             complement(<1..723)
                     /locus_tag="PKN85_08755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT44539.1"
                     /translation="MKDYDVLVIGSGSGLSIVYKALGAGLKTALVAREYLGGTCLNVG
                     CVPSKTLLYTADRVMEIGEAKRFGVGAEVSSIDFPSIMTRMRKAVQDGTASIRSGLKE
                     EADLDFYEEEGQFVDDYTLMAGKEEIRAKTIFVASGARPAMPPVKGLAGIGCLTNESV
                     LQLEKLPESIIIIGGSYVGVEYAHFFAAMGSAVSIVEYADRLVPFEEPEISETLESSF
                     AKRMGVYTAHEALAVEKGDGGFT"
     gene            complement(888..1814)
                     /locus_tag="PKN85_08760"
     CDS             complement(888..1814)
                     /locus_tag="PKN85_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxygenase reductase family protein"
                     /protein_id="HNT44540.1"
                     /translation="MAPKGKSDSIHVAVNMSRVVNSEESFRQMHQVGPRVCITTASHP
                     GFLGFQANVQTGILPLAGRFGGGAVDMRQTLNPIRNYQYTMWKTWQDHDDFHMKQFDR
                     IFELCGHCLSMVVEGPWEPVYSIVKAKMPPLRSMGQIADLAQDIQQQKDFVRFATPMR
                     CVALAEHTVKPGREKDFEAGAIETMEAISDSTGFLGYMIMKQIGVCAIGSFMLDPRSM
                     GEALQTLGAVPPRNPKPAFETPDAAPNPPEYLIHSEWEAPEMAQLGFAKVLVNHEVRN
                     IHDKGVMAHLVKGPYIMFFQPMMEEPGWRKYL"
     gene            2143..>2343
                     /locus_tag="PKN85_08765"
     CDS             2143..>2343
                     /locus_tag="PKN85_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44541.1"
                     /translation="MGELQEKAIVKDAAGETHQGVRSRQRQPVYWRNFKKPGDEVLKK
                     RLTPLQYEVTQKEGTEPAFRNEY"
BASE COUNT          481 a          695 c          646 g          521 t
ORIGIN      
        1 cgtgaaacct ccatcacctt tctcaacggc aagggcctcg tgggccgtgt agacccccat
       61 ccttttcgcg aaggagctct ccaatgtctc cgatatctcc ggctcttcga aggggacaag
      121 ccggtcggca tattcgacta tggagaccgc gctccccatg gcggcgaaga aatgggcgta
      181 ttcaacgcct acatagctgc ctcctatgat gatgatgctt tccggcagct tttccagctg
      241 aaggacggac tcattggtca ggcagccgat accggccagg ccttttacag gtggcatggc
      301 cggccgcgcg ccggaggcga cgaatattgt ctttgccctg atctcttcct ttcccgccat
      361 tagtgtatag tcgtcgacga actgcccctc ttcttcgtag aaatcaaggt cggcctcttc
      421 cttaaggccg cttctgatgg acgcagtgcc atcctgtaca gccttcctca ttcttgtcat
      481 gatcgaagga aaatctatcg agctgacctc agcgcctacc ccgaatctct tcgcctcccc
      541 gatctccatc accctgtcgg cggtgtacag gagcgtcttg gaagggacgc accccacgtt
      601 gaggcaggta cccccgaggt attcccttgc gacaagggcg gttttcaggc ccgccccaag
      661 ggccttgtag acgatgctca aaccggaccc cgagccgatc acaaggacgt catagtcttt
      721 catgaaacac ctcctcgtct tcgcacgata gcctccatgc cgccttgagc cgttcgcgga
      781 aaacggtgcg cacatctcat catggtgaag atcacggtca caagcagata cgcgaaggac
      841 accccgggtt cactcttttt tccgggtgac gccttccgtc gctgccgcta gaggtacttc
      901 ctccagccgg gttcctccat catgggttgg aagaacatga tgtacggccc cttgaccagg
      961 tgggccatca cgcccttatc gtggatattg cggacctcgt ggttgaccag aaccttggcg
     1021 aagccgagtt gggccatctc gggtgcttcc cactcgctgt gaatgaggta ctcaggcgga
     1081 ttgggcgcgg cgtcgggcgt ctcgaacgcc ggtttcgggt ttctgggagg caccgcccca
     1141 agggtctgaa gggcctcacc catggatcgc gggtcaagca tgaaactgcc gatagcgcac
     1201 acgccgatct gcttcatgat catgtacccg aggaaacccg tcgaatcgga gatggcctcc
     1261 atcgtctcta tcgcgccggc ctcgaaatcc ttttccctgc ccggcttgac ggtatgctcg
     1321 gcaagggcca cacaacgcat gggtgtggca aatcttacaa agtctttctg ctgctggatg
     1381 tcctgtgcaa ggtcggctat ctggcccatg gaccgaaggg ggggcatttt ggccttaacg
     1441 atgctgtaga ccggctccca cggtccctcg acaaccatgg aaagacagtg gccgcaaagc
     1501 tcgaagatcc tgtcgaattg cttcatgtga aaatcgtcat gatcctgcca ggtcttccac
     1561 atggtatatt ggtaatttct tatggggttc agggtttgcc gcatatcgac ggcaccaccc
     1621 ccgaaacggc cggccagggg gagtatgccc gtctgaacgt tcgcctgaaa tccgaggaac
     1681 ccgggatggg acgcggtagt gatacacacc ctgggaccca cctgatgcat ctggcggaag
     1741 ctctcctcgg agttcaccac cctggacata ttgaccgcca catggatcga atccgacttg
     1801 cccttcggcg ccatggtctt tacctcctcc ttccagtgat cactcaatat gcatgaaaga
     1861 tcctgccggc atccatgtcg tccggctcat aatgtgagtc tatttggcca cccctgttct
     1921 gtcaagttcg ccggggcctc catttggtcc gtcgcggcag acagggcggg caggggacat
     1981 gttcgggttt gacgggaaat gactcgatcc gctctcgacg cggcttgcac ggaaggaata
     2041 aatgattata ttcgtcgatc gtaaggagga catatgaaac tttcatcctc tatcgcgttt
     2101 gcgcttctta tgctgatccc ctatggttgt cagaataccg gtatgggcga actgcaggag
     2161 aaggccatcg tgaaggacgc ggcaggtgaa acgcatcagg gcgtgcggtc ccgccagcgg
     2221 caacccgttt actggaggaa tttcaagaaa cccggagatg aggtactgaa gaaacggctc
     2281 acccccctcc agtatgaggt gacgcagaaa gaggggacgg aaccggcctt ccggaacgag
     2341 tac
//
LOCUS       DAOQTR010000374         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_56309_length_2343_cov_3.955420, whole genome shotgun sequence.
ACCESSION   DAOQTR010000374 DAOQTR010000000
VERSION     DAOQTR010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56309_length_2343_cov_3.955420"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PKN85_08770"
     CDS             complement(<1..668)
                     /locus_tag="PKN85_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNT44542.1"
                     /translation="MNRTDTETRGDKGFYNRSLERALQIMCAFTSERNAYTLTQLTEA
                     VRLPKATTIRLCSTLIKYGFMKFDHASMQYSLGIKLFELGSIVYSSLSLRKTASPFLH
                     QLQVRSGKTCFLGILENDELVYLDKRESPGNPIRFASQIGRIRPPYFGMLGQVLMATL
                     PEEEVDKILQKTPLASHTKKTTTDPYEFREKLARIRKQGYIIEDGEAIEGISGIAAPI
                     YNFTG"
     gene            1023..1508
                     /locus_tag="PKN85_08775"
     CDS             1023..1508
                     /locus_tag="PKN85_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNT44543.1"
                     /translation="MQSFLKGVHRVILFMQGIAGVVLTFVIVLTTADVLLRIFGHPVP
                     GAVEITAMCGGIVVGFTVPMTSWLKGHIAVDFLLNNWTAKVRNTVETVTRCVGIAMFI
                     FIGVNMVRIGRGFQVANEVTGTLELPMYPVVYGIAGGLFALSVVLLCDILKIYGGEGN
                     E"
     gene            1501..>2343
                     /locus_tag="PKN85_08780"
     CDS             1501..>2343
                     /locus_tag="PKN85_08780"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT44544.1"
                     /translation="MNEITVGIIALVVLLALFFTGLELPFCMILVGFSGFAYLVDFTA
                     ASHMVASDVYEVFASYAYTVFPLFIFMGQIAFVSGIAGKLYDSAYKFFGHIPGGLAIA
                     TVGGATAFKSLCGSAVATAATFSNLAIPQMNRYGYSRTLGTGLVAIVGTLGCLMPPTI
                     FLIIYGLITEQSIGALFLAGIFPGLGIAVLWIVTIFIWAKFNPGLAPAGGKSTWKERF
                     ASLPEVAVVLGVFLLMIVGLLKGFFTPTEAGGIGVAAVLVMVIVGRQLSFKSYVTAMG
                     EGLRT"
BASE COUNT          449 a          617 c          694 g          583 t
ORIGIN      
        1 cccgtgaaat tgtatatggg ggcagcgata cccgagatgc cttcgatggc ctctccgtct
       61 tcgatgatgt agccctgctt gcgtatcctt gccagttttt cccggaactc gtaggggtca
      121 gtggtggtct ttttcgtatg ggaagccaga ggggtcttct gcaggatctt gtccacctcc
      181 tcctcgggaa gcgtggccat gagcacctgg ccgagcatgc cgaaataggg cggacggatc
      241 ctgcctatct gggaggcgaa acggatgggg ttcccggggc tttccctctt gtcaaggtag
      301 acgagttcgt cattctccag aatacccagg aagcaggtct tccccgatct tacctggagc
      361 tggtgcagga aaggagacgc ggtctttctg agggagagcg atgagtagac tatgctcccc
      421 agctcaaaga gcttgatgcc cagcgaatac tgcatggacg catggtcgaa tttcatgaaa
      481 ccgtatttga tgagggtgga gcagagcctg atagtcgtgg ccttgggcag ccgaacggct
      541 tctgtcaact gcgtgagggt gtaggcgttc cgctcgctgg tgaaggcgca catgatctgg
      601 agggccctct ccagggaccg gttgtagaat cccttgtcgc ctcgtgtttc agtgtcggtc
      661 ctgttcataa tagttcacct catggatacc tatttcacta tatgaaatac tattatatga
      721 aatatgtcaa gctaacctgt caaagaattc ttctgtagtc tcgcatggcg catatattgt
      781 ataaaggaaa actttctcac tgtcgagaat aaatgtcttg acaagtttca ttttccgctg
      841 ataaaatcac agaatgaaat atgtaaccat tatgtggaac aattaccata tccaatcctg
      901 ccggagaatc aggttgttca atgcagatag aggggcgagc ccggtcctcc tgtcggggcg
      961 aaggacggag ttgggcgggt agatacgaca gaaactgctc agatcttgcg cgggaggtat
     1021 ttatgcagtc attcttgaag ggtgttcaca gggtcattct gttcatgcag ggcattgcgg
     1081 gtgtcgtcct gaccttcgtg atagtcttga ccacggcgga tgtgctcctg aggatattcg
     1141 ggcaccccgt ccccggtgcc gtcgagatca ctgcgatgtg cgggggaata gtggtgggct
     1201 tcaccgtacc catgacctcc tggctgaaag gtcacattgc cgtcgacttc cttctcaaca
     1261 actggacggc gaaggtgagg aacacggtcg aaacggtcac gaggtgtgtc gggatagcga
     1321 tgttcatctt tatcggggtc aacatggtaa ggatcgggcg gggcttccag gtagccaacg
     1381 aggtgacggg cacgctcgag ctgccgatgt accccgtggt ctacggaata gccgggggtc
     1441 tgtttgccct gtcggtcgtt ctcttgtgcg acatcctgaa gatctacgga ggagaaggca
     1501 atgaatgaga tcacggttgg aatcatagct ctcgtggtgc tgctggcact cttttttacc
     1561 gggctggaac tgcccttctg catgatcctt gtcggcttct ccggatttgc ctacctcgtg
     1621 gattttaccg ccgcgtccca catggtcgcg agcgatgtgt atgaggtctt cgcgtcctac
     1681 gcgtacaccg tctttcctct cttcatcttc atgggccaga tcgcttttgt gtcgggcata
     1741 gcagggaagc tttacgacag cgcctacaag ttcttcgggc acatcccggg aggactggcg
     1801 atcgcgaccg tcggtggggc aacggccttc aaatcgctct gcggttccgc cgtcgcgacg
     1861 gcggccacat tctcgaacct tgccatcccc cagatgaacc gctacgggta ttcgaggacg
     1921 ctcggcaccg gcctcgtggc gatcgtcggg acgctgggct gcctgatgcc gcccacgatc
     1981 ttcctcatta tatatggctt gatcacggag cagtccattg gagccctctt cctggcgggc
     2041 atcttcccgg gccttgggat tgccgttttg tggatcgtga cgatcttcat ctgggcgaag
     2101 ttcaaccccg gcctggcacc cgccggcggt aagtccacgt ggaaggagag gttcgcatcc
     2161 ctgcctgagg tcgctgtggt cctcggcgtg ttcctcctca tgatcgtcgg gcttctcaag
     2221 ggcttcttca ctcccaccga ggcaggcggg atcggcgtcg ccgccgtcct tgtcatggtc
     2281 atcgtgggcc ggcagctttc gttcaagagc tatgtgacgg ccatgggaga gggtctgcgc
     2341 acc
//
LOCUS       DAOQTR010000375         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_56492_length_2338_cov_3.600088, whole genome shotgun sequence.
ACCESSION   DAOQTR010000375 DAOQTR010000000
VERSION     DAOQTR010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56492_length_2338_cov_3.600088"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1254
                     /locus_tag="PKN85_08785"
     CDS             202..1254
                     /locus_tag="PKN85_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT44545.1"
                     /translation="MKRDIPDIYEFAASKRVSLSRIMDFTSTVNPIGPPAKARNAIRK
                     NLGNLGCRPDPEARYLVRAIARAEGVSENNVLVGESLETLVAALLHAFGDRTLLIEAP
                     YPAYLRDLLEGPEEPRFFSLDGGDGFRFDPFRFAAEMRHCDAAILASPSFLSSETLSR
                     DGARGIISEAGEHHTLLIIDETLKGYSGTPSLAADILQREGCVVMGSLGEYYGLAGLS
                     VSYVIGTAAVIEDMRRRKAVTPPNTLAAAAATASLRDPTFSARTRTFMERERSFIAEG
                     LRGIPGISFSSSACGSFIVTLVDDPSKPLETFEHYRIVVDDLGDAGTLFFPVKDHKWN
                     ARYLKTLKNIMGTPKQ"
     gene            1251..>2338
                     /locus_tag="PKN85_08790"
     CDS             1251..>2338
                     /locus_tag="PKN85_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNT44546.1"
                     /translation="MKRTTTSTLFLLISLVIFLFTLHLAPACAASLRILHVNDFHGFA
                     ESHKRLGSDDVRGGVACLAYLAETLRKEKPSLLLAAGDMIQGSTWSNLFEGRSVIEVM
                     NVMRFDAMVVGNHEFDFGKTVLTELTREADFPVLGANVTGLDALKPYIVKEVDGIRVG
                     IIGVVTEETPTATHPRNVEGLTFMPAAKTVERCIGELKGRADIIVVLSHLGYNADMAL
                     ASTVKGIDVIVGGHSHTKGVKPITVGSTVIVQAWEHGLVLGVLDLTVESGRIVAVEGR
                     LEDIVPEKMARDEKVAAIVEKWAGKVRESMGKVIAEAEVDLDGENVRVRETNLGNFIA
                     DILKKESGADAAIINGGGLRTSIKKGPVT"
BASE COUNT          480 a          715 c          680 g          463 t
ORIGIN      
        1 atggctcttc aactactgag ccttaagttg taaacgcttt tagtgacacg tgcaggcagg
       61 acaggcgaca tgcaacggca accaacggac cgtcccattt tcttctgttc cctcaccctt
      121 gaacttttga acccttgaac cgtttttggg ggttggctga ctttttccgg ggtttcgtga
      181 tataacattt atcacgtgtt catgaaaaga gatatccccg acatttacga atttgccgca
      241 tcgaagagag tctctctttc gcgcatcatg gacttcactt cgacggtaaa cccgatagga
      301 ccacccgcaa aggccaggaa tgccatcagg aagaacctcg ggaacctcgg ttgtcgtccc
      361 gaccccgagg cgagatacct ggtccgggcc atcgctcgcg cggaaggcgt atcggagaat
      421 aacgtcctcg tcggcgaaag tctcgagacg ctggtcgcgg ccctgctgca tgccttcggc
      481 gaccggacgc tcctcatcga agcaccctac cccgcatacc tgcgggacct gctggagggt
      541 ccggaggaac cccgcttctt ttccctcgac ggcggggacg gtttccgctt cgacccgttc
      601 cggtttgctg cggaaatgcg gcactgtgac gcggccatcc ttgcatcccc ctcttttctg
      661 tcctcggaga cactatcccg cgacggggct cgcgggatca tttcagaggc cggggaacac
      721 catacccttc tcatcatcga cgaaaccctg aaaggatact ccggcacacc ctcgctcgct
      781 gccgacatcc tgcagaggga gggatgcgtc gtcatggggt cccttggcga gtattacggc
      841 ctcgccggcc tgtccgtctc ctacgtcatc ggaacggccg ccgtgatcga ggacatgagg
      901 cggcgaaaag cggtgactcc ccccaacaca ctcgccgccg cggcggcgac ggcgtcactg
      961 cgcgacccga cgttctccgc gcggacgagg acgttcatgg aacgggagag atccttcatc
     1021 gccgagggtc ttcggggtat cccgggaatt tccttctcca gctctgcctg cggttctttc
     1081 atcgtgaccc tcgtcgacga cccgtcaaaa cccctcgaga ccttcgaaca ctacaggatc
     1141 gtcgtcgacg acctgggaga cgcagggacc ctgttctttc ccgtcaaaga ccacaagtgg
     1201 aacgcgcgtt acctgaaaac tctgaagaac attatgggaa caccgaaaca atgaaaagaa
     1261 caacgacctc gacactattc ctgcttatat ccctcgtcat ctttctcttc accctgcacc
     1321 tcgcgcctgc ctgcgccgct tccttgcgta tcctccacgt gaacgatttc catggcttcg
     1381 ccgagagcca caagcgcctg gggtccgacg acgttcgcgg gggcgtcgcc tgcctcgcct
     1441 atctcgcgga gaccctgagg aaggaaaagc cttccctcct ccttgccgcc ggtgacatga
     1501 tacagggcag cacctggtcg aacctcttcg agggaaggtc cgtcatagag gtgatgaacg
     1561 tcatgcgttt cgacgccatg gtcgtcggca accatgaatt cgatttcggc aagacggtgc
     1621 tcacggaatt gaccagagag gcggatttcc ccgtcctcgg ggccaacgtg accggcctcg
     1681 acgcgctcaa gccttacatc gtcaaggagg tggacggcat cagggtgggt atcatcggtg
     1741 tcgtcacaga ggagaccccc acggccacgc acccccggaa tgtcgagggg ttgaccttca
     1801 tgcccgcagc gaagaccgtc gaacgctgca tcggcgagtt gaagggcagg gccgacatca
     1861 tcgtggtcct ctcgcacctc gggtacaatg ccgacatggc cctcgcgtcg actgtcaagg
     1921 gcatcgatgt catcgtgggc ggacattccc acacgaaggg agtgaagccg atcacggtgg
     1981 gcagcacggt gatcgtccag gcctgggagc atggtttggt attgggggtc ctcgacctca
     2041 cggtggagtc gggcaggatc gtcgccgtgg aaggcaggct cgaggatatc gtgcccgaga
     2101 agatggcaag ggacgaaaag gtcgccgcga ttgtcgagaa gtgggccggg aaggttagag
     2161 aatccatggg gaaggtgatc gccgaggccg aggtcgatct cgatggagag aacgtgcgcg
     2221 tgagggagac caatctcggc aatttcatcg ccgacatcct caaaaaggag tccggcgccg
     2281 atgccgccat catcaacgga ggcgggctcc gtaccagtat aaagaagggt cccgtcac
//
LOCUS       DAOQTR010000376         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_57106_length_2322_cov_3.518747, whole genome shotgun sequence.
ACCESSION   DAOQTR010000376 DAOQTR010000000
VERSION     DAOQTR010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57106_length_2322_cov_3.518747"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..871)
                     /locus_tag="PKN85_08795"
     CDS             complement(158..871)
                     /locus_tag="PKN85_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNT44547.1"
                     /translation="MRTSLRRLGANGARGPMKLCDHRLVRNRPSSCYLIHLHNGSRAR
                     YREHRTLFRDDRTRGLDLPIHLHCDEEMSEICPFRQWSPKLKSEPARSFWIECEGCGK
                     RHREEQAHQTCVSWHNFLALYRSKETKAWRPEGTTDDYLSIGPESELYEPVRRWLWRR
                     IRRYVKRRDGGRCTECGIEVKGRRVPYEIHHIIPRSRGGTEHPANLRTLCSSCHQRYT
                     KELLRDNLERRRGGAIRER"
     gene            870..1004
                     /locus_tag="PKN85_08800"
     CDS             870..1004
                     /locus_tag="PKN85_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44548.1"
                     /translation="MVFYTFSLPFRKYIDHLQVEIAADPSLERPHIPVPHAFLNVRKI
                     "
     gene            1057..1818
                     /locus_tag="PKN85_08805"
     CDS             1057..1818
                     /locus_tag="PKN85_08805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT44549.1"
                     /translation="MTKESSTAKVSKGDIVHVEYNAYLAENGMLFDTTNADKAKEAEI
                     FNENLTYKPLPLLVGGGRVFPGLDEALAEAKVGELTTVEIPPEKAAGARDPKLVENIA
                     LREFFRQEIRPEIGMEVNIKNRVGTVVTVTNRMVRVDFNRRFAGSALRYEFTVLDKVE
                     GDEAKVMAICEMDYGTAEGFRATVADGKVVLYLPDVCKYDQKWLLTKYKIVADLREVF
                     GPIDVELVEEYLKKEDKPAEEKPAEVVEEKAPEEL"
     gene            1873..1947
                     /locus_tag="PKN85_08810"
     tRNA            1873..1947
                     /locus_tag="PKN85_08810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1908..1910,aa:Arg,seq:cct)
     gene            2020..2256
                     /locus_tag="PKN85_08815"
     CDS             2020..2256
                     /locus_tag="PKN85_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44550.1"
                     /translation="MWANEYYHVMDNHDKIIEMMMSTGLKTYPEHLNAEDAFEFIGSV
                     KEGLMRTHPRQGDGSALFPFKHLMMVAKKVQAIH"
BASE COUNT          478 a          717 c          649 g          478 t
ORIGIN      
        1 cacacctctc tcaccggtgt tccgaccatc gccataggcg ttccgacccg tcacattcac
       61 tctcatgtgg gcctgttcga cacggccgat ctggatgcct gtatgaaatt ggtcgtcgag
      121 ctggccaagg cgctcgaccg gaagacggtg gactcgctca ccgctctctg atcgcgccgc
      181 cgcgtcggcg ttcgaggttg tcccgcagga gctccttggt gtacctctga tgacagctcg
      241 agcaaagcgt tcgcaggttg gccggatgct ccgtccctcc ccgggacctg gggatgatgt
      301 gatgtatctc gtaagggacc cgccttccct tcacctctat cccgcattcg gtgcatcgtc
      361 cgccgtccct gcgtttgacg taccgccgga tccttctcca cagccatctg cggaccggtt
      421 cgtacagttc cgattccggc cctatggaca ggtagtcatc ggtggtgcct tccgggcgcc
      481 aggccttcgt ttctttcgag cggtataacg ccagaaaatt gtgccatgag acgcatgtct
      541 ggtgcgcctg ctcctcgcga tgccgtttgc cgcagccctc gcattctatc cagaagctcc
      601 tggccggctc gctcttcaat ttaggtgacc attgccggaa cgggcatatc tcggacatct
      661 cctcgtcgca gtgcaggtgg atgggcagat ctaggccgcg ggtccggtcg tccctgaaca
      721 gggtccgatg ctccctgtat cgagccctag agccgttgtg caggtgaatg agatagcagg
      781 aggacggacg gttcctgacc agacgatgat cgcagagctt catgggaccc cgggcgccat
      841 tggcgcctag acgccgaagc gatgtcctca tggtctttta caccttttcc cttcctttcc
      901 gaaagtacat agaccacctt caggtcgaaa tagcggccga cccctccctg gaacgccccc
      961 acatcccagt tccccacgcc ttcctgaacg tccgaaagat ataaaaagag ggaatcaatc
     1021 aaccagtccg cctaaatagc attactggtg aatttcatga ccaaggaatc ttcgaccgca
     1081 aaggtctcca agggtgacat tgtccacgtt gaatacaacg cctatctcgc cgagaacggc
     1141 atgctcttcg acacgaccaa cgcggacaag gccaaggagg ccgagatctt caacgagaac
     1201 ctgacctaca agccgctgcc cctgctggtc ggcggcggaa gggtcttccc cggtctggac
     1261 gaggccctgg ccgaggccaa ggtcggagag ctgaccaccg tggagatacc cccggagaag
     1321 gccgctggcg cccgggaccc caagctggtc gagaacatcg ccctgcgcga gttcttccgc
     1381 caggagatcc gccccgagat cgggatggag gtcaacataa agaaccgcgt gggcaccgtg
     1441 gtcaccgtga ccaacaggat ggtccgcgtg gacttcaacc gccgcttcgc cggctccgcc
     1501 ctgaggtacg agttcaccgt cctggacaag gtcgagggag acgaggcgaa ggtcatggcc
     1561 atctgcgaga tggactacgg caccgccgag ggcttccgcg ccaccgtcgc tgacggaaag
     1621 gtcgttctgt acctgccgga cgtctgcaag tacgatcaga agtggctgct caccaagtac
     1681 aagatcgtcg ccgacctgcg cgaggtcttc ggtccgatcg acgtcgagct cgtcgaggag
     1741 tacctcaaga aggaggacaa gcccgccgag gagaagcccg ctgaggtcgt cgaggagaag
     1801 gccccagagg agctctaaat cctttctttt taaaacattt taaatatctt atttctaatc
     1861 aacggaacac acgggcgggt ggcctagtct ggatatggca gcagcctcct aagctgcagg
     1921 tcgggggttc gaatccctcc ccgctcgctt tctttcatcc accactatcc cgaccttagc
     1981 tattatgatc ggttctccgg ccccggcatc gatgttcgaa tgtgggcgaa cgaatactat
     2041 catgtcatgg ataatcacga caagatcatc gagatgatga tgtcgaccgg gctcaagacc
     2101 tatccggagc acctgaacgc cgaggatgcg tttgagttca taggttctgt gaaggaaggg
     2161 ctgatgagga cccatcctcg tcaaggtgac ggcagcgccc tgttcccatt caagcacctt
     2221 atgatggtgg ccaagaaggt ccaggccatt cactgacggt tgatccatac ctgtgaccgc
     2281 gggacagcga actccatgcc gctctcctcg aacaacttca cc
//
LOCUS       DAOQTR010000377         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_57527_length_2311_cov_4.991135, whole genome shotgun sequence.
ACCESSION   DAOQTR010000377 DAOQTR010000000
VERSION     DAOQTR010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57527_length_2311_cov_4.991135"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..906)
                     /gene="istB"
                     /locus_tag="PKN85_08820"
     CDS             complement(130..906)
                     /gene="istB"
                     /locus_tag="PKN85_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNT44551.1"
                     /translation="MEQLIYERLKGNLARLKLSRMHEVLDTAVSLAERDKLSYCAFLD
                     RLFEEEVAAKEQRRIDMGLRMAGLPSGKTIEEYDFTFHPQLEKKTVMELFDLTFLAKH
                     ENVIFLGPPGVGKTHLATALAVKACGHGFKVFYTTMSALVARLKESQARVKPYLAASL
                     VVVDEVGYLPVNANEAHLFFQFVSSRYEKASTIITSNKSFIDWQELFGDQIIASAILD
                     RLLHHCRVINIKGHSYRLKGKAFAQQLTQRQGGNEAACGS"
     gene            complement(894..2135)
                     /gene="istA"
                     /locus_tag="PKN85_08825"
     CDS             complement(894..2135)
                     /gene="istA"
                     /locus_tag="PKN85_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNT44552.1"
                     /translation="MDIKAMNRNGLSIRRIAKITGLHRKTVKRHLESTSLPEYHRTKR
                     KGSILDPFRPMIDAYLEEDDYQASWIFDKLAGMGYTGGYTVVKDAVRAIKGEKTRIAY
                     IRFETEPAFQAQVDWGDFQIVEPDGTIHAIPAFVMVLGYSRAMYVEFVEKRTLEAFMD
                     CHIRAFDYLGGVPKEILYDCMKHVVIKRHNGRPVFNIEFSRFANHYAFHPRLCPPYAP
                     WVKGKVERPIHYLRERFWRGYSYRCLDRANQDVLTWLSGTANRRQHGTYRQEVSGRWE
                     KEKALLMPLPPSPYDTSLKVFRPVYKECQLSYRGNRYLIPHRFAGKKVMLKIKGKLIR
                     IFHDQDLIASYEEPEARHTLVGDPAIYRLLSQDKEQIRRKYGRQKGRATRGLFTGTLF
                     PEVEIRSLEEYERLAGMSWNS"
BASE COUNT          453 a          658 c          641 g          559 t
ORIGIN      
        1 cttcgggttg atgagctcga gctgcgcgaa gagataatgc ttgcatagat cccttgtagg
       61 tgtcaactaa aaagtgtacc aatttgggcg gagtaaaagt gtaccacccc ccgttatcag
      121 ttttgtccac tatgagccgc atgcggcttc attacctccc tgacgttgtg taagctgctg
      181 agcaaacgcc ttgcctttca gcctgtagct gtgtcccttg atgttgatga ccctgcagtg
      241 atggagtagc cggtccagga tcgccgaggc gatgatctgg tcgccgaaca gctcctgcca
      301 gtcgatgaag ctcttgttcg acgtgatgat cgttgacgcc ttctcgtacc gggaagagac
      361 gaactggaag aagagatgcg cctcattggc attcaccgga agatacccta cctcatcgac
      421 gacaacaagg ctcgcggcca gatacggctt caccctcgcc tggctctcct tcagtctggc
      481 gacaagggcg ctcatggtgg tatagaacac cttgaagccg tggccgcagg ccttgacggc
      541 caaagcggtg gcgagatgcg tcttcccgac acccggaggg cccaaaaaga tcacattctc
      601 atgttttgcc aggaacgtga ggtcaaagag ctccatcacg gtctttttct ccagctgcgg
      661 gtggaaggtg aagtcgtact cttcgatggt cttccccgag ggaagccctg ccatacgcag
      721 acccatgtcg attctccttt gctcctttgc tgcaacctct tcctcaaaga ggcggtcgag
      781 gaacgcgcaa tatgacagct tgtcgcgttc ggcgaggctg acggcggtat cgaggacctc
      841 atgcatgcgg gagagcttca gcctggccag gtttcctttg agccgctcgt agatcagctg
      901 ttccatgaca tacctgccag acgttcgtat tcttccaatg acctgatctc cacctcgggg
      961 aagagcgttc ctgtgaaaag acccctcgtc gcccgtccct tctgtcgtcc gtacttcctc
     1021 ctgatctgct ccttgtcctg tgagagcagg cggtagatgg ccgggtcccc cacaagggta
     1081 tgccttgcct ccggttcctc ataggaggcg atgagatcct ggtcgtggaa gatgcggatg
     1141 agcttcccct tgatcttgag catcaccttc ttccccgcga agcggtgcgg gatcaggtag
     1201 cggttgcccc tgtaggagag ctggcattcc ttgtagaccg gtctgaagac cttcagcgag
     1261 gtgtcgtagg gagaaggagg aagcggcatg agcaacgcct tctccttctc ccaccgcccg
     1321 gatacctcct gccggtaggt accgtgctgc ctcctgttgg ccgtcccgga gagccatgtg
     1381 agcacatcct gatttgccct atcgaggcag cggtaggagt agcccctcca gaagcgctct
     1441 ctcaggtaat ggatcgggcg ttccaccttg cccttcaccc agggggcgta gggagggcag
     1501 agacgggggt ggaatgcgta gtggttggcg aaccgggaga actcgatgtt gaagacgggc
     1561 cgcccgttgt gccgtttgat caccacatgc ttcatgcagt cgtagaggat ctctttcggg
     1621 acgccgccga gatagtcgaa ggcgcggatg tgacagtcca tgaaggcctc gagggtccgc
     1681 ttttccacga actcgacgta catggcccgt gaatacccga ggaccatgac aaaggcaggg
     1741 atggcatgga ttgttccgtc gggctccacg atctggaagt ctccccagtc gacctgggcc
     1801 tggaaggcgg gttccgtttc gaaccggata taggcgatcc tcgttttttc gcccttgatc
     1861 gcccgcacgg catccttgac gaccgtgtag ccgccggtat atcccatgcc ggcaagctta
     1921 tcgaagatcc acgatgcctg gtagtcgtcc tcttccagat atgcatcaat catgggccgg
     1981 aaggggtcca ggatgcttcc ttttcgtttc gtcctgtggt actcgggcaa cgacgtgctt
     2041 tccaggtggc gttttaccgt cttcctgtga agaccggtga tctttgcaat ccgccttatg
     2101 ctcaacccgt tccgattcat cgcttttatg tccataaata cctccctggt aatcactggc
     2161 aacacctcct tgaaagagag tgttaccatg ttttcatctc cagggtggta cacttttcac
     2221 cgccgttttt ggtacccttt tattctgcca tttacagaca gccgatcgcc ttcacctatg
     2281 atgcattaaa ccgtctcata agtaaggtct a
//
LOCUS       DAOQTR010000378         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_57644_length_2308_cov_3.260542, whole genome shotgun sequence.
ACCESSION   DAOQTR010000378 DAOQTR010000000
VERSION     DAOQTR010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57644_length_2308_cov_3.260542"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKN85_08830"
     CDS             complement(<1..525)
                     /locus_tag="PKN85_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44553.1"
                     /translation="MLGIIHRDRRNDELLGKWIDALTPEVITLELSPYGLAFRQAMGK
                     AYRRRIDDIVRSLEGEGHPCLSGEVEDLYAYVDVPREFTIADEYCRRSGARLYPVDMD
                     LFSWMKLRELDELINRTNVEKSVAFRVREGRGAEKVLASLYFRSGVSAFSYTDEMALR
                     DGFMCRGIGALTKRF"
     gene            704..1693
                     /locus_tag="PKN85_08835"
     CDS             704..1693
                     /locus_tag="PKN85_08835"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNT44554.1"
                     /translation="MKTMLCVFCLLFAGQALATDMIIGGDVTHTVGKGDNLYRVGARY
                     GVFWKNIARDNGLDEKQPLVEGTVLRLNTRKIVPRMIDNGIIINIPDRTLYFFQDKQL
                     MALPVGLGAIFENEYSDWKTPEGKFRITEKRKDPTWYVPESIQIEYALKGKPVEESVP
                     PGPKNPLGKYAIKTSLPAVLIHETIWPGSVYRYMSHGCVRMLREHIEQLFPLVQVNMQ
                     GEIIYEPVKIAQTRDGRTYVEIRTDVYRKHRSIRGHLSKLLEARGIADKVDWQKIDKL
                     IKEESGVAEDVSLQPPKGSSLTGTTSKAGFTGRIMDYFKGKFRKTEETSRPRS"
     gene            1833..>2308
                     /locus_tag="PKN85_08840"
     CDS             1833..>2308
                     /locus_tag="PKN85_08840"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44555.1"
                     /translation="MTTRLKKDSPDGRLAAFLTDQRLIAPGELETALEVLKTRGGYLS
                     EVLVGRGLMAEDDVAFALSQEFDVPLLSSQNGSMRPSEDQDLKKLIPKQFALRNVILP
                     FSRDGSVFTCVMFNPLDVMLIDELRKITGCEINTVVATKSDILRAIEEFYDTTVIAP"
BASE COUNT          550 a          693 c          619 g          446 t
ORIGIN      
        1 gaaacgcttt gtaagcgccc cgatgccgcg gcacatgaac ccgtcgcgca gcgccatctc
       61 gtcggtgtat gaaaaagcgc tcactcccga ccggaaataa aggcttgcca ggactttctc
      121 cgccccccga ccctcccgga ccctgaatgc cacgcttttc tccacgtttg tccgattgat
      181 gagctcgtct aactcccgaa gcttcatcca ggagaaaagg tccatgtcga caggatacag
      241 acgcgcgccg ctccgcctgc aatactcatc ggcaatggtg aactcccgag ggacgtcgac
      301 gtaggcataa aggtcttcca cctcgccgga caaacaggga tgcccttcgc cctccaggga
      361 ccgaacaata tcgtcgatcc tcctccggta ggcctttccc atcgcctgcc ggaaggcaag
      421 accgtacggg gacaactcca acgtaatgac ctcgggcgtc agggcatcga tccattttcc
      481 aagaagctca tcgtttcgcc tgtcccggtg gatgatcccc agcatgtaga aggcacaagg
      541 agagacttcg ttatcagggc gcgccatgac taaatttacg cacgaccccg gggacgtttc
      601 aagagaaatg tacaaaccgc cgcgttatca ttctcacttt ttctgttgtc aatttccccg
      661 aggtatacta gaataacctc ctgtcatacc tatgaaaaca tcaatgaaaa cgatgctctg
      721 tgtattctgt ctactctttg ccggccaggc gctggcaaca gacatgatca tcggcggcga
      781 tgtgacgcac acggtgggca aaggcgacaa cctttaccgc gtgggcgcca ggtatggggt
      841 cttctggaag aacatcgccc gggacaacgg actcgacgaa aagcaacccc ttgtggaggg
      901 caccgtcctg agacttaaca cgcgcaagat agtcccgcgt atgatagaca acggcatcat
      961 catcaatata cccgacagga ccctctactt ctttcaggac aaacaactga tggccctgcc
     1021 cgtcgggctc ggagccatct ttgagaatga gtacagcgac tggaagaccc cggagggcaa
     1081 gttccggata acggagaaaa ggaaagatcc gacctggtac gtgccggagt cgatccagat
     1141 agaatacgcg ttgaaaggca aacccgttga ggagtccgtc ccccccggcc cgaagaaccc
     1201 gctcgggaaa tacgccatca agacatcact gccggccgtc ctcattcatg agaccatttg
     1261 gccggggagc gtctacaggt acatgagcca tgggtgcgtc cggatgctca gagaacacat
     1321 agagcagctt ttccccctcg tgcaggtgaa catgcagggg gagatcatct acgaaccggt
     1381 gaagatcgcg caaacacggg acggcaggac ctacgtcgag atccgcaccg acgtctacag
     1441 gaagcatcgt tccataagag ggcatctctc gaagctcctc gaagcccgcg gcatcgcgga
     1501 caaggtggac tggcagaaaa tagacaaact catcaaggag gagtcggggg tggctgagga
     1561 cgtgagcctt caaccgccga aggggtcctc cctgacaggg accacatcga aggccggttt
     1621 cacgggccgc atcatggatt acttcaaggg aaagttcaga aagaccgaag aaaccagccg
     1681 cccgaggtcg tgaaacacgt ctccggtctc aggctgcatg tctcaggttt tgggcctcta
     1741 acccgcaacg cgcaacccgc aacgcggaac ggtcttttca tgaggtctgt tttcggtttg
     1801 ccattattct ccttttttct tacaatagac gcatgacaac aagattgaag aaggactctc
     1861 ccgacgggag gctggccgcc ttcctcacgg accagagact catcgctccg ggtgagctcg
     1921 agacggccct tgaggtcctg aagactcgag gcgggtacct cagcgaggtg ctggtgggcc
     1981 gcgggctcat ggcagaggac gatgtcgcct tcgccctctc ccaggaattc gatgtcccgc
     2041 tcctgtcatc gcagaacggc tccatgaggc cgtcggagga tcaggacctc aagaagctca
     2101 tacccaagca gtttgccctc aggaacgtca tcctcccctt ctcccgcgac ggcagcgtct
     2161 ttacctgcgt catgttcaac cctctcgacg tgatgctcat cgatgagctc cgaaagatca
     2221 ccggctgcga gatcaacacc gtcgtggcga ccaagtccga catactgcgc gccatagagg
     2281 agttctatga tacgacggtg atcgcccc
//
LOCUS       DAOQTR010000379         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_57731_length_2306_cov_2.893825, whole genome shotgun sequence.
ACCESSION   DAOQTR010000379 DAOQTR010000000
VERSION     DAOQTR010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57731_length_2306_cov_2.893825"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKN85_08845"
     CDS             <1..402
                     /locus_tag="PKN85_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44556.1"
                     /translation="SPLFDDIRGGEASHFFPMTRSSVMFQANPLFPRETTFTFMKLLR
                     FMNVVKALIDANPGASALVDLAHADRLKKNPRDLHIFTSLLRERRFLWFDTRGNDYVE
                     EPQDHALVAAFFGALEGMTIKGFRTSNTVRA"
     gene            489..1892
                     /locus_tag="PKN85_08850"
     CDS             489..1892
                     /locus_tag="PKN85_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HNT44557.1"
                     /translation="MRYRKVLGTLVFVVCALVLALVRPFTGLSGEGHYVCSTIFMALG
                     LWIFRPGGVPYLAGSAVLIAGSVLFGVPLGVVTSGFTSSGVWVLIPALYLGYALAKTG
                     LGKRIAYFVLKIFRPGYFTIVLSWFIIGLVLSALTPSITVRLSVVMPIAMNLVEGCGL
                     KDRSRGSALICLAAWGGAVLPGTGWLTGSLWGPFMMGFLPPAMKQFATFDNWFRIMSV
                     PWLIAGVFFVGLVYLLLKPKAPLGVARESFHEAYRALGRISAREIVTAAILTGALALF
                     ATERYHHIPTAACAMMAVLALVLFGIITAEDIGKGANWDVIIFFGAVVSLSGIFAHSG
                     ISAWIRPFIEPGILSLARTPLTFLIAVTVGFWLIRFVDVPWGFSTIALTSSLFIPLFE
                     DYGLHPVLVSVAAIAGGNSFFLAYTQPFIMVSDAITGQRAWSSRHVALAGGLYAVSII
                     AAMLLSSSYWKAIHVIP"
     gene            1959..>2306
                     /locus_tag="PKN85_08855"
     CDS             1959..>2306
                     /locus_tag="PKN85_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44558.1"
                     /translation="MGDLQPRSETRIRIPVDGERSTSGVLLTPGAGGKRTVVIVAHGA
                     GNDMNTPLITSFAEGLCAAGYPALRFNFLYKEEGRSAPDRQEVLVRTWGAVFAHARQL
                     LAGKVDSWVAAGKS"
BASE COUNT          367 a          726 c          689 g          524 t
ORIGIN      
        1 agcccgctgt ttgacgacat ccgcggggga gaggcctcac atttcttccc gatgacgcgc
       61 tcgagcgtca tgtttcaggc gaatcccctc ttcccgcggg agacgacgtt caccttcatg
      121 aagctcctga ggttcatgaa cgtcgtcaag gccctcatcg acgcgaaccc cggagcttcc
      181 gcccttgtcg acctggccca cgcagaccgg ctgaagaaga acccccggga ccttcacata
      241 ttcacctccc ttctaaggga gaggcgcttt ctctggttcg acacccgggg caacgactat
      301 gtcgaagagc cgcaggacca tgccctcgtg gccgccttct ttggagccct ggagggaatg
      361 accatcaagg gatttcgcac atcgaacacc gtgagggcct gacggggcgg ccggggccga
      421 caaaaggcct gcacggcgat cgaaaatgtg gtatcctaac cccttacatc ctgtcgaaag
      481 gaacgcctgt gcggtatcgg aaggttctgg gtacactggt cttcgtcgtt tgcgcgctgg
      541 ttctggccct tgttcgacct ttcaccgggt tgtcgggaga gggacattac gtctgttcga
      601 cgattttcat ggccctcggc ctgtggatct tcagaccggg aggtgtgcct taccttgcag
      661 gatcggccgt cctcatcgcc ggttcggtgc tcttcggcgt gcccctcggg gtcgtgacga
      721 gcggtttcac aagttcgggc gtctgggtgc tcatcccggc cctctatctc ggctacgccc
      781 tggcgaaaac aggcctcggc aagcggatcg cctactttgt gctgaagatc tttcgaccgg
      841 gttatttcac catcgtcctg tcctggttca tcatcgggct cgttctctcc gcgcttacac
      901 cgtcgataac ggtgcgtctt tccgtcgtta tgcccatcgc catgaacctc gtcgaggggt
      961 gcgggttgaa ggacaggtcc cggggctcag ccctcatctg tcttgccgcc tggggagggg
     1021 ccgttctgcc gggtacgggg tggttgacgg ggtccttgtg ggggcctttc atgatgggat
     1081 tccttccccc ggccatgaag cagttcgcca cctttgacaa ctggttccgg atcatgtccg
     1141 tgccatggct catcgccggc gttttcttcg tcggtctcgt gtatctcctt ctgaagccga
     1201 aggcccccct gggggtagcg agggaatctt tccacgaggc ataccgtgcc ctcggcagga
     1261 tcagcgcacg ggagatcgtt accgccgcta tcctcacggg cgccctcgcc ctctttgcca
     1321 ccgagagata tcaccacatt cccacggcgg cgtgcgcgat gatggctgtc ctggcgctcg
     1381 ttctcttcgg catcataacc gcggaggata tcgggaaggg agccaactgg gatgtcatca
     1441 tattcttcgg tgccgttgtt tccctctcag gcatcttcgc gcactcgggc atatccgcgt
     1501 ggatacgacc cttcatagag ccgggaatac tgtccctggc gcggacgccg cttaccttcc
     1561 tgatcgcggt cacggtgggg ttctggttga tccgtttcgt cgacgttccc tgggggttct
     1621 cgacgatcgc gctcacctcc tccctgttca tccccctctt cgaggattac ggtctccatc
     1681 ccgtgcttgt gagcgtcgcc gccatagccg gagggaacag ttttttcctg gcctacacgc
     1741 agcctttcat catggtgagc gacgcgatca ccgggcagag ggcctggagt tcccgccacg
     1801 tggcccttgc cggcggcctg tacgctgtgt ccatcatcgc cgccatgctc ctcagctcct
     1861 cctactggaa ggcgatccac gttatcccct gacgttcctg ccttattccc ctcgaatccc
     1921 ttatgtgata tagttcttcg gatacaggag ggaggacatt gggagacctg cagccacgtt
     1981 cggagacacg gataaggatc cccgttgacg gggagcgctc gacctcgggg gttctcctta
     2041 cgcccggcgc gggagggaag cggacggtcg tcatcgtggc ccacggcgcg gggaacgaca
     2101 tgaacacgcc gcttatcacc tccttcgccg aggggctttg tgccgccggc tatccggccc
     2161 tgaggttcaa tttcctctac aaggaagagg gaaggagtgc gccggaccgc caggaggtcc
     2221 tcgtcaggac gtggggggcg gtatttgccc atgcccggca gctcctcgcc ggaaaggtcg
     2281 attcctgggt ggccgcgggc aaatcc
//
LOCUS       DAOQTR010000380         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_57898_length_2302_cov_2.695594, whole genome shotgun sequence.
ACCESSION   DAOQTR010000380 DAOQTR010000000
VERSION     DAOQTR010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57898_length_2302_cov_2.695594"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKN85_08860"
     CDS             complement(<1..477)
                     /locus_tag="PKN85_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT44559.1"
                     /translation="MVPGTHHPKARMSRKPVRKNEEQNASSWKPIEYQGDLYAKDRLL
                     TSVHKISHLLTRPVSLDTVLTAIVEETAHVFGYDRVAIYLVSKDKRLLECKYEIGFTP
                     EEMEIAFQRPFNMERHECIETLVVKTGHTVYIRDYNTDSRVTYLDRKVSAKQNRFAT"
     gene            complement(759..1112)
                     /locus_tag="PKN85_08865"
     CDS             complement(759..1112)
                     /locus_tag="PKN85_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44560.1"
                     /translation="MKAPNKTATIYQWVADPTQTCLGKTRARVAEVMTVDAIPGRVFV
                     PAGGVPARVRRISERLIKYEKRGSVGIITARVEEIGEIYDELDTLLDRIEGDKEIQVV
                     AIYTPSGLFASLPIR"
     gene            1437..1757
                     /locus_tag="PKN85_08870"
     CDS             1437..1757
                     /locus_tag="PKN85_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44561.1"
                     /translation="MKKILQNRIDAIDGLKGKTAASGEFIQWRRQTEGTLKALYGEES
                     TEVQDFNAIYYSPVFLTCRMSDEAFLESYRNGLEEARRLLQACMKTLRQLDGLTSCER
                     TPRG"
     gene            complement(1708..>2302)
                     /locus_tag="PKN85_08875"
     CDS             complement(1708..>2302)
                     /locus_tag="PKN85_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44562.1"
                     /translation="GLNPVKATLFRVVAAVATVWAMTLFIETDPTASAQAGALAASTQ
                     AFHAMVYDWAVSMLTLSVKIFFIIMAILTLLDIAKSMGWVMPIVRALSPFLRVLGLSE
                     KVGILWMTAVIFGLTYGGVVIVEEARQGHISKEELEELQLSIGINHSMVEDPVLFLAF
                     GISAFWLWVPRLITAMAATRMLTLWVSFRKTSARQAA"
BASE COUNT          447 a          660 c          706 g          489 t
ORIGIN      
        1 cgtcgcgaag cggttctgct tcgcgctgac cttccggtcc aggtaggtca cccggctgtc
       61 ggtgttgtaa tcgcggatgt agacggtatg ccccgtcttg accacgaggg tctcgatgca
      121 ctcgtggcgc tccatgttga aggggcgctg gaaggcgatt tccatctcct cgggcgtaaa
      181 gccgatctcg tacttgcact cgagaagccg cttgtccttg ctgaccaggt agatcgcgac
      241 gcggtcgtag ccgaagacat gggcggtttc ttcgacgatg gcagtcagga cggtgtcgag
      301 ggaaacgggt cgggtgagga gatggctgat cttgtggacg gaggttagca gcctgtcctt
      361 ggcgtaaaga tcgccctggt attctatggg tttccaggac gatgcattct gctcttcgtt
      421 tttccttacg ggtttgcggc tcatgcgtgc cttggggtgg tgggttcccg gcaccacgca
      481 cggggaatca tacgccattt gtccaaattg gcaaggtttt tttcctgccc ccaccctcgc
      541 ggcactgaaa ccggcccgga cgggatgctt ccggccgggc accccctact gaatcatctg
      601 gctccgccgt gcggcagcct ttcgatctct tttcctgtct gcttgagctt tttcactctt
      661 cttgctgccg tgaaccagct cgggaaatcc tccggcatgg gagcaacgcc tcctcgatgc
      721 ccatggcgtt cactcggggc gaggccctct gtctgcactc accgaatggg aagcgacgca
      781 aagagcccgc tcggggtgta gatggcgacg acctggatct ccttgtcccc ttcgatccgg
      841 tccagcaggg tatcgagttc atcgtagatc tcgccgattt cttccacacg cgccgtgatg
      901 atgccaacgg atccccgctt ctcgtacttg atgagtcgct cggaaatcct gcgcaccctt
      961 gccgggacac cgcctgcggg gacgaatacc ctgccgggga ttgcatcgac agtcatgacc
     1021 tctgccacgc gggctctggt cttgccgagg caggtctggg tcgggtcggc cacccactgg
     1081 taaatcgttg cggttttgtt cggcgctttc atgatgtcgc ctcctctttc ttcaatgtgt
     1141 ccgacctcct gtagagcaag tgccgtgccc gggtggatga atttgtaata cgctgatatc
     1201 gttggatttt ttaaagggac attcccggca tatcccggaa aaaatgaagc acatcctctg
     1261 ccagcctggg acgcgtggag taaccgagat aaattgcaag tattttcagg gagttgaaga
     1321 aacgaagcat ttgcttcgca ggggaagcag atgcttcgtt cgtggaacgc ctgtccccgg
     1381 gctacgtgat gtggtatgaa tgaaacaaac agcaggtgac aggcaacagg tgtcgggtga
     1441 aaaaaatact tcaaaatcga atagatgcca ttgatgggct gaaggggaaa acggccgcgt
     1501 caggagagtt catccaatgg cgcaggcaga cggaggggac cctgaaagcc ctctacgggg
     1561 aggagtccac cgaggtccag gatttcaacg cgatctatta ctcgcccgtt ttcctcacct
     1621 gccggatgag tgacgaggcc ttcctcgaat cctatcgaaa cggcctcgag gaagcccggc
     1681 gccttctgca ggcttgcatg aagacactca ggcagcttga cgggctgacg tcttgcgaaa
     1741 ggacacccag agggtgagca tccgggtggc cgccatggcc gtgatgagac gcggcaccca
     1801 gagccagaag gcgctgatcc cgaaggccag gaacaggacc gggtcctcca ccatggagtg
     1861 gttgatcccg atggagagct gcagctcctc gagctcctcc tttgatatgt gcccctgccg
     1921 ggcttcctcc acgatcacga ccccgccgta ggtcaggccg aagatgacgg ccgtcatcca
     1981 caggatcccc accttttcgc tcaggcccag gacgcgcagg aaaggcgaaa gggccctcac
     2041 gatgggcatg acccacccca tgctcttggc gatgtccagc agggtgagga tcgccatgat
     2101 gatgaagaag atcttcaccg agagtgtcag catcgagacg gcccagtcgt agaccatggc
     2161 gtggaaggcc tgcgtggagg cggccagcgc ccccgcctgc gccgacgcgg tgggatcggt
     2221 ttcgatgaaa agggtcatgg cccagacggt ggccacggcg gcaacgaccc ggaacagcgt
     2281 cgccttgacg gggttgaggc cg
//
LOCUS       DAOQTR010000381         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_58569_length_2285_cov_2.414798, whole genome shotgun sequence.
ACCESSION   DAOQTR010000381 DAOQTR010000000
VERSION     DAOQTR010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58569_length_2285_cov_2.414798"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKN85_08880"
     CDS             <1..262
                     /locus_tag="PKN85_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HNT44563.1"
                     /translation="QTILKEITNEDLSLALKTASEALKELILRNMSSRASEMLLEDME
                     AMGPVKLSDVENAQQTIVRVVRKLEAAGKIVIGGKGGDDVLI"
     gene            249..905
                     /locus_tag="PKN85_08885"
     CDS             249..905
                     /locus_tag="PKN85_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNT44564.1"
                     /translation="MSSSKILKGGGTKSRPVSAYTVEDMATKGKLSKVEEAQREAEAI
                     VRQAKTRKEAIEMEAYNQGMAKGQEEGRKIVTKKIEPLFDTLKNAIDEIAHIRLSLVE
                     KHNKQILEMIFLIIEKIIHRSIQISPDILLETIRAASVHLMETDEIHLRLHPSDFEYI
                     REIETILSKKLSGKKSFHIVEDSSLERGGVIIETEFGEIDASIRSQIDRVKEAVLDHD
                     "
     gene            898..2217
                     /locus_tag="PKN85_08890"
     CDS             898..2217
                     /locus_tag="PKN85_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNT44565.1"
                     /translation="MIDFAKHIASLRDTDPIEVRGKVTNIVGLVVEGHGPGSCMGGMC
                     EIYSKGVSGSIMAEVVGFRDKRVLLMPLGDLGGIGPGSTIIARKSNPSAKVGDELLGR
                     VIDGMGNPLDGKGPLSLGQEMPLYGRTVNPLKKKRITEPLDLGIAAINGVLTIGKGQR
                     MAIMAGSGVGKSVLMGMMARHTQADVNVIALIGERGREVKEFIERDLGPEGLKRSVVI
                     AAISDQPALIRIRGAYLATTVAEYFRDKGLDVLLMMDSITRYALSMREIGLAVGEPPT
                     TKGYTPSCFAKLPRLLERAGTTSSKGSITGLYTVLVEGDDFSEPVADSVRSIVDGHIV
                     LSRELAARNHYPAIDILGSVSRVMNDIVSREHMKMARRIVEYKAVYEEARDLINIGAY
                     VAGSNPQIDTAVKHIDAVNEFLRQDIDERVTLEESLARMGKIIPAVK"
BASE COUNT          574 a          605 c          700 g          406 t
ORIGIN      
        1 acagaccatc ctcaaggaga tcaccaacga agacctctcg cttgcactca agaccgccag
       61 cgaggcattg aaagagctga tattgaggaa catgtcctcc cgcgcatccg agatgctcct
      121 ggaagacatg gaggcgatgg gcccggtgaa gctctcggac gtggagaacg cacagcagac
      181 cattgtgcgc gtggtgagaa agctcgaagc ggccggcaag atcgtcatcg gcggaaaggg
      241 cggcgacgat gtcctcatct aagatcctca agggcggggg taccaagagc cgtcccgtta
      301 gcgcctatac cgttgaggat atggcgacaa aggggaagct cagcaaagtg gaagaggcgc
      361 agagagaggc cgaggccatc gtgagacagg caaagacccg caaagaggcc atcgagatgg
      421 aggcgtacaa ccagggaatg gcaaagggac aggaggaggg ccggaaaatc gtcaccaaga
      481 agatcgagcc cctgttcgac accctgaaaa acgccataga cgagattgct cacataaggc
      541 tctctcttgt cgaaaagcac aataaacaga tccttgaaat gatctttctc attatagaga
      601 agatcattca tcggagcatc cagatctctc ccgacatcct gctggaaaca atacgggccg
      661 cgagcgtcca cctcatggaa accgatgaga tccatctccg cctccatcct tcggacttcg
      721 agtacatacg agaaatagaa accattcttt ccaagaaact ctctgggaag aaaagcttcc
      781 acatcgtcga agacagctcc ctggagaggg gaggcgtcat catcgaaaca gaattcggcg
      841 aaatcgatgc atccataagg tcgcagatcg atcgggtgaa agaagcggtt ctggaccatg
      901 attgattttg ccaagcacat cgcatctctc agagacaccg accccattga ggttcgggga
      961 aaggtcacga acatcgtagg actcgtggtg gagggccacg gaccgggcag ctgcatggga
     1021 gggatgtgcg aaatctactc caaaggagtg agcggctcga tcatggccga ggtcgtaggt
     1081 ttcagggaca agcgggttct gttgatgcct ctgggtgatc tgggcggcat cgggccggga
     1141 agcacgatta tcgccagaaa gtcaaatccc tctgccaagg tgggcgatga gctcctggga
     1201 cgggtgatcg acggcatggg caatccactg gacgggaagg gacctctgag cctcggacag
     1261 gaaatgccgc tctacggccg gaccgtcaat cccttgaaga aaaagcggat tactgagccg
     1321 ctcgatctgg gcattgcggc gatcaatgga gtccttacca tcgggaaagg ccagcgcatg
     1381 gcgatcatgg cgggctccgg ggtggggaag agcgtgctca tgggtatgat ggcccgccac
     1441 acacaggcgg atgtcaatgt gatcgccctg atcggcgagc gcggcagaga ggtcaaggag
     1501 ttcatcgaaa gagacctggg acccgagggg ctcaaacgat ccgtggtaat cgccgccatc
     1561 tcggaccagc ctgcgctgat ccgcatccgg ggagcgtatc tcgccaccac tgtggcggaa
     1621 tatttccggg acaagggcct cgatgtgttg ctcatgatgg attccataac ccggtatgcc
     1681 ctttccatgc gggagatcgg cctggcagtc ggagagcctc ccaccaccaa gggatacacc
     1741 ccctcgtgtt tcgccaagct ccccaggctc cttgagaggg ccgggaccac ctcgtcgaag
     1801 ggaagcatca cgggtctcta caccgtactc gttgaggggg acgacttttc tgagccggtg
     1861 gccgacagcg tccggtccat tgtcgacggt catatcgttc tctcgcggga gctcgcggca
     1921 aggaaccact accccgccat agacatcctg ggcagcgtga gcagagtgat gaacgacatc
     1981 gtgagcagag agcacatgaa aatggcgcgc aggatcgtgg agtacaaggc cgtctacgaa
     2041 gaggcacggg acctcattaa tatcggcgcc tatgtggcgg ggagcaatcc gcagatcgat
     2101 accgccgtca agcacatcga tgccgtcaat gaattcttga ggcaggatat cgatgaacgg
     2161 gtgaccctgg aagaatctct cgccaggatg gggaaaatca tccccgccgt gaagtgaaga
     2221 aggacgcgct atgggattcc gtttccggtt cgagaccttg ctgagagtca ggaagatccg
     2281 tgagg
//
LOCUS       DAOQTR010000382         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_58947_length_2275_cov_4.737387, whole genome shotgun sequence.
ACCESSION   DAOQTR010000382 DAOQTR010000000
VERSION     DAOQTR010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58947_length_2275_cov_4.737387"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1527)
                     /locus_tag="PKN85_08895"
     CDS             complement(238..1527)
                     /locus_tag="PKN85_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT44566.1"
                     /translation="MKDIYKFLILLGAFVGAYFIPFDNPSVRKAILESFYMLQDYAQK
                     HVLLCLVPAFFIAGAISVFISQASVMKYLGPAANKCLAYSVASVSGTVLAVCSCTVLP
                     LFAGIYRMGAGVGPATSFLYSGPAINVLAIVLSAKVLGWKIGLARAVGAVVFAYVIGL
                     LMAVTFRKEEGTRKAVCMPEDEPKRKLWQDVAYMASMIGILVFANWGKPVDESGIWYT
                     IHASKWYIAGLFALLFGYAIVRWFGAKLIPTMITGVVVLILALLFPEWPLLAFSAGIA
                     GVVWVTKISSDETRSWIDSTWMYALMIAPLLFGGVVVAGFLLGMPGTDNGIIPSRWVS
                     AMVGGNGLFANLFGSIVGAFMYFATLTEVPIVQGLLGSGMGQGPALALLLAGPALSLP
                     SMIVIRTVLGTKKTLVFVAYVIILSTIAGMLYGWMVG"
     gene            complement(1553..1873)
                     /locus_tag="PKN85_08900"
     CDS             complement(1553..1873)
                     /locus_tag="PKN85_08900"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT44567.1"
                     /translation="MITMEEAEIRSNIIKAMGHPVRLMMIDVLKDGTRSFSEIFSLFT
                     LDKSTVSKHLLVLKEAGIVTSQKTGADMIYRLEVPCVTDFFGCVTAVIENNVRRQQVC
                     LCKR"
     gene            2020..>2275
                     /locus_tag="PKN85_08905"
     CDS             2020..>2275
                     /locus_tag="PKN85_08905"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT44568.1"
                     /translation="MEDIAQIYKALSEQMRLRILMLLTHGELCVCDLMAVLEEPQSKV
                     SRHVAYLKNSGLIKGRRVGTWMHYFLKEPLEGLVAAHVEFL"
BASE COUNT          516 a          627 c          651 g          481 t
ORIGIN      
        1 caccgtcgat gacgagggcg ggggtcacca tcacgccgta gttcataatg tcgttgatac
       61 cggtgacctt gccgatctcg aactcgatgc ccgcgccttg ggccgcctcc cgggcgtttt
      121 cggcaagctt cacgcacttg gcgcatccgg ttcccaggat ctcgatcttc ttcatgttgc
      181 tcacctcccg atgggttcat tttccaatgc gcgtaacgca tggcgcgcct tgcgacgcta
      241 cccgaccatc catccgtaca gcatgcccgc gatggtggaa agaatgatca cataggcgac
      301 gaatacaagg gtctttttcg tgccgaggac agttctgatg actatcatgc tgggcagcga
      361 caatgccggt cccgcaagga ggagggcgag ggcggggccc tgccccatgc ctgagcccaa
      421 tagaccctgc actatgggta cctcggtcag tgtcgcgaaa tacatgaacg cacccacgat
      481 ggacccgaaa aggttggcga aaagaccgtt gcctccgacc atcgcggaga cccaccggga
      541 cggtatgatc ccgttgtccg ttcccggcat cccgaggaga aacccggcga caacaacgcc
      601 gccgaaaagg aggggggcga tcatgagggc gtacatccac gtggaatcta tccagcttcg
      661 agtctcgtcg gaggagatct ttgtcaccca caccacaccg gcgatacccg ccgaaaaagc
      721 aagaagcggc cattctggga agagaagggc caggatgaga acgacaacgc ccgttatcat
      781 ggtcgggata agcttcgctc cgaaccatct gacgatggca tacccgaaga gaagagcgaa
      841 gaggccggcg atataccatt tggaggcatg gatggtgtac cagatgccgc tttcatccac
      901 cggttttccc cagttggcga agacgaggat gcctatcatg gaggccatat atgcgacatc
      961 ctgccagagc tttcttttcg gttcatcctc gggcatgcac accgccttcc tggtgccctc
     1021 ctctttgcgg aaagtgaccg ccatgagaag ccctataacg tacgcgaaga cgactgctcc
     1081 cacagctcgc gcaaggccaa tcttccatcc caggaccttg gcggacagga cgatggcgag
     1141 gacattgatg gccggccctg aatacaggaa agacgtggcc ggccccacgc ctgcacccat
     1201 ccggtatatg ccggcgaaaa ggggcagaac ggtgcaggag cagacggcga gcacggtacc
     1261 agaaacggat gctacggaat aggcaagaca cttgttcgcc gccgggccaa ggtacttcat
     1321 gaccgatgcc tgagagatga agacggatat ggctccggca atgaagaagg ctgggaccag
     1381 acagagaagc acgtgtttct gcgcgtagtc ctgaagcatg tagaaggatt ccaggatggc
     1441 cttccggacc gagggattgt cgaatggtat gaaataggct ccgacaaagg ccccgagcag
     1501 tatcaggaac ttgtatatgt ctttcatcgt gtggacctct tcgctcccgc agctacctct
     1561 tgcacaggca tacctgctgc ctgcgaacat tgttttcgat aacggcggtg acgcacccga
     1621 agaaatcggt cacgcagggc acctccagcc tgtatatcat gtccgcgccg gtcttttgag
     1681 acgtgacgat gccggcttct ttgagcacca gcaggtgctt tgagacggtg gatttgtcca
     1741 gcgtgaagag gctgaatatc tcggagaagg accgtgtgcc gtctttcagg acatctatca
     1801 tcatgagtct caccgggtgc cccatggctt tgatgatgtt gcttcgtatt tccgcttctt
     1861 ccatggttat catagcgtct cctcttgcct tgttggtaac aataccaaca aggcaacgat
     1921 tagtcaaggg atttcctgcc cggttttctt gttgacagcg ggagagtttt agtctttatt
     1981 cttatatccg tttaagcaga tagattcggg cgatgaggga tggaagacat agcgcagata
     2041 tacaaggcac tctccgagca gatgcgattg cgcatactca tgcttctcac ccatggggag
     2101 ctctgtgtgt gcgacctgat ggcggtcctc gaggaaccgc agtcaaaggt gtctcgtcac
     2161 gtggcgtacc ttaagaattc gggactcata aagggcagga gagtgggcac gtggatgcac
     2221 tacttcctga aggaacccct tgaagggttg gtcgcggccc atgtcgagtt cctta
//
LOCUS       DAOQTR010000383         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_59077_length_2272_cov_3.080740, whole genome shotgun sequence.
ACCESSION   DAOQTR010000383 DAOQTR010000000
VERSION     DAOQTR010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59077_length_2272_cov_3.080740"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKN85_08910"
     CDS             complement(<1..516)
                     /locus_tag="PKN85_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44569.1"
                     /translation="MDARSRKIGSFFIIGVAMFFLSCIVLETAAEARAGGGRSMGSRG
                     FSSGSRSSGGSYNRSAPYNTTNPTVQPGTPRQPNPYSPGRSFLYGLGGGLMGGMIGSM
                     LFGRSGYGGMGNYGGGGFGFGDIVILLIIAGIIYFVYKRYKANRQMQMSGASPAGFGS
                     YGYNEPVYDISY"
     gene            complement(671..1321)
                     /locus_tag="PKN85_08915"
     CDS             complement(671..1321)
                     /locus_tag="PKN85_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNT44570.1"
                     /translation="MSVYLASVIFVVLAEMGDKTQLLAMAFASRYRWQTVLWAVFVAT
                     LVNHFFAVVAGSYLTRFVPIQYIQIAASASFILFGLWTIRGDTLEGEDKRFNFSPFWT
                     VAVAFFFAEMGDKTQLATVALATKYSNILVVWFGTTTAMIIADAIGIIIGIVMGKKIP
                     ERFVKWFAAVIFMLFGAIGLYEYLPKNLLTPPVIAAGLALIVLLTILVARRNNAKA"
     gene            1741..>2272
                     /locus_tag="PKN85_08920"
     CDS             1741..>2272
                     /locus_tag="PKN85_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44571.1"
                     /translation="MTHAFKVLVTDDDPIVLELTSSLLRQAGYEVLQATTGQECLETV
                     RTHHPDLIILDVMLPDVTGTDICRKVKNDPGLQGSFVILLSGVHISSNQQAEGLSAGA
                     DGYLVKPVSRKEFIARVQAMERIKRAEDALRETKKEQEATIQELRKALEEIKTLRGLI
                     PICAWCKRIRDDAGYWD"
BASE COUNT          506 a          641 c          684 g          441 t
ORIGIN      
        1 gtatgagatg tcatagaccg gctcgttgta tccgtaagag ccgaagcccg ccggggaagc
       61 gccgctcatc tgcatctgtc tgttggcctt gtagcgtttg tacacgaagt agatgatccc
      121 ggctatgatc aggaggatga cgatatctcc gaaaccgaac cctccgccgc catagttgcc
      181 cattccgccg taaccggacc ggccgaagag catgctgccg atcattccgc ccatgagtcc
      241 gccgccgagg ccgtaaagaa aactcctccc cgggctgtac ggattcggct gccggggagt
      301 gcctggctgg acggtcgggt tggtggtgtt atagggtgcg gaccggttgt aagagccacc
      361 cgagctcctg cttcccgaac tgaatccccg ggaccccata ctgcgtccgc ctccggcccg
      421 tgcctcggcc gccgtttcga ggacaatgca ggaaagaaag aacatcgcca cgccgataat
      481 gaagaagctc cctatttttc ttgaccttgc gtccatgttt ttccctttac ccccaattat
      541 tgatagaacc gcttgaagtg tcaggatatc atatagtttt tcgggatgtc aaagcgtacc
      601 gcccgtcggg cgaacgagca cacgggtcag cgctcccgcg cggactcgca ggcgcaaagc
      661 gccgacccgt tcacgctttc gcattgttcc tgcgggctac gaggatggtc agaaggacga
      721 tgagcgccag ccccgccgct attacgggtg gcgtgagcag gttcttcggc agatactcat
      781 agaggccgat ggcgccgaag agcatgaaga tgacggcggc gaaccatttg acaaagcgtt
      841 cggggatctt cttgcccatg acgatcccga tgatgatgcc gatggcgtcg gcgatgatca
      901 tcgccgttgt ggtaccaaac cagaccacga ggatattgct gtactttgtt gccagggcca
      961 cggtggcgag ctgggtcttg tcccccatct cggcgaagaa gaaggccacg gccacggtcc
     1021 agaaggggct gaagttgaac cgcttgtcct ccccctccag cgtgtccccg cggatggtcc
     1081 agagaccgaa gagtatgaac gaggcggagg ccgctatctg aatgtactgt atggggacga
     1141 accgcgtgag atagcttccg gccacgacgg cgaagaaatg gttgacgagg gtggcgacga
     1201 agactgccca cagcaccgtc tgccagcggt accgcgacgc gaaggccatc gcgagaagct
     1261 gggtcttgtc ccccatctcg gcgagaacta cgaagatgac cgatgccaga tatacgctca
     1321 ttcgaacccc cgaagtgcca aaaaaaacga gactcttcag tacgcggtac gatcgcatac
     1381 cgaaggtctc gctaattggc ttatgcctgc caacggcagg accagggtcg ccccagcatg
     1441 ttgatcctgc ctgtctagct actccccttc aacaacgcgg gactattata agccggaaaa
     1501 acgtcaaaaa tcaagaattg cccatggaaa gaagcgcgtg caccggcggg cagggtgccc
     1561 gcctgcacgt tggcgggatt tgccgtacgg gcgattcact cacggtcggc tccgcgcgtg
     1621 tcaaggcaac atggtgccgg ctggaactta ctgaaaggac aatgggtttt tcgggggtcc
     1681 atcggttgac gtccccgtgt atctgtgatg gaatatagga caggtgagga ggtggacagc
     1741 gtgacccacg cattcaaggt acttgtcacg gacgatgatc ccattgtcct cgaactgacg
     1801 tcctccctcc tccggcaggc gggatatgag gtgttgcagg ccacgacggg acaggagtgc
     1861 ctcgagacgg tccgtaccca tcatccggac ctcatcatcc tcgacgtcat gcttccggat
     1921 gtgacgggga cagacatatg ccggaaggtc aagaatgatc ccggcctgca gggttccttc
     1981 gtgatccttc tttccggagt ccatatatcg tcgaaccagc aggcagaggg tctttcagca
     2041 ggtgccgacg gttatctcgt caagccggtc tccaggaagg agttcattgc ccgggtacag
     2101 gcgatggaac ggatcaagcg tgcagaggac gcgctcaggg aaacgaagaa ggagcaggaa
     2161 gcaacgatcc aggagcttag aaaagccctc gaagagatca agacgctcag aggtctcata
     2221 ccgatctgcg cgtggtgcaa aaggatacgg gacgacgcgg gatactggga cg
//
LOCUS       DAOQTR010000384         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_59452_length_2263_cov_3.334239, whole genome shotgun sequence.
ACCESSION   DAOQTR010000384 DAOQTR010000000
VERSION     DAOQTR010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59452_length_2263_cov_3.334239"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKN85_08925"
     CDS             <1..978
                     /locus_tag="PKN85_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT44572.1"
                     /translation="PKLFINVDRDKASAMGLSLDQIQNTFFSAYASRKVSTIYGKLNQ
                     YAVILEVQPQFKSDPDALSYLYVKSTANKLVPIGTISTIEQTVGPVNVNHTGQLNSAT
                     IAFNLTPGHSLGEAVDATHRIAKDVNLPQSIVTGFQGSAQEFQRSFSGMGFLLFVTVL
                     VIYMVLGILYESFIHPITILTALPLAACGALLTLWVFGMELDMYGMVGIIMLIGIAKK
                     NGIMMVDFALEEERTKGLNSEESIYQACLIRFRPIMMTTMAALFGTLPIALGIGAGGD
                     ARQPMGVAVVGGLFLSQIMTLYITPVFYIYFDELNRWLGKRSGRKEKTV"
     gene            990..1715
                     /locus_tag="PKN85_08930"
     CDS             990..1715
                     /locus_tag="PKN85_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT44573.1"
                     /translation="MSQVYKGVQFIPGQDEFIPDSHVYVIGDPASGDISIVDAGLTGK
                     GAYKAASIAKLGIRPDDIKRVIMTHTHLDHIGCLAELQKAFPVAELWVHGLEADLLEK
                     GDDRAVYGMDMFKGMCQMQFGLAPDAFKFKVDRKLEGGETLEIGNMVWDVIHIPGHSM
                     GGIALYEPIGKILIPGDVVYADYAIGRFDLFGANPAQLKDSLNTLSKLDVDILLPGHN
                     QIVKGVPADYIRKTTKMWEPYLV"
     gene            complement(1807..>2263)
                     /locus_tag="PKN85_08935"
     CDS             complement(1807..>2263)
                     /locus_tag="PKN85_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNT44574.1"
                     /translation="VTMNTLGSPIAGRTQVSVGFEPKTLALLKELKEEAEKTERTIEE
                     IRKHISTLEEMSLAGRLPDEKERLYGRLLATADELEHQLQEQRGRVEMIESTMTKAAQ
                     PTVRVRGACYPNARIKIGTQVFDCTTEYHSAVFYEEEGRIKVNVYDNKT"
BASE COUNT          454 a          667 c          627 g          515 t
ORIGIN      
        1 ccgaaactct tcatcaatgt ggaccgcgac aaggcgtcgg cgatgggcct cagcctcgac
       61 cagatccaga acaccttctt ctccgcctac gcgtcacgga aggtatcgac catctacggg
      121 aagctcaacc agtatgccgt catcctcgag gttcagccgc aattcaaatc ggatcccgac
      181 gcgctttcct atctttacgt caaatcgacg gcgaacaagc tcgtgcccat aggtaccata
      241 tcgaccatcg agcagacggt gggccccgtg aacgtcaatc atacgggcca gctcaactcg
      301 gccaccatag ccttcaatct caccccggga cactccctgg gtgaggcggt ggatgccacg
      361 cacaggatag cgaaggatgt gaatctgccg cagtccatcg tgacgggctt ccaggggtcg
      421 gcgcaggagt tccagagatc cttttcgggc atgggtttcc tcctcttcgt cacggtgctc
      481 gtcatctaca tggtcctcgg catactctac gagagcttca tccatcccat cacgatcctg
      541 acggccctgc cgctggcggc gtgcggcgcc ctgctgaccc tgtgggtttt tggtatggaa
      601 ctcgacatgt acggtatggt aggtataata atgctgatcg gcatcgcaaa gaagaacggc
      661 atcatgatgg tcgacttcgc cctcgaggag gagcggacga aagggctcaa ctccgaggaa
      721 tcgatctacc aggcgtgcct catacggttc cggcccatca tgatgacgac gatggcggcc
      781 cttttcggca ccctgcccat cgcgctcggg atcggagcgg ggggggacgc gcgtcagccc
      841 atgggtgtcg ccgtggtggg cggtctcttc ctttctcaga tcatgaccct ctacatcacg
      901 cccgtcttct acatctattt tgacgagctc aaccgctggc tcggaaagcg gtccggcagg
      961 aaagaaaaaa cggtataaag gagcctctca tgtcccaggt ctacaaaggt gttcagttca
     1021 ttcccggtca ggacgagttc atccctgatt cccatgtcta cgtcatcgga gacccggcat
     1081 ccggggacat ttccatagtg gacgcggggc tcacgggaaa gggcgcctac aaggccgctt
     1141 ccatcgcgaa gctagggatc agacctgacg atatcaagag ggttatcatg acccatacgc
     1201 acctagatca catcggctgc ctcgcggagc ttcagaaggc gttccccgtc gccgagctgt
     1261 gggtccacgg gctggaggcc gatctcctgg aaaaagggga cgaccgggcc gtatacggca
     1321 tggacatgtt caaggggatg tgccagatgc agttcggtct cgcgcccgat gccttcaaat
     1381 tcaaggtcga ccgcaagctc gaagggggcg agacgctgga gataggcaac atggtgtggg
     1441 acgtgataca catacccggc cattccatgg gagggatcgc tctctatgag cccatcggga
     1501 agatccttat ccccggggat gtggtctacg ccgactacgc catcggccgc ttcgatcttt
     1561 ttggcgcgaa tccggcccag ttgaaggact cgctcaacac gctgtcaaag ctcgacgtcg
     1621 acatcctcct gcccggccac aatcagatcg tgaagggcgt ccccgccgac tacatacgaa
     1681 agaccacgaa gatgtgggaa ccatatctcg tttaaaagaa ggttcaaggg ttcaggagaa
     1741 aggcaccgtg cctgcggtgt acgtgtgcag attgtgtttt ttttctcttg aactcttgaa
     1801 cgttttttac gtcttgttat cgtagacgtt caccttgatc ctgccttctt cttcataaaa
     1861 gacggcgctg tggtattctg tcgtgcagtc gaagacctgt gtgcctatct ttatcctggc
     1921 attggggtag caggcacccc gcaccctgac ggtcggctgc gcggcctttg tcattgttga
     1981 ctctatcatt tcgacccggc ccctctgttc ctgcaactgg tgctccagtt cgtccgctgt
     2041 cgcgaggagc ctgccataga gtctctcctt ttcatccggg agtcgaccgg cgagactcat
     2101 ttcttcaagg gttgatatgt gtttccggat ctcctctatg gtgcgctccg tcttttcggc
     2161 ctcttccttc aattccttca ggagggccag cgtcttcggt tcgaacccca ccgatacctg
     2221 tgtcctgccg gcgatcgggg accccagggt attcatggtg aca
//
LOCUS       DAOQTR010000385         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_59567_length_2261_cov_2.669084, whole genome shotgun sequence.
ACCESSION   DAOQTR010000385 DAOQTR010000000
VERSION     DAOQTR010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59567_length_2261_cov_2.669084"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..798)
                     /locus_tag="PKN85_08940"
     CDS             complement(301..798)
                     /locus_tag="PKN85_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44575.1"
                     /translation="MTVKVYMRTVLLAALVTMSLGGFMLHLRVHPFSQHSSILVNIVA
                     SLLSILVVPVLFSIRKTVHYGYVLNGFITIVATITMGHFSIHYLPDPLSIGGIFLGTT
                     LPDILIVWGKFFVGKALFDLEVMGYNADVEKKGIAWRYPNLGWWLVHLIAVSVVYWAG
                     NVLWR"
     gene            1027..1653
                     /locus_tag="PKN85_08945"
     CDS             1027..1653
                     /locus_tag="PKN85_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44576.1"
                     /translation="MSYLLLAVTFIISLLLPASSLHAGPVVAVKDFQGSGPSSQLGSG
                     LSSMVETELANSEGLKKCGGSVVEWKRRKDVVDEIERQQGPGFDPKSRAKKGKLLEPT
                     VFVVGDVITSDTDVRWSIKLVDPATGRTIGRHDGSVPADKDSVFEAPREIAENLGEQI
                     CRQGMEYTSPAGRSGNAGTSDSPAGDPSGVLDKVNGVVDSIKGFKKLF"
     gene            1663..>2261
                     /locus_tag="PKN85_08950"
     CDS             1663..>2261
                     /locus_tag="PKN85_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44577.1"
                     /translation="MRSACSTGTTGWIPRSVPLSWQVRPPLLIVSVTLLVYLNSLSGV
                     FQFDDYNVIVNNPAVHGWSQWWRGVGGIRPLLKLSYTLNWTSGFGLIGFHLFNVLVHV
                     VNALLVYRLAQAVGRRSFRPGSAEKAAFTGALLFAIHPVQTEAITYISGRSSSLAACF
                     YLASLLCYLQGHRRDGPTWPQALSAIFFILALLTKEAAVT"
BASE COUNT          486 a          664 c          646 g          465 t
ORIGIN      
        1 tatgaagacg catgccatgc acatggctga ctggcacccg gacgaccccg actgaataac
       61 gccgcggccc tttccatctg ccccttcgcg agccctgcgc ccccattgcc tggcgcaaga
      121 cgcacgatca ggaaagattc gccggagggt cgctctgccc cgcgtgtcac gaatgtgaga
      181 atgagaaggt gtcccaagct gtttcagaac gtggtggcat ggccgagcca caggaaaaag
      241 gcgacggcta tgacgtgaag tatccaccag ccgagggaaa aagccctgaa agcgttcatg
      301 tcatctccac agcacgttgc cggcccagta cacgaccgaa acggcaataa gatggaccag
      361 ccaccagccg aggttcggat accgccaggc gatacctttc ttctcgacgt cggcattata
      421 tcccatgacc tccagatcga acagcgcctt gcccacgaag aactttcccc agactatcag
      481 gatatcggga agggtcgtcc cgagaaaaat gccgccgatg ctcagcgggt caggcaggta
      541 gtggatcgag aaatggccca tggtgatcgt ggcaactatc gtgatgaaac cgttcagcac
      601 atacccgtaa tgcaccgtct tcctgatgga aaaaagaacc ggaacgacaa ggatgctcag
      661 gaggctggcc acgatgttga caagaatcga gctgtgctgg ctgaatggat gtacgcgaag
      721 atgaagcata aaaccaccga ggctcatcgt gacgagggcc gcgagaagga cagtcctcat
      781 gtaaaccttt accgtcatca cagctcctcc ccacggtctc catcacggcc caccgcgttc
      841 ctcacccgac tgttccctgt cgggttatcc agcatacaac caatagaagc attcccgacc
      901 ctcctgtcaa ccgggtcgat gtgggaaccc ttgtgcggca acctcctttt ccttgacata
      961 cctcttttgc ataagcgaca ttattggtct attccgatca gtttaacttc agaatgaggt
     1021 gagagcatga gctacttgtt gctggcggtc acattcataa tcagtctcct gttgccggcg
     1081 tcgtcgctcc acgcgggacc ggtggttgcc gtgaaggact ttcagggaag cgggccttcg
     1141 tcccagctgg gatcgggact ctcttccatg gtggagaccg aactcgcgaa ttcagaaggg
     1201 ctgaaaaaat gcggtggctc ggtggttgag tggaagagac gcaaggacgt ggttgatgag
     1261 atcgagcgtc agcagggacc cggatttgat ccgaagagtc gcgccaaaaa ggggaagctc
     1321 ctggagccga ctgtttttgt cgtaggggat gtcatcacca gtgacacaga cgtgcgctgg
     1381 tcgatcaagc tggttgaccc ggccacgggg cgcaccatcg ggcgtcacga cggaagtgtt
     1441 cccgcggaca aggacagcgt gttcgaggcg ccccgtgaaa tagcagagaa cctgggggag
     1501 cagatctgtc gtcaaggcat ggagtacacg tccccggcgg gcagatcggg caacgccggg
     1561 acatcggact ccccggcagg tgacccttcc ggggtgctgg acaaggtgaa tggggtagtg
     1621 gactcgatca agggattcaa aaaactgttc taggtgtgtg gtttgcggtc tgcctgctca
     1681 acgggaacaa ccggctggat cccccggtcc gttccgctct cctggcaggt tcggccgccc
     1741 ctgctcattg tttccgttac cctgcttgtc tatctcaact ccctgagcgg ggtcttccag
     1801 ttcgacgact ataatgtcat cgtcaacaac cccgcggtcc acggctggtc acagtggtgg
     1861 cgcggcgtgg ggggcattcg tccgctgctc aaactgagct acaccctgaa ctggacttcc
     1921 ggtttcgggc ttattggttt tcatctcttc aatgtcctcg tgcatgtcgt gaacgccctg
     1981 cttgtctacc gccttgcgca ggcggtcgga aggcgatcgt ttcgaccggg atcggctgaa
     2041 aaagcggcgt ttaccggcgc gcttctcttc gctatccacc cggtgcaaac ggaagcgata
     2101 acctacatca gcggccgctc cagttcactt gccgcctgct tctacctggc cagcctgctc
     2161 tgctacctgc agggccaccg aagggacggg cccacctggc cacaagctct ttccgccata
     2221 ttcttcatcc tggccctgct caccaaagag gcggccgtta c
//
LOCUS       DAOQTR010000386         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_59999_length_2251_cov_3.197177, whole genome shotgun sequence.
ACCESSION   DAOQTR010000386 DAOQTR010000000
VERSION     DAOQTR010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59999_length_2251_cov_3.197177"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PKN85_08955"
     CDS             <1..1186
                     /locus_tag="PKN85_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT44578.1"
                     /translation="KDMGLPWKLRIGIATGYTIVGMMGVHRQAYSAIGDRVNVAKRLE
                     EICEPERIYVDEYTFNFVEPFVNVKKLRNMGYSRQSDKALLEELRVLEHNLAEEGESA
                     DILYQMGKAHFGLHDASAAIACFERALALDPDSTDLRLAYADANLKKDEFEKFQLKGK
                     LHKIAVYEVRSIKSRWHDHTIIPRAVSERYGDVEAAMKVPEDLVLAVESLDGSIGRGL
                     LVGMLSYALADRLNLNEDLKLNIRQAGVLQDIGKEAVPHHILNRQASLTEQEIKFVEK
                     YVLESVASLKRLGYVNDQFLEIVKHHHELWNGNGYPDGLKGEEIPIGARITAVAEAYS
                     AMTSWRPYRGPWDVRLALNEIRKNTEKGFYDPQVVDVLFEILKFDASLVDRGEVSSLG
                     SK"
     gene            1383..>2251
                     /locus_tag="PKN85_08960"
     CDS             1383..>2251
                     /locus_tag="PKN85_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44579.1"
                     /translation="MRYPDGGRPVRKRPSPPCRGYRAFLWSLVVICAVLCLTFPAAGA
                     DLIPSYSEALEMAIRNASSPSGWQPASLYVIPDGGGTQGLFYDAGKLVVRTATKSRYY
                     GSNYVGQAGQKIFGGASTDAAWVTVGNDATRFLLTNGVTGSNVTTLLERGLGMDATGT
                     HDAIIEYAVDSQYLLRPARNPDISVYTPAKYGQSLPFEKPAGMSDDTFNNFKAYYTNW
                     LSQAYGSSRFPFTQLGYTFFWGNGTSPEDINGMTEFILLGQTPVDIYGVYATGSYIYT
                     RNDGTDFSTAATAS"
BASE COUNT          491 a          682 c          655 g          423 t
ORIGIN      
        1 gaaggatatg ggccttccct ggaagctgcg cataggcatc gccacgggat acacgatcgt
       61 cggcatgatg ggagtgcacc ggcaggcata cagtgccata ggcgaccgcg tcaacgtcgc
      121 gaagcgcctt gaggagatct gcgaacccga acggatatac gtggacgagt acactttcaa
      181 cttcgtcgaa cccttcgtca acgtgaagaa gctgcgcaac atggggtaca gccgtcagag
      241 cgacaaggcc ctcctcgagg aactcagggt cctcgaacac aatcttgccg aggagggaga
      301 gagcgctgac attctctacc agatgggaaa ggcacacttc ggcctccatg acgcctctgc
      361 cgccatcgcc tgttttgagc gcgcactcgc tctcgacccg gattccaccg atctcaggct
      421 tgcctacgcc gatgcaaacc tcaagaagga tgaattcgag aagttccagc tcaagggcaa
      481 gcttcacaag atcgcggtct acgaggtcag gagcataaaa agccgctggc acgaccacac
      541 catcatcccc cgggccgtat cggagcggta cggagatgtg gaggcggcaa tgaaggtgcc
      601 ggaggacctc gtccttgccg tcgagtctct ggacggttcc atcgggcgcg ggcttctcgt
      661 gggaatgctt tcctatgccc tggcggaccg tctcaatctc aatgaagacc tgaaactgaa
      721 catacggcag gccggagtcc tgcaggacat aggcaaagag gccgtacccc accatatcct
      781 gaaccgccag gccagtctga cggagcagga gatcaagttc gtggaaaaat acgttcttga
      841 aagcgtcgcc tccctgaaac ggctcggtta cgtcaacgac cagttcctgg agatcgtcaa
      901 gcaccatcac gagctgtgga acggcaatgg ctaccccgac gggctcaaag gtgaggagat
      961 acccatcggc gcccgcataa cggcagtcgc cgaggcatac agcgccatga catcctggcg
     1021 cccctatcgc ggcccctggg acgttcgtct tgcgttgaac gagatcagaa agaacacgga
     1081 gaagggtttt tatgatcccc aggtcgtcga tgtgctcttc gagatcctca agttcgatgc
     1141 ctccctggtg gacagaggcg aggtctcctc gctgggcagc aagtaggcgc caaagccggg
     1201 cggcacgaac ctcccatttc aagaacaaac acgccaacag accctgcccc gacctcccct
     1261 ggaactgcgc tatccggggt gtggacaatc gtcacaaaac cttgtagtat ctttgcggcg
     1321 cccggcgagg acatctctca ttcccctgcg cgcgcacatc attggtcggg aggcgaaggg
     1381 ctatgagata cccggatggt gggagaccgg tgcgaaagcg cccttccccg ccgtgtcgag
     1441 ggtaccgtgc attcctgtgg tccctggtcg ttatttgtgc tgtcctttgt cttacctttc
     1501 ctgccgccgg ggcggacctc atcccgagct acagcgaggc cctcgagatg gcgatacgga
     1561 acgcctccag tccgtcgggc tggcaacccg cgtcgctcta tgtcataccc gatgggggag
     1621 gcacccaggg gctcttctac gatgcgggga agcttgtcgt tcggacggcc acgaaatccc
     1681 gctattacgg aagcaattac gtcggtcagg cgggccagaa gatctttgga ggggcctcga
     1741 cggatgccgc gtgggtgacg gtcggcaacg atgccaccag gttccttctg accaatgggg
     1801 tgaccggaag caatgtgacc accctccttg aacggggact gggcatggac gcgacaggca
     1861 cgcacgacgc gatcatcgag tatgcggtgg attcccagta cctcctgcgt ccggcgcgaa
     1921 accccgacat cagcgtgtac acgcccgcca agtatggtca gagccttcct ttcgagaagc
     1981 cggctgggat gagcgacgac acgttcaaca actttaaggc ctattacacc aactggttgt
     2041 cgcaggccta cggttcgtcc cgcttcccat tcacacagct ggggtacacc ttcttctggg
     2101 ggaacggcac atctcccgag gacattaacg gcatgacgga gttcatcctc ctcggtcaaa
     2161 cccctgtgga catctatggc gtgtacgcca ccggttcata catctacacg cgaaatgacg
     2221 ggaccgattt cagcacggcg gcaactgcct c
//
LOCUS       DAOQTR010000387         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_60073_length_2249_cov_3.370100, whole genome shotgun sequence.
ACCESSION   DAOQTR010000387 DAOQTR010000000
VERSION     DAOQTR010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60073_length_2249_cov_3.370100"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="PKN85_08965"
     CDS             complement(<1..1396)
                     /locus_tag="PKN85_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNT44580.1"
                     /translation="MKRSIAAAAFIILLLAGCATNNQVSMGGIGSQGYPVVVQDQAPA
                     GTVDAEGDSSRVSGMGKGTAARSMRTKAINAHTRQQQSVSRKAKPQPYSLEVKVDDDD
                     DITTLLTTNYDQNFDIPIVFNDAVKYYIKWFSEDKKKVFANWLKRSRLYVPIITEILR
                     EKNMPEDLVYLAMIESGFNPKAYSHAKASGPWQFIYSTGGRYGLEVDHWVDERRDPEK
                     STVAAAKYLKDLFDQFGCWYLAAAGYNAGEGRIGRLIQKHNTNDFWELYKYNTLPRET
                     RNYIPQLIAAAIIAKDPAKFGFGSIVYDAPVRFTEVGVPAATPLAAIARASNCDMETI
                     RTCNPEVLRGITPPGKSDYMVKLPSDVDKGAFAERLATELDKLPSVKSVVTYKVRKRD
                     SMARIVKKYKVSERDLVLVNSSDDDLKIKPGMVIAIPKFSGETARSTVLARDVTPDRS
                     KARRKSDDDSPARVL"
     gene            complement(1490..>2249)
                     /gene="fmt"
                     /locus_tag="PKN85_08970"
     CDS             complement(1490..>2249)
                     /gene="fmt"
                     /locus_tag="PKN85_08970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNT44581.1"
                     /translation="LVEIGSFRDDEARSIADFHPDLYVVVSFGLIVPKWALDIPSIAP
                     VNIHPSLLPLYRGPSPMQWALLGGDAVTGITFIKMNEKMDEGDIIGQEEVAIEKDDDY
                     TTLSKRLSEKAAQMLQKVVDDIGKHGMMEGTRQDHSAATYAPIITKEMGRIDWTRGAA
                     RVDCQVKAFVEWPTAFTYLDGRLLKIYSSFADESVQAQAAAGTVTSITKAGLEVATGK
                     GLLVVRELQLENKKRMKAYDFSIGYRDLKGKLLN"
BASE COUNT          443 a          653 c          590 g          563 t
ORIGIN      
        1 acaggaccct ggcgggactg tcgtcgtcgc tcttccgcct ggccttagac cgatccgggg
       61 tcacatccct ggcaagcaca gtgctgcgtg ccgtctctcc tgagaacttc ggtatggcga
      121 tgaccatacc cggcttgatc ttgaggtcgt catcgctgct gttcacaagg acgagatcac
      181 gctcgctgac cttgtacttc tttactatcc ttgccatgct gtcccttttc cgaaccttgt
      241 acgtgacgac gctcttcacg gacgggagtt tatcaagctc ggttgcaaga cgttcggcaa
      301 aggccccctt gtcgacgtcc gacggaagtt tcaccatgta atcgctcttt cccggagggg
      361 tgatcccccg gagaacctcg ggattgcatg tgcgaatggt ctccatatcg cagttcgagg
      421 ccctggctat tgctgcgagc ggggtggccg cggggacccc gacctctgtg aacctgacag
      481 gagcgtcata gacgatactg ccgaagccga atttcgcggg gtcttttgcg atgatggccg
      541 ccgctatcaa ctgggggata tagttccgtg tctcacgggg aagggtgttg tacttgtaca
      601 gctcccagaa atcgttcgtg ttgtgcttct ggataagccg ccctatcctc ccctctcccg
      661 cattgtagcc ggcagcggca agataccagc aaccgaactg gtcgaaaagg tccttgaggt
      721 acttggcggc ggcaacggtt gatttttccg gatccctgcg ttcgtccacc cagtggtcaa
      781 cctcaaggcc ataccgccca cccgtggaat atatgaactg ccacggccct gatgcctttg
      841 catgcgaata agccttcggg ttaaaaccgc tctctatcat ggccaggtag acaaggtcct
      901 cgggcatgtt cttctcccgg aggatctcgg tgatgatggg aacgtacagg cgggaacgct
      961 tcagccagtt ggcgaacacc ttcttcttgt cctcggagaa ccatttgatg tagtacttca
     1021 ccgcgtcatt gaacacgatg gggatgtcaa aattttgatc gtagttcgtg gtgaggaggg
     1081 tcgttatgtc gtcgtcgtcg tcgaccttca cttccagcga ataaggttga ggtttggctt
     1141 tccgcgagac gctctgttgt tgtctcgtat gggcattgat cgctttcgtt ctcatcgacc
     1201 gtgcggcggt acccttcccc attccggaaa cccgggagga atccccctcc gcgtccacag
     1261 tcccggcggg ggcctggtcc tgcacgacga cgggatatcc ctggctgccg atgcctccca
     1321 tcgatacctg attgttcgtt gcgcatcctg cgagaagaag tataataaat gctgctgcgg
     1381 cgatagatcg tttcatggtc cccttaccgt agcctctttc taatggcctt atcttgatga
     1441 tatgtgcaat atgcctttct tgtcaataaa aaaatcgaca ttcatgaagc taattcagca
     1501 acttaccttt taagtccctg taacctatgg agaaatcgta tgccttcatg cgcttcttgt
     1561 tctccagctg aagctccctg acaacaagca aacctttgcc ggtcgccact tccaggcccg
     1621 ccttggttat gctcgtcacg gtaccggcag ctgcttgtgc ctgaacgctc tcatcggcga
     1681 acgagctgta tatcttgagc agtctcccat cgagataggt aaaagcggtg ggccactcta
     1741 caaaggcctt cacctgacag tcaacacggg cggcgcccct ggtccagtct atccgtccca
     1801 tctccttcgt gatgatgggg gcatacgtgg ccgccgaatg atcctgtctc gttccttcca
     1861 tcataccgtg cttcccgata tcatccacca ctttctgcaa catttgtgcc gctttttccg
     1921 aaagcctctt agacagggtg gtatagtcgt catccttctc tatcgccact tcttcctggc
     1981 cgatgatatc cccctcgtcc atcttctcgt tcatctttat aaacgtgatc cccgtgaccg
     2041 cgtcgccgcc caggagagcc cattgcatcg gcgagggacc tcggtatagc ggcaaaaggg
     2101 aaggatgaat attgacggga gcgatggacg gaatgtcgag ggcccacttc ggcacgataa
     2161 gcccgaagga caccacaaca tagaggtcgg ggtgaaagtc ggctatggac cgcgcctcgt
     2221 catccctgaa ggagcctatc tcgacgagg
//
LOCUS       DAOQTR010000388         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_60379_length_2242_cov_3.203018, whole genome shotgun sequence.
ACCESSION   DAOQTR010000388 DAOQTR010000000
VERSION     DAOQTR010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60379_length_2242_cov_3.203018"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PKN85_08975"
     CDS             complement(<1..979)
                     /locus_tag="PKN85_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44582.1"
                     /translation="MSPGTSPAGHPFAIDPGTFLGFCEKWCAILGGGAPGEGDIRHLI
                     SALRIAAGLALFLLFWVWILIRSSRRRKKQLKGVNERYRLLVENTSEGIMVIVEGKAV
                     FMNERASQITGFSLHELTPRALSEITGSQDGNAVKQAHRRIMRSEVKSLRLPIRITCK
                     NGETKWIHNCWVGVTWKGTPGVLCVFSDITESKKMEQQFLQAQKMEAIGQLAGGIAHD
                     FNNILTAISGYGNLLRIKTDNDPALNRYARNILACSNRAANLVKGLLAFSRKQVNDPR
                     PTDLHDVIKGAEKMLRRIIGEHIEFSVIFSKTTWRSVGRGSLTCFLLKAR"
     gene            1364..1870
                     /locus_tag="PKN85_08980"
     CDS             1364..1870
                     /locus_tag="PKN85_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44583.1"
                     /translation="MKIMTGIVHVVCILVVLCVLLTLPGCTGEGEKGSGPQAAKTEPQ
                     ARQAKAGGFTVPEEGTPAERVRKLWESTGPIMKVDAQANLIDREYQNKRTPLFSVWVT
                     LQGKLSLAPQKDERAQRVIPEVLKFIDDLYGFPGFTEERREKDRETAKKFFDKRYGRL
                     EEEIKALK"
     gene            complement(2048..>2242)
                     /locus_tag="PKN85_08985"
     CDS             complement(2048..>2242)
                     /locus_tag="PKN85_08985"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNT44584.1"
                     /translation="NGVVAFRGSRFVAPPKNLVGAGGREHITFSATAQGTQKLTFHYL
                     RPWERNTEPAKTIVFTVLVR"
BASE COUNT          482 a          603 c          675 g          482 t
ORIGIN      
        1 ttctcgcctt cagcaggaag caggtcaacg acccgcgtcc gacagacctc cacgttgtct
       61 tcgaaaagat gacggagaac tcgatgtgct ctccgatgat gcggcgaagc atcttctccg
      121 cgccctttat gacatcgtgg aggtctgtcg gacgcgggtc gttcacctgc ttcctgctga
      181 aggcgagaag gcccttcacg agattcgcgg cgcgattgga acaggcgagg atgttccggg
      241 cgtacctgtt gagggcgggg tcgttatcgg tcttgatcct gagcaggttt ccgtacccgc
      301 tgatcgcggt gaggatgttg ttgaagtcgt gggcgatccc tcccgcgagc tgcccgatgg
      361 cctccatctt ctgggcctgg aggaattgct gctccatctt cttgctctcc gttatgtccg
      421 aaaaaacgca taggacgccg ggggtgcctt tccaggtgac gccgacccag caattgtgta
      481 tccatttcgt ttcgccgttc ttgcaggtta tccggatggg aagcctgaga ctcttgacct
      541 ccgacctcat gatcctgcga tgggcttgtt ttacggcatt accgtcctgg gaacccgtga
      601 tctcggagag ggcacggggg gtaagctcat ggagggagaa gccggtgatc tgcgaggccc
      661 tctcgttcat gaaaacggcc ttcccctcaa cgatgaccat aatgccctcg gacgtattct
      721 ccacaaggag gcggtagcgt tcgttcaccc ctttcaattg cttcttgcgc cgtctcgagg
      781 acctgataag tatccagacc cagaagagaa ggaagagcgc gaggccggca gcaatgcgta
      841 gggccgagat gaggtgccgg atatctcctt cgccgggggc gccgcctccc aggatcgcgc
      901 accatttctc gcagaatccc aagaatgttc caggatcgat ggcgaaggga tgccccgcgg
      961 gggaggtccc cggcgacatg aggccgcctg atccgcctgt gtatgatgac gccccgcccg
     1021 cgacggggga caggaccgca acaagggccg ctaccatgag aacagcggcg aaatgtctat
     1081 atagagcacg acagcgcatt ttccggtcag ttatgaaaac cgttcaatga tggaaacctc
     1141 tcaactgcct gatgactgct tgcgctaaaa aagtaaaagt acatgttatt gtttgtctta
     1201 tttgctctat cttataccag cagggaggat catggcaaca acaaaaacac ggatcgccgg
     1261 agtgcatgcg ccagcggagg gtgacggtgt attgcaatgt tctcgatcgg atgctatatt
     1321 ccgtacttaa cgcgtgagca caacatcctc tcagggaggc gccatgaaga taatgaccgg
     1381 cattgtccat gttgtctgta tccttgtcgt tctctgcgtg cttctcaccc tgcccggctg
     1441 caccggagag ggggagaaag gctcggggcc gcaggccgcg aagaccgagc cgcaggcccg
     1501 ccaggcgaag gcgggaggct tcaccgttcc cgaggaaggg acgccggctg aaagggtcag
     1561 gaaactctgg gaatctacgg gtcccatcat gaaggtggac gcgcaggcga accttatcga
     1621 cagggagtac cagaacaaga ggacgcccct cttttcggtc tgggtcacgc tgcaggggaa
     1681 actctccctg gcccctcaga aagatgagag ggcgcagcgg gttatcccgg aggtcctcaa
     1741 gttcatagac gatctctatg gtttcccggg attcaccgag gaacgccgcg aaaaggaccg
     1801 cgaaacagca aagaagttct tcgacaagcg atacggcagg ctggaggaag agataaaagc
     1861 attgaaatga gctgcttgaa ccgcttgaac agcttaagct gcttgaacgt tgaaaacaaa
     1921 agaccatagg gaaggagtac ggcctgactc ccggttgtga tccggggggc gaattgtgtc
     1981 tgtctctttg tttttaggga ccgcaggggt tcttgcgctc aagcggctta agctgttcaa
     2041 gcggtcttca ccgtaccagt acggtgaaga caattgtttt tgccggttcg gtgttccttt
     2101 cccaggggcg gaggtagtga aaggtcaact tttgcgttcc ctgtgcggtg gcactgaagg
     2161 ttatgtgctc cctgccgccg gcaccaacga ggttcttcgg aggggcgaca aacctgctgc
     2221 ctctgaaggc aaccacaccg tt
//
LOCUS       DAOQTR010000389         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_60575_length_2237_cov_3.986709, whole genome shotgun sequence.
ACCESSION   DAOQTR010000389 DAOQTR010000000
VERSION     DAOQTR010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60575_length_2237_cov_3.986709"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1985)
                     /gene="htpG"
                     /locus_tag="PKN85_08990"
     CDS             complement(78..1985)
                     /gene="htpG"
                     /locus_tag="PKN85_08990"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNT44585.1"
                     /translation="MTSERMEFRTEVKQLLDLMIHSLYSHKEVFLRELVSNASDAIDR
                     ARYESLTNSDILEEGSGWKIKLFVDRDAGTLTVSDNGIGMTHDEIIESLGTIARSGTK
                     EFLEALKGKEVKDNPELIGQFGVGFYSSFMVADRITVISRRAGSKETKGVKWESAGDG
                     TFTVDEVEKESRGTDVILHLREDEKAYLSEWEIRNVVKKYSDFIEHPVVMDVEKEVES
                     ELQKGEKVKVRQEETLNSMKALWLKDRSEVASEEYDDFYKHISHDFHPPAKVIHYRAE
                     GTSEFAALMFVPSVAPVNMLYRDFKYGPMLYVKRVRIMDHCEDLIPQYLRFLRGVVDS
                     SDLPLNVSREILQNNRQVDIIKNSITKKILDTFEEMKESSYDDYVKIYGQFGRVLKEG
                     VHFDFSRREAIADLLLFASTRSGEGKFRTLKEYVDDMKEGQEEIYYIVGSSLSDAANS
                     PYLEAFKEKGFEVIYMTDDIDDFIFNGFEYKGKKMHSITRGDISLDKDKTVQIEETKK
                     RYARLIDLIKETLSGEVKDVRLSGRLTDTVCCLVADEGDLDPAMERLLKAMGQDLPPM
                     KRILELNPSHPLFAAMNAIFEKGEEGTELKDYVRLLYDQALVLEGSKPKDPAAFSKAI
                     AKLMVENAEKK"
BASE COUNT          436 a          623 c          609 g          569 t
ORIGIN      
        1 ggtcattggt aataggtcat gggctgccga ctgtctgttc ttcccataac ctataaccca
       61 taacctataa cctgtcttca tttcttctcc gcattctcga ccatcagttt tgctatggcc
      121 tttgaaaaag ccgccgggtc tttcggtttc gatccctcca gcaccaacgc ctggtcatag
      181 aggagccgga cataatcctt cagttccgtg ccttcctccc ctttctcgaa gatggcgttc
      241 atcgcggcga agagcgggtg ggaggggttg agctccagta tcctcttcat aggaggcagg
      301 tcctggccca tggccttgag aaggcgttcc atggccggat cgaggtcgcc ttcatcggct
      361 acgaggcagc acaccgtatc ggtaagcctt cccgagagcc tcacatcctt gacctccccg
      421 gagagggttt ccttgatgag atcgatgagc cttgcgtacc tcttcttcgt ttcctctatc
      481 tggaccgtct tgtccttgtc gagggagata tcgcccctcg ttatggagtg catcttcttt
      541 cccttgtatt cgaagccgtt gaaaatgaag tcgtctatgt catcggtcat gtagatgacc
      601 tcgaacccct tctctttgaa ggcctcgagg tagggcgagt tggcggcgtc cgacagggat
      661 gagccgacga tgtagtatat ctcttcctgg ccttccttca tgtcgtccac gtattccttc
      721 agggtccgga acttgccttc cccggaacgc gtggaggcga agaggaggag gtcggcgatg
      781 gcctctctgc gggaaaagtc gaaatggacc ccttccttga ggacgcggcc gaactgaccg
      841 tagatcttca cgtaatcgtc gtagctgctc tccttcatct cttcgaaggt gtcgaggatc
      901 ttcttcgtaa tggagttctt tatgatatcg acctgccggt tgttctggag gatctcgcgg
      961 gagacattga ggggcaggtc tgacgaatcg acgacgccgc gcaggaaccg cagatactgg
     1021 ggtatgaggt cctcgcaatg gtccatgata cggacccttt tcacgtagag catgggcccg
     1081 tacttgaaat cgcggtagag catgttcacg ggagcgacgg agggtacgaa catgagggcg
     1141 gcgaattccg atgtcccttc cgccctgtag tggatgacct ttgcgggggg atggaagtcg
     1201 tgggagatgt gcttgtagaa gtcgtcgtat tcctcgcttg cgacctccga cctatccttg
     1261 agccagagcg ccttcatgga gttgagggtt tcctcttggc ggaccttgac cttttcgccc
     1321 ttctgcagct cgctctccac ttccttttcc acatccatca cgacggggtg ttcgatgaaa
     1381 tcggagtact tcttcaccac attccggatc tcccattcgc tgagataggc cttctcgtcc
     1441 tcgcgcaggt ggaggatgac atcggtgccg cgcgactcct tttcgacctc atcaacggtg
     1501 aaggtcccgt cgcccgcgga ttcccacttt accccttttg tttccttcga gccggcgcgc
     1561 cgtgagatga ccgttatcct gtcggcgacc atgaaggagg agtaaaagcc cacgccgaac
     1621 tggccgatca gctccgggtt gtccttcacc tccttgccct tcagggcctc gaggaactcc
     1681 ttcgtgcccg agcgggcgat ggtgcccagg gattctatga tctcgtcgtg ggtcattccg
     1741 atgccgttgt cactcaccgt gagcgtcccg gcatccctgt cgacgaagag ctttatcttc
     1801 catccgctgc cttcctccag gatgtcactg ttcgtcagcg attcataccg cgccctgtcg
     1861 atggcatcgg aggcgttgga taccagttcc ctgaggaaaa cctccttgtg tgagtagaga
     1921 gagtggatca taaggtcgag aagctgcttg acctccgtcc tgaattccat ccgttcgctt
     1981 gtcataaccc ggtcacctca aattagattg ttttttcgca caaaaactaa gacagggagg
     2041 ggaaggagtc aataggggtt aaagaaggtt caagggttca agggttcaaa agttcaagaa
     2101 taaaagacaa aaagggcacc ggatgccttc ggccggtgtg cggattgcgt tgacagccga
     2161 tctgcacgtg tcactaaaag cgtgtcaagg accaggttaa ttccccacta ccggggccaa
     2221 ggtaattccc cactttt
//
LOCUS       DAOQTR010000390         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_61327_length_2220_cov_3.024942, whole genome shotgun sequence.
ACCESSION   DAOQTR010000390 DAOQTR010000000
VERSION     DAOQTR010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61327_length_2220_cov_3.024942"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1303)
                     /locus_tag="PKN85_08995"
     CDS             complement(176..1303)
                     /locus_tag="PKN85_08995"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44586.1"
                     /translation="MERYLYEPIKKDLTRKMVILTGPRQIGKTWLARELMKEFKTPQY
                     LNYDSFDDAKIIQAHTWPIHADMLVFDEIHKMKGWKKFVKGTFDTRPTRQAILVTGSA
                     RLDTFRQAGESLAGRYFHFRLNPVSVRELKGAEKPFEALTLLNRLGGFPEPFLSGSDE
                     EAARWRNQYYTDLVREDILEFSRIQEVRAIRLLLEMLRERVGSPLSYTSLARDLQVAP
                     NTVRKYVEILENLCIIFLVRPFHTNVARAVLREPKIYFYDSGYVRGDEGIKLENTCAV
                     CLLKHVHYLQDTAGEEITLHYIRTRDGREIDFAIARKGKVDQLIEVKLSDESLSAGLR
                     YFLTMFPGVPAFQLVHNLRREQSVAGVDIVHASKWLSELSA"
     gene            complement(1590..>2220)
                     /locus_tag="PKN85_09000"
     CDS             complement(1590..>2220)
                     /locus_tag="PKN85_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44587.1"
                     /translation="REEINGIARRVGLHTILNTVLNRSGEVIEAFFGDVVEAFNEDVR
                     LSKQVYAAEIPEEADIVLAGSHPCDIEFWQAHKTLYPSDLAVKEGGIVIVVTPCPEGV
                     AMTHRDILEVACHDARGLRGMVSCGQLHDEVAAALAIAWAQVKERETVYLVSDGIGAE
                     DAEKLGFTHFTNVQDALDAALRLKGDGARVTVLTHAPDMLPVIVPNEGK"
BASE COUNT          416 a          647 c          683 g          474 t
ORIGIN      
        1 gacggtgtga attctctacc cccatagccg cttcgctgcc gcgaccagtg gtcggtgggg
       61 ccaacggccg cagactacag gcttcaggca tcgggattcg ggaagaaggg tttcagcggt
      121 tcaggggcgc agggaaatgc cgcagggcgg catggcttcg ggactcggga ttcgctcagg
      181 cgcttagctc agaaagccac ttggatgcat gaacgatgtc cacgccggcg acgctctgct
      241 cccgcctcag attgtgaacg agttgaaatg ccgggacccc gggaaacatg gtgaggaaat
      301 atcgcaggcc ggctgagagg ctttcatcgg agagtttcac ctcgatgagc tggtcgacct
      361 ttccctttct tgcgatggca aaatcgattt cgcggccatc gcgcgtccgg atgtagtgca
      421 gggtgatctc ctcccctgct gtgtcctgca gataatggac atgcttcaaa agacacaccg
      481 cgcaggtgtt ttcaagttta atgccttcgt ccccgcgaac atagccgctg tcgtaaaaat
      541 agatcttcgg ctctctcaaa acggcccggg cgacgttggt gtgaaaaggg cggaccagga
      601 agatgatgca gaggttctcc aaaatctcaa cgtactttcg aacggtattg ggcgcaacct
      661 ggaggtcgcg cgccagtgag gtataggaca ggggggagcc gacccgctcc ctcagcattt
      721 ccagcaggag ccggatggcc ctcacttcct gtattcggct gaattccaga atgtcttccc
      781 gtacgaggtc cgtgtaatac tggtttctcc agcgtgcagc ctcttcatcc gatccggaaa
      841 gaaacggctc agggaaccct ccgaggcgat tgaggagggt aagggcttcg aaaggtttct
      901 ccgcaccctt gagttccctg accgagacag ggttcaggcg gaagtggaaa taacggccgg
      961 ccaatgattc ccctgcctgg cgaaaggtat cgagcctcgc acttccggtc accaggatgg
     1021 cctgtcgcgt cggtcgagtg tcaaacgtac ccttgacgaa cttcttccac cctttcatct
     1081 tgtggatctc atcaaagaca agcatatccg catggatcgg ccaggtgtgc gcctggatta
     1141 ttttcgcatc gtcaaaactg tcataattca ggtactgggg ggtcttgaac tccttcatca
     1201 gttcccttgc cagccatgtc tttccgatct ggcgtggccc ggtcaggatg accattttcc
     1261 gggtaagatc cttcttgatc ggttcgtaaa gatagcgttc catgtgcgac atttacgcaa
     1321 gataatgcac aaaagtcaag acttttgtgc agcatggtgc atgttcgcca atgttgccgc
     1381 aggcagcttc gctgccgtga cgggttgaca aggagaacag gtccacaggt tcaggggtgc
     1441 agggaaggca gtctgtccgg tctgtccggt ccgtccggct gttccgtcta agaagatcta
     1501 ccgggtctat tcgttcattt cgtttgtttc gttgggggaa gttcctggaa cgccgcgcct
     1561 cgcaaggccc gtctcgcgat gatccctcct cacttcccct cgttcggcac gatcaccggc
     1621 agcatgtcgg gcgcgtgggt gaggacggtg acgcgggccc catcgccctt cagccgcagg
     1681 gccgcgtcga gggcgtcctg gacgtttgtg aaatgagtga accccagctt ctcggcgtcc
     1741 tcggccccga tgccgtcgga gacgaggtag accgtctcgc gctccttcac ctgggcccag
     1801 gcgatggcca gggccgcagc gacctcgtcg tgcagctgcc cgcaggagac catcccgcgc
     1861 aggccgcggg cgtcgtggca cgcgacctcc aggatgtccc ggtgcgtcat ggccaccccc
     1921 tccgggcagg gggtcacgac gatgacgatc ccgccctcct tgacggccag gtcggagggg
     1981 tagagggtct tgtgcgcctg ccagaactcg atgtcgcagg ggtgggagcc ggccaggacg
     2041 atgtcggcct cctcggggat ctccgcggcg tagacctgct tcgagagcct gacgtcctcg
     2101 ttgaaggcct ccactacgtc gccgaagaag gcctcgatga cctcgccgct gcggttgagc
     2161 accgtgttga ggatggtgtg gaggcccacc cggcgggcga tgccgttgat ctcctcgcgg
//
LOCUS       DAOQTR010000391         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_61415_length_2218_cov_2.779935, whole genome shotgun sequence.
ACCESSION   DAOQTR010000391 DAOQTR010000000
VERSION     DAOQTR010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61415_length_2218_cov_2.779935"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKN85_09005"
     CDS             <1..509
                     /locus_tag="PKN85_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HNT44588.1"
                     /translation="QVGALAGFTSRAGVSLQHVKLHGALYNRVSGEENVFLQIVDAVR
                     KAFGDLVFITLASPASAELKERCRSLGVRVALEAFPDRAYTDDGTLMPRGRDGAVMSD
                     AEEIARRAVAMVRDGCVESVNGRRIELEIDTLCVHGDNRESIAASPLIREHAAREGMA
                     VKPLYSFV"
     gene            513..1154
                     /gene="pxpB"
                     /locus_tag="PKN85_09010"
     CDS             513..1154
                     /gene="pxpB"
                     /locus_tag="PKN85_09010"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNT44589.1"
                     /translation="MDIERFGEQGIRIIFGQAIDDATHESVRRFFLYTSSLRIPGITE
                     IIPSFRSCLISYDRERVGFEDLSELLGEMEDDWREMSLPDVRRMEIPVRYGGDYGPDM
                     DFVCSYSGLSPEDVVEIHAGATYRVYALGFMPGFPYLGPLDRRIYVPRQETPVLKVPQ
                     GSVGIAQLQTGIYPFESPAGWRVIGRTMVRLFDHLNPPFSLLNFGDIVRFVRA"
     gene            1151..2098
                     /locus_tag="PKN85_09015"
     CDS             1151..2098
                     /locus_tag="PKN85_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNT44590.1"
                     /translation="MIEVLSPGFLSIIVDRGRQGHGHIGVPSSRELDRHASAIARYLV
                     DIPEDAALLEVMGNDLRLRVLRDMSFAVTGAKVKATLDDEPLRPWAAVRAKEGSILRV
                     AEVLEGLRYYIAFSGIIDIEPVMGSSTTNLECNFGGFHGRPLMKGDMVALRDIREPAG
                     LQTVPETMIPSMREPHLVRVIAGPEADNFVPRSVRFLANRKETVLFNATSRLNRTGIR
                     LEGEPLRFRDDVERSIISEGLLPGTIQVPPDGYPIITLAERTIGGYARLATVADADMD
                     MLAQIRPRDRVVLSSIDINEAERLWRAGRDALSFYCKKQ"
BASE COUNT          403 a          650 c          707 g          458 t
ORIGIN      
        1 accaggtcgg ggcactcgcg ggattcacct cacgggcggg cgtatccctt cagcatgtga
       61 agcttcatgg cgccctgtac aatcgcgtgt caggggagga gaacgtcttc ctgcagattg
      121 ttgatgcggt gaggaaggcc ttcggtgatc tcgttttcat tacccttgcc tccccggctt
      181 cggcggaact gaaggagcgc tgccggtccc tgggtgttcg ggtcgccctt gaagcgtttc
      241 ctgacagggc ctacacggac gatggtacgc tcatgccgcg gggtcgggac ggtgccgtca
      301 tgagtgatgc cgaagagatc gcccgccggg cggtggcaat ggtgcgggac gggtgtgtgg
      361 agagcgttaa cggacgacgg atcgagcttg agatcgatac gctctgcgtc cacggtgaca
      421 accgggaaag catcgccgcg tctcctttga tccgggaaca tgcggcccgg gaggggatgg
      481 cggtcaagcc cctttactct ttcgtgtgat ccatggatat cgagcgtttc ggtgaacagg
      541 gcatcaggat catcttcggg caggcgatcg acgacgcgac gcacgaatcg gtgcggcgtt
      601 tcttccttta cacaagctcg ctccgcatcc ccggcatcac cgagatcatc ccctcgttca
      661 ggtcctgcct catcagctac gacagggaac gtgtcggctt cgaggacctg tcggaactgc
      721 tcggggagat ggaggatgac tggcgagaga tgtctcttcc cgatgtgcgg cggatggaga
      781 taccggtcag gtacggcggc gattacggac cggacatgga ttttgtctgc tcctattcgg
      841 ggctttcccc cgaggatgtc gtggagatcc acgcgggggc tacgtaccgg gtctacgccc
      901 tggggttcat gcccggtttt ccctatctgg gcccgctcga cagaaggatc tatgttcctc
      961 ggcaggagac ccccgttctc aaggtccccc agggttcggt gggcatagcc cagcttcaga
     1021 ccgggatcta tcccttcgaa tcgccggcgg gctggcgggt catcggcagg accatggtaa
     1081 ggctcttcga tcatctgaat ccgcctttca gtctcctcaa cttcggtgat atagtgaggt
     1141 tcgtacgggc atgatagagg tcctcagtcc cggttttcta tccatcattg tggaccgcgg
     1201 ccgacagggc cacgggcaca taggtgtccc gtcctcccgg gagctcgatc gccacgcctc
     1261 ggcgattgcg cgctatctcg tggacatccc cgaggacgca gcccttctcg aggtcatggg
     1321 aaacgatctc aggttgcgcg ttctgcggga catgagcttt gccgtcacgg gagcgaaggt
     1381 gaaagccacc ctcgatgacg agcccctgcg tccctgggcc gccgttcgcg cgaaagaggg
     1441 cagcattctc cgcgtcgcgg aggtcttgga ggggttgcgg tactacatag cgttttcggg
     1501 catcatcgac atcgagcccg taatgggcag ctcaacgaca aaccttgaat gcaacttcgg
     1561 cggttttcac ggcaggccgc ttatgaaggg agacatggtg gcattgcggg atatccgtga
     1621 gcccgcagga ctgcagaccg ttcctgaaac gatgataccc tcaatgcgcg agccccacct
     1681 ggtcagggtt attgccggac ctgaggccga caatttcgtc cccaggtcgg tcaggttcct
     1741 ggcgaaccgg aaggaaacgg tccttttcaa cgccacctca cggctcaacc ggacgggcat
     1801 ccggctcgaa ggggagccgc tcaggttccg cgacgatgtg gaacgaagca taatttccga
     1861 gggcctcctg ccggggacca tccaggtccc gcccgacggg tatcccatca tcaccctcgc
     1921 cgagaggacc atcggcggct acgcccgact ggccacggtg gcggacgccg acatggacat
     1981 gctggcccag ataagaccga gggacagggt agtcctgagt tccatcgaca tcaacgaggc
     2041 cgagcgcctc tggagggcgg gaagggacgc gctttccttt tactgcaaga aacagtgagg
     2101 caggggaggc agagtctcaa tgggcagggg gcagagcggg gtgaggggtg gatagggcct
     2161 attctcctat tcgtcctatc ctctgccccc ccctttccct ttctccttaa cctttttt
//
LOCUS       DAOQTR010000392         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_61808_length_2209_cov_3.233055, whole genome shotgun sequence.
ACCESSION   DAOQTR010000392 DAOQTR010000000
VERSION     DAOQTR010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61808_length_2209_cov_3.233055"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="PKN85_09020"
     CDS             complement(<1..1497)
                     /locus_tag="PKN85_09020"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNT44591.1"
                     /translation="MGEYGAESIKILGGLDAVRKVPSMYIGNTNIDGLHHLIYELVDN
                     SVDEALEGYCNRIAVTIHRDNSVTVEDNGRGIPVTMHSEENMSALEVVLTKLHAGGKF
                     DKDTYRYSAGLHGVGLSVVNALSHYLEVEVKRAGQVYFQRYEQGNRMTDLTVIGDTES
                     TGTKVKFKADATIFETTEYSYDTLAHRMREISFLNNGIHIILNDERKARKQDFKHEGG
                     IRSFVKYLNASKTVLFDEPIYVSNSRESLDLLEVALQYNDGYNENIQSFVNNVNTKEG
                     GTHVAGFRSALTRCVNNFIQSNMQGKVKENLTGDDIKEGLVAVVNIKVQNPQFEGQTK
                     AKLGNSEIKGIVESLLNEKIGEYLELNPQIARTIVGKALEARRAREAAKKAKELVKSK
                     SLIENGVLPGKLADCQESDPVQCELFIVEGDSAGGSAKQGRNRKTQAILPLRGKILNV
                     EKSRQEKVLTNQEIRSIYLAVGIGAEGKGRLRYHKVIIMTDADVDGSHI"
     gene            complement(1501..>2209)
                     /gene="dnaN"
                     /locus_tag="PKN85_09025"
     CDS             complement(1501..>2209)
                     /gene="dnaN"
                     /locus_tag="PKN85_09025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNT44592.1"
                     /translation="TIPGSILLEMIDKVEFAVSIDETRYILTGMFMKGQDDKVVVVGT
                     DGFRMALYQKEIDGLKPFQGMTIPKRSISEVERVIGEDEDVRVVIDEKHVQFSTEKMK
                     IISRIIEGNFPDYENVLPSGNTNVAEVERELLFKGLKKVSSIIGRSEPVKMTLSENTM
                     TIEAESDIGRAKEVIGITYDGEETTMNFNVKFLIDVVTHLTGDQVTIAAPKAYGAVLF
                     RSGDLDGYKNIVMPIRI"
BASE COUNT          384 a          655 c          555 g          615 t
ORIGIN      
        1 tatatgggaa ccgtcgacgt cggcgtcggt catgatgatc accttgtggt agcgcagccg
       61 tccttttccc tcggccccaa tgccgacggc gagatagatg gagcgtatct cctgattcgt
      121 caacaccttt tcctgccgcg atttctcgac gttgagtatc ttccctctca agggtagtat
      181 ggcctgtgtc ttcctgttcc tgccctgttt cgccgaaccg cctgctgaat cgccctcgac
      241 aatgaaaagc tcgcactgca ccgggtcgct ttcctggcag tccgcaagtt ttcccggcag
      301 caccccgttc tctataaggc tcttgctctt gacgagctcc ttcgcctttt ttgccgcttc
      361 ccgggcccgg cgcgcctcga gggccttgcc gactatcgtc ctggctatct ggggattgag
      421 ttccaggtat tcccctattt tctcgttcag gagggattcg acgatgccct ttatctcgct
      481 gttgccgagc ttcgccttcg tctgcccctc gaactggggg ttctggacct tgatgttcac
      541 gacggccaca agaccttcct tgatatcatc gccggtgagg ttctccttga ccttgccctg
      601 catgttactc tggatgaagt tgttcacgca tcgtgtcagg gcgctgcgga agcccgccac
      661 atgggtgccg ccttctttgg tgttgacgtt gttcacgaag ctctggatat tctcgttgta
      721 cccgtcgttg tactggaggg caacctcgag aagatcgagg gattcccgcg agttcgagac
      781 atatatcggt tcgtcgaaga gcacggtctt gctcgcatta aggtacttga cgaaggagcg
      841 gatgcctccc tcgtgcttga aatcctgctt tctggccttt ctctcatcat taaggatgat
      901 gtgaataccg ttgttgagga aggagatctc ccgcatccgg tgtgccagcg tatcgtagct
      961 gtattccgtg gtctcgaata tggtggcgtc ggccttgaac ttcaccttcg tgccggtgct
     1021 ctcggtgtct cctatgacgg tgaggtccgt catcctgtta ccctgttcgt agcgctggaa
     1081 atagacctgc cccgccctct tcacctctac ctcaaggtaa tgcgagagcg cgttgacgac
     1141 ggaaagacct accccgtgaa ggcctgctga ataccggtag gtgtccttat cgaacttccc
     1201 gcccgcgtgc agttttgtaa ggacgacctc gagggccgac atgttctcct cgctgtgcat
     1261 cgtcacgggt atacccctgc cgttgtcctc cacggtcacg ctgttgtcgc ggtgtatggt
     1321 caccgcgatc ctgttgcagt atccctccag cgcttcgtcc acgctgttgt cgacaagctc
     1381 gtagataagg tggtgcagcc cgtcgatgtt tgtattgccg atgtacatcg acggtacttt
     1441 cctgacggcg tcgagaccgc caagtatttt tatgctttcc gctccgtatt cacccatttc
     1501 tcatatcctt ataggcatca ctatgttctt gtagccgtcc agatccccgg acctgaagag
     1561 gacggcgcca tacgccttcg gcgcggctat tgtcacctgg tcacccgtca gatgtgttac
     1621 gacgtctatc aggaacttta cgttgaagtt cattgtcgtc tcttcaccat cgtatgttat
     1681 tccgatgacc tctttggccc tgccgatatc cgactccgcc tcgatcgtca tcgtattctc
     1741 ggacagtgtc atcttcaccg gctcagacct tccaatgatg gaagagacct tctttagccc
     1801 cttgaagagc aactctcttt ccacctccgc gacatttgtg ttgcccgagg ggaggacgtt
     1861 ctcataatcc ggaaaattcc cctcgatgat gcgggagatg atcttcatct tctccgtgct
     1921 gaactggacg tgtttttcat cgatgacgac acggacgtcc tcatcctctc ctatgaccct
     1981 ctccacttcc gaaatggacc tcttcgggat cgtcatcccc tgaaagggct taaggccgtc
     2041 gatctctttc tggtaaagtg ccatccggaa tccgtccgtt ccgacgacca ccaccttgtc
     2101 gtcctggcct ttcataaaca tcccggtgag tatatagcgg gtctcatcta tggaaacggc
     2161 gaactcgact ttgtctatca tctcaaggag gatgcttccg gggatggtg
//
LOCUS       DAOQTR010000393         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_61836_length_2208_cov_4.402229, whole genome shotgun sequence.
ACCESSION   DAOQTR010000393 DAOQTR010000000
VERSION     DAOQTR010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61836_length_2208_cov_4.402229"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKN85_09030"
     CDS             complement(<1..494)
                     /locus_tag="PKN85_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT44593.1"
                     /translation="MRINPRFINRFVRMGLNVQAYFTVGILIIIVAIIFFKGFPHVNF
                     AFIFSFPEDMGREGGIFPSIVGTIQLALLSILLATPLGLGTAVFLTEYTKESLFTKFI
                     RFGVESLAGIPSILYGLFGFIFFVIKLKMGWSLLAGVLTMTIMILPTIIRTSEEAIRA
                     VPRQL"
     gene            complement(491..1354)
                     /gene="pstC"
                     /locus_tag="PKN85_09035"
     CDS             complement(491..1354)
                     /gene="pstC"
                     /locus_tag="PKN85_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNT44594.1"
                     /translation="MIDRTVLKERFVKWVLVAFALSSLLFLFLIFIFILAEGLPLFLK
                     VGLNKIIFGMKWAPTKGHFGIFPMIISSFLVTIGALVIGAPLGLSCAIYLSEYAGKRT
                     RMLLKPAIELLAGIPSVVFGFLGVIYIVPLVRNYLGGSGFSLISTCLVLGVMILPTII
                     SISFDALVSVPRTYREGSAAMGATKWQTIYKVIIPSAKSGILASFILGMGRAIGETMA
                     VIMIAGNALKIPTSILDPLRTLTGNIALELAYATGDHRLGLFSTGVVLMVIIMILNYI
                     ANFGIKRKIAK"
     gene            complement(1347..2159)
                     /locus_tag="PKN85_09040"
     CDS             complement(1347..2159)
                     /locus_tag="PKN85_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT44595.1"
                     /translation="MNFRHWVILLPLIAALLSCSGASGDKPGKKHTITIAGSTSVMPF
                     TEKLAEHFMVDHKNFVIDVQGGGSTAGIQAVINNTVQIGMSSRQLKDDEKRLNTITIC
                     YDGIAVVVHPDNPVGEMDLDRIRQIFSGHISNWRQLGWVDRRIDAVSREEGSGTRGAF
                     EGLVMKDKEIDDGTMVQDSNGSVKEVVATDPYAIGYISMGLVDKKVKPIAIAGVKPSI
                     KTIKSGRYKLVRPFLYVTNGELDPRSRRFVDYVLSKEGQKILRNEGLVGFYD"
BASE COUNT          478 a          593 c          662 g          475 t
ORIGIN      
        1 agctgccgtg gcacggccct tatggcctcc tccgatgtcc ttatgatcgt cggcaggatc
       61 atgatggtca tcgtgaggac gcccgcgagg agcgaccaac ccatcttgag cttgatgaca
      121 aagaagatga agccgaagag gccatagagg atcgacggga tgcccgccag tgattcgaca
      181 ccgaaacgga tgaatttcgt gaagagcgat tccttcgtgt actccgtcag gaataccgcc
      241 gtcccgaggc ccagcggagt ggcgaggagt atggacaaga gggcgagttg gatcgttccc
      301 acgatggagg ggaagatgcc gccctccctc cccatgtcct cggggaaaga gaagatgaag
      361 gcgaaattga cgtgggggaa tcctttaaaa aagatgatcg ccacgatgat gatgaggata
      421 ccgacggtga agtatgcctg gacgttgagt cccatcctca cgaagcggtt tatgaaccgg
      481 gggttgattc tcattttgcg atcttcctct tgatgccgaa gttagcgatg taatttagta
      541 tcatgatgat gaccatgagg acgacgcctg tcgagaagag ccccaggcgg tggtcgccgg
      601 tggcatacgc gagctccagg gcgatgttgc ccgtcagcgt ccttaagggg tcgagaatgc
      661 tcgtgggtat cttgagggcg ttgcccgcga tcatgatgac cgccatcgtt tctcctatcg
      721 cccgtcccat gccgaggatg aagctcgcca ggatgcccga cttggccgag gggatgatga
      781 ccttgtagat ggtctgccat ttcgtcgccc ccatcgcggc ggaaccctcg cgataggtcc
      841 gggggacgct gaccagggca tcgaaggaga tgctgatgat cgtcggcagg atcatgacgc
      901 cgaggacgag gcaggtggat atgagagaga acccggaccc cccgagatag ttcctgacga
      961 ggggcacgat gtagatgaca ccgagaaacc cgaacaccac cgacgggatg ccggcaagca
     1021 attcgatggc gggcttaagg agcatccttg tcctcttccc cgcgtattcc gacaggtata
     1081 tcgcgcagga aagccccagg ggcgcgccga tgacgagcgc cccgatggta acgagaaacg
     1141 atgatatgat catcgggaat atgccgaagt ggcccttggt aggggcccat ttcatcccga
     1201 agatgatctt gttgaggcct actttgagga aaaggggcag gccctccgca aggatgaaga
     1261 tgaagatgag aaagagaaag agaagcgacg agagcgcgaa ggccacaagc acccacttga
     1321 cgaaacgctc tttcaatacc gtcctgtcaa tcatagaacc ccacgagtcc ttcgttcctg
     1381 agtatcttct gtccctcctt tgaaaggacg tagtcgacga accgccttga tctcgggtcc
     1441 agttcgccgt tggtcacgta aagaaaaggc cgcaccagct tgtaacggcc cgatttgatg
     1501 gtctttatgg aaggtttcac accggcgatg gcgatgggtt tgaccttctt gtcgacaagg
     1561 cccatggata tgtaccctat cgcgtaaggg tcggtggcga cgacttcctt cacggagccg
     1621 ttggaatcct gcaccatggt gccgtcatct atctccttgt ccttcatgac aaggccttcg
     1681 aaggcgcccc gtgtgcccga tccttcctcg cgggacaccg cgtctatcct cctgtcgacc
     1741 caccccagct gccgccagtt ggagatgtgg ccgctgaata tctggcgtat cctgtcgaga
     1801 tccatctcgc cgacagggtt gtcgggatgg acgacgaccg ctatcccgtc gtaacagatg
     1861 gttatcgtgt tgagtctctt ctcgtcgtcc ttcagttggc gggaggacat cccgatctgg
     1921 acggtgttgt tgatcaccgc ctggatcccc gctgtggagc cgcctccctg cacgtctatg
     1981 acgaagttct tgtggtccac catgaagtgc tccgccagtt tctccgtgaa gggcatgacg
     2041 gacgtggaac ctgctatcgt gatggtgtgt ttcttccccg gtttgtcacc ggaggcgccg
     2101 gagcaggaga gaagagcggc gataagaggt agaagtatga cccagtgcct gaaattcatc
     2161 tgttattcct ggggatctct ggagtgccgt attatctttc cctccacg
//
LOCUS       DAOQTR010000394         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_61851_length_2208_cov_3.122155, whole genome shotgun sequence.
ACCESSION   DAOQTR010000394 DAOQTR010000000
VERSION     DAOQTR010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61851_length_2208_cov_3.122155"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1075)
                     /locus_tag="PKN85_09045"
     CDS             complement(176..1075)
                     /locus_tag="PKN85_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT44596.1"
                     /translation="MSGTIGIKVDVDTFQGMRNGVPVLLDMFRRHDVKASFFVPMGKD
                     NTGRTVKRVFTRKGFLKKAGRVGVVSTYGVKTLLYGLVLPGPQIARRNITLIRKILND
                     GHELGIHGHDHVRWHDSIKDFDEAETRREVDRLLTVYRMVVGEEARSFAAPGWIINPF
                     ALKVFEEKGLVYSSDTRGVCPFYPEMGGSRFSLVQIPTTLPTLDEVIGVVSAEEKALV
                     DHYLTLLADGLNILTVHTELEGKRWSGFLDAFLSEAKQRGFTFRSLMEIAREFKDTPD
                     GPVCAIEYGSVPGRAGEVCCQGR"
     gene            complement(1082..2125)
                     /locus_tag="PKN85_09050"
     CDS             complement(1082..2125)
                     /locus_tag="PKN85_09050"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HNT44597.1"
                     /translation="MRLLVLGVNGFIGNALAEHVLNETDWEIYGLDIRNDKLGECLGH
                     ERFHYVEGDISINREWIEYHVKKCDVVMPLVAIATPMTYVREPLRVFELDFEENLKVV
                     RLCVKYGKRLLFPSTSEVYGMSPDAEFTEDGSSLVLGPINKQRWIYSASKQLLDRVIW
                     AYGFQHGLRFTLFRPFNWIGPKLDELATDPEKEGSSRVVTQFISSLFLAEPIRLVDGG
                     SQKRCFTYIDDGIACLMKIIENRDDRCNGQIFNIGNPRNEATVKDLAIKLKTMFAEDS
                     RTKHYTRHSEIIEVYSRDFYGEGYQDIDRRVPSIAKATEIVGWKPLVDLDTALRKTLD
                     YYLETSLPGEMGR"
BASE COUNT          454 a          648 c          619 g          487 t
ORIGIN      
        1 cacagaaaag aaatctgtca atcaaggaaa acccttaaga aaacgttcaa aggttcaagc
       61 gttcaagggt tcaagagaac aactggacca ccgcggagca cacctgcggc ccctcacacc
      121 ctttgaacct gtggagtttg ttaccctgga actcttgaac ctttgaaccc ttgaactatc
      181 ttccctgaca gcacacctcc ccggcgcggc cggggacgga accgtattct atggcgcaca
      241 cagggccgtc gggcgtgtct ttgaactcac gggcgatctc catgaggctt ctgaaggtga
      301 agccgcgttg tttggcctct gaaagaaaag catcgaggaa cccgctccag cgcttgcctt
      361 caagctcggt gtggaccgtc aggatgttga gaccatcggc caggagggtg agataatggt
      421 cgacgagggc cttttcctcg gcgcttacga ccccgatcac ctcgtcgagg gtcggaaggg
      481 tcgtcgggat ctggaccagg gagaaccggg accctcccat ttccggatag aaggggcaca
      541 caccccgcgt gtcactggaa tacaccaacc ccttttcctc gaagaccttg agcgcgaagg
      601 ggtttattat ccaccccggg gcggcgaagg aacgggcctc ttccccgacg accatccggt
      661 agacggtcag cagccggtcg acctcgcggc gtgtctcggc ttcatcgaaa tctttgatgg
      721 aatcgtgcca gcggacatgg tcatggccgt ggatgcccag ctcatggccg tcattgagaa
      781 tcttcctgat gagggttatg ttcctgcgcg cgatctgagg ccccggcaag acgaggccgt
      841 acaagagggt cttcacgcca tacgtgctca caacccctac gcgtcccgcc ttcttgagaa
      901 aacccttgcg ggtgaagacc cttttgacgg tccgacccgt attgtccttt cccatgggga
      961 cgaagaaact cgccttcaca tcgtggcgcc ggaacatgtc gaggaggacg ggcacgccat
     1021 tgcgcatacc ctgaaaagtg tccacatcga ccttgatccc tatcgtaccg ctcaacaacc
     1081 cctacctgcc catctctccg ggaaggctcg tctccaggta gtagtcgagg gtcttcctga
     1141 gggccgtgtc gaggtcgacg aggggtttcc agccgacgat ctccgtggcc ttcgctatcg
     1201 aggggaccct gcggtcgata tcctggtagc cttcgccgta gaaatcccgg gaatagacct
     1261 cgatgatctc ggaatgtctc gtgtagtgct tcgtcctcga gtcctccgcg aacatggttt
     1321 tcagcttgat ggcaaggtct ttcacggtcg cctcgttcct cgggttgccg atgttgaaga
     1381 tctggccgtt gcagcggtcg tccctgttct ctatgatctt catgagacac gcgatgccgt
     1441 catcgatata ggtgaagcac cgtttttgcg agcctccgtc gacaagccgg atgggttcgg
     1501 ccagaaagag gctgctgatg aactgcgtca ccacccggga gcttccctct ttttccgggt
     1561 ccgtggccag ttcgtcaagc ttcgggccta tccagttaaa gggccggaag agtgtgaacc
     1621 tgaggccgtg ctgaaaaccg tacgcccaga tgaccctgtc gagcaactgt ttcgacgcgc
     1681 tgtagatcca ccgctgcttg ttgatgggac cgaggacgag agaactgccg tcttcggtga
     1741 attccgcatc gggggacatc ccgtagacct ccgacgtgga cggaaagagg agtctcttgc
     1801 cgtacttgac gcaaagacgg acgaccttga ggttctcctc gaaatcgagt tcgaagacac
     1861 gaagaggctc cctcacgtat gtcatcggtg tggcaatggc cacgaggggc atgacaacgt
     1921 cgcacttctt gacgtggtac tctatccatt ccctgtttat cgagatatcg ccttcaacgt
     1981 agtggaaccg ctcgtggcca aggcactcgc caagcttgtc gttgcgaatg tccagcccgt
     2041 agatctccca gtccgtttcg ttgagcacgt gttccgcgag ggcgttgccg atgaatccgt
     2101 tcactccgag caccaagagt ctcaagggtg acctcctaga attttgtttt tcaagacacg
     2161 ggaagatgcc agttcgacgg cgtccatctc ctgctcacct tcgacctg
//
LOCUS       DAOQTR010000395         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_62068_length_2203_cov_3.540503, whole genome shotgun sequence.
ACCESSION   DAOQTR010000395 DAOQTR010000000
VERSION     DAOQTR010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62068_length_2203_cov_3.540503"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1022)
                     /locus_tag="PKN85_09055"
     CDS             complement(144..1022)
                     /locus_tag="PKN85_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44598.1"
                     /translation="MHDITENGRSKELLLKVRRFRAIFDHTFQFMGLLTPGGNVLEVN
                     GAALAFAGIREEEVVGRRFWLTPWWSHSRKLMNILRDAVKEAAGGRLMRFEATHLSSE
                     GRPHYFDFSLKPVPGEDGKVAFLIAEGRDVTDRKAVEEALRKREEELEFKTRNLEEAN
                     IALKALARHIEENRQEFERNIVLNVRTLVFPYIEKMQRSNLNIRQKMFMEVIEKNLGN
                     VISPFLKNISHFGLTPTEMHIAGLIRDGRKTKEIAESLHISRRAVEVHRYNIRKKLKM
                     NRSRQNLCAFLRSLGS"
     gene            1519..2151
                     /locus_tag="PKN85_09060"
     CDS             1519..2151
                     /locus_tag="PKN85_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44599.1"
                     /translation="MSEDHYRSLVDLSPDIIYRLDGEGRILFISNAVRELGYDPEELT
                     GRLFEDIVHPDDRGKGPVHFVERRVGSRSMQRLEMRLIKKSGAVQNYSLAQVTVELTA
                     RGKWDVPDSDISNPRKNLLYSQGVARDVTERKRMEVERDKLILELKEALLRVRTLSGL
                     LPICSSCKKIRDDDGRWQPLENYVREHSEAEFTHTICPECARKTYPNYFK"
BASE COUNT          476 a          648 c          616 g          463 t
ORIGIN      
        1 aaaagctgca ggcgggaccg gtgcaggagt tctcgggggg gttgtaggat gaccgggagg
       61 atgacctgcc gcggcaaacc ggaaacagcc cgctgtcacc ggtgaaagcg gagcgatccg
      121 ccgatgcggc gtctccgttc ggatcaactg cccagggagc ggaggaaagc gcacaggttc
      181 tgcctgctcc tgttcatctt cagtttcttc ctgatattgt agcggtggac ctcgacggcc
      241 cggcgggata tatggagcga ttcagcgatc tccttcgtct tccttccatc cctgatcagg
      301 cccgcgatat gcatctccgt gggtgtgagg ccgaagtggc ttatgttctt caggaagggc
      361 gagatcacgt tgccgaggtt cttttcgatg acctccatga acatcttctg cctgatgttg
      421 aggttcgatc tctgcatctt ctcgatgtac gggaaaacga gcgtcctgac gttgaggacg
      481 atgttcctct caaactcctg cctgttctct tcgatatgcc tcgcgagcgc cttcagggct
      541 atgttcgcct cttcaaggtt ccgcgtcttg aactcaagct cctcttctct cttcctcagt
      601 gcttcctcga ccgccttgcg gtccgttacg tcgcggcctt cggcgatgag aaaggcaacc
      661 tttccatcct cacccggcac gggtttcagc gaaaaatcaa aatagtgggg gcggccttcc
      721 gacgagagat gagtcgcctc gaagcgcata agccttccgc ccgccgcctc tttcacggca
      781 tcccggagta tgttcatcag ctttctggaa tgggaccacc acggcgtcag ccagaacctg
      841 cgcccaacaa cctcctcttc ccggatcccg gcgaaggcga gcgctgcccc gttcacctcc
      901 agcacgttgc cgccgggcgt caacaggccc ataaactgga acgtgtggtc aaatatcgcg
      961 cgaaatcttc gcaccttgag aaggagctcc ttcgaccttc cgttttcggt gatatcatgc
     1021 atggcccggg gtacgtccct ccgtttccgt acgtgatcat gatgcgcctt atacccgatt
     1081 agaataatag tcgaacatta tggggtttta cttaaaaggg gcgtgcccgg cgatagcgcg
     1141 cggactcccc tgaccccctt tccggggcgc catactacta gcaacgcgta cgtattccat
     1201 agcaagttat ttgctatagc attcttctgt ctttttgcat acaattacga atcacaacgg
     1261 cagggaacct taagggcgcg cggaaggcgc cgggtgtcgg aaggacaggg ggttcctttt
     1321 gatcggggga gttgaaggca ggcagtccca caccaccgac gagaagaacg gttttgtcat
     1381 gccgtccagc ccgttcgacc caaagtgatg ccaggggggc gagaacaccc tggcacgcca
     1441 ggttcgcccg caaacagaac agatgaaacc catcgcaatg gaaacagact atcgcatatg
     1501 ttcgggagga cctgtctctt gagcgaagat cactaccgtt ccctcgtgga cttaagcccc
     1561 gacatcatct accgcctcga cggcgaagga cggattctct tcatctccaa cgccgtcagg
     1621 gaactcggct acgacccgga ggagctcacg gggaggctct tcgaggacat agtccacccc
     1681 gacgaccgcg gcaaggggcc ggtccatttc gtcgaaagac gcgtcggctc gaggtccatg
     1741 caaaggctgg agatgcgcct gatcaagaag agcggggcgg tccagaacta ttcacttgca
     1801 caggtcaccg tggagctcac cgccaggggg aaatgggacg tccccgattc cgacatatcg
     1861 aatccgcgaa aaaatcttct ttactcccag ggcgtcgccc gcgacgtcac ggaacgcaag
     1921 cgcatggaag tggaacggga caaactcatc ctggaactca aagaggccct gctcagggtg
     1981 cggaccttga gcggcctctt gcccatatgc agctcctgca agaagatccg agacgacgac
     2041 ggacgctggc agccgctgga aaactatgtg cgggaacatt ccgaagccga gttcacccac
     2101 accatttgtc cggaatgtgc aagaaaaacg tatccaaact acttcaagtg agagggtcgc
     2161 ggtctccatc gcgtttcacg ttgaaggagt ctgaacaaga ggc
//
LOCUS       DAOQTR010000396         2198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_62297_length_2198_cov_3.204853, whole genome shotgun sequence.
ACCESSION   DAOQTR010000396 DAOQTR010000000
VERSION     DAOQTR010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62297_length_2198_cov_3.204853"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKN85_09065"
     CDS             complement(<1..675)
                     /locus_tag="PKN85_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44600.1"
                     /translation="MTPDELIARLRPHADALRKGAKSSIAGLGKVLGFNVLEGVIIPK
                     KCLCAAIGKGHVSVACGTRFLSRPRVVGFRRFSFEEGRYPRPEELASAVLSARDAFRA
                     RDAAMILSVPRPWVLTRVAELPSVVKDNVASVIAYELDRFTPFGSAEAMYDFAITSED
                     AEKLTIAIVAMRSETARPYLDALAAIGVSPQRVTTDTTAFGTLCSILGDARGMVLCLA
                     VDEEGYD"
     gene            complement(672..1628)
                     /locus_tag="PKN85_09070"
     CDS             complement(672..1628)
                     /locus_tag="PKN85_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNT44601.1"
                     /translation="MIRSRKGIALLMVLWVITMLMALVMSFSLLTRTEARSTIFFKEG
                     TQKKFLAEAGVERAVMEIYHRQTFRNQAVAIEGNEVVRIDGRQYSARIGQGSYNFRLL
                     SESGKIDINKMTDLTGIVLKNLLVNTGMTDEEAAVIVDSILDWADKDNLRRLNGAEDE
                     YYLSLPVPYRAKNRPFDAIEELLLVKGMSVDILFGTGERRGIIHYLTIYGTSSKVNIN
                     AAPKEVLIALPGLTEETVTRILDQREAAEFTSVQEIQAITGLNFPTLAQYIDMSETDV
                     YTIESVGFQGDSKDGYGIRAIVSVPGTVPRFLYYKTPSEMHR"
     gene            complement(1625..>2198)
                     /locus_tag="PKN85_09075"
     CDS             complement(1625..>2198)
                     /locus_tag="PKN85_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44602.1"
                     /translation="LLEVMISITLISILVVIIAGAMRLANRSVERGERRSEELERFKV
                     SFLVMDAQIQSAIPLVRADDEAGRLFFEGSGDTVKFASNYSLTGGYRGYVTVTYRVRP
                     NGEGRNSLYIEENTIGLENTREMRLLDGLDEIRFEYFRKENDADDTTGEWVETWTDEL
                     LFPRRIRVHVARAGQKIQLTVPVRARKAAS"
BASE COUNT          414 a          689 c          628 g          467 t
ORIGIN      
        1 gtcgtatcct tcttcatcga cggcaaggca caggaccatg cccctcgcgt cccccaggat
       61 ggagcagagc gtgccgaaag cggtcgtgtc cgtcgtcacc ctctgagggc tcacaccaat
      121 tgccgccagg gcatcgagat aaggcctcgc cgtttcggac ctcatggcga cgatggcgat
      181 cgtgagcttc tccgcgtcct ctgacgttat cgcaaaatcg tacattgcct cggcggagcc
      241 aaagggggtg aatctgtcga gctcgtaggc aatgaccgaa gccacgttgt ccttcaccac
      301 cgaggggagc tcggcaaccc tcgtcaggac ccatggccgg gggacggaga ggatcatcgc
      361 cgcgtcacgc gccctgaaag cgtcccgggc ggagaggacc gccgatgcca gctcttccgg
      421 gcggggatac cttccttcct cgaaactgaa gcgtctgaac cccacgactc tgggtcgcga
      481 caggaatcgt gttccgcagg cgaccgagac atgacccttc ccgatggcgg cgcagagaca
      541 ctttttcggg atgatgacgc cctcgaggac attgaagcca aggacctttc cgaggcccgc
      601 gatagacgac ttcgccccct ttctcagggc gtcggcgtgc ggcctcaacc gtgcaatcaa
      661 ttcatcgggc gtcatcggtg catctccgaa ggcgtcttgt agtacaggaa ccggggtacc
      721 gtgcccggca cggacacgat ggcccggatc ccgtacccat ctttcgaatc tccctgaaaa
      781 cccacggatt ctatcgtgta gacatctgtc tcgctcatgt ctatgtattg cgcaagggtg
      841 gggaaattga gtcccgttat ggcctggatc tcctggacgg acgtgaactc ggccgcctcc
      901 cgctggtcca ggatcctcgt gacggtctcc tccgtcaggc ccggcagggc tatgaggacc
      961 tctttcgggg ccgcattgat gttcaccttc gaggacgtcc cgtatatcgt caggtaatgg
     1021 atgatccccc tgcgttcccc ggtcccgaag aggatatcga cggacatacc cttcaccagc
     1081 aggagctcct cgatggcgtc gaagggcctg ttcttggccc tgtagggaac ggggagagaa
     1141 aggtagtact cgtcttccgc tccgttcagg cgccggaggt tgtccttgtc cgcccaatcg
     1201 agaatggagt cgacgatgac ggcggcctcc tcgtcggtca ttcccgtgtt gactagaagg
     1261 ttcttcaaaa cgatgccggt gagatcggtc atcttgttga tgtcgatctt gcccgattcg
     1321 gaaaggagcc tgaaattgta gctgccctgc ccgatccggg cgctgtactg cctgccgtcg
     1381 atcctcacca cctcgttccc ttcgatggcc acggcctggt tccggaaggt ctgcctgtga
     1441 tagatctcca tgacggccct ctccaccccg gcctcggcaa ggaacttctt ctgcgtccct
     1501 tccttgaaga agatcgttga acgggcctca gtcctcgtca ggagagagaa ggacatcaca
     1561 agggccatga gcatcgtgat cacccacagg accatgagaa gggctatgcc tttccgggag
     1621 cgtatcatga ggccgccttc ctggccctca ccggcacggt cagctggatc ttctgacctg
     1681 cccgggcgac gtggacccgg atcctccggg ggaagagcag ttcgtcggtc cacgtttcga
     1741 cccattcccc cgtggtatcg tcggcgtcgt tctccttgcg gaaatactcg aagcgtatct
     1801 catccagccc atcgagaagc ctcatctccc tggtattttc cagccctatg gtgttctcct
     1861 cgatgtacag ggaattcctg ccttccccgt tcggcctcac ccggtaggtc acggtgacat
     1921 accccctgta tcctcccgtc agcgaatagt tcgaggcaaa tttcacagtg tctcccgacc
     1981 cttcgaaaaa caggcggccc gcttcatcgt cagccctgac gaggggtatt gccgattgta
     2041 tctgcgcatc catgacgagg aaagagacct tgaaccgttc gagttcctcc gacctgcgct
     2101 cgcccctttc gacggaacgg ttggcgaggc gcatggcccc ggcaatgatc accacgagga
     2161 tgctgataag ggtgatggag atcatgacct cgaggagt
//
LOCUS       DAOQTR010000397         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_62452_length_2195_cov_2.735981, whole genome shotgun sequence.
ACCESSION   DAOQTR010000397 DAOQTR010000000
VERSION     DAOQTR010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62452_length_2195_cov_2.735981"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="PKN85_09080"
     CDS             complement(<1..1741)
                     /locus_tag="PKN85_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44603.1"
                     /translation="MKRGRVIVYPAVADAVDLPDMTFLWGGDGDAFVPSATVEGLLSG
                     KNAAVADRMKGLDDTRLKTLLGALLPEWAGSTVTVTITGRRSPLTKISLKGKNVAVQV
                     HWNLFVPYFDHIKSLPVVHPEILDVFAMAVFRYMALMMKDHEVANDEIRDRVTEAYKE
                     EPGLLLASLDIFNKPTIYHIRPTRVWLNKIFSANDAYRLEIDVSDAADAPFVSAEFLD
                     ELTCFIHKVVKNYAFRYGDYLWTQGYRITLGARSGQGMVTCEHRDIVINIHPLIYQSD
                     WRPVVYLNLGYAIVLAMLRSPSGGSEEKRTHLALMKTWNRYLSFDESSERPGVRELCG
                     FSPSTNEQECRSLSECMVGRQDRESVDDFIMLQYHSSRESFPEKPEGPDEGQSGKGPA
                     YFERLSNTVRELNTTLMRNNINHGKLIEILRSDGFDHLRLAEFLRTGRLDNRCVVDVS
                     EWLLLRTFLYDLINAPKVFKNDYKMLLRVLTKVNKSLLVYIWTLGLEKDPRFEMVSAY
                     ISKVMHDIFVFDRVKIYEIVSDPAECLEAEAVTGYLLNWAGFTPEVRRSIIELLSGVP
                     DHVVRQLRVKTTDD"
     gene            complement(1965..>2195)
                     /locus_tag="PKN85_09085"
     CDS             complement(1965..>2195)
                     /locus_tag="PKN85_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44604.1"
                     /translation="GLIGGAVGVAFASFMQLVEVSTVNFQTFSELAFKFSLTPGITMR
                     ALVFAIVMGLAGGFLPALRAAHMNIVEALRSK"
BASE COUNT          458 a          699 c          591 g          447 t
ORIGIN      
        1 catcgtccgt cgtcttcacc ctcaattgcc ggaccacatg gtccggaacc cccgacagca
       61 actcgatgat cgatcgccgc acctcgggtg tgaaccctgc ccagttgaga agataccccg
      121 taacggcctc ggcctcgaga cactccgccg ggtccgagac gatctcgtat atcttcacgc
      181 gatcgaagac aaagatatcg tgcatcacct tcgatatgta ggcgctcacc atctcgaaac
      241 gcgggtcctt ctcgagaccc agcgtccaga tatagacaag gaggctcttg ttcactttcg
      301 tgaggaccct cagcagcatc ttgtagtcgt tcttgaagac cttcggcgcg ttgatgaggt
      361 cgtacaggaa cgtcctcaac agcagccatt cgctgacatc cacgacgcat ctgttgtcga
      421 gacggcccgt gcgcaggaat tcggccagcc gaaggtggtc gaagccgtcg ctgcgcagga
      481 tctcgatcag ctttccgtga ttgatgttgt tccgcatcag ggtcgtgttg agctcccgga
      541 ccgtgttcga cagccgctcg aagtaagcgg gccccttccc gctctgtcct tcgtcgggac
      601 cttccggttt ctccgggaaa ctttcccgtg aggaatggta ctggagcatg atgaaatcgt
      661 cgacgctctc ccggtcctgt cgaccgacca tgcactccga gaggctgcgg cattcctgtt
      721 cgttcgtcga tggggaaaac ccgcacagct cgcgcacacc gggccgttca gaggattcgt
      781 cgaagctcag gtagcggttc caggtcttca tgagggcgag gtgcgtgcgt ttctcttcgc
      841 tccctcccga cggcgagcgg agcatggcga gcacgatcgc ataccccagg ttcagataga
      901 ccacgggccg ccaatcggac tgatagatca ggggatggat gttgataacg atgtccctgt
      961 gctcgcaggt caccataccc tgtcccgaac gggcgcccag ggtgatcctg tacccctgcg
     1021 tccagaggta atccccgtaa cgaaaggcat agttcttcac gaccttgtgg ataaaacacg
     1081 tgagctcgtc gagaaactcc gcgctcacga agggggcgtc cgccgcgtcg gagacatcga
     1141 tttccagacg ataggcgtcg ttcgccgaga agatcttgtt caaccacacc cgcgtcgggc
     1201 ggatatggta gatggtgggc ttgttgaaga tgtccagcga tgcgaggaga agtcccggct
     1261 cctccttgta cgcttcggtc acgcggtccc ttatctcgtc gttcgccacc tcgtggtcct
     1321 tcatcatcag ggccatgtaa cggaaaaccg ccatggcaaa aacatccagt atttcgggat
     1381 gaacgacggg aagcgacttg atgtggtcaa aatagggaac aaacaggttc cagtggacct
     1441 ggacggccac gttcttcccc ttcagactga tcttcgtgag cggtgaacgg cggccggtga
     1501 tcgtgacggt cacggtcgag cccgcccatt cagggaggag cgctccaagg agcgtcttca
     1561 accgtgtatc gtcgaggccc ttcatcctgt ccgccacagc cgcgttcttc ccggaaagaa
     1621 gtccctccac cgtggccgac gggacaaagg catctccatc cccgccccac aggaacgtca
     1681 tatcaggcag gtcgacggcg tcggcgacgg ccggatacac gatcaccctg ccccgtttca
     1741 ccgatgctcc cccgcgctct caagcacaac cattctctct accccctttt ttggacgcat
     1801 cccccagtat agcacgaacc actccccaat ttcccaccca aatcacccca accatacctt
     1861 tagacctggt cagtcccctc tttggccttt gactcgaaac gcgcaactcg gaacccgcaa
     1921 ctacgtattg cttgaaatct gaaaccctga acctggaacc gtcttcattt gcttctcaac
     1981 gcctccacga tgttcatatg cgccgccctc agggccggca ggaagcctcc cgcaaggccc
     2041 atgacgatcg cgaagacaag ggccctcatc gtgatgccgg gggtgaggga gaacttgaag
     2101 gcaagctccg agaaggtctg gaagttcacc gtggacacct ccacaagctg catgaaagag
     2161 gcgaacgcta ctcccaccgc cccgccgatg aggcc
//
LOCUS       DAOQTR010000398         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_62747_length_2188_cov_3.204876, whole genome shotgun sequence.
ACCESSION   DAOQTR010000398 DAOQTR010000000
VERSION     DAOQTR010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62747_length_2188_cov_3.204876"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1376)
                     /locus_tag="PKN85_09090"
     CDS             complement(177..1376)
                     /locus_tag="PKN85_09090"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNT44605.1"
                     /translation="MKKVKKVVLAYSGGLDTSILVKWLKDVYNCEVIAYAADVGQEEE
                     LVGLEEKALKTGASKVYIEDLREEFARDFIFFAIKANAIYEGNYLMGTSIARPLIAKR
                     QIEIARKERADAVCHGATGKGNDQVRFELTFYAMEPNIRIIAPWREWEFTSRDDLIDY
                     AKKHGIEVPVTKKKPYSMDRNLMHISYEGGILEDPWSEPMENMFKTTVSPEKAPNKAA
                     YVEITFEKGVPVAIDGKKMSAFKIIDHLNKIGGKNGIGRVDIVENRFVGMKSRGVYET
                     PGCTILHAAHRAVETLTLDREVMHIRDGLIPKVAELIYYGFWYSPEMKAIMALTDEIQ
                     SVVNGTARLKLYKGNCIVVGRKSKNSLYMKDFATFDTDTVYDQKDAGGFIRLNALRLR
                     IRAMLEK"
     gene            complement(1389..>2188)
                     /gene="argF"
                     /locus_tag="PKN85_09095"
     CDS             complement(1389..>2188)
                     /gene="argF"
                     /locus_tag="PKN85_09095"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNT44606.1"
                     /translation="AMIFEKASTRTRLSFEAGMHQLGGQAIFLSPSETQIGRGEPIQD
                     TARVLSRYVDGVMIRTFSQGIVEDLARWASIPVINGLSDQYHPCQTLADLQTIQEYKG
                     GLRKVKVAYIGDGNNVANSWLEAAILMKMPFAIATPKGYLPDGELIERASAGTGLTIT
                     DDPAEAVRGADVIYTDVWVSMGQEKEKASRKRAFKGYRIDDKLLRLAHRDAIVMHCLP
                     AYRGQEITDEVFERFQEVIFTQAENRLHAQKALLEWLLAGRVKASAQ"
BASE COUNT          390 a          674 c          591 g          533 t
ORIGIN      
        1 cttccccgag ggataaggga acatctcaag gagatagtat ttatgtttcg acgggtcttc
       61 atcgacacgg aacgcttctc gctcttccca gtatttttgc catttctctt cgatcgcatg
      121 aggctcatag gggttcatca tgttctccaa acgtgtctat cgataagggg gacctttcac
      181 ttctccagca tcgcccggat gcgcagtctc aacgcgttga gacgaatgaa accgccggca
      241 tccttctggt cgtacacggt atcggtgtcg aaggtggcga aatccttcat gtaaagagaa
      301 ttcttcgact tgcggcccac gacgatgcag ttgcccttgt agagcttcaa ccgcgccgtc
      361 ccgttcacca ccgactgtat ctcgtccgtg agcgccatga tggccttcat ctccggcgag
      421 taccagaacc cgtaatagat gagttcggcc accttgggga tgaggccgtc gcggatatgc
      481 atcacttccc tgtccagggt cagggtctcc actgcccggt gcgccgcgtg aaggatggtg
      541 catcccggag tctcgtacac gccccgtgac ttcatgccca cgaaacggtt ctccacgatg
      601 tcgacgcggc cgatgccgtt cttccctccg atcttgttca ggtgatcaat gatcttgaac
      661 gccgacatct tcttgccgtc tatcgccacg ggcacgcctt tctcgaaggt tatctccacg
      721 tatgcggcct tgttgggggc cttttcaggg gagaccgtcg tcttgaacat gttctccatc
      781 ggctccgacc aggggtcctc cagaatgccg ccctcatagc ttatatgcat gaggttccgg
      841 tccatgctgt acggcttctt cttcgtcacc ggcacttcta tgccatgttt cttcgcgtag
      901 tcgatgaggt cgtcgcggga ggtgaattcc cactccctcc agggtgcgat gatcctgatg
      961 ttcggctcca tcgcgtaaaa ggtgagctcg aaccggacct ggtcgttgcc cttgcccgtc
     1021 gccccgtggc agacggcgtc ggccctctcc ttgcgggcta tctcgatctg cctcttcgcg
     1081 atgagcggcc tcgcgatgga ggtgcccatg aggtagttgc cctcgtagat ggcgttggcc
     1141 ttgatggcaa agaatatgaa atccctcgcg aactcttcac gaaggtcctc tatgtatacc
     1201 ttggaggctc ccgtcttcag ggccttctct tccagaccga caagctcttc ctcctgtccg
     1261 acatccgcgg cgtaggctat gacctcgcag ttgtagacat ccttgagcca ttttacaaga
     1321 atcgatgtgt cgagccctcc cgaatacgcg aggaccactt tcttaacctt tttcatcctg
     1381 acctccgttt attgtgccga agccttcacc cgtcccgcga gtagccactc gaggagcgcc
     1441 ttctgcgcat gcagcctgtt ctccgcctgc gtgaatatga cctcctgaaa gcgttcgaag
     1501 acctcgtccg ttatttcctg gccgcgatag gcaggcagac agtgcatgac gatggcgtcc
     1561 ctgtgtgcca gcctcaagag tttgtcatca atcctgtaac ctttaaaggc cctcttcctc
     1621 gaggccttct ctttctcctg ccccatgctg acccacacgt cggtatatat gacgtcggcg
     1681 ccgcggacgg cctcggccgg gtcgtcggtt atggtgaggc cggtacccgc cgacgctctc
     1741 tcgatcagtt ccccgtcggg caggtagcct ttcggcgtgg cgatggcaaa gggcatcttc
     1801 atcaggatcg ccgcctccag ccacgagttg gcaacgttgt tcccgtctcc gatgtaggcc
     1861 accttcacct ttctgagacc ccccttgtat tcctgtatgg tctgaaggtc cgcgagggtc
     1921 tggcaggggt ggtactggtc ggaaagaccg ttgatcacgg gaatgctcgc ccacctggcg
     1981 aggtcttcga cgatgccctg cgagaaggtg cggatcatga cgccgtcaac ataccggctc
     2041 agaacgcgag ccgtgtcctg tatcggctct ccccggccga tctgcgtctc ggagggagag
     2101 agaaagatgg cctgcccgcc caactgatgc atgcccgcct cgaaggagag acgagtccgc
     2161 gtggaggcct tctcgaagat catggcaa
//
LOCUS       DAOQTR010000399         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_62808_length_2187_cov_2.340056, whole genome shotgun sequence.
ACCESSION   DAOQTR010000399 DAOQTR010000000
VERSION     DAOQTR010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62808_length_2187_cov_2.340056"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKN85_09100"
     CDS             complement(<1..349)
                     /locus_tag="PKN85_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44607.1"
                     /translation="MKVAAAYPGTPSSEILKDAADTYPEIYAEWSPNEMVAVQVASGA
                     ALAGARAMASMKHVGMNVAADSLMTLAYTGIKGGLMIVVADDPNVHSSQNEQDSRNWA
                     RFGKVPMLEPDDAQ"
     gene            complement(557..1870)
                     /locus_tag="PKN85_09105"
     CDS             complement(557..1870)
                     /locus_tag="PKN85_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT44608.1"
                     /translation="MKTFDDAVSIIREFLRIDTTNPPGKEEAAAHFLEDILRREGLDS
                     EIHSAAPTRANIISRIKGREEGKPIVLLGHTDVVPAKEEEWTAHPFGGEVKDGYVYGR
                     GAIDMKAQVICQLIAFVDLAHRGVVPKRDIIFLATCDEEVGGANGMQFMLDRIPELKD
                     ASFVLSEGGCLIEENGLVHAQVSVTEKKLSQFVVKAKGTGGHGSMPHGDNANEKIVEA
                     SHRILSHAWPLKAMPITSAYLGGILGERTLGGAKFPGVKEALKTPKWRRLLEGNEIYN
                     AILRNTVTLTILKGGEKVNVIPAESSATFDARLLPAETHERFFRKVRRLAGKDVVIER
                     IGEKISEPGPSRYNTVYFQGIRNCVTSLKGQVPVLPFVTTGATDLRYFRDIGVPAYGF
                     FPITLTKEEHMRMHGKDERISLENIREGLEGCRGIVNFLASPSVP"
     gene            complement(1854..>2187)
                     /locus_tag="PKN85_09110"
     CDS             complement(1854..>2187)
                     /locus_tag="PKN85_09110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT44609.1"
                     /translation="GDQVLKGITRTMKDNLRNIDRLGRYGGEEFLVILPETGLDKALV
                     VAERLRAAIGSAVIDSGDDRIEVTVSAGLASYTPGRLEKDLIKEADDNLYRAKAEGKN
                     RTYYEDFR"
BASE COUNT          381 a          677 c          576 g          553 t
ORIGIN      
        1 cctgggcgtc gtccggttcc agcatgggta ccttgccgaa acgggcccag ttgcggctgt
       61 cctgctcgtt ctgggaactg tggacgttgg gatcgtcggc cacgacgatc atgagaccgc
      121 ccttgatacc cgtgtaggca agggtcatca gggaatctgc tgcgacgttc atgccgacgt
      181 gcttcatgga tgccatcgct ctcgcgccgg caagggccgc acccgatgcc acctgaaccg
      241 ccaccatttc gttgggtgac cattcagcat agatctccgg gtaggtgtcg gcagcgtctt
      301 tgaggatctc actgctcggt gttccgggat atgcggcagc gactttcaca ccagcttccc
      361 aggcacctct tgcaattgct gcgtttcctt gcattatttc tttcatacta cccccataat
      421 ggcgtatcgc ctggattttg agtgactaat tacttcacct cctttggaac gatttttctt
      481 gtattttaac taaggcttgt taatgaattc acgtattatt ttcgacagat taaacgtcct
      541 ttgtcaagaa atatcgttac ggaacgctgg gagacgccag gaaatttacg atgcccctgc
      601 acccttccaa tccttcccta atattctcaa gggatatcct ctcatccttg ccgtgcatcc
      661 tcatgtgttc ttccttcgtg agggtgatgg ggaagaaccc gtaggccgga actccgatgt
      721 ccctgaagta tctgaggtcc gtcgcgcccg tcgtgacaaa gggaaggacg gggacctgtc
      781 ctttcaatga agtcacgcaa ttccttatgc cttgaaaata gaccgtgttg taccgtgagg
      841 gcccgggttc gctgatcttc tctccgatac gctctatgac gacgtccttt cccgcgagcc
      901 gcctcacctt cctgaagaac ctctcgtggg tctccgcggg aaggagcctg gcgtcgaagg
      961 tcgccgagga ctcggccggt atgacgttga ccttttcgcc tcccttaagt atggtgagcg
     1021 tcaccgtgtt cctgaggatg gcgttgtata tctcattgcc ctcgaggagg cgcctccact
     1081 tcggtgtctt cagggcctcc ttcacgccgg ggaacttcgc tccccctagc gttctttcgc
     1141 cgagtatgcc tccgagatag gccgaggtta tcggcatcgc cttgagaggc caggcgtggg
     1201 agagtatccg gtgcgaggct tcgacgatct tttcgttcgc gttatctcca tgaggcatgg
     1261 acccatgccc cccggtgcct tttgccttca cgacgaactg actcagcttc ttctccgtca
     1321 ccgagacctg ggcatggacc agcccgttct cctcgatgag gcagccgcct tcgctcagga
     1381 caaaggacgc gtccttaagc tccggtatcc tgtcgagcat gaactgcatc ccattggctc
     1441 cgcccacctc ctcgtcgcac gtggcaagga aaatgatgtc cctcttcggg acaacgcccc
     1501 ggtgagccag gtctacaaag gcaatgagct gacatatcac ctgcgccttc atgtcgatcg
     1561 ctcccctgcc gtagacatag ccgtccttca cctccccccc gaagggatgg gccgtccatt
     1621 cctcctcttt ggcggggacg acgtcggtgt ggcccaggag aacgatcggt ttcccctctt
     1681 ctcttccctt tatccgggag atgatgttgg ccctcgtagg cgcggccgaa tgtatttcgg
     1741 aatccagccc ttcccgcctc aggatgtcct cgagaaagtg cgctgccgct tcctctttcc
     1801 ccggagggtt cgtggtgtct atgcgcagaa attccctgat gatcgagacg gcgtcatcga
     1861 aagtcttcat aatatgtcct gttcttccct tctgcttttg cccgatagag gttgtcgtcg
     1921 gcctccttga tgaggtcctt ttccagtcgt cccggcgtgt aggaggccag ccccgcgctc
     1981 accgtgacct cgatccgatc gtcgcccgag tcgatgacgg cactgccgat cgccgcgcgc
     2041 aatcgttccg ccacaaccag ggccttgtcg aggccggtct ccggaaggat gacaagaaac
     2101 tcctctccgc cgtacctgcc cagcctgtcg atgttcctca ggttgtcctt catggtccgc
     2161 gtgatgccct tcaggacctg gtctcct
//
LOCUS       DAOQTR010000400         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_62955_length_2183_cov_4.316729, whole genome shotgun sequence.
ACCESSION   DAOQTR010000400 DAOQTR010000000
VERSION     DAOQTR010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62955_length_2183_cov_4.316729"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKN85_09115"
     CDS             complement(<1..630)
                     /locus_tag="PKN85_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNT44610.1"
                     /translation="MIHIHILLFLATIASTALVGGFLYSLAIMTILLCHELGHYLVGR
                     HYGIPSTLPFFIPLPLPPFGTFGAVIKMKGGFVNRSTLFDVGIAGPLSGFAAALPFIV
                     AGMKMSTVMPLAGIPSASYMQLGDPLLFRVLQPLLVGDIPADHDLVLHPFAYAGWVGL
                     FVTSLNLLPVGQLDGGHPFPVVGHGAHGLAAGVQAQPGQPGRQRRAQGEF"
     gene            complement(638..1600)
                     /locus_tag="PKN85_09120"
     CDS             complement(638..1600)
                     /locus_tag="PKN85_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44611.1"
                     /translation="MNKLLVAMGAKENVVEDIHVLARRFREHPGIVIAQGKDLRQVVI
                     EKASNLFLADELVIAIVDPTAQDVAVLRPHLDALKERVGVLVYFTSDAANLAESLGGK
                     VIALEKDREKRIRQRVLSILKTNGKKMTDKAFSLLTSRVRDESILEQEVLKLVGYAGE
                     RATIESRDIRAVVTETHEETLMALFDAFSRMDKKEVLTIFEDLLENGQHILAIHSYLV
                     NQMRLLIQAKDLEPFVRDAKAYPAFAAEFKRWKERVDVDTSGRKRYLPFQHPYYAFKL
                     TGTSRKISRESLVEFYDFLSSLDAKIKTGTKQERTLMEFGLLKV"
     gene            complement(1658..>2183)
                     /locus_tag="PKN85_09125"
     CDS             complement(1658..>2183)
                     /locus_tag="PKN85_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44612.1"
                     /translation="RSFSDRTRYAVIDIGVLIHQIPGGMISNLVSQLKQAKALDRLEE
                     VYEEISRTRQDMGFPPLVTPTSQIVGVQAVFNVIAGRYKMISNEVKDYFYGLYGRPPA
                     PVNEEIKAKALKGYPRGATPIDVRPADILEPELPAAREALKGISEDMGDILIYALYPV
                     TGLEFLKKKFGKQE"
BASE COUNT          455 a          631 c          639 g          458 t
ORIGIN      
        1 aaactctccc tgggctcttc gctgtcgtcc tggctgaccc ggctgggcct ggacaccggc
       61 ggcaagacca tgcgcgccgt ggccgacaac tgggaacgga tggcctccat caagctggcc
      121 gacgggcagg aggttgaggg acgtgacgaa aagccccacc cagcctgcgt aggcaaaagg
      181 atggaggacg agatcgtggt cagcggggat atcgccaacg agaagtggct gaagcaccct
      241 gaaaaggagg gggtccccca gttgcatgta actcgcggag ggtataccgg cgagaggcat
      301 cacggtggac atcttcatgc ccgccacgat aaagggcagg gcggccgcga atccgcttaa
      361 ggggcccgcg atgcccacgt cgaaaagcgt gctcctgttg acaaaaccac ctttcatttt
      421 tattacggcc ccgaatgtgc cgaagggggg caaaggcagg ggtatgaaaa aagggagcgt
      481 gctcgggatg ccgtaatgcc tgcccacaag gtaatgcccc aattcgtggc agaggaggat
      541 cgtcatgatg gccaggctgt aaaggaaacc accaaccagc gccgtcgatg ctattgtcgc
      601 cagaaaaaga aggatatgaa tgtggatcat cggggatcta gaccttcagg agcccgaact
      661 ccatgagcgt gcgctcctgc ttcgtgcccg tctttatctt cgcgtcaaga gagctcagga
      721 agtcgtagaa ctcgacaaga ctctcccggg agatcttccg agatgtgccc gtgagcttga
      781 aggcataata agggtgttga aacggcaggt agcgcttcct gcccgatgtg tccacgtcca
      841 ccctctcctt ccatcgcttg aattccgcgg caaaggcggg gtatgccttc gcatcccgca
      901 caaaaggctc caggtccttt gcctggatca gaaggcgcat ctggttcaca agatagctgt
      961 gtatggcaag gatgtgctga ccattctcga gaaggtcttc gaagatggtg aggacttcct
     1021 tcttgtccat tctcgagaag gcgtcaaaga gggccatcaa agtctcttcg tgcgtctccg
     1081 tcacgacggc gcggatatcc cgggattcga tggtcgccct ttcgcccgca taccccacca
     1141 acttgaggac ttcctgctcc agtatggact catccctgac cctcgaagtt aagaggctga
     1201 atgccttgtc cgtcatcttc ttgccgttgg tcttgaggat actgaggacc cgctgtcgta
     1261 tcctcttttc cctgtccttt tccagggcga tcaccttccc tcccaggctt tccgcgaggt
     1321 tggcggcatc ggacgtgaag tagacgagca cacccaccct ctccttcaga gcatcgaggt
     1381 ggggccgcag cacggccaca tcctgcgccg tcgggtcgac tatggcgata acgagctcgt
     1441 cggcaaggaa caggttggat gccttctcga tgacaacctg cctgaggtcc ttaccctgcg
     1501 cgatgacgat cccgggatgt tcgcggaacc gtcgggccag gacatggatg tcctcgacaa
     1561 cattctcctt cgcgcccatg gcaacgagca atttgttcat ggggttaaga taccatcggg
     1621 gagaaggctt tgcaagagga agaaagacgg gggatcctca ctcctgcttc ccgaacttct
     1681 tcttcaggaa ttccaggccc gtcacgggat agagggcgta gatgaggatg tcgcccatgt
     1741 cctccgatat gccttttagc gcctcccggg ctgcgggtag ctccggctcg aggatgtccg
     1801 ccgggcgcac gtcgatgggc gtcgctcccc tggggtagcc cttcaatgcc ttcgccttta
     1861 tctcctcgtt cacgggcgcg ggcggacggc cgtaaagacc gtagaaatag tccttcacct
     1921 cgttgctgat catcttatac cggcccgcga tgacgttgaa gacagcctgc actccgacga
     1981 tctggctcgt gggcgtgacg agcggcggaa agcccatatc ctgcctcgtt ctcgatatct
     2041 cttcgtagac ctcctcgagc ctgtcgagcg cttttgcctg cttgagctga ctgaccaggt
     2101 tcgagatcat gccaccgggg atctgatgga tgaggacccc gatatcaata acggcataac
     2161 gggtccggtc tgaaaaagat cgc
//
LOCUS       DAOQTR010000401         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_63408_length_2174_cov_3.039641, whole genome shotgun sequence.
ACCESSION   DAOQTR010000401 DAOQTR010000000
VERSION     DAOQTR010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63408_length_2174_cov_3.039641"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PKN85_09130"
     CDS             complement(<1..853)
                     /locus_tag="PKN85_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNT44613.1"
                     /translation="MPTDLLLNMRLYVARRLTPARIFILGFISIILAGTVLLLMPSSV
                     HQGRLGLVDALFTSTSAVCVTGLTVIDIGRDLSLSGQTITLFLMQAGGLGITTFSVLF
                     LGMMGFGISAKSKDIVQSTFTHSPGIDFFHILRSVVVYTAVIEAAGTAILFLCFVEGS
                     DPAAAIWRAAYHAISAFNNCGYSIFPDSLMSYRDHLGVNLTVMALILVGGIGFIVIHE
                     VLEHGREQRKRLSVHTRIVVITTSILIVAGAVLIYTLERKFMLKGLGLESQVLVPLFQ
                     SVTARTCG"
     gene            complement(965..>2174)
                     /locus_tag="PKN85_09135"
     CDS             complement(965..>2174)
                     /locus_tag="PKN85_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNT44614.1"
                     /translation="RFLWHEGITGFFVLSEVILCATGGEALYADMGHLGRKPIIRAWY
                     FVFFALALSYLGQGSFILGHQGGASNALFGMILSQSVVLYVPFLILSIIATVIASQAM
                     ISGMFSIVYQGINTRLLPMFKVEYTSPRLRSQIYIGFVNWFLLLSVLFIMVEFRESHR
                     LAAAYGLAVTGTMALTGIMMTAIFHLRREWFKMTVAIMVTIVDLVFLVSNTYKIPHGG
                     YWSIIIVAIPLLVMLVYTAGQRRLYRRIRPLPLDIFLESYNQVYRELNKIGGTALYFA
                     RDPQMIPPYIVHTMFRNHIIYETNVIVSIKTLDQPFGVQSSFQETLADGLEAFKIEMG
                     YMEVVKIEEIMKAAGINEKVIFYGLEEITTNRPLWKVFATLKRLTPPFIQFHDLPLNK
                     LHGVITRVEM"
BASE COUNT          497 a          576 c          690 g          411 t
ORIGIN      
        1 agccgcaggt cctcgccgtg accgactgga acagagggac gagcacctgc gactcaaggc
       61 caagaccctt cagcatgaat tttctctcca gggtatagat caggaccgcg cccgccacga
      121 tgaggatgga cgtcgtgatg acaacgatcc tggtatgcac cgagagtctc tttcgctgct
      181 ccctgccgtg ctcgaggacc tcgtggataa cgatgaagcc gatcccgccg acaaggatga
      241 gggccatgac cgtcaggttg acgcccaggt gatcgcggta ggacatcagg ctgtccggga
      301 agatcgaata cccgcagttg ttgaacgccg atatcgcgtg gtaggcggcc ctccagatgg
      361 ccgcggcggg atcggacccc tccacgaaac agagaaagag gatggccgtc ccggccgctt
      421 cgataacagc cgtgtagacg acgacagacc ggaggatgtg gaagaagtcg atgcctggag
      481 aatgggtgaa tgtcgactgg acgatatcct tgctcttggc cgagatcccg aaccccatca
      541 ttcccaggaa aaggacggag aacgtcgtga taccgaggcc gcccgcctgc atgaggaaga
      601 gcgttatcgt ctggccgctc aaggagaggt ccctgcctat gtctatgacg gtaaggccgg
      661 tgacacagac ggccgatgtc gatgtgaaga gggcgtcgac gagaccgagc cggccttggt
      721 ggacgctcga cggcatgagc agcaatacgg tcccggcgag gatgatggaa atgaatccga
      781 ggatgaatat gcgcgcgggc gtgagacgcc gggcgacata cagcctcatg ttaagcagga
      841 ggtcggttgg catggtcttt acgtgctggg tgaaatgtta tcccattatc gccgacgatt
      901 gcaacagata tgacggcggg ccgacggcgc tccttcgctg tatgggaccg atgttcccgg
      961 gggctcacat ttcgacgcgc gtgatcacgc cgtggagttt gttcaggggg aggtcgtgga
     1021 actggatgaa gggcggcgtc agcctcttca gggtcgcgaa gaccttccac aggggcctgt
     1081 tcgtcgttat ctcctcaaga ccgtaaaaga tgaccttctc gttgatcccc gcggccttca
     1141 tgatctcttc tatcttgacg acctccatgt aacccatttc gatcttgaac gcttcaagcc
     1201 cgtcagccag cgtctcctgg aaggaactct gcaccccgaa gggctgatcg agggtcttta
     1261 tggagacgat cacgttcgtc tcgtagatga tgtggttccg gaacatggtg tggacaatat
     1321 aaggaggtat catctggggg tcacgggcaa agtagagggc ggtcccaccg atcttgttga
     1381 gttcgcggta gacctggttg tagctctcaa ggaatatatc cagcggcagg ggccgtatcc
     1441 ggcggtagag cctgcgctga ccggccgtgt acacgagcat gacaagaaga ggaatggcga
     1501 cgatgatgat ggaccagtac ccgccgtggg gtatcttgta ggtgtttgaa acaaggaaca
     1561 cgaggtcgac gatcgtgacc atgatggcga cggtcatctt gaaccattcc cggcgcaggt
     1621 ggaatattgc cgtcatcatg atccccgtca gcgccatggt gcccgtgacg gccagtccgt
     1681 aagcggcagc aaggcggtgc gactcgcgga actcgaccat tatgaagagg accgagagaa
     1741 ggaggaacca gttgacgaaa ccgatgtata tctgcgaacg cagcctgggc gaggtgtatt
     1801 cgaccttgaa catgggaagg agccgggtgt tgatcccctg gtagactatg gagaacatcc
     1861 cgcttatcat ggcctgcgac gcgatgaccg tggcgatgat gctcaggatg aggaagggca
     1921 cgtacaaaac aacggactgg ctgaggatca tcccgaagag cgcgttcgat gcgccgccct
     1981 gatgcccgag gatgaacgaa ccctggccga ggtagctcaa ggccagggca aagaagacga
     2041 aataccaggc acgtatgatg ggcttgcggc caaggtgccc catgtccgca tacaacgcct
     2101 caccacctgt cgcgcagagg atgacctctg agaggacgaa gaagccggta atcccctcgt
     2161 gccagaggaa tctc
//
LOCUS       DAOQTR010000402         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_63495_length_2172_cov_4.392537, whole genome shotgun sequence.
ACCESSION   DAOQTR010000402 DAOQTR010000000
VERSION     DAOQTR010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63495_length_2172_cov_4.392537"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1771)
                     /locus_tag="PKN85_09140"
     CDS             complement(125..1771)
                     /locus_tag="PKN85_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT44615.1"
                     /translation="MSLKIGTKFMLFISGVIAVAVVITASACLWEMRNDLIRQANRTM
                     DSRLNVFWELLNAKSQSSMEAAQSGQKQKVPFTIEDDRLVVGAYGLNGDTVLVDKIKE
                     FFGGTATIFMRDVRVSTNVLNPDGTRAVGTRLKGPVHDAIFQKKTSFRGSADILGKSY
                     FVAYDPIRGQNGEVIGVLYVGMPKGDYFAAFNRILMFVVIIGIILIGAVCVMSYFYMR
                     KLTLPLNECVSAAQRLAEGDLTVDIKAEDESETGQLLKGMQNMVLHWREIVHEVKEAT
                     DRIIGESRQLSVHADQMQQGSAQQATKSSQVASSAEEMSQTIVDVARNTNDIATSAGM
                     AVGIAKSGADVVSQSIEEVRSIAEVVRRSGQFVESLGRRSEQIGEIIVLINDIADQTN
                     LLALNAAIEAARAGEVGRGFAVVAEEVKKLAEKTAAATLEIGQTIEGMRSEVENATRA
                     MEEADVKVGSGVELVSRTGGSLGEIVSSAEGLQTMVMQIASATEQMSAASEEINREIV
                     DIAAVTNETSRSSEMTAASASSLLGLSDRLQNLMGRFRVS"
     gene            1967..>2172
                     /locus_tag="PKN85_09145"
     CDS             1967..>2172
                     /locus_tag="PKN85_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT44616.1"
                     /translation="MRCDNCGNESPEGSRFCIKCGREFGASGSGITVCPHCGVQIAPG
                     SLFCSACGKSIGAPQGEVNQGRTS"
BASE COUNT          414 a          681 c          581 g          496 t
ORIGIN      
        1 cttcttatca tggacggcct gtgatattcc atggggagta acatacaagg tttcaagcca
       61 aagacattag aatgttcaac tgtctttaag cctgaaacgt gaaatctgga acgtggaacc
      121 atctttaact caccctgaac cggcccatca ggttctgcag cctgtctgag aggccaagga
      181 ggcttgatgc cgaggccgcc gtcatttcag agcttctcga ggtttcgttc gtcacggcgg
      241 ctatgtcgac gatctcgcgg ttgatctcct ccgatgcggc ggacatctgc tccgtcgcgg
      301 aggctatctg catgaccatg gtctggaggc cctcggcact gctcacgatc tcacccaggg
      361 accctcccgt ccgggagaca agctccacgc cggagccgac ctttacgtcg gcctcttcca
      421 tagctctcgt ggcgttctcg acctctgagc gcatcccctc gatcgtctgg ccgatctcca
      481 gggtggcggc cgccgtcttc tcggccagct tcttaacctc ttcggccacg acggcaaacc
      541 ctctgcccac ctctcctgca cgggcggctt cgatcgccgc gttgagagcg agaaggttcg
      601 tctgatcggc tatatcgttg atgagaacga tgatctcgcc tatctgctcg gaccgtcttc
      661 ccagcgactc cacgaactgg cccgaccttc tcaccacctc ggcgatactc ctgacctcct
      721 cgatggattg gctcaccaca tcggctccgc tcttcgcgat gccgacggcc attcccgccg
      781 acgtcgctat gtcattcgtg ttccgtgcca cgtccacgat ggtctgcgac atttcctccg
      841 cggacgaggc cacctgtgaa gatttcgtcg cctgctgtgc cgagccctgc tgcatctggt
      901 ccgcgtgcac gctgagctgc cggctttcgc cgatgatgcg gtccgtcgct tccttgacct
      961 cgtgcacgat ctcccgccag tgaagcacca tgttctgcat ccccttgaga agctggcctg
     1021 tctcactttc gtcctcggcc tttatgtcca cggtcaggtc gccttcggcc agtctttgcg
     1081 cggcgctcac gcactcattg aggggaaggg tcagtttgcg catgtagaag taggacatga
     1141 cgcacaccgc accgatcaat atgatcccga tgatgacgac gaacatgagg atgcggttga
     1201 atgccgcaaa atagtcgccc ttgggcatac ccacgtagag cacgccgatg acctctccgt
     1261 tctggccgcg gatggggtcg taggctacaa agtaggattt ccccagtatg tccgcgctgc
     1321 cgcggaagga cgttttcttc tggaagatgg catcatggac ggggcctttc aggcgcgtcc
     1381 ccaccgcccg cgtgccgtcg ggattgagga cattcgttga gacgcggaca tccctcatga
     1441 agatggtggc cgtcccgccg aagaattcct tgatcttgtc gacgagcaca gtatcgccgt
     1501 tcagaccgta ggctccaacc acgagcctgt cgtcctcgat cgtgaacggg accttttgtt
     1561 tctgcccgga ctgagcggcc tccatggatg actgcgactt cgcattcagg agctcccaga
     1621 acacgttgag tcgtgaatcc atcgtcctgt tcgcctgccg tatcagatca ttccgcatct
     1681 cccacaggca cgctgatgca gttatcacaa ccgccacggc aatgacaccg gagatgaaaa
     1741 gcatgaattt ggtgccgatc ttgagactca ttctcatgga ccaccctctc cccagaatat
     1801 tcaacaacct gctgatctat ccaatgtata tgtctatccc gattatcgaa atttgcggcg
     1861 gtgacttaag cgtgatccct cgacgtcgcg caatgatccc cgcactttcc ctttacaatc
     1921 tccggggccg ctggtacaat cgctaaaatt ccggaggagg tacatcatgc ggtgtgacaa
     1981 ctgtggtaac gaaagccccg agggatcacg gttctgcata aaatgcggaa gggagttcgg
     2041 tgcctccggc tcggggataa cggtctgtcc tcactgcgga gtccagatcg ccccggggtc
     2101 tctgttctgc tccgcctgcg gcaaatccat aggcgccccg cagggggagg tgaaccaagg
     2161 aaggacgtcg ca
//
LOCUS       DAOQTR010000403         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_63987_length_2162_cov_3.099193, whole genome shotgun sequence.
ACCESSION   DAOQTR010000403 DAOQTR010000000
VERSION     DAOQTR010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63987_length_2162_cov_3.099193"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1370
                     /locus_tag="PKN85_09150"
     CDS             201..1370
                     /locus_tag="PKN85_09150"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNT44617.1"
                     /translation="MGGRKRIAVLTGGGDVPGLNVAIKAVVARADSRDIEVVGIRRGW
                     MGLLCIDPGSPETAQSLVMPLTLDKVRTIDRTGGTILHTSRTNPGNVNADEVPPFVEE
                     GDRVPTASGRIDCTRHILKVLESLAIDAIIPIGGDDTLSYAVRLHKEGVQVVAIPKTM
                     DNDVFGTDFCIGFSTAVTRSVDAINALRTTTGSHERICVVELFGRNSGETSLIAAYLA
                     DVDRAVISEVPFDLDKLVGFLVTDRSRNPSNYAIMTISEGAHTVGGTVTETGIEDAYG
                     HKKLGGVGYLVSQEIKQRSGVNVIYQQLAYMMRSGAPDSLDRMVAVSYGSLALDQIAM
                     GHRGRMVALQQAIYTTVPLDMVVAARKQVDVTALYDVDTYRPKVRDTLGKPMFLY"
     gene            1643..1975
                     /locus_tag="PKN85_09155"
     CDS             1643..1975
                     /locus_tag="PKN85_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44618.1"
                     /translation="MALIDDIRKRTEEGLKTLKETAQDIAFSVEKQAMIGKRKYLDVA
                     KLQRSIQKVNAEIGEYVYDQFTCNKSVSSDDPFIRDRIGSITRMRLTIRDIEQEIAEL
                     ETTKPPKR"
BASE COUNT          483 a          586 c          652 g          441 t
ORIGIN      
        1 aagttcaaga gtaaaagaca aaaagggcac cggcgccttc ggccggtgta cgaatccccc
       61 ttgacagccg accccccctt cgcggtcttt cccctgaacc gttgaacggt ttttggtcct
      121 tccttgacat tcccttcttt cgcgactata cagaacttta tggaaaacgc gtgaccccga
      181 cggggatggg aggtgttgtt atgggaggga ggaagagaat tgccgtgctg acggggggag
      241 gggatgttcc cggcctcaat gtggccataa aggccgttgt cgcgcgagcc gattcgagag
      301 acattgaggt ggtcggtatc cgcaggggat ggatgggttt gctctgcatc gatcccggca
      361 gtcccgagac ggcacagagt ctcgtcatgc cgctcactct cgacaaggtc aggaccatcg
      421 atcggaccgg cgggaccatc cttcacacat cacgtaccaa tccgggaaac gtgaacgccg
      481 acgaggtgcc ccccttcgtg gaggagggtg accgggtccc gacggcatcg ggaaggatcg
      541 actgcacgag gcatatcctg aaggtcctcg aatccctggc gatagatgcc atcatcccca
      601 tcggcgggga cgacaccctg agctacgcgg tccgactcca caaggaaggc gtgcaggttg
      661 tcgccatccc gaagaccatg gacaatgacg tcttcggaac cgatttctgc ataggttttt
      721 cgacggccgt gacacggtcc gttgacgcca tcaacgccct gcgcaccacg acgggttccc
      781 atgaacgcat ctgcgtcgtc gagctatttg gcaggaattc cggcgagaca tcgcttatcg
      841 ccgcctacct ggcggacgtc gacagggccg tcatatcgga agtgcccttc gacctcgaca
      901 agctcgtcgg ttttctggtg acggaccgat ccagaaaccc ctcaaactat gctatcatga
      961 ccatctccga aggggcccat acggtgggcg ggacggtgac ggagaccggt atagaggatg
     1021 cctacggcca caagaagctt ggcggggtgg ggtacctcgt ttcccaggag atcaagcagc
     1081 gctcgggtgt aaacgtcatc taccagcagc tagcatacat gatgcggtcc ggggcccccg
     1141 attccctcga ccggatggtc gccgtatcct atggcagcct ggctctcgat cagatagcaa
     1201 tgggccacag gggccgtatg gtggcactgc agcaggcgat atacaccacc gtaccgctgg
     1261 atatggtcgt cgcggccagg aagcaggtgg atgtgacggc cctgtacgac gtggacacgt
     1321 accgtccaaa ggtccgcgac acgctgggga aaccgatgtt tctctactag aggcggcttg
     1381 aatcgcttga gagtcaaagg caaaagacgg gaaaggcggc ttacgtagct tgaatcgtct
     1441 gagtatgaag gcaagacgac tggagatggt tgggttggag aagactctgg tcccggcttt
     1501 gccgggcgga cgaaccgtcc ggtcctacga ggttctttaa cattcaagcc attcaagccg
     1561 cctcttaagc cgcctttgtg tttgaccttt ggcgcctcaa ggtgcagaat attgaaaaat
     1621 ccatgttgga tcggaggttg tggtggcgct tattgatgat atacggaaga ggaccgagga
     1681 agggttgaag acgctgaagg agacggcgca ggatatcgcg ttcagtgtgg agaagcaggc
     1741 gatgatcggg aagcggaagt acctcgacgt cgcaaagctg cagcgttcca ttcagaaggt
     1801 gaacgctgag ataggtgagt acgtgtacga ccagtttacc tgcaacaaga gcgtttcctc
     1861 cgatgacccc ttcatccgtg accgcatcgg ctccatcacg cggatgcggc tcaccatacg
     1921 cgacatcgag caagagatag ccgaactgga gaccacgaaa ccacccaaga gataaagaca
     1981 gtcccaggtt ccaagtctca ggtttcaggt aaaagacagt tgcgggttcc gggttgcgtg
     2041 tttcgagtca aaaccccggg agggatggca ctcttctact ccaagggttt ttcatctgag
     2101 acctgaggcc tggaacctgg gacctttttt tgcagcagtt ccaggagttc cctcatttct
     2161 tc
//
LOCUS       DAOQTR010000404         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_64023_length_2161_cov_3.564577, whole genome shotgun sequence.
ACCESSION   DAOQTR010000404 DAOQTR010000000
VERSION     DAOQTR010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64023_length_2161_cov_3.564577"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKN85_09160"
     CDS             <1..286
                     /locus_tag="PKN85_09160"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNT44619.1"
                     /translation="SRRVKTCFACDLISEMLLYLKPHSLLITSLTNAHVIHAAQVMDA
                     KAVLFVGGRRPDESVVRSGELNKIPLLTTMLLTFDCCGRLYEKGIRGDKQ"
     gene            309..776
                     /locus_tag="PKN85_09165"
     CDS             309..776
                     /locus_tag="PKN85_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44620.1"
                     /translation="MVDSLAYTPQITLEFEIGEKDFFVAGECASRVKKTLQQLGLKQD
                     VIKRIAIIIYEAAMNVAIHATSGRLVVHVEPDAITIRTEDVGAGIEDIDLAMKEGYST
                     ASYEIREMGFGAGMGLSNIKQCSDDLNIESRVGVGTTLNARVFFSDERRHEGA"
     gene            773..2131
                     /locus_tag="PKN85_09170"
     CDS             773..2131
                     /locus_tag="PKN85_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNT44621.1"
                     /translation="MNRYFHSVTLETEKCKGCTNCIKRCPVEAIRVRDGKAMIVQERC
                     IDCGECIRECPNHAKVAVTDTLDKLSEYKYRIALPAPSFFGQFKEQVNIEDVLGAFKG
                     IGFDEVFEVALAAEIVGYIVHEFLKDYKGKRPIFSSACPAVVRLMQIKYPDLLEQMAP
                     FLTPMEVAARIAKEQASRRTGIPYEEIGAFFISPCPAKVTEMRNPMTTKHSAVNGVIG
                     INLIYKDIIRHLGDRSANGGPEKLQRATKFGMAWGYVTGESRNIDAGTTLAVGGIHNV
                     ISLLEEIERGELKDVDFVEVQACTGGCVGGPLNIQNLFVGRIRLRDLIKRFGTKEPYF
                     TEAELQDIYLQNRFAATEPVEPRAIMTLDEDVSRAIMKMERLDQITSELPGLDCGACG
                     SPSCRALAEDIIRGIAFETDCVIKLREKVKILAKEILDLSRIVPTSMSGSSKEGDPGQ
                     GH"
BASE COUNT          487 a          590 c          670 g          414 t
ORIGIN      
        1 ttcacgcagg gtgaagacct gtttcgcctg cgacctcatc agcgagatgc tcctttacct
       61 gaagccccat tcgcttctga tcacgtcgtt gaccaacgcg catgtgatcc atgcggcaca
      121 ggtcatggac gcgaaggcgg tcctctttgt cgggggacgc aggcccgatg aatccgttgt
      181 caggagcggg gaattgaaca agatccccct gctgaccacg atgcttctca cgttcgactg
      241 ctgcggccgg ctttacgaga agggtatcag aggagataag caatagcccg gttaggagag
      301 actaaccaat ggttgacagc ttagcataca ccccgcagat cacgctcgag ttcgagatag
      361 gcgaaaagga tttctttgtt gccggagagt gtgcgtctcg ggtgaagaag acccttcagc
      421 agcttgggct caaacaggat gttatcaagc ggatcgcgat catcatatat gaggcggcga
      481 tgaacgtggc catccacgcc acgtcgggaa ggcttgtcgt ccatgtcgag cctgacgcga
      541 tcaccatacg gacagaagat gtgggggccg ggatagaaga tatcgacctt gccatgaagg
      601 aagggtattc gacggcctcc tacgagatcc gggagatggg gtttggggcc ggcatgggcc
      661 tttccaacat caaacagtgc tccgatgacc tcaatatcga atccagggtg ggcgtgggaa
      721 cgactctgaa cgccagggtc ttcttcagtg atgagaggcg gcacgagggc gcatgaaccg
      781 atacttccat tccgtgacgc tggagaccga aaagtgcaag ggatgcacca attgcatcaa
      841 gcggtgtccc gtcgaggcta tccgtgtccg tgacggcaag gcgatgatcg ttcaggaacg
      901 ctgcatcgac tgcggcgaat gcatccgcga atgcccgaac cacgcgaaag tggcggtaac
      961 ggacacactc gataaacttt cggaatacaa gtaccggatc gccctgccgg cgccgtcgtt
     1021 cttcggccag ttcaaggaac aggtgaatat cgaggacgtg ctcggggctt tcaaggggat
     1081 cggtttcgat gaggtctttg aggtcgccct tgccgcggag atcgttggct acatcgtcca
     1141 cgagttcctg aaggattaca agggcaagcg ccccatattc tcctctgcct gcccggcggt
     1201 ggtgcgcctc atgcagatca agtatcccga ccttctcgag cagatggccc ccttcctcac
     1261 acccatggag gtggcggcgc gcatcgcaaa ggaacaggca tcgcgcagga cgggcatccc
     1321 ctacgaggag ataggggcat tcttcatatc gccctgtccg gcgaaggtca cggagatgag
     1381 aaatcccatg acgacgaagc actcggcagt gaacggtgtc atagggatca accttatata
     1441 taaggatatc atcaggcatc tcggcgacag gtcagcaaac ggtgggcccg agaaactgca
     1501 gcgtgccacc aagttcggca tggcctgggg atacgtgacg ggagagtccc ggaacattga
     1561 cgcgggcacc actctggccg tcggcggcat ccacaacgtt atcagcctcc tggaagagat
     1621 cgagaggggc gagctcaagg acgtcgattt tgtcgaggtt caggcgtgca ccggcggttg
     1681 cgtgggaggg ccgctcaaca tccagaatct cttcgtgggg cgtatccggc tccgcgacct
     1741 cataaagcgg ttcggaacga aagagcccta tttcacggag gccgaacttc aggatatcta
     1801 tcttcagaac cggtttgccg ccacggagcc cgtggagcca agggcgatca tgaccctcga
     1861 tgaggacgtc tcccgggcga tcatgaagat ggagcgcctg gaccagataa cgagcgaact
     1921 gccgggtctc gattgcggcg cctgcggctc cccaagctgc cgggcccttg ccgaggacat
     1981 catccgcggc atagccttcg agaccgactg tgttatcaag ctgcgcgaga aggtgaagat
     2041 actggcaaag gagatcctcg atctttcccg catcgttcca acctcgatga gcggcagttc
     2101 gaaagagggc gaccccggac aggggcactg acgagtgtcg ggacggtcag ggggatgctg
     2161 g
//
LOCUS       DAOQTR010000405         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_64077_length_2160_cov_3.125416, whole genome shotgun sequence.
ACCESSION   DAOQTR010000405 DAOQTR010000000
VERSION     DAOQTR010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64077_length_2160_cov_3.125416"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /gene="lpxD"
                     /locus_tag="PKN85_09175"
     CDS             <1..720
                     /gene="lpxD"
                     /locus_tag="PKN85_09175"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNT44622.1"
                     /translation="PEAEVAASASVGPFACIERQARIGEKVTVYPFCYIGPNVTIGDG
                     SVIYPHVTIYRDTTIGRNVIIHANTVIAADGFSYTWDGKRHAKIPQIGSVIIEDDVEI
                     GANTDIDRAALDATVVGRGTKIDNLVQVAHNVTIGQNSIIVSQVGIAGSAHIGNNVVL
                     AGQTGVKDHVSVGDQVMAGGRTGITKDVPAGSLIWGTPHMPHKEYMRLQMYIKKLPAL
                     FEKIRELEKRLSPEEDDDRHK"
     gene            704..1132
                     /gene="fabZ"
                     /locus_tag="PKN85_09180"
     CDS             704..1132
                     /gene="fabZ"
                     /locus_tag="PKN85_09180"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNT44623.1"
                     /translation="MIDINEILSLMPHRYPFLLVDRVIEFEETKRIVAIKNVTINEPF
                     FQGHFPGRPIMPGVLILEAMAQAGGVLAFKSFPGMKGTVFFTGIDGARFRKPVIPGDQ
                     LRFVVDVVKHRREIWVFEAKAYVGDDVVAEARIMAMLKQE"
     gene            1144..1911
                     /gene="lpxA"
                     /locus_tag="PKN85_09185"
     CDS             1144..1911
                     /gene="lpxA"
                     /locus_tag="PKN85_09185"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNT44624.1"
                     /translation="MIHPTAIVDSSAEIAGDVEIGPYCIVEGGVRIGAGSRLESHVVV
                     QGNTVIGEGCTVSPFASIGGPPQDIGYKYEPTRVVIGRNNTIKEYVTINRGTMKGGGV
                     TEVGENNFIMAYAHIAHDCKVGNNIIMANGATLAGHVEVSDFVIFSGLCAVHQFCRVG
                     KYAFISGLTGIPKDVPPFVIAAGDRAKLYGLNLVGLERRNFSKDEIMKLKKAYRILFR
                     SALPLTTSLKIIREELDGDNIGYLIEFIESSTRGICR"
     gene            1902..>2160
                     /locus_tag="PKN85_09190"
     CDS             1902..>2160
                     /locus_tag="PKN85_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT44625.1"
                     /translation="MPVNVALIGLGHMGRIHLGKLCSFEDVRVCGVVDVDDALAGEYA
                     GKYTVPSFSRYSDILPDAACAVIATPTQSHYAIAKACLERGV"
BASE COUNT          485 a          656 c          624 g          395 t
ORIGIN      
        1 cccgaggccg aggtcgccgc cagtgcctcc gtcggcccct tcgcctgcat agagcggcag
       61 gcaaggatcg gggagaaggt gacggtctat cccttctgct atatcggtcc caacgtcacc
      121 atcggcgatg gcagcgtcat ctatccccac gtcaccatct accgggacac gacgatcggg
      181 agaaatgtca tcatccacgc gaacaccgtc atagccgccg acggcttcag ctacacctgg
      241 gacgggaaaa ggcacgccaa gatcccccag atcggctccg tcatcataga ggacgatgtg
      301 gagatcgggg ccaataccga tattgaccgc gcagcgcttg acgccacggt ggtgggcagg
      361 gggacgaaga tcgacaacct cgttcaggtc gcccacaatg ttactatcgg acagaactcc
      421 atcatcgtct cccaggtcgg catagcgggc agcgcgcaca tagggaacaa cgtcgtcctc
      481 gcgggccaga cgggggtcaa ggaccacgtc agcgtgggcg atcaggtcat ggcgggggga
      541 cggacgggga tcacgaaaga cgttcccgcc gggtccctca tatgggggac cccgcacatg
      601 ccccacaagg aatacatgag actccagatg tacataaaga agctccccgc cctttttgaa
      661 aagatcaggg aactggagaa gagactctca ccggaggaag acgatgatcg acataaatga
      721 gatactgagc ctgatgcccc acagataccc ctttctcctc gtcgaccgtg tcatcgaatt
      781 cgaagagaca aagaggatag tcgccataaa gaacgtgacc atcaacgagc ctttctttca
      841 gggtcacttc ccgggaaggc ccatcatgcc cggcgttctc atccttgaag cgatggcgca
      901 ggcaggcggc gtccttgcct tcaagtcctt ccccggcatg aagggtaccg tcttctttac
      961 cggcatcgac ggggcacggt tccgcaaacc ggtgatcccc ggcgaccagc tcaggttcgt
     1021 tgtcgacgtc gtgaaacaca gacgggagat ctgggtattc gaggcaaagg cctacgtcgg
     1081 agacgatgtc gtcgccgagg cgcgtattat ggccatgtta aaacaggagt gagggagact
     1141 tacgtgatac atccaaccgc catcgttgac agcagcgccg agatcgcagg agatgtcgag
     1201 atcggtccct attgcatagt tgaagggggc gtgcgcatcg gcgcgggcag ccgcctcgag
     1261 agccatgtcg tcgttcaggg gaacaccgtc ataggcgaag gctgcaccgt gagccctttc
     1321 gcgtccatcg gaggacctcc ccaggacatc ggctacaagt acgaacccac ccgggttgtc
     1381 ataggccgca acaacaccat aaaggaatac gtcaccatca accggggaac catgaaaggg
     1441 ggcggcgtca ccgaggtggg ggagaacaat ttcatcatgg catacgctca catcgcccac
     1501 gactgcaagg tggggaacaa tatcatcatg gcgaacgggg caaccctcgc gggccatgtg
     1561 gaggtttccg attttgtcat cttcagcggc ctctgcgccg tccaccagtt ctgcagggtc
     1621 ggcaagtacg ctttcatcag cggtctgacg gggatcccca aggatgtgcc tcccttcgtc
     1681 atcgccgccg gcgacagggc gaaactctac ggcctcaacc tcgtgggcct cgaaaggcgc
     1741 aatttctcca aggatgagat aatgaagctg aagaaggcgt accgcatcct cttcaggtcc
     1801 gctctccccc tcacgacatc cctcaagatc atcagggagg agctggacgg ggacaatatc
     1861 gggtatctca tcgagttcat agaatcgtca acgaggggga tatgccggta aacgtcgccc
     1921 tcatcggtct cggccacatg ggccgcatcc atctcggcaa actttgctcc ttcgaggacg
     1981 tccgcgtctg cggggttgtc gatgtcgatg acgctctcgc cggcgaatat gccgggaaat
     2041 acacggttcc ctccttctcc cgttattccg atatcctgcc cgacgcggcc tgcgccgtca
     2101 tcgccacacc gactcagtcc cactacgcca tcgcgaaggc ctgtctcgaa cggggcgtcc
//
LOCUS       DAOQTR010000406         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_64205_length_2157_cov_3.800190, whole genome shotgun sequence.
ACCESSION   DAOQTR010000406 DAOQTR010000000
VERSION     DAOQTR010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64205_length_2157_cov_3.800190"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="murB"
                     /locus_tag="PKN85_09195"
     CDS             <1..782
                     /gene="murB"
                     /locus_tag="PKN85_09195"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNT44626.1"
                     /translation="ISAMVLRLKDSGIPYRFLGNGTNIIVSDEGIRAALIRITKMTRL
                     TFEKTRDGAIAEVGGGYPLARFIRECAKRGLGGLERLYWIPGSVGGAIKMNAGSFDQS
                     ISDTVIGMDVLTARGEIIEKAVKFEDFGYRTSPVGRQECVITGRFRLKAAGVEDLEEQ
                     MEHVYRERKERHPMEYPSAGSVFKAAKGRSAWWFVEKAGLRGYRIGDACVSEKHTNFI
                     VNMGHARATDIAALIRKVKEEVFSKLGVTLEEEVELWGFNG"
     gene            775..1683
                     /locus_tag="PKN85_09200"
     CDS             775..1683
                     /locus_tag="PKN85_09200"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNT44627.1"
                     /translation="MDRTGLKKLKIGVLLGGRSSEREISLKSGAAVLQSLVRSGYDAV
                     AIDARDRLVEKLKKQKVDAAFIVLHGRWGEDGTVQGLLEIMGIPYTGPGVLGSSAAMD
                     KAVMKFILEATGIPTPAYAIAGAGERITMKPPFVVKPANEGSTIGISMVHRRKDTAAA
                     MELALRYDRKIVIEEFVPGQEITVAVVNGKVLPVVEVRPLSGFYDFESKYTKGKTEYI
                     VPAEIPRKVAKKAEAIAMDVYRRFDLSGCVRTDMLVRDGMPLVIDINTSPGMTETSLV
                     PKAWACQGGSFDELIEEILGGASLKT"
     gene            1680..>2157
                     /locus_tag="PKN85_09205"
     CDS             1680..>2157
                     /locus_tag="PKN85_09205"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNT44628.1"
                     /translation="MKKLLYILFIGPVCILSMLSIAYLFSREEPLFFLQNIKVNGISQ
                     LGDKETMAKIAPHLTESIFKVDVAKVRDALTSHPFVKEVSVKRVYPFSIVIDVKEKIP
                     SALWVAGDGTVHILDEKGEPYRGLGKDEVKGLYLISTRERSDAKKVFSETNVFTVGT"
BASE COUNT          490 a          606 c          696 g          365 t
ORIGIN      
        1 atatctcggc gatggtgctg cgcctcaaag actcgggcat cccttaccgc ttcctgggca
       61 acggaacgaa catcatcgtc agcgacgaag ggatacgggc ggcgctgata cggataacga
      121 agatgacacg gctgaccttc gagaagaccc gcgacggcgc catcgccgag gtgggcggag
      181 ggtaccccct tgcgaggttc atcagggaat gtgcgaagcg gggcctcgga ggcctggagc
      241 ggctgtactg gataccggga agcgtgggcg gggccataaa gatgaacgcc gggagcttcg
      301 accagtcgat atccgacacc gtcatcggca tggacgtcct tacggcgcgc ggcgagatca
      361 tcgaaaaggc ggtcaagttc gaggatttcg ggtaccgaac ctcaccggtc ggccggcagg
      421 agtgcgtcat caccggcaga ttccgtctta aggccgccgg cgtggaggac ctcgaagagc
      481 aaatggaaca cgtataccgg gagagaaagg aacgccaccc catggagtat ccctccgccg
      541 ggtccgtctt caaggcggcc aagggcaggt ccgcgtggtg gttcgtggaa aaggcgggtc
      601 tcagaggata ccgcataggc gatgcctgcg tctcggagaa gcacacgaac ttcatcgtga
      661 acatgggcca cgcccgggca accgacatcg cggcccttat caggaaggta aaggaggagg
      721 tcttctcgaa gctcggggtc acgctcgagg aagaggtgga actctggggg ttcaatggat
      781 agaacgggtc tcaagaagct gaagataggc gtcctcctgg gaggaaggtc gtcggaacgc
      841 gaaatatccc tgaagagcgg cgcggccgtc cttcaaagcc ttgtcaggtc gggatacgac
      901 gcggtggcca tcgacgccag ggaccgcctt gtcgaaaaac tgaaaaagca gaaggtcgac
      961 gcggccttca tcgtcctcca cggcagatgg ggagaggacg gcacggtcca gggcctcctc
     1021 gagatcatgg gaatacccta cacggggccc ggcgtcctcg gctcgtcggc tgcgatggac
     1081 aaggccgtca tgaaattcat cctcgaagcg acggggatcc cgacaccggc ctacgcaata
     1141 gcgggagcgg gcgaaaggat caccatgaag ccccccttcg tggtgaaacc cgccaatgag
     1201 ggatcgacga tcggcatatc gatggtccac aggaggaagg acaccgccgc ggccatggaa
     1261 ctcgccctca gatacgacag gaagatcgtt atcgaggagt tcgtgcccgg gcaggagatc
     1321 accgtggccg tcgtcaacgg aaaggtcctc cccgttgtgg aggtcaggcc gctgtcgggc
     1381 ttttacgatt tcgaatcgaa gtacacgaag gggaagacgg aatacatcgt ccccgcggag
     1441 ataccgcgga aggtggccaa gaaggctgag gccatcgcca tggacgtcta ccgcaggttc
     1501 gacctctccg gatgcgtgcg gacggacatg ctcgtaaggg acgggatgcc ccttgtcatc
     1561 gacatcaaca cgtccccggg catgacggag acatccctcg tccccaaggc ctgggcctgt
     1621 cagggaggct cgttcgatga gctcatcgag gagatacttg gaggggcgtc gctgaagaca
     1681 tgaagaagct tctttacata ctcttcatcg ggccggtgtg catcctttcg atgctctcca
     1741 tcgcctacct gttctcccgg gaggagcccc tgttcttcct gcagaacata aaggtgaacg
     1801 gcatctccca gctcggcgac aaggagacga tggccaagat cgcaccccat ctcacggaga
     1861 gcatcttcaa ggtggatgtg gcaaaggtcc gggacgccct cacatcccat cctttcgtaa
     1921 aggaagtgag cgtgaagagg gtctatccct tctccatcgt catcgacgtg aaggagaaga
     1981 taccgtcggc cctctgggtg gcgggcgacg gcaccgtcca tatcctcgac gaaaagggcg
     2041 agccctaccg tggcctcggg aaggacgagg taaagggcct ctatcttata agcacccggg
     2101 agagatcgga tgcgaagaag gtcttcagcg agacgaacgt cttcactgtc ggcacaa
//
LOCUS       DAOQTR010000407         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_64523_length_2151_cov_2.118798, whole genome shotgun sequence.
ACCESSION   DAOQTR010000407 DAOQTR010000000
VERSION     DAOQTR010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64523_length_2151_cov_2.118798"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKN85_09210"
     CDS             complement(<1..191)
                     /locus_tag="PKN85_09210"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNT44629.1"
                     /translation="MDRKRNISLVVEYDGTNYHGWQCQPNGVTVEETLKGAIERILDH
                     PVKMYSAGRTDAGVHAFGQV"
     gene            complement(252..578)
                     /locus_tag="PKN85_09215"
     CDS             complement(252..578)
                     /locus_tag="PKN85_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44630.1"
                     /translation="MSMKYLAIIAAVVSLALILPSCSKEEKSLKEELRVMKEENNFLK
                     AENIALKKEVEELYRRLEERDKARVVPETQVKPEALKKDTKPAAAERPERPGAEKRKP
                     ETETPR"
     gene            725..>2151
                     /locus_tag="PKN85_09220"
     CDS             725..>2151
                     /locus_tag="PKN85_09220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HNT44631.1"
                     /translation="MQSSVVTIPIEEEMKRSYLDYAMSTIIGRALPDIRDGLKPVHRR
                     ILYAMYELNNTHDKPYKKSARVVGDVIGKFHPHGDQAVYDAITRMVQDFSLRYPLVDG
                     QGNFGSVDGDAPAAMRYTEIRLSRVAEEILRDIEKDTVLFRPNYDDSLVEPMVLPSRL
                     PNLLINGSSGIAVGMATNIPPHNLKEVINGLVAFIENRDIPLSELMEIIPGPDFPTQG
                     IIYGRQGIVEAYQSGRGHIVLRAKADIEKRKKDGREAIVITEIPYQVNKARLIENIVE
                     LINAKRIEGISNIKDESSREGMRIVIDLKKGEIPEPILNKLYKHTQLQTTFGIIFLTI
                     VNNEPKLLNLKEMLHEFVEFRKEIVTKRSTFELNKALDRLHLLEGLKTALDHIDEVIA
                     IIKGSANTPEAREALIARFGFSEKQATNILEMRLQRLTSLERTKILDDHKNTSDEIDR
                     LRRVLADESLLIDIVKTELIEMRDK"
BASE COUNT          490 a          666 c          548 g          447 t
ORIGIN      
        1 acctgaccga aggcatgcac ccccgcgtcg gtcctcccgg cactgtacat cttgacgggg
       61 tggtcgagta tcctctcaat tgccccttta agcgtctctt cgacggtaac gccgttgggc
      121 tggcactgcc agccgtgata gttcgtcccg tcgtattcca cgacaaggga tatgttcctc
      181 ttgcggtcca tcactgtttt ttgaggataa ttcctgcaaa tgtgcggttc acaacacccg
      241 aaggtcccgg atcatcgcgg tgtctcggtc tccggcttcc tcttctccgc gcccggcctt
      301 tcaggcctct ccgccgcggc aggtttcgtg tcctttttca gcgcctcggg cttcacctgt
      361 gtctcgggta ccaccctggc cttgtccctc tcctcgagtc tcctgtaaag ttcctcgacc
      421 tctttcttga gagctatatt ctcggctttc aggaagttgt tctcctcttt catgaccctc
      481 aattcctcct tgaggctctt ctcctccttg ctgcaggaag gcaggatgag ggcaagggac
      541 accacggcgg caatgatggc aaggtatttc attgacatta ttcttccacg acggtccggc
      601 ggttgtcaac tgcttcctgt aaatgttcct cgcgtgcggg catcctgccg tccctttcat
      661 ttacacccct cgcggaatat ggtacattaa agcaaaaatt tacgaagtat cccggaggat
      721 cacaatgcag tcatctgtcg ttaccatccc gatagaagag gagatgaagc gttcatacct
      781 cgattatgcc atgagcacca taatcggccg tgcgctgccc gatatcaggg atgggctgaa
      841 acctgttcac agacgaatac tgtacgccat gtacgagctt aacaacaccc acgacaagcc
      901 ctacaagaag tcggcgaggg tcgtcggtga cgtcataggt aagttccatc cccatgggga
      961 ccaggcggtc tacgacgcca tcaccaggat ggtccaggat ttctctctcc gctatcccct
     1021 ggtggacggt cagggcaatt tcggctccgt cgacggtgac gcccccgcgg ccatgcggta
     1081 cacggagata cgcctgtccc gcgtcgccga ggagatcctc agggacatag aaaaggacac
     1141 cgtccttttc aggcccaact acgacgactc gctcgtggag cccatggtcc ttccgtcccg
     1201 cctgcccaac ctcctcatca acggctcttc gggtattgcc gtcggcatgg cgacgaacat
     1261 cccgccccac aacctgaagg aggtcatcaa cgggcttgtc gccttcatcg agaacaggga
     1321 catcccgctg tccgagctca tggagataat cccgggaccc gacttcccga cgcagggcat
     1381 catctacgga aggcagggga tagtcgaggc ctaccagtcc ggcaggggac acatagtcct
     1441 gcgcgccaag gcagacatcg aaaaaagaaa gaaggacggc agagaggcca tcgtcatcac
     1501 cgagatacct taccaggtga acaaggcccg cctgatcgaa aacatcgtcg agctcatcaa
     1561 cgcgaagagg atcgagggca tatcgaacat caaggacgaa tcgagcaggg agggaatgcg
     1621 catagtcatc gacctcaaga agggggagat acccgaaccc atcctcaaca agctctacaa
     1681 gcacacccag ctccagacga ccttcggcat catcttcctc accatcgtca acaacgaacc
     1741 aaaactgctg aacctgaagg aaatgctcca cgagttcgtc gagttccgca aggagatcgt
     1801 cacgaagagg tcgaccttcg agctcaacaa ggctctggac aggctccatc tcctcgaagg
     1861 gctcaagacc gccctcgatc acatcgacga ggtgatcgcc atcatcaagg gttccgccaa
     1921 cacacccgag gcccgcgagg ccctcatcgc ccgttttggc ttttccgaga aacaggcgac
     1981 aaacatcctc gagatgcggc tccagcgatt gacctccctg gaaaggacga agatactcga
     2041 cgatcacaag aacacgtccg acgagatcga ccgtttaagg cgggtactgg ccgacgagtc
     2101 tctcctcatc gacatcgtga agacggaact tatcgagatg cgcgacaagt a
//
LOCUS       DAOQTR010000408         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_64581_length_2149_cov_4.933142, whole genome shotgun sequence.
ACCESSION   DAOQTR010000408 DAOQTR010000000
VERSION     DAOQTR010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64581_length_2149_cov_4.933142"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..740
                     /locus_tag="PKN85_09225"
     CDS             93..740
                     /locus_tag="PKN85_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT44632.1"
                     /translation="MRICRDKLSRSGGFTLLELVVSIALVAMIVVILAASMRAGHRSL
                     EGGERKAEWMERLRVSMRLIDSQLQSCLPLTVKEQKDLKRSVFIGAHDSMTFASNRSL
                     FGGRKGYTIASYRIEKDEKGKLALYLKETTIGMKNVKETKLLVGFDDIRFEYLQSAGI
                     KGLGGGDWSDELADTTHLPQKIKLHLEKGARKFSLTIPLRTRKPPGNDPDEESGT"
     gene            737..1714
                     /locus_tag="PKN85_09230"
     CDS             737..1714
                     /locus_tag="PKN85_09230"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
                     /protein_id="HNT44633.1"
                     /translation="MRKGKHLLCKEDGFALIMVLWVIIILMVIVMSFSVLAKTESRAA
                     LFFRDSLAEGFLAQAGLERAILEIYYRQDNLNKTVVPDSGETLRVDGTAIEGDVGDGR
                     YVVRIFNEAGRINLNTMSDGNKIILDNLLVNLGVPKETADTVVDSALDWMDKDVLHRL
                     NGAEDDYYQSLPNPYRAKNGPFDTVEELLLVKGMTPDILFGTKEHKGLIHFITVYGDA
                     SKINVNFAAKEVIMALPGVSEDRAARIIDRRKQAELKTIDDVRGIMGGEYGNVSSYID
                     VAESVGYAIESTGYREGAGEGHGIRATLLVRTGGTYEYIYYKIPAETTK"
     gene            1726..>2149
                     /locus_tag="PKN85_09235"
     CDS             1726..>2149
                     /locus_tag="PKN85_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44634.1"
                     /translation="MALSPAIEKGIAYVRTVREKVEPLCLRIWAALNCNLVEGIILPE
                     RFLAVCMERGCVSAAAGERFLNRRRLIGSGRYALEGDGFPAPDDLAAAASRAMKELGR
                     RGSKVLLGVPREWVVIRTADMPSTVKENMGPVVTYEIDR"
BASE COUNT          529 a          555 c          648 g          417 t
ORIGIN      
        1 ggtggtgctc aatgtctcct ggcagcaggg ggtgaggaaa aggtcattct ctctgaagac
       61 cttcaaaaca gtgaggagag aggtgatcaa aaatgagaat atgtagggac aagctttcgc
      121 ggtcaggggg ttttactctc ctggaactcg tggtctccat cgcacttgtc gccatgatcg
      181 tagttattct cgccgcctcg atgcgtgccg ggcaccgctc cctcgagggg ggcgagagaa
      241 aggcggagtg gatggagcgc ctcagggttt ccatgagact catcgattcc cagttgcaat
      301 cctgcctccc cctcaccgtg aaggaacaaa aggacctcaa gagatcggtg ttcataggtg
      361 cccacgattc gatgacattc gcatccaacc gttctctctt tggcggccgg aaggggtata
      421 ccatagcctc atacaggatc gaaaaggatg aaaagggaaa gctggcgctc tatctgaagg
      481 agaccaccat aggcatgaag aacgtgaagg aaacgaaact cctcgtcggt ttcgacgaca
      541 tccggtttga ataccttcaa tccgcgggga taaaggggct gggagggggc gactggtcgg
      601 acgaactggc ggacaccacc catctcccgc aaaagataaa gctccacctg gaaaaaggcg
      661 ccaggaagtt ctctctcacg atacccctgc gaaccaggaa gcctcccggg aatgaccctg
      721 atgaagagag cgggacatga gaaaagggaa acacttactt tgcaaagaag atggctttgc
      781 cctcatcatg gtgctgtggg tgatcattat cctcatggtc atcgtcatgt ccttttccgt
      841 gcttgcgaag accgaaagca gggcggccct gttctttcga gattcactgg cagaaggttt
      901 tctcgcgcag gcggggctgg agcgggcgat cctggagata tactaccggc aggacaacct
      961 gaacaagacc gtcgtccccg acagcggaga gaccttgagg gtcgacggca cggctatcga
     1021 gggcgatgtc ggtgatggca ggtatgtcgt caggatcttc aacgaggcgg ggcggatcaa
     1081 tctcaatacc atgagcgatg ggaacaagat catcctcgac aatcttctcg tcaacctcgg
     1141 agttccgaag gagacggcgg acaccgtcgt ggattcggct ctcgactgga tggataagga
     1201 cgtcctccac cgtctgaacg gcgccgaaga cgactactac cagtccctcc ccaaccctta
     1261 cagggcgaag aacgggccct ttgatacggt ggaggaactc cttctggtaa aaggtatgac
     1321 tcccgacatc ctcttcggaa caaaagagca taaagggctc atccacttca tcaccgtcta
     1381 tggggatgcg tcaaagataa atgtgaattt tgcagcgaaa gaggtcatca tggcactgcc
     1441 cggcgtgtcg gaggaccggg cggcaaggat catcgatcga aggaaacagg cggagctgaa
     1501 aaccatcgat gatgttcgcg gcatcatggg aggggaatac ggcaacgtat catcttacat
     1561 agacgtcgct gaatccgtcg gctacgccat cgaatcgaca ggttacagag agggcgccgg
     1621 ggaaggccac ggtatccggg ccacactcct cgtcaggacg ggagggacat acgagtacat
     1681 ctattacaag atccccgcgg agacaacgaa atgagaattg gatccttggc tctgagcccc
     1741 gccattgaga aaggcatcgc atacgtgagg accgtgaggg aaaaagttga acccctgtgc
     1801 ctgagaatat gggctgcgct gaactgcaac ctcgttgaag gcatcatcct gcccgagcgc
     1861 ttcctggcgg tctgcatgga acggggatgc gtgtcagcgg cagcgggtga gcgctttctc
     1921 aaccgcaggc ggctcatagg atctggaagg tacgcccttg aaggagatgg atttccggcc
     1981 cccgacgatc ttgccgccgc cgcgtcccgc gcaatgaagg aactggggcg cagaggctcg
     2041 aaggtgctcc tgggtgttcc cagggagtgg gttgtcataa ggactgcgga catgccctcg
     2101 accgtaaaag agaacatggg acctgtagtg acttacgaga tcgacaggc
//
LOCUS       DAOQTR010000409         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_64784_length_2145_cov_3.283732, whole genome shotgun sequence.
ACCESSION   DAOQTR010000409 DAOQTR010000000
VERSION     DAOQTR010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64784_length_2145_cov_3.283732"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..2087)
                     /locus_tag="PKN85_09240"
     CDS             complement(171..2087)
                     /locus_tag="PKN85_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT44635.1"
                     /translation="MGLVRIHDVSLSFGGPRLLDCATIQIESGERIGLLGRNGSGKST
                     FMKLIAGEVHPDSGEVVYGGDVRVALLPQDVPDDLPGTVYDVVASGGFEHARLLSKYH
                     DLTMGIARGEDDGLLKTLEQVQHLIEASGAWHYHQRINAVIERTALDENAPFRTLSAG
                     TKRRVFLAKALVADPDLLLLDEPTNHLDVATILWLEEFLLRYGKTVIFVTHDRAFLKR
                     LATRIVEIDRGRLTSYTCDYPAYLERRQALLEAQEKQWSEFDKKLAREEVWIRQGVKA
                     RRTRDEGRVRALMRMREERAARQEQTGLSRFSIEEAERSGRMVVDARGVFFAWEDRKI
                     IDGFSATIIRGDRVGIIGPNGCGKTTLLRILLGQLVPDEGKVRLGTNVAVAHFDQLRS
                     QLDETRTLRENIGGGNDMIITGSGSRHVVGYLQDFLFSPERTMSPVGSLSGGERNRLL
                     LAKLFIVPSNVLVLDEPTNDLDTETLELLEEKLLEYNGTILMVSHDRAFLNNVVTSTI
                     VFEEDGGLREYVGGYDDWLRQANSALAAERTVPKEQKQKKAKAPQEKKRLSFKEAREL
                     ESLPPLIEALEEEKRQLTETLNSPEFYAGRDLERIRTVNDRWGALEKELNAAYHRWDE
                     LEELKAALAGPSER"
BASE COUNT          377 a          666 c          633 g          469 t
ORIGIN      
        1 cctgtagacg agtcgaaccg gccggcggcc acgcgtgtat ttggcccccg cctttcccgc
       61 ggcatgctct tcaagacgtt ttctaagatt gttcgtccag ccgcaataga gggttttgtc
      121 ggaacattcg aggatgtaga cgaatatcgc agcgctgtct gtcacggcgg tcatcgctcc
      181 gatgggccgg cgagggctgc cttcagttct tccagttcgt cccagcgatg ataggcggca
      241 ttgagttcct tctcgagggc accccagcgg tcattgacgg tccttatcct ttcgaggtcg
      301 cgtcccgcat agaactcggg tgaattgagg gtctccgtca attgccgctt ttcctcctcc
      361 agggcctcga tgagtggcgg cagggattcg agctcccggg cctctttgaa ggacaaccgc
      421 ttcttttcct gaggcgcctt cgccttcttc tgtttttgct ccttcggcac cgtccgctcc
      481 gccgccaggg cagagtttgc ctgcctgagc cagtcatcgt agccgccgac atattccctc
      541 aagcccccat cctcttcgaa gacgatcgtc gaggtcacca cattgttcag gaatgccctg
      601 tcatggctca ccatcaggat ggtgccgttg tattcgagga gtttttcttc gaggagctcc
      661 agcgtttccg tgtcgaggtc gttcgtaggc tcgtcgagga cgaggacatt ggaagggacg
      721 atgaagagct tcgcgagaag caggcggttg cgctcgccgc cggaaaggga gccgacaggc
      781 gacattgtcc gctcgggaga aaagagaaag tcctggaggt accccaccac gtgccgggag
      841 ccgctcccgg tgatgatcat gtcgttgccg ccgccgatgt tctctctcag cgttctcgtc
      901 tcatcgagct gggagcgcag ctggtcgaag tgcgcaacgg ccacgttcgt ccccagcctg
      961 acctttccct catctggcac gagctgaccg aggagtatcc tgagaagggt tgtcttgccg
     1021 cacccgttgg ggcctatgat ccccacccga tccccccgga tgatggtggc ggagaagccg
     1081 tcgatgatct tcctgtcctc ccaggcaaaa aagacgcccc ttgcatccac aaccatcctg
     1141 ccgctgcgtt ccgcctcctc gatggagaac cgggacaggc ccgtttgctc ctgccgcgcc
     1201 gcccgttcct cccgcatccg catgagcgca cggaccctgc cttcatcacg ggtgcggcgg
     1261 gccttgacgc cctgtctgat ccacacctct tcccgggcca gcttcttgtc gaattcggac
     1321 cattgtttct cctgggcttc aagaagggcc tgcctgcgtt cgagatatgc ggggtaatcg
     1381 caggtgtacg atgtcaggcg tccgcggtcg atctctacta tcctcgtcgc caggcgcttg
     1441 agaaacgccc ggtcgtgggt cacgaagatt acggtctttc catatctcag gaggaactcc
     1501 tcgagccaga gtatggtggc cacatcgagg tggttcgtcg gttcgtcaag gaggagaagg
     1561 tccgggtccg cgacgagggc ttttgccagg aacacacgcc gcttcgtgcc cgccgaaagt
     1621 gtcctgaaag gcgcgttctc atcgagcgcg gtccgttcga tcaccgcatt gatcctctgg
     1681 tggtaatgcc aggccccgga tgcttcaatg aggtgctgca cctgttcgag cgtttttaag
     1741 agcccgtcgt cctcaccgcg ggcgatgccc atcgtgaggt cgtggtattt gctgagaagc
     1801 cgggcgtgct cgaacccgcc cgaggcgacg acgtcataga cggtcccggg gaggtcgtcg
     1861 ggaacatcct ggggaaggag cgcgacgcgg acatcgccgc cgtagacgac ctctcccgaa
     1921 tcgggatgga cctcgccggc gatgagcttc atgaaggtcg acttgcccga cccgttgcgc
     1981 cccagaagac ctatcctttc accagattcg atctgaatgg tggcgcagtc gaggagccgc
     2041 ggcccgccga agctcaagga aacatcatgt atgcgcacaa gtcccatgtc gctctcaagg
     2101 tcttcagtat atcacgacgg aagattcagg tataccggcc cctac
//
LOCUS       DAOQTR010000410         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_64827_length_2144_cov_3.619435, whole genome shotgun sequence.
ACCESSION   DAOQTR010000410 DAOQTR010000000
VERSION     DAOQTR010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64827_length_2144_cov_3.619435"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PKN85_09245"
     CDS             complement(<1..435)
                     /locus_tag="PKN85_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNT44636.1"
                     /translation="MVKLYNIVESRVTECVGAAGNIAVYINPDENERRRLIDQDQIDE
                     HTLQSALDPDELSRIEIESNHVAIILKVPVNYQAEHAFEFLVSSMGMFLFENKLVIVM
                     AEDLPIFDSKTFARVSRLAETMLKIVNYSIFRFLEHLRVIDMV"
     gene            634..867
                     /locus_tag="PKN85_09250"
     CDS             634..867
                     /locus_tag="PKN85_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44637.1"
                     /translation="MKKLLLASTLLVLIAAVVIVSPPAASASSCREDCLSDCCTDSRC
                     TKEKEARCYEDCLRDCNSAATKLKKDKSTLPPT"
     gene            complement(951..>2144)
                     /gene="eno"
                     /locus_tag="PKN85_09255"
     CDS             complement(951..>2144)
                     /gene="eno"
                     /locus_tag="PKN85_09255"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNT44638.1"
                     /translation="AMGRAMVPSGASTGEREALELRDGDAKRYKGKGVKKAVENVNNI
                     IAPEVEGLDALDQAYIDKVLVDADGTENKSSLGANATLGVSMAVTRAAADFLGVELYQ
                     YIGGIRGREIPVPMMNVINGGAHAQNSLDVQEFMIVPAGAPSFSEALRMGAEVFHTLK
                     GILKDKGYSTGVGDEGGFAPQIESTTEALDTLMTAIAKAGFKAGKDIFLALDVAASEL
                     YKKGKYHIDGNVWDAGKLVDFYEAIVKKYPIVSIEDGFAQNDWKGWKKFTDRCASTIQ
                     IVGDDIFVTNPGIFAEGIGKGIANSILIKLNQIGTVTETLTTIEMAKRAGYTCVISHR
                     SGETEDTFIADLAVATNVGQIKTGSASRSERIAKYNQLLRIEDELGELAIFGGTGVFY
                     SVRKK"
BASE COUNT          423 a          659 c          579 g          483 t
ORIGIN      
        1 gaccatgtcg atgaccctca ggtgctccag gaagcggaag atgctgtagt tgacgatctt
       61 cagcatcgtc tcggcaagac ggctgacacg ggcaaaggtc ttgctgtcga agatgggcag
      121 gtcttccgcc atgactatga cgagcttgtt ctcgaagagg aacatgccca tggatgaaac
      181 gaggaactcg aaggcatgct cggcctgata attgacaggc accttgagga tgatggcgac
      241 gtgattcgat tcgatctcga tacgggaaag ctcatcgggg tcaagtgccg actgcagggt
      301 gtgctcgtct atctggtcct ggtcgatgag acggcgtctc tcgttctcat cggggttgat
      361 gtagacggct atgtttcccg cggcgccgac gcattccgtt acccgtgatt ccactatgtt
      421 gtagagcttt accatacgac ataccgttca tgtttggatt gatacgtaca atgtatccca
      481 tcttctcgga aaaagcaccg aaattctcaa caaaagatgc ctcctgtcca tcgacgtcgc
      541 ctccccatcc cgcctcaagt gtgacacagg acattgactg tcaaaaacca tagtgttata
      601 aatcgtcatg ccccatatca aggaggtcac acgatgaaaa aacttcttct cgcatcaacc
      661 ctgctggtcc tcattgccgc cgtggtcatc gtatcgccgc ccgcggcgag cgcgtcgtca
      721 tgtcgcgagg actgcctctc cgattgctgc actgattccc gatgcaccaa ggaaaaagag
      781 gccaggtgct acgaggattg cctgagggac tgcaacagcg cggccacgaa actcaagaaa
      841 gacaaatcga cactgcctcc cacgtaacgg aaaaggacca gcgccccctt cacaggcggg
      901 cgccggtccc tgatattcct gccgccgata gaacccttat cctggaaccc ttacttcttc
      961 ctgacgctgt aaaaaacgcc cgtgccgccg aatatggcca gctcgccaag ttcatcctcg
     1021 atcctgagaa gctgattgta cttcgcaatc ctctcgcttc tcgaggcgga ccccgtcttg
     1081 atctggccca cgttggtcgc aaccgcaagg tcggctatga aggtgtcctc ggtctcgccg
     1141 gaccggtgtg agatgacaca ggtgtaaccc gcccgcttgg ccatctcgat ggtcgtcaat
     1201 gtttccgtga cggtgccgat ctgattgagc ttgataagga tgctgttggc tatccccttc
     1261 ccgatcccct cggcgaagat ccccgggttc gtcacgaaga tgtcgtcccc gacgatctga
     1321 atggtggagg cacaccggtc ggtgaatttc ttccagcctt tccagtcatt ctgggcgaac
     1381 ccgtcctcta tggagacaat ggggtacttc ttcacgatcg cctcgtagaa atccaccagc
     1441 ttgcccgcgt cccacacgtt gccgtctatg tggtattttc ccttcttgta aagctcgctc
     1501 gcggcgacat cgagggccag gaagatgtcc ttccccgcct tgaaaccggc cttggctatg
     1561 gccgtcatga gcgtgtcgag cgcctccgtg gtcgattcta tctgcggggc gaagccgcct
     1621 tcgtcgccga cgcccgtcga gtatcccttg tccttcagga tacccttaag ggtgtggaac
     1681 acctccgcgc ccatcctcag ggcttcgctg aatgacggcg cgccggcggg gacgatcatg
     1741 aactcctgca catcgaggga gttctgggca tgggcgccgc cgttgatgac gttcatcatg
     1801 ggaacaggta tctcccgccc cctgatcccc ccgatgtact ggtacagctc aacccccaga
     1861 aagtcggccg cggcccgcgt gacggccatc gaaacgccca gggtggcgtt ggcaccgagg
     1921 ctgctcttgt tctcggtacc gtcggcatcg acaaggacct tgtcgatgta ggcctggtcg
     1981 agggcatcga gtccctcgac ttccggggcg atgatgttgt tcacgttctc gacggccttc
     2041 ttcacgccct tgcccttgta cctcttcgca tcgccgtcgc gcagctcaag ggcttccctt
     2101 tcacccgtgg aggcacccga gggtaccatc gcgcgcccca tcgc
//
LOCUS       DAOQTR010000411         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_64841_length_2144_cov_2.747726, whole genome shotgun sequence.
ACCESSION   DAOQTR010000411 DAOQTR010000000
VERSION     DAOQTR010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64841_length_2144_cov_2.747726"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PKN85_09260"
     CDS             complement(<1..1063)
                     /locus_tag="PKN85_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNT44639.1"
                     /translation="MGMTITEKILAAHADRDRVEPGEILDCRIDLAMANDITAPLSLE
                     EFEKVGAKAVFDPNKVVFVLDHFTPNKDILSAENCKKIREFSRKYGIVNFFEGGECGI
                     EHALLPEKGLVVAGDLVIGADSHTCTYGALGAFSTGMGSTDITYGMVTGRIWLKVPES
                     IKFHCHGKWQQWVTGKELILHILGMIGVDGALYSAMEYSGEAIAALSMDSRFSISNMA
                     IEAGGKNGIFPVDDKTLAYMKEHATRPYKVYASDADATYSEVYEIDVSKVGFSVACPS
                     LPSNVKDAGELGSIDVDQVVIGSCTNGRLEDLEMAAAILTGKRVSKGLRCIVIPATPA
                     IYGEAMRRGYFQTFLDAGCI"
     gene            complement(1075..>2144)
                     /locus_tag="PKN85_09265"
     CDS             complement(1075..>2144)
                     /locus_tag="PKN85_09265"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNT44640.1"
                     /translation="CKVVAEVIDVGAITVNISDTVGYAVPHEFGELIAYIRKNVPNID
                     KAIISVHCHNDLGLATANSIAAIHNGARQVECTINGIGERAGNASLEEIAMTLRTRKT
                     SFTADTRIVSEKIYPTSRLITSITGVPVQPNKAIVGANAFAHESGIHQDGLLKAKLTY
                     EIMTPESVGISKSSLILGKHSGRHAFRDRIENLGYILDDKELNLAFKRFKALSDMKKN
                     VYDEDIEMIIMDEIYKTAERAKLVYLNVSCGNTTIPTATVKMEIDGNIFQDVGTGDGP
                     VHAAFKVIKRLLKTTSKLEKFSVNSITQDMDAQGEVFVKLSEKGLKAIGKGADTDIIV
                     ASAKAYINALNRLEYMKTKKS"
BASE COUNT          351 a          627 c          639 g          527 t
ORIGIN      
        1 tgatgcagcc ggcatccagg aatgtctgga agtatccccg gcgcattgcc tcgccgtaga
       61 tcgcgggggt cgcggggatc acgatgcatc tcaaaccttt gctgaccctc ttccctgtga
      121 ggatggcggc cgccatctcc aggtcctcga gtctgccgtt ggtgcaggaa ccgatgacga
      181 cctggtccac atctatgctg cccaactccc ccgcgtcctt cacgttggac gggagcgagg
      241 ggcacgcgac gctgaagccg accttcgaaa catcgatctc gtacacctcg ctgtatgtcg
      301 cgtcggcatc gctcgcgtag accttgtagg gccgcgtggc gtgctccttc atgtaggcca
      361 gcgtcttgtc gtcgacggga aatatgccgt tcttcccccc ggcctcgatg gccatgttgg
      421 agatggagaa ccgcgaatcc atggacaggg cggctatggc ctcaccgctg tattccatgg
      481 cgctgtaaag cgcgccgtca acccctatca ttcccaggat atggaggatg agttccttgc
      541 ccgtgaccca ctgctgccat ttcccgtggc agtggaactt gatgctctcg gggaccttca
      601 gccagatgcg tcccgtcacc atgccgtagg tgatatccgt cgagcccatg cccgtggaaa
      661 aagcgccaag ggccccgtag gtgcaggtgt ggctgtcggc gccgatgacg aggtcgcccg
      721 ccacaacgag tcctttttcg ggcaggaggg catgttcaat gccgcactcg ccgccctcga
      781 agaagttgac gatgccgtat ttccgggaga attcccgtat cttcttgcag ttctcggctg
      841 acaggatgtc cttgttgggt gtgaaatggt caaggacgaa gacgaccttg ttggggtcga
      901 agacggcctt tgccccgacc ttctcgaatt cctcgaggga caggggcgcc gtgatgtcgt
      961 tcgccatggc gaggtctatc ctgcaatcca ggatctcacc gggttcgacc cggtccctgt
     1021 ccgcgtgcgc tgccagtatc ttttccgtaa tggtcattcc catatcgttt cctctcagga
     1081 ttttttcgtt ttcatgtatt ccagcctgtt cagcgcgttg atgtacgcct tggcgctggc
     1141 gacgatgatg tccgtgtccg ctccttttcc tatggccttg aggcccttct cggagagctt
     1201 gacgaagacc tctccctggg cgtccatgtc ctgggtgatg gagttcacgg agaacttctc
     1261 gagcttgctc gtcgtcttga ggagcctctt gatgaccttg aaggccgcgt ggacgggacc
     1321 gtcgccggtt cccacgtcct ggaagatgtt tccgtctatc tccatcttca ccgtagccgt
     1381 cggtatcgtc gtgttcccgc agctcacgtt gaggtacacg agcttcgccc tctcggccgt
     1441 cttgtatatc tcgtccatga tgatcatctc gatgtcttcg tcgtaaacgt tcttcttcat
     1501 gtccgagagc gccttgaagc gcttgaaggc caggttgagc tccttgtcgt cgaggatgta
     1561 ccccaggttt tcgatacggt ccctgaaggc gtgcctgccg gagtgcttcc cgaggatgag
     1621 ggagctcttg gaaatgccca ccgattctgg ggtcatgatc tcgtaggtga gctttgcctt
     1681 caggaggccg tcctgatgga tacccgactc gtgagcgaag gcgttggcgc cgacgatggc
     1741 cttgttgggc tgcacgggca cgcccgtgat ggaggttata agcctgctcg tcgggtatat
     1801 cttctcggag acgatgcggg tgtcagcggt gaagctcgtc ttcctcgttc tcaaggtcat
     1861 ggctatctct tcgagagagg cgttccccgc cctttcaccg atgccgttta tcgtgcattc
     1921 cacctggcgc gcgccgttgt gaatggcagc gatggagttg gccgtggcaa ggccgaggtc
     1981 attgtggcaa tggacgctga tgatggcctt gtctatgttg gggacgttct tcctgatgta
     2041 ggcgatgagc tccccgaact cgtgggggac ggcatagccg acggtgtccg atatgttgac
     2101 cgtgatcgcc ccgacgtcga tgacctcggc gaccaccttg caca
//
LOCUS       DAOQTR010000412         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_65663_length_2127_cov_1.906853, whole genome shotgun sequence.
ACCESSION   DAOQTR010000412 DAOQTR010000000
VERSION     DAOQTR010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65663_length_2127_cov_1.906853"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1566)
                     /locus_tag="PKN85_09270"
     CDS             complement(124..1566)
                     /locus_tag="PKN85_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNT44641.1"
                     /translation="MMDFPPVFVDHVGGRLIIGLIACIHVLINHPLAVGAYPLLTWME
                     WWAAKNNRPDIDRVAYRITFIVFIITTTVGAMTGVGIWLSTSLFAPFAIGSLLRVFFW
                     GWFLEWLVFISEVVLIMWWFLSWKKANTPEKKRKHIRIGVALSIMSWLTMALIVAVLG
                     FMMKPGEWNQTKAFWDAMTNPLYLPQLGFRTFFALMTGAAFVWFAAFFFTGKKEPGDP
                     LRVREWLVYRMPHVVLVSMLGTYLFGLIYWNAVPETMKANSSVALLTQQFMNWHDQFK
                     VVLGWTLITFLLIAAGGITGMYRVPRLALLIPTFMGIWLLGHFERAREFMRKPWVIGE
                     YMYSNGVHKDELAFLQSEGLLKHATYVSHREVTDANRTEVGRDIFMLACTRCHSTTGL
                     NGVVEKFEIMYGPDKAWDPGAMESFILSMHQARPYMPPFPGNQAEAAALVDYLLELKE
                     TRTSLPGAQSAGVVVAPLPAPVPATAISKN"
     gene            complement(1635..>2127)
                     /locus_tag="PKN85_09275"
     CDS             complement(1635..>2127)
                     /locus_tag="PKN85_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44642.1"
                     /translation="RETLAYALKRRGCEILQAGEGAEALAISPGGGDLDGILLDMRLG
                     SEDGMSLIPELKRRFPLSRLIVLTGYGSIPGAMEAVRLGADDYLLKPVGVDRIVAAFS
                     GGEKIAPLSFVEDLPSLARVEWEHIQRVLSDCGGNVSHAAEALGIDRRTLQRKLSKLP
                     PLS"
BASE COUNT          488 a          610 c          647 g          382 t
ORIGIN      
        1 acgaggaaga agacaacaag cagcgctgcg atgaccgcct cgggaagagg cagcggcaat
       61 ggcagatcgc ggggaacagg gagagtggca acaaaagtga tcattgaaat caaaaggaca
      121 aagctagttt ttcgaaatgg ccgtggcggg gaccggcgcg ggtagtggag caaccaccac
      181 cccggcgctc tgggcaccag gaagggatgt gcgggtttcc ttgagttcga gcagatagtc
      241 gacaagagct gcggcctcgg cctgatttcc ggggaagggc ggcatgtagg gccgggcctg
      301 gtgcatgctg aggatgaaag actccatcgc cccggggtcc cacgccttgt cgggtccata
      361 catgatctcg aacttttcca cgacgccatt gagccccgtt gtgctatggc agcgggtaca
      421 ggcgagcatg aaaatgtcgc gtccgacctc ggtccggttg gcgtccgtca cctcgcgatg
      481 gctgacatag gtggcgtgct tgagcagtcc ctcggactgg aggaaggcaa gctcatcctt
      541 gtggactccg ttcgagtaca tgtattcccc aatcacccag ggtttgcgca tgaattcgcg
      601 ggctcgctcg aagtgcccga gtagccagat ccccatgaag gtggggatga gcagggcgag
      661 acgtggcacc cggtacatcc cggtgatgcc tccggccgcg atcaaaagaa aggtgatcaa
      721 agtccagccg agaacgacct tgaactgatc gtgccagttc atgaactgct gggtgagcag
      781 ggcaaccgag ctgttcgcct tcatcgtttc gggcacggcg ttccagtaga tcagaccgaa
      841 gaggtaagtg cccagcatgc tcactaggac aacatggggc atgcggtaga cgagccactc
      901 acggacgcgg agagggtcgc ccggctcttt tttcccggtg aagaagaatg cggcgaacca
      961 cacaaaagcc gccccggtca tcagcgcaaa aaaggtccga aatcccaact gcgggagata
     1021 gagcggattg gtcattgcat cccagaaggc cttcgtctgg ttccattccc ccggcttcat
     1081 catgaatccg agcacggcta cgatgagtgc catcgtgagc cacgacatga tgctgagagc
     1141 cacgccgatc cggatgtgtt tgcgtttttt ctcaggggtg ttcgccttct tccagctcag
     1201 gaaccaccac atgatgagca ccacctccga gatgaagaca agccactcga ggaaccagcc
     1261 ccagaagaaa acccgcagga gactgccgat cgcaaagggt gcaaagagcg aggtcgaaag
     1321 ccagatcccg actccggtca tggctccgac cgtcgtcgtg atgatgaaga cgatgaaggt
     1381 gatgcggtag gcgacgcgat caatgtcggg acggttgttt tttgcggccc accactccat
     1441 ccaggtgagg agcgggtagg cacccaccgc gagcgggtga ttgatgagga cgtggataca
     1501 ggcgatgagt ccgatgatca agcggccgcc gacgtgatcg acaaagacag gaggaaaatc
     1561 catcatggat gggaacctcc tcccgcattg ctgatctgaa aatgcggcga attgccgcac
     1621 taacagatca ctattcaact caaaggcggc aacttcgaga gcttgcgctg gagtgtgcgc
     1681 cggtctattc ccagggcctc ggccgcgtgt gagacgttgc ctccgcagtc actcaggact
     1741 cgctggatgt gctcccactc gaccctcgcc agactcggca gatcttcgac aaacgagagt
     1801 ggtgcgatct tttcaccccc ggaaaaagcc gccacgattc ggtcgacacc gaccggcttc
     1861 aacaggtaat catcggcacc gaggcgcact gcctccatcg cccccgggat gctgccgtaa
     1921 ccagtcagga cgatgagccg tgaaagtgga aaacgacgct tcagttcagg gatcagcgac
     1981 atgccgtcct cgctcccgag acgcatgtcc aggaggattc cgtcgagatc tcccccgccc
     2041 ggggaaattg ccagcgcctc cgccccctca cctgcctgga ggatctcaca accgcgtcgt
     2101 ttcagagcat aggcaagggt ctcgcga
//
LOCUS       DAOQTR010000413         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_65878_length_2122_cov_3.939042, whole genome shotgun sequence.
ACCESSION   DAOQTR010000413 DAOQTR010000000
VERSION     DAOQTR010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65878_length_2122_cov_3.939042"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2039
                     /locus_tag="PKN85_09280"
     CDS             <1..2039
                     /locus_tag="PKN85_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44643.1"
                     /translation="AYINRAVLTNRNIVAVNIYDESGRFVDSLRKNPDTMEVVDDVTP
                     SIFIIPKGAEDIHKVYYAVFHHEFIVGFFEIFYAERYINAEILARRFNLTVAFTLIGL
                     CMIVVIFVVVKKMLIAPVLTLADVSRQITKDRDYSTKLKNRSNDEIGVLYESIADMLR
                     EIERNEEELRRTRGYLGNIIESMPSMLVSVDRDGTVTQWNHATEVATGISSEHALGKN
                     LWRITDFFDSFKEAIEKIAETNEAAHLYSHRFDREETRYKDVSIYPLVADGIGGVVIR
                     MDDVTELEKKESLLQQAQKMETIGTLASGIAHDFNNILGSIIGNVSLLQYKLKSGAAI
                     PAEELSEMMATIDKASSRAADVVKQLLTLSSRRETAFSPVDLNVVVKNVLHICENTFD
                     KVVVLEARYPETKAMVWADQVQMEQIVLNLCINASHALTIMREKGGEKGGRIMVSLDK
                     VKPDRGFLSRHPDVGPEPYWLLSVEDNGVGIPEEMLPKIFDPFFTTKGAGRGTGLGLA
                     VVYNIIRQHSGFIDVVSSPGVGTIFNCFLPCLQDAAREKGAGKEQGPPRGEGVVLIVD
                     DEAGMREVAGTILQELGYSVLLAEDGDEALGVFRERKDEIDMVLLDVIMPRRSGRQVY
                     SEMKKIDPGVKVLFASGYWQDGGDEDLSSLDDAHFIQKPYTLQAIAEAVRKRMT"
BASE COUNT          497 a          579 c          636 g          410 t
ORIGIN      
        1 tcgcctacat caacagggcg gtcctgacga acaggaacat cgttgccgtc aacatttacg
       61 atgaatccgg gagattcgtg gacagcctga ggaagaaccc tgacaccatg gaggtcgtcg
      121 atgacgtcac tccgtccata ttcattatcc cgaaaggtgc cgaggacatc cacaaggtct
      181 actatgcggt ctttcaccat gagttcatcg tgggtttttt cgagatcttc tatgcagagc
      241 gctacataaa tgccgagatc cttgccaggc gcttcaacct gacggtggcc ttcacgctca
      301 tcggtctttg catgatcgtg gtcatattcg tggtcgtgaa gaagatgctt attgccccgg
      361 tcctgaccct cgcggacgtg tcgcggcaga tcacgaagga cagggattat tcgaccaaac
      421 tgaagaatag gtcgaatgac gagatcggcg tactctatga gagcatagcc gacatgctcc
      481 gcgagataga gcggaacgag gaagagctgc gcagaacgag gggctacctc ggcaacatca
      541 ttgaatccat gccttccatg cttgtttccg tcgaccgtga cggaacggtc acgcaatgga
      601 accatgccac ggaggtggcg acggggatct cgtccgaaca cgcgctggga aagaacctgt
      661 ggcggataac ggacttcttc gacagcttca aggaggcgat agagaagatc gcggagacca
      721 atgaggccgc gcatctctac agccaccggt tcgatcgcga agaaacgcgc tacaaggatg
      781 tgtcgatcta tcctctcgtg gccgacggta tcggcggcgt ggtcatcagg atggacgacg
      841 tgacggagct cgagaagaaa gagagtctcc tgcagcaggc gcagaagatg gagaccatcg
      901 gcacgctggc gagcggaata gctcatgatt tcaacaatat cctggggagc atcatcggga
      961 acgtttccct tctccagtac aagctgaaat caggcgccgc tatccccgcc gaagagcttt
     1021 cggagatgat ggcgaccatc gacaaggcaa gcagccgggc cgcggacgtt gtcaagcagc
     1081 tcctgacact ctcgagcagg agggagacgg cgttctcccc cgtcgatctc aatgttgtcg
     1141 tgaagaacgt gcttcacata tgcgagaaca ccttcgacaa ggtggtggtc ctcgaagcga
     1201 gatatcccga aacgaaggcg atggtatggg ccgaccaggt gcagatggag cagatcgtcc
     1261 tcaacctttg catcaacgcc tcccacgccc tcacgataat gagagagaaa ggcggcgaga
     1321 aaggcggcag gatcatggtg tccctcgaca aggtcaagcc cgacagggga ttcctgtccc
     1381 ggcaccccga cgtcggtcca gaaccgtact ggctcctttc cgtcgaggac aacggtgtgg
     1441 ggatcccgga ggagatgctg cccaagatat tcgacccctt cttcacgacg aaaggcgcgg
     1501 ggagagggac ggggctggga ttggccgttg tctacaacat catacgccag cattcgggct
     1561 ttatcgatgt cgtgtcgtct cccggtgtgg gcacgatctt caattgtttt ctcccctgtc
     1621 ttcaagacgc cgctcgcgag aaaggagcag gcaaggaaca gggcccgccc cgcggggagg
     1681 gggttgtgct catcgtcgac gacgaagcgg gcatgcgtga ggtcgccgga accatccttc
     1741 aggagctggg ttacagtgtt ctcctcgcgg aggacggcga cgaggcgctg ggggtcttcc
     1801 gcgagcgaaa agacgaaata gacatggtgc tcctcgatgt catcatgccc aggagatcgg
     1861 gccgacaggt ctacagcgag atgaagaaga tcgatcccgg cgtgaaagtg ctgttcgcgt
     1921 caggctactg gcaggacggc ggggacgagg acctctcctc cctcgacgac gcccatttca
     1981 tacagaaacc ctacaccctc caggccatcg ccgaagcggt aaggaaacgg atgacataag
     2041 gactggcttg agccgcttga atcgcttaat cgcttgacag gctgttgata aacccatttt
     2101 ctacgtcatt ccggcgaagg cc
//
LOCUS       DAOQTR010000414         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_65879_length_2122_cov_3.892598, whole genome shotgun sequence.
ACCESSION   DAOQTR010000414 DAOQTR010000000
VERSION     DAOQTR010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65879_length_2122_cov_3.892598"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1447)
                     /locus_tag="PKN85_09285"
     CDS             complement(68..1447)
                     /locus_tag="PKN85_09285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT44644.1"
                     /translation="MDATASLKRNTLLAAVMSSFLGPFTASSVTVALPRIGTHFAMDA
                     ISLGWVATAYLLAAAAFLVPVGRASDIYGRKKIFLWGMVFINLSSLLAGFSSSGTELI
                     LWRIVQGIGSSMIFGTGVTIVSSVYPASERGKALGIVLTAVYVGVSSGPFIGGVLTDL
                     LGWRSLFFTNVAIGMVVIVTILWKIKEEWVEAKGESFDMTGSILYVVAFTAAMYGLTA
                     LPSAKGISFFSGGLVGLFLFGVWELKIEHPVLEMRLFMKSRVFLFSNLAALINYSATF
                     AVTFLISLYLQYIKRLSPQTAGLVLISQPLMQAVLSTFTGRLSDRVEPRVVSSAGMVS
                     LMIALLLFGFLDEGTRTWLVVLNLLFLGFGFALFVSPNTNAIMSSVDKKFYGVASGTM
                     ATMRVIGQTLSMGIVMFVFTLFMGRIQITEQYYGQFLRSTKTTFFIFAFLSFLGIFAS
                     LARGRIHNR"
BASE COUNT          545 a          573 c          612 g          392 t
ORIGIN      
        1 aatgacgtag aaaatgggtt tatcaacagc ctgtcaagcg attcaagcca ttcaaaccct
       61 caagcggtca tctgttatgt atcctccccc ttgccagcga cgcgaagatg ccgaggaagg
      121 acaggaaggc gaagatgaag aaggtcgtct tcgtgcttcg caggaattgc ccgtagtatt
      181 gctccgttat ctggatcctc cccatgaaaa gcgtgaaaac gaacatgacg atgcccatgc
      241 tcagtgtctg ccctatgacg cgcatcgtcg ccatggtgcc cgaggccact ccatagaact
      301 tcttgtccac ggagctcatg atggcgttcg tgttgggaga gacaaaaagg gcgaaaccga
      361 accccaggaa aagaaggttg aggacgacaa gccaggtccg ggtgccttca tcaaggaaac
      421 cgaaaagaag gagggcgatc atgagggaga ccattcccgc ggaggacacg acacggggct
      481 ccacacggtc cgacaatctt cccgtgaagg tcgacaggac ggcctgcatg agcggctgtg
      541 agataaggac aaggcctgcc gtctgggggc tgagcctttt gatgtactgc aggtagaggc
      601 tgatgagaaa ggtgacggca aaggtggcac tgtagttgat cagggcagcg aggttggaaa
      661 agagaaagac cctgcttttc atgaaaaggc gcatttccag aacggggtgc tcgatcttga
      721 gctcccatac gccaaagaga aaaagcccca caagaccacc ggaaaagaag gagatcccct
      781 tcgccgacgg gagggcggtc agaccgtaca tggcggccgt gaaggccacg acgtaaagga
      841 tggaacccgt catgtcgaaa ctttcgccct tcgcctcgac ccactcctct ttgatcttcc
      901 agagtatggt cacgatgacg accatgccta tggcgacgtt cgtgaagaaa aggcttctcc
      961 agccgagaag gtccgtgagg acgcccccga tgaaaggccc cgaagaaaca cctacgtaga
     1021 cagccgtcag aacgatgccg agggccttgc cacgttcgct cgcggggtag acagaggaga
     1081 cgatggtcac gcccgtcccg aatatcatcg aactgccgat gccctggacg atcctccaga
     1141 ggatcaactc ggtccccgaa ctggaaaaac cggcaagaag ggaagagagg tttatgaaga
     1201 ccattcccca gaggaagatc ttcttcctgc cgtatatgtc cgaagccctt cccaccggga
     1261 ccagaaaggc cgcggccgca agcagatatg ccgtcgccac ccagcccagc gagatggcgt
     1321 ccattgcaaa gtgtgtccct atccgtggaa gggcaacggt aacggaggaa gcggtgaaag
     1381 gtccgagaaa ggagctcatt accgcggcga ggagagtgtt tctcttgagg cttgccgtag
     1441 cgtccatgga gtgttgtcga cctgacgata tataccacag aagtccccga cagagagaaa
     1501 cttttcgccg acgggtccgc ccgttcccta ctcaagattg tcgatatcgt caaggatcgg
     1561 gatgcacctg gtgggagatc cgtcgtcaca cacgatgagg gggtcattgc agaccacgga
     1621 aggcctgtgg ggcgcatgat gttcgcatgc ctcagggttt tcgtccctgc attgaccctg
     1681 cagatacttg gcacagatga gccggactgt catgaacgtc attctaccac agacggcaga
     1741 tcatttcctt tgccaagacc tgtcgccttt tcgaaggacg ctgcgggaac atccgaaaaa
     1801 cagttgactt ttccccgatt cttcgctaag tttgcgttcg aaacaaatac ataacctgtt
     1861 gagaaagggg tcagtatcca tgggcaaagg tgacaaacgg accaagagag gaaagatctg
     1921 gcgcggctct tccggcaaga cgcggcctaa gggaaagaag accaataaga cggaaaaggc
     1981 agagaaataa cctcaaccag ggggggcgtc ggcccttcat cgtatcccga ggacgcctcc
     2041 acagtccttg acgatccccc tcgtgagatc ggcaagagcg cggctcatcg catccttcgt
     2101 tcccgcgtcg ccaggctcgt ga
//
LOCUS       DAOQTR010000415         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_66134_length_2117_cov_4.145490, whole genome shotgun sequence.
ACCESSION   DAOQTR010000415 DAOQTR010000000
VERSION     DAOQTR010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66134_length_2117_cov_4.145490"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKN85_09290"
     CDS             complement(<1..634)
                     /locus_tag="PKN85_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNT44645.1"
                     /translation="MRLSAGYMIPFIKNRPDWGFVCGRMSALEGKLLPREFFTLIIEM
                     HHTEDVYQHLQGTLIEDYLDPGIPWDDFSAIADECFYDLATSIRAECPSPVPADLFLI
                     RNDYLNIKSALSGIDSRFLPGMIREQTIGAVLDGDYADLPSPFRDRITGAGIDLGELD
                     ESMSDMFVDGAYLRHLLMLADEIDSPLIRSCVQERVLGHAVSSLWRVLRQG"
     gene            complement(631..1215)
                     /locus_tag="PKN85_09295"
     CDS             complement(631..1215)
                     /locus_tag="PKN85_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HNT44646.1"
                     /translation="MSLDKIKEAVLKASRTEAEHISAAAARQAKEKLEIQKENLRREF
                     EYQYEARSRFIEEEYSRKLAHFQGNAGKEYLEARNAFLRAIFDRARQTVLSWPADEYG
                     RVMKGFLEKITQGRKGRIRIHPDDREVFSKLLAGITAGGGEGDVEIDDGSLPERGGFI
                     FISDEFEVDATIETVLQDIERSLLPEIAEDLARE"
     gene            complement(1240..1755)
                     /locus_tag="PKN85_09300"
     CDS             complement(1240..1755)
                     /locus_tag="PKN85_09300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNT44647.1"
                     /translation="MTPEWIEIGRGLCYAGAGLTFGLAGAGSACGIAIASHAGAGVMR
                     EKPELFGRMLIMIALPGTQGFYGFIAAILIMTQTGLIGGGNLKISLGTGLALFFIGLG
                     NGIAQLVSSIKQGEASAAGIALIARRPESAGRAILFPAFVETYAVVALLATILFIIFL
                     TQPSVLQLLPQ"
     gene            complement(1783..>2117)
                     /locus_tag="PKN85_09305"
     CDS             complement(1783..>2117)
                     /locus_tag="PKN85_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44648.1"
                     /translation="GITAFIGDVLSYCRLLALGLTTSIVGTTFNMLGGLLRDIPYVGL
                     FCFILVVVAGHLFNFVISLLGAFVHSMRLIFVEFFGRFYDAGARPFRPLGFDSPLCEM
                     KKEARKNG"
BASE COUNT          431 a          623 c          631 g          432 t
ORIGIN      
        1 gaccctgtct gagcacccgc cacagggacg aaacggcatg tcccaggacg cgctcctgga
       61 cgcaggagcg aatgagggga ctgtcgatct cgtcggccag catgagcagg tggcgaagat
      121 acgccccgtc gacgaacatg tcggacatgg attcatcgag ctcgccgagg tctattccgg
      181 cgcccgtgat ccggtccctg aagggggagg gcaggtcggc atagtctccg tcgagaacgg
      241 cgcctatcgt ctgctccctg atcattcccg gcagaaagcg gctgtctata ccggaaagag
      301 cgctctttat gttgagatag tcgttccgta tgagaaagag gtcagcggga acgggggaag
      361 ggcactcggc cctgatcgag gtggcgaggt cgtagaagca ttcgtcggct atggcgctga
      421 aatcgtccca ggggataccg ggatcgagat agtcctcgat cagcgtcccc tgaaggtgct
      481 ggtatacgtc ctccgtatga tgcatctcta tgatgagggt gaagaattcc ctcggcagca
      541 gctttccttc aagggcgctc atcctgccgc agacgaaacc ccagtcggga cggttcttga
      601 tgaaaggtat catgtagccg gcagacagtc tcattcgcgt gcaaggtcct cggcgatctc
      661 gggcagaagc gaccgctcta tgtcctgtaa tactgtctct atcgttgcgt ccacctcaaa
      721 ttcatcgctt atgaagatga agcctcccct ttccgggaga gaaccgtcat cgatctccac
      781 gtctccctcg ccgcccccgg cggtgatgcc tgcgaggagt ttcgagaaca cctccctgtc
      841 gtcggggtgg atccgtatcc gtcccttccg gccctgggtg atcttttcca ggaaaccctt
      901 catcacccgc ccatactcgt cggcgggcca ggaaagcacc gtttggcggg ccctgtcgaa
      961 gatggcccga agaaaggcgt tcctcgcctc gaggtactcc ttcccggcat ttccctggaa
     1021 gtgggccagt ttccgggaat actcctcctc gatgaagcgg gaacgggcct catactggta
     1081 ctcgaattcc cgcctcaggt tctccttctg gatctccagc ttttctttcg cctgtctcgc
     1141 agcggcggca ctgatgtgct ccgcctccgt ccttgatgcc tttaacaccg cttctttgat
     1201 cttgtcaaga ctcatgtccc gcccggctcc ttcgtccgtc tactgtggca agagctgcag
     1261 cacagagggt tgcgtgagga agattatgaa gaggatggtc gcaaggaggg ccacgacggc
     1321 gtaggtctcg acaaaggcgg gaaagaggat ggcccgtccc gccgactccg gtcgtctcgc
     1381 gatgagcgct attcccgctg ccgatgcctc tccctgcttt atggaagaga cgagctgggc
     1441 gatcccgttg ccgaggccga tgaagaagag cgcgaggccc gttccaaggg atatcttgag
     1501 atttcccccc ccgatgaggc ccgtctgggt catgatgagg atggcggcga tgaagccgta
     1561 gaatccctgc gtgcccggca aggctatcat gatgagcatg cgcccgaaaa gctccggttt
     1621 ttcacgcatc actcccgctc cggcatggga ggcgatggcg atcccgcagg ccgaaccggc
     1681 gcccgccaga ccaaaggtga ggcccgcccc cgcgtagcaa agaccacgac caatctcgat
     1741 ccattctggg gtcattgtat tcttcctcca aagtttttgt gatcatccat tttttcttgc
     1801 ttcctttttc atctcgcaca ggggcgagtc gaacccgagc ggccggaagg gccgcgcccc
     1861 ggcgtcatag aaccgtccga agaactcgac gaatatgaga cgcatcgagt ggacgaaggc
     1921 gcccaggaga ctgatgacga agttgaaaag atggcccgcg acgacgacaa ggataaagca
     1981 gaagagtccc acgtaaggaa tgtccctcaa aagtccgccg agcatgttga aggtcgttcc
     2041 cacgatggac gtggtgagac cgagggcaag caggcggcaa taggagagga cgtcgccgat
     2101 gaaggcggtg atcccgt
//
LOCUS       DAOQTR010000416         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_66550_length_2109_cov_3.620740, whole genome shotgun sequence.
ACCESSION   DAOQTR010000416 DAOQTR010000000
VERSION     DAOQTR010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66550_length_2109_cov_3.620740"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="PKN85_09310"
     CDS             <1..1216
                     /locus_tag="PKN85_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNT44649.1"
                     /translation="EAAQGSYVVVNVDRILLQDGTAPLAIRKFGDMGFERLFDGSRVT
                     FFIDHASPSPRMELSNDHMMIKDFAARFGANVSDVGEGICHQLMSEGVLVPGEVLVGA
                     DSHTCTGGALASFSTGMGSTDIATAMGLGKTWMRVPESFLFRLSGTFPRGVFPKDLIL
                     KIIGMIGADGATYKAMEFSGEAVASMEIEDRMTVSNMAVEAGAKCGLFPSDEMTRAYL
                     GRNGREGSFVEIAPDGDAVYEKVFDMDLGRLAPIVSLPHTVDNTRAVDDSEVRGMTVH
                     QVFIGSCTNGRIEDLRVAAQVLAGRKKHPGVRLLVGPASRDVYRAALREGILEALLNA
                     GATILPPGCGPCIGLHQGILGKGEICVATSNRNFLGRMGNPESGIVLASPATAAASAL
                     AGKISDPREVLR"
     gene            1213..1710
                     /locus_tag="PKN85_09315"
     CDS             1213..1710
                     /locus_tag="PKN85_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNT44650.1"
                     /translation="MTIKGKAWIFGDDISTDHIIPGRFYHLRSNLEELKKHVFDDIAP
                     GFSERIAKGDVIVGGRNFGLGSSREHAPLIIKMAGIDAIVAPSFARIFYRNAINVGLA
                     AIICPVDGISEGDELEIGLDEGSLTNITKGETKRFAPLPAIMRSILDEGGLDHYIKKH
                     GGFTI"
     gene            1710..>2109
                     /locus_tag="PKN85_09320"
     CDS             1710..>2109
                     /locus_tag="PKN85_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNT44651.1"
                     /translation="MDSIRIPVIAGDGTGEDIWNASREIFEEAVLKTTDGAKRVEWIP
                     LLAGLKALDSQNVLLPQETVDAIMESAIALKGPLTTPVGGGFRSVNVYLRQRFDLYVC
                     IRPIRWFRGLPSPLVNPQAVDMVIFRENTED"
BASE COUNT          428 a          584 c          692 g          405 t
ORIGIN      
        1 cgaagcggcg cagggcagtt acgtcgttgt gaatgtggac aggatacttc tgcaggatgg
       61 aacggcgccg ctcgccatca ggaaattcgg agacatgggc tttgaaagac ttttcgacgg
      121 gtcaagggtc accttcttca tcgatcacgc gtcgccgagc ccccggatgg agctttccaa
      181 cgaccatatg atgataaagg acttcgcggc gcgcttcggc gcgaacgtga gcgatgtggg
      241 agagggcatc tgtcaccagc tcatgtccga gggtgttctc gtgcccggtg aggtactggt
      301 tggggccgac tcgcacacct gcacgggggg tgctctggca tccttttcca cgggaatggg
      361 ctccacggac atcgcgacgg cgatgggcct tggcaagacc tggatgaggg tccccgagag
      421 ctttctcttc cgtctttccg ggacctttcc aaggggcgtc ttcccgaaag acctcatcct
      481 taagatcata ggcatgatag gcgccgacgg tgcgacctac aaggcgatgg agttctcggg
      541 ggaggcggtg gcgtccatgg agatcgagga caggatgact gtctcgaaca tggccgtgga
      601 ggccggagcg aagtgcgggc ttttcccgtc ggatgaaatg acccgggcct acctcggccg
      661 gaacggtcgg gaaggttcct tcgttgagat cgcacccgac ggagacgcgg tatatgagaa
      721 ggtattcgac atggacctcg gtcggctggc acccatcgtg agccttcctc ataccgtcga
      781 caacacgcgc gccgtcgacg acagcgaggt tcgcggcatg accgtgcacc aggtcttcat
      841 cggttcctgc accaatggga ggatagagga cctgagggtc gcggcacagg ttctggcggg
      901 ccggaagaag cacccgggag tgcgcctcct ggtcggtccc gcgtcacggg acgtgtaccg
      961 cgcggccttg agagagggga ttctggaggc tcttctcaac gccggggcga ccatactgcc
     1021 ccccggttgc gggccctgca tcggcctgca ccagggtatc ctcggcaagg gcgagatatg
     1081 cgtcgcaacg tcgaacagga attttctggg caggatgggc aacccggagt cgggcatcgt
     1141 cctcgcgtcc ccggcaacag cggcggcaag cgcgctcgcg gggaagatct ccgatccgag
     1201 ggaggttctg cgatgaccat aaaaggcaag gcatggatct tcggtgacga catttcgacg
     1261 gaccacatca tccccgggcg tttctatcac ctgcggtcaa atctggagga gctgaaaaag
     1321 catgtctttg acgacatcgc acccggtttc tccgagagga tagcgaaggg tgacgtgatc
     1381 gtcgggggaa ggaatttcgg cctcggttca agcagggagc atgccccact tatcataaag
     1441 atggccggca tagacgcgat agtggcccct tcgttcgccc gcatcttcta ccggaatgcg
     1501 atcaacgtgg gtctcgcagc catcatctgt cccgtcgacg ggataagcga gggcgatgag
     1561 ctcgagatcg gcctcgatga gggatcgctc acgaacatca cgaagggtga aacgaagcgg
     1621 ttcgctcccc tgccggcaat catgcggagc atactggatg aaggcggtct tgatcactac
     1681 ataaaaaagc acggggggtt caccatttga tggacagcat caggatacct gtgatcgccg
     1741 gtgatggtac gggcgaggat atatggaacg cctcacggga gatcttcgag gaggcggtcc
     1801 tcaagacgac ggatggcgcg aaacgcgtag aatggatccc tctgctcgcg gggctgaagg
     1861 ccctcgattc ccagaacgtc ctcctgccac aggagacggt cgatgcgatc atggagagcg
     1921 cgatcgccct gaaggggcct ttgaccacgc ctgtcggcgg aggtttccgg agtgtcaacg
     1981 tctacctgcg gcagaggttc gatctctacg tttgtattcg gccgatacgc tggttcagag
     2041 gcctgccgag cccccttgtg aaccctcagg cggtggacat ggtgatcttc agggagaata
     2101 ccgaggacc
//
LOCUS       DAOQTR010000417         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_66855_length_2103_cov_3.478027, whole genome shotgun sequence.
ACCESSION   DAOQTR010000417 DAOQTR010000000
VERSION     DAOQTR010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66855_length_2103_cov_3.478027"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PKN85_09325"
     CDS             <1..1575
                     /locus_tag="PKN85_09325"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HNT44652.1"
                     /translation="LTKGIVVITKKDLVDEDMVELVEEDIRDFLKGRPLEDAPVVAVS
                     ATTGENLDLLKGMLRDVSTRIEEKSRGGIFRLPIDRVFTLRGLGTIVTGTCISGSLSV
                     GEEVELFPFGRKARVRSIQAYHEDVKEASAGQRIALNLQGLDRQDVERGSMVARPDTL
                     LLTSRIDASLRLLKLPIKPIRHDSILRFHIATTQEEARLVLLDSDQIEPGQELFVQFV
                     FKKPVVVLPGDRFILRGSYAIQTVGGGRVLDIMPARHKRKSGDLGGIYRVLSEGTDLE
                     KAGYHVLKGGFQGVRQQSLSVLTGKDMPSIERIGEVLQKEAVATLVGRTYLHKDYFDV
                     YQEKIIAFVGEYHRANPLKIGIPKEELRSRLPATDVQVFQAALDECVTSGLIIVEKDR
                     VRSRDHGQGTSQTDEFEKRVLEALLSAGFTPPGLKDLAGIVGVTEKNTRELLERLAFR
                     GGVVKVGQDMYFHRDMIEEVKEKATNYLRNHQEMGPSDFKSELNLSRKFLIPLLEYLD
                     QIKLTIRKGDKRVLRG"
     gene            complement(1804..>2103)
                     /locus_tag="PKN85_09330"
     CDS             complement(1804..>2103)
                     /locus_tag="PKN85_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNT44653.1"
                     /translation="PEAPPEAAPEKDVIAAFKEEMKKKHETSRNRVAVVKASAANVRS
                     GPGLESPRILTIVQGEILPILDERSDSTGMKWYKVSLYGNRSGWIASQVATATTK"
BASE COUNT          476 a          564 c          627 g          436 t
ORIGIN      
        1 ctcacgaagg gcatcgtcgt cataaccaaa aaagaccttg tcgacgagga catggtcgag
       61 ctggtggaag aggacatcag ggatttcctc aagggcaggc cgctggaaga tgctcccgtg
      121 gtggcggtgt cggcgacgac gggggagaac ctggatctcc taaagggcat gcttcgcgat
      181 gtgtcgaccc ggatcgaaga gaaatcgaga ggcggcattt tccggctgcc catcgacagg
      241 gtattcaccc ttcggggcct ggggaccatc gtcacgggga cctgcatttc agggtcccta
      301 tccgtgggcg aggaagtgga gctgtttcct ttcggcagga aggcgagggt gcgcagcata
      361 caggcttatc acgaggacgt caaagaggcc agcgccggcc agaggatcgc ccttaacctg
      421 cagggccttg acaggcagga cgtcgaaagg ggaagcatgg tggcgcggcc cgacacgctt
      481 ctgctcacgt cgcgcatcga tgcctcgctg cggcttctga agcttcccat aaaaccgata
      541 cgccacgaca gcatattgcg ctttcatatc gcgacgacgc aggaggaggc gcgcctcgtg
      601 ctgcttgaca gcgatcagat agagccgggg caggaactct tcgtccagtt tgttttcaag
      661 aaacccgtcg tggttcttcc cggtgacagg ttcatcctgc gcggttccta tgccatccag
      721 accgtgggcg gcggcagggt ccttgacatc atgcccgcga gacacaagag aaagtcaggt
      781 gacctggggg gtatctaccg cgtcctcagc gaagggacgg atctggagaa ggccgggtat
      841 cacgttttga agggcggttt tcagggtgtc agacaacaga gcctgtccgt gcttaccggc
      901 aaggatatgc cttccattga gaggatcggg gaagtcctcc agaaagaggc cgtggcgacc
      961 ctcgtcggca ggacgtatct gcacaaggat tatttcgacg tatatcagga aaagatcatc
     1021 gccttcgtgg gggaatatca cagggcgaat cccttaaaga tcggcatccc gaaggaggaa
     1081 ctccggtcgc gccttcccgc caccgatgtc caggtgttcc aggcagcgct cgatgaatgc
     1141 gtcacctccg gcctcatcat cgtcgaaaaa gaccgcgtca ggtcgcggga ccacggccag
     1201 ggcaccagtc agaccgatga atttgaaaaa cgcgttctcg aggccctcct gtcggcggga
     1261 ttcactcccc ccgggttgaa ggaccttgcg gggatcgtgg gagtcaccga aaagaacacg
     1321 cgggaacttc tcgagcgcct tgccttcagg ggtggtgtgg tgaaagtggg ccaggatatg
     1381 tatttccacc gcgatatgat cgaagaggta aaggaaaagg ccacgaacta tctccgcaac
     1441 caccaggaaa tgggcccctc ggacttcaaa tccgaactca atctctcaag gaagttcctc
     1501 atacctctcc ttgaatacct tgaccagatc aaactcacga tcaggaaggg agacaagagg
     1561 gtgttaaggg gatagagacg gttttaagtt ttaggtttta agtttcaagt aaaagaaaca
     1621 agaaaagaac gggtccgttt gaaatccgtt ggcatcgagc gtcagcttct caagttttct
     1681 gaaaccatct taagtcccaa atgccaagct ttaagttgca gacgagaaga accaatctgt
     1741 cgggaatggt ccggccagcc gttcttccat cttttcctta aaacctaaaa cctaaaacgg
     1801 tctctatttt gtcgttgccg ttgctacctg ggaggcgatc cagcctgagc ggttgccata
     1861 gaggcttacc ttgtaccact tcattcccgt gctgtcgctg cgttcatcga ggatgggcag
     1921 gatctcgccc tgaacgatgg tgaggatccg cggggactcc agaccgggac cgctgcggac
     1981 gttggccgca gacgccttga cgacggcgac acggttccgg gacgtttcgt gcttcttctt
     2041 catctcttcc ttgaaggcgg ctatcacatc cttttcggga gccgcctcgg ggggagcctc
     2101 agg
//
LOCUS       DAOQTR010000418         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_67001_length_2100_cov_2.948166, whole genome shotgun sequence.
ACCESSION   DAOQTR010000418 DAOQTR010000000
VERSION     DAOQTR010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67001_length_2100_cov_2.948166"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKN85_09335"
     CDS             complement(<1..194)
                     /locus_tag="PKN85_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44654.1"
                     /translation="MRATNAIRVVPSPSRLSIPDMELRYRELLAAKIRRSLFRKDSAR
                     GYRLLDPLGYVTGHKRFKTD"
     gene            388..1950
                     /locus_tag="PKN85_09340"
     CDS             388..1950
                     /locus_tag="PKN85_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT44655.1"
                     /translation="MKNLFPYEKDIPAEMLEGIPVAGMDYLLAGRVEGWKGPFREVSS
                     PVLIQGAGGLSRKCIGSVPSMDEEASLGMLRHAREAYSDGMGVWPRLPAAGRAERMST
                     FIVRMEESRERIVRLLQWEIGKTAGDAAGEFDRSVAYARGCVEVFERCGGKPGGLVVD
                     NGIAGRLERVPRGVVLIMGPFNYPLFETMTAFAPAMLAGNTVVIKPPRLGCLFFQYLL
                     RAARDVFPVGSVNIVYGDGRRIIPPLLSSGMVDLFYFIGTSPVAGYLKGLHPKPHRLK
                     CILGLEAKNPAIVLPDADMDRAASECIRGALTFNGQRCAALKIFFVHRSVAGEFNERL
                     SEAVRGLKHGMPWEDGVFVTPLAEHDRAQYLSGLVEDAMNLGAGIVNEGGGETTGTFF
                     SPAVMYPASTHMRVCREEQFGPVVPVVPYDDIDESLRYITESPYGQQVSLFGKDRETL
                     KKLTEYLVHHVSRVNINCQCQRSPDTVPFTGRKDSAEGSLSISEAVDAFTVPTFIATR
                     NADGNVDIIEEL"
BASE COUNT          416 a          589 c          665 g          430 t
ORIGIN      
        1 atatccgttt tgaaccgctt atggccagtg acataaccga gcggatcaag gaggcgatag
       61 ccgcgagcac tgtccttccg aaatagcgat cttcttattt tcgcggcgag gagttcccgg
      121 taacgcagtt ccatgtccgg gatactcagg cgggacggtg atggtaccac acggatcgca
      181 tttgtcgccc tcatcctgcc ttcaggctcc atgaccggca gttgccgggt agaagagggt
      241 ttgcaactcc agccgggacg tgcttcgccg acttcatgga ccggggagtt ttttctttga
      301 tgttttcccg gttttctacc tataatagac aacccctgca ccgaagaaac tggagttgtg
      361 acggatagct aaagggatga cagcggcatg aagaaccttt ttccatatga gaaggacatt
      421 ccggcggaaa tgctcgaggg catacccgtc gcggggatgg actatcttct cgcgggcagg
      481 gtggagggat ggaagggtcc cttcagggaa gtttcctctc ccgtgctcat acagggcgcg
      541 ggaggtttgt cccggaagtg catcgggtcc gtgccttcca tggacgagga ggcgtcactc
      601 ggcatgctca ggcacgcccg cgaggcgtat tccgacggga tgggggtgtg gccgcggctg
      661 ccggcggccg gcagggcgga gaggatgagc accttcattg tccggatgga ggagtcaagg
      721 gagaggatcg ttcgtcttct ccagtgggag atagggaaga cggcggggga cgcggcaggt
      781 gagtttgacc gttccgtggc gtatgcccgg ggatgtgtgg aggtcttcga acggtgtggc
      841 ggtaagccgg gagggcttgt cgtggacaac ggcatcgccg gcaggctcga aagggtgcct
      901 cgaggggtcg ttctcatcat ggggcctttc aactatcccc tctttgagac catgaccgcc
      961 ttcgccccgg cgatgcttgc gggtaacacg gtggtcatca agccaccccg gctcggatgt
     1021 ctcttcttcc agtatcttct gcgggctgcc cgggatgttt tcccggtcgg atcggtcaat
     1081 atcgtctatg gggacggcag gaggatcata ccacccctct tgtcgtcggg gatggttgac
     1141 cttttctatt tcatcgggac gagccccgtt gccggatatc tcaagggact tcacccgaag
     1201 ccgcaccgtc tcaagtgtat cctcggcctc gaggcgaaga accccgccat cgtacttccc
     1261 gacgccgaca tggacagggc ggcctcggaa tgcataagag gggctctgac gttcaatgga
     1321 cagcgctgtg ccgccttgaa gatattcttc gtgcatcgga gcgttgccgg ggagttcaac
     1381 gaacggctca gcgaggcggt gcggggtttg aagcacggga tgccctggga ggatggtgtc
     1441 ttcgtgacgc cccttgccga gcatgatcgc gcgcagtacc tgtcagggct ggtggaggac
     1501 gccatgaacc tcggtgcggg gatcgtcaat gagggcggag gcgaaacaac ggggaccttc
     1561 ttctctccgg cagtcatgta ccccgcttcg acccacatga gggtctgccg cgaggagcag
     1621 ttcggacccg tcgtgccggt ggtgccctat gacgacatcg atgagtccct caggtatatc
     1681 accgagtcgc cgtacggcca gcaggtcagc ctcttcggca aggacaggga aaccttgaag
     1741 aaactgacag agtacctcgt tcaccacgtg tcgcgggtca acatcaattg ccagtgtcag
     1801 cgcagtcccg acacggtccc attcacgggg agaaaggatt ccgcggaagg gagcctctcc
     1861 atctccgaag ccgtcgacgc cttcaccgtc cccaccttca tagccacgag gaacgccgac
     1921 ggaaacgtcg acatcatcga ggaactttag gaaccgtccc acgccgcagg ctccacctcg
     1981 aacgcggaag gacagaacag ccctcggcga taattgacaa cgcgaagcac tgtctccatc
     2041 tttttcctga gacctgagac ctgagacctg agacctgaga cctgagacct gagacctgag
//
LOCUS       DAOQTR010000419         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_67625_length_2088_cov_3.246434, whole genome shotgun sequence.
ACCESSION   DAOQTR010000419 DAOQTR010000000
VERSION     DAOQTR010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67625_length_2088_cov_3.246434"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKN85_09345"
     CDS             <1..208
                     /locus_tag="PKN85_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44656.1"
                     /translation="GWLVNEREEEAVLTVVVTLFDANGQRLADYSDVIAVEAGEKGTC
                     DVKLVDFQETAETYTIAVTDMELA"
     gene            222..902
                     /locus_tag="PKN85_09350"
     CDS             222..902
                     /locus_tag="PKN85_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNT44657.1"
                     /translation="MDDNDSINKPFSELSQFLKEHRITVKKDASPAPASKTAAAPEDD
                     EHLLSEAMEGVRTIRQGEKRIKVKRPAPVAGPPRESEEEKLLQEVLADHKTINVTNLP
                     EYMEGYVEGINPLIMEKLRKGEFSVQQVIDLHGLSIEGARETFEYFLSNAVKKGLRCV
                     KVIHGRGLRSKRDPTIKDYLKTWILRAMHRKWVTAFSNATMSDGGPGATYILLRSKPE
                     KKRIRIVG"
     gene            1202..1429
                     /locus_tag="PKN85_09355"
     CDS             1202..1429
                     /locus_tag="PKN85_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNT44658.1"
                     /translation="MPTKNARVNVVLEKPLYAAVDEIAKRQGLSRSMVVRDLVREAIE
                     LREDLALAAFAERREQSFDTKKAITHEDVWG"
     gene            1497..1715
                     /locus_tag="PKN85_09360"
     CDS             1497..1715
                     /locus_tag="PKN85_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT44659.1"
                     /translation="MATRIREAIETRLLTAPQEYGEPLRRTLKGYWKLRVGDYRVVFR
                     VKDKDILILGIIHRKEVYQVIGKKRIAD"
     gene            complement(1794..>2088)
                     /locus_tag="PKN85_09365"
     CDS             complement(1794..>2088)
                     /locus_tag="PKN85_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44660.1"
                     /translation="LIVVKNRAWRYGFLCVLIAESLLFAAIMLSTRTMSGAVAVGVLS
                     FIPLIPALSFFLQFRRAENVEVYGGEYESKWTVWYLLGIGLLMIALIFYLGSL"
BASE COUNT          570 a          586 c          566 g          366 t
ORIGIN      
        1 gggctggctc gtcaacgaaa gggaggagga ggcggtcctc accgtcgtcg tcacgctctt
       61 cgacgcgaat ggacagcgtc tcgccgatta ctctgacgtg atcgccgtag aggcgggcga
      121 gaaaggcaca tgcgatgtca agctcgtcga tttccaggaa acggctgaga cttacactat
      181 agccgtgacc gatatggaac ttgcctgaag agaaccggtc catggatgac aacgattcca
      241 tcaacaaacc cttttccgag ctttctcaat ttctcaagga acaccggatc acggtgaaga
      301 aggacgcgtc tccggcaccg gccagcaaga ctgccgccgc gcccgaggac gacgagcacc
      361 tcctcagcga ggcgatggag ggcgtgcgaa cgatccggca gggagaaaag aggataaagg
      421 taaaaagacc ggcacccgtc gcgggtcccc ccagggaaag cgaggaagag aagctcctgc
      481 aggaagtgct ggcggaccac aaaaccatca acgtcacgaa cctccccgaa tacatggaag
      541 gctacgtcga aggcatcaac cccctcatca tggaaaaact ccgcaaaggc gaattctccg
      601 tccagcaggt catagacctc cacggcctct ccatagaagg cgcccgggaa accttcgaat
      661 acttcctcag caacgccgtc aagaaaggcc tcagatgcgt gaaggtgatc cacggcaggg
      721 gcttaaggtc caagcgcgac cccaccatca aggactacct gaaaacatgg atcctccgcg
      781 ccatgcaccg caaatgggta accgccttct ccaacgccac aatgtccgac ggcggccccg
      841 gcgccaccta catcctcctg agaagcaagc cggagaagaa gaggataagg atagtggggt
      901 gaagaaggct tgagccgctt gaaccgcttg aaccgcttga acggggaaag acccgccctg
      961 gaacagtttg agtcggtttc gggcaaaaat ccccttcaac catgcatttt agaagataga
     1021 cttccaatat gcatggttac gttgcgtccc ccctcttccg tcgtggagca tgaaagcgtg
     1081 tggggtccca aagttaccgc tccacagcct ggtccacgag cgcttccctg actcccggtc
     1141 ctgcaggttc ccttgacgtg gtgtatcata agtgttacac tgacagacga aaggagctat
     1201 catgccgaca aagaatgcgc gggtaaacgt ggtattggag aaaccccttt acgcggcggt
     1261 ggatgagata gcgaagagac aggggctgtc cagatccatg gtggtcaggg acctcgtcag
     1321 ggaagcgatc gagcttcgcg aagacctggc ccttgccgca ttcgccgaac gccgggaaca
     1381 gagcttcgat acgaaaaagg ccatcaccca cgaggacgtg tggggctgat gtggagtacc
     1441 taccgaagta ccatccatcc gtcaaaacag aaggcatccc caaacttgac aagcgaatgg
     1501 ccacgaggat aagagaggcc atagaaacgc gactcctaac agcaccccag gaatacggcg
     1561 aacccctcag gagaaccctg aagggatact ggaaactccg tgtcggtgac taccgcgtgg
     1621 tgttccgggt taaggacaaa gatatcctga tcctcgggat cattcacagg aaagaggtct
     1681 atcaggtcat cggaaagaaa aggattgcgg attagacagg accagcggcg tcctcaaata
     1741 tctgatcccc gaaaaacaac ccttccttct tcctgagtgt cctccaagat tcgtcagaga
     1801 ctccctaaat agaagatcaa agctatcatc agtaggccaa tgccgagtag ataccaaaca
     1861 gtccacttgc tttcatactc gcccccatag acttcgacat tctcggctcg tcggaattgc
     1921 agaaagaagg acaatgcggg gatcagtgga atgaatgaga gaacaccaac ggctacggct
     1981 cccgacatag ttctcgtact gagcatgata gcggcgaaaa gcagcgactc agcgatcaag
     2041 acgcagagaa atccatacct ccaggccctg ttcttgacta ctatgagg
//
LOCUS       DAOQTR010000420         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_67973_length_2081_cov_3.450642, whole genome shotgun sequence.
ACCESSION   DAOQTR010000420 DAOQTR010000000
VERSION     DAOQTR010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67973_length_2081_cov_3.450642"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..509
                     /locus_tag="PKN85_09370"
     CDS             375..509
                     /locus_tag="PKN85_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44661.1"
                     /translation="MKAERLREIVRALMSSRFYFDLDIRERHGLVVHVLRMMEARRAS
                     "
     gene            564..1076
                     /locus_tag="PKN85_09375"
     CDS             564..1076
                     /locus_tag="PKN85_09375"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNT44662.1"
                     /translation="MDRTLLLNTTFEPISVLSWKKAITLVYLGKVEVLKEYDRKIRGV
                     SIAIRQPAVIRLLTLVRSNHAKVKFSRRNVFLRDDHTCQYCGKKLDAKNLTCDHLVPR
                     SRGGVTEWTNIVTSCISCNLKKGDKLPEEVKMHPRKRPSRPNGFYMFMLNLGVKVPPE
                     YWRDYMFIRN"
     gene            1078..1569
                     /locus_tag="PKN85_09380"
     CDS             1078..1569
                     /locus_tag="PKN85_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNT44663.1"
                     /translation="MDRVWAPWRMEYIAGTKEPGQKRCFLCIDEGKDEESLVIGRLGT
                     AFVIMNRFPYTNGHVMVVPVRHTGLAEDLTDEESLDMMRLVRKMVRVYKEEFSVEGIN
                     IGINVGRAAGAGLEEHIHVHMVPRWFGDTNFMAVTGETRVISEHLMTSYERLKRRFLE
                     TVL"
     gene            1553..>2081
                     /locus_tag="PKN85_09385"
     CDS             1553..>2081
                     /locus_tag="PKN85_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44664.1"
                     /translation="MRQFFDPSRVVLDPRAIAGALTRKEPHELALPERAVITFNEADL
                     RRLTEGKGASIMDAWAPFRMIHLLSGTSTVVTRSPFGGPNIAALMEELSAFGVREFVL
                     WGYCGGMGPDRSVGNVCLAGSALREEGISWHYLEDDSDMVSSLWTDDWTHAAAEAGFP
                     VIDIWTTDALYRETRN"
BASE COUNT          464 a          552 c          623 g          442 t
ORIGIN      
        1 gccctcttct acgacgccgc cgccatgggc gccggggacg tcgtcgtcta ccgctatccg
       61 gaccagaatg ggaaacggtt tcttcgcggg gggaggcaat ccttcctcgg gatcactcat
      121 tttggggaat tcttcttcgg tgtttatctg atttccattc tcatcgaaca cgggcatttt
      181 cgctggctcc ttttgcttta cgtattttgt ccatacttac cacagaacat agcgaaagac
      241 cagagaaaaa ggcaaaggcg gttgcgcgtt gcgcgttgcg cgttgcgcgt taaagattgt
      301 ctctctggca acccgcaacc cgcaacttta tagtttttgc cctttcgtcc gataatcctt
      361 ttcaggagga tgtggtgaag gccgagagac tgagagagat cgtaagggcg ctcatgagca
      421 gccggttcta tttcgacctc gatatccggg agaggcacgg cctggtggtg catgtcctga
      481 ggatgatgga agccagacgg gcttcgtagg catctcaaat cttgacatga gaggttgaaa
      541 cgggttattt ttcaaatgat taaatggacc ggaccctcct gcttaacacg acctttgaac
      601 cgatcagcgt tctgtcatgg aagaaggcca tcacccttgt ataccttggc aaggtcgagg
      661 tcctcaagga gtacgaccgg aagatccggg gcgtctcgat agccattcgc cagcctgccg
      721 tcatacgttt gctcaccctt gtacggagca accacgcgaa agtcaagttc tcccgaagga
      781 acgttttcct ccgggacgac catacctgcc agtattgcgg aaagaaactc gatgccaaga
      841 atcttacctg cgaccacctc gtccccaggt cccggggagg cgtaacggaa tggaccaata
      901 tcgtcacatc ctgcatcagc tgcaacttga agaaggggga caaactgcct gaagaagtga
      961 agatgcatcc aaggaagaga ccttcacggc ccaacgggtt ctacatgttc atgctgaacc
     1021 tcggcgtcaa ggtgccgccg gagtactggc gagactacat gttcataagg aattgatatg
     1081 gatagagttt gggccccctg gagaatggaa tatatcgcgg gcacgaagga accgggtcag
     1141 aagaggtgct ttctctgcat cgacgagggg aaggacgaag agtccctcgt gatcggcagg
     1201 ctcggtacgg cctttgtcat catgaaccgc tttccctaca cgaacggcca cgtcatggtc
     1261 gtgccggtcc gccatacggg cctggctgaa gaccttaccg acgaggaatc cctcgacatg
     1321 atgcgccttg tcaggaagat ggtcagagtc tacaaggagg agttcagcgt cgaggggatc
     1381 aacatcggca tcaacgtggg ccgcgccgcc ggggcggggc tggaagagca catccacgtg
     1441 cacatggtgc cgcgttggtt cggggacacg aacttcatgg ccgtcacggg ggagacgagg
     1501 gtcatctccg agcacctcat gacctcctat gagaggctga agagaagatt ccttgagaca
     1561 gttctttgat ccctcccgcg tggtcctcga cccccgggcg atagccgggg cactcacaag
     1621 aaaagaaccg catgagctcg cactgcccga aagggccgtc ataaccttca acgaagcgga
     1681 cctgaggcga ttgacggaag ggaagggggc atccatcatg gacgcgtggg ctcctttcag
     1741 aatgatccat cttctgtccg ggacgtcgac ggtagtgaca aggtctccct ttggtggccc
     1801 gaatattgcc gcattgatgg aggaactgtc cgcgttcggc gtcagggagt ttgttctctg
     1861 gggctattgc ggaggcatgg ggccggacag gtcggtgggg aatgtctgtc tggcgggaag
     1921 cgcgctccgc gaggagggga tatcctggca ttacctcgaa gacgacagcg acatggtatc
     1981 gtcgctctgg acggatgact ggacccatgc ggcggcagag gcgggatttc ccgtcatcga
     2041 tatctggacg acggacgccc tctaccggga gacccggaac a
//
LOCUS       DAOQTR010000421         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_67985_length_2081_cov_2.939783, whole genome shotgun sequence.
ACCESSION   DAOQTR010000421 DAOQTR010000000
VERSION     DAOQTR010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67985_length_2081_cov_2.939783"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..>2081
                     /locus_tag="PKN85_09390"
     CDS             170..>2081
                     /locus_tag="PKN85_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HNT44665.1"
                     /translation="MKGVPVIVVIFAVICSILFLPGLSHTQKPEDGDKTLSPYFFVKS
                     DDPKLDQLPLKATSAVANISGVIADVVVTQVYKNEGKKALEAIYVFPASTRAAVYGMK
                     MVIGKRVIEAKIKKRDEARKDYEDAKKQGKSASLLEQQRPNVFQMNVANIMPGDEIRV
                     ELKYTEVLVPTDRIYEFVYPTVVGPRYSNKPADGALPSDKWVQNPYLREGEKPTSTFD
                     MTVTVAGGMPIKELGSTSHKVNTAFNGPANAKVTLDRFEKSGGNKDYILRWRLAGDRI
                     QSGLLLYEGEKENFFLCMLQPPKKVKSAQIPGREYIFVVDVSGSMHGFPLDISKRVLK
                     SLIGNLRSTDTFNVLLFSGASRLMSEKSLPATKENVDNAINVIDRQRGGGGTELLPAL
                     DRVLKLPKFDNYSRSIIIVTDGYVMVEEEVFDLIRTHLNDANVFAFGIGSSVNRHIIE
                     GMARVGMGEPFVITKPADAPAQADRFRAMIESPVLTKVKVSFKGFSTYDVEPVSIPDV
                     LADRPVMVFGKYRGEAKGTIMVTGVSGNGRFTESINVAAAKPLKENVALRYLWARHRI
                     TLLSDYNKLHSSDKRVKEVIGLGLAYNLLTAYTSFVAVDNQVRNTGDKPQTVNQPLPL
                     PEGVSDYAVGGSG"
BASE COUNT          461 a          645 c          591 g          384 t
ORIGIN      
        1 taccccgtcc gcccgtttgc ccgaagggca gacccgtcca ctcgttaggc gcctcaggcg
       61 ccgacccgtt ggcacgaagt gccgacgcgc aagcgccatt tggaactttt tcccgccctc
      121 ccgtgtctca tcaaaaagaa tgaaaacagc tatctcaagg aggttgtcca tgaaaggcgt
      181 tccggtcatt gttgttatct ttgccgtcat ctgctcgatc ctgtttctgc cggggctttc
      241 gcacacgcag aaaccggagg acggcgacaa gacgctgtcc ccctatttct tcgtgaaaag
      301 cgacgatccc aagctcgacc agctgccgct caaggccaca tccgcggtag cgaacatctc
      361 cggtgtcatc gcggacgttg tggtgaccca ggtgtataag aatgagggca agaaggccct
      421 cgaggcgatc tatgttttcc ccgcctccac ccgggccgcc gtgtacggca tgaagatggt
      481 catcggcaaa cgtgtcatcg aggcgaagat caagaagcgc gacgaggccc gcaaggacta
      541 cgaggacgcg aaaaagcagg gcaagagcgc gtcccttctc gagcagcaaa ggccgaacgt
      601 cttccagatg aacgtcgcca atatcatgcc cggtgacgag atacgggtgg agctgaagta
      661 cacggaggtc ctcgtcccga cggacaggat atacgagttc gtctatccca cggtggtcgg
      721 tccccgttat tcgaacaaac ccgccgacgg cgcgctgcct tccgacaaat gggtacagaa
      781 cccctatctg cgcgaaggag agaaaccgac gagcaccttc gacatgacgg tcaccgtcgc
      841 gggcggtatg cccatcaagg aacttggttc cacctcccac aaggtgaata ccgctttcaa
      901 cggtcccgcg aacgcgaaag tgaccctcga cagattcgag aagtcaggcg gcaacaagga
      961 ctacatactc cgctggcgcc tcgccgggga caggatacag tccgggcttc ttctgtatga
     1021 gggcgaaaag gagaatttct tcctctgcat gctgcagccc ccgaagaagg tgaagagcgc
     1081 gcagataccc ggcagggagt acatcttcgt cgtcgacgtc tccggatcca tgcacggttt
     1141 tcctctcgac atatcgaaga gggttctcaa gagccttatc ggcaacctcc gttcgacgga
     1201 taccttcaac gtcctgctct tctcgggcgc atcgaggctc atgtccgaaa agtccctccc
     1261 ggcaacaaaa gaaaacgtgg acaacgccat caacgtcatc gaccgccagc gcggcggtgg
     1321 cgggacggag cttctcccgg cgctggaccg cgtcctcaag ctcccgaaat ttgacaacta
     1381 ctcccggagc atcatcatcg tgaccgacgg ttacgtcatg gtcgaagagg aggtgttcga
     1441 cctcatccgc acccacctca acgacgccaa cgtgttcgcc ttcgggatag gcagctccgt
     1501 gaaccgccac atcatcgagg gcatggcccg tgtgggcatg ggcgagccct tcgtcatcac
     1561 gaagcctgct gacgcaccgg cccaggcgga ccgcttccgg gcgatgatag agtcgcctgt
     1621 cctgacaaag gtcaaggtga gcttcaaggg gttctccacc tatgacgtgg agcctgtttc
     1681 cattcccgat gttctcgcag accgccccgt gatggtcttc ggcaagtaca ggggagaagc
     1741 taaaggcacc ataatggtga cgggtgtttc ggggaacggg agattcacgg agtccatcaa
     1801 cgtggcggcc gcgaaaccgc tgaaggagaa cgtcgcgctg cggtatctct gggcgcgcca
     1861 ccggatcacg ctcctctcgg actacaacaa gcttcattcc agtgacaagc gtgtcaagga
     1921 agtgatcggg ctcggcctcg catataacct cctcacggcc tacacatcct tcgtggccgt
     1981 cgacaaccag gtgaggaaca ccggggacaa accccagacg gtgaaccagc cgctgcccct
     2041 gcccgaaggc gtttccgact atgcggtcgg cggctccggc a
//
LOCUS       DAOQTR010000422         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_68091_length_2079_cov_3.307312, whole genome shotgun sequence.
ACCESSION   DAOQTR010000422 DAOQTR010000000
VERSION     DAOQTR010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68091_length_2079_cov_3.307312"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKN85_09395"
     CDS             <1..1093
                     /locus_tag="PKN85_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44666.1"
                     /translation="ATGLGYQVMRALGSRGVRSVVIYDQESEEIGRYSRYVADRIKVP
                     RFIEQPERLLVYLLENGRKWSGTLIMPTKDYGVEFLARYRDMLSRYYIVPGPGLDIIE
                     GILDKNVLYENARRLGIAVPEAFRARSLEELYGFRNRIKFPCLLKPGRAHVFIRRFGF
                     KMFEIESFEQLAGVYRDLTGNVAGDPFGMMICEIIPGPDSERMVQFASYIDRSGEVLA
                     SMTTRKVRQDPPRYGQGRVTKSEKVTDVDELSGRLLKGLGYHGFSEIEWKYDPRDGQY
                     KLIEINPRFIFYTALCTSCGINFPYIEYADLVRHEKIRVHSFRENVYWVHLYKDLLHT
                     VLNHSMEGFSIREYLRPYLGRTSFAIFDP"
     gene            1179..>2079
                     /locus_tag="PKN85_09400"
     CDS             1179..>2079
                     /locus_tag="PKN85_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT44667.1"
                     /translation="MSVIVTNAKNRIAYTVVRSLGGKGIPVYTADFVPLSMSFASRFS
                     KGHFIYPSPFRDQAGFIASIVKNARRLKAEVLIPVFEETFLLSKYKDEISRHVRMAVP
                     DYDQVLTAHNKDRWMPLARSLGVPVPRTFAAAELRKAGPEVAALTYPVLVKPNQGGGA
                     WGIREAATERELEAILSGDTWFGRPWERFSVQEKVAGTVHCVAMLFGRGELKAHVVYR
                     QLRDLPFTGGQATLRVSVDNPAAVGHLRRLLERLAWHGICQADFIVDEATGIPYLIDI
                     NPRLWGSLVQGIASGVDFPYLLYT"
BASE COUNT          428 a          607 c          632 g          412 t
ORIGIN      
        1 cgccactggc ctgggatacc aggtgatgag ggctctcggc agtcggggcg ttcgatccgt
       61 cgtcatctat gaccaggaaa gcgaggagat cggaaggtat tcgagatacg tggccgaccg
      121 catcaaggtc ccgcgcttca tcgagcagcc ggagcgcctc ctcgtctatc ttctggagaa
      181 cgggcggaag tggtccggca cattgatcat gcccacgaag gattacggcg tcgaattcct
      241 ggcgagatac agggacatgc tgtcgcgata ctacatagtc ccggggcccg gcctcgatat
      301 catcgaaggc atcctggaca agaacgtcct gtacgagaat gcccgccggc tggggatcgc
      361 cgttcccgaa gcctttcgcg cgcggtccct tgaagagttg tacgggttca ggaacaggat
      421 aaagttcccc tgtcttctga aaccgggtcg ggcccacgtc ttcataaggc gtttcggctt
      481 caagatgttc gagattgaat cttttgaaca actcgcgggc gtgtacaggg accttaccgg
      541 caacgttgcc ggtgaccctt tcggcatgat gatatgcgag atcatccccg gtccggacag
      601 tgagcggatg gtccagttcg cttcgtacat cgaccgctcc ggggaggtcc tggcctccat
      661 gacgactcgg aaggtcaggc aggacccccc gagatacggt cagggacggg taacgaagag
      721 cgaaaaggtc acggatgtcg acgaactgag cggcaggctc cttaaagggc tcggctacca
      781 cggtttttca gagatcgagt ggaaatacga cccccgcgac ggtcagtaca agctcatcga
      841 gatcaacccc aggttcatat tctataccgc cctgtgcacc tcatgcggca tcaacttccc
      901 ctatatcgag tacgcggacc ttgtccggca tgagaagata cgtgtccact ccttcaggga
      961 gaacgtctac tgggtgcatc tgtataagga cctcctccac acggtgctga accattcaat
     1021 ggaagggttc tcgatccggg aatacctccg cccgtacctg ggcaggacgt ccttcgcgat
     1081 cttcgatccc taggacccca ggcctttctt tgaggaatgg agggagcacg tgggtaacat
     1141 gctcaggaga aggatactta tcttaaggta agcacgccat gagtgtcatc gtcacgaacg
     1201 cgaagaacag gatcgcctac accgtggtca ggagcctcgg cggaaagggg atacccgtct
     1261 acaccgccga tttcgtgccc ctgtccatgt ccttcgcgtc acgtttctcc aaagggcatt
     1321 tcatctaccc gtccccgttc agggaccagg cgggctttat cgcgtccatc gttaagaacg
     1381 cgcggcgcct caaggcggaa gtgctcatac ccgtgttcga ggagacattt ctcctgtcga
     1441 aatacaagga cgagatttcg cggcacgtcc gcatggccgt ccccgattat gaccaggtcc
     1501 tgaccgcgca caacaaggac agatggatgc cgttggcccg gtcgctcggg gtgcccgtgc
     1561 cccggacctt cgcggcggcg gaactgagga aggcggggcc ggaggttgca gcccttacct
     1621 accccgtgct cgtcaagccc aatcagggcg ggggagcctg gggcatcagg gaggcggcga
     1681 cggaaaggga actcgaagcc atcctttccg gggacacctg gttcgggcgg ccctgggaga
     1741 ggttctccgt ccaggaaaag gtcgccggga cggtgcattg cgtggcgatg ctcttcggcc
     1801 gcggagagtt gaaagcccat gtcgtctaca ggcaactgag ggaccttccc ttcacgggcg
     1861 ggcaggcgac gctcagggtc agtgtcgaca accccgcagc tgtgggccat ttgaggagat
     1921 tactggagag gctcgcctgg cacggtatat gccaggcgga tttcatcgtc gatgaggcaa
     1981 cgggcatacc ctacctcatc gacatcaacc cgcgtctctg gggctccctg gttcagggta
     2041 tcgcgagcgg cgtggacttc ccatacctgc tctacaccc
//
LOCUS       DAOQTR010000423         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_68528_length_2071_cov_2.926587, whole genome shotgun sequence.
ACCESSION   DAOQTR010000423 DAOQTR010000000
VERSION     DAOQTR010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68528_length_2071_cov_2.926587"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..1465
                     /locus_tag="PKN85_09405"
     CDS             365..1465
                     /locus_tag="PKN85_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44668.1"
                     /translation="MQIPEARLLEDAFNEFSKASDSIISHYGVLESQIRELKRELEEK
                     NRALERASEYLYTILDSLPVGVVVVDGKSVIFANKNAEDLIPEGIIGNLSGTAERTGE
                     IRCGLGRYRWKTQALANGFEGRHVVAIEDVTEIERMKEHMERDERLMAMGEMAARISH
                     EIKNPLGSMELFLSMLMAGKTRVRDRKYLEYILFGVKTIDRIINNILSYTRPRELSLK
                     EGELRSVVGDTLEFMSVSTSVREIDLEFAPASAGRAEFDPDMLKLVVMNLVSNAMDAV
                     GPGGRIKVDIRENERYGVLVVSDNGPGMTEELRRNIFNPFFTTKDKGVGLGLFIVYNI
                     VKAHGGSVEVESEEGSGSSFLIYIPKERLTVI"
     gene            1462..>2071
                     /locus_tag="PKN85_09410"
     CDS             1462..>2071
                     /locus_tag="PKN85_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44669.1"
                     /translation="MKTNVLVVDDDNHMRIALKESLVRAGYSVALAEDGKKAMVEIDR
                     NIFDLVITDVKMPHLGGIDLLRAIKETSPLMPVILMTGYGTVKDAVQVIKEGAYDYIQ
                     KPFNTDTLYSVVQRALGVNNGKIVFVSRAMKEVLQKAQRVAGSDATVLVLGESGVGKE
                     VVSKFIHENSGRAHMPFVAVNCAALPENLLESELFGYEKGAFT"
BASE COUNT          507 a          556 c          614 g          394 t
ORIGIN      
        1 gtccgaattt gcccatctac aagaaattta cacaattgtg ttgacagtac ctcaacagaa
       61 cgacactcat agaaaaaatg aaaaggctcg gcctggacta gtccggcggg ctgtcaaaaa
      121 aatgacaaac cgaaagacgg cttgaaccgc ttgaaccgct tgaatgggaa aagccgcttg
      181 aatggcttga gtagcttgaa tcgcttgagg gttaaaggac aaagaagaaa gggtttcagt
      241 tgggagatac tatccccggc atgatgccaa aagcgctttg tttttgagcc attcaagcca
      301 ttcaagccat tcaagccatt caagccgtct ttaccttggc acccttcttg caaaatctcc
      361 ccgcatgcag attcccgaag ccagactcct cgaagacgct ttcaacgagt tttccaaggc
      421 ctcagattcg atcataagcc attacggggt gctggaaagc cagatcagag aactcaagag
      481 ggaactcgaa gagaagaaca gggcgctgga gcgggccagc gagtaccttt atacgatact
      541 cgattccctg cccgtcggcg tcgtcgtcgt tgacgggaag tccgtcatct ttgccaacaa
      601 gaacgccgag gacctcatcc ccgaggggat catcgggaac ctgtccggca cggcggagag
      661 gacgggagag ataaggtgcg gtctcggccg gtaccggtgg aagacccagg cgctcgccaa
      721 cggcttcgaa ggcaggcacg ttgttgccat cgaggacgtc accgagatcg aaaggatgaa
      781 ggagcacatg gagcgtgacg agcgcctcat ggccatggga gagatggcgg cccggatatc
      841 ccacgagatc aagaacccgc tgggaagcat ggagctcttc ctttccatgc tcatggccgg
      901 caagacgcgg gtgcgcgacc gcaagtacct cgagtacatc ctcttcggcg tcaagaccat
      961 cgacaggatc atcaataata tactctccta cacccggccg cgggaactgt ccctgaaaga
     1021 gggggaactg aggtccgtcg tcggcgacac gctggagttc atgagcgtgt ccacgtcggt
     1081 gagggagata gaccttgaat tcgcgcctgc ttcggcagga cgggccgagt tcgatcctga
     1141 catgctgaag ctcgtcgtca tgaatctcgt gagcaatgcc atggacgccg tgggtcccgg
     1201 cggcaggatc aaggtggaca tacgcgagaa cgaacggtat ggggtcctcg tcgtcagcga
     1261 caacggaccg ggcatgacgg aggaattgcg aaggaatatc ttcaatccct tcttcacaac
     1321 gaaggataag ggtgtgggcc tgggactctt cattgtctac aacatcgtga aggcccacgg
     1381 cggttcggtc gaggtggagt ccgaagaggg atcgggttca tctttcctca tctatatccc
     1441 caaggagagg ctgacggtca tatgaaaacg aatgtccttg tagtcgatga cgacaaccac
     1501 atgcgcatag cgctgaagga gtctctggtg cgcgcgggat acagcgtcgc cctcgcggag
     1561 gacggcaaga aggccatggt cgagatcgac aggaacatat tcgacctcgt catcaccgac
     1621 gtgaagatgc ctcatctcgg cggcatcgac cttctcaggg ccataaagga gacgtcgccg
     1681 cttatgcccg tcatccttat gacaggatac ggcacggtca aggacgcggt gcaggtcatc
     1741 aaggagggcg cctacgacta catccagaag cccttcaaca cggatacgct ctacagcgtg
     1801 gtgcaacggg ccctcggtgt gaacaacggg aagatcgtct tcgtctcgcg ggcaatgaaa
     1861 gaggtgctcc agaaggcgca gagggtggcc gggtccgacg cgacggttct tgtcctcgga
     1921 gagagcggtg tgggcaaaga ggtggtatcg aaattcatcc acgagaactc cggccgcgcc
     1981 cacatgccct tcgttgccgt caactgcgcc gctctgcccg agaaccttct cgagagcgag
     2041 ctcttcggct acgagaaagg ggccttcacg g
//
LOCUS       DAOQTR010000424         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_68970_length_2063_cov_2.947211, whole genome shotgun sequence.
ACCESSION   DAOQTR010000424 DAOQTR010000000
VERSION     DAOQTR010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68970_length_2063_cov_2.947211"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKN85_09415"
     CDS             complement(<1..355)
                     /locus_tag="PKN85_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HNT44670.1"
                     /translation="MEVKKFVVILLGILVLAAMTSAAFAAAAFHIGICTGTVSQSEDD
                     LRGAEAMIAKYGDVSDGGMIKHLTYPDNFMQEMETTISQIASLADDPLMKVVIVNQAI
                     PGTTEVMRAEFQAKKI"
     gene            720..>2063
                     /locus_tag="PKN85_09420"
     CDS             720..>2063
                     /locus_tag="PKN85_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44671.1"
                     /translation="MIDLTINDAQLERTVGRARERNIVVPTFEQMKDPGKIPMKVREK
                     LKTVGLWDVDPANLFRVTWKNEPVPSGGLFGGVNFLELPGEITGVDARVVALVGKWFP
                     TGSHKVGPTLGCLVPRLVTGQFDPTAQKAVWPSTGNYCRGGAYNSQLLACDSIAILPE
                     GMSRERFEWLKTVAGEVIATPGSESNVKEIFDKTWELRRTRDDVVVFNQFEEFGNYLW
                     HHEVTGSALEEAFSNVAKPGDRFFGVTFTSGSAGTLAAGDHMKKIFPASKIAVGEALQ
                     CPTLLLNGFGAHRIEGIGDKHVPWIHNVRNTDMVIDVDDADCMALIRLFNEPAGRGYL
                     ESRKLPRELIDQLPLMGISSAANVIMAIKMAKYYELTAKDIIFTVFTDSMELYGSRLR
                     EMEESFGVYGPVDAAVDHHRSILGLGTDWMKELSYYDRRGIHNLKYYTWIEQQGRD"
BASE COUNT          469 a          566 c          620 g          408 t
ORIGIN      
        1 atattttttt ggcctgaaat tccgcgcgca tgacctcggt cgtgccggga atggcctggt
       61 tcactatgac gaccttcatg agggggtcat cagcgagaga cgcgatctgg gaaatggtag
      121 tctccatctc ctgcatgaag ttgtcggggt aagtgagatg cttgatcatt ccgccgtccg
      181 agacgtcgcc gtacttggct atcatggctt cagcgcccct gaggtcgtcc tccgattgcg
      241 aaacggtacc cgtgcagatg ccgatgtgaa atgcggccgc cgcgaaagcg gccgaggtca
      301 ttgccgctaa cacgaggata ccgagcagaa tgacaacgaa cttcttcact tccattcccc
      361 cttattgaga ttagttaaac ccacttaaat atcactccaa tgagggtttt tgtcaacttc
      421 gggattgggt ttcgagggtg ttcacaaccg aaaccgcgaa gggaattgtc gtaaaaccaa
      481 cggtgtgtcg gatcatacat cggcctaacg agaacccgtc tgttcaagtc ctcttccatg
      541 accgaccctc acccgtcccc gaatccgagg tcgaccgtag ccggggcgat caaagatccc
      601 ttgtcacgct ccgcacggcc ttgacccgcg gagatattgg ggaaatgata gaaaagaaag
      661 cccaaagcac cggtgaacga gatggacgat gatgaaacct atgggagggg gatggagtaa
      721 tgatcgatct caccattaac gacgcccagc ttgaaaggac cgtgggaagg gccagggaga
      781 ggaatattgt ggtcccgacc tttgaacaga tgaaggaccc cggaaagatc cccatgaagg
      841 tcagggagaa gttaaaaacg gtcggcctct gggatgtcga ccccgcgaac cttttccgcg
      901 ttacctggaa gaacgagccc gtccccagcg gcggcctctt cggcggggtt aacttcctgg
      961 aattgccagg ggagataacc ggggttgacg cccgggttgt ggccctcgtg gggaagtggt
     1021 tccccacggg gtcccacaag gtcggcccca ctcttggctg tcttgtgcca aggctcgtga
     1081 cgggccagtt cgacccgacg gcccagaagg cggtatggcc ctccacgggg aattattgcc
     1141 gcggcggcgc ctacaattct caactcctcg cctgtgactc gatagcgatc ctccctgagg
     1201 ggatgagtcg cgagaggttc gaatggctca agacggtggc gggagaagtg atcgcgaccc
     1261 ccggtagtga gagcaacgtc aaggagatct ttgacaagac ctgggagctc aggaggacca
     1321 gggacgacgt ggtggtcttc aaccagttcg aggagttcgg caattatcta tggcaccatg
     1381 aagtgaccgg ttccgccctg gaggaggcct tctccaacgt cgcgaaaccg ggggaccgct
     1441 ttttcggagt gaccttcaca tcgggttccg cgggcaccct ggcggcgggg gatcacatga
     1501 aaaagatctt ccccgcatcg aagatcgccg tcggtgaggc cctacagtgt cccaccctgc
     1561 tgctgaacgg tttcggagcc caccggatcg aagggatcgg cgacaagcac gttccctgga
     1621 tacacaatgt aaggaacacg gacatggtga tagatgtcga cgacgccgac tgcatggccc
     1681 tgatccgcct tttcaacgag ccggcggggc gggggtatct ggagtcgcgg aagctgcccc
     1741 gggaattgat cgaccaactg ccgctcatgg gcatctccag cgcggccaac gtgataatgg
     1801 ccatcaagat ggcgaaatac tacgaactga ccgcgaagga catcatcttc accgttttca
     1861 ccgactcaat ggagctctac gggtcaaggt tacgggagat ggaagagtcc ttcggggttt
     1921 acggccccgt cgatgccgcc gtcgatcacc acaggagcat cctcggcctg ggaaccgact
     1981 ggatgaaaga gttgtcctat tacgacagga ggggtatcca caaccttaag tactacacct
     2041 ggatcgagca gcagggcagg gac
//
LOCUS       DAOQTR010000425         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_69014_length_2062_cov_3.360239, whole genome shotgun sequence.
ACCESSION   DAOQTR010000425 DAOQTR010000000
VERSION     DAOQTR010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69014_length_2062_cov_3.360239"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..944)
                     /locus_tag="PKN85_09425"
     CDS             complement(9..944)
                     /locus_tag="PKN85_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT44672.1"
                     /translation="MTRSARYAPLLIVISVAFSSFLVRLNNYMVNISLPTITRSFGVT
                     SSDASRIISSYLLIITTTLLLFGKLGDRIGLKRVFISGYVIFTVGSLMCGLSHDIDML
                     IGSRFIQGIGSAMLLAVSFAIISHFLPEGNTGWAFGITSTASALGVATGAPVGGIITG
                     YLSWNWAFFINVPVGIVAVIVAMKCIPGRKPGGSPAPAREEGRSGFDFLGTILSFAGL
                     GLLIYGLNNGNKQGWLSPFVVGCLVSSLILIGVFVAWERRHKDPLLHLDLFLDSRYAF
                     ALIATFLVYMLISGTPFSCLSTWRWSRGSTPRPPA"
     gene            1117..>2062
                     /locus_tag="PKN85_09430"
     CDS             1117..>2062
                     /locus_tag="PKN85_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNT44673.1"
                     /translation="MGAKRMLYLDNLRLSFTFLVILHHVCLTYAANSGWYFYQHLDDP
                     FTNIILNILMGVGRTWVLASFFLISGYFTPGSLDRKGTWWFIKDRFIRIGIPLGIFAL
                     IIRPTMVYLLKRDTLSLQYSYLENIFLLKNAAPGPAWFLEVLLVFSLAYAAWRVLTRS
                     NRQRDREARPFPGSGAIILFILVLAAFTFVMRIYLPSEKQIFHLRLGNYAEYVAFFFG
                     GIVAYRNRWLEKLTDTVGKQWTVITAVAVCVYILFIVQAWGTGENLSFLRGGFSLKTL
                     IATYVGTFIAVGSSICLVYLFRTYLNGQPGVIGPMTQDA"
BASE COUNT          453 a          621 c          572 g          416 t
ORIGIN      
        1 gaggagcatc atgccggcgg cctgggcgtt gagccccttg accacctcca ggtagaaagg
       61 catgagaaag gcgttcccga gatgagcatg tacacgagga aggtggcgat aagggcgaag
      121 gcgtacctcg agtccaggaa aaggtccagg tgaaggagcg ggtccttgtg gcgcctctcc
      181 caggcaacga aaaccccgat cagtatcagg gaggatacaa ggcagccgac gacgaacggg
      241 gagagccatc cctgtttgtt gccgttgttc aaaccataga tgagaagacc cagccccgca
      301 aagctgagga tcgtccccag gaagtcgaac ccgctgcgtc cttcctcccg cgccggcgcc
      361 ggtgaaccgc cgggcttcct gccgggaatg catttcattg ccacgatcac cgcgacgatg
      421 ccgacaggca cgttgatgaa gaaggcccag ttccatgaga gatagccggt gattatcccg
      481 cccaccgggg cgcctgtcgc cacgccaagc gccgaggcgg tggaggtgat gccgaaggcc
      541 cagcccgtgt tcccctccgg gagaaaatgg gagatgatgg caaaactcac ggcgagaagc
      601 atggcgctgc ctatgccctg gatgaaccgc gagccgatga gcatgtcgat atcgtgggac
      661 agtccgcaca tgagcgaccc cacggtgaag ataacatacc ccgagatgaa gacccgtttg
      721 agccctatcc tgtcgccgag tttcccgaaa agcaggagcg tcgtcgtgat gatgagaaga
      781 taggaggata ttatccggga cgcgtcgctg gaggtcacac cgaaggagcg ggtgatcgtc
      841 ggcagggaga tattgaccat gtaattgttg aggcgcacca gaaaggagct gaaggcgaca
      901 ctgatgacga tcagaagggg agcgtatctg gcagaccgcg tcatgaatgc aattacttac
      961 actctttcac ggacttccgc aacgtttttc ttggaaacac aggcccgacc ctctccggat
     1021 ataatcgttg cagtcgaaaa cacgatgtgg cacaatgaat aactctgtat gcaagtcaac
     1081 gcggaaaagg tacgggttcc agagtcgcag cctctgatgg gcgcgaaacg gatgctgtac
     1141 ctcgataacc tcagactctc cttcaccttc ctcgtgatcc ttcaccacgt atgcctgacc
     1201 tacgccgcca acagcggttg gtacttctat cagcatctcg atgacccttt taccaacatc
     1261 attctcaaca tcctcatggg tgtcggcagg acctgggtcc tggcatcctt cttcctcatc
     1321 tcgggatact tcacgcccgg ctcgctggac aggaagggta cctggtggtt cattaaggac
     1381 cgtttcatca ggatcggcat acctctcggc atattcgcgc tcatcatccg ccccaccatg
     1441 gtctacctgt tgaaacggga caccctctct ctgcagtatt cctacctgga gaacatcttc
     1501 cttctcaaga acgccgctcc gggacccgca tggttcctcg aggtgctcct cgtcttctcc
     1561 ctcgcctacg cggcctggag ggtcctaacc cgttcgaacc gacagaggga ccgcgaggcc
     1621 cggcccttcc ccggcagcgg cgctatcatc ctgttcatcc tcgtcctcgc ggccttcaca
     1681 ttcgtaatgc gcatctacct tccctcggaa aagcagatct tccatctccg tctcggaaac
     1741 tacgcggaat atgtggcctt cttcttcggg ggcatcgtgg cataccggaa ccgatggctc
     1801 gagaaactga cggacaccgt cgggaagcaa tggacggtca tcaccgcggt ggccgtgtgt
     1861 gtctatatcc tcttcatcgt tcaggcctgg ggcacgggtg aaaacctctc ctttctgaga
     1921 ggaggctttt ccctcaagac cctcatcgcg acatacgtgg gcaccttcat cgccgtgggc
     1981 agctccatct gcctcgtcta tctcttcagg acatacctca acggccaacc gggcgtcata
     2041 ggaccgatga cgcaggacgc ct
//
LOCUS       DAOQTR010000426         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_69325_length_2056_cov_4.001000, whole genome shotgun sequence.
ACCESSION   DAOQTR010000426 DAOQTR010000000
VERSION     DAOQTR010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69325_length_2056_cov_4.001000"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKN85_09435"
     CDS             <1..368
                     /locus_tag="PKN85_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44674.1"
                     /translation="KDCCFGFNETSLFLRVDLDKRFMDEMDGAVFEVSIIGKDDHKIE
                     YHVRDGSIKAAVPVQAAFDEILELCIPFSDLGLGPKDEAEVWFSLKINEMIVDRIPQR
                     GYLVITVPSETFEAEMWYV"
     gene            complement(411..1703)
                     /locus_tag="PKN85_09440"
     CDS             complement(411..1703)
                     /locus_tag="PKN85_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44675.1"
                     /translation="MSPDPSFYYESAEHRQAIDHLTFFLAQQEGFALIYGDVGSGKTT
                     ISRIFLNSLDHGTYNTALILNPVADEAEFMKEVLGEYSVSDIPETKKELYDRLRGYLL
                     EEFQKGRTNVLIIDEAQLLSYDLLEFIRLLSNIETDKQKILHTVFFAQPEFLDKLKDP
                     AMRHLSQRITVTYRIEPLTYAEAKAYINYRLFKAGAKGPLEFQDRAMKLIHTASRGYP
                     RLINYICDRCLLVLYAKSSFSVDGHVVSKVIIEESIPLSTTKHTDDSKRLALRRPLLI
                     GGIIAVIVIGVAGYYFMITGAFRLFAPTRPQTQQASVARTSPAATAAHTTPPAQAPSA
                     PRPAVAAQSSTPARPAAPAKPAQRDIILKRVYVNTEAANVRLEPDINSARIGVIIKDE
                     LLKVIAEKTGADRMKWYKIKLYEGREGWISETVVTERK"
BASE COUNT          417 a          520 c          633 g          486 t
ORIGIN      
        1 tgaaagactg ctgcttcggg ttcaacgaga ccagcctctt cctgcgtgtc gatctggaca
       61 agaggttcat ggatgaaatg gatggggccg tcttcgaggt gtcgatcatc ggcaaggacg
      121 atcacaagat agagtatcac gtccgtgacg ggtccataaa ggcggctgtt cccgtgcagg
      181 ctgcctttga cgagatactc gaactgtgta tccctttcag cgacctcggc ctcggcccga
      241 aggacgaggc ggaggtctgg ttctccttga agatcaacga aatgatagtc gacaggatac
      301 cccagagagg ctatctcgtg atcacagtcc cctcggaaac gttcgaggcg gagatgtggt
      361 acgtctgaaa gacggcttga atggcttgaa tggcttgaat ggcttgaagg ttatttcctt
      421 tcggtgacta ccgtttctga gatccagcct tcgcggcctt cgtagagttt tatcttgtac
      481 cacttcattc tgtcggcgcc ggtcttctcg gcgatgacct tgaggagttc atcctttatt
      541 atgacgccga tgcgggcgga gttgatgtcc ggttccaatc tgacatttgc ggcttcggtg
      601 ttgacgtaga ccctcttgag gatgatgtct cgttgtgcgg gcttagcggg agcggctggc
      661 cgggcaggtg ttgatgattg tgcggctacg gcgggccggg gcgccgaagg cgcctgtgcg
      721 gggggcgtgg tatgggcggc cgtggctgcc ggtgatgtcc gtgcgacaga tgcctgttgc
      781 gtttgaggtc ttgtcggggc gaaaagcctg aaggccccgg tgatcatgaa gtaatagccg
      841 gccacgccta tgacgatgac ggcgatgatc cctcctataa ggagagggcg cctcaaggca
      901 agcctttttg agtcgtcggt gtgtttcgtc gtggaaaggg ggatgctctc ctcgatgatg
      961 accttggaga ccacgtggcc gtccacggag aagctggact tcgcgtagag gacgaggaga
     1021 cagcggtcgc agatgtagtt gatgagacga ggatagcccc gtgaggcggt gtggatgagc
     1081 ttcatagccc tgtcctggaa ctcgaggggt cccttggccc ctgccttgaa aaggcggtag
     1141 tttatgtagg cctttgcctc ggcgtaggtc aggggttcga tgcgatacgt aacggttatg
     1201 cgctgggaaa gatggcgcat tgcggggtcc ttcagcttgt cgaggaactc cggctgggca
     1261 aagaagacgg tgtgcagtat cttctgtttg tctgtttcta tgttggacag gaggcggatg
     1321 aactcaagga gatcgtagga gaggagctgg gcctcgtcga tgatgagaac attcgtcctg
     1381 cctttctgga attcctcgag gagatagccc ctgagtctgt cgtagagctc tttctttgtc
     1441 tccggtatgt cgctgacgga atactcgccg agcacctcct tcatgaactc cgcctcgtca
     1501 gccacgggat tcaggataag ggccgtgttg taggtgccgt ggtccaggct gttgaggaat
     1561 atccgtgaga tcgtcgtctt tcccgagccc acatcgccat agatcagggc gaagccttcc
     1621 tgctgggcaa ggaagaacgt gagatgatcg atggcctgcc tgtgctccgc ggattcatag
     1681 taaaaggacg gatccgggga catcccgaag ggcttctccg aaaaaccgaa atattcaaga
     1741 tagggtattt ccatctttta gagaatatga cacaaagaag aaaaaaaaga aacaggtaat
     1801 gggtaaggag ccccaccgtc attccgccaa ctcccccacc gtcattcctc caactccccc
     1861 accgtcattc ctccaactcc cccaccgtca ttccggcgaa ggccggaatc caggaagacc
     1921 tctgtcgcgt cggtaagtgg tggtgccaac cgggaaggtc tcaacgtttc ctgcaagatg
     1981 gtctcggatc ccgaccctct cctggatccc ggccttcgag actgtgtcgc aattacctcc
     2041 accccggtct gttagt
//
LOCUS       DAOQTR010000427         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_69586_length_2051_cov_3.981463, whole genome shotgun sequence.
ACCESSION   DAOQTR010000427 DAOQTR010000000
VERSION     DAOQTR010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69586_length_2051_cov_3.981463"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PKN85_09445"
     CDS             <1..1256
                     /locus_tag="PKN85_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT44676.1"
                     /translation="EAPKLNIKRSIELFEEAKTLVPGGVLGARKPGDFIMGEYPIFLE
                     YGKGSRLIDVDGNEFIDFLCGYGPIILGYREDEVDDAVIRQLKDKGFCFSLTQPYQNE
                     LARKLNALIPSAELSIFLKTGSDATTASIRIARAYTGRIKVMRSGYHGWHDWCVEMKG
                     GIPEKFYEDVYEFHYNDLEALEDLMKKHGDQTAAIIMTPFGHPLHQKMQTPKPGFLEG
                     VRKLADTYGAVLVFDEVRTCFRLRMGGAQELYGVKPDLTVLGKGMANGYAISVVCGKT
                     DVMMAAASKLFISSTFFPNSDGYIAALKVIEIMEREKVLDQIWEKGERLLKRIQGVID
                     KYPNTGAELTGVAPMFHVTFTKGDPDTQRDRRTDFYTQMIRKGFFFTPHHHAYLSYRH
                     TEEDLDLTIQAMDESMAYVSEKYKG"
     gene            1268..>2051
                     /locus_tag="PKN85_09450"
     CDS             1268..>2051
                     /locus_tag="PKN85_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT44677.1"
                     /translation="MKDDLNIVGKRVVRSDSLAKVTGEARYTADLTFPGMLVAKVLRS
                     PYPHAKIVGIDVSKALKVPGVKAAISGFDSYGVKWGVFRYTQDHAMLHTDKVRYIGED
                     VAAVAAVDEETAMEALARIKVDYEPLPAVYDPMESMKDGAPQIHDQYKNNINIHIHID
                     VGEVDRVMEKAYLVREDTFKAAGEAYAMMEPYAVVACFKSGYLDLYMPNAGPHVRAKA
                     LSNMLRLPLNRVRIRHINSGGAFGGRSEVAPGDLVASLLTMKS"
BASE COUNT          464 a          597 c          619 g          371 t
ORIGIN      
        1 aagaagcccc gaagttgaac ataaagagaa gtatcgaact cttcgaggag gcaaagaccc
       61 tcgtccccgg cggggtcctg ggagccagga aacccggcga ttttatcatg ggcgagtatc
      121 ccatcttcct tgagtacggg aagggctccc ggctcatcga cgtggacggg aacgagttca
      181 tcgatttcct ctgcggctac ggccccatca tcctcggtta ccgcgaggat gaggtcgacg
      241 acgccgtcat ccggcagctg aaggacaagg ggttctgctt cagccttacc cagccctatc
      301 agaatgagct ggcgaggaag ctcaacgccc tcatcccgtc ggccgagctg agcatcttcc
      361 tcaagaccgg ctccgacgcg acgacggcaa gcatccgtat cgcgagggcc tacaccgggc
      421 gtatcaaggt gatgcgctcc gggtaccatg gctggcatga ctggtgcgtc gagatgaagg
      481 ggggcattcc cgagaagttc tatgaggatg tctacgaatt ccactataat gacctcgaag
      541 cgctcgaaga cctcatgaag aagcacgggg accagacggc ggccatcatc atgacgcctt
      601 tcggccaccc tctccaccag aagatgcaga cgccgaaacc gggcttcctc gagggtgtgc
      661 gcaagctggc ggacacctac ggcgcggtcc ttgtcttcga cgaggtgcgc acctgtttcc
      721 gcctcaggat gggcggggcg caggaacttt acggggtcaa accggacctc accgtgctcg
      781 gcaagggcat ggccaacggg tatgccatca gtgtggtctg cgggaaaacg gacgtgatga
      841 tggccgcggc atcgaagctt ttcatctcct ccacgttctt cccgaacagc gacggctaca
      901 ttgccgcgct caaggtcatc gagatcatgg agcgtgagaa agtgctcgac cagatatggg
      961 aaaagggcga acggctcctc aaaaggatcc agggtgtcat cgacaagtat ccgaacaccg
     1021 gggcggaact cacgggcgtg gcccccatgt tccacgtgac cttcacgaag ggtgaccccg
     1081 acacgcaaag agaccgcagg accgacttct acacccagat gatcaggaaa gggttcttct
     1141 tcacacccca tcaccacgcc tacctcagct acaggcacac cgaagaggac ctcgatctca
     1201 cgatccaggc gatggatgaa tccatggcct atgtgagcga gaagtataaa gggtaggggg
     1261 gacgttaatg aaggacgatc tcaatatcgt aggcaaaagg gttgttcgaa gcgattccct
     1321 ggcgaaggtg acaggcgagg cgcgctatac ggccgacctt acgtttccgg ggatgctcgt
     1381 ggcaaaggtc ttgaggagcc cgtatcccca cgcgaagatc gtgggcatcg atgtttcgaa
     1441 ggccctcaag gtgccaggcg tcaaggccgc catcagcggc ttcgactcct atggtgtaaa
     1501 gtggggtgtt ttcaggtata ctcaggacca tgccatgctc cacaccgaca aggtgcggta
     1561 catcggcgag gacgtcgcgg cggtggctgc cgtcgacgag gagacggcaa tggaggccct
     1621 cgcccgtatc aaggtggact atgagcccct ccctgccgtt tacgacccca tggagtccat
     1681 gaaggacggt gcaccacaga tccatgacca gtacaagaac aacatcaaca tccatattca
     1741 tatcgacgtg ggcgaggtcg acagggtcat ggagaaggcg taccttgtcc gggaggacac
     1801 cttcaaggcg gccggcgagg cctatgccat gatggaacca tacgccgtgg tcgcctgttt
     1861 caagagtggc tatctcgacc tctacatgcc gaacgcgggg ccgcacgtcc gggccaaggc
     1921 cctgtcgaac atgctgaggc tgcccctcaa cagggtgcgc atccgccaca tcaattccgg
     1981 cggggccttt ggaggacgct ccgaggtggc tccaggcgac ctcgtggcat cacttctgac
     2041 aatgaagtcc g
//
LOCUS       DAOQTR010000428         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_70154_length_2041_cov_2.655589, whole genome shotgun sequence.
ACCESSION   DAOQTR010000428 DAOQTR010000000
VERSION     DAOQTR010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70154_length_2041_cov_2.655589"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PKN85_09455"
     CDS             complement(<1..831)
                     /locus_tag="PKN85_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT44678.1"
                     /translation="MLENREGLKRWGMKNVAICLCLALCLFACDKKDDSKVLVTINDD
                     KVTMKEFNKELDRIPMNMKMMVASQSGKKAYLDRLIIKKLLLAEAQKANIEKDKDFEN
                     RLAEIKEQLIIESLLKKKLTAGANITEDDLQKYYEKNKEKFRKDAEINTRHILVKSEE
                     QARQVKEKFQAGEDFTELAKKYSIDPNAKVTGGEVGFHPKGTLLPEYEAAAFKLTKIG
                     QTSGIVKTQFGYHIIRLEGIKPPAYVPFAEVKDFIKQQLMQEKQKEVLDKYIEELKKS
                     A"
     gene            complement(918..>2041)
                     /locus_tag="PKN85_09460"
     CDS             complement(918..>2041)
                     /locus_tag="PKN85_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT44679.1"
                     /translation="GIRDLSVIDTPPLDRHAVRTTVVKFNDAVVARGIRNELERSGQV
                     FFVHNFVHNIGVVHDHLARIIPEARIAVAHGQMSGSRLEKIMVDFIEKRYDILLSTNI
                     IESGLDISNVNTIFINNAHRMGLADLYQLRGRVGRSTRQAFAFLLVPDEEKLTRDARL
                     RLKILEELTDLGSGFQIANYDLEIRGAGNLVGKEQSGNVNLIGFELYCDMLEETMMRL
                     RNKGPIPAEEINVEINIPVNAYIPDTYIQDATQKLLAYKRLSRVRDEEELREMEGELK
                     DRYGALPSPLVNLLEVISLKSLLTRLKVRKAEKSEGQVILHVTDRTPLDMKRLLALAM
                     DDGKGIKLLPDGRIVIRSGLHEGELMNFIRNILMEIAPL"
BASE COUNT          350 a          580 c          560 g          551 t
ORIGIN      
        1 cgcgcttttc ttcagttcct cgatgtattt gtcgaggact tccttttgtt tctcctgcat
       61 gagttgctgc ttgatgaaat cctttacctc ggcgaagggg acgtaggcgg gtggctttat
      121 cccctcgagt cgtattatgt gatacccgaa ctgggtcttg acgatcccgc tcgtctgacc
      181 gatcttcgtc agtttgaatg ccgctgcctc gtattcgggc agaagggtgc ccttgggatg
      241 gaaaccgacc tcgccacccg tcactttggc gttggggtcg atagagtatt tcttggcgag
      301 ttccgtaaaa tcctcaccgg cctggaactt ctctttcacc tgtctggcct gttcctcgga
      361 tttcacgagg atgtgccgcg tgttgatctc cgcgtcctta cggaatttct ccttgttctt
      421 ctcgtagtac ttttggaggt cgtcttcggt gatattggcg cccgccgtta gttttttctt
      481 gaggagcgat tcgatgataa gctgttcctt gatctccgcc agcctgttct cgaaatcctt
      541 atccttttcg atgttggcct tctgggcttc cgcgagaagg agcttcttta tgatcaggcg
      601 gtcgaggtag gccttcttgc cgctctggga ggcgaccatc atcttcatgt tcatcggtat
      661 tctgtcgagt tccttgttga actccttcat cgtgaccttg tcgtcgttta tggtgacgag
      721 caccttgctg tcatccttct tgtcgcacgc aaagagacac agtgccaaac acagacagat
      781 tgccacgttc ttcattcccc acctctttag cccttctcga ttttccagca tatatcgacc
      841 tctatcaccg gctgctcccg accggggcga cccgaaacac acgatggcga tcaattagcc
      901 tgtaacgaaa tcttatatca cagcggtgcg atctccatca atatatttct tatgaaattc
      961 ataagttccc cctcatgaag gccggaacgg atgacgatcc gcccgtcggg caggagcttg
     1021 atgcctttgc cgtcgtccat ggcaagggcg agaagccttt tcatgtcgag gggcgtccgg
     1081 tctgtaacat ggagaatcac ctgtccctcg ctcttctcgg ccttacggac cttcagtcgc
     1141 gtcagcaggg acttcaggga aatgacctcg aggagattga cgagcgggga gggaagggcg
     1201 ccataccggt ctttcagctc gccctccatc tccctgagct cctcttcatc gcgcacacgg
     1261 gagaggcgct tgtatgcgag gagcttctgc gtggcatcct ggatgtacgt gtcgggtatg
     1321 tacgcgttca cggggatgtt tatctcgacg ttgatctctt cagcggggat ggggcccttg
     1381 ttcctgagcc tcatcatggt ctcttcaagc atgtcgcagt aaagctcgaa gccgatgagg
     1441 ttcacgttgc ccgactgctc cttgccgacc aggttccccg cgccgcgtat ctccaggtcg
     1501 tagttggcta tctggaagcc cgaaccgagg tcggtcagct cttcgaggat cttgagcctg
     1561 aggcgggcgt cccttgtcag tttctcctcg tcgggaacaa gcaggaacgc gaaggcctgc
     1621 cgggtgctcc gtccgaccct gcccctgagc tggtagaggt cggcgaggcc catcctgtgg
     1681 gcattgttga tgaagatggt gttgacattg gatatgtcga gccccgattc gattatgttc
     1741 gtcgagagga gtatgtcgta gcgcttctct atgaagtcca ccattatctt ttccagccgg
     1801 ctcccgctca tctgcccgtg ggcgacggcg atgcgggcct ccggtatgat cctcgccagg
     1861 tggtcgtgaa cgacccctat gttgtgaacg aagttgtgca cgaagaagac ctgcccggac
     1921 ctttcgagct cgttgcggat cccgcgggcc accaccgcgt cgttgaactt caccaccgtt
     1981 gtcctcacgg cgtgccggtc gagcggcggc gtgtcgatga cgctgaggtc cctgataccc
     2041 g
//
LOCUS       DAOQTR010000429         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_70398_length_2036_cov_3.621403, whole genome shotgun sequence.
ACCESSION   DAOQTR010000429 DAOQTR010000000
VERSION     DAOQTR010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70398_length_2036_cov_3.621403"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKN85_09465"
     CDS             complement(<1..594)
                     /locus_tag="PKN85_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44680.1"
                     /translation="MKMGMGMEGNRRLFSLVGITALAGILLCGGGCAPTIRTVSEDVI
                     EAEAVRIAREEGREEGRREGSRLCKEELDERLRDFVRRYRDELLYLELTKGGAIVPAQ
                     VRLVYNPAKISRDGSSYSAPTLVWKIVSPPRFVSDESGEGWLGRDRANFCYFLVDSFA
                     TEAEAFQFVGNSPKPDDVFLTTAPHGDAGKWAVIGKTF"
     gene            complement(591..863)
                     /locus_tag="PKN85_09470"
     CDS             complement(591..863)
                     /locus_tag="PKN85_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44681.1"
                     /translation="MARRSETAVSCCATLLVLVWGFISPLVAQAQEGPQGSAPDAGRS
                     SLIRALSRQIAFVRSFSVEYPFQFRQEAGAALTGAPAAGDRKARGE"
     gene            complement(835..1614)
                     /locus_tag="PKN85_09475"
     CDS             complement(835..1614)
                     /locus_tag="PKN85_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44682.1"
                     /translation="MPQETPKQQAEGQIVVPPFDLPEKDIVEMKEKVQRLKPLIEKDH
                     ELARARERDVDEVPGAVPTIHLSYGYGSVINLPYSFSVDDIAIGAREKFNIETRGNSL
                     IIFPVREFKSTNIIVFERTQAGDQLPHHYLLVENGASGEADLTVNVRRSGMGGVSDIT
                     DAMVRVITTQRIPEKGTAEDILLEGRSPSLTNLSDYPFIRMMKLQKPELHVFMIAGKV
                     MPVGDADFALDLGNGTSVVASRSREITVRRVGDGKTFRNSR"
     gene            complement(1628..>2036)
                     /locus_tag="PKN85_09480"
     CDS             complement(1628..>2036)
                     /locus_tag="PKN85_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44683.1"
                     /translation="IFCRDAIRDILGWTYVEVRSQSDLANRISRVSKRFDPASFKAFY
                     EPYRDNYLKSLSDQKLIVTAEWNALKDSKIRGKEAVVLASVRLNAVRVVGEEAASEDL
                     KLKTYEIKLYKGARSVENPFGLYIVRFSEPAGG"
BASE COUNT          399 a          645 c          552 g          440 t
ORIGIN      
        1 gaaggtcttc ccgatcaccg cccattttcc ggcgtcgccg tgaggcgctg tcgtcaggaa
       61 gacgtcgtcg ggcttcgggg agttgccgac gaattgaaaa gcctccgcct ccgtggcgaa
      121 tgaatctacc aggaaatagc agaaattggc ccggtccctg ccgagccaac cctcgcctga
      181 ttcatcggaa acgaaccggg gcggcgacac gatcttccag acgagggtcg gtgccgagta
      241 gctgcttccg tcccggctta tcttggccgg attgtagacc agcctcacct gtgccggtac
      301 aatggcaccg cccttcgtca attcgagata taaaagctca tcgcggtacc tccgaacgaa
      361 gtcccgcaat cgctcatcca gttcttcctt acagaggcgc gacccctccc gtctgccctc
      421 ttctctgcct tcttcccgcg cgatccgcac ggcttccgcc tcgatgacat cctcggatac
      481 tgtcctgatg gtgggagcgc atccgcctcc gcagagaagg atccccgcca gggccgtgat
      541 accgacgagc gaaaaaaggc gcctgtttcc ttccatgccc atacccattt tcattccccg
      601 cgcgccttcc tgtcaccggc agcaggcgca ccggtcaatg ctgccccggc ctcctgccgg
      661 aactggaatg ggtattccac cgagaaggac ctcacgaagg cgatctgccg cgaaagggcg
      721 cgtatgaggg acgagcggcc ggcgtcggga gcagagcctt gcggtccctc ctgcgcctgc
      781 gccacgagcg gcgatatgaa gccccaaacc agcacaagaa gagttgcaca gcagctaacg
      841 gctgtttctg aacgtcttgc catcgcccac cctccttacc gttatttccc ggctccgcga
      901 tgccacaacg cttgtcccgt tgccgaggtc cagggcaaaa tcggcatcgc ccacgggcat
      961 caccttcccg gctatcatga agacatgaag ctccggtttc tggagtttca tcatccgtat
     1021 gaaaggatag tcactgagat tcgtcagtga aggcgaccgg ccctcgagga gaatgtcttc
     1081 tgctgtgccc ttctcgggga tgcgctgtgt cgtgatgacg cgcaccattg cgtccgttat
     1141 gtcggacacg ccacccatgc ctgaccgcct tacgttgacg gtgaggtccg cctctcccga
     1201 tgccccgttc tcgacaagca aataatggtg gggcaactgg tcacccgctt gcgttctttc
     1261 gaaaacaata atgttggtcg acttgaattc tctcactgga aagatgatga gggagttgcc
     1321 tctcgtctcg atgttgaatt tctcccttgc gccgatcgcg atatcgtcga cgctgaaact
     1381 gtaagggaga ttgatgacgc tgccgtaccc gtatgagaga tggatggtgg gcaccgcacc
     1441 cggcacttcg tcgacatcgc gctccctcgc ccgtgccagc tcgtgatcct tttctatgag
     1501 aggcttgagg cgctgcacct tctccttcat ctcgacgatg tccttctcgg gaagatcgaa
     1561 gggagggaca acgatctgcc cctcagcctg ctgcttcggg gtctcctgag gcatgtccgg
     1621 cgcggcctca gccaccggca ggctcagaaa aacggacaat atacagaccg aaagggttct
     1681 ccacactgcg cgctcctttg tacagcttga tctcgtacgt ctttagcttg aggtcttcgc
     1741 tcgccgcttc ctctccaacc acacgaacgg cattgagtct gaccgaggca agaacgaccg
     1801 cttccttgcc gcgtatcttc gagtccttga gagcattcca ctcggcggtg acgatgagct
     1861 tttggtccga cagggacttg agataattgt cccggtacgg ctcgtaaaaa gccttgaagg
     1921 acgcgggatc gaaacgcttc gacacgcgac ttatccggtt ggcaagatcg ctttgggacc
     1981 tcacctccac ataggtccag ccgaggatgt ccctgatcgc gtccctgcag aagatc
//
LOCUS       DAOQTR010000430         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_70831_length_2028_cov_3.347694, whole genome shotgun sequence.
ACCESSION   DAOQTR010000430 DAOQTR010000000
VERSION     DAOQTR010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70831_length_2028_cov_3.347694"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKN85_09485"
     CDS             <1..391
                     /locus_tag="PKN85_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44684.1"
                     /translation="RADIVVVDTVGDLMGIYARSVAAFVGGSLAPYGGQNMLEPLFVG
                     TPVLFGPHTENFHDIAASILERKAGFLVETADDIHRAVMRLLTDNDLYESTRQAGFAI
                     VAGQKDVMDETARIIRGLLEPRSGREG"
     gene            394..1155
                     /gene="mazG"
                     /locus_tag="PKN85_09490"
     CDS             394..1155
                     /gene="mazG"
                     /locus_tag="PKN85_09490"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNT44685.1"
                     /translation="MESFDKLVWLMERLRSEEGCPWDRKQKVSAFKTFMLEEVYEIIE
                     AIEKDDMKDLKEELGDLLFHIVFIAQICRERDAFDINDVIENVYGKMYHRHPHVFRKE
                     VLDVPIEMKWEDLKREEKRDTGYSLLANIPPSMPALLRAYVMTKRAAKVGFDWQKIED
                     VYEKMYEEIAELKAAEAEGDPERVREEVGDLLFTIVNIARFLSVDPEDALRATSDKFT
                     RRFSYIENNTDIRNSDADTMDKLWNDSKSLEDKGK"
     gene            complement(1164..1367)
                     /locus_tag="PKN85_09495"
     CDS             complement(1164..1367)
                     /locus_tag="PKN85_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNT44686.1"
                     /translation="MPLGKVKWFNDSKGYGFIEQDGGEDVFVHFSAIQQDGFKSLKTG
                     EKVEFEIVKGPKGPQAANVMKAE"
     gene            complement(1507..>2028)
                     /locus_tag="PKN85_09500"
     CDS             complement(1507..>2028)
                     /locus_tag="PKN85_09500"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44687.1"
                     /translation="GFSRRYTVGVWVGNFSGEPMRDVSGISGAAPLWLEVMNFLHGTV
                     PSTAPARPRGVTVARVRFEGDIEPPREEFFLEGTEPMEVVRVQSAHEKPRIVYPPEGT
                     LITIDPDIPEKLQRVAMRFEPRSRRLRWVLNNTDAGSDTPLFLWEPRRGNYTLSIVDG
                     DGSIVDSVGFAVR"
BASE COUNT          467 a          589 c          588 g          384 t
ORIGIN      
        1 ccgcgccgac atcgtggtcg tcgacaccgt gggcgacctc atggggatct acgcgagaag
       61 cgtggccgcc ttcgtcggcg gttccctcgc tccctacggc ggtcagaaca tgctcgagcc
      121 cctcttcgtg ggcacacccg tgctgttcgg gccacacacg gaaaacttcc acgacattgc
      181 cgcctccatc cttgaaagaa aggcaggttt cctcgtggaa accgccgacg acatacaccg
      241 cgcggtgatg cggctcctca ccgataacga tctctacgag tccacacggc aggccggttt
      301 cgccatcgtc gccggtcaga aggacgttat ggacgagacg gcgcggatca taaggggcct
      361 cctcgagccc cggagcggga gagagggctg agaatggaat cctttgacaa actcgtatgg
      421 ctgatggaaa ggctcaggag cgaggaagga tgcccctggg accgcaaaca gaaggtttcc
      481 gccttcaaga ccttcatgct cgaagaggtc tacgagatca tcgaggccat agaaaaggac
      541 gacatgaagg atctcaagga agagctcggg gacctcctct tccacatcgt cttcatcgcg
      601 cagatatgca gggagcgcga tgccttcgac atcaatgacg ttatcgagaa cgtctacggc
      661 aagatgtacc accgtcatcc ccacgtcttc cggaaagagg tgctggacgt ccccatcgaa
      721 atgaagtggg aggacctgaa gagggaagag aagagggata ccgggtactc gctcctcgcg
      781 aacatccccc cgtccatgcc cgccctcctg agggcatacg tcatgacgaa gagggccgcg
      841 aaggtcgggt tcgactggca gaagatcgag gacgtgtacg aaaagatgta cgaggagatc
      901 gccgagctga aggcggcaga ggcggaaggc gatcccgagc gggtcagaga ggaggtgggt
      961 gacctgcttt tcaccatcgt caacatagcc cggttcctgt cggtggaccc tgaagacgcg
     1021 ctacgggcaa cctcggacaa gttcactcga aggttcagct acattgagaa caatacggat
     1081 atccggaatt ctgatgccga cacgatggac aaactctgga atgacagtaa atctttggag
     1141 gacaagggca aatagcccct gactcactcg gccttcatta catttgccgc ctgcggaccc
     1201 ttcggcccct tcacgatctc gaattcgacc ttctcaccgg tcttgagcga tttgaaacca
     1261 tcctgctgga tcgcggagaa gtgcacgaaa acatcctccc cgccgtcctg ttcgatgaaa
     1321 ccgtacccct tggaatcgtt gaaccatttc accttaccta gtggcatact gcatgactcc
     1381 tttctttggg aatgttaagc atttgtgctt ttaagcaata ttattcctaa taaaaatagc
     1441 tgtcaagcga aatctgtcgg tatcccttga tattatacgg gaaatcgcat tttggagggc
     1501 gtaagcctat ctgaccgcga accccaccga atcgacgatg ctgccatcgc cgtcgacgat
     1561 ggaaagggta taattgcccc gccggggctc ccagaggaag agcggtgtat cggaacccgc
     1621 gtcggtattg ttgaggaccc atctcaaacg cctcgaccgg ggctcgaagc gcatggcgac
     1681 ccgctggagc ttctcgggga tgtcgggatc gatggtgatg agcgtgcctt ccggagggta
     1741 gacgatgcgg ggtttctcgt gggccgactg cacacgcacg acctccatag gctcggtacc
     1801 ctcaaggaag aactcctcgc ggggaggctc gatatcccct tcgaagcgga ccctggcaac
     1861 ggtgacaccc cgcgggcgcg cgggcgctgt cgacggcacg gtgccatgaa gaaagttcat
     1921 cacctcgagc cacaggggcg ccgccccgct gataccgctg acatcacgca tcggctctcc
     1981 cgagaagttg ccgacccaca cgccaaccgt gtagcggcgg gagaaacc
//
LOCUS       DAOQTR010000431         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_71460_length_2017_cov_2.637615, whole genome shotgun sequence.
ACCESSION   DAOQTR010000431 DAOQTR010000000
VERSION     DAOQTR010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71460_length_2017_cov_2.637615"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..966
                     /locus_tag="PKN85_09505"
     CDS             199..966
                     /locus_tag="PKN85_09505"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNT44688.1"
                     /translation="MRVKILGCSGNLVGRHRTTSFLLDDSFLIDAGTVTEAIRRRDLR
                     KIRHVLVSHTHMDHVKGLFPLVDELVVMGDYSVELVSVAPVLDIIANNLLNNLIWPDF
                     TVIPSVSKAVIRLKEIPLERPSVLDRISVTPVLVTHTVYTVGFVVRQDDGRGFMFTAD
                     TGPTKRFWEVAGGEKDIGFIIADVSFPNRLADLATLSGHMTPSMLMESIDAHELGDRL
                     FYVSHMKPVFAREIIAEFERSGRKNIRFLRQSEILTV"
     gene            1155..>2017
                     /locus_tag="PKN85_09510"
     CDS             1155..>2017
                     /locus_tag="PKN85_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT44689.1"
                     /translation="MFDQGLKEFDQENYEEALPLLLEAWSADRGSARTAYYIGLTYKT
                     MEKYPEALPYFREAVSLTPRVDEGVVELVDVLYHTNNIVEAEKWIAFADREGISTPRL
                     HFLKGMVLAKRGRLDEAVLAFETAKKLDPGLTQQAELQIAGIYTQQGRYKDAQERLRT
                     TITLDPASDLALYARDYEKIVADKAERERPWRFSVGMGYKYDTNVVTKGDGPMVESIS
                     GQEDSAFNFGARIGYTAPFSFRTPFSFSAYYSVYSDRYFGKTYTRSDGSRGNLTEYNN
                     MTNTVSAVPGYA"
BASE COUNT          439 a          571 c          568 g          439 t
ORIGIN      
        1 actggtattc atagataggc atggtgttcc tccaacgcaa aaaaagtgtc taagtctcag
       61 taatataatg ctttaggcgt gtcgcgtcaa gtcataattg tgaacgatgt gcccgccgtc
      121 gctgtgaaac ggggtggttc cgccctcgtt tggcaagact tcttgacatg ccggagaacc
      181 gtcacataca atcagaagat gcgggtcaag atcctcggtt gctcaggcaa cctcgtcggc
      241 aggcacagga ccacatcttt tctgcttgac gattcctttc ttatcgacgc agggacggtt
      301 acggaggcga tacgccgcag ggacctgagg aaaatcaggc atgtccttgt ctcccacacg
      361 cacatggatc atgtcaaggg tcttttcccc ctggtggacg aactcgtcgt gatgggcgac
      421 tacagcgtgg aactggtcag tgttgccccc gtcctcgaca tcattgctaa taatcttctc
      481 aacaacctga tctggcccga ctttacggtc atcccctcgg tctcgaaggc ggtcatccgg
      541 ctcaaggaga tcccccttga gaggccatcc gtcctcgaca ggatcagcgt cacgcccgtg
      601 ctcgtgaccc atacggtcta cacggtgggt ttcgtggtca ggcaggacga cgggcggggt
      661 tttatgttca ccgcggacac cgggccgacg aaaaggttct gggaggtcgc aggaggggag
      721 aaggacatcg gtttcatcat cgccgatgtg tctttcccga accgccttgc agaccttgcg
      781 accctttcgg gccacatgac accctcgatg ctcatggaaa gcatcgatgc ccacgaactg
      841 ggcgacaggc tcttctacgt ctcccatatg aagccggtct ttgcccgtga gatcatcgcc
      901 gagttcgaac gctcgggcag gaagaacatc cgttttctga ggcagtcgga aatattgacc
      961 gtgtgagccc cccattgacc tgttttatac ccaactttct cttgtaaaga tggtttgtat
     1021 aggttaataa ttctttacat acgcatcatt atctgtggtg aatcgggggt ttgttaatgg
     1081 aacgagcacg cacgtcgtcc ctgttggcca tgtttttctt gatctcgacg gtctgccttg
     1141 tccacggaca ggcgttgttc gatcaaggct tgaaggagtt cgatcaggag aactacgaag
     1201 aggctctgcc ccttcttctt gaggcgtggt cggccgacag ggggtcggcc cggacggcat
     1261 attacatcgg cctcacgtac aagaccatgg agaagtaccc ggaggccctg ccctacttca
     1321 gggaggctgt ctcactgacc ccccgtgttg acgaaggggt tgtcgagctt gtcgatgtgc
     1381 tctaccacac caacaatatc gtcgaagcgg agaagtggat cgccttcgcg gacagggaag
     1441 ggatcagtac gccccgtctg cattttctca agggcatggt gctggccaag agggggaggc
     1501 tcgatgaagc cgttcttgcc ttcgaaacgg caaagaaact cgaccccgga ctgacgcagc
     1561 aggccgagct ccagatagcc ggcatctaca cgcaacaggg aagatacaag gacgcccagg
     1621 agcgcttaag gacgaccatc accctcgatc ccgcgagcga cctggccctc tatgccaggg
     1681 actacgagaa gatcgtcgcc gacaaggccg aacgggagag gccctggcgg ttcagcgtcg
     1741 ggatggggta caagtacgac accaacgtcg tcaccaaggg tgacggtcct atggtggagt
     1801 ccatatccgg gcaggaggac agcgccttca atttcggcgc ccgcatcggc tacaccgccc
     1861 ctttctcctt cagaacaccc ttcagcttct ccgcgtacta ctccgtgtac tcggacaggt
     1921 atttcgggaa gacctacacg cgctccgacg ggagccgggg caacctgacg gaatacaaca
     1981 acatgacgaa tacggtgagc gccgtccccg gttatgc
//
LOCUS       DAOQTR010000432         2016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_71497_length_2016_cov_4.234574, whole genome shotgun sequence.
ACCESSION   DAOQTR010000432 DAOQTR010000000
VERSION     DAOQTR010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71497_length_2016_cov_4.234574"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKN85_09515"
     CDS             <1..327
                     /locus_tag="PKN85_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44690.1"
                     /translation="GDRASYLSTGGSTIVLPRHMMEEKKDSYLRDLVGRQDIASLAPL
                     FRRLLEDYAYLFHCMGDGARYKGTLSILRDKTAFDHALSFFLRKSLETGQEGPSESDL
                     IINPYG"
     gene            320..946
                     /locus_tag="PKN85_09520"
     CDS             320..946
                     /locus_tag="PKN85_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNT44691.1"
                     /translation="MARFILKDPFYKKAKQEGFRARSAYKLKEIEDRFHLVHKGDAVL
                     DLGCSPGSFLQVLSGMVGEKGTVIGIDILPTPPLPPKNITTIEADIRETDVPGLLSSF
                     SMTAVDTVTCDIAPNLSGIREVDDAHIEDLFTCVLKVIEEVLKPGGHFVIKSFSTGIQ
                     KRITAELQMRFRKVSQYKPAASRTVSSEVFFVCMGKKTVARPKSQVSG"
     gene            complement(1046..>2016)
                     /locus_tag="PKN85_09525"
     CDS             complement(1046..>2016)
                     /locus_tag="PKN85_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT44692.1"
                     /translation="IIANIWQALSRIDVGTGDYFLNALPIFHSFGLTIGVFLPLFAGA
                     RSFLYVSPLHYRVVPEIAYEEGCTVLMGTNVFLNGYARRAHPYDFHTMRYVFCGAEAL
                     SEAVFEHYAKVFGIRVMSGYGATECAPIVCMNSALENRHGSVGRVLPGIEYKVMPVRG
                     IDSKEGHLGRLFVKGRNVMMGYLDNEAANHKYLVEDGGWYDTGDIVEIDDSGFVTIVG
                     RLKRFSKISGEMISLTAIEEALAGIFGERRELAVMAVTDERKGEKLVLVTNARAADLK
                     RVRDVLREKGHSDLTFPREIIYMKELPKLGTGKPDYVKLKEMLGSK"
BASE COUNT          419 a          629 c          550 g          418 t
ORIGIN      
        1 ggggaccgcg cgtcctacct ctcgactgga gggtccacga tcgtcctgcc ccggcacatg
       61 atggaagaaa agaaggattc ctacctcagg gacctcgtgg gacgccagga tatcgcgtcg
      121 ctggctcctc ttttcagacg cctcctcgag gactatgcct atctctttca ctgcatgggc
      181 gatggggcgc gatacaaggg cacgctctcg atccttcggg acaaaacagc cttcgaccat
      241 gccctctcct tctttttgag gaagagcctg gagaccgggc aggaaggccc ttccgaaagc
      301 gatctcataa tcaaccccta tggctaggtt catcctcaaa gacccattct acaagaaggc
      361 gaagcaggaa gggttccggg cgagaagcgc ctacaagctc aaggagatcg aggacaggtt
      421 ccatctcgtg cacaaagggg atgccgtcct ggacctcggc tgttcaccgg gaagctttct
      481 ccaggtgctt tccgggatgg tcggcgaaaa ggggacggtc atcggcatcg acatcctgcc
      541 cactccgcct ctccccccga agaacataac caccatagag gcggacatac gggaaaccga
      601 cgtccccggg cttctttcca gcttctctat gaccgccgtc gacaccgtta cctgcgacat
      661 cgcacccaac ctctcgggga tacgcgaggt ggacgacgcg cacatcgaag acctcttcac
      721 ctgtgtcctc aaggtcatcg aagaggtcct caaacccggc ggccacttcg taataaagtc
      781 cttttcaacg ggcattcaga aacgtatcac ggccgagctc cagatgcgct tccgcaaggt
      841 ctcccagtat aaacccgcag cctcacgaac cgtaagctcc gaggtgttct tcgtgtgcat
      901 ggggaagaaa accgtcgcac gtcccaagtc tcaggtctca ggctagaagc gtcgcagggg
      961 tcagggagca gcgagttttt tcggcctttt ttgcctttag accctgtgat ctgcgacgtg
     1021 cgacctgcga cgggaggccc cagcctcact tgcttcccag catctccttg agcttcacat
     1081 aatccggctt gcccgtgccc agtttcggca gttccttcat gtaaatgatc tcacggggaa
     1141 aggtcaggtc ggaatggcct ttttcgcgca ggacatcgcg caccctcttc aggtcggcgg
     1201 ccctggcatt cgtcacgagg acaagcttct ctcccttgcg ctcgtcggtt acggccatga
     1261 cggcgagttc ccttcgctcg ccgaatatcc ccgcaagggc ctcttcgatg gcagtgaggg
     1321 atatcatctc gccgctgatc ttggagaagc gtttcaatcg ccccacgatg gtcacgaaac
     1381 cgctgtcgtc gatctccact atgtcgcccg tatcgtacca gccgccatcc tccacgagat
     1441 atttatggtt cgcggcctca ttgtccagat atcccatcat gacgttccgg cccttcacga
     1501 agaggcggcc caggtgcccc tccttcgagt caatgcccct aacgggcatc accttgtatt
     1561 caatgccggg aagcaccctg ccgacactgc cgtgcctgtt ctcgagggca ctgttcatgc
     1621 acacgatggg tgcgcattcc gtggcgccat acccggacat cacccgtata ccgaagacct
     1681 tagcgtagtg ctcgaagacg gcttcgctca gcgcttccgc cccacagaac acgtagcgca
     1741 tggtgtggaa atcgtagggg tgcgcccttc gggcgtagcc gttcaggaag acgttcgtac
     1801 ccatgaggac agtgcagcct tcctcgtatg cgatctcggg caccaccctg tagtgcagcg
     1861 gactcacata caggaaggaa cgggcgccgg caaacagggg caggaaaacc ccgatggtca
     1921 ggccaaaact gtggaatatg ggtagggcat tgaggaagta gtccccggtc cccacgtcga
     1981 tgcgggatag ggcctgccat atgttggcta tgatgt
//
LOCUS       DAOQTR010000433         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_72213_length_2004_cov_4.005644, whole genome shotgun sequence.
ACCESSION   DAOQTR010000433 DAOQTR010000000
VERSION     DAOQTR010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72213_length_2004_cov_4.005644"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..905)
                     /locus_tag="PKN85_09530"
     CDS             complement(171..905)
                     /locus_tag="PKN85_09530"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNT44693.1"
                     /translation="MGGIYGARTLLCVKLDHVAELRQARSSDGFDPAVYAATCDRKGC
                     KGVSAHLGRDRRYMSVEDVHGIKDAIRGEFNLEIGLSDEMIGLAREVRPHIVTIVPEM
                     KEETTPGGGFDVKTHLSGIRDAVRLLHDDGIQVSFLIDPDIEALEYSKECAADRVEIC
                     MAGYCDARRKDEIDKEIRKVCAAVNHAAGMRLRVTAGRKLDYTNIEPLLDAAGLTEVH
                     IGRAIISRSVEVGLSRALDDMLEILE"
     gene            complement(1018..>2004)
                     /gene="arsB"
                     /locus_tag="PKN85_09535"
     CDS             complement(1018..>2004)
                     /gene="arsB"
                     /locus_tag="PKN85_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNT44694.1"
                     /translation="GVVDFWNRFQSGTTNIPIAMGLILMMYPPLAKVKYEELGEVFKN
                     WRVLVLSLIQNWVVGPILMFILAIAFLRGYPEYMVGLIMIGLARCIAMVIVWNDLAKG
                     DTEYCAGLVAFNSIFQVLFFSVYAYIFITVLPPLMGLKGVVVDITIGQIAESVFIYLG
                     IPFIAGVVTRFILIKVKDKTWYHERFIPKISPITLIALLFTILVMFSLKGEYIVKIPM
                     DVVRIAIPLLIYFVFMFVVSFYMSAKARAHYGQAATLSFTAASNNFELAIAVAVAVFG
                     INSGQAFAAVIGPLVEVPVLINLVHLSLWIKKRYFPYAVETPTGVCYMSCKE"
BASE COUNT          468 a          582 c          528 g          426 t
ORIGIN      
        1 aaccgcggga cccttcgacg acgcggaggc ctttcgacct gccatggcct cacccgcggc
       61 aacagcggtc gatgccatga tggccgacga cagcatggtg aggatgatca tacagaatat
      121 catggatcta ttcttcattc cgaactcctt cctgttggtc aaggtaatgg ttactccaga
      181 atttccagca tatcgtcgag tgcccgtgaa agacccactt caacggacct cgatatgatg
      241 gcacggccga tatgcacctc cgtcagcccg gcagcatcga gaaggggctc gatgttcgta
      301 taatcgagct tccggccggc cgtgacgcgc aacctcatcc ctgcggcatg attgacggcg
      361 gcgcagacct tccttatctc tttgtcaatc tcgtccttcc gccgggcatc acagtatccc
      421 gccatacata tctccacgcg gtccgcggca cattccttcg agtactccag cgcttcgatg
      481 tccgggtcga tgaggaagga cacctgaatc ccgtcgtcgt ggagaagcct gacggcatcc
      541 cgtatgccgg acagatgtgt cttcacgtcg aacccgcctc ccggggtcgt ttcttccttc
      601 atctccggca cgatcgtgac aatatgaggt ctcacttccc ttgcaaggcc gatcatctca
      661 tccgacaggc cgatctcaag gttaaactct ccccggatgg cgtcctttat cccatggacg
      721 tcttccacac tcatgtagcg cctgtccctg ccaaggtggg cggagacacc cttgcaaccc
      781 ttcctgtcgc aggttgccgc ataaacggca gggtcaaatc catctgacga gcgcgcctga
      841 cgcaattcgg caacatggtc cagtttcacg cacagcaggg tgcgcgctcc atagattcct
      901 cccattggca tcaaactcct ccttccccgt tctctcaacc aacggtacgg ggggtagtcc
      961 tgaaccggta tgtccggccg gtccaacgac cggcaaaacg atatgtgtgg tttccgttca
     1021 ttccttgcat gacatataac aaacacccgt gggcgtttcc accgcatagg ggaagtatct
     1081 cttcttgatc cacagcgaca ggtgaacgag attgatcagc accggcacct cgacaagagg
     1141 tccgatgacg gcggcgaagg cctgtcccga gttgatgccg aaaacggcaa cggcaaccgc
     1201 gatcgcaagc tcgaagttgt tcgacgcggc agtgaaagac agcgtggctg cctggccgta
     1261 atgcgctctc gccttcgcgc tcatgtagaa agaaacaacg aacatgaaca caaagtagat
     1321 aagaaggggt atggcgatgc gcaccacgtc catggggatc ttcacgatgt attcaccctt
     1381 cagggagaac atgacaagta tggtgaaaag caaggcgatg agcgttatgg ggcttatctt
     1441 cggtatgaac ctctcgtggt accatgtctt gtccttgacc ttgataagga taaagcgcgt
     1501 gacaaccccc gcgatgaagg gtatgcccag gtagatgaaa acgctttccg ctatctggcc
     1561 gatggtgata tcgacaacca cgcctttgag tcccataagg gggggcagga cggttatgaa
     1621 gatatacgca tacacagaga agaaaaggac ctgaaagatc gaattgaagg cgacaagacc
     1681 ggcgcagtac tcggtgtcgc cctttgccag gtcgttccag actattacca tggctatgca
     1741 ccgggcaagg ccgatcatga taaggccgac catgtattcg gggtaacctc tcagaaatgc
     1801 gatggcaaga atgaacatca gtatgggccc caccacccag ttctggataa gggagagaac
     1861 gagaaccctc cagttcttga agacctcccc cagttcttcg tatttcacct tcgccagcgg
     1921 cgggtacatc atgaggataa ggcccatggc tatggggatg ttggtggtcc cgctctggaa
     1981 ccggttccag aaatcgacaa cccc
//
LOCUS       DAOQTR010000434         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_72322_length_2002_cov_3.574217, whole genome shotgun sequence.
ACCESSION   DAOQTR010000434 DAOQTR010000000
VERSION     DAOQTR010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72322_length_2002_cov_3.574217"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..438
                     /locus_tag="PKN85_09540"
     CDS             112..438
                     /locus_tag="PKN85_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44695.1"
                     /translation="MKKAAIFIMIAAFSCGLFVLPAFAEDENERSWRGSGKGEAMFFD
                     LVLLRPIGIASVGVGFAATIVALPFSIIANNTREVGDAMLTETVNYTFVRPLGEVFEP
                     ASMLDR"
     gene            619..>2002
                     /gene="glgP"
                     /locus_tag="PKN85_09545"
     CDS             619..>2002
                     /gene="glgP"
                     /locus_tag="PKN85_09545"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HNT44696.1"
                     /translation="MSQTSDGAGATRLDGRSGDDFRRILTNQLTYSLSKDMYSATQRD
                     KYTATALSVRARIVKNWIECQQSYYKHDAKRLYYLSLEFLMGRLLKNYLINIDLLDEY
                     REAMDVLATRLEDTLEYEWDAGLGNGGLGRLAACFMDSMATLNYPAYGYGIRYEYGIF
                     TQRIVDGYQTEAPDNWLRYGNPWEFPRPELIYPVRFYGRVETHSARTEWVDTSEILAM
                     AYDYPVPGFRTNTVNTLRLWAAKSTRDFDFEYFNSGDYVRAVENKNSSENISKVLYPN
                     DLSLAGKELRLKQQYFFVAATLADIMRRFGKAYSDLSMLPAKVAIQLNDTHPSIAIPE
                     MMRILIDDHGLSWDIASKLTRETFAYTNHTILPEALETWSEDLIGHLLPRHLQIIKEL
                     DRKFHIQVARRFPEEFDKAYQMGIVTGDGGKVVNLARLAIVGSHAVNGVSRLHSELLK
                     SHVFNDFYLMY"
BASE COUNT          459 a          559 c          568 g          416 t
ORIGIN      
        1 tccaacgttc aagctgttca agccgcttta gatatgtaca caaaaaaatt attgaaaacg
       61 gtaaaatgct gtgctataaa aacaaaaggt cttaagaaaa gggggtaaca tatgaaaaaa
      121 gctgcaatat ttattatgat cgctgccttt tcgtgtggtc ttttcgtgct tcccgctttt
      181 gccgaggatg agaacgagcg ttcctggcgg gggtccggca agggggaggc catgttcttc
      241 gaccttgttc tcttgagacc tatcggcatt gccagcgtgg gagtcggttt cgcggcaacc
      301 atcgtggccc tgcctttttc cataatagcg aacaacacga gggaggtcgg ggatgccatg
      361 ctgaccgaga ccgtcaacta taccttcgtg aggcctctcg gcgaggtctt tgagccggca
      421 tcgatgttgg atcgctagga tggtcgcccc gggcaacact gaagatggtg tcctggggcc
      481 ggggaccgct ggaagggcga catcccgccg tgttcgatca cggtcattcc ttaccaccac
      541 cgctaaagaa tctttagtaa atatcgataa ctataggtat cggagcttcg gctccgtcat
      601 tatcaaagga gatgtcacat gagtcaaacc tctgatggcg ccggcgcgac acggttagat
      661 ggccggtccg gggatgattt tcgcaggatc ctgacgaacc agctcaccta ttctctttcg
      721 aaagacatgt attcggcgac gcaaagggac aagtatacgg ccacggccct ttcggtcagg
      781 gcccgcatag tcaagaactg gatcgaatgc cagcagagct attacaagca cgatgccaag
      841 cgcctctact acctttccct ggagttcctc atggggaggc ttctcaagaa ctacctcatc
      901 aacatcgacc ttctcgacga ataccgcgag gccatggacg tgttggccac caggctggag
      961 gacaccctgg agtacgagtg ggacgcggga ctcggcaatg ggggcctcgg caggctcgcg
     1021 gcctgtttca tggattccat ggccacgctg aactacccgg cctatggcta cggcatcagg
     1081 tacgagtatg ggatcttcac gcagaggatc gtcgacgggt atcagacgga agcccccgac
     1141 aactggctgc ggtatggcaa cccttgggag ttccctcgac ctgagctcat atatcccgtc
     1201 cggttctacg ggagggtgga gacgcactcc gccaggacgg aatgggtcga cacgagcgag
     1261 atcctggcca tggcctacga ttaccccgtg cccgggttcc gcacgaacac ggtgaacacc
     1321 ctgcgcctct gggcggcgaa atcgacgcgt gattttgatt tcgaatactt caacagcggc
     1381 gattatgtca gggcagtgga gaacaagaac agcagcgaga acatctcgaa ggtcctttac
     1441 cccaacgacc tgtcgctggc aggcaaggag ctgaggctca aacagcagta cttcttcgtg
     1501 gctgccaccc ttgccgacat catgaggcgg ttcgggaagg cgtacagcga tctcagcatg
     1561 ctgccggcca aggtcgcgat ccagctcaac gacacccacc cctcgatcgc gatccccgaa
     1621 atgatgagga tactcatcga cgatcacgga ctctcctggg atattgcgag caagctcacg
     1681 cgggagacct ttgcctatac gaaccacacc atccttcccg aggcgctcga aacctggtcg
     1741 gaggacctca tcgggcacct gcttccccgc cacctccaga tcataaagga gttggacagg
     1801 aagttccata tccaggtggc ccggcgcttt cccgaggagt tcgacaaagc ctaccagatg
     1861 gggatcgtca cgggagatgg agggaaggtg gtcaatttgg cccggctcgc catcgtggga
     1921 agccacgcgg tgaacggggt gtcccgtctg cacagcgagc tcctgaagag ccacgtgttc
     1981 aacgatttct acctcatgta cc
//
LOCUS       DAOQTR010000435         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_72470_length_1999_cov_3.948560, whole genome shotgun sequence.
ACCESSION   DAOQTR010000435 DAOQTR010000000
VERSION     DAOQTR010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72470_length_1999_cov_3.948560"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PKN85_09550"
     CDS             complement(<1..712)
                     /locus_tag="PKN85_09550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNT44697.1"
                     /translation="MEAADTRMSPREVLKRYFGYTSFRTHQEEIVEHLMAGGDAFVLM
                     PTGSGKSLCYQVPALIRRGVCVVVSPLIALMQDQVDAMRELGIKAGFLNSTLDSREAR
                     AIERRAMEGGIDLLYVAPERLVTDGFMALLSRMEIALFAIDEAHCVSQWGHDFRPEYL
                     ELGVLAGKFPGVPRVTLTATADETTRREIIDKLRLAGARRFITSFDRPNIRYRVEIKE
                     KEKDQLLDFIRSEHPGQPG"
     gene            complement(952..1566)
                     /locus_tag="PKN85_09555"
     CDS             complement(952..1566)
                     /locus_tag="PKN85_09555"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNT44698.1"
                     /translation="MEAQKLYALPDLPYGYDALAPYISKELLTLHHDKHHAAYVNGAN
                     AILQRLDKAKTDNADFDTKATYKELSFHIGGHLLHYIFWENLAPAGKGGGGKPTGALA
                     ELLDREYGSFDRFKKIFSQAAMSVEGSGWATLTFCKLTNRPLIMQVEKHNVHIYPMFR
                     ILMALDVWEHAYYLDYKNDRAKFIEAYWNIVNWGAASKSLEEVL"
     gene            1676..1822
                     /locus_tag="PKN85_09560"
     CDS             1676..1822
                     /locus_tag="PKN85_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44699.1"
                     /translation="MMISSPRTALPYKELPSFLEKIWHSIPELENTSLDGMTRDLNPI
                     ALHP"
BASE COUNT          419 a          582 c          537 g          461 t
ORIGIN      
        1 tgcccggctg cccggggtgc tctgatctga tgaagtcgag aagctggtcc ttttccttct
       61 ccttgatctc cactctgtaa cgtatgttgg gcctgtcgaa gcttgtgata aagcgcctcg
      121 ccccggccag gcgcagtttg tcgatgattt cccgtctcgt ggtctcgtcg gccgtcgcgg
      181 tgagcgtcac acgcggcacc ccggggaatt ttcccgccag gacgcccagt tccaggtact
      241 cgggccggaa atcgtgtccc cactgggaga cgcagtgagc ctcgtctatc gcgaagaggg
      301 ctatctccat tctgctgaga agggccatga aaccgtcggt gacaagcctc tcgggtgcca
      361 cgtagaggag atcgataccg ccttccattg cgcgcctttc aattgcgcgg gcctcgcgcg
      421 agtcgagggt ggagttgaga aagcccgcct tgatgcccag ttcccgcata gcgtccacct
      481 gatcctgcat gagcgctatg aggggggaga ccacgacgca cacaccccgc cggatcaggg
      541 ccgggacctg gtagcacaat gattttccac tgcctgtggg catgagcacg aaggcatccc
      601 ccccggccat gagatgctcg acgatctctt cctggtgagt gcgaaaggac gtgtatccga
      661 aatatctctt cagaacctcc cgcggcgaca tgcgtgtatc tgcggcttcc atagacaacc
      721 ctcgctcgac gaaatatgcc cgccactaga ttaccccaac gggccgctta aaaccacacc
      781 ggagatatat tactttttag aggagaaaaa aacgaggtga caggaataat aatcacgaaa
      841 cacatcaacc ggctcaagac cgaaacccag gctttctgcg tttccacatc cctcctggac
      901 tccggcccaa agccggaatg acgaacaacg ttcaagcggc tcaagccgtc ttcacagcac
      961 ttcctccagg ctcttgctcg cggctcccca gttgacgatg ttccagtatg cctcgatgaa
     1021 cttcgccctg tcgttcttgt agtccaggta gtaggcatgt tcccagacat cgagggccat
     1081 gaggatcctg aacatgggat agatgtgaac gttgtgtttc tccacctgca tgatcagcgg
     1141 tctgttcgtc agtttgcaga aggtgagcgt tgcccagccc gagccctcca cgctcatggc
     1201 cgcctgggaa aagatcttct taaaacggtc gaaagagccg tattccctgt cgaggagttc
     1261 ggcgagggcc cccgtcggtt tgcctccgcc gcccttgcca gcgggtgcca ggttctccca
     1321 gaagatataa tggaggagat gccccccgat gtggaaggac agttctttat aggtcgcttt
     1381 cgtgtcaaag tccgcgttgt cggttttcgc cttgtcgagt ctctgcagga tggcattggc
     1441 cccgtttacg taggccgcgt ggtgcttgtc gtgatgcagc gtgagaagtt ctttggatat
     1501 gtatggcgca agcgcgtcat aaccataggg gagatcgggt aaagcgtaga gtttctgtgc
     1561 ttccatttcg ttacctcctt tgaattcttg ttgcgggttt acccgtctgt tcctgactcg
     1621 agtgaagccg gccgcatata tcaattgtac cacgtaattt gccgggagcc gaacgatgat
     1681 gatctcctcc ccgcgtacgg ccttgcccta caaggaactc ccctcttttc ttgaaaaaat
     1741 ctggcactcc attccggagc ttgagaacac ctctctcgac ggtatgaccc gggacttgaa
     1801 ccccatagcc ctgcacccgt aagggaacga cttgtcatag gtaatataga aatgaacaca
     1861 ggaatgacac ttgactgttc ctgcaacacc ctgccgtcca ccgtgagagt ctgttttccc
     1921 gttcatgcaa cggtccttcc gctgggatga caaggacaag gaacgctcaa gcggctcaag
     1981 cgattcaagc tgttcaagc
//
LOCUS       DAOQTR010000436         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_72544_length_1998_cov_3.218734, whole genome shotgun sequence.
ACCESSION   DAOQTR010000436 DAOQTR010000000
VERSION     DAOQTR010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72544_length_1998_cov_3.218734"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /gene="argC"
                     /locus_tag="PKN85_09565"
     CDS             <1..958
                     /gene="argC"
                     /locus_tag="PKN85_09565"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNT44700.1"
                     /translation="THPDAKIKLITSNTYRGRKISDIFPLFRGRLEDVLQPTDEDHRG
                     SCDVYFLCLPHGTAMDKAQELFDGKALIVDLSADFRFEDLAIYEGAYVPHTAKELAAQ
                     AVFGLPELYREMIKGAKLIGNPGCYPTSAILPLYPLVKHGMLEGDIFIDSKSGVSGAG
                     REGKVGSLFCEVSEGFRAYNVGVHRHEPEIQKEVNKYSPQRISFVPHLVPMSRGILTT
                     VYGRLAKAANTAEVLDVFRETFKGEPFVRIMDENVYPDTRFVRFSNYCDIGLKVLDDG
                     RIIVISTIDNLVKGASGQALQNMNVALGLPETAGLSGVPQYP"
     gene            complement(1115..>1998)
                     /locus_tag="PKN85_09570"
     CDS             complement(1115..>1998)
                     /locus_tag="PKN85_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNT44701.1"
                     /translation="RQLAAAIARADLAYAFLPVCCIFLCLTLCSFRWSMITGNAVRFR
                     DAFIALVIGLFINNVLPARIGEVARGYAISKRRGIPFTYAVSTVFIDRVFDLLGLLSI
                     TFLFFPRQALPPSVSKALYVLVAIFVVCVIVLVAVSRERTARKMARFLEGFKRPFLLR
                     LATRVLEIQENLRRISTPGQIALFIVLSVANWLSMSSALYFSLKTLGVPLPFVYAPFV
                     TALLNMGLAVPSSPGYIGVYQFLLVYLLAIFGVPKAQSFAVSLFFHASWYIPYNIMGF
                     IFILKEQVHIKDIRKAA"
BASE COUNT          453 a          560 c          620 g          365 t
ORIGIN      
        1 tactcatccc gatgcgaaga taaagctcat cacatccaac acgtatcggg gccgcaagat
       61 atcggatatc tttccccttt tccggggccg gctggaggac gttcttcagc cgacggacga
      121 agaccatcgc ggttcctgcg acgtctattt cctgtgtctc ccccacggga ccgccatgga
      181 caaggcccag gaattgttcg acggaaaggc cctcatcgtg gacctcagcg ccgacttccg
      241 tttcgaggac ctggcgatct acgaaggcgc ctacgtgccc cacacggcga aggaactagc
      301 cgcccaggcg gtcttcggtc tccccgagct ctacagggag atgataaagg gagcgaagct
      361 catcggcaat ccgggttgct atccgacgtc cgccattctt cccctgtatc ctctcgtcaa
      421 gcacggaatg ctcgaaggcg atatcttcat cgactcaaag tcgggggtca gcggagccgg
      481 ccgggaggga aaggtcggca gtctcttctg cgaggtctcc gagggattcc gcgcctacaa
      541 cgtcggggtc caccggcacg agccggagat acagaaggaa gtcaacaagt actcacccca
      601 gaggatatcc tttgtccccc atctcgttcc catgtcgagg ggtatcctga cgacggtcta
      661 cggtagactg gccaaggcgg caaataccgc cgaggtcctc gacgtctttc gggagacctt
      721 caagggcgag ccttttgtcc ggatcatgga cgagaacgtt taccccgaca cccggttcgt
      781 caggttctcc aattactgcg acatcggtct caaggtcctc gacgacggcc gcatcatcgt
      841 catctccacc atcgacaacc tcgtcaaggg tgcctcgggc caggccctcc agaacatgaa
      901 cgtggccctc ggcctgcccg aaacagcagg gctttccggg gtaccccaat acccctaaag
      961 gcggcttgag tcgcttgaat agcttgaatc tattgagggt taaaaacctc aaggtatttg
     1021 acggctaatt ctccggcaaa agccgggacc acagttttct gcaacccaac cacccggagc
     1081 tttcttcttt tcactctcaa gctattcaag cgactcaagc cgcctttcgt atgtccttta
     1141 tatgtacctg ttccttgaga atgaagatga agcccatgat gttgtaaggg atgtaccagg
     1201 aggcgtggaa gaagagggat acggcgaagg attgcgcctt ggggacgccg aagatggcga
     1261 ggaggtagac gagaaggaac tggtagacgc cgatgtatcc cggggaagag ggcacggcga
     1321 ggcccatgtt gaggagggcc gtgacaaagg gggcgtagac gaagggaagg gggacgccga
     1381 gggtcttgag ggagaagtac agggccgagc tcatggagag ccagttggcg acggacagga
     1441 cgatgaagag cgcgatctgg cccggcgtgc tgatgcggcg gaggttctcc tgtatctcga
     1501 gcaccctcgt ggcaaggcgc aagaggaagg ggcgtttgaa tccttcgagg aaccgggcca
     1561 tcttgcgggc cgtcctctcc cggctcacgg caacgagcac tatgacgcat accacgaaga
     1621 tggcaaccag gacgtagagg gccttggaca ccgacggggg gagggcctgg cgggggaaga
     1681 agaggaaggt gatggacaaa aggcccagca ggtcaaagac cctgtcgatg aacaccgtcg
     1741 acaccgcgta cgtgaaaggg atgccccttc gcttcgagat ggcgtagccg cgtgcgacct
     1801 ccccgattcg ggcgggaagg acgttgttga tgaaaaggcc gatcacgagg gcgatgaatg
     1861 cgtcgcggaa cctgaccgcg ttccccgtta tcatggacca tctgaaagag cacagggtaa
     1921 ggcaaaggaa gatgcaacat acgggcagga aggcgtacgc aaggtcggcc ctggcgatcg
     1981 ccgcggcgag ctgcctgt
//
LOCUS       DAOQTR010000437         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_72597_length_1997_cov_3.488671, whole genome shotgun sequence.
ACCESSION   DAOQTR010000437 DAOQTR010000000
VERSION     DAOQTR010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72597_length_1997_cov_3.488671"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKN85_09575"
     CDS             <1..464
                     /locus_tag="PKN85_09575"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNT44702.1"
                     /translation="FSMVNFKPLWIAIALNKVHYSNGGIKESGAFSVNIPSADMVVVT
                     DYCGIVSGRKYDKSKEFETFYGESGDAPMIKECPYNIECRLINVVDLPSNELFIGEVL
                     TVYSDERYLTEGVPDMKKVNPLVLSMPERSYLTLGGRVAAAWETGGRLVRK"
     gene            461..1087
                     /locus_tag="PKN85_09580"
     CDS             461..1087
                     /locus_tag="PKN85_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44703.1"
                     /translation="MMEGAERSLDYVPEDRRVAESLGWQPGIWASFFLLVGCIILAES
                     KSPPDLVMMEILGVVFFAGLVLALWQAVMHRNRTVLVRQDGMIQVFRKRRLDMVVAAQ
                     EIRLEKVDIVAIIKIGAPLAIAAALFLALGLAMLWKDRAANSGSLSALALGLVSTASL
                     ASAFWTRFFRRHLLVPVRNSRWLAEETVLLSVKQHKLLFSGEDGGRPL"
     gene            1151..1393
                     /locus_tag="PKN85_09585"
     CDS             1151..1393
                     /locus_tag="PKN85_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44704.1"
                     /translation="MVEEHERRVPAGGISGTRMGSRMGKAKKIVSAARAFRVVTTLVI
                     VVVPVAATVGAIVGYGAYSLVKQLKRRSSAVRPREG"
     gene            1474..1869
                     /locus_tag="PKN85_09590"
     CDS             1474..1869
                     /locus_tag="PKN85_09590"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNT44705.1"
                     /translation="MAGVLVVGFLFIAVPAVVVVAVVWLLRKNQAGSRRLKAIPAGWR
                     PIEGRVIGNKVEEAARTNPGDWSFYFPMVFFEYTVEGQRYTGAQGVDRAYQEEYRVKK
                     VLADYPVGNPITVYYDPLKPGEAKLRMRL"
BASE COUNT          394 a          535 c          665 g          403 t
ORIGIN      
        1 ggttcagcat ggtcaatttc aagccgcttt ggatcgcgat cgcgttgaac aaggtccatt
       61 acagcaatgg cgggatcaag gagagcgggg ctttcagcgt caatatcccc tctgccgaca
      121 tggtggtcgt gacggattat tgcggcatcg tgtcggggag gaaatacgac aagtcgaagg
      181 agttcgagac gttctacggg gagtcaggag acgcgcccat gataaaggaa tgcccgtaca
      241 acatcgaatg ccggctcatc aatgtcgttg acctgccgtc gaacgaactg ttcatcggcg
      301 aggtgctgac cgtgtacagt gacgagcggt acctgacgga gggggtaccg gacatgaaga
      361 aggtgaatcc ccttgtcctg tccatgcccg agcggagcta tctcaccctg ggagggcgcg
      421 tcgcagccgc gtgggagaca ggcggacggt tggtaaggaa atgatggaag gggcggagcg
      481 atcactcgac tatgtccctg aagaccgccg agtggcggaa agcctcggtt ggcagccggg
      541 catctgggcc tctttctttc tccttgtcgg atgcatcatt ctcgcggaat ccaagtcgcc
      601 tccggacctc gtgatgatgg agatcctggg ggttgttttc ttcgccgggc tcgtgctggc
      661 cctctggcag gcggtcatgc acaggaaccg caccgttctt gtgaggcagg acggcatgat
      721 ccaggtcttc cgcaagagac gtctcgatat ggtcgtggcc gcacaggaga tccggctcga
      781 aaaggtggac atcgtcgcga taatcaagat cggcgcgccc ctcgccatcg ccgccgctct
      841 gttcctggcg ctcgggctcg cgatgctctg gaaggacagg gcggcgaatt ctggcagctt
      901 gagcgccctc gcccttgggc tcgtttcgac tgcttcgctg gcaagcgctt tctggacaag
      961 gttcttccga cgccacctgc tggtgcccgt caggaactcc cggtggcttg ccgaggaaac
     1021 cgtgcttctc tctgtcaaac aacataagct cctcttttcg ggagaagacg ggggcaggcc
     1081 cttgtgatga tcgccgggtc ttgctgccgg ttcctgatat tgcttgcaaa tcgggggaga
     1141 ttgcaggaaa atggttgaag aacacgaaag acgggttccg gcagggggaa tatcgggaac
     1201 gcgcatgggg tcgaggatgg gaaaggcgaa gaaaattgtc agcgccgcgc gggcgttcag
     1261 ggtggtgacc accctcgtca tcgttgtcgt ccccgtggcg gccaccgtag gggccatagt
     1321 cggctatggc gcgtactctc tggtgaagca gctgaaacga agatcgtccg cggttcgtcc
     1381 ccgtgagggg tgaacgagga cggatcacaa acgtcacccg gatcttcccg caatacacag
     1441 gcatcggtcg gtgaccaatt cgcaggaggg cttatggccg gggttttggt ggtgggattc
     1501 cttttcatcg cagtgcctgc cgttgtcgtg gtcgccgtgg tctggctctt gcgcaagaac
     1561 caggcgggga gccggaggct gaaggccatt ccggcgggct ggcgccctat agagggcagg
     1621 gtgataggga acaaggtgga ggaggccgcc cgtaccaacc ccggggattg gagcttttac
     1681 ttccccatgg tcttcttcga gtataccgtg gaggggcaga ggtacaccgg cgcccagggt
     1741 gttgaccggg cataccagga ggagtacagg gtaaagaagg tccttgcgga ttatcccgtg
     1801 ggaaacccca tcacggtcta ctacgacccg ctgaaacccg gggaggccaa gctgcggatg
     1861 aggttatgag gctccggcgc gcctttaggt ctcccaccca ctccccgatc gataccggag
     1921 gtcagttatg ttcgcgatag ctgtgaacgg cagcccccga aagggcggca acacggagat
     1981 actcttgaag gaagtgc
//
LOCUS       DAOQTR010000438         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_72675_length_1996_cov_2.624936, whole genome shotgun sequence.
ACCESSION   DAOQTR010000438 DAOQTR010000000
VERSION     DAOQTR010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72675_length_1996_cov_2.624936"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..752)
                     /locus_tag="PKN85_09595"
     CDS             complement(270..752)
                     /locus_tag="PKN85_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44706.1"
                     /translation="MDLIASGDLKGILDVLSFMADVERALAELYHACGETWDDGHGFW
                     SGIEDEENKHARNIESMKDIISRKPERFEKGRPFNIMAAQTLIRGIRGNIDKVRTGTI
                     QRKNALFMARDMEQSMMEQRYGEVVKTEDIEYLGLMKEIISDTAAHKAKLDAEIRNLN
                     "
     gene            968..>1996
                     /locus_tag="PKN85_09600"
     CDS             968..>1996
                     /locus_tag="PKN85_09600"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNT44707.1"
                     /translation="MPYDATKMADWQISEAAEEKMKSVRQLSDEMGLGDNEVIPYGKL
                     GRLNYAAIMQRLREKSNGKYIDVTAITPTPLGEGKTTTTIGLLEGLGKRGRNVGGAIR
                     QPSTGPTMNVKGSAGGGGNAQVVPLTEFSLGLTGDIDRIMNAHNIAMVALTARMQHER
                     NYGDGELAKRGLARLDVDPTNIQMGWVIDFCAQALRNIVVGMGGRMDGFTMQSHFDIG
                     VSSELMAILSIVRDLKDLRKRIGEITVAYRKNGEPVTTEDLEVAGAMCAIMKDTINPT
                     LMQTVEGQPIMVHAGPFANIAIGQSSIIVGVPQTFPILRPPRGCQITCFLTPSTKSAY
                     THPPELAQE"
BASE COUNT          433 a          584 c          597 g          382 t
ORIGIN      
        1 gtctccggga cagacccgga aggcgatcgg agaactccgg acaaaatgcc ggatctatgc
       61 gggcggtgcc atgaaaagag gcaagaagga agaaggggag cgctatctca gactggcatc
      121 ggcatatgaa gacgaagcga cccaggggga atagatgaac gatcgtatcg tcctctcggg
      181 ctatcccgcg aggtgtctcc gtgtgcgaac gaagcaccgg acccgtcgct ccgttgacgc
      241 gaagcgcccg cctgcttgga gcacagcttt cagttcaggt tcctgatctc ggcgtccaat
      301 ttggccttgt gcgcggcggt atcggagatt atctccttca tgagaccgag gtactcgatg
      361 tcttcggtct tgacgacctc accgtagcgc tgttccatca tggactgttc catgtcacgg
      421 gccatgaaga gggcgttctt tctctggatg gtgccggtcc tcaccttgtc gatgttcccc
      481 ctgatccctc tgatgagggt ctgggcagcc attatattga agggacggcc tttctcgaag
      541 cgttccggtt ttcgggatat gatgtccttc atgctctcga tattgcgtgc gtgcttgttc
      601 tcttcatcct cgatgccgga ccagaaacca tgcccgtcgt cccaggtctc tccgcaggca
      661 tgataaagct cggcgagcgc cctttccacg tctgccataa aggacaggac atcgaggata
      721 cctttcaggt ctcccgatgc tatcagatcc atatccacct cgtcatatcg ccgggtgagt
      781 tccccgaaac cttctgaggc atcattacac cgtgatgccg ccctaaaaac tatcagaagc
      841 ggaggacata ttcaatccga aagacgcgac atcgcgtcgc cgggcttgag aaatcgtgcc
      901 gaaacctgta tatttatcaa tgctttgagg cgaagttcaa gcctctctca tgaaaaggag
      961 cctgcatatg ccctatgacg cgacaaagat ggcggactgg cagatatccg aggccgccga
     1021 ggagaagatg aagagtgtcc ggcaactcag cgatgagatg ggtcttgggg acaacgaggt
     1081 cataccttac gggaagctgg gccgcctcaa ttatgccgcg atcatgcagc gcctgcggga
     1141 gaagtcgaac gggaagtaca tagacgtcac ggccatcacc cccacgccgc tcggtgaagg
     1201 aaagacgacg acaacgatcg gccttctcga gggactgggc aagagaggca ggaacgtggg
     1261 aggggccatt cgccagccgt cgacgggtcc gacgatgaac gtcaagggga gcgcgggagg
     1321 cggaggcaac gcgcaggtcg tgccgctgac ggagttctcc ctgggtctga ccggcgatat
     1381 agaccgcatc atgaacgccc acaacatcgc catggtcgct ctcacggccc ggatgcagca
     1441 cgagcgcaac tacggtgacg gcgagttggc aaagagaggc cttgcgcgtc tcgatgtcga
     1501 ccccacgaac atccagatgg gatgggtcat agacttctgc gctcaggcgc tccgcaacat
     1561 cgtcgtcggg atgggcggaa ggatggatgg cttcacgatg cagtcccact tcgatatcgg
     1621 ggtcagctcc gagctcatgg ccatcctgtc gatagtgcgg gacctcaagg acctgcgcaa
     1681 gcgcatcggc gagatcaccg tggcctatcg caagaacggc gagcccgtca ccaccgagga
     1741 cctcgaggtc gccggggcga tgtgcgccat catgaaggac accatcaatc ccactctgat
     1801 gcagaccgtg gaaggccagc ccatcatggt gcatgccggg cccttcgcga acatagccat
     1861 cgggcagtcg tcgatcattg tgggcgtgcc gcagaccttc ccgatcctgc gcccgccgcg
     1921 cggctgccag atcacctgct tcctgacgcc gagcacgaag agcgcctaca cgcatccccc
     1981 ggagctggca caggag
//
LOCUS       DAOQTR010000439         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_72824_length_1993_cov_3.269866, whole genome shotgun sequence.
ACCESSION   DAOQTR010000439 DAOQTR010000000
VERSION     DAOQTR010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72824_length_1993_cov_3.269866"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..603)
                     /gene="nikR"
                     /locus_tag="PKN85_09605"
     CDS             complement(199..603)
                     /gene="nikR"
                     /locus_tag="PKN85_09605"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNT44708.1"
                     /translation="MMKVRRFGVSLEEDLLEKLDRLSARHKFPNRSQAIRFLIRSALV
                     EGARSEDREVAGVIVIIYDHHKRGVVNRLLDIQHDYTDNIVSSQHIHLDHSNCLETIV
                     LKGRASRLQRLADRLLAIKGIKHGRLVVSGTE"
     gene            complement(734..1837)
                     /locus_tag="PKN85_09610"
     CDS             complement(734..1837)
                     /locus_tag="PKN85_09610"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNT44709.1"
                     /translation="MTTKIAVLTGGGDCPGLNGAVKWVVKTALDPLLADKRSMKFEVI
                     GVKEGWKGLVNVDPDDEESRNSYIEPLDEEKVRTWDRYGGTNLGTSRTNPFNPKNDRS
                     DLVIDNIGKLGIDVVVAIGGEDTLGVAYKLYKRGIKTIGIPKTIDGDLVGTDYSLGYD
                     SALNVVTEEIDRLRTTAGSHNRIFVVETMGRHAGWLALQGGESSGAYIILIPEYPFEL
                     ERVFELLRGRKGREVQYSIVVVSEGACLKGEGEFCKDERLDEFGHRTLGGIAAFLSDQ
                     IQRNTGFESRYVILSHLQRGGVPSARDRLMARWFGIAAVDMIVSEDFGRMVSYLHGEI
                     TSVPMKEIINKIKTVDLDKYYDIERYNGKRTIL"
BASE COUNT          397 a          588 c          547 g          461 t
ORIGIN      
        1 catccgcgag cacggcagcg acgccctgtt gttccttcag ctcacgcgcc tgaagaaaga
       61 catcgatctg ggcctgaacc tcaggtcttt ccgatatcgc aggacttagg ggcccctcgg
      121 gcggattgtc gctcgagaaa aaccccggac cctgagccga agaatcggag gccgcgcacg
      181 cccctgaggg cgggagtgct actcggtccc tgagacgacg agcctgccgt gcttgatacc
      241 cttgatggcc aaaagccggt ctgccagccg ctgcagccgg gatgcccggc ccttgagcac
      301 gatggtttcc agacaattgc tgtggtccag gtgtatatgc tggctggaga cgatgttatc
      361 ggtgtaatcg tgttgaatgt cgagaagccg gttgaccacg ccccttttgt gatgatcgta
      421 gataatgacg atgaccccgg cgacctcgcg gtcctcactg cgggctcctt ccaccagcgc
      481 gctcctgatg aggaacctga tggcttggct tctgttggga aacttgtgcc tggcggagag
      541 cctgtcgagc ttttccagga gatcttcttc aagagagacg ccgaaccttc ggaccttcat
      601 cataccacct cattggaaaa actatcacat gagtgcgcct gagaacaatc gtgttttcat
      661 cggcatgcat ccggatcggt acgccgttgt cctgtgatga tcatggggac actccggctg
      721 gatgcgaagt cgatcagaga atggtgcgct tgccgttata gcgttctata tcataatatt
      781 tgtccagatc cacggtcttg atcttgttga tgatctcctt catgggcacg gatgtgatct
      841 caccgtgaag atagctcacc atcctgccga aatcctcgct cacgatcatg tctaccgcag
      901 cgatcccaaa ccaccgcgcc atgagcctgt cccgggcgga ggggacgccc ccccgctgaa
      961 gatggctcag gatgacatat cgggattcga agccggtgtt gcgctggatc tgatcggaga
     1021 gaaacgccgc aatgccgccc aaggttctgt ggccgaactc gtccagccgc tcgtccttgc
     1081 agaactctcc ttctcccttg agacaggcgc cctcagatac gaccacgatg ctgtactgga
     1141 cctcgcggcc ctttctcccc cgcaggagct cgaacacccg ttccagctcg aagggatact
     1201 ccggaatgag aatgatgtac gcgccgctgc tctctccgcc ctgcaaggcg agccacccgg
     1261 catgtctgcc catggtctcc accacgaaaa tgcgattatg agatcccgca gtggtgcgga
     1321 ggcggtcgat ctcttcggtc acgacattca gcgccgagtc gtaccccagt gaatagtcgg
     1381 tccccaccag gtcgccgtcg atggtcttgg ggatgccgat ggtcttgatg ccgcgtttgt
     1441 agagcttgta ggccacgccg agcgtgtctt cccccccgat ggccaccacg acatcgatgc
     1501 caagcttgcc gatgttatcg atcacaagat cggaccggtc gttcttcggg ttgaacgggt
     1561 tggtacggga cgttcccaga ttcgtgcctc catagcggtc ccaggtcctg accttttctt
     1621 cgtccagggg ttcgatataa gaatttctgc tttcctcgtc gtcggggtcg acattcacca
     1681 ggcccttcca tccttccttc acacctatca cttcgaactt catggagcgt ttgtcggcca
     1741 ggagaggatc gagtgctgtc ttgacgaccc atttgacggc accgttcagg ccgggacagt
     1801 cgcctccgcc ggtaaggaca gcgattttcg tcgtcatggg cttcccccct tttctttcat
     1861 gcgcgatcac aaaggcatcg agtatgcctg aaataattat attctatttg atcgtctcgg
     1921 caagtcggtg ctaaatattg aaaccgactt gaaaaaccgg aggcagagat gaaagtggta
     1981 ttccatgagg act
//
LOCUS       DAOQTR010000440         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_73679_length_1978_cov_2.702028, whole genome shotgun sequence.
ACCESSION   DAOQTR010000440 DAOQTR010000000
VERSION     DAOQTR010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73679_length_1978_cov_2.702028"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKN85_09615"
     CDS             <1..634
                     /locus_tag="PKN85_09615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT44710.1"
                     /translation="TILLDEITEMDLKLQAKLLRVLQEKEVEALGSRSPRKIDVRVIA
                     TTNRDIVKTVGDGNFREDLYYRLSVFPITIPALRERRDDIAPLVNHLLKKHSRGMDIG
                     VSDEAMEHLTSRHWKGNVRELENVIARACILSNGSVITMSHLDEGRPAQDMAVGSVRD
                     METKLIIDALKSVRGNRSKAAGILGITVRTLRNKINEYRAMGIDVPAREY"
     gene            636..998
                     /gene="flgB"
                     /locus_tag="PKN85_09620"
     CDS             636..998
                     /gene="flgB"
                     /locus_tag="PKN85_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNT44711.1"
                     /translation="MQIVDLIGKALSVRAFYHKVISGNIANAQTPGYKEKEIDFRREL
                     DRRTGPEAGLAPSGDAGYTITENLGHDGLARLDGNTVSVEDQTVKLTENQLMYQALVQ
                     MASKRFAMMRYLIGEGKR"
     gene            1003..1434
                     /gene="flgC"
                     /locus_tag="PKN85_09625"
     CDS             1003..1434
                     /gene="flgC"
                     /locus_tag="PKN85_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNT44712.1"
                     /translation="MGAFDIFKISASGMKAQRKRMDIAASNLANVQTTNTEEGGPYRK
                     KDVVFKAADVSDDKSFSGTLSKKVEGVEIDSVKESDKPFQLVYNPSHPDANSEGYVTF
                     PNVNLMEEMTDMTAAARAYEANVNVLNTTKDMFLKTLDLIK"
     gene            1447..1734
                     /gene="fliE"
                     /locus_tag="PKN85_09630"
     CDS             1447..1734
                     /gene="fliE"
                     /locus_tag="PKN85_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNT44713.1"
                     /translation="MKVENFTLPSFGEVVRPANEKTRTSFGDVLKDSIKKVVELEKEA
                     DKEVEKLVKMENTDVQTTMIAIEKADLSFQMMMQIRNKIISAYEEIMRMQV"
     gene            1747..>1978
                     /locus_tag="PKN85_09635"
     CDS             1747..>1978
                     /locus_tag="PKN85_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44714.1"
                     /translation="MDELLNTTKNYLKTTPKSKLYMYLFLFIALVGGSIIGLSFLQKE
                     TYQTLFAGLSTEDASVVVAKLKELKVPYKLGLG"
BASE COUNT          516 a          546 c          578 g          338 t
ORIGIN      
        1 gaccatcctt ctcgatgaga tcaccgagat ggacctcaag ctccaggcaa agctcctgcg
       61 cgtgctccag gaaaaggagg tggaggccct cgggtcccgg tccccccgga agatcgacgt
      121 ccgagtcatc gcgacgacga acagggatat cgtgaagacg gtgggcgacg ggaacttccg
      181 ggaggacctg tactatcgcc tcagcgtctt tcccatcacc atcccggccc tgcgcgagcg
      241 ccgcgatgac atagcacccc ttgtgaacca cctgctgaag aagcattccc ggggaatgga
      301 catcggggtg agcgacgagg cgatggaaca cctgaccagc aggcactgga aagggaatgt
      361 ccgggagctg gagaacgtca tagccagggc gtgcatactt tccaacgggt ccgtcatcac
      421 catgagccac ctcgatgagg gcaggcccgc ccaggacatg gcggtgggct ccgtccggga
      481 catggagacg aagctcatca tcgacgccct caagtccgtc agggggaaca ggtcgaaggc
      541 cgcgggcata ctgggcataa ccgtccgcac gctgcgcaac aagataaacg agtacagggc
      601 aatgggcatc gacgtgcccg caagggagta ttgacatgca gatagtggat ctcatcggaa
      661 aggcgctctc cgtacgggcc ttctatcaca aggtgatctc cggcaacatc gcgaatgccc
      721 agacccccgg gtacaaggaa aaggagatcg atttccggcg ggagctcgac aggcgcaccg
      781 gccccgaggc gggactcgct ccctcaggcg acgcgggcta cacgataacg gagaacctgg
      841 ggcatgacgg cctcgcgcgc cttgacggaa acaccgtcag cgtggaagac cagacagtga
      901 agctcacgga aaaccagctg atgtaccaag cactggtcca gatggcctca aaacgttttg
      961 ccatgatgag gtatctcata ggcgaaggaa agaggtagga atatgggagc attcgatatc
     1021 ttcaagatca gcgcttcagg gatgaaggcc cagaggaaac gcatggacat cgcggcctcg
     1081 aaccttgcca acgtccagac cacgaacacg gaagagggcg ggccctacag gaagaaggat
     1141 gttgtattta aggcggcgga cgtctccgac gacaaaagct tctcggggac cctctcaaag
     1201 aaggtggagg gcgtcgagat cgactctgtc aaggagagcg acaagccatt tcagttggtc
     1261 tacaacccga gccatcccga cgccaattct gaaggatacg tcacatttcc caacgtgaac
     1321 ctgatggaag agatgacgga catgacggcg gccgcccgtg cctacgaggc gaacgtcaac
     1381 gttctcaata cgacgaagga catgttcctg aagactctcg accttattaa ataggggggc
     1441 ggacccatga aggtagaaaa ctttactctt cccagttttg gcgaggtcgt aagaccggcc
     1501 aacgaaaaga ccaggacctc cttcggtgat gtcctgaagg actccatcaa gaaggtcgtg
     1561 gaactcgaga aggaagcgga caaggaagtg gaaaaactgg tcaagatgga gaacaccgat
     1621 gttcagacca cgatgatcgc gatcgagaaa gcggacctgt ccttccagat gatgatgcag
     1681 atacggaaca agatcatctc cgcttatgaa gagataatga gaatgcaggt gtaacgtatg
     1741 cctccgatgg acgaacttct caacaccacc aagaactatc tcaaaacgac cccgaagagc
     1801 aaactctaca tgtacctctt tcttttcata gcgctcgtgg gaggctccat catcggtctt
     1861 tccttcctac agaaagagac ctaccagacg ctattcgccg gcctctccac ggaggacgcc
     1921 tcggtggtcg tggcgaagct caaggagctc aaggttccct acaagctggg tcttggcg
//
LOCUS       DAOQTR010000441         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_73935_length_1974_cov_3.476811, whole genome shotgun sequence.
ACCESSION   DAOQTR010000441 DAOQTR010000000
VERSION     DAOQTR010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73935_length_1974_cov_3.476811"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKN85_09640"
     CDS             <1..297
                     /locus_tag="PKN85_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44715.1"
                     /translation="ESIAVRLDEKKSVTFSGDTDISANLVRLADGTDTLVAECAFPER
                     KVKGHLNLAALDRIVERAQPKRVILTHLYPDWDDWAGVLHAPYLLGEDGMEMEV"
     gene            521..1255
                     /locus_tag="PKN85_09645"
     CDS             521..1255
                     /locus_tag="PKN85_09645"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNT44716.1"
                     /translation="MRELVAGIVRPARRFPGGRGAPGVFELGGRTVVCRQYLHGGWLR
                     GVTGGNFLGEDRALRELEVTVYLEEKGFPVVSPVGYVAENQASSRKLYFLSLFLPDAH
                     DLMTHFASGGTGERLRMAKRLAEALFEMGRLGVYHPDLHLRNVLVTSSGGLFLLDFDK
                     AYRKEVTSADYERMFWRLDRFVRKYASLFGHPVDDRERLIFLRTYERLSGDRIVERMA
                     QKRDKMHRTSKLGWTIDRLIYRRKKT"
     gene            complement(1338..>1974)
                     /locus_tag="PKN85_09650"
     CDS             complement(1338..>1974)
                     /locus_tag="PKN85_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT44717.1"
                     /translation="VPFLPPREVPERFSLEPKDIVERTLGAGRGVIALTFHFGNWEIM
                     GITSKLLNQPVVALARPLKKHTRLNAFLNRLRESTGLTIIVNANTAKEVMRLIRKNSI
                     IAILGDQREKRSRGVFVDLFGHKVPTSKGTAMIAMKTGCQVVPCYPVRKGFLRYTIMC
                     GEPLVMERDGDIEDLIAKNTRKINAFLEDIIRKYPDEWFWVHQRWGRKRRA"
BASE COUNT          403 a          538 c          632 g          401 t
ORIGIN      
        1 gaaagcattg ccgtgaggct cgacgagaag aagtctgtca cgttctcggg cgacaccgat
       61 atctctgcca acctcgttcg actggccgac ggaacggata ccctcgtggc ggagtgtgcc
      121 tttcccgagc gaaaggtgaa ggggcacctc aaccttgccg cccttgacag gatcgttgaa
      181 cgagcgcagc cgaagcgtgt catccttact cacctttacc ccgactggga cgactgggcc
      241 ggcgttctcc acgcaccgta ccttctgggg gaggacggga tggagatgga agtgtaaaga
      301 ccgtcgcggg tttcaggtct caggtctcag gttaaagaca aagaaacaga atgttcctga
      361 tggctggcat gtgcctcgcc aggtatgcag acctccaggg gcttttctct tgtttccgtg
      421 gaaaaatgcg tttgtatcgt atacaatcag gaaaatggca aaactcaaaa gggaacggga
      481 agggaggtat acctttgttc accgagggga gtcggtggat atgagggagc ttgttgccgg
      541 gattgtaagg cccgcaagga ggttccccgg ggggcggggc gcgccgggtg tcttcgaact
      601 cgggggcagg acggtcgtct gcaggcaata cctccacggc ggatggctcc gtggggtaac
      661 gggcggcaac ttccttggcg aggacagggc gctcagggaa cttgaggtca cggtctacct
      721 ggaagagaag ggatttcccg tggtcagccc cgtcggctat gtggcggaga accaggcatc
      781 ctcccggaag ctctacttcc tctccctttt tctgcccgat gcccatgacc tcatgacgca
      841 ctttgcctcc gggggtaccg gggagcgcct gcggatggcg aagaggctcg ccgaagcact
      901 ctttgaaatg ggtcggctgg gagtctatca tcccgacctg catctcagga acgttctcgt
      961 gacctcaagc ggagggcttt tcctcctcga cttcgacaag gcataccgca aggaagtgac
     1021 atcggccgac tacgaaagga tgttctggcg tctcgacagg ttcgtgagga aatacgcctc
     1081 tctcttcggc caccccgtag acgacaggga acgcctcatt ttcctgcgca catacgaacg
     1141 cctctccggc gacaggatcg tggagaggat ggcccaaaaa cgcgacaaaa tgcaccgcac
     1201 ctcaaaacta ggctggacca tagaccgttt gatctataga agaaagaaga cctgaaaagc
     1261 cggcgccaga tcctggcgac acgcggcaac gtccgtcggc ggtaccccgt tcaagccatt
     1321 caagcggctc aagcgattca agcccgcctc ttcctccccc accgctggtg gacccagaac
     1381 cattcgtcgg ggtacttgcg gatgatgtct tcgaggaagg cgttgatctt gcgggtgttc
     1441 ttcgcgatga ggtcttcgat gtcgccgtca cgttccatga cgaggggttc gccgcacatg
     1501 atggtgtagc gcaggaagcc cttgcgtacg gggtagcagg ggacgacctg gcagccggtc
     1561 ttcatggcga tcatggcggt gcccttcgat gtgggaacct tgtgaccgaa gagatcgacg
     1621 aatacgccac gggagcgttt ctcgcgctgg tcgccgagga tggcgatgat ggaattcttc
     1681 cggatcaggc gcatgacctc cttcgccgtg ttggcgttga cgatgatggt gaggcccgtc
     1741 gattccctga ggcgattgag aaaggcgttg agccgggtgt gtttcttcag gggcctggcg
     1801 agggccacga cgggctggtt gaggagcttg gaggttatgc ccatgatctc ccagttgccg
     1861 aagtgaaagg tgagcgcgat gacgccgcgg cctgccccaa gggttctttc cacgatgtcc
     1921 ttcggttcca gagagaacct ctccgggacc tcgcggggag ggagaaaggg gacg
//
LOCUS       DAOQTR010000442         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_73983_length_1973_cov_4.517205, whole genome shotgun sequence.
ACCESSION   DAOQTR010000442 DAOQTR010000000
VERSION     DAOQTR010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73983_length_1973_cov_4.517205"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKN85_09655"
     CDS             <1..560
                     /locus_tag="PKN85_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNT44718.1"
                     /translation="EGDTAAALKGQDVLIALSKSGPDVIQKEWMASMADNAIVFVCAN
                     PIPEMWPWDAKEAGARIVATGRSDFPNQVNNSVGFPAIFRGTLDVRARTITDEMCIAA
                     AYELARCAEDRGLTEDHLLPTMNEWEVFPREAVAVAKMAMKQGVARLTIDEKELYNMA
                     VTKIRRAQDEVGLLMEKGIIAPYAG"
     gene            570..1409
                     /locus_tag="PKN85_09660"
     CDS             570..1409
                     /locus_tag="PKN85_09660"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNT44719.1"
                     /translation="MREIHVDDIVSVVERLFIDANISLPEAMYGAIRDAIEKESSPVG
                     KEVLRELLRNADTAREEHMPICQDTGLAVTFLEIGQDAHVVGGDLTEAVTEGVRRAYR
                     NGYLRKSCCHPFSRKNTGDNTPPIIHTKIVPGDGMKITVLPKGGGSENYGEVRMLMPA
                     EGKEGVKAFVLEMVRKGGPNPCPPITVGVGIGGNFETSALLSKEALMVPVGTRNDDEE
                     LAAMERELLEEINATGIGPQGYGGTVTALDVHIKMAPCHLASLPVAVNIQCHAHRIGE
                     ALL"
     gene            1406..1963
                     /locus_tag="PKN85_09665"
     CDS             1406..1963
                     /locus_tag="PKN85_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HNT44720.1"
                     /translation="MTTDLRRITTPLTDDVTGSLTAGEKVLLSGFIHTARDAAHKRFI
                     ETLDAGGDLPFDIRGQIIYYCGPAPAPPGKVIGSCGPTTSSRMDVYAPRLIAMGLRGM
                     IGKGKRSEGVKGALREFRAVYFGATGGAGALLARAVVAADVIAYEDLGPEAVVRLEVR
                     DMPLFVINDVHGNDLYLEGIERYRK"
BASE COUNT          424 a          564 c          647 g          338 t
ORIGIN      
        1 tggaaggcga caccgcggcg gcgctcaaag gacaggacgt tctcattgcc ctgtcgaagt
       61 cgggaccgga cgttatacag aaggaatgga tggcgtcgat ggcagacaac gcgatagttt
      121 tcgtctgcgc caacccaatc cccgagatgt ggccctggga cgcgaaagag gcgggggccc
      181 gcatagttgc cacgggcagg agcgatttcc caaatcaggt gaacaattcc gtcgggtttc
      241 cggcgatatt ccggggcacc ctcgacgtca gggcccggac catcaccgac gagatgtgca
      301 tagccgcggc ctacgaactg gcccggtgcg ccgaggacag ggggctcacc gaagatcacc
      361 tccttccgac gatgaatgag tgggaggtct tccccaggga agctgtggcc gtcgcgaaga
      421 tggcaatgaa acagggcgtc gctcgcctta ccatcgacga aaaggaactc tacaacatgg
      481 cggtcacgaa gatccgcagg gcccaggacg aggtgggcct gctcatggag aagggcatca
      541 tagcgcccta cgccggataa ggagccccca tgcgcgagat ccacgtggac gatatcgttt
      601 ccgttgtcga acgtttgttc atcgacgcca acatcagtct tcccgaggcc atgtacggtg
      661 ccatccggga cgcgatcgag aaggaaagct ctcccgtcgg caaggaggtg ttgagggaac
      721 tcttgaggaa cgccgacacg gcccgggagg aacacatgcc catatgccag gacacaggtt
      781 tggcggtgac ctttctcgag atcggccagg acgcgcacgt tgtgggagga gacctcaccg
      841 aggccgtgac ggagggcgtc cgcagggcgt accgcaacgg atatctcaga aaatcctgct
      901 gccatccttt cagcaggaag aataccgggg ataatacgcc tcccatcatc cacacgaaga
      961 tcgtgcccgg ggatgggatg aagatcactg ttctgccgaa gggcggagga agcgagaact
     1021 acggggaggt ccgcatgctc atgcccgctg agggcaagga aggggtcaag gcctttgtcc
     1081 tcgagatggt gcgcaaggga ggccccaacc cctgtccgcc gatcaccgtc ggtgtgggca
     1141 tcgggggcaa tttcgagacc tcggctctcc tgtcgaagga ggccctcatg gttcccgtgg
     1201 ggacgcggaa cgatgacgag gaactggcag ccatggagag ggaattgctc gaggagatca
     1261 acgcgaccgg cataggacct caaggatacg ggggcacggt gacggccctc gacgttcaca
     1321 taaagatggc gccctgccat ctcgcgtcgc tacccgtggc ggtcaacatc cagtgccacg
     1381 cgcaccgcat cggggaggcc cttctatgac gacggacctc aggcggataa cgacacctct
     1441 taccgatgac gtgacggggt ccctcacggc gggagagaag gttcttctct ccggtttcat
     1501 ccataccgcc cgggatgccg cgcacaagag attcattgaa accctcgatg ccggcgggga
     1561 cctgcctttt gacatcaggg gacagatcat ctactattgc ggtccggcgc cggcaccgcc
     1621 gggaaaggtc atcgggtcct gcggacccac gacgagttcc cgcatggacg tctacgcgcc
     1681 gaggctcatc gccatgggcc tcaggggcat gatagggaag ggtaaacgtt ccgaaggggt
     1741 caagggagcg ctcagagagt tcagagcggt ctattttggg gcgacaggag gggccggcgc
     1801 gctgctcgcg cgcgccgttg tcgccgcgga cgtgatcgcc tatgaagacc ttggtcctga
     1861 ggcggtagtc aggctcgagg tgagggacat gccgcttttt gtcatcaacg acgttcatgg
     1921 caacgacctg tacctggaag gcattgaacg ctacaggaag tgatgtccct gct
//
LOCUS       DAOQTR010000443         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_74460_length_1965_cov_3.301571, whole genome shotgun sequence.
ACCESSION   DAOQTR010000443 DAOQTR010000000
VERSION     DAOQTR010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74460_length_1965_cov_3.301571"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..934
                     /locus_tag="PKN85_09670"
     CDS             248..934
                     /locus_tag="PKN85_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT44721.1"
                     /translation="MTSQLIAVVDDEPDILELVSLHLKRSSFRVSGFADAQSFYQFLA
                     KEVPDLVILDLMLPDVDGLEICKYLRGQDRFSSIPVIMLTAKTEETDKVIGLELGADD
                     YVTKPFSPRELVARVRAVLRRHGRTDDDARRMVISNLVAIDLDRHEVEVEGNRVELTP
                     TEFRILELLASRRGRIQSRDQILTHLWGDEKYVIDRTVDVHIRHLREKLGKAARLIKN
                     VRGAGYRVEE"
     gene            931..>1965
                     /locus_tag="PKN85_09675"
     CDS             931..>1965
                     /locus_tag="PKN85_09675"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNT44722.1"
                     /translation="MKRSLFFKIFGGYLVLTVALSGLIVLISSYLIRNYQIERTAREL
                     KEIAIVLGERTLGMPSEGAAAIDRFVKEMGKKINSRITLVAPDGRVIADSEEDPARME
                     NHRTRIEISQALEGVTGRFLRLSDTLGQEMLYIAVPVEREGKTLYVLRTSKFLKDIKT
                     VSRELNAKILLVTVIILVAALIGAFIFARTIYRPIGELSAAIKRVAGHDFNTRVLLKA
                     QDELKEVADSFNDMTDEMQGLFADLTRQKEELNSIISSLQEGLLVLDGEERVLLLNDS
                     LKTLMGRDVEIGKPYWEIIREPALNELVNRVHMEKLNRVGEMTINGAVFLCSATYLGP
                     REEIVLVFHDI"
BASE COUNT          446 a          552 c          594 g          373 t
ORIGIN      
        1 gaacggtgat gggcgggatc cgcgcgattt gggcatgaag aaatgcttcg atgaaaaact
       61 cctcagaaag ttcatagtgt tcctgggaaa agtctcgtca cagatgtgat atctccggac
      121 cgagaaaggc gtaccccttt ctggtcgcga aaaaacccgc ggtcttaatc caaaattaac
      181 aatcggccct tagtatggaa gggcgaaacg gctttgcaaa gagcaatgga tgaggtaatc
      241 tcatagtatg accagtcaat tgatagcggt cgtcgacgac gaaccggaca tactggagct
      301 ggtgtcgctc cacctgaaga gatcgagctt ccgtgtgagc ggctttgcgg acgctcagtc
      361 cttctaccag ttcctcgcga aggaggtgcc ggacctcgtc atactcgacc tcatgcttcc
      421 cgacgtggat gggctggaga tctgcaaata tctgaggggg caggaccgtt tttcctccat
      481 ccccgtgatc atgctcacgg cgaagacgga ggagaccgac aaggtcatcg gcctggaact
      541 gggggctgac gactacgtga cgaaaccctt ttccccccgt gagctcgtgg cgcgggtgcg
      601 ggcggtcctg aggcgtcacg gccggaccga cgacgatgcc cgcaggatgg tcatttccaa
      661 cctggtcgcc atcgatctcg acaggcacga ggtggaggtg gagggaaaca gggtggagct
      721 cacgccgacg gaattcagga tactagagct cctcgcctcc cggcggggac gcatccagtc
      781 gcgggaccag atactgaccc atctctgggg agacgaaaag tacgtcatag accgaaccgt
      841 ggacgtccac atcaggcacc tgcgcgagaa gctcgggaag gccgcccgcc ttataaagaa
      901 cgtccgtggc gcgggataca gggtggagga atgaagcgtt ccctcttttt caagatattc
      961 ggcgggtatc ttgtcctcac cgtcgccctt tccggcctca tcgttctcat ctcgtcctat
     1021 ctcatccgca actaccagat agagaggacc gcgcgggaat tgaaggagat cgccatcgtc
     1081 ctcggggagc gaacgctggg gatgccgtcg gagggagccg ccgccattga ccgcttcgtg
     1141 aaggagatgg gaaagaagat caacagccgc atcacccttg ttgcccctga cggcagggtc
     1201 atcgccgact ccgaggaaga ccctgccagg atggagaacc accgcacgag gatcgagatc
     1261 tcccaggcgc ttgaaggcgt cacggggcgg ttcctgcgct taagcgacac cctcggccag
     1321 gagatgctct acatcgccgt tcccgtcgag agggaaggga agaccctgta cgtccttcgt
     1381 accagcaagt tcctcaagga tataaagacc gtttcccggg aactgaatgc gaagatattg
     1441 ctcgtgaccg tcatcatcct cgtcgcggcg ctcatcgggg cgttcatctt cgcgcggacc
     1501 atctaccggc cgatcggcga gctgagcgcc gccataaaga gggtggccgg ccacgatttc
     1561 aacacccggg tgttgcttaa ggcgcaggac gaactcaagg aggtcgccga cagtttcaac
     1621 gatatgaccg atgagatgca ggggcttttc gccgacctta cccgtcagaa ggaagaactg
     1681 aacagcatca tctcctccct gcaagaggga ctcctcgtgc tcgacggcga ggagagggta
     1741 cttcttctca acgacagcct gaagaccctg atgggccgcg atgtggagat tgggaaaccc
     1801 tactgggaga tcataaggga gccggcgctc aacgaactgg tgaacagggt ccatatggag
     1861 aagctgaacc gcgtcgggga gatgacgata aacggagcgg tcttcctgtg cagcgccacc
     1921 taccttggcc cccgggaaga gatcgtcctc gtcttccatg acatc
//
LOCUS       DAOQTR010000444         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_75023_length_1956_cov_3.266702, whole genome shotgun sequence.
ACCESSION   DAOQTR010000444 DAOQTR010000000
VERSION     DAOQTR010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75023_length_1956_cov_3.266702"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1221
                     /locus_tag="PKN85_09680"
     CDS             76..1221
                     /locus_tag="PKN85_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT44723.1"
                     /translation="MELTTEQKDICKAAREFAEQEFRDKAKEFDEKEEFDLSIVKRAA
                     ENGFIGVFIKEDYGGAGLGFLEHSLITEEVWRVDPGCGQNICSAGFGAEMIQTFGTEE
                     QKQFYLPKIASGEAIIGTAITEPDAGSDVTMVKTRAEKKGDRYVINGAKMFITNGCNA
                     KYLLVYAVTDPEAKDVHKRCSVILLDTATPGFAATKIYGKMGIRASNTAEVSFDNVEV
                     PAENLIGKEGRGFYQLMEFFNMTRNHVAAQGVGVAQGALEMAISHVKKRKAFGGSLSR
                     LQGVQFKLADMATRIEAARSLYWRAAYLLDNGKLDPALVSMAKWFSGETAVYVANEAL
                     QLHGGYGYIIEYDIQRFYRDAKIVEIYEGSKEVEKAVIASELLKRVF"
     gene            1617..>1956
                     /locus_tag="PKN85_09685"
     CDS             1617..>1956
                     /locus_tag="PKN85_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT44724.1"
                     /translation="MVEQAATRVLTCIQCGKCTGSCPESGKTPFNVRMLVRKKQFQSA
                     IDEALPWYCTSCGACTIRCPRDVKPSEVIIELRAKLVEDGDIPVALQKALENTFVQKN
                     PWGRSRNKRAD"
BASE COUNT          438 a          581 c          581 g          356 t
ORIGIN      
        1 ccgcaagcag ttcggcgcgg cctgacggat ggttggcggc acacaggctc ggaataacct
       61 tttggaggaa gacagatgga actgacaacg gaacagaagg acatatgcaa agcggcccgt
      121 gaatttgccg agcaggagtt cagggacaag gcgaaggagt tcgacgagaa ggaggagttc
      181 gacctgtcca tcgtgaagcg cgccgccgag aacggcttca tcggggtctt catcaaggag
      241 gactacggtg gagcgggcct ggggttcctc gagcattcgc tgatcaccga agaggtgtgg
      301 cgtgtcgacc ccggctgcgg ccagaatatc tgttccgccg gtttcggcgc cgagatgatc
      361 cagaccttcg gaacggagga gcagaaacag ttctatctcc cgaagatcgc ctccggggag
      421 gccatcatcg gcaccgccat aacggagccc gacgcgggca gtgacgtgac gatggtgaag
      481 acccgcgccg agaagaaggg agacaggtac gtcatcaacg gcgcgaagat gttcataacg
      541 aacggctgca acgcgaaata ccttctcgtt tacgccgtca ccgatcccga ggcgaaggac
      601 gtgcacaagc ggtgcagcgt catactcctc gatacggcga cccctggttt tgcggcgacg
      661 aagatctacg gcaagatggg catcagggcc tcgaacactg cggaggtgag cttcgacaac
      721 gtggaggtcc ccgccgagaa cctcatcggc aaggaaggcc ggggcttcta ccaactcatg
      781 gagttcttca acatgacccg caaccacgtc gccgcccagg gtgttggcgt cgcccagggc
      841 gccctcgaga tggccatctc ccacgtgaag aagagaaagg ccttcggcgg atccctctcg
      901 cggctccagg gcgtgcagtt caagctggcc gatatggcca cccgcatcga ggccgcccgc
      961 agtctctact ggcgcgccgc gtacctcctg gacaacggca agctcgaccc ggcgctcgtc
     1021 tcaatggcca agtggttctc cggcgagacc gccgtctacg tggccaacga ggccctccag
     1081 ctccacggcg gctacggcta cataatcgag tacgatattc agcggttcta ccgcgacgcg
     1141 aagatagtcg agatctacga aggctccaag gaggtcgaga aggcggtcat cgccagcgag
     1201 ctcctgaaac gcgtcttcta gacgcggctt gaaccgcttg aatggcttga gcctgttcaa
     1261 cgtttaaaga caacaatggc ccctgtcgtg cgcggtcacg caagggtcat tgtttttttt
     1321 ccaacgccct gcagcctgtt tgcaaagtgg gtttccactc cgattcgtca cccccttctc
     1381 tcaaacgatc tcaacgatct gatcctcccc aggcctgtat caaaaggtta cacctgttcc
     1441 caaaccggac acgcgggggg tgttgcatcc tgtgccacgt ttgggctaac ataccataat
     1501 aattggcgac gggctgcccg gcttgctttg gtatccccat tgcatacatc cttgcacgga
     1561 gacatggaga acaactaccg ccatacaaat ggaatcacca tcgggggtta cgtataatgg
     1621 ttgaacaagc cgcaaccagg gtcttgacct gcatccagtg cgggaagtgc acggggagtt
     1681 gccccgaatc gggcaagacg cctttcaacg tgaggatgct cgtcaggaag aaacagttcc
     1741 agtctgccat cgacgaggcc cttccatggt actgcacgtc ctgcggcgcc tgtaccatac
     1801 ggtgcccgag ggacgtgaag ccctcggagg tcatcatcga gcttcgcgcg aaactcgtcg
     1861 aagacggtga catacccgtc gcgctccaga aggccctgga gaataccttc gtgcagaaaa
     1921 acccctgggg gcgctcccgc aacaagcgtg ccgact
//
LOCUS       DAOQTR010000445         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_75131_length_1954_cov_3.959452, whole genome shotgun sequence.
ACCESSION   DAOQTR010000445 DAOQTR010000000
VERSION     DAOQTR010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75131_length_1954_cov_3.959452"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKN85_09690"
     CDS             <1..838
                     /locus_tag="PKN85_09690"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNT44725.1"
                     /translation="VVPYVRGREESRKSGDVLAEVREMAERGIVDVTLLGQNVNSYNN
                     GNKDLSFPELLSGIDAIPGISRLRFVTSHPKDISPELIACFGTLRTLCESIHLPFQSG
                     SDRILALMNRGYTSSEYLGKVEALKDRCPGIALTADCIVGFPGEEDGDFEATMRLMET
                     VRFDGVFSFIYSARKSTAAAALTGRVPPAVAHERLSRFQKRQRAITLDSNRAMEGTRV
                     EVLVEGQSKNSPDELSGRTRTGKIVNFRGDPGLVYKLVEVDIIKGYANSLRGELPDTP
                     GR"
     gene            842..1342
                     /locus_tag="PKN85_09695"
     CDS             842..1342
                     /locus_tag="PKN85_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNT44726.1"
                     /translation="MLKEMKVAGITVDPFTNTPIVLLKDSEEKDVLPIWIGLLEASSI
                     ATALENIETPRPMTHDLIKNLLDSLGVKILKIEVNDLKDNTYYALIHLDVNRKRMVID
                     SRPSDAIAIALRTNAPIFVEESVIQRSAKVDLATQKSDKVVTETSDWEDILENLSQDD
                     FGKYKM"
     gene            1400..>1954
                     /locus_tag="PKN85_09700"
     CDS             1400..>1954
                     /locus_tag="PKN85_09700"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HNT44727.1"
                     /translation="MIDLHTHTLLSDGELLPLELARRAKVKGYRILGISDHVDVTNIE
                     NVAVSILKLLKYAELHEGIRIVAGAELTHCPARQIGELTAMARRLGFSYVVVHGETIA
                     EPVEEGTNAAAIEAGVDILAHPGLITQREVEAARDRGVLLEITTRKGHSLTNGHVAKL
                     AGAAGAKLVINTDSHGPSDLVTDGE"
BASE COUNT          464 a          581 c          570 g          339 t
ORIGIN      
        1 cgtggtgcct tacgtgcggg gtcgggaaga aagcaggaag agcggcgatg tccttgccga
       61 ggtccgcgaa atggccgaaa ggggcatcgt ggacgtgact ctcctcggcc agaacgtcaa
      121 ctcatataat aatggtaata aggatctctc cttcccggaa ctcctcagcg ggatagatgc
      181 gataccgggg atatcccggc tgcgttttgt cacgtcccat cccaaagaca tctcgcctga
      241 gctcatcgcc tgtttcggga ctttgcggac actctgcgaa tcgatccacc tccccttcca
      301 gtcgggttcc gacaggatcc tcgccctcat gaacaggggc tacacctctt ccgagtacct
      361 cggcaaggtc gaagccctca aggataggtg ccccggcatc gccctcaccg ccgactgcat
      421 cgtgggcttc ccgggcgagg aggacggcga tttcgaggcg accatgaggc tcatggagac
      481 cgtgaggttc gacggggtct tttccttcat ctactcggca cggaaatcca ccgccgcggc
      541 cgccctgacc ggcagggtgc ctcccgccgt cgcccacgaa aggctcagcc gattccaaaa
      601 gaggcagagg gcgataaccc tcgactccaa cagggcgatg gagggcaccc gggtcgaggt
      661 ccttgtcgaa ggccagagca aaaactcccc cgacgaactc tcaggaagga cgcggaccgg
      721 caagatagtc aacttccggg gcgaccccgg gctggtttac aaattagtgg aagtggatat
      781 aataaaaggg tatgctaatt cattacgggg agaactaccg gataccccgg ggaggtaaac
      841 gatgctgaag gagatgaagg ttgccggaat aaccgtggac ccttttacca atacacccat
      901 cgttctcctg aaggattcgg aggaaaagga cgtcctcccc atctggatag ggcttctcga
      961 ggcatcgagc atcgcaacgg ccctggagaa cattgagacg ccgagaccca tgacgcacga
     1021 tctcatcaag aacctgctcg acagcctggg ggtgaagatc ctcaagatcg aggtgaacga
     1081 tctcaaggac aacacctact atgcccttat ccacctcgac gtgaacagaa agcggatggt
     1141 catcgattcg cgtcccagcg acgccatcgc catcgccctt cgcacgaacg cccccatctt
     1201 cgtcgaggag agcgttatcc agcgctcagc caaggtggac ctggccacac agaaaagtga
     1261 caaggtggtc acggaaacct ccgattggga agacatactg gaaaacctct ctcaggacga
     1321 ttttggaaag tacaagatgt agcaataccg aagatagaca caaccagggc ggaaaagagc
     1381 cctttatgat ccggaaatta tgatcgattt gcatacacat accctcctga gcgacgggga
     1441 actgcttccc cttgagcttg cccgccgggc aaaggtcaag ggttaccgga tactcggcat
     1501 atcggaccac gtggacgtga cgaacataga gaacgtcgcc gtgtccatcc tgaaacttct
     1561 gaaatacgcc gagctgcacg agggcatcag gatcgtggcg ggggcggagc tcacccactg
     1621 ccccgcgaga cagatcggtg agctcaccgc catggcacgc aggctcggct tctcctacgt
     1681 cgtcgtgcac ggcgagacca tcgcggagcc cgtggaggaa gggacgaatg ccgcagccat
     1741 agaggcgggc gtcgatatcc tcgcccatcc cggcctcatc acccaaagag aggtggaggc
     1801 ggccagggac cgcggggtcc tcctggagat aaccacgcgg aaggggcatt ccctgacgaa
     1861 cggtcacgtc gcgaagctcg ccggggccgc gggggcgaaa ctcgtcatca acaccgattc
     1921 ccacggtccg tcggacctcg tcacagatgg agag
//
LOCUS       DAOQTR010000446         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_75145_length_1954_cov_3.328067, whole genome shotgun sequence.
ACCESSION   DAOQTR010000446 DAOQTR010000000
VERSION     DAOQTR010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75145_length_1954_cov_3.328067"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKN85_09705"
     CDS             complement(<1..514)
                     /locus_tag="PKN85_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44728.1"
                     /translation="MEKAGTDLTKRSIWRTLDIPIIIAGFLVILPILGINRTTDFAIF
                     CIFVLAFNILYGFMGRLSFGHMLYLGTGAYAVTLFSEHVSGNPLLAILAALVAGALIG
                     VILGPIIVRTTGACFALINLAFCQVGYFLVLVAFSRYTGGEDGMSAYFSKMPLLNFGN
                     KGIVFGFVLFC"
     gene            complement(605..1279)
                     /locus_tag="PKN85_09710"
     CDS             complement(605..1279)
                     /locus_tag="PKN85_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HNT44729.1"
                     /translation="MAVQNKMPKIDAKKTAIVLIEPQYDFLKPGGSMYQFIADQLEKR
                     HVIENLADLVAKARKKVKKIIYIPFEAFEPGFPEIDKEGPGMGGLRGLEIDMPTGWTL
                     HGKKVAGAWVAGTPGPEIIPELTPQKGDIIVRGKKTLDAFWSTALDYTLRVNMIEHVA
                     IVGFHTNWCVESTARSAYDHGYRVIVIGDCTGTDTDEEQNYAEKYIFPKIGHVMGYKE
                     FLKSLK"
     gene            complement(1343..>1954)
                     /locus_tag="PKN85_09715"
     CDS             complement(1343..>1954)
                     /locus_tag="PKN85_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT44730.1"
                     /translation="GSMVFMAGRNGAGKTTFLKSIAGFMKPIAGSISFLGRDISGMAP
                     EKVALMGIRYVFQDKRVFSNLTVRENLELAAYPVKEKTSHAIEKVMSIYPKMDKFLNL
                     RAGSLSGGQRQILLIGRALVGDPKLLLVDEPTEGLAAGTINDILNVLAVMKGKVSMII
                     VEQNLSVVGMLAEKVYIMREGKIVQTCTSKEEIADKERLEQYL"
BASE COUNT          359 a          578 c          581 g          436 t
ORIGIN      
        1 ggcagaaaag gacgaacccg aacacgatgc ccttgttccc gaaattaagg agcggcatct
       61 tcgagaaata ggccgacatg ccgtcctccc cgcccgtgta ccgggaaaag gcgacgagga
      121 cgaggaagta gcccacctgg caaaaggcaa ggttgatgag cgcgaagcac gctcccgtgg
      181 tgcgcacgat gatgggaccc aggatgaccc ctatgagggc ccccgcgacg agcgccgcca
      241 ggatggcgag cagcgggttc ccggagacgt gctccgagaa gagggtaacc gcgtaggctc
      301 cggtgccgag gtagagcatg tgcccgaagg aaagtctgcc catgaagccg tagaggatgt
      361 tgaaggccag gacgaagatg cagaagatgg cgaagtccgt cgtcctgttt atcccaagta
      421 tggggaggat gacgaggaac ccggcgatga tgatggggat gtccagcgtc cgccagatgc
      481 tcctttttgt gagatccgtt ccagcttttt ccatggcctt tttaagcctc cgtctcgcat
      541 ctcctgaccc gctcccccgt cgggagagcg ggtcaggagg gtcatatggt gcttaagtct
      601 cgcgtcactt gagactcttg agaaattcct tgtatcccat cacgtgcccg atcttgggga
      661 agatgtactt ctccgcgtag ttctgttcct cgtccgtgtc cgtgccggtg cagtcgccga
      721 tgacgatgac gcggtagccg tggtcgtagg cggaacgggc cgtggattcc acgcaccagt
      781 tcgtgtggaa gccgacgatc gccacgtgct cgatcatgtt gaccctgagc gtgtagtcaa
      841 gggccgtgga ccagaaggca tcgagggtct tcttgccgcg gacgatgatg tcgcctttct
      901 gcggcgtcag ttcggggatg atctccgggc ccggcgtacc ggccacccag gcaccggcca
      961 ccttcttccc atgaagcgtc catcccgtgg gcatgtcgat ctcgagcccc cgcaggccgc
     1021 ccatgccggg gccctctttg tctatctccg ggaaaccggg ttcaaaggcc tcgaagggga
     1081 tgtagatgat cttcttcact ttcttgcgtg ccttcgcgac gagatcagcc aggttctcta
     1141 tgacgtggcg tttttcgagc tggtccgcga taaactgata catggaaccg ccgggtttca
     1201 ggaaatcgta ctgcggctcg atgaggacga tggccgtctt cttcgcatcg atctttggca
     1261 ttttgttctg tactgccatg agctgcctcc tctttatggt cttttttatc tatatctgcg
     1321 gcgtgccgcc cacttgcctg actcacaggt actgctccag ccgctccttg tccgctatct
     1381 cctctttgga cgtgcatgtc tgcacgatct tgccttcgcg catgatgtat accttctcgg
     1441 cgagcatccc aacgacggag aggttctgct cgacgatgat catggagacc ttgcccttca
     1501 tcacggcaag gacgttcagt atgtcgttga tggtccctgc cgcgaggccc tccgtgggct
     1561 cgtcgacaag cagcagcttc ggatcgccga cgagcgccct gccgataagc aggatctgcc
     1621 gctggccgcc gctcaagctc ccggccctca ggttcaggaa cttgtccatc ttcgggtaga
     1681 tgctcatgac cttctctatc gcgtgggacg tcttctcctt cacggggtag gcggcaagtt
     1741 ccaggttctc ccggacggtg aggttcgaga agaccctttt gtcctggaag acgtaccgta
     1801 tacccatgag cgccaccttt tccggcgcca tgcccgagat gtcccttccc agaaaagata
     1861 tgctgcccgc gatgggcttc atgaagccgg cgatggactt gaggaaggtc gtcttcccgg
     1921 cgccgttgcg gcccgccatg aagaccatgc tgcc
//
LOCUS       DAOQTR010000447         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_75147_length_1954_cov_3.238547, whole genome shotgun sequence.
ACCESSION   DAOQTR010000447 DAOQTR010000000
VERSION     DAOQTR010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75147_length_1954_cov_3.238547"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKN85_09720"
     CDS             <1..633
                     /locus_tag="PKN85_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT44731.1"
                     /translation="IATFLVYMLISGNAFLMPFYLEVVKGLNAQAAGMMLLIYSIIYV
                     ILSPVSGRLSDRVNPALLCTIAMTSATVCVLVFSFALRLQGLTIVVVYLVWLALSLVL
                     FFSPNNNQIMRFAPPDKKGVSSGLFNTTTNLGMVVGVTVIEVVFSAVAYDTAGPSGKA
                     ALKDLVGTGSVTSGFTHAYLTGALCCLLALVFSLLGRKGGGDGGRGSKKA"
     gene            complement(643..>1954)
                     /locus_tag="PKN85_09725"
     CDS             complement(643..>1954)
                     /locus_tag="PKN85_09725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT44732.1"
                     /translation="YQLIVDPITAGIRIIVKDKGLPYSPHLVPQYVPPSDIETAGQPG
                     LGSHLIKQTVDEIHFQNMGREGKELHLVKYLPYRSIEEIRTESELAPFPEPVKTVAPP
                     EKKDFSIRRIRPSEVYDVSKLFYRAYGYSYGIDTIYYPEKLAERHADGTIISVVTVTP
                     DERVVGHAALVRDDASSKTAEAAMAVVEPGFRGQGCQSIMITKLVEEARAVGLAGIYS
                     KAVTNHIYAQKAGQKAGFKRCAVVAGLIPADRSFKGIQAALSQRESVAYGYRVVDDPE
                     NMEIFPPAWHRDIIERIYDSTGVRRIFCESPPGAMHDLAGEAEVMVTVVPTYQRAVIE
                     VKRYGEHTVSQVSAILKDLCYQKMEQITLYLNLEDPITGIMCRRFEELGFFFAGILPF
                     SHVGDALLLQYLNNVPIDYSKIKIVDEMGQEILSYVESHDPSRK"
BASE COUNT          349 a          568 c          559 g          478 t
ORIGIN      
        1 atcgccacct ttctcgtgta catgctcatc tcgggaaacg cctttctcat gcctttttac
       61 ctggaggtgg tcaaggggct caacgcccag gccgccggca tgatgctcct catctactcc
      121 atcatatatg tcatcctgtc gcccgtatcg ggcagacttt ccgacagggt aaaccccgcc
      181 ctcctgtgta ctatcgccat gacctcggcg acggtgtgcg ttcttgtgtt ttcttttgcc
      241 ctccgcctgc aaggtctcac gatcgttgtc gtctacctgg tgtggcttgc gctttccttg
      301 gtcctctttt tttcacccaa caacaaccag atcatgcgct tcgcgccgcc tgacaagaaa
      361 ggcgtctctt cggggctttt caatacgacg acgaaccttg gaatggtggt gggcgtgacg
      421 gtcatagaag tggtcttctc cgccgttgct tacgataccg cgggcccgtc cgggaaggca
      481 gcgttgaaag acctcgtcgg cacggggtcc gtcacctcgg gttttactca cgcgtacctt
      541 accggagcgt tgtgttgtct cttggcgctc gttttctcgc ttctgggtag aaagggcggc
      601 ggtgatggcg gccggggatc gaaaaaagca tagcgttgaa cgtcacttcc tgctcgggtc
      661 gtgggattcg acgtatgaaa ggatctcctg acccatctcg tcaacgatct ttatcttact
      721 gtaatcgatg ggcacgttgt tgagatactg gaggagcaag gcgtctccca cgtgggaaaa
      781 cgggaggatc ccggcgaaga agaagcccag ttcttcgaac ctccggcaca tgatcccggt
      841 gatggggtcc tcgaggttaa ggtagagagt gatctgttcc atcttctgat aacagagatc
      901 tttcaagatg gcgctcacct gcgatactgt gtgttccccg taccgtttga cctcgatgac
      961 ggccctctga tacgtgggaa cgaccgtcac catcacctcc gcctctcccg caaggtcatg
     1021 catcgctccc ggaggggatt cacaaaagat cctcctcaca cctgtagagt cgtatatcct
     1081 ttctattatg tctctgtgcc aggcgggggg aaatatctcc atgttttccg ggtcatcgac
     1141 caccctgtac ccgtacgcga cggactcccg ctgtgaaagt gccgcctgta tgcccttgaa
     1201 ggatcggtcg gcggggataa ggcccgcgac caccgcgcac cgcttgaagc cggctttctg
     1261 accggccttc tgggcgtata tgtggttcgt gaccgccttg ctgtatatgc ccgccagtcc
     1321 gacggcacgc gcctcttcaa cgagctttgt aatcatgatg ctctggcatc cctgacccct
     1381 gaagcccggc tcgacgacgg ccattgccgc ctcggcggtc ttgctcgacg cgtcgtccct
     1441 gacgagggcc gcatggccga cgacacgctc atcgggtgtc actgtcacca cggaaatgat
     1501 ggtgccgtca gcatggcgtt cggccagctt ctcggggtag tagatcgtgt cgatgccata
     1561 ggaataacca taggccctgt agaagagttt cgacacatcg tagacctcgg agggccgtat
     1621 gcgccggatg gagaagtcct tcttctccgg aggtgccacc gtcttgacgg gctccgggaa
     1681 cggggccagt tcggattctg tccgtatctc ctctatgctt ctgtagggga ggtacttgac
     1741 gaggtgcagt tcctttcctt cgcggcccat gttctggaag tgtatctcgt cgacggtttg
     1801 cttgatgaga tgcgagccga gtccgggctg cccggccgtc tcgatgtcgg aaggcggaac
     1861 atactggggc acgagatggg gcgaataggg cagccccttg tccttgacga tgatcctgat
     1921 ccccgcggtg atcgggtcga cgatcagctg atat
//
LOCUS       DAOQTR010000448         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_75788_length_1944_cov_2.500794, whole genome shotgun sequence.
ACCESSION   DAOQTR010000448 DAOQTR010000000
VERSION     DAOQTR010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75788_length_1944_cov_2.500794"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKN85_09730"
     CDS             <1..340
                     /locus_tag="PKN85_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44733.1"
                     /translation="EEQWERERRRREFSERVEEANRNLREQKEKEERRIQEEAEQYRS
                     RMRDQMEARRKALSECLQTANYRYQTAWNDSCKSVNLGAQCKLPYSSASILEQRLMQS
                     RNECFRLYPQ"
     gene            complement(348..>1944)
                     /locus_tag="PKN85_09735"
     CDS             complement(348..>1944)
                     /locus_tag="PKN85_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNT44734.1"
                     /translation="AETKSSPKPTVRTEEKTSTAYPVTLGNQILFSFRDIKGLPAEKR
                     AKAFSDRLKMIAEDLSIHPDNIKISDFSQPLTVATADDVMLFAILDEDAKREGTTRRE
                     LATERAQIIRKAIEKYRHDYGRKQLILGIVYSLLATAVLLAVLYLVVRAYRALTGKVD
                     EWTGAKIKKLHMESLPFIRAKRVTALLAGSLKIARFAVFVVLLYSYAHLLLRFFPWTR
                     PLAGELTDYIMMPLQIIGRTVWRELPNLLFVAIIVFFTVVVLKLMRSVFTGLENGTIT
                     IQGFYPEWSLSTYKIFRILVIAFAAVVAFPYIPGSSSAAFKGISLFLGVLFSLGSTST
                     IANILAGYSLTYRRIFKIGDRVKIGDFVGDVVDMRLQVVSLRTIKNEVIVVPSSMIVN
                     TNVLNYSTLAKEQGLILHTKVTIGYDAPWRQVEALLLLAAGKTEGVLRQPAPFVVQTS
                     LDDYYVSYELNVYTERPETMVKVYTDLHRNIQDAFNEYGVQIMSPAYRGDPSQPKVVP
                     MDQWYAPPALNADDRDKEPGVKG"
BASE COUNT          432 a          516 c          574 g          422 t
ORIGIN      
        1 cgaggaacag tgggagcgcg agcggcggcg gagagagttc tcggagcggg tcgaagaggc
       61 caaccggaac ctcagggagc aaaaggagaa ggaggagcgc cggatccagg aggaggccga
      121 gcagtacagg tcccgaatgc gcgaccagat ggaggcgagg cgcaaggccc tctccgaatg
      181 cctccagacc gcgaactatc gctatcagac ggcgtggaac gactcctgca agtcggtgaa
      241 tctcggtgcg cagtgcaagc ttccttattc ttcggccagc atactggagc agcgcctcat
      301 gcagtcccga aacgaatgtt tccggcttta tccgcagtaa cagggtgtca tcctttaacc
      361 cccggttcct tgtcccgatc gtcggcgttc agggcggggg gcgcatacca ctggtccatt
      421 ggaaccactt ttggctgcga tgggtcgccg cgatatgcgg gcgacatgat ctgtaccccg
      481 tattcgttaa aggcgtcctg aatgttccgg tgaaggtcag tatagacctt caccatggtc
      541 tcggggcgtt ccgtgtatac gttgagttcg tagctcacat aatagtcgtc gagggatgtt
      601 tggacgacaa agggagcggg ctggcgcaat accccttccg ttttgcctgc ggcgagaagg
      661 agtagcgcct cgacctgcct ccagggggcg tcatagccga tggtaacttt tgtatggagt
      721 atcaggccct gttcctttgc aagcgtgctg tagttcagga cgttggtgtt gacgatcatt
      781 gaggagggca caactatcac ctcgttcttg atcgttctga ggctcaccac ctggagcctc
      841 atatccacga cgtccccgac gaagtcccct atctttaccc ggtcgccgat tttgaagatt
      901 ctgcgatagg tcagtgaata tccggccagg atgttggcga tcgtcgaggt tgaaccgaga
      961 gagaagagga cacccagaaa aagtgaaatc cccttgaagg cagcggaact ggaaccggga
     1021 atatagggaa aggcaacaac cgccgcaaag gcgataacga ggatgcggaa tatcttgtac
     1081 gtcgacagcg accactcggg atagaatccc tggattgtta ttgtcccgtt ctcgagcccg
     1141 gtgaacacgg acctcatcag cttcagtacc acgacggtaa agaagactat gatagccacg
     1201 aaaagcaggt tcggaagctc ccgccagacc gtcctcccta tgatctgcag gggcatcatg
     1261 atgtagtcgg tcagttctcc cgccagcggc cttgtccagg ggaagaacct cagaagcagg
     1321 tgcgcatagg aatagagaag gacaacaaat accgcgaagc gcgcgatctt gagggatccg
     1381 gcgagaagcg cagtcaccct ttttgcccga atgaacggca gagactccat gtggagcttc
     1441 ttgatctttg ccccggtcca ttcatccacc ttccctgtga gggcgcggta cgcccttacg
     1501 acgagataca ggaccgccag gagaactgcc gttgccagca gggagtagac aatgccgagg
     1561 atcagctgct tccggccata atcatgacga tacttctcta ttgctttgcg aattatctga
     1621 gcgcgctcgg ttgcgagttc acgccgcgtc gttccctcac gtttcgcatc ctcgtcgagg
     1681 atggcaaaga gcatgacatc gtcggcagtt gccacggtca gaggttgact aaagtcggat
     1741 atctttatgt tgtcagggtg aatgctcagg tcctcggcga tcatcttcaa tcgatccgag
     1801 aacgcctttg cccgtttttc cgccgggagc cccttgatgt ccctgaaaga gaacagtatc
     1861 tgattgccga gcgtgaccgg gtaggctgtg gaagttttct cctcagttct gacagttggt
     1921 ttcggggagg actttgtttc tgcc
//
LOCUS       DAOQTR010000449         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_75830_length_1943_cov_3.243644, whole genome shotgun sequence.
ACCESSION   DAOQTR010000449 DAOQTR010000000
VERSION     DAOQTR010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75830_length_1943_cov_3.243644"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1199
                     /locus_tag="PKN85_09740"
     CDS             123..1199
                     /locus_tag="PKN85_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT44735.1"
                     /translation="MLPIQKILCPTDFSRHSQDAIEVARELAAYFEAELILVNVAPDV
                     PTLAYAPVPTNFDVGEYGRELVRSSESTLEEIVESTKGDDLKIRPVVLQGNPSDQITR
                     EAQKDMVELIVISTHGRTGIDRLVFGSVTERVVRFAPCPVLVIPMRPGEEVEEAATEP
                     IAGSHVDDVLESQLAELGEMLDAIKRRIEDRKGSIDSMYQRRLAELREKSEEVQNRMG
                     RIRESGGEAWGEIKTGFGDLWAAFDRAFSKFREKKEGATENIAEEKKVYEERLGAMLD
                     EWGSKIDALKEQIEMSKGTSRDLYVRYVGDLKEKQRVAGEKLAEFRRSGSDAWGTMKK
                     GVDKAVEDLGEAIREAVSAFKGRG"
     gene            1205..>1943
                     /locus_tag="PKN85_09745"
     CDS             1205..>1943
                     /locus_tag="PKN85_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT44736.1"
                     /translation="MRIVVFELDTWERTFFEKFSSAYDVRFVEESLTADNVEAYADAE
                     IISPFIYSTLNGDVLGRMVDLKLIATRSTGYDHIDIDHCSSRGITVSNVPSYGECPVA
                     EHVFALLLAVARRIVSAADRTRRGDFSLQGLQGFDLCGRTMGVVGTGNIGRRVMEIAR
                     GFGMDVVAFDLKPDTGFAQSIGARYASMDELLSTADVITLHVPGTPATENMVSEKQFA
                     MMKKGVVLINTSRGSVIDVRALLHALSE"
BASE COUNT          429 a          493 c          655 g          366 t
ORIGIN      
        1 accgggagag gtcagccctg aggaagagcg cggagacgtt gcgcctggcg atcgatgacc
       61 tcgagcgcgc ggtgtaatga aagaggctca tgcagcagtc aagaaagaaa ggaggtttga
      121 gaatgttgcc gatccagaag atactttgtc ccacggattt cagtcgacac tcgcaggatg
      181 ccatcgaggt ggcgcgggaa ctcgcggctt actttgaagc agagctcatt ctggtgaatg
      241 tcgcccccga tgtcccaacc ctggcatatg ccccggtgcc caccaatttt gacgtcggcg
      301 agtacggcag ggaacttgtc aggtcctcgg aaagcaccct tgaggagatc gtcgagtcca
      361 caaagggcga cgatctgaag atccgtccgg tggtcctgca gggaaatccc tccgatcaga
      421 tcacgagaga ggcgcagaag gacatggtgg agctcatcgt catatccacg catggcagga
      481 cgggtatcga ccggctggtc ttcggttccg tgacggagag ggtggtccgc ttcgcgccct
      541 gccccgtgct ggtcatcccc atgcgtccgg gagaagaggt ggaggaggcg gcaacggagc
      601 cgattgcggg gtcccacgtc gacgatgtcc tggagtctca gctcgcggag ctcggtgaga
      661 tgctggacgc catcaaaagg aggatagagg ataggaaggg gagcatagac tccatgtatc
      721 agcgtcgcct cgccgaactc agggaaaaga gcgaagaggt gcagaacagg atgggccgca
      781 tccgcgaatc gggcggagag gcctggggag agatcaagac gggtttcggt gacctctggg
      841 ccgcctttga cagggccttc tcaaaattca gggaaaagaa ggagggagcg acggagaaca
      901 tcgcggagga gaaaaaggtc tacgaggaac ggttgggcgc catgctcgac gaatggggtt
      961 caaagataga cgcgctcaag gagcagatag agatgtcgaa gggcacctcc cgggaccttt
     1021 acgtcaggta cgtcggggac ctgaaagaga agcagcgggt tgccggtgag aaactcgccg
     1081 agttcaggcg gtccggctcc gacgcctggg gtaccatgaa gaagggcgtc gacaaggccg
     1141 ttgaagacct gggggaggct atcagggaag cggtgtcggc cttcaagggc agggggtgag
     1201 gataatgaga atagttgttt tcgagctcga cacgtgggaa aggaccttct tcgagaaatt
     1261 ttcctctgca tatgacgtca ggttcgtcga agaatcgttg acggctgaca acgtggaggc
     1321 ttacgcggac gcggagataa tatcgccttt catctattcg acccttaacg gcgacgtcct
     1381 gggcaggatg gtcgacctca agctgatcgc cacgcggtcg acgggctacg atcacatcga
     1441 catcgatcat tgctcgtctc gcggaattac ggtgagcaat gtgcccagct atggagaatg
     1501 tcccgtggcg gagcatgttt tcgccctttt gcttgccgtt gcccgccgga tagtcagtgc
     1561 cgcggacagg acgaggaggg gggatttttc tctccagggt ctgcagggat tcgatctgtg
     1621 cggcaggacc atgggtgttg tgggcacagg gaacatcggt cgccgggtga tggagatcgc
     1681 caggggattc ggcatggatg tcgtggcctt cgatctcaag ccggacacgg ggtttgctca
     1741 gagcatcggg gccaggtacg cgtccatgga cgagcttctt tccaccgccg atgtcattac
     1801 cctgcacgtg cccggcacgc ctgcaacgga aaacatggtc tccgagaagc agttcgccat
     1861 gatgaagaag ggggtcgttc tgatcaatac ctcgcgcggc agcgtgatcg acgttcgcgc
     1921 cctgctccac gccctttccg aag
//
LOCUS       DAOQTR010000450         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_75857_length_1943_cov_2.460805, whole genome shotgun sequence.
ACCESSION   DAOQTR010000450 DAOQTR010000000
VERSION     DAOQTR010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75857_length_1943_cov_2.460805"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKN85_09750"
     CDS             <1..180
                     /locus_tag="PKN85_09750"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HNT44737.1"
                     /translation="EDEGLKEDLLYTIDGRGAFRRFKDAIHRLGIEKDWFKYRDGRLK
                     ELAVDWLKANGIPFI"
     gene            complement(308..475)
                     /locus_tag="PKN85_09755"
     CDS             complement(308..475)
                     /locus_tag="PKN85_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44738.1"
                     /translation="MEEKDSKKIPEHNCNEHVRYDGGQKINRDGVRIWVETYYCRICG
                     RRLDIKEEILD"
     gene            complement(492..>1943)
                     /locus_tag="PKN85_09760"
     CDS             complement(492..>1943)
                     /locus_tag="PKN85_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44739.1"
                     /translation="LLVALRKANGALFNFYVEIALSLIHVHEDHGVPALAKRPSRDGA
                     EPPMGAVPVADLGPAPCIREAQIAILARHARGLENHHGVRIGADVLSEAWHATQRHLS
                     NRTQPDKSVDLLDSAAGLVEREGRNEMGTEDLYRVLSRQTGRMMSALGEGRTRDLTGL
                     EDSLNRQVIGQEEAVRRVVSTLVSRRYGEVAPVRPLGTFLFSGATGVGKTELARTLAR
                     EYFGSPKDFLHIDLAEYSGQDGVHKLIGMPYGSAFGTADGLLTRWLEERSSGVILFDE
                     IEKAHPDVRTLLLGMLDEGRVTSGMSERFDIRRCVIILTTNVLAHRDVDRVKLGFSTG
                     DDPSDPFALLAKHFPEEFLGRLDEIIVFRTPPAPVLRRIMAQKLREALARLKERGVFL
                     IYDEDRLLDHLLAGTGRPWAGARDIARLIERKLIQPIARRLIDCDTQDWICIELTESF
                     YRSGALNPLPLLKRPGPPPDRPDTPASAADARQ"
BASE COUNT          339 a          582 c          622 g          400 t
ORIGIN      
        1 gaggacgagg gcctgaagga agacctcctc tacaccatcg acggccgcgg agccttccgc
       61 cggttcaagg acgccattca ccgcctgggg atcgaaaaag actggttcaa atatcgcgat
      121 gggaggctga aggagctagc ggtcgactgg ctgaaagcca acgggatccc cttcatttga
      181 actccgggga catcccttca cattttcaca ttctcaaaat actgcaagta cggagactgc
      241 cagatagcct ttccatcgtc tttcggttct aacgttcaac agcggttcaa gccattcaaa
      301 ccgtccctca gtccagtatc tcttctttta tatccagacg cctcccgcag atgcggcagt
      361 aatatgtctc aacccatatt cgcacgccgt ctcggttgat cttctggcca ccatcgtagc
      421 ggacatgttc gttgcagttg tgttccggga tcttcttgct gtctttctct tccatgatgg
      481 tacctccttt gttattgccg ggcatccgcg gcggaagccg gggtgtcggg gcgatccggc
      541 ggtgggccgg ggcgtttgag gagcggcagc gggttcaggg cccccgaacg gtagaaggac
      601 tccgtgagct cgatgcagat ccaatcctgc gtgtcgcagt cgatgaggcg gcgggcgatg
      661 ggctggatga gctttcgctc gatgaggcgg gctatgtcgc gcgcgccggc ccagggtctt
      721 ccggtgcctg cgagaagatg gtcgaggaga cggtcctcgt catagatgag gaagaccccc
      781 ctttccttga ggcgggcgag ggcctctcgc agcttctggg ccatgatcct cctcaggacg
      841 ggcgccggcg gcgtcctgaa gacgatgatc tcgtcgagcc ttcccaggaa ctcctcaggg
      901 aagtgtttcg cgaggagcgc gaaggggtct gacgggtcgt cgcccgtgga gaaccccagt
      961 ttcacccggt cgacgtcgcg gtgggcgagg acgttcgtgg tgaggatgat gacgcagcgg
     1021 cggatgtcga agcgctctga catgcccgac gtcacccttc cttcgtccag cattcccagg
     1081 agaagcgtgc ggacgtcagg gtgcgccttt tcgatctcgt caaagaggat aacaccgctt
     1141 gacctctcct cgagccaccg ggtgaggagg ccgtcggccg tcccgaaggc ggagccgtag
     1201 ggcattccga tgagcttgtg cacgccgtcc tgcccagaat actcggcgag gtcgatgtgg
     1261 aggaagtcct tcgggcttcc gaaatactcc cgcgccaggg tccgggcaag ctccgttttg
     1321 ccgacgcccg tggcgccgga gaagaggaag gtcccgagcg gtctcaccgg ggcaacctcc
     1381 ccgtaccttc gcgaaacgag ggtcgaaacg accctgcgta ccgcctcctc ctggccgatg
     1441 acctgcctgt tgagagaatc ctcgaggccc gtcaggtccc tcgttctgcc ctctccgagg
     1501 gcgctcatca tccttcccgt ctggcgtgag aggacgcggt agaggtcctc ggttcccatc
     1561 tcattcctgc cttccctttc gacgagaccc gcggcgctgt cgagaaggtc cacgctcttg
     1621 tcgggctggg tccggttcga caggtggcgc tgtgtggcgt gccaggcctc ggagagaaca
     1681 tcggccccga tccggacccc gtggtggttc tcgaggcctc gcgcgtggcg cgcgaggatg
     1741 gcgatttgtg cttctctgat gcagggagcg ggaccgagat cagctaccgg aacggcgccc
     1801 attggcggct cggcgccatc gcgggatggg cgcttggcga gggccggaac tccatgatct
     1861 tcgtgtacgt gaatgagact aagcgcaatt tcgacgtaga agttgaacag ggcgccgtta
     1921 gcttttctca gcgcgacgag cag
//
LOCUS       DAOQTR010000451         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_76092_length_1939_cov_3.953822, whole genome shotgun sequence.
ACCESSION   DAOQTR010000451 DAOQTR010000000
VERSION     DAOQTR010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76092_length_1939_cov_3.953822"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PKN85_09765"
     CDS             complement(<1..1318)
                     /locus_tag="PKN85_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNT44740.1"
                     /translation="MFFRRLKGTGKTCAVAALVLLCLVAAGLLAVPRSVRNTDAGSFQ
                     ALKASYRTSDAVLLDRRGEVIHTLRIDDSGRRLEWARLADVSPSFLSTVILVEDRRFF
                     SHHGVDPFAFISALARGAAGGKLRGASTISMQVASMVDRDLRPKGMRRDLGQKWRQII
                     TALAMERSWTKEEILEAYINLVYYRGELQGVRAASKGLFGKEPSGLTQLESVVLASLI
                     TSPNAPREKVAARAWRLALRAGMRITREEVGRKAADALASAYRIAFDASLAPHVARML
                     LAGGRLKMATSLDGRLQRFVLDALRDNLAALRGRNVADGAVMVVDNATGEVLAYVGNS
                     GSTSSAVWVDGIRAHRQAGSTLKPFLYGLAIEKGYLTASSLLEDTPLSIMTPTGLYVP
                     QNYDNQFRGTVSVRCALASSLNVPAVKTLLVTGLEPFVERLKALGFA"
     gene            complement(1323..>1939)
                     /locus_tag="PKN85_09770"
     CDS             complement(1323..>1939)
                     /locus_tag="PKN85_09770"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44741.1"
                     /translation="GTSSVTLGGKQDSLSWAENAKGGSLMLPWPGKRSVLTIDHRGEG
                     RPWASVQSLAAVPLKAPLASGYTIKKTITAVERKSPDVWTRGDVMRVQLQIDARSDMT
                     WVVVSDPIPSGAAILGSGLGQDSALLSRQGRQRGWAWEAYRERSFEALRVYYEFVPRG
                     KWTVEYSVRLNNDGTFRLPETRVEALYAPEMFGEIPNPPVEVRK"
BASE COUNT          357 a          640 c          591 g          351 t
ORIGIN      
        1 tcgcgaaccc cagagccttg agtctttcga cgaagggctc gaggcccgtc acgagaaggg
       61 tcttgacggc gggcacgttg agggacgatg caagggcgca cctcacactc acggtgccgc
      121 ggaactggtt gtcataattc tgcggcacgt agagccctgt cggcgtcatt atggagaggg
      181 gcgtgtcctc gagaagggat gacgcggtga gatagccctt ctctatggca agcccgtaaa
      241 ggaagggttt cagcgtcgaa cccgcctgtc tgtgcgcccg gatgccgtcg acccacacag
      301 cgctcgatgt gctcccgctg ttgccgacgt aggcaaggac ctcgcccgtg gcattgtcga
      361 cgaccatgac ggcgccatcg gccacgttcc gcccacgcaa cgcggcaagg ttgtccctga
      421 gcgcatcgag gacgaacctc tggagccgcc catccaggct ggtcgccatc ttcagccttc
      481 caccggcgag gagcatgcgt gccacatggg gcgcgaggga cgcgtcgaag gctatgcggt
      541 atgccgatgc cagggcatcc gccgctttcc ttccaacctc ctcgcgggtg atcctcatgc
      601 cggccctcaa cgccagtcgc caggccctcg cggccacctt ttccctgggc gcgttgggcg
      661 atgttatgag agaggccaga acgacggact ccaactgggt gaggcccgac ggttcctttc
      721 cgaagagacc cttcgacgcc gctctgacac cctggagttc tccgcggtag tatacgagat
      781 tgatgtaggc ctcaagtatc tcctccttcg tccaggagcg ttccatggca agggcggtga
      841 tgatctgccg ccatttctga ccgagatctc tgcgcatccc tttcggtctc aggtctctgt
      901 ccaccatcga ggcgacctgc atcgagatgg tgctggcacc ccggagcttt ccacccgcgg
      961 caccgcgcgc gagggcggaa atgaaggcga aggggtccac cccgtgatga ctgaagaaac
     1021 ggcgatcctc aacaagaata acggtggaga ggaaggatgg cgagacatcg gcaagacggg
     1081 cccactccag ccgcctgccg ctgtcatcga tcctcagtgt gtggatcacc tcgccgcgac
     1141 gatcgagaag gacggcatcg gaggtgcgat acgaagcttt aagggcctga aaggaccccg
     1201 cgtcggtgtt cctgaccgac cggggcaccg cgaggagccc ggcggcgaca aggcacagga
     1261 ggacaagagc cgcgacggcg catgtcttcc cggtgccttt cagccgtctg aagaacatcc
     1321 tgctatttcc tcacctccac gggcggattc gggatctctc cgaacatctc cggcgcgtag
     1381 agcgcctcca cccgtgtctc gggaagacgg aacgtaccgt cattgttgag cctcacgctg
     1441 tactccacgg tccacttgcc gcgggggacg aactcatagt acaccctcag ggcctcgaag
     1501 gaccgttcac ggtaagcctc ccacgcccat ccccgctgac gtccctggcg ggaaaggagg
     1561 gcggaatcct gcccgagacc gcttcccagg atggcagcgc ccgatgggat gggatcgctg
     1621 acgaccaccc aggtcatgtc cgagcgggca tcgatctgaa gctgcacgcg catcacatcc
     1681 ccgcgtgtcc acacatccgg cgacttgcgc tccaccgccg ttatcgtctt ctttatggtg
     1741 tagcctgaag ccagcggcgc cttcagcgga accgccgcca ggctttgcac gctagcccag
     1801 ggccgtccct cgcccctgtg gtcgatcgta aggacgctcc tctttccggg ccagggcagc
     1861 atgaggctgc ctcccttcgc gttctcagcc cacgacaggc tgtcctgctt cccgccgaga
     1921 gttacggaag atgtcccct
//
LOCUS       DAOQTR010000452         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_76098_length_1939_cov_3.602972, whole genome shotgun sequence.
ACCESSION   DAOQTR010000452 DAOQTR010000000
VERSION     DAOQTR010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76098_length_1939_cov_3.602972"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PKN85_09775"
     CDS             <1..681
                     /locus_tag="PKN85_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HNT44742.1"
                     /translation="QVIDPQKASYGINNYQFAATQLAQTTMRSVIGKLELDRTFEERD
                     TINTTIVEAVDKASDPWGVKVTRYEVKNIVPPQSIKDAMEKQMRAEREKRAMIAESEG
                     DKQAKINRAEGVKQEMIATSEGEKQKRINEAEGRAAEIERVAKATANGLREIALAINE
                     QGGINAVNLRIAEQYINEFGKLARTNNTLILPANLGDLAGLVSTVTTILKDQTKDTGV
                     RAQGAEKR"
     gene            857..1294
                     /locus_tag="PKN85_09780"
     CDS             857..1294
                     /locus_tag="PKN85_09780"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNT44743.1"
                     /translation="MKVSILAALVIVLALVWCGGVQAAEPNMKDGLWEIKTKVEMKGM
                     PASIPPSVTKQCLTKKDSVPGQGKEKNPNCKITDQKVSGNTVTWAMVCKEKDGTVESK
                     GSITYKGDTFDGTTTTLIKNKGMQAQQVSTKMSGKRLGPCDKK"
     gene            1318..>1939
                     /locus_tag="PKN85_09785"
     CDS             1318..>1939
                     /locus_tag="PKN85_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44744.1"
                     /translation="MKIKIRKSRKTTINEVDFDNLEFGVHLSDHMYYVDYADGHWSEP
                     RIEPYGPMKMYPALSTLHYGQAVFEGLKAFLGVDGKIRLFRPDKNHERYNVSCERLCI
                     PTLSWEMFFEGMRKLVAIDRAWIPVKRGCSLYIRPFVFATDNFLGVRVSHTYRFMIIT
                     SPVGAYYKEGLNPVKLITSGEYVRAVRGGLGAVKTPANYAASLYPGE"
BASE COUNT          517 a          542 c          571 g          309 t
ORIGIN      
        1 caggtcatag atccccagaa ggcgtcttac ggcatcaaca attaccagtt cgcggccact
       61 cagctcgccc agacgaccat gaggagcgtc atcggcaaac tggagctcga caggaccttc
      121 gaggagcggg acacgatcaa caccaccatc gtcgaggccg tcgacaaggc gtccgatccc
      181 tggggcgtga aggtcacccg gtacgaggtc aagaacatcg ttccgccgca gagcatcaag
      241 gacgccatgg agaaacagat gcgcgcggag cgtgagaaga gggccatgat cgctgaatcc
      301 gagggagaca agcaggccaa gatcaaccgc gccgagggtg tcaagcagga gatgatcgcc
      361 acctccgaag gcgagaagca gaagaggatc aatgaggccg aaggccgggc ggcggagata
      421 gagcgcgtgg caaaggcaac cgcgaacggc ttgagggaga tcgcgctcgc tatcaacgag
      481 cagggcggca tcaacgccgt caaccttcgc atcgccgagc agtacataaa cgagttcggc
      541 aagctggcga gaacgaacaa caccctcatc ctccccgcca accttggcga cctggcaggc
      601 ctcgtatcga cggtaacgac aatactgaag gaccagacaa aggatacagg ggtcagggcc
      661 cagggggcgg agaaaagata gacgatagcg gggcagcata cacgacgatc gccctggaca
      721 agaccgtcgg aaccgcacat ttttctgact ctcaagcggt tcaagcgact caagccgtct
      781 ttctcttgac ccatcggccc cattagtgat accttttttt accgaagcat ttgtcaggaa
      841 aagatcagga ggacatatga aagtgagcat tcttgcagca ctcgtcatcg ttctggctct
      901 cgtgtggtgc ggcggcgtgc aggccgcgga accgaacatg aaggatggtc tctgggagat
      961 caagacgaag gtggaaatga agggcatgcc cgcgagcatc cccccgtccg taacgaagca
     1021 atgcctcacg aagaaggaca gcgtgcccgg gcagggcaag gagaaaaacc cgaactgcaa
     1081 gatcaccgac cagaaagtga gcggcaacac ggtgacgtgg gccatggtct gcaaggaaaa
     1141 ggacggcacc gtggaaagca agggaagcat cacatacaag ggcgacacct ttgacgggac
     1201 aacaacgacg ctcatcaaga acaaaggcat gcaggcccag caggtttcga caaaaatgag
     1261 cggcaaacgc ctcggaccct gcgacaagaa ataggaaaga acgcgaggaa gagagctgtg
     1321 aagatcaaga taaggaagag cagaaaaaca acgatcaacg aagtggactt cgacaacctg
     1381 gagttcggcg tccatctctc cgatcacatg tactacgtgg actacgcgga tggacactgg
     1441 agcgagccac gcatcgagcc ttatggcccg atgaagatgt acccggccct ttccacgctg
     1501 cattacggcc aggcggtctt tgaaggcctc aaggcctttc tcggagtgga cggaaagatc
     1561 cgactttttc gcccggacaa gaaccatgag agatacaacg tatcctgtga acgcctgtgc
     1621 atccccactc tctcgtggga aatgttcttc gaggggatga gaaagctcgt ggcgatcgac
     1681 agggcatgga tccccgtcaa gcgcggctgc tctctctata tccggccttt cgtcttcgcg
     1741 acggacaact tcctcggcgt cagggtatct cacacgtacc gcttcatgat cataacctct
     1801 cccgtcggtg cctattacaa ggaagggctc aatccggtaa agctcatcac ctccggcgag
     1861 tatgtccggg ccgtgagagg cggcctcggc gccgtcaaga cgccggcaaa ctacgccgct
     1921 tcgctctatc ccggggaga
//
LOCUS       DAOQTR010000453         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_76754_length_1929_cov_2.595518, whole genome shotgun sequence.
ACCESSION   DAOQTR010000453 DAOQTR010000000
VERSION     DAOQTR010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76754_length_1929_cov_2.595518"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..814)
                     /locus_tag="PKN85_09790"
     CDS             complement(104..814)
                     /locus_tag="PKN85_09790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT44745.1"
                     /translation="MAADDKKSLIVLREKHQVDQYIREITGQSMPVRVVIDDTIVLDD
                     CRLVMREGKRRDLFVEASLAPGEYPEAGRATITCAGDSSLYTFGTKVLSIELAEHRRM
                     HLTIQYPDRITKRERRRHVRVRPSANRPVSARIVIDNDEVVQVEPVDISTGGISFMMT
                     EEMSRFKSGDAVELVITVPAFGEVRAGATVRSVVHLMDLTRIGVEFSSLPDDALRLIT
                     EYVSERERQTGLAVFEDN"
     gene            937..1479
                     /locus_tag="PKN85_09795"
     CDS             937..1479
                     /locus_tag="PKN85_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNT44746.1"
                     /translation="MMNRALILFLLLAAILFPPPGAGCAEYGKDVTVSLCFTPGGSCT
                     DRIIREISSARSEILIQAYSFTSAPIAKALIEAHKRGVKVIAILDKSQRTAKYTSATF
                     LANMRIPTYIDSRHAIAHNKVIIIDGAVTITGSFNFTKAAQEKNAENLLIIRSREIAR
                     AYRENWQHHLSHSERYEPKY"
     gene            1619..>1929
                     /locus_tag="PKN85_09800"
     CDS             1619..>1929
                     /locus_tag="PKN85_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT44747.1"
                     /translation="MITVFIDDKECSAREGSTIIDAARENGIDIPSLGYDPRVSPPTN
                     IEISMVEVVENGRPRTVSATSTVMEDGMVITTRSPALESFRKIYLQALLRNHYGDCVA
                     P"
BASE COUNT          403 a          633 c          488 g          405 t
ORIGIN      
        1 cgcgacgagt caagtgcgcc ccttcatcgg gcggcaccgg ttaacgtttg gcgagggaga
       61 agcggtcttc cgccaggagt tcctttttga ggaagctgcc tcttcagttg tcctcgaaca
      121 ccgcgaggcc cgtctggcgt tcccgctcgg acacgtattc cgttatgagc cttaaggcat
      181 cgtcgggcag ggaggagaac tcgaccccga tcctggtcag gtccatgaga tggacgacgc
      241 tcctcaccgt ggcccctgcg cgcacctctc cgaacgcggg aaccgtgatg acaagctcga
      301 cggcgtcgcc cgacttgaaa cggctcatct cttccgtcat catgaaggat atgccaccgg
      361 tacttatgtc taccggttcc acctggacga cctcatcgtt gtcgatgacg atccgtgcag
      421 acaccgggcg gtttgccgac ggacggaccc tcacgtgcct gcgtctttcc cgctttgtga
      481 tcctgtcagg gtactgaatg gtcaggtgca tgcggcgatg ttcggcaagc tctatggaaa
      541 ggaccttcgt cccgaaggta taaagggagc tgtcgcccgc gcaggtaatg gtggcccgtc
      601 ccgcctccgg gtattcccct ggcgccagcg atgcctcgac aaagagatct ctcctcttcc
      661 cttcccgcat cacaagccgg caatcatcaa ggacgatggt gtcatcgatg acgaccctga
      721 caggcatgct ctgcccggtt atctcccgga tgtactggtc tacctgatgt ttctcgcgaa
      781 gaacgatgag actctttttg tcgtcagctg ccatgatatc acacggtctc gatctttatc
      841 ctgttaattc tctttcaatt gctccccgtt tacaagagaa aagtgcgatt gacttttccc
      901 gtgccttcat ctacgctatg tgcgaaacca gcgctcatga tgaacagggc cctcattctc
      961 ttccttctcc tcgccgccat tttgtttccg cccccaggcg ccgggtgcgc ggagtacggc
     1021 aaggacgtca ccgtcagcct ctgcttcacc cccggcggtt cctgcacgga caggatcata
     1081 cgggagatct cctctgcgcg ctcagagatc ctcatccagg cgtactcctt cacctccgcc
     1141 cccatcgcga aggccctcat cgaggcgcac aagcgcggcg tcaaggtcat cgcgatcctc
     1201 gacaagagcc agcgcacggc aaaatacacc tccgccacat tcctggcgaa catgaggata
     1261 cccacataca tcgactcccg gcacgccatc gcccacaaca aggtcatcat catagacggc
     1321 gcggtaacga taacgggctc cttcaatttc acgaaggccg cccaggaaaa gaacgccgaa
     1381 aacctcctca tcatccgttc ccgggagatc gccagggcct accgcgaaaa ctggcaacac
     1441 cacctcagcc actccgagcg ctacgaaccc aaatactaac ccccctatcc gtcctatccg
     1501 tcctaagtgt cctataggtc ccataggtcc tatcccctcc tatccgtcct attttccttt
     1561 ttcccccact tactgtataa tgttgagctt ttgcacactt accgcaacgg aggacgcagt
     1621 gataacggta ttcatagatg acaaggagtg ttccgcgagg gagggcagca caatcatcga
     1681 cgcggcccgg gagaacggaa tagacatacc cagcctcggg tacgatcccc gcgtaagccc
     1741 accgaccaac attgagatat ccatggtgga ggtggtggag aacggaaggc cccgcaccgt
     1801 ctcggcgaca tccaccgtca tggaggacgg gatggtcatc accacccgct cgcccgccct
     1861 cgaaagcttc aggaagatat accttcaggc cctcctgagg aaccactacg gggactgcgt
     1921 cgctccctg
//
LOCUS       DAOQTR010000454         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_76886_length_1927_cov_2.478632, whole genome shotgun sequence.
ACCESSION   DAOQTR010000454 DAOQTR010000000
VERSION     DAOQTR010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76886_length_1927_cov_2.478632"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKN85_09805"
     CDS             <1..425
                     /locus_tag="PKN85_09805"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT44748.1"
                     /translation="TGCPVSGDAEKVFRIIEEAGGVIVALDACSGFKPFMTDIEEGTG
                     DPVRALSERYLKIPCSCMTPNRRRLTEMTRLIEKFRPDAVIDVVLQACHSFNIESRKV
                     GQHVEKVHGLPFLKIVTDFSLGDAEQIRTRVEALLESC"
     gene            508..1620
                     /locus_tag="PKN85_09810"
     CDS             508..1620
                     /locus_tag="PKN85_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT44749.1"
                     /translation="MHLFKSLKLMSFVAIFAVFVLVSGALAADVPNLKVGYIFTSHHT
                     SFIVAAKKGEVFKDIGVYLRPVVDKEKYELVANGKPIAVFQLIVAKSGSETAALFARN
                     QLDLAMASVTAIMAGIDKGTPMKILSPLQTEGMGLVVPKDSPLTDWKSFMEYVTKAVK
                     PVKIGYHSPTSAPKIVLEAALKNAGIKVTEDPNDQSAKVLLADLKGTTNMIPALASKQ
                     VDAIVGPSPFPEVAVSRGVGKILIDLKDLPPKGYWHDFPCCVTAASDQIIAKHQEVVQ
                     KFVELIAKANVWCNKNRMEAATITAEWIGLPADAAKASTLVFLAKFNKSWMRGADKVL
                     SVLNGMGNFTGSLKGKKIDDVKPVLFDTRFIDKVKM"
     gene            1622..>1927
                     /locus_tag="PKN85_09815"
     CDS             1622..>1927
                     /locus_tag="PKN85_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44750.1"
                     /translation="MGLKSMLVRAGAYLLVPCLFLVFWDIMARQLNNEVILPGVGQVT
                     ELFVRPTESLIAMGSLAINVAISLVRVLAGYLLAVCIAVPLGILMGYYGTAFRLLNGF
                     "
BASE COUNT          412 a          567 c          572 g          376 t
ORIGIN      
        1 taaccggttg tcccgtcagc ggagacgcgg aaaaggtctt caggatcatt gaagaggccg
       61 gaggcgtcat cgtcgccctc gacgcgtgct cgggattcaa gcctttcatg acggacatcg
      121 aagaaggtac cggagacccc gtaagggcgc tcagcgagcg ttatctcaag ataccctgct
      181 cctgcatgac ccccaacagg cggcggctga cagaaatgac ccgtctcata gagaagttca
      241 ggcccgatgc cgtcatcgac gtcgtgctcc aggcgtgcca ttccttcaac atcgaatccc
      301 gcaaggtggg tcagcacgtt gagaaggtcc acggcctgcc gttcctcaag atcgtgacgg
      361 acttttccct gggcgacgcc gagcagatac gcacccgcgt ggaggccctg ctcgagtcct
      421 gctgagccct gtcgcacgtt ggtatcgatc cggtaaaagg cagcgctttt tccggtacag
      481 aaacatcaca ataacgagga ggtatgaatg cacctgttca agagtctgaa gctgatgtcc
      541 tttgtcgcga tctttgcggt tttcgttctt gtctccggcg ccctggccgc cgacgtcccg
      601 aacctgaagg tgggatatat cttcacgtcc catcacacat cgttcatcgt ggcggcaaaa
      661 aaaggggagg tcttcaaaga cataggggtc taccttcggc ccgttgtcga caaggagaag
      721 tacgagctgg tagccaatgg taagcccata gccgtcttcc agctgatcgt ggcgaagagc
      781 ggatccgaaa cagccgccct cttcgcgcgc aatcagctcg atctggccat ggcctcggtg
      841 accgccatca tggccggtat cgacaagggc accccgatga agatcttaag cccgctgcag
      901 acagagggca tgggtctcgt ggtccccaag gattccccgc tcaccgattg gaaatctttc
      961 atggagtacg taacgaaggc tgtgaaaccc gtcaagatag ggtaccactc gcctacgagc
     1021 gccccgaaga tcgtcctgga agcggcgctg aagaatgccg gcatcaaggt cacggaagac
     1081 cccaacgacc agtcggccaa ggtccttctc gcggacctca agggcacgac gaacatgatc
     1141 cccgctctgg cgagcaagca ggtcgacgcg atagtggggc cctcaccctt ccctgaggtc
     1201 gccgtcagcc ggggcgtggg aaagatcctc atcgacctta aggacctgcc tcccaagggc
     1261 tactggcatg acttcccctg ctgcgtcacg gcggcgagcg accaaatcat cgccaaacac
     1321 caggaggtcg tgcagaagtt cgtggagctc atagcgaaag ccaacgtctg gtgcaacaag
     1381 aacaggatgg aggcggcaac catcaccgcc gagtggatcg gcctgcccgc tgacgcggcc
     1441 aaggcgtcga cactcgtctt tctggcgaag ttcaacaaga gctggatgcg gggcgccgac
     1501 aaggttctgt ccgtcctcaa cgggatggga aacttcacgg gctcgctgaa gggcaagaag
     1561 atcgacgatg tgaaaccggt cctcttcgac acgcgcttca tcgacaaggt gaagatgtag
     1621 aatgggcctg aagagcatgc ttgtcagggc gggagcctat ctgctcgtgc cctgcctctt
     1681 ccttgtgttc tgggacatta tggcccggca gctgaacaat gaggtcatcc tgcccggtgt
     1741 cgggcaggtg accgaattgt tcgtgaggcc gacggagagc ctcatcgcga tgggttccct
     1801 cgccatcaac gtcgccatca gcctcgtcag ggtgctggcg gggtatctgc tggctgtctg
     1861 tatcgcggta cctctgggga tcctgatggg atattacggc acggccttca ggctcctgaa
     1921 cggcttc
//
LOCUS       DAOQTR010000455         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_77367_length_1919_cov_3.150215, whole genome shotgun sequence.
ACCESSION   DAOQTR010000455 DAOQTR010000000
VERSION     DAOQTR010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77367_length_1919_cov_3.150215"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKN85_09820"
     CDS             <1..462
                     /locus_tag="PKN85_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44751.1"
                     /translation="VKDIGFMADSIRVPTNTESLIVLNCTFQTRAGADGKKEPVTTRM
                     LNEIYQKAADNDPENLVIFSTEQNVSTDLIGTDAAIVIEGQFNHTRTAFIDVDVAGIP
                     NVPEELLKAAPKGNIRVPVVHAKIFGWYDNEYGSYTNRMGDLAVYLHKSMA"
     gene            complement(612..>1919)
                     /locus_tag="PKN85_09825"
     CDS             complement(612..>1919)
                     /locus_tag="PKN85_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT44752.1"
                     /translation="VTGKKFGNPRNPLLLSVRSGATISLPGMMHSFLNVGLNETIAEG
                     LSRFREYRWAAWDSYRRFLQTWGMFQGLERNIFDEIMNSYKLKYSVSKKIQFEPDQMR
                     QLALSYKKAMEDRGIVISNDPHKQLQEAIMLVFGSWYSQQARIYRHQMHVSDEWGTAV
                     IVQTMVFGNLNENSGSGVIFTRDPRGSSQNVVLYGDFIFGVQGDDIVSGLVETYPISE
                     KQRVSEKRQSLISLEKRFPEIFGELVRVAEFLVYEKRFNHQEIEFTFETASPRGLYIL
                     QTRDMVQRGAGKVVSFKDSPELERSQLGIGIGVSGGALSGRVVYSEEEIERYRRENVK
                     EPLILIRPDTVPDDVGIILKVEGILTARGGGTSHAAVTIPQLNKVGVVGFSKLHVYET
                     EAYSTCDGNVIRGGDYISIDGWTGAVYLGKHEIEKEESYKITL"
BASE COUNT          405 a          573 c          535 g          406 t
ORIGIN      
        1 gtgaaggaca tcgggttcat ggccgattcg atccgcgtcc cgacgaacac ggagtctttg
       61 atcgttctca actgcacctt ccagacccgg gcgggggcgg atggcaagaa ggagcccgtg
      121 accaccagga tgctcaacga gatatatcag aaggccgcgg acaacgaccc cgaaaacctg
      181 gtcatcttct caacggagca gaacgtctcc acggacctca tcggcacgga cgccgccatc
      241 gtcatcgagg gccagttcaa ccacacgagg accgccttca tcgacgtcga cgtcgccggc
      301 atcccgaacg taccggagga actcctcaag gcagcgccca aggggaacat ccgggtaccc
      361 gtcgtccacg cgaagatctt cggatggtac gacaacgaat atggcagtta caccaacaga
      421 atgggcgacc tggccgttta cttacacaaa agcatggctt agaaacagtt ttaaatttta
      481 ggttttaagt tttaagcaga acaacaaaaa caaagacata aagagcggct ccggagggta
      541 acaaccgggg ccgctctttt cttcccgtcc ttcacctaaa acttaaaacc taaaacttaa
      601 aaccgttctt ttcacagcgt tatcttgtac gactcctctt tttctatctc atgcttgccg
      661 aggtagacgg ctcccgtcca gccgtctatg ctgatgtagt ccccgcccct gatgacgttg
      721 ccgtcgcagg tgctgtaggc ctctgtctcg tagacgtgga gctttgagaa accgacgacc
      781 cctaccttgt tgagctgggg gatggtgacg gcggcatgcg aggtgcctcc cccgcgggcc
      841 gtgaggatgc cttcgacctt gaggatgata cccacgtcat cgggcacggt gtcgggcctg
      901 atgaggatga ggggctcctt cacattttca cgccggtagc gttctatctc ctcctcggaa
      961 tagacgacgc ggccggagag ggctcctccg ctcaccccga tccctatccc gagctgcgac
     1021 ctctcgagtt ccgggctgtc cttgaatgaa acgaccttgc ccgcgccccg ctggaccatg
     1081 tcccgtgtct ggaggatgta aagacccctc gggcttgccg tctcgaaggt gaactcgatc
     1141 tcctggtgat tgaaccgttt ctcatagacc aggaactcgg caacgcgcac cagttccccg
     1201 aatatctcgg gaaaacgctt ctccagggat atgagcgact gtctcttctc ggatacccgc
     1261 tgcttctccg atatgggata ggtctcgacg aggccagaaa caatatcatc gccctggacg
     1321 ccgaatatga agtcaccgta gaggaccacg ttctgcgagg acccccgggg gtcgcgggta
     1381 aagatgacgc cggacccgga gttctcgttg aggttcccga agaccatcgt ctgcacgatg
     1441 acggccgtgc cccattcgtc ggacacgtgc atctggtggc ggtagatgcg cgcctgctgc
     1501 gagtaccagg agccgaaaac gagcatgatc gcctcctgga gctgtttgtg agggtcgttg
     1561 ctgatgacga tgcccctgtc ctccatcgcc ttcttgtagg aaagggcaag ctgtctcatc
     1621 tggtcgggct cgaactgtat cttcttggag acgctgtact tcagcttgta gctgttcatg
     1681 atctcgtcga aaatgttcct ctcgagaccc tggaacatgc cccacgtctg gaggaaccgc
     1741 cggtaggagt cccaggcggc ccagcggtat tccctgaacc tgctgagacc ctcggctatc
     1801 gtctcgttga gccccacgtt caggaaggag tgcatcatgc cgggcaggga gatcgtggcg
     1861 ccgctccgga cggacaggag cagcgggttg cgcggattgc cgaacttctt ccccgttac
//
LOCUS       DAOQTR010000456         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_77640_length_1915_cov_3.073118, whole genome shotgun sequence.
ACCESSION   DAOQTR010000456 DAOQTR010000000
VERSION     DAOQTR010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77640_length_1915_cov_3.073118"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKN85_09830"
     CDS             <1..1039
                     /locus_tag="PKN85_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNT44753.1"
                     /translation="TPDGRYLVLPSEDGVAVHDLSTGRHEMSFPLAERVEGPLALYVA
                     GDGQRLVAVSGHSGFLGSYNLGTGTPIAGFPLVGMEPFQAFCMGGQVLATASRTVLSF
                     RDERTGRLLKRCDHNDYIDDILFVPSRERVIAAGLRGFLFWDREGSEPVMEKPDNADG
                     VITFGGCPSLSPGETNIIVRGRYYIALWDIETHRFLWRHDFPRENRIRKVVAFPDGER
                     FATLDITGVITVRSVDKGDVLATLHVLPEGFIWETPPDDHAPSGWLWTDREDLITVTA
                     ASGNDGGSRIFREGESEHRAYLRIYNNREMVTAKIAGKEEYRQQVALHARVLDRARID
                     NGSVGLRPALP"
     gene            1039..1233
                     /locus_tag="PKN85_09835"
     CDS             1039..1233
                     /locus_tag="PKN85_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44754.1"
                     /translation="MGIIDTGTDPGEHNIISNASRAAILPAHIVKAPLLPSLPIIGHR
                     PLPIITGRMRGFDLSCHPLF"
     gene            1346..1633
                     /locus_tag="PKN85_09840"
     CDS             1346..1633
                     /locus_tag="PKN85_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44755.1"
                     /translation="MTRDEEIRYGMEAYRDALRFAAGASTREDLAGVEESYWIAFNKL
                     RESPLEEHRRICAECLEAVARILDGEGRRDAGNDLREQAETFRKPRAGEKR"
     gene            1655..>1915
                     /locus_tag="PKN85_09845"
     CDS             1655..>1915
                     /locus_tag="PKN85_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44756.1"
                     /translation="MGHHSLIAWIREEIMSTEVVMRSEVVGYTERLEPNPAMSISEGT
                     LYPTTSDGMYEIHQDPIYEDVPRTVLDERRASDAAEALVPLIL"
BASE COUNT          396 a          584 c          582 g          353 t
ORIGIN      
        1 gacacccgac gggaggtatc tggtactgcc gtcggaggac ggtgtcgcgg tccacgacct
       61 gtcgaccggc cggcatgaaa tgagcttccc tctcgccgaa cgggtggagg gtcctttagc
      121 cctctacgtg gccggggacg ggcaaaggct ggtggcggtc tcaggacact cgggctttct
      181 cgggtcctac aaccttggaa cgggcacccc tattgccggt ttccccctcg tcggcatgga
      241 gccgttccag gcattctgca tgggtggaca ggtcctcgcc accgcgagcc gaacggtcct
      301 ttcattccgt gatgaacgaa cgggcaggct cctgaagaga tgcgaccaca acgactacat
      361 cgacgacatc ctcttcgtcc cgtcccggga aagggtgatc gctgcgggat tgagagggtt
      421 cctcttctgg gaccgggaag ggagcgaacc tgtgatggag aaaccggaca atgcggacgg
      481 cgtcatcaca ttcggcggat gcccgtcctt atcgcccggc gaaacgaata tcatcgttcg
      541 gggccggtac tacatagccc tgtgggacat tgagacacac cggttcctgt ggcggcacga
      601 ctttccccgg gagaaccgga taagaaaggt cgtcgccttt cccgacggcg aacgcttcgc
      661 gacactggac ataacggggg tcataacggt ccgtagcgtc gataaagggg atgtcctcgc
      721 gaccctccat gtcctgcccg aagggttcat ctgggagacc ccgcccgatg accatgcacc
      781 ttcggggtgg ctctggacgg acagggaaga ccttatcacc gtcacggccg cgtcggggaa
      841 tgatggcggc tcaaggatat tccgagaggg tgaaagcgag cacagggcct acctgaggat
      901 atacaacaac cgggaaatgg tcacggcgaa gatcgcggga aaggaagagt accgccagca
      961 ggtcgcgctc cacgcccgcg tcctcgacag ggcacggatc gacaacggct ccgtcggtct
     1021 tcgccccgcc ctgccctgat gggcatcatc gacaccggaa ccgaccccgg ggaacacaac
     1081 atcatctcca acgcctcccg cgccgccatt ctccctgccc acatcgtcaa ggctcccctc
     1141 ctcccgtccc ttcccattat cgggcaccgg cccctgccga tcatcacggg aaggatgaga
     1201 ggcttcgatc tctcctgcca tccgcttttt tagcccccaa tccccacttt gtgcatgata
     1261 caccttctct tttgccattg cgtgcacggg actttgcctg atatccaatt aaaaaacgct
     1321 ctatgatccg tcaatggagg tgaccatgac aagagacgag gaaataaggt acgggatgga
     1381 ggcatacagg gatgccctgc ggttcgcggc cggggcttcc acgcgggaag accttgccgg
     1441 tgtggaggag agctactgga tcgcctttaa caaactgaga gagtctcccc tcgaggagca
     1501 ccggcgtatc tgcgccgagt gtcttgaagc ggtggcgcga attctcgatg gggagggtcg
     1561 acgggacgcc ggcaacgatc ttcgggagca ggccgagacc ttccgcaagc cccgcgccgg
     1621 agagaaacga taacgcaaga aggggtcggg gaacatgggg catcacagtc tcatcgcctg
     1681 gatacgggaa gagatcatgt ctacggaggt ggtcatgcgg agtgaggttg ttggctatac
     1741 agagagattg gagcccaatc ccgccatgtc tatctccgag ggcactcttt atcctacaac
     1801 ctcggacggg atgtacgaga tccaccagga ccctatctac gaggatgtcc cccggaccgt
     1861 cctcgacgag aggcgcgcgt cggatgccgc cgaggcgctc gtccccctca tactc
//
LOCUS       DAOQTR010000457         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_77794_length_1913_cov_3.200753, whole genome shotgun sequence.
ACCESSION   DAOQTR010000457 DAOQTR010000000
VERSION     DAOQTR010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77794_length_1913_cov_3.200753"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..443
                     /locus_tag="PKN85_09850"
     CDS             72..443
                     /locus_tag="PKN85_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44757.1"
                     /translation="MGGITVHREGMMNVRELAEVLGLEVLTGETNMDRPVRSGYTSDL
                     LSDVIANIGDDAVWITIQRHVNILGVAKLKDVSAIIIPRKLDLEGSFLEKAKQEGIAV
                     LRGTSSAFELSALVYERLKRG"
     gene            451..1200
                     /locus_tag="PKN85_09855"
     CDS             451..1200
                     /locus_tag="PKN85_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT44758.1"
                     /translation="MPEFACDLHIHSTLSPCGSLDMSPRAIVEKAREAGLDIIAVTDH
                     NMAENTPYVREAGERSGLVVLAGMELQTMEEVHVIAIFDDYDTAYALQLMVYDLLPPV
                     ANDVEFWGDQVVVDGEDNIVRSEERLLISSAGISIEEAVSWIKSHGGIAIASHVDSPT
                     FSIISQLGFVPADIPFDALEIRDRGKAGDLMPFIMRKGLPFVTFSDAHYPGDIGKRRT
                     HLTLDAPDCVSIEGALRALGEQSLTGQAGRS"
     gene            1220..1750
                     /locus_tag="PKN85_09860"
     CDS             1220..1750
                     /locus_tag="PKN85_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44759.1"
                     /translation="MMEDIASHIMDIAMNSVTAGAKRIGIAVEKDPRTAMLSLTVADD
                     GAGMDPETVLKVQDPFFSTKTGRKVGLGIPLLKGTAETTDGSFELTSAKGAGTRIRVT
                     FDCRHPDLPPLGNIRDTFLVLIVSHPDVDFDLRYSVDGRTLELDTEEMKKELGDVPVN
                     HPEVIGFLGKYLDEHL"
BASE COUNT          440 a          487 c          638 g          348 t
ORIGIN      
        1 gggcgaccca ggacaggggc actgacgagt gccgggacgg tcagggggat gctggaggta
       61 ccatttaata tatgggcggg ataaccgtcc acagggaggg gatgatgaac gttcgtgagc
      121 ttgcggaggt gctgggactt gaggtgctaa cgggagagac gaacatggat aggccggtga
      181 ggtcggggta cacctccgat ctgctctcgg atgtgatcgc caacatcggt gacgatgcgg
      241 tgtggatcac gatccagcgg catgtgaaca ttcttggagt ggcaaagctc aaggacgtgt
      301 ccgccatcat catcccgcgg aaactggacc tggaaggatc cttcctggag aaggcgaaac
      361 aggagggtat agcggtcctc aggggaactt cttcggcatt cgagctctcc gctctcgtgt
      421 acgaacgtct gaagagaggt tagagaggac ttgccggaat ttgcctgcga cctgcatata
      481 cactcgaccc tgtcaccctg cggcagtctt gacatgtcgc cccgggcgat cgtcgagaag
      541 gcgcgggagg cgggactgga tatcatagcc gttacggacc ataacatggc ggagaacaca
      601 ccatatgtca gggaggcggg agagcgcagc gggctcgtcg tccttgcggg catggagctt
      661 cagaccatgg aggaggtcca tgtcatcgcc atcttcgacg actatgacac cgcgtacgcg
      721 ctgcagctca tggtctacga tcttctgccg ccggtggcca acgacgtgga gttctggggg
      781 gaccaggtgg tcgtggacgg ggaggacaac atagtccgca gcgaggagag gctgcttatc
      841 agctcggccg ggatatccat agaggaggcc gtgtcctgga taaaatccca cggggggatc
      901 gccatcgcct cccatgtcga cagcccgacc ttcagcatca taagccagct tggtttcgtg
      961 ccggcggaca ttccctttga cgccctggag atacgcgacc ggggcaaggc cggggacctc
     1021 atgcccttta tcatgaggaa ggggctgccc tttgtgacat tctccgatgc ccactatccg
     1081 ggggacatcg gaaagaggag aacacatctg acgctcgacg cgcccgattg cgtgagcatc
     1141 gaaggggccc tcagggccct gggagaacaa tcgctgaccg ggcaggcggg gcggtcgtga
     1201 agagacggga atcgtcatca tgatggagga tattgcatca catatcatgg acatagcgat
     1261 gaactcagtg acggcggggg cgaaacgcat cggtatcgcg gtggaaaagg acccgcggac
     1321 ggcgatgctg agcctcacgg tagccgatga cggggccggg atggaccctg aaacggtgtt
     1381 gaaggtgcag gaccccttct tctccacgaa gacgggaaga aaggtggggc tcgggatacc
     1441 tctcctcaag ggaacggcgg agacgacgga cggcagcttc gagctgacga gcgcgaaggg
     1501 cgcggggaca aggataaggg tcaccttcga ctgccggcac cccgatctgc ccccgctggg
     1561 gaacatacgc gacacctttc tcgtactcat cgtgagccac cccgatgtcg atttcgatct
     1621 tcgctattcc gtcgacggca ggaccctgga actcgatacg gaagagatga aaaaggaact
     1681 cggtgacgtt ccggtgaacc atccggaagt cattggtttc ctaggcaaat atcttgacga
     1741 acatctatga ttttatatag attgttatca tcataaggag gccgagagat gaatcttgaa
     1801 gagctgaaaa aaataaggga aaaggcacaa aaagatgtgg agctgcggca gaagcaggca
     1861 cgggtgcgga tcgtcgtcgg gatggggacg agcggaatag ccgccggcgc gcg
//
LOCUS       DAOQTR010000458         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_77962_length_1911_cov_2.543103, whole genome shotgun sequence.
ACCESSION   DAOQTR010000458 DAOQTR010000000
VERSION     DAOQTR010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77962_length_1911_cov_2.543103"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="PKN85_09865"
     CDS             complement(<1..1899)
                     /locus_tag="PKN85_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT44760.1"
                     /translation="MKTPVAFIGGVAANAGMRTALKEVFELTEDTFFVPGNYTSIGAA
                     GAVYAVLDDPSLTVSFTGLDRLNAYLNEERQEATHEPLFLSAANMNVAYDMKPVTRGI
                     KVYLGVDVGSISTNLVLIDEERNVLAKRYLMTEGRPLEAVKRGLAEIGEEVGDMVEII
                     GAGTTGSGRYLTADFIGADIVRNEITAQAQAAIAIDPGVDTIFEIGGQDSKYISVDNG
                     VIVDFEMNKACAAGTGSFLEEQAERLDISIKEEFGVLALRSKKPVKMGERCTVFIESD
                     VIHQQQRGADREDIVSGLAYSIVQNYLNKVVVDRRVGKRIFFQGGTAFNKGVVAAFEK
                     VLGMPITVPSHHDVTGAIGVAILAMKEKAWEKSGFKGFDLSKRSYTVDTFECKGCENL
                     CEIRKVTVEKETPLYYGSRCEKYDVVRRGEKKEMADLFSMREEILNEIYDRKAGKPTI
                     GIPKVLHMHELLPFWKSFLTELGFDVVVSDVTNKKTVRDGVENIIVESCFPIKLAHGH
                     VMNLLQKGIGNIFIPSVISLRKPSKHARNSFACPYAQSLPYTIKGSIDFDDRQARVLT
                     PIIRFGEGDEAVLANLVEHFKPFGKSRRSVKKAFETALRVQDSFYRRLTEMGRAFLDE
                     MRPDDKVMV"
BASE COUNT          340 a          602 c          541 g          428 t
ORIGIN      
        1 aaccatcacc ttgtcatcgg gacgcatctc gtcgagaaag gccctgccca tttccgtgag
       61 gcgccgatag aaggaatcct ggacgcgaag tgccgtctcg aaggccttct tcacgctcct
      121 tctcgacttg ccgaagggct tgaagtgctc cacgagattg gcgagaacgg cctcgtcacc
      181 ctccccgaag cggatgatgg gcgtcaggac ccgggcctgc ctgtcatcga aatcgatgga
      241 ccccttgatc gtgtagggca aagactgcgc gtagggacag gcgaaggagt tccgtgcatg
      301 cttcgacggc ttcctgaggc ttatgacgct ggggatgaag atgttgccga tccccttctg
      361 gaggaggttc atcacgtggc catgggcgag cttgatgggg aaacagctct ccacgatgat
      421 gttctccacg ccgtccctca cggtcttctt gttggtcacg tcggagacga ccacatcgaa
      481 gcccagttcc gtcaggaaac tcttccagaa cggcaggagc tcgtgcatgt ggagcacctt
      541 cggtatgcct atcgtgggct taccggcctt cctgtcgtag atctcgttga ggatctcctc
      601 acgcatggaa aagagatccg ccatctcctt cttttcaccc ctgcgcacca catcgtactt
      661 ctcgcatctg ctgccgtaat acaggggggt ttctttctct acggtgacct tgcggatctc
      721 gcagaggttc tcgcacccct tgcattcgaa ggtgtcgacg gtgtaagacc tcttgctcag
      781 gtcaaaacct ttgaaaccgc tcttctccca cgccttctcc ttcattgcca ggatggcgac
      841 gccgatggcg cccgtcacgt cgtggtggga aggtaccgtt atcggcatgc ccagcacctt
      901 ttcgaaggcc gcgacgacgc ccttgttgaa ggccgtgccg ccctggaaga atatccgctt
      961 gcccaccctt ctgtcgacga cgaccttgtt gaggtagttc tgcacgatgg aataggcaag
     1021 tcccgagacg atgtcttccc tgtcggcgcc ccgctgctgc tggtggatga cgtcggactc
     1081 gatgaaaacg gtgcaccgct cgcccatctt caccggcttc ttcgacctga gggcgaggac
     1141 gccgaactcc tcctttatcg aaatgtcgag cctctcagcc tgctcttcca ggaaggagcc
     1201 cgtgcccgcc gcgcaggcct tgttcatctc gaagtcgact atcacaccgt tgtccacgct
     1261 gatatacttc gagtcctgac cgcctatctc gaagatggtg tccactccgg ggtcgatggc
     1321 tatggccgcc tgggcctggg cggtgatctc gttgcggacg atgtcggcac cgatgaagtc
     1381 cgccgtcagg taacggcccg acccggtggt gccggcgccg atgatctcca ccatatcgcc
     1441 gacctcttcc cctatctcgg caaggcctct cttgaccgcc tcaagcggcc ggccttccgt
     1501 catgaggtac ctcttggcga ggacattcct ttcctcgtcg atgaggacga ggttcgtgct
     1561 tatggacccc acgtccacgc ccaggtagac ctttatcccg cgcgtcacgg gcttcatgtc
     1621 gtaggccacg ttcatgttcg cggccgagag gaagagcggc tcgtgagtgg cctcctgtct
     1681 ttcctcgttg aggtaggcat tgagcctgtc aagcccggta aaggacaccg tgagcgatgg
     1741 atcatcgagc accgcgtaga cggcacccgc ggcgccgatg gaggtatagt tcccgggaac
     1801 gaagaaggtg tcctccgtca gttcgaagac ctctttcaag gcggtcctca tcccggcgtt
     1861 cgccgccacg ccgccgatga acgcgacagg cgtcttcacg tccttgccct t
//
LOCUS       DAOQTR010000459         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_77965_length_1911_cov_2.516703, whole genome shotgun sequence.
ACCESSION   DAOQTR010000459 DAOQTR010000000
VERSION     DAOQTR010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77965_length_1911_cov_2.516703"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1911)
                     /locus_tag="PKN85_09870"
     CDS             complement(<1..>1911)
                     /locus_tag="PKN85_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT44761.1"
                     /translation="FLKGYFDHGIVLYSRTGRVIGQVPHAPELSGKDFSDREYFQYTM
                     KTKAPYISKPFRSIRPPYDPIVMFSVPVFHKDGEIAAVLGGAVSLLKDVTFGGIAGIK
                     IGRTGYLYLFNTDRTIIVHPDKTRILKQDLPRGANHLFDAAIDNWFEGSGETAPSKDV
                     PGIATFKRFRNTDWILAAHYPIKDAYAPIYRERWYIVAYTAIGIGLCVLIILIVMRRN
                     LSPLSELASQAEGIGRTGDPLGPVHVDTGGEIGALASSFNAMLERLAEREASLKETME
                     ELRKLHNAVEHSSAVVVITDGKGNIEYVNPKFVEVTGYERSEVSGKTPRILKSGELPP
                     EIYDDLWSTITSGEEWQGVFHNRKKNGELYWAAASISPLKDATGTITHFVGVQEDITA
                     VKLFEQELKGAKEAAETANQAKSDFLASMSHEIRTPMNAIIGMAELLLEGPLDEEQRR
                     YVTTLRNAGDNLLNIINDILDISKIEAGYLELHETAFNVRELVDAVCDTMAIRVREKG
                     LTLDCIIGADVPGHLSGDVGRLRQVLLNLVGNAVKFTEEGGITITVERDGASGGNDVL
                     IFSINDTGIGIPQDKIPHIFEKFTQADSSTTRKYGGTGLGLTISRRLVELMGGRIWVT
                     STVGAGSTFFFTA"
BASE COUNT          336 a          556 c          577 g          442 t
ORIGIN      
        1 gccgtgaaaa agaaggtgct tcccgcgccg acggtgctcg ttacccatat gcggccgccc
       61 atgagctcca cgagacgccg ggatatggtg agccccagcc ccgtgccgcc gtactttcgc
      121 gttgtcgacg aatcggcctg agtgaacttc tcgaatatgt gagggatctt atcctgtggt
      181 atgccgatgc ccgtgtcgtt gatggagaag atgagaacgt cgttcccgcc gctcgccccg
      241 tcacgttcga cggtgatcgt tattccgccc tcctcggtga acttcacggc gttgccgacg
      301 agattgagaa ggacctgcct caaacgtccc acgtcgcccg aaagatgccc cggcacgtcg
      361 gctcctatta tgcagtcgag ggtgaggcct ttttcgcgta cgcgtatcgc catggtgtca
      421 cagacggcat cgacgagttc cctgacgttg aaggcggtct cgtgaagctc caggtagccc
      481 gcctcgatct tcgatatgtc caggatgtcg ttgatgatgt tgaggaggtt gtccccggcg
      541 ttccgcagcg ttgtgacata ccttctctgc tcctcgtcga ggggcccctc cagaagcagc
      601 tcggccatgc cgatgatggc gttcatcggc gtgcgtatct cgtggctcat gctggcgagg
      661 aaatcgctct tggcctggtt ggcggtctcg gccgcctctt tggctccctt gagctcctgc
      721 tcgaagagct tcacggcggt gatgtcctcc tggaccccga cgaaatgcgt aatggtgcct
      781 gtcgcgtcct tgaggggaga gatggatgcc gcggcccagt agagctcccc gttcttcttc
      841 ctgttgtgaa agacgccctg ccattcctca ccggaggtga tggtcgacca gagatcatca
      901 tagatctcgg gcggcagctc tcccgatttg aggatacgcg gcgtcttgcc tgaaacctcg
      961 cttctttcat aaccggtaac ctcgacgaac ttgggattga cgtattcgat gttgcccttc
     1021 ccgtcggtga tgacgacaac ggccgaactg tgttcgacgg cattgtggag cttgcggagc
     1081 tcttccatgg tctccttcag agacgcttcc ctttcggcga gacgttcgag catggcattg
     1141 aaggaagaag ccagcgcccc tatctcccca ccggtgtcca cgtgcacggg gccaagggga
     1201 tctcccgtcc ttcctatccc ttcggcctgc gaggccagct ccgaaagggg cgacaggttc
     1261 ctgcgcatca cgatgaggat gatgagcacg cacagtccga tgccgatggc cgtgtaggcc
     1321 acgatgtacc accgctccct gtagatgggc gcataggcgt ccttgatcgg ataatgtgcc
     1381 gccagtatcc agtcggtgtt ccgaaagcgc ttgaaggtcg ctatgcccgg cacgtctttt
     1441 gagggcgccg tttccccgct tccctcgaac cagttgtcga tggcggcgtc gaaaaggtgg
     1501 ttcgctcccc ggggtaggtc ctgtttaagt atccgtgtct tgtcgggatg gacgatgatc
     1561 gtgcggtccg tgttgaagag gtagaggtaa cccgtccggc ctatcttgat gccggctatt
     1621 cccccgaagg tgacgtcttt gagaagggac acggctccgc ccaggacggc ggcgatctcc
     1681 ccgtccttat ggaagacggg gacggagaac atgactattg ggtcgtaggg cggccttatc
     1741 gacctgaagg gcttcgagat gtatggcgcc ttcgtcttca tcgtgtactg gaaatattcc
     1801 ctgtcggaga agtcttttcc ggacagttcg ggggcatggg gcacctggcc gatgacccgt
     1861 cccgtccttg aatagaggac gatgccatgg tcgaaatatc ccttcagaaa g
//
LOCUS       DAOQTR010000460         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_78431_length_1904_cov_3.083288, whole genome shotgun sequence.
ACCESSION   DAOQTR010000460 DAOQTR010000000
VERSION     DAOQTR010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78431_length_1904_cov_3.083288"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKN85_09875"
     CDS             <1..463
                     /locus_tag="PKN85_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44762.1"
                     /translation="GQVHAGAALAVTEEEVIGIRLKGIEDIPRPSPNAVAILVLIKLA
                     AISGDDMYRDHAAKALEAFSSEASPMAIHGAYCLCALEAFYRMVKLELRAGAVSELAE
                     ASLFSYHPYTCITWGDPGDNIIIPCIATTCYEPLTSAEALKEFLEAREPGI"
     gene            586..1203
                     /locus_tag="PKN85_09880"
     CDS             586..1203
                     /locus_tag="PKN85_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNT44763.1"
                     /translation="MSVAKHLKKKFITGLFVLIPLIVTAYIIYVVVSFIEGFIGPALK
                     NIFFQVFQREVYVTGTACIIFVVITYLTGLFVSNYFGKTLFSRGERLVKRIPIVKSIY
                     GSVKDMMEAFSSEKIKSFKEVVLIEFPFQGRYAIGLVTNRVEIGGIKHCAVFVPTTPN
                     PTSGYIIITKEEELLFLDMATDDALKYIISLGTSRSGPIWNGKRS"
     gene            1182..1892
                     /locus_tag="PKN85_09885"
     CDS             1182..1892
                     /locus_tag="PKN85_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNT44764.1"
                     /translation="MEREKIIGALTGVRRVFLKSLAFIAVAGIVSFVYSKQMLYLLVK
                     ATGIKVYYLTIQEVFLATVQLALYAGIFFALPVIIFLAWHEMKDLYRTKRVFGYLLVF
                     AAIVLFYGGSFFCWKIVLPSGIKFLVTGYESDALMAMISVERYLTFSTAMIFAFGTTF
                     EVPLILLALGKLGIVKARMLVRTRRYALLGIVVASALITPTPDVYNLSLLAVPMYIFY
                     EIGIILVKMVEKQKDKDS"
BASE COUNT          403 a          571 c          481 g          449 t
ORIGIN      
        1 aggtcaggtc catgccgggg ccgccctcgc ggtcaccgaa gaagaggtga tagggatccg
       61 cctcaagggc atcgaggaca taccgagacc atcacctaac gccgttgcca tccttgtgct
      121 catcaagctc gcggcgatat caggcgacga catgtaccgc gaccacgctg cgaaggccct
      181 cgaagccttt tcgtccgagg catcccctat ggcgatccat ggagcctatt gtctttgcgc
      241 cctcgaggcc ttctaccgga tggtaaagct cgagctccgc gccggggctg tgtcggaact
      301 tgccgaagcc tccctcttct cttatcaccc ttatacgtgc atcacctggg gcgaccccgg
      361 cgataacatc atcatcccct gcatcgccac cacctgctac gaacccctca cctccgccga
      421 ggcattgaaa gagttcctgg aggccaggga acccggcata taaagcaagg taaagtattg
      481 tcttttttct tgtcttgaaa cggctcaagc gattcaaacg gctcaagcga ttcaaatggt
      541 cttttcattg actttcctcc cttccgacga tagtatgtcg acaccatgag tgtggcaaag
      601 cacctgaaaa aaaagttcat taccgggctt ttcgtcctca ttccgctcat agtcaccgcc
      661 tatatcatct acgttgtcgt ttccttcatc gaaggcttca tcgggcctgc cctgaagaac
      721 atctttttcc aggtctttca acgcgaggtc tacgtaacgg gcacagcctg catcatcttc
      781 gtcgtcatta cgtacctcac cggtcttttc gtctccaact acttcgggaa gacccttttc
      841 tcacggggag aaagactggt gaagaggatc cccatcgtga agagcatcta cggctccgtc
      901 aaggacatga tggaggcctt ctcgtcggag aagataaagt cgttcaagga ggtcgtcctc
      961 atcgaattcc cttttcaggg gaggtacgcg atagggcttg tgacgaacag ggtggagatc
     1021 ggggggatca agcattgcgc ggttttcgtg cccaccacgc cgaaccccac atcggggtac
     1081 atcatcatca cgaaggagga agaactcctc tttctcgaca tggcaactga cgacgcgctg
     1141 aagtacatta tctcgctggg tacatcccgg agcggaccca tatggaacgg gaaaagatca
     1201 taggcgccct gacgggggtg cgaagggtat ttctcaagtc actggccttc atcgccgtcg
     1261 ccgggatcgt cagcttcgtc tactcgaagc agatgctcta ccttctcgtc aaggcgacgg
     1321 gcatcaaggt ctattacctc accatccagg aggtcttcct cgccaccgtg cagcttgccc
     1381 tctacgcagg catttttttc gctctgcccg tcatcatatt tctcgcctgg cacgagatga
     1441 aggacctgta caggaccaag cgcgtctttg gctacctcct ggtgtttgcc gcgatagtcc
     1501 tcttttacgg gggcagtttc ttctgctgga aaatagtgct tccctcgggg atcaagttcc
     1561 tcgtcacggg ttacgaaagc gacgccctga tggccatgat atcggtggaa cgatacctca
     1621 ccttctccac tgccatgatc ttcgccttcg ggaccacctt cgaagtgccc cttatcctcc
     1681 tcgccctggg aaagctcggg atcgtaaagg cgaggatgct ggtgcgcacg cggcgatacg
     1741 ccctcctggg cattgtcgtc gcctcggccc tcatcacgcc cacgcccgat gtatacaacc
     1801 tctcgcttct cgcggtaccc atgtatattt tctatgagat cggcataatc ctcgtgaaga
     1861 tggtcgagaa acagaaggat aaggactcat gagtccgcgc atcc
//
LOCUS       DAOQTR010000461         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_78764_length_1899_cov_3.023319, whole genome shotgun sequence.
ACCESSION   DAOQTR010000461 DAOQTR010000000
VERSION     DAOQTR010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78764_length_1899_cov_3.023319"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1713)
                     /locus_tag="PKN85_09890"
     CDS             complement(67..1713)
                     /locus_tag="PKN85_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT44765.1"
                     /translation="MSTPTYPKHKKKDETTYRAHGWWTGVTLGDVIDKTADVFPDKEG
                     LVDDRSRLTFAEVRANVDRLALGLLDLGIKKGDAVLLQLPNWAEYVYSYFAMQKIGAI
                     PVILISGYKQLEVGHLANLTQAKAWIVPERYRKIDYTSFMGDVRKANPQLKNIIFVRS
                     ENKAGGDAINFESLLDRDVTAKDRNRLELAKPLPTDVCHVVPSGGTTGLPKGIPRTHN
                     DYICNVEYLHKGWEMNTTDACLVIVPVGHNLAVLNVVGSLLFAYKLVLSDSTRPADIC
                     KLIEDEKITYMPSVPSLVRRIMEAPEMGNFDLTSLRKISAGGEPSTPDLIQEVYRKLH
                     CTYINEFGMTEGLLCRSQLTDDIETICSTVGRATCPYDEVRIIDREGKKLPAGVDGEL
                     AAKGPGIFAGYLLAAGAKDQSFTKDGFFRTGDQAQMDAQGYIKITGRIKDIIIRGGEN
                     ISPAQVEEILCGHKEIADAAVIGMPDKDLGERVCAFVRLIPGAKLSEKDMVAYMEASG
                     ASKLLVPERFEFVDSFPMTQAGKHDKKLLKKELIERLGLK"
BASE COUNT          326 a          574 c          524 g          475 t
ORIGIN      
        1 atctgcccac atcccaatac gcccagtttc tttatttcca ttgtgatctc cttataacag
       61 cgttctttac ttcagaccca ggcgctctat gagctccttc ttgagaagct tcttgtcgtg
      121 tttaccggcc tgggtcatgg ggaagctgtc gacaaactcg aacctttccg gaacgaggag
      181 cttggacgca ccgcttgctt ccatgtaggc caccatgtcc ttctcggaca gttttgcccc
      241 cgggatgagg cgcacgaagg cgcagaccct ttcgccgagg tccttgtcgg gcattccgat
      301 gaccgcggcg tccgctatct ctttgtggcc gcagagtatc tcttcgacct gggcaggact
      361 gatattttcg ccgccgcgga ttattatgtc cttgatccgt cccgttatct tgatgtatcc
      421 ctgtgcgtcc atctgcgcct ggtcgccggt cctgaagaaa ccgtccttcg tgaagctctg
      481 gtccttcgcc ccggctgcca gcaggtaacc ggcgaatatc cccggccctt tcgcggcaag
      541 ttcaccgtca acaccggcgg gaagcttctt cccttcccgg tcgatgatcc tcacctcgtc
      601 gtaggggcag gtggcccgtc cgacggtgct gcaaatggtc tcaatgtcat cggtcagctg
      661 ggacctgcac aagaggccct ccgtcatacc gaactcgttg atgtacgtgc aatggagttt
      721 tctgtagacc tcctgaatga ggtccggggt actcggctcg cctccggcgg agatcttcct
      781 gagcgacgtc aggtcgaaat tgcccatttc cggggcttcc attattctcc tcacgaggct
      841 gggcacgctc ggcatatacg tgatcttctc gtcctcgatc agcttgcaga tatcagccgg
      901 tctcgtcgaa tccgacagga ccagtttgta cgcgaaaagc aatgacccga ccacgttgag
      961 gaccgccagg ttgtgcccga cgggaacgat gacgaggcac gcgtcggtgg tgttcatctc
     1021 ccagccttta tggaggtatt ccacgttgca tatgtagtcg ttatgtgtcc gcgggatacc
     1081 tttcggaagg ccggtggtgc cgcccgaggg aacgacgtgg cagacatcgg tcggcagggg
     1141 cttcgcgagc tccagcctgt tcctgtcctt cgcggtcacg tccctgtcga gaaggctctc
     1201 gaagtttatc gcgtcaccgc ctgccttgtt ctctgaacgg acgaatatga tgttcttgag
     1261 ctgggggttg gccttcctca cgtcccccat gaaggatgtg taatctatct tcctgtacct
     1321 ttccggcact atccatgcct tcgcctgggt caggttcgca agatgcccga cctcgagctg
     1381 cttgtagccg ctgatgagga tgacgggtat ggccccgatc ttctgcatgg caaaatagga
     1441 atagacgtac tcggcccagt tgggcagctg cagaagcacg gcatcgccct ttttgatccc
     1501 gagatcgaga aggccaaggg ccagccggtc gacattggcc ctaacctcgg caaaggtcaa
     1561 ccgcgacctg tcatcgacaa ggccttcctt gtcgggaaac acatcggccg tcttgtcgat
     1621 gacgtcgccg agcgtcacgc ccgtccacca gccatgagcc ctgtaagtgg tttcgtcttt
     1681 cttcttgtgt ttcggatatg tgggtgtgct catatctatg cccctctttg gactcgttcg
     1741 gaccggcctt attcctgttc ctgcttgatc gcgccgggtt ttgccatctt cggcaggaaa
     1801 ggctctggcg cgctggtata gccgtccggg tagcggacgc actttacctg ctcgtaccgc
     1861 gagtcggctt cgaggcacca gttgcaggcc gcgcacttc
//
LOCUS       DAOQTR010000462         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_79769_length_1884_cov_2.404593, whole genome shotgun sequence.
ACCESSION   DAOQTR010000462 DAOQTR010000000
VERSION     DAOQTR010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79769_length_1884_cov_2.404593"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..557)
                     /locus_tag="PKN85_09895"
     CDS             complement(81..557)
                     /locus_tag="PKN85_09895"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNT44766.1"
                     /translation="MTTNADSEVIELIESVLEAAKLNSMGYCCTQIMAIMMLRSMGRE
                     NPDLVRAVGGLCKGIVDAGETCGVLFGGVCLISLFLGKGSDDETAKGDLPFAIYDLAQ
                     WFERQTKGPYGGKKCVDILTVSPDKKACLRLIVGTYDKVLSILEAYGVRPGETARG"
     gene            complement(538..1227)
                     /locus_tag="PKN85_09900"
     CDS             complement(538..1227)
                     /locus_tag="PKN85_09900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT44767.1"
                     /translation="MENDERRACLQRMTGGTLRPGGLTLTTRALELCRMPPEARVLDA
                     GCGNGVTLRHLRERYGVRCCGLDLSMEMLAAARDEDGEGSLVRARMEEIPFRNGAFQG
                     VLCECVLSHTNAEVVVREFSRIVEEGGFLILSDLYHLFPGSAAPPGMEVLGRDWLEEC
                     LARSGFDIVTWEDRTADLRRLAAELIMSGCSLPPYHVDPGAAPAKAFHDWRGIGYYLL
                     VARRTNDNQCR"
     gene            complement(1236..1709)
                     /locus_tag="PKN85_09905"
     CDS             complement(1236..1709)
                     /locus_tag="PKN85_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44768.1"
                     /translation="MVKEGKRLLIDADVQAVLEKRGIREEDIQGVLDFGEETGNVYIH
                     PDTGHLLSYFTPATTTYWVEFGRDGGAYRIYRAYSHRMEILHGFNMPAKKQQTLDWTC
                     MKCDRRLELATVKLKYMEETFGVDIPACPSCQRIFVSEEDATQRMAQAEKMLEDK"
BASE COUNT          364 a          647 c          474 g          399 t
ORIGIN      
        1 caccgagatc tacactgtgt cagacactct ttccctacac gacgctctcc gacctcttga
       61 ccacccgtgc ggcacgggca tcacccacgc gcggtctccc cggggcgaac gccataggcc
      121 tcgagtatgg acagcacctt gtcgtaggtc cccacgatca gcctgagaca ggccttcttg
      181 tcgggactca cggtgaggat atcaacgcac ttctttccgc cgtagggtcc tttggtctgg
      241 cgctcgaacc actgcgcgag atcgtagatc gcgaagggaa ggtcgccctt cgccgtctcg
      301 tcgtcggaac ccttccccag gaacaaagag atgagacaga cacctccgaa cagaacgccg
      361 cacgtctccc cggcgtcgac tatgcccttg cacaacccgc cgacggcccg cacgaggtcc
      421 gggttctcac gacccatgct ccttaacatc atgatggcca ttatctgcgt gcagcaatag
      481 cccatgctgt tgagcttcgc cgcctccagg acactttcga tgagttcgat cacttcacta
      541 tcggcattgg ttgtcattgg tcctccttgc aacaagaaga tagtacccga tgccccgcca
      601 atcatggaat gcctttgcgg gagcagcccc ggggtccaca tggtacggcg gcaacgagca
      661 tcccgacatg atgagctcgg ccgccagcct cctgaggtcg gcggtcctgt cctcccaggt
      721 tacgatgtcg aagccggacc gggccagaca ttcttccagc cagtccctgc cgaggacttc
      781 catgcccggc ggcgcagccg acccgggaaa gagatgatac aggtcggaaa gaatgaggaa
      841 gccgccttct tccacgatcc tggagaactc cctcaccacc acctccgcgt tggtgtggga
      901 gagaacacac tcgcacagga cgccttgaaa ggcgccgttc ctgaagggaa tctcttccat
      961 tcttgcccgc acgagagaac cctccccatc ctcgtccctc gctgccgcaa gcatctccat
     1021 ggaaaggtcg aggccgcagc accgaacccc gtatcgttcc ctgagatgcc tgagcgtcac
     1081 accgttgccg caccccgcgt caaggacccg ggcctcggga ggcatacggc acagttcgag
     1141 agccctcgtc gtcagggtga gtccaccggg cctcaaggtc ccgcccgtca tcctctgaag
     1201 gcatgccctg cgttcatcat tctccatggc aggcgctact tgtcctcgag catcttctcg
     1261 gcctgggcca tcctctgagt ggcgtcctcc tcgcttacga atatgcgctg acaggaagga
     1321 cacgcaggta tgtcgacacc gaaggtctcc tccatgtatt tcagcttcac cgtggccagc
     1381 tcgagtctcc tgtcacactt catacaggtc cagtcaagcg tttgctgttt cttcgcgggc
     1441 atgttgaagc catgcagtat ctccatgcgg tgggaatagg ccctgtatat cctgtacgcc
     1501 ccgccgtccc ggccgaactc gacccaatag gtcgtcgtcg ccggcgtgaa gtaggacaga
     1561 agatgtcccg tgtccgggtg tatgtagaca tttcccgttt cctctccgaa atcaaggacc
     1621 ccttgaatgt cctcttccct tatgcccctc ttctcgagga cggcctgaac atctgcatcg
     1681 atcagcagtc ttttcccctc tttcaccatc cgctctcctt gccattaacg cgtttgtcca
     1741 ccccataccg gcggtggaca aacataatat ccatttattg tttcccaatc tcagacgcat
     1801 tcacgcctat tcacctggcc gccttcccgt gtccccgacc cgtacggcaa tatccccccc
     1861 gtgcaggcgg ggcatatgtc accc
//
LOCUS       DAOQTR010000463         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_80003_length_1880_cov_3.710137, whole genome shotgun sequence.
ACCESSION   DAOQTR010000463 DAOQTR010000000
VERSION     DAOQTR010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80003_length_1880_cov_3.710137"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="PKN85_09910"
     CDS             complement(<1..1398)
                     /locus_tag="PKN85_09910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNT44769.1"
                     /translation="MSGIKDGMAQKTREDENLYQVLFESSEIATIIAERDGTILRVNK
                     RFLELSGYRRSDVVGKKKWSELVVNGDLTKMTQYRDFLDHDASAPAHTYEFKLAAKNG
                     STRDVQILRQAVAGTKHAAIHFVDISDLMRVGKKVISSEEKYRSLIESTDDSIYMIDR
                     EGTYLFVNRQVLARLCVTEANIIGKHYSDFHDAEDTIEFAGYVDKVFETGSSHRYEHR
                     SGKGGDRWMLRTLSPIVEGRTGQVRFVAVVSKEITDLKKTEEKLKYLSLHDPLTNLYN
                     RAYFEEEMHRHDNNRFDVVGLIMCDIDGLKLVNDTLGHDRGDELLTVASQVIRKSFRE
                     SDVVARVGGDEFAILLPNSPKSKVEEICARIRTSVAAYNKKNPHLPLGISTGFAIRTG
                     PGQSMAELYREADNAMYKEKLFGSQHARNVIVKNLLNTIESKGVTTKQSLKRLRQLVS
                     VMGSAAGLTEKRLKDL"
     gene            complement(1433..>1880)
                     /locus_tag="PKN85_09915"
     CDS             complement(1433..>1880)
                     /locus_tag="PKN85_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT44770.1"
                     /translation="EAEAERLWTYRREVLPSLKALGKDHLEADVVVPRYNLPFLVRFT
                     GGLEHSDAIKIATYGHAGDGNLHVTILHRRRNFSELEEAYRLLELIYRETIAMGGSLT
                     GEHGVGLTVMDYLPMQIGGEALPLMRRIKAAFDPRGILNPGKIFTD"
BASE COUNT          328 a          544 c          550 g          458 t
ORIGIN      
        1 gaggtccttc aggcgcttct cggtcaggcc ggcggcgctt cccattacgc ttacgagctg
       61 tctcaaccgt ttgagggact gcttcgtcgt gacgccctta ctctcgatgg tgttcaggag
      121 gtttttcact atgacattgc gcgcgtgctg actgccgaag agcttttcct tgtacatggc
      181 gttgtccgct tcccgataca actccgccat gctctggccg ggaccggtgc ggatagcgaa
      241 ccccgtggaa atgcccaacg ggaggtgagg gttcttcttg ttgtacgcgg ccacgctggt
      301 cctgatcctg gcgcagatct cctcaacctt tgatttgggg ctgttcggca aaaggatagc
      361 gaactcgtcg cctccgacgc gcgcgacgac gtcgctttca cggaatgact tccggatgac
      421 ctgtgaggcg acggtgagga gttcgtctcc cctgtcgtgc ccgagggtgt cgttgacgag
      481 cttcaggccg tctatatcgc acatgatgag cccgaccacg tcgaaccggt tgttgtcgtg
      541 acgatgcatc tcctcctcga aataggcccg attgtagaga ttcgtcaggg gatcgtggag
      601 gctcaggtac ttcagtttct cttccgtttt cttgaggtcc gtgatctcct ttgacacgac
      661 ggcaacgaac cttacctgcc cggtgcggcc ttcgactatg gggctcagtg tgcgcagcat
      721 ccagcggtcc ccgcctttcc cgctcctgtg ttcataacga tgtgacgatc ccgtttcaaa
      781 gaccttgtcc acgtaccccg cgaactcgat ggtgtcttcg gcgtcatgga agtcgctgta
      841 gtgcttgccg atgatgttcg cctcggtcac gcacaaccgt gccagtacct gcctgttgac
      901 gaagagatag gtgccttcgc ggtcgatcat gtagatcgag tcatccgtgg actcgatgag
      961 ggaacggtat ttttcctcgc ttgagatgac cttcttgccg acgcgcatga gatcgctgat
     1021 gtccacgaag tgtatggccg cgtgcttcgt cccggcgacg gcctggcgga ggatctggac
     1081 gtccctggtg gaaccgtttt tggcagccaa cttgaattca taggtgtgag cgggagcgga
     1141 ggcgtcatgg tcaagaaagt cacggtactg tgtcatcttc gtgagatcgc cgttgacgac
     1201 gagttcggac catttcttct ttcccaccac gtcggaccgg cggtaccccg atagctccag
     1261 gaaccgcttg ttcacgcgta agatcgttcc gtctctctcc gcgataatgg tggcgatctc
     1321 cgaggattcg aaaagaacct ggtacagatt ttcgtcttcc cttgtcttct gtgccatgcc
     1381 gtctttgatg ccactcatta tatcaccggc gaacgggcat ttccaacact attcaatccg
     1441 tgaatatctt gccggggttc aggatgcccc gggggtcaaa ggccgccttg atgcgcctca
     1501 tgagcggcaa cgcctcccct ccgatctgca tggggaggta atccatgacg gttaggccga
     1561 caccgtgctc acccgtgagg gatccaccca tggcgatcgt ttccctgtat atcagttcca
     1621 ggaggcggta cgcctcttca agctcggaga agttcctccg gcggtggagg atggtgacat
     1681 ggaggttgcc gtccccggca tggccgtagg tggcgatctt tatggcatcg ctgtgttcga
     1741 gtccgcccgt gaacctgacg aggaacggga gattgtaacg ggggacgacg acgtccgcct
     1801 cgagatggtc cttgccgagc gccttgagcg atggcaacac ctccctgcgg taggtccaga
     1861 gacgctccgc ctccgcctcg
//
LOCUS       DAOQTR010000464         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_80007_length_1880_cov_3.469041, whole genome shotgun sequence.
ACCESSION   DAOQTR010000464 DAOQTR010000000
VERSION     DAOQTR010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80007_length_1880_cov_3.469041"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1018)
                     /locus_tag="PKN85_09920"
     CDS             complement(158..1018)
                     /locus_tag="PKN85_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44771.1"
                     /translation="MKKLSVALLLIFCAAAVLAGAGEVSAAVQAGYIFDIEGTAQLRS
                     ADGKTITLKRSEHILYAVKEGDRISVRKGRVVVASLKESKGYEIGDNSEGLAKPGRMV
                     AVRGKISELTGLYAPGGTSSGSMGGFVVRGVRPCIKAISPVSTTILDPAPVLSWENRC
                     SGDRKVTVKIISGDTVVYSAESEGSSLKVPDKVLAYGNEYRWIVDSGKVNNVSGGVFS
                     LPSEAEAEELGRKIAAFKERKEDLSFRLSYVFFLVDNNLNDLARAEIKTLKADFPENE
                     HLKKMEESIR"
     gene            complement(1077..>1880)
                     /locus_tag="PKN85_09925"
     CDS             complement(1077..>1880)
                     /locus_tag="PKN85_09925"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT44772.1"
                     /translation="IVGLTALFFAVNLLLFAAGSYVHIFPQALVPFVVAILVYPYRYL
                     VEERARRKVEKVFGYYMDRNVLDRLIETDPEALLAGEARDVSILFLDIRNFTVLSTQR
                     EARQVVRFLNSFFGAVTERIQERGGFVNKFIGDGLLAFFMTGQNPAADAVSAALNILD
                     ETRRLNDEGRFREFIGDWQVSVGIGIHHGEVILGNIGSHRKMDFTIIGEHVNIASRIE
                     GLTKEAGAALLVSSDARAAAADVFPWREAGEFALKGVDRPISLYTVGET"
BASE COUNT          362 a          596 c          532 g          390 t
ORIGIN      
        1 tcaaggttca tgtcgaggac cttgcaggga tggccggcgt cgcccaaggc gccggcgaga
       61 cgcgcgaggc ccgcgggagg ttcgcagggt ttggctacag ggggattgat aaggagcacg
      121 ttctttcttt caggcaggac gggaacgaca ttgagctcta cctgatgctt tcctccatct
      181 ttttcaaatg ttcgttctcg gggaaatcag ctttcagggt cttgatctcg gcacgcgcca
      241 gatcgttcag gttgttgtca acgaggaaga atacgtagga gagcctgaag gaaagatcct
      301 ctttgcgctc tttgaaggcg gctatcttcc ggccgagctc ctcagcctcc gcctcggacg
      361 gcagggagaa gacgcctccc gaaacgttgt tcactttgcc ggaatccact atccaccggt
      421 actcattccc ataggccagc accttatcgg gtaccttcag ggagcttccc tcgctctccg
      481 cgctgtacac gacggtgtcg ccggagatta tcttcaccgt caccttcctg tcgccggaac
      541 atctattctc ccaggagagc accggcgccg ggtcgagtat ggtggtgctc accggagata
      601 tcgctttgat acagggccgg acgccgcgca cgacaaagcc tcccatggac cccgatgagg
      661 tgcctcccgg ggcatagagg cccgtgagct ccgagatctt tcccctgacg gccaccatcc
      721 tgcccggttt cgccaatcct tccgagttgt cgccgatctc gtagcccttg ctctccttca
      781 gcgacgcgac gaccaccctg ccctttcgca cgctgatcct gtccccctcc ttgacggcat
      841 agaggatgtg ttcgctccgt ttgagggtga tcgtctttcc gtcagcgctc ctgagctgcg
      901 ctgtcccctc aatgtcgaag atgtaccctg cctggacggc cgcgctcacc tcgccggccc
      961 cggcaaggac agccgcggcg cagaagatca aaagaagggc cacagatagc tttttcatgt
     1021 tcttctcctt ctcctggaaa tatcctctcc tgttccccgc atgtacctac atcacatcac
     1081 gtctctccga cggtgtaaag tgaaatgggg cggtccaccc ccttgagggc aaattccccc
     1141 gcctccctcc acgggaagac gtccgctgcg gcggcgcggg cgtcgctgct cacgagaagc
     1201 gccgcgcccg cctcctttgt gaggccctct atgcgggagg cgatgttcac gtgctcgccg
     1261 atgatggtga agtccatctt cctgtgagag cctatgttgc cgaggatgac ctcgccatga
     1321 tgtatgccga tgccgacgct cacctgccaa tcgccgatga actccctgaa ccggccctcg
     1381 tcgttgaggc gcctcgtctc gtcgaggata ttaagggccg ccgacaccgc gtcggcggca
     1441 gggttctgac cggtcatgaa aaaggccagc aggccgtcac cgatgaactt gttcacgaaa
     1501 ccgccccgtt cctgtatcct ttcggtgacg gcgccgaaga aggaattgag gaaacgcacc
     1561 acctggcgtg cctccctctg cgtcgagagg accgtgaagt tccggatatc caggaacagt
     1621 atggaaacat cccgcgcctc gccggcaaga agggcctccg ggtccgtctc gatgagcctg
     1681 tcgagaacat tcctgtccat gtagtatccg aagaccttct ccaccttccg ccgcgccctt
     1741 tcctcaacaa gataccggta aggatacacg aggatggcaa cgacgaaggg caccagcgcc
     1801 tgggggaaaa tgtggacgta gctccccgcg gcaaagagga gaaggttgac ggcaaagaac
     1861 agggcggtga ggccgacaat
//
LOCUS       DAOQTR010000465         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_80694_length_1870_cov_3.368044, whole genome shotgun sequence.
ACCESSION   DAOQTR010000465 DAOQTR010000000
VERSION     DAOQTR010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80694_length_1870_cov_3.368044"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="PKN85_09930"
     CDS             complement(<1..1230)
                     /locus_tag="PKN85_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNT44773.1"
                     /translation="MKKDKKKKKKKKSSTGKIYKYLLVLLVASAVLFVAAFIFFIIND
                     IPSVVALKDLKNKPVTSVYDVNDELTYLFVPDNRVFVTYKRIPKHVRAAFLAAEDADF
                     FKHGGVDPMGILRAFIKNVAYGRMAQGGSTITQQVIKSMILGPEKSLNRKIREAILAY
                     KLENYLSKNEILNLYLNNIYMGHGVYGVEAASQVYFGKHVWNITPAEAALLAGIVQAP
                     SRYTPKRHPGNARMRQEYVIDQMVEKKFISEKAKQQMLKEKIDIREDDGVFADSYYKD
                     HILRYVEARYGKGVLSRKGLKIYAAVDRRMQKTAEDAIKRGLELYERRKGEYTVLYHL
                     GQKKWDDLKRAEERDRKLVPVSKGRTCSLLVSERIKEGYAVFVGEQKTVLKMDTHPFK
                     PDNVVKGFCTGEDRKKTP"
     gene            complement(1227..>1870)
                     /locus_tag="PKN85_09935"
     CDS             complement(1227..>1870)
                     /locus_tag="PKN85_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HNT44774.1"
                     /translation="RRPSPELEVGVEFYPYAFSSVGASEFVVERPDVKTVPVIDIVDR
                     TVTRRMDVALSRHGNIILPDRKGDVRKIMYTRREQKKWGQGFLHGFGADCGGIASTVA
                     HETHGLLVHGFDDDDMALAANTVLAMKGGIAVVDAGKVLHALRLPLGAIMSRVPLPEL
                     DRALRKLVDLLHSLGNTLDDPLWTMGFLPFTSIVELRITVSGTYDVRKGSIVF"
BASE COUNT          351 a          561 c          507 g          451 t
ORIGIN      
        1 cggcgtcttc ttcctgtcct caccggtgca aaaccctttc accacattgt cgggtttgaa
       61 agggtgggtg tccatcttga ggacggtctt ctgctcgcct acaaagacgg cgtacccttc
      121 ctttatcctt tccgatacca ggagactgca ggtccgtccc ttgctgacgg gcaccagttt
      181 gcggtcgcgc tcttccgccc gcttcaggtc atcccacttc ttctgcccca ggtggtagag
      241 gaccgtgtat tcgccctttc tccgttcata gagctcgaga cccctcttta tcgcgtcctc
      301 ggcggtcttt tgcatccgtc tgtccacggc cgcgtagatc ttgagtccct tcctggacag
      361 aacgcctttg ccgtagcggg cctccacata ccggagtatg tggtccttgt agtagctgtc
      421 ggcaaagacg ccgtcgtcct ccctgatatc gatcttttcc ttgagcatct gctgctttgc
      481 cttctcgctg atgaacttct tctccaccat ctggtcgata acgtattcct gtctcatccg
      541 ggcatttccc ggatgccttt tgggtgtata ccgggagggc gcctgcacga tacctgccag
      601 cagggccgcc tcggcgggcg ttatgttcca gacatgcttt ccgaagtaca cctgggacgc
      661 ggcctccacg ccatagacac cgtgtcccat gtagatgttg ttgaggtaaa gattgaggat
      721 ctcgttcttg ctcaggtagt tctccagctt gtatgccagg atggcctcgc gtatcttcct
      781 gttcaggctc ttttcaggac ccagtatcat tgacttgatg acctgctgtg tgatggtgct
      841 gcccccctgg gccatccggc cgtatgccac gttcttgatg aatgccctta ggatgcccat
      901 cgggtcgacg cccccgtgct tgaagaaatc ggcgtcctcc gccgccaaaa aggcggccct
      961 cacatgcttt gggatccgtt tgtaggtaac gaagaccctg ttgtcgggta caaacaggta
     1021 ggtgagttcg tcattgacgt cgtaaacgct ggtgacgggc ttgttcttga ggtccttcag
     1081 cgccacgacg gagggtatat cgttgataat aaagaagatg aaggccgcga cgaagagcac
     1141 cgccgacgcg acgagcagga ccagcagata cttgtagatc tttcccgtcg aactcttctt
     1201 cttctttttt ttcttcttgt ccttcttcaa aacactatcg aaccttttct gacatcatag
     1261 gttcccgaaa cggtgatcct cagctccacg atggaggtga aaggcagaaa gcccatggtc
     1321 cagagggggt catcaagggt gttcccgaga gagtggagaa ggtcaacgag ctttcgaagg
     1381 gcacggtcca gttcgggcaa ggggacgcgg gacatgatgg cgccgagcgg cagccgcagg
     1441 gcatgaagaa ccttgccggc gtcaacgacg gctatccccc ctttcatggc gagcaccgtg
     1501 ttcgccgcca gagccatgtc atcgtcatcg aaaccgtgaa cgaggagacc atgcgtttca
     1561 tgggccaccg ttgaggcaat acccccgcag tcggccccga agccatgaag gaacccctgg
     1621 ccccacttct tctgctccct ccgcgtgtac atgatcttcc gtacgtcgcc tttcctgtcg
     1681 gggagaatga tattgccgtg tcgggacagc gcgacatcca tccgcctcgt cacggtgcgg
     1741 tcgacaatat ctatgacggg aacggtctta acgtccggcc gctccacgac gaactcggag
     1801 gccccaacgg acgagaacgc gtaagggtag aactctaccc cgacctcgag ctccggtgac
     1861 ggccgacgcg
//
LOCUS       DAOQTR010000466         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_81118_length_1864_cov_4.864013, whole genome shotgun sequence.
ACCESSION   DAOQTR010000466 DAOQTR010000000
VERSION     DAOQTR010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81118_length_1864_cov_4.864013"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PKN85_09940"
     CDS             <1..1780
                     /locus_tag="PKN85_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44775.1"
                     /translation="CRSTGYAREEILDLALPVIDPSFAPPVWNDHWNDVKERGTIVFE
                     SFQKRKDGSLYPVEIKGNYMEYGSIGYICSIAFDITERKKTDELLRMTSFSMDQSAIP
                     TFWLDMDGNALRVNKAALISLGYTEEEMLGKNVRTWDEDFPHDLWDSHIFPKLKARHS
                     LTMESKCKRKNGSKFPVELNLNYMTYAGNEYVFVFAHNISERKRADEERERLQAQLLQ
                     SQKMEAIGQLAGGVAHDFNNILTAVIGYGNLLEMEMEQEHPLRAYVEEILASSEKAVN
                     LTQSLLAFSRKQSINLKHHDINDIISGVENLLKRLLSEDIDLKISLTSPGITIMADIT
                     QIQQVLINLATNARDAMPSGGMLSIEAGHVRPNDSFLRSQGGKGSLYAMISVSDTGSG
                     MDEQIRARIFDPFFTTKEVGKGTGLGLSIVYGIVKQHNGFISAYSEKDAGTTFHIYLP
                     AVEERTREGKVKPSVASKGYGTILLAEDNPEVRRLATSVLRKTGYSVIEAADGQEAIE
                     AFMANQDAIDLIIIDVVMPRKNGREVVDRVREVRPDARVLYTSGYTRDILSDKGMDDT
                     SFAFLAKPLSPHELLQKVQEILTTTA"
BASE COUNT          462 a          553 c          516 g          333 t
ORIGIN      
        1 ctgccgctcc acgggttacg cgcgggagga gatactggac ctcgcccttc ccgtcatcga
       61 cccctccttc gcgccccccg tctggaacga ccactggaac gacgttaagg agcggggaac
      121 gatcgtcttc gagtcgtttc agaagcgcaa ggacggctct ctttatcccg tcgagatcaa
      181 gggaaactac atggaatacg gcagcatcgg ctacatctgc tccattgcct tcgacattac
      241 ggagagaaag aagaccgatg aacttctccg catgaccagc ttttccatgg accaatcggc
      301 gataccgaca ttctggctcg acatggacgg caatgccttg cgcgtcaaca aggcggccct
      361 tatttccctc ggatacacgg aggaggagat gctcggcaag aacgtcagaa cctgggacga
      421 ggatttcccc catgacctct gggacagcca catcttcccg aaactcaaag caaggcattc
      481 cctgacgatg gaatcgaagt gcaagaggaa gaacgggagc aagttccccg tggagctgaa
      541 tctgaactac atgacatatg cgggcaacga atatgtcttt gtctttgccc acaacatcag
      601 cgagagaaag cgggccgacg aggaaagaga gaggctgcag gcccagctcc tccagtccca
      661 gaagatggag gccatcggcc agctcgctgg cggtgtcgcg cacgatttca ataatatcct
      721 caccgccgtg atcggttacg ggaacctcct ggagatggaa atggaacagg aacacccgct
      781 gcgcgcctac gtcgaggaga tcctggcttc ctcggagaag gccgtcaacc ttacccagag
      841 ccttctcgcc ttcagcagaa agcagagcat caatctcaag catcacgata tcaatgacat
      901 catctccgga gtcgagaacc ttctgaagcg gcttctctcg gaggacatcg acctcaagat
      961 atcgctgacg agcccgggca tcacgatcat ggcggacatc acgcagatcc agcaggtgct
     1021 catcaacctc gccaccaatg cccgggacgc catgccgtcg ggcggtatgc tttccatcga
     1081 agccgggcat gtccggccca atgacagttt cctcaggtcc cagggcggaa agggctcgct
     1141 ctacgcgatg atctccgtct ctgacacggg cagcggtatg gacgagcaga tccgggcgcg
     1201 catcttcgat cctttcttca cgaccaagga ggtgggaaag ggcaccgggc tgggactgtc
     1261 catcgtgtac ggcatagtga agcagcacaa cggcttcatt tccgcttatt ccgaaaagga
     1321 cgcggggaca acgtttcaca tctacctgcc cgccgttgag gaaaggaccc gcgagggcaa
     1381 ggtgaagcct tccgtggcaa gcaaggggta cggaacgatc ctcctcgccg aagacaaccc
     1441 cgaggtccgt cggctggcaa ccagcgtcct caggaagacc ggctatagcg tcatcgaagc
     1501 cgccgacgga caggaagcca tcgaggcctt catggccaat caggacgcca tcgacctcat
     1561 catcatcgat gtggtcatgc cccggaagaa cggaagggaa gtggtcgatc gggtcagaga
     1621 ggtccgaccc gatgcgaggg tcctctatac aagcggatat acgagagaca tactctccga
     1681 caagggcatg gacgacacga gcttcgcctt cctggcaaaa cctctctccc cccatgaatt
     1741 gcttcagaaa gtccaggaaa tactgaccac caccgcttga acccaaaaag atggttgcag
     1801 ccgcttgagc cgcttgagcg ttaaggaata aagaaagaac ataaaagtac ggcccggctc
     1861 ccgg
//
LOCUS       DAOQTR010000467         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_81957_length_1852_cov_3.462994, whole genome shotgun sequence.
ACCESSION   DAOQTR010000467 DAOQTR010000000
VERSION     DAOQTR010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81957_length_1852_cov_3.462994"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /gene="amrB"
                     /locus_tag="PKN85_09945"
     CDS             <1..229
                     /gene="amrB"
                     /locus_tag="PKN85_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT44776.1"
                     /translation="AVTVEHLRNFDIGAMIRDTRSEKAQACGAGPMITAMMAARKLGA
                     GTGRVLKYANSGDVSGDRSGVVGYVSAIFFE"
     gene            250..783
                     /gene="amrA"
                     /locus_tag="PKN85_09950"
     CDS             250..783
                     /gene="amrA"
                     /locus_tag="PKN85_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNT44777.1"
                     /translation="MALSAEEKQKLKKLVKDAIEGVLFGREAIPVEVTESLEEKCGAF
                     VTVRNCGNLRGCIGYVRGYLPLHETVREMAIQAAFNDPRFDPVTRDEWKDVDFEISVL
                     TPMKKIEDVSEIEVGVHGLYIEKGVHSGLLLPQVATEQKWDRTEFLEYTCYKAGLPKD
                     AWKSKDADIFIFSADVF"
     gene            829..1041
                     /locus_tag="PKN85_09955"
     CDS             829..1041
                     /locus_tag="PKN85_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNT44778.1"
                     /translation="MIDKRTSIREVLAKHPETARVFEEFGLGCAGCEAALFESVEQGA
                     QVHGVDVDSLIEGLRKTMGEGWNSER"
     gene            1057..1671
                     /locus_tag="PKN85_09960"
     CDS             1057..1671
                     /locus_tag="PKN85_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT44779.1"
                     /translation="MTGIYSVILAAGSSKRLGFNKLTLLMDSEPVIRRTATPFVEAGL
                     GGEVIVVAGSDTVPVAAALEGLNVRVVRNEDHRQGMSSSIRAALPWIAEATAVFFHLG
                     DKPLVKKELLIAMVDRYRETRRGIIVPVHDGMKGHPVLMSHGPYREEMGSLDGDKGLR
                     EVIEKHSADVLFIEGDEGVIFDIDTGEDLEALRRRGYRVEKGAF"
BASE COUNT          410 a          445 c          639 g          358 t
ORIGIN      
        1 cgccgtcacg gtggagcatc tcaggaactt cgatatcggg gccatgatca gggacacacg
       61 gtctgagaaa gcgcaggcgt gcggggcggg accgatgatc accgccatga tggctgcccg
      121 gaagcttggg gccgggactg gcagggtact gaagtacgca aattcgggcg acgtgtcggg
      181 ggaccgcagc ggtgtcgtgg ggtatgtgtc ggcgatattc tttgaataat gactggcggg
      241 gatggatgaa tggcactttc agcagaagaa aagcagaaac tcaagaagct cgtcaaggac
      301 gcgatcgaag gtgttctctt tggcagggaa gcgatccccg ttgaggtgac ggaatccctg
      361 gaggagaagt gcggcgcctt cgtaactgtc aggaactgcg gcaatctgag gggatgcata
      421 ggttacgtga ggggttatct tccacttcac gagaccgtca gggagatggc gatccaggcg
      481 gccttcaacg atcccaggtt cgatcccgtc acccgcgacg agtggaagga cgtcgatttt
      541 gagatctcgg tcctgacacc gatgaagaag atagaggacg tgagcgaaat agaggtcggc
      601 gtccatggcc tgtacataga gaagggcgtg cattcggggc tcttgcttcc ccaggtggcg
      661 acggaacaga aatgggacag gacggagttc ctggagtaca cgtgctacaa ggcgggtctt
      721 ccgaaagacg cgtggaaatc gaaggacgcc gatatcttca tcttttccgc ggatgttttt
      781 tgaacagcgg aaccagcgtg atttccgaga cagggccgga gggaaggaat gatcgacaaa
      841 aggacgtcca ttcgggaggt cctcgcgaag catccggaga ctgccagggt attcgaggag
      901 ttcggcctgg gatgtgccgg atgcgaggcc gcgctcttcg agagtgttga gcagggagcg
      961 caggtccacg gtgttgacgt ggatagtctc atcgagggcc tccgcaagac aatgggggaa
     1021 ggctggaaca gtgagcgttg acggaattca tatctcatga caggcatcta ctccgtgata
     1081 ctcgccgcgg gctcatcgaa gaggctcggc ttcaacaagc tgaccctgct catggattcc
     1141 gagccagtca tccgcaggac cgcgacccct ttcgtggagg ctggtcttgg cggtgaggtc
     1201 atagttgttg ccgggagcga taccgtccct gtcgcggcgg ccctcgaagg cttgaatgtg
     1261 agggtggtgc ggaatgaaga ccaccgtcag gggatgtcga gctccatcag ggcggccctg
     1321 ccgtggatag cggaggcgac ggcggtcttc tttcacctcg gggataagcc tttggtgaag
     1381 aaggaactcc tgatcgccat ggtcgaccgg tatcgtgaaa cgcgcagggg catcatcgtt
     1441 cccgtccatg acggaatgaa gggccacccg gtccttatga gccacgggcc ctacagggag
     1501 gagatgggat ctctcgatgg agacaaaggc ctccgtgaag ttatagaaaa gcacagcgca
     1561 gatgtgctat ttatagaagg cgatgaaggc gtcattttcg atatcgatac gggagaggat
     1621 ctggaggccc tgagacgacg gggatacagg gtcgaaaaag gcgcgttctg agacagccga
     1681 cagaggaggt tcaaggcggt gccgttcaag cgaaagagta ttggcgagct gtccgtggtc
     1741 ataaaggggg ccggtgagat ggcctcgggt atcgcccaca ggcttcaccg ggccgggata
     1801 cggcgtgtcc tcatgctcga tatgccgagg cccctgtgtg tccggcggtt cg
//
LOCUS       DAOQTR010000468         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_82124_length_1850_cov_2.900279, whole genome shotgun sequence.
ACCESSION   DAOQTR010000468 DAOQTR010000000
VERSION     DAOQTR010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82124_length_1850_cov_2.900279"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKN85_09965"
     CDS             <1..358
                     /locus_tag="PKN85_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44780.1"
                     /translation="VTLILSDERYMYLASELMGGEKVFVPHWDRLCRMGRDRRTRRLA
                     LHRLGKAPSQLFMEGVRGMAEALKGPLTSLWYLCAGACFYAFIIYLIWPDSKAGRWIA
                     ATVKRILAEATRMIYD"
     gene            413..>1850
                     /locus_tag="PKN85_09970"
     CDS             413..>1850
                     /locus_tag="PKN85_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44781.1"
                     /translation="MTPYRKLGDDMDAVIRSFDTNPAVTEWQRNTWRPEGKKKAITHD
                     MFGCDHEKALAFEDGTRIGSLKKNSARRFWDYAGWHVFPPVEDVVRFCLFMHLDLYRT
                     VALLLKAKWERFFAHEVNGWRASDGTSLTDILLDRDEHALREALARFQPREGLYNLIG
                     DLAARHAPASDTGRPFLEGHTPSTFGELVKEMLMGRYHFLSVLSAEVSQFAARARNEF
                     LLLVEGTKKEQQTYAIEKARWVSLRQELEDVYLLIENQRIRNAHTRREWLALFGKEEI
                     GLREAALILERCDMRLNLKKVNPDWSREDIEEYIEREEAQRQLELTRLKTDAALAPHL
                     VRISGETGAGGKAVEADRYLKECKTVLRQIRRLLHPDRLMHHPSYAHLTEGQKERLEE
                     MLLSALEVRPEELGYPEGYILHDMRSLEGLKSALTRVEAILGNPGVEIDERLTIQGET
                     LPEKLTWLQREVRLLEDEVLAAKAELQAL"
BASE COUNT          409 a          543 c          579 g          319 t
ORIGIN      
        1 cgtcaccctc atactctccg acgagcggta tatgtacctg gcgagcgagc tgatgggcgg
       61 agaaaaggtg ttcgtcccgc actgggaccg cctctgccgg atgggtaggg acagaaggac
      121 acgaaggttg gcccttcatc gccttggcaa agccccttct cagcttttca tggagggtgt
      181 acgcgggatg gcggaagcgc tgaaaggtcc gctcacgtct ctctggtatc tctgcgccgg
      241 tgcatgcttt tacgcgttta tcatctacct catctggccg gactcgaagg cgggcaggtg
      301 gatcgccgcg acggtaaaga gaattctcgc ggaagcgaca agaatgatat acgattagaa
      361 ggaaaccgct catttgcgac cctcttccgc tgaaaaggaa aggagataga aaatgacacc
      421 ctacaggaaa ctcggcgacg acatggacgc ggtcatacgg tccttcgata cgaaccccgc
      481 cgtcacggaa tggcaaagga acacctggcg gccggagggc aagaagaagg cgatcaccca
      541 tgacatgttc gggtgcgacc acgagaaggc ccttgccttc gaagacggga caagaatagg
      601 ttccctgaag aagaacagcg cccgccggtt ctgggactat gcgggatggc atgtcttccc
      661 gccggtagag gacgtcgtcc gtttctgcct tttcatgcat ctcgaccttt accggacggt
      721 ggcgctcctg ctgaaagcca aatgggaacg ctttttcgcc cacgaggtga acggctggag
      781 ggcgtcagac gggacaagcc ttaccgatat ccttctcgac agggacgagc acgccctgag
      841 ggaggccctt gcccggttcc aaccccggga gggcctctac aacctcatcg gggaccttgc
      901 cgcccgccac gcgccggcgt ccgacaccgg caggcctttt ctcgaggggc acacgccttc
      961 caccttcggg gaactggtga aagagatgct catgggaagg tatcatttcc tttcggtatt
     1021 gagcgcggag gtctcccaat ttgcggcgcg ggcacggaat gagttcctcc ttctcgtgga
     1081 aggcaccaaa aaagagcagc agacctacgc catcgagaag gcgcggtggg tatctctgcg
     1141 gcaggaactg gaagacgtct acctgctcat cgagaaccag cggatcagga acgcccacac
     1201 ccgccgcgag tggctcgccc tcttcggaaa ggaagagatc ggactcaggg aggctgccct
     1261 catactcgaa cggtgcgata tgcggcttaa cctcaagaaa gtgaaccccg actggtcccg
     1321 tgaggatatc gaagagtaca tcgagaggga agaggcgcaa aggcagctcg agctcacgcg
     1381 gcttaagacc gacgcggcgc tggcccccca cctcgtgagg atctccgggg agacgggcgc
     1441 gggcggaaag gcagtggagg ccgacaggta cctgaaagag tgcaagacgg tgctccggca
     1501 gatacgcagg ctcctccacc ccgacaggct catgcaccac ccctcttacg cgcatctcac
     1561 agagggccag aaggaacgcc ttgaggagat gctgctttcc gccctcgagg tccggcccga
     1621 ggagctcggc tatcccgagg ggtacatact tcacgacatg cgctccctcg agggtttgaa
     1681 gagcgccctc acgcgcgtcg aggccatcct gggcaacccg ggggtcgaga tcgacgagcg
     1741 cctcaccatc cagggtgaaa ccctgccgga aaagctcacg tggctgcaaa gggaagtgag
     1801 gttgctcgag gatgaggtcc tcgccgcgaa ggcggagctg caggcgctct
//
LOCUS       DAOQTR010000469         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_82272_length_1848_cov_3.341885, whole genome shotgun sequence.
ACCESSION   DAOQTR010000469 DAOQTR010000000
VERSION     DAOQTR010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82272_length_1848_cov_3.341885"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKN85_09975"
     CDS             <1..670
                     /locus_tag="PKN85_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44782.1"
                     /translation="TFFYNTYTELPIHAVQGNLDKIIPLYIDRFYPQWFVALFLVALM
                     SAAMSAGSSQFHALGTSLSRDIFEQALLKGKSVAETTIVTRVGIVFSIFATLIIGLVL
                     PESIVAVATAFFFSLCGATFIPVYLFGLYWRRGTKPAAKASILSGFFVSLVWMLLFHE
                     QEAKAFGLCRKLFNMETLAGHPWNMIDPQMIALPIGLIVFVVVSLATRPVREETVKKA
                     FVHI"
     gene            793..1050
                     /locus_tag="PKN85_09980"
     CDS             793..1050
                     /locus_tag="PKN85_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44783.1"
                     /translation="MGKRYVLGIGLSNRVENAADFQRVLTECGCYIKTRLGLHEVNEN
                     LCAPGGLIILEVYGGDAEVVDMESKLKAVKGLQVQKMVFDI"
     gene            1229..>1848
                     /locus_tag="PKN85_09985"
     CDS             1229..>1848
                     /locus_tag="PKN85_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44784.1"
                     /translation="MRDPDRNGTPTASFPLVIAFFLTAALCSFILPLNACLAGDLKEV
                     ALYSTPLTEATKASLEQHWPGTSQLILVRNDGPLPHNSILYAFLGKDRDWTLALGPIP
                     ATTGRNGFAKPLGKREGDGRTPVGVYPLGFAFGYPPGIDSAMPYRQMTRQDIWVDDPR
                     SPDYNRLKKRAETAARSFEDMVLPDDRYKHGIVIEYNTHPIIPGLG"
BASE COUNT          398 a          562 c          490 g          398 t
ORIGIN      
        1 cacgttcttc tacaacacgt acacggaact gcccatacat gccgtgcagg gaaacctgga
       61 caagataatc cccctttata tcgaccgctt ctatccccag tggttcgtgg cgctcttcct
      121 cgtcgccctc atgtccgccg ccatgagcgc gggaagcagc cagttccacg cgctcggcac
      181 gtccctctcc cgcgacatct tcgagcaggc ccttctgaaa ggaaagtcgg tggcggagac
      241 aacgatagtg acgagggtag gcatcgtctt cagcatcttt gccaccctca tcatcggcct
      301 cgtcctgccc gagagcatcg tcgccgtcgc gacggcattc ttcttcagcc tctgcggagc
      361 caccttcatc cccgtgtacc tcttcggcct ttactggagg cggggcacca aaccggcagc
      421 caaggccagc atactgtccg gttttttcgt atccctcgtc tggatgcttc tcttccacga
      481 gcaggaggca aaggccttcg gcctgtgccg aaagctcttc aatatggaaa cccttgctgg
      541 ccacccctgg aacatgatcg acccccagat gatagctctg cccataggtc ttatagtctt
      601 cgtcgtcgtg tccctggcga cgcgcccggt gcgggaggaa acggtgaaaa aggcatttgt
      661 gcatatttga gagtgatagg acgaatagga gagataggac ctataataca cataagacca
      721 ataggacgta tcgagggttc ttccctgaga ctcttgaact cttgaacgtt ctttattaga
      781 gaggagggtg ttatgggtaa gcgttatgtt ttgggtatag ggcttagcaa cagggttgag
      841 aatgcggccg actttcagag ggtactaact gaatgcggct gctacatcaa gacccgcctc
      901 ggcctccatg aggtcaacga gaacctctgc gccccgggag ggcttatcat cctggaggtg
      961 tacggaggcg acgcggaggt ggtcgacatg gaatcgaagc tcaaggccgt caagggcctg
     1021 caggtccaga agatggtgtt cgacatctga aagagaaagc agacgaccat acgggagaac
     1081 aaagaaggtc tgccttatta agaaacgggt ccctccggac ggtgcaggcc ttcctggcaa
     1141 ccgggtctgt tccggcctca ggccggaaca gcgcgataga catcatgcct tgcccattgc
     1201 aggccatggg tctttctgga gaccatagat gagggatccc gacaggaacg ggactccaac
     1261 ggcatccttc cctctcgtga tcgcgttctt tttgactgct gcgctttgca gtttcatcct
     1321 tcccctgaac gcctgccttg ccggcgacct taaggaggtg gctttgtatt ccacaccact
     1381 caccgaggcg accaaagctt ccttggaaca acactggccc ggcacttctc aactcatcct
     1441 tgtcagaaat gacggaccat tgcctcataa ttccattctc tacgcttttc tagggaagga
     1501 ccgagactgg acccttgctc tcggtccaat ccctgccacg acgggtcgaa atggctttgc
     1561 caagccgctc ggcaaacggg agggcgacgg caggacaccc gtcggagtat atcctctcgg
     1621 cttcgcattc ggctatcctc caggtatcga ttccgctatg ccctaccgcc agatgacccg
     1681 tcaggacatc tgggtcgacg acccccgctc tcccgattac aaccggctca agaaacgcgc
     1741 ggaaacagcg gcgcgctcct tcgaggacat ggtcctcccc gacgaccgtt acaagcacgg
     1801 catcgtcatc gagtacaaca cccaccccat catccccggc ctcggcag
//
LOCUS       DAOQTR010000470         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_82486_length_1845_cov_2.730168, whole genome shotgun sequence.
ACCESSION   DAOQTR010000470 DAOQTR010000000
VERSION     DAOQTR010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82486_length_1845_cov_2.730168"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>1845
                     /gene="rpoC"
                     /locus_tag="PKN85_09990"
     CDS             61..>1845
                     /gene="rpoC"
                     /locus_tag="PKN85_09990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNT44785.1"
                     /translation="MAIKISLASPELIEKWSYGEVKKPETINYRTFKPERDGLFCAKI
                     FGPVKDYECICGKYKRMKHRGIICEKCGVEVIQSKVRRERMGHIKLACPVAHIWFLKS
                     MPSKIGTLLELTIKKVERVLYFEMYIVIETGDSPYSFCEIISEEKYIEAREKYGEGFV
                     GGIGAEAIRECLKKINVDELGRTLRIDMRDTASDAKKKKIARRLKVVDAFKVSGVRPE
                     WMILDIVPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELNAPEIIIR
                     NEKRMLQEAVDALFDNSKRGRVVTGASKRPLKSLSDMLRGKQGRFRQNLLGKRVDYSG
                     RSVIVVGPELRLHQCGLPKYMALELFKPFVYNRLIRKGYATTIKNAKKIVEKERPEVW
                     DVLEEVIKEHPVLLNRAPTLHRLGIQAFEPQLIDGKAIQLHPLVCPAYNADFDGDQMA
                     VHVPLSTEAQTEARVLMMSTNNILSPANGKPIIVPTQDIVLGLYYLTIDRKYRRGENK
                     IFADSEEVRVAYDSGEIDLHARVRVRLNGEMVNTTPGRIILRDVVQDALMELPQPKRD
                     TVLRDMFSNINTIMDKKTIAALVDKCYRD"
BASE COUNT          459 a          525 c          538 g          323 t
ORIGIN      
        1 ggaggaacaa ccttggaaga atattttaag tcagacaagc agcgggaccc gttgagctac
       61 atggcgatca agatctccct ggcatcgccg gagctgatcg agaaatggtc ctatggggaa
      121 gtgaagaagc ccgaaaccat aaactaccgc accttcaaac ccgaacggga cggccttttc
      181 tgcgccaaga tattcggtcc cgtcaaggac tatgaatgca tctgcggcaa gtacaagagg
      241 atgaaacatc gcggcatcat ctgcgaaaag tgcggtgtcg aggttatcca gtccaaggtg
      301 cgccgcgagc gtatgggaca catcaagctt gcctgtcccg tggcccacat ctggtttctg
      361 aagagcatgc cgagcaagat cggcacgctt ctggaactca ccataaagaa agtggaacgg
      421 gtcctctact tcgagatgta catcgttatc gagaccggcg attcccctta cagcttctgc
      481 gagatcataa gcgaggagaa gtatatcgag gcccgcgaga aatacggcga gggttttgtc
      541 ggcggtatcg gggcggaggc aatcagggaa tgcctcaaga agatcaatgt cgacgagctt
      601 ggcaggacgc tgcggatcga catgcgcgac acggcgagcg acgcgaagaa gaagaagata
      661 gcgcggcgac tgaaagtggt cgacgccttc aaggtctccg gtgtaaggcc ggagtggatg
      721 atactcgata tcgttcccgt tttgcccccg gagctgaggc cgctcgttcc cctcgacggc
      781 gggcgtttcg ccacgagcga cctcaacgat ctctaccggc gcgtcatcaa caggaacaac
      841 cgcctcaaga gactcatgga actcaacgcc ccggagatca tcataagaaa cgagaaaagg
      901 atgctccagg aggcggtcga cgccctcttc gacaactcga agcggggcag ggtcgtcacc
      961 ggcgcgagca agcgtcccct gaaatcactg agcgatatgc tccgcggcaa acagggaagg
     1021 ttccggcaga acctcctggg taaacgggtc gactattcag gccgctccgt catcgttgtc
     1081 ggtcccgagc tgaggctgca ccagtgcgga cttcccaaat acatggcatt ggagctcttc
     1141 aagcctttcg tgtataatcg ccttatcaga aaaggctacg caacgacgat caagaacgcg
     1201 aagaagatcg tcgaaaagga aagacccgag gtctgggatg tcctggaaga ggtgatcaag
     1261 gagcaccccg ttcttctcaa cagggccccc acgctccacc gcctcggcat acaggccttt
     1321 gagccgcagc tcatcgacgg caaggccatc cagctccacc ccctcgtctg ccccgcgtac
     1381 aacgcggact tcgacggcga ccagatggcg gtgcacgtgc cgctgtccac ggaggcgcag
     1441 accgaggcca gggtgctcat gatgtcgacg aacaacatcc tttcccccgc caacggcaag
     1501 cccatcattg ttcccacgca ggacatagtg ctcgggcttt actacctcac catcgaccgg
     1561 aaatatcgca ggggagagaa caagatattc gccgattccg aagaggtccg agtggcctac
     1621 gactcgggag agatcgacct ccacgcgagg gtcagggtgc gcctcaacgg cgagatggtg
     1681 aacaccacgc cggggaggat aatcttgagg gatgtcgtgc aggacgctct catggagctg
     1741 ccccagccga agagggacac ggtcctcagg gacatgttct cgaacatcaa tacgatcatg
     1801 gacaagaaga ccatcgcggc cctggtggat aagtgctacc gcgac
//
LOCUS       DAOQTR010000471         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_82959_length_1838_cov_4.270892, whole genome shotgun sequence.
ACCESSION   DAOQTR010000471 DAOQTR010000000
VERSION     DAOQTR010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82959_length_1838_cov_4.270892"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PKN85_09995"
     CDS             complement(<1..876)
                     /locus_tag="PKN85_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44786.1"
                     /translation="MGGVDMVGKQIVIGFTLLVLLVAGTALIPAEYPPDDGGTNGGIE
                     IVGPYGVQGVSEITLGTALMWYPEQTMVYRAVDTIISRDDALKIAERFGMSGPVEESG
                     GRIKCYVVKDEPYTFEIEKEAGYMTYTWNDRYSGVDPRDRPENLPTDEECRKIAEAFL
                     TSHDLLPEGAVFSSVSQGGKGTFSNHSGGAPIVTYEDRDVLFTDTLSGLPVTGSGIRV
                     TVGGGGDILKVTKRWRDVEPYKEFPILPPEEALEELKQTGVVTAVSSPKRAIVGEVRL
                     CYYATPPGTEQPYLKP"
     gene            complement(864..>1838)
                     /locus_tag="PKN85_10000"
     CDS             complement(864..>1838)
                     /locus_tag="PKN85_10000"
                     /inference="COORDINATES: protein motif:HMM:NF039734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HNT44787.1"
                     /translation="EQTGVTNHLYSGWNVVGFWDVHQACARDMLLPLGSTWATAQGYD
                     AGTQDYEVSMINGGTGSYSDQRLMFPGKAYWVSMTAERDLICSYYRTYSFCAEWVGDY
                     HGMQIPITWADEEAEGFYNTLDWSSSWTGQFINGDDAAMERHWKDPAFGGMDSNYIDN
                     THLAFFTGHGWEGGFVFGTAADDYELNYSEARWGNTKTDWIVLASCNVLNESTCTEYW
                     GPAFEGLHSICGFDTVGAAHPDMGWYFADLLMKGKTIWEAWYTTTDRYVFPNDGSLKS
                     AILAADIDGDLSTPDCLDDHIYGYGSSINPPGDPLGFQYETDSCKWEV"
BASE COUNT          353 a          570 c          498 g          417 t
ORIGIN      
        1 cggcttgagg taaggctgct cggtcccggg cggggttgca tagtagcaga gcctgacctc
       61 gccaacgatc gcccttttcg gtgaactcac cgcggtgacg acaccggtct gtttgagttc
      121 ctcgagcgcc tcttcggggg ggaggatcgg aaattcctta tagggttcga catcgcgcca
      181 gcgtttggtg actttaagga tgtcaccgcc cccgccgacg gtgacccgga tcccgcttcc
      241 ggtcacggga aggccgctca aggtatcggt gaagaggaca tcccgatctt cgtacgtgac
      301 gatgggtgcc cctcccgaat ggttcgagaa agtccccttc ccaccttgag acacacttga
      361 aaagaccgcc ccttcgggca ggagatcgtg ggaagtgagg aatgcttcgg cgatcttccg
      421 gcactcttcg tcggtcggga ggttttcggg acggtccctt ggatctaccc cggagtagcg
      481 gtcattccac gtgtaggtca tatacccggc ctccttctcg atctcgaacg tgtagggttc
      541 gtctttgacg acataacact tgatccttcc tccactttcc tcgaccggtc cagacatacc
      601 gaacctctcc gctatcttca gcgcgtcatc cctcgatatg atggtatcga ccgccctgta
      661 gaccatggtc tgttcgggat accacatgag ggcagtaccg agagtgatct cagagacgcc
      721 ctgcacgccg tacggtccga caatctcgat ccctccgttt gtgccgccat cgtccggcgg
      781 atattccgcc ggtatcagcg ccgtcccggc aacgagcagc acgaggaggg taaatccgat
      841 tacaatctgt tttcctacca tatctacacc tcccatttac atgagtccgt ttcgtactgg
      901 aaacccaacg gatcgccggg tgggttgatc gatgatccgt atccgtaaat atggtcgtcg
      961 agacagtcag gtgtgctcag atctccgtcg atgtccgcgg caagaatcgc tgatttcagg
     1021 ctgccgtcat ttggaaagac atacctatca gttgtcgtgt accatgcttc ccagattgtt
     1081 ttgcctttca tcagcagatc cgcgaaatac caccccatat ccgggtgagc ggcccccacg
     1141 gtatcgaaac cgcatatcga gtggagcccc tcgaaggccg gtccccagta ctccgtacat
     1201 gttgactcat tcaggacgtt gcacgaggcg aggacgatcc agtcggtctt cgtgttgccc
     1261 cagcgcgcct cgctgtagtt caattcataa tcgtcggcgg cagtcccgaa aacgaacccg
     1321 ccctcccatc cgtggccggt aaagaaggcc aggtgcgtgt tgtcgatgta gttcgaatcc
     1381 atcccgccga aagccggatc cttccagtgc cgctccatcg cggcgtcgtc gccgttgatg
     1441 aactggccgg tccatgagct cgaccagtcg agtgtattat agaagccctc ggcctcctcg
     1501 tcggcccagg tgatagggat ctgcatgccg tgatagtccc ccacccactc ggcgcagaag
     1561 gagtacgtcc tgtagtatga acagatcagg tctctctccg ccgtcatgga cacccagtac
     1621 gcctttcccg ggaacatcag gcgctggtcg ctgtaactgc ctgtcccgcc gttaatcata
     1681 ctgacttcgt aatcctgcgt tcccgcatcg tagccctgtg ccgttgccca cgtgctcccg
     1741 agggggagaa gcatgtcccg cgcgcaggcc tggtgcacat cccagaatcc gacaacgttc
     1801 cagccggaat agaggtggtt tgtcactccc gtctgctc
//
LOCUS       DAOQTR010000472         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_83349_length_1833_cov_2.832958, whole genome shotgun sequence.
ACCESSION   DAOQTR010000472 DAOQTR010000000
VERSION     DAOQTR010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83349_length_1833_cov_2.832958"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKN85_10005"
     CDS             complement(<1..376)
                     /locus_tag="PKN85_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNT44788.1"
                     /translation="MKLVANIFREYDIRGNAEEELTSETVRAIGLAYGTFLSREGVRK
                     ITVGGDVRLSTDRIKGDIIEGVTSAGVDVVDIGMVTSPLLYWSLFHLDLDGGVMITGS
                     HNPKEFNGLKLAFGKTTLYGDQI"
     gene            complement(419..1441)
                     /locus_tag="PKN85_10010"
     CDS             complement(419..1441)
                     /locus_tag="PKN85_10010"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HNT44789.1"
                     /translation="MKILILGANGFIGSHLCEHLLDRTDWEITAFDINDFNLGKCMGK
                     ERFSFRKGDFYTDDRWIEGALNECDAVLPLVGIAKPAFYITNPVMTFHLDFEANLKIV
                     RMCVKHGKRVIFPSTSEVYGMSTDTELKEDESPIILGPVCKSRWIYSCSKQMMDRVIA
                     AYGQEEELRYTLFRPFNWIGPRLDTFEDAKEHKARSITQILYDILHERDVSLVGGGRQ
                     RRSFTWIGDGIEGLEAIIRNENGCADGEIFNIGNPRNNASIKELALVMIRVLKSHPKY
                     GPLAEKARLLEIDSRDYYGKGYEDVLDRVPSVAKANRLLGWEPKTPLDEAVSRTIEAT
                     LMENGL"
BASE COUNT          379 a          518 c          462 g          474 t
ORIGIN      
        1 ggatctggtc gccgtagagg gtggtcttgc cgaaggccag cttgaggccg ttgaactcct
       61 tgggattatg gcttccggtg atcattacgc ctccgtcaag gtccaggtgg aagaggctcc
      121 agtagagcag cggagaagtg accatgccga tatcgaccac gtcgactccg gccgaggtta
      181 caccttcgat gatgtcgcct ttgatcctgt ccgttgaaag gcggacatct ccgcccacgg
      241 tgatcttccg gaccccctcg cgcgaaagga acgttccgta ggccaggccg atggccctga
      301 cggtctcgct tgtaagctcc tcctcggcat tgccccttat gtcgtactcc ctgaagatgt
      361 tggcaacaag cttcatcggg actcctccca gttaaaaaga tcgctcttca tcatagcact
      421 acaggccgtt ctccatcagg gtcgcctcga tggtcctgct gacagcctcg tcgaggggcg
      481 tcttcggctc ccagccgaga agcctattgg ccttcgccac cgacggcacc ctgtccagca
      541 catcctcgta acccttgccg tagtagtctc gggagtctat ctccaggagt cttgccttct
      601 cggccagcgg accgtacttc gggtgcgatt tcaggaccct gatcatcaca agtgcaagtt
      661 cctttatgga cgcgttgttc cgggggttgc cgatgttgaa tatttccccg tcggcgcagc
      721 cgttctcatt cctgattatg gcctcaagtc cctcgatccc gtcgccgatc caggtgaagc
      781 ttcgtctctg gcgtccccct cctacgaggg agacgtccct ttcatgaaga atgtcgtaga
      841 ggatctgggt gatcgaacgt gccttgtgtt ctttcgcatc ctcgaaagtg tcgagcctcg
      901 gtccgatcca gttgaaaggt ctgaaaaggg tgtatctgag ttcctcttcc tgcccgtaag
      961 ccgctattac ccggtccatc atctgtttgc tgcagctgta tatccacctg gatttgcaga
     1021 caggacccag gattataggg ctctcgtcct ccttgagctc agtgtcggtg ctcatcccgt
     1081 agacctccga ggtggacggg aagatcaccc gtttgccgtg tttcacgcac atccggacta
     1141 tcttgaggtt tgcctcgaag tcgaggtgga aggtcatgac cgggttggtg atatagaatg
     1201 caggctttgc tattccgacc agagggagca ctgcgtcgca ctcattcaag gccccctcta
     1261 tccatcggtc gtcggtgtaa aagtcgccct tccggaagga gaaccgttcc ttgcccatgc
     1321 acttgccaag gttgaaatcg ttgatgtcga aagccgttat ctcccagtcg gttctgtcca
     1381 gaagatgctc gcacagatga gagcctatga aaccgttggc accgaggata agtatcttca
     1441 caaaaaaccc ccctgtttcc gtaagtcgcg aaccgtagga tcaagagcgt aaacggtaat
     1501 agcaaacttt aaagccaggg ccaaaggtga aacttaaagc cctttcacca cagagaacac
     1561 agagaagagc aggagagagg aaacatagaa cctctttatg gataaaatca gtcaagtccg
     1621 gaacaaaaac cttctatgac tattccgttc attgttttca gtttaagcct tcttggcctt
     1681 ggtcatggtc cctaaaacaa atattgtgat tttctctctt gctcttctct gtgttctctg
     1741 tggtgcaaga tatgggttct gtttttcttc taacgtctca cggatttaaa cttttcctgt
     1801 ctcctgccgc ctggctcttc tcctccaccc tta
//
LOCUS       DAOQTR010000473         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_83986_length_1825_cov_2.622034, whole genome shotgun sequence.
ACCESSION   DAOQTR010000473 DAOQTR010000000
VERSION     DAOQTR010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83986_length_1825_cov_2.622034"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKN85_10015"
     CDS             complement(<1..299)
                     /locus_tag="PKN85_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HNT44790.1"
                     /translation="MFDLNCDVGESFGAWVMGNDAEIIRHVTSANIACGVHASDPSVM
                     RRTVELCRQYGVMAGAHPGYPDLQGFGRRHMDMQGGEVADWVVYQVGALAGFTSRA"
     gene            557..961
                     /locus_tag="PKN85_10020"
     CDS             557..961
                     /locus_tag="PKN85_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44791.1"
                     /translation="MAKKGMETAKRMSRVAASDSDRTRLRRFLFLWAPIIVIVLVGFY
                     VVALDPAKPTGRTIEASVIGKEEAAGGPSSTSMFRVRFDNGEEAVMFIPERQVPPPPG
                     RILAEEYTTSLLSKRTYRYLRAVGATGTPGTP"
     gene            complement(977..1624)
                     /locus_tag="PKN85_10025"
     CDS             complement(977..1624)
                     /locus_tag="PKN85_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44792.1"
                     /translation="MNLLSILAFAVSSSFDNVGVGVAYGMRGVCIPFLSNLVIAAMNG
                     SGTLVSMLAGTSATRFLESGVSSVLGGSIMICTGLWVFIDWMRHPRRPGSIEEDMVER
                     SRSLAGKSFPDMVWAIFRNPFITHPQCSGSMKAVETLLIGSGLTMTNLVTGLAAGMLG
                     VDVAGLTVASFLSNLVAMQLGTSAGASRALRRLGHASGAISGVLLAGIGLAEMVF"
     gene            complement(1621..>1825)
                     /locus_tag="PKN85_10030"
     CDS             complement(1621..>1825)
                     /locus_tag="PKN85_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44793.1"
                     /translation="RLRRGAYSEAGLSRWLEKIGSQDWNRAFVYFKHGEGAGSPGAAM
                     RFDELVRNDRRPAVAGGTPGDGT"
BASE COUNT          381 a          597 c          523 g          324 t
ORIGIN      
        1 gcccgtgagg tgaaacccgc gagtgccccg acctggtaga ccacccaatc cgcgacctcg
       61 ccgccctgca tatccatgtg gcgcctgccg aacccctgga ggtcgggata accggggtgt
      121 gccccggcca tgacaccgta ttgcctgcaa agctccacag tccttctcat cactgacggg
      181 tccgacgcgt gaacaccaca ggcaatgttg gcggaggtca catggcgtat gatctccgca
      241 tcgttcccca tcacccacgc tccgaaagac tcccccacat cgcaattcag atcgaacacg
      301 ccacgcatag ttcccaattc taccagaaaa ccgcctgcaa agacagtccc cgctctcgtt
      361 tgttcggtgc cccgatcgtg ggctgggagc ggtgcctccc gcttcagcgg gaggcaaaac
      421 tggccggatt ttggaaggct ccgaaatccg gccgcatctt ttctctgcga tctccgcggt
      481 ctcaagctaa gcgggcggga ggcagggtct tttcagcctt tacaacactt cctgcttctg
      541 ctaagttttg ggagccatgg cgaaaaaggg tatggaaacg gcaaagcgga tgagcagggt
      601 cgcggcttcc gatagtgaca ggacccggct caggaggttc cttttcctct gggcgcccat
      661 catcgtgatc gtccttgtcg gcttctacgt ggtcgccctc gatccggcaa agccgacggg
      721 caggaccatc gaagcgagcg tcatcgggaa ggaggaggct gccggcggac cgtcatcgac
      781 ctcaatgttc agggtccgtt tcgataatgg ggaggaggcg gtcatgttca tccccgaacg
      841 acaggttcca ccgccgccgg gacggatcct ggcggaggaa tacaccacaa gccttctcag
      901 caaaaggacc tacaggtacc tgcgtgccgt aggcgccacg gggaccccgg gaactccata
      961 gccacgagcc ctcggttcag aagaccatct ccgccaaccc tatgccggca aggagcacac
     1021 cggatatggc accgctcgca tgtcccagtc ggcgtaacgc gcgcgaagcg cccgcgctcg
     1081 tcccgagttg cattgccacg aggttggaga ggaaggaggc gacggtaagg ccggcgacgt
     1141 cgacgcccag catgccggcg gccagacctg tcacaaggtt cgtcatcgtt agacccgagc
     1201 cgatgagaag cgtctcaacc gccttcatcg atccgctgca ttgcgggtgg gtgataaatg
     1261 gattcctgaa gatggcccac accatgtccg gaaagctctt gcccgcgagg gagcgggacc
     1321 tctcgaccat gtcctcttcg atggaacccg gccggcgggg atgacgcatc cagtcgatga
     1381 atacccagag acccgtgcat atcattatgg atcccccgag gacgcttgaa accccggact
     1441 cgaggaaccg ggtcgccgac gtgcccgcaa gcatggaaac gagggtaccc gatccgttca
     1501 tggctgctat tacaaggttt gataagaagg ggatgcagac ccctctcatc ccgtacgcaa
     1561 cacccacacc cacgttatcg aaactgctgg agaccgcgaa cgccaggatg gacagcaggt
     1621 tcatgtccca tcgcccggtg tgccaccggc gactgccggc cgcctgtcgt tgcgcacgag
     1681 ttcatcgaac cgcatcgccg ctcccgggct ccctgcaccc tcgccatgct tgaaatagac
     1741 aaacgccctg ttccaatcct gggagcctat cttctcaagc caccgcgaca acccagcctc
     1801 actgtaggcg ccgcggcgaa gacgc
//
LOCUS       DAOQTR010000474         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_84029_length_1824_cov_3.655172, whole genome shotgun sequence.
ACCESSION   DAOQTR010000474 DAOQTR010000000
VERSION     DAOQTR010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84029_length_1824_cov_3.655172"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..551)
                     /locus_tag="PKN85_10035"
     CDS             complement(57..551)
                     /locus_tag="PKN85_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNT44794.1"
                     /translation="MSKTQKNLEEAFAGESQANRKYLAFAKKADEEGFKQVAKLFRAA
                     AEAETIHAHNHLRELKGIKSTKENLMEAVGGETHEFKSMYPAMIEEAKAEGNTGAVRT
                     FQFANDVEIVHAGLYQKALDSLGNNEDTDYWVCKVCGNTVEGEPPDECPVCKAKKVAF
                     YKVA"
     gene            complement(585..1808)
                     /locus_tag="PKN85_10040"
     CDS             complement(585..1808)
                     /locus_tag="PKN85_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNT44795.1"
                     /translation="MKPIEIKPGIYWVGVIDWAIRDFHGYVTKNGTTYNNYLVMDDEV
                     TLLDTVKHDFDATAVENIRRVVDPYKIKNIVINHIEPDHASSIAAIMEYTPDATIFIT
                     ERGKKGLDRHFDTSRWKFRIVKSGDTLKTGKYTLTFLETPMLHWPDSMVTYIPEAKLL
                     ISQDAFGQHMASAARFDDEFFSCHSQFELDDAIVDYYANILMPFGKLIEAKIQEIVRL
                     GLEIDMIAPDHGIIWRKDPGYIIQRYLDLAKGKADLAAVIIYDTMWHSTELMSDPIMN
                     GIKDEGVPCKVIKLRSTSMSEAIKEFWRARGCLIGTPTINNVMFPSVAEFLYHLAGLR
                     PKDRIVGGFGSYGWGGGAVKEAYAKFQQMGLEAVEPGLQVLYKPSAEDMDRCYEFGRD
                     FAKRLKEYHGKFQAV"
BASE COUNT          331 a          565 c          468 g          460 t
ORIGIN      
        1 ccatgaaaag ggcgagaggg ggggccgcct cacccctgcc ctcccggata ccgatatcag
       61 gccactttgt agaaggccac cttcttcgcc ttgcagaccg gacactcgtc gggcggctcc
      121 ccctccacgg tgttgccgca caccttgcag acccagtagt ccgtgtcctc gttgttgccg
      181 aggctgtcga gcgctttctg atagagaccg gcgtgcacga tctcaacatc gttggcgaac
      241 tggaaggtcc tcacggcgcc cgtgtttccc tccgccttcg cctcttcgat catcgccggg
      301 tacatggatt tgaactcgtg ggtctcacca ccgaccgcct ccatgaggtt ttctttcgtg
      361 ctctttatgc ccttcagttc cctcagatga ttgtgggcgt ggatggtctc cgcctccgcc
      421 gccgccctga aaagctttgc cacctgtttg aaaccttctt catccgcctt cttcgcgaag
      481 gcgagatact tgcggttggc ctgcgattcg ccggcaaatg cttcctcgag attcttctgc
      541 gtctttgaca tggtccctcc ttctgatatt attcacacct atgatcagac cgcctggaac
      601 ttcccgtggt actccttcaa acgcttcgca aagtcccggc cgaactcgta gcacctgtcc
      661 atatcctcag cggacggttt gtacagcacc tggaggcccg gttccaccgc ttcgagaccc
      721 atctgctgaa atttggcgta ggcctccttg acggctccgc ctccccatcc ataactgccg
      781 aacccgccaa cgatcctgtc cttcggtctc aaacccgcca ggtgatacag gaactccgct
      841 acggatggga acatgacatt gttgatggtc ggggtcccga tcaggcagcc ccgcgccctc
      901 cagaactcct ttatcgcctc gctcatggat gtcgacctca gtttgatgac cttgcagggc
      961 accccttcgt ccttgatgcc gttcatgatg gggtcgctca tgagttccgt cgaatgccac
     1021 atcgtatcgt agatgatgac ggccgcgagg tctgccttgc ccttcgccag atccaggtac
     1081 cgctgtatga tgtaaccggg gtccttcctc catatgatcc cgtgatcggg cgctatcatg
     1141 tcgatctcga gcccgagcct tacgatctcc tggatcttcg cctcgataag ctttccgaaa
     1201 ggcatgagga tgttagcgta ataatccacg atggcatcgt cgagctcgaa ctgggagtgg
     1261 caggaaaaga actcgtcgtc gaacctggcg gccgaggcca tgtgctgacc gaaggcgtcc
     1321 tgcgatatga ggagtttcgc ttcgggtatg taggtcacca tggaatccgg ccagtgaagc
     1381 atgggcgtct ccaggaacgt cagcgtgtac ttccccgtct tcagggtatc gccgcttttg
     1441 actatcctga atttccagcg cgacgtgtcg aaatggcggt ccagaccctt ctttcccctc
     1501 tccgtgatga agatggtcgc gtcgggggta tactccatga tcgccgcgat gctgctggcg
     1561 tggtccggtt cgatgtgatt gatcacgatg ttctttatct tgtacggatc caccacgcgc
     1621 cgtatgttct caacggccgt ggcgtcgaaa tcatgcttga cggtatcgag aagggtcacc
     1681 tcgtcatcca tgacaagata gttattgtat gtggtgccgt tcttcgtgac atacccatga
     1741 aaatcccgga tcgcccaatc gatcacccca acccagtata tacccggttt gatctctatc
     1801 ggcttcattt atcctccttt tcgg
//
LOCUS       DAOQTR010000475         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_84584_length_1817_cov_3.272985, whole genome shotgun sequence.
ACCESSION   DAOQTR010000475 DAOQTR010000000
VERSION     DAOQTR010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84584_length_1817_cov_3.272985"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKN85_10045"
     CDS             <1..657
                     /locus_tag="PKN85_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT44796.1"
                     /translation="FVSIRAKGIYFAILTLAFAEVVYRIIFHSYNTPLGGSDGLVGVP
                     TPMLHLGFFDIDLRNTVNFYWVAVAFAYLSYLVCKLIVGSPFGSILSGIRENENRVPF
                     LGFNVKWSKVTAFVLAGIFAGFSGAMFTAFKTYADTEQLNFLLSGKVIVMALIGGIGT
                     LIGPMVGAVVITIFESVISTYFRAHHLIIGALFVIVVIFMPKGLLGLFARKDRNNNGS
                     "
     gene            660..1397
                     /locus_tag="PKN85_10050"
     CDS             660..1397
                     /locus_tag="PKN85_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT44797.1"
                     /translation="MFFEVKELCRSFGALQAVHNVSFTLDEGTTLSIIGPNGAGKTTL
                     FNVITGVFPPDSGEVMFKGTQVTGMPPDKLCHKGIARSFQITNIFQGLSVFENIRLAC
                     QGRKATKRMFSHVSKLKEPVEEAEKILDLLGLLDLRDSRAGTLSHGDQRYLEIGMTLA
                     AKPSLVLFDEPTAGMTPMETKATIELIHKLKGMVTIIIIEHDINMVFAVSDRILVMDQ
                     GNVLADGPPEEVKANEAVKAAYFGEEI"
     gene            1397..>1817
                     /locus_tag="PKN85_10055"
     CDS             1397..>1817
                     /locus_tag="PKN85_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT44798.1"
                     /translation="MLEVKDLNTFYGKSHILQGINLDVREGEIVCLLGRNGVGKSTTL
                     KSIMGLVTPSRGTVTFEGREIQKMRPDVIARMRISYIPEDRRIFSRLSVKENLLIGTS
                     NAPDMTKARKEEMLEKAYLYFPILKEKESQGGGYLSGG"
BASE COUNT          406 a          487 c          527 g          397 t
ORIGIN      
        1 ttcgtatcga tccgtgcgaa gggcatatac tttgccatcc tgaccctcgc ctttgccgag
       61 gttgtctaca ggatcatctt ccattcctac aacacccctc tgggcggctc cgacgggctc
      121 gtcggcgtgc ccacgccgat gctgcacctc gggtttttcg acattgacct gaggaacacg
      181 gtcaacttct attgggtggc ggtcgctttt gcgtacctct cctacctggt ctgcaagctg
      241 atcgtcggtt cccccttcgg cagtatcctt tcgggtatac gggaaaacga gaaccgcgta
      301 cccttcctcg gtttcaatgt gaagtggagc aaggtgacgg cctttgtcct ggcgggtatc
      361 ttcgcagggt tcagcggggc gatgttcaca gccttcaaga cctacgcgga tacggagcag
      421 cttaacttcc tgctctccgg caaggtcatc gtcatggccc tcatcggcgg cataggcacg
      481 cttatcggcc ccatggtggg agcggtggtg atcacgatct tcgaatccgt catctccacg
      541 tattttcgtg cccatcacct tattataggg gcgctctttg tcatcgtagt catattcatg
      601 ccgaaagggc ttctcgggtt gttcgcgaga aaagacagaa ataataacgg gagttgataa
      661 tgttctttga ggtcaaggag ctgtgcaggt ctttcggggc gcttcaggcg gttcacaatg
      721 tttccttcac gctggacgag ggtaccaccc tttccatcat cggaccgaac ggggccggga
      781 agacgaccct tttcaacgtg atcaccggtg tgtttccgcc cgatagcggt gaagtgatgt
      841 tcaagggcac gcaggtgacg gggatgcccc cggacaagct gtgtcacaaa ggtattgcga
      901 ggtcgttcca gataacgaat atctttcagg ggctctccgt gttcgagaac atccgtctcg
      961 cgtgccaggg gcggaaagcg acgaaaagga tgttttccca cgtctcaaag ctgaaggagc
     1021 ccgtggaaga ggcggagaag atactcgatc tgctcgggct tctcgacctt cgtgacagta
     1081 gggccggtac tctttcccac ggtgaccaga gatacctgga gatcggcatg accctcgcgg
     1141 ccaaaccgtc gctcgttctc ttcgacgaac cgaccgcggg catgacaccg atggagacga
     1201 aggccacgat cgaactgatc cacaagctga agggaatggt cacgataatc atcatcgagc
     1261 atgatatcaa tatggtcttc gctgtctccg acaggatcct cgttatggac cagggaaacg
     1321 tcctggcgga tggtcctccc gaggaagtca aggcgaacga ggccgttaag gccgcgtatt
     1381 tcggggagga gatataatgc tcgaggtcaa ggatctgaac acattctacg gaaaaagcca
     1441 tatactccag ggtatcaacc tcgatgtcag ggagggcgag atcgtatgcc ttctcggacg
     1501 gaacggggtc gggaagagta ccacgttgaa gtcgatcatg ggactcgtca cccccagccg
     1561 gggcaccgtg accttcgaag ggagagagat acagaagatg agacctgacg tcatagcacg
     1621 gatgagaata agctacatcc ccgaggacag aaggatcttc tcccggcttt cggtgaagga
     1681 aaacctcctg ataggcacca gcaacgctcc cgacatgaca aaggcccgga aggaggagat
     1741 gctggagaag gcgtacctgt atttccccat cctgaaagag aaggaatccc agggcggagg
     1801 gtacctttcc gggggcc
//
LOCUS       DAOQTR010000476         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_84837_length_1814_cov_2.700966, whole genome shotgun sequence.
ACCESSION   DAOQTR010000476 DAOQTR010000000
VERSION     DAOQTR010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84837_length_1814_cov_2.700966"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..885
                     /locus_tag="PKN85_10060"
     CDS             103..885
                     /locus_tag="PKN85_10060"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNT44799.1"
                     /translation="MLLVIDIGNTNIVFGVYHDGKLLNHWRLSTALGKTVDEYAILLN
                     SLLYIEKIRLNEIHSAIVSCVVPPLLIPFEIVCRKYIGIMPIVVEPGIKTGMPVLYEN
                     ATELGADRIVNAVAGYDKYKKALIIVDFGTATTFEYITPKGEYAGGSIAPGILISLDA
                     LFERASKLPRVELVQPKTLISKNTVQAMQAGITYGYVSLVDGIVKRMKEEVRTDPLVI
                     ATGGLSSLIYKGSETIDVVDEFLTLEGLKILYEKNKDHHKFK"
     gene            complement(1058..>1814)
                     /locus_tag="PKN85_10065"
     CDS             complement(1058..>1814)
                     /locus_tag="PKN85_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNT44800.1"
                     /translation="KVSRETGLKTRGAQTMNYVLAVLAVGFIVLIHETGHFIAAKLAG
                     IPIRTFSVGFGPRLYALKKGGTEYRLSLIPLGGYVMPDIDDEKAFFDLPVLRRVVLAA
                     GGPAASMVLPLFCFALSDALRFGPGPGSLLFQPLEQTATAFIRIASIIPLLFTHHGEL
                     SGIAGIVSQGGRFIGTDGHNLLSFTALMSINLAVLNLLPVPVLDGGKIVMYAMEKLSR
                     KVVKLHYPLSVAGWALMLAIMIYATVLDVGRMM"
BASE COUNT          436 a          512 c          513 g          353 t
ORIGIN      
        1 ggtctacgcc gggacggcga aaggcatcga ccgggttggt gcgatccttc tcgacataga
       61 gggcaccacg aagaaaatcc ttgcaggaga tgtgaacttc taatgctact tgtcatcgat
      121 atcgggaata ccaacatcgt ttttggcgtc tatcacgacg gcaagctgct caaccactgg
      181 aggctgtcca cggccctggg caagacggtc gacgaatacg ccatcctcct caacagcctt
      241 ctctacatag agaagatacg cctgaacgag atacacagcg ccatcgtgtc ctgtgttgtg
      301 ccgccgctcc tgatcccctt tgagatcgtg tgcaggaaat acatcggcat catgcccatc
      361 gttgtcgagc ccggcatcaa gacgggaatg cccgtacttt acgagaacgc cacggaactc
      421 ggtgccgaca ggatcgtcaa cgccgtggcc gggtatgaca agtacaagaa ggcgctcatc
      481 atagtcgatt tcggcaccgc caccaccttc gaatacatca cccccaaagg ggagtacgcg
      541 gggggttcca tcgccccggg tatcctcatc tccctcgacg ccctcttcga gcgggcatcc
      601 aaacttccgc gcgtcgagct cgtccagccg aagacgctca tcagcaagaa caccgtccag
      661 gcgatgcagg ccggcatcac ctacggctat gtgagcctcg tcgacgggat cgtcaagagg
      721 atgaaggaag aggtcaggac cgatcccctg gtcatcgcga cgggcggtct ctcaagccta
      781 atctacaagg gttccgaaac gatcgacgtc gtcgacgagt tcctcacgct ggagggactg
      841 aagatcctct acgagaagaa caaggaccat cacaagttca agtaaataaa gaaccgtttt
      901 aggttttaag ttttaagtaa aaagaaaagg cctcacgggg gccttcctgc gtttcactca
      961 ctatcttccc ttgccggaga cattgcctcc ggcactgtct ttgacttaaa acttaagact
     1021 taaaacctaa aacttaaaac ttaaaacggt ccttcactta catcatcctc cccacatcca
     1081 gcacggtcgc gtagatcatg atggcgagca tgagggccca gccggctacg gagagggggt
     1141 agtggagctt gacgaccttc cggctcagct tctccatggc gtacatgacg atctttccgc
     1201 cgtcgaggac gggcacggga aggagattga ggacggcgag gttgatgctc atgagggccg
     1261 tgaaggacag gaggttgtga ccgtccgtcc cgatgaagcg gccgccctgg gatacgatcc
     1321 cggcaattcc cgaaagctcc ccatggtggg taaaaaggag gggaatgatg gaggcgatcc
     1381 tgatgaaggc ggtggccgtc tgttccaacg gctgaaaaag aaggcttccg ggaccgggtc
     1441 cgaaacgcag cgcgtctgac agcgcgaaac agaacaaggg caggaccatg ctggccgcgg
     1501 ggccgccggc ggcgaggact acccgtcgaa ggacaggcag gtcgaagaac gccttctcgt
     1561 cgtcgatgtc cggcatgaca tatccgccga ggggaatgag ggacagtctg tattccgtac
     1621 cgcctttctt cagcgcatag agtctcgggc cgaagccgac ggaaaaggtc cggataggta
     1681 tccctgccag ctttgcggcg ataaaatggc ctgtttcatg gatgagaacg ataaacccga
     1741 cagcaagcac ggcaagcacg tagttcattg tctgcgctcc tcttgtctta agaccggtct
     1801 cccgggagac cttt
//
LOCUS       DAOQTR010000477         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_84904_length_1813_cov_2.910694, whole genome shotgun sequence.
ACCESSION   DAOQTR010000477 DAOQTR010000000
VERSION     DAOQTR010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84904_length_1813_cov_2.910694"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PKN85_10070"
     CDS             <1..439
                     /locus_tag="PKN85_10070"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HNT44801.1"
                     /translation="ALGEWLKRRFRSDSSTFVQGFVTASILYCTGAMVIVGSIRDGTV
                     GDPSILYIKSLLDGVASVAFASSLGLGVAFSALSVLVIQGSITLLASQLMFLQEPAVI
                     EAVTATGGLLILGIGINILDIKQIRVGNLVPALAYAVAAAMYF"
     gene            complement(573..989)
                     /locus_tag="PKN85_10075"
     CDS             complement(573..989)
                     /locus_tag="PKN85_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNT44802.1"
                     /translation="MATVKMKRYKSMLNHRIEEILKANGKPLFPAENLNGLLPDMYDL
                     ASFESERLYEYALKDRERKTVLELIEAIKRIEEGNYGICEDCLKPISEKRLQVAPAAT
                     LCIECKAEEEKRQRAENIRRVHAHHGIQLGEPPGGN"
     gene            complement(1335..>1813)
                     /locus_tag="PKN85_10080"
     CDS             complement(1335..>1813)
                     /locus_tag="PKN85_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44803.1"
                     /translation="TRVNVGTLQAGTTWNVIPDMATFRMETRGVTNELNEYMVQKARE
                     VIEGAAKMYDLAVEMKPAAMAVAAESSPELIATAERVAGTLPSVKKVVPSAPFNASED
                     ITLMMERVQQKGGKALITLFGTPTHGGHHNSTFDADEIVIRNAAEFYAAMQREIAG"
BASE COUNT          332 a          539 c          525 g          417 t
ORIGIN      
        1 agccctgggt gagtggctca agaggcgctt ccggtccgac tcgtccacct tcgtccaggg
       61 gtttgtcacg gcctccatcc tctactgcac gggggccatg gtcatcgtcg gctcgatccg
      121 cgacgggacc gtgggcgacc catccatcct gtacatcaag tcccttctcg acggcgttgc
      181 atccgtcgcc tttgcctctt cgctcgggct gggcgtggcc ttctccgcgc tgtcggtcct
      241 cgtcatccag ggttccatca cgctgctggc atcgcagctc atgttcctcc aggagccggc
      301 cgtgatcgag gccgtgaccg ccacgggggg gctcctcatc ctcgggatcg gcatcaacat
      361 cctcgatatc aagcagatcc gcgtgggcaa tctcgtcccc gcgctggcct atgccgtcgc
      421 ggcggcgatg tacttctagg gtccaggatc cagggttcag ggaacagggt aggtggaaac
      481 aggaaggccc ccgttcgggg gccttcctgt ccagatgaaa ggagtaaaga gttgggtgtc
      541 tctcagcgac ccgcatggag gggcgcgttc tctcaattgc cgcccggggg ctcgcccagc
      601 tgtatgccgt gatgggcgtg aaccctgcgt atgttctccg cgcgctgccg cttttcctcc
      661 tcggccttgc actcgatgca cagcgttgcc gcgggtgcga cctgaagcct cttttcggag
      721 atgggtttca ggcagtcctc gcagatgccg taattaccct cctcgatccg cttgatggcc
      781 tcaatcagct cgaggaccgt ttttctctcc cggtctttca gtgcgtactc atagagacgc
      841 tccgattcga acgaggccag gtcgtacatg tcggggagga gcccgttgag attttccgcg
      901 gggaacagcg gctttccgtt ggccttcaga atctcttcaa tccggtgatt caacatcgat
      961 ttgtaacgct tcattttcac cgttgccatc gttcaacctc cttttccaga agtacgactt
     1021 agcaacaggc ctgccaagtg tccgacgccg cgaaattaat tcgtaagttg ctgtcatgaa
     1081 agaagaaaat ggcgatccac cccatagcgc gaagagaaac gggaatgcgc ttttccgcac
     1141 aaaaacggaa aaagccgtcg caggagggaa actgttgact tttaagttat tgaataaatg
     1201 agagaattat taaagcgaca tttttgtcgc aaaagaggac cgaaaggtcg tcaaggacag
     1261 acaagaccgg caaaatatgt cgaatgatcc cgtgtgccat ccatcctgcg caccgcgggt
     1321 cgggcttgcg gtgcttatcc tgcaatttcc ctctgcatgg cggcgtagaa ttccgccgcg
     1381 ttgcggatca cgatctcgtc ggcgtcgaag gttgagttgt ggtgcccgcc atgggtcgga
     1441 gtcccgaaca gggtgatgag ggccttgccc cctttctgct ggacgcgctc catcatcagg
     1501 gtgatgtctt ccgaggcatt gaagggggcg ctcggcacga ccttcttcac ggagggaagt
     1561 gttccggcca cccgctccgc cgtggcaatc agctcaggcg aactctcggc cgccaccgcc
     1621 atggctgccg gtttcatctc gacggcaaga tcgtacattt ttgcagcccc ttcaatcacc
     1681 tcgcgggcct tctggaccat gtactcattg agttcgttgg tcacgccgcg ggtctccatg
     1741 cggaaggttg ccatgtcggg gatgacgttc catgtggttc cagcctggag ggtgcctaca
     1801 ttaacccgcg tag
//
LOCUS       DAOQTR010000478         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_85222_length_1809_cov_3.571266, whole genome shotgun sequence.
ACCESSION   DAOQTR010000478 DAOQTR010000000
VERSION     DAOQTR010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85222_length_1809_cov_3.571266"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKN85_10085"
     CDS             complement(<1..509)
                     /locus_tag="PKN85_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNT44804.1"
                     /translation="MESLYTLIEMVVKNIVVVLVLMGCVAYTTLLERKLLGRLQVRLG
                     PNRVGPFGLLQPIADGVKSFFKEDIIPDEADKTLYVLAPGISLFAALSMFAVIPFGDT
                     VTFLGRQIKLVVADLDTGLLYLLALATLGEYGVVLGGWASGNKYGVLGALRAAAQMIL
                     DTSRRADHDV"
     gene            complement(513..>1809)
                     /locus_tag="PKN85_10090"
     CDS             complement(513..>1809)
                     /locus_tag="PKN85_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNT44805.1"
                     /translation="DLVVVAGADLLGNNHLLANKVREVVKNKGTKVAVVDPSSAALTR
                     ACDVWVKVAPGSDALFFNGVASQLIADKKNDPEAGSLEGFSGSTGGGKGDAVRSGADE
                     KSVDRFYALFAAASRVAVIFGTGISDRTESMEALLNLCLVKGVQKTGVVMPTALQSNA
                     VGVISVLPDAVSSGDVLGEADGLFIYEDDPFHYLGGKRVEESLKNKAFVAVCDMFPTG
                     ASALAHVVVPSGSFAQKDGSYVAEDGYVRDVARAEGGSSPGFDYLRLLLDRLGGGLYR
                     DEQEASRVLFGKEVLVADDSGRAMLKPTNGQARLASAGKAAEGKPARPLTLVLRNVFF
                     HHHLAGQGVYSKMVYLQNPAVAGDKLFISPEDAAALGIADGGQVIVESDHGTLQHPVT
                     VKEGLGKGVLEYRMLKNRQDVLKLADGYGKHIAVTVKKG"
BASE COUNT          360 a          592 c          508 g          349 t
ORIGIN      
        1 acgtcgtggt cggcacgccg ggacgtatcc aggatcatct gcgccgcggc acgcaatgcc
       61 ccgagcaccc cgtacttgtt ccctgaggcc cacccgccga gaaccacacc atactcaccg
      121 agggtcgcga gggcaagcag gtacagaagg cccgtatcca ggtcggcaac gaccagcttg
      181 atctgtctgc ccaggaaggt tacggtgtct ccgaagggaa tgaccgcgaa catggagagc
      241 gccgcgaaga gggagatgcc cggtgcgagg acgtagagag tcttgtccgc ctcgtcaggt
      301 atgatgtctt ccttgaagaa agacttgacg ccgtccgcga tcggctgaag gagaccgaag
      361 ggccccaccc tgttgggacc gagcctgacc tgcagacggc cgagcagttt tcgctcaagg
      421 agcgtggtat aggccacaca tcccatgagg acgagcacca caacgatgtt cttgaccacc
      481 atttcaatca aagtatagag cgattccatg ccttaacctt tttttaccgt cactgcgatg
      541 tgtttgccgt atccgtcggc aagcttcaat acgtcctgcc tgttcttcag catcctgtat
      601 tcaagcacgc ccttgccaag accttccttg accgtgacgg ggtgctgcag tgtcccgtgg
      661 tccgattcga cgatgacctg accgccgtcg gctataccca acgccgcggc gtcttccggt
      721 gaaatgaaca gcttgtctcc ggcaaccgcg ggattctgga ggtacaccat cttcgagtag
      781 acaccctgtc ccgccaggtg atggtggaag aagacattcc tcaggacaag ggtgagcggt
      841 ctcgccggct tgccctccgc ggctttgccc gctgaggcca gacgtgcctg gccgttcgtg
      901 ggtttgagca tcgccctgcc gctgtcgtcc gccacgagga cctccttccc gaagagaacc
      961 ctggatgcct cctgttcgtc cctgtacagg ccgccgccca accggtcgag gaggagcctc
     1021 aggtagtcga accccggcga ggatccgcct tccgcccgtg ccacatcgcg cacgtaacca
     1081 tcttccgcga cgtagctgcc gtccttctgg gcaaagctgc cggaggggac gacgacatgc
     1141 gccagcgcgg aggcgcccgt cgggaacatg tcacagaccg ccacaaaggc cttgttcttc
     1201 aatgattctt ccaccctctt tcctccgagg taatggaaag gatcgtcctc atagatgaag
     1261 aggccatccg cctcacccag aacatccccg gaagagacgg catcgggcag gaccgagatg
     1321 actccgacgg cattggactg gagcgccgtc ggcatcacga cccccgtctt ttgaacgcct
     1381 ttcacgagac agaggttgag gagcgcttcc atgctctccg tcctgtcgct gataccggtg
     1441 ccgaatatga cagccacgcg ggaggccgcg gcgaaaagag catagaacct gtccacgctc
     1501 ttctcgtcgg ccccgctccg tactgcatcc cctttgcctc caccggtact acccgaaaaa
     1561 ccttccagac ttcccgcctc cgggtcgttc ttcttgtccg cgatcaactg cgaggcgacg
     1621 ccgttgaaga acagcgcgtc ggaacccgga gcgaccttca cccacacgtc acaggctctc
     1681 gtcagtgccg cggaggaagg gtcgacaacg gccaccttcg ttcccttgtt cttcaccact
     1741 tcccgcactt tgttcgcaag gagatggtta ttgccgagaa ggtcggcgcc ggcaacgacc
     1801 acgagatcg
//
LOCUS       DAOQTR010000479         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_86136_length_1797_cov_3.901263, whole genome shotgun sequence.
ACCESSION   DAOQTR010000479 DAOQTR010000000
VERSION     DAOQTR010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86136_length_1797_cov_3.901263"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..534)
                     /locus_tag="PKN85_10095"
     CDS             complement(85..534)
                     /locus_tag="PKN85_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44806.1"
                     /translation="MNTSLITAQNLRTGIFAPTIREMQRGESQRREDREGAASHKGLP
                     SRFVPFESDDVTVSSGRKPAPGGAREQLPVHVPASGSPQTMEERRDGQDGVLTVKGPG
                     SAVESGVIRYTVNVGPYDTSVSTVAMRKYGEASSHRAWRPGTFEVTI"
     gene            697..1494
                     /gene="nudC"
                     /locus_tag="PKN85_10100"
     CDS             697..1494
                     /gene="nudC"
                     /locus_tag="PKN85_10100"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNT44807.1"
                     /translation="MNDDVSSHPVFLMRDHKLLVDLSSGVPRVPDRSVAEEFGLSFAD
                     GYRLGPLNGVDCYALSVTGEDEPSRGMDYLGLRKLFGVLDDTRYDMAVKALAILNWDR
                     TCRFCSACGSPLRRHETILAKVCEVCGSTMFPRISPAVIVLVERHEKVLLARASRFAE
                     NLYSVLAGFTEPGETLEDTVRREVREEVGVEVGDIRYFGSQPWPYPDSLMIGFTASWV
                     AGEIAADNDEITDARWFTADDLPKIPDRLSISRALIDWFIDKHRKGK"
     gene            1496..>1797
                     /locus_tag="PKN85_10105"
     CDS             1496..>1797
                     /locus_tag="PKN85_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNT44808.1"
                     /translation="MQAIVFDGRLKLTGGRPRPVPARGEALIRVTMAGICNTDMEITK
                     GYLGFRGIMGHEFTGVVEAEGGRSGRLVGKRVVGEINCGCGACDLCLAGLEKHCPQ"
BASE COUNT          343 a          523 c          546 g          385 t
ORIGIN      
        1 tgtcgctgcc tcccattcgg cactggtcaa ctcggcgaat tcgctattct tttatccctg
       61 ggatgcggtc ggcgctattg ccgttcagat tgtcacttca aaggtaccgg ggcgccaggc
      121 gcggtgggag cttgcctctc catattttct catcgccacc gtgctcacac tggtatcgta
      181 aggaccgaca tttaccgtgt aacggatgac gccgctctca acggcggagc cgggaccttt
      241 caccgtcagc accccgtcct gtccatccct tctctcttcc atggtctgag ggcttcccga
      301 ggcagggacg tggacgggca gctgctcccg cgctcccccg ggagcgggtt tcctgccgga
      361 actgaccgtc acatcatcgc tctcgaaggg aacaaagcgt gagggcaatc ccttgtgtga
      421 agccgcgcct tcccggtcct cccggcgctg gctctcgcct ctttgcatct cccggattgt
      481 cggtgcaaag atgccggttc gcagattctg ggcggttatg agagaagtat tcatttgaat
      541 gtgttggtat catatcattt ttttcttttt tcgacgagaa aaaaatcgaa ggtccggtac
      601 aacggcctct agagccttgc caggccttct tcttgacttt cattgtctca tgcagtattg
      661 attcatacac tggcaaacga aggagatgaa ccggccatga acgatgatgt cagctcgcac
      721 cccgtcttcc tcatgcgcga ccacaagctg cttgtcgacc tgtcatcggg cgtgccccgg
      781 gtacccgacc ggtctgtcgc tgaggaattc ggattgtcct ttgcggacgg ataccgactc
      841 ggtccgctca atggcgtcga ttgctatgct ctgtccgtta caggcgaaga cgaaccctcc
      901 cggggcatgg actatctggg cctcagaaag ctcttcggcg tcctcgacga caccaggtat
      961 gacatggccg tcaaggccct cgccatactc aattgggaca ggacatgccg gttctgcagc
     1021 gcctgcggtt ccccgctccg acggcatgag accattctcg ccaaggtgtg cgaggtctgc
     1081 ggttcaacga tgttccccag gatatccccg gcggtgatcg ttctcgtgga aaggcacgag
     1141 aaggtcctcc tcgccagggc gagcagattt gccgaaaacc tctacagcgt tctcgcgggt
     1201 tttacggagc ccggggaaac actggaggac acggtgcgca gggaggtgag ggaagaggtg
     1261 ggcgtggagg tgggcgacat ccgctatttc ggcagtcagc cgtggcccta ccccgattcc
     1321 ctcatgatag gcttcacggc ctcctgggtt gccggggaga tcgccgcgga caatgatgag
     1381 ataaccgacg cgcggtggtt caccgcggac gaccttccga aaataccgga caggctgagc
     1441 atctcacgag cgctgataga ctggttcatc gacaaacaca ggaaggggaa gtaggatgca
     1501 ggcgatagtc ttcgacggac gcctgaagct gactggcggc aggcccaggc cggtccccgc
     1561 acgcggtgag gctctgatca gggtcacaat ggcgggcatc tgcaatacgg acatggagat
     1621 aacgaagggg tatctcgggt ttcgcgggat catgggccac gagttcaccg gcgtcgtcga
     1681 ggctgagggc ggcaggtcgg ggaggctcgt cgggaagcgc gttgtcggag agataaactg
     1741 cggctgcggc gcgtgcgacc tttgcctcgc aggccttgaa aaacactgcc cccagag
//
LOCUS       DAOQTR010000480         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_86601_length_1791_cov_3.487327, whole genome shotgun sequence.
ACCESSION   DAOQTR010000480 DAOQTR010000000
VERSION     DAOQTR010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86601_length_1791_cov_3.487327"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKN85_10110"
     CDS             complement(<1..264)
                     /locus_tag="PKN85_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44809.1"
                     /translation="MSRDDEDCVVWAKAGGYVVAFVVVIGAWVPFLPVNLALAVAMRK
                     AKRLFPFALALLVTAKTGLAVFAASWLIHKIGTTPTWLMFLIPG"
     gene            complement(339..1247)
                     /locus_tag="PKN85_10115"
     CDS             complement(339..1247)
                     /locus_tag="PKN85_10115"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNT44810.1"
                     /translation="MAENEKPLSPSHGCAVKVLHAAFEILRERDGETSGRDVIEEIAR
                     RVQFTRWEEGVYEKTGNVRWQGILHFYTIPAVKAGFLIKKKGIWYLTPEGERALEYGP
                     QGLLDAATRAYKEWKREQKTQSEEEPDLINEAGDSQAAEATIDEIEQKAMEGLMASIR
                     KKNAYEFQDLVGALLRGMGYYTPFIAPKGKDGGIDIIAYKDPLGTESPRMKVQIKHMQ
                     IPVSVKEIRELMGLLQKDGDIGIFVSSGGFTSDAKATARTAPVHLELVDLPRFISLWQ
                     EFYPKMVDEDKNLLPLLPVYFLAPTD"
     gene            complement(1297..1713)
                     /locus_tag="PKN85_10120"
     CDS             complement(1297..1713)
                     /locus_tag="PKN85_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNT44811.1"
                     /translation="MTRDEILQFINENPIFALGTIDGDRPRVRYMMVSFADDRGIIFC
                     TGKTKEVYLQLCNVPAVEMCFADADRQRQVRIEATAEETDDLEVKKAIVEKFDFLRPW
                     VDEVGYEGLAAFRLADARACAWTMETNHEAKEHIDL"
BASE COUNT          340 a          567 c          471 g          413 t
ORIGIN      
        1 gccggggatc aggaacatca gccacgtcgg cgtggtgccg attttgtgga tgagccacga
       61 ggcggcgaaa accgccaggc ccgtcttggc cgtcaccagc agcgccaagg cgaacggaaa
      121 gagccgcttg gccttcctca tcgccaccgc cagcgccagg ttcaccggca aaaacggcac
      181 ccacgccccg atgacgacca cgaacgcaac gacatatccg ccagccttgg cccacacaac
      241 acagtcctcg tcatccctgc tcaatacggt tcttctcacg accgaaaccc acgcagcgcc
      301 tccaacctcc ttgccccgag ggccgattac caagccgttc agtcagtagg tgccaggaag
      361 taaaccggca gcaacgggag tagattcttg tcctcgtcga ccatctttgg gtaaaactct
      421 tgccacaatg agatgaatct cgggagatcc acaagttcca gatgtacggg ggccgtccgg
      481 gctgtcgctt tggcatcgga agtaaagccc ccgcttgaaa cgaaaatgcc gatgtctccg
      541 tccttctgaa gcaaacccat gagctcccgg atttccttta cagacaccgg aatctgcatg
      601 tgcttaatct gcactttcat ccggggcgac tcggttccga gcgggtcctt gtaggcaatg
      661 atgtcaatac ccccatcttt tcctttggga gcaatgaagg gcgtgtagta ccccatgcca
      721 cgcaggaggg ccccgaccag atcttgaaat tcgtatgcat tcttcttacg gatgctggcc
      781 atcagcccct ccatggcctt ctgttcgatc tcatcgattg tcgcctctgc cgcttggctg
      841 tcaccggcct cgttgataag gtcaggctcc tcttcgcttt gtgttttctg ttctcttttc
      901 cactccttat atgctcgggt agccgcgtcc aggagtccct gcggaccgta ttcaagagca
      961 cgttctcctt ccggggttaa ataccagatg ccctttttct tgattaggaa gcctgctttg
     1021 actgcgggaa tggtgtagaa atgaaggatg ccttgccaac gcacgtttcc cgtcttctca
     1081 tagacccctt cctcccaacg agtgaattgc actcgtcggg caatctcctc aattacatcc
     1141 ctgcccgatg tttctccatc tcgttcccta agaatctcaa aggctgcgtg aaggactttg
     1201 actgcacagc catgagatgg cgaaaggggt ttttcattct cagccatgtc ggagcccctt
     1261 ctactatgat tgatcccgca ttcactactg cgggtcttac aggtcgatat gttccttggc
     1321 ctcgtggttg gtctccatcg tccaggcgca ggcccgggcg tcggccaggc gaaacgcggc
     1381 caggccctcg tagcccacct cgtcgaccca gggccgcagg aagtcgaact tctccacgat
     1441 cgccttcttg acctcgaggt cgtcggtctc ctcggccgtg gcctcgatcc gcacctgccg
     1501 ctggcggtcc gcatcggcga agcacatctc caccgcgggc acgttgcaca attgcaggta
     1561 cacctccttg gtcttgcccg tgcagaagat gatgccccgg tcatcggcga acgagaccat
     1621 catgtagcgc acccgcgggc ggtcgccgtc gattgtgccc agggcgaaga tggggttttc
     1681 gttgatgaac tgaaggattt cgtctctggt catggcttgg cctttcgagt tcggtcctcc
     1741 cggttatggc ggcgtcccgt ccgccaccgg ctggccggca ggagccttgt g
//
LOCUS       DAOQTR010000481         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_87204_length_1784_cov_4.506651, whole genome shotgun sequence.
ACCESSION   DAOQTR010000481 DAOQTR010000000
VERSION     DAOQTR010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87204_length_1784_cov_4.506651"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKN85_10125"
     CDS             complement(<1..561)
                     /locus_tag="PKN85_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNT44812.1"
                     /translation="MDRLLVEKGFAETRTKAAALIMAGSVLINGAPVTKAGTLVDEDA
                     PITVKEPPRYVSRAGSKLEAALDAFGIDPSGKIAIDVGASTGGFTDVMLQRGAVKVYA
                     VDVGRGQLHWKLRSHGKVVSLEGVNVRNLDSSLIDDPCDLATFDVSFISLKLVIPPVL
                     KVLRPGSDLIALIKPQFEAGREHIGKG"
     gene            complement(570..>1784)
                     /locus_tag="PKN85_10130"
     CDS             complement(570..>1784)
                     /locus_tag="PKN85_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNT44813.1"
                     /translation="IPIGGDTLYKIAKLIEGNLKGAVTEGLLFEELGFRYVGPIDGHK
                     ISHLVETFQNISTMHEPIFLHVITQKGHGYSPAMRDPENFHGVGSFVRENGESRAGKN
                     TAVYSDVFGKTLTRIAKRDPRVVAISAAMTSGTGLKGFALKFPDRFYDVGIAEGHAVT
                     MAAGMALYGLRPVVAIYSTFLQRSYDEIIHDVALQNAPVVFAVDRAGLVGPDGPTHHG
                     VFDIAYFRSIPNVTILVPRDQFMLERMLIHAFKIQGPVAIRYPRDAVVPAPLKPGNLR
                     TCRAEVLKEGSRLAMFCCGPLCYSALEAAAAFEGVAVIDLVYAKPLDTQTVREMVKAC
                     GGRFMVIEDGCVHGGVGSAVLEALHDLGCPLKFRLLGVPDTFVEHGSLSELRKSLDLD
                     TAGIRRTVSELL"
BASE COUNT          343 a          547 c          534 g          360 t
ORIGIN      
        1 tcccttgccg atgtgctccc tccccgcttc gaactggggc ttgatgagag cgatcagatc
       61 ggagccgggt ctgagcacct tcagaaccgg aggaatgacg agcttcagcg agatgaacga
      121 gacatcgaac gttgccagat cacacggatc gtcgatgaga gaggaatcga gattccgtac
      181 attgacccct tcgaggctca cgaccttccc gtgggagcgg agcttccagt gaagctgtcc
      241 ccggcccacg tccaccgcat atacctttac cgcgccccgc tgaagcatga cgtcggtgaa
      301 tcccccggta gatgcgccga catcgatggc gatctttccc gagggatcga tgccgaaagc
      361 gtccagggcg gcttccagct tgcttcccgc ccggctcaca taccggggag gctctttgac
      421 cgtgatgggc gcgtcttcat ccaccagggt gcccgccttg gtcaccggcg ctccattgat
      481 gagcacactg ccggccatga tcagggcggc ggcctttgtt ctcgtttcgg cgaagccttt
      541 ttccaccagc agtctgtcca agcgcacctt cataagagtt cggagacggt tctccgtatt
      601 cccgccgtat cgaggtccag ggattttctc aactcgctca gggagccgtg ctcgacaaac
      661 gtgtcaggaa cgcccaggag cctgaatttc aagggacagc cgaggtcgtg gagggcctcg
      721 aggactgcgg accctacgcc gccgtgcaca cagccgtcct caatgaccat gaaacgtccc
      781 ccgcaggcct ttaccatctc acgtacggtc tgggtatcga gaggctttgc atagacgagg
      841 tcgatcacgg cgaccccttc gaaggcggca gcggcctcga gggcggaata gcacaagggg
      901 ccgcagcaga acatggcgag ccgtgatccc tccttcagta cttcggccct gcacgtcctc
      961 aggttgccgg gcttcaaggg agcgggcacg actgcatcgc gcggataccg gatggcgacc
     1021 ggcccctgga tcttgaaggc gtgaatgagc attctttcga gcatgaactg gtccctgggc
     1081 acgaggatag tgacattggg aatgctcctg aaataggcga tatcgaacac cccgtggtgc
     1141 gtggggccgt ccggtccgac gagtcccgcc ctgtcgacgg cgaaaaccac cggggcgttc
     1201 tgcagcgcaa cgtcgtggat gatctcgtcg tagcttctct gcaaaaacgt cgagtagatg
     1261 gctaccacgg gcctgagccc atagagggcc atgccggccg ccatggtcac cgcgtggcct
     1321 tcggctatgc caacgtcgta gaatctgtcc gggaatttca gggcaaaacc cttgagcccg
     1381 gtgcccgacg tcatggcggc gctgatggcg acgacccggg gatcgcgttt ggcgatcctg
     1441 gtgagtgtct ttccgaacac atccgagtag accgcggtgt tcttccccgc cctcgactcg
     1501 ccgttttccc gcacgaagct ccccaccccg tggaaatttt ccggatcgcg catagcgggt
     1561 gagtatccgt ggcctttctg ggtaatgaca tgaaggaaga tgggctcgtg catcgtggag
     1621 atattctgga aggtttccac caggtggctg atcttgtggc cgtctatggg ccctacatag
     1681 cggaagccca gctcctcgaa caaaagcccc tcggtcaccg cgcccttgag gttgccttcg
     1741 atgagcttgg cgatcttgta cagagtatcc cctccgatag gaat
//
LOCUS       DAOQTR010000482         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_87431_length_1782_cov_2.661262, whole genome shotgun sequence.
ACCESSION   DAOQTR010000482 DAOQTR010000000
VERSION     DAOQTR010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87431_length_1782_cov_2.661262"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKN85_10135"
     CDS             <1..627
                     /locus_tag="PKN85_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT44814.1"
                     /translation="YIGLKDFPGELLRAFQRRISGNENISLIQVSVEDGASTQKAQDD
                     IESLLRERRRISSLKPNDFQIRDMKELASTMLATTELLTTLLGAVAAVSLLVGGIGIM
                     NIMLVSVTERTREIGTRLAIGALEREVLLQFLVEAMVLSAFGGLFGIILALIGSAVLV
                     RVLDVPFVIDGWIIFIAFVFSAAVGLIFGYFPARKAASLDPIEALRHE"
     gene            637..>1782
                     /locus_tag="PKN85_10140"
     CDS             637..>1782
                     /locus_tag="PKN85_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNT44815.1"
                     /translation="MTRTIKETQGMNVTGKIMRYSAVFITVIILTGCVTVGPDYVKPD
                     IKAPEKWNSSQSAEASRADASKDSLASWWANLDDPTLVALIETALKNNKDLKQAASRI
                     REARAQRGVTEARLWPSVDGSGSYTRTRTENNSETGTSRELFAAGLDAGWEIDLFGGL
                     RRATEASQASYEASQEDYLSVHVSLAAEVALNYIDVRTLQERLAITEENLRLQTETWE
                     LTTYRLKAGLVTQFDVDRAKTNMEETRASIPTVVTRLAAAKNRLSTLMGEYPGYVDAK
                     LEEAKELPLAPVEIALGVPAETLRRRPDVRGAERELAAQTAKVGVATAELYPKLSLMG
                     SIGIDATSFAGLFSANNRGFGIGPRFSWNIFNAGAVRSNIEIQSARQE"
BASE COUNT          369 a          544 c          559 g          310 t
ORIGIN      
        1 tacatcgggc tgaaggactt ccctggcgaa ctgctgcgcg ccttccagcg ccgcatctcc
       61 ggaaacgaga acatcagtct catacaggta tcggtggaag acggcgcgtc gactcagaag
      121 gcccaggacg acatcgagag cctgctgagg gagcgccggc gcatctccag cttgaagccc
      181 aacgatttcc agataaggga catgaaggag cttgccagca ccatgctggc cacgacagag
      241 cttctcacga cccttctcgg ggcggtggcg gcggtcagcc ttctcgtggg gggcatcggg
      301 atcatgaaca tcatgctcgt gtccgtgacg gaacggaccc gtgagatcgg gacgcggctc
      361 gccatcggag cgctggaacg ggaagttctc ctgcagttcc tcgtcgaggc gatggtcctt
      421 tcggcgttcg ggggtctttt cggcatcatc ctggcactca tcggctcggc agttctcgtc
      481 cgggtcctcg atgtgccgtt tgttatcgac gggtggatca tcttcatcgc cttcgtgttc
      541 tcggcggcgg tgggcctcat attcggctat ttccccgccc ggaaggcggc gagccttgac
      601 ccgatagagg ccttgaggca cgaataggag agggccatga caagaacgat caaggagaca
      661 cagggcatga acgtgacagg gaagatcatg agatactcgg cggttttcat caccgtcatc
      721 attctgacag gctgcgtaac cgtcggaccc gattatgtca agcccgacat aaaggcgccg
      781 gagaaatgga actcctctca gtcggcagag gcctccaggg cggatgcctc gaaggactct
      841 ctcgcgtcat ggtgggcgaa ccttgacgat cccaccctgg tggcgctcat agagaccgcc
      901 ctcaagaaca acaaggactt gaagcaggcg gcgtcgagga tacgcgaggc cagggcccag
      961 cggggggtta ccgaggcgag gctgtggccg tcggttgacg gttccggttc ttacacgcgc
     1021 acccggacgg agaacaattc cgaaacgggc acatcgaggg aactctttgc cgccggcctc
     1081 gacgcgggat gggagataga cctcttcggt ggtttgagaa gagccacaga ggcatcgcag
     1141 gcctcttacg aggcgtcgca ggaagactac ctgtccgtgc acgtgagcct cgccgcggag
     1201 gttgccctta actacatcga cgtccggacc ctccaggaga ggttggccat cacggaagag
     1261 aatctgcgac tccagacgga gacctgggag ctaacgacct atcgcctcaa ggcgggcctc
     1321 gtgacccagt tcgatgtgga ccgggcgaaa acgaacatgg aagagacccg cgcgtccatc
     1381 ccgacggtgg tcacccgact tgccgccgcg aagaacaggc tttccaccct tatgggagaa
     1441 taccccggat atgtcgacgc gaagctcgaa gaggctaaag agctcccgct cgcccccgtt
     1501 gaaatagccc tcggggtccc cgcggagacg ctccgccgac gtcccgacgt gcgaggtgcc
     1561 gaaagggaac tggcagccca gacggccaaa gtcggtgtcg ccacggccga gctctatccc
     1621 aagctgagcc tcatgggctc cataggcatc gacgccacca gctttgcggg cctcttctcg
     1681 gccaacaacc gcggcttcgg catcggcccc cggttcagct ggaacatctt caacgccggc
     1741 gcggtgcgca gcaacatcga gatccagagc gcccgccagg aa
//
LOCUS       DAOQTR010000483         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_87549_length_1780_cov_4.131014, whole genome shotgun sequence.
ACCESSION   DAOQTR010000483 DAOQTR010000000
VERSION     DAOQTR010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87549_length_1780_cov_4.131014"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PKN85_10145"
     CDS             complement(<1..698)
                     /locus_tag="PKN85_10145"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNT44816.1"
                     /translation="MDISWGISEEIGFRPTMEDEHAFYEDPSRFFFSAEIYDGHGGRQ
                     PAQIAAEMLTPWFLHAWARQEERPLRERRDEADILREAYLAVDEHLVGRRVQAGTCAV
                     QLYIIGERFLAANVGDSRVIIGTDRGTAVLTDDHKPDATAERFRIEALGGSVVTLGVP
                     RVEGILAISRALGDACLKPYVSAEPRIAEGLLGSENDVAVLACDGVWDVLSPAEVIEA
                     ARQGLVPREGAEKIS"
     gene            812..1033
                     /gene="xseB"
                     /locus_tag="PKN85_10150"
     CDS             812..1033
                     /gene="xseB"
                     /locus_tag="PKN85_10150"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNT44817.1"
                     /translation="MKFEEGLKKLEDIVKTLDEGKIPLDEALGLFKDGLTLTKELSKR
                     LDEVEKKVEILIKKEDGTVEKAPFPGDDE"
     gene            1033..>1780
                     /locus_tag="PKN85_10155"
     CDS             1033..>1780
                     /locus_tag="PKN85_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT44818.1"
                     /translation="MDLASYLHDKKIIVENMLRDICTSFVSTPGILRNAMEYMLFSNG
                     KKIRPILAIAACEAKGKSADDFLPCFCTLEMIHTYSLIHDDLPCVDNDDTRRGRPTCH
                     KAFGEAVAIMAGDGLLTEAFRVLTDRRFTERVSPKISKQIVFEIAHAAGAEGMVGGQV
                     MDIIYENKEGTRNILHYIHSNKTAAMLRASVRIGAIAGGAGARELKSFTRYGDAIGLA
                     FQIMDDILDVEGDEETVGKKLKKDANKQTYV"
BASE COUNT          417 a          525 c          512 g          326 t
ORIGIN      
        1 gatatcttct ccgcaccttc ccgggggacc agcccctgcc gtgccgcttc gatcacctcg
       61 gcgggagaca ggacatccca taccccgtcg caggcgagga ccgccacgtc gttctccgag
      121 cccagaaggc cttcggctat gcgcggctcg gcgctcacgt agggtttgag acaggcatcc
      181 ccgagggccc ggcttatcgc cagaataccc tccacccttg ggacacccag agtgacaacg
      241 ctcccgccga gggcctcgat gcggaaccgc tcggccgtcg cgtctggttt gtggtcatcg
      301 gtgagaacgg ccgtcccccg gtccgtgccg atgattactc tggagtcgcc cacgttcgcc
      361 gccaggaagc gttcaccgat gatgtacagc tggacggcgc aggtccccgc ctgcacgcgg
      421 cggccgacga gatgctcgtc gacggcgaga taggcctcgc gaaggatgtc cgcctcgtct
      481 ctgcgctcgc gaagcggcct ttcctcctga cgcgcccagg catgcaggaa ccagggcgtc
      541 agcatctcgg cggctatctg cgcgggctgc cttccgccgt gcccgtcata tatctcagcg
      601 ctgaagaaga acctggaagg atcctcatag aaggcgtgct cgtcctccat cgtcggcctg
      661 aagcctatct cctcgctgat cccccaggaa atgtccatgg agatattgta ccccggattt
      721 cgtgtttttc acagggcgaa aggccgtccc gacggggcaa atcaatgctt gcaaaagcga
      781 atatttaagg tctataataa gtacttccat catgaaattt gaagaagggt tgaaaaaact
      841 ggaagatatc gttaaaaccc ttgacgaagg gaagataccc cttgacgagg cgctaggtct
      901 tttcaaggac ggcctcactc tcacgaaaga gctttcgaaa agacttgacg aagtcgagaa
      961 aaaggtggaa atactcatca agaaagaaga cgggacggtg gaaaaggcgc cctttcccgg
     1021 ggatgatgaa tagtggacct ggcgagctac ctccatgaca agaagataat agtcgagaac
     1081 atgctcagag acatttgcac cagcttcgtc tcgacccctg gcatcctgag gaacgccatg
     1141 gagtacatgc tcttcagcaa cgggaagaag atccggccca tccttgccat agccgcctgc
     1201 gaggcgaagg gaaagagcgc cgacgatttc cttccctgtt tctgcacatt ggagatgatc
     1261 cacacctatt ccctcatcca cgatgacctt ccctgtgtgg acaacgacga cacgcgccgc
     1321 ggcagaccga catgccacaa ggccttcggc gaggccgtgg ccatcatggc cggtgacggt
     1381 ctgcttacgg aggcgttcag ggtcctcacg gaccggcggt tcacggaaag ggtgagcccg
     1441 aagatatcga aacagatcgt gttcgagata gcccacgcgg ccggggccga agggatggtc
     1501 ggcggccagg tgatggacat aatatacgag aacaaggaag gcaccaggaa catacttcat
     1561 tacatacatt ccaacaaaac ggcggccatg cttcgtgcgt ccgttcgcat cggtgcgatc
     1621 gcaggcggag ccggggccag agaacttaag agcttcacac gctacggcga tgccatagga
     1681 ctcgccttcc agatcatgga cgacatcctc gacgtggaag gcgacgagga aacggttggc
     1741 aagaagttga aaaaagacgc caacaagcag acctacgtca
//
LOCUS       DAOQTR010000484         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_87735_length_1778_cov_4.100406, whole genome shotgun sequence.
ACCESSION   DAOQTR010000484 DAOQTR010000000
VERSION     DAOQTR010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87735_length_1778_cov_4.100406"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKN85_10160"
     CDS             complement(<1..224)
                     /locus_tag="PKN85_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT44819.1"
                     /translation="MQEELIKKATQYLANTYTRFPIVVTKGEGCWLWDVNGRRYLDFL
                     AGIAVCNLGHAHENVVEALTAQARKLFHVS"
     gene            complement(229..1068)
                     /gene="argB"
                     /locus_tag="PKN85_10165"
     CDS             complement(229..1068)
                     /gene="argB"
                     /locus_tag="PKN85_10165"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNT44820.1"
                     /translation="MSEKIQTLLEALPYISKFAGSIFVIKYGGAAMEEESLKKEFAKD
                     VALLKYVGINPVIVHGGGPMIGRLLKDLQIPTRFVNGLRVTDEKTLEVAEMVLSGLVN
                     KEIVKNINDMGGKAIGLSGKDGRLLMAKQVEDRDVGLVGEITSVDVAIIKDISRHGYI
                     PVIAPIADGVDGKAYNINADTAAGSIAAALSAEKFILLTDVQGVMGADGKLISVLKRS
                     EIEKLIDDNVVTGGMIPKVTCCTEALKGGVREAHVVDGRTPHAILLEVFTDSGIGTEI
                     TGD"
     gene            complement(1085..1654)
                     /locus_tag="PKN85_10170"
     CDS             complement(1085..1654)
                     /locus_tag="PKN85_10170"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44821.1"
                     /translation="MQTIAPTVPRYHGHGPDVSREGVQRDILPIVEGTTVTTKYGMVK
                     TDHILFIAAGAFHMSKPSDLIPELQGRFPIRVELDALTKDDFYRIITEPDNALIKQYR
                     ALLKTEDVELEFHEEAIREITDIAQRINEMTENIGARRLYTVMEKLLDDISFTAPDLK
                     GQKVEVTKSYVREKLGEFLDKEDLSRYIL"
BASE COUNT          314 a          560 c          484 g          420 t
ORIGIN      
        1 ttcgaaacgt ggaaaagttt ccttgcctgc gccgtcagag cctccaccac gttctcgtgg
       61 gcatgcccca ggttgcacac ggcgatgccg gccagaaaat cgagatatct ccttccgttg
      121 acatcccaga gccagcatcc ctcacccttg gtgacgacga tgggaaaacg cgtgtatgtg
      181 ttcgccagat actgcgtagc cttctttatc agttcttcct gcatctttct agtctcctgt
      241 tatctcggtg cctatccccg aatcggtgaa tacctcgagg agaatggcat ggggggtcct
      301 gccgtcgacg acgtgggcct ccctgacacc ccctttaagc gcctcggtgc agcatgtgac
      361 cttggggatc atcccgccgg tcacgacgtt gtcatcgata agcttctcta tctccgatcg
      421 cttcaggacg gaaatgagct ttccgtccgc ccccatgaca ccctgcacat cggtgagaag
      481 tatgaatttc tccgccgaga gggccgcggc gatgctgccc gccgccgtgt cggcattgat
      541 attgtatgcc ttgccgtcca ccccgtcggc gatgggagct atgacgggga tgtagccgtg
      601 cctgctgata tcctttatga tggcgacatc gacggaggtg atctctccca cgagacccac
      661 atccctgtcc tcgacctgct tggccatcag gagcctccca tccttgccgg atagtccgat
      721 ggccttgccg cccatatcat tgatattttt gacgatctcc ttgttcacga ggcccgacag
      781 gaccatttcg gccacctcca gggtcttctc gtcggtgacc ctcaagccgt tgacgaagcg
      841 cgtaggtatc tggaggtcct tgaggagcct cccgatcatg ggacccccgc cgtgaacgat
      901 gacggggttg atcccgacgt atttgaggag cgccacgtcc ttcgcaaact ccttcttcag
      961 ggattcctcc tccatggccg ccccgccata ttttatgacg aatatggacc cggcaaactt
     1021 gctgatatag ggaagtgctt ccagaagcgt ctgtatcttt tcgctcatca tggtaccccg
     1081 atcgttagag tatgtacctg ctcagatcct ctttgtccag gaactctccc agcttctccc
     1141 gcacatagga cttcgtcact tccaccttct ggcccttcag gtcgggcgcc gtaaaggaga
     1201 tgtcgtcaag gagcttctcc atcacggtgt agagcctccg ggcgcctatg ttctccgtca
     1261 tctcgtttat ccgctgggct atgtccgtga tctcccggat ggcctcctcg tgaaactcca
     1321 gttccacgtc ctccgtcttg aggagcgccc ggtactgctt gatgagggcg ttgtcgggct
     1381 ccgtgatgat ccggtaaaaa tcgtccttcg tcagggcgtc gagctccacc cggatgggga
     1441 aacggccctg cagctcgggg atgaggtccg agggcttcga catgtgaaag gcccccgcgg
     1501 cgatgaagag tatgtggtcc gtcttcacca tgccgtactt ggtggtgacc gtcgttccct
     1561 ccacgatggg gaggatgtcc ctctggacgc cctcgcgcga cacgtccggc ccgtggccgt
     1621 ggtacctcgg caccgtcggc gcgatggtct gcaaggcgat ggtctgcgac gattgcgatc
     1681 atcagttcga tttgaacaag ccccaggccg acctcgccac ccgcaagcgg aatctggcaa
     1741 tcgcgatcaa cggcatcggc ctggtcggca tcctcgcc
//
LOCUS       DAOQTR010000485         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_87997_length_1775_cov_3.132558, whole genome shotgun sequence.
ACCESSION   DAOQTR010000485 DAOQTR010000000
VERSION     DAOQTR010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87997_length_1775_cov_3.132558"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PKN85_10175"
     CDS             <1..1205
                     /locus_tag="PKN85_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT44822.1"
                     /translation="AMASRQNEIGDTIALEMGMPAAWSRLIQVGLPIATLDSFIPILE
                     SYEFEYSMGTTLVVKEAIGVCGFITPWNYPMHQIIGKVAPALAAGCTMVVKPSQLAPL
                     NSYILAEIMDEVGLPPGVFNLVVGAGSEVGHAIASHRDIDMVSLTGSTRSGVLVAQAA
                     AETIKRVTLELGGKSPNIILEDADITTAVLKGVFNCFLNSGQTCISLSRMIVPSHMHK
                     EIVELAKSTVESLKMGDAFEEGVYLGPMVDAQQQESVREYIRSGIREGATLVTGGPEQ
                     PESLKKGYFVKPTIFADVTPEMTIAQEEIFGPVLCIMPYRSEDEAVEIANNSIYGLSG
                     SVWSGDPDRARRVARRIRSGQVFINGSDFDINAPAGGYKQSGIGRERSKHGLDEYLEI
                     KALIGYNP"
     gene            1236..>1775
                     /locus_tag="PKN85_10180"
     CDS             1236..>1775
                     /locus_tag="PKN85_10180"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNT44823.1"
                     /translation="MAIRHAILGLLHYRPMHGYKIKAQIEQNFGHVWSINYGQIYPNL
                     KSLLDEGLISMTEENRAGMRGPSRKLYSITDKGRKAFSRWIMEPPDKNMTLRDPFLMK
                     FVFFGFGDRRRAVEMIDDQIRLYTEQLTSRKEKFKKLKNLEIHVRLISEMGIELNEAF
                     LAWLKRAREEIVRDAVNASN"
BASE COUNT          419 a          484 c          513 g          359 t
ORIGIN      
        1 aggccatggc ttcgcgccag aatgagatcg gcgacaccat cgccctggag atgggcatgc
       61 ctgcggcatg gtccaggctg atccaagtcg gtctccccat tgccacattg gacagtttca
      121 taccgatcct cgagagttac gagtttgagt actcgatggg gacaaccctg gtcgtcaagg
      181 aagccatcgg tgtctgcggg ttcataaccc catggaatta ccccatgcat cagatcatcg
      241 gcaaggttgc gccagcgctt gcggcaggat gtacaatggt cgtgaagccg agccagctcg
      301 ccccgctgaa ctcctacata ctcgccgaga tcatggatga agtcggtctc cctccggggg
      361 tcttcaatct cgtggtgggt gcaggatcag aggttggcca tgccattgcc tctcaccgtg
      421 atatcgacat ggtttcgctc accgggtcca ccaggtccgg cgtcctggtc gcccaggccg
      481 ctgccgagac catcaagcgg gttaccctgg agctcggggg aaaatccccg aacatcatcc
      541 tggaagacgc ggatatcacc acggcggtcc tgaagggagt cttcaactgc ttcctcaact
      601 cgggccagac atgcatctcc ctgtcgagaa tgattgttcc gtcgcatatg cacaaagaga
      661 tcgtcgagct cgccaaatca acagtcgagt ccctgaaaat gggcgatgcc tttgaggaag
      721 gcgtctatct cggccccatg gtcgacgctc agcagcagga gtcggtgagg gaatacatca
      781 gatcaggcat ccgcgaaggc gccaccctgg taacgggcgg tccggaacag cccgaaagcc
      841 tgaagaaggg atatttcgtc aagcccacga tctttgccga tgtaaccccg gaaatgacca
      901 tcgcccagga ggagatcttc ggtcctgttc tttgcattat gccataccgc agtgaggacg
      961 aggctgtcga gatcgccaac aacagtatct acggcctgtc aggctctgta tggtcgggag
     1021 acccggaccg ggcgaggagg gtcgcgaggc ggatccgttc gggccaagtc ttcatcaacg
     1081 ggtcggattt cgacatcaat gccccggcag ggggctacaa acagtccggt atcgggcgcg
     1141 agaggagcaa gcacggtctg gatgagtacc tggagatcaa ggcactcata gggtataatc
     1201 cgtaatcact ttctcatgca ggagggtgct cattcatggc gataagacac gcgatactcg
     1261 ggctgctcca ttaccggcct atgcacggct ataagatcaa ggcgcagatc gaacagaatt
     1321 tcggccatgt atggtccatc aattacggcc agatctatcc gaacctgaaa tctctgctgg
     1381 atgaaggact catcagcatg accgaggaaa accgggcggg aatgaggggg ccgagccgaa
     1441 agctgtattc catcacggac aagggcagga aggccttctc ccgctggatc atggagcccc
     1501 ccgataaaaa catgactttg agggatccct tcctcatgaa atttgtcttt ttcggcttcg
     1561 gcgaccggcg gcgggctgtc gaaatgatcg atgatcagat caggctttat acagaacagc
     1621 tcacatccag gaaggagaag ttcaagaaac tgaaaaacct tgagattcat gttcgcctga
     1681 tctccgagat ggggatcgaa ctcaacgagg cctttctcgc atggctgaaa agggcgcgcg
     1741 aagaaatcgt gcgggatgcc gtgaatgcat cgaat
//
LOCUS       DAOQTR010000486         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_88552_length_1768_cov_3.385873, whole genome shotgun sequence.
ACCESSION   DAOQTR010000486 DAOQTR010000000
VERSION     DAOQTR010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88552_length_1768_cov_3.385873"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PKN85_10185"
     CDS             complement(<1..1266)
                     /locus_tag="PKN85_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNT44824.1"
                     /translation="MMERVCYHRETVESLCESLKSRSTGLGSDEAARRLDEYGPNELK
                     AKKKKTPVMMFLDQFKDFMILILLGAAVVAGVIGELTDTLIIIAIVIANAVIGFVQEF
                     RAEKAIEALKQMAAPMAAVLRDSNVRSIASRDIVPGDVVLLEAGVIVPADMRLIESAQ
                     MRVDEAALTGESVPVEKSVGELCDADVSIGDRVNMVYKGTIVTYGRGMGLVVGTGMET
                     ELGRIASMLQDEDEVRTPLQKRLTVFGKNLAIAILIICAVVFVIGLMRGERPLLMLLT
                     AISLAVAAIPEALPAVITISLALGAKNMVKKNALIRKLPAVETLGSVTYICSDKTGTL
                     TYNRMTVEELYARGEVVGSREDRARGLADLMRGIALNNDSREGTDGAFIGDPTEVALY
                     TAARDNGYERERIETDFPRVGEIPFDSDRK"
BASE COUNT          332 a          532 c          504 g          400 t
ORIGIN      
        1 cttgcgatcg gaatcaaagg ggatctcgcc cacgcggggg aagtccgtct cgatcctttc
       61 cctctcgtac ccgttgtcgc gggccgcggt gtagagggcg acctccgtgg gatcgccaat
      121 gaaggctccg tcggtgcctt ccctggagtc gttgttcagg gcgatgcccc gcatgagatc
      181 cgcaagtccg cgggccctgt cctccctgct cccgacgacc tctccccggg cgtagagctc
      241 ttcgacggtc atgcggttgt acgtgagcgt ccccgtcttg tcggagcaga tgtacgtgac
      301 ggacccgagc gtttccaccg cggggagctt cctgataagg gcgttctttt tcaccatatt
      361 cttcgcgccc agcgcgaggg agatggtgat gaccgccggc agggcctctg gtatggccgc
      421 gacggcgaga gaaatggccg tcaggagcat gagcagcggc cgctctcccc gcatgaggcc
      481 gataacgaag acgacggcgc atatgatgag gatggctatg gcaaggttct ttccgaagac
      541 cgtcagccgt ttctgaaggg gggttctgac ctcgtcctca tcctggagca tggaagctat
      601 ccttcccagt tccgtctcca tgccggtgcc gacaacgaga cccatgcctc tgccgtaggt
      661 gacgatagtt cccttgtaga ccatgttcac cctgtcgccg atggagacat ccgcgtcaca
      721 aagctctccg acggatttct ccacgggaac ggattccccc gtgagggcgg cctcgtcgac
      781 gcgcatctgg gcgctttcga tgaggcgcat gtccgccgga acgatgacgc ccgcttcgag
      841 aagaacgacg tcgcccggaa cgatgtcccg cgaggctatc gaccggacgt tgctgtctct
      901 caggacggcc gccatgggag cggccatctg tttcaaggcc tcgatggcct tttccgccct
      961 gaattcctgg acgaaaccga tgacggcgtt ggcgatgacg atggctatga tgatgagcgt
     1021 gtccgtcagt tccccgatga cgcccgcgac gacggcggca ccgaggagga tgaggatcat
     1081 aaagtccttg aactggtcga ggaacatcat caccggcgtt ttcttcttct ttgccttcag
     1141 ctcgttgggg ccatattcat cgagccttct cgcggcctcg tcgctcccga ggcccgtgct
     1201 ccggctcttc aagctttcgc aaagggattc caccgtttcc ctgtggtaac aaacacgctc
     1261 catcattctc tcctctcctg tgatacgatc ggccctggtg cgggtcggtc gttctggcaa
     1321 aggtctcgct tgttagaatc tctaaccggc ataccggtcg gttttaaggc cgacgtattg
     1381 acgatggtgc cgatgaagct actccccttt gatggcaatc cctgtcaacc catggagtat
     1441 tgtatacctc acttcctcac gagctgcaaa gtcgggcatg taccgccctc cagatgtctt
     1501 tcgcgcccga agtggacaca aaaaaagaga cgctttaaca tgaacataac cttcatgcca
     1561 aaggtctcgc tcgatcggtc ccttgcggtg tgaccgaaga cagggccagg attctcactc
     1621 cggattgttg accccgtctc ttatagctac tccccttcag ggctggggta ttatactgca
     1681 gtctgcgcag gaattcaacg gtcctgtcac tttcgaattg cctcataaaa aatgttaccc
     1741 aaggggtggt gagagaaaga tcgttgac
//
LOCUS       DAOQTR010000487         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_88697_length_1766_cov_3.446523, whole genome shotgun sequence.
ACCESSION   DAOQTR010000487 DAOQTR010000000
VERSION     DAOQTR010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88697_length_1766_cov_3.446523"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKN85_10190"
     CDS             <1..768
                     /locus_tag="PKN85_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT44825.1"
                     /translation="ERWRPGAFVGMTGMETGCSFEFTAGAMGREDACPPEMELALPGQ
                     LCYNRPVWVPAVIDNAEQDEREASDLAYISRVLAGDRDAFAFLLKKYKMYVLKIVSRH
                     VPYEDAEEVAHDAFIRVYESLAKFSASGGFRHWIAAIATRTCYDYWRRAYRSKEITMS
                     SLGDAHQEWLEKAAADPSGGSIDDIGREVEAREILDWALARLSPEDRMVLELVYLEGL
                     SVREVADLLGWSTANVKIRSFRSRRKLQKLLKDAGGK"
     gene            781..1104
                     /locus_tag="PKN85_10195"
     CDS             781..1104
                     /locus_tag="PKN85_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44826.1"
                     /translation="MGSPKDERERLEAALAAAYRSRGGPEPGEEWESQVMRGVRNLPN
                     RVAGASLTDLFGSLFWRVCPVACAIVIFLAVAVFRYDTAAEQDLAQIITNDTVETVLL
                     DPYNS"
     gene            1118..>1766
                     /locus_tag="PKN85_10200"
     CDS             1118..>1766
                     /locus_tag="PKN85_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44827.1"
                     /translation="MNKIKIVITLALVLLVGVFAGALGTRIYLRHQTEGSHATRSRTS
                     GERVTQIMGRLTKDLKLDAVQRAEIEKVVLKTDGKATAVKVSYQPELKKIYDQGFEAI
                     KERLNEEQKAKLRARHERFSQRYNALYFRSLRAAQRGIPDADALKDRLGLDTAQHARV
                     ATILADQKNRQGFIIARYEKAENPDLFAVNSDLEEVRRSVRKQLAEALTPEQLARY"
BASE COUNT          396 a          479 c          586 g          305 t
ORIGIN      
        1 gaaagatgga ggccaggggc cttcgttggg atgacaggga tggagacggg atgctcattt
       61 gagtttacgg cgggagcaat ggggcgggaa gatgcatgcc cgcccgagat ggaacttgcc
      121 ctgccgggtc agctttgtta taacagacct gtatgggtac ctgccgtgat cgataatgcc
      181 gaacaggacg aacgcgaagc gtcggacctt gcgtacatca gccgtgttct cgccggtgat
      241 cgcgacgcct ttgcttttct tctcaaaaaa tacaagatgt acgtcctcaa gatagtcagt
      301 cgccacgtcc cctacgagga tgccgaagag gtggcgcacg acgccttcat tcgcgtctac
      361 gagtccctgg caaagttcag cgcgtccggg gggttcaggc actggatagc cgccatagcg
      421 acgcggacct gctacgacta ctggcgacgc gcgtacaggt cgaaagagat caccatgagc
      481 tccctgggtg acgcgcatca ggaatggctg gagaaggccg ctgccgatcc gtcgggaggc
      541 tccatcgacg acatcggcag agaggtggag gcgcgggaga tacttgactg ggcgctggcc
      601 aggctctcgc cggaggacag gatggtgctg gagcttgtct acctggaagg cctgtccgtg
      661 cgcgaggtcg ccgacctcct cggatggagc acggcgaacg tgaagatacg ttccttccgg
      721 tcacggagga agctgcagaa gctcctcaag gacgccgggg ggaaatgaag gaggcacgaa
      781 atgggatccc cgaaagatga aagagaaagg ctggaggcag ccctcgcggc cgcgtaccgg
      841 agccggggcg ggccggagcc gggcgaggaa tgggagtcac aggtcatgag gggcgtgagg
      901 aacctgccca accgggttgc aggggcttcc ttgacggacc tgttcggaag tctcttctgg
      961 cgggtctgcc ccgtggcatg cgccatcgtc atcttccttg cggtggccgt gttccgttat
     1021 gacacggcgg cagagcagga tcttgcgcag atcatcacca acgacaccgt cgagacggtc
     1081 cttctggacc cctacaacag ttgacaggag gcctgacatg aacaagataa agatcgtcat
     1141 caccctggcg ctggtccttc tggtgggggt ctttgccggc gcgttgggaa cccggatata
     1201 tctcaggcac caaacggaag gatctcatgc tacgcggtcc cggacctccg gggagagggt
     1261 aacgcagatc atggggaggc tcacgaagga cctgaagctg gatgccgtgc aacgggccga
     1321 gatagaaaag gtggtcctga agaccgacgg gaaggcgacc gcggtcaagg tgtcttacca
     1381 gccggagctc aagaagatat acgaccaggg tttcgaagcc ataaaagaga ggctcaacga
     1441 ggaacagaag gcaaaactgc gggcgcgaca cgagaggttc tcccagcggt acaacgcgct
     1501 gtatttcagg tccctgcgtg ctgcccagag gggaattccc gatgccgacg ctctgaagga
     1561 ccggctcggt ctcgataccg ctcaacacgc gcgggtggcc accatcctcg cggaccagaa
     1621 aaacaggcag ggtttcataa tcgccaggta tgagaaggcg gagaaccccg acctctttgc
     1681 cgtcaactcg gaccttgaag aggtgcgacg ttccgtaagg aaacaacttg ccgaggcgct
     1741 gacaccggaa caactggccc gttatg
//
LOCUS       DAOQTR010000488         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_89177_length_1760_cov_4.063343, whole genome shotgun sequence.
ACCESSION   DAOQTR010000488 DAOQTR010000000
VERSION     DAOQTR010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89177_length_1760_cov_4.063343"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKN85_10205"
     CDS             <1..354
                     /locus_tag="PKN85_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT44828.1"
                     /translation="PRQLRIVSYSLSASKWETIKKVVIPAAIPGILTGIMLSIGRAVG
                     ETAAIIFTMGSSLRLPLSLMDSGRTMAVHFYILAREGISMEKAYGTAVVLVLSILLIN
                     ILAYYIMNKAIARYS"
     gene            362..1117
                     /locus_tag="PKN85_10210"
     CDS             362..1117
                     /locus_tag="PKN85_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNT44829.1"
                     /translation="MDTKIATDRLRLSFGTTEVLKSVTIKVHRNTITGFMGPSGSGKS
                     TLISVINRMIDFENNVKIEGDATIDGTSILDGSINPIELRRRVGTVFAVPIPLPRSIF
                     ENIAYGPRLKGTSDRTTLHHIVEDSLKKAFLWDEVKDRLGTSALKLSGGQQQRLCLAR
                     TLALKPEIILLDEPCSGLDPISTAKIEDALSELKSEYTIILVSNNTKQIARISDFSAF
                     FYLGELIEYDTTEKVFTAPAEAKTEDYIQGKFG"
     gene            1117..>1760
                     /locus_tag="PKN85_10215"
     CDS             1117..>1760
                     /locus_tag="PKN85_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNT44830.1"
                     /translation="MDRFALRTNKLNLYYGDFHALKNVDFQVHSKAITALIGPSGCGK
                     STLLRCFNRMNDLLEEVRIEGEIYVHNEKLQDIDIINLRKKVGMVFQRPNPFPFTVYE
                     NMVYGLKVHGIGHRRGWREIVDRCLHSVGLLDELKDKLHTPALDLSEEIKQRLCVARL
                     LTVEPEIILLDEPCSALDPIATMRIEELMIELKERYTILIVTHNMQQAARVSDYT"
BASE COUNT          433 a          541 c          452 g          334 t
ORIGIN      
        1 ccccggcagc tcagaatagt gagctactcc ctgagcgcga gcaagtggga gacgatcaag
       61 aaggtggtca ttccggcggc gatccccggg atacttaccg gcatcatgct cagcatcggg
      121 cgggccgtgg gcgaaacggc ggcaatcatc ttcacaatgg gcagttccct gaggcttccc
      181 ctgtccctca tggattcggg caggaccatg gcagtccact tctatattct ggcccgggaa
      241 ggcatttcca tggagaaggc gtacggcacg gccgttgtcc tcgtgctcag catccttctc
      301 atcaacatac tcgcgtacta catcatgaac aaggccattg cgcggtattc atagggaaga
      361 aatggacaca aagatcgcga ctgacagatt acgattatcc ttcggcacga cagaggtgct
      421 caaaagcgtc accatcaagg ttcacaggaa caccatcacc ggtttcatgg ggccctcggg
      481 tagcggcaaa tcaaccctca tctccgtcat caaccgcatg atagacttcg agaacaacgt
      541 gaagatcgag ggcgatgcca ccatagacgg aacgagcatc cttgacggga gcatcaaccc
      601 cattgagctg cgaagacgtg taggaaccgt tttcgccgtt cccatccccc tgccccgttc
      661 catattcgag aacatcgcct atgggcccag acttaaaggg acgagcgacc gcaccaccct
      721 gcaccacatc gtagaggaca gcctcaagaa ggcattcctc tgggatgagg tgaaggacag
      781 gctcggcacg tcggcgctca agctctccgg cggacagcag caaaggctct gcctcgcgcg
      841 gaccctggcg cttaaaccgg agatcatcct tctcgatgag ccttgctccg gcctcgaccc
      901 catctcaacg gcaaagatcg aggacgccct gagtgagctg aagtcggagt acaccatcat
      961 cctcgtgtcc aacaacacga aacagatcgc ccggatcagc gacttttcgg ctttcttcta
     1021 cctcggggag ctcatagagt atgatacgac ggaaaaggtc tttaccgccc ccgccgaggc
     1081 caagaccgaa gactacatac agggaaagtt tggataatgg atagattcgc cctgagaacg
     1141 aacaagctca acctctatta cggggatttt cacgccctca agaacgtcga tttccaggtc
     1201 cactccaagg ccataacggc cctcatcggc ccgtcgggat gcggaaagtc aacgcttctt
     1261 cgctgtttca accgtatgaa cgatcttctg gaggaggtcc gcatcgaggg cgagatatac
     1321 gtccacaacg agaagctgca ggatatcgat atcatcaacc tgaggaagaa agtgggcatg
     1381 gtctttcagc gcccaaaccc cttccccttc actgtttacg agaacatggt ctacggcctc
     1441 aaggtccacg gaataggaca caggcgcgga tggagagaga tcgtcgatcg ctgtcttcac
     1501 tccgtcggcc ttctcgacga gctcaaggac aaactccaca cccccgccct cgatctctcg
     1561 gaagagataa agcagcgtct ttgcgtcgcc cgcctcctga cggtggaacc ggagataatc
     1621 ctcctcgacg agccctgctc cgccctagac cccatcgcca ccatgaggat agaagagctc
     1681 atgatcgaac tgaaggagcg ctacaccatc ctcatcgtca cccacaacat gcagcaggcc
     1741 gcccgagtct ccgactacac
//
LOCUS       DAOQTR010000489         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_89360_length_1758_cov_3.137992, whole genome shotgun sequence.
ACCESSION   DAOQTR010000489 DAOQTR010000000
VERSION     DAOQTR010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89360_length_1758_cov_3.137992"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PKN85_10220"
     CDS             <1..819
                     /locus_tag="PKN85_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT44831.1"
                     /translation="SYACDAFPLKDYLSPGMIMPYSTSAGCYWGKCSFCPETAEGTPY
                     AQLAPIEAAAGLRELAEGYRPALAHIVDNAISPSVLKALVNEPPGVPWYGFARVTKEL
                     TDEGFCRDLRASGCVMLKLGVESGDQDVLDGMRKGIGISDVSKALKALTRAGIGTYVY
                     LLFGTPGEDEEAARRTMDFIVEHHEFIGFVNLAVFNLPHSSPDAAALDAKPFYGGDLS
                     FYTDFVHPKGWDRRKVRRFIDREFTRHPAIREIVNRQPPYFTSNHAPFFVDAFC"
     gene            853..1686
                     /locus_tag="PKN85_10225"
     CDS             853..1686
                     /locus_tag="PKN85_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT44832.1"
                     /translation="MKDILKRTVRHHLALVGLIILVPMFFCAVFAPVISPHDPIEPDI
                     KNILSAPTFSHPFGTDTLGRDVFSRVLYGTRISLLVGFVSVGIAILIGLILGAISGYF
                     GGMVDEVIMRFVDLMMCFPTFFLILAVIAILEPSIWNIMIVIGLTSWMGTARLVRAEI
                     LSIKSKEYVMAAKAQGFNSLRIIFRHVLPNAVTPVYVVATLGIGGAILTESALSFLGI
                     GVQPPTPSWGNILTQAKDNIEVAWWLSLYPGLAIFLTVMGYNLLGEGLRDVFDPRRKG
                     A"
BASE COUNT          327 a          571 c          490 g          370 t
ORIGIN      
        1 tcctatgcct gcgatgcatt ccctttgaag gattacctgt cgccgggtat gatcatgcct
       61 tacagcacat cggcgggatg ctactggggc aaatgctcct tctgtcccga gacggccgaa
      121 ggcaccccat acgcgcagct cgcgccgata gaagccgcgg ccgggctgag agagcttgct
      181 gaggggtacc ggcccgccct ggcacacatc gtcgacaacg cgataagccc ttccgtcctc
      241 aaggcgctcg tcaatgaacc gcccggcgtt ccctggtacg gctttgcccg ggtgacgaag
      301 gagctgacgg acgagggttt ctgcagggac ctcagggctt ccggctgcgt catgctcaag
      361 ctcggcgtcg agtcgggaga ccaggacgtc ctcgacggca tgcgcaaagg gatcggaatc
      421 tccgacgtgt cgaaggcctt gaaagcactt acgcgggcgg gtatcggcac ctacgtctac
      481 ctccttttcg ggacacccgg agaggatgag gaggcggctc gcaggaccat ggatttcatc
      541 gtggagcacc atgagttcat cgggtttgtc aacctcgccg tcttcaacct tccccactca
      601 agccccgacg ccgccgccct cgacgcgaaa cctttctacg gcggcgacct ctcgttctac
      661 acggacttcg tccatccgaa gggatgggac cgcaggaagg tgaggcgctt catcgacagg
      721 gaattcacac gccaccccgc catacgggag atcgtgaacc ggcagccccc ctacttcaca
      781 tccaatcatg cgcctttctt cgtagacgct ttttgttgat tttaaatcct gcggctatta
      841 atatgaggtg acatgaagga tatccttaag aggacggtac gccatcacct cgcgctcgtg
      901 gggctcatca tcctcgtgcc catgttcttc tgcgccgttt tcgcgccggt catttcccct
      961 cacgacccca tcgagcctga catcaagaac atcctctcgg cgcctacctt ctcccatccc
     1021 ttcggcacgg acacgctggg acgggacgtc ttctcccggg tcctctacgg gacgcggata
     1081 tccctcctcg tgggattcgt ttccgtgggc atcgccatcc tcatagggct cattctcggc
     1141 gccatatcgg gttatttcgg ggggatggtg gacgaggtca tcatgcgctt cgtcgacctc
     1201 atgatgtgtt tcccgacgtt cttcctcatc cttgccgtca tcgccatcct ggaaccaagc
     1261 atctggaaca tcatgatcgt catcgggctg acgagctgga tgggcacggc gcgcctcgtc
     1321 cgggcggaga tactgagcat aaagagcaag gagtatgtca tggccgcgaa ggcgcagggc
     1381 ttcaattccc tgaggatcat cttccgccac gtcctgccca acgcggtgac ccccgtctac
     1441 gtcgtcgcga cgctgggtat cggcggggcc atcctgaccg aatcggcgct gtccttcctc
     1501 ggcatcggcg tccagccccc cacaccgagc tggggcaaca tcctcaccca ggcgaaggac
     1561 aacatagagg tggcctggtg gctctccctc taccccggcc ttgccatctt cctcaccgta
     1621 atgggctaca acctcctcgg cgaaggcttg agggacgtct tcgatccaag gcgcaaggga
     1681 gcctgaaaga aagggcttga atggcttgaa tcgcttgaca gcctgttgat aaaccccacc
     1741 agccggccct cttttccc
//
LOCUS       DAOQTR010000490         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_90038_length_1750_cov_2.987611, whole genome shotgun sequence.
ACCESSION   DAOQTR010000490 DAOQTR010000000
VERSION     DAOQTR010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90038_length_1750_cov_2.987611"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKN85_10230"
     CDS             complement(<1..611)
                     /locus_tag="PKN85_10230"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNT44833.1"
                     /translation="MSTQKLKEIIKQVLPDVEAVICWQEGFDKLNVTPIFITSPEQAE
                     KVVWNPLCAQNLASYLPSQKKKVAVAVKGCDSRTIVQYMQEGLIDRNNVVIIGIPCKG
                     IISKKKVQKAIGNEPVEDVSFTEGSVKVKTPSGEKTFAIADVCPSKCTSCQYPTPIVY
                     DQLTADPIQSDKPAESVYADVAELEGKTLEERKAYFLSQFDKC"
     gene            complement(624..1040)
                     /locus_tag="PKN85_10235"
     CDS             complement(624..1040)
                     /locus_tag="PKN85_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT44834.1"
                     /translation="MSEVAPKELRIVGFLCNWCSYGGADTAGVGRFRQPTDLRIIRVP
                     CSGRVDPMFVVRALLTGADGVLVSGCHPRDCHYTDGNFYARRRLEMLKRLLPFLGIDD
                     KRFHYTWVSASEGAKWQQVVTDFTKQVHALGPMHQK"
     gene            complement(1028..>1750)
                     /locus_tag="PKN85_10240"
     CDS             complement(1028..>1750)
                     /locus_tag="PKN85_10240"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT44835.1"
                     /translation="ILTKDHIPDSQSYVFYMDVRSPGKGYDEFTRRAQEEYGAKYIRG
                     RVSRIYPKGKKMVVRGADTLLGTQVEIEADLVVLGTAVVAAPGAPQMAEKLHISYDTF
                     GFYVESHPKLRPVETNTSGVYLAGACQGPKDIPASVGQGSAAAAKVLSLFSKDMLESD
                     PAIAQVNQNTCVGCLKCLKTCPFQAIVEDTLRNGKKVAKVIETVCAGCGVCTSTCPCG
                     AIQLQHFTDNQLLAEVNAICQK"
BASE COUNT          339 a          517 c          505 g          389 t
ORIGIN      
        1 atgcacttgt cgaactggct caggaaatat gccttgcgct cctccagtgt cttgccttcc
       61 aactcggcaa cgtcagcgta aacgctttcc gccggtttgt ccgactgtat cggatccgcc
      121 gtaagctggt catagacgat cggcgtcggg tactggcacg aggtacactt gctgggacag
      181 acatcggcta tcgcgaaggt cttctcgccc gacggcgtct tcaccttgac agaaccctcc
      241 gtgaagctca catcctcaac gggctcgttc cctatcgcct tctggacctt tttcttgctg
      301 atgatgccct tgcaggggat gccgataatg acgacgttgt tcctgtcgat gaggccttcc
      361 tgcatatact ggacgatggt cctcgagtcg cagcccttca ccgcgaccgc caccttcttc
      421 ttctgcgagg ggaggtagct ggcgaggttc tgggcgcaca gggggttcca cacaaccttt
      481 tcggcctgct caggggacgt tatgaagatc ggcgtcacat tgagcttgtc aaaaccctcc
      541 tgccagcata tgacggcctc cacatcgggg aggacctgct tgatgatctc tttaagcttc
      601 tgagtactca atctgccttc tccttacttt tgatgcatgg ggccgagcgc gtggacctgt
      661 ttggtaaagt ccgttacgac ctgctgccat ttcgcgcctt cagaggccga aacccatgta
      721 tagtggaatc tcttgtcgtc gatgcccagg aacggcagga gccttttgag catttccaac
      781 ctgcgccgcg cgtagaagtt gccgtcggtg taatggcaat ccctggggtg acacccggag
      841 acgagaacac catcggcacc cgtaagaaga gcgcgcacga cgaacatggg gtcgacgcgt
      901 cccgagcagg ggaccctgat gatcctcagg tccgtgggct gcctgaacct gcccacgccc
      961 gcggtgtcag caccgccgta ggaacaccag ttacagagaa atccaacgat ccgcagttct
     1021 ttgggtgcta cttctgacat attgcattaa cctccgctaa gagctggttg tccgtgaaat
     1081 gctgcagctg gatagcaccg cagggacagg tggatgtaca gacaccgcat ccggcacaga
     1141 ccgtttcgat gaccttggcg accttcttgc cgttcctcaa ggtgtcttca acgatggcct
     1201 gaaacggaca ggtcttgagg cacttgagac agcccacgca tgtattctgg ttgacctggg
     1261 caatggccgg gtcggattcg agcatgtcct tagagaaaag cgacaggacc ttcgcggcgg
     1321 cggcggaacc ctgtccgacc gatgccggga tgtccttggg gccctggcag gcgccggcca
     1381 ggtagacacc ggaggtgttc gtctccaccg gcctgagctt cgggtgagac tcaacgtaga
     1441 acccgaaggt atcgtaggag atgtggagct tctcggccat ctgcggggcg cccggggcgg
     1501 cgacaaccgc tgtcccgagg acgacgaggt cggcctcgat ctcaacctgg gtcccgagaa
     1561 gcgtatcggc acccctcacg accatcttct tacccttcgg gtagatccgc gataccctgc
     1621 cgcggatata cttcgcgcca tactcttcct gggcgcggcg tgtaaattca tcgtaacctt
     1681 tgcccggtga cctgacgtcc atgtagaaga cgtaggactg ggaatccggg atgtggtcct
     1741 tggtcaggat
//
LOCUS       DAOQTR010000491         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_90569_length_1744_cov_2.791593, whole genome shotgun sequence.
ACCESSION   DAOQTR010000491 DAOQTR010000000
VERSION     DAOQTR010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90569_length_1744_cov_2.791593"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKN85_10245"
     CDS             complement(<1..803)
                     /locus_tag="PKN85_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44836.1"
                     /translation="MQRPQAWMEREGLKRGGWCFVIAWTVWILLVYAVGGYFGVRLLQ
                     SYKMQTREHQQAWMRTFTGPGQDASIPAAGTAPAEEPVDVHAAVSLNRISDIELREAN
                     WTAEFDIWFRWERGSVDPGRNLRIVNGTILEKEKIAAGLRNGMRYEQYRLKARISKVF
                     DAARFPFSNEGLAIGLTDGIEGTDKLRFVRDGRGIDISHLALPQGIRIRKEVAVVGLH
                     RYATGEAGTGDHAGADRTHSVLVVGLLVVPPQREHLSETLPGPLRLCCD"
     gene            complement(824..>1744)
                     /locus_tag="PKN85_10250"
     CDS             complement(824..>1744)
                     /locus_tag="PKN85_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44837.1"
                     /translation="STRHVTQYFEMMGHRSLYHDGWRAVCPWPGPSFTEAGKFFGTPI
                     PKGTLTELDANNWELYHVEKDWTENHNIASENRAKLIEMVGTWYVEAGKYKVMPVDGR
                     GTQRFAEERPQIAKDRTSYTFYPGTQSVPYNAGPRLLNRTHSITADVEVPEKGAEGVL
                     ISFGGTDGGYALYLMDGKLQYVQNYVAREYLHVASTEPVPPGHHQLRFEFEATGGPDI
                     GQGRGSPGHVELYMDGKLVGAADFALTTPLILGLTGGIKVGADPGAPVAPFYDAPFEF
                     TGTICSVTIDVSGEVIKDDEAELRAIMARQ"
BASE COUNT          312 a          550 c          497 g          385 t
ORIGIN      
        1 tgatcgcaac agaggcgaag agggcctgga agagtttcag atagatgctc tcgctggggg
       61 ggtacaacca gaaggcccac aacaaggacc gaatgggtcc tgtccgcgcc cgcatggtct
      121 ccggtacctg cttcccccgt ggcgtacctg tgcaacccga ctaccgccac ttccttcctg
      181 atcctgatgc cttgaggcag ggccaggtga cttatatcaa taccgcggcc gtcgcggaca
      241 aatcgcagtt tgtctgtccc ttcgatcccg tccgtcaacc cgatggccag gccttcgttg
      301 ctgaaaggga aacgcgcggc atcgaagacc ttgctgatac gggccttaag gcggtattgc
      361 tcatagcgca ttccattcct gaggccggcc gcgatctttt ccttttccag gatcgtgccg
      421 tttactatcc gaaggttccg gccgggatcc acggaccctc tttcccagcg gaaccatatg
      481 tcaaattcag ctgtccagtt cgcctccctg agctcgatat cgctgatgcg gttaagggag
      541 acggccgcat ggacatcgac cggttcttcg gcaggtgctg ttccggcagc gggaattgaa
      601 gcatcctgcc ccggcccggt gaaggtccgc atccatgcct gctggtgctc ccttgtttgc
      661 attttatagc tttgaagcag cctgacgccg aaatagccgc cgaccgcgta gacgagaagt
      721 atccaaaccg tccacgcgat cacaaagcac cagccgcctc gcttcaaccc ctccctttcc
      781 atccacgcct gcggtctctg cacggtcgtg tctccttcag ccgtcactgc cgcgccatga
      841 tcgccctcag ttccgcttca tcgtccttta tgacctctcc gctgacgtcg atggtcacgc
      901 tgcaaatcgt gcccgtgaac tcaaacggcg cgtcatagaa aggtgcaaca ggtgctcccg
      961 ggtcggcgcc gaccttgatg ccccccgtca gcccaaggat aagcggagtg gtcagcgcga
     1021 agtcggctgc gccgacaagt ttgccgtcca tgtaaagctc gacgtgtccg ggcgagcccc
     1081 tgccctgccc tatatcaggg ccccctgtgg cctcgaactc aaaacgcagt tggtggtgcc
     1141 ccgggggtac cggctcggta gaggctacgt ggagatattc acgggctacg taattctgca
     1201 cgtactgcag cttgccgtcc atgagataca gagcgtaacc accgtccgtc ccgccgaagg
     1261 agatcaacac tccctccgcc cctttttcgg ggacctccac gtcggccgtg atgctgtggg
     1321 tccggttgag gaggcggggt cccgcattgt acgggacgga ctgggtgccg ggatagaacg
     1381 tgtagctcgt gcggtctttg gcgatctgag ggcgctcctc tgcgaagcgt tgtgtgccgc
     1441 ggccgtcgac tggcatcacc ttgtacttgc ctgcttccac gtaccaggta cccaccattt
     1501 cgatgagttt ggccctgttc tccgaggcaa tgttatggtt ttccgtccag tccttctcga
     1561 cgtgatacag ttcccagttg ttggcatcca gttcggtcag agtccctttc ggtatcgggg
     1621 tcccgaagaa cttgcctgct tccgtgaacg aaggcccggg ccaggggcag acggcccgcc
     1681 atccgtcgtg atagagggag cggtgcccca tcatctcgaa gtattgcgtg acgtgcctag
     1741 tgga
//
LOCUS       DAOQTR010000492         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_90804_length_1741_cov_2.798932, whole genome shotgun sequence.
ACCESSION   DAOQTR010000492 DAOQTR010000000
VERSION     DAOQTR010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90804_length_1741_cov_2.798932"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..966)
                     /locus_tag="PKN85_10255"
     CDS             complement(199..966)
                     /locus_tag="PKN85_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNT44838.1"
                     /translation="MDILTELIGSLREDAPVGEVRRGLHWTAVVSKRCGLASMLGSAG
                     GCNHEMTGGMERSFTEMTALELARDCSLSNDTALLSLGLAAVNSLIDVDMNRCGDAEG
                     LQLAGDMGKGKNIAVIGHFPYLADVARVARNLWVIEKRPHPGDFSEEQGKDLIPQSDI
                     VVISSTTIINRTLPGILGLCREGAVKMLLGPSTPLTESLFDLGMDILSGSVVTDKDSV
                     LRSVSEGISFMQLKKRGGVRFVTMVKDYDDILRRLKG"
     gene            1216..>1741
                     /locus_tag="PKN85_10260"
     CDS             1216..>1741
                     /locus_tag="PKN85_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNT44839.1"
                     /translation="MGLACRVAVLSAFFILLSKPLLASGDGGSGLVTSVGIAIIAATV
                     MSFLGHMMRQPLLLAYIAAGVLIGPNIGLGLVTDASDIETISHIGLVLLLFLIGLEID
                     IKKLKESGRSLVVTGLFQFILCVLLGLGFFYLLGFTVGGGRYDLIYLAACCALSSTAI
                     VVKLLYGKFEIDTLA"
BASE COUNT          340 a          503 c          481 g          417 t
ORIGIN      
        1 gtcctgaaga tttggatacg gcaggcccgc cgcgaaggtg cctgagacct tttcatgata
       61 gcagtgggtt aacccccctg ctcaagcagg atgtcaacag gggtgagcag ggggggtatc
      121 ggaggtccag cgatgaacgg ccgctggatt atgaaacggc tatgccgggg gaagacgccg
      181 ggtggtacgg gcactttctt atcccttcag ccttcgaaga atgtcatcgt agtccttgac
      241 catggtgacg aagcggacgc ctcctctctt cttgagttgc atgaaactga tcccttcgct
      301 caccgatctc agcacactgt ccttatccgt cacgacgctt cccgagagga tgtccatgcc
      361 caggtcgaag agggattccg tcaggggcgt gcttggcccg aggagcatct tgacggcccc
      421 ttccctgcac agtccaagga tgcccgggag ggttcggttg atgatggtgg tgctggagat
      481 gacgactatg tcggattgag ggataagatc tttgccttgc tcttccgaga aatcacccgg
      541 atgcggtctc ttttcgatca cccagaggtt ccgggcgacc ctggcgacat cggcaaggta
      601 cgggaaatgg ccgataacgg cgatgttctt tcccttgccc atatctcccg ccagttgcag
      661 cccttccgcg tcgccgcacc tgttcatatc gacatcgatg agggaattta cggccgcaag
      721 gccgagggac aacagggccg tgtcattgct caggctgcag tcgcgggcga gttcgagggc
      781 cgtcatctcc gtgaaagacc tttccatgcc tcctgtcatt tcgtgattgc atcctcccgc
      841 cgaacccagc atggaagcca ggccgcatcg tttgctcacc acggcggtcc agtgcaatcc
      901 ccttcgtact tccccaaccg gtgcatcttc ccggagggac ccgatcaatt ccgtcagaat
      961 gtccatatca tttcctctcc aacttgctgc cgaattgagc ccatctcttg tcgttatata
     1021 gcacactata taacgaaaaa gcaactgcaa agactgcatc gatccccgat ggctgaagcg
     1081 cgggcattgt ccttgaaaaa tgtgcgcgtt cctgatagcc tagcctagat gggcatcata
     1141 gactcctgct gcaggcgtac aggagagatg tgaaggggct gaacgaatac attgcgaaca
     1201 actaggagat atcccgtggg gctggcatgc cgcgtggctg tcctgtcagc ttttttcatt
     1261 ctactttcga agccactcct ggcttccggt gatggcggca gcggtcttgt caccagtgtc
     1321 ggcatagcca tcatcgccgc gaccgtcatg tccttcctgg gccacatgat gcgtcagcct
     1381 cttctgctcg cgtatatagc cgccggcgtt ctgatcggtc ccaacatcgg tctcggactg
     1441 gtgaccgacg caagcgatat cgagaccata tcccacatcg gtctcgtcct ccttcttttc
     1501 ctcatagggc ttgagatcga catcaagaag ctgaaggaat cgggcaggtc cctcgttgtc
     1561 acgggcctct ttcagttcat cctgtgcgtt ctgcttggac tcggcttctt ctatcttctt
     1621 ggttttaccg tcggtggcgg gagatatgac ctcatctacc ttgccgcgtg ctgtgccctg
     1681 agcagtacgg ccatcgtcgt gaagctgctg tacggaaagt tcgagataga caccctggcg
     1741 g
//
LOCUS       DAOQTR010000493         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_91015_length_1738_cov_3.418301, whole genome shotgun sequence.
ACCESSION   DAOQTR010000493 DAOQTR010000000
VERSION     DAOQTR010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91015_length_1738_cov_3.418301"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PKN85_10265"
     CDS             <1..1274
                     /locus_tag="PKN85_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT44840.1"
                     /translation="TSALSIAKQGHEVWLVEKENELGGMVRKLYYTLEGLDVQAFLKD
                     LVAKVYNHPMIHVYTGATIPEATGYVGNFVTKIESKRGTVEIKHGATVVAIGADLYTP
                     TEYLYGEDDRVMSHLELEERIAAGDEKVVGAKSAVMIQCVGCRNEERNYCSRICCSES
                     IKNALLLKERNPDMDVCVLFRDIRTYGFKEDYYREAAGKGVRFIRYEPDKKPLVEPGK
                     AEDGRDVLKVTAPDYVLGKGIELDADLLVLAAAIIPSAASQEVSKLFKVALGPDGFCQ
                     EAHVKLRPVDFAAEGVYLCGTAHYPKLMSEAISQAYGAAGRALTLLANDTVVASGSVC
                     AVNEKQCMGCGACAPACTYNAIELRETKQGRKAEVIPVLCKGCGLCNSKCPTGAIQLK
                     HYTDEEIFSQILAGLHKGDIMEQQDEAVGNA"
     gene            1333..>1738
                     /locus_tag="PKN85_10270"
     CDS             1333..>1738
                     /locus_tag="PKN85_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT44841.1"
                     /translation="MSSEGQFKPKMLGIICNWCCYGGADLCGVSRFQYPTYIRLIRVM
                     CSGRVDLKHILLAFANGVDGMFVGGCHINDCHYNPEGNYDAMSMVNLCKKLLEHIGIN
                     PKRLRLEWVSAGEGIRFANIMNEFAREIEELGP"
BASE COUNT          414 a          497 c          546 g          281 t
ORIGIN      
        1 tgacgagcgc cctgtccatc gcgaagcagg ggcacgaggt ctggctggtg gagaaggaga
       61 atgaactggg agggatggtg cgcaagctct actacaccct cgaggggctc gacgtccagg
      121 ccttcctcaa agacctggtc gcaaaggtct acaaccaccc catgatacac gtttacaccg
      181 gcgccaccat cccggaggcc accggctacg tcggtaactt cgtgacgaag atagagtcaa
      241 agcgcgggac cgtcgagata aagcacggtg ccacggtcgt cgcgataggc gccgacctgt
      301 acacgccgac ggaatacctc tacggggaag acgacagggt gatgagccac ctggaactgg
      361 aggaaaggat cgccgcaggt gacgaaaagg tcgtcggcgc gaagagcgcg gtcatgatcc
      421 agtgcgtggg ctgcaggaac gaggagagga actactgcag caggatatgc tgcagcgagt
      481 ccatcaagaa cgcccttctc ctgaaggaaa gaaaccccga catggatgtc tgcgtcctct
      541 tccgggacat acggacatac gggttcaagg aggactacta ccgggaggca gcggggaaag
      601 gggtcaggtt catccgctac gagccggaca aaaaacccct ggtggaaccc ggcaaggcgg
      661 aagacggccg ggacgtcctc aaggtgacgg ccccggacta tgtcctcggg aaagggatcg
      721 aactcgatgc tgacctcctt gttctggcgg cagcaatcat cccctcggcg gcaagccagg
      781 aggtctccaa gctcttcaag gtggccctcg ggcccgacgg cttctgccag gaggcccatg
      841 tgaagctaag acccgtcgac ttcgccgccg aaggggtata tctctgcggg acggcccact
      901 acccgaagct aatgtccgag gccataagcc aggcatacgg ggccgcgggc cgcgccctga
      961 cccttctggc gaacgatacc gtggtggcct ccggctccgt ctgcgccgtc aacgagaaac
     1021 agtgcatggg atgcggggcc tgtgccccgg cctgcacgta caacgccatc gagcttcgtg
     1081 agacaaaaca gggaagaaag gccgaggtca tccccgttct ctgcaaggga tgcggcctgt
     1141 gcaactcgaa gtgccccacg ggggcgatcc agctcaaaca ttataccgac gaggagatct
     1201 tcagccagat actcgcgggg ctccacaagg gcgacatcat ggaacagcaa gatgaggcgg
     1261 tcggaaatgc ttaagagagt gcatcagcgt accgctcgaa tacaggatat gacatcaggg
     1321 aggtaagaga ggatgagttc agaaggccaa ttcaaaccga agatgctggg tatcatatgc
     1381 aactggtgct gttacggcgg ggcggacctt tgcggggtct cccgttttca ataccccacg
     1441 tacatacgat tgatacgggt gatgtgctcc ggaagggtcg acctcaagca catactcctc
     1501 gccttcgcga acggggtgga cggaatgttc gtcggcggct gccacatcaa cgactgccat
     1561 tacaaccccg agggcaatta cgacgcgatg agcatggtga acctctgcaa gaagctcctc
     1621 gagcacatcg gcatcaaccc gaagagactg cgcctcgaat gggtctccgc cggcgagggg
     1681 atccgcttcg ccaacatcat gaacgagttc gccagggaga tagaggaact ggggcccc
//
LOCUS       DAOQTR010000494         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_91320_length_1735_cov_2.417262, whole genome shotgun sequence.
ACCESSION   DAOQTR010000494 DAOQTR010000000
VERSION     DAOQTR010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91320_length_1735_cov_2.417262"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKN85_10275"
     CDS             <1..374
                     /locus_tag="PKN85_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44842.1"
                     /translation="SDFDKASFLVALNHHERWDGKGYPGYIDFKTGEPLRGFEDLSGR
                     AIGKKEEEIPIFGRIVAIADVFDALSSGRSYKEPWEEERVLETMDRERGKHFDPEMLD
                     VFFSILDVIRNIMQRYPDNNE"
     gene            complement(512..1636)
                     /gene="alr"
                     /locus_tag="PKN85_10280"
     CDS             complement(512..1636)
                     /gene="alr"
                     /locus_tag="PKN85_10280"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT44843.1"
                     /translation="MSSSPRAVAYIDLGALEENCRSIRLCLEPGTGLLGVVKADAYGH
                     GALEVSKRLESVGIDYLGVATVDEGVDLRKGGIATPLLIMSGVFSWEEIEPVVRYDLT
                     PVIYDDALLGRFVALSREFARPVRVHVKFDTGMGRLGFKPAEAQRVAAALADAPGIRT
                     EGIMTHFPSPEVRDEYGLSQVRTFTKVVDTFRTAGLDPAYVHMSSSGAIVTYPEARFS
                     LVRAGISLYGSHPSRSLEEALPLRQVMKVVARIAFCREFPAGVPLSYGRTYQTPRDTR
                     IAYIPVGYSDGYPRSLSNKGRVLIKNRECNIVGRVCMDWTLADVTGVDGVESGDEAIL
                     LGEGDSRGTRVSANEMAELAGTIPYEILCKISRRIKRVYI"
BASE COUNT          345 a          551 c          504 g          335 t
ORIGIN      
        1 gctccgattt cgacaaggcc tccttccttg tcgccctcaa ccaccacgaa aggtgggacg
       61 gcaagggcta ccccggctac atcgacttca agacaggcga gcccctgcga gggttcgagg
      121 acttaagcgg cagggccatc ggcaaaaaag aggaggagat ccccattttc ggccgcatcg
      181 tcgccatcgc cgacgtcttc gacgccctga gctcgggaag gtcctacaag gaaccctggg
      241 aggaggagcg cgtcctcgaa acgatggacc gtgaacgggg caaacacttc gaccccgaaa
      301 tgctcgacgt cttcttctcc atcctcgacg tcatccgcaa catcatgcaa cggtatccgg
      361 ataacaacga gtagaggcgg cttgaatagc ttgaatcgct tgaaggtgaa agacaagaag
      421 attgcttctg tggcttgtcc ggggccacgc ataacccaag gtattctttt ggcctttaac
      481 tctcaagcca ctcaagcgat tcaagccgtc tttagatgta cacccgtttg atccttctcg
      541 atatcttgca gagtatttcg taggggatgg ttccggccag ttcggccatt tcgttggcgg
      601 aaacgcgtgt gccgcggctg tcgccttcgc cgaggaggat cgcctcgtcg ccgctctcga
      661 cgccgtcgac gcccgtcacg tcggcgaggg tccagtccat gcacaccctg ccgacgatgt
      721 tgcattccct gttcttgatg aggacacggc ccttgttcga cagggaccgg gggtatccat
      781 cggagtatcc cacgggtatg tatgctatcc gcgtgtcccg gggtgtctgg taggtccttc
      841 cgtagctcaa cggaacccct gcgggaaact ccctgcagaa cgcgatgcgg gccaccacct
      901 tcatgacctg cctcaaaggg agggcctcct cgagggagcg cgacgggtgg gaaccataga
      961 ggcttatccc cgcccggacg agggagaacc gggcctcggg ataggtcacg atggcgccgc
     1021 tcgagctcat gtgcacgtac gccgggtcga gacctgccgt cctgaaggta tccacaacct
     1081 tcgtgaaggt cctcacctgg gaaagtccgt actcgtcacg tacctccggc gagggaaagt
     1141 gcgtcatgat gccttccgtt ctaatccccg gcgcgtccgc aagagcggcg gcgaccctct
     1201 gcgcctcggc aggtttgaag ccgagccgtc ccatgcccgt atcgaacttc acgtgcaccc
     1261 tgacgggccg cgcgaactcc ctggagaggg cgacaaacct tccgaggagg gcgtcatcgt
     1321 agatgacggg cgtgagatcg taccggacga caggctcgat ctcctcccag gagaagacgc
     1381 cgctcatgat cagaaggggc gtcgcaatcc cgcccttcct gaggtccacg ccttcgtcga
     1441 ccgttgccac ccccaggtag tcgatgccga cggattccag cctcttcgat acctcaaggg
     1501 ccccgtggcc gtaggcatcc gccttcacga caccgagaag cccggtgccg ggctcaaggc
     1561 acagcctgat ggaccggcaa ttttcctcca gggcgccaag gtcgatgtag gcgaccgccc
     1621 tgggagagct cgacattgaa agcctccttt attgctgcag cgcggaaccg attctgccca
     1681 acaccttctc gcatgccgca agagtccgtt cgatctctgt ttgacccagc gcaag
//
LOCUS       DAOQTR010000495         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_92252_length_1724_cov_4.016777, whole genome shotgun sequence.
ACCESSION   DAOQTR010000495 DAOQTR010000000
VERSION     DAOQTR010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92252_length_1724_cov_4.016777"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /gene="gdhA"
                     /locus_tag="PKN85_10285"
     CDS             <1..1060
                     /gene="gdhA"
                     /locus_tag="PKN85_10285"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNT44844.1"
                     /translation="GILKFLGFEQIFKNSLTTLAMGGAKGGSDFDPKGKSDNEVMRFC
                     HAYMLELFRHIGPDIDVPAGDIGVGGREIGYMYGMYKKIRNDHTGVLTGKGLPFGGSL
                     VRPEATGYGCVYFAAEMLATKGLDFKGKTVAISGSGNVAQFAVEKVNQLGGRVVTLCD
                     SSSTIVDGEGISGDKCGYVMDLKNVQRGRISEYADKFGCAVCYIGESVWDVIKSQGLK
                     VDIALPCATQNEIDGSHAEALVKNGCVCVAEGSNMPSTPEAVRVFQDNGLLFGPGKAA
                     NAGGVATSGLEMSQNSMRLSWTREEVDARLLGIMKSIHKSCLDAAEVYGKKGDYVFGA
                     NVAGFLKVANAMLAYGYV"
     gene            1118..>1724
                     /locus_tag="PKN85_10290"
     CDS             1118..>1724
                     /locus_tag="PKN85_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HNT44845.1"
                     /translation="MNRIMFSILSVLDREGAPLGSSDIASRLVRQGTDLSERTIRYYL
                     KRLDEAGYTVTDGKKGRRITAGGRAELRQGYVSDRVGFIINRINNLAFLSDFGPDNVR
                     GKVILNVTFVPEERLEAAIEVLGVALRSPYSMSERAVVVRAGEHIGTVEAPAGTAAIG
                     TMCSITLNGMFLKRGIPIATKLGGLVEVVDGRSLRFTSVISY"
BASE COUNT          361 a          484 c          563 g          316 t
ORIGIN      
        1 ggggatcctc aagttcctcg ggttcgagca gatattcaag aattccctca cgacgctcgc
       61 catgggcggc gccaagggcg gtagtgattt tgaccccaag ggcaaatcgg acaacgaggt
      121 gatgcgtttc tgtcacgcct acatgctgga gctcttcagg cacatcggtc ccgacatcga
      181 cgtgcccgcc ggagacatcg gcgtgggagg gcgcgagata ggttacatgt acggcatgta
      241 caagaagatc aggaacgacc atacgggggt tctcaccggt aagggcctgc ccttcggcgg
      301 cagcctcgtc aggcccgagg cgacgggata cggctgcgtc tacttcgccg cggagatgct
      361 ggccacgaaa ggcctcgact tcaagggcaa gaccgttgcc atcagcggtt ccgggaacgt
      421 cgcccagttc gcggtggaaa aggtgaacca acttggcgga agggtcgtca ccctctgtga
      481 ctccagctcc accatcgtcg atggggaggg gatatcgggc gacaagtgcg gttacgtcat
      541 ggatctcaag aatgtccaga ggggcaggat aagcgagtac gccgacaagt tcggctgcgc
      601 cgtctgctac ataggcgaaa gcgtctggga cgtcatcaag agccagggtc tcaaggtcga
      661 catagctctg ccgtgcgcga cccagaacga gatagacggc agccacgcgg aagcgctcgt
      721 gaagaacggc tgcgtctgcg tcgccgaagg ctccaatatg ccttcgactc ccgaggcggt
      781 ccgcgtcttc caggacaatg gcctgctctt cggccccggc aaggcggcga acgccggagg
      841 agtcgcgaca tcgggcctcg agatgtccca gaacagcatg aggctttcct ggacacggga
      901 agaggtcgac gccaggctgc ttggcatcat gaagagcatc cacaagtcat gcctcgatgc
      961 cgccgaagta tacggcaaga aaggggacta tgtcttcggt gccaacgtcg ccggtttcct
     1021 caaggtcgcg aacgccatgc tcgcttacgg ttacgtgtga taaggaccgg tgaatggagc
     1081 ttttttgtgt aaaactctgc gcggagggaa tgggtagatg aacaggataa tgttctccat
     1141 tctctccgtg cttgacaggg aaggggcccc cttgggctcc tccgatattg cctcccgtct
     1201 tgtccgccag gggacggacc tgtcggagcg gaccatcagg tattacctga aacgtctcga
     1261 cgaggccggg tacaccgtga cggacggcaa gaaaggcagg aggataacgg caggcggccg
     1321 cgcggagctc aggcaggggt acgtgtccga ccgggtgggc tttatcatca acaggataaa
     1381 caacctcgcc ttcctgtccg atttcgggcc cgacaacgtg cgcgggaagg tgatactcaa
     1441 tgtcaccttt gttcccgagg agaggctcga ggcggccata gaggtcctgg gcgtcgcgct
     1501 caggtcgcct tactccatga gtgagcgggc ggtcgtcgtc agggccgggg aacatatcgg
     1561 cacagtggaa gcgccggcag gaacggccgc catagggacc atgtgcagca tcaccctcaa
     1621 cgggatgttc ctgaagcggg gcatccccat agcgaccaag ctcggcggtc tcgtcgaggt
     1681 ggtcgacggg cgctctttgc ggttcacatc ggtgatcagc tatg
//
LOCUS       DAOQTR010000496         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_92255_length_1724_cov_3.852007, whole genome shotgun sequence.
ACCESSION   DAOQTR010000496 DAOQTR010000000
VERSION     DAOQTR010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92255_length_1724_cov_3.852007"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKN85_10295"
     CDS             <1..380
                     /locus_tag="PKN85_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44846.1"
                     /translation="GVSFYNDSKATNVDATKRALEGMEGGIVLIAGGKDKGGSYRVIR
                     EEAGKIKALIAIGEARDRIASELGDAIPTYLEKDMAAAVSLAFAKAGQGDSVILSPMC
                     SSFDMFRDYKDRGNTFRRIVEAL"
     gene            377..1165
                     /locus_tag="PKN85_10300"
     CDS             377..1165
                     /locus_tag="PKN85_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44847.1"
                     /translation="MKKVLIAIFSLLLVYGLLREYTVARVLVVIAGAAAGYAIFRLPQ
                     RSIEAMKYPLIVISFLVTILFFFYPRIAIPEAARMAIVFVSFYAFILYLTGMEEKGQD
                     FFKEVTALSILFFSSGFNLYITGKLVFMISFAATLVLFLFIIGRYRIIPFIAAYASIA
                     AFMAYRQGAVLTGSGLTGLAEINRYVLLGSAFALLVTSFAFVMKKSSFATMLPFFGFL
                     YIAMDILLVVGVRFSTGLLYQPVLFIAILAPLAGMMLKTEGGKS"
     gene            1162..>1724
                     /locus_tag="PKN85_10305"
     CDS             1162..>1724
                     /locus_tag="PKN85_10305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT44848.1"
                     /translation="MRVVISAGGTGGHIFPGIAVAETLMGRGGDNSVLFIGTPYGLEG
                     RIVPDAGFRLRLIEAHQFLGRSPIHKAVTILRMMKGVIMCLKILKEERPDAILGMGGF
                     TSVPVIVAGRMLAIPCFIHEQNVEPGLANKLLSKITRKVFVSFDETKKHLPAGHGVHT
                     GNPLRSKLKKLEGLKEPGSFGIFVFGGS"
BASE COUNT          324 a          539 c          493 g          368 t
ORIGIN      
        1 gcggtgtttc cttctacaac gactccaagg caacaaacgt ggacgcaacg aagagggccc
       61 tggaggggat ggaaggcggc atcgtactca tcgcgggagg aaaggacaag ggcggaagtt
      121 accgggtcat ccgcgaagag gccggtaaga taaaggccct catcgccata ggcgaggcac
      181 gggataggat agcgtccgag ctcggagacg ccattcccac gtatctcgag aaggacatgg
      241 cagcggctgt ctccctcgcg ttcgcgaagg ccgggcaggg agactccgtc atcctgtccc
      301 cgatgtgcag cagcttcgac atgttcaggg attacaagga caggggaaac actttcagga
      361 ggatagtgga ggcgctgtga aaaaggtcct catagccata ttctcgctcc ttctcgtgta
      421 cggtttactc cgggagtaca cggtggcaag ggtcctcgtc gtcatcgccg gggcggcggc
      481 gggctacgcc atcttccgcc ttcctcagcg ctccatcgaa gccatgaagt accccctcat
      541 cgtcatctcc ttcctggtca ccatcctgtt cttcttctac cccaggattg ccatccccga
      601 ggccgcgcgg atggcgatcg tcttcgtctc cttctatgct ttcattctgt atcttacggg
      661 catggaagag aagggacagg acttcttcaa ggaggtcacg gccctgtcga tcctcttctt
      721 ctcgtcgggc ttcaaccttt acatcacggg caaactcgtc ttcatgatat ccttcgcggc
      781 gacgctcgtc cttttcctct tcatcatcgg acggtatcgg atcatcccct tcatcgccgc
      841 ctacgcctca attgcggcct tcatggcata ccgtcagggt gctgttctca ccggcagcgg
      901 gctcacgggc ctcgccgaga tcaacaggta cgtgctcctc gggtcggcct tcgcccttct
      961 cgtcacgagt ttcgccttcg tcatgaagaa gagctccttc gccaccatgc tgcccttttt
     1021 cggtttcctt tacatcgcca tggacatcct tctcgtcgtg ggggtcaggt tctccacggg
     1081 gctcctctac cagccggtcc tcttcatcgc catccttgcg ccccttgcgg ggatgatgct
     1141 gaagaccgaa ggggggaagt catgagggtc gtcatatctg caggcgggac aggcggacac
     1201 atcttccccg gaattgccgt cgccgagacg ctcatggggc gcggcgggga caacagcgtc
     1261 ctcttcatcg gtacgcccta cggtcttgaa gggaggatcg tccccgatgc cgggttcaga
     1321 ctccgcctca tcgaggcgca ccagttcctg gggcgctctc ccatccacaa ggcggtcacc
     1381 atcctgagga tgatgaaggg cgtcatcatg tgcctcaaga tcctgaagga ggagcggccc
     1441 gacgccatcc tcggcatggg cgggttcacg tcggtgcccg tcatagtcgc gggaaggatg
     1501 ctcgccattc cctgcttcat ccacgaacag aacgttgagc cggggctcgc gaacaagctc
     1561 ctctccaaga tcacccgcaa ggtcttcgtg agcttcgatg agacgaaaaa gcacctcccg
     1621 gcggggcacg gcgttcacac cggcaatccc ctgcggagca agctcaaaaa gctcgagggc
     1681 ctcaaagagc cgggctcctt cgggatattc gttttcggcg gctc
//
LOCUS       DAOQTR010000497         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_92541_length_1721_cov_3.045018, whole genome shotgun sequence.
ACCESSION   DAOQTR010000497 DAOQTR010000000
VERSION     DAOQTR010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92541_length_1721_cov_3.045018"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKN85_10310"
     CDS             <1..630
                     /locus_tag="PKN85_10310"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNT44849.1"
                     /translation="NAGDAVATCVGPLGNATTIDTFGDVLLVGGCYGIGSLYPVAKEL
                     KAKGNKVSVVLEARSSYLIYWLNKYVPLAEKVYTITRDGSLGQKGHISRLPDVIHGMP
                     RKPDRIIVNGCTYLMAHTSEVTKDLDIPVVVNLNPIMIDGTGMCGVCRVTVAGQMKFA
                     CVDGPEFDGHDIDWKEFLARRKAYVTEEAMFFRRSDAPAHNEGKCAWHE"
     gene            623..>1721
                     /gene="gltA"
                     /locus_tag="PKN85_10315"
     CDS             623..>1721
                     /gene="gltA"
                     /locus_tag="PKN85_10315"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNT44850.1"
                     /translation="MNEEKTQEKASKIDLNRRDMPRQSPEMRRNNFDEVALGYTEELA
                     RAEASRCLQCKKPRCVTGCPVEIDIPGFIGCITRGDFKGGIKKLKEKNCLPAVSGRVC
                     PQESQCESKCVLGNKKAEIAIGRLERFLADWEAGQGKVDLPPRAKSTGKKVAVVGSGP
                     AGLTVAGDLILLGHEVTIFEALHKAGGVLVYGIPEFRLPKAIVQREVDYLKALGVEVF
                     TDFVVGKTRSIDELLEEFDAVFVGTGAGLPWFMNIPGENLNGVYSANEYLTRMNLMKG
                     YLYPKSSTPVKKHVKVAVVGGGNVAMDSARTALRMGAAESHIVYRRSHAELPARAEEA
                     ENAEEEGVIFDLLTLPLAYIGDENGWVKEIEC"
BASE COUNT          378 a          470 c          571 g          302 t
ORIGIN      
        1 aacgccgggg acgcggtggc gacctgcgtg ggccccctgg gcaacgcgac aaccatcgat
       61 acattcggag atgtcctgct ggtgggaggg tgttacggga tagggagcct ctatcccgtg
      121 gcaaaggagt tgaaggcgaa ggggaacaaa gtgagcgtcg tgctcgaggc caggagttcc
      181 tacctgatct actggctcaa caagtatgtc ccgctggccg agaaggtcta caccatcacg
      241 cgggacggga gtctgggtca gaaaggccac atcagccgcc tgcccgacgt catacacggc
      301 atgccccgga agcccgacag gataatcgtc aatggatgca cctatcttat ggcgcacacg
      361 tcggaggtca cgaaagacct tgacataccg gtcgtcgtta accttaatcc tatcatgatc
      421 gatggcaccg ggatgtgcgg tgtgtgccgg gtcaccgtcg cgggccagat gaagttcgcc
      481 tgcgttgacg gtcccgagtt cgacggacac gacattgact ggaaggaatt tctcgcgcgc
      541 aggaaggcgt acgtgacgga agaggccatg ttcttccgca ggagcgacgc acccgcgcat
      601 aacgaaggga agtgtgcctg gcatgaatga agaaaagacc caagaaaagg caagcaagat
      661 agacctcaac cgcagagaca tgccgaggca gtctccggaa atgaggcgga acaatttcga
      721 tgaggtggcg ctgggctaca ccgaggaact ggcgcgggcg gaggcctcgc gctgtctcca
      781 gtgcaagaag ccgcggtgcg tcacgggttg ccccgtcgag atagacatcc ccggttttat
      841 cgggtgtatc acgaggggcg atttcaaggg gggcataaag aaactgaaag agaagaactg
      901 cctgcccgcg gtttcggggc gggtctgccc ccaggaatcc cagtgcgagt cgaagtgcgt
      961 cctcggcaac aagaaggcgg agatcgccat cggccgcctt gaacgctttc ttgccgactg
     1021 ggaggcgggg cagggcaaag tggacctgcc gcccagggca aagtctacag gcaagaaggt
     1081 ggctgtcgta ggttccggtc cggcggggct caccgtcgca ggcgacctca tcctcctcgg
     1141 ccatgaagtg acaatcttcg aggcgcttca taaggccggc ggagtcctcg tctacggtat
     1201 cccggagttc cgtctgccca aggcgatcgt tcaacgggaa gtggattacc tgaaggccct
     1261 cggggtggag gtgttcaccg acttcgtcgt aggaaagacg aggtccatag acgaactgct
     1321 cgaggagttc gatgcggtct tcgtggggac cggggcgggg cttccctggt tcatgaacat
     1381 tcccggcgag aacctgaacg gcgtttattc ggcgaacgag tacctgacgc gcatgaacct
     1441 catgaagggc tacctctatc cgaagtcgtc tacaccggtc aagaagcatg tgaaggtcgc
     1501 ggtggtcggc ggcggcaacg tggcgatgga ctccgcccgg acggccctgc gcatgggcgc
     1561 cgcggagtcg cacatagtct accggcgttc ccatgccgag ctcccggcgc gtgccgaaga
     1621 ggcggagaat gccgaagaag aaggcgtgat cttcgatctg cttaccctgc cgctcgccta
     1681 cataggcgac gagaatggct gggtcaagga aatcgaatgt a
//
LOCUS       DAOQTR010000498         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_92566_length_1721_cov_2.414166, whole genome shotgun sequence.
ACCESSION   DAOQTR010000498 DAOQTR010000000
VERSION     DAOQTR010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92566_length_1721_cov_2.414166"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1721)
                     /locus_tag="PKN85_10320"
     CDS             complement(<1..>1721)
                     /locus_tag="PKN85_10320"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT44851.1"
                     /translation="ILGLGKGENLTILAPIVRGRKGEYRKEIDELRQHGFTKVRIDGA
                     VMDLADDIELDKNKKHEIEVVVDRITIREGVERRLFEAVELALSRAGGLVKVETGSQA
                     RVFSEKFACPDCGISYPEIAPRMFSFNNPYGACPACTGLGVKEYFDPALIVPNQDLSL
                     RDGAVVPWAEKNPVHFLPFLEALVKHYRIDIRKPFRSLPKDVREAILYGSKGEEIEFF
                     HDHGGRRSFSRRPFEGVIGELTRDYEDADFYEREKLERFINLIPCPECNGARLRKEML
                     WVRVGGLNIFEVSRMTIEECATFFDAIELPEKEQEIARTILKEVTSRLKFLLDVGLDY
                     ISLSRNSATLSSGESQRIRLATQIGSGLTGVLYVLDEPSIGLHQRDNDRLLQTLRRLR
                     DLDNTVIVVEHDYDAIVSSDYVVDLGPGAGEKGGHLVFQGTPAELKMHPESVTGLYVS
                     GRRSIERPAGRRQPRGSLTVKGARANNLKNIDVAIPLGVFTAVTGVSGSGKSTLVVDT
                     LYPALKQKLYRSKERVGESDGILGFEAVDRVIDIDQSPIGRTPRSNPATYTGVFTHIR
                     ELFTRLP"
BASE COUNT          316 a          525 c          531 g          349 t
ORIGIN      
        1 ccggaaggcg cgtgaagagc tcccggatat gggtgaaaac gccggtgtag gtggcggggt
       61 tggacctcgg ggtccgcccg atgggcgact ggtcgatatc gatgacgcgg tcaacggctt
      121 cgaacccgag tataccgtcg gattcgccaa cgcgctcctt tgatctgtac agtttctgtt
      181 tcaatgcggg atagagtgta tcgacgacga gggtgctctt gcctgacccc gatacgccgg
      241 tgacggcggt gaagacaccg aggggtatgg cgacgtcgat gttcttcagg ttgttcgccc
      301 gcgccccctt gaccgtcaga gatccccgtg gctgcctcct gccggccggc ctttcgatag
      361 agcgacgtcc cgacacgtac aggcccgtca ccgattcagg atgcatcttc agttccgcgg
      421 gcgttccctg gaacacgagg tgaccgccct tctcaccggc cccggggccg aggtcgacaa
      481 cgtaatcgga agagacgatc gcgtcgtagt cgtgctcgac gacaataacg gtattgtcga
      541 gatcgcgaag ccgacgcagc gtttgcagga gcctgtcgtt gtccctctgg tggagcccga
      601 tgctgggttc atcgagaacg tagagcaccc ccgtcaggcc cgagcctatc tgcgtggcaa
      661 ggcggatcct ctgcgattca ccggacgaga gggtcgcgga gttgcggctg agactgatat
      721 agtccagacc gacgtcgaga aggaacttga gccgtgatgt gacctccttg agtatcgtcc
      781 gggcgatctc ctgctccttc tcgggaagct cgatcgcgtc aaaaaaggtg gcgcattcct
      841 cgatcgtcat ccgggatacc tcaaagatat tgaggccgcc caccctgacc cagagcatct
      901 ccttcctgag ccgggcaccg ttacactccg gacaggggat gaggttgatg aaccgctcca
      961 gcttctcccg ttcgtagaag tcggcgtcct cataatcccg ggtaagctcc ccgatgaccc
     1021 cctcgaaggg cctccggctg aagctcctgc gcccgccgtg gtcgtggaag aactctatct
     1081 cctcgccttt cgagccatac aggatggcct cccgcacgtc cttcgggagg ctcctgaagg
     1141 gtttcctgat atcgatcctg taatgcttga cgagggcctc gagaaagggg aggaagtgga
     1201 cggggttctt ttcggcccag ggcacgaccg cgccgtcacg gagcgaaagg tcctggttag
     1261 gaacgatcag ggcggggtcg aagtactcct tgacgcccag ccccgtgcac gccgggcacg
     1321 ccccgtaggg attgttgaaa gagaacatcc gcggggctat ctccgggtag ctgatgccgc
     1381 agtcgggaca ggcaaacttc tcgctgaaaa cgcgcgcctg actgccggtc tcgaccttca
     1441 cgaggccgcc tgcccgggaa agggccagct ccacggcctc aaaaagccgc cgttccacgc
     1501 cctcccggat ggtgatgcgg tcgacgacca cctcgatctc atgctttttg ttcttgtcga
     1561 gctcgatatc gtcggcaagg tccatgacgg cgccgtcgat cctgaccttg gtgaaaccgt
     1621 gctgcctgag ctcgtcgatc tccttgcggt attccccttt tcgaccccgg acgatcgggg
     1681 caaggatggt gaggttctct cctttgccga gaccgagaat g
//
LOCUS       DAOQTR010000499         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_93054_length_1715_cov_4.062651, whole genome shotgun sequence.
ACCESSION   DAOQTR010000499 DAOQTR010000000
VERSION     DAOQTR010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93054_length_1715_cov_4.062651"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="tatC"
                     /locus_tag="PKN85_10325"
     CDS             <1..613
                     /gene="tatC"
                     /locus_tag="PKN85_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNT44852.1"
                     /translation="LLQPFKKVMPPGSSFIFTGVTEAFLTYFKIWILTAVTVASPVII
                     HQMWMFVSPGLYEKERRYVYPLIFWGSLLFAGGVVFCYFIVMPNLYRFFVSYASDFVI
                     PMPDLKGYVSLTLKLLVIFGFLFELPLVAYFLAKVGILNHRFLAKKRRYAILGAFILS
                     AIITPPDPISQILVAMPLWGLYELSIVIARLFGKKEKTANEGS"
     gene            600..1616
                     /locus_tag="PKN85_10330"
     CDS             600..1616
                     /locus_tag="PKN85_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNT44853.1"
                     /translation="MKDLDIVILAAGRGERMVSRKPKVMHEIMGKPLVGHVLDAARTL
                     EPARIVVVTGYGRHIVEAYLEDKGVLMAFQEEQKGTAHALASARGHLEGNDVLVLLGD
                     VPLIENFTLVRFLEFCRASASIVFLTTDVDDPSGYGRMVMDGDIIADIREDADATVEE
                     KLIRRINTGICYIPFRDLGLIDLIDTANRKNERYLTDICKVARTQGRHAKGFFHPRAE
                     EVLGVNTLKGLLEANAVMRQRINERHMARGVTFLDADIYVENDVVIGRDTVIAPHCHI
                     AGSTVIGEGVYIGPSSIIRNCRIGDNVTIAGLVFMEGVEAKEGVNIGALSRFKQDMVL
                     ERKT"
BASE COUNT          355 a          499 c          504 g          357 t
ORIGIN      
        1 tctcctgcag cccttcaaga aggtcatgcc gccggggagc agtttcatat tcacgggggt
       61 caccgaggcg tttctcacct atttcaaaat atggatactc acggccgtca ccgtggcatc
      121 gcccgtcatc attcaccaga tgtggatgtt cgtctctccc ggcctctacg agaaggaacg
      181 gagatacgtc tatcccctca tcttctgggg cagtctcctt ttcgccggcg gagtggtctt
      241 ctgttacttc atcgtcatgc ccaatctcta caggtttttc gtcagctacg cctccgactt
      301 cgttatcccc atgcctgacc tcaaggggta tgttagcctc acattgaagc ttctcgtcat
      361 cttcgggttc ctcttcgaac tcccccttgt ggcctatttt ctggcgaagg tggggatact
      421 gaaccatcgg ttcctggcaa agaagcggcg ctacgcgatc ctgggagcgt tcatcctgag
      481 cgccatcata acacccccgg acccgatcag ccagatactc gtcgccatgc ccctctgggg
      541 cctctacgaa ttgagcattg tcatcgcccg tttatttgga aagaaggaga agactgccaa
      601 tgaaggatct tgatatcgtc attcttgccg cgggcagggg agaacggatg gtctcccgca
      661 agccaaaggt catgcatgag atcatgggga aacccctcgt gggacacgtc ctcgacgccg
      721 cgcgtactct cgaacccgcc cgcatcgtcg tcgtcaccgg gtatggacgt cacatcgtcg
      781 aggcctatct cgaggacaag ggagtcctga tggcgttcca ggaagagcaa aagggaaccg
      841 cccacgccct ggcatccgcg cgggggcacc tggaggggaa cgacgtcctc gttctcctcg
      901 gtgacgttcc cctcatcgag aatttcacgc tcgtccggtt ccttgagttc tgccgggcct
      961 cggcttcgat cgtcttcctg accacggatg tcgacgatcc ttccggctac ggccggatgg
     1021 tgatggatgg cgacatcata gcggatatcc gggaggacgc cgatgcgacg gtcgaggaga
     1081 aactgatacg caggatcaac acgggcatct gttacatacc tttccgggac ctcgggttga
     1141 tcgacctcat cgataccgcg aacaggaaaa atgagcgcta cctgacggac atctgcaagg
     1201 tagccaggac ccagggccgc catgcgaagg gcttcttcca tccccgcgcc gaggaggtcc
     1261 tcggcgtcaa taccctgaag ggcctccttg aggcgaacgc ggtgatgagg cagcggatca
     1321 acgagcggca catggcccgc ggcgtgacgt tcctcgacgc cgacatctac gtggagaacg
     1381 acgtggtcat aggccgcgac accgtcatcg caccccactg ccacatcgcg gggagcacgg
     1441 tcatcgggga aggtgtctac atcgggccct catcgatcat caggaactgc aggataggcg
     1501 acaacgtgac catagcgggc ctggtcttca tggagggggt ggaggcaaag gagggtgtca
     1561 acataggcgc tctctcgcgg ttcaagcagg acatggtcct ggaacgaaag acatgagcgg
     1621 gataaggagg agacagcggt gtgcggtatc gttgggtatc agggtaagga aagggcacgg
     1681 gacattctca tcgatgcctt gaggcgcttg gagta
//
LOCUS       DAOQTR010000500         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_93667_length_1708_cov_4.148820, whole genome shotgun sequence.
ACCESSION   DAOQTR010000500 DAOQTR010000000
VERSION     DAOQTR010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93667_length_1708_cov_4.148820"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..522
                     /locus_tag="PKN85_10335"
     CDS             139..522
                     /locus_tag="PKN85_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNT44854.1"
                     /translation="MRKEVRIALGLAVVVVFVLTLLAGAFAQKKAPESMMLKLEGAKF
                     PPVAFSHPQHTEKAKIDCVECHHKDKNPKEPGGCMPCHDLKDVKNGAIPIKDAYHKNC
                     IECHKQSSAKGVKAPTKCNDCHKKQ"
     gene            500..>1708
                     /locus_tag="PKN85_10340"
     CDS             500..>1708
                     /locus_tag="PKN85_10340"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNT44855.1"
                     /translation="MTATKSNSGTETPPASRAASLSRSLASVRLGIVVLSLIAATSIL
                     GSVIKQGGTEEEYLSLYSEKAYHFIKLFGFDDAYHSPWFYFLLALFALNLIACTVRRI
                     RGLTGERGPGAKNAPDVKALAAAGSGFSAPVDTLGEISRRLSASSYKRKQLSERTTLY
                     EKGSLARYGVLVIHASVLIVLLGGLIGLVAGDRGFLVLRVGETAEAAVSRERARLPVP
                     LGFTVRCKDFNVTFYPGGQPKEYASDIEILDGAGKVLKEGRIKVNEPLSYNGVRLYQS
                     SYGTSNSYTFRVDGREVVLAEQEVAKAGRTAFMVVRYAPQVHDFGPGVMVAYMDGEEP
                     KTLWLLKDVERMRTRVIQGSRISLEGITSGYYTGLEMSRDPGVPVVLAGFGLMLAGLY
                     INFFTFHRRIY"
BASE COUNT          362 a          490 c          531 g          325 t
ORIGIN      
        1 aaccttttct tctgggtata ccccagggtg gtaagtatgt ccatgcccaa ctactacccc
       61 cggcccgctt tgcttgactt atctcccaaa cttgcgataa cattctgagt atttgaaaac
      121 ccgatgaaga ggtgtggcat gagaaaggaa gtaaggatcg cactcggact ggcagtggtc
      181 gttgtcttcg tcctcacgtt gctcgcgggt gcttttgccc agaaaaaggc cccggaatcg
      241 atgatgctga agctcgaggg ggcgaagttc cctcccgtgg ctttctccca tcctcagcat
      301 accgaaaagg caaagataga ctgtgtcgaa tgccaccaca aggacaagaa cccgaaggag
      361 cccgggggat gcatgccctg ccacgacctc aaggacgtga agaacggcgc gataccgatc
      421 aaggacgcat accacaagaa ctgcatagaa tgtcacaaac agtcatcggc gaaaggggta
      481 aaggcgccga cgaagtgtaa tgactgccac aaaaagcaat agcggaacgg aaaccccgcc
      541 ggcctcacgg gcagcctccc tctcccgatc cctcgcatcg gtgcgcctcg gtatcgtcgt
      601 gttgtcgctc atcgcggcga catccattct cgggagcgtg atcaagcagg ggggcacgga
      661 ggaggagtac ctctcacttt acagcgagaa ggcctatcat ttcataaagc tcttcgggtt
      721 cgacgacgcc taccattcgc cgtggttcta ttttctcctg gccctttttg ccctcaacct
      781 catcgcgtgc accgtgagac gcatacgggg tctcacgggg gaaagagggc cgggggcaaa
      841 gaacgcgccg gacgtgaagg ccctcgcggc cgcggggtcg ggctttagcg cccccgtgga
      901 cactctcggc gagatctccc gccgcctcag cgcctcgtcg tataagagga agcagctgtc
      961 ggaaaggaca acgctttacg agaaagggtc tctcgcgcga tacggggtcc tcgtcatcca
     1021 cgcaagcgtc ctcatcgtgc ttctgggcgg gctcatcggg ctcgttgcgg gcgacagggg
     1081 tttccttgtg ctgcgcgtgg gcgagacggc cgaggccgcc gtctcgcgcg agagggcgcg
     1141 gcttcccgta ccgctcggct tcaccgtgag gtgcaaggat ttcaacgtca ccttctatcc
     1201 cggggggcag ccgaaagagt acgcaagcga catcgagatc ctcgacggcg cgggcaaggt
     1261 cctgaaggag ggcaggatca aggtgaacga gcccctgtcc tacaatgggg tcaggttgta
     1321 ccagtcgagc tacgggacat cgaacagcta caccttccgc gtggacggga gggaggtcgt
     1381 cctcgcggag caggaggtgg cgaaggcggg caggaccgct ttcatggttg tgaggtacgc
     1441 cccgcaggtg cacgatttcg gccccggggt gatggtcgcc tacatggatg gggaagagcc
     1501 gaagacgctc tggctcctga aggacgtcga aaggatgaga acccgggtga tacagggatc
     1561 acggatctct ctcgaaggga taacgagcgg atactacacg ggtctcgaga tgtcccgcga
     1621 tcccggcgtg cccgtggtgc tcgcggggtt cggcctcatg ctggccgggc tctacataaa
     1681 tttcttcact ttccaccgga ggatatat
//
LOCUS       DAOQTR010000501         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_93888_length_1706_cov_3.133858, whole genome shotgun sequence.
ACCESSION   DAOQTR010000501 DAOQTR010000000
VERSION     DAOQTR010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93888_length_1706_cov_3.133858"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKN85_10345"
     CDS             <1..730
                     /locus_tag="PKN85_10345"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNT44856.1"
                     /translation="FYGPENTIITISGRVNHETCVEEIGRRFFGIPAGRADGRELVPP
                     RATTGIDIYEKDLEHIYLCIGTDGVSQVDRRRYVLYVLNAIMGGSMSSHLFQEIREQR
                     GLVYNIYSYVNCYHDAGTFGISTSTSQESMMEVLTLIREEIARIRDRGITDAELSFSK
                     EHIKGNLFIALEGSETRMGRLAKNEIYFGTYIPLKETLREIDRITKKDVAAIAREIFD
                     STKVVSLTVLGNVDREKVEKVWRE"
     gene            718..1158
                     /gene="dut"
                     /locus_tag="PKN85_10350"
     CDS             718..1158
                     /gene="dut"
                     /locus_tag="PKN85_10350"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNT44857.1"
                     /translation="MERIEVFIKMRDGATVPAYATDRASGVDLCALVDGPVVLGPMER
                     ALIPTGIFIDIPEGFEAEVRPRSGIAHKHGVTVLNTPGTIDSDYRGEVKVILINLGGD
                     PFTIRSGDRIAQMVFKRLARVDFRPVASLADSCRGEGGFGSTGV"
     gene            1173..>1706
                     /locus_tag="PKN85_10355"
     CDS             1173..>1706
                     /locus_tag="PKN85_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNT44858.1"
                     /translation="MERLYHHLDSYVRHLVSERGASPHTVEAYNRDILTFIRYCEDIA
                     CPEPERRHVEAFIGYMRENGRSTRSIVRSLSALRGFFNYLLLDGRIKVSPLDDVDTPR
                     FRPPIPRVLSEDEMGELIHLPEGGRMALRDRTMLELLYATGLRVSELIRLKKGDINLD
                     GGFVVAMGKRSKERVVPL"
BASE COUNT          378 a          468 c          542 g          318 t
ORIGIN      
        1 gttctacggg ccggagaaca cgataatcac catcagcggc agagtgaacc acgagacctg
       61 cgtggaggag ataggacggc gttttttcgg catcccggca ggaagggccg acggcaggga
      121 gctcgtcccg ccgcgcgcga cgacggggat agacatatac gaaaaggacc tggagcacat
      181 ctacctgtgc ataggcacgg acggtgtcag tcaggtcgac aggcgcaggt acgtgctcta
      241 cgtgctgaac gcgataatgg gcggcagcat gagctcccac cttttccagg agatacggga
      301 gcagaggggc ctcgtgtaca atatctactc ctacgtcaat tgctaccatg acgcgggtac
      361 gttcgggata tccacgtcca cgtcgcagga atccatgatg gaggtgctca ctctcatcag
      421 ggaggagata gcgcgcatcc gggacagggg cataaccgat gccgagcttt ccttttcgaa
      481 ggagcacata aagggcaacc tcttcatcgc ccttgagggc tcggagacac ggatgggccg
      541 cctggcgaag aacgagatat acttcgggac atacataccc ctcaaagaga ccctccggga
      601 gatagacagg atcaccaaga aggacgtggc tgccattgcc cgcgagatat tcgacagcac
      661 gaaggtggtg tcgctgaccg tgctcggcaa cgtggaccgc gagaaggtcg aaaaggtatg
      721 gagagaatag aggtcttcat caaaatgcgc gacggtgcga cggtgccggc gtacgctacg
      781 gaccgtgcct ccggggtcga tctgtgcgcc ctggtcgatg gtcccgttgt ccttggaccc
      841 atggaacggg cccttatccc gacgggcatc ttcatagaca tacccgaagg tttcgaagcg
      901 gaggtgcgtc cccgcagcgg catcgcccac aagcacgggg tgacggtgct caacacaccg
      961 ggcaccatcg attccgatta ccggggcgag gtcaaggtga tcctcatcaa cctgggcgga
     1021 gaccccttca cgataagaag cggagatagg atcgcccaga tggtcttcaa aaggctggcc
     1081 agagtggatt tccggcctgt cgcgagtctc gccgattcgt gccgcggcga gggaggtttt
     1141 ggttccacgg gagtataggg gagatacggc aggtggaacg tctctatcac catctcgaca
     1201 gttacgtccg tcacctcgtc tcagaacgcg gggcttcgcc gcacacggtc gaggcctaca
     1261 accgggatat cctgacattc atacgatatt gtgaggacat agcctgcccg gagccggaaa
     1321 ggcgccatgt ggaggccttt atcgggtaca tgcgcgaaaa tggcaggtcg acgcggagca
     1381 tcgtgaggag tctttccgcc ttgagaggtt tcttcaatta tcttcttctc gacggcagga
     1441 tcaaggtcag tcctctcgac gatgtggata cgccaagatt ccggccgccc attccccggg
     1501 tgctttccga ggacgagatg ggtgaactca tccacctgcc cgaggggggg aggatggccc
     1561 tgagagaccg gacgatgctc gagctcctct acgcgacggg tctgagggtc tcggagctca
     1621 tacggctcaa gaagggagat atcaacctgg atggcggctt cgtcgtcgcc atgggaaagc
     1681 gttccaagga acgggtcgtg ccgctt
//
LOCUS       DAOQTR010000502         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_94092_length_1704_cov_2.755003, whole genome shotgun sequence.
ACCESSION   DAOQTR010000502 DAOQTR010000000
VERSION     DAOQTR010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94092_length_1704_cov_2.755003"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PKN85_10360"
     CDS             complement(<1..1317)
                     /locus_tag="PKN85_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNT44859.1"
                     /translation="MAIDPIKKLTILSPMHSSKRLLRTVSALGIVDVTDAGEGLEETA
                     PGMKIKGVSTEDIDDVLHKIDAILNLLKAYAPEEQGFFQGLTPVPQIIDKQELKEATD
                     NYDLAARSRQANELDEVLRRSERAKGDIENRLTALLPLCDLSFAMEDFHRPRRIRLVF
                     GHLPRKNLEHLSRPVSPWTGIAWELIEEGPDTCGTYPSGFTPAPGGKRAANQDNVRIL
                     IAVLADDVDEVKDGLSRLSFIETELPDFSGTISDRVRTLQEDLLQLSATIEETVTEVR
                     ALASDRRTLITLKAFWTAARNERLALTKTLGGRWVHLLTGYIRARDVERLQGAFAKEF
                     PDSVIILDDPSEDENVPVSLSLPKVFRPLSLLVEMFGLPPYRGFDPTPYLHVNFYLFF
                     GICFSDVGYGLMLIATSLYLIKRTKQYQGVADFGRILLGGGISTVIF"
     gene            complement(1322..1633)
                     /locus_tag="PKN85_10365"
     CDS             complement(1322..1633)
                     /locus_tag="PKN85_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44860.1"
                     /translation="MASLLDNIIALEQEAEGIIEAAHTRSKTILASADDELTRYRGEL
                     AAELEARLAEFRADVEKRFEESLSQATAQHARRLRAIRELPQEFSAREVNRILDRFNN
                     W"
BASE COUNT          313 a          470 c          544 g          377 t
ORIGIN      
        1 gaagatgacg gtacttatgc cgccgccgag aaggatacgc ccgaaatccg ccacgccctg
       61 gtattgtttt gtccttttaa tgagatagag tgaggtcgcg atgagcatga ggccataccc
      121 gacatcggaa aagcatatgc cgaagaagag atagaagttc acgtgcagat acggcgtggg
      181 gtcaaagccg cggtacggcg gcaggccgaa catctccacc agcagggaga gcggccggaa
      241 gaccttcgga agggagaggc taaccggtac gttctcgtct tcggaagggt cgtcgaggat
      301 gataacagag tcgggaaact ctttggcgaa ggcgccttgc agacgctcga catcccgggc
      361 ccgtatgtac ccggtgagaa gatggaccca tctcccgccg agggtctttg tcaacgccag
      421 tcgttcgttt ctggcagccg tccagaaagc cttgagggta atgagggtac gtcggtcgga
      481 ggcaagcgcc ctgacctccg tgacggtctc ctcgatggtg gcggacaact ggagcaggtc
      541 ttcctggagg gtcctgacac ggtcgcttat cgtccccgag aagtcgggga gctccgtttc
      601 gatgaaggaa agccgggaca gtccgtcctt cacctcgtcc acgtcatcgg cgaggacggc
      661 aatgagtatc ctgacgttgt cctgattcgc cgctcttttc cccccggggg cgggtgtgaa
      721 cccggacggg tatgtcccgc aggtgtcggg cccctcctcg atcaattccc aggcgatgcc
      781 ggtccaggga gataccggcc ttgagaggtg ctccaggttc ttgcggggca ggtggccaaa
      841 gaccaggcga atccggcggg gacggtgaaa atcctccatt gcgaacgaga ggtcgcacag
      901 gggcaagagc gcggtcaggc ggttctcgat gtctcctttt gcgcgctcgc tgcgcctgag
      961 tacctcgtcg agttcgtttg cctgccggga gcgggcggcg agatcgtagt tgtccgtcgc
     1021 ttctttgagc tcctgcttgt cgattatctg ggggaccggc gtcagaccct ggaagaaccc
     1081 ctgttcttcg ggtgcgtagg ccttcagaag attgaggatg gcgtctatct tgtgaagtac
     1141 gtcgtcgatg tcttctgtgg agacaccctt gatcttcatg ccgggggctg tttcttcgag
     1201 gccttcaccg gcatcggtaa cgtcgacgat gccaagggcg ctgacggtgc gcagaaggcg
     1261 cttgctgcta tgcatggggc ttaagatcgt cagcttcttg atggggtcta tcgccattcg
     1321 tctaccagtt gttgaacctg tccagtatcc tgttgacctc acgcgctgag aactcctggg
     1381 gcagttcacg gatcgcccgc agtctccggg cgtgctgcgc ggttgcctga ctcagagact
     1441 cctcgaatct tttttcgacg tcggccctga actctgcgag gcgggcctcg agctcggccg
     1501 cgagttcccc gcgataccgc gtgagttcgt catcggccga cgcgaggatg gttttggacc
     1561 gggtgtgggc agcttcgatg atgccctccg cctcctgttc cagcgctatt atgttgtcaa
     1621 gcagggatgc catatcgttg tcccttctca tcattcgaaa agttgtgcga tatcagccga
     1681 aggacaggtc cggctggatg aata
//
LOCUS       DAOQTR010000503         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_94678_length_1698_cov_2.706634, whole genome shotgun sequence.
ACCESSION   DAOQTR010000503 DAOQTR010000000
VERSION     DAOQTR010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94678_length_1698_cov_2.706634"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKN85_10370"
     CDS             <1..570
                     /locus_tag="PKN85_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT44861.1"
                     /translation="LKSPGVRVHVSNPHNTTVIPGTPCIIMSNHRSHYDIPLIFTSLP
                     GSIRMLTKKELFKVPIWGRGLKAAEFLSIDRHDHAQAIKDLEYAKEQMKSGIVLWIAP
                     EGTRSRTGALGEFKKGGFMVAIQTGATIIPVGIRDSEKILKPGTWDFCLNQDVHITIG
                     RPFDASTYTLETRDRLMEDVRRSIQELAQ"
     gene            complement(638..1384)
                     /locus_tag="PKN85_10375"
     CDS             complement(638..1384)
                     /locus_tag="PKN85_10375"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNT44862.1"
                     /translation="MDWAAIENLGYDVRVSTPYTIVHKRSLPLLPELIAGGPVLGTDT
                     SVTGRTSIRIIEPDLMVRTLTHGGAFRNIFKARFLTVDRSLRELDVSAHLISHRILTP
                     EIVGLRIRKQGFFHFIEVITRMIPDSIDLLAYLEKSPDDGLNVIRDTGALIRAVHDAR
                     VYHADLHVKNIVLDRGKRPWIIDLDSAYRFIDLPFILKYKNTRRFIHSLKKWDRKERI
                     RLPQNWKQAFFSGYASSAPGSSAPGSGLDM"
     gene            complement(1388..>1698)
                     /locus_tag="PKN85_10380"
     CDS             complement(1388..>1698)
                     /locus_tag="PKN85_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNT44863.1"
                     /translation="TAETQRRREKQKEYNRLHGITPTTIRKNIRDILASIYERDYADM
                     TAVVGTSLGNVEVGKLKETIAELEKKMHQAADDLRFEDAASLRDEIKRLTRIALVTEG
                     "
BASE COUNT          349 a          476 c          499 g          374 t
ORIGIN      
        1 ctcaagagcc ccggggtgcg ggtgcatgtc tccaaccctc acaataccac tgtaattccc
       61 ggcaccccct gcatcatcat gtcgaatcac cggagccact acgacattcc gctgatcttc
      121 acctcgctcc cgggaagcat tcgcatgctg acgaaaaagg agctgttcaa ggtgccgatc
      181 tgggggagag ggctgaaagc ggccgaattt ctctccatcg accgccacga ccacgcgcag
      241 gcgatcaaag atctggagta tgcaaaggag cagatgaaga gcgggatcgt gctctggata
      301 gcgccggaag gcaccaggtc ccgaaccggc gccctgggcg agttcaagaa gggcgggttc
      361 atggtggcca tccagaccgg ggccacgatc atcccggtgg gaatcagaga ctcggaaaaa
      421 atcctcaagc ccggcacctg ggacttttgc ctgaaccagg acgtgcacat cacgatcggc
      481 cggccattcg acgcctccac ttacaccctg gaaaccagag accgtctcat ggaagacgtg
      541 aggagatcga tccaggagct cgcgcaatag cagtcagcgg tcaccgttgg ggtccagttg
      601 gggtcaggtc ttgacatgtg acaagtcttc agacttgtca catgtcaaga cctgaccccg
      661 gggctgatga ccccggggct gatgacgcgt agccgctgaa aaaggcttgt ttccagtttt
      721 ggggcagtcg gatgcgctct tttctgtccc acttcttgag agagtgtatg aagcgcctgg
      781 tatttttgta tttcaggata aagggtaggt cgatgaaccg gtatgcactg tccaggtcga
      841 tgatccacgg ccttttcccg cggtctagga caatgttctt cacgtgcagg tcagcgtggt
      901 agacgcgtgc gtcgtggacg gccctaatga gggctccggt atctctgatg acattgagcc
      961 cgtcatcggg gcttttctcc agataggcga gcagatcgat ggaatccggg atcatccgcg
     1021 tgatcacctc gatgaaatgg aagaagccct gctttcttat cctcagcccc acgatctcag
     1081 gggtgaggat gcggtgtgaa atgagatggg cgctgacgtc gagctcgcgg agactcctgt
     1141 cgacggtgag aaaccgcgcc ttgaaaatgt tcctgaacgc accgccatgg gtcagagttc
     1201 gcaccatcag gtcgggctcg atgatccgta tggatgtcct gcccgtgacg gatgtgtctg
     1261 tgccgagaac aggaccgcct gcgatcagct ccggcagaag gggcagggag cgcttgtgga
     1321 cgatggtgta cggggtcgat acccgcacgt cgtatcccag attttcgatg gctgcccagt
     1381 ccacactcta accttcggtc accagggcga tcctggtgag gcgtttgatc tcgtcgcgca
     1441 aagacgctgc atcttcgaac cgcagatcgt cggccgcctg gtgcatcttt ttttcgagct
     1501 cggctatggt ctccttcagc tttccgacct cgacattgcc cagcgaagtg cccacaaccg
     1561 ctgtcatatc tgcataatcc ctctcgtaaa ttgatgcaag gatatcccgt atgtttttcc
     1621 tgatagtggt tggggtgatg ccgtgaaggc gattgtactc cttctgcttc tcccgcctgc
     1681 gctgcgtctc ggcagtgg
//
LOCUS       DAOQTR010000504         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_94835_length_1696_cov_3.762949, whole genome shotgun sequence.
ACCESSION   DAOQTR010000504 DAOQTR010000000
VERSION     DAOQTR010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94835_length_1696_cov_3.762949"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKN85_10385"
     CDS             <1..448
                     /locus_tag="PKN85_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44864.1"
                     /translation="AAIMLLALAGAAMAVDIDTLDRVSVLMPKSEVIAILGPPDDVLD
                     VGAGLKAEVYKVEGIEPMMGTGCIYDGDRLAGQSFMFAGEMTAGAAERLKKHGFALLE
                     EKNAAYRLLGNDDDTGRVFMVHVFSEMGMTIVMTFEKEFYDRHNKQ"
     gene            492..1607
                     /locus_tag="PKN85_10390"
     CDS             492..1607
                     /locus_tag="PKN85_10390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT44865.1"
                     /translation="MKRSPAFIIPFAVITAILFLFLAADCPAAAPELTPGKIDEVVTK
                     IMKEKNIPGLSLAISTPGRTIERSYGIANMEYNIPVEKDTIFEIGSITKTFTAIGILI
                     LQQEGKLSVTDRITKYFPEYPQWHDITLKHLLQHTSGIREMTEIEPFKSNQAKDWNPR
                     EVIARMAEEPLDFQPGQNAAYSNIGCIILGVVIEKVTGVSYNDFLNDRILKPLGMART
                     MFGSTSAIIPKRASGYAFAGKHVNAPFASLVLPHASGGMTSTASDLVKLAKVFTAKAL
                     LTEKSVKEMFAPARLNNGTEFLLPGPVINMTFGYSLDSVRGKKVIPAKTGGISGFNSY
                     FAYFPESQAMVALTANLDNSLESLVIITDALLGLKGR"
BASE COUNT          387 a          527 c          479 g          303 t
ORIGIN      
        1 cgcggcgatc atgctcctcg cgcttgccgg ggctgcaatg gccgtagaca tagacaccct
       61 cgacagggtc agcgtcctca tgccgaaatc cgaggtcatt gccatactcg gtcctcccga
      121 cgatgtgctt gacgtcggcg cgggcctcaa ggccgaggtg tacaaagtgg agggcattga
      181 gccgatgatg ggaacaggct gcatctatga cggcgaccgt cttgccggcc agtccttcat
      241 gttcgccggg gagatgaccg ccggggccgc cgagagattg aaaaagcacg gcttcgccct
      301 cctcgaggag aagaacgccg cctatcggct tctcgggaac gatgacgaca cgggcagggt
      361 cttcatggtc catgtcttca gcgagatggg aatgacgatc gtcatgactt tcgagaagga
      421 gttctacgac aggcacaaca agcagtgacg gccttaccgg aagaaagatg caggccccag
      481 gaggaacgat tatgaagaga agcccggcat tcatcatacc cttcgcggtg atcaccgcca
      541 tcctttttct gttcctcgcc gccgactgcc ccgcggcggc tcccgagctc accccgggga
      601 agatcgacga ggtcgtcacg aagatcatga aggagaagaa cataccgggg ctgtcccttg
      661 ccatatccac gcccggaagg acgatcgaga gaagctacgg catcgccaac atggaataca
      721 acatccccgt ggagaaggac accatcttcg agataggctc catcaccaag accttcacgg
      781 ccatagggat actgatcctc cagcaggagg gaaagctcag cgtcaccgac aggataacga
      841 agtacttccc cgagtacccc cagtggcacg acatcaccct caaacacctc ctccagcata
      901 cctcgggcat acgggaaatg accgagatcg agcccttcaa gagcaaccag gcgaaggact
      961 ggaacccccg ggaggtcatc gcccgcatgg ctgaagagcc cctcgatttc cagcccggac
     1021 agaacgcggc ttacagcaac atagggtgca tcatcctggg ggtggtcatc gagaaggtga
     1081 cgggtgtttc ctacaacgac ttcctgaacg acaggatctt gaagcccctg ggaatggccc
     1141 gcacgatgtt cggcagcacg agcgccatca tcccgaaacg ggcgtcgggc tacgccttcg
     1201 ccggcaagca cgtgaacgcc ccctttgcga gcctcgtcct gccccatgcg agcggcggca
     1261 tgacctccac cgcctcagac ctcgtcaagc tcgcaaaggt cttcacggca aaggcccttc
     1321 tcaccgaaaa gtccgtgaag gagatgttcg cccccgcccg ccttaacaac gggacggagt
     1381 tcctgcttcc cggacccgtc atcaacatga ccttcggcta cagccttgac tcggtgaggg
     1441 gcaagaaggt gatccccgcc aagacaggcg ggatatcggg gttcaactcc tacttcgcct
     1501 acttccccga atctcaggcc atggtggccc tgacggcaaa cctcgataac tcgcttgaaa
     1561 gccttgtcat catcaccgac gccctcctcg ggctgaaggg caggtagggg ccggtatacc
     1621 tgcgtcttcc gtcgtgatat attgaagacc ttgagagcga catgggactt gtgcgcatac
     1681 atgatgtttc cttgag
//
LOCUS       DAOQTR010000505         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_94857_length_1696_cov_2.572212, whole genome shotgun sequence.
ACCESSION   DAOQTR010000505 DAOQTR010000000
VERSION     DAOQTR010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94857_length_1696_cov_2.572212"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PKN85_10395"
     CDS             <1..584
                     /locus_tag="PKN85_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT44866.1"
                     /translation="FLEYFCSLKAIFTPDYIGFPRPFEVSAWNFSLGGIDFSFTNVQV
                     LIFAITFFTLALLYLLVYRTKQGTAMRAVSHDQETAALMGINIDHTVSLTFMVGAALA
                     GVGGILYGIAYPQINVFMGIMPGIKSFIAAVLGGIGIIHGAVVGGLIVGVSEVIVSAF
                     LSSTYRDGVIFVILLIVLLVKPSGLFGKRIDKV"
     gene            581..1525
                     /locus_tag="PKN85_10400"
     CDS             581..1525
                     /locus_tag="PKN85_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT44867.1"
                     /translation="MKFFLESRRLILLAAGIYIVLKLLFSFGIVSEYIQQITIYVFIV
                     VLAATGLNVIYGYTGQFSLGHAAFYGIGAYTSAYLVKTLGLDSVFALPATLLAGGIMA
                     GLVACVIGIPILRLRDDFLGIATLGFGILVRVFFDNSDRISETLGGSRGFTGIAKLTN
                     LEITFVVTVFLLIVTRNLISSRYGLFWRCIKDDETASASVGIDTTRMKLMAFSYGCFL
                     AGVAGALYAHLYTFLHPSNFDFLRSIDFLIIVILGGMGNIAGTIWAGVLWVGFIEGLR
                     IVLPSNVLDLRWVFIPLLLIMLMMWRPYGLMSKKKGNG"
     gene            1564..>1696
                     /locus_tag="PKN85_10405"
     CDS             1564..>1696
                     /locus_tag="PKN85_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT44868.1"
                     /translation="MLEVERLEKSFGGVRAVDGFSFHVDRGETLGIIGPNGAGKTTVF
                     "
BASE COUNT          298 a          513 c          479 g          406 t
ORIGIN      
        1 tcttccttga atacttctgc agcctgaagg ccatcttcac accggactac atcggctttc
       61 cccgcccctt cgaggtgtcg gcctggaact tttcgctcgg cgggatcgat ttctccttca
      121 caaacgtgca ggtgctcatc ttcgccatca cgttcttcac tctcgcgctc ctgtaccttc
      181 tcgtttacag aacaaaacag ggcacggcca tgcgcgccgt gtcccatgac caggagacgg
      241 cggcgctcat ggggatcaac atcgaccaca ccgtttccct cactttcatg gtgggagcgg
      301 ccctcgccgg cgttgggggc attctttacg gaatagccta tccccagatc aatgtcttca
      361 tggggatcat gccggggata aagtccttca tcgcagccgt gcttgggggc atcggcatca
      421 tccacggggc cgtcgtcgga ggcctgatcg tcggtgtgtc cgaggtcatc gtgtcggcct
      481 tcctctcctc gacgtatcgg gacggggtca tcttcgtgat ccttctcatc gtccttctcg
      541 tcaagccgag cggcctcttc ggcaaaagga tcgacaaggt atgaagttct tcctggagtc
      601 caggagactg atccttcttg ccgcgggcat ctatatcgtc cttaagctcc tcttcagctt
      661 cggcatcgtc tcagagtaca tccagcagat aaccatctat gtcttcatcg tggtgctcgc
      721 ggcgacgggc ctcaacgtca tctacggcta taccggtcag ttctccctgg gccacgcggc
      781 gttctacggg atcggggcgt acacctcggc ctatctcgtc aagaccctgg ggctcgacag
      841 cgtcttcgcc ctccccgcca cactcctcgc cgggggcatc atggccgggc tcgtcgcctg
      901 cgtcatcggg ataccgatcc tgaggctccg ggacgacttc ctcgggatcg ccaccctggg
      961 tttcggtata ctggtgcggg tcttcttcga caattccgac aggatctcgg agacactggg
     1021 gggttcacgg ggcttcacgg gcatcgcaaa actcaccaac ctggagatca ccttcgtcgt
     1081 caccgtcttc cttctcatcg tcacgaggaa cctcatctct tcgcggtacg ggctcttctg
     1141 gcggtgcata aaggatgacg agacggcgag cgcctcggtc ggcatcgaca ccacgcgcat
     1201 gaagctcatg gcgttctctt acggctgctt tctcgccggc gtcgccgggg ccctctatgc
     1261 ccatctctac acctttctcc acccctccaa tttcgatttc ctcagatcca tcgatttcct
     1321 gatcatcgtc atcctcgggg gcatggggaa catagcgggc acgatctggg cgggcgtcct
     1381 ctgggtgggt ttcatagaag gcctgaggat agtcctaccg tcgaacgtgc tcgatctcag
     1441 gtgggtcttc attcctcttc ttctcatcat gctgatgatg tggaggcctt acgggctaat
     1501 gagcaaaaag aagggtaatg gttgatgggt cacgggtcat ggggagaaga cgggatgacg
     1561 gcaatgcttg aggtggaaag attggagaag agcttcggcg gggtccgggc tgtggacggg
     1621 ttctcctttc atgtcgacag aggggaaact ctgggtatca tcggtcccaa cggggcggga
     1681 aagacgacgg tcttca
//
LOCUS       DAOQTR010000506         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_95710_length_1687_cov_2.367034, whole genome shotgun sequence.
ACCESSION   DAOQTR010000506 DAOQTR010000000
VERSION     DAOQTR010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95710_length_1687_cov_2.367034"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..662)
                     /locus_tag="PKN85_10410"
     CDS             complement(108..662)
                     /locus_tag="PKN85_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNT44869.1"
                     /translation="MEDPHQRDSRSTYPDRIFRFCPRCGSGGLIAGGGKPFVCGGCGF
                     EYYINAAAAVVALIEDSENRLLLTRRARDPKIGTLDLPGGFVNVHETAEHALIREVRE
                     ELHLELAGFSYFGSFPNTYPYGGITYFTLDLAFVCSVPDLSTLQAGDDAASYIFIPLQ
                     DIRLKDIGLDSIQKIVSLYIDSRS"
     gene            complement(735..1391)
                     /locus_tag="PKN85_10415"
     CDS             complement(735..1391)
                     /locus_tag="PKN85_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNT44870.1"
                     /translation="MIRYVFAVMLGVLFCSLPALGAPPGQGWELAKQGEGIEVYTRPV
                     EGMDAKEFLGVADVDAPVDVVLEVFRDVPSFPGWYGFCRDIQVVRDFSDRHKIVYFVL
                     ATQWPTKDRDMVIDVMIEEGGDKEKYVLSMKALKEELVPRSRKYVRMTHMIGRYTLTE
                     IDTDRTHVTYTVQSDPAGYIPAALSNMLAKDQPYLTLKGLREMVKEDVYYERAGLKRR
                     "
BASE COUNT          365 a          511 c          455 g          356 t
ORIGIN      
        1 ggggtcaggt cttgacatat gacatttcca agtaaatgcc aggattgaca agatagggaa
       61 atgtcatatg tcaagacctg accccatcca agatgagtct ttcatcccta cgaacgggaa
      121 tcaatataga gagaaacgat cttttgtatg gaatcgaggc ctatgtcctt aagccggatg
      181 tcctgaaggg gaatgaagat gtacgacgcc gcgtcgtccc ccgcctgtag ggtcgacagg
      241 tcgggcaccg agcagacgaa ggcgagatcc agcgtgaagt aggtgatgcc tccgtaggga
      301 taggtgttgg ggaacgagcc gaagtaggag aagccggcca gctcgagatg cagctcctcc
      361 ctgacctccc gtatcagggc gtgctcggcc gtctcgtgta cgttcacgaa cccgccgggc
      421 aggtccaggg tcccgatctt cggatcgcgg gccctccgcg tcaggagcag acgattctcg
      481 gagtcctcga tcagggcgac aacggcggcc gccgcgttga tataatattc gaacccgcat
      541 ccgccgcaca cgaacggttt gccgcccccg gcgatcagcc cgcccgaacc gcaccggggg
      601 cagaacctga agatgcgatc cggataggtg cttctcgaat ctctctggtg cggatcttcc
      661 atcggcatcc ctttccgaga atggatgttc ctcacatcgg gcaactgggc cgctcatccc
      721 tttccgtcac gacgtcaccg cctcttcaga cccgccctct cataatacac gtcctccttg
      781 accatctccc tgagcccctt gagagtgaga tagggctggt ccttggcgag catgttggag
      841 agggccgcgg gaatgtatcc ggcagggtct gactggactg tataggtgac atgggttctg
      901 tcggtgtcga tttcggtaag ggtatagcgg ccgatcatgt gggtcatcct gacatacttc
      961 ctgcttcggg gcacaagctc ttccttcagc gccttcatgg acaacacgta cttctccttg
     1021 tccccgccct cttcgatcat cacatcgatc accatgtcac ggtctttcgt cggccactgg
     1081 gtcgcgagga cgaaatagac gatcttgtgc ctgtcggaga agtcccgaac cacctgtatg
     1141 tcgcggcaga agccgtacca cccggggaac gaggggacgt ctctgaacac ctccagaacc
     1201 acatcaaccg gtgcatccac gtcggcaaca cccaggaatt cctttgcatc cattccctct
     1261 accggcctgg tgtacacctc gatcccctcg ccctgcttcg cgagctccca cccctggccg
     1321 ggcggagcgc caagcgccgg gagagagcag aaaagaacac cgagcatgac ggcaaagacg
     1381 taccgtatca taggtactct cctttcatcg cactgggaat cattcctcac cttgtatccg
     1441 aaaacacggg ctgataaaat acaaatattc cccatcctcg cattgtccag caaaaagccc
     1501 tttcataact gcagggctgt cgtaccggtg caatcacccc ggaatccgca acagggcgat
     1561 catgcgcgca gcgtgaggtc ttttcgaaga acgggtgaag ccgttgtcgc cgctgaccct
     1621 cctcttgatg tttcatgccg cttgtggcta tgattgccgg aatccacatt actgcgcttc
     1681 acgaaaa
//
LOCUS       DAOQTR010000507         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_96057_length_1683_cov_4.349509, whole genome shotgun sequence.
ACCESSION   DAOQTR010000507 DAOQTR010000000
VERSION     DAOQTR010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96057_length_1683_cov_4.349509"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1183
                     /locus_tag="PKN85_10420"
     CDS             140..1183
                     /locus_tag="PKN85_10420"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNT44871.1"
                     /translation="MNIRERLEEREGKVLSPWAQKSAATRGRMREEAECDIRPAFQHD
                     RDRITHSKSFRRLKYKTQVFLSPTGDHYRTRLTHTLEVSQIARTIGRALGLNEDLVEA
                     IALAHDLGHTPFGHSGEAVLNTLHPGGFRHYEQSLRVVDILEKDGEGLNLTMEVRDGI
                     VKHSKGKGDIIIRDDDERPLTKEAEVVRIADIIGYLNHDIDDAVRGNVISEEDIPRHL
                     RDNLGHSVSDRIDTMVRGVIWSTLQSGRMELGCGEELETHMLEMRDFLYVRVYESPVV
                     HADFIKCSKIIEDLYGYFLKNPDAFLKETDRNDFHDDPVACVRDFIAGMTDRYAFSLF
                     ERIFLPMPWSIPV"
     gene            1261..>1683
                     /locus_tag="PKN85_10425"
     CDS             1261..>1683
                     /locus_tag="PKN85_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT44872.1"
                     /translation="MMDKVYDPHSIEKKWYAEWLEKGYFTARNDSREEYFSMVIPPPN
                     VTGSLHMGHALNNTLQDIVTRYFRMCGVNSLWLPGTDHAGIATQNVVERELAKEGTDR
                     EALGREKFIERVWQWKKQSGGTIIEQLKRLGASCDWSRE"
BASE COUNT          397 a          482 c          495 g          309 t
ORIGIN      
        1 ccggccgaag gcgccggtgc cctttttgtc ttttactctt gaacttttga accctggaac
       61 tcttgaacct tctttccgaa gttcaggtcg ttgatattgt tacatcttta tgctacatta
      121 ggatgttggt tgcagccgaa tgaatatccg tgaaagactg gaagaacggg aggggaaggt
      181 cctgtcgccc tgggcgcaga agagcgccgc cactcgcgga aggatgcggg aagaggccga
      241 gtgcgacatc aggcccgcct tccagcacga ccgtgacagg ataacccaca gcaagtcttt
      301 ccgaaggctc aagtataaga cgcaggtctt cctttcgccc acgggagacc actaccggac
      361 acggctgacg cacaccctcg aggtttcgca gatcgcgcgg acgatcggcc gggcgctcgg
      421 cctcaacgaa gacctcgtgg aggccatagc ccttgcccac gacctcggcc atacgccctt
      481 cggccattcc ggcgaagccg tcctcaacac gctccatccc gggggcttca gacactacga
      541 gcaaagcctg agggtcgtgg acatactcga gaaggacggc gagggcctta acctgacgat
      601 ggaggtcagg gacgggatcg tcaaacactc caagggtaaa ggcgacataa tcatccgtga
      661 cgacgacgaa agacccttga cgaaagaggc ggaggtggtc cgcatcgccg acatcatcgg
      721 ctacctgaac cacgatatcg acgatgccgt gcggggcaac gtcatcagcg aagaggacat
      781 cccccgacac ctcagggaca atctcgggca cagcgtttcg gaccgcatcg acacgatggt
      841 gcgcggcgtc atctggagca ctcttcagag cggcaggatg gaactgggat gcggagagga
      901 actggagact cacatgctgg agatgcgtga tttcctctat gtcagggtct atgagagccc
      961 cgtcgttcac gcggacttca tcaagtgttc gaagatcatc gaggatctct acgggtattt
     1021 cctcaagaac cccgacgcgt tcctcaagga aacggaccgc aacgacttcc atgacgatcc
     1081 cgtcgcctgc gtccgggact tcatcgccgg catgacggac cgttacgcct ttagtctctt
     1141 tgaaaggatc ttcctgccca tgccatggag cattccggtg taggcggaag accggtgaac
     1201 gtgaaagaac aacgaccatc aagcgcgttt tgataggtac acacggaaag ggagctgaca
     1261 atgatggata aggtttatga cccccacagc atcgaaaaga aatggtacgc cgagtggctg
     1321 gagaagggat acttcacggc caggaacgac tccagggaag agtatttttc catggtcatc
     1381 ccgccaccca acgttacggg gtctcttcac atgggccacg ccctcaacaa cacgctgcag
     1441 gacatagtca cccggtattt ccggatgtgc ggggtcaatt ccctctggct tcccgggacc
     1501 gaccacgcgg gtatagccac gcagaacgtc gtcgagcgcg agctcgcgaa ggaaggcacc
     1561 gaccgcgagg ccctgggcag ggagaagttc atagaacgcg tctggcaatg gaagaagcag
     1621 tccggcggga ccatcatcga acagctcaag aggctgggag cgtcctgcga ctggtcgcgc
     1681 gag
//
LOCUS       DAOQTR010000508         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_96163_length_1682_cov_3.636755, whole genome shotgun sequence.
ACCESSION   DAOQTR010000508 DAOQTR010000000
VERSION     DAOQTR010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96163_length_1682_cov_3.636755"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKN85_10430"
     CDS             <1..846
                     /locus_tag="PKN85_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44873.1"
                     /translation="LDHDGRIRMDCSSPYAMASLIGLKDAYDIAFGNDPDFDRHGIVT
                     GGVGLMNPNHFLSVAIHYLFSSRKGWPGGAAVGKTLVSSSMIDRVAHSLGRRLLEVPV
                     GFKWFVECLLSSQCAFGGEESAGASFVKKDGTVWTTDKDGFIMDLLAAEIMAVTERDP
                     SEHYRELEARFGSPVYERLDAPANPEQKAILKKLSPQMIHAQELAGERITALITEAPG
                     NGAPLGGIKVMTENGWFAARPSGTEDIYKIYTESFKGREHLGRIQAEAQEIIGSVFRT
                     AGAGR"
     gene            complement(982..>1682)
                     /locus_tag="PKN85_10435"
     CDS             complement(982..>1682)
                     /locus_tag="PKN85_10435"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNT44874.1"
                     /translation="ADLILSSPLRITDIAMEVGFESSAAFSTAFKKHFNVTPSEFRRT
                     KGDGFRTEPLKFLNAIDMPGNKKRKLKADNTHFEIRTLPELTLLSISKRGYYSGCFFK
                     AAQDGYTELYDYLDTHGLSREVVHRISIFPELPYSMNDPDAVIHCGFSITKEIRPERP
                     FELLTIQEGNYAVFPYSGPYEYMYQMWIIAYYKCFLIGNEQVRDVAPFELYLNSPRDT
                     PPGDLRTEIHIPIV"
BASE COUNT          353 a          466 c          529 g          334 t
ORIGIN      
        1 ctggaccacg acggcaggat ccggatggac tgctcgtcgc cctatgccat ggcgagcctc
       61 atcggcctca aagacgccta cgacatcgcg ttcggcaacg acccggactt cgaccgccac
      121 ggcatcgtca ccggaggcgt cggcctcatg aacccgaacc acttcctgag cgttgccatc
      181 cactacctct tctcatcacg caagggctgg cccggaggcg cggccgtggg caagaccctg
      241 gtgagcagct ccatgatcga ccgggtggcg cacagcctgg ggagaaggct cctggaggtg
      301 ccggtggggt tcaagtggtt tgtggaatgt ctcctttcgt cacagtgcgc cttcggcgga
      361 gaggagagcg cgggcgcatc ctttgtgaaa aaggatggta cggtctggac cacggacaag
      421 gacggcttca tcatggacct gctcgctgcc gagatcatgg ccgtgaccga aagagacccg
      481 tccgagcatt accgggagct ggaggcccgc ttcggctcgc ccgtgtacga gcggctcgac
      541 gctcccgcga acccggagca gaaggcgata ctgaagaagc tctccccgca gatgatccat
      601 gcgcaggagc tcgcagggga aaggatcaca gccctgatta ccgaggcccc gggcaacggc
      661 gcacccttgg gcggcatcaa ggtgatgacc gaaaacggct ggttcgcggc ccggccctcg
      721 ggcaccgagg acatctacaa gatctacacc gagagcttca agggccggga gcacctgggc
      781 aggatccagg ccgaggcaca ggagatcatc ggctcggtgt tcagaacggc gggggcggga
      841 aggtagggca ccgcacatta ccgctcgcct tttccgtagc cggacacagg gattcaggga
      901 cggggccaca tgaaacatga ggaatgtgaa tccgcccggc tgcagtttgg cgcagcgctg
      961 atggccgtgc cggtttttcg atcacacgat agggatgtgt atttcagtcc tgaggtcacc
     1021 agggggcgtg tctcgaggtg agttgagata cagctcgaat ggggccacat cgcgaacctg
     1081 ctcgtttccg atgagaaagc atttgtagta tgcgataatc cacatctggt acatgtactc
     1141 atacggcccc gaatagggga agacggcata gttcccttcc tggatagtaa gaagctcgaa
     1201 aggccgttcg ggccgaatct cttttgttat ggaaaacccg caatggatca ccgcatcagg
     1261 gtcgttcatg gaatagggca gctcgggaaa gatgctgatg cggtgtacaa cctcccggct
     1321 gaggccatgg gtgtcgagat agtcatataa ttcagtatag ccgtcctgcg ccgccttgaa
     1381 aaagcagcct gaataatatc ccctttttga tatggaaagg agcgtcagtt cgggaagggt
     1441 ccttatttcg aaatgcgtgt tgtctgcttt cagtttcctc ttcttattgc cgggcatgtc
     1501 gattgcgttc aggaacttca ggggttcggt gcggaacccg tcccctttgg tccgcctgaa
     1561 ttccgacggg gtgacattga agtgcttttt gaacgcggtg gaaaaggcgg ccgaactctc
     1621 gaaccctacc tccatggcta tgtcggtgat cctcagcggg gaactcagga tgaggtcggc
     1681 cg
//
LOCUS       DAOQTR010000509         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_96622_length_1677_cov_4.027127, whole genome shotgun sequence.
ACCESSION   DAOQTR010000509 DAOQTR010000000
VERSION     DAOQTR010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96622_length_1677_cov_4.027127"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKN85_10440"
     CDS             complement(<1..369)
                     /locus_tag="PKN85_10440"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44875.1"
                     /translation="MVISIANQKGGVGKTTTAVNLSASIAVSERKTLVIDMDSQCNTT
                     SGFGIPYNNVVGHIYSVLIGEKTMAEVIRPTAVPYLDIIPAHPDLIGAEIELLDTTER
                     EYALKKAIGELGDTYSYIFVD"
     gene            complement(403..1020)
                     /locus_tag="PKN85_10445"
     CDS             complement(403..1020)
                     /locus_tag="PKN85_10445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT44876.1"
                     /translation="MDLKGLIAETLEHFLISPGEAGLVSLVRYIEELERWGSRMNLVG
                     LKDMERVCRELLADSFFLHAFVGDRRRLVDVGSGSGILAVPLAILNGSLEVLAVDKSL
                     KKVQFQRHVRRTLSLTNLRPVGGRIEAIDPLDADGLVAKAYGTTGAVIAASNRHLAAG
                     GLTFVLKGKGQRDENFPGHRLEKSLGYSLPGVARDYRLLIYKKIC"
     gene            1067..>1677
                     /gene="bamD"
                     /locus_tag="PKN85_10450"
     CDS             1067..>1677
                     /gene="bamD"
                     /locus_tag="PKN85_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNT44877.1"
                     /translation="MRKFFIIFIALFVLVSCSSKTPKKAENPVDLYVAGVNLMNTKKY
                     DKAVEKFKAIREQYPFDPMALVAAVKLGDTYFLKKDYVLAAGTYEDYFKAHPDEENIP
                     YVLFRLGECYEKLSPSIDRDQANTVKGIERITYLRNRYPTSSYAKESESRLKRFIQKL
                     ADRELYVGEFYVRTAQYNACVIRLEDMLKRYPESKNMDKALFYL"
BASE COUNT          375 a          495 c          466 g          341 t
ORIGIN      
        1 gtcgacgaat atgtacgaat aggtgtcccc cagttccccg atggccttct tcagggcgta
       61 ctcccgctcg gtcgtgtcga gaagctcgat ctcggcgcct atgaggtcgg gatgggcggg
      121 gatgatatcc agataaggga ccgcggtggg acggatcacc tcggccatcg tcttctcgcc
      181 gatgaggacg ctgtaaatgt ggccgaccac attgttgtaa ggaatgccga acccggacgt
      241 ggtgttgcac tgggaatcca tgtcgatgac gagggtcttc cgttcggata ccgcgatgga
      301 cgccgagagg ttgacagcgg ttgtcgtctt gccgaccccg cccttctggt tcgcgatgga
      361 gatcaccatg acagtttcat taccatacca cattaccggg cactagcaaa tttttttgta
      421 tatgagaagg cggtagtccc tggcaacccc cggcaatgaa tagccgaggc tcttctccaa
      481 cctgtggccc gggaagttct cgtcccgttg tccctttccc ttgaggacaa aagtcagccc
      541 tcccgccgcg agatgcctgt tcgacgcggc gatgacagcc cccgtggtcc cgtaggcctt
      601 cgcgacgaga ccatcggcat cgagggggtc gatcgcctct atccgtcccc cgacgggccg
      661 gaggttggtg agcgagaggg tgcggcggac gtgacgctgg aactggacct tcttgaggct
      721 cttgtcgacg gccaggacct caagggatcc gttgaggatg gccagcggaa cggcaaggat
      781 gccggacccc gaaccgacgt cgacgagacg ccgcctgtcc cccacaaaag catggagaaa
      841 gaaggagtcc gccaggagct cgcggcagac acgctccatg tccttgagcc ccacgaggtt
      901 catcctgctt ccccagcgtt cgagctcctc aatgtaacgc acgagggaca ccaacccggc
      961 ctctccggga gagatgagga aatgctccag agtctcggct atcagtcctt tcaggtccac
     1021 gcgttaaaga atagcataat tctttttctt ctgttatact aagcccatgc gaaaattctt
     1081 cattattttc attgcacttt tcgtgctcgt ttcgtgctcc tcgaagaccc ctaagaaggc
     1141 cgagaacccc gtcgacctct acgttgccgg cgtgaacctg atgaacacca agaaatatga
     1201 caaggccgtg gaaaagttca aggccatccg cgaacagtat cccttcgacc ccatggccct
     1261 cgtcgccgcc gtcaagctcg gcgataccta cttcctgaag aaggactatg tcctggccgc
     1321 cggcacctac gaggactatt tcaaggccca cccggatgaa gagaacatcc cctacgtcct
     1381 cttccgtctc ggcgaatgct acgagaagct ttcgcccagc atcgaccgcg atcaggcgaa
     1441 cacggtgaaa ggcatcgaaa ggataaccta cctgaggaac cggtacccca cgagctccta
     1501 cgcaaaggag tcggaatcga gattgaaaag gttcatacag aagctggctg accgggagct
     1561 ctacgtgggc gaattctatg tcaggaccgc ccagtacaac gcatgcgtga tcaggctcga
     1621 ggacatgctg aaaagatatc cggagtcaaa gaacatggac aaggcccttt tctacct
//
LOCUS       DAOQTR010000510         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_96715_length_1676_cov_4.027761, whole genome shotgun sequence.
ACCESSION   DAOQTR010000510 DAOQTR010000000
VERSION     DAOQTR010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96715_length_1676_cov_4.027761"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKN85_10455"
     CDS             complement(<1..339)
                     /locus_tag="PKN85_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44878.1"
                     /translation="MMEFFLSKFWAFLVSMVIMAVLVQGMQLDARMDRNEALNDLAGE
                     LERMFDDLAEGGPGLEVTIELARVLPPTAILTLNSGYASLQDQGGEVLFTVPAMTMNL
                     ENEHGDVAEVE"
     gene            complement(336..779)
                     /locus_tag="PKN85_10460"
     CDS             complement(336..779)
                     /locus_tag="PKN85_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44879.1"
                     /translation="MRKDRRGIEGLPLRLMPVAILISLALPTMLSCMQNATSSIAEGR
                     AAEVAEDIAAIAEEMSAGGPGNVRTMTVPSDIPDGITFGIGGRNGTVDCTRITWTIDG
                     RENARYLSGVRIITESGGPLTISSGNSIRLVCPSGTWGTVKAVLA"
     gene            complement(784..1263)
                     /locus_tag="PKN85_10465"
     CDS             complement(784..1263)
                     /locus_tag="PKN85_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT44880.1"
                     /translation="MRFSKKRIGGDREGGIEGLPLQLMIMVIVAGLGTAILVGWMGGL
                     SAPEAIASVNGNPNEILLTDGDGDGTYENDDAALTIIVRDQNGDPVEGASVVLDGCEV
                     RNSDGSLVYGTTGAEGKVTFDGLSLSHYGDGIGFITVTVTKGGMGTDSSLQVPVICR"
BASE COUNT          357 a          535 c          440 g          344 t
ORIGIN      
        1 ctcgacctcc gctacatctc cgtgctcatt ttccaggttc atcgtcatag ctggaactgt
       61 gaaaaggacc tcacctccct ggtcctgcag cgaggcgtat ccggaattta gggtcaatat
      121 ggccgtcggg ggcagtaccc gagcgagttc gatagtaacc tccaacccag gaccgccttc
      181 cgccaggtcg tcgaacatcc tttccagctc gccggccagg tcgttcaacg cttcgttccg
      241 gtccattcgc gcgtcgagct gcatcccctg gaccagcacg gccatgatga ccatcgacac
      301 caggaaggcc cagaacttgg agaggaagaa ctccatcatg ccaataccgc ctttacggtc
      361 ccccaggtcc cggacgggca tacgagccgt atcgaattgc cggacgagat ggtcagaggt
      421 cccccgctct cggttatgat cctcacgcca gacaagtacc tggcgttctc ccgcccgtcg
      481 atggtccagg ttatcctggt gcaatcgacg gtaccattcc ggcctccgat accgaaggtg
      541 atgccgtcgg gaatgtcgga cgggaccgtc atggtccgca cgttcccggg accgccggcg
      601 gacatctcct ctgcgatggc tgcgatgtcc tcagcgacct cggcagccct gccctcggcg
      661 atgcttgaag tggcgttctg catgcaggaa agcatggtag gaagggccag ggagatcaag
      721 atagccaccg gcatcagccg gagcggcagc ccctcgatcc cccttctatc ctttctcacc
      781 gtctcacctg cagatcactg gtacctggag cgaggagtcc gttcccatgc cgcccttggt
      841 cacggtgacg gttatgaagc cgatgccgtc gccgtagtgg gataagctca gcccgtcgaa
      901 cgtcaccttt ccctccgcac ctgtggtccc atagaccagg gagccgtcgg agttgcgcac
      961 ctcgcatccg tcaagcacca ccgaagcgcc ttccaccggg tcgccgttct ggtcgcgtac
     1021 gattatcgtg agcgcggcgt cgtcgttctc ataagtaccg tccccgtccc catccgtgag
     1081 caatatctcg ttcgggttgc cgttgacgga ggctatcgcc tccggcgccg agaggccgcc
     1141 catccatccc accaggatgg ccgtcccgag accggccact atgaccatta tcatcagctg
     1201 cagcggcaat ccctcgatcc cgccttccct gtccccgcct attcgtttct tagagaacct
     1261 cattgtatct ccgaaatctg gaaagccaag ggatgcctag accccctcgc ttacagtgag
     1321 actaactagc ggaccgcatt cgtcaggtca aaaacgaatt ttaccaataa tattcttgat
     1381 tcttatcaga aaacgaatat tccgtccgaa atcaaaagaa tttatgagca tagagaatcg
     1441 aaattattat aaaaacatgt tacattccct gccacgcagt cgcatgcgga gccatggcgc
     1501 ctgaggtctc aagatgttca agaaggtgct gatagcgaac agaggagaga tcgccattcg
     1561 ggtgatcaac acctgcaaga ggatgggcgt gccgaccgta ggcatctaca ccccctcgga
     1621 caagcactgc aagcacgttt cgctggcaga caagagcgtg gagctgcaca ccgacg
//
LOCUS       DAOQTR010000511         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_97666_length_1666_cov_3.987585, whole genome shotgun sequence.
ACCESSION   DAOQTR010000511 DAOQTR010000000
VERSION     DAOQTR010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97666_length_1666_cov_3.987585"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKN85_10470"
     CDS             complement(<1..384)
                     /locus_tag="PKN85_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNT44881.1"
                     /translation="MIERYTRERMARVWTDENKYRKWLDIEVLICEAYTKLGLIPEED
                     MKNIREKANFEVARVLEIEERTRHDVVAFIENVAEYVGPSSKYIHMGVTSSDILDTSF
                     SCLLNEAADILIEDVVALMEVLKEKA"
     gene            complement(875..1342)
                     /locus_tag="PKN85_10475"
     CDS             complement(875..1342)
                     /locus_tag="PKN85_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44882.1"
                     /translation="MWNWKPFFDDESISAFCDLDQIVDTEADDDGLYASPDCYRPLPL
                     RFGVFLSIVLKKKDDMARYLEERKARSLPLKGYKSYRYSLCLAEIDARTMRCRVLPAG
                     DYDSKDRELGDSCIVTDITPPILPGITDEWKPIRSKRSHPMVRALAKMFFPKE"
     gene            complement(1349..>1666)
                     /locus_tag="PKN85_10480"
     CDS             complement(1349..>1666)
                     /locus_tag="PKN85_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44883.1"
                     /translation="ATPKGTRKFRNLTGTKDVALLIDNRSDQKKGFVQTQAITVIGTA
                     RPVRRGRAWLEYAGIFLKKHPELEEFLNASTTALVVVDVTSCVHVGHFQTVTVADWAN
                     KES"
BASE COUNT          322 a          501 c          473 g          370 t
ORIGIN      
        1 ggccttctcc ttgaggacct ccatgagcgc gacgacgtcc tcgatgagga tgtccgccgc
       61 ttcgttgaga aggcaggaga aggacgtgtc cagtatgtcc gacgaggtga cgcccatgtg
      121 gatatacttg gaggagggtc cgacgtactc agcgacgttc tcgatgaagg ccaccacatc
      181 gtggcgcgtc cgctcttcga tctcgagcac cctggcgact tcgaagttcg ccttctccct
      241 gatgttcttc atgtcttctt cagggatgag gccgagcttc gtatacgcct cgcagatgag
      301 gacctctatg tcgagccact tccggtactt gttctcatcc gtccagaccc gagccatccg
      361 ttcccgcgta taccgttcga tcatgcccac tatataaccc gacggaagga atgatgtcaa
      421 tttgaaaaca aggacattat ctctcgcccg ttcgtcgcgt tcgctcctct ctcaagaccg
      481 cagaggacgc aaagagagac ggaaaagaga gaaaagactg ccgccggatc tcggagcctc
      541 ccaaaatccg gcggccttct cgtcccgccg tcccggcggg agagaaaaga atatccttat
      601 tctgaagaga ctattctcct ctcatccccc ctgtagtcat atgtcctata ggtcctgtac
      661 cccatactcc aacccccata ctacctcacc ccacaactcc tctcctcccc ccatcagccc
      721 cacctgaaaa gaaaactctt cttttgcctc ccgtcgcaga cgggatgcaa agacggccag
      781 atccaggcgg gcgccgggat ctggccgagt cttctctctg cgtgctctct tcatgaactc
      841 tgcgccctcg agcgaagcgg gcgtgagatg gtctctattc tttcgggaag aacatcttcg
      901 ccagggcgcg gaccatggga tgggagcgtt ttgagcgtat gggtttccat tcgtcggtta
      961 tgccggggag gatgggcggg gtgatgtcgg tgacgatgca gctgtcgccg agctcgcggt
     1021 ccttcgaatc gtaatcaccc gcgggcagga cgcggcagcg catggtgcgg gcgtctattt
     1081 ccgcgaggca gagggaatag cggtatgact tgtacccttt gaggggcagg gaacgggcct
     1141 ttcgctcctc gagatagcgc gccatgtcgt ccttcttctt gaggacgatg gacaggaaca
     1201 cgccgaagcg aaggggcagg ggacggtagc agtccggcga cgcgtacagg ccgtcgtcgt
     1261 cggcttcggt gtcgacgatc tggtccaggt cgcaaaaagc gctgatgctt tcgtcgtcaa
     1321 agaatggttt ccagttccac atgatccgtt aactctcctt gttcgcccag tctgccacgg
     1381 tgacggtctg aaagtgaccc acgtggacgc agctcgtcac gtcgacaacg acgagcgccg
     1441 tcgtcgaggc gttgagaaac tcctccagtt caggatgctt tttcaggaag atccccgcgt
     1501 attccagcca tgccctgcca cggcggacag gccttgccgt gccgatgacc gtgatggcct
     1561 gcgtctggac gaagcctttc ttctgatcgg agcggttgtc gatcaagagt gccacgtctt
     1621 tcgtgcccgt gaggttcctg aacttccggg tgcccttcgg cgtcgc
//
LOCUS       DAOQTR010000512         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_97897_length_1664_cov_3.120572, whole genome shotgun sequence.
ACCESSION   DAOQTR010000512 DAOQTR010000000
VERSION     DAOQTR010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97897_length_1664_cov_3.120572"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="PKN85_10485"
     CDS             complement(<1..1645)
                     /locus_tag="PKN85_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44884.1"
                     /translation="MEFNRTLLQTSPAFFVALSAEGKTLLMNQSMLDALGYTMEEVSG
                     KDYAADFVLPEERPLVLGIFSRIVSSNEPVLCENRVVASDGRPLLVEWHGTAVRDDQG
                     ALQYFFGVGIDLTERRKAEDALKAETEKLQMLSENAPVGMVLINHEGRFTYVNTKFRE
                     MFGYDLGDVPDGRSWFRKAYPDKQYRTMVISTWVEDFVGAEPGERKPRVFTVNCADGK
                     QRLITFVTLRLTSGEHLMVCEDMTELKRLEDRLRQSQKMEAIGNLAGGVAHDFNNILT
                     TIMGYGSLVQFEVGDNEKAKSHVEQILSAAQKAANLTQSLLSFSRHRTVTLKPLNLND
                     SLRGAEKLLKRLLTEDIDFRLALTDRDTTILADATEIDQILFNLVSNARDAMARGGRL
                     TIETDIVEMDSAYLEAHGFGQLGKYVTIAVSDTGTGMDEATQERMFDPFFTTKEIGKG
                     TGLGLATVYGIVSQHNGYINVYSEPGKGATFRIFFPLAEPAAKEEQSDIMDVKGGSET
                     ILVAEDNEAVMTLIKEVLTRHGYHTLEAADGEKAVEIFRA"
BASE COUNT          300 a          508 c          470 g          386 t
ORIGIN      
        1 tggccctgaa gatctcgacc gctttctcgc cgtcagccgc ctccagcgtg tggtagccat
       61 gcctcgtgag gacctccttg atgagggtca tgacggcctc gttgtcctcg gcaacgagga
      121 tggtctcgct gccccccttc acgtccatga tgtcggattg ctcttccttt gccgcgggct
      181 ccgcgagggg gaaaaagatg cggaaggtcg cgcccttgcc gggttcgctg taaacgttga
      241 tatagccatt gtgctgggaa acgatgccat agacggttgc gaggccgagg cccgtcccct
      301 tgccgatctc cttcgtggtg aagaatgggt cgaacatccg ttcctgcgtg gcctcatcca
      361 tgcctgtgcc ggtgtccgaa acggcgatgg tcacatattt ccccagctgc ccgaacccgt
      421 gggcttcaag gtaggcgctg tccatctcga caatgtccgt ttcgatggtg agcctgccac
      481 ccctcgccat cgcgtcgcgc gcgttgctga caaggttgaa aaggatctga tcgatctccg
      541 tcgcatcggc aaggatcgtc gtgtcccgat ccgtgagcgc cagcctgaag tctatgtcct
      601 ccgtgagaag cctcttcaaa agcttctccg ctcccctcag ggaatcgttg agattgagcg
      661 gcttcaaggt gaccgtcctg tggcggctga aggaaaggag gctctgcgtc aggttggccg
      721 ctttctgggc cgcggacagt atctgctcca catgggactt cgccttctcg ttgtcgccga
      781 cctcgaactg gacaagactc ccatatccca tgatggtggt caggatattg ttgaaatcgt
      841 gggccactcc tcccgccagg tttccaatgg cctccatctt ttgagactgg cggagccggt
      901 cctcgaggcg tttgagctcg gtcatatcct cgcagaccat gagatgttcc cccgaggtga
      961 gccgcagcgt tacgaaggtg atcagcctct gcttgccatc ggcgcagttg acggtgaaca
     1021 cgcggggttt tctttcgccg ggctcggcgc cgacaaaatc ctccacccag gtggatatca
     1081 ccatcgtcct gtactgcttg tcgggatagg cctttctgaa ccaggacctc ccgtcgggca
     1141 cgtctccaag gtcgtatccg aacatctccc tgaactttgt gttaacgtag gtaaatcggc
     1201 cttcgtgatt gatgagcacc atcccgacgg gtgcgttctc ggacagcatc tgcagttttt
     1261 ccgtttccgc cttgagggcg tcttccgcct ttctgcgctc cgtcaggtca atgccgacgc
     1321 caaagaagta ctgcagcgcg ccctggtcat ccctgactgc cgtgccgtgc cattccacga
     1381 gaagcggtct gccatcgctc gctacaacgc ggttctcgca cagcacgggc tcgttggagg
     1441 aaactatgcg ggagaagatc cccaggacaa gcggtctttc ttcgggaaga acaaagtcgg
     1501 cagcatagtc cttcccggaa acctcttcca tggtataccc caacgcatcc agcatcgatt
     1561 ggttcatgag aagcgtcttt ccctccgcgc tcaaggcgac aaagaaggcc ggggatgtct
     1621 ggagcagggt cctgttgaac tccatctcgt cccggatcat gatc
//
LOCUS       DAOQTR010000513         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_98387_length_1659_cov_3.071696, whole genome shotgun sequence.
ACCESSION   DAOQTR010000513 DAOQTR010000000
VERSION     DAOQTR010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98387_length_1659_cov_3.071696"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKN85_10490"
     CDS             <1..606
                     /locus_tag="PKN85_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HNT44885.1"
                     /translation="LFFWVYPRVVSMSMPNYWLGLDSLEKSGHLHHNRSMDERHWYVV
                     NTKARNEERAAVNLAQGGIDVLAPKLRFKRWREGKFTDIIEPMFPGYIFVKFHPVDEF
                     RLVKYTRGIKTIVNFNGRIIPLQEGLIDFIRERLTDGVATVEKPTFQKGEKLIIQEGP
                     FKGFGGIFEQELDGRERIAILLEGVDYCARMEIDRELVAKA"
     gene            743..>1659
                     /locus_tag="PKN85_10495"
     CDS             743..>1659
                     /locus_tag="PKN85_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44886.1"
                     /translation="MDISKETSRQIGLIVNRRGLVEYVIVGDNRRIEIPPLSTERTGR
                     ARFRGVRFIHTHLRGELLSREDLTDLALLQLDLVACITEMRGERGEAVHVGYLIPENR
                     EGRTWDFMEPGPVSELDLDFTEFITELENEFVRERGRFYVTKDKHDRCVIVSVVIPGR
                     EKNVDDYTAEMKDLCYSAGITALDTVVQRPKELHPRYLIGRGKVEEIVMKCQQLGADL
                     LVFDEELTPGQIKNISEITELKVIDRNQLILDIFAGRANTTESKIQVELAQLRYILPR
                     LTEKSTAFSRLMGGIGGRGPGEQKLEVDRR"
BASE COUNT          391 a          483 c          499 g          286 t
ORIGIN      
        1 cttttcttct gggtataccc cagggtggta agtatgtcca tgcccaacta ctggctcggc
       61 ctcgattcac ttgaaaaatc ggggcattta caccataata gaagtatgga cgaaagacac
      121 tggtatgttg tgaacaccaa ggcgaggaac gaggagcgcg ccgcggtgaa cctcgcccag
      181 ggagggatcg atgtcctcgc cccgaagctg aggttcaagc gctggcgcga gggaaagttc
      241 acggatatca tagagcccat gtttcccggg tacatcttcg tcaagttcca ccccgttgac
      301 gagtttcgcc tcgtcaagta cacccggggc ataaagacga tcgtcaactt caacggcagg
      361 ataatccccc tccaggaagg gctcatcgat ttcatcaggg agcgcctcac cgacggcgtg
      421 gccacggtgg agaagccgac ctttcagaaa ggggagaagc tcatcatcca ggaaggccct
      481 ttcaagggtt tcggcggcat cttcgaacag gagctcgacg gcagggaacg gatcgccatc
      541 ctcctggaag gcgtcgatta ctgcgcgcgc atggagatcg accgggaact cgtggcgaag
      601 gcgtgacaga cagaggtatg ccatagagct ttacggaaac acccagggtc tcaaccacct
      661 cataaaaaga cgtctcacca acctctacaa gaaacgcatc gagccttccg ccgtcatcac
      721 ggctgacctg gcgcggcaac tcatggacat ttcgaaggag acgtcgcgcc agatagggct
      781 tatcgtcaac cgccgcgggc tcgtagagta cgtcatcgtc ggagacaacc gccgcatcga
      841 gatccctccc ctttcgacgg agcggacggg ccgggcgcgc tttcgcggtg tgaggttcat
      901 ccacacccac ttaaggggtg agctcctctc ccgcgaggac ctgactgacc tcgccctcct
      961 gcagctcgat ctcgtggcct gcatcaccga gatgcggggg gaaaggggcg aggcggtcca
     1021 tgtggggtac ctcataccgg agaacaggga gggcaggacc tgggacttca tggagccggg
     1081 cccggtgtcg gagttggacc tcgacttcac cgaattcatc accgaactgg agaacgagtt
     1141 tgtcagggag cggggcaggt tctacgtgac gaaggacaag cacgaccgct gtgtcatcgt
     1201 tagcgtcgtc atccccggaa gagagaagaa cgtcgacgac tataccgcgg agatgaagga
     1261 cctctgctat tcggcgggga tcaccgccct cgacaccgtg gtgcagagac ccaaggaact
     1321 ccacccccgg tacctcatcg gaaggggaaa ggtcgaggag atagtcatga aatgccagca
     1381 gctcggcgcg gaccttctcg tcttcgacga agaactgacg ccgggccaga taaagaacat
     1441 atcggagata acggaactga aggtcatcga ccgcaaccag ctcatcctgg acatatttgc
     1501 cggccgggcc aacaccacgg agtcgaagat acaggtggag ctcgcccagc tgcgctacat
     1561 actcccccga ctcaccgaga agagcacggc attctcgcgc ctcatgggag gcatcggagg
     1621 ccgcggcccc ggcgaacaga agctggaagt ggaccggcg
//
LOCUS       DAOQTR010000514         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_100563_length_1638_cov_3.596336, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000514 DAOQTR010000000
VERSION     DAOQTR010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100563_length_1638_cov_3.596336"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PKN85_10500"
     CDS             <1..1131
                     /locus_tag="PKN85_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44887.1"
                     /translation="GGPEDPLRFARLADDGQLSDKGFYEIIDIYENLKSSPEYQEGPL
                     GSIIKIRIAQTYAHRRDWENALASYLRAWTDTKKDDPVHIYVQTEAEKCVLARLQELF
                     RQEAYDMVLDLYTEYRDSFMHDIQDLETRFILATSFHRLGHADQARGMFMSCIEKPSS
                     RSEEALYSLYAIDHARGDYAGALKWNTRYLESYPRGNNASVMRENRGPLLYYTSNLEE
                     AISYLEQITDKGDEKALTALSMLADAHEKLGDPDRTIATLEKIIGFPEAVRSPVIEKA
                     LYVRAIRLKETSETARSQDLFARLLDQYPQSAYRDWALYHLAEISRARGDTGEAARLL
                     DTVIQRSTDKVLHELAMSLQGELGLAKDLREFSNLKDRFREK"
     gene            1138..>1638
                     /locus_tag="PKN85_10505"
     CDS             1138..>1638
                     /locus_tag="PKN85_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT44888.1"
                     /translation="MGIAILDNDKSTVSILAEIIDEMGESPCPFTDKDKFLGAIDPKS
                     HCVAVIADDFLDVIEGTKQVYPSIEILAMGSLDGSRCIQAGAAHHLGKPFSADSFRQV
                     LQTLLIGMKARPVSENGSFIAADRSMQRIIEVLDKVAPTQAPVLIQGESGTGKEVFAR
                     YLHQHSR"
BASE COUNT          389 a          491 c          463 g          295 t
ORIGIN      
        1 ggcggtccag aagatccact tcgattcgcc cggcttgccg acgacgggca gctctcagat
       61 aaggggttct acgagatcat cgacatctat gagaatctca aatcgagccc tgagtatcag
      121 gaagggcctc tgggatccat catcaagata cgcatcgcac agacgtatgc tcaccgcaga
      181 gactgggaga acgccctggc ctcttacctg cgcgcgtgga cagacacgaa aaaggacgac
      241 cccgttcata tctatgtgca gacagaggct gaaaagtgcg tcctcgcccg cctgcaggaa
      301 ctcttccggc aggaggccta cgacatggtg ctggacctct acacagagta ccgggacagc
      361 ttcatgcatg acattcagga cctggaaact cgattcatcc tcgccacgtc tttccatcgc
      421 ctcggacatg ccgaccaggc tcgcggcatg ttcatgtcct gcatcgaaaa accgtcatcc
      481 agaagtgagg aagccttgta ctccctgtat gccatcgatc atgcgcgggg agactatgcc
      541 ggcgccctga aatggaacac ccgatatctc gaatcatacc cccgggggaa taacgcctct
      601 gtcatgaggg aaaaccgggg accgctgctc tactacacaa gcaaccttga ggaggcgata
      661 tcatacctcg agcagataac ggacaagggg gatgaaaagg cgctgaccgc cctgtccatg
      721 ctggcggatg ctcatgaaaa gctgggcgac cccgaccgga cgattgcaac cctagaaaag
      781 ataatcgggt ttccggaagc cgtgaggtct ccggtcatcg agaaggccct gtatgttcgg
      841 gccatcaggc tcaaagagac gtccgaaacc gcacggtcgc aggatctctt cgcgaggctg
      901 ctcgatcagt acccccagtc ggcgtaccgg gactgggcgc tctaccacct tgcggagatt
      961 tctcgcgcac gaggagacac cggggaggcg gcacggcttc tcgataccgt catccagcgc
     1021 agcacggaca aagtcctcca cgagctcgcc atgagccttc agggcgaact ggggctcgcg
     1081 aaagacctgc gcgagttttc gaatctcaaa gacagattca gagagaagta gcgatcaatg
     1141 ggcattgcaa tcctcgacaa cgacaaatcc acggtctcca ttctggcgga gatcatcgat
     1201 gaaatggggg aatcgccctg cccgttcacg gacaaggaca aatttctggg ggccatcgac
     1261 cccaagtccc actgcgtggc tgttatagcc gatgatttcc tggatgtcat cgagggtacg
     1321 aagcaggtat atccgagcat agaaatcctc gccatgggct cgctcgacgg cagccggtgc
     1381 attcaggccg gagcagccca ccatctcggc aagccgttct ctgcagacag tttccggcag
     1441 gttctccaaa cgctgctgat cggcatgaag gcccggccgg tctcagaaaa cggatcgttc
     1501 atcgccgccg acaggagcat gcagaggatc atcgaggttc tcgacaaggt cgcgcccacc
     1561 caggcgccgg tactgatcca gggcgagtcc ggcaccggca aggaggtctt cgcgcgctac
     1621 ctccaccagc acagcagg
//
LOCUS       DAOQTR010000515         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_100584_length_1638_cov_2.929248, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000515 DAOQTR010000000
VERSION     DAOQTR010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100584_length_1638_cov_2.929248"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKN85_10510"
     CDS             complement(<1..639)
                     /locus_tag="PKN85_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44889.1"
                     /translation="MRQSQATVVLVQGLKAGEEERRFRSQEAGMTGVPGSRPQYSGAK
                     LPGFVLWFLFLVVCILGGLPVFADQVDIWMQTPDNWEDRRSELGRDLMKQLFAPGGVA
                     VIEVYGARGSNPGLRVIADKMEEQMLARGAAYLQTRLTDNLRKNADGHDAIVREYSGV
                     HNGIPLRAIAVYTYGNGGAVAAFGFYARNEGAAYHNAVWASIGSIRFSPPGAS"
     gene            complement(979..>1638)
                     /locus_tag="PKN85_10515"
     CDS             complement(979..>1638)
                     /locus_tag="PKN85_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT44890.1"
                     /translation="GVFLGACHLGECNYITHGNYQAQNMVLLFKRIMEHIGLNPERLR
                     INFMSGAEANVFTESVNDFVKTIKGIGPLGKAEGMDAAELKTRLAEVKKLIPYFKIVN
                     REKLTTRLEGHDHAEAEKLFTKEEIDRQFAEVFSYWIDPEKCQACMTCARRCPVDAII
                     SEKQKIHIVDQDKCVRCGTCFEVCPPKFRAISKITGPVPPPPPEDKRTVVRKGKEEAA
                     G"
BASE COUNT          324 a          514 c          445 g          355 t
ORIGIN      
        1 tgacgctccc ggcggagaaa agcggatgct gccgatactc gcccaaacag cattgtggta
       61 cgcagccccc tcattccggg catagaaccc gaaggcggcc actgctccgc cgttgccata
      121 ggtgtaaacg gctatggcac gaagcgggat gccgttgtga accccggagt actccctgac
      181 gatggcatcg tgaccgtccg cgttcttccg cagattgtcg gtcaaccttg tctgcagata
      241 cgcagccccc cgggccagca tctgctcctc catcttgtcg gcgatcaccc tgaggccggg
      301 gttgctgccc cgtgccccgt agacctcgat aacggcgacg cccccggggg cgaagagctg
      361 cttcatcaga tcgcgaccga gttcgctcct tctatcctcc cagttgtcag gtgtctgcat
      421 ccagatatcg acctggtccg cgaagaccgg cagccctccc aaaatgcaga cgacgaggaa
      481 gagaaaccac agcacgaaac caggcaattt cgcccccgaa tactggggac gagacccggg
      541 aacaccggtc atgccggctt cttgcgaccg gaaccgcctt tcctcctccc ctgccttcag
      601 cccctgaacc aacaccaccg ttgcttgcga ttgcctcatg tgatacctcc tttgttcagc
      661 atccggggat ttcgctcccg tcccggcggt ccgccgaggg cgtccatgat ttctcccgtc
      721 aagggaaaaa gatggcttat ctcaagaagg gactcccagt cctcctttgc atgcgttcgc
      781 ttcttcagat ctgcagggct tgcggaccgg ccgtgagagg atgccatgac acatccgggc
      841 ctcatggaga acaagcgccc tatcacgtgc atgcacgatc cggaacgcgc atgcgggcgc
      901 taaaaaaagg aagagtatcc atccggcttc cttcaccgga tggatactcc gacattctac
      961 agcatatcta cagcggtatc aacccgctgc ttcttccttg ccttttctga cgacggtcct
     1021 cttgtcctcg ggcggaggag ggggcaccgg gccggtgatc ttcgatatgg cgcggaactt
     1081 cggaggacag acctcgaagc acgttccgca acgaacacac ttgtcctgat cgacgatgtg
     1141 gatcttctgc ttctcgctga tgatggcgtc gacagggcag cgcctggcac aggtcataca
     1201 ggcctggcac ttctcggggt cgatccagta tgagaagacc tcggcgaact gcctgtctat
     1261 ctcttccttc gtgaagagct tctctgcctc ggcatggtcg tgaccctcga gacgggtcgt
     1321 gagcttttcc ctgttcacga tcttgaagta agggatgagc tttttcactt cagcaaggcg
     1381 ggtcttgagc tctgcggcgt ccatgccctc cgcttttcca aggggaccga tacccttgat
     1441 ggtcttcacg aaatcgttaa cggattccgt gaaaacgttc gcctcggcac cggacatgaa
     1501 attgatccgg agcctctcgg gattgaggcc tatatgctcc atgatcctct tgaagagcag
     1561 aaccatgttc tgggcctgat aattcccgtg tgtgatgtag ttgcactcgc ccaggtggca
     1621 ggcgcccagg aacacacc
//
LOCUS       DAOQTR010000516         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_101005_length_1634_cov_3.327422, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000516 DAOQTR010000000
VERSION     DAOQTR010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101005_length_1634_cov_3.327422"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..707)
                     /locus_tag="PKN85_10520"
     CDS             complement(243..707)
                     /locus_tag="PKN85_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44891.1"
                     /translation="MLRARPGSWFSWKFNILDENDQIIAGIDIRWMREAGELHLGGRS
                     YRLFREGLFGGAFILEEDGMELARAEKPSSLFRSFTVTAKGRAYRLHAASPVGRTFIL
                     SENDRTIGVIRPENAFTRKAVIDLPEEIALPVKIFMAWLVFVLWKRDEESSS"
     gene            952..>1634
                     /locus_tag="PKN85_10525"
     CDS             952..>1634
                     /locus_tag="PKN85_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44892.1"
                     /translation="MEREQRIAVASEIMELFAELTGLVPGGVSPRRYLWTDAYGVCNF
                     LGLYRETGDVAYRDLALRLVDDVHHTLGRHRKGDARQGWISGLDEDGGEDHPTARGLR
                     IGKKLGERGPDEPLDENGEWDRDGQYFHYLTKWMHALNLVSRSTGDFTYNRWAMELAK
                     AAHRGFVYTVPGTRTRRMFWKMSTDLSYPLVSSMGHHDPLDGLITFIQLEAMEDPDRP
                     EHLGLAAEI"
BASE COUNT          337 a          462 c          508 g          327 t
ORIGIN      
        1 cgccgacccg ccgataacga ctattcttct tgctctggtc atgaagtctc tcctttttgg
       61 tggctatgac gcacagcgga tcccgatgtt gcggcgggta cccaaagact ctgaagactc
      121 cgtggtatcc tgagctatat catagttgat actatatatc cataaccgga aaatcaaggg
      181 cgacggggag gggaccccgt cgaaacgggg cagcgaccgc gccggccgcg gccttcggcg
      241 ggtcaggacg acgattcttc gtccctcttc cagagaacga acacgagcca cgccatgaag
      301 atcttcaccg gcagggcgat ctcctccggc agatcgatga ccgccttcct cgtgaaggcg
      361 ttttcgggcc tgatgacccc tatcgtccta tcgttctcgc tcaggatgaa cgtgcgtccc
      421 accggggacg cggcatgcag cctgtacgcc ctgcccttcg cggtgaccgt gaaggaccgg
      481 aacagggaac tcggcttttc ggcccgcgcc agttccatcc cgtcttcttc aaggatgaaa
      541 gcccctccga aaagaccttc acgaaagagt ctgtagctcc ttccgccaag gtgcaactcg
      601 ccggcctcac gcatccaccg gatgtcgatt ccggcgatta tctggtcgtt ttcgtcgaga
      661 atgttgaatt tccaggagaa ccaactcccg ggccgggctc tgagcatgag atggcggcat
      721 cctcagcagg gtatttcctc gcctttgcgc tgcgtccggg atcgcggacc gggaggattc
      781 ggttgcgcac ccatactttt atatatggtg gaaaaggaaa aggcaagagg gagcgtgaaa
      841 gacatgatga cgcccggtgt cggggatcgg tatttgacgt tttgcgggcg tggagctata
      901 gttggttaat tgttgcaggg gtgatgttca cagtacacaa aggaagggat catggaaagg
      961 gagcagagaa tagccgtcgc gtcagagatc atggagttgt ttgccgagct tacggggctt
     1021 gttcccggag gagtttctcc caggcggtac ctgtggacgg atgcgtacgg ggtctgcaac
     1081 tttctcggac tctatcggga gaccggggac gtcgcataca gggaccttgc gctgcggctg
     1141 gtggacgatg tgcatcatac actcggcagg caccgcaagg gcgacgcgag acagggttgg
     1201 atcagcgggc tggacgagga tggcggagag gaccatccga cggcgagagg cctgaggatc
     1261 ggcaagaaac tcggcgagcg cggccccgac gagcccctcg atgagaacgg ggaatgggac
     1321 agggatgggc agtactttca ctacctcacg aagtggatgc atgcgctcaa cctggtctcc
     1381 cgctcgacgg gcgacttcac atacaaccgg tgggcgatgg aactcgcgaa agccgcgcac
     1441 cgcgggtttg tctacaccgt tcccggaacg cggacgaggc ggatgttctg gaagatgagc
     1501 accgatcttt cctatcccct tgtctcctcc atggggcacc acgatccgct cgacggtctg
     1561 atcaccttta tccagctcga ggctatggag gaccccgaca ggccggaaca cctcggcctc
     1621 gcggccgaga tcga
//
LOCUS       DAOQTR010000517         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_101882_length_1626_cov_3.014640, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000517 DAOQTR010000000
VERSION     DAOQTR010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101882_length_1626_cov_3.014640"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..112
                     /gene="rpmF"
                     /locus_tag="PKN85_10530"
     CDS             11..112
                     /gene="rpmF"
                     /locus_tag="PKN85_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNT44893.1"
                     /translation="MACPNCKEPKLPHAVCPKCGMYKGKQYIAVEEA"
     gene            121..1131
                     /gene="plsX"
                     /locus_tag="PKN85_10535"
     CDS             121..1131
                     /gene="plsX"
                     /locus_tag="PKN85_10535"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNT44894.1"
                     /translation="MIRIAVDGMGGDFAPREIVRGAQSISREGLASIVLVGDEGKMRP
                     FVTDHANLDLVHTPVCVEMNEAPSNALRKKRDSSMNRAFELHKNGEVQAVVSAGNSGA
                     AMAFAIFTLGRIPGVDRPAIATLHPRMGEGVSILLDAGGTVDCKPTHLAQFALMGNAF
                     ASSVLGIASPRVGILSNGEEETKGNELTREAHALIKEFSLNYLGYVEGTDMYNGRTDV
                     VVSDGFVGNIALKISEGVADLIFEFFREGIKKSRKAQIGYFLLKDLFKEFQKKADYSE
                     TGGAPLLGVDGVCIICHGKSNEKAMRNAILLAKKFVEKGLKESVGEAMQDYESFQKVK
                     ER"
     gene            1135..>1626
                     /locus_tag="PKN85_10540"
     CDS             1135..>1626
                     /locus_tag="PKN85_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT44895.1"
                     /translation="MDYFLSEDQKSIQKLARRIAEEKVVPVRAELDETGQFPWEIMKH
                     CAETGLFGVSIPEEYGGMGGGSFENCIVVEELSKACLGVSVSYAASLLGAYPILLGGS
                     EEQKKKYLSQIASGSKLAAFGLTEANAGSDAQGIRTEAKKDGDHYILNGTKQWITNGG
                     EAEI"
BASE COUNT          379 a          450 c          518 g          279 t
ORIGIN      
        1 tgttgccgtc gtggcatgcc cgaactgcaa ggagcctaaa cttccccatg ccgtttgccc
       61 caagtgcgga atgtacaagg gcaaacagta catagcggtc gaggaagctt aaagcccgga
      121 atgatacgga tcgccgtcga cggaatgggt ggcgactttg cgcccaggga gatcgtgagg
      181 ggggcccagt cgatctcccg tgaggggctg gcttccatcg ttctcgtcgg cgacgaggga
      241 aagatgcggc ctttcgtgac ggaccatgcg aacctcgacc tggtccacac acctgtttgc
      301 gtggagatga acgaagcccc ttcgaacgcc ctcaggaaaa agagggactc ctccatgaac
      361 agggcattcg aactgcacaa gaacggagag gtccaggccg tcgtttcggc gggaaattcc
      421 ggggcggcca tggcctttgc catattcacc ctcggccgga tccccggcgt cgaccggccc
      481 gcgatagcca cgctgcaccc gcgcatggga gaaggggttt cgatccttct cgacgcgggc
      541 ggcaccgtgg actgcaagcc cacgcacctc gcccagttcg ccctgatggg caacgccttc
      601 gcctcttccg tcctggggat cgcttccccc cgggtgggca tcctttcgaa tggcgaggag
      661 gagacaaagg gcaacgaact gacccgggag gcccatgccc ttatcaagga gttcagcctc
      721 aactacctgg ggtacgtgga aggcaccgac atgtacaacg gcaggaccga tgtcgtggta
      781 agcgacggtt ttgtcggcaa catcgccctg aagatcagcg agggggtcgc cgacctgatc
      841 ttcgagttct tcagggaggg aataaagaag agccgaaagg cacagatagg ctatttcctt
      901 ctgaaagacc ttttcaagga attccagaag aaggcggatt attccgagac cgggggagca
      961 cctcttctcg gcgtcgacgg ggtctgcatc atatgccatg gaaaatcgaa cgaaaaggcc
     1021 atgcgcaacg ccatactgct ggcaaagaag ttcgtggaaa agggcctcaa ggaatccgtg
     1081 ggcgaggcca tgcaggacta cgaatcgttt cagaaagtaa aggagaggta aggtatggat
     1141 tattttctat ctgaagacca gaagagcata cagaagctgg cccgcaggat cgcggaggaa
     1201 aaggtcgttc ccgttcgcgc tgaactcgac gagacaggac agttcccctg ggagatcatg
     1261 aagcactgcg ccgaaacggg gctcttcggt gtcagcatcc ccgaggagta cggcggcatg
     1321 ggaggaggat ccttcgagaa ctgcatcgtc gtcgaggagc tgagcaaggc gtgcctcggc
     1381 gtgtcggtga gctacgcggc cagcctgctc ggtgcctacc ccatcctgct cggcgggtcg
     1441 gaggagcaga agaagaagta cctgtcccag atagcgagcg gatcgaagct ggccgccttc
     1501 ggactcaccg aggctaacgc cggcagcgac gcccagggca tcaggacgga ggcaaagaag
     1561 gacggcgacc attacatcct caacgggacg aaacagtgga taacgaacgg cggtgaggcc
     1621 gagatc
//
LOCUS       DAOQTR010000518         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_102020_length_1625_cov_2.566879, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000518 DAOQTR010000000
VERSION     DAOQTR010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102020_length_1625_cov_2.566879"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKN85_10545"
     CDS             <1..453
                     /locus_tag="PKN85_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44896.1"
                     /translation="AWPGIFPATMRRLPGRLRVTDVRAPSRGGVARMAVQFAAVWIAH
                     GIAFWVFCAAFAPLAWRTVLPVTGAYCLAYVSGLVALIAPGGIGVREEILGAMVDRLA
                     SQAEHEYETVIDKEKVFASIISDPETNRMENELFSKWYREGVNLLLKI"
     gene            555..845
                     /gene="groES"
                     /locus_tag="PKN85_10550"
     CDS             555..845
                     /gene="groES"
                     /locus_tag="PKN85_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNT44897.1"
                     /translation="MKVKPLQDRILVKRIEEEERTKGGIIIPDAAKEKPQEGKVVAVG
                     DGKILESGQKAPLTVKPGDKILFGKYSGTEIKVDGEEHLILREDDVLAIVED"
     gene            867..>1625
                     /gene="groEL"
                     /locus_tag="PKN85_10555"
     CDS             867..>1625
                     /gene="groEL"
                     /locus_tag="PKN85_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNT44898.1"
                     /translation="MAKEIKYDQNVREALLRGVNTLADAVRVTLGPKGRNVILEKTFG
                     SPTVTKDGVTVAKEVEVEDRFENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIYREG
                     AKLVAAGHNPMELKRGIEKAVEVIVGELKKLSKPTKDQKEIAQVGTISANNDDTIGNI
                     IAEAMSKVGKEGVITVEEAKSMETTLDIVEGMQFDKGYISPYFVTNPEKMEAILDEPF
                     ILINEKKISNMKDLLPILEQVAKMGKALLIVCEDV"
BASE COUNT          408 a          435 c          506 g          276 t
ORIGIN      
        1 gcgtggcccg ggatcttccc ggccacgatg cgccggctcc cgggacggct gcgcgtgacc
       61 gacgtgcgtg ccccgtcacg cgggggcgtg gcgcggatgg ccgtgcagtt cgccgcggtg
      121 tggatcgccc acgggatcgc gttctgggtg ttctgcgcgg cgttcgcgcc gctggcatgg
      181 cgcacggtgc tgcccgtgac cggcgcatac tgcctcgcgt acgtgtccgg gctcgtcgcg
      241 ctcatcgcgc ccggaggcat cggcgtgcgg gaggagatcc tcggcgccat ggtcgaccgt
      301 ctcgcctccc aggccgagca tgagtacgag accgtcatcg acaaggaaaa ggtcttcgcg
      361 tccatcattt ccgaccccga aaccaaccgg atggagaacg aactgttcag caagtggtac
      421 cgcgaagggg taaatttgtt gctaaaaatt taacgccccg gtattgacaa aaacttacca
      481 tcatccttat attgtctttg gcaatcgctt ttcgcaattg ctaacctaac aaccacagaa
      541 aggagtgtga aacaatgaag gtgaaaccat tacaggacag aattcttgtc aagagaatcg
      601 aggaagagga aaggacgaag ggcggtataa tcattcctga cgctgccaag gaaaaacccc
      661 aggaaggcaa ggtggttgcg gtgggcgacg gtaagatcct ggagagcggc cagaaggcac
      721 cgttgaccgt gaaaccgggg gacaagatcc tcttcggcaa atattcgggt acggagatca
      781 aggtcgacgg cgaagagcat ctcattctca gggaagatga tgttcttgca atcgtagaag
      841 attaataagg aaggagggat caaaggatgg ctaaggaaat caagtatgat cagaatgtca
      901 gggaggcgct cctgagaggc gtgaacacgc tggctgacgc cgtcagggtg actcttggac
      961 ctaaaggcag aaacgttatt cttgagaaga cgttcggttc tcccacggta accaaggacg
     1021 gcgtgacggt cgccaaagag gtcgaggttg aggacaggtt cgagaacatg ggtgcccaga
     1081 tggtgaagga agtcgccagc aagacgagcg acgtcgccgg cgacggcacc accacggcca
     1141 cggtcctggc ccagtcgatc taccgcgaag gagccaagct cgtagcggca ggccacaacc
     1201 ccatggaact gaagcggggc atcgagaagg cggtggaggt catcgtcggc gaactgaaga
     1261 agctgtcgaa gccgacgaag gaccagaagg agatcgccca ggtcggcacc atctccgcca
     1321 acaatgatga caccatcggc aacatcatcg ccgaggccat gagcaaggtc ggcaaggaag
     1381 gcgtcatcac cgtcgaggaa gcgaagagca tggaaacgac cctcgacatc gtcgaaggca
     1441 tgcagttcga caagggctac atctcgccct acttcgtcac gaacccggag aagatggaag
     1501 ccattctcga cgagcccttc atcctcatca acgagaagaa gatcagcaac atgaaagacc
     1561 ttctgccgat actcgagcag gtcgcgaaga tgggcaaggc cctcctcatc gtctgcgaag
     1621 acgtt
//
LOCUS       DAOQTR010000519         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_102112_length_1624_cov_3.157425, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000519 DAOQTR010000000
VERSION     DAOQTR010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102112_length_1624_cov_3.157425"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKN85_10560"
     CDS             <1..349
                     /locus_tag="PKN85_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT44899.1"
                     /translation="DLIIFSLKTDVEEITIAGTVFPDKMGRIVLLDRFHLDVIPEGTF
                     LAFKIMDRPGVIGKVGTILGDHGINIASFTVSRMVQGEEVAFVSVDSPIGKDVLKHIR
                     AIDGMIEAKEISL"
     gene            complement(500..>1624)
                     /locus_tag="PKN85_10565"
     CDS             complement(500..>1624)
                     /locus_tag="PKN85_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44900.1"
                     /translation="IDAVRELIGDMAHHFNNILNIIVGNAQLAKISPNCTEEMGSHLS
                     AIEEEVFRAADMVDALLASGSRHPMDMKTVDVGSVVRDFEKMVRKIIGDGISIVLSVS
                     STPIPAKMDVARIAQAFLNLVVNAREAMEGRGTLTIRVYAQDMTDVTTTLDGKIMPGP
                     YAVISVADTGRGMEKGVSEKIFEPFYTTDTSGEKKGLGLSVVLGIVKQHGGFVLCDSE
                     QGKGATFRIYLPISKEKTKVQRLEEQGAAGGTETVLVGEDEDNLRKIAANFLQTLGYR
                     VITARNGEEALALFHEVAGEIDMALLDVAMPGMNGFDVYHEIRKARPDIAVLFMTGYS
                     LDSANISTIRKQGISVIRKPFTMIALARRIREILDREKTA"
BASE COUNT          320 a          499 c          443 g          362 t
ORIGIN      
        1 cgatctaatc atcttcagcc tcaagaccga tgtcgaggag atcaccatag cggggacggt
       61 cttccccgac aagatgggca ggatcgtcct ccttgaccgg ttccacctcg acgtcattcc
      121 cgaggggaca ttccttgcct tcaagatcat ggaccgcccc ggcgtcatcg gcaaggtcgg
      181 caccatcctg ggcgaccacg gcatcaacat agcgagcttc accgtctccc ggatggtcca
      241 gggcgaggaa gtcgccttcg tctccgtgga cagccccatc ggcaaagacg tcctcaagca
      301 cataagggcc atagacggca tgatagaggc gaaggagata agcctctaga ggcggcttga
      361 accgcttgaa ccgcttgaac gggaaaagca aaaaacaata gagacaacag tacggcccgc
      421 cacccggatg acaaccggga gccgggccgt attcttatgc tctttcttcg tttcttaacg
      481 ttcaagcggt ttaagcggct caagccgtct tttccctgtc cagtatctcc cgtatcctcc
      541 gggcgagggc tatcattgtg aaaggttttc ggatgacgct tatgccctgc ttgcgtatgg
      601 tgcttatgtt tgcggagtcg aggctgtaac ccgtcatgaa aaggacggcg atgtcgggtc
      661 tcgcttttct gatctcgtgg tacacgtcga acccgttcat gccgggcatg gctacgtcca
      721 ggagcgccat gtctatctcc ccggccacct cgtgaaacag ggcaagggcc tcctcgccgt
      781 tcctggcggt gatgacccgg tatcccagcg tctgcaggaa attagcggcg atctttcgaa
      841 gattatcctc atcctcgcca acgaggaccg tctcggtgcc gccggcggcg ccctgctcct
      901 ccagcctctg taccttcgtc ttctccttcg agatgggcag atagatcctg aaagtcgcgc
      961 ctttaccttg ttcgctgtcg cacaagacaa agccgccgtg ctgtttgacg atcccgagga
     1021 caacgctcag gccgagtccc ttcttttcgc ccgacgtgtc agtcgtgtag aaaggttcga
     1081 atatcttctc gctcacaccc ttttccatgc cgcgcccggt atcggcgacg gatatcacgg
     1141 cataggggcc gggcatgatc ttcccgtcga gagtggtggt cacatccgtc atgtcctgcg
     1201 cgtaaacgcg gatcgtcagc gtccctctgc cctccatggc ctcccgagcg ttgaccacca
     1261 ggttgaggaa ggcctgggcg atccttgcca catccatctt tgcgggtatg ggtgtcgagg
     1321 aaacggacag gacgatgctg atcccgtcgc cgatgatctt ccgtaccatc ttttcgaaat
     1381 ccctcaccac ggacccgaca tccacggtct tcatgtccat gggatggcgg ctccccgatg
     1441 ccaaaagagc gtccaccata tccgcggccc tgaagacctc ctcctcgatg gccgaaagat
     1501 gcgagcccat ctcctcggtg cagttggggg atatcttagc aagctgggcg ttgcccacta
     1561 ttatgttgag gatattgttg aaatggtgcg ccatgtcgcc gatgagctcc cggaccgcgt
     1621 cgat
//
LOCUS       DAOQTR010000520         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_102144_length_1624_cov_2.570427, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000520 DAOQTR010000000
VERSION     DAOQTR010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102144_length_1624_cov_2.570427"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKN85_10570"
     CDS             complement(<1..221)
                     /locus_tag="PKN85_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44901.1"
                     /translation="MSTRDETIMTNKQIPITREADNNQEPGKRYRQSVVPLSLFNWLW
                     VNWALFSIGAGDGARTRGIKLGKLALYQL"
     gene            300..1031
                     /locus_tag="PKN85_10575"
     CDS             300..1031
                     /locus_tag="PKN85_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44902.1"
                     /translation="MKGKDQAKGKQPGLKAFWIKAGLVVAVGLIAIFRPDVPPAVFTF
                     PLIGPVKVYHIFWALAVFTLVKRMIPRFNKKISSRKIFGRFYRQAENITPKRDEQLRS
                     LKARTDRGALKSAFYWLLLLGDMALWRMVGMLSDTWIYIIVLFFIFMDQFCVTVFCPF
                     KWLAGGKCCSTCRINNWGYLMAFSPLIFVQSFWTWSIVALSIIVAIQWEYLYHRFPER
                     FYETHNAALMCRNCTVECTGHRKLR"
     gene            1118..>1624
                     /locus_tag="PKN85_10580"
     CDS             1118..>1624
                     /locus_tag="PKN85_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNT44903.1"
                     /translation="MSDQHEAIVIGGGPAGLTAGLYLMRGEIKAVLLEKVLPGGAPLN
                     TGRIENYPGFPEGISGRELMERFTTHAKAFGLPVREFAEVLSVTREEGLFTVTVNDGA
                     LTARDVIVASGTEPVRMGIPGENELVGRGISYCATCDGMFFRDLDVAVIGGGDSAIEE
                     ALSLANIVR"
BASE COUNT          320 a          473 c          464 g          367 t
ORIGIN      
        1 ctcagttggt agagtgcaag cttcccaagc ttgatgccgc gggttcgagc cccgtcgccc
       61 gctccaattg aaaacaatgc ccaatttacc cataaccaat tgaacaaaga gaggggcaca
      121 acggactgcc tgtatcgttt tcctggttct tggttattgt ctgcttctct cgttattgga
      181 atttgtttat tggtcattat cgtttcgtcc ctcgttgaca ttctgtcctt cctccagtta
      241 ttcttgcgga tgaaccgcgc ggaccccatt aaagcacgtg cctgtctccg ggtagacctg
      301 tgaagggcaa ggatcaagcg aagggcaaac aaccggggct caaggctttc tggataaagg
      361 ccggtctcgt cgtggcggtg ggtctcattg cgatcttccg ccccgatgtc ccaccggccg
      421 tcttcacctt tcctctcatc ggtcccgtga aggtatatca tatcttctgg gccctggccg
      481 tattcaccct cgtgaaaaga atgatccccc gtttcaacaa aaagatctct tcgcggaaga
      541 tattcggccg gttctacagg caggcggaga acatcacacc aaaaagggat gaacaacttc
      601 gctccctgaa ggcaaggacg gaccggggag ccctgaagtc ggccttctac tggctgctgc
      661 ttctcggcga catggcactg tggcgcatgg tgggcatgct gagcgacacc tggatctaca
      721 tcatcgttct cttcttcata ttcatggacc agttctgcgt gaccgtcttc tgtcccttca
      781 agtggctcgc gggcggcaag tgctgcagca cctgccgcat caacaactgg ggctacctca
      841 tggccttctc gcccctcatc ttcgtgcagt cattctggac gtggtccatc gtcgcccttt
      901 ccatcatcgt cgccattcaa tgggagtatc tctatcaccg tttccccgag cggttctatg
      961 aaacacacaa tgccgccctg atgtgcagga actgcaccgt ggagtgcacg gggcacagaa
     1021 aattacgttg aatattgcgg ggggattcct tacaatgaga gacacggcag gcggcctcgc
     1081 aggaggtcgc cgcggtcctt tcgggaggtt ttcgaatatg tcggatcagc acgaagcgat
     1141 agtcatcggc ggcgggcccg cggggctcac ggccggtctc tatcttatgc gaggcgagat
     1201 aaaggccgtc cttctcgaga aggtccttcc cggcggggcg cctctcaaca cggggcgcat
     1261 cgagaactat ccggggtttc ccgaaggcat atccggcagg gagttgatgg aaaggttcac
     1321 cacccacgcg aaggccttcg gccttcccgt cagggagttc gcggaggtcc tcagcgtcac
     1381 gcgcgaggag ggactcttca ccgtgacggt gaacgacgga gccctcacgg cccgggacgt
     1441 catagtggcc agcggcaccg aaccggtcag gatggggatc cccggcgaga atgagctcgt
     1501 gggcaggggc atctcatatt gcgccacctg cgacggcatg ttcttcaggg acctcgatgt
     1561 ggccgtcata ggcggcggcg attcggccat cgaagaggcc ctgtcgctcg ccaacatcgt
     1621 gagg
//
LOCUS       DAOQTR010000521         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_102217_length_1623_cov_3.308036, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000521 DAOQTR010000000
VERSION     DAOQTR010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102217_length_1623_cov_3.308036"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKN85_10585"
     CDS             complement(<1..211)
                     /locus_tag="PKN85_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44904.1"
                     /translation="MKVFPRSFILLSLVLCVACVPKVQQVREALPGPDLMKRQCELIG
                     QPRIERVSEHVWAAVGYDLANEILVH"
     gene            437..>1623
                     /locus_tag="PKN85_10590"
     CDS             437..>1623
                     /locus_tag="PKN85_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT44905.1"
                     /translation="MYRTFYGLRFKPFQILPNPDFFYMSPKHENAMTYLEYGIMERSG
                     FVLLTGEIGAGKSILIKYILGRLETTVMPAVVTNTNVTPDELLSMVLASFDIQSDSSS
                     KTDKLGALRRFLTGKSTRGRQALLIIDEAQNLSREALEEVRMLSNLVDADQMLLQIIL
                     VGQPEIRERLTRPELAQLNQRITVAYHLGALTEDETRQYIVFRLEKAGGRPDLFTHEA
                     MRMIFTASGGIPRTINILCDSALVYGFADETKKIGQDLVEQVIRDRGGMGLTVNREGG
                     EGRDNEMAAFPGCQELTGRVSAVEMGLADMKVKMQWLVGNLERTSQGFKDDLVLKMKE
                     LYTEERKRSDKLLFEFSRLKDRYTSLVSSREQREAEQKKIQYLAEDLENQLRLERERY
                     SSLL"
BASE COUNT          388 a          448 c          465 g          322 t
ORIGIN      
        1 tgtggacgag gatctcgttg gccaggtcgt acccgacggc ggcccagaca tgctctgaga
       61 cccgctcgat gcggggctgg ccgatgagct cgcactgcct cttcatgaga tccgggcccg
      121 gcagggcctc cctgacctgt tggaccttgg gcacgcaggc gacgcagaga accagggaca
      181 gcaagatgaa cgaccgggga aataccttca tgcaggcagc ctcctttacc gttacatggt
      241 atagaataac ttcatttgag cttcatggcc agggactcag aagaatcatc agggcaaaag
      301 gctgccctgg gcacttttga ccttctccag gtcttttcca ttgcaccttg gtgacagttg
      361 ggttaatgag aacctcgata tgtatcgtct gccttttaag gttgcactac aatacagata
      421 gaacgggaca gcgtccatgt acaggacctt ttacggatta cgtttcaaac ccttccagat
      481 attgcccaac cctgatttct tctacatgag ccccaagcat gaaaacgcca tgacctatct
      541 ggaatatgga atcatggaaa gatcgggatt cgtgctgctg accggcgaga tcggagcggg
      601 taaaagcatc ctcataaaat acatcctcgg ccgcctcgaa accacggtca tgccagcggt
      661 cgtgaccaac accaacgtga cgcccgacga gctgctctcc atggtgctcg cttccttcga
      721 tatacaatcg gatagttcat caaagacgga caagctcggc gcactgcgcc gctttctgac
      781 cggcaagagc acccggggca gacaggccct gctcattatc gatgaagcgc agaatctttc
      841 ccgggaagcg ctggaggagg tccgtatgct ctccaacctc gtcgatgctg accagatgct
      901 cctccagatc atcctcgtgg gccagcccga gatcagggaa cggcttacca gacccgaact
      961 cgcgcagctg aaccaacgca tcaccgttgc atatcacctc ggtgcactga ctgaggatga
     1021 gacaaggcaa tacattgtct ttcgcctcga gaaggcaggg gggcggccgg acctcttcac
     1081 gcacgaagcg atgcggatga tcttcacggc cagcggcggg ataccaagga ccatcaacat
     1141 actatgcgac agtgccttgg tgtatggatt cgccgacgaa acaaaaaaaa tcggccagga
     1201 tctggtggaa caggttatcc gggaccgtgg cgggatgggc ctcacggtga atcgtgaggg
     1261 gggggagggc cgagacaacg aaatggcggc gttccccggc tgccaggagc ttacaggtcg
     1321 tgtctctgcg gttgagatgg gcctcgcaga tatgaaggtg aagatgcagt ggctggtcgg
     1381 caacctggag agaacatctc agggcttcaa ggacgacctg gtcctgaaga tgaaggaact
     1441 ctacactgag gagcgcaagc ggagcgacaa gttgctcttc gaattctcac gtctcaagga
     1501 caggtatact tctctcgttt cctcgcggga gcaacgcgaa gcggagcaga agaaaattca
     1561 gtacctggct gaggacctcg agaaccagct ccgcctggag cgggagcggt acagctccct
     1621 gct
//
LOCUS       DAOQTR010000522         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_102724_length_1618_cov_3.317978, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000522 DAOQTR010000000
VERSION     DAOQTR010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102724_length_1618_cov_3.317978"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..>1618
                     /locus_tag="PKN85_10595"
     CDS             290..>1618
                     /locus_tag="PKN85_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT44906.1"
                     /translation="MGDQENREAKELSLLFDISTRLSGSLDLKTVLKPILHLIAGHME
                     IPRGTLTILNRARGEIAIEEAYGLRPDEQARGRYRMGEGITGRVIDTGQPVVVPNIAD
                     EPLFLDRTGSRRNVNKRDVAFICVPITIGSEVIGALSADRLHNEGMSLENDVRLLTII
                     ASTISQAVRLRQLGQEELEKMKDENRRLQDQLKTRYDMKTIVGNSKGMRAVYSLIDKV
                     CRTGATVLILGESGVGKERVAQTIHFSSNRASRPFVKVNCAALPESLIESELFGHERG
                     SFTGATMARKGRFETAGDGTIFLDEVGDLPPLVQVKLLRVLQEKEFERVGGNAPIKLE
                     ARIITATNKDLQTLVREGKFREDLYYRFNVFPILVPPLRDRKTDIMLLADHFIQKYGR
                     EHGKEITSVSTTSTDMLMNYHWPGNVRELENCIERAIILCTDGVIHSCHLP"
BASE COUNT          365 a          469 c          493 g          291 t
ORIGIN      
        1 ctcgtacaac cccaacgggc ccgtgaacaa caacttcgtc tacggtatcg ggctgacata
       61 tgctttctag atcgaattga ccgtcgtata gtcgtgcaag actcgttgac aatgatcccg
      121 atgttatata gaataaccct gtcacagtga tgtgacggac aatggcgttc acagggaacg
      181 ccatttctat tttgggacga aggcgtcttt gtccgcccca gggggggcgc gcgaagacgc
      241 ctttttctat tgggaacacg agccggccgg gccggaaagg gggccacaca tgggcgacca
      301 ggaaaatcgc gaggcaaagg agctttcgct tctcttcgat atcagcacca ggctgagcgg
      361 gagccttgat ctcaagacgg tcttgaaacc gatcctccac cttattgccg ggcatatgga
      421 gataccccgg ggtaccctca ccatcctcaa ccgggcgagg ggagagatcg cgatagagga
      481 ggcatacggt cttcgccccg acgagcaggc gaggggcagg taccgcatgg gtgaaggcat
      541 cacggggagg gtgatagata cgggccaacc cgtcgtggtt cccaatatcg ccgatgagcc
      601 cctgttcctc gacaggaccg ggtcgaggag gaatgtcaac aagagggatg tcgccttcat
      661 ctgtgttccc atcacgatag ggagcgaagt gatcggcgcg ctgtccgccg acaggctgca
      721 caacgaaggc atgtccctcg agaacgacgt gcgcctcctg accatcatcg cgtccaccat
      781 ctcccaggcc gtccgccttc gccagctcgg tcaggaggag cttgagaaga tgaaggacga
      841 gaaccggcgc ctgcaggacc aactgaagac gcggtacgac atgaagacca tcgtcggcaa
      901 ctcgaagggt atgcgcgccg tgtattccct catcgacaag gtgtgccgga ccggcgcgac
      961 ggtgctcatc ctgggagaga gcggcgtcgg taaggagcgt gtggcgcaga ccatccactt
     1021 cagttcgaac agggcctccc ggccctttgt gaaagtgaac tgcgcggccc tgcccgaatc
     1081 cctgatcgag agcgagcttt tcggtcatga aaggggttcg ttcacgggcg cgacgatggc
     1141 gcgcaagggc cggttcgaga cggccgggga cggcaccatc tttctcgacg aggtgggcga
     1201 tctgccgccc ctggtccagg tgaagctgct ccgcgtgctg caggaaaaag agttcgaacg
     1261 cgtcggcggc aacgcgccca taaagctgga agcgaggatc atcacggcga cgaacaagga
     1321 ccttcagacc ctggtccgtg agggaaagtt cagagaagac ctctactaca ggttcaacgt
     1381 ctttcccatc ctcgtcccgc cgcttcggga ccgtaagacc gacatcatgc tcctcgccga
     1441 ccatttcatt cagaaatacg gcagagagca cggaaaggag atcacgagtg tgtccacgac
     1501 ctcgacggat atgctcatga actaccactg gccgggcaac gtccgggagc tggagaactg
     1561 tatcgaaagg gcgataatcc tctgcacgga cggcgtcatc cacagctgcc acctcccg
//
LOCUS       DAOQTR010000523         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_103129_length_1614_cov_2.893521, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000523 DAOQTR010000000
VERSION     DAOQTR010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103129_length_1614_cov_2.893521"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PKN85_10600"
     CDS             complement(<1..550)
                     /locus_tag="PKN85_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT44907.1"
                     /translation="MTSKSLSGIDLLHLTIDDIRRIFSVDDRGLLEEMAHAARELTRR
                     QFGRTISLYAPLYIANYCENECVYCGFHASQKSMPRSKLTMEQIDRECEALAATGNRS
                     VLILTGESRFHSSPQYIRDAVRIASRYFSYVALEVYPLDEEEYRELYLAGVDGVTLYQ
                     ETYDRARYDELHLAGPKKVYDYR"
     gene            complement(540..1316)
                     /locus_tag="PKN85_10605"
     CDS             complement(540..1316)
                     /locus_tag="PKN85_10605"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNT44908.1"
                     /translation="MDDKLIVAGREFGSRFFLGTGKFGDKDAMRKAIASSGSQLVTVA
                     LRRIDLDETDENILSFIPEGVTIMVNTSGARNAEEAVRIAHIAREAGYGDWIKIEVIN
                     DSRYLLPDNQETIKATKVLSGEGFVVLPYMHPDLYVAKALVDAGAAAVMPLGSLIGSN
                     QGLKMRTLIEVLIEEVMEVPVVVDAGIGRPSHAAEAMEMGADAVLANTAVADAEDPPL
                     MAAAFARGVEAGRMAYLAKLGKERKGAAASSPLTGFLYDE"
     gene            complement(1319..1519)
                     /gene="thiS"
                     /locus_tag="PKN85_10610"
     CDS             complement(1319..1519)
                     /gene="thiS"
                     /locus_tag="PKN85_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNT44909.1"
                     /translation="MKITVNGRTMEIAEGMDLHRLVHSGKADPGRVILVLNDGVVKSD
                     RWSLTVLSEGDRVELVSFVGGG"
BASE COUNT          282 a          537 c          462 g          333 t
ORIGIN      
        1 cccggtaatc gtagaccttc ttgggacccg cgagatgaag ctcgtcgtag cgggccctgt
       61 cgtacgtctc ctggtacagc gtaaccccgt ccactccggc aaggtagagc tccctgtatt
      121 cctcctcgtc gagaggatag acctccaggg cgacgtagga gaaataacgc gacgctatcc
      181 tgacggcgtc gcggatatac tgcggagaag agtggaaacg ggattcaccc gtcaggatga
      241 ggacgctgcg gttccctgtg gcggcgagag cctcgcactc cctgtctatc tgttccatgg
      301 tgagcttgct ccggggcata ctcttctgag aggcatggaa tccgcagtag acgcattcgt
      361 tctcgcagta gttcgctatg tagagcgggg cgtagaggct gatcgtccga ccgaactggc
      421 gcctcgtcaa ctcgcgcgcg gcatgggcca tctcctcgag caggcccctg tcatccaccg
      481 agaatatgcg gcggatatca tcgatggtca ggtgcaggag gtctatcccg ctgaggctct
      541 tactcgtcat agaggaaccc cgtcagcggt gaggaagcgg ccgctccctt tctctccttc
      601 cccagctttg ccaggtatgc catcctgccc gcctcgacgc ctcgcgcgaa agcggcggcc
      661 atgagcggcg ggtcctcggc gtcggcgacg gccgtgttgg cgagcaccgc gtcggccccc
      721 atctccattg cctcggccgc atgcgatgga cgtcctatgc cggcgtcgac gactacgggg
      781 acctccatga cctcctcgat cagcacctct atgagggtac gcatcttcag cccctggttc
      841 gatccgatga gggacccgag aggcatgacg gctgccgccc ccgcgtcgac gagcgccttc
      901 gccacgtaga ggtcgggatg catgtagggc agcacgacga agccttcccc cgagaggacc
      961 ttcgttgcct ttatcgtctc ctggttgtcg ggcagcaggt accggctgtc gtttatgacc
     1021 tcgatcttga tccagtcccc gtatcccgcc tcccgcgcta tgtgtgcgat gcgcacggcc
     1081 tcctccgcgt tgcgcgcgcc cgacgtgttg accatgatcg tcacgccctc gggaatgaag
     1141 gagaggatgt tctcatccgt ttcgtcgagg tcgatccggc gaagcgcgac cgttacaagc
     1201 tgagagccgg agctcgcgat cgccttgcgc atggcgtcct tgtcgccgaa ctttccagtg
     1261 ccgaggaaga agcggctccc gaactccctt ccggccacga tcagtttgtc atccataatc
     1321 accctcctcc cacgaaggac accagttcaa cccggtcacc ctcagaaagg acggtcaggg
     1381 accaccggtc gctcttcacg acgccatcat tgaggacaag gatcaccctg ccggggtccg
     1441 ccttgccgga atggacaagc ctgtggaggt ccataccttc cgcgatctcc atcgtcctgc
     1501 cgttgactgt tatcttcatt tcgctgcctt ccgtattctc ttcagcaact ccaccgcgtc
     1561 cgatgaggcc ctgacaacac ctatcgtctt cgcccccgcg gccagcacgt catc
//
LOCUS       DAOQTR010000524         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_103245_length_1613_cov_2.756739, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000524 DAOQTR010000000
VERSION     DAOQTR010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103245_length_1613_cov_2.756739"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="proS"
                     /locus_tag="PKN85_10615"
     CDS             complement(<1..974)
                     /gene="proS"
                     /locus_tag="PKN85_10615"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNT44910.1"
                     /translation="MAEEDQGALPPKGDFSAWYNELLWRAEVMDVRYPVKGLYVWYPF
                     GFSIRRNVYDMLRRLLDRDHQEALFPLLIPENELAKEAEHIKGFEDEVYWVTHGGLTP
                     LDVKLALRPTSECAIYPMYALWVRSHADLPLKIYQIVNTFRYETKQTRPMIRAREITS
                     FMESHTVHATWDEAETQVEQEIALTLEFYDSLCIPLIISKRPDWDKFPGADYTVAVDT
                     VMPNGRTLQIGTVHHLGTHFSKTFGISFEDEKGAQKPANQTCYGISERSVAALIGMHG
                     DDRGLVLPPGPAPVQVVIIPITIGKRKDEVGAAAAGIEKELSAAGFRV"
     gene            complement(985..1545)
                     /locus_tag="PKN85_10620"
     CDS             complement(985..1545)
                     /locus_tag="PKN85_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNT44911.1"
                     /translation="MSDLSRNAWIVAILLAVSVAFGAWATIQDPAVGEQLISLLKESI
                     MGEALDSSGAMMAVMLFFNNLQACVLMFIGGASLGLLTVFIISTNGFVIGSVLEMVRE
                     EHSALYVAAAIIPHGIFEIPAFVVSGALGLMLAKALWKEWEGSEDAASRALAYARTFL
                     LAVVPLVAVAACVEAFITPEILRLAI"
BASE COUNT          316 a          502 c          498 g          297 t
ORIGIN      
        1 ttcacccgga accccgctgc agagagctcc ttctcgattc ccgccgctgc cgcgcccacc
       61 tcgtctttcc gctttcctat cgtaatggga atgatcacga cctggacagg tgcggggccc
      121 ggcggaagga ctagtccccg gtcgtcgccg tgcatgccga taagggcagc gaccgaccgt
      181 tctgagatgc cgtagcaggt ctggttcgca ggcttctgcg ctcccttctc atcctcgaaa
      241 ctgatgccaa acgtctttga gaagtgcgtg ccgaggtgat ggacggtgcc gatctggagc
      301 gtccttccgt tcggcatcac cgtgtcgacc gcgaccgtgt agtccgcgcc ggggaacttg
      361 tcccagtccg gccgcttcga gatgatgagg gggatgcaga gcgaatcgta gaactcgagg
      421 gtgagcgcga tctcctgttc gacctgcgtt tccgcctcgt cccatgtcgc gtggacggtg
      481 tgcgactcca tgaacgaggt gatctcccgg gcgcggatca tcggccgggt ctgcttggtc
      541 tcgtaccgga acgtgttgac gatctggtag atcttcaggg gaaggtcggc gtgggagcgg
      601 acccagagcg cgtacatggg ataaatggcg cactcgctcg tcggccggag cgcaagtttc
      661 acgtcgagcg gggtgaggcc gccgtgggtg acccagtaga cctcgtcctc gaatcccttg
      721 atgtgttcgg cctccttcgc aagctcgttc tcggggatga gcagggggaa gagggcctcc
      781 tggtggtcac ggtcgagcag cctgcgcagc atatcgtaga cgtttcgcct gatcgagaac
      841 ccgaacgggt accagacata gaggcccttg accgggtacc ggacgtccat gacctccgcc
      901 cgccacagga gctcgttgta ccatgcagag aagtcaccct tgggggggag cgccccctga
      961 tcttcctcgg ccaaaaaaca cacctcaaat tgctaaacgt aggatttcag gcgtaataaa
     1021 tgcctctacg cacgcggcaa ccgcgacgag ggggacgact gccagcagga acgtgcgggc
     1081 ataggcaagc gcccgggaag cagcgtcctc gcttccttcc cattccttcc agagcgcctt
     1141 tgccagcatg agcccgagcg ccccggatac cacgaacgcc gggatctcga agatgccatg
     1201 ggggatgatc gcggccgcca cgtaaagggc gctgtgctcc tccctcacca tctcgaggac
     1261 agacccaatc acaaagccgt tcgtgctgat gatgaagacc gtgagcaggc cgagcgacgc
     1321 cccgccgatg aacatgagga cgcaggcctg gaggttgttg aagaagagca tcaccgccat
     1381 catcgccccc gatgaatcga gcgcctctcc catgatggac tccttgagga gcgagatcag
     1441 ctgctccccc accgcggggt cctgaatcgt cgcccatgcc ccgaacgcga ccgacaccgc
     1501 caggaggatt gcgacaatcc aggcgttcct ggaaaggtca gacataaagg accatcctca
     1561 tcatgtcccg gattcccggc gcaagtccga cagtgatcat ggcgaggagg atg
//
LOCUS       DAOQTR010000525         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_104984_length_1597_cov_2.831388, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000525 DAOQTR010000000
VERSION     DAOQTR010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104984_length_1597_cov_2.831388"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="PKN85_10625"
     CDS             complement(<1..1550)
                     /locus_tag="PKN85_10625"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNT44912.1"
                     /translation="MRCEFIGDQILSLRHFNPLSQRSDKELARCDITSIIKKDGGGSS
                     TIIDHLGGPLTLVHPGDSLIDHALHGADAATKENWARLLAEAPQIDLSGVVGPSETQK
                     TLRAVSNDDLRQLFLLHKTRIFHILREKLQGEWSHYPYVYLFANTAHQAKRLQEIFES
                     YGLVLPILPRISYRRRSREWGIIEGPIRRGFRTDDIIVLTEDDIAGPKKRVVKRHWGS
                     RDEFLASFKDLTEGELVVHVDHGIGVYRGIVPLTVNGFTKDFLLIEYLGGDKLYVPVD
                     DLHLVQKFIGSEKFKPKIDRLGSRFWLNTKKRVRRQIEDIAGELLEIYAARQMAEGHS
                     YPPEDELFREMESRFEYEETEGQINAINDVLADLRSTRPMDRLVCGDVGFGKTEVALR
                     AAFKAVLDGKQVAVLVPTTILAQQHFKTFSDRLSDYPIVMGVLSRFRTKDEQAETARL
                     LEKGQVDIVIGTHRLLQKDVSFRDLGLLIVDEEHRFGVRHKEKLKAIRKTVDVLTLSA
                     TPIPRTLY"
BASE COUNT          276 a          452 c          512 g          357 t
ORIGIN      
        1 atgtagaggg tcctcgggat gggcgtggcg ctcaaggtca gaacatccac ggtcttcctg
       61 atggccttga gcttttcctt gtgtctcaca ccgaagcggt gctcctcgtc gacgatgaga
      121 agaccgaggt cgcggaagga aacgtccttc tgcaggagcc tgtgggtgcc gatgacgatg
      181 tccacctgcc ccttctcgag gagccttgcc gtttccgcct gttcgtcctt cgtccggaag
      241 cgcgacagca cgcccatgac gatggggtaa tcggagagac ggtcgctgaa ggtcttgaag
      301 tgttgctggg cgaggatggt ggtgggcacg agcacggcca cctgttttcc gtcgaggacg
      361 gccttgaagg ccgctctgag agcgacttcc gtcttgccga agccgacatc accgcagacg
      421 agcctgtcca tgggtctcgt gcttctcagg tccgcgagga catcgtttat ggcattgatc
      481 tggccctccg tctcctcgta ctcgaagcgg gactccatct ccctgaagag ctcgtcctcg
      541 ggcggatagc tgtggccctc cgccatctgc ctcgccgcgt agatctcgag aagctcgccg
      601 gcgatgtcct cgatctgcct gcgaacccgt ttcttcgtgt tgagccagaa cctcgagccg
      661 agccggtcga tcttgggctt gaacttctcg ctgccgatga acttctgcac gaggtgcaga
      721 tcgtcgacgg ggacatagag cttgtctccg cccaggtatt ctataaggag gaagtctttc
      781 gtgaacccgt tgacggtcag cgggacgatg ccgcgataca cgccgatgcc gtggtccaca
      841 tgcacgacga gctcgccctc ggtgaggtct ttgaaagaag cgaggaactc atcccggctg
      901 ccccagtgtc ttttgacgac cctcttcttg ggtccggcta tgtcgtcctc ggtgaggacg
      961 atgatgtcgt cggtcctgaa gcccctgcgg atgggccctt cgatgatgcc ccattccctg
     1021 gagcgtctcc ggtaggatat gcgcgggagg atgggcagaa ccaggccata gctctcgaat
     1081 atctcctgga gcctctttgc ctggtgcgcc gtgttggcga agaggtagac gtaggggtag
     1141 tgggaccact ccccctgcag tttttcccgg agtatatgga atatgcgggt cttgtggaga
     1201 aggaagagtt gccgcaggtc atcgttggag acggccctga gggtcttttg ggtctccgac
     1261 gggccgacaa cgcccgaaag gtcgatctgc ggcgcttcgg cgagaagccg cgcccagttt
     1321 tccttcgtcg ccgcgtcggc gccgtgaagc gcgtggtcga tcagggagtc tcccgggtga
     1381 acgagggtga gaggtccgcc gaggtggtcg attatggtgg atgacccgcc gccgtccttc
     1441 tttatgatgg acgttatatc acagcgggcg agctccttgt cggacctctg ggaaagaggg
     1501 ttgaaatgtc tcagggagag tatctggtct cctatgaact cgcatctcac cggcctgtcc
     1561 gaggcgggag ggaaaaaatc gatgatgctg ccccgtt
//
LOCUS       DAOQTR010000526         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_105507_length_1592_cov_3.093689, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000526 DAOQTR010000000
VERSION     DAOQTR010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105507_length_1592_cov_3.093689"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1205)
                     /locus_tag="PKN85_10630"
     CDS             complement(9..1205)
                     /locus_tag="PKN85_10630"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNT44913.1"
                     /translation="MMDELLLSNDNKVIFLILDGLGDIPNPDFGLMTPLEAAAKPNMD
                     HLATKRGVLGRIIPVGTGITPGSGPGHLSLFGYDPVASEIGRGVLEVLGLNMDLRNGD
                     LAARANFCTIREDVVTDRRADRIPTEETERLCAMISASVKDIEGVEVIIRPGKSHRFA
                     AIFRGAGLSDKLTDADPHKDNKPFAYTTEKAKGAEFAARVVNGFIDRVRDLLKAEPVA
                     NGALLRGFSQKPDITPFAKRYRMKALAIATYPMYRGIAKVLGMDVKEEPGSYEEAVDI
                     LKKNYNDYQFFFFHVKETDLAGEDGNFKEKVKAIEDVDRFLPGISALDPQALVVTGDH
                     STPCPLKGHSWHPVPLLIVTKTGEIDGLAFHEKNCIVGSAGIIYSKELMFLALAHGGR
                     LDKYGA"
     gene            complement(1202..>1592)
                     /locus_tag="PKN85_10635"
     CDS             complement(1202..>1592)
                     /locus_tag="PKN85_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44914.1"
                     /translation="EDHLISFLYARLCLRLEMIDEAIDTLNTLIAEGEDFKGLHRAMA
                     EAYIHRGELENAVEEFRGAFPIEQQVYIPFVCTKCQAIKEEWSDFCESCYSWNTINVK
                     KEEFLHADMNELRMLYDEAEWTRGEPS"
BASE COUNT          290 a          475 c          460 g          367 t
ORIGIN      
        1 tgaaaaggct aagcaccgta cttgtcgagc cgccctccgt gggcaagggc tagaaacatg
       61 agttccttac tgtatatgat tcccgcgctc ccgacaatgc agttcttctc gtgaaaggca
      121 agtccgtcga tctcacccgt tttcgtaaca atgagaaggg gaacgggatg ccagctgtgt
      181 cctttaagcg gacagggcgt cgagtggtcg cccgttacga cgagcgcctg gggatcgagg
      241 gccgatatcc cgggaaggaa cctgtccacg tcctcgatcg ccttcacttt ctctttgaaa
      301 ttgccgtcct cgccggcaag gtccgtctcc ttcacgtgga agaaaaagaa ctggtagtcg
      361 ttgtagttct tcttcaggat gtccacggcc tcctcgtaac tgcccggctc ctccttgaca
      421 tccataccga ggaccttcgc gatcccccgg tacatcgggt acgtcgcgat ggcgagcgcc
      481 ttcatcctgt atctcttcgc gaagggtgtt atgtccggtt tctgggagaa accgcggagc
      541 agcgccccgt tggccaccgg ctcggccttg agcaggtccc tgaccctgtc gatgaaaccg
      601 ttcaccacac gggcggcgaa ttccgcgcct ttcgccttct cagtggtata tgcgaaaggc
      661 ttgttgtcct tgtgggggtc ggcgtcggta agcttgtcgc tcaaacccgc gcccctgaat
      721 atggcggcaa accggtggga cttccccggt ctgatgatga cctcgacgcc ttcgatgtct
      781 ttcaccgagg cgctgatcat ggcgcagagc ctttccgtct cctccgtcgg gatcctgtcc
      841 gccctgcggt cggtcaccac gtcttccctg atggtgcaga agttggcccg tgccgcaagg
      901 tccccgttac gcaggtccat gttgagaccg aggacttcga gaacgccgcg gccgatctcg
      961 ctggccacgg gatcgtaccc gaagaggctg aggtgccccg gaccgctgcc gggcgtgata
     1021 cccgtgccga cgggtatgat cctgccgagc acgccccttt tcgtggcaag atggtccatg
     1081 ttcggcttgg cagcggcttc gagaggggtc ataagaccga aatcggggtt cgggatatct
     1141 ccgagaccgt caagtatgag aaaaatgacc ttgttgtcgt tcgataagag aagctcgtcc
     1201 atcatgatgg ttcacctcgg gtccattccg cctcatcgta gagcatgcgc agttcgttca
     1261 tgtcggcgtg gaggaattcc tccttcttca catttatcgt gttccagctg taacagctct
     1321 cgcagaagtc gctccactcc tcctttatgg cctgacactt ggtacagacg aaaggtatgt
     1381 agacctgctg ctctatcggg aaggcgcccc ggaactcctc gacggcgttc tccagttctc
     1441 cccggtggat ataggcctct gccatcgccc ggtgaagacc cttgaagtct tcgccctcgg
     1501 ctatgagggt gttgagggta tcgatcgcct cgtcgatcat ctcgaggcgc aggcagagcc
     1561 gcgcgtagag aaaggagatc aggtggtcct cg
//
LOCUS       DAOQTR010000527         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_105549_length_1592_cov_2.405335, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000527 DAOQTR010000000
VERSION     DAOQTR010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105549_length_1592_cov_2.405335"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PKN85_10640"
     CDS             <1..1356
                     /locus_tag="PKN85_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44915.1"
                     /translation="GKTWFRKAYPDEAYRHRVVEAWLEDLRTKSLRDQGIYNVVCKDG
                     STKVISFIPVQLGNGDVIMVCEDITERKRLEAQLINAQKMEAIGTLSGGIAHDFNNIL
                     MGIQGYISLMMLDAGAGHPHREWLGNIEELVRNAADLTGQLLGFARGGKYNVKPLSMN
                     ELVEKTSAMFGRTKKEIIIRHGYQTDVWPVEGDQGQIEQVLLNLYLNAWQAMPAGGVL
                     SLGTRNTVIASAEADVHSVTPGRYVCTSITDSGVGMDVKTRERIFEPFFTTKELGRGT
                     GLGLAMVYGIVRNHNGYIDVESEPGKGSTFRFHLPASERDVARIRPETAETVKGVGTV
                     LLVDDEPAVLTVSKAMIESLGYTVHAVGNGEKAIALYREGKDSIDLIVLDMIMPGLSG
                     GETFNRLREMDPGARVILSSGYSLNEQARGIMEKGCRGFVQKPFTIADISRKLREVLE
                     T"
     gene            complement(1397..>1592)
                     /locus_tag="PKN85_10645"
     CDS             complement(1397..>1592)
                     /locus_tag="PKN85_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44916.1"
                     /translation="RKPRRALHPLARSGKLTPVPPADAGSLVLSSREAGCKCAQFLEG
                     SGNWARLFSACSPRNLRALE"
BASE COUNT          357 a          448 c          519 g          268 t
ORIGIN      
        1 gggaagacgt ggttcagaaa ggcatatccc gatgaggcct accggcaccg tgtcgtagag
       61 gcatggcttg aggacctcag gacgaagagc ctcagggacc agggcatcta caatgtcgtg
      121 tgcaaggatg gctcgacgaa agtgatcagc ttcatccccg tccagctcgg caacggcgac
      181 gtgatcatgg tgtgcgagga catcaccgag cgcaagaggc tggaggccca gctcatcaac
      241 gcccagaaga tggaggcgat aggtaccctc tccggtggta tcgcccatga tttcaacaac
      301 atactcatgg gtatccaggg ttacatatcg ctcatgatgt tggacgcggg cgcgggccat
      361 ccccatcgcg agtggctcgg gaacatcgag gaactcgtaa ggaacgcggc ggacctgacg
      421 ggacaactcc tcgggttcgc gcggggcggc aagtacaacg tcaaaccgct cagtatgaac
      481 gaactcgtcg agaagacctc cgcgatgttc ggaaggacga agaaggagat catcatccgc
      541 cacgggtacc agacggacgt ctggcccgtc gagggagatc agggacagat agaacaggtc
      601 cttctcaacc tttacctcaa cgcctggcag gccatgcccg ccggcggggt cctctcgctg
      661 gggacgagga acaccgtcat tgcgtccgcg gaggcggatg tccattccgt gacgcccggg
      721 agatacgtgt gtacctctat caccgacagc ggcgtcggca tggacgtgaa gacgagggag
      781 aggatcttcg agcccttctt tacaacgaag gagcttggca ggggaacggg actgggtctc
      841 gccatggtgt acggcattgt aaggaaccac aacgggtaca tcgatgtgga gagcgaaccc
      901 ggcaaagggt ccacattccg gttccatctt cccgcatcgg aaagggatgt ggcccgtatc
      961 aggcccgaga ccgccgaaac ggtaaaaggc gtgggaacgg tcctgcttgt cgatgacgaa
     1021 ccggcggtcc tcaccgtcag caaggccatg atcgagtccc tgggatacac ggtgcatgcc
     1081 gtcgggaacg gggagaaggc catagctctc tacagggaag ggaaggattc catcgatctc
     1141 atcgtccttg acatgatcat gcccggcctg tcgggagggg agaccttcaa ccgcctgcgg
     1201 gagatggacc ccggagcgag ggtgatcctc tcgagcggct acagccttaa cgagcaggcg
     1261 cggggtatca tggaaaaggg ttgcagggga ttcgtccaga aacccttcac catagccgac
     1321 atctcccgca agctccggga agtactggag acctgagaga ccttgtctca cgcccgttcc
     1381 ccggccggag gcggggtcac tcgagggcac ggaggttgcg tggagagcac gcagagaaaa
     1441 gacgcgccca gttcccggac ccctccagga actgggcgca tttgcatccc gcttcgcggg
     1501 aagagagaac aagtgatccc gcgtccgccg ggggaacggg cgtgagcttt cctgaccttg
     1561 ctagcggatg aagtgcgcgc cgcggcttac gt
//
LOCUS       DAOQTR010000528         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_105967_length_1588_cov_3.071102, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000528 DAOQTR010000000
VERSION     DAOQTR010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105967_length_1588_cov_3.071102"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PKN85_10650"
     CDS             complement(<1..1228)
                     /locus_tag="PKN85_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNT44917.1"
                     /translation="MKVITSHMNADFDSLSSMVAARKLYPDATLVFPGSQEKTLRDFL
                     IHSTLYLFDIAKLKEIDHRSVDTLVLVDTRDKTRIGDLAKVADDPKVAIHVYDHHPDS
                     GNDVRADVQVVRSVGATVTILVSMIRERGISLTPEEATIMMLGIYEETGSFRFSSTTV
                     EDFEAASYLLSQGANVNLVSDMLVRELTPEQVFLLNDIIKNATVYNINGIDIVITEGS
                     TEQYVGDLAVIVHKYRDMENINAVFALFRMEDRIHIIGRSRIPEVDSGYVMSLLGGGG
                     HKVAASSTVKEMTLPEAKERLIEILRNNVKPLWKAKDIMFFPVKSVDSQSAIGEALSV
                     LTKYNINAMPVLSRGKVVGVITRQVAAKALFHKLAEQPVEGRIGRGDGPDVLGPVQFA
                     GPVFVVAVEANGDLAAV"
     gene            complement(1228..1566)
                     /locus_tag="PKN85_10655"
     CDS             complement(1228..1566)
                     /locus_tag="PKN85_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNT44918.1"
                     /translation="MPLGSYSRDAVKAYLDEERPQGQYLFPNRRGGMITRQAVWKIIR
                     KYGLKLSRGKVSPHTIRHTFATHLLEGGADLRSVQVLLGHEDISTTQIYTHVDSKRLK
                     EIHKKHHPRG"
BASE COUNT          302 a          475 c          460 g          351 t
ORIGIN      
        1 cgacggcggc caggtcaccg ttcgcctcca cggcaacgac gaagaccggg cccgcaaact
       61 gaaccggacc gagaacgtcc ggcccatccc cccgaccgat ccggccttcg acgggctgtt
      121 ccgccagctt gtggaacagt gccttcgccg ccacctgccg cgtgatgacc cccaccacct
      181 tgccgcgtga aagcacgggc atggcgttta tgttgtactt cgtaaggacg ctcagggctt
      241 cgccaatggc gctttgggaa tcgacggact tgacggggaa gaacatgatg tcctttgcct
      301 tccagagagg cttgacgttg ttgcgaagga tctcgatgag cctttccttc gcctcgggaa
      361 gcgtcatctc ctttaccgtg gacgacgctg cgaccttgtg gcccccgccg ccgagaaggg
      421 acatgacgta ccccgaatcc acctccggta tcctgctgcg cccgatgatg tggatccggt
      481 cctccatccg gaacagggca aagacggcat tgatgttctc catgtccctg tacttgtgga
      541 cgattacggc aaggtccccc acgtactgct ccgtgcttcc ctcggtgatg acgatatcga
      601 tgccgttgat attatagacc gtggcattct tgatgatgtc attgagaagg aagacctgct
      661 ccggagtcag ctcccggaca agcatgtcgg atacgagatt gacgttcgct ccctgggaaa
      721 ggagatagga cgcggcctcg aagtcctcga cggtggtgga ggaaaacctg aaactgcccg
      781 tctcctcata tatgcccagc atcattatcg tcgcctcctc gggcgtgaga cttatgcccc
      841 tctcccttat catggagaca aggatggtca ccgtcgcacc gacgctcctg accacctgca
      901 catccgccct cacatcgttg cccgaatcgg gatggtggtc atatacgtgt atcgcgacct
      961 tcggatcgtc cgccaccttc gccaggtcgc cgatgcgcgt cttgtcccgc gtgtcgacaa
     1021 ggacgagggt gtcgacggaa cgatggtcta tctccttgag cttggctatg tcaaagagat
     1081 agagcgtcga gtggatgaga aaatcgcgaa gcgtcttctc ctgggacccc gggaacacga
     1141 gggtcgcgtc ggggtagagc tttctcgccg ccaccatgga ggagagggag tcgaaatctg
     1201 cgttcatatg ggacgtgatg accttcatca acccctcggg tggtgcttct tgtggatctc
     1261 tttcaggcgc ttcgagtcga catgggtgta tatctgtgtt gtcgatatgt cctcgtgtcc
     1321 caggagcacc tggaccgacc tcaagtccgc gccgccctcg aggaggtgcg tggcaaaggt
     1381 atgccgtatc gtatgaggcg ataccttgcc tcttgacagc ttgagaccgt atttccgtat
     1441 gatcttccag acagcctggc gggttatcat gcctccgcgg cggttgggga agagatactg
     1501 gccctggggc ctttcctcat ccaggtaggc cttgacggca tccctcgagt aggagccaag
     1561 cggcacgacc cgttccttgg aacgcttt
//
LOCUS       DAOQTR010000529         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_106192_length_1586_cov_3.675376, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000529 DAOQTR010000000
VERSION     DAOQTR010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106192_length_1586_cov_3.675376"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKN85_10660"
     CDS             <1..989
                     /locus_tag="PKN85_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT44919.1"
                     /translation="KRTTAHEFRIVTRAGDIRWVMEAVTPITYSGRPAIFGHFMDITD
                     RKAMEEKLRESETLYRTIFETTGTATIIIEEDTTVALVNSEFERLYGAPKEHWEGKRS
                     WLEFAAEKERDRMLQYHNLRRRDPGAAPSRYEFRFVDRKGDVKDVLVTISMIPGTRRS
                     VSSHADITELKRQEKALIRREREIQAKSRNLEEMNTALKVLLKQREEDQRDMEEKVLA
                     NIKHLVIPYLGKLKTGRLGERERSCVKVLESNLLSIISPFSQRLSSKFLNLTPKELQV
                     ANLVKEGRTSKEIGELLDVSPATVALYRNRIRKKLSVTGKKVNLRTYLATLN"
BASE COUNT          343 a          504 c          455 g          284 t
ORIGIN      
        1 gaaaacgcac gacggcccac gagttccgca tcgtcacccg cgcgggggac atccgatggg
       61 tgatggaggc ggtcacgccg atcacctact cgggccggcc cgccatcttc ggccatttca
      121 tggacatcac ggaccgcaag gccatggagg agaagctccg tgagtcggag accctctacc
      181 gcaccatctt cgagacgacg ggcacggcca cgatcatcat cgaggaggac acgaccgtcg
      241 ccctcgtcaa ctcggagttc gagcgtctct acggggcgcc caaggaacac tgggagggca
      301 agcgcagctg gctggaattc gcggcggaaa aagaacgcga ccggatgctt cagtaccaca
      361 atctcaggcg ccgggacccc ggcgcggcgc cgagccggta cgaattccgc ttcgtcgacc
      421 ggaaaggcga cgtgaaggac gttctcgtca cgatctccat gatcccgggg acacgccgga
      481 gcgtctcctc ccatgccgac atcaccgagc tgaagcgcca ggagaaggcc ctgatccggc
      541 gcgaacggga gatccaggcc aagtcgcgca acctcgagga gatgaacacg gccctgaagg
      601 tcctgctcaa gcagcgcgag gaggaccagc gtgacatgga agagaaggtt ctcgccaaca
      661 tcaaacacct cgtgatcccc tacctgggca agctcaaaac gggccgcctc ggcgaaaggg
      721 agcgctcctg cgtcaaggtc ctggagtcga acctgctgag catcatctcg cccttctccc
      781 agcggctctc ctcgaagttt ctcaacctca ccccgaaaga gctccaggtg gccaacctcg
      841 tcaaggaggg ccggacgagc aaggagatcg gggaactgct ggacgtctcg ccggcaacgg
      901 ttgcactcta ccgcaaccgc atccgcaaaa agctgtccgt caccggcaag aaggtcaacc
      961 tccgcaccta ccttgcaacc ttgaactgag tgtatatttt tcctatacgg tttatcgtca
     1021 ttcttatccc attgcccttc ttgttttcct gttctacact gcaccccgtc atcggacgtc
     1081 aggctgtccg ccccgccagg aggttcttct caggcggttc tcgagacgcg tacgtgtgtc
     1141 cttcggcctg gcttcccggc gttgagtccc cacgccagga cgtcgaacaa cggaggtcat
     1201 cgctgccatg attctcacca gggccaatcg aatgctcccg atctgatttc atttggatct
     1261 catccgtatt gaggaggact gcgaacgcct atttgcggcg atcgaggaga gggcgtcgtc
     1321 tgccttccgg atgcagcccc tgcgcgtgca ggagcgcacg gccctgatga gccagtttta
     1381 cgggatccgg atatcccgct gcccggcgta cccgggcgac atctcggagc accctatgga
     1441 gtgcgcaggg acagagactc tgtgccttgt tacacataaa caatcctgta gaggagaccc
     1501 gttatgaatt ttgcgctgac cgaagagcaa cagatggtaa aggaccaggt aaccaagttc
     1561 gccgaaacga agatcaagcc catcgc
//
LOCUS       DAOQTR010000530         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_106223_length_1586_cov_2.849771, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000530 DAOQTR010000000
VERSION     DAOQTR010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106223_length_1586_cov_2.849771"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PKN85_10665"
     CDS             complement(<1..807)
                     /locus_tag="PKN85_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNT44920.1"
                     /translation="MQRKNDNRKAGDLQSLSLDELEAKLGSSADGLSRAEAQDRLQRY
                     GYNEIAEKKMNPFFKFLTYFWGPIPWMIEAAVILSAIVHRWPDFFIILVLLVANAAVG
                     FWEEFQAGNAIASLRARLAIQARVRREGEWTTVAARELVPGDVIRLRLGDIVPGDARL
                     FMGDPVEVDQSALTGESLPVTRKGGETVYSGSIIRQGEADGLVYGTGKNTYFGKTAQL
                     VEEAHTVSHFQRAVLKIGNYLIIIAIVLVAMILIVALFRGDRLGTTLQFAL"
     gene            1025..1249
                     /locus_tag="PKN85_10670"
     CDS             1025..1249
                     /locus_tag="PKN85_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44921.1"
                     /translation="MKAGALSVSLAERFTKAGHVGGQLKLLPEIDALPNPKKKRIVTP
                     AEEGASNHCIMENRSLMSRGLFDWPDEIFR"
     gene            complement(1265..>1586)
                     /locus_tag="PKN85_10675"
     CDS             complement(1265..>1586)
                     /locus_tag="PKN85_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HNT44922.1"
                     /translation="REIAGKMKAQEEARTGATIFTEIVPFIRFHLAEGYHQKYYLRGV
                     GDIAAEYLAVYPDLKDFIDSTAAARVNGYLGRYGTKAQLEEEIDSLGLSEKGRKRLLG
                     IIAR"
BASE COUNT          337 a          495 c          433 g          321 t
ORIGIN      
        1 cagggcgaac tgcagcgtcg tacccagcct gtcgccgcgg aagagggcga cgataaggat
       61 catggccacg aggacgatgg cgatgatgat caggtaattg cctatcttca ggaccgccct
      121 ctgaaaatgg ctgaccgtgt gtgcctcttc cacgagctgg gcagtcttgc cgaaataggt
      181 attcttcccg gtgccgtaga ccagtccgtc ggcctcgccc tgtcgtatga tcgatccgga
      241 atagacggtt tcccctccct tgcgtgtcac cggcagagac tcccccgtca gcgctgattg
      301 atccacctcc acggggtcac ccatgaacag gcgggcatcc cctggaacga tatcgccaag
      361 acggaggcgt atgacatcac cggggacaag ctcccgggcc gccaccgttg tccactcccc
      421 ctcgcgccgg acacgggcct gaattgcgag cctggccctg agtgacgcga ttgcgtttcc
      481 cgcctggaat tcctcccaga aacccaccgc cgcgttggca acgagaagaa ccagaatgat
      541 gaaaaaatcg ggccaccggt gcacgatagc cgacaggatc acggctgcct cgatcatcca
      601 tggtatcggt ccccagaaat aagtcaggaa cttgaaaaaa gggttcatct tcttctcggc
      661 gatctcgttg tacccgtaac gctggagcct gtcctgcgcc tccgcccggc tgagaccatc
      721 agcagacgat cccagtttcg cttccagttc gtcgagcgac agggactgga gatcccccgc
      781 cttcctgttg tcattcttcc tctgcatggc ctcttcctcc cgttgaaacc ctccatatcg
      841 cgcgccggtg cactgaataa gaaacagcaa tgacgtcggc aactttcgca aatatcccgt
      901 caatgacctt aggagagtat aaacgctttc tccaacaagg caacctcctc ctccgcgaag
      961 gcaggacaga aagcacaccg agactgggtg gcggggatca ttctcatgct cgacaaggca
     1021 cgacttgaag gcgggggcac tgtccgtctc cctcgcggaa aggttcacaa aagcaggtca
     1081 cgtgggcggg cagctgaaat tgcttccgga aatcgacgcc ctgcctaacc cgaagaaaaa
     1141 gcgcatcgtc acgcccgccg aagagggggc gtccaatcac tgcatcatgg agaaccgcag
     1201 tctcatgtcc cggggactat tcgactggcc cgacgagatc ttcaggtaaa agggcagcgt
     1261 atcctcatcg cgcgataatg ccgagaagtc ttttgcggcc tttctctgac aggcccagcg
     1321 agtcgatctc ttcttcgagt tgtgccttcg tgccgtagcg gccaagatat ccgttgaccc
     1381 gggcggccgc cgtggaatcg atgaaatcct ttagatccgg gtagacggca aggtattcag
     1441 cggctatatc gcccaccccc ctgagatagt acttctgatg gtagccctcg gcgaggtgga
     1501 accggatgaa cggcactatc tcggtgaata tggttgcccc ggtgcgggcc tcctcctgtg
     1561 ccttcatctt cccggcgatc tctctc
//
LOCUS       DAOQTR010000531         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_106499_length_1583_cov_4.713351, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000531 DAOQTR010000000
VERSION     DAOQTR010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106499_length_1583_cov_4.713351"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PKN85_10680"
     CDS             complement(<1..1268)
                     /locus_tag="PKN85_10680"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNT44923.1"
                     /translation="MRNTLFIMIFVLVVVGSAGADRGPVTWQENVSLSQESQKAIILH
                     NGTEEVLILGTELKASSEIDLLEFIPLPSDPEVGLAKGDPFAAVSRLISGKGLVFLAD
                     EFTTKGGPGGSSTVPVEIRFSEKVGIHDVTTVKINDIDGFSRWLEDFFRKKGIQTGRK
                     RLSGVLDTARDYMDRGYNYFVFDLVKVTERTRFIEPLMYRFRTGRIYYPLKTSNLIGG
                     KGAVEMVLVLPGSVTDDLWQGADKVFHMGAGAAIELSSSSKLNRADVQAIYPDRGFFG
                     PGSRIYLQVLRYRGQYRFKDDFIYDVGRLTPYAYRYESDTRFGAAKEFTPAFTGEERR
                     DLRDFFCPKSGDPQYIFRITDYRLDCWNFIPGEEYDVYAALFRGGLGGIPSGRVELEK
                     STTAKQLRGNRIKVDKYLAADFNGKYKVSCT"
     gene            1391..>1583
                     /locus_tag="PKN85_10685"
     CDS             1391..>1583
                     /locus_tag="PKN85_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44924.1"
                     /translation="MITVALPGGTGEITYRTGTLAVTPTEHIGKDPSTNHVPKNLRGV
                     ILAFVLSSLLVLASLFLSPP"
BASE COUNT          314 a          519 c          394 g          356 t
ORIGIN      
        1 gtgcagctta ccttgtactt gccgttgaaa tccgccgcaa gatacttgtc gaccttgatc
       61 ctgttgcccc ggagctgctt tgcggtggtg ctcttctcaa gctcgacccg gcccgatggg
      121 atgccgccca atcccccgcg gaacagcgcg gcatacacgt catattcctc gccgggaatg
      181 aagttccagc agtcaagccg gtagtcggtg atccggaaga tgtactgcgg gtcgccgctc
      241 ttcggacaga aaaaatccct gaggtcccgc ctctcttccc ccgtgaaggc aggggtgaac
      301 tccttcgccg cgccaaaccg ggtgtcgctc tcgtagcggt acgcgtaggg cgtcagtctc
      361 ccgacgtcgt agatgaagtc gtccttgaat ctgtactggc ccctgtacct cagcacctgc
      421 aggtagatcc tggaccccgg tccgaagaaa ccccggtcgg ggtagatggc ctgcacatcc
      481 gcccgattga gtttcgatga tgagctgagc tcgatcgctg ctccggcacc catatggaaa
      541 accttgtccg caccctgcca caggtcgtcc gtcacggacc cgggcaacac gaggaccatc
      601 tccacggcgc ccttccctcc tatgaggttg gacgtcttaa ggggataata gatcctgccc
      661 gtcctgaacc ggtacatcag cggctcgatg aagcgcgtcc tctcggttac cttgacgaga
      721 tcgaaaacga aatagttgta tcccctgtcc atgtagtccc gcgcggtgtc aagcacgccc
      781 gacagtctct tcctgccggt ttggataccc tttttcctga aaaagtcctc gagccatcgg
      841 gaaaacccgt cgatatcgtt gatcttcacc gtggtgacgt catggatccc aaccttctcc
      901 gaaaagcgga tctcgacggg gaccgtcgag gaaccccctg gcccgccctt cgtcgtgaac
      961 tcgtccgcta gaaagacgag accctttcca gagatcagcc gtgaaactgc cgcgaaggga
     1021 tcccccttcg caagtcctac ctcgggatcg gaaggcagag gtatgaactc caggagatcg
     1081 atctccgagc ttgctttcaa ctccgtcccg aggatcagca cctcttccgt gccgttatgg
     1141 aggatgatcg ccttttggga ttcctggctc aaactgacgt tctcctgcca cgtcacgggg
     1201 cccctgtcgg caccggctga acccacgacc acaagcacaa agatcattat gaacagggta
     1261 ttcctcatcg cctcactcct tcagcctcgt tacaggccgc attctccagc attctccaga
     1321 ttcaacacca tccacccgcg cgatgtccac actttcttta atacttttgt tcctccggtc
     1381 aggatgtgct atgattacag tcgcactccc cggcggcacc ggggagatca cctatcggac
     1441 aggtacattg gcggttacac caacggagca cataggcaaa gacccttcga cgaaccacgt
     1501 cccgaagaac cttcgcggcg tcatcctcgc tttcgtcctc tccagtcttc ttgtcctcgc
     1561 ttctcttttc ctttctcctc cat
//
LOCUS       DAOQTR010000532         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_106517_length_1583_cov_3.391361, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000532 DAOQTR010000000
VERSION     DAOQTR010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106517_length_1583_cov_3.391361"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1583)
                     /locus_tag="PKN85_10690"
     CDS             complement(<1..>1583)
                     /locus_tag="PKN85_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT44925.1"
                     /translation="VLESDQGNRFFISIKFPLRDQDGSVSGMCAMITEITARKSVEEQ
                     LAISRDRLSRAEIISRSGNWEFDMETKEVFMSPGARRIFGAGDAALTIPAMRGIPLPE
                     YRQVLNDALQGLIREGRPYDVEFKIRRPDTAEVVDIHSVAEYDGDRHVVFGIIQDITD
                     RKELESQLLQARKLEAIGTLAGGIAHDFNNILMGIEGYASLMMSEKTDAGHPDLERLK
                     SIEEQVKSASNLTRQLLGFARVGRYEIRPADMNEIVRETSAMFHRTRKELTIHEEYER
                     QPWTVEVDRGQIEQVLLNLYLNAWHAMPAGGEIRLTTANMVVDGIFASLHGIPTGKYV
                     RVSVADTGMGMGEETMKRIFDPFFTTKEMGRGTGLGLAMVYGIMKGHKGLVDVRSKPG
                     QGATFTLYFPASEKKLLRERRTVSKALRGSEAILLVDDESTVLRVTRRMLESLGYTVH
                     AMNGGSDAVSFLKDAKCAVDLVLLDMVMPGMSGSETFDRIRETNPSVKVILSSGYSMD
                     SEARMIMGKGCAGFIQKPY"
BASE COUNT          278 a          487 c          456 g          362 t
ORIGIN      
        1 ttgtaaggtt tttggatgaa gccggcacat cccttgccca tgatcatgcg ggcctcgctg
       61 tccatgctgt aaccgctcga gaggatgacc ttcacagagg ggttcgtctc cctgatgcgg
      121 tcgaaggtct cgctgccgga catgccgggc atgaccatat cgagcaatac gaggtcgacg
      181 gcgcatttgg catccttgag gaaggagacc gcgtccgatc cgccgttcat ggcgtgcacg
      241 gtgtatccga gagactcgag cattctccgc gtcacccgaa ggaccgtcga ttcgtcgtcg
      301 acaaggagga tcgcttcgga acccctcagg gccttcgata ccgttcgcct ttcccggagg
      361 agtttcttct ctgatgcggg aaaatagagg gtgaacgtgg ctccctggcc cggtttgctc
      421 ctgacatcca caagaccctt atgtcccttc atgatcccgt acaccatcgc gaggccgagg
      481 cctgtccccc tgcccatctc cttcgttgtg aagaagggat cgaatatgcg tttcatcgtc
      541 tcttctccca tgcccattcc cgtatccgcg acgctgaccc gaacatattt cccggtagga
      601 atgccgtgga gggaagcaaa gatcccgtcg acgaccatgt ttgcagtcgt gaggcggatc
      661 tctccccccg cgggcatggc atgccacgcg ttgaggtaga gattgagcag gacctgttct
      721 atctggcccc ggtcgacctc cacggtccac ggctgccttt cgtattcctc gtggatcgtc
      781 aactcctttc tcgttctgtg gaacatggcg gaggtctcgc ggactatctc gttcatgtcg
      841 gcaggccgta tctcatatct ccccacccgg gcaaagccca gaagctggcg ggtgaggttg
      901 gacgcgctct tgacctgttc ctcgatggac ttgagacgct cgagatcggg atggccggca
      961 tccgtctttt ccgacatcat cagcgaggcg tatccctcta tccccatgag aatgttgttg
     1021 aaatcgtggg caatgccgcc cgccagggtg ccgatggcct cgagtttcct ggcctggaga
     1081 agctgcgact cgagctcttt gcggtcggtg atgtcctgga tgatcccgaa caccacgtgc
     1141 ctgtcgccgt cgtactccgc cacggagtgt atatccacca cttccgccgt gtcggggcgc
     1201 cgtatcttga actccacgtc ataagggcgc ccttccctga tgaggccttg cagggcgtca
     1261 ttgagcacct gcctgtattc gggaaggggt attcccctca tcgcggggat cgtcagcgcc
     1321 gcgtcgccgg caccgaagat cctgcgggcc cccggggaca tgaagacttc cttcgtctcc
     1381 atgtcgaact cccagttgcc ggagcgggat atgatctccg cccgggacag gcggtctctg
     1441 ctgatggcaa gctgttcctc cacggatttg cgtgccgtga tctcggtgat catggcgcac
     1501 atgccgctca cggaaccatc ctgatcgcgc agggggaact tgatggagat aaagaaccgg
     1561 tttccctggt cgctttccag gac
//
LOCUS       DAOQTR010000533         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_108948_length_1562_cov_3.690776, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000533 DAOQTR010000000
VERSION     DAOQTR010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108948_length_1562_cov_3.690776"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..529)
                     /locus_tag="PKN85_10695"
     CDS             complement(155..529)
                     /locus_tag="PKN85_10695"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNT44926.1"
                     /translation="MNLQQVKDILDADVIVGSDQLEMEVKTAFGADLMSDVLAFAKPG
                     SLLLTGLTNPQVIRTSDVLDIAAIILVRGKRPLPETIQLAEELRIPLLSTKYILFESV
                     GRLYAKGIRGCIEKVGDARDLS"
     gene            complement(695..1180)
                     /locus_tag="PKN85_10700"
     CDS             complement(695..1180)
                     /locus_tag="PKN85_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44927.1"
                     /translation="MNHVMRSISLFLAAFILLSGVANAQWVFVGRKALGKVKQLTSEV
                     TDSASSQPADASRNIPRQGYDAATVLLEAPADKVYSTAVRVLQANKDIRISRKDDRAL
                     SIEFIDGSIAAGMQVTALSETVSQILVVSTSSGKQDTSIVLKGILRVCKEMGAHCQPA
                     D"
     gene            1319..>1562
                     /locus_tag="PKN85_10705"
     CDS             1319..>1562
                     /locus_tag="PKN85_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44928.1"
                     /translation="MSVRFPGERREYEKEDERPQLKPPLPTIEGVAPSCLRISSVEDR
                     NILEYLGERFPRIDAKTWIERMRRGRVVDESGNPLGP"
BASE COUNT          328 a          446 c          441 g          347 t
ORIGIN      
        1 gttcatctcc gcctcgtagg ttactatgga gactctccgg acggtctctt tcgggaggcc
       61 catgcctgtc agggtggcct tgacctcgcc ggagacgcgc ccggcaaagt cgaatcccgc
      121 ttccacggga aacgactttt caaatatcag ggattcacga gagatcacgt gcgtcgccga
      181 ccttttctat gcagccgcgg attcccttcg catagagccg gccaacggac tcaaaaagga
      241 tgtatttcgt tgataagagg ggtattcgca gttcctccgc aagctgtatc gtctccggta
      301 aggggcgctt tccgcgcacc agtatgatgg ctgcgatgtc gaggacatcg gacgtacgga
      361 taacctgagg atttgtaagg cccgtgagca gaaggcttcc cggcttggca aaggccagga
      421 cgtcgctcat caggtcggcc ccgaaggccg tcttcacttc catttccagc tgatcgctgc
      481 cgacgataac gtccgcgtca agtatgtctt tcacctgttg tagattcacc gtgctctcct
      541 ttcaacgacg ataggatatg ttaaaacagg gcgtctaatc aacctgaaat ttcacaaaag
      601 atacaacctg tgaaagatcg tttgttccgg agcgcgcgcc aacaatctcc ctctggggcc
      661 ggacccccgg cgcctgcgca ccgtggacct gccgtcaatc ggccggctgg cagtgcgcgc
      721 ccatctcttt acagacccgc agtattccct tgagcacgat ggatgtgtcc tgctttcccg
      781 acgatgtcga taccacgagg atctggctca ccgtctccga gagagcggtc acctgcatcc
      841 cggcggctat gctgccgtcg atgaattcga tgctgagggc cctgtcatcc ttcctgctga
      901 tcctgatgtc cttgttggcc tgcagcaccc tcacggcggt gctgtagacc ttgtcggcgg
      961 gggcttcgag aaggacggtg gcggcgtcgt atccctgccg ggggatgttc cttgacgcat
     1021 cggcaggctg cgacgaagca ctatcggtga cctcgctggt tagctgcttt accttcccca
     1081 gggccttcct tcccacaaag acccattgcg cgttggccac cccggaaagg agaatgaacg
     1141 cagcgagaaa cagggatatg gatcgcatga catggttcat aatgcttgcg cctccttcat
     1201 cttttgctca ttatccattc aaatgtgaac ttatacaagg ggttttttgg cgctttctgg
     1261 gcagttatga cgatctgtgg tacaatgggg aaacagaaag ccatgacgga agccacccgt
     1321 gagtgtgcgt ttccccggtg aaaggcgtga atatgagaag gaagacgaga gacctcaatt
     1381 gaagccccct ctgcccacca tcgaaggcgt ggcgccgagc tgcctcagaa tatcctcagt
     1441 ggaggacagg aacatactcg agtatctcgg ggaacgcttc cccagaatag acgcgaagac
     1501 ctggatcgag cggatgagaa ggggcagggt cgtcgacgaa tccggcaatc ccttgggccc
     1561 gg
//
LOCUS       DAOQTR010000534         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_109919_length_1554_cov_3.475650, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000534 DAOQTR010000000
VERSION     DAOQTR010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109919_length_1554_cov_3.475650"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKN85_10710"
     CDS             <1..320
                     /locus_tag="PKN85_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNT44929.1"
                     /translation="RPRDHYETDERLKEALDLIASGRFSRGDGSLFRPLVDTLLDRDE
                     YLLLADYASYIEMQDEVNRAYLDAERWVRMSILNVARTGKFSSDRSILEYAEGIWNVV
                     PGE"
     gene            complement(415..>1554)
                     /locus_tag="PKN85_10715"
     CDS             complement(415..>1554)
                     /locus_tag="PKN85_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNT44930.1"
                     /translation="QTVDDYDEAETDFEESIEFESPSGARDIIDFDIFEDIARARARL
                     EKMSCGFTVVRSKTVKKVRTGKKGKKWKQVVRYGKPELGSFTILLAVENVKTRDIRVI
                     RVHPRLGGRTESAIIEPGKANGVNTKFTIISPEHHVVLALKRPVRHGTTFREVVYTPY
                     SEGLDIPSVRKAGLEYLQGVIDNAKNDLIARRVRPASCNSFIDNDISVVLAIIEHIDP
                     QKFTSGRYTPEKLINETLVILGTNKHNAYRYSKSKAGAVGLFQFIPATYKRVQNLYPQ
                     AGLIRDFSQGMTSHENAAKASFLLFDADMNVLSDTRRSRLLDDPAATGRFLASAYNCG
                     PGRTRNSMERYGDNWASAVPTETKIYLRKFDAVLTWYRTGPFAAR"
BASE COUNT          272 a          443 c          469 g          370 t
ORIGIN      
        1 accgcccgcg cgatcactac gagacggacg aaaggctcaa ggaggctctt gaccttatcg
       61 cgtccggtcg tttctcacgg ggtgacggca gccttttccg gccactcgtc gatacgcttc
      121 tcgatcgtga cgagtacctt ctgctcgcgg actatgcctc ctacatagag atgcaggatg
      181 aggtgaacag ggcttacctt gatgcggagc gatgggtccg catgtccatc ctcaacgtgg
      241 caaggacggg gaaattctcg tcagataggt ccatcctcga gtatgcggag gggatctgga
      301 atgttgtccc gggggagtag ccgaggacgg cattcttacg gtgcaggcat atccgcggca
      361 tcccgttcac gttgcagcgg acatggggtt tcttatacgc cgggcggtgt cctgtcatct
      421 ggccgcgaag gggccggtgc ggtaccaggt gaggacggcg tcgaacttgc gcagatagat
      481 cttcgtctcg gtgggtacgg ccgaggccca gttgtctcca taccgttcca tgctgttcct
      541 ggtcctgccc ggcccgcagt tgtaggccga ggccagaaac ctgcctgttg cggccgggtc
      601 atcgaggagc cggctcctcc gggtgtcgct cagcacgttc atatcggcgt cgaaaagaag
      661 gaatgaggcc ttcgcggcat tctcgtggct tgtcatgccc tgggagaagt cccgtatgag
      721 gcccgcctgc gggtacaggt tctggaccct cttgtaggtt gccgggatga actggaagag
      781 acccaccgcc cccgccttcg actttgagta ccggtaggca ttgtgcttgt tcgtccccag
      841 tatgacgagc gtctcgttga tgagcttttc cggcgtgtac cgcccgctcg tgaacttctg
      901 gggatcgata tgctctatga tggcaagcac cacggaaatg tcgttgtcga tgaagctgtt
      961 gcaggaagcg ggcctcaccc gtctggcgat gaggtcattc tttgcattgt cgatcacgcc
     1021 ctgaaggtac tccaatcctg cctttcgaac ggaggggata tcaaggcctt ccgaataggg
     1081 ggtgtatacg acctccctga acgtcgtccc gtgacgaacg ggcctcttga gggcgagcac
     1141 gacgtgatgc tcgggggaga tgatggtgaa tttcgtgttg accccgttcg ccttgcccgg
     1201 ctcgatgatg gcgctttcag tgcggccgcc cagcctgggg tggaccctta tgacccgtat
     1261 gtcccttgtc ttcacgttct cgacggccag aaggatggtg aaggatccca gttcgggctt
     1321 tccgtaacgc accacctgtt tccatttctt tcctttcttg cccgtcctga ccttcttcac
     1381 ggtcttcgac cggacgacag tgaagccgca gctcatcttt tcaaggcgtg cccttgcccg
     1441 ggcgatatct tcgaagatgt cgaaatctat gatatcacgc gctcccgatg gggactcgaa
     1501 ctcgatggat tcctcgaaat cggtctccgc ttcgtcatag tcatccacgg tttg
//
LOCUS       DAOQTR010000535         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_110623_length_1548_cov_3.045546, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000535 DAOQTR010000000
VERSION     DAOQTR010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110623_length_1548_cov_3.045546"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..883)
                     /locus_tag="PKN85_10720"
     CDS             complement(410..883)
                     /locus_tag="PKN85_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNT44931.1"
                     /translation="MPVRKLKEFLDGNNVKYVTIAHSQAFTSQEIAASAHIPGKELAK
                     TVMIKVDGKMAMVVVPASHKVDFGLLKGVTGGQKVELATEDEFRYKFPECEIGAMPPF
                     GNLYGMDVFAAEELAEDETIAFNAGSHTELIKLAYSDFYRLVSPKIGRFTSKGNR"
     gene            complement(1007..>1548)
                     /locus_tag="PKN85_10725"
     CDS             complement(1007..>1548)
                     /locus_tag="PKN85_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44932.1"
                     /translation="FEFERSFYELRSRRFVPADELCKLTERAWAEWYGDSLSRPDSMF
                     WASKLHFSMVERSFYNYPYSFGYLFSLGLYARREAAGAGFLRAYKEILRDTGRMTAED
                     LIGKHLGEDITKPDIHEIASSRVADPKVADVWVGKGEAKFHASEFEEVADLGAVTVTK
                     AFYYSLGITITGGEVLHRF"
BASE COUNT          311 a          488 c          425 g          324 t
ORIGIN      
        1 cattcttctt atcatggacg gcctgtgata ttccatgggg agtaacatac aaggtccaat
       61 gttctgaacc ctggaaccgt ctgtattgta taccggccat ccggacccaa gtcaaggtat
      121 ggtcccgaac ccttgtaaga cacaccccgt tatggcatga gagagaaaaa ccaccgggag
      181 gtcccacggc tgcggccctc catattttcg ggcccggagg cccgtacaca cccatgaagg
      241 tatcactttc ggtcgtgacg ggcaaggagg cccgcgacat ctggcctcag aggacggggg
      301 atgaggcccg ggcgttcatt gaattcctga agtccgacag gggccatcaa atattcagga
      361 gtggggttgg gaatagaccc tccgccacat ccacgatggc cctgttggct tacctgtttc
      421 cctttgaggt gaaccgtccg atcttcggac tgaccagccg atagaaatcg ctgtaggcga
      481 gctttatgag ctccgtgtga gagcccgcgt tgaaggcgat ggtctcgtcc tccgcaagct
      541 cctcggccgc gaagacatcc atcccgtaga gatttccgaa agggggcatg gcgcctatct
      601 cgcactcggg gaacttgtac ctgaactcgt cctccgtcgc cagttcgacc ttctgccccc
      661 ccgtcacccc cttgagaaga ccgaaatcca ccttatgcga cgcgggtacg acgaccatcg
      721 ccatcttccc gtccaccttg atcataaccg tcttggccag ttcctttccc gggatgtgcg
      781 ctgaggcggc tatctcctgg gacgtaaagg cctgagaatg agcgatggtg acatatttta
      841 cattgttccc gtcgaggaac tccttcaact ttcgcaccgg catggcccac ctcctggggg
      901 atcgtatgtg tttcccatct atatagtata cacccaaccc gccgttttac gaatcgcccc
      961 gcgggaggtg cccgacccac cagggacagg aagccgcgcc ggcgtctcag aacctgtgca
     1021 ggacctctcc acccgttatg gttatgccca gactgtaata gaaggccttc gtgaccgtga
     1081 cggccccaag gtcggccacc tcttcgaact cggaggcatg gaacttcgcc tcccctttcc
     1141 cgacccacac gtcggccacc ttcgggtctg cgacacggct cgaggctatc tcatgtatgt
     1201 cgggcttcgt gatgtcctcg cccaaatgtt tcccgatcag atcctcggcg gtcatgcggc
     1261 cggtgtcgcg caggatctcc ttgtaggcgc gaaggaagcc cgcccccgcc gcctcccgcc
     1321 gcgcgtaaag gccgaggctg aacaggtaac cgaacgagta cgggtaattg tagaagctcc
     1381 gctccaccat gctgaagtgg agcttgctgg cccagaacat cgagtccggg cgcgacaggc
     1441 tgtcgccgta ccattctgcc caggcccttt cagtgagctt gcaaagctcg tcggcgggca
     1501 cgaagcgccg cgaacggagt tcgtagaacg atctctcgaa ctcgaagc
//
LOCUS       DAOQTR010000536         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_110957_length_1545_cov_3.083893, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000536 DAOQTR010000000
VERSION     DAOQTR010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110957_length_1545_cov_3.083893"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKN85_10730"
     CDS             <1..456
                     /locus_tag="PKN85_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT44933.1"
                     /translation="SLGGCGCFWQHMKGETPPPAAQPAKVAPPEQKVEVPVAVSPLKD
                     INFDFDKYAIRDADGAILKENMKWFQAKANAGKKVRIEGHCDERGTVEYNLVLGQKRA
                     DATKNFLVNLGADTRLLETVSYGKEKPVDPGHNEAAWAKNRRAHFLALQ"
     gene            581..>1545
                     /locus_tag="PKN85_10735"
     CDS             581..>1545
                     /locus_tag="PKN85_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNT44934.1"
                     /translation="MSLHTFRIPRYYLNLIDLSNPLCPIRGQAIPSLAEDGEGGSEDP
                     LGEEGISLTPIVLRRHPDRCVFLVSSECAMYCRFCNRRRFAGKSVDPEPYLEESYSAI
                     AEDASIREVILSGGDPFMLGAGKLASILERLRSMEKRLVIRISTRVPVVCPDILSPAH
                     LKAIEAASPVWVILHINHPREITERSLAAIGSIRKAGGIPVSQTVLLRNINDCPHVLL
                     RLFESLVMAGVKPYYLFQLDDVRGASHFKVRLERGRDIMAFLRLNASGLAVPQYALDI
                     PGGLGKVPVDSEHVRHQEGTTMVQLVSPSGEVGYYDNNAEESVCM"
BASE COUNT          341 a          426 c          453 g          325 t
ORIGIN      
        1 tcactcggtg gatgtggatg tttctggcag cacatgaaag gtgagacccc gcctcctgcc
       61 gcgcagccgg ccaaggttgc accgccggaa cagaaggttg aagttcccgt agcggtttcc
      121 ccgctgaagg acatcaactt tgatttcgac aagtatgcca tcagggatgc cgatggcgcg
      181 atattgaaag agaacatgaa atggttccag gccaaggcga atgccggcaa gaaggtcagg
      241 atcgaaggcc actgtgacga aagaggcacc gtggagtaca accttgtcct cggacagaag
      301 agagccgatg ccacgaagaa cttcctcgtg aaccttggcg cagatacaag gcttcttgaa
      361 acagtcagct atggcaaaga gaaaccggtt gatccgggcc acaacgaggc cgcctgggcg
      421 aagaatagaa gagcccattt tctggcgctg cagtaagaca aaaaggaaaa gtgaggagga
      481 gggtactata accgataccc tcccttttct tttctccctc agtgactcag acattgcttc
      541 cacattcgac cccgggaccg taagggccct tgagagggtc gtgtcgcttc ataccttcag
      601 gatcccccgg tactatctga accttatcga tctttcgaat cccctgtgtc ccatcagggg
      661 gcaggccatt ccgtcgctcg cggaggatgg tgaagggggc agcgaggacc ctcttggcga
      721 agaaggaata tcgctgaccc cgatagtgct gaggagacac cccgaccgat gcgtctttct
      781 cgtgagttct gaatgcgcga tgtattgccg cttctgcaat cgcaggcgat ttgcgggaaa
      841 atcggtggac cccgagccct atcttgaaga gtcgtatagc gccatagcgg aggatgcctc
      901 gatccgcgag gttatccttt cggggggcga ccccttcatg cttggcgcgg gaaagctggc
      961 ctcgatcctg gagcgtttga ggtccatgga gaaacgtctc gttatcagga tcagcacgcg
     1021 cgttcctgtt gtctgccccg atatcctgtc tccggcccac ttgaaggcca tcgaggctgc
     1081 ttccccggta tgggttatcc tccatatcaa tcatccccgg gagataacgg agcgatctct
     1141 cgcagctatc ggcagcatcc gcaaggcggg gggcatcccc gtaagtcaga cggtcctgct
     1201 caggaacatc aacgactgcc cccatgtgct cttgaggctt ttcgagtccc tggtcatggc
     1261 tggtgtgaaa ccctattacc ttttccaact cgacgacgtg cggggggcgt cgcacttcaa
     1321 ggtgaggttg gagcgcggcc gggatatcat ggcctttctc aggctgaacg cctcaggcct
     1381 tgccgtgccg cagtatgccc ttgacatccc cggcggcctc ggcaaggtcc cggtagactc
     1441 cgaacacgtg cggcatcagg agggaacaac gatggttcaa ctcgtaagcc cttcgggtga
     1501 ggtaggttat tatgacaaca acgctgaaga gagcgtgtgc atgag
//
LOCUS       DAOQTR010000537         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_111091_length_1544_cov_2.859637, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000537 DAOQTR010000000
VERSION     DAOQTR010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111091_length_1544_cov_2.859637"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKN85_10740"
     CDS             <1..389
                     /locus_tag="PKN85_10740"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNT44935.1"
                     /translation="FTRVILDNISSPLIFLVVTNLIILVAGMLMDPSPAIILFAPLLL
                     PAVHAFGISPIFFGALMVTNLAIGLVTPPVALTLYVAAEICQVSLSRLIKRVMPIVVL
                     LVIGLFLVTFIPDIAMFIPRLLMGVR"
     gene            497..1387
                     /locus_tag="PKN85_10745"
     CDS             497..1387
                     /locus_tag="PKN85_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNT44936.1"
                     /translation="MWNVESMGKIVDDMIYRSTTTISPDVRALMEKAFAEETNDTAKS
                     MLQAMLENERMAGEKVKPVCQSPGFPTVYVSFGDKSLPGCDLKKVWAEALVRGTNRQL
                     LRPSMVHPLTRENPGDNSGVGVPNFEIDYVPDQEYLDMLVSFKGCGAELANAMQIFTV
                     AKLEKGNDYAGLKRWVLDAVIKGGGKPCPPAAIGIGLGGQMDVACKLARKAVSVRRWD
                     DVNPDPLYGRLEKELYEAANSLGIGPAGTGGKTTLLAVKIEGASTHTAIAPAAITFHC
                     WTARRAGVRMYPDGRVETTL"
BASE COUNT          325 a          420 c          490 g          309 t
ORIGIN      
        1 cctttaccag ggtcatcctg gacaacatca gcagcccctt gatattcctt gtggtaacca
       61 acctgatcat cctggtcgcc ggaatgctga tggacccctc gccggcgatc atccttttcg
      121 cacccttgct cctgccggcg gtgcatgcct tcgggatcag ccccatcttt ttcggggcct
      181 tgatggtcac gaaccttgcc atcggccttg tcacgccgcc tgtcgcgctt accctttacg
      241 tcgcggcgga gatctgccag gtgagcctgt cgaggttgat caaaagagtt atgcccattg
      301 tcgtcttgct ggtcatagga cttttcctgg tgacctttat cccggatatt gcgatgttca
      361 tcccgagact tctgatgggg gtgcgataaa gatagccccg tgggcgaagg gggaaataat
      421 ttttcccagc ctgtttcggg atcaacccta ttctcaagga aaaaggggag agtattcccc
      481 acaaggaggg attcatttgt ggaacgttga atcaatgggc aagatcgtcg acgacatgat
      541 ctaccggtcg acgacgacga tatccccgga cgttcgggcg ttgatggaga aggcctttgc
      601 cgaggagacg aacgatacgg ccaagagcat gctccaggcg atgcttgaga acgagaggat
      661 ggccggtgag aaggtgaagc ccgtctgtca gtctcccggt ttcccgacgg tgtacgtcag
      721 ttttggcgac aagagcctgc ccggctgcga cctgaagaag gtctgggcgg aggcgctggt
      781 gaggggaacc aaccgtcagc ttctacgacc cagcatggtt caccccctga cgcgggagaa
      841 ccccggtgac aactcggggg tgggcgtgcc caactttgag atcgattatg tgccggacca
      901 ggaatacctt gacatgctgg tcagcttcaa aggctgcggt gcggaactgg ccaacgccat
      961 gcagatcttc acggtggcca agcttgagaa gggcaacgac tacgccgggt tgaagcggtg
     1021 ggtccttgac gcggtcatca agggtggcgg caagccctgc cctccggcgg cgataggcat
     1081 aggcctcggc ggccagatgg acgtggcctg caaactggcg aggaaggccg tgagcgtgag
     1141 gcgttgggac gacgtgaacc ccgacccgct ttacgggagg ctcgagaagg agctttacga
     1201 agcggctaac agcctcggca taggcccggc ggggacgggc ggcaagacga cgttgctggc
     1261 ggtgaagatc gaaggggcgt caacgcacac ggcgatagct cccgcggcga taaccttcca
     1321 ctgctggacg gcgcgcagag cgggcgtcag gatgtaccct gacggccgcg ttgagacgac
     1381 actttagggt ggtgaggaag atggccgaca agatcctgga tatacctcta agcgaagaag
     1441 agatcagaag ccttcatgtg ggcgatgtgg tctacctcag gggcccgata tacacatcga
     1501 gggacatggg tcatttcacc cttaagcagt acctcgacgc accg
//
LOCUS       DAOQTR010000538         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_111180_length_1543_cov_3.816532, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000538 DAOQTR010000000
VERSION     DAOQTR010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111180_length_1543_cov_3.816532"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PKN85_10750"
     CDS             complement(<1..928)
                     /locus_tag="PKN85_10750"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNT44937.1"
                     /translation="MRQKHGSIGTWFVLFILFTFLSAGMAYGQAKKYEVEISGLWTGT
                     NPYTIAVYWAELINKNSKMVKAIAREGRGPAVDMKTLIMKPEKRKSLVFFQVEDEVWA
                     AKRQIGDWKSFAGKYDLDNFRHIAFGGFTVDVLLTTNPKIRTLAEMNGKSTVVSNVSK
                     ASSKAVAFEEIFKTAGVKPKYQFLGMNAMTDAARDGTVDVIHGGINPVGSGKWEPSPY
                     LNELFAMKEVYAISIDRSVLTKMKQATGHPGTIVTIPAGNIGKNQKEPTTALGKCMNW
                     GVDASMPDEVVTEILRVYLENVDKFQQLSPAAR"
     gene            1329..>1543
                     /locus_tag="PKN85_10755"
     CDS             1329..>1543
                     /locus_tag="PKN85_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44938.1"
                     /translation="MEIEGKNVLVLGAYGEAGIAVCRQILRYLPAELVVTSLTEGEAQ
                     WALKELAPETPSYCKVMASSGNIFVRWS"
BASE COUNT          293 a          465 c          413 g          372 t
ORIGIN      
        1 cccgcgcggc cgggctcagc tgctgaaact tgtccacgtt ctccagatag accctcagga
       61 tctccgtcac gacctcgtcg ggcatggaag cgtcgacgcc ccagttcatg cactttccga
      121 gcgcggtggt cggttctttc tgattcttgc cgatattgcc cgcggggatc gttacgatgg
      181 tgcccggatg acccgtcgcc tgcttcatct tcgtcaggac cgatctgtcg atcgagatcg
      241 cgtagacctc cttcatggca aaaagctcgt taagatatgg agagggctcc cacttgccgg
      301 aaccgacggg gttgatcccg ccatggatca catcgactgt tccgtccctc gcggcatccg
      361 tcatggcgtt catgccgagg aactggtatt tcggtttcac cccggcggtc ttgaatatct
      421 cctcgaacgc gaccgccttg gatgaggcct tggagacgtt ggagacgacg gtgctctttc
      481 cgttcatctc ggccaaagtc ctgatcttcg gattggtggt caggagaacg tctaccgtga
      541 aaccaccgaa ggcgatgtgg cggaaattgt caagatcata tttcccggca aagctcttcc
      601 aatcgcctat ctgccgcttg gcggcccaca cctcgtcttc gacctggaag aagacaaggc
      661 tctttctctt ctccggcttc atgatgaggg tcttcatatc aaccgcaggg cctcttcctt
      721 cccgggcgat cgccttgacc atcttgctgt tcttgttgat gagctctgcc caatacaccg
      781 ctatggtata cgggttggtg cccgtccaga gccccgagat ctcaacctcg tacttctttg
      841 cctgcccgta tgccataccg gccgacagga aggtaaagag gatgaacagc acgaaccagg
      901 tgccaatcga accatgcttc tgtctcatct cgtctacccc tttcaattgt atttgttgtg
      961 ctcatccttg ccggagaaac gccctgaccc gccattcctc aacgggcttg ctcctctccg
     1021 gtaaagagtg aaaaccccgc gggtcccggc agccgcgatc gccgcaagac aggcggcagg
     1081 aggcacgcat ggccggtgga tattccatgg ccatctaccc ccacaccggc gtcctgattc
     1141 ccttttggtc taggcgatat gttggtccta ccataatagg taggttatat catcaagcac
     1201 cggtcgattg tcaagagatt ttttgcggcc tgccttattc ccggacaatc cctgcgcatc
     1261 ccggatttta tgcttgcccg ccacttgctt tcttgtgata taactacaga aatcatcagg
     1321 gggtgatcgt ggagatcgaa ggcaagaatg ttcttgtgct cggcgcctac ggcgaggctg
     1381 gcatcgcggt ctgccggcag atcctgcgat accttcccgc cgagctcgtg gtgacatccc
     1441 tgaccgaagg ggaggcgcag tgggcactga aggagctcgc ccccgagacc ccgtcctatt
     1501 gcaaggtcat ggcgtcgtca gggaacatat ttgtcaggtg gtc
//
LOCUS       DAOQTR010000539         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_112052_length_1536_cov_2.771776, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000539 DAOQTR010000000
VERSION     DAOQTR010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112052_length_1536_cov_2.771776"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKN85_10760"
     CDS             complement(<1..426)
                     /locus_tag="PKN85_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT44939.1"
                     /translation="MVDRGFVIRQMTLEDVEVAVEWAAEEGWNPGLSDAACFYHADPG
                     GFFIGEVDGRPVGCICAVSYGEAFGFIGFYIVRKDLRGKWYGVELGRRAMTRLGDRNV
                     GIDGVVAKIRNYEKFGFALAHRNIRYEGRGGAVSGLAGIV"
     gene            complement(464..1432)
                     /locus_tag="PKN85_10765"
     CDS             complement(464..1432)
                     /locus_tag="PKN85_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNT44940.1"
                     /translation="MAGGREIKAKGMNVKKFIEEKAAEIQAAVGKGVAINALSGGVDS
                     SVVTMLGHKALGERLKTYFIDTGLMRKDEPQRIQAIFKSLGVTIEIVDAKAKFFKALK
                     GLTDPEEKREAITQTFYKDVFGKLVTKSKAKYLLQGTILTDIDETVAGIKRQHNVFEQ
                     LGIDPKKAFGYHILEPLVQLRKDGVRKVGSALGLPASMYKRIPFPGPALAARVVGEVT
                     PEKVEIVRLATVVVEEELAKVKAFQYLAILHEDRMTGMRDGRRDFGRQIEVRCWDSVD
                     ARKATPTNLPFSKLTRIASRICKEVPGVVSVTYNIAAKPPSTIEAI"
BASE COUNT          264 a          532 c          390 g          350 t
ORIGIN      
        1 aacgataccc gctaaacccg ataccgctcc cccccggcct tcgtaccgta tgttcctgtg
       61 ggcgagcgcg aaaccgaact tctcgtagtt ccggatcttc gccaccaccc cgtctatgcc
      121 gacgttccgg tcgccaaggc gggtcatcgc ccgccttccg agctcaacgc cataccattt
      181 cccgcgcagg tctttcctga cgatgtagaa gccgatgaaa ccgaaggcct ctccatagga
      241 cacggcacag atgcatccta ccggtcggcc gtccacctcc ccgatgaaga aaccaccggg
      301 gtccgcgtga taaaagcagg cggcgtcgct gaggcccggg ttccacccct cctcggccgc
      361 ccattccacg gcaacctcaa catcctcgag cgtcatctgc ctgatgacaa atccccggtc
      421 gaccatccgt tccttctcct cgcccttgaa ccttgaactt tctttatatc gcctctatcg
      481 ttgaaggcgg tttcgcggcg atgttgtagg tcacgctcac gacaccggga acttccttgc
      541 agatcctcga ggctatgcgg gtaagcttcg agaaggggag gttcgtcggc gtggccttgc
      601 gggcgtccac ggaatcccag cacctgacct cgatctgccg gccgaagtcc cttctcccat
      661 cgcgcatccc cgtcatcctg tcctcgtgga ggatggcgag gtactggaag gccttcacct
      721 tcgcaagctc ttcctcgacg acgaccgtgg cgagcctcac gatctccacc ttttccggtg
      781 tgacctcacc gacgacgcgg gcggccagcg ccggacccgg gaaaggtatc ctcttgtaca
      841 tagacgcggg aagacccagg gccgaaccga ccttcctcac gccgtccttc cggagctgca
      901 cgagaggttc caggatatga tagccgaagg ccttcttagg gtctatgccg agctgttcga
      961 agacgttgtg ctgcctcttg atacccgcga ccgtctcgtc gatgtcggtg aggatcgttc
     1021 cctggagaag gtatttcgcc ttgctcttcg tgacgagctt gccgaagaca tccttgtaga
     1081 atgtctgggt tatggcctcg cgtttttcct ccgggtccgt caaccccttg agcgccttga
     1141 aaaacttcgc cttcgcgtcg acgatctcta tggtgacgcc gaggctcttg aatatcgcct
     1201 gtatgcgctg cggctcgtct tttcgcatga ggccggtgtc gatgaaatag gtcttcagcc
     1261 tctcacccag cgccttgtgg cccagcatgg tgaccaccga tgaatcgacc ccgcccgaca
     1321 gggcgttgat tgccaccccc ttgccgacgg ccgcctgtat ctcggccgcc ttttcctcga
     1381 tgaacttctt cacgttcatc cccttcgcct tgatctccct gccacccgcc atcagctccc
     1441 tcctttgata ttttcttttg tctgcagcct gagaccggga acctgaaact tgagaccgtc
     1501 tttcccgccc gtcacccatt acccgcccct ggccct
//
LOCUS       DAOQTR010000540         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_112706_length_1531_cov_2.213415, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000540 DAOQTR010000000
VERSION     DAOQTR010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112706_length_1531_cov_2.213415"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PKN85_10770"
     CDS             complement(<1..701)
                     /locus_tag="PKN85_10770"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNT44941.1"
                     /translation="MFDVPLNKLVPEYIKKFEAYIPSRPDPELMRLYKVDHLWRLNNN
                     ENPLGPPEGSARIIAEFPPARASIYPSGDSWYLRVKLAEKFDLDPDQIVFGNGANEAI
                     AFVIKAFCQEGDNIITADKTFAVYEWVAAFSGFEARLVPLKDFGFDDEGMLGRIDDRT
                     KILFVCNPNNPTGTYWDETRLRRFLDKVAGRQIVVVDEAYCEFVEADDYPDAMKLLKE
                     YPNLVTFRTFSKMYGL"
     gene            complement(712..>1531)
                     /locus_tag="PKN85_10775"
     CDS             complement(712..>1531)
                     /locus_tag="PKN85_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44942.1"
                     /translation="EAEDLKMFVRSIAGRYDPFVYPQDLRYPGKVVDMVASGEYGVLT
                     ATDRKGRMAGGMTWRSTGTSTIECYGPYVFAEEGRQNVGEGLVTALISTVARSEAICL
                     MNRYPTAELPPGYFEKLGTIEFALNGEKRPWDFLFRELKEDPGCRVYAHAGMEDFLKT
                     RYKRHFLAREIIPVKYEGERQNPHSVFGARFQHSQNLVVLSPLWDGADARENLARHIE
                     VLKAEGIANILFRIDLGHGWHARLYPVIEANGFVPSIVIPYQGKADWLFFTLRD"
BASE COUNT          298 a          467 c          440 g          326 t
ORIGIN      
        1 agaccgtaca tcttggaaaa ggtccggaag gtcacaagat tggggtactc tttgaggagc
       61 ttcatcgcgt cgggatagtc atcggcctcg acgaattcgc agtacgcctc gtcgacgacg
      121 acgatctgtc ttccggcgac cttgtcgagg aaccttctca gtctcgtctc gtcccagtag
      181 gtccctgtgg ggttgtttgg attgcacacg aagagtatct tcgtcctgtc gtctatgcgg
      241 ccaagcatcc cctcgtcgtc aaaaccgaaa tccttgagcg gcacgagcct tgcctcgaaa
      301 ccggagaacg cggccaccca ttcatagacg gcaaaggtct tgtcggccgt tatgatgttg
      361 tccccttcct gacagaaggc cttgatgaca aaagcgatgg cctcgttcgc cccgtttccg
      421 aacacgatct ggtcggggtc gaggtcgaac ttctcggcca gcttcacgcg caggtaccag
      481 gaatcgccgc tgggatagat gctcgccctc gccggcggaa actcggcgat gatacgcgcc
      541 gagccctcgg gagggcccag ggggttctcg ttgttgttga gccgccacag gtggtccacc
      601 ttgtaaagtc tcatgagctc ggggtcggga cgggacggga tgtacgcctc gaacttcttg
      661 atgtattcgg gaacaagctt gttgaggggg acgtcgaaca cgcgaaagac ctcaatcccg
      721 cagggtgaaa aagagccagt ccgccttgcc ttggtagggg atcacgatgg acggcacgaa
      781 cccgttcgcc tcgatgacgg gatagagccg cgcgtgccac ccgtggccga ggtcgatcct
      841 gaagaggata ttcgctatcc cttcggcttt caagacttcg atgtgcctcg ccaggttctc
      901 ccttgcatcg gcgccgtccc acaggggtga aaggaccacg aggttctggc tgtgctggaa
      961 cctggcaccg aagacggagt gggggttctg cctctcaccc tcatacttca cgggaatgat
     1021 ctccctggcg agaaaatgcc ttttgtaccg ggtcttgagg aagtcctcca tgccggcgtg
     1081 ggcgtagacc ctgcaccccg ggtcttcctt cagctcccgg aagagaaaat cccaaggcct
     1141 cttttccccg ttgagggcaa attctatggt acccagcttt tcgaagtaac cagggggcaa
     1201 ctccgccgtc ggatacctgt tcatgagaca gatcgcctcc gacctggcga cagtcgagat
     1261 aagcgccgtg acgagacctt cacccacgtt ctgccttccc tcttcggcga atacgtatgg
     1321 accgtaacat tcgatcgtcg aagtccccgt gctgcgccac gtcatgcctc cagccattct
     1381 ccccttcctg tccgtggcgg tcagcacccc gtactcgccg ctcgcaacca tgtcgacgac
     1441 cttgccggga taccggagat cctggggata gacgaagggg tcgtaccggc ccgcgatgga
     1501 ccggacaaac atcttcaggt cctccgcctc c
//
LOCUS       DAOQTR010000541         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_113763_length_1522_cov_3.413770, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000541 DAOQTR010000000
VERSION     DAOQTR010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113763_length_1522_cov_3.413770"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PKN85_10780"
     CDS             complement(<1..986)
                     /locus_tag="PKN85_10780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNT44943.1"
                     /translation="MKRCVAIVTAFLFLAASAGAFALTNEEERQYGKEIYLEIARSAP
                     INNDPYISLYLNEIRVRLESKTTMPFPVVLTVIDSPTIDAFATIGGYVYIAAGLIANC
                     DDEEEVAGVMAHEFAHIKKRHIAKRMGKQKYLNATMIATMLAAMLVGDSAREAVLVTG
                     MGTAQSMALKYSREDEDEADREGSILANEAGYGGLGIADFLKKLRAGGGDKLYPQYLL
                     THPYHESRIINLEKMWTRKPPSVDTSLFPYIIARAKVVQDYRSRGLADDIWVRRYARD
                     SKDPVAAYGAALYHSLKGNFEEAVRIARTINSPYRSVFLGEIYVTASRFPEAA"
     gene            complement(983..>1522)
                     /locus_tag="PKN85_10785"
     CDS             complement(983..>1522)
                     /locus_tag="PKN85_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44944.1"
                     /translation="LMTQRAREMGAIDTNFENASGLPSDNQYSTPYDLALIFRHAMAN
                     ERFVELIATRYFLFEDSTRKVRYKNHNRLLFCFEPAIGGKTGYTRAARHCYVGAFEKG
                     GRVYILALLGSRSLWGDTVEILKTIYDDVPTDKELRQARASSVTLCSTTAPKKKAASR
                     KKVVKHRTMKSKKKTVKKQ"
BASE COUNT          274 a          440 c          474 g          334 t
ORIGIN      
        1 gcggcctcgg gaaacctgct ggcagtgacg tagatctctc ccaggaagac gctcctgtaa
       61 ggagagttga tggtccgggc gatccgcacc gcctcttcaa aattgccctt cagggagtga
      121 tacagcgccg cgccgtatgc cgcgacggga tccttgctgt cccgggcgta ccgcctgacc
      181 cagatatcat ccgcaaggcc ccgggaacgg tagtcttgca cgaccttcgc ccgcgcgatg
      241 atgtagggaa agagagaggt gtccacggaa gggggtttcc tggtccacat cttctccagg
      301 ttgatgatgc ggctttcgtg ataggggtgg gtgagaagat actggggata gagcttgtcg
      361 cccccgccgg cccggagctt cttgagaaag tcggcaatgc cgagaccgcc gtagcccgcc
      421 tcgttggcaa ggatggaacc ctcgcggtcc gcctcgtcct cgtcctcgcg ggagtacttc
      481 agggccatcg actgcgccgt gcccatgccc gtcacaagga cggcctcacg tgccgaatca
      541 ccgacgagca tcgcggcgag catggtggcg atcatggtgg cgttgagata cttctgtttg
      601 cccatcctct tcgcgatatg ccttttcttg atatgggcaa actcatgggc catgacgccg
      661 gcgacctctt cctcgtcgtc gcaattggcg atgagacccg ccgcgatgta gacatatccg
      721 ccgatcgtgg caaaggcgtc aatggtgggg gaatcgatca cggtgaggac gaccgggaag
      781 ggcatggtcg tcttgctttc aagccttacc cgaatttcat tgaggtacag cgagatgtag
      841 ggatcgttgt tgatcggcgc cgaccgggcg atctccaggt agatctcctt gccgtactgg
      901 cgttcctcct cgttggtgag ggcgaaagca cccgcggacg ctgcaaggaa gagaaatgcc
      961 gttacgatgg ctacgcatct tttcattgtt tctttactgt cttcttcttc gacttcatgg
     1021 tacggtgctt gacgaccttt ttccgggatg ccgctttctt cttcggagcc gtcgtggaac
     1081 agagcgtgac ggaggacgcc cgcgcctggc ggagttcctt atcggtggga acgtcatcgt
     1141 atattgtctt cagtatctcc acggtgtcgc cccagaggct gcggctcccc agcagggcca
     1201 ggatatagac cctgccgccc ttctcgaagg cccccacgta acagtgtctc gccgcccggg
     1261 tataccccgt cttcccgccg atggcgggtt cgaagcaaaa gagcagcctg ttgtggttct
     1321 tgtagcgcac cttgcgcgtg gagtcctcga acagaaagta ccgcgtggcg atgagctcga
     1381 cgaagcgctc gttggccatg gcatgccgga atatgagggc caggtcgtag ggggtgctgt
     1441 actggttgtc cgagggaaga cccgaggcgt tctcgaaatt cgtgtcgatg gcacccatct
     1501 cccgggcgcg ctgcgtcatc ag
//
LOCUS       DAOQTR010000542         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_114376_length_1517_cov_2.547196, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000542 DAOQTR010000000
VERSION     DAOQTR010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114376_length_1517_cov_2.547196"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PKN85_10790"
     CDS             <1..1225
                     /locus_tag="PKN85_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT44945.1"
                     /translation="WLLLSAVCVGLAAGTKYNAFIAWIFLNGAVCFLYARDTGKQLQA
                     LRWGGLFFLVALAVVSPWLVKNLVLKGNPVYPLMDGLFTFIHGGREPAAFLEAGDVES
                     GGFSFIRNRMWVYGEEAWEILLLPIRIFFEGRDHIPQHFDGVLNPLFALAIPFAFAGS
                     WKAHRIFFLSLIAFVFTVSVLTADLRIRYILPILPFAAILAVMGIRNAVEWLQRRLNR
                     AQNAAAGVLMAGVALLMAANLLYLGKLFVDIRPLPYILGEESRDAFLTRQVGTYPAVA
                     FINQNLPEEAVVYLLYLSGRGYYLDREYLHHVGLETGLVKAIARSSADAATLPVFLRS
                     LGATHLLVQERLLVKAIVDNVPEEAVGNVLEKLARCLIKVYESNGYAVYEIKGTLLTD
                     VTDSTANVVSANESA"
     gene            1254..>1517
                     /locus_tag="PKN85_10795"
     CDS             1254..>1517
                     /locus_tag="PKN85_10795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT44946.1"
                     /translation="MFAGKKVVVVMPAYNAAQTLRKTYEEVMAQGVVDRVIVVDDASR
                     DETVALARTLHGVTVHRHHENRGYGANQKTCYREALKRGADIVV"
BASE COUNT          276 a          448 c          469 g          324 t
ORIGIN      
        1 atggctcctt ctctccgccg tctgcgtggg cctggcggcg ggcacgaaat acaacgcctt
       61 catcgcctgg atcttcctca atggggccgt ctgctttctg tacgcgcggg acacggggaa
      121 acagctgcag gccctgcgat ggggagggct gttttttctt gttgccctgg ctgtcgtttc
      181 cccctggctg gtgaaaaacc tggttttgaa gggaaacccg gtctatcccc tgatggatgg
      241 cctgttcaca ttcatccacg gaggccgtga acctgccgcc tttctggaag ccggcgatgt
      301 ggaaagcggc ggcttcagct tcatacggaa caggatgtgg gtctatgggg aagaagcttg
      361 ggagatcctg ctgctgccga tccggatctt cttcgagggc cgggatcaca tcccgcagca
      421 tttcgacggc gtcctgaacc cgctgtttgc cctggcaatt ccgtttgcct ttgcggggag
      481 ttggaaggca cacaggatct tcttcctgtc tctcattgcg ttcgtcttca ccgtgtccgt
      541 cctgactgcg gatctgcgga tccgctacat ccttccgatc ctgcccttcg ctgcgatcct
      601 tgccgtgatg ggaatccgca acgccgtgga atggctgcag cgccgcttga atcgcgcaca
      661 aaacgcggca gcgggggtcc ttatggccgg tgttgccctg ctcatggctg caaacctcct
      721 ctacctgggg aagctgttcg tcgacatccg cccgctgccg tatatcctcg gggaagaatc
      781 gagagacgcg ttcctcacca ggcaggtcgg cacctacccg gccgtggcct tcatcaatca
      841 gaaccttccg gaggaggccg tcgtctatct cctctacctg agcggccgcg ggtattacct
      901 ggaccgggag tacctccatc acgtcggtct ggagacaggg ctcgtaaagg ccatcgcaag
      961 atcgtcggcc gacgccgcaa cgctcccggt tttcctgagg tccctggggg cgactcatct
     1021 gctggtgcag gagaggctgc ttgtcaaagc gatcgtggac aacgtcccgg aagaagccgt
     1081 cggaaatgtc ctggaaaagc ttgcgcgatg cctgattaag gtttatgaat cgaacggcta
     1141 tgctgtctat gaaatcaagg ggacgctgtt gactgatgtg actgactcta cggcaaatgt
     1201 tgtctcagcg aatgagtcag cgtagtcaac aacgtccctg atccgggggg agcatgttcg
     1261 cagggaagaa agtcgttgtc gtcatgccag cgtacaacgc ggcgcagacg ctccggaaga
     1321 cctacgagga ggtcatggcg cagggggtgg tcgaccgggt catcgtcgtc gacgacgcga
     1381 gccgcgacga gaccgtcgcc cttgcgagga cccttcacgg tgtcaccgtc caccggcatc
     1441 acgagaaccg gggctacggc gcgaaccaga agacctgcta ccgcgaggcg ctgaagcgcg
     1501 gcgccgacat cgtggtg
//
LOCUS       DAOQTR010000543         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_114965_length_1512_cov_3.622512, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000543 DAOQTR010000000
VERSION     DAOQTR010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114965_length_1512_cov_3.622512"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKN85_10800"
     CDS             <1..336
                     /locus_tag="PKN85_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNT44947.1"
                     /translation="LYMTAITLTTVGCGDVIGLDNKLLGKLFTIIYVFIGAGTIAYLF
                     TTLAAYIVEGELRKVFRRRPSRPRGKGTPGGARVRRFGQRAARSTPCCSLKIDNALQR
                     MIVFHERKR"
     gene            333..1109
                     /locus_tag="PKN85_10805"
     CDS             333..1109
                     /locus_tag="PKN85_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HNT44948.1"
                     /translation="MKIAVSGKGGAGKTTVAGVMARILGDRGRRVIAIDADPDSNLAS
                     AIGMDEEGLKGVKPLAAMDEFIAERTGAKKGQYGAFFQLNPRVDDIPERFSLVKDGVK
                     LLVLGSIPQGGGGCFCAENALLRSLLSYVIVERDEYVIVDLEAGLEHLGRGTTEYIDA
                     LIVVVEPGKRSFQTAHQVRRLADDIGIKKVYVVGNKIAGGQDESLVRENLEGLPLLGF
                     LSLNDRIIEADKRGVSPYDLSVKVREEVAAVLDSLEDLTG"
     gene            1106..>1512
                     /locus_tag="PKN85_10810"
     CDS             1106..>1512
                     /locus_tag="PKN85_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT44949.1"
                     /translation="MRRVTAVVWLVLSTITLSLVALLVALFGDREETIHRMARLWAKM
                     YLAVTGIEARIEGLERLGSPPYVFMVNHQSALDIYALLAGLPLSFKWIAKRQLFSIPV
                     FGWAIRKAGYISIDRENAREALKAIEKAAQRIR"
BASE COUNT          314 a          429 c          485 g          284 t
ORIGIN      
        1 ctgtacatga cggccattac gctcacgacc gtggggtgcg gagatgtgat cggtctcgac
       61 aacaagctct tgggaaaact cttcaccatc atctatgtct tcatcggggc cggcaccatc
      121 gcctatcttt tcacgaccct tgcggcgtac atcgtcgaag gcgagttgag gaaggttttc
      181 aggaggaggc catcgcgtcc ccgaggaaaa ggaactcctg gtggagctcg tgtccggcgg
      241 ttcggacagc gcgctgcgag gtccaccccc tgttgttcgc tcaagattga caacgctttg
      301 caaaggatga tagtttttca cgaaaggaaa cgatgaagat agctgtttca ggaaaaggcg
      361 gtgcggggaa gacgaccgtt gccggggtca tggcgcggat cctcggcgac cggggcagga
      421 gggtgatcgc catcgacgcg gacccggatt cgaacctggc aagcgccatc ggcatggatg
      481 aggagggtct gaagggcgta aaaccccttg ccgccatgga cgagttcatc gccgagcgga
      541 cgggcgcgaa aaagggccag tatggcgcct tcttccagct gaacccccgc gtcgacgaca
      601 taccggagcg cttctccctc gtgaaggatg gggtgaagct cctcgtgctg gggagcatac
      661 cccagggggg cggaggctgt ttctgcgccg agaacgccct cttgaggagc ctcctttcct
      721 acgtcatcgt ggagcgcgac gaatacgtca tagtggacct cgaagcgggg ctcgagcacc
      781 tgggaagggg tacgacggag tacatcgacg cgctcatcgt cgtcgtcgaa ccgggcaagc
      841 ggagttttca gacggcccat caggtcagaa ggcttgccga cgacatcggg ataaagaagg
      901 tctatgtcgt gggaaacaag atcgccggcg ggcaagacga gtccctcgtc cgtgagaacc
      961 tggagggact gcctcttctg ggattccttt ccctcaatga caggatcatc gaggccgaca
     1021 agaggggcgt gtcgccctac gacctcagtg taaaggtccg ggaggaagtg gcggcggtcc
     1081 tcgattccct tgaggacctc accggatgag aagggtcacc gcagtcgtct ggctggtcct
     1141 ctcgacgata accctctccc ttgtcgccct tcttgtggcc cttttcggcg atcgagagga
     1201 gaccatccac cgcatggcac gcctctgggc aaagatgtat ctcgcggtga ccggaataga
     1261 agcccgcatc gaagggctcg agcggctcgg ctcgcccccg tatgtcttca tggtgaacca
     1321 ccagagcgcc ctcgacatat acgcgcttct ggccgggttg ccgctgtcct tcaagtggat
     1381 cgccaagaga cagcttttca gcatccccgt cttcggatgg gcgatacgga aggcgggcta
     1441 catcagcata gaccgggaga acgcccgcga ggccctgaag gccatagaga aggccgcgca
     1501 gaggatacgg ga
//
LOCUS       DAOQTR010000544         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1135
            NODE_115224_length_1510_cov_3.089347, whole genome shotgun
            sequence.
ACCESSION   DAOQTR010000544 DAOQTR010000000
VERSION     DAOQTR010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852478
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:07:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,163
            CDSs (total)                      :: 2,148
            Genes (coding)                    :: 2,139
            CDSs (with protein)               :: 2,139
            Genes (RNA)                       :: 15
            tRNAs                             :: 13
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115224_length_1510_cov_3.089347"
                     /isolate="AMR_MDS_1135"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PKN85_10815"
     CDS             <1..1318
                     /locus_tag="PKN85_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNT44950.1"
                     /translation="IFVKGAPERVLDMCSSLPSGEAIDRAKVSRTAEHFAEDGLRVLA
                     MAYKDAGIDVNGDGFTRGRLGSDLILAGLQGMIDPPRPEAVDAIRGCREAGIRVAMIT
                     GDHAVTASAIGRMIGLGENRSPAITGRELERMSDDDLFGKVQETSVYARVSPQHKLRI
                     VKQYMRHGEIVAVTGDGVNDAPALKAAHIGAAMGRTGTDVAREAADMVVTDDNFASIF
                     HAVEEGRIVFDNIRKVTVFLIPTGFAAILSILACMFLGIPIPYVAAQLLWINLVANGL
                     QDVALAFEPGEKDIVKRQPRSPKEGIMSRLMLERSILVGVLISAGVIYNFHDALTQGS
                     SVEHARTIAMTTMVIFQFFHAWNSRSETRSVFRLNPLSNPFLFYSMIVASLAQIAVIY
                     VPTLQWVFRTDNLSGGEWLRIIAVAVSAIAAVEIEKLLRGKRRPKG"
BASE COUNT          308 a          448 c          466 g          288 t
ORIGIN      
        1 cattttcgtg aagggtgccc ccgaaagagt cctcgacatg tgttcgagcc ttccttcggg
       61 agaagccatc gacagggcaa aggtatccag aacagcggag cacttcgccg aagatgggtt
      121 gagggtcctc gccatggcct acaaagatgc cggaatcgac gttaacggag acgggttcac
      181 ccgcggccgt ctgggaagcg atctcatcct ggcgggcctc cagggcatga tcgatccacc
      241 ccggccggag gcggtggacg ccatcagggg atgcagggaa gcaggcatca gggtggcgat
      301 gatcacgggt gatcatgccg tgaccgcctc ggccatcggc aggatgatcg gcctggggga
      361 aaaccggtcg cccgccatca cggggagaga actggaaagg atgagtgacg acgatctctt
      421 tggcaaagtc caggagacca gcgtctatgc ccgggtctcg ccgcagcaca agctgcgcat
      481 tgtcaagcag tacatgcggc acggagagat cgtggccgtc accggagacg gcgtcaatga
      541 cgcccccgcc ctcaaggcgg cgcacatagg ggcggccatg ggaaggacgg ggaccgacgt
      601 cgcccgcgag gccgccgaca tggttgtgac cgacgacaat tttgcgtcca tcttccacgc
      661 tgttgaggag ggaaggatag ttttcgacaa tatacggaag gtgacagtgt tccttatacc
      721 cacggggttc gcggccatcc tttcgatcct cgcctgcatg tttctcggga tacccatccc
      781 ctacgtggcg gcccagctcc tttggatcaa cctcgtcgcc aatggcctgc aggacgtggc
      841 cctcgccttc gagccggggg aaaaggacat cgtcaagagg cagccgcgga gcccgaaaga
      901 aggtatcatg tcgcgtctga tgctcgagcg gagcatcctc gtcggcgtcc tgatctcggc
      961 gggtgtcatt tacaacttcc atgacgccct gacccagggt tcctccgtgg agcacgcccg
     1021 gacgatcgcc atgaccacta tggtcatctt ccagttcttc cacgcctgga actcacggtc
     1081 tgaaacccgg tccgtcttcc gtcttaaccc cttaagcaac cccttcctct tctacagcat
     1141 gatcgtggca tctctggctc agatcgccgt catctatgtg cccacgcttc agtgggtttt
     1201 caggacggat aatctctcgg gaggggaatg gctgcggata atcgccgtgg cggtcagcgc
     1261 gatcgcggcg gtcgagatcg aaaagctcct gcgcgggaag cgcagaccga aaggatgaaa
     1321 ggtggatcgc gctcttcacc cggcggacgg agagcacttg acctcggtcc cttcatactg
     1381 caataatacg gacgggaaaa gaacgtgacg gaacaggcct tctatgcgat agcgctgatc
     1441 ctcagcctta ccgcgatcct cggcgcggtg ggaatgttgc tgaggcagcc ccttatcgta
     1501 gccttcctgg
//
